Miracle-dz/UniFAR-FTUs huggingface dataset:
Game On! Preparing Your Clients for Recreational Sports

P articipating in recreational sports is frequently used to enhance and maintain fitness. Sports participation is fun, adds variety to a fitness program, and can promote exercise adherence. To enhance the sports participation experience, however, it is better for your clients to get fit before playing sports, instead of playing sports to get fit. A good conditioning program will prepare the body to make sports participation more enjoyable, prevent unnecessary soreness and stiffness that often discourages exercise participation, and help prevent exercise-related injuries. Properly conditioned individuals can safely participate in most sports and enjoy the activities to their fullest with few or no limitations. Unfortunately, sport injuries often are the result of low fitness and a lack of sport-specific conditioning. Although some injuries are the direct result of lack of fitness, many injuries occur when fatigue sets in after overexertion by unconditioned individuals. A well thought-out training program also is recommended for fitness participants who have progressed nicely in the initial weeks of a program but now feel ‘‘stale’’ and ‘‘stagnant. ’’ Studies indicate that systematically altering volume and intensity of training is most effective for longterm fitness progression. Altering workouts also helps to break staleness and the monotony of repeated workouts.
Bovine mater‐like NALP9 is an oocyte marker gene

We have cloned and sequenced the bovine NALP9 cDNA, which contains a 2991 bp long open reading frame with 76% homology to its human counterpart. The putative 996 amino acids protein presents the domain organization typical of the Nacht, Leucine rich repeat and Pyrin domain containing (NALP) family that includes the maternal effect factor MATER. By reverse transcription coupled to polymerase chain reaction, we have analyzed expression of NALP9 in a panel of somatic and gonadic tissues and during in vitro preimplantation development. The transcript was detected exclusively in testis and ovary, and at a remarkably high level in the oocyte from antral follicles. NALP9 mRNA remained present after in vitro maturation and fertilization, and was detected in embryos, but transcription was not reactivated at the maternal to embryo transition. Thus NALP9 is characterized as a novel oocyte marker gene in cattle. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.
Polyhydroxyalkanoate Degradation Is Associated with Nucleotide Accumulation and Enhances Stress Resistance and Survival ofPseudomonas oleovorans in Natural Water Microcosms

ABSTRACT Pseudomonas oleovorans GPo1 and its polyhydroxyalkanoic acid (PHA) depolymerization-minus mutant, GPo500 phaZ, residing in natural water microcosms, were utilized to asses the effect of PHA availability on survival and resistance to stress agents. The wild-type strain showed increased survival compared to the PHA depolymerase-minus strain. The appearance of a round cellular shape, characteristic of bacteria growing under starvation conditions, was delayed in the wild type in comparison to the mutant strain. Percent survival at the end of ethanol and heat challenges was always higher in GPo1 than in GPo500. Based on these results and on early experiments (H. Hippe, Arch. Mikrobiol. 56:248–277, 1967) that suggested an association of PHA utilization with respiration and oxidative phosphorylation, we investigated the association between PHA degradation and nucleotide accumulation. ATP and guanosine tetraphosphate (ppGpp) production was analyzed under culture conditions leading to PHA depolymerization. A rise in the ATP and ppGpp levels appeared concomitant with PHA degradation, while this phenomenon was not observed in the mutant strain unable to degrade the polymer. Complementation of the phaZ mutation restored the wild-type phenotype.
Cloud gateway operation system for E-commerce consolidation

E-commerce consolidation is a critical process especially in highly automated E-commerce distribution center (ECDC). Labor-based consolidation operation is flexible to suit different consolidation scenarios, but is easily to become the bottleneck of the whole process. The boom of IoT technology brings multiple assistive methods for optimization. However, the integration and deployment for IoT technology with high flexibility and convenience for enterprise users are practical but yet troublesome. To solve this problem, this paper proposes a cloud gateway operation system (CGOS). It is an overall cloud services including the front-line devices integration, network establishment, computing and storage resources allocation and the workflow management for consolidation operations.
Marketing Social Marketing: Getting Inside Those “Big Dogs’ Heads” and Other Challenges

Social marketing provides a powerful process for planning and implementing public health programs. Although often applied to the promotion of healthier lifestyles, social marketing can also be used to promote utilization of direct services or policy changes. Despite growing popularity among public health professionals, resistance by senior management, community advocates, policy makers, and others can create barriers to the use of the social marketing model. This article draws on the authors’ observations, practice experiences, extensive training interactions, and qualitative studies with public health practitioners across the nation. It examines some of the key reasons that public health practitioners encounter resistance to using social marketing and discusses how a logic model can be used to market social marketing in organizations and communities.
Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera

The western corn rootworm (WCR), a major pest of maize, is notorious for rapidly adapting biochemically, behaviourally and developmentally to a variety of control methods. Despite much effort, the genetic basis of WCR adaptation remains a mystery. Since transformation‐based applications such as transposon tagging and enhancer trapping have facilitated genetic dissection of model species such as Drosophila melanogaster, we developed a germline‐transformation system for WCR in an effort to gain a greater understanding of the basic biology of this economically important insect. Here we report the use of a fluorescent‐marked Minos element to create transgenic WCR. We demonstrate that the transgenic strains express both an eye‐specific fluorescent marker and piggyBac transposase. We identified insertion‐site junction sequences via inverse PCR and assessed insertion copy number using digital droplet PCR (ddPCR). Interestingly, most WCR identified as transgenic via visual screening for DsRed fluorescence proved to carry multiple Minos insertions when tested via ddPCR. A total of eight unique insertion strains were created by outcrossing the initial transgenic strains to nontransgenic WCR mates. Establishing transgenic technologies for this beetle is the first step towards bringing a wide range of transformation‐based tools to bear on understanding WCR biology.
Automatic extraction of the control flow machine and application to evaluating coverage of verification vectors

Simulation is still the primary, although inadequate, resource for verifying the conformity of a design to its functional specification. Fortunately, most errors in the early stages of design involve only the control flow in the circuit. We define the functional coverage of a given sequence of verification vectors as the amount of control behavior exercised by them. We present a novel technique for automatically extracting the control flow of a design on the basis of the underlying mathematical model. Significantly, this extraction is independent of the circuit description style. The Extracted Control Flow Machine (ECFM) is then used for estimation of functional coverage and to provide information that will help the designer improve the quality of his or her tests.
Collaborative forecasting: a selection of practical approaches

The Collaborative Planning Forecasting and Replenishment (CPFR) process model developed by the Voluntary Inter-industry Commerce Standards (VICS) association has received significant attention from both practitioners and academics. However, despite promising pilots, the adoption rate of CPFR has been slower than expected, especially in Europe. The reason seems to be that the proposed collaboration process is currently too labour-intensive for many companies. There is a need for streamlined approaches to get collaboration started in Europe. This paper presents a case study involving a supplier and a retailer collaborating to increase the retailer's forecasting accuracy for new product introductions. The approach is based on creating one forecast that is then shared within the supply chain. Three other streamlined approaches to planning and forecasting collaboration are also presented on a more general level. Finally, there is a discussion as to how the product life cycle model can be used to select and combine the most suitable approaches to collaboration in different market situations.
User involvement in the development of wireless applications in supply chains of the forestry industry

This study investigated how different users are involved in the development of new wireless applications for enterprises, here focusing on supply chain applications in the forestry industry. This article analyses the purpose, intensity and mode of user involvement when developing new wireless solutions by enterprise customers in close cooperation with solution suppliers. The article provides a theoretical overview of the research development of user involvement, with a focus on theories and concepts related to the customer involvement. An empirical case study forms the foundation for the analysis and discussion. Our investigation of the mode, intensity and purpose during the process revealed that user involvement can be described as a combination of commitment and participation components that there is an inverse relationship between the intensities of commitment and participation, and finally that user involvement can reduce uncertainties at the enabling, commissioning and impact levels.
Cloning of urease gene sequences from Providencia stuartii

Providencia stuartii was the most prevalent isolate recovered from urine specimens taken weekly over a 1-year period from 51 nursing home patients with urinary catheters in place. Thirty percent of the isolates were urease positive. Urease, which is implicated in renal stone formation, was shown to be transmissible on an 82-kilobase conjugative plasmid in one isolate. Plasmid DNA isolated from this strain was digested with EcoRI, ligated into the EcoRI site of pBR322, and used to transform Escherichia coli HB101. Ampicillin-resistant clones were replica plated onto urea segregation agar, and a urease-positive clone, designated pMID101, was isolated. Recombinant and native urease from cell lysates had identical electrophoretic mobilities on nondenaturing polyacrylamide urease activity gels. The native enzyme was induced fourfold when cells were grown in the presence of 0.1% urea and had a km of 9.4 mM and a Vmax of 3.2 mumol of NH3 per min per mg of protein. Its molecular weight was estimated to be 375,000 +/- 35,000 by Sephacryl S-300 chromatography. The enzyme was cytoplasmic in P. stuartii, was inhibited in vitro by hydroxyurea, acetohydroxamic acid, and EDTA, and appears to have a complex subunit structure and a unique molecular size within genera of the Proteeae tribe.
Creativity Workshops in Product Innovation

The process of creative thinking has long been a concern of both researchers and managers alike. Recently, the business press has begun to address a variety of approaches being taken to enhance creativity. One of the current structures which incorporates the use of a variety of specific techniques is the Creativity Workshop. It structures a collective group effort designed to solve a complex problem. Horst Geshka has led a number of Creativity Workshops and has summarized his experiences for the growing number of managers who recognize the importance of giving special attention to the process of creative thinking.
RNAi microarray by reverse transfection of siRNA and shRNA for functional genomics

RNA interference (RNAi) is a widely used method for gene function analyses in a variety of organisms. Establishment of a large-scale, high-throughput platform for analysing gene function may require development of an RNAi microarray system using reverse transfection. Here, we show that shRNA is more effective in microarray gene silencing compared to siRNA. Endogenous mammalian genes are suppressed efficiently if highly functional class I sequences were used for siRNAs produced via shRNAs. Furthermore, the method appears applicable for functional genomics. Indeed, apoptotic cell death induced by staurosporine and actinomycin D was found to be blocked by shRNA against apoptotic protease-activating factor-1 and caspase-3, both of which are required in the cytochrome c-dependent apoptosis cascade.
High speed 2-phase 4/3 switched reluctance motor for air-blower application: Design, analysis, and experimental verification

The mechanical structure of the 2-phase 4/3 switched reluctance motor (SRM) with a non-uniform air gap make it suitable for unidirectional rotation applications such as air-blower. The non-uniform air gap is applied on the rotor shape to increase the positive torque region, and makes the motor can be operated at any rotor position without torque dead-zone. The 4/3 SRM is employed 4-stator and 3-rotor poles. The motor stator is separated with two segmental C-cores that used combination with 3-rotor poles form the flux path in a shortest closed loop. Short flux path technique helps the motor reduce the core losses while increase the efficiency. In order to verify the design, a prototype was manufactured for laboratory test.
&ggr;-H2AX Foci as a Biomarker for Patient X-Ray Exposure in Pediatric Cardiac Catheterization: Are We Underestimating Radiation Risks?

Background— A better knowledge of patient x-ray dose and the associated radiation risk in pediatric interventional cardiology is warranted in view of the extensive use of x-rays and the higher radiosensitivity of children. In the present study, &ggr;-H2AX foci were used as a biomarker for radiation-induced effects. Patient-specific dose was assessed and radiation risks were estimated according to the linear-no-threshold model, commonly used in radiation protection, and the &ggr;-H2AX foci data. Methods and Results— In 49 pediatric patients (median age, 0.75 years) with congenital heart disease who underwent cardiac catheterization procedures, blood samples were taken before and shortly after the procedure. &ggr;-H2AX foci were determined in peripheral blood T lymphocytes. In each patient, a net increase in &ggr;-H2AX foci, representing DNA double-strand breaks induced by interventional x-rays, was observed. In addition, a patient-specific Monte Carlo simulation of the procedure was performed, resulting in individual blood, organ, and tissue doses. Plotting of &ggr;-H2AX foci versus blood dose indicated a low-dose hypersensitivity. Median effective doses calculated according to the International Commission on Radiological Protection 60 and 103 publications are 5.6 and 6.4 mSv, respectively. The lifetime-attributable risk of cancer mortality was calculated from the linear-no-threshold model and the &ggr;-H2AX foci data. This resulted in lifetime-attributable risk values of 1 per thousand and 4 per thousand, respectively, for the patient population under study. Conclusions— &ggr;-H2AX foci as a biomarker for DNA damage indicate that radiation risk estimates according to the linear-no-threshold hypothesis are possibly underestimates. Great care should be taken to minimize and optimize patient radiation exposure.
Vaccine access and affordability in a coordinated market under stochastic reservation prices

Ensuring vaccine affordability and accessibility are major challenges for the fulfillment of the immunization goals of the Global Vaccine Action Plan for the decade 2011 – 2020. Four of the five immunization goals in this plan are currently delayed because of limitations in vaccine affordability. The use of combination vaccines can help to make vaccines more accessible as long as their pricing becomes more affordable. Procurement echanisms, such as tiered pricing and pooled procurement, have generally been used for reducing the cost of vaccine purchases, but they have not been used to ensure overall market affordability. This study extends the Antigen Bundle Pricing Problem to analyze a coordinated vaccine market and understand the effects on vaccine affordability of three factors: uncertainty regarding vaccine reservation prices, number of market segments in which customers are grouped, and the interest rate at which manufacturers recover their investment. Experimental results show that increasing the number of market segments, in which countries are grouped, positively influences vaccine affordability but reduces overall profit for manufactures. This study proposes strategies to mitigate the impact to manufacturers by determining the optimal market segmentation of target countries while considering limited access to external funding.
THE EXCRETION OF LIPID AND LIPID SUBSTANCES IN HUMAN SWEAT. REP 280.

Abstract : The primary objective of this study was to determine whether the losses of lipid substances in sweat, under conditions of profuse sweating, were of significance. Under the conditions of this study at high enviromental temperatures, the total excretion of lipid substances in sweat was low and would not seem to be of consequence in studies of lipid metabolism. During a 7 1/2 hour exposure period at high temperatures, the total lipid excretion in sweat ranged between 25 and 46 mg. As one would expect, since the total lipid excretion in sweat was low, the excretion of cholesterol, free and total, and lipid phosphorus were also low. (Author)
Yellow-breasted Chat and Gray Catbird Productivity in a Fragmented Western Riparian System

Abstract We studied the effects of habitat fragmentation on productivity of Yellow-breasted Chat (Icteria virens) and Gray Catbird (Dumetella carolinensis) in southern British Columbia in a western riparian ecosystem. Nesting dates were later in isolated habitat patches than in continuous habitat patches for both species. We found no direct evidence that habitat fragmentation decreased productivity in either species. Average fecundity did not significantly differ between continuous (2.54 fledglings for Gray Catbird; 1.78 and 1.67 fledglings for Yellow-breasted Chat in 2002 and 2003, respectively) and isolated sites (1.33 fledglings for Gray Catbird; 1.78 and 0.87 fledglings for Yellow-breasted Chat in 2002 and 2003, respectively). Territory size, as measured by mapping perch locations for breeding adults, was smaller for Yellow-breasted Chats breeding in the Okanagan Valley (0.25 ha) than for chats in mid and high-density southern populations. However, overall fecundity and nest success were similar to more southerly populations. These results suggest that both species can persist in a relatively fragmented ecosystem.
Shopping orientations and patronage preferences for internet auctions

Purpose – The purpose of this paper is to analyze the effect of consumers’ shopping orientations and income on online auction patronage intentions. Design/methodology/approach – The study collected data using a national sample of 3,000 online auction consumers over the age of 18, who had purchased from internet auction sites eBay, Yahoo, or Bargain Hunter in the previous six months and used structural equation modelling for data analysis. Findings – The results suggest that consumers’ price consciousness shopping orientation, which increases shopping in online stores, decreases patronage intentions towards the online auction site. In addition, the findings indicate that consumers’ search orientation, convenience orientation and income level increase patronage intentions towards the online auction site. Originality/value – The key theoretical and academic contribution of this study focuses on testing and integrating the shopping orientations literature in the more modern area of online auction retailing. P.
Is Privatization a Socially Responsible Reform?

We assess the link between corporate social responsibility (CSR) and government ownership using a unique sample of privatized firms (PFs) from 41 countries over the 2002 to 2014 period. We find that PFs have, on average, better CSR intensity than other publicly listed firms. Further tests show a nonlinear relation between residual state ownership and CSR intensity that depends on the trade-off between political objectives of the government and profit maximization objectives of private owners. In addition, country-level institutions affect the state ownership–CSR intensity relation, and PFs benefit from higher valuation and lower equity financing costs through improved CSR.
Early osteolysis following total hip arthroplasty with use of a Hylamer liner in combination with a modular ceramic femoral head. A case report.

In an effort to improve the surface and wear characteristics of ultra-high molecular weight polyethylene, an enhanced ultra-high molecular weight polyethylene (Hylamer; DePuy-DuPont Orthopaedics, Wilmington, Delaware)6 was introduced in 1991. It was anticipated that the wear characteristics of a Hylamer acetabular surface in combination with a ceramic modular femoral head would improve the long-term performance of prostheses in young, active patients4,25. Several recent reports, however, have described wear and early osteolysis in association with Hylamer acetabular liners coupled with cobalt-chromium modular femoral heads manufactured by various companies3,15. The purpose of the current report is to describe rapid wear and early osteolysis following use of a Hylamer acetabular liner in combination with a ceramic femoral head made by the same manufacturer. In April 1994, a total hip arthroplasty was performed on a thirty-three-year-old woman (height, 167 centimeters; weight, fifty-two kilograms) who had arthritis due to congenital dysplasia of the left hip. The patient's preoperative work activities as an accountant had required frequent climbing and descending of multiple sets of stairs, which had greatly aggravated the problems with the hip. The work environment subsequently was modified to better accommodate the patient. The arthroplasty was performed with use of components from the same manufacturer. These included a femoral component (Stability; DePuy Orthopaedics, Warsaw, Indiana) measuring twelve by 150 millimeters, which was inserted without cement; an acetabular shell (Duraloc; DePuy Orthopaedics) with a diameter of fifty-two millimeters, also inserted without cement; a Ti-6Al-4V bone-screw (Low Profile; DePuy Orthopaedics) with a diameter of 6.5 millimeters and a length of thirty millimeters, for augmentation of the cup; and a neutral Hylamer liner measuring fifty-two by twenty-eight millimeters with a thickness of eight millimeters. Because of the patient's young age, activity level, and life expectancy, an aluminum-oxide-ceramic modular …