Miracle-dz/UniFAR-FTUs huggingface dataset:
Direct Effects of Leptin on Mouse Reproductive Function: Regulation of Follicular, Oocyte, and Embryo Development

Abstract Because body condition can affect reproduction, research has focused on the role of leptin, a body condition signal, in regulation of reproductive function. Objectives of this study were to determine if leptin supplementation directly affects 1) ovarian follicle growth and function, 2) oocyte maturation, or 3) preimplantation embryo development. Follicles cultured in the presence of recombinant mouse leptin resulted in a significant decrease in rate of follicle, but not oocyte, growth in a dose-dependent manner, with higher doses of leptin inhibiting growth. Leptin was also found to significantly increase stimulated progesterone, estradiol, and testosterone production/secretion by cultured follicles in a dose-dependent manner, with higher concentrations of leptin significantly increasing steroidogenesis. Culture of fully grown cumulus-enclosed germinal vesicle-intact (GV) mouse oocytes in the presence of increasing concentrations of leptin (0, 12.5, 25, 50, 100 ng/ml) had no effect on germinal vesicle breakdown (GVBD) or development to metaphase II (MII). Similarly, fully grown denuded oocytes showed no difference in GVBD at any concentration of leptin. However, maturation of denuded oocytes with 100 ng/ml leptin resulted in significantly reduced development to MII compared with oocytes matured with 0 or 12.5 ng/ml leptin. Culture of one-cell mouse embryos in increasing concentrations of leptin had no effect on cleavage or blastomere degeneration at 24 h of culture. Exposure of embryos for the first 96 h of development to increasing concentrations of leptin did not significantly affect total or expanded blastocyst development or hatching of blastocysts from zona pellucida. These results indicate leptin directly enhances insulin and gonadotropin-stimulated ovarian steroidogenesis, compromises denuded oocyte maturation, yet has no direct effect on preimplantation embryo development.
Effect of peroxisome proliferator‐activated receptors‐γ and co‐activator‐1α genetic polymorphisms on plasma adiponectin levels and susceptibility of non‐alcoholic fatty liver disease in Chinese people

Background/Aims: Peroxisome proliferator‐activated receptors‐γ (PPAR‐γ) and its co‐activator‐1α (PGC‐1α) are involved in the regulation of lipid and glucose metabolisms. This study aimed to investigate the genetic polymorphisms of PPAR‐γ and PGC‐1α in Chinese people and their influence on plasma adiponectin levels and non‐alcoholic fatty liver disease (NAFLD) susceptibility.
Chromosomal organization of four classes of repetitive DNA sequences in killifish Orestias ascotanensis Parenti, 1984 (Cyprinodontiformes, Cyprinodontidae)

Abstract Orestias Valenciennes, 1839 is a genus of freshwater fish endemic to the South American Altiplano. Cytogenetic studies of these species have focused on conventional karyotyping. The aim of this study was to use classical and molecular cytogenetic methods to identify the constitutive heterochromatin distribution and chromosome organization of four classes of repetitive DNA sequences (histone H3 DNA, U2 snRNA, 18S rDNA and 5S rDNA) in the chromosomes of O. ascotanensis Parenti, 1984, an endemic species restricted to the Salar de Ascotán in the Chilean Altiplano. All individuals analyzed had a diploid number of 48 chromosomes. C-banding identified constitutive heterochromatin mainly in the pericentromeric region of most chromosomes, especially a GC-rich heterochromatic block of the short arm of pair 3. FISH assay with an 18S probe confirmed the location of the NOR in pair 3 and revealed that the minor rDNA cluster occurs interstitially on the long arm of pair 2. Dual FISH identified a single block of U2 snDNA sequences in the pericentromeric regions of a subtelocentric chromosome pair, while histone H3 sites were observed as small signals scattered in throughout the all chromosomes. This work represents the first effort to document the physical organization of the repetitive fraction of the Orestias genome. These data will improve our understanding of the chromosomal evolution of a genus facing serious conservation problems.
The NS4A Protein of Hepatitis C Virus Promotes RNA-Coupled ATP Hydrolysis by the NS3 Helicase

ABSTRACT Nonstructural protein 3 (NS3) is an essential replicative component of the hepatitis C virus (HCV) and a member of the DExH/D-box family of proteins. The C-terminal region of NS3 (NS3hel) exhibits RNA-stimulated NTPase and helicase activity, while the N-terminal serine protease domain of NS3 enhances RNA binding and unwinding by NS3hel. The nonstructural protein 4A (NS4A) binds to the NS3 protease domain and serves as an obligate cofactor for NS3 serine protease activity. Given its role in stimulating protease activity, we sought to determine whether NS4A also influences the activity of NS3hel. Here we show that NS4A enhances the ability of NS3hel to bind RNA in the presence of ATP, thereby acting as a cofactor for helicase activity. This effect is mediated by amino acids in the C-terminal acidic domain of NS4A. When these residues are mutated, one observes drastic reductions in ATP-coupled RNA binding and duplex unwinding by NS3. These same mutations are lethal in HCV replicons, thereby establishing in vitro and in vivo that NS4A plays an important role in the helicase mechanism of NS3 and its function in replication.
Contribution à l'analyse et à la recherche d'information en texte intégral : application de la transformée en ondelettes pour la recherche et l'analyse de textes

L’objet des systemes de recherche d’informations est de faciliter l’acces a un ensemble de documents, afin de permettre a l’utilisateur de retrouver ceux qui sont pertinents, c'est-a-dire ceux dont le contenu correspond le mieux a son besoin en information. La qualite des resultats de la recherche se mesure en comparant les reponses du systeme avec les reponses ideales que l'utilisateur espere recevoir. Plus les reponses du systeme correspondent a celles que l'utilisateur espere, plus le systeme est juge performant. Les premiers systemes permettaient d’effectuer des recherches booleennes, c’est a dire, des recherches ou seule la presence ou l’absence d’un terme de la requete dans un texte permet de le selectionner. Il a fallu attendre la fin des annees 60, pour que l’on applique le modele vectoriel aux problematiques de la recherche d’information. Dans ces deux modeles, seule la presence, l’absence, ou la frequence des mots dans le texte est porteuse d’information. D’autres systemes de recherche d’information adoptent cette approche dans la modelisation des donnees textuelles et dans le calcul de la similarite entre documents ou par rapport a une requete. SMART (System for the Mechanical Analysis and Retrieval of Text) [4] est l’un des premiers systemes de recherche a avoir adopte cette approche. Plusieurs ameliorations des systemes de recherche d’information utilisent les relations semantiques qui existent entre les termes dans un document. LSI (Latent Semantic Indexing) [5], par exemple realise ceci a travers des methodes d’analyse qui mesurent la cooccurrence entre deux termes dans un meme contexte, tandis que Hearst et Morris [6] utilisent des thesaurus en ligne pour creer des liens semantiques entre les termes dans un processus de chaines lexicales. Dans ces travaux nous developpons un nouveau systeme de recherche qui permet de representer les donnees textuelles par des signaux. Cette nouvelle forme de representation nous permettra par la suite d’appliquer de nombreux outils mathematiques de la theorie du signal, tel que les Transformees en ondelettes et jusqu’a aujourd’hui inconnue dans le domaine de la recherche d’information textuelle
A prototype of simple image database system for dermatological clinics

Electronic health record systems are gradually coming into use at large hospitals, but are not yet implemented at clinics. This is due to both expensive costs and a lack of interest in checking electronic health records on the part of patients. And doctors also do not want to show patients their health records, as it then may require a doctor to let patients observe images to check for improvement of symptoms at follow-up. In this paper, we propose a simple web based database system of affected part images that both doctors and patients can check. In the prototype system, doctors can photograph affected part areas and tagging keywords such as patient's ID, patient's name, affected part name and prescription. The system is implemented on smartphone and tablet for both doctors and patients quickly and easily use during medical examination in the clinic.
FADD Deficiency Impairs Early Hematopoiesis in the Bone Marrow

Signal transduction mediated by Fas-associated death domain protein (FADD) represents a paradigm of coregulation of apoptosis and cellular proliferation. During apoptotic signaling induced by death receptors including Fas, FADD is required for the recruitment and activation of caspase 8. In addition, a death receptor-independent function of FADD is essential for embryogenesis. In previous studies, FADD deficiency in embryonic stem cells resulted in a complete lack of B cells and dramatically reduced T cell numbers, as shown by Rag1−/− blastocyst complementation assays. However, T-specific FADD-deficient mice contained normal numbers of thymocytes and slightly reduced peripheral T cell numbers, whereas B cell-specific deletion of FADD led to increased peripheral B cell numbers. It remains undetermined what impact an FADD deficiency has on hematopoietic stem cells and progenitors. The current study analyzed the effect of simultaneous deletion of FADD in multiple cell types, including bone marrow cells, by using the IFN-inducible Mx1-cre transgene. The resulting FADD mutant mice did not develop lymphoproliferation diseases, unlike Fas-deficient mice. Instead, a time-dependent depletion of peripheral FADD-deficient lymphocytes was observed. In the bone marrow, a lack of FADD led to a dramatic decrease in the hematopoietic stem cells and progenitor-enriched population. Furthermore, FADD-deficient bone marrow cells were defective in their ability to generate lymphoid, myeloid, and erythroid cells. Thus, the results revealed a temporal requirement for FADD. Although dispensable during lymphopoiesis post lineage commitment, FADD plays a critical role in early hematopoietic stages in the bone marrow.
The works of Thomas Carlyle

Title Type the works of thomas carlyle PDF the works of thomas carlyle volume 23 wilhelm meisteraposs apprenticeship and PDF sartor resartus thomas carlyle PDF the french revolution a history thomas carlyle PDF thomas carlyle a study of his literary apprenticeship 1814 1831 PDF the works of thomas deloney PDF beyond the spanish tragedy a study of the works of thomas kyd PDF the works of thomas de quincey part i 7 vols PDF the works of thomas jefferson correspondence 1789 1792 vol 6 PDF the works of thomas hood comic and serious in prose and verse with all the original illustrations PDF thomas carlyleaposs works history of friedrich ii of prussia called frederick the great PDF at risk liz carlyle 1 stella rimington PDF never lie to a lady neville family 1 liz carlyle PDF carlyle the french revolution continuum histories 5 PDF the collected works of samuel taylor coleridge vol 16 poetical works part 1 poems 2 vol set PDF gospel plays operas and later dramatic works collected works of langston hughes vol 6 PDF the works of john dryden poetical works PDF st thomas christians in madhya pradesh a historical study on apostolic church of st thomas PDF a president in the family thomas jefferson sally hemings and thomas woodson PDF instructors solutions manual vector calculus thomas h barr thomas h bar PDF thomas and percy and the dragon thomas and friends step into reading PDF thomas e thomas edward finegan book PDF the complete psychological works of sigmund freud quot the ego and the id quot and other works PDF instructors solutions manual vector calculus thomas h barr thomas h barr PDF instructor39s solutions manual vector calculus thomas h barr thomas h barr PDF when art really works PDF how the mind works PDF manufacturing works PDF how it works the aeroplane PDF how lcd tv works diagram PDF toilet how it works PDF complete works vol 1 PDF how basketball works PDF how language works PDF life and works of mn roy PDF the major works PDF selected works PDF strypeaposs works PDF writing that works PDF poetical works PDF
Clinical characteristics and treatment outcome in 65 cases with refractory cytopenia of childhood defined according to the WHO 2008 classification

This study analysed 65 children who were prospectively registered between 1999 and 2008 and fulfilled the World Health Organization 2008 criteria of refractory cytopenia of childhood (RCC). First‐line therapy was determined by the treating physicians: 25 patients received immunosuppressive therapy (IST), 12 patients received haematopoietic stem cell transplantation (HSCT) and one patient received intensive chemotherapy. The remaining 27 patients were followed without treatment for more than 2 years (watch and wait; WW). In the WW group, 18 patients had stable disease without further intervention. Thirteen of 29 patients (45%) who ended up receiving IST showed response. The combination of ciclosporin and antithymocyte globulin was not shown to be superior to ciclosporin alone with regard to response rate or survival. Of 28 patients who ended up undergoing HSCT, 17 patients are alive in complete remission, whereas nine patients died mostly due to transplantation‐related mortality. The 5‐year overall survival for all patients was 82 ± 5%. Eight patients suffered from disease progression. Patients with monosomy 7 or multilineage‐dysplasia had a significantly higher incidence of disease progression. This analysis revealed heterogeneity in the clinical course of RCC, varying from those who remained stable for long periods to those who progressed to advanced disease.
SUSCEPTIBILITY OF WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) TO EXPERIMENTAL INFECTION WITH EPIZOOTIC HEMORRHAGIC DISEASE VIRUS SEROTYPE 7

During the fall of 2006, in Israel, epizootic hemorrhagic disease virus (EHDV) serotype 7 caused an intense and widespread epizootic in domestic cattle that resulted in significant economic losses for the dairy industry. The susceptibility of potential North American vector and ruminant hosts to infection with EHDV-7 is not known but is essential to understanding the potential for establishment of this exotic orbivirus in North America if it were introduced. Our primary objective was to determine whether white-tailed deer (WTD; Odocoileus virginianus) are susceptible to infection with EHDV-7. Six, 8-mo-old WTD were experimentally infected with EHDV-7, and all became infected and exhibited varying degrees of clinical disease. Clinical signs, clinicopathologic abnormalities, and postmortem findings were consistent with previous reports of orbiviral hemorrhagic disease (HD) in this species. Four of six animals died or were euthanized because of the severity of disease, one on postinoculation day (PID) 5 and the remaining WTD on PID 7. All deer had detectable viremia on PID 3, which peaked on PID 5 or 6 and persisted for as long as PID 46 in one animal. Deer surviving the acute phase of the disease seroconverted by PID 10. Based on the 67% mortality rate we observed, this strain of EHDV-7 is virulent in WTD, reaffirming their role as a sentinel species for the detection of endemic and nonendemic EHDV. Further, the observed disease was indistinguishable from previous reports of disease caused by North American EHDV and bluetongue virus serotypes, highlighting the importance of serotype-specific diagnostics during suspected HD outbreaks.
Pediatric in-intensive-care-unit cardiac arrest: Incidence, survival, and predictive factors*

Objective:To describe the incidence, survival, and neurologic outcome of in–intensive-care-unit (ICU) cardiac arrest and to identify factors predictive of survival to hospital discharge. Methods:We performed a retrospective cohort study. Eligible patients were <18 yrs of age and experienced a cardiac arrest during their admission to a multidisciplinary pediatric intensive care unit in the 5.5-yr period ending June 2002. Cardiac arrest was defined as the administration of chest compressions or defibrillation for a nonperfusing cardiac rhythm. Mortality and the Paediatric Cerebral Performance Score were measured and presented according to the Utstein style. Factors predictive of survival to hospital discharge were identified by univariate analysis and independent predictors were identified by multivariate analysis. Main Measurements and Results:Ninety-one children had cardiac arrest, yielding an incidence of 0.94 cardiac arrests per 100 admissions. Resuscitation was successful in 75 (82%) children, 61 (67%) survived 24 hrs, 25 (27%) children survived to ICU discharge and 23 (25%) to hospital discharge. At hospital discharge, the median Pediatric Cerebral Performance Category score was 2 (range, 1–3) and the median Pediatric Overall Performance Category score was 3 (range, 1–4). No child was assessed as normal on both scores. The independent positive predictors of hospital mortality were the presence of renal failure before cardiac arrest (odds ratio [OR], 6.1; 95% confidence interval [CI], 1.8–31), being on epinephrine infusion at time of cardiac arrest (OR, 9.5; 95% CI, 1.5–62), and the administration of one or more calcium boluses during resuscitation (OR, 5.4; 95% CI, 1.1–25). The use of extracorporeal membrane oxygenation (ECMO) within 24 hrs after cardiac arrest was associated with reduced hospital mortality (OR, 0.18; 95% CI, 0.04–0.76). Conclusions:In-ICU cardiac arrest is associated with high in-hospital mortality and subsequent morbidity in survivors. Prearrest renal dysfunction and epinephrine infusion were associated with increased in-hospital mortality. The use of post-arrest ECMO within 24 hrs was associated with reduced mortality. Rigorous prospective evaluation of the role of ECMO following cardiac arrest is needed.
Association of PTPN22 haplotypes with Graves' disease.

CONTEXT A recent study reported associations of a series of single nucleotide polymorphisms (SNPs) within PTPN22, including rs2476601, with rheumatoid arthritis. OBJECTIVE Having previously reported significant association of the T allele of rs2476601 in a Graves' disease (GD) cohort, we sought to determine whether novel rheumatoid arthritis-associated SNPs were also contributing to susceptibility to GD. DESIGN Case control and family-based studies of five PTPN22 tag SNPs were performed. SETTING An United Kingdom academic department of medicine was the setting for the study. PATIENTS OR OTHER PARTICIPANTS A total of 768 GD patients, 768 control subjects, and 313 families with autoimmune thyroid disease participated. MAIN OUTCOME MEASURE Tests for association with disease were the main outcome measure. RESULTS No association with disease of any of the individual SNPs and no correlation between genotype and clinical phenotype were seen. However, haplotype analysis of the SNP markers with addition of rs2476601 did reveal a strong association of a haplotype containing the T allele, in both the case control (chi2 = 29.13; P = 6.77 x 10(-8)) and family data sets (chi2 = 5.24; P = 0.02). Furthermore, a novel protective effect of a haplotype containing all six SNPs was observed (chi2 = 17.02; P = 3.7 x 10(-5)). CONCLUSIONS These data suggest that the association of SNPs within the PTPN22 region differs between autoimmune diseases, occurring individually and/or as part of a haplotype, indicating that the mechanisms by which PTPN22 confers susceptibility to GD may, in part, be disease specific.
miXGENE Tool for Learning from Heterogeneous Gene Expression Data Using Prior Knowledge

High-throughput genomic technologies have proved to be useful in the search for both genetic disease markers and more complex predictive and descriptive models. By the same token, it became obvious that accurate and interpretable models need to concern more than raw measurements taken at a single phase of gene expression. In order to reach a deeper understanding of the molecular nature of complexly orchestrated biological processes, all the available measurements and existing genomic knowledge need to be fused. In this paper, we introduce a tool for machine learning from heterogeneous gene expression data using prior knowledge. The tool is called miXGENE, it is elaborated upon in close connection with the biological departments that dispose of the above-mentioned data and have a strong interest in their integration within particular problem-oriented projects. The main idea is not merely to capture the transcriptional phase of gene expression quantified by the amount of messenger RNA (mRNA). The increasing availability of microRNA (miRNA) data asks for its concurrent analysis with the transcriptional data. Moreover, epigenetic data such as methylation measurements can help to explain unexpected transcriptional irregularities. miXGENE is an environment for building workflows that enable rapid prototyping of integrative molecular models.
No alterations in exon 21 of the RBI gene in sarcomas and carcinomas of the breast, colon, and lung

Studies of mutant genotypes of the retinoblastoma susceptibility gene (RBI) in different solid tumors have mainly been concentrated on the demonstration of loss of heterozygosity (LOH) at both internal and external polymorphic sites. One reason for this is the complex organization of the gene. The p105RB protein has been shown to interact with both DNA and regulatory cellular proteins and oncoproteins. The amino acids encoded by exon 21 are implicated in several of these interactions. Both point mutations and intragenic deletions involving exon 21 have previously been reported in human tumors. We have examined RBI exon 21 from a number of human tumor types where significant LOH in or around the RBI gene has been reported. DNA from 78 primary tumors was amplified using the polymerase chain reaction (PCR) with primers covering exon 21, followed by constant denaturant gel electrophoresis (CDGE). The 78 tumors included 11 breast carcinomas, 30 nonsmall cell lung carcinomas, 6 colon carcinomas, and 31 sarcomas. The small cell lung cancer cell line NCI‐H209, previously shown to harbour a point mutation in codon 706: TGT‐ > TTT (Cys‐ > Phe), was detected using CDGE. Apart from this control mutant cell line, we did not detect any mutations in the examined region in any of the tumors.
Lower Plasma Levels and Accelerated Clearance of High Density Lipoprotein (HDL) and Non-HDL Cholesterol in Scavenger Receptor Class B Type I Transgenic Mice*

Recent studies have indicated that the scavenger receptor class B type I (SR-BI) may play an important role in the uptake of high density lipoprotein (HDL) cholesteryl ester in liver and steroidogenic tissues. To investigate the in vivo effects of liver-specific SR-BI overexpression on lipid metabolism, we created several lines of SR-BI transgenic mice with an SR-BI genomic construct where the SR-BI promoter region had been replaced by the apolipoprotein (apo)A-I promoter. The effect of constitutively increased SR-BI expression on plasma HDL and non-HDL lipoproteins and apolipoproteins was characterized. There was an inverse correlation between SR-BI expression and apoA-I and HDL cholesterol levels in transgenic mice fed either mouse chow or a diet high in fat and cholesterol. An unexpected finding in the SR-BI transgenic mice was the dramatic impact of the SR-BI transgene on non-HDL cholesterol and apoB whose levels were also inversely correlated with SR-BI expression. Consistent with the decrease in plasma HDL and non-HDL cholesterol was an accelerated clearance of HDL, non-HDL, and their major associated apolipoproteins in the transgenics compared with control animals. These in vivo studies of the effect of SR-BI overexpression on plasma lipoproteins support the previously proposed hypothesis that SR-BI accelerates the metabolism of HDL and also highlight the capacity of this receptor to participate in the metabolism of non-HDL lipoproteins.