Miracle-dz/UniFAR-FTUs huggingface dataset:
Developmentally Dictated Expression of Heat Shock Factors: Exclusive Expression of HSF4 in the Postnatal Lens and Its Specific Interaction with αB-crystallin Heat Shock Promoter*

The molecular cascade of stress response in higher eukaryotes commences in the cytoplasm with the trimerization of the heat shock factor 1 (HSF1), followed by its transport to the nucleus, where it binds to the heat shock element leading to the activation of transcription from the down-stream gene(s). This well-established paradigm has been mostly studied in cultured cells. The developmental and tissue-specific control of the heat shock transcription factors (HSFs) and their interactions with heat shock promoters remain unexplored. We report here that in the rat lens, among the three mammalian HSFs, expression of HSF1 and HSF2 is largely fetal, whereas the expression of HSF4 is predominantly postnatal. Similar pattern of expression of HSF1 and HSF4 is seen in fetal and adult human lenses. This stage-specific inverse relationship between the expression of HSF1/2 and HSF4 suggests tissue-specific management of stress depending on the presence or absence of specific HSF(s). In addition to real-time PCR and immunoblotting, gel mobility shift assays, coupled with specific antibodies and HSE probes, derived from three different heat shock promoters, establish that there is no HSF1 or HSF2 binding activity in the postnatal lens nuclear extracts. Using this unique, developmentally modulated in vivo system, we demonstrate 1) specific patterns of HSF4 binding to heat shock elements derived from αB-crystallin, Hsp70, and Hsp82 promoters and 2) that it is HSF4 and not HSF1 or HSF2 that interacts with the canonical heat shock element of the αB-crystallin gene.
Effects of Flow Cytometric Analysis and Cell Sorting on Photosynthetic Carbon Uptake by Phytoplankton in Cultures and from Natural Populations

Photosynthetic rates of phytoplankton were significantly lower after analysis by flow cytometry (FCM) than before. Exposure to the laser beam during the sorting process caused significant physiological damage. The cellular content of a radiolabel accumulated prior to FCM was not affected by FCM. Although it may not be possible to use FCM to preconcentrate cells for further physiological studies, samples may be incubated with stable or radioactive isotopes and then analyzed by FCM.
Altered EBV Viral Load Setpoint after HIV Seroconversion Is in Accordance with Lack of Predictive Value of EBV Load for the Occurrence of AIDS-Related Non-Hodgkin Lymphoma1

In contrast to the situation in the post-transplant setting, in HIV-infected individuals an elevated EBV load is not predictive of EBV-related malignancies. To study whether a high EBV load is already a normal situation early in HIV infection and is not related to a decrease in immune function over time, we investigated EBV load and EBV-specific CD8+ T cells ∼1 year before and 1 year after HIV seroconversion. EBV load significantly increased after HIV seroconversion from 205 to 1002 copies/106 PBMC (p < 0.001), whereas no further increase in EBV load was observed between 1 and 5 years after HIV seroconversion (median, 1827–2478 copies/106 PBMC; p = 0.530). Interestingly, the absolute number of EBV lytic epitope, RAKFKQLL-specific CD8+ T cells increased over HIV seroconversion (4.78 to 9.54/μl; p = 0.011). Furthermore, the fraction of CD27-negative effector, RAK-specific CD8+ T cells tended to increase (from 12.2 to 17.31% CD27−; p = 0.051), in accordance with Ag-driven differentiation. In conclusion, both virological and immunological data support the idea that a new EBV viral setpoint is reached early in HIV infection, probably by EBV reactivation, as suggested by the preferential increase in EBV lytic epitope-specific CD8+ T cells. These data may thus help to explain the lack of predictive value of EBV load for the occurrence of AIDS-related lymphoma.
Characteristics and Correlates of Caregivers’ Perceptions of Their Family Members’ Memory Loss

Background Understanding caregiver’s perceptions of their family member’s memory loss is a necessary step in planning nursing interventions to detect and address caregiver burden. Objective The purpose of this study was to characterize caregivers’ perceptions of their family members’ memory loss and identify potential correlates within Leventhal’s common sense model (CSM). Methods This secondary analysis used baseline data from a larger randomized controlled trial. Patients with memory loss and their caregivers (N = 83 dyads) from the community were included. The adapted Brief Illness Perception Questionnaire (BIPQ) assessed caregivers’ illness perceptions. Eight additional instruments measured correlates within the CSM. Responses were described; multiple linear regression was used to predict BIPQ dimension scores, and logistic regression was used to predict dichotomized BIPQ scores. Results Most caregivers were female, White, and spouses of the patients; they reported a range of perceptions on the nine BIPQ dimensions. Patients’ cognitive function consistently emerged as a significant correlate of caregivers’ illness perceptions, explaining the most variance in caregivers’ perceived consequences, identity, and treatment control (p < .01). Caregivers’ reactions to patients’ behavioral symptoms and caregivers’ trait anxiety were associated with perceived illness coherence (p < .01). Caregivers with higher severity of daily hassles and White caregivers perceived that their family members’ memory loss would last longer (p < .001). Discussion Caregivers’ perceptions of family members’ memory loss varied; distinct dimensions of caregivers’ illness perception were associated with a range of clinical and psychosocial factors. This exploratory study demonstrates the complexity of applying the CSM to caregivers of persons with memory loss.
Neurophysiology of Rett Syndrome

Rett syndrome is a neurodevelopmental disorder that in most cases is consequent to a mutation in the MECP2 gene. The central nervous system is the primary organ system involved in Rett syndrome. Neurophysiologic evaluations provide information concerning the developmental aspects of Rett syndrome and the character and extent of involvement of the central, peripheral, and autonomic nervous system pathways. Evoked potentials typically demonstrate intactness of peripheral auditory and visual pathways and suggest dysfunction of central or “higher” cortical pathways. Somatosensory evoked potentials can be characterized by “giant” responses, suggesting cortical hyperexcitability. Cortical hyperexcitability is further suggested by the findings of the electroencephalogram (EEG), which are primarily characterized by a loss of expected developmental features; the appearance of focal, multifocal, and generalized epileptiform abnormalities; and the occurrence of rhythmic slow (theta) activity, primarily in the frontal-central regions. Epileptic seizures are reported to occur frequently in Rett syndrome. However, many events presumed to be seizures have no EEG correlate during video-EEG monitoring. Impairment of the autonomic nervous system in Rett syndrome is suggested by an increased incidence of long Q—T intervals during electrocardiographic recordings and diminished heart rate variability. Autonomic nervous system dysfunction can contribute to the increased incidence of sudden unexpected death in Rett syndrome. (J Child Neurol 2005;20:740—746).
Strong Associations Exist among Oxidative Stress and Antioxidant Biomarkers in the Circulating, Cellular and Urinary Anatomical Compartments in Guatemalan Children from the Western Highlands

Background A series of antioxidant enzymes and non-enzymatic compounds act to protect cells from uncontrolled propagation of free radicals. It is poorly understood, though, to what extent and how their interaction is harmonized. Objectives To explore associative interactions among a battery of urinary and blood biomarkers of oxidative stress and enzymatic and non-enzymatic markers of the antioxidant defense system in children from low income households. Methods For this cross-sectional descriptive study, urine, red cells, and plasma were sampled in 82 preschool children attending three daycare centers in Quetzaltenango Guatemala. The urinary oxidative stress biomarkers studied were F2-isoprostanes and 8-hydroxy-deoxy-guanosine. Red cell enzyme activities measured were: catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase. Circulating non-enzymatic antioxidants selected were: retinol, tocopherols, β-carotene and coenzymes Q9 and Q10. Results In a Spearman rank-order correlation hemi-matrix, of 55 paired combinations of the 11 biomarkers, 28 (51%) were significantly correlated among each other (p≤ 0.05), with the strongest association being retinol and tocopherols (r = 0.697, p<0.001), and 4 associations (9%) showed a trend (p> 0.5 to ≤ 0.10). F2-isoprostanes showed the greatest number of cross-associations, having significant interactions with 8 of the 10 remaining biomarkers. Goodness-of-fit modeling improved or maintained the r value for 24 of the significant interactions and for one of the 5 borderline associations. Multiple regression backward stepwise analysis indicated that plasma retinol, β-carotene and coenzyme Q10 were independent predictors of urinary F2-isoprostanes. Conclusion Numerous significant associations resulted among biomarkers of oxidation and responders to oxidation. Interesting findings were the apparent patterns of harmonious interactions among the elements of the oxidation-antioxidation systems in this population.
An investigation of the effect of route to university on UK Business School students’ experiences of a professional mentoring scheme

This thesis presents a Bourdieusian analysis of a mentoring scheme in order to understand whether it may develop social capital at the individual level. Differential rates in the acquisition of sought after graduate jobs suggest that higher education is not facilitating fair access to what is a limited supply of graduate-entry, high status, well-paid careers. As such, there is a need to understand how universities might best support their students who come from groups traditionally underrepresented in higher education in obtaining graduate level employment. This thesis seeks to make a contribution to knowledge in this area; it offers an evaluation of the effectiveness of a mentoring scheme run in a Russell Group University Business School in preparing students for and connecting them with the world of work. The research aims to create knowledge about how students from different backgrounds experience and benefit from mentoring by business professionals. It tests the application of Bourdieu’s theoretical framework to the professional mentoring scheme and makes some proposals as to how Bourdieu’s theories might be refined. The insights gained from the study are used to offer suggestions for the design of future mentoring schemes to ensure that they optimise value to students from non-traditional backgrounds.
Full-color computational holographic near-eye display

ABSTRACT Near-eye displays (NEDs) are an excellent candidate for the future of augmented reality. Conventional micro-display based NED designs mostly provide stereoscopic 3D experience, which leads to visual discomfort due to vergence-accommodation conflict. Computational holographic near-eye displays (HNEDs) can simultaneously provide wide FOV, retinal resolution, attractive form-factor, and natural depth cues including accommodation. In HNEDs, computer-generated holograms (CGHs) are displayed on a spatial light modulators (SLMs). We propose a CGH computation algorithm that applies to arbitrary paraxial optical architectures; where the SLM illumination beam can be collimated, converging or diverging, and the SLM image as seen by the eye box plane may form at an arbitrary location, and can be virtual or real. Our CGH computation procedure eliminates speckle noise, which is observed in all other laser-based displays, and chromatic aberrations resulting from the light sources and the optics. Our proof-of-concept experiments demonstrate that HNEDs with simple optical architectures can deliver natural 3D images within a wide FOV (70 degrees) at retinal resolution (30 cycles-per-degree), exceeding 4000 resolvable pixels on a line using a printed binary mask. With the advances in SLM technology, HNEDs can realize the ultimate personalized display, meeting the demand of emerging augmented and virtual reality applications.
Investment Bank Reputation and IPO Qualifications

In this paper, we examines the influence of investment bank’s reputation and political connection on the IPO qualification of Chinese firm, The results show that firms which sponsored by prestigious investment banks more likely pass the regular’s screening process, the investment bank’s political connection have no significant impact on the IPO qualification. We further compared the investment bank’s customer characteristic, and find that the customers of prestigious investment bank are more large-scale, high growth companies, and more central enterprises, indicate that high reputation investment bank tend choose low risk and high growth prospective IPO companies in order to avoid damage to reputation.
Sinusoidal Servocompensator Implementations With Real-Time Requirements and Applications

This brief solves critical long-standing numerical stability issues for solutions to the sinusoidal robust servomechanism problem implemented as discrete-time linear time-invariant transfer functions; new closed-form results for direct form I and direct form II implementations now enable such forms to be used indefinitely in open-loop autonomous, or feedforward, configuration, and also provide difference equation initialization and servocompensator output parameter estimation methods that can be quickly calculated and used to enhance transient system performance and to assess harmonic control components. Simulation and real-time experimental results presented, respectively, exhibit expected concordance and robust effectiveness, and therefore highlight the method whereby such linear configurations can be pragmatically applied to further reduce computational implementation requirements relative to their mathematically intensive nonlinear time-varying counterparts.
Anticholinergic medications for managing noisy respirations in adult hospice patients.

PURPOSE Anticholinergic medications for reducing noisy respirations in adult hospice patients are evaluated. SUMMARY Anticholinergic medications used to reduce noisy respirations from retained secretions in terminal patients include atropine, glycopyrrolate, scopolamine, and scopolamine derivatives. Pharmaceutical anticholinergic treatment of retained secretions in hospice patients was evaluated in six studies, three of which compared the efficacy of glycopyrrolate to scopolamine in actively dying patients. Subcutaneous glycopyrrolate, scopolamine hydrobromide, and scopolamine butylbromide were similar in their ability to reduce noisy respirations overall and lower and the level of distress exhibited by family members and visitors. Two of the six studies compared the efficacy of medication therapy after institutional formulary changes from scopolamine to glycopyrrolate. The same dosages of subcutaneous glycopyrrolate and scopolamine, which delivered an initial bolus followed by continuous infusion, were reported in each study. Both studies concluded that there was equivalent efficacy between the two products. One study reported a more rapid response in patients treated with glycopyrrolate. In comparison, the last study reported more rapid responses in patients who received scopolamine compared with patients who received glycopyrrolate. Retrospective reports described symptom improvement with parenteral scopolamine in most patients. CONCLUSION Parenteral and transdermal anticholinergic medications are useful for the reduction of noisy respirations in hospitalized hospice patients. Difficult administration makes oral and sublingual products less desirable for use in this population.
A dynamic music adding system based on cloud computing

With the popularization of mobile devices and cloud storages, people tend to store photos and videos through internet. Recently many cloud systems start providing functions to produce reviewing films from stored media files. However, the background music of the film usually does not match the video's emotion because the added music is usually selected randomly. This work proposed an emotion-based music adding cloud service that generates a reviewing film with the appropriate background music fitting the emotion of the corresponding video.
Encounters with an Open Mind: A Relational Grounding for Neighborhood Governance

Abstract Neighborhood governance has become a widespread approach to improving the quality of life in cities. The idea is that sustained interactions between public professionals and residents will better meet the needs of local areas and people. However, neighborhood working approaches purporting to provide tailor-made policies and solutions tend to perpetuate habitual practices and hegemonic institutions of hierarchy and competition. This chapter enquires how conditions can be created for different kinds of conversations and relationships to emerge that lead to innovative practices and sustainable change. I argue that public professionals need not only interact extensively with residents but should also engage in encounters with an open mind. Empirically illustrated with an innovative approach to neighborhood working in Amsterdam (the Netherlands), I explain how they can go beyond habitual practices by letting new shared views and actions emerge in-between them. Doing so fosters deeper institutional transformations toward a relational grounding for urban governance and public administration.
Inorganic polyphosphate and exopolyphosphatase in the nuclei of Saccharomyces cerevisiae: dependence on the growth phase and inactivation of the PPX1 and PPN1 genes

Nuclei of the yeast Saccharomyces cerevisiae possess inorganic polyphosphates (polyP) with chain lengths of ca. 10–200 phosphate residues. Subfractionation of the nuclei reveals that the most part of polyP is not associated with DNA. Transition of the yeast cells from stationary phase to active growth at orthophosphate (Pi) excess in the medium is followed by the synthesis of the shortest polyP (<15 phosphate residues) and hydrolysis of the high‐molecular polyP (>45 phosphate residues) in the nuclei. Nuclear exopolyphosphatase (exopolyPase) activity does not depend on the growth phase. The PPX1 gene encoding the major cytosolic exopolyPase does not encode the nuclear one and its inactivation has no effect on polyP metabolism in this compartment. Under inactivation of the PPN1 gene encoding another yeast exopolyPase, elimination of the nuclear exopolyPase is observed. The effect of PPN1 inactivation on the polyP level in the nuclei is insignificant in the stationary phase, while in the exponential phase this level increases 2.3‐fold as compared with the parent strain of S. cerevisiae. In the active growth phase, no hydrolysis of high‐molecular polyP is detected while the synthesis of short‐chain polyP is retained. The data obtained indicate substantial changes in polyP metabolism in nuclei under the renewal of active growth, which only partially depends on the genes of polyP metabolism known to date. Copyright © 2006 John Wiley & Sons, Ltd.
Bilateral Total Hip Arthroplasty: One Stage Versus Two Stage Procedure

The purpose of this study was to determine if any differences existed in the early complication rate, short term clinical outcome, and total length of hospital stay between patients who had bilateral total hip arthroplasty performed under a single anesthetic (during 1 patient visit to the operating room) and patients who had the procedure performed under 2 anesthetics (during 2 patient visits to the operating room). Patients operated on bilaterally were divided into 3 groups: Group A (1 stage procedure)-hips that were operated on simultaneously (128 hips); Group B (2 stage procedure)-surgeries performed less than 6 weeks apart (126 hips); and Group C (2 stage procedure)-surgeries performed between 6 weeks and 6 months apart (256 hips). All patients were evaluated after an average followup of 1.5 years. There were no differences in operative, early local, or general complications among the 3 groups. In particular, no higher incidence of pulmonary embolism or deep vein thrombosis was found in the 1 stage group. Preoperatively, very stiff hips (total range of motion < 50 °) gained significantly more motion in the 1 stage group than in the 2 stage groups, whereas hips with better preoperative motion (total range of motion > 50 °) improved the most in Group B, without a significant difference occurring between Groups A and C. The degree of pain reduction was the same in all groups, but patients in the 1 stage group had a significantly better capacity for walking after their procedure. Average total hospital stay was 5 to 6 days less for the patients in Group A than those in the other groups, which, combined with using the operating room only once, resulted in a reduction of overall hospital costs by more than 30% when using the 1 stage procedure.
Topical Reviews on Target of Rapamycin Signaling Networks The Role of Target of Rapamycin Signaling Networks in Plant Growth and Metabolism 1

The target of rapamycin (TOR) kinase, a master regulator that is evolutionarily conserved among yeasts (Saccharomyces cerevisiae), plants, animals, and humans, integrates nutrient and energy signaling to promote cell proliferation and growth. Recent breakthroughs made possible by integrating chemical, genetic, and genomic analyses have greatly increased our understanding of the molecular functions and dynamic regulation of the TOR kinase in photosynthetic plants. TOR signaling plays fundamental roles in embryogenesis, meristem activation, root and leaf growth, flowering, senescence, and life span determination. The molecular mechanisms underlying TOR-mediated ribosomal biogenesis, translation promotion, readjustment of metabolism, and autophagy inhibition are now being uncovered. Moreover, monitoring photosynthesis-derived Glc and bioenergetics relays has revealed that TOR orchestrates unprecedented transcriptional networks that wire central metabolism and biosynthesis for energy and biomass production. In addition, these networks integrate localized stem/progenitor cell proliferation through interorgan nutrient coordination to control developmental transitions and growth.
Mpi recombinase globally modulates the surface architecture of a human commensal bacterium

The mammalian gut represents a complex and diverse ecosystem, consisting of unique interactions between the host and microbial residents. Bacterial surfaces serve as an interface that promotes and responds to this dynamic exchange, a process essential to the biology of both symbionts. The human intestinal microorganism, Bacteroides fragilis, is able to extensively modulate its surface. Analysis of the B. fragilis genomic sequence, together with genetic conservation analyses, cross-species cloning experiments, and mutational studies, revealed that this organism utilizes an endogenous DNA inversion factor to globally modulate the expression of its surface structures. This DNA invertase is necessary for the inversion of at least 13 regions located throughout the genome, including the promoter regions for seven of the capsular polysaccharide biosynthesis loci, an accessory polysaccharide biosynthesis locus, and five other regions containing consensus promoter sequences. Bacterial DNA invertases of the serine site-specific recombinase family are typically encoded by imported elements such as phage and plasmids, and act locally on a single region of the imported element. In contrast, the conservation and unique global regulatory nature of the process in B. fragilis suggest an evolutionarily ancient mechanism for surface adaptation to the changing intestinal milieu during commensalism.