Miracle-dz/UniFAR-FTUs huggingface dataset:
High-volume resistance training reduces postprandial lipaemia in postmenopausal women

Abstract The aim of this study was to compare the effects of 11 weeks of low-volume resistance training (LVRT) and high-volume resistance training (HVRT) on muscle strength, muscle thickness (MT), and postprandial lipaemia (PPL) in postmenopausal women. Thirty-six healthy and untrained postmenopausal women (age, 58.9 ± 5.8 years; 68.6 ± 10.3 kg; and BMI, 26.9 ± 4.8 kg · m−2) participated in resistance training 3× per week for 11 weeks (HVRT = 12; LVRT = 13; and control group = 11). Biochemical variables, both pretraining and post-training, were evaluated 16 h after the administration of an oral fat tolerance test (OFTT) and metabolic variable during [energy expenditure (EE)] and after training session [excess postexercise oxygen consumption (EPOC)]. Muscle strength (1 RM) and MT were also calculated, and no significant differences were observed between the groups for PPL (mmol · L−1 per 5 h) as measured by glucose, high-density lipoprotein, low-density lipoprotein, and total cholesterol. EE total (EE + EPOC; 6.12 ± 1.21 MJ vs. 2.26 ± 0.85 MJ), resting fat oxidation (5.52 ± 1.69 g · h−1 vs. 4.11 ± 1.12 g · h−1); MT (vastus medialis, 21.4 ± 1.8 mm vs. 18.4 ± 1.2 mm and vastus lateralis 22.3 ± 1.2 mm vs. 20.8 ± 1.3 mm); triacylglycerol (TAG) 0, 1, 2, 4; and 5 h after OFTT, TAG area under the curve (AUC) (5.79 ± 0.42 vs. 7.78 ± 0.68), and incremental AUC (−46.21 ± 14.42% vs. 7.78 ± 4.68%) were all significantly different post-training for HVRT versus LVRT, respectively (P < 0.05). The results of this investigation suggest that HVRT reduces PPL in postmenopausal women.
Relationship Between Perceived Stigma and Depression Severity

The purpose of this study was to explore the relationship between perceived stigma and being in treatment for depression and current depression severity. Face-to-face interviews were conducted with a convenience sample of depressed subjects from a Veterans Administration outpatient mental health clinic (N = 54) and never-depressed subjects from a Veterans Administration primary care clinic (N = 50). Depression severity was measured using the 9-item Primary Care Evaluation of Mental Disorders depression measure. Stigma was measured using the 5-item Stigma Scale for Receiving Psychological Help modified for depression treatment. Statistical analyses included Spearman correlation and multivariate regression. In the correlation analysis, being in treatment for depression compared with never experiencing depression was associated with significantly higher levels of perceived stigma (p < .001). In separate multivariate models controlling for significant univariate correlates, greater depression severity (p < .001) and meeting criteria for current major depression (p < .001) were significant predictors of perceived stigma. Greater depression severity appears to be a strong predictor of perceived stigma.
Longitudinal Use of a Line Probe Assay for Human Immunodeficiency Virus Type 1 Protease Predicts Phenotypic Resistance and Clinical Progression in Patients Failing Highly Active Antiretroviral Therapy

ABSTRACT An observational study assessed the longitudinal use of a new line probe assay for the detection of protease mutations. Probe assays for detection of reverse transcriptase (Inno-LiPA HIV-1 RT; Innogenetics) and protease (prototype kit Inno-LiPA HIV Protease; Innogenetics) mutations gave results for 177 of 199 sequential samples collected over 2 years from 26 patients failing two nucleoside reverse transcriptase inhibitors and one protease inhibitor (first line: indinavir, n = 6; ritonavir, n = 10; and saquinavir, n = 10). Results were compared to recombinant virus protease inhibitor susceptibility data (n = 87) and to clinical and virological data. Combinations of protease mutations (M46I, G48V, I54V, V82A or -F, I84V, and L90M) predicted phenotypic resistance to the protease inhibitor and to nelfinavir. The sum of protease mutations was associated with virological and clinical outcomes from 6 and 3 months on, respectively. Moreover, a poorer clinical outcome was linked to the sum of reverse transcriptase mutations. In conclusion, despite the limited number of patients studied and the restricted number of codons investigated, probe assay-based genotyping correlates with phenotypic drug resistance and predicts new Centers for Disease Control and Prevention stage B and C clinical events and virological outcome. Line probe assays provide additional prognostic information and should be prospectively investigated for their potential for treatment monitoring.
Capturing the Connections: Unboxing Internet of Things Devices

Based upon a study of how to capture data from Internet of Things (IoT) devices, this paper explores the challenges for data centric design ethnography. Often purchased to perform specific tasks, IoT devices exist in a complex ecosystem. This paper describes a study that used a variety of methods to capture the interactions an IoT device engaged in when it was first setup. The complexity of the study that is explored through the annotated documentation across video and router activity, presents the ethnographic challenges that designers face in an age of connected things.
Identifying and Classifying Small and Medium Sized Towns in Europe

This paper provides a first attempt at the construction of a unified, homogeneous inventory of different classes of urban settlements in the European space, building on the approach of international institutions such as OECD and the EU in relation to larger urban areas and extending it to the specific challenge presented by smaller settlements. Its objective is twofold. The first is to address the fundamental empirical problem that was central to the development of the ESPON 2013 project ‘Small and Medium sized Towns in their Functional Territorial Context’ (TOWN), that is the proper geographic identification of different classes of urban settlements. The second is to introduce one basic classification of urban settlements, and two more refined typologies of small and medium sized towns (SMST). These typologies are used to provide a first impression of territorial structures of urbanisation throughout Europe, further elaborated in functional terms in the TOWN project.
Evidence for progression of brain structural abnormalities in schizophrenia: beyond the neurodevelopmental model.

OBJECTIVE Clinical, neuroimaging, neuropathological and neuropsychological evidence suggests that, in schizophrenia, there is structural and functional disturbance of the hippocampus. The purpose of this paper is to present published findings concerning the nature, timing and course of these putative disturbances of hippocampal function and the pathophysiological mechanisms involved, and to explore whether schizophrenia is a disorder of neurodevelopment, neurodegeneration or a combination of both processes. METHOD The available cross-sectional and longitudinal evidence for hippocampal involvement in schizophrenia is reviewed and a model of hippocampal involvement in this disorder, which derives from our own cross-sectional and longitudinal hippocampal imaging data, is described. RESULTS We propose a three-hit model in which an early neurodevelopmental lesion renders the hippocampus vulnerable to further insult later in life during the transition phase to active illness. The available evidence suggests that the left hippocampus is particularly vulnerable during these early stages, while further insult involving the hippocampus bilaterally occurs in those who develop a chronic form of the illness. CONCLUSIONS Intervention strategies should target the most vulnerable stages of the illness, in particular the transition phase to psychosis, when novel treatments may prevent the illness or ameliorate its effects.
From Saccades to Grasping: A Model of Coordinated Reaching Through Simulated Development on a Humanoid Robot

Infants demonstrate remarkable talents in learning to control their sensory and motor systems. In particular the ability to reach to objects using visual feedback requires overcoming several issues related to coordination, spatial transformations, redundancy, and complex learning spaces. This paper describes a model of longitudinal development that covers the full sequence from blind motor babbling to successful grasping of seen objects. This includes the learning of saccade control, gaze control, torso control, and visually-elicited reaching and grasping in 3-D space. This paper builds on and extends our prior investigations into the development of gaze control, eye-hand coordination, the use of constraints to shape learning, and a schema memory system for the learning of sensorimotor experience. New contributions include our application of the LWPR algorithm to learn how movements of the torso affect the robot's representation of space, and the first use of the schema framework to enable grasping and interaction with objects. The results from our integration of these various components into an implementation of longitudinal development on an iCub robot show their ability to generate infant-like development, from a start point with zero coordination up to skilled spatial reaching in less than three hours.
Using learning automata to determine proper subset size in high-dimensional spaces

Abstract In this paper, we offer a new method called FSLA (Finding the best candidate Subset using Learning Automata), which combines the filter and wrapper approaches for feature selection in high-dimensional spaces. Considering the difficulties of dimension reduction in high-dimensional spaces, FSLA’s multi-objective functionality is to determine, in an efficient manner, a feature subset that leads to an appropriate tradeoff between the learning algorithm’s accuracy and efficiency. First, using an existing weighting function, the feature list is sorted and selected subsets of the list of different sizes are considered. Then, a learning automaton verifies the performance of each subset when it is used as the input space of the learning algorithm and estimates its fitness upon the algorithm’s accuracy and the subset size, which determines the algorithm’s efficiency. Finally, FSLA introduces the fittest subset as the best choice. We tested FSLA in the framework of text classification. The results confirm its promising performance of attaining the identified goal.
Identification of a BMP‐responsive element in Id1, the gene for inhibition of myogenesis

Background: Bone morphogenetic protein‐2 (BMP‐2) stimulates osteoblast differentiation, but inhibits myogenic differentiation in C2C12 myoblasts. BMP‐2 induces transcription of Id1, an inhibitor for myogenesis, within 1 h in the cells. To examine the molecular mechanism of the action of BMP‐2, we analysed a BMP‐2‐responsive element (BRE) in the 5′ flanking region of the human Id1 gene.
Structure and genetic relationship of five populations from central India based on 15 autosomal STR loci

Abstract Aim: To estimate population parameters based on allele frequencies obtained for 15 polymorphic autosomal STR loci investigated in caste and tribal populations of central India (n = 419). Methods: Multiplexed PCR amplifications of the 15 Autosomal STR Loci were performed and amplified products were genotyped using multi-capillary electrophoresis on an ABI 3100 genetic analyser. Parameters of population genetics and forensic interest based on the allele frequencies were calculated. Genetic affinity of the studied populations among themselves and with previously reported populations of India was also analysed using distance-based NJ tree and using PCA plot. Results: All the 15 STR loci were highly informative and discriminating, with CPD of 0.999 99. Except for Brahmins and Rajput, all other studied populations were in Hardy–Weinberg equilibrium (HWE). The only tribe (Gond) population studied showed significant variation with the other four caste populations (Brahmin, Yadav, Rajput and Muslim) studied and formed a cluster with other previously reported tribal populations of India. Nei’s genetic distance based clustering pattern of the NJ tree and the PCA plot showed the same pattern of genetic relationship, i.e. caste and tribal populations formed a distinct cluster. Conclusions: With respect to the distribution of alleles at each STR locus, the studied loci were found to be substantially polymorphic in all the studied populations, indicating good informativeness of all 15 STR markers. The population data generated in this study are useful for forensic, anthropological and demographic studies.
Performance evaluation of solid state fault current limiters in electric distribution system

The expansion of electric distribution system has resulted in an increasing need of fault current limiter in so as to limit fault currents to the downstream protection devices as a means of improving power quality. Considerations in the use of fault current limiter are that it must be reliable in operation, compact and inexpensive. This paper presents a comprehensive performance evaluation on the use of solid-state fault current limiters (FCL) in a distribution system. The FCL simulation model has been developed using the electromagnetic transient program PSCAD/EMTDC and a new control strategy for the FCL has been proposed for instantaneous detection of over current. The FCL is evaluated in terms of its performance in limiting fault current to the tolerable limit of the system, as well as the response of the FCL within a fraction of a cycle of about 2 ms. The effects of varying circuit parameters such as R, L, and C as part of the FCL device has been investigated. It has been noted that the inductive reactor, which is part of the FCL device, is suitable for limiting the fault current. The impact of induction motor loads on the FCL has also been analyzed. Simulation results show that the FCL is effective for reducing short circuit currents and also can be used to protect bus bars from voltage sag when the system is subjected to various types of faults.
Association between peroxisome proliferator-activated receptor-γ gene polymorphism (Pro12Ala) and Helicobacter pylori infection in gastric carcinogenesis

Abstract Objective. Helicobacter pylori infection is accompanied by inflammatory processes leading to peptic ulcer and gastric cancer in the minority of infected individuals. The interaction between H. pylori virulence factors, host defense mechanisms and environmental factors determine the outcome of clinical manifestations. One of the host factors involved in the processes of inflammation and carcinogenesis is the peroxisome proliferator-activated receptor-γ (PPAR-γ) molecule. The present case–control study aimed to determine polymorphism of PPAR-γ gene and its association with H. pylori infection and gastrointestinal diseases (peptic ulcer and non-cardia gastric cancer) in Iranian patients. Materials and methods. One hundred and fifty-five patients with upper gastrointestinal diseases (76 peptic ulcer and 79 non-cardia gastric cancer) and 152 matched controls were genotyped for PPAR-γ gene polymorphism (Pro12Ala) by the PCR–RFLP method. Infection with H. pylori was confirmed by histology, the rapid urease test (RUT) and ELISA assay (IgG anti-H. pylori). Results. The frequency of PPAR-γ G (Ala 12) allele was significantly higher in H. pylori positive patients with non-cardia gastric cancer than in controls (22.8% vs. 3.9%, p = 0.027; OR = 3.28; 95% CI = 1.21–8.89), But there was no significant difference without infection (p = 0.7). Moreover, the PPAR-γ polymorphism was not associated with peptic ulcer in the presence or absence of H. pylori infection. Conclusion. Our results indicated PPAR-γ G allele may be an important contributor to non-cardia gastric cancer in Iranian H. pylori infected patients.
Genotyping and subtyping of Giardia and Cryptosporidium isolates from commensal rodents in China

SUMMARY Cryptosporidium and Giardia are two important zoonotic intestinal parasites responsible for diarrhoea in humans and other animals worldwide. Rodents, as reservoirs or carriers of Cryptosporidium and Giardia, are abundant and globally widespread. In the present study, we collected 232 fecal specimens from commensal rodents captured in animal farms and farm neighbourhoods in China. We collected 33 Asian house rats, 168 brown rats and 31 house mice. 6·0% (14/232) and 8·2% (19/232) of these rodents were microscopy-positive for Giardia cysts and Cryptosporidium oocysts, respectively. All 14 Giardia isolates were identified as Giardia duodenalis assemblage G at a minimum of one or maximum of three gene loci (tpi, gdh and bg). By small subunit rRNA (SSU rRNA) gene sequencing, Cryptosporidium parvum (n = 12) and Cryptosporidium muris (n = 7) were identified. The gp60 gene encoding the 60-kDa glycoprotein was successfully amplified and sequenced in nine C. parvum isolates, all of which belonged to the IIdA15G1 subtype. Observation of the same IIdA15G1 subtype in humans (previously) and in rodents (here) suggests that rodents infected with Cryptosporidium have the potential to transmit cryptosporidiosis to humans.
Identification and Characterization of a Compound That Protects Cardiac Tissue from Human Ether-à-go-go-related Gene (hERG)-related Drug-induced Arrhythmias*

Background: Inhibition of the cardiac hERG channel by essential pharmaceuticals is unpredictable and leads to fatal arrhythmias. Results: Pretreatment with a newly identified compound, VU0405601, reduces sensitivity of hERG to inhibition by multiple blockers and prevents arrhythmias. Conclusion: hERG-related arrhythmias are amenable to preventive therapy. Significance: A novel approach at ion channel modulation that impacts drug discovery and safety concerns is outlined. The human Ether-à-go-go-related gene (hERG)-encoded K+ current, IKr is essential for cardiac repolarization but is also a source of cardiotoxicity because unintended hERG inhibition by diverse pharmaceuticals can cause arrhythmias and sudden cardiac death. We hypothesized that a small molecule that diminishes IKr block by a known hERG antagonist would constitute a first step toward preventing hERG-related arrhythmias and facilitating drug discovery. Using a high-throughput assay, we screened a library of compounds for agents that increase the IC70 of dofetilide, a well characterized hERG blocker. One compound, VU0405601, with the desired activity was further characterized. In isolated, Langendorff-perfused rabbit hearts, optical mapping revealed that dofetilide-induced arrhythmias were reduced after pretreatment with VU0405601. Patch clamp analysis in stable hERG-HEK cells showed effects on current amplitude, inactivation, and deactivation. VU0405601 increased the IC50 of dofetilide from 38.7 to 76.3 nm. VU0405601 mitigates the effects of hERG blockers from the extracellular aspect primarily by reducing inactivation, whereas most clinically relevant hERG inhibitors act at an inner pore site. Structure-activity relationships surrounding VU0405601 identified a 3-pyridiyl and a naphthyridine ring system as key structural components important for preventing hERG inhibition by multiple inhibitors. These findings indicate that small molecules can be designed to reduce the sensitivity of hERG to inhibitors.
Distribution of uidA gene sequences in Escherichia coli isolates in water sources and comparison with the expression of beta-glucuronidase activity in 4-methylumbelliferyl-beta-D-glucuronide media

The uidA gene, which encodes the beta-glucuronidase enzyme, was detected in 97.7% of 435 Escherichia coli isolates from treated and raw water sources by DNA-DNA hybridization; 92.4% of the strains expressed the translational product in 4-methylumbelliferyl-beta-D-glucuronide-containing media after reinoculation. Upon initial isolation from water samples, the minimal medium o-nitrophenyl-beta-D-galactopyranoside-4-methylum-belliferyl -beta-D-glucuronide preparations failed to detect more than 50% of the E. coli isolates that possessed uidA gene. Treated water gave the lowest recovery, with Colilert producing 26% positive samples and Coliquik producing 48% positive samples. There appears to be no relationship between the intensity of the autoradiographic signals of the uidA gene and the expression of beta-glucuronidase activity. Therefore, another variable such as physiological condition of the bacteria could be responsible for the nonexpression of the enzyme activity.
Developmental changes of serotonin 4(a) receptor expression in the rat pre‐Bötzinger complex

Serotonin receptors (5‐HTRs) are known to be involved in the regulation of breathing behavior and to mediate neurotrophic actions that exert a significant function in network formation during development. We studied neuronal 5‐HT4(a)R‐immunoreactivity (‐IR) at developmental ages from E14 to P10. Within the pre‐Bötzinger complex (pre‐BötC), a part of the respiratory network important for rhythmogenesis, 5‐HT4(a)R‐IR was most extensive in rats at an age of E18. The 5‐HT4(a)‐IR was found predominantly in the neuropil, whereas somatic staining was sporadic at late embryonic (E18–E20) stages. At birth, we observed a dramatic change to a predominantly somatic staining, and neuropil staining was greatly reduced and disappeared at an age of P4. In all developmental stages, 5‐HT4(a) and μ‐opioid receptors were strongly coexpressed in neurons of the pre‐BötC, whereas 5‐HT4(a)R expression was absent in neurons within the dorsal horn. Nestin, a marker for CNS progenitor cells, was used to obtain information about the degree of pre‐BötC differentiation. Nestin‐positive cells did not appear within the pre‐BötC before age E20. At E16, nestin‐expressing cells were absent in the nucleus ambiguus (NA) and its ventral periphery. The number of nestin‐positive cells increased after birth within and outside the pre‐BötC, the majority of cells being glial. Coexpression of nestin and 5‐HT4(a)R was localized predominantly within the NA and appeared only sporadically within the pre‐BötC. We conclude that 5‐HT4(a)Rs are important not only for neuromodulation of cellular excitability but also for respiratory network formation. J. Comp. Neurol. 506:775–790, 2008. © 2007 Wiley‐Liss, Inc.