Miracle-dz/UniFAR-FTUs huggingface dataset:
Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002

Predictive control of gene expression is an essential tool for developing synthetic biological systems. The current toolbox for controlling gene expression in cyanobacteria is a barrier to more in‐depth genetic analysis and manipulation. Towards relieving this bottleneck, this work describes the use of synthetic biology to construct an anhydrotetracycline‐based induction system and adapt a trans‐acting small RNA (sRNA) system for use in the cyanobacterium Synechococcus sp. strain PCC 7002. An anhydrotetracycline‐inducible promoter was developed to maximize intrinsic strength and dynamic range. The resulting construct, PEZtet, exhibited tight repression and a maximum 32‐fold induction upon addition of anhydrotetracycline. Additionally, a sRNA system based on the Escherichia coli IS10 RNA‐IN/OUT regulator was adapted for use in Synechococcus sp. strain PCC 7002. This system exhibited 70% attenuation of target gene expression, providing a demonstration of the use of sRNAs for differential gene expression in cyanobacteria. These systems were combined to produce an inducible sRNA system, which demonstrated 59% attenuation of target gene expression. Lastly, the role of Hfq, a critical component of sRNA systems in E. coli, was investigated. Genetic studies showed that the Hfq homolog in Synechococcus sp. strain PCC 7002 did not impact repression by the engineered sRNA system. In summary, this work describes new synthetic biology tools that can be applied to physiological studies, metabolic engineering, or sRNA platforms in Synechococcus sp. strain PCC 7002. Biotechnol. Bioeng. 2016;113: 424–432. © 2015 Wiley Periodicals, Inc.
Nongenomic steroid action: controversies, questions, and answers.

Steroids may exert their action in living cells by several ways: 1). the well-known genomic pathway, involving hormone binding to cytosolic (classic) receptors and subsequent modulation of gene expression followed by protein synthesis. 2). Alternatively, pathways are operating that do not act on the genome, therefore indicating nongenomic action. Although it is comparatively easy to confirm the nongenomic nature of a particular phenomenon observed, e.g., by using inhibitors of transcription or translation, considerable controversy exists about the identity of receptors that mediate these responses. Many different approaches have been employed to answer this question, including pharmacology, knock-out animals, and numerous biochemical studies. Evidence is presented for and against both the participation of classic receptors, or proteins closely related to them, as well as for the involvement of yet poorly understood, novel membrane steroid receptors. In addition, clinical implications for a wide array of nongenomic steroid actions are outlined.
A scientometric study of media literacy literature based on Scopus record through 2011

The present research is an attempt to describe the quantity and quality of publication trends of media literacy based on Scopus reports. The population under study was composed of 510 documents on media literacy published through 2011. The results were analyzed based on date of publication, type of document, language of the documents, source of publications, subject areas, authors and their affiliations, and the countries involved in developing the articles. Citation indicators formed the second phase of investigation in the present study. With a negligible amount of fluctuation, the number of publications on media literacy has increased steadily over the years, with the greatest number occurring in 2011. The analysis of data based on document types indicated that, of the 510 documents, 330 were articles (64.70%), 85 were reviews (16.66%), and 64 were conference papers (12.54%). 446 documents (84.45%) were published in English and the rest presented in other languages. The United States with 190 documents (37.25%), the United Kingdom with 43 documents (8.43%), and Australia with 14 documents (2.74%) were the most represented countries, with the most prolific authors known as Austin, Hobbs, and Cheung. Thematically, the greatest number of documents were produced in Social Sciences with 360 documents (70.57%), followed by Medicine with 92 documents (18.03%). The largest number of documents has been published in Comunicar followed by American Behavioral Scientist, and Simile. The most cited articles from 1996 up to 2011 included 22 documents on the H-index zone. Analysis of the results of H-index zone by subject categories indicated that most documents on h-index zone belonged to social sciences and Medicine areas. Psychology had the most influence on media literacy, as was shown by the ratio of most cited documents to total number of documents on media literacy in any of the subject categories. The results highlight the multidisciplinary nature of media literacy. As for the H-index zone, 16 documents (73.91%) belonged to the United States. Overall, the United States, with 190 (37.25%) publications (out of a total of 510), had an important role in enhancing the quantity and quality of the media literacy literature
Ability Matching and Occupational Choice

This paper develops and estimates an individual model of occupational choice and learning that allows for correlated learning across occupation-specific abilities. As an individual learns about their occupation-specific ability in one occupation, this experience will be broadly informative about their abilities in all occupations. Workers continually process their entire history of information, which they use to determine when to change careers, as well as which new career to go to. Endogenizing information in this manner has been computationally prohibitive in the past. I estimate the model in an innovative way using the Expectation and Maximization (EM) algorithm. The model is estimated on the National Longitudinal Survey of Youth 1997. The estimates suggest that both direct and indirect learning play an important role in early career wage growth, with those with the lowest levels of education achieving the largest increases.
Predictors of bone mineral density in african-american and caucasian college-aged women.

BACKGROUND Research regarding risk factors and prevalence of low bone mineral density (BMD) among African-American and Caucasian college-aged women are limited. The objective of this cross-sectional study was to determine if selected predictors of BMD in African-American and Caucasian college-aged women differ by race. METHODS A total of 101 local African-American (n=50) and Caucasian (n=51) females, ages 18 to 30 years, were in this study. All data were collected in the Bone Density and Body Composition Laboratory. BMD was measured using DXA technology. Race, family history of osteoporosis, BMI, current physical activity, osteoporosis knowledge, length of time on oral contraceptives, age at menarche and calcium intake were included in the multiple regression analyses with spinal and femoral BMD as dependent variables. RESULTS Overall, 38.6% had low spinal BMD and 7.9% had low femoral BMD. BMI (β=0.073, R(2) = . 148, P = . 001, 95% CI [0.030, 0.116]) and current physical activity (β=0.071, R(2) = . 148, P = . 017, 95% CI [0.013, 0.129]) were the only variables that were statistically significant in predicting spinal BMD. BMI (β=0.056, R(2) = . 13, P = . 010, 95% CI [0.014, 0.098]) and current physical activ-ity (β=0.078, R(2) = . 13, P = . 007, 95% CI [0.022, 0.134]) were also the only varia-bles that were statistically significant in predicting femoral BMD. Race was not a significant predictor of spinal or femoral BMD. CONCLUSION It is imperative for both African-American and Caucasian women to engage in osteoporosis-preventive behaviors.
Differential expression of voltage‐activated calcium currents in zebrafish retinal ganglion cells

We report a study on the characterization of voltage‐activated calcium currents (ICa) in retinal ganglion cells (RGCs) and the topographic distribution of RGCs that express different types of ICa in zebrafish retinas. In acutely isolated zebrafish RGCs, both high‐voltage‐activated (HVA; peak activation potential +7.4 ± 1.1 mV) and low‐voltage‐activated (LVA; peak activation potential –33.0 ± 1.2 mV) ICa were recorded. HVA ICa were recorded in all of the tested RGCs, whereas LVA ICa were recorded in approximately one‐third of the tested cells. In RGCs that expressed both HVA and LVA ICa, the two currents were readily separated by depolarizing the cell membrane to different voltages from different holding potentials. Among RGCs that expressed LVA ICa, some cells expressed large LVA ICa (up to 130 pA), whereas others expressed small LVA ICa (approximately 20 pA). RGCs that expressed large and small LVA ICa were designated as class I and class II cells, respectively, and RGCs that expressed only HVA ICa were designated as class III cells. The topographic distribution of cell classes was similar in various areas of the retina. In the nasal‐ventral retina, for example, class III cells outnumbered class I and class II cells by 10.8‐ and 2.6‐fold, respectively. In the temporal and dorsal retinas, the density of class III cells slightly decreased, whereas the density of class I and class II cells increased. The differential expression of ICa in RGCs may correlate with the development and function of the retina. © 2006 Wiley‐Liss, Inc.
Extraction of Building Heights from VHR SAR Imagery using an Iterative Simulation and Match Procedure

The new spaceborne very high resolution (VHR) SAR sensors onboard the TerraSAR-X and COSMO-SkyMED satellites have a spatial resolution of up to 1 meter. In VHR SAR data, features from individual urban structures (like buildings) can be identified in their characteristic settings in urban settlement patterns. In this paper, we present a novel methodology for the height estimation for generic man made structures from single power SAR data. The proposed approach is based on the definition of a hypothesis on the height of the building and on the simulation of a SAR image for that hypothesis. Then a matching procedure is applied between the estimated and the actual SAR images in order to validate the height assumption. The process is iterated for different initial height assumptions until the matching function is satisfied and thus the building height is estimated. The efficiency and the properties of the proposed method are demonstrated for the height estimation of flat- and gable roof buildings from a VHR airborne SAR scene and for a pyramid in Giza (Egypt) from VHR TerraSAR-X SPOT beam data.
Dynamic Impact Analysis Using Execution Profile Tracing

Impact analysis predicts and determines the parts of a software system that can be affected by changes of the system. Before or after such changes are made, impact analysis helps reduce the risk and costs caused by unwanted impact from changes. Traditional static impact analysis techniques, based on static system information, tend to produce imprecise results that are hardly useful. Dynamic impact analysis techniques are based on dynamic system behaviors, thus produce more precise and useful results. Existing dynamic impact analysis techniques impose various amounts of overhead costs in time and space, and produce impact sets of different degree of precision. In this paper, we propose a new dynamic impact analysis technique that is less expensive in both time and space than existing techniques, and produce safe and precise impact set relative to the dynamic information used in calculation
Teaching the Taboo: Courage and Imagination in the Classroom

It sounds good when knowing the teaching the taboo courage and imagination in the classroom second edition in this website. This is one of the books that many people looking for. In the past, many people ask about this book as their favourite book to read and collect. And now, we present hat you need quickly. It seems to be so happy to offer you this famous book. It will not become a unity of the way for you to get amazing benefits at all. But, it will serve something that will let you get the best time and moment to spend for reading the book.
Meaning, Discourse and Society

Introduction Part I. Meaning, the Mind and the Brain: 1. The cognitive turn 2. The long history of mind linguistics 3. What do we know about mental concepts? 4. Morphing theoretical semes into 'real' concepts 5. From mental representations to conceptual ontologies 6. What is meaning? 7. Where should we look for meaning? Part II. Discourse and Society: 8. Language as discourse 9. Society presupposes language, and language presupposes society 10. A closer look at oral societies 11. Differences between oral and literate societies 12. Empirical linguistics deals only with recorded language 13. Meaning, knowledge and the construction of reality 14. The language of the scientific experimental report 15. Diachronicity, intertextuality and hermeneutics 16. Meaning and the interpretation of a haiku Conclusion.
On the study of rain profile retrieval algorithm development: First results from airborne rain radar field campaign in China

This paper describes field campaign situations of first spaceborne rain radar in China, including related background introduction, instrument parameters, field campaign atmospheric condition and so on. What's more, based on the high precision Goddard Cumulus Ensemble (GCE) model, this paper focused on the calculation of two main rain parameter relations (k−Ze and R − Ze relations) for Ka band. Then, by using this filed campaign observation data and the rain parameter relations mentioned above, the rain attenuation corrections were done and rain profiles were retrieved. Finally, some rain-profiling retrieval results were shown. Comparing with the local weather situation, the retrieval results are reasonable.
Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley

The aims of this work was to characterise indigenous Saccharomyces cerevisiae strains in the naturally fermented juice of grape varieties Cabernet Sauvignon, Grenache, Tempranillo, Sauvignon Blanc and Verdejo used in the São Francisco River Valley, northeastern Brazil. In this study, 155 S. cerevisiae and 60 non-Saccharomyces yeasts were isolated and identified using physiological tests and sequencing of the D1/D2 domains of the large subunit of the rRNA gene. Among the non-Saccharomyces species, Rhodotorula mucilaginosa was the most common species, followed by Pichia kudriavzevii, Candida parapsilosis, Meyerozyma guilliermondii, Wickerhamomyces anomalus, Kloeckera apis, P. manshurica, C. orthopsilosis and C. zemplinina. The population counts of these yeasts ranged among 1.0 to 19 × 105 cfu/mL. A total of 155 isolates of S. cerevisiae were compared by mitochondrial DNA restriction analysis, and five molecular mitochondrial DNA restriction profiles were detected. Indigenous strains of S. cerevisiae isolated from grapes of the São Francisco Valley can be further tested as potential starters for wine production.
Experimental investigation of freewheeling current in indirect matrix converters when abnormal situation

In this paper, the normally-on type bidirectional switch, in which is comprised of normally-on and normally-off switching devices is proposed for one of a protection method of the Matrix Converter (MC). A diode clamp circuit or auxiliary protection circuits have to keep inductive load current loop in a MC utilized a bidirectional switch when the MC becomes gate block situation under abnormal conditions. We focus that the normally-on type switching devices like SiC-JFETs and GaN power devices becomes turn-on state, when zero gate bias voltage. These characteristic of normally-on type is used to replace diode clamp circuit in MC driver system. In this research, the experimentation based on indirect MC for Induction Motor (IM) driver system was carried out. The experimental result of IM driver shows that the proposed bidirectional switch can safely conduct a generated inductive load current. Under a variety of operation conditions of voltage, frequency, and load torque, the proposed method is investigated and evaluated.
Disjunctive datalog

We consider disjunctive Datalog, a powerful database query language based on disjunctive logic programming. Briefly, disjunctive Datalog is a variant of Datalog where disjunctions may appear in the rule heads; advanced versions also allow for negation in the bodies which can be handled according to a semantics for negation in disjunctive logic programming. In particular, we investigate three different semantics for disjunctive Datalog: the minimal model semantics the perfect model semantics, and the stable model semantics. For each of these semantics, the expressive power and complexity are studied. We show that the possibility variants of these semantics express the same set of queries. In fact, they precisely capture the complexity class P2 . Thus, unless the Polynomial Hierarchy collapses, disjunctive Datalog is more expressive that normal logic programming with negation. These results are not only of theoretical interest; we demonstrate that problems relevant in practice such as computing the optimal tour value in the Traveling Salesman Problem and eigenvector computations can be handled in disjunctive Datalog, but not Datalog with negation (unless the Polynomial Hierarchy collapses). In addition, we study modularity properties of disjunctive Datalog and investigate syntactic restrictions of the formalisms.
Genetic and Molecular Characterization of the Caenorhabditis elegans Spermatogenesis-Defective Gene spe-1 7

Two self-sterile mutations that define the spermatogenesis-defective gene spe-I 7 have been analyzed. These mutations affect unc-22 and fail to complement each other for both Unc-22 and spermatogenesis defects. Both of these mutations are deficiencies (hcDfl and hDfl3) that affect more than one transcription unit. Genomic DNA adjacent to and including the region deleted by the smaller deficiency (hcDfl) has been sequenced and four mRNAs (including unc-22) have been localized to this sequenced region. The three non unc-22 mRNAs are shown to be sex-specific: a 1.2-kb mRNA that can be detected in sperm-free hermaphrodites and 1.2- and 0.56-kb mRNAs found in males. hDfl3 deletes at least 55 kb of chromosome ZV, including all of unc-22, both male-specific mRNAs and at least part of the female-specific mRNA. hcDf1, which is approximately 15.6 kb, deletes only the 5‘ end of unc-22 and the gene that encodes the 0.56-kb male-specific mRNA. The common defect that apparently accounts for the defective sperm in hcDfl and hDfl3 homozygotes is deletion of the spe-I 7 gene, which encodes the 0.56-kb mRNA. Strains carrying two copies of either deletion are self-fertile when they are transgenic for any of four extrachromosomal array that include spe-I 7. We have sequenced two spe-17 cDNAs, and the deduced 142 amino acid protein sequence is highly charged and rich in serine and threonine, but shows no significant homology to any previously determined protein sequence.
Age as a moderator of attitude towards technology in the workplace: work motivation and overall job satisfaction

Given the prevalence of technology in the workplace, an understanding of employees' attitudes towards technology is essential. Such attitudes have been linked to such important issues as the successful implementation of new technologies in the workplace, employee intent to use technology, and the actual usage of technology by employees. As a result of the rapidly aging workforce, and because age has been linked to computer use and comfort, it is important to examine the relationship that may exist between age and attitudes towards technology. This study examines age as a moderator of 612 employees' attitudes towards technology in relation to work motivation (intrinsic and extrinsic) and overall job satisfaction. Further, given the technological socialisation of the Generation X (Gen X) versus the Baby Boomers, our sample comprised these two demographics. Hierarchical moderated multiple regression indicates age moderates the relationship between attitude towards technology and intrinsic motivation, extrinsic motivation, and to a lesser extent, overall job satisfaction. In each instance, older employees exhibit the strongest relationships with the outcome variables when possessing a high attitude towards technology. In contrast, older employees exhibit the weakest relationships when possessing a low attitude towards technology. These results are supportive of the moderating effect of age on attitude towards technology. Lastly, implications and directions for future research are discussed.
A Two-Way Communication between Microglial Cells and Angiogenic Sprouts Regulates Angiogenesis in Aortic Ring Cultures

Background Myeloid cells have been associated with physiological and pathological angiogenesis, but their exact functions in these processes remain poorly defined. Monocyte-derived tissue macrophages of the CNS, or microglial cells, invade the mammalian retina before it becomes vascularized. Recent studies correlate the presence of microglia in the developing CNS with vascular network formation, but it is not clear whether the effect is directly caused by microglia and their contact with the endothelium. Methodology/Principal Findings We combined in vivo studies of the developing mouse retina with in vitro studies using the aortic ring model to address the role of microglia in developmental angiogenesis. Our in vivo analyses are consistent with previous findings that microglia are present at sites of endothelial tip-cell anastomosis, and genetic ablation of microglia caused a sparser vascular network associated with reduced number of filopodia-bearing sprouts. Addition of microglia in the aortic ring model was sufficient to stimulate vessel sprouting. The effect was independent of physical contact between microglia and endothelial cells, and could be partly mimicked using microglial cell-conditioned medium. Addition of VEGF-A promoted angiogenic sprouts of different morphology in comparison with the microglial cells, and inhibition of VEGF-A did not affect the microglia-induced angiogenic response, arguing that the proangiogenic factor(s) released by microglia is distinct from VEGF-A. Finally, microglia exhibited oriented migration towards the vessels in the aortic ring cultures. Conclusions/Significance Microglia stimulate vessel sprouting in the aortic ring cultures via a soluble microglial-derived product(s), rather than direct contact with endothelial cells. The observed migration of microglia towards the growing sprouts suggests that their position near endothelial tip-cells could result from attractive cues secreted by the vessels. Our data reveals a two-way communication between microglia and vessels that depends on soluble factors and should extend the understanding of how microglia promote vascular network formation.
Non‐invasive online detection of microbial lysine formation in stirred tank bioreactors by using calorespirometry

Non‐invasive methods for online monitoring of biotechnological processes without compromising the integrity of the reactor system are very important to generate continuous data. Even though calorimetry has been used in conventional biochemical analysis for decades, it has not yet been specifically applied for online detection of product formation at technical scale. Thus, this article demonstrates a calorespirometric method for online detection of microbial lysine formation in stirred tank bioreactors. The respective heat generation of two bacterial strains, Corynebacterium glutamicum ATCC 13032 (wild‐type) and C. glutamicum DM1730 (lysine producer), was compared with the O2‐consumption in order to determine whether lysine was formed. As validation of the proposed calorespirometric method, the online results agreed well with the offline measured data. This study has proven that calorespirometry is a viable non‐invasive technique to detect product formation at any time point. Biotechnol. Bioeng. 2013; 110: 1386–1395. © 2012 Wiley Periodicals, Inc.