Miracle-dz/UniFAR-FTUs huggingface dataset:
Pinhole SPECT: an approach to in vivo high resolution SPECT imaging in small laboratory animals.

UNLABELLED The performance of pinhole SPECT and the application of this technology to investigate the localization properties of radiopharmaceuticals in vivo in small laboratory animals are presented. METHODS System sensitivity and spatial resolution measurements of a rotating scintillation camera system are made for a low-energy pinhole collimator equipped with 1.0-, 2.0- and 3.3-mm aperture pinhole inserts. The spatial detail offered by pinhole SPECT for in vivo imaging was investigated in studies of the brain and heart in Fisher 344 rats by administering 201TICI, 99mTc-HMPAO, 99mTc-DTPA and 99mTc-MIBI. Image acquisition is performed using a rotating scintillation camera equipped with a pinhole collimator; projection data are acquired in conventional step-and-shoot mode as the camera is rotated 360 degrees around the subject. Pinhole SPECT images are reconstructed using a modified cone-beam algorithm developed from a two-dimensional fanbeam filtered backprojection algorithm. RESULTS The reconstructed transaxial resolution of 2.8 mm FWHM and system sensitivity of 0.086 c/s/kBq with the 2.0-mm pinhole collimator aperture provide excellent spatial detail and adequate sensitivity for imaging the regional uptake of the radiopharmaceuticals in tumor, organs and other tissues in small laboratory animals. CONCLUSION The resolution properties of pinhole SPECT are superior to those which have been achieved thus far with conventional SPECT or PET imaging technologies. Pinhole SPECT provides an important approach for investigating localization properties of radiopharmaceuticals in vivo.
The effectiveness of the stylometry of function words in discriminating between Shakespeare and Fletcher

A number of recent' successful authorship studies have relied on a statistical analysis of language features based on function words. However, stylometry has not been extensively applied to Elizabethan and Jacobean dramatic questions. To determine the effectiveness of such an approach in this field, language features are studied in twenty-four plays by Shakespeare and eight by Fletcher. The goal is to develop procedures that might be used to determine the authorship of individual scenes in The Two Noble Kinsmen and Henry VIII. Homonyms, spelling variants and contracted forms in old-spelling dramatic texts present problems for a computer analysis. A program that uses a system of pre-edit codes and replacement /expansion lists was developed to prepare versions of the texts in which all forms of common words can be recognized automatically. To evaluate some procedures for determining authorship developed by A. Q. Morton and his colleagues, occurrences of 30 common collocations and 5 proportional pairs are analyzed in the texts. Within-author variation for these features is greater than had been found in previous studies. Univariate chi-square tests are shown to be of limited usefulness because of the statistical distribution of these textual features and correlation between pairs of features. The best of the collocations do not discriminate as well as most of the individual words from which they are composed. Turning to the rate of occurrence of individual words and groups of words, distinctiveness ratios and t-tests are used to select variables that best discriminate between Shakespeare and Fletcher. Variation due to date of composition and genre within the Shakespeare texts is examined. A multivariate and distributionfree discriminant analysis procedure (using kernel estimation) is introduced. The classifiers based on the best marker words and the kernel method are not greatly affected by characterization and perform well for samples as short as 500 words. When the final procedure is used to assign the 459 scenes of known authorship (containing at least 500 words), ""*-` 1795% are assigned to C"orrect author. Only two scenes are incorrectly classified, and 4.5% of the scenes cannot be assigned to either author by the procedure. When applied to individual scenes of at least 500 words in The Two Noble Kinsmen and Henry VIII, the procedure indicates that both plays are collaborations and generally supports the usual division. However, the marker words in a number of scenes often attributed to Fletcher are very much closer to Shakespeare's pattern of use. These scenes include TNK W.iii and H8 I.iii, IV.i—ii and V.iv. To the memory of my mother, who always believed in the members of her family, even when we ourselves doubted.
Implementation of multi-criteria collaborative filtering on cluster using Apache Spark

Scalability is a problem commonly faced by a recommendation system that uses collaborative filtering methods. Multi-criteria collaborative filtering recommender system has the exact same problem. The performance of multi-criteria collaborative filtering is reduced when the amount of data processed by recommender system is increasing too high. This research aims to complement previous research which is to improve the scalability of multi-criteria collaborative filtering recommender system by applying scale-out approach or adding computer node to run the recommender system. The process of generating a recommendation on multi-criteria collaborative filtering recommender system will be done on multiple nodes of computer network inside a cluster using Apache Spark framework. To measure system scalability, the running time of multi-criteria collaborative filtering recommender system that are implemented as a recommender program on Apache Spark cluster will be compared in the form of speedup value. Based on test results, it is known that multi-criteria collaborative filtering on Apache Spark cluster has better running time than its sequential counterpart. Unfortunately, as the numbers of nodes inside cluster are increased, multi-criteria collaborative filtering recommender system on Apache Spark cluster does not gain ideal speedup.
Parameter extraction of composite right / left handed (CRLH) transmission line unit cell using off resonance method

This paper presents a novel technique for parameter extraction of a CRLH unit cell using off resonance method. The classical technique results in a set of four undetermined equations with six unknowns. Two additional equations are then obtained by differentiation, thus requiring the solution of non-homogeneous differential equations. In this paper we propose an off resonance method that uses linear approximation along with a de-embedded two port measurement to obtain closed form equations independent of derivatives. The procedure is then verified by parameter extraction of a CRLH unit cell available in literature, operating at 3.9 GHz. The proposed method gives a simple and efficient procedure for parameter extraction of the CRLH unit cell for metamaterial based microwave applications.
Regulation of nonsmall‐cell lung cancer stem cell like cells by neurotransmitters and opioid peptides

Nonsmall‐cell lung cancer (NSCLC) is the leading type of lung cancer and has a poor prognosis. We have shown that chronic stress promoted NSCLC xenografts in mice via stress neurotransmitter‐activated cAMP signaling downstream of beta‐adrenergic receptors and incidental beta‐blocker therapy was reported to improve clinical outcomes in NSCLC patients. These findings suggest that psychological stress promotes NSCLC whereas pharmacologically or psychologically induced decreases in cAMP may inhibit NSCLC. Cancer stem cells are thought to drive the development, progression and resistance to therapy of NSCLC. However, their potential regulation by stress neurotransmitters has not been investigated. In the current study, epinephrine increased the number of cancer stem cell like cells (CSCs) from three NSCLC cell lines in spheroid formation assays while enhancing intracellular cAMP and the stem cell markers sonic hedgehog (SHH), aldehyde dehydrogenase‐1 (ALDH‐1) and Gli1, effects reversed by GABA or dynorphin B via Gαi‐mediated inhibition of cAMP formation. The growth of NSCLC xenografts in a mouse model of stress reduction was significantly reduced as compared with mice maintained under standard conditions. Stress reduction reduced serum levels of corticosterone, norepinephrine and epinephrine while the inhibitory neurotransmitter γ‐aminobutyric acid (GABA) and opioid peptides increased. Stress reduction significantly reduced cAMP, VEGF, p‐ERK, p‐AKT, p‐CREB, p‐SRc, SHH, ALDH‐1 and Gli1 in xenograft tissues whereas cleaved caspase‐3 and p53 were induced. We conclude that stress neurotransmitters activate CSCs in NSCLC via multiple cAMP‐mediated pathways and that pharmacologically or psychologically induced decreases in cAMP signaling may improve clinical outcomes in NSCLC patients.
Isolation and Characterization of Acid Phosphatase Mutants in Saccharomyces cerevisiae

Saccharomyces cerevisiae strain H-42 seems to have two kinds of acid phosphatase: one which is constitutive and one which is repressible by inorganic phosphate. The constitutive enzyme was significantly unstable to heat inactivation, and its Km of 9.1 × 10−4m for p-nitrophenylphosphate was higher than that of the repressible enzyme (2.4 × 10−4m). The constitutive and the repressible acid phosphatases are specified by the phoC gene and by the phoB, phoD, or phoE gene, respectively. Results of tetrad analysis suggested that the phoC and phoE genes are linked to the lys2 locus on chromosome II. Since both repressible acid and alkaline phosphatases were affected simultaneously in the phoR, phoD, and phoS mutants, it was concluded that these enzymes were under the same regulatory mechanism or that they shared a common polypeptide. The phoR mutant produced acid phosphatase constitutively, and the phoR mutant allele was recessive to its wild-type counterpart. The phoS mutant showed a phenotype similar to that of a mutant defective in one of the phoB, phoD, or phoE genes. However, the results of genetic analysis of the phoS mutant clearly indicated that the phoS gene is not a structural gene for either of the repressible acid and alkaline phosphatases, but is a kind of regulatory gene. According to the proposed model, the phoS gene controls the expression of the phoR gene, and inorganic phosphate would act primarily as an inducer for the formation of the phoR product which represses phosphatase synthesis.
Joint SOGC/CSEP clinical practice guideline: exercise in pregnancy and the postpartum period.

OBJECTIVE To design Canadian guidelines advising obstetric care providers of the maternal, fetal, and neonatal implications of aerobic and strength-conditioning exercises in pregnancy. OUTCOMES Knowledge of the impact of exercise on maternal, fetal, and neonatal morbidity, and of the maternal measures of fitness. EVIDENCE MEDLINE search from 1966 to 2002 for English-language articles related to studies of maternal aerobic and strength conditioning in a previously sedentary population, maternal aerobic and strength conditioning in a previously active population, impact of aerobic and strength conditioning on early and late pregnancy outcomes, impact of aerobic and strength conditioning on neonatal outcomes, as well as for review articles and meta-analyses related to exercise in pregnancy. VALUES The evidence collected was reviewed by the Society of Obstetricians and Gynaecologists of Canada (SOGC Clinical Practice Obstetrics Committee) with representation from the Canadian Society for Exercise Physiology, and quantified using the evaluation of evidence guidelines developed by the Canadian Task Force on the Periodic Health Exam.
Splenectomy for Thrombocytopenia in Patients With Hepatitis C Cirrhosis

Background Many patients with hepatitis C cirrhosis and low Model for End-Stage Liver Disease scores are too early for transplant but too thrombocytopenic to treat with interferon. Goal To report a case series of splenectomy to raise platelet counts so that pegylated interferon and ribavirin can be given in patients with hepatitis C cirrhosis. Methods Retrospective chart and computer record review. Results Seven patients with hepatitis C cirrhosis (mean age=45.4±11.1 y, 4 men) had elective splenectomy for thrombocytopenia before pegylated interferon-α 2b therapy. All had thrombocytopenia contraindicating antiviral therapy. Five were Child's-Pugh Class A; 2 were B. All 7 had increases in platelet count (mean 32,400 to 222,140 /mL, P<0.01) at 221±151 days postsurgery. Median hospital stay and blood loss were 9 days (4 to 25) and 750 mL (100 to 2500 mL). Median platelet packs, units of packed red blood cells and fresh frozen plasma given were 1 (0 to 7), 0 (0 to 14) and 0 (0 to 2), respectively. There were no deaths or portal vein thrombosis. One patient who was status-post liver transplantation had significant morbidity. Five completed pegylated interferon-α 2b and ribavirin therapy; 1 is on therapy and 1 awaits initiation. Of the 5 who completed therapy, there were 2 with sustained virologic response, 1 nonresponse, 1 breakthrough, and 1 relapse. Conclusions Splenectomy in patients with hepatitis C cirrhosis can be done safely to allow application of antiviral treatment and potentially avoid transplantation. It may be considered in patients with Child's-Pugh A cirrhosis, no prior abdominal surgeries and with non-1 HCV viral genotype.
Methods to maximise recovery of environmental DNA from water samples

The environmental DNA (eDNA) method is a detection technique that is rapidly gaining credibility as a sensitive tool useful in the surveillance and monitoring of invasive and threatened species. Because eDNA analysis often deals with small quantities of short and degraded DNA fragments, methods that maximize eDNA recovery are required to increase detectability. In this study, we performed experiments at different stages of the eDNA analysis to show which combinations of methods give the best recovery rate for eDNA. Using Oriental weatherloach (Misgurnus anguillicaudatus) as a study species, we show that various combinations of DNA capture, preservation and extraction methods can significantly affect DNA yield. Filtration using cellulose nitrate filter paper preserved in ethanol or stored in a -20°C freezer and extracted with the Qiagen DNeasy kit outperformed other combinations in terms of cost and efficiency of DNA recovery. Our results support the recommendation to filter water samples within 24hours but if this is not possible, our results suggest that refrigeration may be a better option than freezing for short-term storage (i.e., 3–5 days). This information is useful in designing eDNA detection of low-density invasive or threatened species, where small variations in DNA recovery can signify the difference between detection success or failure.
Mutational Inactivation of a Gene Homologous to Escherichia coli ptsP Affects Poly-β-Hydroxybutyrate Accumulation and Nitrogen Fixation in Azotobacter vinelandii

ABSTRACT Strain DS988, an Azotobacter vinelandii mutant with a reduced capacity to accumulate poly-β-hydroxybutyrate, was isolated after mini-Tn5 mutagenesis of the UW136 strain. Cloning and nucleotide sequencing of the affected locus revealed a gene homologous to Escherichia coli ptsP which encodes enzyme INtr, a homologue of enzyme I of the phosphoenol pyruvate-sugar phosphotransferase system with an N-terminal domain similar to the N-terminal domain of some NifA proteins. Strain DS988 was unable to grow diazotrophically with 10 mM glucose as a carbon source. Diazotrophic growth on alternative carbon sources such as gluconate was only slightly affected. Glucose uptake, as well as glucose kinase and glucose-6-phosphate-dehydrogenase activities that lead to the synthesis of gluconate-6-phosphate, were not affected by the ptsP mutation. The inability of DS988 to grow diazotrophically in 10 mM glucose was overcome by supplying ammonium or other sources of fixed nitrogen. Acetylene reduction activity but not transcription of the nitrogenase structural gene nifH was shown to be impaired in strain DS988 when it was incubated in 10 mM glucose. The diazotrophic growth defect of DS988 was restored either by increasing the glucose concentration to above 20 mM or by lowering the oxygen concentration. These data suggest that a mutation inptsP leads to a failure in poly-β-hydroxybutyrate metabolism and in the respiratory protection of nitrogenase under carbon-limiting conditions.
Impact of Antidepressant Therapy on Cognitive Aspects of Work, Condom Use, and Psychosocial Well-Being among HIV Clients in Uganda

Objective: Depression has been found to impede several health outcomes among people living with HIV, but little research has examined whether depression treatment mitigates this influence. We assessed the impact of antidepressant therapy on measures of work, condom use, and psychosocial well-being among depressed HIV clients in Uganda. Methods: Paired t-tests and McNemar tests were used to assess change in survey data collected from participants at initiation of antidepressant therapy (baseline) and 6 months later. Results: Ninety-five participants completed the 6-month assessment, of whom 82 (86%) responded to treatment (defined as Patient Health Questionnaire-9 score < 5). Among study completers, work functioning improved significantly, as did measures of self-efficacy related to condom use and work (as well as general self-efficacy), and internalized HIV stigma declined; however, actual engagement in work activity and consistent condom use did not show significant change. Similar findings were observed among treatment responders. Conclusions: Antidepressant treatment benefits functional capacity, psychological well-being, and cognitive intermediary factors that may be essential for behavioral change related to work and condom use, but supplementary therapeutic strategies may be needed to impact more direct behavioral change.
The relationship between living environment, well-being and lifestyle behaviours in young women in Shiraz, Iran.

There has been increasing interest in understanding the influence of the living environment on individual and population health. While our understanding of the connection is growing, there has been limited published research focusing on socially and economically transitioning countries such as Iran or specific populations such as young women. This study explores the relationship between the physical and social living environment with well-being outcomes and lifestyle behaviours of young women in Shiraz, Iran, in 2013. Using a cluster convenience sampling technique, 391 young Iranian women with the mean age of 27.3 (SD: 4.8) participated in a cross-sectional survey (response rate 93%). A scale adapted from the British General Household Social Capital scale was used to assess living environment characteristics. The International Health and Behaviour survey, Satisfaction with Life Scale (SwL) and WHO Quality of Life questionnaire (WHOQOL-BREF) were used to measure lifestyle behaviours and well-being. The findings showed a moderate level of satisfaction with participants' living environment, with a mean score of 38.5 (SD: 7.7; score range: 11-45). There were correlations between physical and social neighbourhood environment, lifestyle behaviours and well-being outcomes (P < 0.05). Multiple regression analysis showed that the characteristics of living environments were determinants of quality of life (QoL), including physical, psychological, social and environmental QoL, as well as SwL (P < 0.05). Perceptions of individuals about their living environment issues were associated with demographic variables including ethnicity, income, level of education and occupation status. The current study shows how characteristics of the physical and social living environments play a significant role in shaping well-being and lifestyle behaviours among young Iranian women. Hence, there is a need for more focused attention to the meaning, measurement and building of neighbourhood livability, including both physical and social aspects of neighbourhood, in order to support QoL and SwL among young Iranian women, and enhance their healthy lifestyle behaviours.
Distinct patterns of apoptosis in association with modulation of CD44 induced by thrombopoietin and granulocyte‐colony stimulating factor during ex vivo expansion of human cord blood CD34+ cells

The insufficient number of haemopoietic stem cells (HSCs) in cord blood (CB) is the major potential limitation to widespread use of CB for marrow replacement. Cytokine‐mediated ex vivo expansion has been proposed as a means of increasing the number of CB HSCs for transplantation. However, the biology of CB HSCs during cytokine‐mediated ex vivo expansion, such as apoptosis or expression of adhesion molecules, has not yet been elucidated. We have investigated the patterns of apoptosis and CD44 expression on human CB CD34+ cells during ex vivo expansion. CD34+ cells isolated from human CB were cultured in a stroma‐free liquid culture system with thrombopoietin (TPO), flt3‐ligand (FL), stem cell factor (SCF), and/or granulocyte‐colony stimulating factor (G‐CSF). During the culture, for up to 5 weeks, apoptosis was measured by staining with 7‐amino‐actinomycin D (7‐AAD) along with concurrent immunophenotyping of CD34 and CD44 with three‐colour flow cytometry. In the cultures with TPO, an apoptotic fraction with down‐regulated CD44 appeared from the fourth day up to the second week. G‐CSF also induced apoptosis but in a different manner; the apoptotic fraction without down‐regulation of CD44 appeared unremittingly for up to 5 weeks. FL did not induce apoptosis or down‐regulation of CD44. These findings show that apoptosis is indeed involved in the regulation of CB CD34+ cells in ex vivo expansion and the patterns of apoptosis are dependent on the type of cytokines used. The distinct patterns of apoptosis suggest different mechanisms of TPO and G‐CSF in inducing apoptosis, which still remains to be elucidated.
Septicemia, Endocarditis, and Cerebral Infarction Due to Staphylococcus aureus in a Harp seal (phoca groenlandica)

Abstract An adult, wild-collected, male harp seal (Phoca groenlandica) was transferred from a rehabilitation center to a display facility because of unilateral phthisis bulbi and decreased use of the right forelimb, which precluded its release. In quarantine, the animal demonstrated limited use of the right forelimb, which acutely progressed to complete disuse of the limb accompanied by intermittent lethargy. One month after transfer, the animal was found dead on exhibit. Necropsy showed septic arthritis of the right scapulohumeral joint, valvular endocarditis with systemic bacterial thromboembolism, and infarction of the cerebrum and myocardium. Culture of the blood and affected joint space revealed Staphylococcus aureus. Bacterial polymerase chain reaction of formalin-fixed tissues from the heart and brain were also positive for S. aureus. Staphylococcus aureus infection should be considered as an additional cause of endocarditis and embolic encephalitis in seals.