Miracle-dz/UniFAR-FTUs huggingface dataset:
Apoptosis Inhibition by the Human DEK Oncoprotein Involves Interference with p53 Functions

ABSTRACT The DEK proto-oncogene has been associated with human carcinogenesis—either as a fusion with the CAN nucleoporin protein or when transcriptionally upregulated. Mechanisms of intracellular DEK functions, however, have remained relatively unexplored. We have recently demonstrated that DEK expression is induced by the high-risk human papillomavirus (HPV) E7 protein in a manner which is dependent upon retinoblastoma protein function and have implicated DEK in the inhibition of cellular senescence. Additionally, overexpression of DEK resulted in significant life span extension of primary human keratinocytes. In order to determine whether DEK expression is required for cellular proliferation and/or survival, we monitored cellular responses to the knockdown of DEK in cancer and primary cells. The results indicate that DEK expression protects both HPV-positive cancer and primary human cells from apoptotic cell death. Cell death in response to DEK depletion was accompanied by increased protein stability and transcriptional activity of the p53 tumor suppressor and consequent upregulation of known p53 target genes such as p21CIP and Bax. Consistent with a possible role for p53 in DEK-mediated cell death inhibition, the p53-negative human osteosarcoma cell line SAOS-2 was resistant to the knockdown of DEK. Finally, expression of a dominant negative p53 miniprotein inhibited DEK RNA interference-induced p53 transcriptional induction, as well as cell death, thus directly implicating p53 activation in the observed apoptotic phenotype. These findings suggest a novel role for DEK in cellular survival, involving the destabilization of p53 in a manner which is likely to contribute to human carcinogenesis.
Peripheral blood sCD3− CD4+ T cells: a useful diagnostic tool in angioimmunoblastic T cell lymphoma

Angioimmunoblastic T cell lymphoma (AITL) belongs to the subgroup of mature T cell lymphomas according to the World Health Organization and is one of the common T cell lymphomas in Western countries. Particularly in cases in which histological confirmation cannot be easily achieved, immunophenotyping of peripheral blood can give important information for the differential diagnosis of AITL. sCD3− CD4+ T cells are a typical feature of AILT in flow cytometry of peripheral blood. In this retrospective study, the diagnostic value of flow cytometry for the diagnosis ‘AITL’ was assessed by comparing the frequency of sCD3− CD4+ T cells in leukemic AITL patients and in patients with other leukemic CD4+ T cell lymphomas. Immunophenotyping of peripheral blood by flow cytometry was performed in a lymphocyte gate using fluorochrome‐labelled antibodies against CD3, CD2, CD4, CD5, CD7, CD8, CD10, CD14, CD16, CD19, CD56, CD57 and T cell receptor. In 17/17 leukemic AITL patients, a small but distinct population of sCD3− CD4+ T cells was detected (mean percentage of sCD3− CD4+ T cells in the lymphocyte gate: 11.9 ± 15.4%, range 0.1–51.8%). In contrast, sCD3− CD4+ T cells were found in only 1/40 patients with other leukemic CD4+ T cell lymphomas (one patient with mycosis fungoides). sCD3− CD4+ T cells have a high positive predictive value (94%) for the diagnosis ‘AITL’. Flow cytometry is particularly useful in the differential diagnosis of AITL, even if the aberrant T cell population has a very low frequency. Further biological characterization of this subfraction of lymphoma cells is warranted. Copyright © 2013 John Wiley & Sons, Ltd.
Superintendents' leadership in Alberta schools: Influencing school improvement?

This study assessed the link between superintendents' leadership and its influence on school improvement practice in small and medium sized Alberta school jurisdictions (student populations fewer than 30 000). Principals' perceptions were used to determine if the superintendent’s influence was the same as internal factors such as school council, school culture, school goals, and student needs. In addition this study determined if the superintendents' influence was the same as all of the internal factors when combined. The study used Alberta Education reports to identify school restructuring components that were key to the school reform movement in Alberta in 1994. A review of the literature revealed that Leithwood (1995) had identified the main internal influencing factors in school improvement, thereby providing a basis for the comparison. Descriptive statistics were used to track the data and to compare the respective influence of all factors. These comparisons were validated using a chi-squared calculation on each influencing factor. The study found that the superintendent's influence in Alberta schools exceeded each of the internal factors in its impact on school improvement practice in a restructured setting. The study also determined that the superintendent's influence was at least equal to that of all the internal factors combined. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.
New coumarin from the roots of Prangos pabularia

Abstract The new coumarin 1, yuganin A (7-methoxy-8-((1S,2S)-1,2,3-trihydroxy-3-methylbutyl)-2H-chromen-2-one) along with nine known coumarins, heraclenol 3′-O-β-D-glucopyranoside (2), oxypeucedanin hydrate 3′-O-β-D-glucopyranoside (3), heraclenol (4), oxypeucedanin hydrate (5), osthole (6), oxypeucedanin (7), heraclenin (8), isoimperatorin (9), imperatorin (10) and the disaccharide sucrose (11), have been isolated from the roots of Prangos pabularia, and the structures of these isolated compounds were elucidated by spectroscopic means, especially, UV, HR-ESIMS, and 1D and 2D NMR spectroscopy. Furthermore, the anti-melanogenic effect of yuganin A and its inhibitory effect on B16 cells were evaluated. Yuganin A may be useful in the treatment of hyperpigmentation and as a skin-whitening agent in the cosmetics industry.
Chronic psychophysiological insomnia: hyperarousal and/or inhibition deficits? An ERPs investigation.

STUDY OBJECTIVES Chronic primary insomnia has been hypothesized to result from conditioned arousal or the inability to initiate normal sleep processes. The event-related potentials (ERPs) N1, P2, and N350 are useful indexes of arousal. The objective is to compare these ERPs in primary chronic psychophysiological insomniacs (INS) and good sleepers (GS) during multiple recordings. PARTICIPANTS Participants were 15 INS (mean age = 46 years, SD = 7.5) and 16 GS (mean age = 37 years, SD = 10.1). METHODS AND PROCEDURE Following a multistep clinical evaluation, INS and GS participants underwent 4 consecutive nights of PSG recordings (N1 to N4). ERPs were recorded on the 3rd and 4th nights in the sleep laboratory (N3 and N4). ERPs recordings were made during wake on both nights (in the evening and upon awakening), with the addition of sleep-onset recordings on N4. Auditory stimuli consisted of "standard" and "deviant" tones. STATISTICAL ANALYSIS Repeated measures ANOVAs were computed for each ERP for each recording for each type of stimulus. RESULTS The amplitude of P2 and N350 was greater for the deviant than for the standard stimulus in both groups. The amplitude of N1 was larger in INS than GS in the morning and the evening. While the amplitude of N350 was larger in GS than in INS at sleep onset, the amplitude of P2 was greater in INS than in GS at that time. CONCLUSION Signs of greater cortical arousal in psychophysiological insomnia individuals are observed, especially upon awakening in the morning. However, at sleep onset, difficulties from disengaging from wake processes and some inability at initiating normal sleep processes appear also present in individuals with insomnia compared to good sleepers.
The use of complimentary assays to evaluate the enrichment of human sperm quality in asthenoteratozoospermic and teratozoospermic samples processed with Annexin‐V magnetic activated cell sorting

Sperm chromatin integrity may affect the outcomes of assisted reproductive technology (ART). Developing a clinically reliable strategy to enrich sperm samples with high chromatin quality spermatozoa prior to sperm banking or use in ART would thus be advantageous. The objectives of this study were to: (i) assess the sperm chromatin quality in men with different categories of semen parameters; and (ii) evaluate the extents of Annexin‐V magnetic‐activated cell sorting (MACS) technology coupled with differential density gradient centrifugation (DGC) in improving sperm chromatin quality. Three categories of men from couples attending a university‐based fertility clinic were recruited based on their semen parameters: normozoospermic (n = 13), asthenoteratozoospermic (n = 17) and teratozoospermic (n = 12). For each patient, spermatozoa in semen samples were processed first by DGC to enrich the motility and further by MACS to remove spermatozoa showing apoptotic features. The yield and enrichment of sperm quality was evaluated at each step with conventional semen parameters in conjunction with a combination of five complementary assays, to assess sperm maturity, chromatin structure, compaction and DNA integrity (Hyaluronic Binding Assay, SCSA, chromomycine A3 staining and TUNEL and COMET assays). Our results demonstrated that, compared with normozoospermic samples, raw asthenoteratozoospermic and teratozoospermic samples had a higher proportion of spermatozoa containing DNA breaks, but only asthenoteratozoospermic exhibited altered chromatin structure and decreased binding to hyaluronic acid. Interestingly, the DGC appeared to select for more mature spermatozoa with high DNA compaction. More importantly, in all categories of semen samples, Annexin‐V MACS allows enrichment of spermatozoa with good chromatin quality as measured by the TUNEL and SCSA. Because effective treatment modalities to improve sperm DNA damage are limited, our results suggest a potential clinical value of MACS as a mean to enhance sperm quality that may improve assisted reproductive outcomes.
Is the incremental transparency necessary?

ABSTRACT This paper examines how liquidity, price discovery and the causality between them are affected by incremental transparency on the Taiwan Stock Exchange (TSEC). We find that it has small effects on market quality, with the impact being focused on small- or medium-sized firms. We also find that the causality between liquidity and price discovery is weakened after the event. This study is a supplement to the existing literature and our results imply that if the market is already considerably transparent, then the effects of further opening up the limit-order book may be limited.
Re‐induction of complete remission with a new synthetic retinoid, Am‐80, for relapse of acute promyelocytic leukaemia previously treated with all‐trans retinoic acid

Two patients with relapsed acute promyelocytic leukaemia previously treated with all‐trans retinoic acid (ATRA), were treated with a new synthetic retinoid, Am‐80. In both patients pancytopenia gradually resolved without an increase in leukaemic cells, and differentiation of leukaemic cells was observed morphologically in bone marrow. Without the use of anti‐leukaemic agents, both cases achieved complete remission (CR) on days 52 and 38 of treatment, respectively. On the day of CR, PML gene rearrangement and the t(15;17) translocation disappeared, though PML‐RAR α chimaeric messenger RNA was still detected by reverse transcriptase polymerase chain reaction. Both patients then received conventional chemotherapy for consolidation of CR. These clinical experiences suggest that Am‐80 may be an active agent for APL patients who have relapsed from ATRA‐induced remission.
Interconnected microgrids via back-to-back converters for dynamic frequency support

This paper presents the back-to-back interconnection of two autonomous microgrids. A back-to-back converter topology, referred to as an interchange converter, is used to provide dynamic frequency support for each autonomous microgrid. The proposed interchange converter facilitates active power exchange between the two microgrids when one cluster does not have sufficient generation capacity to supply its local load demand. During an overloading in the first microgrid, the output power of the energy resources within the other microgrid increases to supply the loads in the first microgrid. Hence, the frequency in both microgrids is kept within the acceptable range. Several study cases are simulated in DIgSILENT Power-Factory software to investigate and verify the successful dynamic frequency support by the help of the interchange converter.
Unit Commitment Model and Economic Dispatch Model Based on Real Time Pricing for Large-Scale Wind Power Accommodation

A stochastic unit commitment model and deterministic unit commitment model based on real time pricing(RTP) for large-scale wind power accommodation are respectively proposed in this paper considering the positive effect of demand response(DR) and the characteristic of the uncertainty scheduling strategy.Economic dispatch model is established to evaluate the performance of different unit commitment strategies.In addition,the influence of transmission constraint is discussed in this paper.Case study based on PJM-5 bus indicates that DR can be flexibly deployed to adapt to the change of wind power output which could cope with wind power uncertainty and it will increase the economic efficiency,robustness and wind power utilization rate.Specifically,DR resource can be co-optimized with generators operation to satisfy the requirement of all scenarios in stochastic unit commitment,while DR can alleviate the issues that less uncertainty is covered and reserve decision without considering the topology of power grid which may lead to some reserve cannot be released in deterministic model.Furthermore,deterministic model requires DR with more flexibility and higher capacity comparing with stochastic one.
MiR-20a-5p promotes radio-resistance by targeting NPAS2 in nasopharyngeal cancer cells

MicroRNAs (miRNAs) are key players of gene expression involved in diverse biological processes including the cancer radio-resistance, which hinders the effective cancer therapy. Here we found that the miR-20a-5p level is significantly up-regulated in radio-resistant nasopharyngeal cancer (NPC) cells via an RNA-seq and miR-omic analysis. Moreover, we identified that the neuronal PAS domain protein 2 (NPAS2) gene is one of the targets of miR-20a-5p. The involvement of miR-20a-5p and NPAS2 with NPC radio-resistance was further validated by either down- or up-regulation of their levels in NPC cell lines. Taken together, these results not only reveal novel insights into the NPC radio-resistance, but also provide hints for an effective therapeutic strategy to fight against NPC radio-resistance.
An efficient tunable selective points-to analysis for large codebases

Points-to analysis is a fundamental static program analysis technique for tools including compilers and bug-checkers. Although object-based context sensitivity is known to improve precision of points-to analysis, scaling it for large Java codebases remains a challenge. In this work, we develop a tunable, client-independent, object-sensitive points-to analysis framework where heap cloning is applied selectively. This approach is aimed at large codebases where standard analysis is typically expensive. Our design includes a pre-analysis that determines program points that contribute to the cost of an object-sensitive points-to analysis. A subsequent analysis then determines the context depth for each allocation site. While our framework can run standalone, it is also possible to tune it – the user of the framework can use the knowledge of the codebase being analysed to influence the selection of expensive program points as well as the process to differentiate the required context-depth. Overall, the approach determines where the cloning is beneficial and where the cloning is unlikely to be beneficial. We have implemented our approach using Soufflé (a Datalog compiler) and an extension of the DOOP framework. Our experiments on large programs, including OpenJDK, show that our technique is efficient and precise. For the OpenJDK, our analysis reduces 27% of runtime and 18% of memory usage in comparison with 2O1H points-to analysis for a negligible loss of precision, while for Jython from the DaCapo benchmark suite, the same analysis reduces 91% of runtime for no loss of precision.
Methodological considerations for studying social processes.

AIM To discuss the nature of and considerations in the study of social processes. BACKGROUND Social processes include the elements of time, change and human interaction and many phenomena of interest to nurse researchers. Despite the significance of social processes for nursing practice and the labelling in many studies of phenomena as processes, there seems to be an inability to describe processes fully. DISCUSSION The paper includes a presentation of two methodological approaches for illuminating the dynamics of social processes: participant observation and prospective time-series designs. Strengths and limitations of the two paradigmatically different approaches are offered. The method an investigator chooses should be considered selectively and appropriately according to the nature of the problem, what is known about the phenomena to be studied, and the investigator's world view and theoretical perspective. The conceptualisation of process can also influence the methodological choice. IMPLICATIONS FOR RESEARCH/PRACTICE Capturing a social process in its entirety with either a qualitative or quantitative approach can be a difficult task. The focus of this paper is an initiation and expansion of the dialogue about which methods provide the best insight into social processes. This knowledge may offer opportunities for nurse researchers to design and implement interventions for individuals as they progress through life events.
Anti-Hinge Antibodies Recognize IgG Subclass– and Protease-Restricted Neoepitopes

Anti-hinge Abs (AHAs) target neoepitopes exposed after proteolytic cleavage of IgG. In this study, we explored the diversity of protease- and IgG subclass–restricted AHAs and their potential as immunological markers in healthy donors (HDs) and patients with rheumatoid arthritis (RA) or systemic lupus erythematosus (SLE). AHA reactivity against IgG-degrading enzyme of Streptococcus pyogenes (IdeS)– or pepsin-generated F(ab′)2 fragments of all four human IgG subclasses was determined. AHA reactivity against one or more out of eight F(ab′)2 targets was found in 68% (68 of 100) of HDs, 69% (68 of 99) of SLE patients, and 81% (79 of 97) of RA patients. Specific recognition of hinge epitopes was dependent on IgG subclass and protease used to create the F(ab′)2 targets, as confirmed by inhibition experiments with F(ab′)2 fragments and hinge peptides. Reactivity against IdeS-generated F(ab′)2 targets was found most frequently, whereas reactivity against pepsin-generated F(ab′)2 targets better discriminated between RA and HDs or SLE, with significantly higher AHA levels against IgG1/3/4. In contrast, AHA levels against pepsin-cleaved IgG2 were comparable. No reactivity against IdeS-generated IgG2-F(ab′)2s was detected. The most discriminatory AHA reactivity in RA was against pepsin-cleaved IgG4, with a 35% prevalence, ≥5.8-fold higher than in HDs/SLE, and significantly higher levels (p < 0.0001). Cross-reactivity for F(ab′)2s generated from different IgG subclasses was only observed for subclasses having homologous F(ab′)2 C termini (IgG1/3/4). For IgG2, two pepsin cleavage sites were identified; anti-hinge reactivity was restricted to only one of these. In conclusion, AHAs specifically recognize IgG subclass– and protease-restricted hinge neoepitopes. Their protease-restricted specificity suggests that different AHA responses developed under distinct inflammatory or infectious conditions and may be markers of, and participants in, such processes.
When the Child is Born into the Internet: Sharenting as a Growing Trend among Parents on Facebook

Parents actively share information about their children on Facebook, but little research has explored the extent of this issue. The goal of this paper is to theorize a new type of parents’ online activities concerning their children, especially the problem of sharenting, which is increasingly common in contexts where social media such as Facebook play a significant role in relationships and interactions. This paper explores what kind of baby pictures parents share on Facebook and what are the likely causes of doing it. The presented research was conducted with the use of social media ethnography among 168 Polish parents using Facebook. The findings have shown that the phenomenon of sharenting is common practice among parents.
Direct reconstructive venous surgery.

Unilateral occlusions of the pelvic veins may be corrected by a Palma operation. In the classic Palma operation the contralateral long saphenous vein is suprapubicly led to the opposite side and is anastomosed end-to-end or end-to-side with the common femoral vein of the occluded side. Just lately, spirally wall reinforced PTFE grafts showed more favourable results than Palma grafts. Circumscribed occlusions of the superficial femoral vein can be interposed by the May-Husni operation. Thereby the ipsilateral long saphenous vein is implanted end-to-side into the popliteal vein. We perform the Palma operation as well as the May-Husni operation under the protection of a temporary av-fistula. The ascending venography only permits to make a statement on the morphological operability, whereas the functional operability is indicated by means of phlebodynamometry. Phlebodynamometry and plethysmography are the best means to judge the functional result of the operation which is of importance for the patient. The results of the Palma operation can be regarded as very satisfactory, provided that the morphological and functional indication for the operation was correct. The use of the endothelium protective operative technique by Robert Mays as well as the application of a temporary av-fistula yield a success that is above 80 p.c. As against that, the May-Husni operation achieves less than 50 p.c. success, even if a temporary av-fistula is established in the area of the ancle. Less favourable are the results obtained by direct restorative interventions on the subclavian vein, so that these operations should only be indicated in exceptional cases.
Museum collection management on-demand

In this case study, we trace the rationale, development and operation of the Integrated Museum Collection Management System as a software-as-a-service solution for public as well as private museums in Singapore. This on-demand service may serve as a model for other public services particularly in the context of business process re-engineering and standards adoption. It comprises a traditional client-server solution for internal users and a web frontend for public search. From the operation perspective, the service has posed challenges in delivery and support. Lastly, standards and technology adoption issues have to be addressed.