Miracle-dz/UniFAR-FTUs huggingface dataset:
1H NMR Investigation of the Solution Structure of Substrate-free Human Heme Oxygenase

1H NMR was used to investigate the molecular structure, and dynamic properties of soluble, recombinant, substrate-free human heme oxygenase (apohHO) on a comparative basis with similar studies on the substrate complex. Limited but crucial sequence-specific assignments identify five conserved secondary structural elements, and the detection of highly characteristic dipolar or H-bond interactions among these elements together with insignificant chemical shift differences confirm a strongly conserved folding topology of helices C–H relative to that of substrate complexes in either solution or the crystal. The correction of the chemical shifts for paramagnetic and porphyrin ring current influences in the paramagnetic substrate complex reveals that the strength of all but one of the numerous relatively robust H-bonds are conserved in apohHO, and similar ordered water molecules are located near these H-bond donors as observed in the substrate complexes. The unique and significant weakening of the Tyr58 OH hydrogen bond to the catalytically critical Asp140 carboxylate in apohHO is suggested to arise from the removal of the axial H-bond acceptor ligand rather than the loss of substrate. The interhelical positions of the conserved strong H-bonds argue for a structural role in maintaining a conserved structure for helices C–H upon loss of substrate. While the structure and H-bond network are largely conserved upon loss of substrate, the variably increased rate of NH lability dictates a significant loss of dynamic stability in the conserved structure, particularly near the distal helix F.
A Novel Compact Dual Band Reconfigurable Power Divider for Smart Antenna Systems

This paper presents a novel compact dual band reconfigurable power divider for smart antenna systems. The circuit employs two cascaded dual band hybrid couplers connected through two dual band discontinuities. The dual band discontinuities, consisting of a diplexer and two varactor diodes, can be tuned through two different biasing voltages thus allowing an independent and arbitrary choice of the power ratio in the two frequency bands. A prototype has been designed to operate in the dual band WLAN frequency range, and fabricated in microstrip technology on a 25 mils CER10 substrate. Measured scattering parameters exhibit good performance in terms of return loss, insertion loss and isolation in both WLAN bands, confirming the feasibility of the proposed architecture. The compactness of the circuit makes it suitable for employment in array configurations.
Current Waveforms for Neural Stimulation-Charge Delivery With Reduced Maximum Electrode Voltage

This paper contains results on the design of electrical signals for delivering charge through electrodes to achieve neural stimulation while reducing the peak electrode voltage. A generalization of the usual constant current stimulation phase to a stepped current waveform is presented. Techniques based on optimization and linear dynamic system theory are then applied to design the magnitude of each current segment in such a way as to minimize the maximum electrode voltage, while transferring a designated quantity of charge in a specified time. Experimental results are provided which validate the approach in saline and in neural tissue.
Quetiapine for hypersexuality and delusional jealousy after stroke.

To the Editors: Hypersexuality and psychotic symptoms are occasionally associated with Parkinson’s disease, dopamine agonist therapy for Parkinson’s disease, dementia, and temporal lobe seizures, but rarely with stroke. Although some studies have shown that quetiapine is effective in treating psychosis and motor functions in Parkinson’s disease, as far as we know, there have not been any treatment response studies in the area of hypersexuality and psychosis after a stroke. We present a case in which hypersexuality and delusional jealousy after a stroke were successfully managed with quetiapine.
Multilocus sequences confirm the close genetic relationship of four phytoplasmas of peanut witches'‐broom group 16SrII‐A

Four witches'‐broom diseases associated with Arachis hypogaea (peanut), Crotalaria pallida, Tephrosia purpurea, and Cleome viscosa were observed in Hainan Province, China during field surveys in 2004, 2005, and 2007. In previously reported studies, we identified these four phytoplasmas as members of subgroup 16SrII‐A, and discovered that their 16S rRNA gene sequences were 99.9–100% identical to one another. In this study, we performed extensive phylogenetic analyses to elucidate relationships among them. We analyzed sequences of the 16S rRNA gene and rplV–rpsC, rpoB, gyrB, dnaK, dnaJ, recA, and secY combined sequence data from two strains each of the four phytoplasmas from Hainan province, as well as strains of peanut witches'‐broom from Taiwan (PnWB‐TW), “Candidatus Phytoplasma australiense”, “Ca. Phytoplasma mali AT”, aster yellows witches'‐broom phytoplasma AYWB, and onion yellows phytoplasma OY‐M. In the 16S rRNA phylogenetic tree, the eight Hainan strains form a clade with PnWB‐TW. Analysis of the seven concatenated gene regions indicated that the four phytoplasmas collected from Hainan province cluster most closely with one another, but are closely related to PnWB‐TW. The results of field survey and phylogenetic analysis indicated that Cr. pallida, T. purpurea, and Cl. viscosa may be natural plant hosts of peanut witches'‐broom phytoplasma.
Research on the definition and model of software testing quality

Aim of software testing is to find out software defects and evaluate the software quality. In order to explain the software testing can really elevate the software quality, it is necessary to assess the quality of software testing itself. This paper discusses the definition of software testing quality, and further builds the framework model of software testing quality (STQFM) and the metrics model of software testing quality (SFTMM). The availability of the models is verified by example application.
Mixed models for ordinal data: A pharmacokinetic study on the effectiveness of drug for the reduction of epileptic seizures

Population pharmacokinetics (PK)/pharmacodynamics analysis is considered as an important component of drug development process in these days. Owing to different standardization techniques in different laboratories, the drug concentrations in blood are reported in intervals in which they lie. For obvious reason the outcomes are then recorded as multiple discrete indicators with natural ordering. But in view of the reduced amount of information, the modeling of such data becomes intrinsically more difficult than that of continuous data. The primary concern in this study is to analyze a one‐compartmental PK model for ordinal data that can be used to describe the change in the concentration of drug over time. A flexible Monte Carlo EM‐based two‐step estimation method is proposed. Such a method is, in general, capable of analyzing a reasonably wide class of nonlinear mixed models. The modeling strategy is applied to a Phase I study of the drug Divalproex and its metabolite, developed for the treatment of epilepsy. The model's performance is evaluated through simulation. Copyright © 2008 John Wiley & Sons, Ltd.
Subject-to-subject adaptation to reduce calibration time in motor imagery-based brain-computer interface

In order to enhance the usability of a motor imagery-based brain-computer interface (BCI), it is highly desirable to reduce the calibration time. Due to inter-subject variability, typically a new subject has to undergo a 20-30 minutes calibration session to collect sufficient data for training a BCI model based on his/her brain patterns. This paper proposes a new subject-to-subject adaptation algorithm to reliably reduce the calibration time of a new subject to only 3-4 minutes. To reduce the calibration time, unlike several past studies, the proposed algorithm does not require a large pool of historic sessions. In the proposed algorithm, using only a few trials from the new subject, first, the new subject's data is adapted to each available historic session separately. This is done by a linear transformation minimizing the distribution difference between the two groups of EEG data. Thereafter, among the available historic sessions, the one matched the most to the new subject's adapted data is selected as the calibration session. Consequently, the previously trained model based on the selected historic session is entirely used for the classification of the new subject's data after adaptation. The proposed algorithm is evaluated on a publicly available dataset with 9 subjects. For each subject, the calibration session is selected only from the calibration sessions of the eight other subjects. The experimental results showed that our proposed algorithm not only reduced the calibration time by 85%, but also performed on average only 1.7% less accurate than the subject-dependent calibration results.
Some Issues on Recent Monetary Policy in China

The relative active monetary policy in China has had an effect on guarding against financial venture and improving the quality of financial assets in terms of the aim of policy.And it is also producing an effect in terms of time lag,so it is a lopsided view that China’s economy has been fallen into Keynes’s Liquidity trap.To ensure the macro economy increasing continuously,the moderate inflation policy at the rate of 3 to 6 percent should be the medium term and long objective of macro economic policy in China.At present,one of the main financial task is to perfect the transmissible mechanism of monetary policy.
Attracting tourists to travel companies’ websites: the structural relationship between website brand, personal value, shopping experience, perceived risk and purchase intention

This article aims to examine the structural relationship between website brand, personal value, shopping experience, perceived risk and purchase intention from travel websites. Built upon the theory of consumers’ perceived risk, a theoretical model was proposed and a questionnaire was developed. The fieldwork utilized responses from 409 participants who purchased travel items from websites of Malaysian travel companies. Partial least square (PLS) path modelling approach, a variance-based structural equation modelling (VB-SEM), was used to assess the overall goodness-of-fit tests, measurement and structural model. The results highlight different aspects related to the effectiveness and attractiveness of travel companies’ websites. Its unique finding highlights the importance of personal value as a user characteristic factor that can strongly affect online purchase intention. In addition, by combining user characteristics and website characteristic and examining them in a single model, this study provides a clear multidimensional picture of causal relationship between latent constructs in an online travel purchase context. Theoretical and practical implications of study results are discussed and suggestions for future research are provided.
The significance of FM associations for women with FM

Abstract Purpose: Living with fibromyalgia (FM) means living with a long-term pain syndrome that is invisible to others. Support and understanding from others seem to be important to managing the affected daily life. The aim of this study was to describe the significance of FM associations for women with FM. Methods: Data collection was carried out through focus group discussions with seventeen women with FM. Data were analyzed through thematic content analysis. Results: The findings show that women experienced associations for people with FM as important as they gave access to contacts with others with similar experiences. Their need of togetherness was fulfilled at the association and they described being strengthened by the support received. Because of the lack of information and knowledge about FM, the association was described as an important venue for getting and mediating information about the illness. Conclusions: At the association the women seem to be empowered, which increases their ability to manage their daily lives despite the limitations imposed by FM. Healthcare personnel could not satisfy the women’s needs and to manage to support women with FM. There is a need for communication based on a shared understanding between the women and healthcare personnel. Implications for Rehabilitation This study highlighted the need for communication based on a shared understanding between people with chronic illness and healthcare personnel to support and strengthen women with FM in their daily lives. The FM associations meet the needs for togetherness, confirmation, and information that the women with FM in this study described and healthcare personnel could not satisfy. Healthcare personnel can learn from FM associations how to empower women with FM in their everyday lives.
Molecular marker systems in insects: current trends and future avenues

Insects comprise the largest species composition in the entire animal kingdom and possess a vast undiscovered genetic diversity and gene pool that can be better explored using molecular marker techniques. Current trends of application of DNA marker techniques in diverse domains of insect ecological studies show that mitochondrial DNA (mtDNA), microsatellites, random amplified polymorphic DNA (RAPD), expressed sequence tags (EST) and amplified fragment length polymorphism (AFLP) markers have contributed significantly for progresses towards understanding genetic basis of insect diversity and for mapping medically and agriculturally important genes and quantitative trait loci in insect pests. Apart from these popular marker systems, other novel approaches including transposon display, sequence‐specific amplification polymorphism (S‐SAP), repeat‐associated polymerase chain reaction (PCR) markers have been identified as alternate marker systems in insect studies. Besides, whole genome microarray and single nucleotide polymorphism (SNP) assays are becoming more popular to screen genome‐wide polymorphisms in fast and cost effective manner. However, use of such methodologies has not gained widespread popularity in entomological studies. The current study highlights the recent trends of applications of molecular markers in insect studies and explores the technological advancements in molecular marker tools and modern high throughput genotyping methodologies that may be applied in entomological researches for better understanding of insect ecology at molecular level.
Burden sharing and the future of NATO: wandering between two worlds

ABSTRACT The US role in the North Atlantic Treaty Organization (NATO) Alliance is a 65-year history of retrenchment and renewal. When Washington has sought a retrenchment from the world, it traditionally increased burden sharing pressure on Europe to do more. During times of increased global ambition, the USA reaffirmed its traditional leadership role in the Alliance and its commitment to NATO effectiveness and relevance. This cycle of NATO retrenchment and renewal, however, is halting. Though the USA will continue to go through periods of relative increases and decreases in security policy ambition, signs point to a permanent defense and security retrenchment in Europe. Germany is the ally singularly capable of filling the resulting security gap. If NATO is to avoid the drift toward irrelevance many critics have predicted, Germany will need to cast off old inhibitions toward security and defense leadership. These trends and their implications for NATO's future are explored through historical case studies and the shifting contemporary security environment.
Evaluation of potential prognostic value of Bmi-1 gene product and selected markers of proliferation (Ki-67) and apoptosis (p53) in the neuroblastoma group of tumors.

INTRODUCTION Cancer in children is a very important issue in pediatrics. The least satisfactory treatment outcome occurs among patients with clinically advanced neuroblastomas. Despite much research, the biology of this tumor still remains unclear, and new prognostic factors are sought. The Bmi-1 gene product is a currently highly investigated protein which belongs to the Polycomb group (PcG) and has been identified as a regulator of primary neural crest cells. It is believed that Bmi‑1 and N-myc act together and are both involved in the pathogenesis of neuroblastoma. The aim of the study was to assess the potential prognostic value of Bmi-1 protein and its relations with mechanisms of proliferation and apoptosis in the neuroblastoma group of tumors. MATERIAL/METHODS 29 formalin-fixed and paraffin-embedded neuroblastoma tissue sections were examined using mouse monoclonal antibodies anti-Bmi-1, anti-p53 and anti-Ki-67 according to the manufacturer's instructions. RESULTS There were found statistically significant correlations between Bmi-1 expression and tumor histology and age of patients. CONCLUSIONS Bmi-1 seems to be a promising marker in the neuroblastoma group of tumors whose expression correlates with widely accepted prognostic parameters. The pattern of BMI-1 expression may indicate that the examined protein is also involved in maturation processes in tumor tissue.
A Polymorphism in the Serotonin Transporter Gene Moderates Cardiovascular Reactivity to Psychosocial Stress

Objective: To examine whether a polymorphism (5-HTTLPR: serotonin transporter linked polymorphic region) in the promoter of the serotonin transporter gene (SLC6A4) moderates cardiovascular reactivity to social threat. Methods: Psychologically healthy young adults delivered a speech and performed mental arithmetic in one of three conditions: a) an evaluative audience condition that gave disapproving and negative nonverbal social signals (n = 59); b) an evaluative audience condition that provided supportive social signals (n = 60); or c) a no audience condition (n = 65). Heart rate (HR) and systolic and diastolic blood pressures (DBP) were measured before, during, and after the stress tasks to assess cardiovascular reactivity and recovery. Results: In the negative audience condition, there was a significant association between the 5-HTTLPR and systolic blood pressure, DBP, and HR reactivity. Individuals with the short/short genotype showed the greatest reactivity. The DBP and HR reactivity of short/short individuals in the negative audience condition was also greater than that of individuals with the short/short genotype in the no audience condition. These associations of the 5-HTLPR with HR reactivity were moderated by gender, being limited to females. With respect to cardiovascular recovery, short/short individuals in the negative audience condition exhibited impaired DBP recovery relative to other genotypes in the same condition, as well as short/short individuals in the no audience condition. Conclusions: The 5-HTTLPR moderates cardiovascular reactivity to stress in a threatening evaluative social context, which suggests that the serotonin system may be involved in the processes by which stressful, conflict-ridden social environments affect risk for cardiovascular-related health outcomes.5-HTTLPR = serotonin transporter gene linked polymorphic region; CVR = cardiovascular reactivity; SBP = systolic blood pressure; DBP = diastolic blood pressure; HR = heart rate.
Order Integration and the Dynamic Behavior of Security Prices

Strengthening competition in the equity markets has long been a major public policy objective. This paper turns to another important determinant of market quality, one that has received relatively little attention in the public policy debates: order integration — the way in which orders are matched together and turned into trades. A conceptual framework is set forth, and simulation analysis undertaken in a laboratory-type setting. We find that imperfect order integration can generate the accentuated intra-day volatility, kurtosis, and returns autocorrelation that characterize the dynamic behavior of prices. The analysis has important implications for market structure and regulatory policy.
Photoionization and photoelectric loading of barium ion traps

Simple and effective techniques for loading barium ions into linear Paul traps are demonstrated. Two-step photoionization of neutral barium is achieved using a weak intercombination line (6s{sup 2} {sup 1}S{sub 0}{r_reversible}6s6p {sup 3}P{sub 1}, {lambda}=791 nm) followed by excitation above the ionization threshold using a nitrogen gas laser ({lambda}=337 nm). Isotopic selectivity is achieved by using a near Doppler-free geometry for excitation of the triplet 6s6p {sup 3}P{sub 1} state. Additionally, we report a particularly simple and efficient trap loading technique that employs an inexpensive uv epoxy curing lamp to generate photoelectrons.
Further insights into the role of NIN-LIKE PROTEIN 7 (NLP7) in root cap cell release

ABSTRACT The root cap protects the root from environmental stress and senses gravity. Cells of the last layer of the root cap are shed in a developmentally programmed process. We previously showed that the transcription factor NIN-LIKE PROTEIN7 (NLP7) regulates root cap cell release likely through regulation of CELLULASE5 (CEL5). Here we provide a supplement to that work. We hypothesized that the nlp7 mutant has defects in additional root cap functions. We find that neither gravity sensing nor expression of a root cap cell identity marker is altered in nlp7 but that expression of another cellulase, CEL3, is upregulated. We conclude that NLP7 control of root cap cell release is largely independent of gravity sensing and root cap cell identity.
Asymptomatic internal carotid artery stenosis defined by ultrasound and the risk of subsequent stroke in the elderly. The Cardiovascular Health Study.

BACKGROUND AND PURPOSE We sought in this study to relate carotid ultrasound findings in asymptomatic older adults to the 5-year risk of various cerebrovascular outcomes used in the Asymptomatic Carotid Atherosclerosis Study (ACAS). METHODS The Cardiovascular Health Study (CHS) is a longitudinal study of people 65 years and older. Analyses of internal carotid artery stenosis defined by multiple different cutoffs of peak systolic velocity, rather than one particular cutoff, were performed in the 5441 participants who underwent carotid ultrasound and lacked a history of transient ischemic attack or stroke. The 5-year risks of 7 cerebrovascular disease outcomes used in ACAS were estimated for each cutoff. RESULTS Associations with the 5-year risk of outcomes were substantially elevated only at cutoffs with high peak systolic velocities. In this population, the number of people with such high velocities was small. For example, with a cutoff of approximately 2.5 m/s, suggesting a stenosis of >70%, the 5-year risk of an ipsilateral fatal or nonfatal stroke was 5%, and only 0.5% of the group had velocities at least this high. CONCLUSIONS In a group of older adults likely to participate in a screening program, as evidenced by willingness to participate in CHS, high peak systolic velocities consistent with high-grade carotid stenosis were uncommon and risk of subsequent cerebrovascular disease outcomes was relatively low. These findings do not suggest that similar populations of older adults would benefit from a program using ultrasound to screen for asymptomatic carotid stenosis.