Miracle-dz/UniFAR-FTUs huggingface dataset:
Liver-Infiltrating Lymphocytes in Chronic Human Hepatitis C Virus Infection Display an Exhausted Phenotype with High Levels of PD-1 and Low Levels of CD127 Expression

ABSTRACT The majority of people infected with hepatitis C virus (HCV) fail to generate or maintain a T-cell response effective for viral clearance. Evidence from murine chronic viral infections shows that expression of the coinhibitory molecule PD-1 predicts CD8+ antiviral T-cell exhaustion and may contribute to inadequate pathogen control. To investigate whether human CD8+ T cells express PD-1 and demonstrate a dysfunctional phenotype during chronic HCV infection, peripheral and intrahepatic HCV-specific CD8+ T cells were examined. We found that in chronic HCV infection, peripheral HCV-specific T cells express high levels of PD-1 and that blockade of the PD-1/PD-L1 interaction led to an enhanced proliferative capacity. Importantly, intrahepatic HCV-specific T cells, in contrast to those in the periphery, express not only high levels of PD-1 but also decreased interleukin-7 receptor alpha (CD127), an exhausted phenotype that was HCV antigen specific and compartmentalized to the liver, the site of viral replication.
Mobile Device Passive Localization Based on IEEE 802.11 Probe Request Frames

This paper presents a novel passive mobile device localization mode based on IEEE 802.11 Probe Request frames. In this approach, the listener can discover mobile devices by receiving the Probe Request frames and localize them on his walking path. The unique location of the mobile device is estimated on a geometric diagram and right-angled walking path. In model equations, site-related parameter, that is, path loss exponent, is eliminated to make the approach site-independent. To implement unique localization, the right-angled walking path is designed and the optimal location is estimated from the optional points. The performance of our method has been evaluated inside the room, outside the room, and in outdoor scenarios. Three kinds of walking paths, for example, horizontal, vertical, and slanted, are also tested.
Can Neuroscience Advance Our Understanding of Core Questions in Communication Studies? An Overview of Communication Neuroscience

Can neuroimaging methods oHer any beneJt to communication scholars? Although communication scholars draw on multiple, interdisciplinary methods, the Jeld has not traditionally leveraged neuroimaging techniques (Cappella, 1996). By contrast, other social science disciplines have beneJKed greatly from the use of neuroscience methodologies to test core theoretical questions (Adolphs, 2003; Cabeza & Nyberg, 2000a; Cacioppo, 2002; Cacioppo, Berntson, Sheridan, & McClintock, 2000; Lieberman, 2010; Loewenstein, Rick, & Cohen, 2008; Ochsner & Lieberman, 2001; Poldrack, 2008; Sanfey, Loewenstein, & Mcclure, 2006; Yarkoni, Poldrack, Van Essen, & Wager, 2010). Ie current chapter outlines a vision for how communication studies might leverage neuroimaging technologies moving forward. We begin by deJning communication neuroscience as a subdiscipline and giving a brief overview of the most commonly employed neuroimaging methods. We follow this introduction with a discussion of the types of questions that neuroimaging is most equipped to answer and suggest areas for further exploration.
Probabilistic Latent Semantic Analysis

Probabilistic Latent Semantic Analysis (pLSA) is a technique from the category of topic models. Its main goal is to model cooccurrence information under a probabilistic framework in order to discover the underlying semantic structure of the data. It was developed in 1999 by Th. Hofmann [7] and it was initially used for text-based applications (such as indexing, retrieval, clustering); however its use shortly spread in other fields: such as computer vision [14, 16, 10] or audio processing [5]. PLSA can be regarded in two seemingly different ways:
Global Optimization for Neural Network Training

We propose a novel global minimization method, called NOVEL (Nonlinear Optimization via External Lead), and demonstrate its superior performance on neural network learning problems. The goal is improved learning of application problems that achieves either smaller networks or less error prone networks of the same size. This training method combines global and local searches to find a good local minimum. In benchmark comparisons against the best global optimization algorithms, it demonstrates superior performance improvement.
Medição da autonomia em atividades da vida diária

Introdução: As atividades da vida diária constituem o nível mais básico de autonomia, que permitem a participação no dia-a-dia em termos de sobrevivência e bem-estar básicos. Neste trabalho, focaremos a necessidade de avaliar as atividades da vida diária em cuidados continuados e paliativos. Objetivo: Descrever e comparar instrumentos de medição de atividades da vida diária. Material e métodos: Pesquisa bibliográfica de instrumentos de medição de atividades de vida diária, em inglês e português, entre 1980 e 2017, nas bases de dados científicas reconhecidas para este efeito. Resultados: Encontraram-se seis instrumentos de medição e, para cada um, identificou-se o número de perguntas e dimensões avaliadas, as propriedades psicométricas e a existência de versão portuguesa validada. Discussão: Todos os instrumentos são preenchidos pelo profissional, por observação direta, e avaliam os autocuidados. Vestir/despir, alimentação e mobilidade são atividades comuns a todos, havendo outras específicas de apenas uma escala. Conclusão: As dimensões vestir/despir, alimentação e mobilidade são as mais avaliadas. Apenas o índice de Barthel está validado para a população portuguesa. Quanto a critérios de qualidade, uma escala apresenta fiabilidade para o acidente vascular cerebral. A maioria das escalas apresenta boa correlação com outras escalas de avaliação da autonomia.
The time course of access to semantic information in high-performing older adults: Behavioral evidence for the hemispheric asymmetry reduction in OLDer individuals

ABSTRACT The possibility that the HAROLD phenomenon (i.e., Hemispheric Asymmetry Reduction in OLDer adults) is manifested in the course of access to semantic information, in particular the meaning of emotional words, was investigated using the visual half-field priming paradigm. The time course of priming was tracked in the cerebral hemispheres across three SOAs: 150, 300, and 750 ms. The results showed older and young adults had the same level of accuracy. While priming occurred unilaterally in young participants, the pattern of priming in older participants appeared to be bilateral whenever it was present, that is, at the 300- and 750-ms SOAs. The delay in the appearance of priming in older adults may be due to an increase in sensory thresholds that causes older adults to need more time to encode stimuli and fully activate their semantic network. It is concluded that the bilateral pattern of priming in the presence of an equivalent level of performance in older adults provides behavioral evidence supporting the compensatory role of the HAROLD phenomenon for this particular task.
P-glycoprotein Potentiates CYP3A4-mediated Drug Disappearance during Caco-2 Intestinal Secretory Detoxification

Human intestinal Caco-2 cell monolayers grown in the presence of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) were used to test the hypothesis that drugs which interact with the apical efflux pump P-glycoprotein (Pgp) may enhance CYP3A4-mediated disappearance of substrates. 6β-Hydroxytestosterone production, a marker of CYP3A4 activity, was approximately 3- and 7-fold greater in 1,25(OH)2D3-treated cells compared to untreated cells when incubated with 50 and 500 μM testosterone, respectively, and was unaffected by the addition of digoxin to reduce Pgp activity. In the presence of digoxin, secretory transport of vinblastine and erythromycin, substrates for both Pgp and cytochrome P450 3A4 (CYP3A4), was significantly reduced, whereas absorptive transport was unaffected. In contrast, no directional transport of testosterone, a substrate for CYP3A4 only, was observed, either in the presence or absence of digoxin. Over 2 h, disappearance of erythromycin and vinblastine from the incubation medium was significantly greater from the basolateral than from the apical compartments. In the presence of digoxin, disappearance of both compounds from the basolateral, but not from the apical compartments, was significantly reduced. In contrast, disappearance of testosterone was unaffected by the addition of digoxin, demonstrating that the effect of digoxin on erythromycin and vinblastine disappearance was via inhibition of Pgp function, rather than on CYP3A4 activity. Thus, evidence is provided for Pgp/CYP3A4 co-substrates, Pgp potentiates CYP3A4-mediated drug disappearance during intestinal secretory detoxification.
Shadow Algorithms for Real-time Rendering

Shadows are crucial for enhancing realism and provide important visual cues. In recent years, many important contributions have been made both for hard shadows and soft shadows. Often spurred by the tremendous increase of computational power and capabilities of graphics hardware, much progress has been made concerning visual quality and speed, making high-quality real-time shadows a reachable goal. But with the growing wealth of available choices, it is particularly difficult to pick the right solution and assess shortcomings. Because currently there is no ultimate approach available, algorithms should be selected in accordance to the context in which shadows are produced. The possibilities range across a wide spectrum; from very approximate but really efficient to slower but accurate, adapted only to smaller or only to larger sources, addressing directional lights or positional lights, or involving GPUor CPU-heavy computations. This tutorial tries to serve as a guide to better understand limitations and failure cases, advantages and disadvantages, and suitability of the algorithms for different application scenarios. We will focus on real-time to interactive solutions but also discuss offline approaches if needed for a better understanding. In the future, we will provide you with more information via our webpage: http://www.mpi-inf.mpg. de/resources/ShadowCourse/.
Nonionic surfactant-based vesicular system for transdermal drug delivery

Abstract Objective: The objective of this study was to formulate and evaluate the Ibuprofen niosomal formulation as a transdermal drug delivery system. Materials and methods: Niosomes were prepared by a modified ethanol injection method, using Span 60, Tween 60 and Tween 65 as well as cholesterol with various cholesterol:surfactant molar ratios. The prepared vesicles were characterized for entrapment efficiency (EE), particle size, zeta potential and in vitro release study. Skin permeation studies were conducted using modified Franz diffusion cell, and excised rat skin was treated with niosomal, liposomal and conventional Carbopol 914 gel of Ibuprofen. Results and discussion: The results showed that the type of surfactant and molar ratio of cholesterol:surfactant altered the EE, size and in vitro drug release of niosomes. Higher EE was obtained with the niosomes prepared with cholesterol and Span 60 at molar ratio of 0.5:1. It has been observed that both niosomal and liposomal formulations enhanced the drug permeation and the percentage of accumulated dose in the skin compared to control conventional gel formulation. However, niosomes prepared by Span 60 and Tween 65 exhibited higher permeation and retention of Ibuprofen, respectively. Conclusion: Our results suggested that niosomal formulations could be used as a promising carrier for the Ibuprofen transdermal delivery system.
Tax and Benefit Reforms in a Model of Labour Market Transitions

This paper presents a method for taking advantage of labour market transitions to identify the effects of financial incentives on employment decisions. The framework used is very flexible and by imposing few theoretical assumptions it allows us to extend the modelled sample relative to structural models. The authors take advantage of this flexibility to include disabled persons in the model and to jointly analyse the behaviour of disabled and non-disabled persons. A great deal of attention is paid to the appropriate modelling of financial incentives in the labour market. In the case of disabled persons, taking account of financial incentives turns out to be an extremely complex process but one that in the end turns out to be well worth the effort. The model is used to compare reactions in the labour market to marginal changes in financial incentives and also to model one of the most important reforms of the UK Labour government – the introduction of the Working Families’ Tax Credit. The methodology relies on matching the transition and income data derived from cross-sectional and panel surveys, and could be used in other countries for which detailed, reliable income data are not collected in a panel format.
Study on the genetic diversity of native chickens in northwest Ethiopia using microsatellite markers.

In this study, indigenous chicken populations representing seven different areas of northwest Ethiopia were studied using microsatellite markers to determine genetic diversity and variation. Three local lines of South African chicken and two commercial chicken strains were included for comparison. The Ethiopian chicken population Gassay/Farta had the highest number of alleles per locus (10) for microsatellite marker MCW 158. MCW 154 was the most polymorphic marker across all populations with an average of seven different alleles. A high genetic diversity was observed in overall loci for all populations with heterozygosity (Ho) value of 0.77. The highest heterozygosity (0.93) across all markers was observed in the Mecha chicken population, while the lowest heterozygosity across all loci (0.66) was observed in the White Leghorn breed. The RIR commercial chicken breed showed higher genetic distance (lower genetic similarity) with the Ethiopian chicken populations than the South African fowls. This indicates that the Ethiopian indigenous chicken populations are still not highly diluted by the RIR chicken breed either through the extension program or through the regional poultry breeding and multiplication institutes. Based on the phylogenetic tree result, it is concluded that the clustering of the chicken populations in the present study are in accordance with the origin and marketing systems of these native chickens, which indicates that the microsatellite markers used in this study were suitable for the measurement of the genetic biodiversity and relationship of Ethiopian chicken populations. These results can therefore serve as an initial step to plan the characterization and conservation of native chickens in Amhara region, Ethiopia.
Impaired antiviral response in human hepatoma cells.

Hepatitis B, C, and D viruses can infect liver cells and in some individuals establish a chronic phase of infection. Presently, relatively little information is available on the antiviral mechanisms in liver cells. Because no good in vitro model infection systems for hepatitis viruses are available, we have used influenza A, Sendai, and vesicular stomatitis (VSV) viruses to characterize interferon (IFN) responses and IFN-induced antiviral mechanisms in human hepatoma cell lines. HepG2 or HuH7 cells did not show any detectable IFN-alpha/beta production in response to influenza A or Sendai virus infections. Treatment of cells with IFN-alpha resulted in upregulation of IFN-alpha-inducible Mx, 2',5'-oligoadenylate synthetase (OAS) and HLA class I gene expression but only with exceptionally high levels of IFN-alpha (>/=100 IU/ml). Accordingly, high pretreatment levels of IFN-alpha, 1000 IU/ml for influenza A and VSV and 100 IU/ml for Sendai virus, were required before any detectable antiviral activity against these viruses was seen. IFN-gamma had some antiviral effect against influenza A virus but appeared to be ineffective against VSV and Sendai virus. IFN-gamma upregulated HLA class I protein expression, whereas Mx or OAS expression levels were not increased. There was a modest upregulation of HLA class I expression during Sendai virus infection, whereas influenza A virus infection resulted, after an initial weak upregulation, in a clear decrease in HLA class I expression at late times of infection. The results suggest that hepatoma cells may have intrinsically poor ability to produce and respond to type I IFNs, which may contribute to their inability to efficiently resist viral infections.
Biodegradation of Direct Blue 15 by Free and Immobilized Trametes versicolor

To investigate biodegradability by Trametes versicolor, five structurally different direct azo‐dyes—Direct Black 38, Direct Blue 15 (DB 15), Direct Orange 26, Direct Green 6, and Direct Yellow 12—were studied. The DB 15 was determined as the best biodegradable dye by this white‐rot fungus. Laccase and manganese peroxidase activities were monitored with the biodegradation process; it was observed that laccase played an important role in the dye degradation, while manganese peroxidase activity could not be detected. Possible degradation products also were examined by gas chromatography‐mass spectrometry, but no metabolite was detected after the degradation and/or decolorization process. To enhance performance of the fungi during the degradation, Trametes versicolor cells were immobilized in alginate beads. Then, DB 15 decolorization by immobilized Trametes versicolor was studied in a small‐scale packed‐bed reactor. The color removal efficiency in repeated batches was found to be 98 and 93% for 50 mg/L DB 15.
Structural Implications on the Interaction of Scorpion α‐like Toxins with the Sodium Channel Receptor Site Inferred from Toxin Iodination and pH‐Dependent Binding

Abstract: The α‐like toxin from the venom of the scorpion Leiurus quinquestriatus hebraeus (Lqh III) binds with high affinity to receptor site 3 on insect sodium channels but does not bind to rat brain synaptosomes. The binding affinity of Lqh III to cockroach neuronal membranes was fivefold higher at pH 6.5 than at pH 7.5. This correlated with an increase in the electropositive charge on the toxin surface resulting from protonation of its four histidines. Radioiodination of Tyr14 of Lqh III abolished its binding to locust but not cockroach sodium channels, whereas the noniodinated toxin bound equally well to both neuronal preparations. Radioiodination of Tyr10 or Tyr21 of the structurally similar α‐toxin from L. quinquestriatus hebraeus (LqhαIT), as well as their substitution by phenylalanine, had only minor effects on binding to cockroach neuronal membranes. However, substitution of Tyr21, but not Tyr14, by leucine decreased the binding affinity of LqhαIT ∼87‐fold. Thus, Tyr14 is involved in the bioactivity of Lqh III to locust receptor site 3 and is not crucial for the binding of LqhαIT to this site. In turn, the aromatic ring of Tyr21 takes part in the bioactivity of LqhαIT to insects. These results highlight subtle architectural variations between locust and cockroach receptor site 3, in addition to previous results demonstrating the competence of Lqh III to differentiate between insect and mammalian sodium channel subtypes.
Emotional segregation: A content analysis of institutional racism in US films, 1980–2001

Abstract The purpose of this article is to empirically demonstrate a concept, known as “emotional segregation” through a systematic analysis of US films. Emotional segregation is defined as an institutionalized process, whereby European Americans are unable to see people of colour as emotional equals or as capable of sharing the same human emotions and experiences. This concept was inspired by Charles C. Stember's (1976) study of sexual racism and Patricia Hill Collins’ (2000) work on sexualized racism. In order to examine the existence of emotional segregation in the United States, a content analysis was conducted of forty US films chosen from a stratified random sample. Differences were found in media portrayals of inter- and intra-racial relationships involving African Americans. Future research on emotional segregation is suggested to assess how this phenomenon creates emotional barriers among ethnic groups.
Sodium Fluxes through Nonselective Cation Channels in the Plasma Membrane of Protoplasts from Arabidopsis Roots1

The aim of the present work was to characterize Na+currents through nonselective cation channels (NSCCs) in protoplasts derived from root cells of Arabidopsis. The procedure of the protoplast isolation was modified to increase the stability of Arabidopsis root protoplasts in low external Ca2+ by digesting tissue in elevated Ca2+. Experiments in whole-cell and outside-out modes were carried out. We found that Na+ currents in Arabidopsis root protoplasts were mediated by cation channels that were insensitive to externally applied tetraethylammonium+ and verapamil, had no time-dependent activation (permanently opened or completely activated within 1–2 ms), were voltage independent, and were weakly selective for monovalent cations. The selectivity sequence was as follows: K+ (1.49) > NH4 + (1.24) > Rb+ (1.15) ≈ Cs+ (1.10) ≈ Na+ (1.00) > Li+ (0.73) > tetraethylammonium+ (0.47). Arabidopsis root NSCCs were blocked by H+ (pK ≈ 6.0), Ca2+(K1/2 ≈ 0.1 mm), Ba2+, Zn2+, La3+, Gd3+, quinine, and the His modifier diethylpyrocarbonate. They were insensitive to most organic blockers (nifedipine, verapamil, flufenamate, and amiloride) and to the SH-group modifier p-chloromercuriphenyl sulfonic acid. Voltage-insensitive, Ca2+-sensitive single channels were also resolved. Properties of Arabidopsis root NSCCs are discussed and compared with characteristics of similar conductances studied previously in plants and animals. It is suggested that NSCCs present a distinct group of plant ion channels, mediating toxic Na+ influx to the cell and probably having other important roles in physiological processes of plants.
MIPS: analysis and annotation of proteins from whole genomes in 2005

The Munich Information Center for Protein Sequences (MIPS-GSF), Neuherberg, Germany, provides protein sequence-related information based on whole-genome analysis. The main focus of the work is directed toward the systematic organization of sequence-related attributes as gathered by a variety of algorithms, primary information from experimental data together with information compiled from the scientific literature. MIPS maintains automatically generated and manually annotated genome-specific databases, develops systematic classification schemes for the functional annotation of protein sequences and provides tools for the comprehensive analysis of protein sequences. This report updates the information on the yeast genome (CYGD), the Neurospora crassa genome (MNCDB), the database of complete cDNAs (German Human Genome Project, NGFN), the database of mammalian protein-protein interactions (MPPI), the database of FASTA homologies (SIMAP), and the interface for the fast retrieval of protein-associated information (QUIPOS). The Arabidopsis thaliana database, the rice database, the plant EST databases (MATDB, MOsDB, SPUTNIK), as well as the databases for the comprehensive set of genomes (PEDANT genomes) are described elsewhere in the 2003 and 2004 NAR database issues, respectively. All databases described, and the detailed descriptions of our projects can be accessed through the MIPS web server (http://mips.gsf.de).