Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Nitric Oxide and Noradrenergic Activation in the Posterior Hypothalamus on Arterial Pressure Tolerance to Nitroglycerin in Rats

The effects of nitric oxide (NO) and noradrenergic activation in the posterior hypothalamus on arterial pressure tolerance induced by subcutaneous injection of nitroglycerin (NTG) was investigated in anesthetized Sprague-Dawley rats. Intravenous injections of NTG (3, 10, and 30 µg/kg) and sodium nitroprusside (1, 3, and 10 µg/kg) produced dose-dependant decreases in arterial blood pressure. Tolerance to NTG was produced by subcutaneous administration of 4.0 mg of NTG as 4 separate hourly injections of 1.0 mg each, affecting the dose-dependent response of NTG IV injection. The 4 high-dose NTG pulse injections produced a marked shift in the dose—response curves for arterial pressure depression induced by intravenous injection of the challenge doses of NTG, but did not alter hypotensive responses to sodium nitroprusside. The tolerance responses to arterial pressure depression were enhanced by a bilateral microinjection of NTG (1 nmol) and by diethylamine NONOate (1 nmol), an NO donor, into the posterior hypothalamus. Bilateral microinjection of guanethidine (1.5 nmol), a noradrenergic blocker, into the posterior hypothalamus inhibits NTG tolerance in a period of time within 2 hours. We conclude that exogenous NO and noradrenergic activation in the posterior hypothalamus play an important role in arterial pressure tolerance to systemically administered NTG.
Neoadjuvant cisplatin and bevacizumab in triple negative breast cancer (TNBC): Safety and efficacy.

551 Background: We have previously shown that neoadjuvant cisplatin has activity in TNBC, a subtype of breast cancer for which there is no effective targeted therapy. Bevacizumab adds to the efficacy of chemotherapy in metastatic breast cancer; however, there is limited data on the safety or efficacy of bevacizumab in combination with chemotherapy in the neoadjuvant or adjuvant setting. Thus, we sought to explore the safety and efficacy of the addition of bevacizumab to cisplatin in the treatment of TNBC. METHODS 51 patients (pts) with confirmed TNBC provided informed consent and were enrolled in a single arm phase II trial of neoadjuvant cisplatin 75 mg/m2 q 3 weeks x 4 cycles and bevacizumab 15 mg/kg q 3 weeks x 3 cycles prior to definitive surgery. Only 3 cycles of bevacizumab were delivered to allow 6 weeks between the last dose of bevacizumab and surgery. Research biopsies were obtained and breast MRI performed before treatment and at surgery. Postoperatively, pts received doxorubicin and cytoxan (AC) plus bevacizumab or AC/Taxol plus bevacizumab. Median age was 50 yrs (range 30 to 66 yrs); tumors were clinical T1 (2%), T2 (80%), T3 (18%). RESULTS Forty-six pts are evaluable for response and 5 pts are still receiving neoadjuvant therapy. Clinical responses to date: 12/46 (26%) clinical complete response (cCR), 24/46 (52%) clinical partial response (cPR), 5/46 (11%) stable disease (SD), and 1/46 (2%) progressive disease (PD). Non-responders included 4/46 (9%) pts who discontinued protocol therapy for toxicity. To date, 7/46 (15%) pts achieved a complete pathological response (Miller-Payne 5) and an additional 10/46 (22%) were Miller-Payne 4. Five pts did not complete neoadjuvant therapy, 2 with tinnitus/hearing loss and 3 with grade 4 toxicities consisting of refractory hypertension in 1 pt and pulmonary embolism (PE) in 2 pts. Tissue-based assays to predict platinum/bevacizumab responses, including BRCA1/2 status, are underway. CONCLUSIONS Cisplatin and bevacizumab has some activity in TNBC as demonstrated by 37% of evaluable pts with a Miller-Payne 4 or 5 pathological response. However, toxicity, including tinnitus/hearing loss, hypertension and PE, limited completion of neoadjuvant therapy in 11% of pts. No significant financial relationships to disclose.
Potent Effects of the Total Saponins from Dioscorea nipponica Makino Against Streptozotocin‐Induced Type 2 Diabetes Mellitus in Rats

The aim of the present paper was to investigate the effects and possible mechanisms of the total saponins from Dioscorea nipponica Makino (TSDN) against type 2 diabetes mellitus. Streptozotocin (STZ) with high‐fat diet induced type 2 diabetes mellitus (T2DM) rats were treated with TSDN. Some biochemical parameters, target proteins and genes were investigated. The results showed that TSDN decreased the levels of food/water intake, fasting blood glucose and serum lipid parameters, ameliorated oral glucose and insulin tolerance test levels, markedly increased body weight and serum insulin, reduced excess free radicals and affected ossification and renal protection. Histopathological examination indicated that TSDN increased liver glycogen, decreased the production of lipid vacuoles and lightened liver damage. Further investigation showed that TSDN down‐regulated the protein expressions of NF‐κB, GRP78, ATF6, eIF2 and the levels of MAPK phosphorylation and up‐regulated the protein expressions of IRS‐1, GLUT‐4, p‐Akt and p‐AMPK. In addition, TSDN obviously decreased the gene expressions of TNF‐a, IL‐6, PEPCK, G6Pase, GSK‐3β and GSK‐3β activity, and increased the gene expressions of PFK, PK and GK activity. These findings show the anti‐diabetic activity of total saponins from D. nipponica Makino, which should be developed as a new potent drug for treatment of diabetes mellitus in future. Copyright © 2014 John Wiley & Sons, Ltd.
Gender Differences in Health Literacy Among Korean Adults

The role of gender in determining the level of health literacy in Korean adults is unclear. This study aimed to investigate the level of health literacy in Korean adults and identify factors associated with health literacy by gender. This study employed a cross-sectional survey design with a convenient sample of 585 community-dwelling Korean adults age19 years and older. Health literacy was measured by using eight items selected from Chew et al.’s 16-question self-reported health literacy measure. In accordance with Andersen’s health behavior model, predisposing, enabling, and need factors were included in the multiple regression model. Women indicated a higher level of health literacy than men in understanding medical forms, directions on medication bottles, and written information offered by health care providers. Additionally, for Korean women, a higher level of health literacy was associated with attaining a higher education level and having a consistent place to receive care. Unmarried men and men who had higher self-rated health reported a higher level of health literacy compared with their counterparts. Lower level of depression and higher monthly income were significantly linked to a higher level of health literacy in both men and women. This study has established the importance of gender differences in health literacy and suggests gender-specific intervention may be warranted to reduce the existing gap in health literacy in both Korean men and women. Future research should replicate this study to confirm whether or not our finding is an international phenomenon.
Generation and characterization of diesel engine combustion emissions from petroleum diesel and soybean biodiesel fuels and application for inhalation exposure studies

Abstract Biodiesel made from the transesterification of plant- and animal-derived oils is an important alternative fuel source for diesel engines. Although numerous studies have reported health effects associated with petroleum diesel emissions, information on biodiesel emissions are more limited. To this end, a program at the U.S. EPA assessed health effects of biodiesel emissions in rodent inhalation models. Commercially obtained soybean biodiesel (B100) and a 20% blend with petroleum diesel (B20) were compared to pure petroleum diesel (B0). Rats and mice were exposed independently for 4 h/day, 5 days/week for up to 6 weeks. Exposures were controlled by dilution air to obtain low (50 µg/m3), medium (150 µg/m3) and high (500 µg/m3) diesel particulate mass (PM) concentrations, and compared to filtered air. This article provides details on facilities, fuels, operating conditions, emission factors and physico-chemical characteristics of the emissions used for inhalation exposures and in vitro studies. Initial engine exhaust PM concentrations for the B100 fuel (19.7 ± 0.7 mg/m3) were 30% lower than those of the B0 fuel (28.0 ± 1.5 mg/m3). When emissions were diluted with air to control equivalent PM mass concentrations, B0 exposures had higher CO and slightly lower NO concentrations than B100. Organic/elemental carbon ratios and oxygenated methyl esters and organic acids were higher for the B100 than B0. Both the B0 and B100 fuels produced unimodal-accumulation mode particle-size distributions, with B0 producing lower concentrations of slightly larger particles. Subsequent papers in this series will describe the effects of these atmospheres on cardiopulmonary responses and in vitro genotoxicity studies.
Measuring the effects of reverberation and noise on sentence intelligibility for hearing-impaired listeners.

PURPOSE The Speech Transmission Index (STI; Houtgast, Steeneken, & Plomp, 1980; Steeneken & Houtgast, 1980) is commonly used to quantify the adverse effects of reverberation and stationary noise on speech intelligibility for normal-hearing listeners. Duquesnoy and Plomp (1980) showed that the STI can be applied for presbycusic listeners, relating speech reception thresholds (SRTs) in various reverberant conditions to a fixed, subject-dependent STI value. The current study aims at extending their results to a wider range of hearing-impaired listeners. METHOD A reverberant analogue of the SRT is presented--the speech reception reverberation threshold (SRRT)--which determines the amount of reverberation that a listener can sustain to understand 50% of the presented sentences. SRTs are performed and evaluated in terms of STI for 5 normal-hearing participants and 36 randomly selected hearing-impaired participants. RESULTS Results show that differences in STI between reverberant and noisy conditions are only small, equivalent to a change in speech-to-noise ratio < 1.3 dB. CONCLUSION The STI appears to be a convenient, single number to quantify speech reception of hearing-impaired listeners in noise and/or reverberation, regardless of the nature of the hearing loss. In future research, the SRRT may be applied to further investigate the supposed importance of cognitive processing in reverberant listening conditions.
Development and Validation of Next Generation Blended Learning Environment Questionnaire for Senior High School Students

In the past years, the use of Learning Management Systems (LMSs) has been regarded as an advantageous means for handling administrative duties in classroom instructions rather than being successful in facilitating learning. Although several studies have reported that the utilization of certain LMSs have resulted to positive effects on students' achievement and perceptions, the LMS's capabilities and limitations make it limited for teachers to develop new pedagogical approaches in maximizing its use in promoting effective and efficient teaching-learning processes. This study aimed to develop and validate a scale for evaluating a digital environment when using LMSs in supporting learning directly. The developed Next Generation Blended Learning Environment Questionnaire (NxGBLEQ) is a four-point Likert scale and originally had 52 questions. For further validation, 4 Science Education experts validated the questionnaire, and it was administered to 326 senior high school students. Consistent with the adopted theoretical framework, the 35-item NxGBLEQ comprised 5 factors: (1) interoperability and integration; (2) personalization; (3) analytics, advising, and learning assessment; (4) collaboration, and (5) accessibility and universal design. The entire scale and factors demonstrated a satisfactory level of internal reliability, and senior high school students could use it.
Participation of the C-Terminal Domain of RNA Polymerase II in Exon Definition during Pre-mRNA Splicing

ABSTRACT Interaction between transcription and pre-mRNA processing via binding of polymerase II (Pol II) to factors involved in capping, splicing, and polyadenylation has recently been demonstrated. The C-terminal domain (CTD), a highly phosphorylated repeat sequence of the largest subunit of Pol II, has been implicated in this interaction because deletion of this domain affects downstream RNA processing events and because it is the binding site for numerous processing factors. Here we show that recombinant CTD, free of other components of Pol II, activated in vitro splicing and assembly of the spliceosome in nuclear extracts if, and only if, the assayed precursor RNA was recognized via exon definition, i.e., if the substrates contained complete exons with both 3′ and 5′ splice sites. Furthermore, depletion of intact Pol II inactivated splicing of this set of precursor RNAs and addition of recombinant CTD restored activity. The added recombinant CTD was quickly hyper- and hypophosphorylated in extract, became associated with the precursor RNA, and stimulated the association of U1 snRNPs but not ASF/SF2 with substrate RNA. These observations suggest that the mode of interaction between the CTD and splicing factors is integrally tied to exon definition and the mechanism whereby distal exons can be recognized and brought into juxtaposition during assembly of the spliceosome.
Ideology and Sexuality: Rape Laws in Qing China

It is generally accepted that Qing China (1644–1912) was a straitlaced, sexually repressed society. Robert H. van Gulik, for example, ended his study of sexual life in China with the fall of the Ming dynasty, in part because he believed that Chinese attitudes toward sexuality became much more repressive after the Ming, and the generalizations he made in his book were not appropriate for the Qing (1961:333–36). This dramatic change in attitude has been attributed to the resurgence of Cheng- Zhu Neo-Confucianism, with its strict view of sexual relations in general, and female sexuality in particular (Ropp 1981:120–24).
Validity and limitations of self-reported periodontal health.

OBJECTIVES To study the agreement between self-reported dental conditions and clinical findings in an adult population (20-84 years of age), and thus evaluate questionnaires as a tool in epidemiological studies of oral health, in general, and periodontal health, in particular. MATERIAL AND METHODS A questionnaire was sent to 900 randomly selected subjects in the age groups 20-29, 50-59 and 75-84 years. Of these, 723 subjects (81.0%) answered the questionnaire and 20% of them underwent a clinical examination. RESULTS As regards the remaining teeth, there was a mean difference of 1.4 teeth between the number indicated in the questionnaire and that found on the clinical examination. This difference was most marked in the older subjects. Eight of the nine subjects with removable dentures reported in their answers that they had removable dentures. Periodontal variables--we found significantly more subjects with pathological gingival pockets among those who stated that they had pockets than among those who answered that they did not (P = 0.01; chi-square independent test). Gingival bleeding was common in those who answered 'Yes' than in those who answered 'No' to the question concerning bleeding gums. This difference was significant (P = 0.05; chi-square independent test) in the three age groups. However, there was no correlation between the questionnaires and the clinical examination concerning tooth mobility. CONCLUSION Questionnaires concerning oral status are valid concerning the number of remaining teeth and use of removable dentures. They are less reliable about specific periodontal variables, but can still become a valuable tool for epidemiological studies of periodontal health.
Plant regeneration through somatic embryogenesis from zygotic embryo-derived callus of Areca catechu L. (Arecaceae)

SummaryAn in vitro culture procedure was established for somatic embryogenesis and plant regeneration from callus cultures of the important palm ‘betel nut’ (Areca catechu L.). Segments of zygotic embryos of Areca catechu L. were cultured on Murashige and Skoog basal medium supplemented with dicamba (9.05, 18.1, 27.15, and 36.2 μM). After 7–8 wk in darkness, wounded regions of explants formed callus with yellow, soft, glutinous structures. Proliferation and maintenance of callus was on the same dicamba-containing medium. With regular subculture every 8 wk, the callus showed pale yellow, compact and nodular structures. During subculture, somatic embryos were formed spontaneously from nodular callus tissues within 2–4 mo. The embryos developed into plantlets after 10 wk of culture on basal medium free of plant growth regulators. After subculturing every month for 3 mo. , the plantlets were transferred to containers for acclimatization in the greenhouse. The survival rate was 24%.
Exploring the Link Between Test Suite Quality and Automatic Specification Inference

While no one doubts the importance of correct and complete specifications, many industrial systems still do not have formal specifications written out -- and even when they do, it is hard to check their correctness and completeness. This work explores the possibility of using an invariant extraction tool such as Daikon to automatically infer specifications from available test suites with the idea of aiding software engineers to improve the specifications by having another version to compare to. Given that our initial experiments did not produce satisfactory results, in this paper we explore which test suite attributes influence the quality of the inferred specification. Following further study, we found that instruction, branch and method coverage are correlated to high recall values, reaching up to 97.93%.
Evaluation of the Role of Multidrug Resistance-Associated Protein (Mrp) 3 and Mrp4 in Hepatic Basolateral Excretion of Sulfate and Glucuronide Metabolites of Acetaminophen, 4-Methylumbelliferone, and Harmol in Abcc3–/– and Abcc4–/– Mice

Although glucuronide and sulfate conjugates of many drugs and endogenous compounds undergo appreciable hepatic basolateral excretion into sinusoidal blood, the mechanisms that govern basolateral translocation of these hydrophilic metabolites have not been completely elucidated. In the present study, the involvement in this process of Mrp3 and Mrp4, two basolateral efflux transporters, was evaluated by analyzing the hepatic basolateral excretion of the glucuronide and sulfate metabolites of acetaminophen, 4-methylumbelliferone, and harmol in Abcc3–/– and Abcc4–/– mice using a cassette dosing approach. In the livers of Abcc3–/– and Abcc4–/– mice, the basolateral excretory clearance of acetaminophen sulfate was reduced ∼20 and ∼20%, 4-methylumbelliferyl sulfate was reduced ∼50 and ∼65%, and harmol sulfate was decreased ∼30 and ∼45%, respectively. The basolateral excretory clearance of acetaminophen glucuronide, 4-methylumbelliferyl glucuronide, and harmol glucuronide was reduced by ∼96, ∼85, and ∼40%, respectively, in the livers of Abcc3–/– mice. In contrast, basolateral excretory clearance of these glucuronide conjugates was unaffected by the absence of Mrp4. These results provide the first direct evidence that Mrp3 and Mrp4 participate in the hepatic basolateral excretion of sulfate conjugates, although additional mechanism(s) are likely involved. In addition, they reveal that Mrp3 mediates the hepatic basolateral excretion of diverse glucuronide conjugates.
Role of the NFKB1 −94ins/delATTG promoter polymorphism in IBD and potential interactions with polymorphisms in the CARD15/NOD2, IKBL, and IL‐1RN genes

Background: Recently, an association of the NFKB1 polymorphism −94ins/delATTG with ulcerative colitis (UC) has been reported. This 4‐bp insertion/deletion polymorphism is localized in the promoter region of the NFKB1 gene and appears to be functionally relevant. The aim of the present study was to confirm the association of the −94ins/delATTG (W/D) NFKB1 promoter polymorphism with UC in a population of German origin and to test for a potential association with Crohn's disease (CD). Furthermore, potential interactions of the −94ins/delATTG polymorphism with the IKBL and the IL‐1RN genes should be determined. Materials and Methods: The study population comprised 630 patients with CD, 365 patients with UC, and 974 healthy controls. Genotyping was performed using polymerase chain reaction and restriction fragment length polymorphism analysis. For statistical evaluation, the chi‐square test and the Fisher exact test were used. Results: No significant association of the W/D NFKB1 polymorphism with CD or UC was detected. In addition, no significant interactions between the −94ins/delATTG NFKB1 polymorphism and polymorphisms within the IKBL and the IL‐1RN genes, respectively, were found in CD or UC. Also, no significant interactions of the NFKB1 polymorphism with mutations of the CARD15/NOD2 gene and with clinical phenotypes were detected in CD. Moreover, no associations of the NFKB1 polymorphism were found in UC depending on disease localization. Conclusions: The present study could not confirm the reported association of the −94ins/delATTG NFKB1 polymorphism with UC and also found no evidence for a role of this polymorphism in CD. The results do not give evidence for a role of this NFKB1 polymorphism in the pathogenesis of UC and CD.
Traffic Camera Anomaly Detection

Detection of camera anomaly and tampering have attracted increasing interest in video surveillance for real-time alert of camera malfunction. However, the anomaly detection for traffic cameras monitoring vehicles and recognizing license plates has not been formally studied and it cannot be solved by existing methods. In this paper, we propose a camera anomaly detection method for traffic scene that has distinct characteristics of dynamics due to traffic flow and traffic crowd, compared with normal surveillance scene. Image quality used as low-level features are measured by no-referenced metrics. Image dynamics used as mid-level features are computed by histogram distribution of optical flow. A two-stage classifier for the detection of anomaly is devised by the modeling of image quality and video dynamics with probabilistic state transition. The proposed approach is robust to many challenging issues in urban surveillance scenarios and has very low false alarm rate. Experiments are conducted on real-world videos recorded in traffic scene including the situations of high traffic flow and severe crowding. Our test results demonstrate that the proposed method is superior to previous methods on both precision rate and false alarm rate for the anomaly detection of traffic cameras.