Miracle-dz/UniFAR-FTUs huggingface dataset:
Transient structures in rupturing thin films: Marangoni-induced symmetry-breaking pattern formation in viscous fluids

A transient pattern formation on the top of a soap bubble induced by surface tension gradients is examined via asymptotics. In the minutes immediately preceding the rupture of a soap bubble, distinctive and repeatable patterns can be observed. These quasistable transient structures are associated with the instabilities of the complex Marangoni flows on the curved thin film in the presence of a surfactant solution. Here, we report a generalized Cahn-Hilliard-Swift-Hohenberg model derived using asymptotic theory that describes the quasielastic wrinkling pattern formation and the consequent coarsening dynamics in a curved surfactant-laden thin film. By testing the theory against experiments on soap bubbles, we find quantitative agreement with the analytical predictions of the nucleation and the early coarsening phases associated with the patterns. Our findings provide fundamental physical understanding that can be used to (de-)stabilize thin films in the presence of surfactants and have important implications for both natural and industrial contexts, such as the production of thin coating films, foams, emulsions, and sprays.
Protein kinase Cα is differentially activated during neonatal and adult erythropoiesis and favors expression of a reporter gene under the control of the Aγ globin‐promoter in cellular models of hemoglobin switching

PKCα was found to be expressed (mRNA and protein) throughout the in vitro maturation of primary human erythroblasts but its activity (phosphorylation levels and nuclear localization) was consistently higher in cells derived from human neonatal rather than adult blood. Since the γ/γ + β globin expression ratio represented the major difference between neonatal and adult erythroblasts (58 ± 12 vs. 7 ± 3, respectively), we tested the hypothesis that PKCα might affect γ‐globin expression by measuring the levels of Aγ‐ or β‐promoter‐driven reporter activity in erythroid cells stably (GM979) or transiently (K562, primary adult and neonatal erythroblasts) transfected with a dual µLCRβprRlucAγprFluc reporter in the presence of transient expression of either the constitutively active (sPKCα) or catalytically inactive (iPKCα) PKCα. As further control, GM979 cells were incubated with the PKC inhibitor rottlerin (30 µM). In all the cells analyzed, sPKCα significantly increased (by two‐ to sixfold) the levels of luciferase activity driven by the Aγ‐promoter and the Aγ‐F/(Aγ‐F + 2β‐R) expression ratio. In GM979 cells, rottlerin inhibited (by 50%) the Aγ‐driven luciferase activity and the Aγ‐F/(Aγ‐F + 2β‐R) expression ratio. These results suggest that different PKC isoforms may exert ontogenetic‐specific functions in erythropoiesis and that modulation of PKCα might affect the activity of Aγ‐promoter‐driven reporters. J. Cell. Biochem. 101: 411–424, 2007. © 2007 Wiley‐Liss, Inc.
Reliable and Rapid Identification of Listeria monocytogenes and Listeria Species by Artificial Neural Network-Based Fourier Transform Infrared Spectroscopy

ABSTRACT Differentiation of the species within the genus Listeria is important for the food industry but only a few reliable methods are available so far. While a number of studies have used Fourier transform infrared (FTIR) spectroscopy to identify bacteria, the extraction of complex pattern information from the infrared spectra remains difficult. Here, we apply artificial neural network technology (ANN), which is an advanced multivariate data-processing method of pattern analysis, to identify Listeria infrared spectra at the species level. A hierarchical classification system based on ANN analysis for Listeria FTIR spectra was created, based on a comprehensive reference spectral database including 243 well-defined reference strains of Listeria monocytogenes, L. innocua, L. ivanovii, L. seeligeri, and L. welshimeri. In parallel, a univariate FTIR identification model was developed. To evaluate the potentials of these models, a set of 277 isolates of diverse geographical origins, but not included in the reference database, were assembled and used as an independent external validation for species discrimination. Univariate FTIR analysis allowed the correct identification of 85.2% of all strains and of 93% of the L. monocytogenes strains. ANN-based analysis enhanced differentiation success to 96% for all Listeria species, including a success rate of 99.2% for correct L. monocytogenes identification. The identity of the 277-strain test set was also determined with the standard phenotypical API Listeria system. This kit was able to identify 88% of the test isolates and 93% of L. monocytogenes strains. These results demonstrate the high reliability and strong potential of ANN-based FTIR spectrum analysis for identification of the five Listeria species under investigation. Starting from a pure culture, this technique allows the cost-efficient and rapid identification of Listeria species within 25 h and is suitable for use in a routine food microbiological laboratory.
Biochemical demonstration of complex formation of histone pre‐mRNA with U7 small nuclear ribonucleoprotein and hairpin binding factors.

Histone RNA 3′ end formation occurs through a specific cleavage reaction that requires, among other things, base‐pairing interactions between a conserved spacer element in the pre‐mRNA and the minor U7 snRNA present as U7 snRNP. An oligonucleotide complementary to the first 16 nucleotides of U7 RNA can be used to characterize U7 snRNPs from nuclear extracts by native gel electrophoresis. Using similar native gel techniques, we present direct biochemical evidence for a stable association between histone pre‐mRNA and U7 snRNPs. Other complexes formed in the nuclear extract are dependent on the 5′ cap structure and on the conserved hairpin element of histone pre‐mRNA, respectively. However, in contrast to the U7‐specific complex, their formation is not required for processing. Comparison of several authentic and mutant histone pre‐mRNAs with different spacer sequences demonstrates that the formation and stability of the U7‐specific complex closely follows the predicted stability of the potential RNA‐RNA hybrid. However, this does not exclude a stabilization of the complex by U7 snRNP structural proteins.
Reproductive character displacement of female, but not male song discrimination in an avian hybrid zone

Divergence of male sexual signals and female preferences for those signals often maintains reproductive boundaries between closely related, co‐occurring species. However, contrasting sources of selection, such as interspecific competition, can lead to weak divergence or even convergence of sexual signals in sympatry. When signals converge, assortative mating can be maintained if the mating preferences of females diverge in sympatry (reproductive character displacement; RCD), but there are few explicit examples. Pied flycatchers (Ficedula hypoleuca) are sympatric with collared flycatchers (F. albicollis) on the Baltic island of Öland, where males from both species compete over nestboxes, their songs converge, and the two species occasionally hybridize. We compare song discrimination of male and female pied flycatchers on Öland and in an allopatric population on the Swedish mainland. Using field choice trials, we show that male pied flycatchers respond similarly to the songs of both species in sympatry and allopatry, while female pied flycatchers express stronger discrimination against heterospecific songs in sympatry than in allopatry. These results are consistent with RCD of song discrimination of female pied flycatchers where they co‐occur with collared flycatchers, which should maintain species assortative mating despite convergence of male sexual signals.
Design and Evaluation of a Simulation for Pediatric Dentistry in Virtual Worlds

Background Three-dimensional virtual worlds are becoming very popular among educators in the medical field. Virtual clinics and patients are already used for case study and role play in both undergraduate and continuing education levels. Dental education can also take advantage of the virtual world’s pedagogical features in order to give students the opportunity to interact with virtual patients (VPs) and practice in treatment planning. Objective The objective of this study was to design and evaluate a virtual patient as a supplemental teaching tool for pediatric dentistry. Methods A child VP, called Erietta, was created by utilizing the programming and building tools that online virtual worlds offer. The case is about an eight-year old girl visiting the dentist with her mother for the first time. Communication techniques such as Tell-Show-Do and parents’ interference management were the basic elements of the educational scenario on which the VP was based. An evaluation of the simulation was made by 103 dental students in their fourth year of study. Two groups were formed: an experimental group which was exposed to the simulation (n=52) and a control group which did not receive the simulation (n=51). At the end, both groups were asked to complete a knowledge questionnaire and the results were compared. Results A statistically significant difference between the two groups was found by applying a t test for independent samples (P<.001), showing a positive learning effect from the VP. The majority of the participants evaluated the aspects of the simulation very positively while 69% (36/52) of the simulation group expressed their preference for using this module as an additional teaching tool. Conclusions This study demonstrated that a pediatric dentistry VP built in a virtual world offers significant learning potential when used as a supplement to the traditional teaching techniques.
Semiparametric Inference for Proportional Mean Past Life Model

Abstract The mean past lifetime provides the expected time elapsed since the failure of a subject given that he/she has failed before the time of observation. In this paper, we propose the proportional mean past lifetime model to study the association between the mean past lifetime function and potential regression covariates. In the presence of left censoring, martingale estimating equations are developed to estimate the model parameters, and the asymptotic properties of the resulting estimators are studied. To assess the adequacy of the model, a goodness of fit test is also investigated. The proposed method is evaluated via simulation studies and further applied to a data set.
Binding of PTEN to Specific PDZ Domains Contributes to PTEN Protein Stability and Phosphorylation by Microtubule-associated Serine/Threonine Kinases*

The tumor suppressor phosphatase PTEN is a key regulator of cell growth and apoptosis that interacts with PDZ domains from regulatory proteins, including MAGI-1/2/3, hDlg, and MAST205. Here we identified novel PTEN-binding PDZ domains within the MAST205-related proteins, syntrophin-associated serine/threonine kinase and MAST3, characterized the regions of PTEN involved in its interaction with distinctive PDZ domains, and analyzed the functional consequences on PTEN of PDZ domain binding. Using a panel of PTEN mutations, as well as PTEN chimeras containing distinct domains of the related protein TPTE, we found that the PTP and C2 domains of PTEN do not affect PDZ domain binding and that the C-terminal tail of PTEN (residues 350–403) provides selectivity to recognize specific PDZ domains from MAGI-2, hDlg, and MAST205. Binding of PTEN to the PDZ-2 domain from MAGI-2 increased PTEN protein stability. Furthermore, binding of PTEN to the PDZ domains from microtubule-associated serine/threonine kinases facilitated PTEN phosphorylation at its C terminus by these kinases. Our results suggest an important role for the C-terminal region of PTEN in the selective association with scaffolding and/or regulatory molecules and provide evidence that PDZ domain binding stabilizes PTEN and targets this tumor suppressor for phosphorylation by microtubule-associated serine/threonine kinases.
The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus

Background: A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 ("Cornu Ammonis" area 1) neurons. This study aimed to verify reactive gliosis and neuronal damage after injection of the vector into the rat hippocampus. Methods: HEK 293T cells were transfected with transfer (fck-Jaws-GFP-ER2), envelope (FUG-B2), and packaging (pMDLg/pRRE, pRSV-Rev) plasmids, and the vector was injected into CA1 of the rat hippocampus. After one week, transduction efficiency, and the number of neuronal and astroglial cells were determined in CA1 and CA3 by double staining of the brain slices. Results: Hippocampal cells were successfully transfected as 92.7% of CA1 and 95.8% of CA3 neuronal cells expressed GFP. The frequency of neuronal and astroglial cells in CA1 and CA3 of the vector-injected rats remained unchanged compared to those in the control and the saline-injected rats. Furthermore, no morphological change was found in hippocampal astrocytes and neuronal cells. Conclusion: The HIV-1-based LV pseudotyped by FUG-B2 is safe and does not cause neuroinflammation and neuronal loss once directly delivered into the rat hippocampus.
Specific Diagnosis of Exogenous Bronchial Asthma in Adults

Twenty‐two adults with extrinsic bronchial asthma were examined with 26 different allergens from five groups: house dust + mite (30 BP), animal dandruff (30 BP), pollen (12 BP), mould fungi (11 BP), and feather + cotton (10 BP). The same allergen batch was used for each allergen extract in all analyses.
Cost efficient frequency hopping radar waveform for range and Doppler estimation

A simple and cost efficient frequency hopping radar waveform which has been developed to be robust against mutual interference is presented. The waveform is based on frequency hopping on the one hand and Linear Frequency Modulation Shift Keying (LFMSK) on the other hand. The signal model as well as the radar signal processing are described in detail, focusing on the efficient estimation of range and Doppler of multiple targets in parallel. Finally, the probability of interference is estimated and techniques for interference mitigation are presented showing a good suppression of mutual interference of the same type of radar.
Regional Distribution of Blood Flow during Arterial Hypertension Produced by Lesions of the Nucleus Tractus Solitarii in Rats

Changes in the fractional distribution of cardiac output (FF), organ blood flow, and regional vascular resistance were measured by the isotope dilution technique of Sapirstein using86Rb as indicator in unanesthetized rats during acute arterial hypertension produced by bilateral lesions of the nucleus tractus solitarii (NTS). After NTS lesions, the FF was significantly reduced in skin, muscle, and colon, increased in ventricular myocardium, spleen, and adrenal glands, and was unchanged elsewhere. Because of a marked reduction in cardiac output (CO) during hypertension, the absolute organ blood flow (FF x CO) was reduced in lesioned rats to 30-40% of control in skin, muscle, and colon and between 60% and 75% of control in most of the remainder of the gastrointestinal tract and renal cortex; it was unchanged in myocardium and endocrine glands. Resistance was substantially increased (4- to 6-fold) in skin, muscle and colon but was only moderately increased (1.5- to 2.5-fold) in the remaining organs. The results indicate that, while NTS lesions will increase resistance in most vascular beds, the response is unequally distributed, influencing skin, muscle, and colon disproportionately to other tissues. Because of an interaction between a reduction in CO and little autoregulation, blood flow is reduced primarily in skin, muscle, and colon. The pattern of redistribution of CO was consistent with the interpretation that NTS hypertension results from interrupting baroreceptor reflexes centrally. The pattern of redistribution of blood flow in rats with NTS lesions differs from that produced by deoxycorticosterone acetate-salt and renal ischemia.
Wavelet denoising in voxel-based parametric estimation of small animal PET images: a systematic evaluation of spatial constraints and noise reduction algorithms

Voxel-based estimation of PET images, generally referred to as parametric imaging, can provide invaluable information about the heterogeneity of an imaging agent in a given tissue. Due to high level of noise in dynamic images, however, the estimated parametric image is often noisy and unreliable. Several approaches have been developed to address this challenge, including spatial noise reduction techniques, cluster analysis and spatial constrained weighted nonlinear least-square (SCWNLS) methods. In this study, we develop and test several noise reduction techniques combined with SCWNLS using simulated dynamic PET images. Both spatial smoothing filters and wavelet-based noise reduction techniques are investigated. In addition, 12 different parametric imaging methods are compared using simulated data. With the combination of noise reduction techniques and SCWNLS methods, more accurate parameter estimation can be achieved than with either of the two techniques alone. A less than 10% relative root-mean-square error is achieved with the combined approach in the simulation study. The wavelet denoising based approach is less sensitive to noise and provides more accurate parameter estimation at higher noise levels. Further evaluation of the proposed methods is performed using actual small animal PET datasets. We expect that the proposed method would be useful for cardiac, neurological and oncologic applications.
Aiming at a Curative Strategy for Follicular Lymphoma

Follicular lymphoma is often managed as an incurable disease. However, a substantial and growing fraction of patients are achieving long‐term disease‐free survival from aggressive treatment approaches. The application of novel therapeutic tools, including monoclonal antibodies, radioimmunotherapy, and vaccines, as well as new and more active chemotherapeutic agents, is producing complete responses in the majority of treated patients, with a 2‐fold increase in disease‐ and progression‐free survival in randomized trials. For some of these treatment approaches, follow up has not yet been long enough to determine a median response duration, but it certainly exceeds the “2 to 3 years” that is routinely stated as dogma to patients with this illness. Furthermore, some patients remain in complete remission beyond a decade from their initial treatment, implying that the assumption of inevitable relapse also must be challenged. One clear fact is that no patients will ever be cured by adopting a palliative treatment approach. The assumption that patients with follicular lymphoma are incurable is certain to be a self‐fulfilling prophecy. Here the author summarizes the large and growing body of knowledge that suggests an expectant approach to management is not appropriate for all patients.
Red‐shifted fluorescent proteins monitor enzymatic activity in live HT‐1080 cells with fluorescence lifetime imaging microscopy (FLIM)

Membrane type 1 matrix metalloproteinase (MT1‐MMP) is a membrane‐tethered collagenase primarily involved in the mechanical destruction of extracellular matrix proteins. MT1‐MMP has also been shown to be upregulated in several types of cancers. Many coordinated functions of MT1‐MMP during migration and invasion remain to be determined. In this paper, live cells from the invasive cell line HT‐1080 were imaged using an intracellular Förster resonance energy transfer‐based biosensor specific for MT1‐MMP; a substrate specific for MT1‐MMP was hybridized with the mOrange2 and mCherry fluorescent proteins to form the Förster resonance energy transfer‐based sensor. The configuration of the biosensor was determined with fluorescence lifetime‐resolved imaging microscopy using both a polar plot‐based analysis and a rapid data acquisition modality of fluorescence lifetime‐resolved imaging microscopy known as phase suppression. Both configurations of the biosensor (with or without cleavage by MT1‐MMP) were clearly resolvable in the same cell. Changes in the configuration of the MT1‐MMP biosensor were observed primarily along the edge of the cell following the removal of the MMP inhibitor GM6001. The intensities highlighted by phase suppression correlated well with the fractional intensities derived from the polar plot.
Bilaterally Dislocated Hips Treated With the Pavlik Harness Are not at a Higher Risk for Failure

Background The purpose of this study is to evaluate the efficacy of the Pavlik harness treatment in patients with bilaterally dislocated hips, and to compare them with cases with unilaterally dislocated hips. Methods Twenty-nine patients (58 hips) were identified who presented with bilateral dislocated hips with no earlier treatment and received dynamic ultrasound examination. The comparison group consisted of 38 patients (38 hips) with unilateral hip dislocation treated with the same protocol. Successful treatment was defined as relocation/reduction of the hips within 3 weeks of Pavlik harness application. Results Seventeen of 29 patients (59%) in the bilateral group (7 bilateral, 10 unilateral) and 22 of 38 patients (58%) in the unilateral group failed the harness treatment. There was no difference in the duration of harness wear for successful patients regardless of presentation. Conclusions Patients presenting with bilaterally dislocated hips treated with the Pavlik harness are at no greater risk for failure than patients presenting with unilateral hip dislocation. Level of Evidence Level 3.
Investment and Firm Value Under High Economic Uncertainty: The Beneficial Effect of Overconfident CEOs

In this paper we investigate whether managerial overconfidence benefits shareholders when economic uncertainty is high. Consistent with managerial overconfidence mitigating the underinvestment problems exacerbated by high economic uncertainty, we find that during periods of import tariff cuts and the global financial crisis, investment and firm value are higher for firms managed by overconfident CEOs than for those managed by non-overconfident CEOs. Moreover, overconfident firms’ M&A announcements are associated with more positive abnormal returns when market uncertainty as measured by the CBOE Volatility Index is higher, and overconfident firms are more likely to undertake value-increasing M&A deals.