Miracle-dz/UniFAR-FTUs huggingface dataset:
Prokaryotic Homologs of the Eukaryotic 3-Hydroxyanthranilate 3,4-Dioxygenase and 2-Amino-3-Carboxymuconate-6-Semialdehyde Decarboxylase in the 2-Nitrobenzoate Degradation Pathway of Pseudomonas fluorescens Strain KU-7

ABSTRACT The 2-nitrobenzoic acid degradation pathway of Pseudomonas fluorescens strain KU-7 proceeds via a novel 3-hydroxyanthranilate intermediate. In this study, we cloned and sequenced a 19-kb DNA locus of strain KU-7 that encompasses the 3-hydroxyanthranilate meta-cleavage pathway genes. The gene cluster, designated nbaEXHJIGFCDR, is organized tightly and in the same direction. The nbaC and nbaD gene products were found to be novel homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, respectively. The NbaC enzyme carries out the oxidation of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate-6-semialdehyde, while the NbaD enzyme catalyzes the decarboxylation of the latter compound to 2-aminomuconate-6-semialdehyde. The NbaC and NbaD proteins were overexpressed in Escherichia coli and characterized. The substrate specificity of the 23.8-kDa NbaC protein was found to be restricted to 3-hydroxyanthranilate. In E. coli, this enzyme oxidizes 3-hydroxyanthranilate with a specific activity of 8 U/mg of protein. Site-directed mutagenesis experiments revealed the essential role of two conserved histidine residues (His52 and His96) in the NbaC sequence. The NbaC activity is also dependent on the presence of Fe2+ but is inhibited by other metal ions, such as Zn2+, Cu2+, and Cd2+. The NbaD protein was overproduced as a 38.7-kDa protein, and its specific activity towards 2-amino-3-carboxymuconate-6-semialdehyde was 195 U/mg of protein. Further processing of 2-aminomuconate-6-semialdehyde to pyruvic acid and acetyl coenzyme A was predicted to proceed via the activities of NbaE, NbaF, NbaG, NbaH, NbaI, and NbaJ. The predicted amino acid sequences of these proteins are highly homologous to those of the corresponding proteins involved in the metabolism of 2-aminophenol (e.g., AmnCDEFGH in Pseudomonas sp. strain AP-3). The NbaR-encoding gene is predicted to have a regulatory function of the LysR family type. The function of the product of the small open reading frame, NbaX, like the homologous sequences in the nitrobenzene or 2-aminophenol metabolic pathway, remains elusive.
Underwater Photogrammetry for Archaeology

Archaeological excavations are often irreversibly destructive, so it is important to accompany them with detailed documentation reflecting the accumulated knowledge of the excavation site. This documentation is usually iconographic and textual. Graphical representations of archaeological sites such as drawings, sketches, watercolors, photographs, topography, and photogrammetry are indispensable for such documentation and are an intrinsic part of an archaeological survey. However, as pointed out by Olivier Buchsenschutz in the introduction to the symposium Images and archaeological surveys, in Arles, France, in 2007 (Buchsenschutz, 2007, Introduction page 5), even a very precise drawing only retains certain observations that support a demonstration, just as a speech retains only some arguments, but this choice is not usually explicit. This somewhat lays the foundation of this work: a survey is both a metrics document and an interpretation of the site by archaeologist.
Application of fuzzy five states control system base on phase plane in steer by wire system of loader

Based on research of the phase plane division control method, according to the structure of wheel loader steer by wire system and its work condition, the fuzzy five states control system specially designed for wheel loader steer by wire system is introduced in this paper. To verify its performance, the controller was simulated in MATLAB/SIMULINK based on the model of wheel loader steer by wire system. Simulation results indicate that, compared with five states controller, the fuzzy five states controller is more effective especially to the wheel loader steer by wire system with the simple control rules and good performance of rapid response, short transition time, less steady state error, good tracking performances and disturbance rejection abilities.
Design of tunable SPR on MEMS for displays

A tunable surface plasmonic resonance (SPR) driven by microelectromechanical system (MEMS) is designed in this work. Pitch tuning of SPR structures on elastomer filters various transmission of electromagnetic (EM) spectra. A color filter based on SPR in the visible range is proposed and evaluated at various strains induced by the MEMS operation before quantified by figure of merit. Distinguishable EM spectra realized with the proposed design implied a solution to single pixel (subpixel) full color display operated in a time-sequential manner.
Monthly Electric Energy Demand Forecasting Based on Trend Extraction

Medium-term electric energy demand forecasting is an essential tool for power system planning and operation, mainly in those countries whose power systems operate in a deregulated environment. This paper proposes a novel approach to monthly electric energy demand time series forecasting, in which it is split into two new series: the trend and the fluctuation around it. Then two neural networks are trained to forecast them separately. These predictions are added up to obtain an overall forecasting. Several methods have been tested to find out which of them provides the best performance in the trend extraction. The proposed technique has been applied to the Spanish peninsular monthly electric consumption. The results obtained are better than those reached when only one neural network was used to forecast the original consumption series and also than those obtained with the ARIMA method
Metadata of the chapter that will be visualized in OnlineFirst

Electrospinning is a simple and versatile method to produce fibers using charged polymer solutions. As drug delivery systems, electrospun fibers are an excellent choice because of easy drug entrapment, high surface area, morphology control and biomimetic characteristics. Various drugs and biomolecules can be easily encapsulated inside or on fiber surface either during electrospinning or through post-processing of the fibers. Multicomponent fibers have attracted special attention because new properties and morphologies can be easily obtained through the combination of different polymers. The factors that affect the drug release such as construct geometry and thickness, diameter and porosity, composition, crystallinity, swelling capacity, drug loading, drug state, drug molecular weight, drug solubility in the release medium, drug–polymer– electrospinning solvent interactions are discussed. Mathematical models of drug release from electrospun fibers are reviewed and strategies to attain zero-order release and control of burst stage are considered. Finally, some results concerning release control in bicomponent fibers composed of poly(E-caprolactone) and Lutrol F127 (poly(oxyethylene-b-oxypropylene-b-oxyethylene) are presented. The properties of the bicomponent fibers were studied in order to determine the effect of electrospinning processing on crystallinity, hydrophilicity and degradation. Acetazolamide and timolol maleate were loaded in the fibers in different concentrations in order to determine the effect of drug solubility in polymer, drug state, drug loading and fiber composition on morphology, drug distribution and release kinetics. Such electrospun drug eluting fibers can be used as basic elements of various implants and scaffolds for tissue regeneration.
Impact of compliance with proton pump inhibitors on NSAID treatment.

OBJECTIVE To assess the impact of patient compliance with proton pump inhibitor (PPI) therapy on nonsteroidal anti-inflammatory drug (NSAID) treatment duration and upper-gastrointestinal (GI) complications in patients with gastroesophageal reflux disease (GERD). STUDY DESIGN Retrospective cohort study. METHODS Study subjects were GERD patients receiving cotherapy with a PPI and a cyclooxygenase-2-selective (COX-2-selective) or nonselective NSAID. Patients compliant and noncompliant with PPI therapy were compared on NSAID treatment duration and incidence of upper-GI events. Kaplan-Meier analysis and a multivariate Cox proportional hazards model were used to compare durations of NSAID treatment, controlling for baseline characteristics. The incidences of GI events were compared using incidence rate and Poisson regression models. The analyses were conducted separately for patients taking COX-2-selective NSAIDs and those taking nonselective NSAIDs. RESULTS In both patient groups taking a COX-2-selective agent (n = 12,562; 70.9% compliant) and nonselective NSAID (n = 17,487; 69.9% compliant), mean NSAID treatment duration was significantly longer (84.0 days and 20.8 days longer, respectively) in PPI-compliant patients than in noncompliant patients. Compliance with PPI therapy was associated with a greater reduction in the incidence of GI events than noncompliance in both patients taking a COX-2-selective NSAID (6-fold vs 5-fold; P = .026) and patients taking a nonselective NSAID (8-fold vs 6-fold; P = .002). CONCLUSIONS In GERD patients receiving NSAIDs, those who were compliant with PPI therapy had a longer NSAID treatment duration and better GI tolerability than those who were noncompliant.
The Sprouty‐related protein, Spred‐1, localizes in a lipid raft/caveola and inhibits ERK activation in collaboration with caveolin‐1

Caveolin‐1 (Cav‐1) has been suggested to function as a negative regulator of mitogen‐stimulated proliferation and the Ras‐p42/44 ERK (MAP kinase) pathway in a variety of cell types. However, the molecular basis of this suppression has not been clarified. Spred/Sprouty family proteins are also negative regulators of the ERK pathway by interacting with Raf‐1. The Spred/Sprouty family proteins contain a cysteine‐rich (CR) domain at the C‐terminus, which is thought to be palmitoylated like Cav‐1 and necessary for membrane anchoring. In this study, we demonstrated that Spred‐1 localized in cholesterol‐rich membrane raft/caveola fractions and interacted with Cav‐1. To clarify the biological effect of Cav‐1/Spred‐1 interaction, we used hematopoietic cells that lacked expression of caveolins but expressed Spred‐1. Forced expression of Cav‐1 suppressed SCF‐ and IL‐3‐induced proliferation and ERK activation. Furthermore, forced expression of exogenous Spred‐1 in Cav‐1‐expressing cells further suppressed proliferation and ERK activation. These data suggest that Spred‐1 inhibits ERK activation in collaboration with Cav‐1.
Haloperidol treatment with chronically medicated residents: dose effects on clinical behavior and reinforcement contingencies.

Twenty residents, most of whom had previously received neuroleptic maintenance therapy, were assessed in a double blind, placebo-controlled, cross-over study of haloperidol. Haloperidol was administered in standardized doses of . 025 and . 05 mg/kg/day, for 3 weeks each. Clinical changes were confined to a slight reduction in ratings of stereotypic behavior and an increase in gross-motor activity under the high dose. Under reinforcement conditions, there was a marked tendency for instruction following performance to improve under the high dose condition. Subjects were divided according to degree of stereotypic behavior. Those with initially high levels of stereotypy tended to show a significantly more favorable response to haloperidol than did those with low levels of stereotypy.
High-Performance Pixelwise Readout Integrated Circuits for Microbolometer

In this paper, a pixelwise readout structure for a microbolometer is proposed. A current mirror gate modulation (CM) is employed as an input circuit to archive high responsivity and a current mode background suppression is used to reduce the integration capacitance. As a long integration time of more than 1 ms can be obtained using this pixelwise readout structure, the noise equivalent temperature difference (NETD) is 26 mK, which is much better than that of the conventional circuits, 67 mK.
Surgery for carcinoma of the breast in women over 70 years of age

A review of 100 women over 70 years of age treated for breast carcinoma is reported. The short‐ and long‐term results of treatment were evaluated with a median follow up of 4. 7 years. Due to our aggressive approach, only 11% of this group died from breast cancer, and the 5‐year survival rate was 51%, including all stages of the disease. The result of this study indicates that elderly women with breast cancer should be treated by surgical methods that offer the best chance of cure, regardless of patient's age.
Cumulative Mutations of Ubiquitin Acceptor Sites in Human Immunodeficiency Virus Type 1 Gag Cause a Late Budding Defect

ABSTRACT The p6 domain of human immunodeficiency virus type 1 (HIV-1) Gag has long been known to be monoubiquitinated. We have previously shown that the MA, CA, and NC domains are also monoubiquitinated at low levels (E. Gottwein and H. G. Krausslich, J. Virol. 79:9134-9144, 2005). While several lines of evidence support a role for ubiquitin in virus release, the relevance of Gag ubiquitination is unclear. To directly address the function of Gag ubiquitination, we constructed Gag variants in which lysine residues in the NC, SP2, and p6 domains were mutated to arginine either in individual domains or in combination. Using these mutants, we showed that in addition to MA, CA, NC, and p6, SP2 is also mono- or diubiquitinated at levels comparable to those of the other domains. Replacement of all lysine residues in only one of the domains had minor effects on virus release, while cumulative mutations in NC and SP2 or in NC and p6 resulted in an accumulation of late budding structures, as observed by electron microscopy analysis. Strikingly, replacement of all lysine residues downstream of CA led to a significant reduction in virus release kinetics and a fivefold accumulation of late viral budding structures compared to wild-type levels. These results indicate that ubiquitination of lysine residues in Gag in the vicinity of the viral late domain is important for HIV-1 budding, while no specific lysine residue may be needed and individual domains can functionally substitute. This is consistent with Gag ubiquitination being functionally involved in a transient protein interaction network at the virus budding site.
Cognitive Load Theory and the Format of Instruction

Cognitive load theory suggests that effective instructional material facilitates learning by directing cognitive resources toward activities that are relevant to learning rather than toward preliminaries to learning. One example of ineffective instruction occurs if learners unnecessarily are required to mentally integrate disparate sources of mutually referring information such as separate text and diagrams. Such split-source information may generate a heavy cognitive load, because material must be mentally integrated before learning can commence. This article reports findings from six experiments testing the consequences of split-source and integrated information using electrical engineering and biology instructional materials. Experiment 1 was designed to compare conventional instructions with integrated instructions over a period of several months in an industrial training setting. The materials chosen were unintelligible without mental integration. Results favored integrated instructions throughout th.
Solubility Enhancement of Indomethacin with Poly(amidoamine) Dendrimers and Targeting to Inflammatory Regions of Arthritic Rats

This work includes investigation on solubility enhancement of indomethacin (IND) in the presence of poly(amidoamine) (PAMAM) dendrimers and passive targeting of the PAMAM/IND complex so formed to the inflamed regions in an animal model. The complex formation was confirmed by infrared and 1H nuclear magnetic resonance spectroscopy methods. Solubility of IND in aqueous G4-PAMAM followed Higuchi's AN curve depending on pH of the solubilizing medium. The solubility was decreased upon addition of dendrimer to the IND saturated solution at various pH, indicating aggregation behavior of the PAMAM/IND complex and conforming to the Higuchi's AN solubility profile. The in vitro release of IND from the PAMAM/IND complex through a cellophane membrane, from a Franz diffusion cell, showed 79±3.2% drug release in 24 h. The drug release was further retarded in the presence of human serum albumin (HSA) suggesting the significance of complex HSA binding in altering in vivo behavior of the complex. Intravenous administration of the PAMAM/IND complex formulation in rats showed a two-compartment pharmacokinetic profile. Enhanced effective IND concentrations in the inflamed regions were obtained for the prolonged time period with the PAMAM/IND complex compared to the free drug in arthritic rats indicating preferred accumulation of IND to the inflamed region. The targeting efficiency of PAMAM/IND complex was 2.29 times higher compared to free drug. In contrast to the previous investigations, two interesting findings reported here are: (a) solubility behavior of IND in G4-PAMAM dendrimer deviates from linearity with increasing concentrations of dendrimer at acidic to neutral pH values and (b) inspite of lymphatic drainage, retention of PAMAM/IND complexes occurs at the inflammatory site.
Domain-Specific Design of 3 D Interaction Techniques : Designing Useful Virtual Environment Applications

Few production virtual environment (VE) applications involve complex three-dimensional (3D) interaction. Our long-term collaboration with architects and engineers in designing 3D user interfaces (3D UIs) has revealed some of the causes: existing interaction tasks and/or techniques are either too generic when isolated from the application context, or too specific to be reusable. We propose a new design approach called domain-specific design (DSD) that sits between the generic and specific design approaches, with an emphasis on using domain knowledge in 3D interaction techniques. We also describe an interaction design framework encompassing generic, domain-specific, and applicationspecific interaction tasks and techniques. This framework can be used by designers to think of ways to produce domain-specific interaction techniques. We present a particular DSD method, and demonstrate its use for the design of cloning techniques in a structural engineering application. Results
Manometric Calibration and Comparison of TonoLab and TonoPen Tonometers in Rats With Experimental Glaucoma and in Normal Mice

PurposeTo compare the TonoPen and TonoLab tonometers to each other and to manometrically set intraocular pressure (IOP) in the eyes of normal mice, normal rats, and rats with chronic IOP elevation. MethodsThe measurement of IOP by the TonoPen and TonoLab tonometers was made in 21 normal rat eyes, 10 normal mouse eyes, and 16 rats that had either 2 or 4-week experimental glaucoma. IOP was varied from 10 to 50 mm Hg in steps of 10 mm Hg under conditions in which the eye was either open or closed to the reservoir controlling IOP. ResultsIn normal rat eyes, TonoPen overestimated manometric IOP at 10 mm Hg and underestimated it by up to 6 mm Hg at higher IOP, whereas the TonoLab matched set IOP within 1 mm Hg. In glaucoma rat eyes, the TonoLab accurately reflected manometric IOP under open stopcock conditions (linear regression: y=0.99x −0.62, R2=0.98), whereas in the closed stopcock condition, IOP measured lower at the higher IOP levels (P=0.0059, paired t test). In uncannulated rat glaucoma eyes, the tonometer used first gave higher IOP [paired t test, P=0.015 (TonoLab first); P=0.005 (TonoPen first)]. In normal mouse eyes under the open stopcock condition, the TonoLab nearly matched manometric IOP (linear regression: y=0.98x+1.57, R2=0.98). ConclusionsIn mouse and rat eyes, including rats with chronic IOP elevation, the TonoLab accurately reflected manometrically set IOP in an efficient manner.
Using Critical Care Simulations to Prepare Nursing Students for Capstone Clinical Experiences

The purpose of this innovative learning strategy was to decrease students' fear and anxiety within the critical care environment before the initiation of a critical care capstone clinical experience by enhancing their familiarity of the critical care environment through deliberate practice and experiential learning. This will in turn increase student knowledge and competence in the critical care setting. Critical care units are often used during a capstone clinical experience to enhance critical thinking and clinical reasoning. Student preparation for these rotations, however, is varied and often inadequate. The resulting fear and anxiety impair learning and also contribute to an unsafe environment for this at-risk population. Before the capstone experience, students participated in 3 simulation experiences, each addressing a core concept of critical care nursing. Faculty engaged students during the simulations, while modeling the confidence and critical thinking of a critical care nurse. After both the critical care simulation and the capstone rotation, students stated that the simulation allowed them the time in a safe environment to critically think through the steps to care for critical patients before the capstone rotation. The experience provided them with increased confidence necessary to discharge the responsibilities of a critical care nurse—attention to critical thinking and reasoning. Mindfully constructed simulations with clear objectives help to inoculate the student against fears associated with high-risk patients. This decrease in fear and anxiety before a hands-on clinical experience may improve patient safety. More confident students are also better able to engage in both experiential and deliberate learning, resulting in a more enhanced and meaningful clinical experience.
Digital Music Instruments and sound expression

The main goal of this paper is to describe certain aspects of the construction of a prototype, while introducing generally some of the key issues resulting of the design of its interface. The word LeinKlang (from the German) means sound (Klang) screen (Leinwand). This project consists in the construction of a digital music instrument. Physically, it appears as a screen, against which users interact, by shadow or video projection, and behind it, intentionally out of view, a set of 24 small loudspeakers.
Assessing the Relationship between Budget Participation and Employees’ Performance of Public Universities in Ghana: a Case of University of Education

The study examined the relationship between budget participation and employees’ performance of public universities in Ghana using University of Education as the study area. It therefore seeks to explore the behavioral elements of budgeting process in the study area and determine budget participation relationship with employees’ performance and commitment to budget goal. The study employed the quantitative research methodology and used convenience and simple random sampling techniques to select 110 employees of the university as respondents. Data was analyzed using SPSS 16. The findings of the study showed that the relationship between budget participation and employees’ performance (attainment of budget goal) was positive though not significant connoting that budget participation alone cannot significantly influence attainment of budget goal. Despite that it cannot alone help an organization to achieve its budget goal; it was evident from the study that it serves as the foundation for the other behavioral elements to be realized in the budgeting process. The research therefore argued out strongly for policy makers to hammer on budget participation since it is the foundation of which the other behavioral elements to achieving budget goal revolves.