Miracle-dz/UniFAR-FTUs huggingface dataset:
Estudio de los genes implicados en la degradación del ácido fenilacético en pseudomonas sp. Y2

El estudio de la degradacion de compuestos aromaticos pro los microorganismos presenta gran interes, puesto que un mayor conocimiento de esos proceso puede llega a permitir la eliminacion de contaminantes del medio ambiente, entre otras varias aplicaciones. Pseudomonas sp. Y2 es capaz de degradar el estireno, un compuesto aromatico de amplio uso industrial, hasta acido fenilacetico AFA, que es despues catabolizado completamente. Estudios previos de nuestro grupo habian conducido al aislamiento y caracterizacion de los genes sty, encargados de la conversion del estireno en AFA. En este trabajo se ha llevado a cabo el aislamiento y caracterizacion de los genes paa, implicados en la degradacion del AFA hasta intermediarios del ciclo de Krebs. Se han secuenciado dos regiones del genoma de Pseudomonas ps.Y2 denominadas paa1 y paa2, con un tamano de 20,5kb y 21,5 kb, respectivamente, que contienen dos conjuntos completos de genes capaces de catabolizar el AFA. Se ha realizado un analisis, mediante diversos programas informaticos, de las secuencias de nucleotidos obtenidas. LA region paa1 contiene un total de 18 genes, mientras que la paa2 contiene 21 genes, de los cuales cuatro no guardan relacion con el catabolismo del AFA. Tanto los genes paa1 como los paa2 son funcionales, pudiendo llevar a cabo la degradacion del AFA de forma independiente, y se ha construido un plasmido movilizable con los genes paa1, que proporciona a otras bacterias la capacidad de emplear AFA como fuente de carbono y energia. Pseudomonas sp. Y2 es capaz de degradar estireno, 2-feniletanol, 2,-feniletilamina y fenilacetaldehido tras convertir estos compuestos en AFA, lo que confirma la existencia un analisis de la expresion de los genes sty y paa bajo diversas condiciones, lo que ha permitido comprobar, entre otros datos, que el gen crc participa en la represion catabolica de los genes sty por AFA y pro medio rico LB.
Partial duplicate image retrieval using fast visual word generation technique

Content-Based Image Retrieval (CBIR), a technique which uses visual contents to search images from large scale image databases according to users interests. In most of image retrieval systems, accurate and partial relevant matching is main issue. Partial duplicate image retrieval is most complicated task as region of interest may undergo occlusion, rotation, scaling and other transformations. Retrieval of these partial duplicate images is done by exploiting spatial information of image features. In this, position and orientation of visual word will be used to generate a COP coordinate which will be used for matching. COP coordinate are robust to rotation, scaling so better matching with high precision may be obtained. Bag of Visual Words (BOW) approach is widely used in most of Image Retrieval systems and for such systems, assignment of features to their appropriate visual words is more time-consuming. Weighted tree indexing technique is based on probabilistic tree model which uses co-occurrence of visual word within local region. This method has reduced number of backtracking while maintaining the accuracy. This system uses bag of visual words approach with weighted tree technique to generate visual words and Combined Orientation and Position (COP) graph to find more accurate matches to increase accuracy of retrieved images. Experimental results on various datasets shows that mean average precision of system is increased, leading to better retrieval accuracy.
Emergence of Ciprofloxacin-Nonsusceptible Streptococcus pyogenes Isolates from Healthy Children and Pediatric Patients in Portugal

ABSTRACT We describe 66 ciprofloxacin-nonsusceptible Streptococcus pyogenes isolates recovered from colonized and infected children. The ParC S79A substitution was frequent and associated with the emm6/sequence type 382 (emm6/ST382) lineage. The ParC D83G substitution was detected in two isolates (emm5/ST99 and emm28/ST52 lineages). One isolate (emm89/ST101) had no quinolone resistance-determining region codon substitutions or other resistance mechanisms. Five of 66 isolates were levofloxacin resistant. Although fluoroquinolones are not used in children, they may be putative disseminators of fluoroquinolone-nonsusceptible strains in the community.
Discriminatory power of typing schemes based on Simpson's index of diversity for Neisseria gonorrhoeae

Simpson's index of diversity was used to produce a single numerical value to compare the abilities of single or combined typing schemes to discriminate between unrelated isolates. This calculation was used to compare the discriminating power of auxotype and serovar determination and plasmid content analysis, either singly or in combination, for Neisseria gonorrhoeae isolates having different antimicrobial susceptibilities (i.e., antibiotic-susceptible isolates and those that produce penicillinase, carry plasmid-mediated resistance to tetracycline, have chromosomally mediated penicillin resistance, or both produce penicillinase and carry plasmid-mediated resistance to tetracycline). Plasmid content analysis and auxotype determination produced the lowest level of discrimination, while a combination of auxotype and serovar typing schemes generally provided higher levels of discrimination. Addition of plasmid content analysis to auxotype and serovar typing provided additional discrimination only with penicillinase-producing isolates. For isolates that carried plasmid-mediated resistance to tetracycline, isolates that were tetracycline resistant, isolates that both produced penicillinase and carried plasmid-mediated resistance to tetracycline, or isolates that had chromosomally mediated penicillin resistance, none of the typing methods produced high discriminatory indices, indicating that these isolates are probably derived from relatively few clones.
Isolation of Neuromelanin Granules

Neuromelanin granules are pigmented organelles in the human midbrain that give name to a brain area, substantia nigra pars compacta, which macroscopically appears as a dark brown region in the midbrain due to the insoluble pigment neuromelanin. The substantia nigra pars compacta massively degenerates in Parkinson's disease and gives rise to severely disabling movement symptoms. It has been suggested that neuromelanin granules play an important role in the neurodegenerative events in Parkinson's disease: redox‐active iron is bound to neuromelanin and thereby retained within this compartment, but in Parkinson's disease it is thought to be increasingly released into the cytosol, promoting oxidative stress. This unit includes a methodological workflow for the isolation of neuromelanin granules from the human midbrain. This top‐down approach (describes an approach that reduces the complexity of the sample stepwise from the level of tissue to cell, and from cell to organelle) encompasses the organelle isolation by sequential density gradient centrifugation and the assessment of the isolation efficacy by western blotting. Curr. Protoc. Cell Biol. 41:3.31. 1‐3.31. 15. © 2008 by John Wiley & Sons, Inc.
Effects of Ramadan fasting on serum low-density and high-density lipoprotein-cholesterol concentrations.

BACKGROUND The aim of the study was to determine the status of low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in human subjects during Ramadan fasting. Fasting during Ramadan (one month of food and water intake restricted only to night hours) is a religious obligation for Muslims. There are biochemical effects of changes in lifestyle during Ramadan. This report is a study of the effects of Ramadan fasting on the serum LDL-cholesterol and HDL-cholesterol concentrations. SUBJECTS AND METHODS The study group consisted of 83 volunteers comprising 57 males (aged 21-55, mean 34.25+/-9.81 years) and 26 females (aged 20-58, mean 34.58+/-8.94 years). A regimen of one month of food and water intake restricted only to night hours was followed by the subjects. Subjects were evaluated one week before Ramadan fasting (pre-RF), two weeks after the start of Ramadan fasting (mid-RF), and at the fourth week of Ramadan fasting (end-RF). Serum specimens were obtained from subjects during daylight hours. Measurement of LDL-cholesterol and HDL-cholesterol were performed using a spectrophotometer. RESULTS Our results showed statistically significant reduction of the LDL-cholesterol concentrations in midRamadan and end of Ramadan compared to concentration levels before Ramadan. Also, the results showed statistically significant elevation in the HDL-cholesterol concentrations in mid-Ramadan and end of Ramadan compared to levels before Ramadan. CONCLUSION The findings suggest the potential usefulness of Ramadan fasting in the restriction of fat intake which is associated with further lowering of serum cholesterol. These findings should influence future studies on hyperlipidemia.
Human D 3 dopamine receptor in the medulloblastoma TE 671 cell line : cross-talk between D 1 and D 3 receptors

In search of a cell line in which the D3 dopamine receptor is expressed endogenously, we found that the neuronderived human medulloblastoma cell line TE671 expresses the human D3 (hD3) and D1 (hD1) receptor, but neither the D2 or D4 receptors. Exposure of TE671 cells to the D3 agonist 7-OHDPAT (DPAT), or to the D1 agonist SKF-38393 (SKF) increased the expression of hD3 or hD1 mRNA, respectively. Moreover, whereas DPAT had no effect on hD1 mRNA levels, stimulating the cells with SKF caused an increase in both hD1 and hD3 transcript levels. These results suggest (i) that following ligand stimulation, hD3 and hD1 receptors are upregulated to enhance their own receptor expression, and (ii) that upregulation of hD1 receptor transcripts leads to a stimulation of the hD3 dopamine receptor transcripts. z 1998 Federation of European Biochemical Societies.
Numerical Analysis on the Multilayer Nanoring Waveguide Pair

We propose a multilayer nanoring waveguide pair and investigate the surface plasmon modes and gap field enhancement between the two multilayer nanoring waveguides. Due to the strongly coupled electric fields between multilayer nanoring waveguides, a field enhancement contrast ratio over 108 can be obtained. The mode refractive indices and the propagation lengths are analyzed by the commercial software COMSOL Multiphysics. We also demonstrate the influences of varied structure parameters on gap field enhancement. Moreover, graphene is replaced by gold in the multilayer nanoring waveguide pair to discuss the properties change. Thanks to the tunable surface conductivity, the gap field enhancement can be controlled by adjusting the chemical potentials of graphene. The application of multilayer nanoring waveguide pair may have a great prospect in photoelectric devices and bio-sensing.bio-sensing.
Direct and continuous measurement of dissolved carbon dioxide in freshwater aquatic systems—method and applications

Understanding of the processes that control CO2 concentrations in the aquatic environment has been hampered by the absence of a direct method to make continuous measurements over both short‐ and long‐term time intervals. We describe an in situ method in which a non‐dispersive infrared (NDIR) sensor is enclosed in a water impermeable, gas permeable polytetrafluoroethylene (PTFE) membrane and deployed in a freshwater environment. This allows measurements of CO2 concentration to be made directly at a specific depth in the water column without the need for pumps or reagents. We demonstrate the potential of the method using examples from different aquatic environments characterized by a range of CO2 concentrations (0·5–8·0 mg CO2‐C l−1, equivalent to ca 40–650 µmol CO2 l−1). These comprise streams and ponds from tropical, temperate and boreal regions. Data derived from the sensor was compared with direct measurements of CO2 concentrations using headspace analysis. Sensor performance following long‐term (>6 months) field deployment conformed to manufacturers' specifications, with no drift detected. We conclude that the sensor‐based method is a robust, accurate and responsive method, with a wide range of potential applications, particularly when combined with other in situ sensor‐based measurements of related variables. Copyright © 2009 John Wiley & Sons, Ltd.
Minimisation of divergence error in volumetric velocity measurements and implications for turbulence statistics

Volumetric velocity measurements performed in incompressible ﬂuids are typically hindered by a non zero divergence error due to experimental uncertainties. Here we present a technique to minimise divergence error by employing continuity of mass as a constraint, with minimal change to the measured velocity ﬁeld. The divergence correction scheme (DCS) is implemented using a constraint based non-linear optimisation. An assessment of DCS is performed using DNS velocity ﬁelds with random noise added to emulate experimental uncertainties, together with a Tomo-graphic PIV data set measured in a channel ﬂow facility at a matched Reynolds number to the DNS data ( Re (cid:28) ≈ 937). Results indicate that the divergence of the corrected velocity ﬁelds are reduced to near zero, and a clear improvement is evident in ﬂow statistics. In particular, signiﬁcant improvements are observed for statistics computed using spatial gradients such as the velocity gradient tensor, enstrophy and dissipation, where having zero divergence is most important.
Monochromatic Paths and Triangulated Graphs

This paper considers two properties of graphs, one geometrical and one topological, and shows that they are strongly related. Let G be a graph with four distinguished and distinct vertices, w1, w2, b1, b2. Consider the two properties, TRI+(G) and MONO(G), defined as follows. TRI+(G): There is a planar drawing of G such that all 3-cycles of G are faces; all faces of G are triangles except for the single face which is the 4-cycle (w1-b1-w2-b2-w1). MONO(G): G contains the 4-cycle (w1-b1-w2-b2-w1) and, for any labeling of the vertices of G by the colors {white, black} such that w1 and w2 are white, while b1 and b2 are black, precisely one of the following holds. There is a path of white vertices connecting w1 and w2. There is a path of black vertices connecting b1 and b2. Our main result is that a graph G enjoys property TRI+(G) if and only if it is minimal with respect to property MONO. Building on this, we show that one can decide in polynomial time whether or not a given graph G has property MONO(G).
Negative Binomial Model with an Application to Special Treatment Count Data

Special Treatment (ST) is a particular regulation for the exchange to prompt the risk to investors. Since ST count data are discrete, traditional Orthogonal Least Square method cannot get better goodness of fitting. However, Poisson regression is appropriate to be used to analyze discrete data. In addition, once it is over-dispersed, Negative Binomial (NB) regression model is more suitable. In this paper, we study the influence of non-financial factors on ST occurrence event count with NB model. Result shows that China Interbank Offered Rate, Import, Export, Deposits of Enterprises and one-period lagged ST count are evidently associated with ST risk.
Surface plasmons in graphene cylindrical waveguides

Surface plasmons (SPPs) supported by graphene cylindrical waveguides are investigated for the first time, and a rigorous dispersion relation is derived for the dominant mode. This relation reveals a fundamental connection between SPPs in carbon nanotubes and graphene sheets, which appear as limiting cases of plasmons in cylindrical waveguides. The proposed structure may find application in reconfigurable communication and sensing systems at THz.
Feature-Enriched Character-Level Convolutions for Text Regression

We present a new model for text regression that seamlessly combine engineered features and character-level information through deep parallel convolution stacks, multi-layer perceptrons and multi-task learning. We use these models to create the SHEF/CNN systems for the sentence-level Quality Estimation task of WMT 2017 and Emotion Intensity Analysis task of WASSA 2017. Our experiments reveal that combining character-level clues and engineered features offers noticeable performance improvements over us-ing only one of these sources of information in isolation.
Object-Tool-Actor Interaction: Object Information Drives Intended Action

ABSTRACT Tool use is typically explored via actor-tool interactions. However, the target-object (that which is being acted on) may influence perceived action possibilities and thereby guide action. Three different tool-target-object pairings were tested (Experiment 1). The hammering action demonstrated the greatest sensitivity and therefore subsequently used to further investigate target-object pairings. The hammer was removed as an option and instructions were provided using pictorial (Experiment 2), written (Experiment 3), and both pictorial and written formats (Experiment 4). The designed tool is chosen when available (Experiment 1) and when removed as a choice (i.e., the hammer), participants perform the same action associated with the designed tool (i.e., hammering) regardless of instruction method (Experiments 2, 3, and 4).
Development of a Biped Walking Robot with Antagonistic Actuation

This research was intended at developing a biped walking robot with antagonistic actuation for realizing stable walking motion. A three-linked planar-type walking robot was developed on the basis of the model of a semipassive walking robot. An antagonistic actuation mechanism based on the human musculoskeletal structure was applied to the hip joint of the developed walking robot. The antagonistic actuation was realized by using motors and wires. A control algorithm utilizing the antagonism for walking motion was proposed and applied to the walking experiment by using the developed robot. The results of the walking experiment conducted using the proposed control algorithm are presented and discussed.
Further stability analysis on Cohen–Grossberg neural networks with time-varying and distributed delays

The article is concerned with asymptotical stability for Cohen–Grossberg neural networks with both interval time-varying (0 ≤ τ0 ≤ τ(t) ≤ τ m ) and distributed delays, in which two types of distributed delays are treated: one is bounded while the other is unbounded. Through partitioning the delay intervals [0, τ0] and [τ0, τ m ], and choosing two augmented Lyapunov–Krasovskii functionals, some sufficient conditions are obtained to guarantee the global stability by employing the simplified free-weighting matrix method and convex combination. These stability criteria are presented in terms of linear matrix inequalities (LMIs) and can be easily checked by resorting to LMI in Matlab toolbox. Finally, three numerical examples are given to illustrate the effectiveness and reduced conservatism of the theoretical results.
Involvement of P2X4 and P2Y12 receptors in ATP‐induced microglial chemotaxis

We previously reported that extracellular ATP induces membrane ruffling and chemotaxis of microglia and suggested that their induction is mediated by the Gi/o‐protein coupled P2Y12 receptor (P2Y12R). Here we report discovering that the P2X4 receptor (P2X4R) is also involved in ATP‐induced microglial chemotaxis. To understand the intracellular signaling pathway downstream of P2Y12R that underlies microglial chemotaxis, we examined the effect of two phosphatidylinositol 3′‐kinase (PI3K) inhibitors, wortmannin, and LY294002, on chemotaxis in a Dunn chemotaxis chamber. The PI3K inhibitors significantly suppressed chemotaxis without affecting ATP‐induced membrane ruffling. ATP stimulation increased Akt phosphorylation in the microglia, and the increase was reduced by the PI3K inhibitors and a P2Y12R antagonist. These results indicate that P2Y12R‐mediated activation of the PI3K pathway is required for microglial chemotaxis in response to ATP. We also found that the Akt phosphorylation was reduced when extracellular calcium was chelated, suggesting that ionotropic P2X receptors are involved in microglial chemotaxis by affecting the PI3K pathway. We therefore tested the effect of various P2X4R antagonists on the chemotaxis, and the results showed that pharmacological blockade of P2X4R significantly inhibited it. Knockdown of the P2X4 receptor in microglia by RNA interference through the lentivirus vector system also suppressed the microglial chemotaxis. These results indicate that P2X4R as well as P2Y12R is involved in ATP‐induced microglial chemotaxis. © 2007 Wiley‐Liss, Inc.
Knowledge pruning in decision trees

We propose a novel pruning method of decision trees based on domain knowledge, semantic hierarchies among classes, which is used to generate decision trees by relaxing the levels of hierarchies for both height and width of the trees. We develop the algorithm, and the effectiveness is examined by UCI Machine Learning Repository: On Car Evaluation and Nursery. We can generate the decision trees consisting of 11 and 13 rules, although C4.5 generates 182 and 572 rules, respectively.
Inflectional frames in language production

The authors report six implicit priming experiments that examined the production of inflected forms. Participants produced words out of small sets in response to prompts. The words differed in form or shared word-initial segments, which allowed for preparation. In constant inflectional sets, the words had the same number of inflectional suffixes, whereas in variable sets the number of suffixes differed. In the experiments, preparation effects were obtained, which were larger in the constant than in the variable sets. Control experiments showed that this difference in effect was not due to syntactic class or phonological form per se. The results are interpreted in terms of a slot-and-filler model of word production, in which inflectional frames, on the one hand, and stems and affixes, on the other hand, are independently spelled out on the basis of an abstract morpho-syntactic specification of the word, which is followed by morpheme-to-frame association.
Equalization of EV and HEV batteries with a ramp converter

Valve regulated lead acid (VRLA) batteries used on EVs and HEVs are subject to voltage imbalances which greatly diminish battery lifetime and vehicle range. An equalizer that is implemented with a high frequency, soft switching converter that corrects this imbalance is described here. Experimental results are presented for a pack of 27 batteries.
Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes

ABSTRACT A new fluorescence in situ hybridization (FISH) method with peptide nucleic acid (PNA) probes for identification of Staphylococcus aureus directly from positive blood culture bottles that contain gram-positive cocci in clusters (GPCC) is described. The test (the S. aureus PNA FISH assay) is based on a fluorescein-labeled PNA probe that targets a species-specific sequence of the 16S rRNA of S. aureus. Evaluations with 17 reference strains and 48 clinical isolates, including methicillin-resistant and methicillin-susceptible S. aureus species, coagulase-negative Staphylococcus species, and other clinically relevant and phylogenetically related bacteria and yeast species, showed that the assay had 100% sensitivity and 96% specificity. Clinical trials with 87 blood cultures positive for GPCC correctly identified 36 of 37 (97%) of the S. aureus-positive cultures identified by standard microbiological methods. The positive and negative predictive values were 100 and 98%, respectively. It is concluded that this rapid method (2.5 h) for identification of S. aureus directly from blood culture bottles that contain GPCC offers important information for optimal antibiotic therapy.