Miracle-dz/UniFAR-FTUs huggingface dataset:
Pre‐operative factors predicting good outcome in terms of health‐related quality of life after ACL reconstruction

The life situation of many patients changes after an anterior cruciate ligament (ACL) rupture and subsequent reconstruction, and this may affect their health‐related quality of life in many ways. It is well known that the overall clinical results after ACL reconstruction are considered good, but pre‐operative predictive factors for a good post‐operative clinical outcome after ACL reconstruction have not been studied in as much detail. The purpose of this study was to identify pre‐operative factors that predict a good post‐operative outcome as measured by the Short Form 36 (SF‐36) and Knee Osteoarthritis Outcome Score (KOOS) 3–6 years after ACL reconstruction. Seventy‐three patients scheduled for ACL reconstruction were clinically examined pre‐operatively. The SF‐36 and KOOS questionnaires were sent by mail to these patients 3–6 years after reconstruction. Predictive factors for health‐related quality of life were investigated using a stepwise regression analysis. In conclusion, pre‐operative factors, such as pivot shift, knee function, and range of motion, may predict a good post‐operative outcome and explain up to 25% in terms of health‐related quality of life after ACL reconstruction. Furthermore, it appears that the patients’ pre‐injury and pre‐operative Tegner activity levels are important predictors of post‐operative health‐related quality of life.
tRNA-Related Sequences Trigger Systemic mRNA Transport in Plants[OPEN]

Specific tRNA-derived motifs present in an endogenous dicistronic transcript mediate mRNA transport along the phloem. In plants, protein-coding mRNAs can move via the phloem vasculature to distant tissues, where they may act as non-cell-autonomous signals. Emerging work has identified many phloem-mobile mRNAs, but little is known regarding RNA motifs triggering mobility, the extent of mRNA transport, and the potential of transported mRNAs to be translated into functional proteins after transport. To address these aspects, we produced reporter transcripts harboring tRNA-like structures (TLSs) that were found to be enriched in the phloem stream and in mRNAs moving over chimeric graft junctions. Phenotypic and enzymatic assays on grafted plants indicated that mRNAs harboring a distinctive TLS can move from transgenic roots into wild-type leaves and from transgenic leaves into wild-type flowers or roots; these mRNAs can also be translated into proteins after transport. In addition, we provide evidence that dicistronic mRNA:tRNA transcripts are frequently produced in Arabidopsis thaliana and are enriched in the population of graft-mobile mRNAs. Our results suggest that tRNA-derived sequences with predicted stem-bulge-stem-loop structures are sufficient to mediate mRNA transport and seem to be necessary for the mobility of a large number of endogenous transcripts that can move through graft junctions.
cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases

A human epithelial (HeLa) cDNA library was screened with degenerate oligonucleotides designed to hybridize to highly conserved regions of protein-tyrosine kinases. One cDNA from this screen was shown to contain a putative protein-tyrosine kinase catalytic domain and subsequently used to isolate another cDNA from a human keratinocyte library that encompasses the entire coding region of a 976-amino-acid polypeptide. The predicted protein has an external domain of 534 amino acids with a presumptive N-terminal signal peptide, a transmembrane domain, and a cytoplasmic domain of 418 amino acids that includes a canonical protein-tyrosine kinase catalytic domain. Molecular phylogeny indicates that this protein kinase is closely related to eph and elk and that this receptor family is more closely related to the non-receptor protein-tyrosine kinase families than to other receptor protein-tyrosine kinases. Antibodies raised against a TrpE fusion protein immunoprecipitated a 130-kDa protein that became phosphorylated on tyrosine in immune complex kinase assays, indicating that this protein is a bona fide protein-tyrosine kinase. Analysis of RNA from 13 adult rat organs showed that the eck gene is expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary. Several cell lines of epithelial origin were found to express the eck protein kinase at the protein and RNA levels. Immunohistochemical analysis of several rat organs also showed staining in epithelial cells. These observations prompted us to name this protein kinase eck, for epithelial cell kinase.
A low-voltage ride-through control strategy for three-phase grid-connected PV systems

This paper proposes a novel control strategy for the two-stage three-phase Photovoltaic (PV) systems to improve the operation under unbalanced grid faults. In case that a three-phase power converter operates under unbalanced grid voltage faults, power related issues, including injected currents, active and reactive power can be negatively affected. In grid-connected systems, the unbalanced faults may lead to Double Grid Frequency (DGF) oscillations of active and/or reactive power or injection of non-sinusoidal three-phase currents. The DGF oscillations of the injected active power can cause similar variations in the dc-link voltage of conventional two-stage PV inverters. In such systems with an electrolytic capacitor in the dc-link, the oscillations of the dc-link voltage with DGF can deteriorate the capacitor lifetime, and thus the entire system. The proposed Low-Voltage Ride-Through (LVRT) control strategy benefits from a reference current generation method, which can eliminate the oscillations at the dc-link and in the active power during unbalanced voltage dips. In addition, a reliable current limiting method is introduced, which can efficiently prevent overcurrent failure under grid faults. Performance of the proposed LVRT strategy is verified on a 2-kW system by simulations under unbalanced voltage dips.
A Separation Principle for Underactuated Lossless Lagrangian Systems

We study under-actuated Lagrangian systems without dissipative forces, augmented by a chain of integrators. For such systems, we solve the open problem of global tracking control via position measurements only; strictly speaking, we establish uniform global asymptotic stability for the closed loop system. As a corollary, we obtain an original statement for flexible-joint robots, which closes a long-standing open problem of output feedback nonlinear control.
Recommender Response to Diversity and Popularity Bias in User Profiles

– – Recommender Response to Diversity and Popularity Bias in User Profiles Sushma Channamsetty Michael D. Ekstrand Department of Computer Science Texas State University San Marcos, TX USA sushmachannamsetty@gmail.com People and Information Research Team Department of Computer Science, Boise State University, Boise, ID USA michaelekstrand@boisestate.edu Proceedings of the Thirtieth International Florida Artificial Intelligence Research Society Conference
Epidemiology of Rheumatoid Arthritis (RA) in rural and urban areas of Poland - 2008-2012.

OBJECTIVE The aim of this study is to investigate the morbidity rate due to Rheumatoid Arthritis (RA) in the Polish population during 2008-2012, calculated per 1,000 inhabitants, and taking into account the differences between provincess, area of residence (urban or rural) and gender. MATERIALS AND METHOD From the NFZ IT systems, PESEL number information was obtained for all 17 types of services contracted in 2008-2012, for patients whose main diagnosis in the report was the ICD-10 disease code: M05.X - seropositive rheumatoid arthritis, or M06.X - other rheumatoid arthritis. The number of patients, gender and age were calculated based on the PESEL number provided in the statistical reports of the patient with the analysed ICD-10 diagnosis. Urban and rural cases were compared using commune zip codes. The basis for classifying the patient as a member of an urban or rural population was the Zip Code of the declared place of residence. Urban and rural areas are classified based on administrative criteria provided by the Central Statistical Office: the National Official Register of Territorial Division of the Country (TERYT). RESULTS During the studied period the number of RA patients increased from 173,844-230,892. In urban areas, the most patients were recorded in the Śląskie Province, the least in Lubuskie Province. Patients from rural areas were approx. 1/3(rd) of the total population of patients in Poland. In rural areas, the most patients were recorded in the Mazowieckie Province, the least in Lubuskie Province. The morbidity rate in cities was 5.08 in 2008 and increased to 8.14 in 2012 in rural areas, respectively, it was 3.74 and increased to 3.98. Regardless of the place of residence the women fell ill 3.5 times more frequently. The lowest morbidity rate, both in rural and urban areas, was recorded in the Lubuskie Province, the largest in Świętokrzyskie Province. The the most probable explanation of the highest morbidity rate in the latter province is a worse access to a rheumatologist: in this province there is the lowest number of inhabitants per one employed rheumatologist. CONCLUSION In Poland, the number of RA sufferers is increasing, which is probably a result of increasing life expectancy. In Poland, also exists a differences in morbidity between urban and rural inhabitants. Differences may also derive from undiagnosed cases of the disease.
Notch inhibition in Kaposi's sarcoma tumor cells leads to mitotic catastrophe through nuclear factor-κB signaling

Kaposi's sarcoma (KS) is the most common neoplasm in untreated AIDS patients and accounts for significant morbidity and mortality worldwide. We have recently reported that Notch signaling (which plays an important role in cell proliferation, apoptosis, and oncogenesis) is constitutively activated in KS tumor cells. Blockade of this activity using γ-secretase inhibitors resulted in apoptosis of SLK cells, a KS tumor cell line; however, this apoptosis was preceded by a prolonged G2-M cell cycle arrest. This result led us to hypothesize that the cells were undergoing mitotic catastrophe, an abnormal mitosis that leads to eventual cell death. Here, we show that Notch inhibition in KS tumor cells using γ-secretase inhibitors or Notch-1 small interfering RNA resulted in G2-M cell cycle arrest and mitotic catastrophe characterized by the presence of micronucleated cells and an increased mitotic index. Interestingly, Notch inhibition led to a sustained increase in nuclear cyclin B1, a novel observation suggesting that Notch signaling can modulate expression of this critical cell cycle protein. Further analysis showed the induction of cyclin B1 was due, at least in part, to increased nuclear factor-κB (NF-κB) activity, which was also required for the G2-M growth arrest after Notch inhibition. Taken together, these studies suggest that Notch inhibition can initiate aberrant mitosis by inducing NF-κB activity that inappropriately increases cyclin B1 resulting in cell death via mitotic catastrophe. [Mol Cancer Ther 2007;6(7):1983–92]
Complications of Endovascular Treatment for Acute Stroke in the SWIFT Trial with Solitaire and Merci Devices

The safety of 2 devices to treat acute ischemic stroke was evaluated in 144 patients with special attention paid to complications such as cerebral hemorrhage, SAH, air emboli, vessel dissections, major groin complications, and emboli in new territories. Fewer endovascular complications occurred after treatment with the Solitaire device when compared with the Merci, particularly with respect to symptomatic cerebral hemorrhage. BACKGROUND AND PURPOSE: Treatment of patients with ischemic stroke after endovascular treatment requires in-depth knowledge of complications. The goal of this study was to make endovascular treatment for acute ischemic stroke safer through an in-depth review of the major periprocedural complications observed in the Solitaire FR With Intention for Thrombectomy (SWIFT) trial. MATERIALS AND METHODS: The SWIFT data base was searched for major peri-procedural complications defined as symptomatic intracranial hemorrhage within 36 hours, SAH, air emboli, vessel dissection, major groin complications, and emboli to new vascular territories. RESULTS: Major peri-procedural complications occurred in 18 of 144 patients (12.5%) as follows: symptomatic intracranial hemorrhage, 4.9%; air emboli, 1.4%; vessel dissection, 4.2%; major groin complications, 2.8%; and emboli to new vascular territories, 0.7%. Rates of symptomatic intracranial bleeding by subtype were PH1, 0.7%; PH2, 0.7% (PH1 indicates hematoma within ischemic field with some mild space-occupying effect but involving ≤30% of the infarcted area; PH2, hematoma within ischemic field with space-occupying effect involving >30% of the infarcted area); intracranial hemorrhage remote from ischemic zone, 0%; intraventricular hemorrhage, 0.7%; and SAH, 3.5%. We did not observe any statistically significant associations of peri-procedural complications with age; type of treatment center; duration of stroke symptoms; NIHSS score, IV thrombolytics, atrial fibrillation, site of vessel occlusion; rescue therapy administered after endovascular treatment; or device. Comparing the Merci with the Solitaire FR retrieval device, we observed symptomatic cerebral hemorrhage (10.9% versus 1.1%; P = .013); symptomatic SAH (7.3% versus 1.1%; P = .07), air emboli (1.8% versus 1.1%; P = 1.0), emboli to new vascular territories (1.8% versus 0%; P = .38), vessel dissection (1.8% versus 4.5%; P = .65), and major groin complications (3.6% versus 7.9%; P = .48). Angiographic vasospasm was common but without clinical sequelae. CONCLUSIONS: Understanding of procedural complications is important for treatment of patients with stroke after endovascular treatment. We observed fewer endovascular complications with the Solitaire FR device treatment compared with Merci device treatment, particularly symptomatic cerebral hemorrhage.
Motivational determinates of creative tourism: a case study of Albergue art space in Macau

ABSTRACT Creative tourism is a new genre of tourism eliciting active tourist participation in learning and experiences. This study explores tourists’ motivations for participating in creative activities, as well as their perceptions of authenticity following a visit to the Albergue Art Space located in Macau, SAR, China. Empirical results indicate that vernacular heritage, service quality and participatory experience are key determinates for developing creative tourism. Sightseeing, social contact, self-improvement and escape emerge as primary motivations for participating in creative activities. In addition, tourist perceptions of authenticity in the context of creative tourism encompass objective and existential components. This study proposes to incorporate aspects of participatory experience into creative tourism products.
Role of TrfA and DnaA Proteins in Origin Opening during Initiation of DNA Replication of the Broad Host Range Plasmid RK2*

The Escherichia coli protein DnaA and the plasmid RK2-encoded TrfA protein are required for initiation of replication of the broad host range plasmid RK2. The TrfA protein has been shown to bind to five 17-base pair repeat sequences, referred to as iterons, at the minimal replication origin (oriV). Using DNase I footprinting and a gel mobility shift assay, purified DnaA protein was found to bind to four DnaA consensus binding sequences immediately upstream of the five iterons at the RK2 origin of replication. Binding of the TrfA protein to the iterons results in localized strand opening within the A+T-rich region of the replication origin as determined by reactivity of the top and bottom strands to potassium permanganate (KMnO4). The presence of either theE. coli DnaA or HU protein is required for the TrfA-mediated strand opening. Although the DnaA protein itself did not produce an RK2 open complex, it did enhance and/or stabilize the TrfA-induced strand opening.
Clinical applications of pulse transit time in paediatric critical care

A simple and non-invasive technique, termed pulse transit time (PTT), has shown its potential in long-term investigations such as respiratory sleep studies and cardiovascular studies. Based on these findings, the PTT technique shows relevance for continuous haemodynamic monitoring in critical care. The objective of this review is to understand the potential, applications and limitations of PTT in this clinical setting. Present non-invasive haemodynamic monitoring methods such as automated oscillometric blood pressure (BP) and auscultatory techniques have their known limitations. They tend to underestimate systolic BP while overestimating diastolic BP. Due to the periodic increase in cuff pressure cycles during data acquisition, these techniques may cause much discomfort in elderly geriatric patients, or lessen the cooperation of younger paediatric patients. Thus, there can be adverse effects on therapeutic decisions and possibly clinical outcomes. Documented evidences have indicated that changes observed in PTT are inversely correlated to the corresponding BP changes. In critical care, a simple and accommodating technique like PTT may be useful in providing better comfort for patients during extended monitoring. Being a semi-quantitative measure, blanket recommendations for its utility can then become possible. The basic instrumentations needed are often part of standard critical care monitoring system. Furthermore, PTT also has the potential to monitor the often tachypnoeic respiratory dependent BP changes seen in small infants during critical care.
Interactions between Adaptor Protein-1 of the Clathrin Coat and Microtubules via Type 1a Microtubule-associated Proteins*

The classical view suggests that adaptor proteins of the clathrin coat mediate the sorting of cargo protein passengers into clathrin-coated pits and the recruitment of clathrin into budding areas in the donor membrane. In the present study, we provide biochemical and morphological evidence that the adaptor protein 1 (AP-1) adaptor of the trans-Golgi network clathrin interacts with microtubules. AP-1 in cytosolic extracts interacted within vitro assembled microtubules, and these interactions were inhibited by ATP depletion of the extracts or in the presence of 5′-adenylylimidodiphosphate. An overexpressed γ-subunit of the AP-1 complex associated with microtubules, suggesting that this subunit may mediate the interaction of AP-1 with the cytoskeleton. Purified AP-1 did not interact with purified microtubules, but interaction occurred when an isolated microtubule-associated protein fraction was added to the reaction mix. The γ-adaptin subunit of AP-1 specifically co-immunoprecipitated with a microtubule-associated protein of type 1a from rat brain cytosol. This suggests that type 1a microtubule-associated protein may mediate the association of AP-1 with microtubules in the cytoplasm. The microtubule binding activity of AP-1 was markedly inhibited in cytosol of mitotic cells. By means of its interaction with microtubule-associated proteins, we propose novel roles for AP-1 adaptors in modulating the dynamics of the cytoskeleton, the stability and shape of coated organelles, and the loading of nascent AP-1-coated vesicles onto appropriate microtubular tracks.
Utility Outcome Scores for Unilateral Facial Paralysis

BackgroundFacial paralysis is a debilitating condition. Dynamic and static facial reanimation remains a challenge for plastic surgeons and requires important resources. Our objective was to quantify the health state utility assessment (ie, utility score outcomes) of living with unilateral facial paralysis. MethodsUtility assessments using visual analog scale, time trade-off, and standard gamble were used to obtain utility outcome scores for unilateral facial paralysis from a prospective sample of the general population and medical students. ResultsA total number of 123 individuals prospectively participated in the study. All measures (visual analog scale, time trade-off, and standard gamble) for unilateral facial paralysis [0.56 ± 0.18, 0.78 ± 0.21, and 0.79 ± 0.21 respectively] were significantly different (P < 0.0001) from the corresponding outcome scores for monocular blindness [0.61 ± 0.21, 0.83 ± 0.21, and 0.85 ± 0.18, respectively] and binocular blindness [0.33 ± 0.18, 0.65 ± 0.28, and 0.65 ± 0.29, respectively]. Linear regression analysis using age, race, income, and education as predictors of each of the utility scores for facial paralysis showed no statistical significance. ConclusionsIn samples of the general population and medical students, all utility score outcome measures for facial paralysis were lower than those for monocular blindness. Our sample population, if faced with unilateral facial paralysis, would theoretically undergo facial reanimation procedures with a willingness to sacrifice 8 years of life and be willing to undergo a procedure with a 21% chance of mortality to attain perfect health, respectively.
Walking the Walk: Changing Familial Forms, Government Policy and Everyday Social Work Practice in England

Although contemporary sociological thought reports a diversification of family forms in society, ‘the family’ continues to influence national and international political agendas. Social workers, as ‘street level bureaucrats’, are social agents that both work with citizens and implement policies made by senior officials. Despite this, the extent to which conceptual and policy developments in family diversity manifest in family-based social work practice remains under explored. This article brings together the findings of two comparative studies, and explores the transfer of conceptual understandings of family, and policy, in England, through two examples: gendered caring expectations and culturally located familial norms. Significantly, we show that, when prompted, social workers recognise family complexity and diversity, but myriad constraints complicate the application of these understandings, and related policies. Bringing together literature from sociology, social policy and social work, this article, thereby, offers a unique lens and highlights a lag between conceptual developments, policy and implementation.