Miracle-dz/UniFAR-FTUs huggingface dataset:
The Chromosomally Encoded Cation Diffusion Facilitator Proteins DmeF and FieF from Wautersia metallidurans CH34 Are Transporters of Broad Metal Specificity

ABSTRACT Genomic sequencing of the β-proteobacterium Wautersia (previously Ralstonia) metallidurans CH34 revealed the presence of three genes encoding proteins of the cation diffusion facilitator (CDF) family. One, CzcD, was previously found to be part of the high-level metal resistance system Czc that mediates the efflux of Co(II), Zn(II), and Cd(II) ions catalyzed by the CzcCBA cation-proton antiporter. The second CDF protein, FieF, is probably mainly a ferrous iron detoxifying protein but also mediated some resistance against other divalent metal cations such as Zn(II), Co(II), Cd(II), and Ni(II) in W. metallidurans or Escherichia coli. The third CDF protein, DmeF, showed the same substrate spectrum as FieF, but with different preferences. DmeF plays the central role in cobalt homeostasis in W. metallidurans, and a disruption of dmeF rendered the high-level metal cation resistance systems Czc and Cnr ineffective against Co(II). This is evidence for the periplasmic detoxification of substrates by RND transporters of the heavy metal efflux family subgroup.
An adapted measure of ethical climate in organisations – a South African study

A study was conducted to analyse the ethical climate typology of the Ethical Climate Questionnaire empirically, in order to develop a unique South African typology. This typology was tested for the equivalence of the construct between the private and public sector. A three ethical climate type solution was found (in contrast with the initial nine, and later five type typology). The results suggest that the construct is equivalent for both the private and public sectors. The findings could be used as a foundation for future studies, as well as for ethical climate measurement within the South African context.
Synergistic Effects of a Smac Mimetic with Doxorubicin Against Human Osteosarcoma.

BACKGROUND/AIM Second mitochondria-derived activator of caspase (Smac) is a proapoptogenic mitochondrial protein that antagonizes inhibitors of apoptosis proteins (IAPs), resulting in induction of apoptosis. In the present study we investigated the effects of a Smac mimetic in combination with doxorubicin against osteosarcoma. MATERIALS AND METHODS In vitro effects of the combination of a Smac mimetic AT-406 and doxorubicin on cell proliferation and apoptosis in osteosarcoma cell lines were examined using cell proliferation assays, flow cytometry, and immunoblot analyses. For in vivo experiments, human osteosarcoma xenografts were treated with combination of the two substances, and tumor volume and apoptotic activity in treated tumors were assessed. RESULTS In vitro studies revealed that combination of the two substances significantly inhibited osteosarcoma proliferation with decreased cIAP1 expression and induced apoptosis in osteosarcoma cells. Combination of the two substances significantly suppressed osteosarcoma growth in vivo. Moreover, decreased cIAP1 expression and increased apoptotic activity were observed in tumors treated by their combination of the substances. CONCLUSION The Smac mimetic AT-406 showed an apoptotic effect and a synergistic antitumor effect with doxorubicin on osteosarcoma. The combination of AT-406 and doxorubicin may serve as a novel therapeutic strategy for osteosarcoma treatment.
Compendium of Election Laws, Practices and Cases of Selected Commonwealth Countries

The first volume presents the constitutional provisions and election laws of a number of selected Commonwealth countries in a user-friendly way, aiming to stimulate wider discussions about the available options. In order to make comparative analyses easier, wherever possible each legislative scheme is presented in a common format, highlighting those features which are considered to be a part of the essential framework of that scheme. This compendium is not a mere collection of election laws. The form of the presentation of each legislative scheme (which at times takes account of procedures and practice guidelines) focuses on the organisation and conduct of elections for the National Assembly (by whatever name called in the country concerned). In a few cases presidential elections and elections to the Upper House are presented where the procedures therefore are considered appropriate for noting.
MRI to assess arrhythmia and cardiomyopathies

Cardiovascular magnetic resonance (CMR) is highly precise for morphological and functional analyses of the myocardium, and has been used to assess different types of cardiomyopathies. Its ability to characterize tissue, especially with gadolinium (Gd) delayed‐enhancement techniques, has shown promising results for the diagnosis of arrhythmogenic cardiomyopathies. In this review we discuss the background and potential of this approach, as well as its usefulness for assessing arrhythmias and cardiomyopathies. J. Magn. Reson. Imaging 2006. © 2006 Wiley‐Liss, Inc.
The Greeks of the Piterbarg option pricing framework

The Greeks give the sensitivity of the price of an options to a change in underlying parameters on which the value of the option depends. They are also called the risk sensitivities, risk measures or hedge parameters. Therefore Greeks provide information with regards to hedging an option. The different Greeks that are used, measure different dimensions in the risk to an option position. In practice, appropriate management of the different Greeks provide acceptable risk levels.
Biologic Characterization of H4, H6, and H9 Type Low Pathogenicity Avian Influenza Viruses from Wild Birds in Chickens and Turkeys

Abstract The pathogenesis, virus shedding, and serologic response in specific-pathogen-free (SPF) chickens and commercial turkeys against H4, H6, and H9 type low pathogenic avian influenza viruses (LPAI) from wild birds was examined. Four-week-old chickens and three-week-old turkeys were given 1 × 106 EID50 of LPAI per bird, intrachoanally, and examined for clinical signs for 3 wk. Oropharyngeal and cloacal swabs, and fecal samples, were collected at 2, 4, and 7 days postinoculation (PI) for virus detection by real-time RT-PCR. Serum was collected at 7, 14, and 21 days PI and examined for antibodies against avian influenza virus (AIV) by the enzyme-linked immunosorbant assay (ELISA) and hemagglutination inhibition tests. Tissue samples for histopathology were collected from three birds per group at 3 days PI. The hemagglutinin genes of the viruses were sequenced, and phylogenetic analysis was conducted. Clinical signs ranged from no clinical signs to moderate depression, decreased activity, and decreased food and water consumption. Based on virus detection results, SPF chickens were generally found to be shedding more virus from both the oropharynx and cloaca than were commercial turkeys. Microscopic lesion results in both species showed the predominance of lesions in the respiratory and gastrointestinal tract, which is consistent with the fact that these viruses are of low pathogenicity. In chickens and turkeys, oropharyngeal shedding strongly correlated with the lesions found in the upper respiratory tract. Turkeys had fewer lesions in the respiratory tract and more lesions in the gastrointestinal tract compared to chickens. Thirteen LPAI viruses caused seroconversion in commercial turkeys, whereas only 6 LPAI viruses caused seroconversion in SPF chickens. Phylogenetic analysis of the HA genes showed that the H4, H6, and H9 viruses evaluated here represented the full genetic diversity of North American AIVs of their respective subtypes. This data is important for surveillance and control because some of the LPAI viruses (of wild bird origin and examined in this study) that can infect and be shed by chickens and turkeys would be difficult to detect in commercial poultry. Specifically, detection is difficult because these viruses did not cause overt clinical disease or mortality, but only induced mild microscopic lesions and exhibited poor seroconversion.
The Relationship between Self-Perceived Health and Labour Market Outcomes in Romania

The influence of health status on labour market behaviour is a subject which has benefited of a wide empirical work so far. And the common finding of positive effects of a good health on labour market participation, productivity and wages has been supported by strong evidence. On the other hand, the inverse relationship the effect of labour market participation on health status -has been less studied. Moreover, the issue has benefited of little attention in Romania. Our paper explores the bidirectional relationship between self-perceived health and labour market outcomes in Romania using a simultaneous equations model. We use EU-SILC data for Romania, the sample being representative for the whole population and consisting of approximately 17 thousand of individuals aged 16 and more. We find that health has a positive influence on labour market participation and incomes, but labour market participation has an adverse effect on health. The results show that individuals do not underreport their health status to justify non-participation. In conclusion, health is endogenous to labour market participation and should be treated as so, otherwise the effects of health on labour market status could be underestimated.
Interdependent policy instrument preferences: a two-mode network approach

Abstract In policymaking, actors are likely to take the preferences of others into account when strategically positioning themselves. However, there is a lack of research that conceives of policy preferences as an interdependent system. In order to analyse interdependencies, we link actors to their policy preferences in water protection, which results in an actor-instrument network. As actors exhibit multiple preferences, a complex two-mode network between actors and policies emerges. We analyse whether actors exhibit interdependent preference profiles given shared policy objectives or social interactions among them. By fitting an exponential random graph model to the actor-instrument network, we find considerable clustering, meaning that actors tend to exhibit preferences for multiple policy instruments in common. Actors tend to exhibit interdependent policy preferences when they are interconnected, that is, they collaborate with each other. By contrast, actors are less likely to share policy preferences when a conflict line divides them.
Association between delirium resolution and functional recovery among newly admitted postacute facility patients.

BACKGROUND Delirium is common among hospitalized elders and may persist for months. The adverse impact of delirium on independence may increasingly occur in the postacute care (PAC) setting. The purpose of this study is to examine the association between delirium resolution and functional recovery in skilled nursing facilities specializing in PAC. METHODS Patients were screened for delirium on admission after an acute hospitalization at PAC facilities. Only patients with "Confusion Assessment Method"-defined delirium were enrolled. Delirium and activities of daily living were assessed prehospital, at PAC admission, and at four (2-week, and 1-, 3-, and 6-month) follow-up assessments to measure functional ability. Four distinct delirium resolution groups were created ranging from resolution within 2 weeks without recurrence to no resolution over 6 months. Repeated-measures analysis of covariance was used to determine if functional performance differed over time by delirium resolution status. RESULTS Among the 393 PAC patients, functional recovery differed significantly (p <.0001) by delirium resolution status. Patients who resolved their delirium by 2 weeks without recurrence regained 100% of their prehospital functional level, whereas patients who never resolved their delirium retained less than 50% of their prehospital functional level. Patients with slower resolving delirium and recurrent delirium had intermediate functional outcomes. CONCLUSIONS Resolution of delirium among PAC patients appears to be a prerequisite for functional recovery. Delirium resolution within 2 weeks without recurrence is associated with excellent functional recovery. Effective strategies to resolve delirium promptly and prevent its recurrence in the PAC setting will likely benefit patient rehabilitation and functional recovery.
Barriers to School-Based Parent Involvement While Living in Public Housing: A Mother’s Perspective

Parent involvement is associated with child academic outcomes, positive behaviors, and social skills. This qualitative study explored school-based parent involvement barriers experienced by nine low-income mothers. In-depth interviews were used to collect data from mothers participating in a community-based program offered in a large public housing neighborhood. Findings included three main barriers: (a) cultural and language differences in their children’s school, (b) undertones of racism from teachers and parents, and (c) being the primary caregiver or sole provider for their children. Although all parents experience challenges to school involvement, low-income mothers face additional obstacles preventing them from engaging in their children’s schools. This perceived lack of school involvement can lead to feelings of helplessness, shame, and stigma.
Reduced coagulation at high altitude identified by thromboelastography

Summary The impact of hypoxaemia on blood coagulation remains unclear despite use of a variety of measures to address the issue. We report the first use of thromboelastography (TEG) at high altitude to describe the dynamics of clot formation in whole blood samples. Seventeen healthy volunteers ascended to 5,300 m following an identical ascent profile; TEG measurements at 4,250 m and 5,300 m were compared with those from sea level. Peripheral oxygen saturation (SpO2) and haematocrit were also measured. Ascent resulted in a decline in SpO2 from 97.8 (± 1.2) % at sea level to 86.9 (± 3.3) % at 4,250 m and 79.5 (± 5.8) % at 5,300 m (p<0.001); haematocrit rose from 43.7 (± 2.8) % at sea level, to 46.7 (± 3.9) % and 52.6 (± 3.2) % at 4,250 m and 5,300 m, respectively (p<0.01). TEG reaction (R)-time and kinetic (K)-time were both increased at 5,300 m compared to sea level, 8.95 (± 1.37) minutes (min) to 11.69 (± 2.91) min (p=0.016) and 2.40 (± 0.66) min to 4.99 (± 1.67) min (p<0.001), respectively. Additionally the alpha (α)- angle was decreased from 57.7 (± 8.2) to 51.6 (± 6.4) (p<0.001). There was no change in maximum amplitude (MA) on ascent to altitude. These changes are consistent with an overall pattern of slowed coagulation at high altitude.
Mutations of polyomavirus VP1 allow in vitro growth in undifferentiated cells and modify in vivo tissue replication specificity

Mutants of polyomavirus with altered host specificities were isolated in undifferentiated C2 myoblast cells (L. Ricci, R. Maione, C. Passananti, A. Felsani, and P. Amati, J. Virol. 66:7153-7158, 1992). The mutations responsible for this phenotype belonged to either of the two classes: a large duplication of the enhancer region or a 6-bp deletion in the VP1 coding region. Since both classes of mutations enabled the virus to grow in undifferentiated myoblast cells, we investigated their ability to replicate in embryonal carcinoma cells and in various tissues in newborn mice. Our results show that both kinds of mutations confer the ability to replicate in vitro in embryonal carcinoma F9 cells; the VP1 mutants acquired an in vivo host range of replication which is different from that of their original wild-type strain, whereas the mutation in the regulatory region did not alter the in vivo growth spectrum.
Analysis of coupled-inductor configuration for an interleaved high step-up converter

High step-up converters are widely used in sustainable energy systems and recently used in automotive applications due to their high voltage gain capability. Nevertheless, with the purpose of obtaining a higher voltage gain, in comparison with conventional boost converters, current high step-up converters often employ additional multiplier cells, which may lead to significant cost-up and low power density. Therefore, a novel two-phase interleaved high step-up converter is proposed in order to minimize additional circuit volume used to achieve large voltage gain. The proposed converter addresses the purpose by a particular coupled inductor where three windings are installed in one or two cores. As a result, the proposed converter can achieve higher voltage gain than the conventional topologies by adding a winding and two diodes to the interleaved two phase boost chopper, besides the coupled-inductor configuration. This paper evaluates two arrangements of the coupled-inductor configuration of the proposed high step-up converter: 1. Three windings integrated in only one core and 2. Two independent inductors with a shared winding. The result revealed that the proposed converter shows higher voltage gain than the normal boost converter and the magnetic integration in the coupled-inductor configuration further increases the voltage gain by 20%.
The mediating role of innovative work behavior on the relationship between knowledge sharing behavior and innovation capability in Thailand private university

Amongst today turbulent business environment, innovation is considered as a main source of an organization competitive advantage. In higher education environment, especially in the private university, innovation has also received considerable concern as a major contributor in securing sustainable competitive edge in the marketplace. As a result, a number of factors have been introduced to enhance the organizational innovation capability. This research aims to examine the relationship between employees' knowledge sharing behavior, innovative work behavior and organizational innovation capability. The research surveyed a sample of 367 private university staffs in Thailand. Structural equation modeling was used to test the measurement model and path analysis was used to examine the comparative strength of direct and indirect relationships among variables. The analytical results revealed that knowledge sharing behavior showed a positive link to both innovation capability and innovative work behavior. In addition, innovative work behavior was found to be a partial mediator of the relationship between knowledge sharing behavior and innovation capability. This study provides some useful information for university administrators, and highlights management issues involving knowledge sharing behavior and understanding the influence of innovative work behavior on organizational innovation capability.
Existential behavioral therapy for informal caregivers of palliative patients: Barriers and promoters of support utilization

Abstract Objective: Several interventions have been developed during recent years to support informal caregivers of palliative patients. However, these trials reported low enrollment rates. Employing a newly developed group intervention, existential behavioral therapy (EBT), one study reported that only 13.6% of approached informal caregivers participated. The purpose of our present study was to identify the reasons for this low enrollment rate in order to improve future support designs. Method: All participants in the EBT trial (intervention vs. standard-care control group) as well as those who declined participation during a 4-month recruitment period were studied prospectively over 12 months. Andersen's behavioral model of healthcare service use was employed to identify group differences between acceptors and decliners: predisposing (age, gender, education, family status, relationship), enabling (social support, distance to hospital, caring vs. bereaved), and need factors (psychological distress, quality of life) were evaluated in a binary-logistic model. Results: Some 94 decliners were compared to 160 EBT participants (n = 81 intervention, n = 79 control). Caregivers who took part were significantly more distressed and suffered from a lower quality of life compared to decliners. Not only these need factors but also predisposing (age <55 years) and enabling (use of social/professional support, familiarity with caregiving institution) factors were associated with EBT utilization. At the 12-month follow-up, EBT intervention participants reported greater quality of life improvements than decliners or controls (p = 0.05). While all groups had mean anxiety scores below the cutoff at 12-month follow-up, decliners showed better improvement in anxiety compared to EBT participants (intervention p = 0.04, controls p = 0.03). Significance of results: On average, decliners are less burdened: they may be more resilient, may have better coping strategies, or already have a sufficient support network in place. Screening caregivers with regard to their experienced quality of life and targeting those in need, especially younger caregivers with low levels of quality of life, may help to allocate resources more appropriately.
A GMM Skewness and Kurtosis Ratio Test for Higher Moment Dependence

This article extends the variance ratio test of Lo and MacKinlay (1988) to tests of skewness and kurtosis ratios using the generalized methods of moments. In particular, overlapping observations are used in which dependencies are explicitly modeled to make the tests more powerful and have better size properties. The proposed higher-order ratio tests can be useful in risk management where risk models are estimated using daily data but multiperiod forecasts of tail risks are required for the determination of risk capital. Application of the tests finds significant higher moment dependence in the U.S. stock market returns.
A simple chemical method for the oxidation of beta-hydroxybutyrate; application of the method for the estimation of ketone bodies.

A simple one-step method employing potassium persulphate as an oxidising agent in presence of catalyst, Ag2+, for the oxidation of beta-hydroxybutyrate (beta-OHB) to acetoacetate (AcAc) has been developed and standardized. Under the condition of assay, beta-OHB (0.079-0.395 microM) was quantitatively transformed to AcAc. The reaction linearity was observed from 0.079 to 0.634 microM. Optimum conditions were: pH, 6.2; temp. , 40 degrees C; persulphate saturation, 40% and catalyst, 1.82 mM. Under the experimental condition, no reversal of inhibition caused by chloride (22.96 mM) was observed at Ag+ concentrations (9.09 and 18.18 mM), while higher conc. of Ag+ (27.27 mM) caused significant reversal of inhibition (about 60%). The maximum reversal of inhibition was achieved at Ag+ (36.36 mM). The level of ketone bodies, when estimated by the present method, was greatly enhanced during starvation period and about 2- and 12-fold higher level of ketone bodies was observed (compared to control) in rats fasted for 24 and 48 hr respectively.
Spacetime processing metasurfaces: GSTC synthesis and prospective applications

The paper presents the general concept of spacetime processing metasurfaces, synthesized by generalized sheet transition conditions (GSTCs). It is shown that such metasurfaces can perform multiple simultaneous spatio-temporal processing transformations on incident electromagnetic waves. A time-reversal space-generalized-refraction metasurface and a multi-time-space-differentiating metasurfaces are presented as applications of the general spacetime processing metasurface concept.