Miracle-dz/UniFAR-FTUs huggingface dataset:
Epithelial cell integrin β1 is required for developmental angiogenesis in the pituitary gland

Significance During embryogenesis, a dense vascular network develops in the pituitary gland through the process of angiogenesis. In tandem, pituitary gland precursor cells differentiate into hormone-producing cells that will rely on the vasculature to carry out regulated endocrine function. Our data show that expression of the cell surface adhesion molecule, integrin β1, in the epithelial-derived precursor cells is required for development of the vasculature and coordinated terminal differentiation of endocrine cells. As a key component of the vertebrate neuroendocrine system, the pituitary gland relies on the progressive and coordinated development of distinct hormone-producing cell types and an invading vascular network. The molecular mechanisms that drive formation of the pituitary vasculature, which is necessary for regulated synthesis and secretion of hormones that maintain homeostasis, metabolism, and endocrine function, remain poorly understood. Here, we report that expression of integrin β1 in embryonic pituitary epithelial cells is required for angiogenesis in the developing mouse pituitary gland. Deletion of pituitary epithelial integrin β1 before the onset of angiogenesis resulted in failure of invading endothelial cells to recruit pericytes efficiently, whereas deletion later in embryogenesis led to decreased vascular density and lumen formation. In both cases, lack of epithelial integrin β1 was associated with a complete absence of vasculature in the pituitary gland at birth. Within pituitary epithelial cells, integrin β1 directs a large transcriptional program that includes components of the extracellular matrix and associated signaling factors that are linked to the observed non–cell-autonomous effects on angiogenesis. We conclude that epithelial integrin β1 functions as a critical and canonical regulator of developmental angiogenesis in the pituitary gland, thus providing insight into the long-standing systems biology conundrum of how vascular invasion is coordinated with tissue development.
locomotion in a consanguineous kindred the gene responsible for cerebellar hypoplasia and quadrupedal Homozygosity mapping and targeted genomic sequencing reveal Material Supplemental

References http://genome.cshlp. org/content/21/12/1995.full. html#ref-list-1 This article cites 44 articles, 15 of which can be accessed free at: License Commons Creative . http://creativecommons.org/licenses/by-nc/3.0/ described at as a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), ). After six months, it is available under http://genome.cshlp. org/site/misc/terms.xhtml for the first six months after the full-issue publication date (see This article is distributed exclusively by Cold Spring Harbor Laboratory Press
Assessing Fat Mass of Adolescent Swimmers Using Anthropometric Equations: A DXA Validation Study

ABSTRACT Purpose: The aim of the present study was to determine which of the published anthropometric equations is the most appropriate to estimate body-fat percentage (BF%) in adolescent swimmers. Method: Eighty-eight swimmers (45 boys, 43 girls) participated in this study. Following the recommendations of the International Society of the Advancement of Kinanthropometry, biceps, triceps, subscapular, iliac-crest, supraspinale, front-thigh, and medial calf skinfold thicknesses were measured. Waist, hip, midthigh, calf, relaxed arm, and arm flexed and tensed girths were also registered. BF% was measured with dual-energy X-ray absorptiometry (DXA) to obtain the reference value. Existing anthropometric equations were applied, and BF% results obtained from anthropometric equations were compared to DXA BF% results with the modified Bland-Altman. Results: The Flavel, Durnin-Rahaman-Siri, and Durnin-Rahaman-Brozek were the only equations that did not demonstrate statistically significant differences when compared with DXA. Conclusion: The present study showed that the best anthropometric equation from existing literature to estimate BF% in swimmers is that proposed by Durnin-Rahaman (independently of applying the Siri or Brozek equation).
Predictors of body fat gain in nonobese girls with a familial predisposition to obesity.

BACKGROUND Conflicting evidence exists on the causal factors underlying the development of excess adiposity in children. OBJECTIVE We determined the effect of energy expenditure (EE), muscle energetics, and physical fitness on weight and fat gain in prepubertal girls with or without a predisposition to obesity. DESIGN Normal-weight girls (n = 101) were recruited at 8 y of age according to parental body mass index. Eighty-eight girls completed the 2-y study, and the groups were as follows: LN, girls with 2 lean parents; LNOB, girls with 1 obese and 1 lean parent; and OB, girls with 2 obese parents. Measurements of weight, height, and body composition were taken 1 and 2 y after baseline. Girls underwent baseline measurements of EE by 24-h calorimetry and doubly labeled water, of muscle metabolism by (31)P nuclear magnetic resonance, and of fitness. RESULTS Fat mass (FM) and percentage body fat (%BF) differed significantly between the groups at years 1 and 2; the OB group had higher FM (P = 0.03) and %BF (P = 0.046) at year 1 and higher FM (P = 0.047) at year 2 than did the LN group. After adjustment for baseline weight, group, time, ethnicity, and Tanner stage, sleep EE, basal EE, 24-h EE, and peak oxygen uptake were negatively associated with FM and %BF (P < 0.04). After adjustment for the same variables, muscle oxidative capacity and free-living total EE were negatively and positively predictive, respectively, of changes in %BF between 8 and 10 y of age (both P = 0.04). CONCLUSIONS Nonobese girls with 2 obese parents have a significant risk of developing obesity. High free-living total EE and low muscle oxidative capacity predict high rates of fat gain.
Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells

The discovery of human induced pluripotent stem cells (hiPSCs) is a promising advancement in the field of regenerative and personalized medicine. Expression of SOX2, KLF4, OCT4 and MYC transcription factors induces the nuclear reprogramming of somatic cells into hiPSCs that share striking similarities with human embryonic stem cells (hESCs). However, several studies have demonstrated that hESCs and hiPSCs could lead to teratoma formation in vivo, thus limiting their current clinical applications. Aberrant cell cycle regulation of hESCs is linked to centrosome amplification, which may account, for their enhanced chromosomal instability (CIN), and thus increase their tumorigenicity. Significantly, the tumor suppressor p53 plays a key role as a ‘guardian of reprogramming’, safeguarding genomic integrity during hiPSC reprogramming. Nevertheless, the molecular mechanisms leading to development of CIN during reprogramming and increased tumorigenic potential of hiPSCs remains to be fully elucidated. In the present study, we analyzed CIN in hiPSCs derived from keratinocytes and established that chromosomal and mitotic aberrations were linked to centrosome amplification, Aurora-A overexpression, abrogation of p53-mediated G1/S cell cycle checkpoint and loss of Rb tumor-suppressor function. When hiPSCs were transplanted into the kidney capsules of immunocompromised mice, they developed high-grade teratomas characterized by the presence of cells that exhibited non-uniform shapes and sizes, high nuclear pleomorphism and centrosome amplification. Significantly, ex vivo cells derived from teratomas exhibited high self-renewal capacity that was linked to Aurora-A kinase activity and gave rise to lung metastasis when injected into the tail vein of immunocompromised mice. Collectively, these findings demonstrated a high risk for malignancy of hiPSCs that exhibit Aurora-A overexpression, loss of Rb function, centrosome amplification and CIN. Based on these findings, we proposed that Aurora-A-targeted therapy could represent a promising prophylactic therapeutic strategy to decrease the likelihood of CIN and development of aggressive teratomas derived from hiPSCs.
Evolutionary parallel local search for function optimization

This paper proposes a kind of evolutionary parallel local search technique (EPLS) that integrates the reproduction mechanisms from evolutionary algorithms and simplex method. The major aim is to explore the tradeoff between exploration and exploitation for optimizing multimodal functions. It has been cost-efficiently reached by means of parallel local search using simplex method. In each generation, EPLS partitions the population into a group of subpopulations, each of which consists of several individuals with adjacent space locations. EPLS independently locates multiple local optima in these disjoint neighborhoods, thus to reduce the probability of losing the global optimum. The local search in a neighborhood speeds up the convergence rate of simplex method. Recombination, adaptive Gaussian mutation and selection are incorporated into EPLS to further enhance the ability of global exploration and exploitation. The experimental observations and the extensive comparisons show that EPLS remarkably outperforms the standard evolutionary algorithms (EA) and some hybrid ones for almost all the problems tested, thus justifying the rationality and the competitive potential of EPLS for optimizing multimodal functions, especially for those with very rugged and deceptive topological structures.
Computing the T-matrix of a scattering object with multiple plane wave illuminations

Given an arbitrarily complicated object, it is often difficult to say immediately how it interacts with a specific illumination. Optically small objects, e.g., spheres, can often be modeled as electric dipoles, but which multipole moments are excited for larger particles possessing a much more complicated shape? The T-matrix answers this question, as it contains the entire information about how an object interacts with any electromagnetic illumination. Moreover, a multitude of interesting properties can be derived from the T-matrix such as the scattering cross section for a specific illumination and information about symmetries of the object. Here, we present a method to calculate the T-matrix of an arbitrary object numerically, solely by illuminating it with multiple plane waves and analyzing the scattered fields. Calculating these fields is readily done by widely available tools. The finite element method is particularly advantageous, because it is fast and efficient. We demonstrate the T-matrix calculation at four examples of relevant optical nanostructures currently at the focus of research interest. We show the advantages of the method to obtain useful information, which is hard to access when relying solely on full wave solvers.
Efficiency of low power audio amplifiers and loudspeakers

This paper looks at the load presented to audio amplifiers by real transducers. We consider the power losses in class-AB and class-D amplifier topologies, and determine that in order to predict the efficiency it is necessary to consider the amplifier/transducer combination. The ability of the class-D amplifier to recycle quadrature load current offers new ways to improve the efficiency.
Test–retest reliability, validation, and “minimal detectable change” scores for frequently reported tests of objective physical function in patients with non-dialysis chronic kidney disease

ABSTRACT Physical function is an important outcome in chronic kidney disease (CKD). We aimed to establish the reliability, validity, and the “minimal detectable change” (MDC) of several common tests used in renal rehabilitation and research. In a repeated measures design, 41 patients with CKD not requiring dialysis (stage 3b to 5) were assessed at an interval of 6 weeks. The tests were the incremental shuttle walk test (ISWT), “sit-to-stand” (STS) test, estimated 1 repetition maximum for quadriceps strength (e1RM), and VO2peak by cardiopulmonary exercise testing (CPET). Reliability was assessed using intraclass correlation coefficient and Bland–Altman analysis, and absolute reliability by standard error of measurement and MDC. The ISWT, STS-60, e1RM, and CPET had “good” to “excellent” reliability (0.973, 0.927, 0.927, and 0.866), respectively. STS-5 reliability was poor (0.676). The MDC is ISWT, 20 m; STS-5, 7.5 s; STS-60, 4 reps; e1RM, 6.4 kg; VO2peak, 2.8 ml/kg/min. There was strong correlation between the ISWT and VO2peak (r = 0.73 and 0.74). While there was poor correlation between the STS-5 and e1RM (r = 0.14 and 0.47), better correlation was seen between STS-5 and ISWT (r = 0.55 and 0.74). In conclusion, the ISWT, STS-60, e1RM, and CPET are reliable tests of function in CKD. The ISWT is a valid means of exercise capacity. The MDC can help researchers and rehabilitation professionals interpret changes following an intervention.
Patterns and predictors of folic acid supplement use among pregnant women: the Norwegian Mother and Child Cohort Study.

BACKGROUND Patterns and predictors of maternal folic acid supplement use have not been examined in large prospective studies of pregnant women. OBJECTIVE We examined the patterns and predictors of maternal folic acid supplement use from 2 mo before pregnancy through the eighth month of pregnancy. DESIGN Data from 22 500 women in the Norwegian Mother and Child Cohort Study with deliveries recorded in 2000-2003 were analyzed. RESULTS Folic acid supplement use increased from 11.8% at 2 mo before pregnancy to 46.9% at gestational month 3, but decreased to 26.0% at gestational month 8. Of 16 116 women (71.6%) who had taken folic acid supplements at some time before or during pregnancy, 72.4% had started use after becoming pregnant. Ten percent of the women had used supplements regularly from 1 mo before pregnancy throughout the first trimester. These women more frequently reported higher maternal and paternal education, planned pregnancies, infertility treatments, or chronic diseases. They were also more likely to be older, married, and nonsmokers and to have higher income and lower parity. CONCLUSIONS Most women started folic acid supplementation too late with respect to the prevention of neural tube defects. More effective intervention programs to improve periconceptional intakes of folic acid are needed and should consider both demographic and socioeconomic factors.
Relationship altered between functional T1ρ and BOLD signals in bipolar disorder

Functional neuroimaging typically relies on the blood‐oxygen‐level–dependent (BOLD) contrast, which is sensitive to the influx of oxygenated blood following neuronal activity. A new method, functional T1 relaxation in the rotating frame (fT1ρ) is thought to reflect changes in local brain metabolism, likely pH, and may more directly measure neuronal activity. These two methods were applied to study activation of the visual cortex in participants with bipolar disorder as compared to controls.
Dietary patterns related to glycemic index and load and risk of premenopausal and postmenopausal breast cancer in the Western New York Exposure and Breast Cancer Study.

BACKGROUND Reduced rank regression (RRR) has been used to identify dietary patterns that predict variation in a selected risk factor and may be useful in describing dietary exposures associated with glycemic index (GI) and glycemic load (GL). OBJECTIVE To estimate breast cancer risk, we compared the relative utility of RRR-derived dietary patterns predictive of GI and GL with those of simple GI and GL. DESIGN RRR was used to identify dietary patterns predicting GI and GL from food-frequency data obtained in the Western New York Exposure and Breast Cancer Study (1166 cases, 2105 controls). Odds ratios (ORs) and 95% CIs were estimated with unconditional logistic regression, adjusted for energy and nondietary breast cancer risk factors. RESULTS Sweets, refined grains, and salty snacks explained 34% of the variance in GI and 68% of the variance in GL. In general, breast cancer risks were not associated with GI, GL, or dietary pattern score. However, we observed a significant reduction in postmenopausal breast cancer risk with GI and GL pattern scores combined (OR: 0.68; 95% CI: 0.50, 0.93), especially in women with a body mass index (in kg/m(2)) >or=25 (OR: 0.64; 95% CI: 0.44, 0.93). Conversely, in premenopausal women, increased risks were associated with high GL pattern scores only for women with a body mass index >or=25 (OR: 2.21; 95% CI: 1.04, 4.69). CONCLUSIONS Although RRR may be useful in studies of diet and disease, our results suggest that RRR dietary patterns based on GI and GL provide similar information regarding the association between breast cancer, GI, and GL.
Current concept review: quality and process improvement in orthopedics

Multiple health care stakeholders are increasingly scrutinizing musculoskeletal care to optimize quality and cost efficiency. This has led to greater emphasis on quality and process improvement. There is a robust set of business strategies that are increasingly being applied to health care delivery. These quality and process improvement tools (QPITs) have specific applications to segments of, or the entire episode of, patient care. In the rapidly changing health care world, it will behoove all orthopedic surgeons to have an understanding of the manner in which care delivery processes can be evaluated and improved. Many of the commonly used QPITs, including checklist initiatives, standardized clinical care pathways, lean methodology, six sigma strategies, and total quality management, embrace basic principles of quality improvement. These principles include focusing on outcomes, optimizing communication among health care team members, increasing process standardization, and decreasing process variation. This review summarizes the common QPITs, including how and when they might be employed to improve care delivery.
Association of Prostate Cancer Risk Variants with TMPRSS2:ERG Status: Evidence for Distinct Molecular Subtypes

Background: Numerous genetic variants have been confirmed as prostate cancer risk factors. These variants may confer susceptibility to the development of specific molecular alterations during tumor initiation and progression. The TMPRSS2:ERG gene fusion occurs in roughly 50% of prostate cancers. Genetic risk variants may influence the development of this fusion. We sought to determine whether prostate cancer risk variants are differentially associated with TMPRSS2:ERG fusion–positive and negative cancer. Methods: In the Health Professionals Follow-up Study and Physicians' Health Study Tumor Cohort, we evaluated the associations of 39 prostate cancer risk SNPs with TMPRSS2:ERG fusion status, measured by ERG protein expression. Logistic regression was performed to generate OR and 95% confidence intervals. The primary outcome was ERG+ (n = 227) versus ERG− (n = 260) prostate cancer. A secondary outcome was ERG+ or ERG− cancer versus controls without cancer. Results: Six of 39 SNPs were significantly associated (P < 0.05) with ERG+ versus ERG− disease. Three SNPs were exclusively associated with the risk of ERG+, one with risk of ERG−, and two with associations trending in opposite directions for ERG+ and ERG−. Only two significant SNPs would be expected by chance. Conclusions: Prostate cancer genetic risk variants are differentially associated with the development of ERG+ and ERG− prostate cancer. Impact: Our findings suggest the molecular process of prostate carcinogenesis may be distinct for men with different underlying genetic predisposition. When examining risk factors for prostate cancer, the integration of molecular subtypes may enhance understanding of the etiology of this disease. Cancer Epidemiol Biomarkers Prev; 25(5); 745–9. ©2016 AACR.
Dynamics of microbubbles for diagnostic ultrasound imaging

Various kinds of ultrasound contrast agents have been developed for diagnostic imaging purposes and these agents have been used for therapeutic purposes. These are microbubbles, which consist of air or higher molecular weight gases, stabilized by shells of different materials, such as lipid, polymer and surfactants. In this study, the oscillations of several kinds of microbubbles with different gas cores and the role of the surrounding shell are numerically investigated with more accurate models which determine the interior gas motion and its associated thermal phenomena.
Mechanotransduction activates canonical Wnt/β-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves

In this study, Cha et al. show that the Wnt/β-catenin signaling pathway is the link between fluid flow and lymphatic vascular morphogenesis. They provide a molecular and structural framework to study mammalian lymphatic vasculature by demonstrating that mechanical stimulation is a critical regulator of lymphatic vascular development via activation of Wnt/β-catenin signaling.
Association between MMP2-1306 C/T polymorphism and prostate cancer susceptibility: a meta-analysis based on 3906 subjects

Numerous investigations have addressed the correlation between MMP2-1306C/T polymorphism and prostate cancer (PCa) susceptibility. However, these conclusions were controversial. Thus, we conducted this current meta-analysis based on six studies from PubMed, Embase, Cochrane Library, China Biology Medicine disc (CBM), China National Knowledge Infrastructure (CNKI) up to October 21st, 2016. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to evaluate the strength of the correlations. Additionally, different subgroup analyses and publication bias tests were performed. Eventually, six previous investigations consisted of 1920 cases and 1986 controls were identified and involved in this meta-analysis. Consequently, our evidence indicates a certain association between MMP2-1306C/T polymorphism and PCa risk among overall population (T vs C: OR = 1.12, 95% CI = 1.00-1.24, P = 0.040; TT+CT vs CC: OR = 1.16, 95% CI = 1.02-1.32, P = 0.026; respectively), as well as the subgroups of Asian population (T vs C: OR=1.48, 95% CI=1.13-1.94, P=0.004; TT+CT vs CC: OR = 1.66, 95% CI = 1.21-2.28, P = 0.002; respectively) and PCR-RFLP genotyped method (T vs C: OR = 1.58, 95% CI = 1.19-2.10, P = 0.001; TT+CT vs CC: OR = 1.71, 95% CI = 1.23-2.38, P = 0.001; respectively). However, no association was detected in MMP2-1306C/T polymorphism with Gleason grading or pathological stage of PCa. Our study indicates MMP2-1306 C/T polymorphism might increase PCa risk, particularly for Asian population. However, future studies comprising large cohort size from multicenter are required to confirm our conclusions.