Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of a Novel 135-kDa Grb2-binding Protein in Osteoclasts*

The tyrosine kinase receptor for macrophage colony-stimulating factor and the non-receptor tyrosine kinase c-Src play critical roles in osteoclast differentiation and function. Since the ubiquitously expressed adaptor protein Grb2 plays an important role in several tyrosine kinase signal transduction pathways, we used a filter binding assay to identify osteoclast proteins that bind to Grb2. In osteoclasts, there were three major Grb2-binding proteins, two of which, mSos and c-Cbl (p120), have been previously identified as Grb2-binding proteins in many cell types. The third protein, p135, had a restricted pattern of expression and was present at high levels in authentic osteoclasts and osteoclast-like cells formed in an in vitro co-culture system. In addition to binding Grb2 in the filter binding assay, p135 was isolated in complexes with endogenous Grb2 from osteoclast cell extracts. The association of p135 and Grb2 was dependent on an intact Src homology 3 domain and furthermore, was shown to preferentially interact with the N-terminal Src homology 3 domain of Grb2, which is similar to the interaction of mSos and Grb2 in other cell types. p135 was not recognized by antibodies against several known Grb2-binding proteins and thus may be a novel Grb2-binding protein.
ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI INDEKS PEMBANGUNAN MANUSIA DI INDONESIA PERIODE 2008-2012

Human Development Index (HDI) is an index established by UNDP in 1996 to measure the well-being of society. Indonesian HDI increased during the period of 2008-2012; however, there is high variation among 33 provinces. Its position in the world is still below the average (121 of 187 countries) and ranks 5th among ASEAN countries (UNDP, 2013). Based on this phenomenon this study tries to analyze the determinants of HDI which include GDP, dependency ratio, household consumption for food, government budget for education, and the budget for health. The results indicate that GDP and government budget for health have positive effect on HDI, whereas dependency ratio and household consumption for food have negative effect. However, the budget for education has no effect on HDI. HDI is an important indicator of development; therefore, the government and the society should take efforts to improve the HDI. Economic growth, income distribution, population control, poverty alleviation, as well as improvement in health services and education are needed in order to improve the HDI.
Crossing Boundaries in Literacy Research

The concept of boundaries, as borders that separate two entities, can be problematic in that those who “belong” within a boundary in a given social world or entity are separated from those who do not, creating binaries. The field of literacy research has had a long history of binarism in which instructional methods, epistemological positions, and paradigms have been pitted against one another. An ontological discussion of the nature of boundaries, combined with a content analysis of journal citations, revealed that current literacy research is characterized more as multidisciplinary rather than interdisciplinary, suggesting that literacy research may be characterized by disciplinary binarism. A situational analysis of the social worlds and arenas present in an interdisciplinary research project illustrates the complexity of interdisciplinary partnerships and the manner in which boundary objects can be created to negotiate boundaries and avoid intellectual, disciplinary, epistemological, and methodological nativism.
CLASSIFICATION OF SATELLITE RESONANCES IN THE SOLAR SYSTEM

Several pairs of solar system satellites occupy mean motion resonances (MMRs). We divide these into two groups according to their proximity to exact resonance. Proximity is measured by the existence of a separatrix in phase space. MMRs between Io–Europa, Europa–Ganymede, and Enceladus–Dione are too distant from exact resonance for a separatrix to appear. A separatrix is present only in the phase spaces of the Mimas–Tethys and Titan–Hyperion MMRs, and their resonant arguments are the only ones to exhibit substantial librations. Could there be a causal connection between the libration amplitude and the presence of a separatrix? Our suspicions were aroused by Goldreich & Schlichting, who demonstrate that sufficiently deep in a MMR, eccentricity damping could destabilize librations. However, our investigation reveals that libration amplitudes in both the Mimas–Tethys and Titan–Hyperion MMRs are fossils. Although the Mimas–Tethys MMR is overstable, its libration amplitude grows on the tidal damping timescale of Mimas’s inclination, which is considerably longer than a Hubble time. On the other hand, the Titan–Hyperion MMR is stable, but tidal damping of Hyperion’s eccentricity is too weak to have affected the amplitude of its libration.
Multi-mJ single-filament supercontinuum generation from IR OPCPA

Recently, we have demonstrated multimillijoule optically synchronized and passively carrier-envelope-phase (CEP) stable optical parametric chirped-pulse amplification (OPCPA) at 1.5 µm [1]. Our four-stage infrared OPCPA system is based on a DPSS Yb-MOPA-driven (Pharos, Light Conversion Ltd.), CEP-stable front-end [2] and two successive power-amplification stages pumped by a 100-mJ 60-ps 20-Hz 1.064-µm Nd:YAG amplifier (Ekspla Ltd.), which is seeded from the Yb master oscillator [1]. In the amplification stages 2–4, we employ (nearly) collinear type-II phase matching in KTP/KTA reaching the pulse energy of ∼12.5 mJ at a 20-Hz repetition rate before a grating compressor.
icaA Is Not a Useful Diagnostic Marker for Prosthetic Joint Infection

ABSTRACT A collection of 99 staphylococcal isolates associated with prosthetic joint infection and 23 coagulase-negative staphylococci isolated from noninfected arthroplasty-associated specimens were screened in order to determine whether the presence of icaA could be used to distinguish between pathogens and nonpathogens. All Staphylococcus aureus prosthetic joint infection isolates (n = 55) were icaA positive. A total of 46% (20 out of 44) of coagulase-negative staphylococcal prosthetic joint infection isolates were icaA positive, and 30% (7 out of 23) of arthroplasty-associated non-prosthetic joint infection-associated coagulase-negative staphylococcal isolates were icaA positive (P = 0.23). Certain coagulase-negative Staphylococcus species appeared more likely to be isolated as either arthroplasty-associated non-prosthetic joint infection-associated isolates (e.g., Staphylococcus warneri and Staphylococcus hominis) or pathogens (e.g., Staphylococcus lugdunensis). The presence of icaA in a coagulase-negative staphylococcal isolate associated with an arthroplasty is not a useful diagnostic indicator of pathogenicity.
Clinical Trials and Lipid Guidelines for Type II Diabetes

The management of dyslipidemia in adults with diabetes is receiving more attention. However, there is a paucity of large, prospective, randomized outcome trials designed for diabetic patients. Diabetic dyslipidemia is characterized by an increase in triglyceride levels, low high‐density lipoprotein (HDL) cholesterol concentrations, and small, dense low‐density lipoprotein (LDL) particles. The treatment goals include an LDL cholesterol less than 100 mg/dL, triglyceride level less than 150 mg/dL, and an HDL greater than 40 mg/dL for men and more than 50 mg/dL for women. In the Diabetic Atherosclerosis Intervention Study, fenofibrate resulted in a 42% less increase in the percent stenosis, as assessed by quantitative coronary arteriography. The Heart Protection Study documented the unambiguous benefit of simvastatin in reducing all‐cause mortality among 5963 diabetic patients. The Lescol Intervention Prevention Study observed a reduction in major adverse cardiac events in diabetics undergoing percutaneous intervention who received fluvastatin. The Veterans Affairs HDL Cholesterol Intervention Trial reported a reduction in major coronary events among 627 diabetic patients with low HDL cholesterol who sustained a myocardial infarction. The Fenofibrate Intervention and Event Lowering in Diabetics (FIELD) Trial (n = 9795), the Action to Control Cardiovascular Risk in Diabetes (ACCORD, n = 10,000), the Atorvastatin Study for Prevention of Coronary Heart Disease Endpoints in Non Insulin Dependent Diabetes Mellitus (ASPEN, n = 2421), and the Collaborative Atorvastatin Diabetes Study (CARDS, n = 2140) will provide the prospective outcome data that are needed for the management of patients. Combination drug therapy will be necessary to achieve treatment goals. Careful monitoring will be required to avoid myositis and hepatotoxicity.
Diel regulation of photosynthetic activity in the oceanic unicellular diazotrophic cyanobacterium Crocosphaera watsonii WH8501

The oceanic unicellular diazotrophic cyanobacterium Crocosphaera watsonii WH8501 exhibits large diel changes in abundance of both Photosystem II (PSII) and Photosystem I (PSI). To understand the mechanisms underlying these dynamics, we assessed photosynthetic parameters, photosystem abundance and composition, and chlorophyll-protein biosynthesis over a diel cycle. Our data show that the decline in PSII activity and abundance observed during the dark period was related to a light-induced modification of PSII, which, in combination with the suppressed synthesis of membrane proteins, resulted in monomerization and gradual disassembly of a large portion of PSII core complexes. In the remaining population of assembled PSII monomeric complexes, we detected the non-functional version of the D1 protein, rD1, which was absent in PSII during the light phase. During the dark period, we also observed a significant decoupling of phycobilisomes from PSII and a decline in the chlorophyll a quota, which matched the complete loss of functional PSIIs and a substantial decrease in PSI abundance. However, the remaining PSI complexes maintained their photochemical activity. Thus, during the nocturnal period of nitrogen fixation C. watsonii operates a suite of regulatory mechanisms for efficient utilization/recycling of cellular resources and protection of the nitrogenase enzyme.
Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice.

Basic fibroblast growth factor (bFGF) has been shown to stimulate wound healing. However, consistent delivery of bFGF has been problematic. We studied the stability of bFGF incorporated into a chitosan film as a delivery vehicle for providing sustained release of bFGF. The therapeutic effect of this system on wound healing in genetically diabetic mice was determined as a model for treating clinically impaired wound healing. A chitosan film was prepared by freeze-drying hydroxypropylchitosan (a water-soluble derivative of chitosan) acetate buffer solution. Growth factor was incorporated into films before drying by mixing bFGF solution with the hydroxypropylchitosan solution. bFGF activity remained stable for 21 days at 5 degrees C, and 86.2% of activity remained with storage at 25 degrees C. Full-thickness wounds were created on the backs of diabetic mice, and chitosan film or bFGF-chitosan film was applied to the wound. The wound was smaller in after 5 days in both groups, but the wound was smaller on day 20 only in the bFGF-chitosan group. Proliferation of fibroblasts and an increase in the number of capillaries were observed in both groups, but granulation tissue was more abundant in the bFGF-chitosan group. These results suggest that chitosan itself facilitates wound repair and that bFGF incorporated into chitosan film is a stabile delivery vehicle for accelerating wound healing.
Cell Magnetic Targeting System for Repair of Severe Chronic Osteochondral Defect in a Rabbit Model

The aim of this study was to investigate a cell delivery system for repair of severe chronic osteochondral defects using magnetically labeled mesenchymal stem cells (m-MSCs), with the aid of an external magnetic device, through the accumulation of a small number of m-MSCs into a desired area and to detect the suitable number of autologous m-MSCs needed for repair of the defect. Twenty-six male Japanese white rabbits aged 6 months were used. An osteochondral defect was created bilaterally at the weight-bearing surface of the medial femoral condyle of the rabbits' knees (3 mm diameter; 4 mm depth). At 4 weeks after creation of the defect, autogenic transplantation of the m-MSCs into the defect area was performed, followed by 10-min exposure to an external magnetic device, where animals were divided into four groups: high (1 × 106 m-MSCs), medium (2 × 105 m-MSCs), low (4 × 104 m-MSCs), and control (PBS injection). At 4 and 12 weeks posttransplantation of m-MSCs, repaired tissue was assessed histologically using the Fortier score with toluidine blue staining. Transplantation of a low number of m-MSCs was not enough to improve osteogenesis and chondrogenesis, but the medium and high groups improved repair of the chronic defect with chondrogenic tissues and showed histologically significantly better results than the control and low groups. The use of a magnetic targeting system for delivering m-MSCs has the potential to overcome the clinical hurdles for repair of the severe chronic osteochondral defect. Furthermore, this system is predicted to produce good clinical outcomes for humans, not only to repair osteochondral defects but also to repair a variety of damaged tissues.
BetweenIT: An Interactive Tool for Tight Inbetweening

The generation of inbetween frames that interpolate a given set of key frames is a major component in the production of a 2D feature animation. Our objective is to considerably reduce the cost of the inbetweening phase by offering an intuitive and effective interactive environment that automates inbetweening when possible while allowing the artist to guide, complement, or override the results. Tight inbetweens, which interpolate similar key frames, are particularly time‐consuming and tedious to draw. Therefore, we focus on automating these high‐precision and expensive portions of the process. We have designed a set of user‐guided semi‐automatic techniques that fit well with current practice and minimize the number of required artist‐gestures. We present a novel technique for stroke interpolation from only two keys which combines a stroke motion constructed from logarithmic spiral vertex trajectories with a stroke deformation based on curvature averaging and twisting warps. We discuss our system in the context of a feature animation production environment and evaluate our approach with real production data.
When will we stop studying innovations in organizing, and start creating them?

Abstract Rather than being passive observers and analysts of innovations in organizing, I argue that we can advance organizational science by prototyping and piloting new forms of organizing. While this may be a radical departure from our conventional ways of studying organizations, I believe that the return is worth the risk, particulary in a normatively oriented field like organizaion design. Equally important, recent conceptual and methodological advances actually make this possible.
Psychoaffective Immaturity in Psychiatric Disorders

This study investigated the prevalence of psychoaffective immaturity and tested the hypothesis that it associated with bad prognosis. For 135 psychiatric patients meeting criteria for personality, neurotic, affective, substance use, or psychotic disorders emotional immaturity was rated using the 1985 diagnostic criteria of Doutheau, Dubertret, Moutin, and Barrois. 58 subjects (42.96%, 95% Confidence Interval: 34.61–51.31) were classified as immature. Scores of the Nonimmature and Immature groups were compared for the Beck Depression Inventory and the Professional and Social Functioning Assessment Scale. Scores were, respectively, significantly higher and lower in those patients classified as Immature than those who were classified Nonimmature. When depression was controlled by a covariance analysis, the mean difference on the Professional and Social Functioning Assessment Scale remained significant. It appears that psychoaffective immaturity is a factor associated with severity of psychiatric disorders.
Implementation and evaluation of cooperative video streaming for mobile devices

A promising approach for power reduction in mobile devices is peer-to-peer cooperation. In this paper, we present a testbed system implementation to evaluate the performance of an infrastructure controlled cooperative video streaming architecture. A number of mobile devices in close proximity are connected to the Internet through a wireless access point and are all requesting the same video stream from a dedicated server. Instead of sending the stream independently to each device, the server distributes the video packets among the devices, over a long-range wireless link using WLAN technology; the devices then exchange the received packets among each other over short-range wireless links using Bluetooth technology. The implemented testbed is used to experimentally demonstrate the power reduction gains in mobile devices and to derive an analytical model that relates power consumption to various design and system parameters.
GP-m: Mobile middleware infrastructure for Ambient Assisted Living

The problem of providing assistive services to elderly in smart cities is becoming important due to the aging of population in the developed countries. The possibility of using personal devices like smartphones to be assisted also outside the house is a key factor to guarantee the independency of elderly users still remaining connected to his caregivers network. We identified in the development of a suitable mobile middleware one of the main solution to the barrier in the deployment of distributed AAL services. In this scenario, we show the effectiveness of the mobile middleware solution proposed, called GiraffPlus-mobile (GP-m), in terms of integration with the existing pervasive environment, performances and energy saving.
The liberal commercial peace, regional considerations: International relations of Southeast Asia, Latin America, and the European Union countries

Most of the large-N empirical studies on the liberal commercial peace theory demonstrate that, in general, trade has a robust pacifying effect in reducing the probability of dyadical militarized interstate disputes. However, why trade’s pacifying effect varies across time and space remains a puzzle that previous research has not yet solved. The liberal commercial peace literature suggests the following questions are in need of answers: Why does trade promote peace mainly in inter-region dyads but not in intra-region dyads? What are the preconditions that make trade’s pacifying effect work or not work? In this research, I argue that there are two critical preconditions for trade’s pacifying effect to work: first, the benefits of trade must be substantively important, and second, important social actors who have stakes on trade must be able to influence the leaders when they are making foreign policy decisions. These two preconditions can be measured by countries’ degree of democracy and degree of development. Therefore, regionally, the pacifying effect of trade is stronger in regions where the countries are more democratic and more developed, and it gets weaker in regions where the countries are less democratic and less developed; temporally, because countries’ degree of democracy and degree of development vary from time to time, the pacifying effect of trade changes over time accordingly. I then extend this overarching framework to investigate the role of trade in the regional peace of Southeast Asia, Latin America, and the European Union countries, arguing that the variations of the regional peace can be predicted by looking at how trade affects the most important political issues in each of the
Quantification of the glycogen 13C‐1 NMR signal during glycogen synthesis in perfused rat liver

We studied glycogen synthesis from glucose in perfused livers of fed (n = 4) and 24 h starved (n = 7) rats. Glycogenolysis was inhibited by BAY R3401 (150 µM) and proglycosyn (100 µM). After 60 min, we replaced 99% 13C‐1 glucose by natural abundance glucose. This pulse‐chase design allowed us to recognize residual ongoing futile glycogen turnover from the release of initially deposited 13C‐label, into the 13C‐free chase medium. Net residual turnover was less than 2 ± 0.7% and 0.6 ± 0.2% of 1‐13C glycogen deposition rates of 0.31 ± 0.04 and 0.99 ± 0.04 µmol glucose g−1 min−1, in starved and fed livers, respectively. The 1‐13C glycogen signal was monitored throughout the experiment with proton‐decoupled 13C NMR spectroscopy and analyzed in the time domain using AMARES. We noticed progressive line‐broadening in any single experiment in the chase phase. One or a sum of two to three overlapping Lorentzians, with different exponential damping factors, were fitted to the signal. When the S/N was better than 40, the fit always delivered a small and a broad component. In the chase phase, the fit with a single Lorentzian resulted in a decline of glycogen signal by about 15 ± 4 and 12 ± 2% in starved and fed rats, respectively. This apparent decline in 1‐13C glycogen signal could not be accounted for by the appearance of equivalent amounts of 13C‐labeled metabolites in the perfusate. The fit with a sum of two Lorentzians resulted in a decline of glycogen signal intensity of 7 ± 5 and 5 ± 3% in starved and fed rats, respectively, which reduced the apparent turnover to 8 ± 9% and 6 ± 4%, respectively. Quantification of the growing 13C‐1 glycogen signal requires a model function that accommodates changes in line shape throughout the period under study. Copyright © 2003 John Wiley & Sons, Ltd.
The physical demands upon (Dutch) fire-fighters in relation to the maximum acceptable energetic workload

The aim of this study was to assess the tasks and activities that make physical demands on Dutch fire-fighters and to compare them with a guideline related to the development of excessive fatigue. The occupational physical demands on Dutch fire-fighters were assessed by conducting a task analysis during 85 24-h shifts. While workplace observations on the duration and frequency of physical tasks and activities were recorded, the heart rate was measured. This was then used to calculate the heart rate reserve percentage (%HRR) for predefined working periods, tasks and activities during 24-h shifts. The findings indicate that actual fire-fighting during 24-h shifts is characterised by a low frequency of incidents, a short ‘turn-out’ time, short tasks, and activities with a moderate to occasionally high energetic workload. Two tasks which sometimes occur in actual fire-fighting exceeded the guideline on energetic workload. The conclusion was that, though the number of incidents and the occupational demands are low during 24-h shifts, the peak loads for these two tasks are energetically high and could lead to excessive fatigue. Consequently, attention may need to be paid to health surveillance for persons exposed to such energetic peak loads, the development of physical and medical selection procedures, training, and workplace adjustments.