Miracle-dz/UniFAR-FTUs huggingface dataset:
Nanowire Networks: Three‐Dimensional SnO2 Nanowire Networks for Multifunctional Applications: From High‐Temperature Stretchable Ceramics to Ultraresponsive Sensors (Adv. Electron. Mater. 8/2015)

Stretchable three-dimensional ceramic networks constructed from quasi-onedimensional metal oxide nanostructures are versatile candidates for using nanoscopic structures in everyday technologies. The growth of such networks built from SnO 2 nanostructures is realized by a simple flame transport synthesis. As reported by Lorenz Kienle, Rainer Adelung, Yogendra Kumar Mishra, and co-workers in article number 1500081, these grown SnO 2 nanostructures with unique structural defects can be used for sensing applications.
Reduced insulin clearance and lower insulin-degrading enzyme expression in the liver might contribute to the thrifty phenotype of protein-restricted mice

Nutrient restriction during the early stages of life usually leads to alterations in glucose homeostasis, mainly insulin secretion and sensitivity, increasing the risk of metabolic disorders in adulthood. Despite growing evidence regarding the importance of insulin clearance during glucose homeostasis in health and disease, no information exists about this process in malnourished animals. Thus, in the present study, we aimed to determine the effect of a nutrient-restricted diet on insulin clearance using a model in which 30-d-old C57BL/6 mice were exposed to a protein-restricted diet for 14 weeks. After this period, we evaluated many metabolic variables and extracted pancreatic islet, liver, gastrocnemius muscle (GCK) and white adipose tissue samples from the control (normal-protein diet) and restricted (low-protein diet, LP) mice. Insulin concentrations were determined using RIA and protein expression and phosphorylation by Western blot analysis. The LP mice exhibited lower body weight, glycaemia, and insulinaemia, increased glucose tolerance and altered insulin dynamics after the glucose challenge. The improved glucose tolerance could partially be explained by an increase in insulin sensitivity through the phosphorylation of the insulin receptor/protein kinase B and AMP-activated protein kinase/acetyl-CoA carboxylase in the liver, whereas the changes in insulin dynamics could be attributed to reduced insulin secretion coupled with reduced insulin clearance and lower insulin-degrading enzyme (IDE) expression in the liver and GCK. In summary, protein-restricted mice not only produce and secrete less insulin, but also remove and degrade less insulin. This phenomenon has the double benefit of sparing insulin while prolonging and potentiating its effects, probably due to the lower expression of IDE in the liver, possibly with long-term consequences.
Promotion of physical activity in a developing country: The Agita São Paulo experience

Abstract The purpose of this paper is to present key points of an intervention programme (Agita São Paulo Program) to promote physical activity in a developing country. Agita is a multi-level, community-wide intervention designed to increase knowledge about the benefits and the level of physical activity in a mega-population of 34 million inhabitants of São Paulo State, Brazil. The main message was taken from the Centers for Disease Control/American College of Sports Medicine (CDC/ACSM) recommendation that: ‘everyone should accumulate at least 30 minutes of physical activity, on most days of the weeks, of moderate intensity, in one single or in multiple sessions’. Activities were encouraged in three settings: home, transport and leisure time. Focus groups were students from elementary schools through to college, white and blue collar workers, and elderly people. Innovative aspects included: (1) a research centre leading the process, (2) scientific and institutional partnerships (over 160 groups), (3) a feasible approach – the ‘one-step-ahead’ model, (4) empowerment, (5) inclusion, (6) non-paid media, (7) social marketing, and (8) culture-linked. Data were obtained from 645 random, home-based questionnaires over four years – stratified by sex, age, education and socio-economic level. These data show that the Agita message reached 55.7% of the population, and among these, 23.1% knew the main message. Recall of Agita and knowledge of its purpose were well distributed among different socioeconomic levels, being known by 67% of the most educated. The prevalence of people reaching the recommendation was 54.8% (men 48.7%, women 61%); and risk of being sedentary was quite smaller among those who knew the Agita message (7.1%) compared with those who did not know (13.1%). In conclusion, based upon the Agita São Paulo experience, it appears that a multi-level, community-wide intervention to promote physical activity may obtain good results if the model contains the items listed above.
Reduced Gaze Following and Attention to Heads when Viewing a "Live" Social Scene

Social stimuli are known to both attract and direct our attention, but most research on social attention has been conducted in highly controlled laboratory settings lacking in social context. This study examined the role of social context on viewing behaviour of participants whilst they watched a dynamic social scene, under three different conditions. In two social groups, participants believed they were watching a live webcam of other participants. The socially-engaged group believed they would later complete a group task with the people in the video, whilst the non-engaged group believed they would not meet the people in the scene. In a third condition, participants simply free-viewed the same video with the knowledge that it was pre-recorded, with no suggestion of a later interaction. Results demonstrated that the social context in which the stimulus was viewed significantly influenced viewing behaviour. Specifically, participants in the social conditions allocated less visual attention towards the heads of the actors in the scene and followed their gaze less than those in the free-viewing group. These findings suggest that by underestimating the impact of social context in social attention, researchers risk coming to inaccurate conclusions about how we attend to others in the real world.
Retention of a cell adhesion complex at the paranodal junction requires the cytoplasmic region of Caspr

An axonal complex of cell adhesion molecules consisting of Caspr and contactin has been found to be essential for the generation of the paranodal axo-glial junctions flanking the nodes of Ranvier. Here we report that although the extracellular region of Caspr was sufficient for directing it to the paranodes in transgenic mice, retention of the Caspr–contactin complex at the junction depended on the presence of an intact cytoplasmic domain of Caspr. Using immunoelectron microscopy, we found that a Caspr mutant lacking its intracellular domain was often found within the axon instead of the junctional axolemma. We further show that a short sequence in the cytoplasmic domain of Caspr mediated its binding to the cytoskeleton-associated protein 4.1B. Clustering of contactin on the cell surface induced coclustering of Caspr and immobilized protein 4.1B at the plasma membrane. Furthermore, deletion of the protein 4.1B binding site accelerated the internalization of a Caspr–contactin chimera from the cell surface. These results suggest that Caspr serves as a “transmembrane scaffold” that stabilizes the Caspr/contactin adhesion complex at the paranodal junction by connecting it to cytoskeletal components within the axon.
Satellite monitoring of disturbances in Arctic ecosystems

This study explores the capability of satellite remote sensing to detect relatively rapid changes of vegetation cover in northern Fennoscandian regions in response to disturbance more generally, and insect defoliation damage in particular. The data used is a long term time series of leaf area index (LAI) at 8 Km resolution derived from the Advanced Very High Resolution Radiometer (AVHRR) between 1982 and 2006, developed to be structurally consistent with the Moderate Resolution Imaging Spectrometer (MODIS) record. The study explores the potential of frequentist traditional statistics to detect disturbances at this coarse spatial resolution over the 25 years time series, and outlines the possibilities that Bayesian methods offer to improve the detection by including prior information on the profile of such disturbance events.
Regulated and aberrant glycosylation modulate cardiac electrical signaling

Millions afflicted with Chagas disease and other disorders of aberrant glycosylation suffer symptoms consistent with altered electrical signaling such as arrhythmias, decreased neuronal conduction velocity, and hyporeflexia. Cardiac, neuronal, and muscle electrical signaling is controlled and modulated by changes in voltage-gated ion channel activity that occur through physiological and pathological processes such as development, epilepsy, and cardiomyopathy. Glycans attached to ion channels alter channel activity through isoform-specific mechanisms. Here we show that regulated and aberrant glycosylation modulate cardiac ion channel activity and electrical signaling through a cell-specific mechanism. Data show that nearly half of 239 glycosylation-associated genes (glycogenes) were significantly differentially expressed among neonatal and adult atrial and ventricular myocytes. The N-glycan structures produced among cardiomyocyte types were markedly variable. Thus, the cardiac glycome, defined as the complete set of glycan structures produced in the heart, is remodeled. One glycogene, ST8sia2, a polysialyltransferase, is expressed only in the neonatal atrium. Cardiomyocyte electrical signaling was compared in control and ST8sia2(−/−) neonatal atrial and ventricular myocytes. Action potential waveforms and gating of less sialylated voltage-gated Na+ channels were altered consistently in ST8sia2(−/−) atrial myocytes. ST8sia2 expression had no effect on ventricular myocyte excitability. Thus, the regulated (between atrium and ventricle) and aberrant (knockout in the neonatal atrium) expression of a single glycogene was sufficient to modulate cardiomyocyte excitability. A mechanism is described by which cardiac function is controlled and modulated through physiological and pathological processes that involve regulated and aberrant glycosylation.
Time-Resolved Optical and Terahertz Characterization of Hg-Based High-Temperature Superconductors

This thesis presents a detailed study of structural and optoelectronic properties of Hgbased high temperature superconductors (HBCCO). The optical and terahertz characterization techniques used include time-resolved pump-probe spectroscopy, photoimpedance measurements, transmission terahertz time-domain spectroscopy (THz-TDS), and optical-pump and THz-probe (OPTP) spectroscopy. We used atomic force microscopy, X-ray diffraction, and scanning electron microscopy to characterize the surfaces and structures of the studied HBCCO thin films and microbridges. In optical pump-probe spectroscopy, we measured the temperatureand powerdependent transient reflectivity signals. In the superconducting state, the amplitude and rise time of the signals followed BCS-anisotropic energy gap dependence. The rise time in the normal state was faster than that in the superconducting state. The relaxation time of the superconducting components just below Tc followed 1/∆(t) dependence since it was decided by the phonon anharmonic decay process. Below 0.8 Tc, instead of being limited by the “phonon bottleneck” effect, typical for conventional superconductors, the relaxation time was decided by the quasiparticle recombination process, and was in a single-picosecond range. The latter shows that HBCCO is a good candidate for ultrafast photodetectors.
Transcription and decay of the lac messenger: role of an intergenic terminator

Prior work has indicated that the polycistronic lacZYA mRNA of Escherichia coli is cleaved during decay at approximately intergenic sites (L. W. Lim and D. Kennell, J. Mol. Biol. 135: 369-390, 1979). In this work, we characterized the products by using probes specific for the different cistrons. This analysis indicated that six lac mRNA species are present in the following order of decreasing abundance: lacZ, -A, -ZYA, -ZY, -YA, and -Y. Very little lacYA and lacY mRNAs were present, whereas in cells induced to steady state, there was 10 times more lacZ than lacZYA mRNA. The lacZ mRNA appeared as a discrete species extending to a site in the lacZ-Y intergenic space (ca. residue 3150). This site is just distal to a potential rho-independent termination sequence. We examined the function of this sequence to determine whether it contributes to the distribution of the mRNAs. Although the termination sequence was shown to function in vitro, when it was recloned into an expression vector, no termination was seen in vivo. Moreover, direct examination of the kinetics of lac messenger synthesis revealed that after initiation, most transcription continued to the end of the operon. We conclude that during normal growth, the operon is transcribed in its entirety and that the individual lac mRNAs are formed by cleavage. These results confirm earlier work implying that the lac operon is transcribed in its entirety but are in conflict with several recent reports suggesting that internal termination occurs. Our findings indicate that the natural polarity of the operon (lacZ is expressed sixfold more strongly than lacA) is based on posttranslational effects and not on polarity of transcription.
Demand-driven structural testing with dynamic instrumentation

Producing reliable and robust software has become one of the most important software development concerns. Testing is a process by which software quality can be assured through the collection of information. While testing can improve software reliability, current tools typically are inflexible and have high overheads, making it challenging to test large software projects. In this paper, we describe a new scalable and flexible framework for testing programs with a novel demand-driven approach based on execution paths to implement test coverage. This technique uses dynamic instrumentation on the binary code that can be inserted and removed on-the-fly to keep performance and memory overheads low. We describe and evaluate implementations of the framework for branch, node and def-use testing of Java programs. Experimental results for branch testing show that our approach has, on average, a 1.6 speed up over static instrumentation and also uses less memory.
Specificity, duplex degradation and subcellular localization of antagomirs

MicroRNAs (miRNAs) are an abundant class of 20–23-nt long regulators of gene expression. The study of miRNA function in mice and potential therapeutic approaches largely depend on modified oligonucleotides. We recently demonstrated silencing miRNA function in mice using chemically modified and cholesterol-conjugated RNAs termed ‘antagomirs’. Here, we further characterize the properties and function of antagomirs in mice. We demonstrate that antagomirs harbor optimized phosphorothioate modifications, require >19-nt length for highest efficiency and can discriminate between single nucleotide mismatches of the targeted miRNA. Degradation of different chemically protected miRNA/antagomir duplexes in mouse livers and localization of antagomirs in a cytosolic compartment that is distinct from processing (P)-bodies indicates a degradation mechanism independent of the RNA interference (RNAi) pathway. Finally, we show that antagomirs, although incapable of silencing miRNAs in the central nervous system (CNS) when injected systemically, efficiently target miRNAs when injected locally into the mouse cortex. Our data further validate the effectiveness of antagomirs in vivo and should facilitate future studies to silence miRNAs for functional analysis and in clinically relevant settings.
Error resilient pre/post-filtering for DCT-based block coding systems

Block coding based on the discrete cosine transform (DCT) is very popular in image and video compression. Pre/post-filtering can be attached to a DCT-based block coding system to improve coding efficiency as well as to mitigate blocking artifacts. Previously designed pre/post-filters are optimized to maximize coding efficiency solely. For image and video communication over unreliable channels, those pre/post-filters are sensitive to transmission errors. This paper addresses the problem of designing pre/post-filters which are more error resilient. Reconstruction performance is measured by how low the average reconstruction error is, and how uniformly the reconstruction error is distributed. A family of pre/post-filters is designed to provide desired tradeoffs between coding efficiency and robustness to transmission errors. Experiments show that these filtering operators can achieve superior reconstruction performance without sacrificing much coding performance.
Coupling to a cancer cell‐specific antibody potentiates tumoricidal properties of curcumin

In vitro studies have shown that curcumin, a polyphenol from the culinary component turmeric, has strong anticancer properties. However, there is no consensus on its therapeutic effect in human. Our earlier experiments involving implanted murine melanoma B16F10 cells in the neck or brain of syngeneic C57BL6 mice showed that tail vein injection of curcumin blocks formation of lesions and tumor in these mice. However, such treatment was ineffective in eliminating established tumors that already occupied ≤10% of brain volume. Possible reasons include low solubility and rapid metabolism of curcumin in vivo. To increase its efficacy, we have linked curcumin through a cleavable arm to an antibody (Ab) against the melanoma surface antigen Muc18. The antibody‐coupled curcumin was 230‐fold more effective in eliminating B16F10 cells in vitro, and in vivo, it rapidly decimated established, B16F10‐evoked brain tumors, enabling the rescued mice to live normally far beyond 90 days from implantation of cancer cells. In contrast, mice treated with Muc18 Ab alone died of brain tumor within a month. In B16F10 cells, curcumin‐Ab (adduct) treatment caused a dramatic inhibition of NF‐kB: a transcription factor that is constitutively activated in cancer cells. Furthermore, overexpression of NF‐kB in the B16F10 cells blocked adduct‐evoked stimulation of caspase‐3/7 activity. Thus, by suppressing NF‐kB, the curcumin adduct inhibits other downstream tumor‐promoting proteins, thereby eliminating the B16F10 cells. Our study submits a novel yet generally applicable strategy of converting curcumin into a potent anticancer agent and provides a mechanistic framework for its action.
Phylogenetics from paralogs

Motivation: Sequence-based phylogenetic approaches heavily rely on initial data sets to be composed of orthologous sequences only. Paralogs are treated as a dangerous nuisance that has to be detected and removed. Recent advances in mathematical phylogenetics, however, have indicated that gene duplications can also convey meaningful phylogenetic information provided orthologs and paralogs can be distinguished with a degree of certainty. Results: We demonstrate that plausible phylogenetic trees can be inferred from paralogy information only. To this end, tree-free estimates of orthology, the complement of paralogy, are first corrected to conform cographs and then translated into equivalent event-labeled gene phylogenies. A certain subset of the triples displayed by these trees translates into constraints on the species trees. While the resolution is very poor for individual gene families, we observe that genome-wide data sets are sufficient to generate fully resolved phylogenetic trees of several groups of eubacteria. The novel method introduced here relies on solving three intertwined NP-hard optimization problems: the cograph editing problem, the maximum consistent triple set problem, and the least resolved tree problem. Implemented as Integer Linear Program, paralogy-based phylogenies can be computed exactly for up to some twenty species and their complete protein complements. Availability: The ILP formulation is implemented in the Software ParaPhylo using IBM ILOG CPLEX TM Optimizer 12.6 and is freely available from http://pacosy.informatik. uni-leipzig.de/ paraphylo.
Chapter 2 Chronic Wound Colonization , Infection , and Biofilms

The term “chronic wound” is generally accepted, but yet no simple definition has been agreed upon. A mechanistic definition such as “those not following normal wound healing trajectory” have been proposed but the most common definition have been “ulcers (wounds) older than 3 months of age”. Indeed, some ulcers have been present for years. One to two percent of the population in the developed countries will experience a chronic wound in their lifetime (Gottrup 2004). It is expected that the number of chronic wounds will increase worldwide due to the increase of lifestyle diseases, such as diabetes, obesity, and cardiovascular diseases. It is estimated that 246 million people had diabetes worldwide in 2007 and this is expected to increase to 380 million people by the year 2025 (Diabetes Atlas). Chronic wounds cause a significant burden to healthcare systems as well as morbidity and mortality to mankind. In Denmark, it has been estimated that the prevalence of non-healing wounds is about 1% of the population. The prevalence and incidence of ulcers are similar to elsewhere in the industrialized world. The total expenses for treatment of wounds are estimated to be approximately 2–3% of the total budget of the health care system in Denmark (Ayello et al. 2004, Gottrup 2004) which matches the percentages from the European Community and probably United States of America as well. Notably in the United States there has been an increase of hospital stays for pressure ulcers by approximately 80% from 1993 to 2006. The 2007 Medicare data indicated that the development of a pressure ulcer increased a person’s hospital costs by $43,180. This adds up to 11 billion dollars a year in increased health care cost in the United States. According to the American Diabetes Association (ADA), 17.9 million Americans are diagnosed with either type I or type II diabetes, which is a major cause of premature mortality and disability. Additionally, it has been estimated that there are 6.2 million undiagnosed cases of diabetes in the United States. Often a chronic wound is the first manifestation of diabetes complications. Roughly 15% of people living with diabetes are estimated to
Uncoupling protein 2 haplotype does not affect human brain structure and function in a sample of community-dwelling older adults

Uncoupling protein 2 (UCP2) is a mitochondrial membrane protein that plays a role in uncoupling electron transport from adenosine triphosphate (ATP) formation. Polymorphisms of the UCP2 gene in humans affect protein expression and function and have been linked to survival into old age. Since UCP2 is expressed in several brain regions, we investigated in this study whether UCP2 polymorphisms might 1) affect occurrence of neurodegenerative or mental health disorders and 2) affect measures of brain structure and function. We used structural magnetic resonance imaging (MRI), diffusion-weighted MRI and resting-state functional MRI in the neuroimaging sub-study of the Whitehall II cohort. Data from 536 individuals aged 60 to 83 years were analyzed. No association of UCP2 polymorphisms with the occurrence of neurodegenerative disorders or grey and white matter structure or resting-state functional connectivity was observed. However, there was a significant effect on occurrence of mood disorders in men with the minor alleles of -866G>A (rs659366) and Ala55Val (rs660339)) being associated with increasing odds of lifetime occurrence of mood disorders in a dose dependent manner. This result was not accompanied by effects of UCP2 polymorphisms on brain structure and function, which might either indicate that the sample investigated here was too small and underpowered to find any significant effects, or that potential effects of UCP2 polymorphisms on the brain are too subtle to be picked up by any of the neuroimaging measures used.