Miracle-dz/UniFAR-FTUs huggingface dataset:
Oligosaccharide analysis by mass spectrometry: a review of recent developments.

Carbohydrates are central players in a number of important biological processes including cell signaling, cell adhesion, and the regulation of biochemical pathways. Unlike nucleic acids and proteins, the biosynthesis of carbohydrates is not template-driven. They occur in nature as heterogeneous mixtures, often of high complexity. There is no method for amplifying the amount of a carbohydrate analogous to overexpression for proteins or polymerase chain reaction for nucleic acids, and so carbohydrate analysis is typically limited to what can be obtained from natural sources, thus the researcher must cope with small quantities of heterogeneous material. Mass spectrometry has high sensitivity and is tolerant of mixtures, and is a natural choice for the analysis of this class of molecules. Compared to advances in protein analysis, progress in the application of mass spectrometry to carbohydrates has evolved somewhat slowly, principally because carbohydrates are a more challenging set of targets for structural characterization. In contrast to proteins, there is no database containing an inclusive and closed set of sequences representing all possible carbohydrate structures. The characterization of carbohydrates relies upon obtaining the full details of structure from the mass spectrum. Subtle differences due to isomerism or chirality can produce molecules with very different biological activities, making complete structural analysis even more demanding. Mass spectrometry methodologies and technologies for biomolecule analysis continue to rapidly evolve and improve, and these developments have benefited carbohydrate analysis. These developments include approaches for improved ionization, new and improved methods of ion activation, advances in chromatographic separations of carbohydrates, the hybridization of ion mobility and mass spectrometry, and better software for data collection and interpretation. It thus seems timely to examine how these developments affect carbohydrate analysis. This review covers developments in the application of mass spectrometry to the analysis of carbohydrates, with an emphasis on work that has occurred from January 2011 through October 2013. The coverage is not mean to be exhaustive, but rather focuses on significant developments that, in the opinion of the authors, have advanced the field.
Extended Release Quetiapine Fumarate Monotherapy for Major Depressive Disorder: Results of a Double-Blind, Randomized, Placebo-Controlled Study

Abstract Introduction: Once-daily extended release quetiapine fumarate (quetiapine XR) monotherapy was evaluated in major depressive disorder (MDD). Method: This was an 8-week (6-week randomized-phase; 2-week drug-discontinuation/tapering phase), double-blind, parallel-group, placebo-controlled study. The primary outcome measure was Montgomery-Åsberg Depression Rating Scale (MADRS) total score randomization-to-Week 6 change. Other assessments included the Hamilton Rating Scale for Depression, the Hamilton Rating Scale for Anxiety, and adverse events (AEs). Results: 723 patients were randomized: 182, 178, 179, and 184 to quetiapine XR 50, 150, 300 mg/day, and placebo, respectively. At Week 6, significant reductions occurred in MADRS score with quetiapine XR 50 mg/day (−13.56; P<.05), 150 mg/day (−14.50; P<.01) and 300 mg/day (−14.18; P<.01) versus placebo (−11.07); at Day 4, reductions for quetiapine XR (titrated to 50 or 150 mg/day according to dose group) versus placebo (−2.9) were: −4.7 (P<.01), −5.2 (P<.001), and −5.1 (P<.001), respectively. At endpoint, MADRS response (≥50% reduction in score) was 42.7% (P<.01), 51.2% (P<.001), and 44.9% (P≤.001) for quetiapine XR 50, 150, and 300 mg/day, respectively; 30.3% for placebo. Overall, quetiapine XR 150 mg/day provided consistently more positive secondary efficacy results than 50 mg/day and 300 mg/day versus placebo. The most common AEs in quetiapine XR-treated patients were dry mouth, sedation, somnolence, headache, and dizziness. Conclusion: In patients with MDD, quetiapine XR monotherapy (50/150/300 mg/day) is effective in reducing depressive symptoms, with improvement from Day 4 onwards. Safety and tolerability were consistent with the known profile of quetiapine.
Human platelets express OATP 2 B 1 , an uptake transporter for atorvastatin

Department of Pharmacology, Research Center of Pharmacology and Experimental Therapeutics (J.N., G.J., M.G., S.B., S.O., K.S., W.S., D.R., H.K.K.), Department of Immunology and Transfusion Medicine (U.S., A.G.), Ernst-Moritz-Arndt-University, Greifswald, Germany and Department of Surgery, and The Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA (H.S.), Division of Membrane Transport and Drug Targeting, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan (S.O., H.K., J.K., T.T.) DMD Fast Forward. Published on February 23, 2009 as doi:10.1124/dmd.108. 024570
A dual band omni-directional antenna for WAVE and Wi-Fi

Vehicles of today are increasingly being networked via various available networking technologies. IEEE 802.11p advocates Vehicle-to-Vehicle and Vehicle-to-Infrastructure communication via Wireless Access in Vehicular Environments (WAVE) between vehicles in the frequency range of 5.9 GHz. Also, IEEE 802.11j proposes the usage of 4.9 GHz frequency range for Wi-Fi. This paper proposes a dual band antenna that is capable of operating in both the WAVE and Wi-Fi bands. This proposed antenna is expected to be simple, easy-to-produce and inexpensive; it can be a cost-effective alternative to use of multiple directional antennas for vehicles. The choice of microstrip patch antenna technology with defected ground structure (DGS) was driven by cost considerations and ease of bulk manufacturing. This omni-directional antenna is expected to be fitted in a central location in the vehicle to avoid requirement of two or more directional antennas. The proposed antenna is characterized by popular antenna design software Ansoft HFSS.
Regulation of the stability of plant disease resistance proteins

The Arabidopsis protein RPM1 activates disease resistance in response to Pseudomonas syringae proteins targeted to the inside of the host cell via the bacterial type III delivery system. We demonstrate that specific mutations in the ATP-binding domain of a single Arabidopsis cytosolic HSP90 isoform compromise RPM1 function. These mutations do not affect the function of related disease resistance proteins. RPM1 associates with HSP90 in plant cells The Arabidopsis proteins RAR1 and SGT1 are required for the action of many R proteins, and display some structural similarity to HSP90 co-chaperones. Each
Eleven-Year Follow-Up of a Prospective Study of Zirconia Implant Abutments Supporting Single All-Ceramic Crowns in Anterior and Premolar Regions.

BACKGROUND Clinical studies on zirconia abutments report very good survival rates and biological and technical results, but few have an observation period of more than 5 years. PURPOSE The aim of this study was to assess the long-term performance of customized zirconia implant abutments supporting all-ceramic crowns. MATERIALS AND METHODS Twenty-seven patients receiving 54 single implants were included (25 incisors, 14 canines, 15 premolars in both jaws). Yttria-stabilized zirconia abutments were screwed to the implants with a defined torque. All-ceramic crowns were adhesively cemented onto the abutments. The implants, abutments, and crowns were clinically and radiographically examined after 11 years of use. Modified United States Public Health Service (USPHS) criteria were used to assess technical outcomes: fracture of abutment/crown framework/veneering ceramic, loosening of abutment screw/crown, marginal adaptation, anatomical form, occlusal wear, and abutment fit. The biological parameters were pocket probing depth, plaque control record, bleeding on probing, papilla index, and gingival/mucosal recession at implants and neighboring natural teeth. The cumulative success rate of abutments and crowns was calculated by the Kaplan-Meier method. The results of the USPHS criteria were analyzed descriptively. RESULTS Sixteen patients with 31 zirconia abutments were examined at 11.3 (±0.9) years after implantation. No abutment or crown was lost. The cumulative success rate was 96.3% for abutments and 90.7% for crowns. Two abutment screws loosened, and three crowns exhibited minor chipping. There were no biological complications. CONCLUSIONS Customized zirconia single implant abutments exhibited excellent long-term outcomes in anterior and premolar regions.
No association between common variants in glyoxalase 1 and autism spectrum disorders

The autism spectrum disorders (ASDs) are complex diseases with a strong genetic component. Numerous candidate gene studies have tested association between various functional and positional candidate genes and autism, but no common variation predisposing for autism has been identified to date. It has been previously proposed, that glyoxalase 1 (GLO1) might be involved in the pathogenesis of autism as GLO1 protein polarity was significantly changed in the brains of autism patients compared to controls. GLO1 harbors a functional polymorphism that affects the polarity and the enzymatic activity of the protein. In the same study, this polymorphism showed a suggestive association to autism. To investigate whether common variants in GLO1 predispose to autism in the Finnish population, we have genotyped six polymorphisms in GLO1 in families with more than 230 individuals affected with ASDs and carried out both linkage and association analyses. We did not observe significant linkage or association between any SNP and ASDs. Therefore, we suggest that common variants in GLO1 are not significant susceptibility factors for ASDs in the Finnish population. © 2007 Wiley‐Liss, Inc.
IT Adoption: HealthCare Metrics Tracking

Continuous improvement on core metrics is a goal of any organization. Progress toward these goals occurs over multiple time-periods and process changes and technology adoptions are intended to influence these metrics. Hospitals have been investing in technology for over two decades to improve on patient quality outcomes. This research examines how core patient safety and mortality metrics are impacted by investments in Electronic Medical Record (EMR)systems. The improvements in composite indicators are modeled as latent growth curves with adjustments based on the EMR stage for the individual hospitals. Differing results are identified based on whether the indicators are tracking medical conditions, surgical procedures or composites of all rates tracked. The latent growth model technique shows the EMR stage to be a significant predictor of reductions in hospital mortality rates.
Features of an alternative hemodialysis method using a hemoconcentrator during cardiopulmonary bypass surgeries

Purpose: This study clarified the features of a hemoconcentrator-based, alternative hemodialysis (ALTHD) method that improves the speed of serum potassium (K+) concentration adjustments, compared with dilutional ultrafiltration (DUF), during cardiopulmonary bypasses. Methods: Standardized bovine blood was recirculated (300 ml/min) through an in vitro hemoconcentrator circuit; hematocrit, K+ and glucose levels were measured at 5–20 min after DUF or ALTHD. We evaluated DUF at dialysis speeds of 50–250 ml/min and ALTHD at speeds of 50–1000 ml/min. Results: ALTHD rapidly corrected K+ and glucose concentrations at speeds up to 800 ml/min. ALTHD took 8.9 min to reach a K+ level of 4.5 mmol/L, faster than DUF (12.8 min). The ALTHD efficiency curves plateaued at 600 ml/min. Conclusion: ALTHD allowed faster adjustment of electrolyte levels, with peak efficiency at 600 ml/min. ALTHD has possible clinical application if available for potential use during all cardiopulmonary bypass surgeries involving extracorporeal circulation.
An Ultra-Wideband Positioning Demonstrator Using Audio Signals

UWB technology has been recognized as an ideal candidate to provide positioning information in indoor environments. However, demonstrators useful to test UWB positioning algorithms are still difficult to realize due to the hardware requirements of this technology and the scarceness of components available in the market. For this reason, the use of an acoustic demonstrator is proposed. By scaling the frequencies with a specific factor, it is possible to obtain the same wavelengths for the audio signals as for the radio signals. In this way, similar interactions with the environment can be observed. A validation of this technique is provided, by analyzing and comparing the large scale statistics and the RMS delay spread of the channel impulse response obtained with the two types of signals, and the similarities and differences are underlined. Finally, first results on the TOA estimation accuracy for different propagation conditions and on the positioning performance achievable using audio signals are shown. With the proposed audio demonstrator an accuracy at centimeter level can be achieved for LOS propagation.
A Workload Selection Procedure for the Åstrand-Ryhming Test for Women

The Åstrand-Ryhming Submaximal Bicycle Test is an accurate and widely used test to estimate maximal oxygen consumption. This test requires a participant to maintain a workload over a 6-min. period. The issue facing many employing this protocol is the identification of an appropriate workload for the cycling regimen. The present study is designed to identify a method for determining an appropriate workload for female participants. A prior study designed a workload selection procedure specifically for men; however, the selection procedure designed for women in this study was better able to elicit a final heart rate (average of minutes five and six) between 165–170 bpm compared to the original Åstrand-Ryhming procedure.
A triple-mode ring dielectric resonator band-pass filter using substrate integrated waveguide (SIW)

This paper presents a design of triple mode ring dielectric resonator band-pass filter. The first mode TM010 and the second degenerated modes TM110 were excited. Instead of using conventional metal enclosures, substrate integrated waveguide (SIW) was used for easily integrating with other microwave circuits. Analytic equations were derived to determine the resonant frequencies and the quality factor of the ring dielectric resonator filter. It has been found that the calculated results agreed with simulated ones by using EM software. To develop a triple mode ring dielectric resonator filter, novel input and output structures were realized. The measured insertion of the filter is about 0.6dB with 3-dB fractional bandwidth of 9.8% obtained at operating frequency 2.1GHz. This proposed filter configuration effectively reduced the cost of assembly and the difficulty of integration associated with other circuits. The concept is very attractive for using in low cost, low insertion loss and high Q production of wireless filter application.
EWMA smoothing and Bayesian spatial modeling for health surveillance

In this paper a novel method for the monitoring of disease maps over time in a surveillance setting is described. The approach relies upon the use of a spatial model that is fitted to current spatial data and is smoothed with historical spatial estimates. The method of smoothing is a vector exponentially weighted moving average procedure. A simulation study with a range of scenarios is presented and finally a case study of monitoring infectious disease spread is presented. Copyright © 2008 John Wiley & Sons, Ltd.
Antioxidant responses to an acute ultra-endurance exercise: impact on DNA stability and indications for an increased need for nutritive antioxidants in the early recovery phase

Antioxidant requirements have neither been defined for endurance nor been defined for ultra-endurance athletes. To verify whether an acute bout of ultra-endurance exercise modifies the need for nutritive antioxidants, we aimed (1) to investigate the changes of endogenous and exogenous antioxidants in response to an Ironman triathlon; (2) to particularise the relevance of antioxidant responses to the indices of oxidatively damaged blood lipids, blood cell compounds and lymphocyte DNA and (3) to examine whether potential time-points of increased susceptibility to oxidative damage are associated with alterations in the antioxidant status. Blood that was collected from forty-two well-trained male athletes 2 d pre-race, immediately post-race, and 1, 5 and 19 d later was sampled. The key findings of the present study are as follows: (1) Immediately post-race, vitamin C, α-tocopherol, and levels of the Trolox equivalent antioxidant capacity, the ferric reducing ability of plasma and the oxygen radical absorbance capacity (ORAC) assays increased significantly. Exercise-induced changes in the plasma antioxidant capacity were associated with changes in uric acid, bilirubin and vitamin C. (2) Significant inverse correlations between ORAC levels and indices of oxidatively damaged DNA immediately and 1 d post-race suggest a protective role of the acute antioxidant responses in DNA stability. (3) Significant decreases in carotenoids and γ-tocopherol 1 d post-race indicate that the antioxidant intake during the first 24 h of recovery following an acute ultra-endurance exercise requires specific attention. Furthermore, the present study illustrates the importance of a diversified and well-balanced diet to maintain a physiological antioxidant status in ultra-endurance athletes in reference to recommendations.
Spanish Morphological Generation with Wide-Coverage Lexicons and Decision Trees

This work was partly funded by the ABLETO-INCLUDE project (European Commission CIP Grant No. 621055), the TUNER project (TIN2015-65308-C5-5-R, MINECO/FEDER, UE), and the Spanish MINECO Ministry (MDM-2015-0502).
Membrane Topology of the C-terminal Half of the Neuronal, Glial, and Bacterial Glutamate Transporter Family*

Secondary glutamate transporters in neuronal and glial cells in the mammalian central nervous system remove the excitatory neurotransmitter glutamate from the synaptic cleft and prevent the extracellular glutamate concentration to rise above neurotoxic levels. Secondary structure prediction algorithms predict 6 transmembrane helices in the first half of the transporters but fail in the C-terminal half where no clear helix-loop-helix motif is resolved in the hydropathy profile of the primary sequences. A number of previous studies have emphasized the importance of the C-terminal half of the molecules for the function. Here we determine the membrane topology of the C-terminal half of the glutamate transporters by applying the phoA gene fusion technique to the homologous bacterial glutamate transporter of Bacillus stearothermophilus. High sequence conservation and very similar hydropathy profiles in the C-terminal half warrant a similar folding as in the glutamate transporters of the mammalian central nervous system. The C-terminal half contains four putative transmembrane helices. The strong hydrophobic moment and substitution moment of the most C-terminal helix X that point to opposite faces of the helix suggest that the helix faces the lipid environment with its least conserved, hydrophobic face and the interior of the protein with its well conserved, hydrophilic face. Residues that were shown before to be critical for function cluster in helix X and VII.
VIDEO STABILIZATION WITH L 1L 2 OPTIMIZATION

Digital videos often suffer from undesirable camera jitters because of unstable camera motions. In this paper we present a novel video stabilization algorithm by mixed L1-L2 optimization, aiming at removing unwanted camera movements as well as keeping the original video information to the greatest extent. In the proposed algorithm, we compute smoothed camera paths that are composed of constant, linear and parabolic segments by L1 constraints, meanwhile using the L2 norm of the difference between smoothed and original camera paths to retain the video information. Different from other existing methods, there is only one parameter to control effects of two terms, which is both flexible and easy to meet different requirements in practice. We further design an efficient moving window scheme to support online processing or unlimited length video. Experimental results demonstrate the good performance of our proposed algorithm.
Tc-99m HMPAO/SPECT Determination of Regional Cerebral Blood Flow Changes in Schizophrenics

Hypofrontality (hypometabolism and hypoperfusion) has been demonstrated in patients with schizophrenia by the use of several imaging methods, such as positron emission tomography (PET). In this study, Tc-99m hexamethylpropylene amineoxime (HMPAO) and SPECT were used to investigate regional blood flow changes in 20 schizophrenic patients (12 males and 8 females, aged 15—50 years) experiencing acoustic hallucinations. Tc-99m HMPAO (15-20 mCi) was injected intravenously, eight regions/hemisphere were drawn on transaxial sections, and tracer redistribution was calculated for each region. Frontal/occipital ratios (0.917 ± 0.045) and frontal/wholeslice ratios (0.984 ± 0.036) were determined for each patient. The patients had significantly lower frontal/occipital ratios (P < 0.0005) and lower frontal/wholeslice ratios (P < 0.01) in comparison to a normal group (11 cases).
Hemorrhage Prediction Models in Surgical Intensive Care: Bedside Monitoring Data Adds Information to Lab Values

Hemorrhage is a frequent complication in surgery patients; its identification and management have received increasing attention as a target for quality improvement in patient care in the Intensive Care Unit (ICU). The purposes of this work were 1) to find an early detection model for hemorrhage by exploring the range of data mining methods that are currently available, and 2) to compare prediction models utilizing continuously measured physiological data from bedside monitors to those using commonly obtained laboratory tests. We studied 3766 patients admitted to the University of Virginia Health System Surgical Trauma Burn ICU. Hemorrhage was defined as three or more units of red blood cells transfused within 24 h without red blood cell transfusion in the preceding 24 h. 222 patients (5.9%) experienced a hemorrhage, and multivariate models based on vital signs and their trends showed good results (AUC = 76.1%). The hematocrit, not surprisingly, had excellent performance (AUC = 87.7%). Models that included both continuous monitoring and laboratory tests had the best performance (AUC = 92.2%). The results point to a combined strategy of continuous monitoring and intermittent lab tests as a reasonable clinical approach to the early detection of hemorrhage in the surgical ICU.