Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of Allergenicity of Acid-Hydrolyzed Wheat Protein Using an in vitro Elicitation Test

Background: We performed an in vitro elicitation test to determine the ability of different types of wheat-allergic patients’ IgE to induce humanized mast cell activation after the addition of various time-treated acid-hydrolyzed wheat proteins (HWPs). Methods: The reactivity of heat- and various time-treated acid-hydrolyzed glutens (acid-HGs) and commercial acid-HWP (HWP1), using serum IgE from wheat allergy accompanied by skin and rhinoconjunctival sensitization to HWP1 in the facial soap, pediatric subjects with food allergy to native wheat, adult wheat-dependent exercise-induced anaphylaxis subjects, and nonatopic healthy subjects, was elucidated by dot blot and a luciferase assay-based in vitro elicitation test (EXiLE test). Results: Serum from subjects sensitized with HWP1 reacted only to acid-HGs (acid-HGs treated for 0.5–3 or 6 h), but not native gluten, in the results of the dot blot. In contrast, sera from pediatric subjects sensitized with native wheat reacted to native gluten more strongly and showed only slight reactions to 0.5- to 1-hour-treated acid-HGs. The results of the in vitro elicitation test showed that acid hydrolyzation of the gluten attenuated antigen-induced luciferase expression in a time-dependent manner for sera from native-wheat-sensitized pediatric subjects. On the other hand, in the sera from HWP1-sensitized subjects, acid hydrolyzation of the gluten for 0.5 h dramatically increased luciferase expression. Conclusions: Even after prolonged hydrolyzation, acid-HGs still retained the ability to activate mast cells in the case of HWP1-sensitized subjects.
Integration of Planar Antennas Considering Electromagnetic Interactions at Board Level

In compact wireless modules, electromagnetic (EM) interactions occurring between planar antennas and transmission lines (TML) sharing the same substrate may cause a high amount of undesired coupling and may also detune the antenna characteristics. In this paper, an approach for defining a block-out region around the planar antenna, where no components should be placed is developed, thereby ensuring that the antenna characteristics remain within tolerable limits when the antenna is integrated at board level. This region is comparable to the reactive near-field, but is determined by evaluating the reactive EM power density excited on the ground plane and deducing a threshold value. Its boundary will be termed the EM antenna boundary. Furthermore, a method for efficient estimation of EM coupling from the antenna to terminated TMLs routed outside the EM antenna boundary is developed. This method is based entirely on a postprocessing step to field simulations, i.e., the coupling is calculated based on the previously computed magnetic field distribution excited by the antenna on the ground plane. The coupling model uses the theory of field excited TMLs together with the Baum-Liu-Tesche integral equations for obtaining the terminal voltages of the TML and, hence, the coupling terms.
Contributions of cat posterior parietal cortex to visuospatial discrimination

The purpose of the present study was to examine the contributions made by cat posterior parietal cortex to the analyses of the relative position of objects in visual space. Two cats were trained on a landmark task in which they learned to report the position of a landmark object relative to a right or left food-reward chamber. Subsequently, three pairs of cooling loops were implanted bilaterally in contact with visuoparietal cortices forming the crown of the middle suprasylvian gyrus (MSg; architectonic area 7) and the banks of the posterior-middle suprasylvian sulcus (pMS sulcal cortex) and in contact with the ventral-posterior suprasylvian (vPS) region of visuotemporal cortex. Bilateral deactivation of pMS sulcal cortex resulted in a profound impairment for all six tested positions of the landmark, yet bilateral deactivation of neither area 7 nor vPS cortex yielded any deficits. In control tasks (visual orienting and object discrimination), there was no evidence for any degree of attentional blindness or impairment of form discrimination during bilateral deactivation of pMS cortex. Therefore, we conclude that bilateral cooling of pMS cortex, but neither area 7 nor vPS cortex, induces a specific deficit in spatial localization as examined with the landmark task. These observations have significant bearing on our understanding of visuospatial processing in cat, monkey, and human cortices.
Quetiapine as an adjunctive treatment for post-traumatic stress disorder: an 8-week open-label study

This study evaluated the effectiveness of quetiapine for subjects with post-traumatic stress disorder (PTSD) who were already on a stable dose of a selective serotonin reuptake inhibitor (SSRI) but had significant PTSD symptoms. Fifteen subjects were enrolled in an 8-week open-label trial for PTSD in which quetiapine was added to an SSRI. Subjects were on a stable dose of the SSRI for at least 6 weeks before study entry and had a Clincian-Administered PTSD Scale (CAPS) score of greater than or equal to 50 at study baseline. The mean age of subjects was 49 years (eight men and seven women). The average duration of PTSD was 29 years, one-third of subjects had combat-related PTSD, and two-thirds had noncombat PTSD. The mean dose prescribed in the study was 216 mg per day. The initial median CAPS score was 80, indicating severe PTSD. The addition of a modest dose of quetiapine provided significant relief from PTSD symptoms with a 42% overall improvement in PTSD symptoms based on the CAPS and significant improvement along each dimension of symptoms: re-experiencing (Z=−3.24, P=0.0012), hyperarousal (Z=−3.30, P=0.001) and avoidance (Z=−2.13, P=0.03). Subjects rated themselves as 45% improved on average on the Davidson Trauma Scale and reported a 44% decrease in their level of disability and impairment as reflected by the Sheehan Disability Scale. Subjects with PTSD who had significant PTSD symptoms when on an SSRI benefited from the addition of quetiapine. Patients improved significantly on all three clusters of PTSD symptoms: re-experiencing, hyperarousal and avoidance.
Endogenous ligands of carbohydrate recognition domains of the mannose receptor in murine macrophages, endothelial cells and secretory cells; potential relevance to inflammation and immunity

The macrophage mannose receptor (MR) has an established role in the phagocytosis of a wide range of microbes, and also functions in viral endocytosis, and clearance of a number of endogenous glycoproteins from the circulation. Its broad ligand specificity is mediated by tandemly linked carbohydrate recognition domains (CRDs). Recent studies suggest that binding or internalization of bothnatural and synthetic ligands of MR CRDs may modulate macrophage (MΦ ) function, for example to increase cidal capacity or cytokine synthesis. To identify endogenous ligands in the normal mousewe used an Fc‐fusion protein (CRD4‐7Fc) bearing four of the CRDs of MR. CRD4‐7Fc recognized endocytic compartments of cultured MΦ , consistent with lysosomal enzymes being major ligands of MR. CRD4‐7Fc also recognized MΦ and some endothelial cells in tissues, and intensely labeled secretory cells of the exocrine pancreas, salivary gland and thyroid. Strongly MR‐positive interstitial cells were found in close proximity to the ligand‐rich secretory cells, suggesting a role for MR in uptake of secretory glycoproteins, including thyroglobulin which was identified as a novel ligand in vitro. Endocytosis of these ligands by MR may have implications for tissue homeostasis and immunity, including antigen presentation, in secretory organs.
Biotechnologically Relevant Enzymes and Proteins

High stability and low competitive inhibition of thermophilic Thermopolyspora flexuosa GH10 xylanase in biomass-dissolving ionic liquids Abstract Thermophilic Thermopolyspora flexuosa GH10 xylanase (TfXYN10A) was studied in the presence of biomass-dissolving hydrophilic ionic liquids (ILs) [EMIM]OAc, [EMIM]DMP and [DBNH]OAc. The temperature optimum of TfXYN10A with insoluble xylan in the pulp was at 65–70 °C, with solubilised 1 % xylan at 70–75 °C and with 3 % xylan at 75–80 °C. Therefore, the amount of soluble substrate affects the enzyme activity at high temperatures. The experiments with ILs were done with 1 % substrate. TfXYN10A can partially hydro-lyse soluble xylan even in the presence of 40 % (v/v) ILs. Although ILs decrease the apparent temperature optimum, a surprising finding was that at the inactivating temperatures (80–90 °C), especially [EMIM]OAc increases the stability of TfXYN10A indicating that the binding of IL molecules strengthens the protein structure. Earlier kinetic studies showed an increased K m with ILs, indicating that ILs function as competitive inhibitors. TfXYN10A showed low increase of K m , which was 2-, 3-and 4-fold with 15 % [EMIM]OAc, [DBNH]OAc and [EMIM]DMP, respectively. One reason for the low competitive inhibition could be the high affinity to the substrate (low K m). Xylanases with low K m (~1 mg/mL) appear to show higher tolerance to ILs than xylanases with higher K m (~2 mg/mL). Capillary electrophoresis showed that TfXYN10A hydrolyses xylan to the end-products in 15–35 % ILs practically as completely as without IL, also indicating good binding of the short substrate molecules by TfXYN10A despite of major apparent IL binding sites above the catalytic residues. Substrate binding interactions in the active site appear to explain the high tolerance of TfXYN10A to ILs.
Learning And Developing From Managerial Work Experiences

DURING the last decade there has been an increasing interest in the waymanagers learn from normal work experiences. This has emerged fromscepticism about whether formal management training ever gets used at all(Davies, 1973; Mant, 1969), and questions about whether, even if it is used,it produces the kind of behaviour which might lead to effective management,and hence business, performance (Hays and Abernathy, 1980; Livingston,1971; Revans, 1981). Furthermore the potency of natural learning has beenemphasized at a theoretical level by Kolb e<a/. (1971), and at a practical levelby a number of writers (Hague, 1979; Mumford, 1980, 1981). Above all,natural learning, and self-development with which it is associated, has thevirtue of being relatively cheap to foster - provided this is done effectively(Burgoyne, 1977). The problem addressed in this paper is that of ensuringthat managers do in fact learn and develop as much as they might from theirnormal work experiences. The bulk of the paper is based on an analysis of theresults of some 60 interviews carried out by the authors with middle andsenior managers about their own developmental experiences. The managerswere mainly in their late 3O's or early 4O's and both line and functionalspecialists were represented in our sample. The paper concludes with someobservations about the limitations of existing theories of 'experientiallearning', when applied to management development, and makes somesuggestions about adapting existing organizational policies for developingmanagers accordingly.
Voting Behaviour among the Gay, Lesbian, Bisexual and Transgendered Electorate

Abstract. The gay, lesbian, bisexual and transgendered (GLBT) population is a good example of a demographic group that has been understudied because it is difficult to develop a subsample of sufficient size from typical national samples. Here we exploit the extraordinary size of a 2006 online election day survey (with about 35,000 respondents) to examine how the GLBT community behaves politically. While it will surprise no one that this community bestowed little support on Stephen Harper's Conservative party in the 2006 federal election, the factors behind such a consistent vote pattern are not adequately understood. In order to shed more light on the voting behaviour of the GLBT electorate, we develop a socio-demographic profile of the group, and explore three explanatory angles: 1) salience of issue campaign dynamics, given that the same-sex marriage issue was prominent in 2006; 2) ideological and attitudinal proclivities; and 3) strategic considerations. Résumé. La population gaie, lesbiennes, bisexuels et transgenres (GLBT) est un exemple d'un groupe démographique qui a été peu étudié, car il est difficile de développer un sous-échantillon de taille suffisante à partir d'échantillons nationaux. Ici, nous exploitons la taille extraordinaire d'une enquête enligne du jour du scrutin fédérale du 2006 (avec environ 35.000 répondants) d'examiner comment la communauté GLBT se comporte politiquement. Bien qu'il ne surprendra personne que cette communauté accordé peu d'appui sur Parti conservateur de Stephen Harper lors de l'élection fédérale de 2006, les facteurs qui expliquent un tel motif ne sont pas bien compris. Afin de jeter plus de lumière sur le comportement de vote de l'électorat GLBT, nous développons un profil sociodémographique de cette groupe, et d'explorer trois angles explicatives: 1) pertinence de la question du mariage de même sexe, 2) tendances idéologiques, et 3) des considérations stratégiques.
Q-enhancement of a low-power gm-C bandpass filter for closed-loop sensor readout applications

In this paper, a Q-enhancement technique for gm-C biquadratic bandpass filters is discussed. The frequencies of the parasitic pole-zero pair of a low-transconductance OTA are altered with an additional compensation capacitor, which cancels the nonidealities of the resonator. The presented technique is used to design a resonator which fulfills the requirements on a loop-filter for closed-loop delta-sigma sensor readout applications. The high stability of the Q-factor against transconductance and center frequency tuning, as well as against process variations is discussed and demonstrated with transistor-level simulations. The designed resonator exhibits a 3-sigma worst-case Q-factor larger than 3600 at the nominal center frequency of 25 kHz and a Q-factor larger than 1000 over the tuning range from 13 kHz to 32 kHz.
Indexing evidential data

Querying imperfect data received increasing attention in the area of database management. The complexity and the volume of imperfect data requires advanced techniques for efficient access, and satisfying user-queries in a reasonable response time. In this paper, we are particularly interested in evidential databases, i.e., databases where imperfection is represented through Dempster-Shafer theory. To answer user-queries in such databases, we propose a new index system based on a tree data structure (e-Tree) that is adapted to the complexity of the evidential data. The experiments done on our solution showed encouraging results.
Practice of Epidemiology Guillain-Barré Syndrome During the 2009 – 2010 H 1 N 1 Influenza Vaccination Campaign : Population-based Surveillance Among 45 Million Americans

Because of widespread distribution of the influenza A (H1N1) 2009 monovalent vaccine (pH1N1 vaccine) and the prior association between Guillain-Barré syndrome (GBS) and the 1976 H1N1 influenza vaccine, enhanced surveillance was implemented to estimate the magnitude of any increased GBS risk following administration of pH1N1 vaccine. The authors conducted active, population-based surveillance for incident cases of GBS among 45 million persons residing at 10 Emerging Infections Program sites during October 2009–May 2010; GBS was defined according to published criteria. The authors determined medical and vaccine history for GBS cases through medical record review and patient interviews. The authors used vaccine coverage data to estimate person-time exposed and unexposed to pH1N1 vaccine and calculated ageand sex-adjusted rate ratios comparing GBS incidence in these groups, as well as ageand sex-adjusted numbers of excess GBS cases. The authors received 411 reports of confirmed or probable GBS. The rate of GBS immediately following pH1N1 vaccination was 57% higher than in person-time unexposed to vaccine (adjusted rate ratio1⁄4 1.57, 95% confidence interval: 1.02, 2.21), corresponding to 0.74 excess GBS cases per million pH1N1 vaccine doses (95% confidence interval: 0.04, 1.56). This excess risk was much smaller than that observed during the 1976 vaccine campaign and was comparable to some previous seasonal influenza vaccine risk assessments.
Innovation And Job Creation In A Smallopen Economy-Evidence From Norwegian Manufacturing Plants 1982–92

It is often claimed that the opportunities to create new manufacturing jobs in open, high-cost economies such as Norway, are concentrated in activities which are technologically advanced and knowledge intensive. This paper examines the relationship between job creation and innovation, as measured by R&D investments, in Norwegian manufacturing. We compare job creation in plants belonging to R&D firms to job creation in plants belonging to firms without R&D. We also compare job creation in plants belonging to high and low tech industries. Our data set covers more than 80 percent of manufacturing employment in Norway over the period 1982-92. The paper challenges the optimistic view about job creation in R&D intensive firms and high-tech industries. Some main findings are: (i) Net job creation is not higher in high-tech industries. (ii) There is no clear-cut positive relationship between net job creation and the R&D-intensity of the firm. (iii) There is less net job creation and less job-security in R&D-intensitefirms in the late 1980s and early 1990s.
Spontaneous and induced labour are associated with different myometrial proteomes in the human

Human myometrium undergoes a major phenotypic change at labour likely involving modifications to key regulatory proteins. In some cases, the myometrium fails to activate normally and medical intervention is required to induce labour. In this study, 2‐D DIGE was used to examine changes in the myometrial proteome at the time of spontaneous (SL) and induced labour (IL). Proteomic profiles of nonlabouring term myometria (NL, n = 6) were quantitatively compared to SL (n = 6) and prostaglandin/oxytocin‐IL term myometria (n = 6). In SL samples, 23 differentially expressed protein spots were detected (9 increased/14 decreased compared to NL, p<0.05). In IL samples, 59 differentially expressed spots were observed (13 increased/46 decreased compared to NL). Comparison of SL and IL proteomes revealed 69 differentially expressed proteins (7 increased/62 decreased). Two proteins consistently decreased in SL and IL samples were identified as transgelin (1.98‐ and 1.97‐fold decrease in SL and IL, respectively) and αB‐crystallin (3.27‐ and 2.49‐fold decrease). Levels of desmin and cytosolic phospholipase A2 β were decreased 2.9‐ and 2.65‐fold, respectively only in IL samples. Our results show human labour is accompanied by general downregulation of specific myometrial proteins. Differences exist between SL and IL myometrial proteomes indicating divergence of underlying processes and highlighting the importance of distinguishing these groups in future studies of parturition. Our findings underscore the utility of discovery approaches in investigations of organ‐wide protein changes that underlie discrete physiological events including human labour.
Kinetic basis for linking the first two enzymes of chlorophyll biosynthesis

Purified recombinant proteins from Synechocystis PCC6803 were used to show that the magnesium chelatase ChlH subunit stimulates magnesium protoporphyrin methyltransferase (ChlM) activity. Steady‐state kinetics demonstrate that ChlH does not significantly alter the Km for the tetrapyrrole substrate. However, quenched‐flow analysis reveals that ChlH dramatically accelerates the formation and breakdown of an intermediate in the catalytic cycle of ChlM. In light of the profound effect that ChlH has on the methyltransferase catalytic intermediate, the pre steady‐state analysis in the current study suggests that ChlH is directly involved in the reaction chemistry. The kinetic coupling between the chelatase and methyltransferase has important implications for regulation of chlorophyll biosynthesis and for the availability of magnesium protoporphyrin for plastid‐to‐nucleus signalling.
Causes of In-migration to Tel-Aviv Inner City

Several constraints and motivations in explaining migration to the inner city of Tel-Aviv are analysed. It is argued that the process was triggered off by decline in new constructions relative to the constitution of new households in Israel. However, the back-to-the-city movement was not stimulated by a manipulative elite or by decline in housing prices in the inner city. Instead, the inner city became the preferred location of several groups of lifestyles including young urbanites, Yuppies and Dinkies, and young mobile households, as well as more family-oriented households seeking high-status flats in the inner city.
Model-driven software engineering in practice: privacy-enhanced filtering of network traffic

Network traffic data contains a wealth of information for use in security analysis and application development. Unfortunately, it also usually contains confidential or otherwise sensitive information, prohibiting sharing and analysis. Existing automated anonymization solutions are hard to maintain and tend to be outdated. We present Privacy-Enhanced Filtering (PEF), a model-driven prototype framework that relies on declarative descriptions of protocols and a set of filter rules, which are used to automatically transform network traffic data to remove sensitive information. This paper discusses the design, implementation and application of PEF, which is available as open-source software and configured for use in a typical malware detection scenario.
The nexus between education, learning, and language

Language plays a central role in cognition and learning, and language-ineducation policy determines who has access to education. Fifty years after the proclamation of independence in Africa, education is still sought through colonial languages (English, French or Portuguese), and these languages remain the key to acquiring, maintaining and enhancing a higher socio-economic and political standing. Whilst multilingualism is the most prominent feature of speech communities in Africa, the education of the African child is still locked into a monolingual policy of language substitution, a policy that imposes a language of instruction that the majority of learners do not know and that