Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression pattern of a ‘Plus‐C’ class odorant binding protein in the antenna of the malaria vector Anopheles gambiae

In the malaria mosquito Anopheles gambiae (Ag), olfaction plays a crucial role in various behaviours, most strikingly in the seeking of females after a blood meal. The first step of odorant recognition in antennal sensilla involves soluble odorant binding proteins (OBPs), which transfer odorous compounds to olfactory receptors (ORs) in the dendritic membrane of sensory neurons. A particular OBP subtype of the ‘Plus‐C’ class, called AgOBP48, is abundantly transcribed in female antennae and partially down‐regulated after a blood meal, suggesting a possible role in host detection. In the present study, we have identified the AgOBP48‐expressing cells, explored their antennal topography and determined their position relative to cells that express the ‘classic’ AgOBP1, the AgOR co‐receptor (AgOrco) and the receptor AgOR1. By means of two‐colour whole‐mount fluorescence in situ hybridization it was found that AgOBP48 was expressed in cells, which are closely associated with AgOrco‐expressing sensory neurons. Furthermore, AgOBP48 was not expressed in the same cells as AgOBP1, but subpopulations of AgOBP48‐ and of AgOBP1‐expressing cells were found closely associated and adjacent to sensory neurons expressing AgOR1. Together, the results indicate that cells that express either AgOBP48, AgOBP1 or AgOR1 are housed together in distinct olfactory sensilla and that an interplay of the proteins may contribute to the specific responsiveness of the sensillum to distinct odorants.
Cost-effectiveness of directly observed chemoprophylaxis of tuberculosis among drug users at high risk for tuberculosis.

SETTING A methadone treatment program with on-site medical care in the Bronx, New York. OBJECTIVE To define whether costs associated with directly observed preventive therapy (DOPT) of tuberculosis are justified by cases and costs of tuberculosis prevented among persons at high risk for active disease. DESIGN Detailed data were collected on drug users in treatment regarding human immunodeficiency virus (HIV) and tuberculosis infection and disease, and costs of screening, chemoprophylaxis, direct observation and treatment of active disease. The cost-effectiveness of providing DOPT to this population was modeled. RESULTS We assessed the impact of providing DOPT to 151 eligible persons. Assuming 65% isoniazid effectiveness, and incorporating costs of screening, observed chemoprophylaxis and clinical monitoring, a net savings in tuberculosis-related hospital costs of $285,284 ($563 per person screened) was associated with DOPT ($10,274 per case prevented). Direct observation of chemoprophylaxis proved cost-effective if associated with even a 10% increment in overall isoniazid effectiveness compared with self-administered chemoprophylaxis. DOPT costs per tuberculosis case averted remained below the in-patient costs of a single case of drug-sensitive disease across a range of parameter values. CONCLUSIONS Providing DOPT is a highly cost-effective intervention for drug users in treatment. Commitment of additional resources required for DOPT should be given priority in this and other populations at high risk for tuberculosis.
A Very Uneven Playing Field: Economic Mobility in the United States

We present results from a new data set, the Statistics of Income Mobility Panel, that has been assembled from tax and other administrative sources to provide evidence on economic mobility and persistence in the United States. This data set allows us to take on the methodological problems that have complicated previous efforts to estimate intergenerational earnings and income elasticities. We find that the elasticities for women’s income, men’s income, and men’s earnings are as high as all but the highest of the previously reported survey-based estimates. Because the intergenerational curves are especially steep within the parental-income region defined by the 50th to 90th percentiles, approximately two-thirds of the inequality between poor and well-off families is passed on to the next generation. This extreme persistence cannot be attributed to any single factor. Instead, the U.S. is exceptional with respect to virtually all factors governing economic persistence, including the returns to human capital, the amount of public investment in the human capital of low-income children, the amount of socioeconomic segregation, and the progressiveness of the tax-and-transfer system. For each of these four factors, the U.S. has opted for policies that are mobility-reducing, with the implication that any substantial increase in mobility will likely require a wide-ranging package of reforms that cut across many institutions.
Crowdfunding and Social Entrepreneurship: Spotlight on Intermediaries

This study contributes to the literature by describing how crowdfunding platforms that host social entrepreneurship projects build and preserve legitimacy. We study three intermediaries, analyzing the actions they take to ensure that creators and funders perceive crowdfunding as a trustworthy form of alternative finance. This study shows that the legitimacy that funders ascribe to a project’s social and/or environmental aims is also a source of legitimacy for the intermediaries that promote social entrepreneurship projects. These intermediaries act as agents of social change, using a range of mechanisms to promote projects that seek to create social and/or environmental value in addition to economic value. Our study also has practical implications. We highlight the mechanisms used to reduce potential risks for intermediaries, creators, and funders and ensure their trust in crowdfunding.
Bioinspired nanofibers support chondrogenesis for articular cartilage repair

Articular cartilage repair remains a significant and growing clinical challenge with the aging population. The native extracellular matrix (ECM) of articular cartilage is a 3D structure composed of proteinaceous fibers and a hydrogel ground substance that together provide the physical and biological cues to instruct cell behavior. Here we present fibrous scaffolds composed of poly(vinyl alcohol) and the biological cue chondroitin sulfate with fiber dimensions on the nanoscale for application to articular cartilage repair. The unique, low-density nature of the described nanofiber scaffolds allows for immediate cell infiltration for optimal tissue repair. The capacity for the scaffolds to facilitate cartilage-like tissue formation was evaluated in vitro. Compared with pellet cultures, the nanofiber scaffolds enhance chondrogenic differentiation of mesenchymal stems cells as indicated by increased ECM production and cartilage specific gene expression while also permitting cell proliferation. When implanted into rat osteochondral defects, acellular nanofiber scaffolds supported enhanced chondrogenesis marked by proteoglycan production minimally apparent in defects left empty. Furthermore, inclusion of chondroitin sulfate into the fibers enhanced cartilage-specific type II collagen synthesis in vitro and in vivo. By mimicking physical and biological cues of native ECM, the nanofiber scaffolds enhanced cartilaginous tissue formation, suggesting their potential utility for articular cartilage repair.
Specific immune responses restored by alteration in carbohydrate chains of surface molecules on antigen‐presenting cells

Two class I major histocompatibility (MHC) mutant mouse strains, H‐2bm14 and H‐2bm6, differ from the strain of origin C57BL/6 (B6, H‐2b) in one and two amino acids of the H‐2Db and H‐2Kb molecule, respectively. The bm14 Db mutation results in specific failure of female bm14 mice to generate a cytotoxic T lymphocyte (Tc) response to the male‐specific antigen H‐Y. The allospecific Tc response of CD8 B6 T cells against bm6 Kb mutant spleen cells, in contrast to that against other Kb mutants, is absolutely CD4 T helper cell dependent. Purified CD8 T cells completely fail to respond. We now report that the inability to mount these specific immune responses is restored by the use of dendritic cells (DC) as antigen‐presenting cells (APC). Comparison of MHC expression on various types of APC by cytofluorimetry and quantitative immunoprecipitation showed very high expression of class I and class II MHC molecules on DC. Strikingly, examination of class I and class II molecules by isoelectric focusing revealed qualitative differences as well. We show that the surface MHC class I molecules of DC are present in greater quantity and carry on average fewer sialic acids than the same molecules isolated from other APC types such as spleen cells, lipopolysaccharide blasts or concanavalin A blasts. That sialic acids on cell surface molecules, including MHC, may play a role in antigen presentation is suggested by our finding that removal of sialic acids, by neuraminidase, can restore specific responses to nonresponder APC as well.
Perceived fatigue interference and depressed mood: comparison of chronic fatigue syndrome/myalgic encephalomyelitis patients with fatigued breast cancer survivors

Objective: Persistent fatigue and depressive symptoms are both highly prevalent among patients with chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) as well as breast cancer survivors. This study aimed to assess and directly compare perceptions of fatigue as highly interfering in one's daily functioning in both patient populations to better understand their relationships with depressed mood. Methods: Participants were 95 female CFS/ME patients and 67 females who were approximately five years post-treatment for stage 0–III breast cancer presenting with clinically elevated fatigue severity. Self-report measures were obtained on participants' fatigue-related interference in daily functioning and fatigue severity as well as depressed mood. Hierarchical regression was used to test effects controlling for relevant demographic, psychosocial, and medical covariates. Results: CFS/ME patients endorsed greater depressed mood and fatigue interference than did fatigued breast cancer survivors, p < . 001. These factors were significantly positively correlated among CFS/ME patients (β = .36, p < .001), but not among the fatigued breast cancer survivors (β = .18, p = .19). Conclusions: CFS/ME patients reported elevated fatigue symptoms and depression relative to fatigued breast cancer survivors. In the former group, greater depressed mood was highly and significantly associated with greater fatigue-related inference in daily activities. Potential targets for cognitive behavioral interventions are discussed.
Plasma homocysteine level and its relationship to clinical profile in Parkinson's disease patients at the Lagos University Teaching Hospital.

BACKGROUND Hyperhomocysteinaemia (HHcy) is as a long-term sequelum of levodopa therapy in Parkinson's disease (PD). Information on its frequency and effects in Africans with PD is sparse. OBJECTIVE To determine the frequency of HHcy and its relationship to clinical features of PD in African patients. METHODS Using a case-control design, 40 consecutively attending PD patients and 40 age- and gender-matched healthy volunteering controls were studied. Parkinson's disease cases were evaluated for disease and treatment characteristics, using the Unified Parkinson Disease Rating Scale (UPDRS) motor and activities of daily living scores and disease stage (Hoehn and Yahr scale). Fasting total plasma homocysteine (Hcy) was determined in all subjects. Hyperhomocysteinaemia was defined as a Hcy level above the 90th percentile for the controls. RESULTS Mean Hcy was 13.8 ± 5.4mmol/L in PD and 12.4±3mmol/L in controls (P>0.05). Hyperhomocysteinaemia (Hcy > 16.26umol/L) occurred in nine (22.5%) PD patients (all on levodopa) and 6 (15%) controls (P>0.05). Mean duration of levodopa use was 92 ± 105.3 months in PD with HHcy compared to PD patients with normal Hcy 33.9 ± 33.2 (p < 0.05). Disease severity and disability were similar regardless of Hcy levels. None of current age, disease duration, Hoehn and Yahr stage, UPDRS scores, total levodopa dose and duration was independent predictor of homocysteine level. CONCLUSION There is increased occurrence of hyper-homocysteinaemia in Nigerian subjects with Parkinson's disease, receiving Levodopa. This hyperhomocysteinaemia is more common with prolonged use but appears to have no relationship with disease severity or disability.
Steep anomalous dispersion in coherently prepared Rb vapor

Steep dispersion of opposite signs in driven degenerate two-level atomic transitions have been predicted and observed on the ${D}_{2}$ line of ${}^{87}\mathrm{Rb}$ in an optically thin vapor cell. The intensity dependence of the anomalous dispersion has been studied. The maximum observed value of anomalous dispersion ( $\mathrm{dn}/d\ensuremath{\nu}\ensuremath{\simeq}\ensuremath{-}6\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}11}{\mathrm{Hz}}^{\ensuremath{-}1}$) corresponds to a negative group velocity ${V}_{g}\ensuremath{\simeq}\ensuremath{-}c/23000$.
MicroRNA expression during chick embryo development

MicroRNAs (miRNAs) are small, abundant, noncoding RNAs that modulate protein abundance by interfering with target mRNA translation or stability. miRNAs are detected in organisms from all domains and may regulate 30% of transcripts in vertebrates. Understanding miRNA function requires a detailed determination of expression, yet this has not been reported in an amniote species. High‐throughput whole mount in situ hybridization was performed on chicken embryos to map expression of 135 miRNA genes including five miRNAs that had not been previously reported in chicken. Eighty‐four miRNAs were detected before day 5 of embryogenesis, and 75 miRNAs showed differential expression. Whereas few miRNAs were expressed during formation of the primary germ layers, the number of miRNAs detected increased rapidly during organogenesis. Patterns highlighted cell‐type, organ or structure‐specific expression, localization within germ layers and their derivatives, and expression in multiple cell and tissue types and within sub‐regions of structures and tissues. A novel group of miRNAs was highly expressed in most tissues but much reduced in one or a few organs, including the heart. This study presents the first comprehensive overview of miRNA expression in an amniote organism and provides an important foundation for investigations of miRNA gene regulation and function. Developmental Dynamics 235:3156–3165, 2006. © 2006 Wiley‐Liss, Inc.
Fabrication of Nanopillars based on Silicon Oxide Nanopatterns Synthesized in Oxygen Plasma Removal of Photoresist

We report for the first time a facile lithography-free approach for fabricating nanopillars over large areas or in patterns. The key technique of this approach is that randomly-distributed nanoscale SiO2 patterns can be synthesized on substrates simply by removing photoresist with oxygen plasma bombardment. Those SiO2 nanopatterns may further function as masks in the following etching process for nanopillars. Based on this approach, a variety of microstructures containing nanopillars with diameters of 30~200 nm, which include surface micro channels, micro-cantilever probes and nanofences, have been fabricated. This approach can be applied both to silicon and metal substrates compatible with conventional micro-electromechanical systems (MEMS) fabrication.
Extracellular Matrix and Matrix Metalloproteinase Changes in Human Corneas After Complicated Laser-Assisted In Situ Keratomileusis (LASIK)

Purpose. To characterize extracellular matrix (ECM) and nine matrix metalloproteinase (MMP) changes in two corneas that underwent a complicated laser-assisted in situ keratomileusis (LASIK) procedure. Methods. The first patient underwent bilateral LASIK. The flap on the left eye was transected in several locations and placed back. This cornea later developed edema, and the removed flap was analyzed after lamellar keratoplasty. The second patient had a LASIK flap lifted, replaced twice, and then completely removed. The epithelium grew over the stroma, but haze and severe ectasia occurred. After penetrating keratoplasty, the recipient cornea was analyzed. An autopsy cornea from a person who underwent uneventful LASIK and ten normal autopsy corneas served as controls. Corneas were analyzed by immunohistochemistry. Results. Both flap regions in the treated corneas had marked alterations of ECM components and MMPs. Stromal deposits of various ECM proteins, including those normally absent in the central cornea (tenascin-C, fibrillin-1, type XIV collagen), were found. Rare myofibroblasts and inflammatory cells were present. The epithelial basement membrane (BM) was altered in both cases. The most dramatic change was poor or no staining for &agr;3–&agr;6 type IV collagen chains and thrombospondin. The limbal &agr;1–&agr;2 type IV collagen and laminin-2 (&agr;2&bgr;1&ggr;1) appeared in the central epithelial BM. Other components were altered to a lesser extent. The anterior stroma was positive for MMP-1 and MMP-2, and some MMP-7 was seen in the epithelium. These ECM and MMP patterns were not seen in uneventful LASIK or normal corneas. Conclusions. In the flaps of LASIK-treated corneas, fibrosed areas of anterior stroma had increased levels of MMP-1 and MMP-2 that may have caused loss of specific type IV collagen isoforms in the epithelial BM. These changes may reflect an ongoing wound healing process and contribute to the development of ectasia.
Exercise Training Improves Vasoreactivity in the Knee Artery

Abstract Physical activity has been shown to enhance endothelial function of central and peripheral vascular beds. The primary purpose of the present study was to test the hypothesis that a short-term exercise training program would result in enhanced endothelium-dependent vasorelaxation of a major artery supplying blood flow to the knee joint, the middle genicular artery. Female Yucatan miniature swine were randomly assigned into exercise trained (n=7) or sedentary (n=7) groups. Exercise trained pigs underwent a daily exercise training program on treadmills for 7 days. In vitro assessment of vasorelaxation was determined in a dose response manner by administrating increasing doses of 3 different dilators; adenosine diphosphate, bradykinin, and sodium nitroprusside. The role of nitric oxide synthase and cyclooxygenase pathways in vasomotor responses was evaluated with specific inhibitors using nitro-L-arginine methyl ester and indomethacin incubation, respectively. The results of this investigation indicate that adenosine and bradykinin-induced endothelium-dependent vasorelaxation were significantly enhanced in middle genicular artery from exercise trained pigs (p<0.05). Endothelium-independent vasorelaxation was not altered with exercise training as determined by the response to sodium nitroprusside. The findings of the present investigation indicate that short-term exercise training enhances endothelial function of middle genicular artery through adaptations in the nitric oxide synthase and by non-nitric oxide synthase, non-cyclooxygenase pathways.
Profound Differences in Leukocyte-Endothelial Cell Responses to Lipopolysaccharide Versus Lipoteichoic Acid1

We have investigated the effects of LPS from Escherichia coli, lipoteichoic acid (LTA), and peptidoglycan (PepG) from Staphylococcus aureus, and live S. aureus on leukocyte-endothelial interactions in vivo using intravital microscopy to visualize muscle microvasculature. Systemic vs local administration of LPS induced very different responses. Local administration of LPS into muscle induced significant leukocyte rolling, adhesion, and emigration in postcapillary venules at the site of injection. LPS given systemically dramatically dropped circulating leukocyte counts and increased neutrophils in the lung. However, the drop in circulating leukocytes was not associated with leukocyte sequestration to the site of injection (peritoneum) nor to peripheral microvessels in muscles. Unlike LPS, various preparations of LTA had no systemic and very minor local effect on leukocyte-endothelial interactions, even at high doses and for prolonged duration. LPS, but not LTA, potently activated human endothelium to recruit leukocytes under flow conditions in vitro. Endothelial adhesion molecule expression was also increased extensively with LPS, but not LTA. Interestingly, systemic administration of live S. aureus induced leukocyte-endothelial cell responses similar to LPS. PepG was able to induce leukocyte-endothelial interactions in muscle and peritoneum, but had no effect systemically (no increase in neutrophils in lungs and no decrease in circulating neutrophil counts). These results demonstrate that: 1) LPS has potent, but divergent local and systemic effects on leukocyte-endothelial interactions; 2) S. aureus can induce a systemic response similar to LPS, but this response is unlikely to be due to LTA, but more likely to be mediated in part by PepG.
Error prediction and detection methodologies for reliable circuit operation under NBTI

Aging effects, namely due to Negative Bias Temperature Instability (NBTI) produce performance degradation as the circuit is used. Consequently, the reliability of the circuit is compromised. Furthermore, increasing of process variations as technology shrinks plays an important role on the performance degradation due to aging. Even more, electronic systems for safety-critical applications must operate for many years in harsh environments. In order to guarantee a safe operation in this kind of systems, aging monitoring should be performed on-chip using built-in aging sensors. In this work, new error prediction and error detection methodologies to perform robust monitoring due to NBTI aging are proposed. A solution for error prediction using a new aging sensor is proposed. It consists of a methodology for path selection, based on statistical static timing analysis, to determine the correct location for aging sensor insertion. On the other hand, a new error detection methodology based on monitoring the actual critical paths of the circuit is proposed. This methodology has important advantages since process variations and aging degradation of the critical paths are realistically tracked. This methodology allows the same digital circuit to operate in significantly different conditions: targeting maximum performance or requiring minimum power, depending on the system status. The circuit performance can be optimized depending on the system instantaneous requirements.
Conditional RAG-1 Mutants Block the Hairpin Formation Step of V(D)J Recombination

ABSTRACT Hairpin formation serves an important regulatory role in V(D)J recombination because it requires synapsis of an appropriate pair of recombination sites. How hairpin formation is regulated and which regions of the RAG proteins perform this step remain unknown. We analyzed two conditional RAG-1 mutants that affect residues quite close in the primary sequence to an active site amino acid (D600), and we found that they exhibit severely impaired recombination in the presence of certain cleavage site sequences. These mutants are specifically defective for the formation of hairpins, providing the first identification of a region of the V(D)J recombinase necessary for this reaction. Substrates containing mismatched bases at the cleavage site rescued hairpin formation by both mutants, which suggests that the mutations affect the generation of a distorted or unwound DNA intermediate that has been implicated in hairpin formation. Our results also indicate that this region of RAG-1 may be important for coupling hairpin formation to synapsis.