Miracle-dz/UniFAR-FTUs huggingface dataset:
Near-Absent Levels of Segregational Variation Suggest Limited Opportunities for the Introduction of Genetic Variation Via Homeologous Chromosome Pairing in Synthetic Neoallotetraploid Mimulus

Genetic variation is the fundamental medium of evolution. In allopolyploids, which are the product of hybridization and whole genome duplication, if homologous chromosomes always pair, then all descendants of a single diploid F1 hybrid lineage will be genetically identical. Contrarily, genetic variation among initially isogenic lineages is augmented when homeologous chromosomes pair; this added variation may contribute to phenotypic evolution. Mimulus sookensis is a naturally occurring, small-flowered allotetraploid derived from the large-flowered Mimulus guttatus and small-flowered Mimulus nasutus. Because diploid F1 hybrids between M. guttatus and M. nasutus have large flowers, phenotypic evolution post-polyploidization is implied in M. sookensis. Here, we present genetic and phenotypic analyses of synthetic neoallotetraploid Mimulus derived from a cross between M. guttatus and M. nasutus. Genetic marker data from S2 and BC1N progeny suggest that chromosomes regularly pair with their homologous counterpart. By measuring the phenotype of synthetic neoallotetraploids, we demonstrate that polyploidization per se does not induce the small flowers of M. sookensis. Moreover, phenotypic measurements of synthetic allotetraploid F2s and S4 families suggest that rare homeologous recombination events have a negligible phenotypic effect in the first few generations. In total, the results are consistent with either exceedingly rare homeologous pairing and recombination or spontaneous fragment loss. The low levels of fragment loss and phenotypic variation in neoallotetraploids suggest that homeologous recombination after polyploidization is not a major mechanism of phenotypic evolution in M. sookensis. Rather, it may be that spontaneous mutations or epigenetic changes after allopolyploidization have driven phenotypic evolution in M. sookensis.
Enhanced Fisher linear discriminant models for face recognition

We introduce two enhanced Fisher linear discriminant (FLD) models (EFM) in order to improve the generalization ability of the standard FLD based classifiers such as Fisherfaces. Similar to Fisherfaces, both EFM models apply first principal component analysis (PCA) for dimensionality reduction before proceeding with FLD type of analysis. EFM-1 implements the dimensionality reduction with the goal to balance between the need that the selected eigenvalues account for most of the spectral energy of the raw data and the requirement that the eigenvalues of the within-class scatter matrix in the reduced PCA subspace are not too small. EFM-2 implements the dimensionality reduction as Fisherfaces do. It proceeds with the whitening of the within-class scatter matrix in the reduced PCA subspace and then chooses a small set of features (corresponding to the eigenvectors of the within-class scatter matrix) so that the smaller trailing eigenvalues are not included in further computation of the between-class scatter matrix. Experimental data using a large set of faces-1,107 images drawn from 369 subjects and including duplicates acquired at a later time under different illumination-from the FERET database shows that the EFM models outperform the standard FLD based methods.
Onjisaponin B Derived from Radix Polygalae Enhances Autophagy and Accelerates the Degradation of Mutant α-Synuclein and Huntingtin in PC-12 Cells

Emerging evidence indicates important protective roles being played by autophagy in neurodegenerative disorders through clearance of aggregate-prone or mutant proteins. In the current study, we aimed to identify autophagy inducers from Chinese medicinal herbs as a potential neuroprotective agent that enhances the clearance of mutant huntingtin and α-synuclein in PC-12 cells. Through intensive screening using the green fluorescent protein-light chain 3 (GFP-LC3) autophagy detection platform, we found that the ethanol extracts of Radix Polygalae (Yuan Zhi) were capable of inducing autophagy. Further investigation showed that among three single components derived from Radix Polygalae—i.e., polygalacic acid, senegenin and onjisaponin B—onjisaponin B was able to induce autophagy and accelerate both the removal of mutant huntingtin and A53T α-synuclein, which are highly associated with Huntington disease and Parkinson disease, respectively. Our study further demonstrated that onjisaponin B induces autophagy via the AMPK-mTOR signaling pathway. Therefore, findings in the current study provide detailed insights into the protective mechanism of a novel autophagy inducer, which is valuable for further investigation as a new candidate agent for modulating neurodegenerative disorders through the reduction of toxicity and clearance of mutant proteins in the cellular level.
Sub-MICs of cefuroxime and ciprofloxacin influence interaction of complement and immunoglobulins with Klebsiella pneumoniae

Growth of encapsulated (K+) and nonencapsulated (K-) Klebsiella pneumoniae strains in media containing sub-MICs of either cefuroxime or ciprofloxacin resulted in cell elongation but had little effect on the outer membrane protein or lipopolysaccharide profiles. Exposure to serum complement increased the surface hydrophobicity of a K- strain but failed to interact or to increase the surface hydrophobicity of the K+ strains. However, after growth of the K+ strains in sub-MICs of the antibiotics, complement increased their surface hydrophobicity and complement C3 was detected bound to their surface. Antisera raised against a K-O- strain agglutinated the K+ strains grown in the presence but not in the absence of cefuroxime or ciprofloxacin. These findings suggest that the filamentous morphology induced by these antibiotics influences the distribution or amount of capsular polysaccharide such that cell envelope components previously masked by the capsule become accessible to complement and immunoglobulins.
Meristem‐specific expression of epigenetic regulators safeguards transposon silencing in Arabidopsis

In plants, transposable elements (TEs) are kept inactive by transcriptional gene silencing (TGS). TGS is established and perpetuated by RNA‐directed DNA methylation (RdDM) and maintenance methylation pathways, respectively. Here, we describe a novel RdDM function specific for shoot apical meristems that reinforces silencing of TEs during early vegetative growth. In meristems, RdDM counteracts drug‐induced interference with TGS maintenance and consequently prevents TE activation. Simultaneous disturbance of both TGS pathways leads to transcriptionally active states of repetitive sequences that are inherited by somatic tissues and partially by the progeny. This apical meristem‐specific mechanism is mediated by increased levels of TGS factors and provides a checkpoint for correct epigenetic inheritance during the transition from vegetative to reproductive phase and to the next generation.
The Pronounced Th17 Profile in Systemic Sclerosis (SSc) Together with Intracellular Expression of TGFβ and IFNγ Distinguishes SSc Phenotypes

Background Systemic sclerosis (SSc) is an autoimmune disease where controversy on Th1/Th2 balance dominates. We investigated whether the recently discovered Th17 pattern was present in SSc. Methodology and Principal Findings Patients were subdivided as having limited cutaneous SSc (lcSSc, n = 12) or diffuse cutaneous SSc (dcSSc, n = 24). A further arbitrary subdivision was made between early dcSSc (n = 11) and late dcSSc (n = 13) based upon the duration of disease. As a comparator group 14 healthy controls were studied. CD3+ cells were isolated using FACS and subsequently studied for the expression of CD4, CD8, CD25, CD45Ro, CD45Ra, IL-23, GITR, CD69 and intracellular expression of IL-17, TGFβ and IFNγ using flow cytometry. Levels of IL-17, IL-6, IL-1α and IL-23 were measured using Bioplex assays. SSc patients had more and more activated CD4+ cells. In addition, CD4, CD45Ro and CD45Ra cells from all SSc patients highly expressed the IL23R, which was associated with a higher IL-17 expression as well. In contrast, IFNγ and TGFβ were selectively up regulated in SSc subsets. In line with these observation, circulating levels of IL-17 inducing cytokines IL-6, IL-23 and IL-1α were increased in all or subsets of SSc patients. Conclusion and Significance The combination of IL-17, IFNγ and TGFβ levels in CD45Ro and CD45Ra cells from SSc patients is useful to distinguish between lSSc, ldSSc or edSSc. Blocking Th17 inducing cytokines such as IL-6 and IL-23 may provide a useful tool to intervene in the progression of SSc.
Children, but not chimpanzees, have facial correlates of determination

Facial expressions have long been proposed to be important agents in forming and maintaining cooperative interactions in social groups. Human beings are inordinately cooperative when compared with their closest-living relatives, the great apes, and hence one might expect species differences in facial expressivity in contexts in which cooperation could be advantageous. Here, human children and chimpanzees were given an identical task designed to induce an element of frustration (it was impossible to solve). In children, but not chimpanzees, facial expressions associated with effort and determination positively correlated with persistence at the task. By contrast, bodily indicators of stress (self-directed behaviour) negatively correlated with task persistence in chimpanzees. Thus, children exhibited more behaviour as they persisted, and chimpanzees exhibited less. The facial expressions produced by children, could, therefore, function to solicit prosocial assistance from others.
Manet Load Balancing Parallel Routing Protocol

In recent years, multi-path routing protocols have attained more attention in mobile ad hoc networks as compared to other routing schemes due to their abilities and efficiency in improving bandwidth of communication, increasing delivery reliability, responding to congestion and heavy traffic. Several protocols have been developed to address multi path routing, but it always has a problem that the discovered paths may be not 100% disjoint and sending data is done in only one path until it's broken; the discovery of multiple paths also generates more overhead on the network. Load Balancing Parallel Routing Protocol [LBPRP] tried to solve previous multi path problems, distributing traffic among multiple paths) sending data in parallel form as it uses all paths in the same time. We employed a simple test scenario to be sure of proposed model efficiency and to validate the proposed Load Balancing Parallel Routing Protocol. [LBPRP] will achieve load balancing in sending data, decreasing the end-to-end delay and increasing the packet delivery ratio and throughput, thus the performance of multi-path routing protocols can be improved consequently.
β‐amyloid and postural instability and gait difficulty in Parkinson's disease at risk for dementia

Although motor impairments in Parkinson's disease (PD) are attributed to nigrostriatal dopaminergic denervation, postural instability and gait difficulty (PIGD) features are less responsive to dopaminergic medications. PIGD features are a risk factor also for the development of dementia in PD (PDD). These observations suggest that nondopaminergic mechanisms may contribute to axial motor impairments. The aim was to perform a correlative PET study to examine the relationship between neocortical β‐amyloid deposition ([11C]‐Pittsburgh Compound B), nigrostriatal dopaminergic denervation ([11C]‐dihydrotetrabenazine), and PIGD feature severity in PD patients at risk for dementia. This was a cross‐sectional study of 44 PD patients (11 female and 33 male; 69.5 ± 6.6 years of age; 7.0 ± 4.8 years motor disease duration; mean H & Y stage: 2.7 ± 0.5) who underwent PET, motor feature severity assessment using the Movement Disorder Society revised UPDRS, and the Dementia Rating Scale (DRS). Linear regression (R2adj = 0.147; F4,39 = 2.85; P = 0.036) showed that increased PIGD feature severity was associated with increased neocortical [11C]‐Pittsburgh Compound B binding (β = 0.346; t39 = 2.13; P = 0.039) while controlling for striatal [11C]‐dihydrotetrabenazine binding, age, and DRS total score. Increased neocortical β‐amyloid deposition, even at low‐range levels, is associated with higher PIGD feature severity in PD patients at risk for dementia. This finding may explain why the PIGD motor phenotype is a risk factor for the development of PDD. © 2012 Movement Disorder Society
Steganography in Modern Smartphones and Mitigation Techniques

By offering sophisticated services and centralizing a huge volume of personal data, modern smartphones changed the way we socialize, entertain and work. To this aim, they rely upon complex hardware/software frameworks leading to a number of vulnerabilities, attacks and hazards to profile individuals or gather sensitive information. However, the majority of works evaluating the security degree of smartphones neglects steganography, which can be mainly used to: i) exfiltrate confidential data via camouflage methods, and ii) conceal valuable or personal information into innocent looking carriers. Therefore, this paper surveys the state of the art of steganographic techniques for smartphones, with emphasis on methods developed over the period 2005 to the second quarter of 2014. The different approaches are grouped according to the portion of the device used to hide information, leading to three different covert channels, i.e., local, object and network. Also, it reviews the relevant approaches used to detect and mitigate steganographic attacks or threats. Lastly, it showcases the most popular software applications to embed secret data into carriers, as well as possible future directions.
Does intrinsic motivation enhance motor cortex excitability?

Intrinsic motivation (IM) is often viewed as a spontaneous tendency for action. Recent behavioral and neuroimaging evidence indicate that IM, in comparison to extrinsic motivation (EM), solicits the motor system. Accordingly, we tested whether IM leads to greater excitability of the motor cortex than EM. To test this hypothesis, we used two different tasks to induce the motivational orientation using either words representing each motivational orientation or pictures previously linked to each motivational orientation through associative learning. Single-pulse transcranial magnetic stimulation over the motor cortex was applied when viewing the stimuli. Electromyographic activity was recorded on the contracted first dorsal interosseous muscle. Two indexes of corticospinal excitability (the amplitude of motor-evoked potential and the length of cortical silent period) were obtained through unbiased automatic detection and analyzed using a mixed model that provided both statistical power and a high level of control over all important individual, task, and stimuli characteristics. Across the two tasks and the two indices of corticospinal excitability, the exposure to IM-related stimuli did not lead to a greater corticospinal excitability than EM-related stimuli or than stimuli with no motivational valence (ps > .20). While these results tend to dismiss the advantage of IM at activating the motor cortex, we suggest alternative hypotheses to explain this lack of effect, which deserves further research.
A Framework for End-to-End Evaluation of 5G mmWave Cellular Networks in ns-3

The growing demand for ubiquitous mobile data services along with the scarcity of spectrum in the sub-6 GHz bands has given rise to the recent interest in developing wireless systems that can exploit the large amount of spectrum available in the millimeter wave (mmWave) frequency range. Due to its potential for multi-gigabit and ultra-low latency links, mmWave technology is expected to play a central role in 5th Generation (5G) cellular networks. Overcoming the poor radio propagation and sensitivity to blockages at higher frequencies presents major challenges, which is why much of the current research is focused at the physical layer. However, innovations will be required at all layers of the protocol stack to effectively utilize the large air link capacity and provide the end-to-end performance required by future networks. Discrete-event network simulation will be an invaluable tool for researchers to evaluate novel 5G protocols and systems from an end-to-end perspective. In this work, we present the first-of-its-kind, open-source framework for modeling mmWave cellular networks in the ns-3 simulator. Channel models are provided along with a configurable physical and MAC-layer implementation, which can be interfaced with the higher-layer protocols and core network model from the ns-3 LTE module to simulate end-to-end connectivity. The framework is demonstrated through several example simulations showing the performance of our custo mmmWave stack.
In-Memory Business Process Management

In-memory databases have become a mainstay of enterprise computing offering significant performance boosts for OLAP and OLTP workloads as well as improved prospects for application integration through an efficient, shared database layer. Despite significant R&D investments into in-memory data management, limited insights are available on the impacts of middleware platforms for application integration, i.e., How they need to evolve to leverage in-memory database capabilities. This paper provides a first exposition into how in-memory databases impact Business Process Management, as a mission-critical model-driven application integration middleware. Through it, we discuss how in-memory databases will render some prevalent uses cases of BPM middleware obsolete, while opening up prospects for tighter application integration, better process automation performance and some entirely new BPM capabilities such as process-based application customization. To validate the feasibility of an in-memory BPM, we develop a surprisingly simple BPM runtime embedded into SAP HANA and providing for BPMN-based process automation capabilities.
Association between Hepatocyte Growth Factor (HGF) Gene Polymorphisms and Serum HGF Levels in Patients with Rheumatoid Arthritis.

OBJECTIVE Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by proliferation and insufficient apoptosis of synovial cell, inflammatory cell infiltration, angiogenesis, and destruction of joints. Hepatocyte growth factor (HGF) has many functions, such as regulation of inflammation, angiogenesis, and inhibition of apoptosis. The purpose of this study was to investigate the association between intron 13 C/A and intron 14 T/C HGF gene polymorphisms and serum HGF levels in patients with RA. MATERIALS AND METHODS 100 patients with RA and 123 healthy controls were included in this study. Serum HGF concentrations were measured using ELISA kit. Gene polymorphisms were determined by allelic discrimination analysis using the real-time PCR method. RESULTS HGF levels, frequency of AA genotype and A allele for intron 13 C/A polymorphism and frequency of CC genotype and C allele for intron 14 T/C polymorphism were increased in patients with RA compared to healthy controls. There was no overall associations between genotypes and serum HGF concentrations in both patient and control groups. CONCLUSION Our results indicate that HGF protein and gene may play an important role in the etiopathogenesis of RA. However, further studies are required for a better understanding of mechanisms related to the disease process.
Structure/function analysis of spinalin, a spine protein of Hydra nematocysts

The nematocyst capsules of the cnidarians are specialized explosive organelles that withstand high osmotic pressures of ≈ 15 MPa (150 bar). A tight disulfide network involving cysteine‐rich capsule wall proteins, like minicollagens and nematocyst outer wall antigen, characterizes their molecular composition. Nematocyst discharge leads to the expulsion of a long inverted tubule that was coiled inside the capsule matrix before activation. Spinalin has been characterized as a glycine‐rich, histidine‐rich protein associated with spine structures on the surface of everted tubules. Here, we show that full‐length Hydra spinalin can be expressed recombinantly in HEK293 cells and has the property to form disulfide‐linked oligomers, reflecting its state in mature capsules. Furthermore, spinalin showed a high tendency to associate into dimers in vitro and in vivo. Our data, which show incomplete disulfide connectivity in recombinant spinalin, suggest a possible mechanism by which the spine structure may be linked to the overall capsule polymer.
Enhancing coexistence in the unlicensed band with massive MIMO

We consider cellular base stations (BSs) equipped with a large number of antennas and operating in the unlicensed band. We denote such system as massive MIMO unlicensed (mMIMO-U). We design the key procedures required to guarantee coexistence between a cellular BS and nearby Wi-Fi devices. These include: neighboring Wi-Fi channel covariance estimation, allocation of spatial degrees of freedom for interference suppression, and enhanced channel sensing and data transmission phases. We evaluate the performance of the so-designed mMIMO-U, showing that it allows simultaneous cellular and Wi-Fi transmissions by keeping their mutual interference below the regulatory threshold. The same is not true for conventional listen-before-talk (LBT) operations. As a result, mMIMO-U boosts the aggregate cellular-plus-Wi-Fi data rate in the unlicensed band with respect to conventional LBT, exhibiting increasing gains as the number of BS antennas grows.
A bio-inspired, active morphing skin for camber morphing structures

In this study, one kind of developed morphing skin embedded with pneumatic muscle fibers (PMFs) was manufactured and was employed for camber morphing structures. The output force and contraction of PMF as well as the morphing skin were experimentally characterized at a series of discrete actuator pressures varying from 0.15 to 0.35 MPa. The active morphing skin test results show that the output force is 73.59 N and the contraction is 0.097 (9.7%) at 0.35 MPa. Due to these properties, this active morphing skin could be easily used for the morphing structures. Then the proper airfoil profile was chosen to manufacture the adaptive airfoil in this study. The chord-wise bending airfoil structure was achieved by employing this kind of active morphing skin. Finally the deformed shapes of this chord-wise bending airfoil structure were obtained by 3-dimensions scanning measurement. Meanwhile the camber morphing structures were analyzed through the finite element method (FEM) and the deformed shapes of the upper surface skins were obtained. The experimental result and FEM analysis result of deformed shapes of the upper surface skins were compared in this paper.
Robust Speaker Recognition with Combined Use of Acoustic and Throat Microphone Speech

Accuracy of automatic speaker recognition (ASV) systems degrades severely in the presence of background noise. In this paper, we study the use of additional side information provided by a body-conducted sensor, throat microphone. Throat microphone signal is much less affected by background noise in comparison to acoustic microphone signal. This makes throat microphones potentially useful for feature extraction or speech activity detection. This paper, firstly, proposes a new prototype system for simultaneous data-acquisition of acoustic and throat microphone signals. Secondly, we study the use of this additional information for both speech activity detection, feature extraction and fusion of the acoustic and throat microphone signals. We collect a pilot database consisting of 38 subjects including both clean and noisy sessions. We carry out speaker verification experiments using Gaussian mixture model with universal background model (GMM-UBM) and i-vector based system. We have achieved considerable improvement in recognition accuracy even in highly degraded conditions.