Miracle-dz/UniFAR-FTUs huggingface dataset:
The Allantoic Core Domain: New insights into development of the murine allantois and its relation to the primitive streak

The whereabouts and properties of the posterior end of the primitive streak have not been identified in any species. In the mouse, the streak's posterior terminus is assumed to be confined to the embryonic compartment, and to give rise to the allantois, which links the embryo to its mother during pregnancy. In this study, we have refined our understanding of the biology of the murine posterior primitive streak and its relation to the allantois. Through a combination of immunostaining and morphology, we demonstrate that the primitive streak spans the posterior extraembryonic and embryonic regions at the onset of the neural plate stage (∼7.0 days postcoitum, dpc). Several hours later, the allantoic bud emerges from the extraembryonic component of the primitive streak (XPS). Then, possibly in collaboration with overlying allantois‐associated extraembryonic visceral endoderm, the XPS establishes a germinal center within the allantois, named here the Allantoic Core Domain (ACD). Microsurgical removal of the ACD beyond headfold (HF) stages resulted in the formation of allantoic regenerates that lacked the ACD and failed to elongate; nevertheless, vasculogenesis and vascular patterning proceeded. In situ and transplantation fate mapping demonstrated that, from HF stages onward, the ACD's progenitor pool contributed to the allantois exclusive of the proximal flanks. By contrast, the posterior intraembryonic primitive streak (IPS) provided the flanks. Grafting the ACD into TC/TC hosts, whose allantoises are significantly foreshortened, restored allantoic elongation. These results revealed that the ACD is essential for allantoic elongation, but the cues required for vascularization lie outside of it. On the basis of these and previous findings, we conclude that the posterior primitive streak of the mouse conceptus is far more complex than was previously believed. Our results provide new directives for addressing the origin and development of the umbilical cord, and establish a novel paradigm for investigating the fetal/placental relationship. Developmental Dynamics 238:532–553, 2009. © 2009 Wiley‐Liss, Inc.
Femtosecond time-resolved X-ray absorption spectroscopy of anatase TiO2 nanoparticles using XFEL

The charge-carrier dynamics of anatase TiO2 nanoparticles in an aqueous solution were studied by femtosecond time-resolved X-ray absorption spectroscopy using an X-ray free electron laser in combination with a synchronized ultraviolet femtosecond laser (268 nm). Using an arrival time monitor for the X-ray pulses, we obtained a temporal resolution of 170 fs. The transient X-ray absorption spectra revealed an ultrafast Ti K-edge shift and a subsequent growth of a pre-edge structure. The edge shift occurred in ca. 100 fs and is ascribed to reduction of Ti by localization of generated conduction band electrons into shallow traps of self-trapped polarons or deep traps at penta-coordinate Ti sites. Growth of the pre-edge feature and reduction of the above-edge peak intensity occur with similar time constants of 300–400 fs, which we assign to the structural distortion dynamics near the surface.
Product mix decisions and production lot sizes

According to the earlier research related to the product mix decisions, the approach called throughput accounting seems to provide much better results than conventional management accounting. However, all the examples so far have assumed that cycle times in used resources are not dependent on any other variable. In practice, the situation is not so simplified; the decisions related to production lot sizes will affect the cycle time requirement of a product in a particular resource, and, in the case of capacity constraint, the product's and system's profitability. The main cause for this is the setup time in a particular resource. As can be concluded from the results of this paper, the appropriate lot sizing decisions will enable system to produce more with its existing resources and therefore increase its profits significantly. As the performed sensitivity analysis suggests, the differences between traditional management accounting and throughput accounting will become increasingly insignificant after lot sizes have been somewhat increased. According to shown results it is being suggested that lot sizing decisions are rather important for the profitability of manufacturing unit, and especially in capacity constraint resource(s) the use of smaller lot sizes should be avoided.
Long‐Term Dosing of Arzoxifene Lowers Cholesterol, Reduces Bone Turnover, and Preserves Bone Quality in Ovariectomized Rats

Long‐term effects of a new selective estrogen receptor modulator (SERM) arzoxifene were examined in ovariectomized (OVX) rats. Arzoxifene was administered postoperatively (po) at 0.1 mg/kg per day or 0.5 mg/kg per day to 4‐month‐old rats, starting 1 week after OVX for 12 months. At study termination, body weights for arzoxifene groups were 16–17% lower than OVX control, which was caused by mainly reduced gain of fat mass. Longitudinal analysis of the proximal tibial metaphysis (PTM) by computed tomography (CT) at 0, 2, 4, 6, 9, and 12 months showed that OVX induced a 22% reduction in bone mineral density (BMD) at 2 months, which narrowed to a 12% difference between sham‐operated (sham) and OVX rats by 12 months. Both doses of arzoxifene prevented the OVX‐induced decline in BMD. Histomorphometry of the PTM showed that arzoxifene prevented bone loss by reducing osteoclast number in OVX rats. Arzoxifene maintained bone formation indices at sham levels and preserved trabecular number above OVX controls. Micro‐CT analysis of lumbar vertebrae showed similar preservation of BMD compared with OVX, which were not different from sham. Compression testing of the vertebra and three‐point bending testing of femoral shaft showed that strength and toughness were higher for arzoxifene‐treated animals compared with OVX animals. Arzoxifene reduced serum cholesterol by 44–59% compared with OVX. Uteri wet weight from arzoxifene animals was 38–40% of sham compared with OVX rats, which were 29% of sham. Histology of the uterine endometrium showed that cell heights from both doses of arzoxifene were not significantly different from OVX controls. In summary, treatment of OVX rats with arzoxifene for nearly one‐half of a lifetime maintained beneficial effects on cholesterol and the skeleton. These data suggest that arzoxifene may be a useful therapeutic agent for osteoporosis in postmenopausal women.
Efecto del riego y la fertilización nitrogenada sobre la calidad de planta de Pinus halepensis Mill. y su comportamiento en campo

El trabajo aborda la influencia del riego y la fertilizacion nitrogenada sobre la calidad de planta de pino carrasco obtenido en vivero y su comportamiento en campo mediante dos grupos de experimentos den condiciones controladas y uno en campo. En el primer grupo de experimentos se analizo por separado el efecto de las practicas de cultivo mencionadas sobre el endurecimiento de las plantas frente a la helada mediante la simulacion en filotron del descenso otonal del fotoperiodo bajo dos regimenes de descenso otonal de temperatura. Se midio la fluorescencia de la clorofila antes y despues de una helada ariticial a -5oC en diferentes tiempos a lo largo del experimento para evaluar dicho endurecimiento. La temperatura fue el principal factor que influyo sobre el endurecimiento. La fertilizacion nitrogenada produjo una mayor susceptibilidad a la helada en las mediciones intermedias, pero al final del experimento no se encontraron diferencias entre tratamientos de fertilizacion. La reduccion del aporte de agua solo produjo una ligera mayor susceptibilidad a la helada al principio del experimento. Se encontraron buenas relaciones entre el descenso de Fv/Fm unas horas despues de la helada y el dano visible en planta un mes despues. En el segundo grupo de experimentos se estudio el efecto del preacondicionamiento por reduccion de riegos y de la fertilizacion nitrogenada sobre la tolerancia a la sequia. Se realizaron mediciones continuas del diametro del tallo con sensores de desplazamiento lineal variable. Se encontraron diferencias en el reparto de la biomasa entro los tratamientos de preacondicionamiento al estres hidrico. No se encontraron diferencias entre tratamientos en la respuesta al ciclo de sequia con los sensores. En el experimento en campo, durante dos campanas de cultivo se midieron el tamano de la planta y la concentracion de nitrogeno antes de la plantacion en diferentes lotes de planta que diferian principalmente en la cantidad de nutrientes aportados durante el cultivo. Se realizo un seguimiento de la mortalidad y crecimiento en altura durante tres anos en la primera de las plantaciones y durante el primer ano en la segunda. La concentracion de nitrogeno fue el factor mas determinante en el crecimiento del primer ano en campo, especialmente en las condiciones mas desfavorables. En anos sucesivos, el factor determinante fue la altura alcanzada al final del primer ano. En ambas plantaciones se encontro una buena relacion entre el contenido total de nitrogeno en planta antes de la plantacion y el crecimiento posterior en altura. Un contenido de nitrogeno por debajo de 15 mg por planta resulto en un comportamiento deficiente en campo.
Outbreaks of meningococcal B infection and the 4CMenB vaccine: historical and future perspectives

Strains of Neisseria meningitidis serogroup B (MenB) causing invasive meningococcal disease are genetically diverse; however, only a small number of hyperinvasive lineages (CC32, CC41/44, CC269 and CC162) have dominated during the global spread over the past 50 years. Since the mid-1970s, major outbreaks and hyperendemic disease have been reported in Norway, Cuba, France, Canada, New Zealand (and elsewhere), most recently in the USA. We characterized the epidemiology of these MenB outbreaks and their associated clonal complexes and retrospectively assessed the potential coverage offered by the 4CMenB vaccine, a four-component vaccine developed to help confer protection against a broad range of meningococcal B strains causing disease. Of 21 isolates from four clonal complexes evaluated using both human Serum Bactericidal Assay and the Meningococcal Antigen Testing System, coverage ranged from 67 to 100%. 4CMenB shows good potential as a candidate vaccine to be used in the control of new MenB outbreaks globally.
Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery

Neurite initiation is the first step in neuronal development and occurs spontaneously in soft tissue environments. Although the mechanisms regulating the morphology of migratory cells on rigid substrates in cell culture are widely known, how soft environments modulate neurite initiation remains elusive. Using hydrogel cultures, pharmacologic inhibition, and genetic approaches, we reveal that paxillin-linked endocytosis and adhesion are components of a bistable switch controlling neurite initiation in a substrate modulus-dependent manner. On soft substrates, most paxillin binds to endocytic factors and facilitates vesicle invagination, elevating neuritogenic Rac1 activity and expression of genes encoding the endocytic machinery. By contrast, on rigid substrates, cells develop extensive adhesions, increase RhoA activity and sequester paxillin from the endocytic machinery, thereby delaying neurite initiation. Our results highlight paxillin as a core molecule in substrate modulus-controlled morphogenesis and define a mechanism whereby neuronal cells respond to environments exhibiting varying mechanical properties.
Injury in the National Basketball Association

Background: Injury patterns in elite athletes over long periods continue to evolve. The goal of this study was to review of the injuries and medical conditions afflicting athletes competing in the National Basketball Association (NBA) over a 17-year period. Design: Descriptive epidemiological study. Methods: Injuries and player demographic information were reported by each team’s athletic trainer. Criteria for reportable injuries were those that resulted in (1) physician referral, (2) a practice or game being missed, or (3) emergency care. The demographics, frequency of injury, time lost, and game exposures were tabulated, and game-related injury rates and 95% confidence intervals were calculated. Results: A total of 1094 players appeared in the database 3843 times (3.3 ± 2.6 seasons). Lateral ankle sprains were the most frequent orthopaedic injury (n, 1658; 13.2%), followed by patellofemoral inflammation (n, 1493; 11.9%), lumbar strains (n, 999; 7.9%), and hamstring strains (n, 413; 3.3%). The most games missed were related to patellofemoral inflammation (n, 10 370; 17.5%), lateral ankle sprains (n, 5223; 8.8%), knee sprains (n, 4369; 7.4%), and lumbar strains (n, 3933; 6.6%). No correlations were found between injury rate and player demographics, including age, height, weight, and NBA experience. Conclusion: Professional athletes in the NBA experience a high rate of game-related injuries. Patellofemoral inflammation is the most significant problem in terms of days lost in competition, whereas ankle sprains are the most common injury. True ligamentous injuries of the knee were surprisingly rare. Importantly, player demographics were not correlated with injury rates. Further investigation is necessary regarding the consequences and sport-specific treatment of various injuries in NBA players. Clinical Relevance: Knowledge of these injury patterns can help to guide treatments and provide more accurate guidelines for an athlete to return to play.
Maternal Stress and Mothering Behaviors in Stable and Unstable Families

Prior research linking family structure with parenting often confounds the effects of family structure with the effects of family instability. In this analysis, I use data from the Fragile Families Study (N= 3107) to answer two main questions: (a) Are there differences in maternal stress and mothering behaviors across stably married, cohabiting, visiting, and single-mother families? and (b) Does family instability have a negative impact on mothering behaviors? I focus specifically on the relationship between the biological parents of a one-year-old focal child. Most differences in maternal stress and mothering behaviors across stable family structures are explained by selection, with the exception that single mothers report higher levels of maternal stress. Mothers who experience instability over their child? s first year have similar levels of stress and behaviors as compared to mothers in stable relationships, with few exceptions.
Electrochemical detection of Pb and Cd in paper-based microfluidic devices

Filter paper strips combined with screen-printed carbon electrodes (SPCE) were used in the assembly of a microfluidic device for detecting Pb(II) and Cd(II) in aqueous samples. The portable microfluidic device (with direct electrochemical detection) was tested for direct quantification of multiple analytes (Pb(II) and Cd(II)) in contaminated aqueous samples without pretreatment. Square wave anodic stripping voltammetric (SWASV) signal was greatly enhanced and displayed excellent analytical performance for Pb(II) and Cd(II) detection (from 0 to 100 ppb) with low limit of detection of 2.0 and 2.3 ppb, respectively. The proposed electrochemical devices also exhibited good selectivity and stability for analyses of real samples of gas dissolved salty soda water and ground water with physical contamination. The device is simple, low-cost, easy-to-fabricate and portable. This analytical device has the possibility of being useful for point-of-care applications in environmental monitoring, public health and food safety.
The diagnostic accuracy of questions about past experiences of being mechanically restrained in a population of psychiatric patients

Information about stressful life experiences obtained from patients during diagnostic interviews is an important foundation for clinical decision making. In this study self-reports from 115 committed psychiatric patients of experiences of mechanical restraint were compared with medical records. The sensitivity of patient self-reports was 73% (11/15) and the specificity was 92% (92/100). No clear relationship between psychiatric symptoms and reliability of self-reports was identified. The results highlight the subjective qualities of narratives about past experiences.
Design and Evaluation on the Mobile Application of Transcutaneous Electrical Nerve Stimulation (TENS)

This study aims to design a transcutaneous electrical nerve stimulation Application (TENS App) according to the suggestions from potential users. To the best of our knowledge, this is the first App including meridian and acupoints for TENS. After its development, there are eight participants recruited for evaluating the usability. Despite two out of eight users reporting that the typical TENS system requires lower cost and has better functionality than TENS App, the results show that almost seventy percent of participants have a better perception of TENS App on price, functionality, convenience, operational ability, and quality. However, participants still reported concerns about the safety issue of adopting TENS App. Therefore, for people who are the first time or unfamiliar with TENS App, instructions from occupational or physical therapists are recommended. We conclude that by using TENS App, users can not only use the portable electrotherapy devices at anyplace, but also reduce their outpatient visits.
Cost data for individual patients included in clinical studies: no amount of statistical analysis can compensate for inadequate costing methods.

This work examines the quality of the cost methods used to derive patient level costs in 45 economic evaluations conducted alongside randomised controlled trials. The perspective of the cost analysis, the methods used to determine quantities and values of resources and how the cost data were reported are examined. The reported costing methods were found to be of poor quality, highlighting the need for greater rigour. Researchers to date appear more concerned with whether cost data have been subjected to the appropriate statistical analysis. For the results of clinical studies to be valid both cost methods and the methods used for the statistical analysis of cost data should be of a high quality.
An optimized conflict-free replicated set

Eventual consistency of replicated data supports concurrent updates, reduces latency and improves fault tolerance, but forgoes strong consistency. Accordingly, several cloud computing platforms implement eventually-consistent data types. The set is a widespread and useful abstraction, and many replicated set designs have been proposed. We present a reasoning abstraction, \emph{permutation equivalence}, that systematizes the characterization of the expected concurrency semantics of concurrent types. Under this framework we present one of the existing conflict-free replicated data types, Observed-Remove Set. Furthermore, in order to decrease the size of meta-data, we propose a new optimization to avoid tombstones. This approach that can be transposed to other data types, such as maps, graphs or sequences.
Clopidogrel hydrogen sulphate for atrial fibrillation

Introduction: Atrial fibrillation is a common cardiac rhythm abnormality with a considerable cardiovascular disease burden worldwide. It is an independent major risk factor for stroke. Stroke prevention with anticoagulation or antiplatelet agents has been an important area of clinical research. Warfarin is the most widely used antithrombotic therapy for stroke prophylaxis for last several years, and now dabigatran (150 mg b.i.d.) is more effective than warfarin in stroke prevention in individuals at increased of stroke. In addition, several studies have evaluated the efficacy of clopidogrel for stroke prophylaxis either alone or in combination with aspirin. Areas covered: This review summarizes the key findings of the trials looking at the efficacy of clopidogrel in stroke prevention. A literature search was performed using PubMed and Google Scholar. The trials that evaluated the efficacy of clopidogrel in preventing atherothrombotic events or stroke were also included. Expert opinion: Clopidogrel prevents more vascular events, including stroke, in patients with a recent myocardial infarction, stroke or peripheral vascular disease than aspirin. Combination of clopidogrel and aspirin provides a greater reduction of stroke than aspirin or clopidogrel monotherapy, but at an increased risk of bleeding. Dual antiplatelet therapy (clopidogrel and aspirin) is inferior to warfarin in primary stroke prevention for patient with atrial fibrillation and thus should be considered for stroke prophylaxis only in patients ineligible for warfarin. However, with the advent of newer agents, like direct thrombin inhibitors and Factor Xa inhibitors, the role of antiplatelet therapy for stroke prevention in atrial fibrillation remains unclear.
Diagnosis of Idiopathic Pulmonary Fibrosis “Pragmatic Challenges in Clinical Practice”

The past few years have signaled a major breakthrough on the management of idiopathic pulmonary fibrosis (IPF). Finally, we have drugs in our arsenal able to slow down the inexorable disease natural course. On the other hand, the latter evidence has increased the responsibility for a timely and accurate diagnosis. Establishment of IPF diagnosis directly affects the choice of appropriate treatment. The current diagnostic guidelines represent a major step forward providing an evidence-based road map; yet, clinicians are encountering major diagnostic dilemmas that inevitably affect therapeutic decisions. This review article aims to summarize the current state of knowledge on the diagnostic procedure of IPF based on the current guidelines and discuss pragmatic difficulties and challenges encountered by clinicians with regards to their applicability in the everyday clinical practice.
Communication that heals: mindful communication practices from palliative care leaders

Abstract Though research has begun to highlight the centrality of communication in palliative care, studies have yet to focus on the use of mindful communication. Mindful communication is associated with increases in patient care and decreases in physician burnout. Through in-depth, semi-structured interviews the authors sought mindful communication practices from palliative care leaders in American Hospital Association Circle of Life® award-wining units. Four key mindful communication practices emerged: Know your audience, ask questions, discard scripts, and recognize your role. The discussion articulates how key mindful communication practices may be used as a stage model, where key practices may be used individually or in concert, by sole practitioners or within interdisciplinary teams and by new and seasoned clinicians. Theoretical contributions and areas for future inquiry are also discussed.