Miracle-dz/UniFAR-FTUs huggingface dataset:
Attenuation of DNA damage‐induced p53 expression by arsenic: A possible mechanism for arsenic co‐carcinogenesis

Inhibition of DNA repair processes has been suggested as one predominant mechanism in arsenic co‐genotoxicity. However, the underlying mode of action responsible for DNA repair inhibition by arsenic remains elusive. To further elucidate the mechanism of repair inhibition by arsenic, we examined the effect of trivalent inorganic and methylated arsenic metabolites on the repair of benzo(a)pyrene diol epoxide (BPDE)–DNA adducts in normal human primary fibroblasts and their effect on repair‐related protein expression. We observed that monomethylarsonous acid (MMAIII) was the most potent inhibitor of the DNA repair. MMAIII did not change the expression levels of some key repair proteins involved upstream of the dual incision in the global nucleotide excision repair (NER) pathway, including p48, XPC, xeroderma pigmentosum complementation group A (XPA), and p62‐TFIIH. However, it led to a marked impairment of p53 induction in response to BPDE treatment. The abrogated p53 expression translated into reduced p53 DNA‐binding activity, suggesting a possibility of downregulating downstream repair genes by p53. A p53‐null cell line failed to exhibit the inhibitory effect of MMAIII on NER, implicating a role for p53 in the NER inhibition by MMAIII. Further investigation revealed that MMAIII dramatically inhibited p53 phosphorylation at serine 15, implying that MMAIII destabilized p53 by inhibiting its phosphorylation. Because p53 is required for proficient global NER, our data suggest that arsenic inhibits NER through suppressing p53 induction in response to DNA damage in cells with normal p53 gene expression. © 2007 Wiley‐Liss, Inc.
On adaptive gain tuning for wireless Ad Hoc networks

We have proposed an adaptive gain scheme for resource allocation in wireless ad hoc networks based on single neural node and virtual price concept. The degree of congestion on a wireless link can be symbolized an aggregate virtual price along a route, which depends mostly on the wireless nodepsilas queue occupancy. This approach adopts an adaptive approach to control the transmission rate at the source, named ASR controller. Our object is to optimize the resource allocation dynamically through the transmission rate adjustment and stabilized the queue size of wireless links. The basic control theory approach for the algorithm is been presented. The performance of simulation indicates that the proposed scheme can achieve the goal of optimal channel capacity sharing among multi-hop flows and sharing bandwidth fairly.
“I Am Not A Victim. I Am A Survivor”: Resilience as a Journey for Female Survivors of Child Sexual Abuse

ABSTRACT This study was designed to expand our understanding of the positive aspects of coping and resilience in female survivors of child sexual abuse. Research questions focused on women’s lived experiences of being survivors of child sexual abuse and how they have experienced resilience, developed healthy intimate relationships, and viewed themselves as sexual beings. Using a qualitative research lens of phenomenology, we captured the essence of survivors’ experiences of resilience. Although each woman’s experience was unique, similar patterns of processes and outcomes emerged as meaningful in their development of resilient and healthy sexuality and relationship functioning. However, participants emphasized resilience as a process or journey—recovery from trauma, reconceptualization of self, and development of healthy sexuality included deliberate efforts occurring over time. Implications for future research and practice using a positive lens of resilience are provided.
Molecular survey of RNA viruses in Hungarian bats: discovering novel astroviruses, coronaviruses, and caliciviruses.

UNLABELLED Background: Bat-borne viruses pose a potential risk to human health and are the focus of increasing scientific interest. To start gaining information about bat-transmitted viruses in Hungary, we tested multiple bat species for several virus groups between 2012 and 2013. MATERIALS AND METHODS Fecal samples were collected from bats across Hungary. We performed group-specific RT-PCR screening for astro-, calici-, corona-, lyssa-, othoreo-, paramyxo-, and rotaviruses. Positive samples were selected and sequenced for further phylogenetic analyses. RESULTS A total of 447 fecal samples, representing 24 European bat species were tested. Novel strains of astroviruses, coronaviruses, and caliciviruses were detected and analyzed phylogenetically. Out of the 447 tested samples, 40 (9%) bats were positive for at least one virus. Bat-transmitted astroviruses (BtAstV) were detected in eight species with a 6.93% detection rate (95% confidence interval [CI] 4.854, 9.571). Coronaviruses (BtCoV) were detected in seven bat species with a detection rate of 1.79% (95% CI 0.849, 3.348), whereas novel caliciviruses (BtCalV) were detected in three bat species with a detection rate of 0.67% (95% CI 0.189, 1.780). Phylogenetic analyses revealed a great diversity among astrovirus strains, whereas the Hungarian BtCoV strains clustered together with both alpha- and betacoronavirus strains from other European countries. One of the most intriguing findings of our investigation is the discovery of novel BtCalVs in Europe. The Hungarian BtCalV did not cluster with any of the calcivirus genera identified in the family so far. CONCLUSIONS We have successfully confirmed BtCoVs in numerous bat species. Furthermore, we have described new bat species harboring BtAstVs in Europe and found new species of CalVs. Further long-term investigations involving more species are needed in the Central European region for a better understanding on the host specificity, seasonality, phylogenetic relationships, and the possible zoonotic potential of these newly described viruses.
Innovative Applications of O . R . The Latency Location-Routing Problem

This paper introduces the Latency Location-Routing Problem (LLRP) whose goal is to minimize waiting time of recipients by optimally determining both the locations of depots and the routes of vehicles. The LLRP is customer oriented by pursuing minimization of the latency instead of minimization of the length of routes. One of the main applications of this problem is the distribution of supplies to affected areas in post-disaster relief activities. It is also relevant in customer-oriented supply chain where latency at demand locations plays a significant role in the satisfaction of the customers. The problem is formulated mathematically and two heuristics, the Memetic Algorithm (MA) and the Recursive Granular Algorithm (RGA), are proposed. An extensive experimental study shows that both algorithms are able to produce promising results in reasonable time. © 2016 Elsevier B.V. All rights reserved.
Maximally Flat-Frequency-Response Multiple-Resonator-Based Harmonic Analysis

This paper presents an improved approach to the recently proposed multiple-resonator-based method for the harmonic analysis that has been provided in the previous papers. Previously, two inherent particular cases have been considered. In these cases, reference points in which estimation is performed are located either in the middle or at the end of the observation interval. The first case exhibits a good noises and unwanted harmonics attenuation but possesses a large delay time. In the second case, the filters are able to form a zero-flat phase response about the operation frequency and hence able to provide instantaneous estimates, but with large overshoots caused by resonant frequencies at the edges of the passband, and the high level of the sidelobes, that also makes it susceptible to interharmonics and noise interference. The aim of this paper is to propose a compromised solution provided by the tradeoff between those opposite requirements by shifting this point along the observation interval. This way the frequency responses of the estimator are reshaped. A maximally flatness of the frequency response in the operation harmonic frequency is kept in all cases, but only locating the reference point in a fraction around the center of the observation interval provides flat-top frequency responses. The effectiveness of the proposed estimation technique is shown through simulations.
Algoritmo para entrega de alarmes em redes de sensores sem fio utilizando veículos aéreos não tripulados

Este trabalho apresenta a investigacao de uma estrategia para coordenar um conjunto de nos sensores terrestres estaticos (posicionados no solo) e de Veiculos Aereos Nao Tripulados (VANTs) que carregam uma variedade de sensores. Essa coordenacao tem como objetivo prover monitoramento e deteccao eficientes de intrusos em uma determinada area de interesse. Para o desenvolvimento desta coordenacao tem-se o uso de tecnicas de Auto-Organizacao Emergente. Essas tecnicas nao utilizam controles externos ou centralizados, contudo, e gerado um comportamento global emergente a partir das pequenas e simples interacoes locais entre os individuos do sistema. Os nos sensores terrestres sao configurados para acionar alarmes na ocorrencia de entrada de um intruso na area, enquanto os UAVs recebem os alarmes e tem que decidir qual UAV e o mais habil a tratar o alarme acionado. Ao fim, os resultados demonstram a viabilidade da estrategia utilizada em relacao ao overhead de mensagens enviadas
Automatic delineation of single-lead electrocardiograph fiducial points based on the hierarchical triple-extreme-points model

This paper introduces a novel single-lead electrocardiograph (ECG) automatic delineator based on the hierarchical triple-extreme-points model (HTM) and self-learning, featuring high robustness, low computational cost and mathematical simplicity. By applying HTM to obtaining the local morphological features of ECG signals, this method is capable of precisely detecting QRS complexes, P-wave and T-wave. In the experimental studies, this method was validated by several standard real-world ECG databases, including MIT-BIH arrhythmia, QT, European ST-T and TWA Challenge 2008 databases. For QRS detection, the average sensitivity value was 99.74% and the positive predictivity value was 99.80% for all databases. In the meantime, for P-wave and T-wave detection on QT, the sensitivity values were 99.49% and 99.81% respectively and the positive predictivity values were 98.82 and 99.80% respectively. As to delineation, the average maximum delineation error was no more than 4 ms and the standard deviation error was around 10ms for P-wave, QRS complex and T-wave.
Load-Based Route Discovery Through Searching Range Adaptation for MANET Throughput Improvement

Traditional routing protocols designed for multihop wireless networks, e.g., the mobile ad hoc network (MANET), select a route with the smallest hop count. The end-to-end throughput in the route may not be the maximum when the network has multiple link data rates, as a small hop number implies a large geographic distance (link distance) for each hop. Due to the radio signal attenuation, this results in a low SNR at the receiving node and, consequently, a low available link data rate. Reducing the link distance by using routes with more hop counts can increase the link data rate. However, this may not necessarily improve the end-to-end throughput because more nodes have to be included in the routes. In this paper, we first investigate and analyze the impact of link distance on end-to-end throughput in multirate multihop wireless networks. Analysis and simulation results show that changing the link distance affects the network throughput. To achieve a high network throughput, a proper link distance requirement has to be set for each hop, depending on different parameters such as load density. Based on this finding, we then propose a crossing-layer adaptive searching range (ASR) routing algorithm. ASR can be integrated into existing routing protocols. According to the local network load density, ASR can help improve the network performance by adjusting the link distance in the routes.
The GLP-1 receptor agonist liraglutide inhibits progression of vascular disease via effects on atherogenesis, plaque stability and endothelial function in an ApoE−/− mouse model

Liraglutide, a once-daily glucagon-like peptide-1 receptor (GLP-1R) agonist, has been approved as a new treatment for type 2 diabetes and is the subject of a clinical trial programme to evaluate the effects on cardiovascular disease and safety. The current study aimed to determine the in vivo effect of liraglutide on progression of atherosclerotic vascular disease in the apolipoprotein E-deficient (ApoE−/−) mouse model and identify underlying mechanisms responsible. Liraglutide treatment inhibited progression of early onset, low-burden atherosclerotic disease in a partially GLP-1R-dependent manner in the ApoE−/− mouse model. In addition, liraglutide treatment inhibited progression of atherosclerotic plaque formation and enhanced plaque stability, again in a partially GLP-1R-dependent manner. No significant effect of liraglutide on progression of late onset, high-burden atherosclerotic disease was observed. In addition, no significant endothelial cell dysfunction was identified in ApoE−/− mice with early onset, low-burden atherosclerotic disease, although significant prevention of weight gain was observed in liraglutide-treated mice using this dietary protocol. Taken together, these results suggest a potential role for liraglutide in the prevention and stabilisation of atherosclerotic vascular disease together with possible protection against major cardiovascular events.
Reference guide to the stages of chick heart embryology

Cardiac progenitors of the splanchnic mesoderm (primary and secondary heart field), cardiac neural crest, and the proepicardium are the major embryonic contributors to chick heart development. Their contribution to cardiac development occurs with precise timing and regulation during such processes as primary heart tube fusion, cardiac looping and accretion, cardiac septation, and the development of the coronary vasculature. Heart development is even more complex if one follows the development of the cardiac innervation, cardiac pacemaking and conduction system, endocardial cushions, valves, and even the importance of apoptosis for proper cardiac formation. This review is meant to provide a reference guide (Table 1 ) on the developmental timing according to the staging of Hamburger and Hamilton ( 1951 ) (HH) of these important topics in heart development for those individuals new to a chick heart research laboratory. Even individuals outside of the heart field, who are working on a gene that is also expressed in the heart, will gain information on what to look for during chick heart development. This reference guide provides complete and easy reference to the stages involved in heart development, as well as a global perspective of how these cardiac developmental events overlap temporally and spatially, making it a good bench top companion to the many recently written in‐depth cardiac reviews of the molecular aspects of cardiac development. Developmental Dynamics 233:1217–1237, 2005. © 2005 Wiley‐Liss, Inc.
The Relationship between Non-Suicidal Self-Injury and the UPPS-P Impulsivity Facets in Eating Disorders and Healthy Controls

In the present study, we investigated the association between Non-Suicidal Self-Injury (NSSI) and the UPPS-P impulsivity facets in eating disorder patients and healthy controls. The prevalence of NSSI in eating disorder (ED) patients ranged from 17% in restrictive anorexia nervosa (AN-R) patients to 43% in patients with bulimia nervosa (BN). In healthy controls (HC), the prevalence of NSSI was 19%. Eating disorder patients from the binge eating/purging type showed significantly more NSSI compared to restrictive ED and HC participants. Binge-eating/purging ED patients also scored significantly higher on Negative/Positive Urgency, Lack of Premeditation and Lack of Perseverance compared to HC and restrictive ED patients. Comparable findings were found between ED patients and HC with and without NSSI; ED patients and HC with NSSI scored significantly higher in four of the five UPPS-P dimensions compared to participants without NSSI; Sensation Seeking was the exception. Finally, the presence of NSSI in HC/ED patients was particularly predicted by low levels of Perseverance. Therefore, the treatment of ED patients with NSSI certainly needs to focus on the training of effortful control.
Kinetics and steady‐state properties of the charged system controlling sodium conductance in the squid giant axon

1. Asymmetries in the early time course of the displacement current passing across the membrane after application of equal voltage‐clamp pulses in the two directions have been investigated in the squid giant axon. Before making the measurements, Na current was blocked by removal of external Na and treatment with tetrodotoxin. Potassium current was usually blocked by perfusion with CsF, but some experiments were done with intact axons. A signal averaging technique was used to eliminate the symmetrical components of the membrane current.
Least squares identification method for differential equations of gene regulatory networks

This paper transforms a differential equation model of a gene regulatory network into a discrete equation by using the explicit Euler method. A recursive least squares algorithm is presented to generate the parameter estimates by replacing the unknown true outputs with their estimates. The simulation results indicate that the proposed algorithm is effective and is of a high estimation accuracy.
Ultrabroadband Optical Phenomena in Quadratic Nonlinear Media

We present a comprehensive framework to study the nonlinear evolution of ultrabroadband optical pulses in quadratic nonlinear media. We employ a nonlinear envelope equation that goes beyond the traditional slowly varying approximation and allows treatment of all the harmonics by means of a single equation. We exploit this model to simulate recently observed supercontinuum phenomena such as ultrabroadband parametric downconversion and the generation of octave-spanning spectra from femtosecond pulses.
Depth-of-Field Extension Method Using Variable Annular Pupil Division

The depth-of-field (DOF) of an imaging system is closely related to the pupil function that describes the characteristics of the pupil such as size and shape. Shallow DOF is especially problematic in microscopic optical imaging systems because they have relatively large aperture size for high optical resolution. In this paper, a new DOF extension method is proposed. This is to acquire an image by integrating the incoming light while changing the size of annular pupil. By dividing the pupil into annular form, the effect of the defocus aberration is reduced. Theoretical background is explained and simulation and experimental results are shown for verification. The results show that the DOF can effectively be extended by this method.
Ephrin-B1 and ephrin-B2 mediate EphB-dependent presynaptic development via syntenin-1

The development of central nervous system synapses requires precise coordination between presynaptic and postsynaptic components. The EphB family controls postsynaptic development by interacting with glutamate receptors and regulating dendritic filopodia motility, but how EphBs induce the formation of presynaptic specializations is less well understood. Here, we show that knockdown of presynaptic ephrin-B1, ephrin-B2, or syntenin-1, but not ephrin-B3, prevents EphB-dependent presynaptic development. Ephrin-B1, ephrin-B2, and syntenin-1 are clustered together with presynaptic markers, suggesting that these molecules function jointly in presynaptic development. Knockdown of ephrin-B1 or ephrin-B2 reduces the number of synaptic specializations and the colocalization of syntenin-1 with synaptic markers. Simultaneous knockdown of ephrin-B1 and ephrin-B2 suggests that they function independently in the formation of synaptic contacts, but act together to recruit syntenin-1 to presynaptic terminals. Taken together, these results demonstrate that ephrin-B1 and ephrin-B2 function with EphB to mediate presynaptic development via syntenin-1.
High Frequency Regeneration in Important Medicinal Plant Uraria picta Jacq. DC

Present investigation is aimed to develop the high frequency regeneration in Uraria picta. This plant is overexploited for preparation of the drug Dashmula. Explants used during investigation were axillary buds, segment of leaves and nodal segments which responded well to all growth regulators. High frequency regeneration of shoots was achieved within 21 days on modified Murashige and Skoog's medium supplemented with 0.5 mg/L of Indole-3-acetic acid along with 2.5 mg/L 6-benzylaminopurine. Isolated shoots rooted on MS along with in 0.5 mg/L indole-3-acetic acid. In vitro raised plants after hardening were grew normally in soil after treatment with inorganic salt solution.
Adenovirus type 2 VAI RNA transcription by polymerase III is blocked by sequence-specific methylation

Sequence-specific methylation of the promoter and adjacent regions in mammalian genes transcribed by RNA polymerase II leads to the inhibition of these genes. So far, RNA polymerase III-transcribed genes have not been investigated in depth. We therefore studied methylation effects on the RNA polymerase III-transcribed VAI gene of adenovirus type 2 DNA. The VAI gene contains 20 5'-CG-3' dinucleotides, of which 4 (20%) can be methylated by HpaII (5'-CCGG-3') and HhaI (5'-GCGC-3'). Three of these 5'-CG-3' sequences are located close to the internal regulatory region of the VAI segment. An unmethylated, a 5'-CCGG-3'- and 5'-GCGC-3'-methylated, and a 5'-CG-3'-methylated pUC18 construct containing the VAI and VAII regions were transfected into mammalian cells. In many experiments, an inactivating effect of 5'-CCGG-3' and 5'-GCGC-3' DNA methylation on the VAI region was not observed. In contrast, methylation of all 20 5'-CG-3' sequences in the VAI region by a CpG-specific DNA methyltransferase from Spiroplasma species did interfere with VAI transcription. Transcription of the VAI- and VAII- and of the VAI-containing constructs was also shown to be inhibited in an in vitro cell-free transcription system after the constructs had been methylated at the 5'-CCGG-3' and 5'-GCGC-3' sequences or at all 5'-CG-3' sequences. When an oligodeoxyribonucleotide which carried the internal control block A of the VAI region was methylated at three 5'-CG-3' sequences, the formation of a complex with HeLa nuclear proteins was abrogated. The results presented support the notion that the VAI gene transcribed by the DNA-dependent RNA polymerase III is also inactivated by methylation of the decisive 5'-CG-3' sequences.
The Th228 decay series in adult beagles: Ra224, Pb212, and Bi-212 in blood and excreta.

The studies to be reported are a part of the long-term studies of the biological effects of Th228 and other radionuclides in adult beagles (1). Since the Th228 decay products, Ra224, Tn220, Pb212, and Bi212, are not completely retained at the sites at which they are formed in the dog, extensive studies of their retention and distribution are needed if estimates of radiation dose rates are to be made. Previously the metabolism in these beagles of the parent nuclide, Th228, over a period of 3.5 years after injection has been described (2). Earlier, Van Dilla et al. (3) reported the translocation of Ra224 during the first few months after injection, and Mays et al. (4) reported the exhalation of Tn220 through 1000 days. This paper gives the results of the measurements of the concentration in blood plasma and the urinary and fecal excretion of Ra224 (3.64-day half-period), and the concentration in blood cells of Pb212 (10.6-hour half-period), through 3.5 years. Also presented are less extensive studies of urinary excretion of Pb212, and urinary excretion and plasma and blood cell concentrations of Bi212 (60.5-minute half-period). From these results estimates of the retentions of these decay products are made.