Miracle-dz/UniFAR-FTUs huggingface dataset:
β‐tubulin cofactor D and ARL2 take part in apical junctional complex disassembly and abrogate epithelial structure

In epithelial cells, the apical junctional complex (AJC), composed of tight junctions (TJs) and adherens junctions (AJs), maintains cell‐surface polarity by forming a fence that prevents lateral movement and diffusion of proteins and lipids between the apical and basolateral PM and holds the epithelial monolayer intact through cell‐cell contacts. Disassembly of this complex is a prime event in development and cell transformation. Maintenance of the AJC has been shown to involve mainly the actin cytoskeleton. Recent findings also point to the involvement of the microtubule (MT) system. Here we show the first evidence that in polarized epithelial MDCK cells, ARF‐like protein 2 (ARL2) and β‐tubulin cofactor D, known to be involved in MT dynamics, have a role in disassembly of the AJC followed by cell dissociation from the epithelial mono‐layer, which is not dependent on MT depolymerization. In addition, we show that β‐tubulin cofactor D is partially localized to the lateral PM through its 15 C‐terminal amino acids and intact MTs. ARL2 inhibited β ‐tubulin cofactor D‐dependent cell dissociation from the monolayer and AJC disassembly. To our knowledge, this is the first evidence that β‐tubulin cofactor D plays a role in cells independent of its presumed role in folding tubulin heterodimers. We conclude that ARL2 and β ‐tubulin cofactor D participate in AJC disassembly and epithelial depolarization. — Shultz, T. , Shmuel, M. , Hyman, T. , and Altschuler, Y. β‐tubulin cofactor D and ARL2 take part in apical junctional complex disassembly and abrogate epithelial structure. FASEB J. 22, 168–182 (2008)
Potential for immunotherapy in soft tissue sarcoma

Soft tissue sarcomas (STS) are rare, heterogeneous tumors of mesenchymal origin. Despite optimal treatment, a large proportion of patients will develop recurrent and metastatic disease. For these patients, current treatment options are quite limited. Significant progress has been made recently in the use of immunotherapy for the treatment of other solid tumors (e.g. prostate cancer, melanoma). There is a strong rationale for immunotherapy in STS, based on an understanding of disease biology. For example, STS frequently have chromosomal translocations which result in unique fusion proteins and specific subtypes have been shown to express cancer testis antigens. In this review, we discuss the current status of immunotherapy in STS, including data from human studies with cancer vaccines, adoptive cell therapy, and immune checkpoint blockade. Further research into STS immunology is needed to help design logical, subtype-specific immunotherapeutic strategies.
End-to-end simulations of the E-ELT/METIS coronagraphs

The direct detection of low-mass planets in the habitable zone of nearby stars is an important science case for future E-ELT instruments such as the mid-infrared imager and spectrograph METIS, which features vortex phase masks and apodizing phase plates (APP) in its baseline design. In this work, we present end-to-end performance simulations, using Fourier propagation, of several METIS coronagraphic modes, including focal-plane vortex phase masks and pupil-plane apodizing phase plates, for the centrally obscured, segmented E-ELT pupil. The atmosphere and the AO contributions are taken into account. Hybrid coronagraphs combining the advantages of vortex phase masks and APPs are considered to improve the METIS coronagraphic performance.
Serologic and molecular characterization of the B(A) blood group in the Chinese population

Abstract B(A) phenotype individuals have normal B antigen and a small amount of A antigen on the RBCs with anti-A in the plasma. Some highly potent monoclonal anti-A reagents are capable of agglutinating B(A) RBCs, which therefore usually results in a discrepancy between RBC and plasma ABO grouping. To date, five B(A) alleles (ABO*B(A)01, B(A)02, B(A)03, B(A)04, and B(A)05) have been defined by nucleotide sequences. To get a more complete picture of B(A) phenotypes found in the Chinese population and resolve blood donor typing problems caused by B(A) alleles, a serologic and molecular study of nine unrelated Chinese individuals and three families carrying B(A) alleles was conducted. Allele B(A)02 with a 700C>G mutation, allele B(A)04 with a single 640A>G substitution, and allele B(A)05 with a 641T>C mutation were detected in multigenerational families and unrelated blood donors. Neither the B(A)01 nor B(A)03 alleles with 703A>G substitutions were observed in this study. In addition, a polymerase chain reaction with a sequence-specific primer genotyping assay was developed for rapid identification of B(A)02, B(A)04, and B(A)05 alleles using genomic DNA samples. Immunohematology2007;23:69–74.
Improving care in care homes: A qualitative evaluation of the Croydon care home support team

Objectives: The Croydon care home support team (CHST) was developed in response to reports of patient abuse within long-term care. It presents a novel strategy for improving standards of care within care homes. A qualitative methodology was used to assess the perceived impact of the CHST. Method: In-depth interviews were conducted with 14 care home managers and 24 members of care home staff across 14 care homes. Grounded theory principles guided the collection and analysis of the data. Results: Reports of improved communication between staff, improved staff development and confidence, and improved quality of care point towards the effectiveness of the CHST model. The collaborative approach of the CHST was considered pivotal to its success and presented as an effective method of engaging care home managers and staff. The CHST adopted a systemic approach that placed an equal emphasis on the social, mental health and nursing needs of residents and aimed to address the whole culture of care within the individual homes. Conclusions: The data demonstrate the potential for specialist multi-disciplinary teams to raise standards of care across long-term care settings. Increased awareness of safeguarding issues, improved staff morale and communication and ongoing opportunities for discussion and problem solving promised to sustain improvements. Such services could be instrumental in meeting the government priority of preventing abuse among vulnerable adults.
Reconstructing the Clavariaceae using nuclear large subunit rDNA sequences and a new genus segregated from Clavaria

Fungi that produce clavarioid fruit bodies have evolved independently many times in the Basidiomycota. The evolutionary significance of this morphology is difficult to interpret because the phylogenetic positions of many clavarioid fungi are still unknown. In this study we examined the phylogenetic diversity of the Clavariaceae sensu lato among Homobasidiomycetidae by adding partial nuclear large subunit ribosomal DNA sequences from clavarioid and corticioid fungi to a large euagaric dataset and analyzing them both together and separately. Our results indicate that the clavarioid morphology has evolved at least five times in the euagarics while the inclusion of type species enabled us to evaluate the taxonomic consequences of this polyphyletic distribution. Although the sampling available at present is incomplete, a qualitative assessment of our phylogenetic hypotheses indicates that the clavarioid habit might not be as evolutionary labile as previously reported. We propose the new genus Alloclavaria to accommodate Clavaria purpurea, which is not related to Clavaria but is derived within the hymenochaetoid clade. The Physalacriaceae and Clavariaceae are redefined to reflect monophyletic groups, and the limits of Clavaria, Clavulinopsis and Ramariopsis should be reconsidered when additional data are available.
Mechanism of Free Radical Production in Exhaustive Exercise in Humans and Rats; Role of Xanthine Oxidase and Protection by Allopurinol

Exhaustive exercise generates free radicals. However, the source of this oxidative damage remains controversial. The aim of this paper was to study further the mechanism of exercise‐induced production of free radicals. Testing the hypothesis that xanthine oxidase contributes to the production of free radicals during exercise, we found not only that exercise caused an increase in blood xanthine oxidase activity in rats but also that inhibiting xanthine oxidase with allopurinol prevented exercise‐induced oxidation of glutathione in both rats and in humans. Furthermore, inhibiting xanthine oxidase prevented the increases in the plasma activity of cytosolic enzymes (lactate dehydrogenase, aspartate aminotransferase, and creatine kinase) seen after exhaustive exercise. Our results provide evidence that xanthine oxidase is responsible for the free radical production and tissue damage during exhaustive exercise. These findings also suggest that mitochondria play a minor role as a source of free radicals during exhaustive physical exercise.
NEURAL CLASSIFIER FOR ROBUST TRACKING Preparation of Camera-Ready Contributions to INSTICC Proceedings

This paper presents a method to fuse the information from motion segmentation with online adaptive neural classifier for robust object tracking. The motion segmentation with object classification identify new objects present in the video sequence. This information is used to initialize the online adaptive neural classifier which is learned to differentiate the object from its local background. The neural classifier can adapt to illumination variations and changes in appearance. Initialized objects are tracked in following frames using the fusion of their neural classifiers with the feedback from the motion segmentation. Fusion is used to avoid drifting problems due to similar appearance in the local background region. We demonstrate the approach in several experiments using benchmark video sequences with different level of complexity.
Assembly of optoelectronics for efficient chip-to-waveguide coupling

This paper presents two solutions to achieve efficient optical coupling between vertical cavity surface emitting lasers and planar optical waveguides, by minimizing the free space optical path length. One approach is based on embedding of optoelectronic chips in polymer layers, and the other approach on flip-chip assembly using micro-bumps. These micro-bumps are defined by laser-induced-forward-transfer (LIFT), a technique in which the bumping material can be transferred from a donor carrier to the waveguiding substrate. In both cases, out-of-plane bending of the light is accomplished by using a 45 degrees micro-mirror interface.
Physics of the Negative Resistance in the Avalanche  $I{-}V$  Curve of Field Stop IGBTs: Collector Design Rules for Improved Ruggedness

In this paper, we investigate the avalanche behavior of field-stop insulated gate bipolar transistors (IGBTs) by means of analytical and theoretical considerations, supported by ad hoc numerical simulations. A physical explanation of the presence of negative differential resistance branches in the avalanche I-V curve of the IGBT is presented and design criteria are derived to reduce and eventually eliminate this effect.
The case of synchronous occurrence of primary adenocarcinoma and squamous cell carcinoma in the same lobe of the lung

Synchronous occurrence of multiple lung cancers in the same lobe of the lung is very rare. Most of the tumors diagnosed in this way have the same histologic type. With imaging methods it is difficult to determine if the multiple lung lesions present hematogenous spread of lung cancer (or cancer from other origin) or these lesions present the second primary lung cancer. We report a rare and unusual case of synchronous occurrence of primary adenocarcinoma and squamous cell carcinoma in the same lobe of the lung. Our case demonstrates that in case of synchronous occurrence of multiple lung lesions each lesion should be sampled and histologic type of every lesion should be determined so the further treatment can be planned accordingly.
Modélisation des échanges radiatifs à l'échelle urbaine pour un urbanisme bioclimatique

L’objectif de cette these est de permettre une meilleure evaluation du bioclimatisme d’un projet d’amenagement urbain des les premieres etapes de la conception: de sa capacite a capter la lumiere naturelle a l’interieur des bâtiments, de l’efficacite energetique de ces derniers et du confort thermique des espaces exterieurs. Une premiere esquisse de la geometrie exterieure d’un projet est proposee des la phase concours, dans le plan masse. A ce stade, les bâtiments sont generalement assimiles a des prismes droits, avec eventuellement des toitures inclinees. Les details architecturaux tels que la forme et la position des fenetres, le cloisonnement interieur ou la composition des parois ne sont pas definis, et il est donc impossible de modeliser l’eclairage naturel a l’interieur des locaux ou encore le comportement thermique des enveloppes dans le plan masse. Nous montrons qu’il est toutefois possible d’identifier des cette etape les sources de rayonnement exterieures les plus favorables au bioclimatisme des bâtiments. Nous introduisons notamment les notions de luminances utiles et suffisantes, qui traduisent la part d’une annee pour laquelle une source de lumiere permet un eclairement adapte a l’interieur des locaux, et de temperatures radiantes, qui traduisent l’impact des sources de rayonnement de grandes et courtes longueurs d’ondes sur le rechauffement des surfaces et le confort thermique exterieur. Nous analysons leur distribution sur la voute celeste et mettons en evidence sa variabilite sous differents climats. L’objectif final de ce travail est de proposer un outil de modelisation a meme de guider les amenageurs dans leur recherche de formes urbaines bioclimatiques, des le plan masse.
Application of transmission electron microscopes to nanometre‐sized fabrication by means of electron beam‐induced deposition

Electron beam‐induced deposition was carried out using a scanning transmission electron microscope with a field emission gun to fabricate nanometre‐sized structures. A small amount of a metal–organic gas was introduced near the substrate in the microscope chamber, and focused electron beams were irradiated. Two‐ and three‐dimensional structures were fabricated by scanning the beam position. The minimum line width of the freestanding structures was 8 nm at a constant gas flux used. This line width of 8 nm is considered to be achieved by employing a high accelerating voltage, which leads to a small probe size, and the optimum scanning speed.
Limited arthrography in acute knee injuries

Fifty consecutive limited arthrograms in acute knee injuries are reported with operative findings in 27 knees. The arthrograms were completely accurate regarding cruciate and medial collat eral ligament integrity in 82% of all of the cases. Improvements in technique and recognition of superimposed anatomical struc tures led to fewer errors in interpretation as the series pro gressed. Limited arthrography is recommended as a useful office procedure in the early evaluation of knee ligament integ rity in acute painful knee injuries.
Exploiting tumor‐associated dendritic cell heterogeneity for novel cancer therapies

Dendritic cells (DCs) are specialized APCs present in all tissues, including tumors. They play a major role in orchestrating immune responses and were shown to occur in various functional states in tumors. In this respect, immunogenic tumor‐associated DCs (TADCs) are required to initiate and sustain T cell‐dependent anti‐cancer immunity, whereas regulatory TADCs harbor robust immunosuppressive potential and accelerate malignant growth. Importantly, the heterogeneity of the DC compartment in tumors has been dissected recently in murine and human cancers and was shown to consist of developmentally distinct subsets, including conventional DC (cDC)1, cDC2, and monocyte‐derived DCs (Mo‐DCs). TADCs constitute an essential target in efforts to generate therapeutic immunity against cancer, and the understanding of the complexity of the TADC heterogeneity might prove important for therapeutic interventions targeted at specific TADC subsets or their precursors. Hence, this review addresses the differential functional specializations of ontogenically distinct TADC subsets.
19.4 A 0.17-to-3.5mW 0.15-to-5GHz SoC PLL with 15dB built-in supply noise rejection and self-bandwidth control in 14nm CMOS

With recent advancements in SoC integration, modern SoC architectures can employ more than 20 PLLs [1]. To address SoC clocking needs with an ever reducing power budget, a deep sub-mW to low-mW PLL having a FoM between -226dB and -234dB from 0.8GHz to 5GHz is presented. The PLL features a modular implementation and therefore could be used as the local clock source or as part of a clock-generation hub. The hub provides reference clocks to subsystems from a single platform crystal oscillator through a combination of divisions and distributions.
Simulative study of random waypoint mobility model for mobile ad hoc networks

Connectivity of Mobile Ad-hoc Networks (MANET) is highly dependent on the mobility model it follows. From previous studies, it has been found that Random Waypoint Mobility Model (RWP) is widely used to simulate performance of the MANETs. This mobility model resembles the mobility of people using mobile phones in a given area. Connectivity is an important issue in a MANET following RWP model and hence researchers are trying out different ways to enhance connectivity. So, this model is considered and the variation of connectivity for different varying parameters is studied. The RWP model is simulated in MATLAB, followed by the study of the node connectivity by changing different parameters like number of user nodes, simulation time and simulation area. Further, it is shown that connectivity is enhanced in each case after introducing agent nodes. This study gives an estimate of number of nodes required for achieving desired connectivity of the network.
Integration of Large Photovoltaic and Wind System by Means of Smart Transformer

Large renewable energy systems, e.g., photovoltaic and wind turbine, are connected to grid, which is usually weak, with long lines. This degrades power system stability and reliability. Additionally, the magnetic characteristic of a conventional power transformer (CPT) implies a slow dynamic and leads to grid current harmonic too high when wind speed is low and irradiance is weak. This paper proposes a parallel connection of a solid-state transformer-based smart transformer and a CPT to not only reduce the power rating, but also to support the voltage amplitude and improve the grid current quality. The simulation and experimental results clearly verify the correctness and feasibility of the proposed strategy.
Activation of lecithin: cholesterol acyltransferase by HDL ApoA-I central helices

Abstract Lecithin:cholesterol acyltransferase (LCAT) is an enzyme that first hydrolyzes the sn-2 position of phospholipids, preferentially a diacylphosphocholine, and then transfers the fatty acid to cholesterol to yield a cholesteryl ester. HDL ApoA-I is the principal catalytic activator for LCAT. Activity of LCAT on nascent or lipid-poor HDL particles composed of phospholipid, cholesterol and ApoA-I allows the maturation of HDL particles into lipid-rich spherical particles that contain a core of cholesteryl ester surrounded by phospholipid and ApoA-I on the surface. This article reviews the recent progress in elucidating structural aspects of the interaction between LCAT and ApoA-I. In the last decade, there has been considerable progress in understanding the structure of ApoA-I and the central helices 5, 6, and 7 that are known to activate LCAT. However, much less information has been forthcoming describing the 3D structure and conformation of LCAT required to catalyze two separate reactions within a single monomeric peptide.
Optimization of Ship’s Route Scheduling Using Genetic Algorithm

Route scheduling is a quite complicated process because it involves some determinant factors. Several methods have been used to help resolve the NP-hard problems. This research uses genetic algorithm to assist in optimizing ship scheduling, that where there are several ports to be visited by some ships. The goal is to divide the ship to go to a specific port so that each port is only visited by one ship to minimize the total distance of all ships. The computational experiment produces optimal parameters such as the number of popsize is 30, the number of generations is 100, crossover rate value is 0.3 and mutation rate values is 0.7. The final results is an optimal ship route by minimizing the distance of each ship. Full Text: PDF DOI: http://dx.doi. org/10.11591/ijeecs.v2. i1.pp180-186
The Tom Core Complex

Translocation of nuclear-encoded preproteins across the outer membrane of mitochondria is mediated by the multicomponent transmembrane TOM complex. We have isolated the TOM core complex of Neurospora crassa by removing the receptors Tom70 and Tom20 from the isolated TOM holo complex by treatment with the detergent dodecyl maltoside. It consists of Tom40, Tom22, and the small Tom components, Tom6 and Tom7. This core complex was also purified directly from mitochondria after solubilization with dodecyl maltoside. The TOM core complex has the characteristics of the general insertion pore; it contains high-conductance channels and binds preprotein in a targeting sequence-dependent manner. It forms a double ring structure that, in contrast to the holo complex, lacks the third density seen in the latter particles. Three-dimensional reconstruction by electron tomography exhibits two open pores traversing the complex with a diameter of ∼2.1 nm and a height of ∼7 nm. Tom40 is the key structural element of the TOM core complex.
Signature verification using fractal transformation

Presents a fractal transformation based technique for signature verification. In this method, the fractal code of the reference signature locus is applied to a test signature locus and a sequence of fractal decoded test loci is obtained. The successive distances between the decoded test loci transforming towards the fractal attractor are compared to those obtained from the genuine reference signatures. The verification decision is derived from the rate of change of these distances. The experiment shows that the current implementation of the fractal transformation based verification algorithm is effective for random forgery detection.
Expanding Wikidata's Parenthood Information by 178%, or How To Mine Relation Cardinalities

While automated knowledge base construction so far has largely focused on fully qualified facts, e.g. 〈Obama, hasChild, Malia〉, the Web contains also extensive amounts of cardinality information, such as that someone has two children without giving their names. In this paper we argue that the extraction of such information could substantially increase the scope of knowledge bases. For the sample of the hasChild relation in Wikidata, we show that simple regular-expression based extraction from Wikipedia can increase the size of the relation by 178%. We also show how such cardinality information can be used to estimate the recall of knowledge bases.