Miracle-dz/UniFAR-FTUs huggingface dataset:
Interaction of the Grb10 Adapter Protein with the Raf1 and MEK1 Kinases*

Grb10 and its close homologues Grb7 and Grb14, belong to a family of adapter proteins characterized by a proline-rich region, a central PH domain, and a carboxyl-terminal Src homology 2 (SH2) domain. Their interaction with a variety of activated tyrosine kinase receptors is well documented, but their actual function remains a mystery. The Grb10 SH2 domain was isolated from a two-hybrid screen using the MEK1 kinase as a bait. We show that this unusual SH2 domain interacts, in a phosphotyrosine-independent manner, with both the Raf1 and MEK1 kinases. Mutation of the MEK1 Thr-386 residue, which is phosphorylated by mitogen-activated protein kinase in vitro, reduces binding to Grb10 in a two-hybrid assay. Interaction of Grb10 with Raf1 is constitutive, while interaction between Grb10 and MEK1 needs insulin treatment of the cells and follows mitogen-activated protein kinase activation. Random mutagenesis of the SH2 domain demonstrated that the Arg-βB5 and Asp-EF2 residues are necessary for binding to the epidermal growth factor and insulin receptors as well as to the two kinases. In addition, we show that a mutation in Ser-βB7 affects binding only to the receptors, while a mutation in Thr-βC5 abrogates binding only to MEK1. Finally, transfection of Grb10 genes with specific mutations in their SH2 domains induces apoptosis in HTC-IR and COS-7 cells. These effects can be competed by co-expression of the wild type protein, suggesting that these mutants act by sequestering necessary signaling components.
Record Single-Mode, High-Power VCSELs by Inhibition of Spatial Hole Burning

In this paper, we present fixed-wavelength, vertical-cavity-surface-emitting lasers (VCSELs) based on InP with remarkable single-mode (SM) output powers. It is shown that a precise choice of the diameters of the ring geometry of the bottom GaInAs intracavity contact layer severely affects the guiding behavior of the fundamental and first-order modes inside the cavity. Experimental data, statistics, and theories will be discussed on how to overcome spatial hole burning and how to master thermal guiding and efficient current injection in order to extend SM output powers to beyond 8 mW at room temperature for VCSELs with 7 μm aperture. Coming along with side-mode suppression ratios exceeding 50 dB and continuous electrothermal wavelength tunings exceeding 8 nm, these laser devices define a new state of the art of electrically pumped InP-based short-cavity long-wavelength VCSELs at 1.55 μm emission.
Architecture exploration of flash memory storage controller through a cycle accurate profiling

Recently, NAND flash memory has been widely adopted as a storage medium in various devices such as mobile phones, MP3 players, and digital cameras. In particular, Solid State Drives (SSDs), which are composed of multiple NAND flash memories, have gradually replaced hard disk drives (HDD). However, SSDs have an inherent weakness stemming from NAND flash memory and its complex architecture. This phenomenon makes it difficult to analyze and optimize the performance of SSD controllers. To overcome this weakness, highly accurate system simulations are needed for exploring architectural parameters to maximize the performance during the design phase. In this paper, we implement a simulator that considers all of the hardware components in SSD to assist in generating quantitatively accurate analysis when an algorithm or controller is realized. This simulator models the detailed characteristics of hardware components such as operation clock frequency and resource conflicts in order to represent SSD in great detail. In the experiments section, we verify the impacts of interface speed, page size, and other configuration parameters by using this cycle accurate simulator. These analysis results can then be used as raw data for optimization.
Words and silence: the relationship between Benedict XVI and the media

Abstract The relationship between Benedict XVI and the media could be called both tense and intense. In the following pages, I analyze four episodes especially demonstrative of this relationship: the publication of The Ratzinger Report (1985), the controversial Regensburg Address (2006), the subsequent Papal visit to Turkey and the 2010 visit to the United Kingdom–this last one ending, ironically, on an idyllic note. By examining these events, we can better understand Benedict XVI’s reflections about the media and social networks, and the necessary relationship between reason, word, silence and truth.
A Virtual Reality System for Neurobehavioral and Functional MRI Studies

We are developing a VR system of integrated software and hardware for scientific research and clinical application. The system is sufficiently flexible and broad-based in appeal that neurobehavioral researchers from a variety of disciplines might be interested in using it for basic research and clinical studies. The system runs on a standard Windows-based personal computer with a high-performance graphics card. Options allow a head-mounted display, dataglove, simultaneous physiological monitoring or use within neuroimaging machines such as magnetic resonance imaging (MRI) scanners. Currently, the software consists of a virtual world of nearly a dozen interconnected environments that the subject can freely navigate. Additional environments can be built and easily added to the application. A startup interface provides menus for selecting characters and objects that a researcher might want to put at specific locations within the simulation. Interactivity is provided for many typical objects such as doors, chairs and money. There are more than 50 characters in the world, most of them animated or interactive. All movements and actions of the subject within the world are tracked and recorded to an Excel spreadsheet for data analysis. Overlay maps are available as navigational aids. Concurrent physiological data can be acquired on up to 16 channels. The system provides synchronization of the VR simulation with physiological recordings and functional MR images. A spatial navigation memory task was performed with the integrated VR/fMRI system, and some pilot data is presented that shows robust activation in multiple cortical areas appropriate to the task.
Practical, laboratory‐scale synthesis of Nin‐formyl tryptophan hydrobromide

A range of inorganic and organoelement halides was evaluated as acidic promoters of direct Nin‐formylation of tryptophan. In addition to Me3SiBr, the less expensive PBr3 was found to be highly efficient and was selected for further optimization. A convenient and reproducible synthetic procedure for Nin‐formyltryptophan hydrobromide developed in this way was scaled to 150 mmol and successfully extended to some derivatives of Trp and closely related indoles as detailed in the present paper. The scope of the method seems to be restricted to indoles substituted at C‐3. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd.
Critical Review of Umbrella, Basket, and Platform Designs for Oncology Clinical Trials

The successful development of new drugs with a companion diagnostic based on genomic alteration of an oncogene has led to rethinking of all phases on clinical development of cancer drugs. We critically review some of the new clinical trial designs for biomarker‐based cancer drug development. We try to clarify the objectives of the new designs and examine completed trials using these designs to evaluate what has been learned about these designs.
Interaction between Protein Subunits of the Type IV Secretion System of Bartonella henselae

ABSTRACT In this study we used the yeast two-hybrid system to identify interactions between protein subunits of the virB type IV secretion system of Bartonella henselae. We report interactions between inner membrane and periplasmic proteins, the pilus polypeptide, and the core complex and a novel interaction between VirB3 and VirB5.
Insulin‐like Growth Factor II in Gynecological Cancers: A Preliminary Study

PROBLEM: We have previously reported elevated serum levels of cervical human papilloma viral proteins E6 and E7 and serum insulin‐like growth factor II (IGF‐II) in women with cervical cancer and advanced cervical intraepithelial neoplasia. As most women with cervical cancer have elevated levels of serum IGF‐II, we sought to determine whether the cervical cancer and lymph node biopsies from these women demonstrated increased production of IGF‐II and whether this elevation was also present in ovarian and endometrial cancers.
Effects of Chronic Exposure to Octylphenol on the Male Rat Reproductive System

p-tert-Octylphenol (OP) is a degradation product of alkylphenol ethoxylates. OP is an endocrine disruptor known to bind to the estrogen receptor; however, effects on males are controversial. The objective of this study was to evaluate the effects of chronic exposure to OP on male reproduction. Adult Sprague-Dawley rats were administered OP for 60 d, representing 1.5 cycles of spermatogenesis. Experimental groups included a vehicle control, and three doses of OP (25, 50, or 125 mg/kg body weight [bw]) administered daily by gavage. There was a significant decrease in body weight in the 125-mg/kg group after 60 d of treatment. Both testicular and epididymal weights and histology were not altered by treatment with OP at any of the doses administered. There were no marked differences in cauda epididymal sperm counts at any doses; however, total percent sperm motility was significantly lower in rats exposed to the intermediate dose (50 mg/kg bw). There was an increase in percent static sperm cells in all OP-treated groups, with the intermediate dose (50 mg/kg) displaying a significantly higher proportion of static cells relative to untreated controls. Caput epididymal sperm motility was unaltered by OP treatment. Gene expression profiles of testes from control and high-dose-exposed rats indicate that 14 genes were modulated by at least twofold, although these changes were not statistically significant. Taken together, results from this study indicate that OP treatment of adult rats does not appear to exert major effects on male reproductive endpoints at relevant environmental exposure doses.
Extremal Density Matrices for Qudit States

An algebraic procedure to find extremal density matrices for any Hamiltonian of a qudit system is established. The extremal density matrices for pure states provide a complete description of the system, that is, the energy spectra of the Hamiltonian and their corresponding projectors. For extremal density matrices representing mixed states, one gets mean values of the energy in between the maximum and minimum energies associated to the pure case. These extremal densities give also the corresponding mixture of eigenstates that yields the corresponding mean value of the energy. We enhance that the method can be extended to any hermitian operator.
Construct validity and temporal stability of the abridged 31-item Illness Behaviour Questionnaire

Objective: Key psychometric information was sought for three newly derived dimensions from an abridged Illness Behaviour Questionnaire (IBQ-31): Affirmation of Illness (AI), Concern for Health (CH) and General Affective State (GAS). The construct validity of these scales was examined along with their test–retest reliability and long-term stability. Design: A longitudinal, observational study was conducted with 675 participants (general community members and those with either asthma, diabetes and chronic pain or chronic fatigue syndrome) providing self-report questionnaire data at baseline, with additional information sought at three (n = 483; 71.6%) and 12 months (n = 517, 76.6%). Main outcome measures: Construct validity of the IBQ-31 was explored using well-validated psychological measures of Symptom Attributions and Symptom Experience, Cognitive Distortion of Somatic Information and Illness Likelihood. Results: In general, AI, CH and GAS shared predictable empirical overlap with related psychological indices across the five samples. Adequate three-month test–retest reliability was evident, with greater score variability over 12 months. Conclusion: The IBQ-31 comprises three theoretically relevant dimensions which demonstrate relative short- and long-term stability for individuals with diverse illness experiences. Future investigations should explore the predictive validity of AI, CH and GAS, along with the potential value of ‘cut-off’ scores for clinical use.
Fluoxetine in Social Phobia: A Double-Blind, Placebo-Controlled Pilot Study

The objective of the study was to examine the efficacy of fluoxetine in social phobia. Sixty subjects were randomly assigned to 14 weeks of double-blind therapy with either fluoxetine or placebo. Dose was fixed at 20 mg for fluoxetine during the first 8 weeks of double-blind treatment; during the final 6 weeks, the dose could be increased every two weeks by 20 mg to a maximum of 60 mg/day. An intentto-treat analysis was used. A significant change from baseline to endpoint was found for both fluoxetine and placebo on the Liebowitz Social Anxiety Scale. However, no significant difference was found between fluoxetine and placebo. The change for fluoxetine was somewhat lower than that found with other selective serotonin reuptake inhibitors, whereas the placebo response was greater. Fluoxetine failed to separate from placebo in this trial. It is unknown whether a larger dose for longer duration would have yielded separation from placebo. A higher than usual placebo response rate was found.
Automatic target recognition method for millimeter-wave body scanner

Automatic target recognition is a significant function of millimeter-wave body scanner. Based on the principle of saliency and sparse coding, this paper proposes a new method for millimeter-wave body image processing and recognition. According to the characteristics such as shape, texture and gray level of the millimeter wave image, the method realizes automatic recognition for concealed objects. A large number of experiments verify the accuracy of the method on detecting objects concealed in different position of human body.
Network Scattering Parameters

Introduction and the scattering parameters of a 1-port network the scattering parameters of a 2-port network impedance transforming properties of a 2-port network stability considerations of a 2-port network power considerations of a 2-port network generalized scattering parameters of a 2-port network the scattering parameters of an N-port network.
Older LGBT Adult Training Panels: An Opportunity to Educate About Issues Faced by the Older LGBT Community

Older lesbian, gay, bisexual, and transgender (LGBT) adults face unique issues that can impede their well-being. Although many advances have helped address these issues, there is a need for education efforts that raise awareness of service providers about these issues. This study explores evaluation data of training panels provided by older LGBT adults and the views of training participants on issues faced by the older LGBT community after attending the panels. Participants were 605 students and professionals from over 34 education and communication settings. Implications for trainings on participants and older LGBT trainers are discussed.
Inhibition of Japanese Encephalitis Virus Infection by Diethyldithiocarbamate is Independent of its Antioxidant Potential

Diethyldithiocarbamate (DDTC), a low molecular weight dithiol, has been described as an immunomodulator and modifier of diverse biological actions in human and animal models, and has also been shown to be effective in several disease conditions. Therefore, we studied the therapeutic aspect of DDTC in providing inhibition of Japanese encephalitis virus (JEV) infection. DDTC tested at various doses (10–100 μmol/kg) revealed that administration at low concentration (10 μmol/kg; i.p.) on alternate days prolonged the average survival time (AST) of mice infected with lethal dose of JEV (102 LD50, i.c.) and delayed progression of the disease. The low dose also provided >80% survival in sub-clinical (105 LD50, i.c.) JEV infection. Administration of DDTC to JEV-infected mice enhanced the inducible nitric oxide synthase (iNOS) activity in brain and level of serum tumour necrosis factor-α (TNF-α). We have recently demonstrated the production of nitric oxide (NO) via induction of iNOS activity is meditated by circulating macrophage-derived factor (MDF), which may be responsible for the delayed progression of the disease. DDTC-mediated inhibition of JEV is believed to involve the augmentation of protective role of MDF as evidenced by the observation that pretreatment with anti-MDF antibody significantly decreased the AST of mice and together with the inhibition of iNOS activity. Interestingly, DDTC alone did not stimulate iNOS and TNF-α in mock-infected normal mice. These results show that DDTC may have a possible therapeutic role during JEV infection.
Consensus and its ℒ2-gain performance of multi-agent systems with intermittent information transmissions

This article addresses the consensus problem for cooperative multiple agents with nonlinear dynamics on a fixed directed information network, where each agent can only communicate with its neighbours intermittently. A class of control algorithms is first introduced, using only intermittent relative local information. By combining tools from switching systems and Lyapunov stability theory, some sufficient conditions are established for consensus of multi-agent systems without any external disturbances under a fixed strongly connected topology. Theoretical analyses are further provided for consensus of multi-agent systems in the presence of external disturbances. It is shown that a finite ℒ2-gain performance index for the closed-loop multi-agent systems can be guaranteed if the coupling strength of the network is larger than a threshold value determined by the average communication rate and the generalised algebraic connectivity of the strongly connected topology. The results are then extended to consensus with prescribed ℒ2-gain performance with a virtual leader where the underlying topology is not necessarily strong connected or contain a directed spanning tree. Numerical simulations are finally provided to verify and visualise the theoretical analysis.
Corticospinal adaptations and strength maintenance in the immobilized arm following 3 weeks unilateral strength training

Cross‐education strength training has being shown to retain strength and muscle thickness in the immobilized contralateral limb. Corticospinal mechanisms have been proposed to underpin this phenomenon; however, no transcranial magnetic stimulation (TMS) data has yet been presented. This study used TMS to measure corticospinal responses following 3 weeks of unilateral arm training on the contralateral, immobilize arm. Participants (n = 28) were randomly divided into either immobilized strength training (Immob + train) immobilized no training (Immob) or control. Participants in the immobilized groups had their nondominant arm rested in a sling, 15 h/day for 3 weeks. The Immob + train group completed unilateral arm curl strength training, while the Immob and control groups did not undertake training. All participants were tested for corticospinal excitability, strength, and muscle thickness of both arms. Immobilization resulted in a group x time significant reduction in strength, muscle thickness and corticospinal excitability for the untrained limb of the Immob group. Conversely, no significant change in strength, muscle thickness, or corticospinal excitability occurred in the untrained limb of the Immob + train group. These results provide the first evidence of corticospinal mechanisms, assessed by TMS, underpinning the use of unilateral strength training to retain strength and muscle thickness following immobilization of the contralateral limb.
Preference-aware Fast Interest Forwarding for video streaming in information-centric VANETs

Information-Centric Networking (ICN) focuses on data dissemination instead of host communication, and it is a promising solution for video streaming in Vehicular Ad-hoc Networks (VANETs). In Information-Based VANETs, content copies can be cached by arbitrary mobile nodes in the networks. Thus, it is required to optimize the routing of content requests (referred to as Interest packets) in order to quickly locate potential content providers as soon as possible. This article proposes a Preference-aware Fast Interest Forwarding for video streaming in ICN-based VANETs (PaFF). In PaFF, each mobile user creates a Highly Preferred Content Table (HPCT) to maintain the content caching status of nodes who have similar mobility patterns and video playback behavior. Based on HPCT, a preference-aware forwarder selection mechanism is proposed to select the next hop of Interest packets in order to minimize latencies and maximize reliability. Simulation results show that PaFF achieves a considerable improvement in terms of start-up delay and cache hit ratio while incurring almost the same overhead with respect to state-of-art solutions.
Enhancing the bond of a resin-based sealer to root dentine.

AIM To evaluate whether application of a total-etch/separate adhesive layer can enhance the bond of a UDMA-based sealer to dentine. METHODOLOGY The root canals of 20 decoronated maxillary premolar teeth with two canals were prepared to size 35-45, 0.04 taper using rotary NiTi instruments. The canals of each tooth were treated with application of either a total-etch/separate adhesive or the manufacturer-recommended primer, before root filling with sealer plus matching master cone using warm vertical compaction. After setting, roots were sectioned perpendicular to the long axis to obtain 1-mm-thick slices, and the root filling was subjected to the push-out test using a plunger closely matched to canal diameter. The roots of another 15 single-rooted premolars were sectioned in a bucco-lingual direction, and the cut surfaces were ground flat for microshear bond strength testing. One-half of the specimens were coated with a separate total-etch/adhesive layer, and then, a cylinder of sealer cement 1 mm diameter × 1.5 mm high was bonded to the prepared surface of all specimens. Microshear bond strength was measured in a universal testing machine after 48 h. Data were analysed using anova and paired t-tests, with significance set at P < 0.05. RESULTS Use of a separate total-etch/adhesive markedly increased both microshear bond strength and push-out strengths compared with standard primer (P < 0.001). CONCLUSION Application of a separate adhesive layer significantly increases bond strength of UDMA-based sealers to root dentine.