Miracle-dz/UniFAR-FTUs huggingface dataset:
Recurrence‐associated gene signature optimizes recurrence‐free survival prediction of colorectal cancer

High throughput gene expression profiling has showed great promise in providing insight into molecular mechanisms. Metastasis‐related mRNAs may potentially enrich genes with the ability to predict cancer recurrence, therefore we attempted to build a recurrence‐associated gene signature to improve prognostic prediction of colorectal cancer (CRC). We identified 2848 differentially expressed mRNAs by analyzing CRC tissues with or without metastasis. For the selection of prognostic genes, a LASSO Cox regression model (least absolute shrinkage and selection operator method) was employed. Using this method, a 13‐mRNA signature was identified and then validated in two independent Gene Expression Omnibus cohorts. This classifier could successfully discriminate the high‐risk patients in discovery cohort [hazard ratio (HR) = 5.27, 95% confidence interval (CI) 2.30–12.08, P < 0.0001). Analysis in two independent cohorts yielded consistent results (GSE14333: HR = 4.55, 95% CI 2.18–9.508, P < 0.0001; GSE33113: HR = 3.26, 95% CI 2.16–9.16, P = 0.0176). Further analysis revealed that the prognostic value of this signature was independent of tumor stage, postoperative chemotherapy and somatic mutation. Receiver operating characteristic (ROC) analysis showed that the area under ROC curve of this signature was 0.8861 and 0.8157 in the discovery and validation cohort, respectively. A nomogram was constructed for clinicians, and did well in the calibration plots. Furthermore, this 13‐mRNA signature outperformed other known gene signatures, including oncotypeDX colon cancer assay. Single‐sample gene‐set enrichment analysis revealed that a group of pathways related to drug resistance, cancer metastasis and stemness were significantly enriched in the high‐risk patients. In conclusion, this 13‐mRNA signature may be a useful tool for prognostic evaluation and will facilitate personalized management of CRC patients.
Osmolarity does not affect the gastric emptying rate of oral rehydration solutions.

BACKGROUND The objective of this study was to determine the effect of either carbohydrate content or osmolarity on gastric emptying rate in normal healthy subjects. METHODS In total 12 test drinks were ingested as a single 8 mL/kg per body weight bolus on an empty stomach. Six of these drinks had a different carbohydrate content, increasing stepwise from 45 to 90 g/L, but all with the same osmolarity (330 mOsm/kg). The other six drinks all contained 60 g carbohydrate/L but differed stepwise in osmolarity because of the use of maltodextrins with a difference in chain length (243 to 374 mOsm/kg). RESULTS The results show a significant negative relation between carbohydrate content and gastric emptying in the six drinks with a uniform osmolarity but progressively increasing carbohydrate content. The six drinks, which had the same carbohydrate-energy content but different osmolarities, emptied all at the same rate from the stomach. The delivery of carbohydrate-energy per minute from the stomach to the small intestine was the same for all drinks. CONCLUSIONS From these data we conclude that the rate of gastric emptying of carbohydrate-containing solutions is triggered by the carbohydrate-energy drink content or by the delivery rate of carbohydrate-energy to the gut. Osmolarity in the range studied here had no effect.
A miniaturised integrated QCM-based electronic nose microsystem

We designed, fabricated and successfully tested an integrated miniaturised quartz crystal microbalance (QCM) based electronic nose microsystem. The microsystem is an assembly of four parts: 1. a micromachined gas-liquid interface with integrated fluid channels and electrical conductors, 2. an anisotropically conductive double-sided adhesive film, 3. a QCM crystal and 4. a polymer cap with fluid and electrical ports. The choice of the multifunctional materials and the geometric features of the four-component microsystem allow a functional integration of a QCM crystal, electrical contacts, fluidic contacts and a sample interface in a single system with minimal assembly effort, a potential for low-cost manufacturing, and a few orders of magnitude reduction in system size (12* 12*4 mm3) and weight compared to commercially available instruments. The system detected ecstasy in the 100 ng range within 30 seconds.
Current Sensorless Control for Dual Active Bridge DC–DC Converter with Estimated Load-Current Feedforward

This paper proposes a current sensorless control strategy for the dual active bridge (DAB) converter with nonlinear disturbance observer (NDO)-based load current feedforward. The proposed method can regulate both the dc-bus voltage and the current of the DAB converter without current sensors. Several performance indexes are established for the parameter design and a detailed design method is presented in this paper. When a single-phase H-bridge inverter is connected, the double-frequency oscillation from the feedback path is suppressed by proper parameter design, while the transient response of dc-bus voltage can be further improved by the NDO-based load current feedforward with a notch filter in the feedforward path. The performance of the proposed control method and the effectiveness of the parameter design are verified in a prototype by experimental results.
Torque ripple minimization in PM synchronous motors using iterative learning control

Parasitic torque pulsations exist in permanent magnet synchronous motors (PMSMs) due to nonsinusoidal flux density distribution around the air-gap, errors in current measurements, and variable magnetic reluctance of the air-gap due to stator slots. These torque pulsations vary periodically with rotor position and are reflected as speed ripple, which degrades the PMSM drive performance, particularly at low speeds. Because of the periodic nature of torque ripple, iterative learning control (ILC) is intuitively an excellent choice for torque ripple minimization. In this paper, first we propose an ILC scheme implemented in time domain to reduce periodic torque pulsations. A forgetting factor is introduced in this scheme to increase the robustness of the algorithm against disturbance. However, this limits the extent to which torque pulsations can be suppressed. In order to eliminate this limitation, a modified ILC scheme implemented in frequency domain by means of Fourier series expansion is presented. Experimental evaluations of both proposed schemes are carried out on a DSP-controlled PMSM drive platform. Test results obtained demonstrate the effectiveness of the proposed control schemes in reducing torque ripple by a factor of approximately three under various operating conditions.
Accelerated Multistress Aging of Outdoor Polymeric Insulators

Multistress aging of outdoor composite polymeric insulators continues to be a topic of interest for power transmission research community. Aging due to dry conditions alone at elevated temperatures and electric stress in the presence of UV radiation environment probably has not been explored. This paper deals with long-term accelerated multistress aging under the above conditions on full-scale 11 kV distribution class composite silicone rubber insulators. To evaluate the long-term synergistic effect of electric stress, temperature and UV radiation on insulators, they were subjected to accelerated aging in a specially designed multistress-aging chamber for 12000 hours. Chemical, physical and electrical changes due to degradation have been assessed using various techniques. It has been found that the content of low molecular weight molecules and hydrophobicity reduced significantly. Also, due to oxidation and aging there is appreciable increase in surface roughness and weight percentage of oxygen. Study is under progress and only intermediate results are presented in this paper.
Virtual Screening of Chinese Herbs with Random Forest

Random Forest, a form of multiple decision trees, has been used to screen a database of Chinese herbal constituents for potential inhibitors against several therapeutically important molecular targets. These comprise cyclic adenosine 3'-5'-monophosphate phosphodiesterases, protein kinase A, cyclooxygenases, lipoxygenases, aldose reductase, and three HIV targets-integrase, protease, and reverse transcriptase. In addition, compounds were identified which may inhibit the expression of inducible nitric oxide synthase and/or nitric oxide production in vivo. A total of 240 Chinese herbs containing 8264 compounds were screened in silico, including many used on a regular basis in traditional Chinese medicine. Active compounds were selected from another database of 2597 phytochemicals and related natural products with known target affinities and covered a wide range of structural classes. Random Forest was found to perform well, even on highly unbalanced data characteristic of ligand-based screening where the compounds to be screened are far more numerous than the number of active compounds used in training. Despite a conservative screening protocol, a wide variety of compounds from Chinese herbs were hit. Of particular interest were the relatively large number of herbs predicted to inhibit multiple targets, as well as a number which appeared to contain inhibitors of the same target from different phytochemical classes. The latter point to the possibility that individual species may make use of alternative phytochemical strategies in target inhibition. A literature search provided evidence to support 83 herb-target predictions.
High efficient AC/DC Converter For micro-hydro-power plant for DC grid

In this paper, a single-phase two-stage zero-voltage-transition zero-current-transition pulse-width-modulation power-factor-correction (ZVT-ZCT-PWM-PFC) AC/DC boost converter with active snubber cell is presented, which can be interfaced with micro-hydro power plant and can be used for telecommunication, data center, and commercial building applications. Snubber cell facilitate all semiconductor switches to undergo soft-switching for every switching period. Proposed Converter operates in continuous-conduction-mode (CCM) and uses average current control scheme for PFC. Nearly unity-power-factor (UPF) operation is achieved for wide range of load. Design and analysis of a ZVT-ZCT-PWM PFC boost converter rated at 1 kW, output voltage 400 V, operating at 100 kHz, fed from AC voltage of 230 V RMS is presented. Proposed schematic is simulated in PSIM 9.0 and simulation results are provided. An efficiency of 97.8% and power factor above 0.998 is achieved at nominal power.
A weakly supervised approach to Chinese sentiment classification using partitioned self-training

With the rapid evolution of documents on the World Wide Web which express opinions, there exists an increasing demand for developing such a sentiment analysis technique that can easily adapt to new domains with minimum supervision. This article introduces a novel weakly supervised approach for Chinese sentiment classification. The approach applies a variant of self-training algorithm on two partitions split from test dataset, and combines classification results of the two partitions into a pseudo-labelled training set and an unlabelled test set, then trains an initial classifier on the pseudo-labelled training set and adopts a standard self-learning cycle to obtain the overall classification results. Experiments on the four datasets from two domains show that our approach has competitive advantages over baseline approaches; it even outperforms the supervised approach in some of the datasets despite using no labelled documents.
Identification of Time-varying Intrinsic and Reflex Joint Stiffness

We have developed a time-varying, parallel- cascade system identification algorithm to separate joint stiffness into intrinsic and reflex components at each point in time throughout rapid movements. The components are identified using an iterative algorithm in which intrinsic and reflex dynamics are identified using separate time-varying (TV) techniques based on ensemble methods. An ensemble of input- output records having the same TV behavior is acquired and used to identify the system dynamics as impulse response functions at time increments corresponding to the sampling interval. Simulation studies showed that the time-varying, parallel-cascade algorithm performed well under realistic conditions with 99.9% VAF between simulated and predicted torque. To evaluate the performance of the algorithm under realistic conditions we applied it to an ensemble of experimental data acquired under stationary conditions. Results demonstrated that the TV estimates converged to those of the established time-invariant algorithm and allowed us to determine how variance of the TV estimates varied with the number of realizations in the ensemble
Hepatocyte targeting using pegylated asialofetuin-conjugated liposomes

Abstract Background and purpose: The hepatocyte asialoglycoprotein receptor mediates uptake of desiaylated glycoproteins by receptor-mediated endocytosis. This work explores a hepatocyte-specific targeting strategy using asialofetuin (AF) covalently coupled to pegylated liposomes. Methods: AF was conjugated to the distal end of polyethylene glycol–functionalized phospholipids. Chemical modification of AF did not interfere with its receptor interaction. AF-liposomes had a size of less than 130 nm, were judged to be monodisperse and were labelled with fluorescent organic dyes or loaded with quantum dots. Results: In vitro, binding and cellular uptake of fluorescent AF-liposomes by HepG2 hepatocellular carcinoma cells were reduced at low temperature and could be competitively inhibited by an excess of unbound AF. Hepatocyte-specific targeting and internalization of AF-liposomes in vivo was confirmed in the rat and could be competitively inhibited by co-injection of unbound AF. In contrast, non-pegylated liposomes accumulated in cells of the reticuloendothelial system such as hepatic Kupffer cells and spleen after intravenous administration. Conclusion: We conclude that the use of AF-conjugated, pegylated liposomes is a promising strategy to avoid the reticuloendothelial system and specifically target hepatocytes via the asialoglycoprotein receptor in vitro as well as in vivo.
An assessment of the uses of homologous interactions

MOTIVATION Protein-protein interactions have proved to be a valuable starting point for understanding the inner workings of the cell. Computational methodologies have been built which both predict interactions and use interaction datasets in order to predict other protein features. Such methods require gold standard positive (GSP) and negative (GSN) interaction sets. Here we examine and demonstrate the usefulness of homologous interactions in predicting good quality positive and negative interaction datasets. RESULTS We generate GSP interaction sets as subsets from experimental data using only interaction and sequence information. We can therefore produce sets for several species (many of which at present have no identified GSPs). Comprehensive error rate testing demonstrates the power of the method. We also show how the use of our datasets significantly improves the predictive power of algorithms for interaction prediction and function prediction. Furthermore, we generate GSN interaction sets for yeast and examine the use of homology along with other protein properties such as localization, expression and function. Using a novel method to assess the accuracy of a negative interaction set, we find that the best single selector for negative interactions is a lack of co-function. However, an integrated method using all the characteristics shows significant improvement over any current method for identifying GSN interactions. The nature of homologous interactions is also examined and we demonstrate that interologs are found more commonly within species than across species. CONCLUSION GSP sets built using our homologous verification method are demonstrably better than standard sets in terms of predictive ability. We can build such GSP sets for several species. When generating GSNs we show a combination of protein features and lack of homologous interactions gives the highest quality interaction sets. AVAILABILITY GSP and GSN datasets for all the studied species can be downloaded from http://www.stats. ox.ac. uk/~deane/HPIV.
Birthweight and blood pressure among children in Harare, Zimbabwe

AIM To determine whether poor uterine growth may be associated with increased blood pressure and subsequent hypertension in adulthood. METHODS A retrospective cohort study of 756 schoolchildren (mean age 6.5 years) was carried out in six low income areas in Harare city, Zimbabwe. Indices of intrauterine growth and blood pressure were assessed. RESULTS Adjusted for current weight, the children’s systolic blood pressure was inversely related to their birthweight; for each decreasing kg of birthweight, systolic blood pressure rose by 1.73 mm Hg (95% CI; 0.181 to 3.28). After adjustment for current weight, systolic blood pressure was also inversely associated with occipito-frontal circumference, but not with birth length or gestational age. Diastolic blood pressure was not associated with any of the intrauterine indices. CONCLUSION Fetal size may be inversely related to systolic blood pressure in childhood in an African population.
Minimizing procedural cost in diagnosing small bowel bleeding: comparison of a strategy based on initial capsule endoscopy versus initial double-balloon enteroscopy

Introduction Capsule endoscopy (CE) and double-balloon enteroscopy (DBE) detect small bowel bleeding with equal diagnostic yield. We aimed to detect factors that influence procedural cost of CE and DBE in diagnosing and treating small bowel bleeding, and to compare them with reimbursement. Methods A cost model analysed procedural cost for diagnostic CE versus diagnostic, unidirectional DBE (scenario 1) and CE plus directed therapeutic DBE (positive findings in CE) versus unidirectional diagnostic plus therapeutic DBE (scenario 2). The frequency of investigations per annum (p.a.) at which cost per procedure is equalized (break-even point) was determined for CE versus DBE. A retrospectively collected cohort of patients was used to validate the cost model and to compare procedural costs with reimbursement (German diagnosis-related groups, G-DRG). Results The break-even point at which cost per procedure is equalized for CE versus DBE was reached at 100 procedures p.a. in scenario 1 and 79 in scenario 2 for a rate of therapeutic enteroscopy of 14%, and 27 for a therapeutic enteroscopy rate of 30%. Personnel cost, procedure time, procedures p.a. and the rate of therapeutic enteroscopy had a major influence on procedural cost. In this patient cohort, the ‘CE-first’ and the ‘DBE-first’ strategies produced procedural costs of &U20AC;830 and &U20AC;1076 per patient to attain a diagnosis, and &U20AC;1042 versus &U20AC;1181 to achieve therapeutic enteroscopy, respectively. For this cohort, potential reimbursement was &U20AC;2320 and &U20AC;3047 for the ‘CE-first’ and the ‘DBE-first’ strategies, respectively (G-DRG). Conclusion Workflow management of CE versus DBE should consider frequency of investigations p.a. and probability for therapeutic enteroscopy to minimize procedural costs. The cost of DBE increases with less frequent or time-consuming investigations; CE is more robust with regard to these factors. From a third-party payer perspective, a strategy incorporating CE seems to minimize costs in G-DRG.
Stability of pedalling mechanics during a prolonged cycling exercise performed at different cadences

The aim of this study was to analyse the effect of pedalling rate on the pattern of mechanical torque application and on neuromuscular fatigue during prolonged cycling exercise. Eleven well-trained individuals performed three 1-h pedalling sessions, at 50 rev · min−1, 110 rev · min−1 and a freely chosen cadence, at an intensity corresponding to 65% of their maximal aerobic power. The mechanical torque applied on the right pedal was recorded for 30 s every 5 min while pedalling. Contractile and neural properties of the quadriceps and hamstring muscles were analysed before and immediately after each of the three pedalling sessions. The post-exercise reduction in knee extensors maximal voluntary contraction was significant (P < 0.01) irrespective of the cadence, but no difference was found between cadences. The use of a particular cadence did not lead to preferentially central or peripheral fatigue. An increase in cadence resulted in greater positive and negative work generated during pedalling. The mechanical pattern was not altered during the exercise, whatever the selected cadence. The present study demonstrates that despite the occurrence of neuromuscular fatigue, trained individuals maintained a stable pedalling pattern throughout an endurance cycling exercise for cadences ranging from 50 to 110 rev · min−1.
Computerised attention training for children with intellectual and developmental disabilities: a randomised controlled trial.

BACKGROUND Children with intellectual and developmental disabilities (IDD) experience heightened attention difficulties which have been linked to poorer cognitive, academic and social outcomes. Although, increasing research has focused on the potential of computerised cognitive training in reducing attention problems, limited studies have assessed whether this intervention could be utilised for those with IDD. This study aimed to assess the efficacy of a computerised attention training programme in children with IDD. METHODS In a double-blind randomised controlled trial, children (n = 76; IQ < 75) aged 4-11 years were assigned to an adaptive attention training condition or a nonadaptive control condition. Both conditions were completed at home over a 5-week period and consisted of 25 sessions, each of 20-min duration. Outcome measures (baseline, posttraining and 3-month follow-up) assessed core attention skills (selective attention, sustained attention and attentional control) and inattentive/hyperactive behaviour. RESULTS Children in the attention training condition showed greater improvement in selective attention performance compared to children in the control condition (SMD = 0.24, 95% CI 0.02, 0.45). These improvements were maintained 3 months after training had ceased (SMD = 0.26, 95% CI 0.04, 0.48). The attention training programme was not effective in promoting improvements in sustained attention, attentional control or inattentive/hyperactive behaviours. CONCLUSIONS The findings suggest that attention training may enhance some aspects of attention (selective attention) in children with IDD, but the small to medium effect sizes indicate that further refinement of the training programme is needed to promote larger, more global improvements.