Miracle-dz/UniFAR-FTUs huggingface dataset:
Hard cornification in reptilian epidermis in comparison to cornification in mammalian epidermis

Abstract: The structure of reptilian hard (beta)‐keratins, their nucleotide and amino acid sequence, and the organization of their genes are presented. These 13–19 kDa proteins are basic, rich in glycine, proline and serine, and different from cytokeratins. Their mRNAs are expressed in beta‐cells. The central part of beta‐keratins (this region has been previously termed ‘core‐box’ and is peculiar of all sauropsid proteins) is composed of two beta‐folded regions and shows a high identity with avian beta‐keratins. This central part present in all beta‐keratins, including feather keratins, is the site of polymerization to build the framework of beta‐keratin filaments. Beta‐keratins appear cytokeratin‐associated proteins. Their central region might have originated in an ancestral glycine‐rich protein present in stem reptiles from which beta‐keratins evolved and diversified into reptiles and birds. Stem reptiles of the Carboniferous period might have possessed glycine‐rich proteins derived from exons/domains corresponding to the variable, glycine‐rich region of cytokeratins. Beta‐keratins might have derived from a gene coding for small glycine‐rich keratin‐associated proteins. The glycine‐rich regions evolved differently in the lineage leading to modern reptiles and birds versus that leading to mammals. In the reptilian lineage some amino acid regions produced by point mutations and amino acid changes might have given rise to originate the central beta‐pleated region. The latter allowed the formation of filamentous proteins (beta‐keratins) associated with intermediate filament keratins and replaced them in beta‐keratin cells. In the mammalian lineage no beta‐pleated region was generated in their matrix proteins, the glycine‐rich keratin‐associated proteins. The latter evolved as glycine‐tyrosine‐rich, sulphur‐rich, and ultra‐sulphur‐rich proteins that are used for building hairs, horns and nails.
Peripherally Generated Foxp3+ Regulatory T Cells Mediate the Immunomodulatory Effects of IVIg in Allergic Airways Disease

IVIg is widely used as an immunomodulatory therapy. We have recently demonstrated that IVIg protects against airway hyperresponsiveness (AHR) and inflammation in mouse models of allergic airways disease (AAD), associated with induction of Foxp3+ regulatory T cells (Treg). Using mice carrying a DTR/EGFP transgene under the control of the Foxp3 promoter (DEREG mice), we demonstrate in this study that IVIg generates a de novo population of peripheral Treg (pTreg) in the absence of endogenous Treg. IVIg-generated pTreg were sufficient for inhibition of OVA-induced AHR in an Ag-driven murine model of AAD. In the absence of endogenous Treg, IVIg failed to confer protection against AHR and airway inflammation. Adoptive transfer of purified IVIg-generated pTreg prior to Ag challenge effectively prevented airway inflammation and AHR in an Ag-specific manner. Microarray gene expression profiling of IVIg-generated pTreg revealed upregulation of genes associated with cell cycle, chromatin, cytoskeleton/motility, immunity, and apoptosis. These data demonstrate the importance of Treg in regulating AAD and show that IVIg-generated pTreg are necessary and sufficient for inhibition of allergen-induced AAD. The ability of IVIg to generate pure populations of highly Ag-specific pTreg represents a new avenue to study pTreg, the cross-talk between humoral and cellular immunity, and regulation of the inflammatory response to Ags.
Association between apathy/depression and executive function in patients with Alzheimer's disease

ABSTRACT Background: Apathy and depression may be strongly associated with executive dysfunction in Alzheimer's disease (AD). The Frontal Assessment Battery (FAB) is an instrument for assessing executive function. The dual task paradigm is also useful for assessing divided attention. However, the association between apathy/depression and these tasks is unclear. Methods: Both the FAB and the dual task were used to evaluate AD patients. A two-way analysis of variance was then conducted between the FAB and dual task results and the absence versus the presence of depression or the absence versus the presence of apathy. Results: Of 88 patients with AD, 26 had both apathy and depression, 26 had depression only, 18 had apathy only, and 18 had neither. Total FAB scores and dual task scores differed significantly between the AD patients with depression and those without depression; the scores were also different between those with apathy and those without apathy. Also, a significant interaction between depression and apathy was noted for the total FAB and dual task scores. Conclusions: The deficits in the total FAB and dual task scores were larger in AD patients with both apathy and depression compared with patients with either apathy or depression alone. AD patients with both symptoms may have greater deficits in frontal lobe function relative to AD patients with either apathy or depression alone.
Structures of Shikimate Dehydrogenase AroE and Its Paralog YdiB

Shikimate dehydrogenase catalyzes the fourth step of the shikimate pathway, the essential route for the biosynthesis of aromatic compounds in plants and microorganisms. Absent in metazoans, this pathway is an attractive target for nontoxic herbicides and drugs. Escherichia coli expresses two shikimate dehydrogenase paralogs, the NADP-specific AroE and a putative enzyme YdiB. Here we characterize YdiB as a dual specificity quinate/shikimate dehydrogenase that utilizes either NAD or NADP as a cofactor. Structures of AroE and YdiB with bound cofactors were determined at 1.5 and 2.5 Å resolution, respectively. Both enzymes display a similar architecture with two α/β domains separated by a wide cleft. Comparison of their dinucleotide-binding domains reveals the molecular basis for cofactor specificity. Independent molecules display conformational flexibility suggesting that a switch between open and closed conformations occurs upon substrate binding. Sequence analysis and structural comparison led us to propose the catalytic machinery and a model for 3-dehydroshikimate recognition. Furthermore, we discuss the evolutionary and metabolic implications of the presence of two shikimate dehydrogenases in E. coli and other organisms.
A Cascade-Parallel Based Noise De-Embedding Technique for RF Modeling of CMOS Device

In this letter, a unique cascade-parallel based noise de-embedding technique is presented for on-wafer device characterization and modeling. It utilizes two fully shielded THRU line structures and one OPEN structure that enable simultaneously de-embedding of series contact resistance, forward coupling and distributed parasitics of interconnect. Thus, it is more suitable for RF/millimeter wave noise characterization of lossy CMOS devices as compared to conventional lumped and cascade based de-embedding techniques. The proposed noise de-embedding technique is verified on both zero length THRU and OPEN devices. It demonstrates a better high frequency de-embedding performance than existing cascade based techniques by showing 1 dB improvement in predicted NFmin of 0.13 μm CMOS devices at 60 GHz. This is consistent with the further validation result on the de-embedded gain performance of the transistor.
Characterization of actin- and lipid-binding domains in severin, a Ca(2+)-dependent F-actin fragmenting protein.

Severin is a Ca(2+)-activated actin-binding protein that nucleates actin assembly and severs and caps the fast growing ends of actin filaments. It consists of three highly conserved domains. To investigate the domain structure of severin, we constructed genetically the N-terminal domain 1, the middle domain 2, and the tandem domains 2 + 3. Their interaction with actin, Ca2+, and lipids was characterized. Domain 1 contains the F-actin capping and a Ca(2+)-binding site [Eichinger, L. , Noegel, A. A. , & Schleicher, M. (1991) J. Cell Biol. 112, 665-676]. Binding of domain 2 to actin filaments was Ca(2+)-dependent and saturated at a 1:1 molar ratio. In the presence of Ca2+, about 1.5 mol of domains 2 + 3 bound per mole of F-actin subunit. Scatchard analysis gave a Kd of 18 microM for the interaction of domain 2 with F-actin subunits and a Kd of 1.6 microM for domains 2 + 3. Low-shear viscometry, electron microscopy, and low-speed sedimentation assays showed that domains 2 + 3 induced bundling of actin filaments. The influence of PIP2 micelles on the different activities of severin was assayed using native severin and N- and C-terminally truncated fragments. Severin contains at least two PIP2-binding sites since the activities of the two nonoverlapping severin fragments domain 1 and domains 2 + 3 were inhibited by PIP2. The specificity of severin-phospholipid interaction was investigated by studying the regulation of native severin by PIP2 and other pure or mixed phospholipids. (ABSTRACT TRUNCATED AT 250 WORDS)
Watermarking scheme based on game of life cellular automaton

Chaotic maps improve the security level of watermarking scheme due to its complex characteristics. The security is required to prevent unauthorized modifications. In this paper, a highly security based watermarking scheme for image authentication has been proposed. The two dimensional Arnold's cat map, logistic map and 2D Game of Life cellular automata provide the security scheme. Arnold cat map has been used to scramble the original image and logistic map and Game of Life automaton have been used to scramble the binary watermark image. The scrambled watermark image is embedded into the least significant bit plane of scrambled original image. The proposed method uses multiple chaotic maps which results in large key space. Therefore, the proposed method is capable of preventing unauthorized modifications. Experimental results of the proposed method is compared with other three existing methods which are chaos based watermarking schemes. Experimental results show that the proposed method outperforms than other existing methods.
Combined Molecular and Conventional Analyses of Nitrifying Bacterium Diversity in Activated Sludge: Nitrosococcus mobilis and Nitrospira-Like Bacteria as Dominant Populations

ABSTRACT The ammonia-oxidizing and nitrite-oxidizing bacterial populations occurring in the nitrifying activated sludge of an industrial wastewater treatment plant receiving sewage with high ammonia concentrations were studied by use of a polyphasic approach. In situ hybridization with a set of hierarchical 16S rRNA-targeted probes for ammonia-oxidizing bacteria revealed the dominance ofNitrosococcus mobilis-like bacteria. The phylogenetic affiliation suggested by fluorescent in situ hybridization (FISH) was confirmed by isolation of N. mobilis as the numerically dominant ammonia oxidizer and subsequent comparative 16S rRNA gene (rDNA) sequence and DNA-DNA hybridization analyses. For molecular fine-scale analysis of the ammonia-oxidizing population, a partial stretch of the gene encoding the active-site polypeptide of ammonia monooxygenase (amoA) was amplified from total DNA extracted from ammonia oxidizer isolates and from activated sludge. However, comparative sequence analysis of 13 amoA clone sequences from activated sludge demonstrated that these sequences were highly similar to each other and to the corresponding amoA gene fragments ofNitrosomonas europaea Nm50 and the N. mobilisisolate. The unexpected high sequence similarity between theamoA gene fragments of the N. mobilisisolate and N. europaea indicates a possible lateral gene transfer event. Although a Nitrobacter strain was isolated, members of the nitrite-oxidizing genus Nitrobacter were not detectable in the activated sludge by in situ hybridization. Therefore, we used the rRNA approach to investigate the abundance of other well-known nitrite-oxidizing bacterial genera. Three different methods were used for DNA extraction from the activated sludge. For each DNA preparation, almost full-length genes encoding small-subunit rRNA were separately amplified and used to generate three 16S rDNA libraries. By comparative sequence analysis, 2 of 60 randomly selected clones could be assigned to the nitrite-oxidizing bacteria of the genusNitrospira. Based on these clone sequences, a specific 16S rRNA-targeted probe was developed. FISH of the activated sludge with this probe demonstrated that Nitrospira-like bacteria were present in significant numbers (9% of the total bacterial counts) and frequently occurred in coaggregated microcolonies with N. mobilis.
Up-Down Determination of the 90% Effective Dose of Phenylephrine for the Treatment of Spinal Anesthesia-Induced Hypotension in Parturients Undergoing Cesarean Delivery

INTRODUCTION: Hypotension frequently complicates spinal anesthesia for cesarean delivery, and vasopressors are the mainstay for treatment. The most effective dose of phenylephrine for the treatment of spinal anesthesia-induced hypotension has not been estimated. METHODS: Healthy nonlaboring women undergoing a cesarean delivery were recruited. All women received spinal anesthesia using hyperbaric bupivacaine 12 mg with fentanyl and morphine. Each subject received an IV crystalloid fluid bolus before and at the time of initiation of spinal anesthesia (preload and coload). An up-down sequential allocation method using the biased-coin design was used to estimate the 90% effective dose (ED90) of phenylephrine. The assigned phenylephrine dose was based on the response of the preceding subject. If the systolic blood pressure (SBP) decreased >20% of baseline (i.e., SBP 20%) or to an SBP <90 mm Hg, the assigned dose of phenylephrine was administered. If the SBP returned to within 20% of baseline or ≥90 mm Hg within 1 min, this was considered a success, otherwise it was a failure. The initial dose of phenylephrine was 100 μg. The ED90 with 95% confidence intervals (CIs) was calculated using the maximum likelihood estimation and Firth logistic regression. RESULTS: Sixty-nine subjects were screened to participate, of whom 66 subjects consented. Forty-five of the enrolled subjects experienced spinal anesthesia-induced hypotension and received a blinded dose of phenylephrine. Those subjects who developed hypotension received doses of phenylephrine between 80 and 180 μg. No subjects experienced hypertension. Determined with the maximum likelihood estimation method, the ED90 of phenylephrine was 147 μg (95% CI, 98–222 μg). With Firth regression, the probability of a successful response at 150 μg is 90.5% (95% CI, 66.0%–99.0%). CONCLUSION: In this study, we estimated that the ED90 of phenylephrine required to treat spinal anesthesia-induced hypotension in cesarean delivery is approximately 150 μg.
Determining the Invariant Structure Elements of the HIV-1 Variable V3 Loops: Insight into the HIV-MN and HIV-Haiti Isolates

Abstract The computer approaches that combined the 3D protein structure modeling with the mathematical statistics methods were used to compute the NMR-based 3D structures of the HIV-1 gp120 V3 loop for the HIV-MN and HIV-Haiti isolates in water as well as to compare their conformational characteristics with the purpose of determining the structure elements common for the two virus modifications. As a result, the variability of the amino acid sequence was found to stimulate the considerable structural rearrangements of the V3 loop. However, despite this fact, one functionally crucial stretch of V3 and a greater portion of its residues were shown to preserve the conformations in the viral strains of interest. To reveal the structural motifs and individual amino acids giving rise to the close conformations in the HIV-MN and HIV-Haiti V3 loops regardless of the sequence and environment variability, the simulated structures were collated with those deciphered previously in terms of NMR data in a water/trifluoroethanol mixed solvent. The structure elements and single residues of V3 residing in its biologically significant sites and keeping the conformations in all of the cases at question are considered to be the promising targets for anti-AIDS drugs studies. In this context, the structurally inflexible motifs of V3 presenting the weak units in the virus protection system may be utilized as the most convenient landing-places for molecular docking of the V3 loop and ligand structures followed by selecting chemical compounds suitable as a basis for the design of safe and effective antiviral agents.
Using Simulation to Instruct Emergency Medicine Residents in Cognitive Forcing Strategies

Purpose. Recent literature defines certain cognitive errors that emergency physicians will likely encounter. The authors have utilized simulation and debriefing to teach the concepts of metacognition and error avoidance. Method. The authors conducted a qualitative study of an educational intervention at Lehigh Valley Hospital during academic year 2002–03. Fifteen emergency medicine residents—eight from postgraduate year three (PGY3) and seven from postgraduate year two (PGY2)—experienced a difficult simulator lab scenario designed to lead them into a cognitive error trap. The debriefing was a PowerPoint with audio format CD-ROM with a didactic on succinylcholine (15 minutes) and cognitive forcing strategies (30 minutes). After debriefing, residents were interviewed by an ethnographer with an 11-question (15-minute) interview and completed an eight-question written survey. Results. The residents ranked this experience second only to direct patient care for educational effectiveness. Survey results (Likert scale, 1 = disagree completely to 5 = agree completely) included “Improved my ability to use succinylcholine” (mean = 4.73), “Improved my ability to diagnose and treat hyperkalemia” (mean = 4.6), and “Cognitive forcing strategies is a useful educational effort” (mean = 4.33). The major interview themes that evolved were that the simulation lab was a positive experience; succinylcholine knowledge was gained; mistakes caused reflection/motivation; the lab was stressful; attending feedback was desired; the lab was realistic; and cognitive forcing strategies were discussed. When asked what they learned, more of the PGY3s commented on cognitive strategies or heuristic techniques (six out of eight), whereas the PGY2s commented on knowledge gained about succinylcholine (five out of seven) and only one PGY2 mentioned cognitive strategies. Conclusion. Pilot data suggest that metacognitive strategies can be taught to residents, though they may be better understood by upper-level residents.
Combined GnRH-agonist/gonadotrophin stimulation for in-vitro fertilization.

The occurrence of a premature luteinizing hormone (LH) surge during gonadotrophin stimulation for in-vitro fertilization leads to cancellation of the cycle. Moreover, insufficient follicular maturation is often caused by elevated basal gonadotrophin levels. Therefore, the gonadotrophin-releasing hormone (GnRH) agonist, D-Trp-6-LHRH, was applied to patients exhibiting premature LH surges, hyperandrogenaemia or incipient premature menopause. A total of 119 cycles were treated using a long-acting versus a short-acting GnRH agonistic analogue. In protocol 1, patients received daily s.c. injections of 100-500 micrograms of a short-acting compound. In protocol 2, a long-acting bolus of 3.2 mg was given i.m. Concomitant human gonadotrophin stimulation was started in protocol 1 after clinical and biochemical evidence of pituitary suppression and in protocol 2 after a fixed suppression interval of 14 days. In protocol 1, higher oestrogen levels were reached with more oocytes harvested. The pregnancy rate per transfer was increased from 3.5 to 18%, with most pregnancies occurring with protocol 1. The cancellation rate of 13.4% was mainly due to insufficient follicular development in patients in whom premature menopause was suspected. Hyperandrogenaemic patients with an elevated LH/FSH ratio exhibited the best follicular recruitment with the highest pregnancy rate of 25% per transfer. Thus, combined GnRH-agonist/gonadotrophin stimulation offers a causal treatment for patients susceptible to premature LH surges and for hyperandrogenaemic patients.
On the domain‐specificity of the visual and non‐visual face‐selective regions

What happens in our brains when we see a face? The neural mechanisms of face processing – namely, the face‐selective regions – have been extensively explored. Research has traditionally focused on visual cortex face‐regions; more recently, the role of face‐regions outside the visual cortex (i.e., non‐visual‐cortex face‐regions) has been acknowledged as well. The major quest today is to reveal the functional role of each this region in face processing. To make progress in this direction, it is essential to understand the extent to which the face‐regions, and particularly the non‐visual‐cortex face‐regions, process only faces (i.e., face‐specific, domain‐specific processing) or rather are involved in a more domain‐general cognitive processing. In the current functional MRI study, we systematically examined the activity of the whole face‐network during face‐unrelated reading task (i.e., written meaningful sentences with content unrelated to faces/people and non‐words). We found that the non‐visual‐cortex (i.e., right lateral prefrontal cortex and posterior superior temporal sulcus), but not the visual cortex face‐regions, responded significantly stronger to sentences than to non‐words. In general, some degree of sentence selectivity was found in all non‐visual‐cortex cortex. Present result highlights the possibility that the processing in the non‐visual‐cortex face‐selective regions might not be exclusively face‐specific, but rather more or even fully domain‐general. In this paper, we illustrate how the knowledge about domain‐general processing in face‐regions can help to advance our general understanding of face processing mechanisms. Our results therefore suggest that the problem of face processing should be approached in the broader scope of cognition in general.
Investigation of Methods for Reduction of Power Fluctuations Generated From Large Grid-Connected Photovoltaic Systems

Photovoltaic (PV) systems are presently allowed to inject into the grid all the power they can generate. However, in the near future, utilities are expected to impose additional regulations and restrictions on the power being injected by large centralized PV systems because of their possible adverse impacts. One of the main issues associated with large PV systems is the fluctuation of their output power. These fluctuations can negatively impact the performance of the electric networks to which these systems are connected, especially if the penetration levels of these systems are high. Moreover, the fluctuations in the power of PV systems make it difficult to predict their output, and thus, to consider them when scheduling the generating units in the network. The main objective of this paper is to investigate some methods that can be used to reduce the fluctuations in the power generated from a large customer-owned PV system, in the order of megawatts. This paper focuses on three methods: 1) the use of battery storage systems; 2) the use of dump loads; and 3) curtailment of the generated power by operating the power-conditioning unit of the PV system below the maximum power point. The emphasis in the analysis presented in this paper is on investigating the impacts of implementing these methods on the economical benefits that the PV system owner gains. To estimate the maximum revenues gained by the system owner, an linear programming optimization problem is formulated and solved. Moreover, the effect of varying different parameters of the problem is investigated through sensitivity analysis.