Miracle-dz/UniFAR-FTUs huggingface dataset:
N-Glycan Modification in Aspergillus Species

ABSTRACT The production by filamentous fungi of therapeutic glycoproteins intended for use in mammals is held back by the inherent difference in protein N-glycosylation and by the inability of the fungal cell to modify proteins with mammalian glycosylation structures. Here, we report protein N-glycan engineering in two Aspergillus species. We functionally expressed in the fungal hosts heterologous chimeric fusion proteins containing different localization peptides and catalytic domains. This strategy allowed the isolation of a strain with a functional α-1,2-mannosidase producing increased amounts of N-glycans of the Man5GlcNAc2 type. This strain was further engineered by the introduction of a functional GlcNAc transferase I construct yielding GlcNAcMan5GlcNac2 N-glycans. Additionally, we deleted algC genes coding for an enzyme involved in an early step of the fungal glycosylation pathway yielding Man3GlcNAc2 N-glycans. This modification of fungal glycosylation is a step toward the ability to produce humanized complex N-glycans on therapeutic proteins in filamentous fungi.
Novel Association Measures Using Web Search with Double Checking

A web search with double checking model is proposed to explore the web as a live corpus. Five association measures including variants of Dice, Overlap Ratio, Jaccard, and Cosine, as well as Co-Occurrence Double Check (CODC), are presented. In the experiments on Rubenstein-Goodenough's benchmark data set, the CODC measure achieves correlation coefficient 0.8492, which competes with the performance (0.8914) of the model using WordNet. The experiments on link detection of named entities using the strategies of direct association, association matrix and scalar association matrix verify that the double-check frequencies are reliable. Further study on named entity clustering shows that the five measures are quite useful. In particular, CODC measure is very stable on word-word and name-name experiments. The application of CODC measure to expand community chains for personal name disambiguation achieves 9.65% and 14.22% increase compared to the system without community expansion. All the experiments illustrate that the novel model of web search with double checking is feasible for mining associations from the web.
Application of the J Concept to Fatigue Crack Growth in Large-Scale Yielding

Fatigue crack propagation tests were performed on compact tension specimens of AISI 316 steel, in the elastic-plastic and plastic regions. The parameter AJ was chosen to characterize propagation. Results show that there is a Paris law type correlation between propagation rate and AJ. An effect on propagation rate, which is attributed to the R = P m i n /P m a x ratio, has been noted. A numerical verification of the applicability of the AJ concept was determined by use of a theoretical equation. These results were compared with experimental results.
Microstructural characterization of thick YBa_2Cu_3O_7−δ films on improved rolling-assisted biaxially textured substrates

The microstructural changes associated with the reduced dependence of critical current density (Jc) versus thickness of thick, epitaxial YBa_2Cu_3O_7–δ (YBCO) films on rolling-assisted biaxially textured substrates (RABiTS) were investigated. Pulsed laser deposited YBCO films varying in thickness from 1.0 to 6.4 ? m on RABiTS with an architecture of Ni–3 at. % W/Y_2O_3/yttrium-stabilized-zirconia/CeO_2/YBCO were prepared for cross-sectional transmission electron microscopy studies. Dramatic improvements in physical properties and microstructural quality were observed resulting from the use of Ni–3 at. % W substrates, which provided a sharper texture over earlier Ni substrates, and replacement of CeO_2 with Y_2O_3 as the seed layer within the buffers. The YBCO films showed exceptional orientation up to 6.4 μm thickness, with no misoriented grains or dead layers observed and only limited reaction between the YBCO and CeO_2 cap layer. The high quality of the films was also attributed in part to the formation of a tungsten oxide layer forming at the top of the Ni–3% W substrate, limiting the growth of deleterious NiO into the conductor.
Performance Evaluation of Semi Precast Concrete Beam-Column Connections with U-Shaped Strands

In this study, a new precast concrete (PC) beam-column joint of moment resisting frame applicable for moderate seismic regions is proposed. A semi PC beam-column connection with U-shaped strands is developed in an attempt to improve workability and provide effective stress transfer mechanism at the joint. The structural system consists of PC beams with U-shaped strands, PC columns, PC slabs, and topping concrete. A series of three interior and three exterior semi PC joint specimens was tested to investigate the structural behavior of the system subjected to the lateral cyclic load. Key test variables are the number of strands placed in the PC beam and the presence or absence of the transverse reinforcements at the connection. The experiment and performance evaluation of the system were conducted in accordance with ACI T1.1–01 (2001). According to the test results, the proposed structural system with transverse reinforcements at the joint is sufficient to use in moderate seismic regions.
Task-resource co-allocation for hotspot minimization in heterogeneous many-core NoCs

To fully exploit the massive parallelism of many cores, this work tackles the problem of mapping large-scale applications onto heterogeneous on-chip networks (NoCs) to minimize the peak workload for energy hotspot avoidance. A task-resource co-optimization framework is proposed which configures the on-chip communication infrastructure and maps the applications simultaneously and coherently, aiming to minimize the peak load under the constraints of computation power and communication capacity and a total cost budget of on-chip resources. The problem is first formulated into a linear programming model to search for optimal solution. A heuristic algorithm is further developed for fast design space exploration in extremely large-scale many-core NoCs. Extensive simulations are carried out under real-world benchmarks and randomly generated task graphs to demonstrate the effectiveness and efficiency of the proposed schemes.
Early Application of Auxiliary Partial Orthotopic Liver Transplantation in Murine Model of Wilson Disease

Background Liver transplantation (LT) is the only option of treatment for Wilson disease (WD) when chelation therapy fails, but it is limited due to the shortage of donor. Auxiliary partial orthotopic LT (APOLT) has been performed successfully in end-stage WD patients, which expands the donor pool. Methods Atp7b−/−mice were used as experimental model of WD. Eight- and 20-week-old mice were used as different timepoints to perform APOLT. Serum copper, tissue copper, serum ceruloplasmin (CP), and liver histological examination were observed after operation. Results Hepatic and serum copper levels in Atp7b−/− mice decreased after APOLT, and copper metabolism disorder of WD mice was relieved at both early and late stages. The progression of pathology in the native liver was delayed only when transplantation was performed at an early stage. Conclusions Auxiliary partial orthotopic LT can significantly improve copper metabolism disorder in the Atp7b−/− mice, and early transplantation may prevent the disease progression.
Computing Neighbourhoods with Language Models in a Collaborative Filtering Scenario

Language models represent a successful framework for many Information Retrieval tasks: ad hoc retrieval, pseudo-relevance feedback or expert finding are some examples. We present how language models can compute effectively user or item neighbourhoods in a collaborative filtering scenario (this idea was originally proposed in [14]). The experiments support the applicability of this approach for neighbourhoodbased recommendation surpassing the rest of the baselines. Additionally, the computational cost of this approach is small since language models have been efficiently applied to large-scale retrieval tasks such as web search.
Toward a quantitative assessment of diffusion anisotropy

Indices of diffusion anisotropy calculated from diffusion coefficients acquired in two or three perpendicular directions are rotationally variant. In living monkey brain, these indices severely underestimate the degree of diffusion anisotropy. New indices calculated from the entire diffusion tensor are rotationally invariant (RI). They show that anisotropy is highly variable in different white matter regions depending on the degree of coherence of fiber tract directions. In structures with a regular, parallel fiber arrangement, water diffusivity in the direction parallel to the fibers (D| ≈ 1400–1800 × 10−6 mm2/s) is almost 10 times higher than the average diffusivity in directions perpendicular to them ((D + D⊥′)/2 ≈ 150–300 × 10−6 mm2/s), and is almost three times higher than previously reported. In structures where the fiber pattern is less coherent (e.g., where fiber bundles merge), diffusion anisotropy is significantly reduced. However, RI anisotropy indices are still susceptible to noise contamination. Monte Carlo simulations show that these indices are statistically biased, particularly those requiring sorting of the eigenvalues of the diffusion tensor based on their magnitude. A new intervoxel anisotropy index is proposed that locally averages inner products between diffusion tensors in neighboring voxels. This “lattice” RI index has an acceptably low error variance and is less susceptible to bias than any other RI anisotropy index proposed to date.
Composite materials contribution in strengthening concrete structures affected by alkali–aggregate reaction

Alkali–aggregate reactivity (AAR) is a chemical reaction that occurs in some concrete structures. AAR is a reaction between pore solution alkali hydroxide and some siliceous aggregates. The damage to the concrete induced by AAR is very important with expansion and cracking. The main purpose of this investigation is to evaluate the effectiveness of the use of external bonded FRP on the durability of civil engineering structures damaged by alkali reaction (mechanical behaviour determination, comparison with Larive's model and confinement efficiency). In a first approach, we are working on a mortar specimen containing alkali–silica aggregates. The deterioration of mortar is determined from the expansion measures and from the measure of mechanical properties. To reduce the necessary duration of development of alkali–aggregate reaction, two accelerated mortar bar tests are evaluated: immersion in saturated NaCl solution at 50°C; immersion at 38°C (P 18 585 AFNOR standard). Two types of sample are made: no reac.
Biochemical Abnormalities of the Third Component of Complement in Neonates

ABSTRACT: The third component of complement, C3, is of central importance as an opsonin in the nonimmune host. Although gestational deficiencies in C3 levels are well recognized in neonates, defects in complement-mediated functions have not in every case correlated with low levels of complement proteins. Because opsonic functions of C3 are mediated through a reactive thiolester bond, we hypothesized that a biochemical dysfunction at this active site could explain the newborn's predisposition to infection, even with relatively normal C3 levels. We therefore examined the biochemical integrity of the C3 thiolester in an assay independent of all other complement proteins. As measured by ELISA, mean C3 levels from 44 neonates (24-43 wk) were significantly lower in infants < 30 wk gestational age (0.79 ± 0.13 mg/mL) than in full-term newborns (1.19 ± 0.27 mg/ml, p < 0.05). Furthermore, biochemical reactivity of the thiolester bond, as measured by incorporation of the radiolabeled nucleophile, methylamine, correlated significantly with gestational age (r = 0.45, p < 0.01). Functional C3 was defined as the product of thiolester reactivity and C3 level; 9/11 premature and 2/17 full-term infants had levels of functional C3 which were less than 50% of the adult norm. Structural analysis of neonatal C3 revealed the two-chain structure in all neonates; four neonates had an additional band at 205 kD which may represent an impairment in posttranslational processing of a precursor molecule. We conclude that defects in thiolester reactivity may constitute a newly identified mechanism for the newborn's susceptibility to infection.
Fault TolerantTopologyControl for All-to-One and One-to-All Communicationin WirelessNetworks

This paper intr oduces the problem of fault tolerant topology control for all-to-one and one-to-all communication in static wir eless networks with asymmetric wir elesslinks. This problem is important in both theoretical and practical aspects.We investigate two approaches, namely minimum weight based approach and nearest neighbor augmentation approach, to address this problem. Specifically, for eachapproach,we proposethr ee algorithms for thr ee interpretations of the problem: 1) construct -node disjoint paths fr om every node to a specificroot node,2) construct -node disjoint paths fr om the root node to every other node, 3) construct -node disjoint paths fr om the root node to every other node and also fr om every node to the root node. Furthermor e, we give theoretical analysis for all six algorithms. Among other results, we show that the minimum weight based approach has a -approximation algorithm for all-to-one fault tolerant topology control where is the number of disjoint paths. When , this approach solves the minimum power all-to-one connected topology control problem. Thr ough extensi ve simulations, we demonstrate that minimum weight based approach performs better for the first two interpretations and the augmentation basedapproachperforms better for the third interpretation. To the bestof our knowledge,this paper is the first to study the fault tolerant topologycontrol for all-to-one and one-to-all communication in asymmetric static wir elessnetworks, and alsois the first to demonstrate that the minimum power all-to-one -connectedtopology control problem has an optimal solution.
dq0-modeling of interior permanent-magnet synchronous machines for high-fidelity model order reduction

This paper presents a dq0 flux-linkage based electrical model for permanent-magnet synchronous machines. To obtain the relationship between dq0 flux-linkages and currents, a series of magnetostatic finite-element calculations for various dq0 currents at different rotor positions is carried out. This dataset has then to be inverted to avoid having to derive the flux-linkages in Simulink, thus improving the numerical stability of the model. The model was validated using a 12/10 delta- and star-connected fractional-slot concentrated-winding permanent magnet synchronous machine presenting 3rd-order harmonics in the back-emf. Results show that considering only a fraction of the rated current for the 0-sequence component allows to take circulating currents in a delta-connected machine into account, improving the accuracy of the model compared to a model based on the d-and q-axis alone. Torque and qd0 current waveforms resulting from the presented model are comparable to the ones obtained with finite-element both in terms of average value and harmonic content.
Some Operators that Preserve the Locality of a Pseudovariety of Semigroups

It is shown that if 𝖵 is a local monoidal pseudovariety of semigroups, then 𝖪 ⓜ 𝖵, 𝖣 ⓜ 𝖵 and are local. Other operators of the form 𝖹 ⓜ(_) are considered. In the process, results about the interplay between operators 𝖹 ⓜ(_) and (_) * 𝖣k are obtained.
WebTLab: A cooccurrence-based approach to KBP 2010 Entity-Linking task

In this paper we describe the approach followed and results obtained in the KBPEntity Linking task of TAC 2010 by the Web Technologies Laboratory (WebTLab). The system implemented for the task is based on the intuition that instances in a knowledge base typically cooccur in documents with other related instances (for example, Obama and USA). Based on that intuition, the proposed system takes advantage of the information provided by the Wikipedia link structure to compute instance cooccurrence estimates, and uses them for disambiguation purposes, combinined with more classical techniques from the information retrieval community.
Dynamic Model Building of Metering Process for Pneumatic Plate-Type Vegetable Seed Metering Device

For designing high performance pneumatic plate-type vegetable metering device,dynamic models of seeds during metering process were established.Combined with experiments study,main factors of seeding effects were discussed.The absorption effect can be enhenced by increasing inlet air flow volume of the suction hole,reducing rotation speed of the metering device,increasing sliding friction coefficient between seeds and the seed metering device,and decreasing arm length of the seed metering device.For insuring the seeds can be absorbed stably to their metering position,vacuum degree and diameter of the suction hole should be increased properly for avoiding difficulty of ejection.Increasing static friction characteristics between seeds and seed metering device,reducing rotation speed of the metering device,decreasing arm length of the seed metering device,and properly controlling seed quantity are helpful for stable suction of seeds.When ejecting seeds,the ejection height should be as small as possible,and intensity of the jet flow field should not be too large for preventing rebounding of seeds.
Gipr Is Essential for Adrenocortical Steroidogenesis; However, Corticosterone Deficiency Does Not Mediate the Favorable Metabolic Phenotype of Gipr−/− Mice

Glucose-dependent insulinotropic polypeptide (GIP) promotes glucose-dependent insulin secretion. However, GIP also enhances glucocorticoid secretion and promotes adiposity. Because obesity and diabetes are glucocorticoid dependent, we examined whether the effects of GIP on energy balance and glycemia are regulated by glucocorticoids using pharmacological activation of GIP receptor (GIPR) signaling with [d-Ala2]GIP in mice and in Y1 adrenocortical cells. Genetic elimination of GIPR activity was also studied in normal- and high-fat (HF)-fed Gipr-deficient (Gipr−/−) mice. [d-Ala2]GIP increased murine corticosterone levels in a GIPR-dependent manner. Conversely, basal corticosterone levels were reduced, whereas food deprivation resulted in significantly enhanced plasma corticosterone levels in Gipr−/− mice. [d-Ala2]GIP increased cAMP levels, activated extracellular signal\x{2013}related kinase (ERK)1/2, increased expression of steroidogenic genes, and increased neutral lipid storage in Y1GIPR cells. Gipr−/− adrenal glands demonstrated a twofold upregulation of the ACTH receptor mRNA and increased sensitivity to ACTH ex vivo. Although HF-fed Gipr−/− mice exhibited significantly lower plasma corticosterone, glucocorticoid-treated HF-fed Gipr−/− mice had similar energy balance and glycemia compared with Gipr+/+ controls. Hence, although the Gipr is essential for adrenal steroidogenesis and links HF feeding to increased levels of corticosterone, reduced glucocorticoid levels do not significantly contribute to the enhanced metabolic phenotypes in HF-fed Gipr−/− mice.
Tonsillectomy, high dose immunoglobulins, and cyclophosphamide in progressive IgA-nephropathy

In progressive IgA-nephropathy (IgAN) no established therapy exists. We have analyzed the clinical course of 40 patients with progressive IgAN with tonsillectomy combined with cyclophosphamide (TE/CTX), high dose immunoglobulins (IVIG), or cyclophosphamide pulses (CyP), and 8 untreated patients as historical controls. Serum creatinine >250 μmol/l at beginning, proteinuria >2.5 g/l, and age >51 years were significant predictors for end stage renal failure in Cox regression analysis. In linear regression analysis, the decline of renal function significantly decreased only in patients after CyP from 29.7% per year to 2.8% per year. Proteinuria significantly decreased only in patients after CyP from 1.3 g/l to 1.1 g/l. IVIG had no maintained long term effect on renal function, and TE/CTX had no significant influence on renal function. In Kaplan Meier analysis, patients with CyP had a significantly higher cumulative probability of renal survival compared to the other patients. In conclusion, TE/CTX is not recommended in patients with advanced, progressive IgAN, if not surgically indicated. IVIG had no long-term effect on progression of IgAN. CyP therapy is able to stop the loss of renal function and decreases proteinuria in patients with progressive IgAN.
The excitation controller with gain scheduling mechanism for synchronous generator control

The power systems, including the synchronous generators and power systems networks, are complex nonlinear systems with configuration and parameters which change through time. That leads to electromechanical oscillations occurring in that system. Thus synchronous generator excitation controller must be capable of providing appropriate stabilization signal over broad range of operating conditions and disturbances. In this paper, an excitation controller (EC) with gain scheduling mechanism is proposed to deal with those phenomena. This controller is designed and implemented based on the gain scheduling approach according to the power system stabilizer (PSS) and the conventional PID controller in the case of automatic voltage regulator (AVR). The proposed PSS accommodate the nonlinear nature of power systems and adopt with parametric gain surfaces its parameters to the actual operating conditions. It is parameterized with two stages tuned procedure based on the nonlinear optimization problem solving according to the linearized power system model. The obtained result shows that the proposed excitation controller with gain scheduling mechanism can provide effective electromechanical oscillation damping and lower control signal effort in comparison with the a classic excitation system through wide range of operating conditions.
Nucleolar behavior in regenerating liver of normal and whole-body-irradiated rats.

After partial removal of the liver in Wistar rats, the number of nucleoli in hepatocytes was found to be substantially reduced, especially in the periods preceding the outburst of mitoses. By delaying the appearance of mitotic waves by whole-body X-irradiation (1000 R) delivered prior to surgery, a more pronounced lowering of the nucleoli number was observed. Following each mitotic peak, developed within the first 50 hours after operation, the normal distribution of different nucleolar number categories was regained. With respect to the mechanism of this numerical reduction, some evidence is presented, indicating that either the whole or parts of nucleoli may be extruded in the extranuclear areas of regenerating liver cells. Planar measurements demonstrated an important nucleolar volume enlargement, which took place particularly in the whole-body-irradiated animals. The specific cytochemical tests employed have shown that the intranucleolar structures containing RNA and/or lysine-rich proteins increased in both number and size, predominantly after X-irradiation followed by partial hepatectomy, exceeding by far the amounts observed in unirradiated liver cell nucleoli. The internal structure of nucleoli, as revealed by toluidine blue-ammonium molybdate staining procedures, Methods A or B, was modified after whole-body irradiation, owing to the development of anisonucleolinosis. Some possible mechanisms which could be involved in the numerical reduction and/or nucleolar enlargement phenomena, as well as those incriminated in the appearance of anisonucleolinosis, are suggested and discussed.