Miracle-dz/UniFAR-FTUs huggingface dataset:
THE FITNESS EFFECTS OF OUTCROSSING IN CALYLOPHUS SERRULATUS, A PERMANENT TRANSLOCATION HETEROZYGOTE

Abstract Small and relatively isolated populations that occupy fragmented habitat are at risk of local extinction. However, fitness consequences of fragmentation related to mating distance, such as inbreeding depression following increased self- and near-neighbor mating, may not follow standard expectations in species with specialized genetic systems. We investigated the effect of mating distance on progeny fitness in Calylophus serrulatus, a primarily autogamous, permanent translocation heterozygote that is restricted to prairie fragments in the North American tallgrass prairie region. We pollinated flowers by hand in the field with pollen sampled at various distances from the maternal parent within and between three populations in southeastern Minnesota. We raised the progeny in a greenhouse and measured fitness-related characters. Because their genetic system prevents loss of heterozygosity throughout much of the genome, regardless of inbreeding, permanent translocation heterozygotes are not expected to exhibit inbreeding depression. Consistent with this expectation, in no case did progeny of self matings suffer significantly reduced mean fitness compared to progeny from crosses between plants. Crosses between plants in the two closely situated (2 km) populations yielded progeny with fitness intermediate to their parents, but crosses between each of those populations and the more distant (20 km) population yielded progeny with reduced fitness, suggesting outbreeding depression at this largest spatial scale. Similarly, fitness of self-pollinated progeny and progeny from “near” crosses (<2 m) within populations tended to be higher than “mid” (10–25 m) and “far” (>35 m) cross-progeny fitness. Under the current conditions of fragmentation, it seems likely that the distant matings that produce outbreeding depression are rare. It appears that mean fitness in this species is maintained in the context of severe fragmentation of its populations, largely because of its genetic system.
Quantification of Protein A-Gold Staining for Peroxisomal Enzymes by Confocal Laser Scanning Microscopy

The protein A-gold technique has been widely applied for visual localization and quantification of various antigens by electron microscopy. Observation of specimens stained by the protein A-gold technique with conventional light microscopy is difficult because of insufficient sensitivity of the staining. Light microscopic visualization and quantification of the reaction products were attempted employing a confocal laser scanning microscope (CLSM). Liver tissues of normal and peroxisome proliferator-treated rats were fixed and embedded in Lowicryl K4M resin. Ultrathin and thin sections were stained for catalase and a peroxisome-specific β-oxidation enzyme by the protein A-gold technique. Ultrathin sections were observed by electron microscopy and the labeling density for each enzyme was analyzed with an image analyzer. Thin sections were observed with a CLSM in the reflection mode and the intensity of the light reflection was analyzed under the same conditions for all specimens. A comparison of these two observation procedures was also attempted using liver tissues stained with various concentrations of the antibody for catalase. The intensity of the reflection for each, as observed by CLSM, correlated well with the labeling density observed by electron microscopy. CLSM made it possible to quantify and to directly observe protein A-gold staining at the light microscopic level.
Object-based image analysis for urban land cover classification in the city of Campinas - SP, Brazil

Classifiers that make use of pixel-by-pixel approaches are limited in the high spatial and radiometric resolution of urban areas, that happens mostly because of the similarity between the target's spectral response like ceramic roofs and bare soil. Because of that, the literature favors approaches that make use of object-oriented analysis for image interpretation, those approaches make a better use of the high spatial resolution and do not use only the target spectral response. Assuming that the object-oriented analysis is a favorable approach to be employed for intra-urban image classification, this paper will assess the results of such approach through an implementation of it in an urbanized area from the city of Campinas (Brazil), which has a size close to twelve square kilometers. Making use of the fusion of high spatial resolution image from Worldview-2 sensor and it's panchromatic band, the experiments were performed with the use of eCognition Developer 8 as the segmentation platform, and the classification being based on a decision tree generated by J48 (C4.5) algorithm on the software WEKA. This work also assess which approach best suits the experiment needs, being an optimal attribute selection achieved through a Wrapper filter, with a final kappa statistic of 0.9425.
Temporal integration and multiple looks.

The decrease in detection and discrimination thresholds with increases in signal duration has often been taken to indicate that a process of relatively long-term temporal integration occurs in hearing. Two experiments are reported that suggest that no such process occurs. The first experiment is similar to the two-pulse experiment reported by Zwislocki [J. Zwislocki, J. Acoust. Soc. Am. 32, 1046-1059 (1960)] in which the threshold in quiet for a pair of brief pulses is measured as a function of the temporal separation between them. Our data indicate that power integration occurs only for separations less than approximately 5 ms. For separations larger than 5-10 ms, thresholds do not change with separation and the pulses appear to be processed independently. In the second experiment, brief 1-kHz tone pulses separated by 100 ms are presented during gaps in a wideband noise. The threshold for a pair of pulses is lower than that for either pulse presented alone, indicating that some type of "integration" occurs. However, the threshold for the pulse pair is not affected by changes in the level of the noise during the interval between the pulses. These data are inconsistent with the classical view of temporal integration that involves long-term integration. They are consistent with the notion that the input is sampled at a fairly high rate and that these samples or "looks" are stored in memory and can be accessed and processed selectively. This multiple-look model can account for the data from the present experiment and also can account for the data on temporal integration for tones and noise. (ABSTRACT TRUNCATED AT 250 WORDS)
Single amino acid substitution in constant region 1 or 4 of gp120 causes the phenotype of a human immunodeficiency virus type 1 variant with mutations in hypervariable regions 1 and 2 to revert

The second major cysteine loop of human immunodeficiency virus type 1 envelope glycoprotein gp120 contains 5 to 11 consensus N-linked glycosylation sites, which is disproportionately higher than the number of such sites found in other regions of gp120. Amino acid substitutions introduced at three of six N-linked glycosylation sites in this region of an infectious molecular clone, HXB2, resulted in severe impairment of virus infectivity. Isolation and genetic characterization of a revertant of this mutant revealed an isoleucine-for-valine substitution at position 84 in constant region 1 and an isoleucine-for-methionine substitution at position 434 in constant region 4. Further mutational analysis indicated that either isoleucine substitution was sufficient to confer the revertant phenotype. These findings demonstrate that V1/V2 not only functionally interacts with C4, as previously reported, but also interacts with C1. The observation that compensatory changes do not involve regeneration of N-linked glycosylation sites in the second major cysteine loop suggests that replication of human immunodeficiency virus type 1 in vitro is independent of the presence of a disproportionate number of N-linked glycosylation sites within this loop.
Follicular lymphoma at relapse after rituximab containing regimens: comparison of time to event intervals prior to and after 90Y‐ibritumomab‐tiuxetan

Radioimmunotherapies with Zevalin® (RIT‐Z) showed encouraging results in patients with relapsed/refractory follicular lymphoma (FL), leading frequently to failure‐free intervals longer than those achieved by the last previous therapy. We compared time‐to‐event variables obtained before and after RIT‐Z in patients with relapsed FL, previously exposed to rituximab. All patients with relapsed non‐transformed, non‐refractory, non‐rituximab‐naïve FL who have been treated with RIT‐Z in two different centres in Europe were included. Staging and response were assessed by contrast‐enhanced CT in all patients; PET/CT was performed according to local availability. Event‐free survival (EFS) and time to next treatment (TTNT) following the last previous therapy and after RIT‐Z were compared. Pre‐therapy characteristics were tested in univariate analyses for prediction of outcomes. A description of the patterns of relapse was also provided. Among 70 patients treated, only 16 fulfilled the inclusion criteria. They were treated with a median of 3 prior lines of chemo‐immunotherapies, including a median of 2 rituximab‐containing regimens; 6 patients had undergone myeloablative chemotherapy with autologous stem cell rescue (ASCT). Overall response rates were 10 (62%) CR/CRu, 3 (19%) PR and 3 (19%) PD; response rates were similar in patients with prior ASCT. After RIT‐Z only few patients obtained EFS and TTNT longer than after the last previous therapy. All four patients receiving rituximab maintenance were without progression 12 months after RIT‐Z. Relapses occurred in both previously and newly involved sites; a significant association was found between the number of pathologic sites involved prior to RIT‐Z and subsequent TTNT. Despite the excellent response rate, the duration of response was shorter than the previous one confirming the known trend of relapses to occur earlier after subsequent treatments. Rituximab maintenance after RIT‐Z showed encouraging results in terms of prolonging EFS, warranting further studies. Copyright © 2010 John Wiley & Sons, Ltd.
Isolation and characterization of a new mutant human cell line unresponsive to alpha and beta interferons

Previously we described human cell line 2fTGH, in which expression of guanine phosphoribosyltransferase is tightly controlled by the upstream region of interferon (IFN)-stimulated human gene 6-16. After mutagenesis of 2fTGH and selection with 6-thioguanine and IFN-alpha, we isolated 11.1, a recessive mutant that does not respond to IFN-alpha. We now describe U2, a second recessive mutant, selected similarly, that complements 11.1. U2 had no response to IFN-alpha or IFN-beta, and its response to IFN-gamma was partially defective. Although many genes did respond to IFN-gamma in U2, the 9-27 gene did not and the antiviral response of U2 cells to IFN-gamma was greatly reduced. Band shift assays showed that none of the transcription factors normally induced in 2fTGH cells by IFN-alpha (E and M) or IFN-gamma (G) were induced in U2. However, extracts of untreated U2 cells gave rise to a novel band that was increased by treatment with IFN-gamma but not IFN-alpha. Band shift complementation assays revealed that untreated and IFN-gamma-treated U2 cells lack the functional E gamma subunit of transcription factor E and that IFN-alpha-treated U2 cells do contain the functional E alpha subunit.
Improving comprehension in adolescents with severe receptive language impairments: a randomized control trial of intervention for coordinating conjunctions.

BACKGROUND Little evidence exists for the effectiveness of therapy for children with receptive language difficulties, particularly those whose difficulties are severe and persistent. AIMS To establish the effectiveness of explicit speech and language therapy with visual support for secondary school-aged children with language impairments focusing on comprehension of coordinating conjunctions in a randomized control trial with an assessor blind to group status. METHODS & PROCEDURES Fourteen participants (aged 11;3-16;1) with severe RELI (mean standard scores: CELF4 ELS = 48, CELF4 RLS = 53 and TROG-2 = 57), but higher non-verbal (Matrices = 83) and visual perceptual skills (Test of Visual Perceptual Skills (TVPS) = 86) were randomly assigned to two groups: therapy versus waiting controls. In Phase 1, the therapy group received eight 30-min individual sessions of explicit teaching with visual support (Shape Coding) with their usual SLT. In Phase 2, the waiting controls received the same therapy. The participants' comprehension was tested pre-, post-Phase 1 and post-Phase 2 therapy on (1) a specific test of the targeted conjunctions, (2) the TROG-2 and (3) a test of passives. OUTCOMES & RESULTS After Phase 1, the therapy group showed significantly more progress than the waiting controls on the targeted conjunctions (d = 1.6) and overall TROG-2 standard score (d = 1.4). The two groups did not differ on the passives test. After Phase 2, the waiting controls made similar progress to those in the original therapy group, who maintained their previous progress. Neither group showed progress on passives. When the two groups were combined, significant progress was found on the specific conjunctions (d = 1.3) and TROG-2 raw (d = 1.1) and standard scores (d = 0.9). Correlations showed no measures taken (including Matrices and TVPS) correlated significantly with progress on the targeted conjunctions or the TROG-2. CONCLUSIONS & IMPLICATIONS Four hours of Shape Coding therapy led to significant gains on comprehension of coordinating conjunctions which were maintained after 4 months. Given the significant progress at a group level and the lack of reliable predictors of progress, this approach could be offered to other children with similar difficulties to the participants. However, the intervention was delivered one-to-one by speech and language therapists, thus the effectiveness of this therapy method with other methods of delivery remains to be evaluated.
AF Wireless Relay Network Analysis under Receiver Power Constraint

This paper presents both cooperative and noncooperative amplify-and-forward (AF) relay networks consisting of a one-source-one-destination pair and N-relays under receiver power constraint (RPC) at the destination. The optimal AF relay amplifying matrices for the relays by minimizing the mean square error are analytically derived. This paper also shows that the performance of the optimum cooperative relay network is identical to that of the optimum noncooperative network, as long as the same receiver power constraint is applied. The purpose of the RPC is to reduce the interference level for users located in neighboring cells. Finally, using the derived optimal relay amplifying matrices, the achievable rate, the pairwise error probability, and the outage probability are investigated.
Feasibility of applying the psychosocial intervention STrAtegies for RelaTives to family caregivers of patients with dementia: a case report

As the number of people with dementia in Japan continues to rise, family caregivers are experiencing an increasing care burden. Previous research has shown that this care burden can affect family caregivers' physical and mental health. Therefore, providing support for caregivers of family members with dementia has become an urgent issue in the country. The purpose of this study was to investigate the feasibility of applying STrAtegies for RelaTives (START), a psychosocial intervention programme developed in the UK to improve caregivers' moods and quality of life, to Japanese family caregivers. Specifically, in this paper, we provide a detailed description of the application of the Japanese version of START to a female caregiver. Our investigation found that the Japanese version of START is a feasible option for alleviating the mental and physical burden on family caregivers of patients with dementia. This result provides preliminary support for the wider application of START in Japan, as it can decrease care burden and improve the daily lives of caregivers of people with dementia. It also supports the development and implementation of other systems that can provide similar services for other caregivers.
Bilateral teleoperation under time-varying communication delay

Several approaches based on wave variables have already been proposed for bilateral teleoperation control under time-varying delay. However, these approaches are too conservative for keeping the system passivity or for estimating the amount of virtually constant time delay. These conservative approaches result in too much degradation of maneuverability from the constant time delay situations. We propose a novel control scheme that is also based on wave variables, but minimizes the performance degradation due to the fluctuation of the communication time delay. The proposed method is simple and easy to implement. We conducted some simulation studies with a one DOF system. Simulation results show the validity of the proposed scheme.
LAWC: Optimizing Write Cache Using Layout-Aware I/O Scheduling for All Flash Storage

Flash memory-based SSD-RAIDs are swiftly replacing conventional hard disk drives by exhibiting improved performance and stability, especially in I/O-intensive environments. However, the variations in latency and throughput occurring due to uncoordinated internal garbage collection cripples further boosting of performance. In addition, the unwanted variations in each SSD can influence the overall performance of the entire flash storage adversely. This performance bottleneck can be essentially reduced by an internal write cache in the RAID controller designed prudently by considering the crucial device characteristics. The state-of-the-art cache write for the RAID controller fails to incorporate device characteristics of flash memory-based SSDs and mitigates the performance gain. In this paper, we propose a novel cache design namely Layout-Aware Write Cache (LAWC) to overcome the performance barrier inculcated by independent garbage collections. LAWC implements (i) improved I/O scheduling for logically partitioned write caches, (ii) a destage write synchronization mechanism to allow individual write caches to flush write blocks into the SSD array in a coordinated manner, and (iii) a two-level hybrid cache algorithm utilizing small front level cache for the improved write cache efficiency. LAWC shows significant reduction in response time by 82.39 percent on RAID-0 and 68.51 percent on RAID-5 types of SSDs when compared with state-of-the-art write cache algorithms.
Urban Air Pollution and Emergency Visits for Respiratory Complaints in Pisa, Italy

Emergency room visits for respiratory complaints, considered an indicator of deterioration of respiratory health, have been positively correlated to traffic-related air pollution. This is an ecological study to evaluate the association between daily levels of urban air pollutants and emergency visits for respiratory complaints, in children and the elderly residing in Pisa, Italy. Daily records of emergency department visits for respiratory complaints were selected from computerized registries, and paper medical reports were identified. Pollutant daily means were computed from hourly values obtained from the public network. Poisson regression, allowing for overdispersion and autocorrelation, was used to evaluate the percent change in daily visits associated with variations of air pollution measures. Among children, an increase in emergency visits of 10% (95% CL: 2.3; 18.2) was associated with a 10-μg/m3 increase in PM10 concentrations of the previous day and an increase of 11.8% (95% CL:1.4; 23.3) was associated with an analogous increment of NO2 during the 2 previous days. Among elderly the same increment of PM10 level during the 2 previous days was correlated with a 8.5% (95% CL: 1.5; 16.1) increase in the risk of recurring emergency department visits for respiratory complaints; this risk increased to 26.5% (95% CL: 3.4; 54.8) if the daily level of CO over the 4 previous days increased by 1 mg/m3. Evidence of morbidity related to short- and long-term air pollution exposures provided in this small study and larger studies conducted in other countries suggests the need for actions to improve air quality in any urban context.
The RNA/DNA‐binding protein PSF relocates to cell membrane and contributes cells' sensitivity to antitumor drug, doxorubicin

Cell surface proteins play an important role in multidrug resistance (MDR). However, the identification involving chemoresistant features for cell surface proteins is a challenge. To identify potential cell membrane markers in hematologic cancer MDR, we used a cell‐ and antibody‐based strategy of subtractive immunization coupled with cell surface comparative screening of leukemia cell lines from sensitive HL60 and resistant HL60/DOX cells. Fifty one antibodies that recognized the cell surface proteins expressed differently between the two cell lines were generated. One of them, the McAb‐5D12 not only recognizes its antigen but also block its function. Comparative analysis of immunofluorescence, flow cytometry, and mass spectrum analysis validated that the membrane antigen of McAb‐5D12 is a nucleoprotein—polypyrimidine tract binding protein associated splicing factor, PSF. Our results identified that PSF overexpressed on the membrane of sensitive cells compared with resistant cells and its relocation from the nuclear to the cell surface was common in hematological malignancy cell lines and marrow of leukemia patients. Furthermore, we found that cell surface PSF contributed to cell sensitivity by inhibiting cell proliferation. The results represent a novel and potentially useful biomarker for MDR prediction. The strategy enables the correlation of expression levels and functions of cell surface protein with some cell‐drug response traits by using antibodies. © 2013 International Society for Advancement of Cytometry
A randomized, controlled trial on the effect of non-surgical periodontal therapy in patients with type 2 diabetes. Part I: effect on periodontal status and glycaemic control.

AIM the purpose of the present study was to assess the effect of non-surgical periodontal therapy on glycaemic control of type 2 diabetes patients with moderate-to-severe periodontitis. MATERIALS AND METHODS this was a randomized, controlled clinical trial of patients with type 2 diabetes. A total of 60 patients with moderate-to-severe periodontal disease were assigned to either a periodontal treatment arm, consisting of scaling and root planing (intervention group [IG]), or a delayed treatment arm that received periodontal care after 6 months (control group [CG]). Periodontal parameters and glycosylated haemoglobin (A1C) were evaluated at 1, 3 and 6 months. RESULTS all periodontal parameters improved significantly in the IG. A1C levels decreased statistically significantly more in the IG versus the CG (0.72%versus 0.13%; p<0.01) independently of other confounders. CONCLUSIONS this study provides evidence that periodontal treatment contributes to improved glycaemic control in type 2 diabetes mellitus patients. Larger controlled trials are needed to confirm if this finding is generalizable to other populations of patients with type 2 diabetes.