Miracle-dz/UniFAR-FTUs huggingface dataset:
Extending the Cortical Grasping Network: Pre-supplementary Motor Neuron Activity During Vision and Grasping of Objects

Grasping relies on a network of parieto-frontal areas lying on the dorsolateral and dorsomedial parts of the hemispheres. However, the initiation and sequencing of voluntary actions also requires the contribution of mesial premotor regions, particularly the pre-supplementary motor area F6. We recorded 233 F6 neurons from 2 monkeys with chronic linear multishank neural probes during reaching–grasping visuomotor tasks. We showed that F6 neurons play a role in the control of forelimb movements and some of them (26%) exhibit visual and/or motor specificity for the target object. Interestingly, area F6 neurons form 2 functionally distinct populations, showing either visually-triggered or movement-related bursts of activity, in contrast to the sustained visual-to-motor activity displayed by ventral premotor area F5 neurons recorded in the same animals and with the same task during previous studies. These findings suggest that F6 plays a role in object grasping and extend existing models of the cortical grasping network.
Root coverage procedures improve patient aesthetics. A systematic review and Bayesian network meta-analysis.

BACKGROUND The aim of this study was to perform a systematic review (SR) of randomized controlled trials (RCTs) to explore if periodontal plastic surgery procedures for the treatment of single and multiple gingival recessions (Rec) may improve aesthetics at patient and professional levels. MATERIAL AND METHODS In order to combine evidence from direct and indirect comparisons by different trials a Bayesian network meta-analysis (BNM) was planned. A literature search on PubMed, Cochrane libraries, EMBASE, and hand-searched journals until January 2016 was conducted to identify RCTs presenting aesthetic outcomes after root coverage using standardized evaluations at patient and professional level. RESULTS A total of 16 RCTs were selected in the SR; three RTCs presenting professional aesthetic evaluation with Root coverage Aesthetic Score (RES) and three showing final self-perception using the Visual Analogue Scale (VAS Est) could be included in a BNM model. Coronally Advanced Flap plus Connective Tissue Graft (CAF + CTG) and CAF + Acellular Dermal Matrix (ADM) and Autologous Fibroblasts (AF) were associated with the best RES outcomes (best probability = 24% and 64%, respectively), while CAF + CTG and CAF + CTG + Enamel matrix Derivatives (EMD) obtained highest values of VAS Est score (best probability = 44% and 26%, respectively). CONCLUSIONS Periodontal Plastic Surgery (PPS) techniques applying grafts underneath CAF with or without the adding of EMD are associated with improved aesthetics assessed by final patient perception and RES as professional evaluation system.
Effects of body mass index and education on verbal and nonverbal memory

ABSTRACT We previously reported that higher education protects against executive dysfunction related to higher body mass index (BMI) in younger, but not older, adults. We now extend the previous analyses to verbal and nonverbal memory. Fifty-nine healthy, dementia-free community-dwelling adults ranging in age from 18 to 81 years completed the Hopkins Verbal Learning Test – Revised (HVLT-R) and the Brief Visuospatial Memory Test – Revised (BVMT-R). Self-reported years of education served as a proxy for cognitive reserve. We found that more highly educated individuals maintained their BVMT-R immediate recall performance across the range of BMI, but in less educated individuals, higher BMI was associated with worse performance. Our findings suggest that education may play a protective role against BMI-related nonverbal learning deficits, similar to previous reports for verbal memory and executive functioning. Results highlight the importance of considering educational background when determining the risk for BMI-related cognitive impairment in clinical settings.
Aromatic dipeptide Trp–Ala can be transported by Arabidopsis peptide transporters AtPTR1 and AtPTR5

ABSTRACT Dipeptides with an aromatic residue at the N–terminal position induced lower inward currents or blocked leak currents in Xenopus oocytes expressing the proton-coupled peptide transporter AtPTR1 or AtPTR5 of Arabidopsis thaliana compared with dipeptides with an aromatic residue at the C–terminal position. Here, AtPTR1 and AtPTR5 were expressed in a yeast mutant of peptide transporter (ptr2) with tryptophan auxotrophy. Growth assays showed that Trp–Ala could be transported by both AtPTR1 and AtPTR5 as efficiently as Ala–Trp. Our data suggested that the previous finding in Xenopus oocytes might be an artifact of heterologous expression, and that AtPTR1 and AtPTR5 expressed in yeast could transport dipeptides with an aromatic residue at the N–terminal position.
Species Differences in the Organization of the Ventral Cochlear Nucleus

The mammalian cochlear nuclei (CN) consist of two major subdivisions, the dorsal (DCN) and ventral (VCN) nuclei. We previously reported differences in the structural and neurochemical organization of the human DCN from that in several other species. Here we extend this analysis to the VCN, considering both the organization of subdivisions and the types and distributions of neurons. Classically, the VCN in mammals is composed of two subdivisions, the anteroventral (VCA) and posteroventral cochlear nuclei (VCP). Anatomical and electrophysiological data in several species have defined distinct neuronal types with different distributions in the VCA and VCP. We asked if VCN subdivisions and anatomically defined neuronal types might be distinguished by patterns of protein expression in humans. We also asked if the neurochemical characteristics of the VCN are the same in humans as in other mammalian species, analyzing data from chimpanzees, macaque monkeys, cats, rats and chinchillas. We examined Nissl‐ and immunostained sections, using antibodies that had labeled neurons in other brainstem nuclei in humans. Nissl‐stained sections supported the presence of both VCP and VCA in humans and chimpanzees. However, patterns of protein expression did not differentiate classes of neurons in humans; neurons of different soma shapes and dendritic configurations all expressed the same proteins. The patterns of immunostaining in macaque monkey, cat, rat, and chinchilla were different from those in humans and chimpanzees and from each other. The results may correlate with species differences in auditory function and plasticity. Anat Rec, 301:862–886, 2018. © 2017 Wiley Periodicals, Inc.
Water transport through the intestinal epithelial barrier under different osmotic conditions is dependent on LI-cadherin trans-interaction

ABSTRACT In the intestine water has to be reabsorbed from the chymus across the intestinal epithelium. The osmolarity within the lumen is subjected to high variations meaning that water transport often has to take place against osmotic gradients. It has been hypothesized that LI-cadherin is important in this process by keeping the intercellular cleft narrow facilitating the buildup of an osmotic gradient allowing water reabsorption. LI-cadherin is exceptional among the cadherin superfamily with respect to its localization along the lateral plasma membrane of epithelial cells being excluded from adherens junction. Furthermore it has 7 but not 5 extracellular cadherin repeats (EC1-EC7) and a small cytosolic domain. In this study we identified the peptide VAALD as an inhibitor of LI-cadherin trans-interaction by modeling the structure of LI-cadherin and comparison with the known adhesive interfaces of E-cadherin. This inhibitory peptide was used to measure LI-cadherin dependency of water transport through a monolayer of epithelial CACO2 cells under various osmotic conditions. If LI-cadherin trans-interaction was inhibited by use of the peptide, water transport from the luminal to the basolateral side was impaired and even reversed in the case of hypertonic conditions whereas no effect could be observed at isotonic conditions. These data are in line with a recently published model predicting LI-cadherin to keep the width of the lateral intercellular cleft small. In this narrow cleft a high osmolarity can be achieved due to ion pumps yielding a standing osmotic gradient allowing water absorption from the gut even if the faeces is highly hypertonic.
Nebivolol alleviates aortic remodeling through eNOS upregulation and inhibition of oxidative stress in l-NAME-induced hypertensive rats

ABSTRACT Purpose: To investigate the effect and mechanism of nebivolol on aortic remodeling in N-nitro-l-arginine methyl ester (l-NAME)-induced hypertension. Methods: Male Sprague–Dawley rats were treated with equal volumes of drinking water or l-NAME (60 mg/kg/day), alone or in combination with nebivolol (8 mg/kg/day) or atenolol (80 mg/kg/day) by gavage for 8 weeks. Systolic blood pressure (SBP), aortic morphometry, plasma nitric oxide (NO) levels, nitric oxide synthase (NOS) activity, and relaxation of aorta to acetylcholine were determined. Protein expression of endothelial NOS (eNOS), Akt, and NADPH oxidase (Nox) was evaluated. Results: l-NAME-treated rats showed an elevated SBP associated with aortic remodeling. l-NAME-treated rats showed reduced plasma NO levels and NOS activity and increased reactive oxygen species (ROS). Protein expression of eNOS, eNOS phosphorylated at Ser1177 (p-eNOS), Akt, and Akt phosphorylated at Ser473 (p-Akt) decreased, whereas that of Nox2, Nox4, and p22phox increased in the aortas from l-NAME-treated rats. Nebivolol treatment reduced SBP and ameliorated aortic remodeling. The effects of nebivolol were accompanied by increasing NO levels, NOS activity, and expression of eNOS, p-eNOS, Akt, and p-Akt, as well as reduction of ROS generation and Nox2, Nox4, and p22phox expression. These effects of nebivolol were not reproduced by atenolol. Conclusion: Our data indicate a protective role of nebivolol on the high blood pressure and vascular remodeling induced by l-NAME. The beneficial vascular effect of nebivolol is mediated by the upregulation of eNOS and inhibition of oxidative stress.
PTEN loss is associated with prostate cancer recurrence and alterations in tumor DNA methylation profiles

Background Prostate cancer (PCa) with loss of the tumor suppressor gene PTEN has an unfavorable prognosis. DNA methylation profiles associated with PTEN loss may provide further insights into the mechanisms underlying these more aggressive, clinically relevant tumors. Methods The cohort included patients with clinically localized PCa. Samples taken from the primary tumor were used to determine PTEN genomic deletions using FISH, and to analyze epigenome-wide DNA methylation profiles. Patients were followed for PCa recurrence on average for 8 years after diagnosis. Results The study included 471 patients with data on PTEN loss, and the frequency of hemi- and homozygous PTEN loss was 10.0% and 4.5%, respectively. Loss of PTEN was associated with a significantly higher risk of recurrence (any vs. no PTEN loss; HR = 1.74; 95% CI: 1.03–2.93). Hazard ratios for hemi- and homozygous loss were 1.39 (95% CI: 0.73–2.64) and 2.84 (95% CI: 1.30–6.19), respectively. Epigenome-wide methylation profiling identified 4,208 differentially methylated CpGs (FDR Q-value < 0.01) in tumors with any versus no PTEN loss. There were no genome-wide significant differentially methylated CpGs in homo- versus hemizygous deleted tumors. Tumor methylation data were used to build a methylation signature of PTEN loss in our cohort, which was confirmed in TCGA, and included CpGs in ATP11A, GDNF, JAK1, JAM3, and VAPA. Conclusion Loss of PTEN was positively associated with PCa recurrence. Prostate tumors with PTEN loss harbor a distinct methylation signature, and these aberrantly methylated CpG sites may mediate tumor progression when PTEN is deleted.
Pharmacological blockage of the CXCR4-CXCL12 axis in endometriosis leads to contrasting effects in proliferation, migration, and invasion†

Abstract High levels of inflammatory factors including chemokines have been reported in peritoneal fluid and blood of women with endometriosis. CXCL12 mediates its action by interaction with its specific receptor, CXCR4, reported to be elevated in human endometriosis lesions and in the rat model of endometriosis. Activation of the CXCR4-CXCL12 axis increases cell proliferation, migration, and invasion of cancer cells. To obtain insights into the CXCR4 expression profile in lesions and endometrium, as well as functionality of the CXCR4-CXCL12 axis in endometriosis, we analyzed the expression of CXCR4 in tissues on a human tissue array and studied CXCL12- mediated activation of proliferation, invasion, and migration in vitro. We observed differences in levels of nuclear CXCR4 expression among lesion types, being higher in ovarian lesions. Endometriotic cell lines (12Z) showed higher levels of CXCR4, proliferative and migratory potential, and AKT phosphorylation/kinase activity compared to untreated control cells (endometrial epithelial cells). CXCL12 and endometriotic stromal cell-enriched media increased proliferation of non-endometriotic epithelial cells. CXCL12 caused a significant increase in 12Z cell invasion but had no effect on migration; AMD3100, a CXCR4-specific inhibitor, significantly increased invasion of 12Z cells but decreased their migration. However, treatment with CXCL12 plus AMD3100 significantly decreased invasion and migration of 12Z cells. In conclusion, the CXCR4-CXCL12 axis is functional in endometriosis cells, but the expression of CXCR4 varies among lesions. CXCL12 promoted proliferation, migration, and invasion of endometriotic cells, while inducing AKT phosphorylation and activity, but pharmacologically blocking this axis in the absence of the ligand induced their invasiveness. Summary Sentence CXCL12 treatment of endometriotic cells promoted their proliferation, migration, and invasion, while inducing AKT phosphorylation and activity, but pharmacologically blocking this axis in the absence of the ligand increased their invasion capacity.
Optimal Reliability in Energy Harvesting Industrial Wireless Sensor Networks

For industrial wireless sensor networks, it is essential to reliably sense and deliver the environmental data on time to avoid system malfunction. While energy harvesting is a promising technique to extend the lifetime of sensor nodes, it also brings new challenges for system reliability due to the stochastic nature of the harvested energy. In this paper, we investigate the optimal energy management policy to minimize the weighted packet loss rate under the delay constraint, where the packet loss rate considers the lost packets, both during the sensing and delivering processes. We show that the above-mentioned energy management problem can be modeled as an infinite horizon average reward constraint Markov decision problem. In order to address the well-known curse of dimensionality problem and facilitate distributed implementation, we use the linear value approximation technique. Moreover, we apply stochastic online learning with a post-decision state to deal with the lack of the knowledge of the underlying stochastic processes. A distributed energy allocation algorithm with a water-filling structure and a scheduling algorithm by an auction mechanism are obtained. Experimental results show that the proposed algorithm achieves nearly the same performance as the optimal offline value iteration algorithm while requiring much less computation complexity and signaling overhead, and outperforms various existing baseline algorithms.
A Low-Cost IoT Application for the Urban Traffic of Vehicles, Based on Wireless Sensors Using GSM Technology

Congestion of vehicular traffic in the cities with the highest population density results into a high amount of accidents and also to imminent road violence above average. Facing this problem, our study intends to implement a low-cost Internet of Thing proposal, in order to monitor and analyze traffic circulation and provide solutions to reduce the above mentioned negative effects. To fulfill such proposal, a system of low-cost wireless monitoring has been designed and developed, which is supported on a distributed multilayer model. This system interacts with a device consisting of two electronic Arduino platforms, which themselves interact in master-slave mode having a distance sensor based on laser for detection of vehicles. The latter has the ability to connect to the Internet transmitting in real-time data taken by HTTP requests over the cellular network. On the data collected an engine for data mining is mounted in order to receive information about the traffic on highways and subsequently proposing potential solutions. The proof of concept has been applied in the city of Quito. There, on over their two central lanes of the Simon Bolivar Avenue such kind of solutions have been applied with positive results, both in the operation of the software and hardware that compose the prototype, as well as in reference measurements of traffic. Based on our findings, the system manages to suggest extrapolations, which enables to reduce traffic congestion, fuel waste, and also air pollution.
Smart guiding glasses for visually impaired people in indoor environment

To overcome the travelling difficulty for the visually impaired group, this paper presents a novel ETA (Electronic Travel Aids)-smart guiding device in the shape of a pair of eyeglasses for giving these people guidance efficiently and safely. Different from existing works, a novel multi-sensor fusion based obstacle avoiding algorithm is proposed, which utilizes both the depth sensor and ultrasonic sensor to solve the problems of detecting small obstacles, and transparent obstacles, e.g. the French door. For totally blind people, three kinds of auditory cues were developed to inform the direction where they can go ahead. Whereas for weak sighted people, visual enhancement which leverages the AR (Augment Reality) technique and integrates the traversable direction is adopted. The prototype consisting of a pair of display glasses and several low-cost sensors is developed, and its efficiency and accuracy were tested by a number of users. The experimental results show that the smart guiding glasses can effectively improve the user’s travelling experience in complicated indoor environment. Thus it serves as a consumer device for helping the visually impaired people to travel safely.
A scan partitioning algorithm for reducing capture power of delay-fault LBIST

It is well-known that high power consumption in test mode can cause problems such as overheating and IR-drop which have negative effect on circuit reliability and yield. The problem is particularly hard in the case of at-speed delay-fault testing where it cannot be mitigated by lowering the clock frequency. The difficulty increases even further if pseudo-random rather than ATPG patterns are used for testing. ATPG patterns can be chosen selectively, as well as re-ordered and specified in a power-friendly manner. Pseudo-random test patterns are much harder to control. In this paper, we present a scan partitioning algorithm for reducing capture power targeting delay-fault LBIST. The algorithm uses a novel weighted S-graph model in which the weights are determined by signal probability analysis. Our experimental results show that, on average, the presented method reduces average capture power by 50% and peak capture power by 39% with less than 2% loss in the transition fault coverage.
Multiscale mixing patterns in networks

Significance A central theme of network science is the heterogeneity present in real-life systems, for instance through the absence of a characteristic degree for the nodes. Despite their small-worldness, networks may present other types of heterogeneous patterns, with different parts of the network exhibiting different behaviors. Here we focus on assortativity, a network analogue of correlation used to describe how the presence and absence of edges covaries with the properties of nodes. We design a method to characterize the heterogeneity and local variations of assortativity within a network and exhibit, in a variety of empirical data, rich mixing patterns that would be obscured by summarizing assortativity with a single statistic. Assortative mixing in networks is the tendency for nodes with the same attributes, or metadata, to link to each other. It is a property often found in social networks, manifesting as a higher tendency of links occurring between people of the same age, race, or political belief. Quantifying the level of assortativity or disassortativity (the preference of linking to nodes with different attributes) can shed light on the organization of complex networks. It is common practice to measure the level of assortativity according to the assortativity coefficient, or modularity in the case of categorical metadata. This global value is the average level of assortativity across the network and may not be a representative statistic when mixing patterns are heterogeneous. For example, a social network spanning the globe may exhibit local differences in mixing patterns as a consequence of differences in cultural norms. Here, we introduce an approach to localize this global measure so that we can describe the assortativity, across multiple scales, at the node level. Consequently, we are able to capture and qualitatively evaluate the distribution of mixing patterns in the network. We find that, for many real-world networks, the distribution of assortativity is skewed, overdispersed, and multimodal. Our method provides a clearer lens through which we can more closely examine mixing patterns in networks.
Transcriptome Analysis Reveals Increases in Visceral Lipogenesis and Storage and Activation of the Antigen Processing and Presentation Pathway during the Mouth-Opening Stage in Zebrafish Larvae

The larval phase of the fish life cycle has the highest mortality, particularly during the transition from endogenous to exogenous feeding. However, the transcriptional events underlying these processes have not been fully characterized. To understand the molecular mechanisms underlying mouth-opening acclimation, RNA-seq was used to investigate the transcriptional profiles of the endogenous feeding, mixed feeding and exogenous feeding stages of zebrafish larvae. Differential expression analysis showed 2172 up-regulated and 2313 down-regulated genes during this stage. Genes associated with the assimilation of exogenous nutrients such as the arachidonic acid metabolism, linoleic acid metabolism, fat digestion and absorption, and lipogenesis were activated significantly, whereas dissimilation including the cell cycle, homologous recombination, and fatty acid metabolism were inhibited, indicating a physiological switch for energy storage occurred during the mouth-opening stage. Moreover, the immune recognition involved in the antigen processing and presentation pathway was activated and nutritional supply seemed to be required in this event confirmed by qPCR. These results suggested the energy utilization during the mouth-opening stage is more tended to be reserved or used for some important demands, such as activity regulation, immune defense, and lipid deposition, instead of rapid growth. The findings of this study are important for understanding the physiological switches during the mouth-opening stage.