Miracle-dz/UniFAR-FTUs huggingface dataset:
The polyadenylation complex of Trypanosoma brucei: Characterization of the functional poly(A) polymerase

ABSTRACT The generation of mature mRNA in the protozoan parasite Trypanosoma brucei requires coupled polyadenylation and trans splicing. In contrast to other eukaryotes, we still know very little on components, mechanisms, and dynamics of the 3′ end-processing machinery in trypanosomes. To characterize the catalytic core of the polyadenylation complex in T. brucei, we first identified the poly(A) polymerase [Tb927.7. 3780] as the major functional, nuclear-localized enzyme in trypanosomes. In contrast, another poly(A) polymerase, encoded by an intron-containing gene [Tb927.3. 3160], localizes mainly in the cytoplasm and appears not to be functional in general 3′ end processing of mRNAs. Based on tandem-affinity purification with tagged CPSF160 and mass spectrometry, we identified ten associated components of the trypanosome polyadenylation complex, including homologues to all four CPSF subunits, Fip1, CstF50/64, and Symplekin, as well as two hypothetical proteins. RNAi-mediated knockdown revealed that most of these factors are essential for growth and required for both in vivo polyadenylation and trans splicing, arguing for a general coupling of these two mRNA-processing reactions.
Carbon starvation survival of Listeria monocytogenes in planktonic state and in biofilm: A proteomic study

The proteomes of Listeria monocytogenes expressed in suspension and biofilm state, in the presence and absence of a caron source, were analysed by two‐dimensional electrophoresis with the help of computer software. The up‐regulated proteins in each case were identified by peptide sequencing using electrospray ionisation tandem mass spectrometry and a database search against the Listeria genome was performed. Relevant functions could be attributed to a number of the induced proteins which contibute to the understanding of the mechanisms of starvation survival of L. monocytogenes in planktonic state and in biofilm.
FAMILIAL HYPOKALAEMIA AND HYPOMAGNESAEMIA, GITELMAN'S SYNDROME, REPORT OF 6 CASES

Six cases of renal wasting of potassium and magnesium are described in four families. Four patients presented with tetany, whilst two were asymptomatic siblings of these. One patient also suffered from a progressive external ophthalmoplegia syndrome and mental retardation, whilst the remaining five were normal in physical and intellectual development. One patient (now aged 31 years) has subsequently given birth to two normal children, but maintains very low serum levels of both potassium (2.0 mM) and magnesium (0.5 mM) when off electrolyte therapy. No abnormalities in calcium metabolism were found and there was no other evidence of tubular reabsorptive defect apart from slight defects in urinary concentration and acidification. In particular sodium chloride conservation appeared normal. Plasma renin levels were normal, or slightly raised in these patients and urinary aldosterone levels were normal, distinguishing this condition from Bartter's syndrome. Magnesium loading did not affect potassium wasting, nor did potassium loading alter the renal handling of magnesium. Spironolactone was able to improve tubular reabsorption of potassium, but not of magnesium.Treatment consists of magnesium and potassium supplements to the diet. Despite continuing severe renal losses of both potassium and magnesium these patients show very few or no overt symptoms over many years, and renal function is otherwise well preserved. It is suggested that this condition be known as Gitelman's Syndrome.
Giant-Cell Anaplastic Carcinoma With Osteoclastic Giant Cells of the Chest Cavity: A Distinctive Form of Thymic Carcinoma?

Here, the authors describe a case of giant-cell anaplastic carcinoma with osteoclastic giant cells of the chest cavity—which could be a distinctive form of thymic carcinoma—which expressed CD5 and CD45. To the authors’ knowledge, there has been no previous report on this subject. A 62-year-old woman presented with continuous pain in the left back associated with coughing and shortness of breath for more than 2 months prior to referral to the hospital. Palliative resection of a mediastinal tumor was performed. During the operation, it was found that the mass occupied most of the chest invading the chest wall, aorta, vena cava, and lung tissue. The patient soon died from diabetic complications in spite of anti-infection treatment. The tumor was composed of large areas of necrosis and anaplastic neoplastic giant cells with high mitotic activity, and osteoclast-like cells; there was marked inflammatory cell infiltration. The anaplastic neoplastic giant cells were immunoreactive for CKpan, CD5, CD45, VIM, and p53. Approximately 50% to 60% of the tumor cells showed immunoreactivity for Ki-67. In situ hybridization for Epstein-Barr virus—encoded RNA was negative for tumor cells and nonneoplastic osteoclastic giant cells. Because this tumor is very rare, extensive clinical, radiological, and morphological examinations as well as immunohistochemical studies are essential to make the diagnosis.
Development of a sensitive quantitative focal assay for human immunodeficiency virus infectivity

Accurate and sensitive quantitation of infectious human immunodeficiency virus (HIV) has been difficult to achieve. In this report, a quantitative focal immunoassay (FIA) for HIV was developed using human HeLa cells rendered susceptible to HIV infection by introduction of the CD4 gene via a retrovirus vector. Infected cells were identified by using human anti-HIV antibodies or mouse monoclonal antibodies specific for HIV together with secondary fluorescein- or peroxidase-conjugated antibody specific for mouse or human immunoglobulins. The assay identified cells infected with either wild-type or culture-adapted HIV isolates and was capable of detecting 1 positive cell in 10(6) cells. The FIA was also effective at detecting cell-free HIV, and in contrast to assays using A3.01, CEM, and other human leukemia cells, the FIA detected most wild-type HIV isolates. HIV neutralization could be determined by using the FIA, and two monoclonal antibodies reactive with HIV gp120 were found to neutralize only the LAV-IIIB strain of HIV. These monoclonal antibodies, as well as antibodies in serum samples from patients with acquired immune deficiency syndrome, were able to inhibit the spread of HIV infection in human lymphocyte suspension cultures but not in CD4-positive HeLa cells growing attached to plastic dishes.
Use of the SF-36 and other health-related quality of life measures to assess persons with disabilities.

This article evaluates the appropriateness of existing approaches to the assessment of health-related quality of life (HRQOL) for persons with disabilities. We compare the conceptual model of HRQOL from the Medical Outcomes Study with the World Health Organization's International Classification of Functioning, Disability and Health. In addition, we examine the attitudes toward disease "burden" that arise from these 2 models. We note how values or the importance attached to domains of health can change with fluctuations in physical health. Further, we summarize arguments in favor of developing targeted measures of persons with disabilities. Finally, we discuss some methodologic issues in assessing physical functioning, attributions to health versus disability, and mode of administration. We conclude that users of "standard" HRQOL measures need to be aware of their limitations for assessing persons with disabilities.
Sentiment Analysis: Gauging the Effect of News on Stock Prices in Indian Stock Market

 Abstract —This paper analyses the relationship between the financial news reported in the mass media and its effects on the stock market returns. We have devised an algorithm that successfully determines polarity in financial texts using web scrapping and machine learning techniques in python to gather news texts from major financial news websites like Economic Times, Money Control, Reuters, The Hindu etc. The data collected is divided into 3 sentiments namely positive, negative and compound. By correlating the closing prices of leading players of the major sectors (like ITC-FMCG, TCS–IT, HDFC-Banking, ONGC-Energy, SunPharma-Pharmaceuticals, Maruti Suzuki-Automobile and SBI-Banking) with the sentiment generated from our algorithm we were able to conclude that indeed stock market returns are largely affected with the news and any significant news or shocks about a company can affect its stock price in the short run even though the news doesn’t have any bearing on the company’s fundamentals. Algorithms like the one used in this paper can be used to predict stock market returns in the event of any news by extrapolating the results of market behavior from the past.
Minimally Displaced Radial Head/Neck Fractures (Mason Type-I, OTA Types 21A2.2 and 21B2.1): Are We “Over Treating” Our Patients?

Objective: The purpose of this study was to investigate the nonoperative treatment strategies for Mason–Johnson type-I radial head fractures. Design and Setting: Retrospective review of every patient with a closed radial head/neck fracture who presented to our tertiary care specialty institution in the past 2 years. Patients/Participants: A search of ICD-9 code 813.05, closed fracture of the radial head/neck, in our electronic record system yielded 82 consecutive patients. Main Outcome Measurements: Complications and treatment interventions were recorded. Demographic, radiographic, and physical examination data were collected for all patients treated nonoperatively and analyzed for association with recommendation for continued follow-up and radiographic assessment. Results: Fifty-four patients (68%) had 56 nondisplaced or minimally displaced (<2 mm) radial head or neck fractures without an additional injury to the affected limb. All patients were treated nonoperatively, and no patient in this cohort developed a complication or had any medical or surgical intervention other than physical therapy. No radiographic or physical examination measure was significantly associated with recommendation for the second outpatient follow-up, third outpatient follow-up, or with the number of additional radiographs ordered beyond the initial examination. An average of 4.4 (SD, 3.3) additional x-rays were taken of each affected elbow after initial outpatient presentation. Conclusions: Orthopaedic surgeons are likely over treating patients with Mason–Johnson type-I radial head fractures by recommending frequent radiographic follow-up without modifying treatment, leading to unnecessary patient visits, radiation exposure, and increased costs. Level of Evidence: Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Patterns of Cost for Patients Dying in the Intensive Care Unit and Implications for Cost Savings of Palliative Care Interventions.

BACKGROUND Terminal intensive care unit (ICU) stays represent an important target to increase value of care. OBJECTIVE To characterize patterns of daily costs of ICU care at the end of life and, based on these patterns, examine the role for palliative care interventions in enhancing value. DESIGN Secondary analysis of an intervention study to improve quality of care for critically ill patients. SETTING/PATIENTS 572 patients who died in the ICU between 2003 and 2005 at a Level-1 trauma center. METHODS Data were linked with hospital financial records. Costs were categorized into direct fixed, direct variable, and indirect costs. Patterns of daily costs were explored using generalized estimating equations stratified by length of stay, cause of death, ICU type, and insurance status. Estimates from the literature of effects of palliative care interventions on ICU utilization were used to simulate potential cost savings under different time horizons and reimbursement models. MAIN RESULTS Mean cost for a terminal ICU stay was 39.3K ± 45.1K. Direct fixed costs represented 45% of total hospital costs, direct variable costs 20%, and indirect costs 34%. Day of admission was most expensive (mean 9.6K ± 7.6K); average cost for subsequent days was 4.8K ± 3.4K and stable over time and patient characteristics. CONCLUSIONS Terminal ICU stays display consistent cost patterns across patient characteristics. Savings can be realized with interventions that align care with patient preferences, helping to prevent unwanted ICU utilization at end of life. Cost modeling suggests that implications vary depending on time horizon and reimbursement models.
Leading transformative change collectively: an inquiry into ways of stewarding co-evolutionary 'Patterns of Aliveness' for global sustainability transformation

XIII Dutch Summary . XIV Refereed Articles and Books . XV Additional Online, Book and Article Publications . XVI Conference Presentations . XVIII Acknowledgements . XX Introduction and Context: The State of the World . 1 Chapter 1: . 11 1 The Point of Departure: The Collective Leadership Compass . 11 Chapter 2: . 23 2 Methodology and Approach . 23 Chapter 3: . 31 3 Global Transformation as a Collective Leadership Challenge. 31 3.1 The Emerging Discourse on Leadership as the Capacity of a Collective. 35 3.2 Advancing a Systems View for Leading Transformative Change Collectively . 40 Chapter 4: . 43 4 A Living Systems Perspective for Leading Transformative Change . 43 4.1 The Emergence of a Systems View of Life . 44 4.2 The Pattern Approach to Understanding Life Processes . 46 4.2. 1 Pattern Cognition as the Process of Life . 48 4.2. 2 A Pattern Approach to Socio-Ecological Resilience . 52 4.2. 3 Patterns as Life-Enhancing Design Structures . 59 4.2. 4 The Feeling of Aliveness as an Intentional Driver for Sustainability . 65 4.2. 5 Summary of Preliminary Conclusions . 71 Chapter 5: . 75 5 Organizing Principles that Enhance Aliveness in Living Systems . 75 5.1 Propositions About Essential Features of Life-Enhancing Systems . 76 5.2 The Emerging ‘Patterns of Aliveness’ Theory and the Human Realm . 84 5.3 ‘Patterns of Aliveness’ as Guidance for Leading Transformative Change . 87 5.4 The Six Aliveness Enhancing Principles . 92
Analyzing technological innovation in low and medium-low tech peruvian manufacturing companies

Greater attention should be given to low- and medium-low- tech companies in emerging countries because they contribute a significant share of GDP and employment in their economies. However, innovation practices of these companies have not taken yet the attention of innovation scholars. This article proposes a model to study how absorptive capacity, perceived impact of information sources and expenditure in acquisition of technology are associated with technological innovation practices in Peruvian low- and medium-low-tech manufacturing companies. A sample of 856 manufacturing companies of low- and medium-low- tech was obtained from the first National Survey of Innovation in the Manufacturing Industry carried out in 2012. A SEM model is proposed and has been analyzed using a partial least squares approach. Our study reveals that absorptive capacity is associated to improve technological innovation, that perceived impact of information sources are important for absorptive capacity and that greater importance should be given to the acquisition of technology: machinery, hardware and software.
Bone hydatid disease

Bone hydatid disease lacks a typical clinical appearance and image characteristics on x ray or CT are similar to those of tuberculosis, metastases and giant cell tumour or bone cysts. However, MRI does show distinctive diagnostic features of bone hydatid disease, especially in the spine. Until recently, treatment of osseous hydatid disease has been entirely surgical. Effective chemotherapy using benzimidazoles, particularly mebendazole, albendazole and combination treatments, has now been achieved in experimental studies and clinical practice. However, most of these drugs are still in the experimental stage or are in the early stages of clinical use.
National culture as a driver of pro-environmental attitudes and behavioural intentions in tourism

Abstract Voluntary changes in consumer behaviour hold significant potential to mitigate the growing environmental repercussions of tourism. Such behavioural changes can occur due to positive behavioural intentions that are in turn underpinned by pro-environmental consumer attitudes. To reinforce voluntary behavioural changes in tourism, it is paramount to understand the major drivers of pro-environmental consumer attitudes. Although national culture can influence pro-environmental attitudes of tourists, its role has been under-researched, especially in the context of emerging tourist markets. This study utilised the environment-orientated dimensions, or value orientations, of major cultural frameworks (Hofstede; Schwartz; Trompenaars & Hampden-Turner) to explore the effect of national culture on pro-environmental attitudes and behavioural intentions in a large-scale quantitative survey of Polish tourists. Correlation between the cultural background of tourists and their pro-environmental attitudes was established, thus highlighting the need for national culture to become an integral element of future scientific, policy-making, and managerial discourse on the key drivers of more sustainable consumer behaviour in tourism.
Initial evaluation of carotid angioplasty and stenting with three different cerebral protection devices.

OBJECTIVE The purpose of the study was to assess the effectiveness of cerebral protection devices during carotid artery angioplasty and stent placement. METHODS Between September 1998 and September 1999, carotid angioplasty and stenting were performed in 46 patients with symptomatic (39.1%) or asymptomatic (60.9%) severe carotid artery stenosis. Wallstents were used in all patients with selective predilatation. Cerebral protection devices were used in 25 of these patients. Primary end points were perioperative neurologic complications and mortality. Data were collected prospectively. RESULTS The overall combined end point of all neurologic deficits and death rate was 4.34%. Two neurologic events (one transient ischemic attack and one minor stroke) occurred in the unprotected group (9.53%) versus none in the group with cerebral protection. This difference is not statistically significant. The mortality rate was 0% for both groups. On an intention to treat basis, the overall technical success rate for carotid angioplasty was 97.8%, and for placement of cerebral protection devices it was 100%. An important number of particles of different sizes were captured in all cases in which cerebral protection devices were used. CONCLUSION Experience has shown that cerebral protection during carotid angioplasty and stenting is technically feasible and appears to be effective in preventing procedure-related neurologic complications. Further investigation is warranted.
A New Hybrid Multilevel Inverter Topology with Harmonics profile improvement

A new hybrid multilevel inverter topology with harmonics profile improvement is presented in this paper. From the literature, it is agreed that hybrid multilevel inverter with its DC sources configured in trinary fashion presents the largest output levels and the lowest total harmonics distortion (THD) percentage. However the output contains low order harmonics due to the impossibility of modulating all adjacent voltage levels at high frequency. With this proposed topology, all adjacent voltage levels are possible to be modulated at high frequency. As a result, the output voltage waveform presents better harmonics profile. The proposed topology and its modulation scheme are presented and verified with simulation results
The Use of Hegelian Dialectics in Nursing Science

The aim of this column is to describe dialectics as a philosophy and method which can be used by nurses to make a contribution to nursing science. Dialectics can be used in three ways: as a philosophical approach, as a method using the dialectic laws, and as a method of describing the dialectic process by focusing on the dynamic elements of the process. Dialectics can also be combined with hermeneutics.
Prime Ministers unchained? Explaining Prime Minister Policy Autonomy in coalition governments

The personalization of politics has become a central concern in political science. This is also true for parliamentary governments, where the Prime Minister has allegedly acquired an increasing relevance. Nonetheless, a key question remains unanswered: How can we estimate the Prime Minister Policy Autonomy (PMPA) in parliamentary governments? Moreover, what are the determinants of this autonomy? This article aims to answer these questions by proposing a novel and easily replicable index of PMPA, based on data from an analysis of Prime Ministers’ and members of Parliament’s parliamentary speeches, and specifically from cosine similarity analysis. In this article, we explore PMPA by focussing on two most different cases of coalition governments, Italy and Germany between 1994 and 2014. A multilevel regression analysis shows that coalition-related factors strongly influence PMPA, party-related factors are somewhat relevant, and the Prime Minister-related factor (its selectorate) does not have a significant impact on such autonomy.
“Enjoy your feeling”: A media archaeology of material publics

Ubiquitous technology depends upon imposing standards. Choices in function and form reflect the homogenization of artifacts, necessitated by the intentions of experts to satisfy a plurality of users. In material publics, users with expert knowledge can develop customized artifacts satisfying desired affordances or aesthetics. This project involves a media archaeology of computer keyboard design to explore the relationship between experts, publics, and the creation of these artifacts. Participation in these communities and study of enthusiast records result in a public-expert knowledge. The importance granted to minutia of design, from the choice of plastics to spring tensioning, parallels new form factors that reflect highly personalized choices. These reassert user control over the materiality of an otherwise ubiquitous and mundane mediating artifact. Publics then create a new political materiality by recomposing artifacts beyond what commercial expertise prescribes.
Anti-Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry Proanthocyanidins

ABSTRACT A-type cranberry proanthocyanidins (AC-PACs) have recently been reported to be beneficial for human health, especially urinary tract health. The effect of these proanthocyanidins on periodontitis, a destructive disease of tooth-supporting tissues, needs to be investigated. The purpose of this study was to investigate the effects of AC-PACs on various virulence determinants of Porphyromonas gingivalis as well as on the inflammatory response of oral epithelial cells stimulated by this periodontopathogen. We examined the effects of AC-PACs on P. gingivalis growth and biofilm formation, adherence to human oral epithelial cells and protein-coated surfaces, collagenase activity, and invasiveness. We also tested the ability of AC-PACs to modulate the P. gingivalis-induced inflammatory response by human oral epithelial cells. Our results showed that while AC-PACs neutralized all the virulence properties of P. gingivalis in a dose-dependent fashion, they did not interfere with growth. They also inhibited the secretion of interleukin-8 (IL-8) and chemokine (C-C motif) ligand 5 (CCL5) but did not affect the secretion of IL-6 by epithelial cells stimulated with P. gingivalis. This anti-inflammatory effect was associated with reduced activation of the nuclear factor-κB (NF-κB) p65 pathway. AC-PACs may be potentially valuable bioactive molecules for the development of new strategies to treat and prevent P. gingivalis-associated periodontal diseases.