Miracle-dz/UniFAR-FTUs huggingface dataset:
Developing leaflets to give dental health advice to Aboriginal families with young children.

OBJECTIVES Dental caries (decay) is a serious problem for young Aboriginal children, causing pain and stress. Treatment often involves extraction of teeth under a general anaesthetic. However, dental caries can be prevented by reducing the frequency of sugar consumption and brushing teeth twice a day with fluoride toothpaste. Such straightforward advice could be given to families by Aboriginal Health Workers who are trusted by their communities and have an existing advisory role. This paper reports on the development of dental health advice leaflets for use in Aboriginal communities. METHODS An Aboriginal reference panel was recruited to comment on dental health advice leaflets prepared by an Aboriginal graphic designer. The panel was asked to consider the design, cultural appropriateness and practicality of the leaflets. Comments were collected through email and face-to-face discussions, which were collated and the leaflets altered accordingly. RESULTS The advice from the panel resulted in greater use of pictures. For example large green ticks and red crosses highlighted healthy and unhealthy behaviours, respectively. The tooth brushing leaflet was amended to emphasise the safe storage of toothpaste in order to keep it out of reach of young children. The panel stated that all leaflets should incorporate the Aboriginal flag, and proposed that fridge magnets might be beneficial as all family members would benefit from seeing the messages every day. CONCLUSION The consultation process refined dental advice leaflets to reflect the views of an Aboriginal Reference Panel, in terms of design, cultural competence and practicality.
Cyber-physical-social system in intelligent transportation

A cyber-physical system (CPS) is composed of a physical system and its corresponding cyber systems that are tightly fused at all scales and levels. CPS is helpful to improve the controllability, efficiency and reliability of a physical system, such as vehicle collision avoidance and zero-net energy buildings systems. It has become a hot R&D and practical area from US to EU and other countries. In fact, most of physical systems and their cyber systems are designed, built and used by human beings in the social and natural environments. So, social systems must be of the same importance as their CPSs. The indivisible cyber, physical and social parts constitute the cyber-physical-social system (CPSS), a typical complex system and it's a challengeable problem to control and manage it under traditional theories and methods. An artificial systems, computational experiments and parallel execution (ACP) methodology is introduced based on which data-driven models are applied to social system. Artificial systems, i.e., cyber systems, are applied for the equivalent description of physical-social system (PSS). Computational experiments are applied for control plan validation. And parallel execution finally realizes the stepwise control and management of CPSS. Finally, a CPSS-based intelligent transportation system (ITS) is discussed as a case study, and its architecture, three parts, and application are described in detail.
Effectiveness of Quality of Life Therapy for Depression

A bibliotherapy outcome study was conducted to assess the efficacy of Quality of Life Therapy for depression. Sixteen clinically depressed community volunteers who showed an aptitude for and interest in bibliotherapy and were not suffering from other disorders met weekly to discuss a manual on Quality of Life Therapy. All subjects who completed treatment were reclassified as nondepressed and showed significant increases in quality of life and self-efficacy at the end of treatment. All but one subject maintained these improvements at a follow-up assessment. The potential for increasing the improvement rate of a treatment by matching patients to treatment modalities that fit their aptitudes, skills, and interests is discussed.
Antiestrogen binding in antiestrogen growth-resistant estrogen-responsive clonal variants of MCF-7 human breast cancer cells.

Although antiestrogen therapy is effective in the treatment of hormone-responsive breast tumors, approximately 40% of the patients with estrogen receptor-positive tumors fail to respond to antiestrogens. To better understand the mechanisms by which antiestrogens inhibit the growth of hormone-dependent breast cancers, we have investigated the physicochemical properties and binding characteristics of the estrogen receptors with estradiol and antiestrogens and the occurrence of estrogen-noncompetible antiestrogen binding sites in two estrogen-sensitive but tamoxifen-growth-resistant estrogen receptor-positive MCF-7 cell variant clones, R3-98 and R27. In the variant cells, estradiol (10(-8) M) significantly stimulates cell proliferation as in the parent MCF-7 cells, but the antiestrogen tamoxifen (10(-6) M) has no significant effect on growth of the variant cells, whereas antiestrogen strongly inhibits proliferation of the parent MCF-7 cells. All three cell types contain high concentrations of estrogen receptor (150 to 250 fmol/mg protein), and competition binding analysis shows that the relative binding affinity of a series of compounds for estrogen receptor is similar among the three cell types with the affinity of trans-hydroxytamoxifen greater than estradiol greater than alpha-[4-pyrrolidinoethoxy]phenyl-4-hydroxy-alpha'-nitrostilben e greater than tamoxifen. Salt-extracted nuclear receptor complexes prepared from the three cell types showed similar sedimentation behavior on 0.4 M KCl-containing sucrose gradients with [3H]estradiol-labeled receptor complexes sedimenting at 4.2S, whereas receptors complexed with either of the antiestrogens trans-[3H]-hydroxytamoxifen or [3H]alpha-[4-pyrrolidinoethoxy]phenyl-4-hydroxy-alpha'-nitrosti lbene sediment at 5.5S. In all 3 cell types, the nuclear receptor forms react with an estrogen receptor monoclonal antibody, D547Sp gamma, to form complexes which sediment at 8.5S. The nuclear estrogen receptors from the parental MCF-7 and the two variant cells, when covalently labeled with [3H]-tamoxifen aziridine in intact cells and then salt extracted have identical molecular weights of approximately 62,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The covalently labeled nuclear and cytosol receptors in these 3 cell lines also show identical migration in 8 M urea polyacrylamide isoelectric focusing gels consistent with a predominant receptor species of isoelectric point approximately 5.7. (ABSTRACT TRUNCATED AT 400 WORDS)
Open Palm Menu: A Virtual Menu Placed in Front of the Palm

Virtual and mixed realities make it possible to view and interact with virtual objects in a three-dimensional space. However, the location to display menus in three-dimensional space and the means of manipulating them are often problems. Existing studies developed methods of displaying a menu in the air or on the body. In this paper, a menu system is proposed that appears in front of the user's palm (of the non-dominant hand) when he/she opens that hand. The user employs the other hand (dominant hand) to then interact with the menu. Using the space around the body as opposed to projections onto actual limbs makes it possible to display more items in more varied layouts. Additionally, the user can control rendering of the menu by opening or closing the hand. Being adjacent to the palm of the open hand also enables the user to adjust the hand position to operate the menu more comfortably or to move the menu to an area where it is easier to view. In this study, we conducted an experiment to develop the menu design guidelines to ensure smooth menu operation, designed an optimal menu, and tested that menu by determining the ease of use in interacting with our modeling application.
DNA barcoding and minibarcoding as a powerful tool for feather mite studies

Feather mites (Astigmata: Analgoidea and Pterolichoidea) are among the most abundant and commonly occurring bird ectosymbionts. Basic questions on the ecology and evolution of feather mites remain unanswered because feather mite species identification is often only possible for adult males, and it is laborious even for specialized taxonomists, thus precluding large‐scale identifications. Here, we tested DNA barcoding as a useful molecular tool to identify feather mites from passerine birds. Three hundred and sixty‐one specimens of 72 species of feather mites from 68 species of European passerine birds from Russia and Spain were barcoded. The accuracy of barcoding and minibarcoding was tested. Moreover, threshold choice (a controversial issue in barcoding studies) was also explored in a new way, by calculating through simulations the effect of sampling effort (in species number and species composition) on threshold calculations. We found one 200‐bp minibarcode region that showed the same accuracy as the full‐length barcode (602 bp) and was surrounded by conserved regions potentially useful for group‐specific degenerate primers. Species identification accuracy was perfect (100%) but decreased when singletons or species of the Proctophyllodes pinnatus group were included. In fact, barcoding confirmed previous taxonomic issues within the P. pinnatus group. Following an integrative taxonomy approach, we compared our barcode study with previous taxonomic knowledge on feather mites, discovering three new putative cryptic species and validating three previous morphologically different (but still undescribed) new species.
Mobile user verification/identification using statistical mobility profile

Recent studies show that ubiquitous smartphone data, e.g., the universal cell tower IDs, WiFi access points, etc., can be used to effectively recover individuals' mobility. However, recording and releasing the data containing such information without anonymization can hurt individuals' location privacy. Therefore, many anonymization methods have been used to sanitize these datasets before they are shared to the research community. In this paper, we demonstrate the idea of statistical mobile user profiling and identification based on anonymized datasets. Our insight is that, the mobility patterns inferred from different individuals' data are identifiable by using the statistical profiles constructed from the patterns. Experimental results show that, the proposed method achieves a promising identification accuracy of 96% on average based on randomly chosen two users' data, which makes our framework feasible for the application of inferring the fraud usage of the smartphones. Also, extensive experiments are conducted on the more challenging cases, showing a 59.5% identification accuracy for a total of 50 users based on 636 weekly data segments and a 56.1% accuracy for a total of 63 users based on 786 weekly data segments for two separate datasets. As the first work of such kind, our result suggests good possibility of developing location-based services or applications on the ubiquitous location anonymized datasets.
Effect of aging on brain metabolism in antiretroviral-naive HIV patients

Objective: Normal aging as well as HIV infection may lead to inflammatory changes and injury to the brain; however, it is unclear if and how these processes interact. The goal of this pilot study was to evaluate the interaction between aging and HIV infection in the brain using proton magnetic resonance spectroscopy (1H-MRS). Design: Analyses of covariance (ANCOVA) were performed to determine the effects of HIV and age, and their interaction, on MRS variables. Methods: Forty-six HIV patients naive to antiretroviral medications and 58 seronegative control subjects were examined using localized 1H-MRS in the frontal gray matter, frontal white matter and basal ganglia, and metabolite concentrations were determined. Results: Compared with seronegative controls, HIV-positive subjects showed additional and marked increases in the concentration of glial markers, choline-containing compounds (seronegative controls +2%/decade; HIV-positive subjects +10%/decade) and myoinositol (seronegative controls +3%/decade; HIV-positive subjects +12%/decade), with aging in the frontal white matter. In the basal ganglia, N-acetyl compounds and total creatine decreased with age only in HIV patients (N-acetyl compounds −3.7%/decade; creatine −4%/decade). ANCOVA showed significant interaction effects between HIV and aging on the metabolites in the basal ganglia (N-acetyl peak P = 0.03; creatine P = 0.04) and in the frontal white matter (interaction: choline-containing compounds P = 0.002; myoinositol P = 0.007). Conclusion: In the basal ganglia, HIV infection appeared to induce neuronal damage or loss beyond that observed in normal aging. In the frontal white matter, HIV infection seemed to exacerbate glial activation beyond that observed in normal aging.
What is known about tobacco industry efforts to influence tobacco tax? A systematic review of empirical studies

Objective To systematically review studies of tobacco industry efforts to influence tobacco tax policies. Methods Searches were conducted between 1 October 2009 and 31 March 2010 in 14 databases/websites, in relevant bibliographies and via experts. Studies were included if they focused on industry efforts to influence tobacco tax policies, drew on empirical evidence, were in English and concerned the period 1985–2010. In total, 36 studies met these criteria. Two reviewers undertook data extraction and critical appraisal. A random selection of 15 studies (42%) was subject to second review. Evidence was assessed thematically to identify distinct tobacco industry aims, arguments and tactics. Results A total of 34 studies examined industry efforts to influence tax levels. They suggest the tobacco industry works hard to prevent significant increases and particularly dislikes taxes ‘earmarked’ for tobacco control. Key arguments to counter increases are that tobacco taxes are socially regressive, unfair and lead to increased levels of illicit trade and negative economic impacts. For earmarked taxes, the industry also frequently tries to raise concerns about revenue allocation. Assessing industry arguments against established evidence demonstrates most are unsupported. Key industry tactics include: establishing ‘front groups’, securing credible allies, direct lobbying and publicity campaigns. Only seven studies examined efforts to influence tax structures. They suggest company preferences vary and tactics centre on direct lobbying. Conclusions The tobacco industry has historically tried to keep tobacco taxes low using consistent tactics and misleading arguments. Further research is required to explore efforts to influence tax structures, excise policies beyond the USA and recent policies.
Synchrony of high frequency oscillations in the human epileptic brain

We have applied wavelet phase coherence (WPC) to human iEEG data to characterize the spatial and temporal interactions of high frequency oscillations (HFOs; >80 Hz). Quantitative analyses were performed on iEEG segments from four patients with extratemporal lobe epilepsy. Interelectrode synchrony was measured using WPC before, during and after seizure activity. The WPC profiles of HFOs were able to elucidate the seizure from non-seizure state in all four patients and for all seizures studied (n=10). HFO synchrony was consistently transient and of weak to moderate strength during non-seizure activity, while weak to very strong coherence, of prolonged duration, was observed during seizures. Several studies have suggested that HFOs may have a significant role in the process of epileptogenesis and seizure genesis. As epileptic seizures result from disturbances in the regular electrical activity present in given areas of the brain, studying the interactions between fast brain waves, recorded simultaneously and from many different brain regions, may provide more information of which brain areas are interacting during ictal and interictal activity.
N-Acylhomoserine Lactones Antagonize Virulence Gene Expression and Quorum Sensing in Staphylococcus aureus

ABSTRACT Many gram-negative bacteria employ N-acylhomoserine lactone (AHL)-mediated quorum sensing to control virulence. To determine whether gram-positive bacteria such as Staphylococcus aureus respond to AHLs, we used a growth-dependent lux reporter fusion. Exposure of S. aureus to different AHLs revealed that 3-oxo-substituted AHLs with C10 to C14 acyl chains inhibited light output and growth in a concentration-dependent manner, while short-chain AHLs had no effect. N-(3-Oxododecanoyl)-l-homoserine lactone (3-oxo-C12-HSL) inhibited the production of exotoxins and cell wall fibronectin-binding proteins but enhanced protein A expression. Since these processes are reciprocally regulated via the S. aureus agr quorum-sensing system, which in turn, is regulated via sar, we examined the effect of AHLs on sarA and agr. At sub-growth-inhibitory concentrations of 3-oxo-C12-HSL, both sarA expression and agr expression were inhibited, indicating that the action of 3-oxo-C12-HSL is mediated at least in part through antagonism of quorum sensing in S. aureus. Spent culture supernatants from Pseudomonas aeruginosa, which produces both 3-oxo-C12-HSL and N-butanoyl-homoserine lactone (C4-HSL), also inhibited agr expression, although C4-HSL itself was inactive in this assay. Since quorum sensing in S. aureus depends on the activities of membrane-associated proteins, such as AgrB, AgrC, and AgrD, we investigated whether AHLs perturbed S. aureus membrane functionality by determining their influence on the membrane dipole potential. From the binding curves obtained, a dissociation constant of 7 μM was obtained for 3-oxo-C12-HSL, indicating the presence of a specific saturable receptor, whereas no binding was observed for C4-HSL. These data demonstrate that long-chain 3-oxo-substituted AHLs, such as 3-oxo-C12-HSL, are capable of interacting with the S. aureus cytoplasmic membrane in a saturable, specific manner and at sub-growth-inhibitory concentrations, down-regulating exotoxin production and both sarA and agr expression.
Dosimetry for animals and plants: contending with biota diversity

Diversity of living organisms and their environmental radiation exposure conditions represents a special challenge for non-human dosimetry. In order to contend with such diversity, the International Commission on Radiological Protection (ICRP) has: (a) set up points of reference by providing dose conversion coefficients (DCCs) for reference entities known as ‘Reference Animals and Plants’ (RAPs); and (b) used dosimetric models that pragmatically assume simple body shapes with uniform composition and density, homogeneous internal contamination, a limited set of idealised external radiation sources, and truncation of the radioactive decay chains. This pragmatic methodology has been further developed and extended systematically. Significant methodological changes include: a new extended approach for assessing doses of external exposure for terrestrial animals, transition to the contemporary ICRP radionuclide database, assessment-specific consideration of the contribution of radioactive progeny to dose coefficients of parent nuclides, and the use of generalised allometric relationships in the estimation of biokinetic or metabolic parameters. The new methodological developments resulted in a revision of the DCCs for RAPs. Tables of the dose coefficients have now been complemented by a web-based software tool, which can be used to calculate a user-specific DCC for an organism of arbitrary mass and shape, located at user-defined height above the ground, and for an arbitrary radionuclide and its radioactive progeny.
Nanocarrier-assisted sub-cellular targeting to the site of mitochondria improves the pro-apoptotic activity of paclitaxel

Many drug molecules exert their biological action on intracellular molecular targets present on or inside various cellular organelles. Consequently, it has become more evident that the efficiency and efficacy of drug action is dependent largely on how well an unaided drug molecule is able to reach its intracellular target. We hypothesized that the biological action of such drug molecules might be improved by specific delivery to the appropriate sub-cellular site by a pharmaceutical carrier designed for the purpose. To test our hypothesis, we used paclitaxel, a molecule that has recently been shown to have pro-apoptotic biological targets on the mitochondria but has a quantitative structure–activity relationship-predicted cytosolic accumulation and no affinity for mitochondria. Using a mitochondria-specific nanocarrier system (DQAsomes) prepared from the amphiphilic quinolinium derivative dequalinium chloride to deliver paclitaxel to mitochondria in cells, we report that it is possible to improve the pro-apoptotic action of paclitaxel.
Effect of different angiotensin-converting-enzyme inhibitors on mortality among elderly patients with congestive heart failure

Background: Existing clinical trial data do not address whether all angiotensin-converting-enzyme (ACE) inhibitors are similarly beneficial in improving survival and reducing the rate of readmission among patients with congestive heart failure. We sought to answer this question using administrative databases from Canada's 3 most populous provinces. Methods: Using linked hospital discharge and prescription claims databases in Quebec, Ontario and British Columbia, we identified all patients 65 years or older who were admitted to hospital because of congestive heart failure between Jan. 1, 1998, and Mar. 31, 2002, and who had not been admitted for the same reason in the 3 years preceding the study period. We analyzed the association between the type of ACE inhibitor prescribed within 30 days after discharge and subsequent mortality using Cox proportional hazards models. We then adjusted for demographic, clinical, physician and hospital-related variables, with additional time-dependent variables representing current drug use and dosage. We chose ramipril as the reference category for comparison with the other ACE inhibitors because it has increasingly been prescribed to patients with congestive heart failure. Results: A total of 43 316 patients with congestive heart failure filled prescriptions for ACE inhibitors within 30 days after discharge from hospital. Demographic, clinical and prescription-related characteristics were similar among users of each type of ACE inhibitor. In the time-dependent model, the mortality associated with 5 ACE inhibitors was similar to that with ramipril: adjusted hazard ratios (and 95% confidence intervals [CIs]) were 0.95 (0.89–1.02) for lisinopril, 0.92 (0.85–1.00) for fosinopril, 0.99 (0.88–1.11) for quinapril, 0.90 (0.77–1.06) for perindopril and 1.00 (0.80–1.24) for cilazapril. However, use of enalapril or captopril was associated with higher mortality compared with ramipril: adjusted hazard ratios (and 95% CIs) were 1.10 (1.04–1.16) for enalapril and 1.13 (1.01–1.26) for captopril. Interpretation: When prescribing ACE inhibitors to patients, physicians should consider a possible 10%–15% increase in mortality with captopril and enalapril compared with ramipril among patients with congestive heart failure.