Miracle-dz/UniFAR-FTUs huggingface dataset:
Nomenclature of platelet‐specific antigens

To the Editor: Improvement in the methodology of detection of platelet antigens has led to the discovery of a number of new plateletspecific alloantigen systems. However, many of the new systems as well as the older ones are known under different names. To avoid confusion, the Working Party on Platelet Serology decided to formulate a new nomenclature for such antigen systems. The rules for this nomenclature are as follows: 1. Platelet-specific antigen systems will be called HPA for human platelet antigen(s); 2. The different antigen systems will be numbered chronologically in order of the date of publication; 3. The allelic antigens will be designated alphabetically in order of their frequency (from high to low) in the population; and 4. The inclusion of new HPA systems will require the approval of the Working Party. Table 1 shows the HPA systems recognized to date. The PIE system has not been included because anti-PIE' was probably an isoantibody from the blood of a patient with Bernard Soulier syndrome, and anti-PIE2 is no longer available. ' The working Party on Platelet Serology organized by the Expert Panel on Serology of the International Society of Blood Transfusion (ISBT) and the International Committee for Standardization in Haematology (ICSH) included the members: A. von dem Borne (President), Amsterdam, The Netherlands; F. Dtcary (Secretary), Montreal, Quebec, Canada; J. Kelton, Hamilton, Ontario, Canada; N. Buskard and P. Ballem, Vancouver, BC, Canada; C. van Dalen, Amsterdam; A. Brand, J. van Rood, and F. Claas, Leiden, the Netherlands; W. Ouwehand, Cambridge, UK; R. Doughty, Newcastle-upon-Tyne, UK; A. Waters, P. Metcalfe, and M. Contreras, London, UK; J. McFarland, R. Aster, and T. Kunicki, Milwaukee, WI; S . Slichter and G. Teramura, Seattle, WA; D. Christie and J. McCullough, Minneapolis, MN; P. Lalezari, New York, NY; L. Petz, Los Angeles, CA; W. Rosse, Durham, NC; C. Kaplan, Pans, France; R. Kekomaki, Helsinki, Finland; C. MeullerEckhardt, Giessen, FRG; R. Nordhagen, Oslo, Norway; A. Ribera, Barcelona, Spain; E. Taaning, Hvidore, Denmark; I. Cunningham, Melbourne, Australia; and R. Minchinton, Brisbane, Australia. This proposal has been approved by the Japanese Workshop for Platelet Serology. Members of the Japanese Workshop on Platelet Serology were T. Tuji (President), Y. Shibata (Chairman), and H. Saji (Secretary-General). ALBERT E.G. VON DEM BORNE, MD Central Laboralory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Nefherlarids FRANCINE D ~ C A R Y , MD Canadian Red Cross Bood Transfusion Service Montreal, Quebec, Canada
Risk factors for type 2 diabetes mellitus: An exposure-wide umbrella review of meta-analyses

Background Type 2 diabetes mellitus (T2DM) is a global epidemic associated with increased health expenditure, and low quality of life. Many non-genetic risk factors have been suggested, but their overall epidemiological credibility has not been assessed. Methods We searched PubMed to capture all meta-analyses and Mendelian randomization studies for risk factors of T2DM. For each association, we estimated the summary effect size, its 95% confidence and prediction interval, and the I2 metric. We examined the presence of small-study effects and excess significance bias. We assessed the epidemiological credibility through a set of predefined criteria. Results We captured 86 eligible papers (142 associations) covering a wide range of biomarkers, medical conditions, and dietary, lifestyle, environmental and psychosocial factors. Adiposity, low hip circumference, serum biomarkers (increased level of alanine aminotransferase, gamma-glutamyl transferase, uric acid and C-reactive protein, and decreased level of adiponectin and vitamin D), an unhealthy dietary pattern (increased consumption of processed meat and sugar-sweetened beverages, decreased intake of whole grains, coffee and heme iron, and low adherence to a healthy dietary pattern), low level of education and conscientiousness, decreased physical activity, high sedentary time and duration of television watching, low alcohol drinking, smoking, air pollution, and some medical conditions (high systolic blood pressure, late menarche age, gestational diabetes, metabolic syndrome, preterm birth) presented robust evidence for increased risk of T2DM. Conclusions A healthy lifestyle pattern could lead to decreased risk for T2DM. Future randomized clinical trials should focus on identifying efficient strategies to modify harmful daily habits and predisposing dietary patterns.
A Novel Trans Conformation of Ligand-Free Calmodulin

Calmodulin (CaM) is a highly conserved eukaryotic protein that binds specifically to more than 100 target proteins in response to calcium (Ca2+) signal. CaM adopts a considerable degree of structural plasticity to accomplish this physiological role; however, the nature and extent of this plasticity remain to be fully understood. Here, we report the crystal structure of a novel trans conformation of ligand-free CaM where the relative disposition of two lobes of CaM is different, a conformation to-date not reported. While no major structural changes were observed in the independent N- and C-lobes as compared with previously reported structures of Ca2+/CaM, the central helix was tilted by ∼90° at Arg75. This is the first crystal structure of CaM to show a drastic conformational change in the central helix, and reveals one of several possible conformations of CaM to engage with its binding partner.
An investigation of the use of the Kinect system as a measure of dynamic balance and forward reach in the elderly

Objective: To investigate the reliability and correlations of Kinect-derived valuables of forward reach distance and velocity with the traditional functional reach distance, scores on posturography, and other measures of physical fitness. Design: Observational study. Setting: Community hospital. Subjects: Individuals >65 years who attended a geriatric health examination were enrolled. Main measures: The Kinect system was used to record the reach distance and velocity of the forward reach test. Center of pressure displacement was measured by posturography. Physical fitness performance was assessed using the 2-Minute Step Test, the 30-Second Chair Stand Test, the Sit-and-Reach Test, grip strength, and walking speed. Results: A total of 442 individuals were enrolled (mean age: 73.3 ± 5.2 years). Forward reach tracking using the Kinect system showed good repeatability and correlated with traditional functional reach (r = 0.719, P < 0.001); the reaching velocity correlated with scores on posturography (r = −0.257, P = 0.047). Reach distances were significantly decreased in the older group (≥75 years) than in the younger group (<75 years) (P < 0.001). Conclusion: The Kinect system provides a simple, reliable, and age-sensitive assessment of balance in older adults. The valuables correlate with the traditional functional reach, scores on posturography, and physical fitness performance. It provides alternative representation of both static and dynamic balance function.
Analysis of D2D‐pairing scheme based on Voronoi diagram in cellular networks

This paper presents a device‐to‐device (D2D)–pairing scheme based on Voronoi diagram, which establishes D2D links by the relation of transmission powers and link distances. Using the principle of stochastic geometry and channel inversion, the coverage probability is analyzed, we observe that the co‐channel interference caused by cellular networks is stable in theory, and the influence of link distance parameter is highlighted as well. According to the second‐order moment of link distance, the new metric space of D2D terminals set is constructed, in which Voronoi diagram is degenerated into Delaunay triangles set to represent the potential D2D links. In addition, on the basis of the mini‐max length criterion of Delaunay triangle, the D2D communication range–limiting algorithm is designed. Finally, the more intuitive results compared with conventional D2D communications are displayed by simulations. Owing to restraining the D2D communication range, lower co‐channel interference can be obtained. Specially, the D2D‐pairing scheme is approximate local optimal under the D2D communication range–limiting algorithm, and it can provide more fairness than greedy algorithm.
SELECTIVE OXIDATION OF HIPCO SINGLE-WALL CARBON NANOTUBES

The structure of HipCO single-wall carbon nanotubes was controlled by Raman scattering during the material heating in three ways: in a laser beam ("in situ"), in an optical oven ("in situ") and after a preliminary heating in the oven followed by Raman measurements. All experiments have demonstrated a diameter- selective oxidative etching of the smallest nanotubes (8 - 10 A) at temperatures 400 - 450 o C.
A preliminary oncologic outcome and postoperative complications in patients undergoing robot-assisted radical cystectomy: Initial experience

Purpose Robot-assisted radical cystectomy (RARC) was originally intended to replace open radical cystectomy (ORC) as a minimally invasive surgery for patients with invasive bladder cancer. The purpose of this study was to evaluate the advantages of robotic surgery, comparing perioperative and oncologic outcomes between RARC and ORC. Materials and Methods Between June 2012 and August 2016, 49 bladder cancer patients were given a radical cystectomy, 21 robotically and 28 by open procedure. We compared the clinical variables between the RARC and ORC groups. Results In the RARC group, the median estimated blood loss (EBL) during cystectomy, total EBL, operative time during cystectomy, and total operative time were 0 mL, 457.5 mL, 199 minutes, and 561 minutes, respectively. EBL during cystectomy (p<0.001), total EBL (p<0.001), and operative time during cystectomy (p=0.003) in the RARC group were significantly lower compared with the ORC group. Time to resumption of a regular diet (p<0.001) and length of stay (p=0.017) were also significantly shorter compared with the ORC group. However, total operative time in the RARC group (median, 561 minutes) was significantly longer compared with the ORC group (median, 492.5 minutes; p=0.015). Conclusions This Japanese study presented evidence that RARC yields benefits in terms of BL and time to regular diet, while consuming greater total operative time. RARC may be a minimally invasive surgical alternative to ORC with less EBL and shorter length of stay.
Codon misreading tRNAs promote tumor growth in mice

ABSTRACT Deregulation of tRNAs, aminoacyl-tRNA synthetases and tRNA modifying enzymes are common in cancer, raising the hypothesis that protein synthesis efficiency and accuracy (mistranslation) are compromised in tumors. We show here that human colon tumors and xenograft tumors produced in mice by two epithelial cancer cell lines mistranslate 2- to 4-fold more frequently than normal tissue. To clarify if protein mistranslation plays a role in tumor biology, we expressed mutant Ser-tRNAs that misincorporate Ser-at-Ala (frequent error) and Ser-at-Leu (infrequent error) in NIH3T3 cells and investigated how they responded to the proteome instability generated by the amino acid misincorporations. There was high tolerance to both misreading tRNAs, but the Ser-to-Ala misreading tRNA was a more potent inducer of cell transformation, stimulated angiogenesis and produced faster growing tumors in mice than the Ser-to-Leu misincorporating tRNA. Upregulation of the Akt pathway and the UPR were also observed. Most surprisingly, the relative expression of both misreading tRNAs increased during tumor growth, suggesting that protein mistranslation is advantageous in cancer contexts. These data highlight new features of protein synthesis deregulation in tumor biology.
Calculating the quality of public high-throughput sequencing data to obtain a suitable subset for reanalysis from the Sequence Read Archive

Abstract It is important for public data repositories to promote the reuse of archived data. In the growing field of omics science, however, the increasing number of submissions of high-throughput sequencing (HTSeq) data to public repositories prevents users from choosing a suitable data set from among the large number of search results. Repository users need to be able to set a threshold to reduce the number of results to obtain a suitable subset of high-quality data for reanalysis. We calculated the quality of sequencing data archived in a public data repository, the Sequence Read Archive (SRA), by using the quality control software FastQC. We obtained quality values for 1 171 313 experiments, which can be used to evaluate the suitability of data for reuse. We also visualized the data distribution in SRA by integrating the quality information and metadata of experiments and samples. We provide quality information of all of the archived sequencing data, which enable users to obtain sufficient quality sequencing data for reanalyses. The calculated quality data are available to the public in various formats. Our data also provide an example of enhancing the reuse of public data by adding metadata to published research data by a third party.
Diabetes Reduces Severity of Aortic Aneurysms Depending on the Presence of Cell Division Autoantigen 1 (CDA1)

Diabetes is a negative risk factor for aortic aneurysm, but the underlying explanation for this phenomenon is unknown. We have previously demonstrated that cell division autoantigen 1 (CDA1), which enhances transforming growth factor-β signaling, is upregulated in diabetes. We hypothesized that CDA1 plays a key role in conferring the protective effect of diabetes against aortic aneurysms. Male wild-type, CDA1 knockout (KO), apolipoprotein E (ApoE) KO, and CDA1/ApoE double-KO (dKO) mice were rendered diabetic. Whereas aneurysms were not observed in diabetic ApoE KO and wild-type mice, 40% of diabetic dKO mice developed aortic aneurysms. These aneurysms were associated with attenuated aortic transforming growth factor-β signaling, reduced expression of various collagens, and increased aortic macrophage infiltration and matrix metalloproteinase 12 expression. In the well-characterized model of angiotensin II–induced aneurysm formation, concomitant diabetes reduced fatal aortic rupture and attenuated suprarenal aortic expansion, changes not seen in dKO mice. Furthermore, aortic CDA1 expression was downregulated ∼70% within biopsies from human abdominal aortic aneurysms. The identification that diabetes is associated with upregulation of vascular CDA1 and that CDA1 deletion in diabetic mice promotes aneurysm formation provides evidence that CDA1 plays a role in diabetes to reduce susceptibility to aneurysm formation.
Current views on the potential for development of a HIV vaccine

ABSTRACT Introduction: Despite many recent advances in the HIV prevention landscape, an effective vaccine remains the most promising tool to end the HIV-1 pandemic. Areas covered: This review summarizes past HIV vaccine efficacy trials and current vaccine strategies as well as new approaches about to move into first-in-human trials. Expert opinion: Despite many setbacks in early HIV vaccine efficacy trials, the success of RV144 has provided the glimmer of hope necessary to invigorate the vaccine field, and has led to the development of a large number of vaccine strategies aiming at inducing an array of different immune responses. The follow-up pox-protein trials, developed to replicate and enhance the polyfunctional antibody responses induced by the RV144 regimen, are already reaching efficacy trials, while a large body of work providing a more complete understanding of the development of broadly neutralizing antibodies is now being translated into immunogen design using several different strategies. T-cell based vaccines, fallen out of favor after Ad5-based trials showed increased infection rates in Ad5 seropositive vaccine recipients, are experiencing a comeback based in part on the promising results from non-human primate challenge studies using rhCMV-based immunogens. This diverse array of vaccine candidates may finally allow us to identify a broadly effective HIV vaccine able to contain the epidemic.
Paederus dermatitis

Paederus dermatitis is an irritant dermatitis caused by pederin, a toxin produced by the rove beetle (Paederus). The disease occurs worldwide, but it is often not recognized as a history of contact with the insect is frequently absent. It is commonly seen in the rainy season. Crushing the insect releases pederin, resulting in the characteristic linear lesions with a burning sensation that heal with hyperpigmentation. Treatment comprises immediate washing of the area to eliminate the toxin and topical application of a topical steroid-antibiotic combination. Preventive measures may include reducing the insect population in the surroundings, avoiding contact of insects with the skin, minimizing the lesions after contact. Awareness of the etiology and clinical manifestations makes it easier to suspect this condition even in the absence of a history of exposure to the insect. We present a comprehensive review of the etiopathogenesis, pathology, clinical features, treatment and prevention of Paederus dermatitis and also review the biology of the insect and its behavior.
Network Analysis Highlights Complex Interactions between Pathogen, Host and Commensal Microbiota

Interactions between bacteria and their host represent a full continuum from pathogenicity to mutualism. From an evolutionary perspective, host-bacteria relationships are no longer considered a two-component system but rather a complex network. In this study, we focused on the relationship between brook charr (Salvelinus fontinalis) and bacterial communities developing on skin mucus. We hypothesized that stressful conditions such as those occurring in aquaculture production induce shifts in the bacterial community of healthy fish, thus allowing pathogens to cause infections. The results showed that fish skin mucus microbiota taxonomical structure is highly specific, its diversity being partly influenced by the surrounding water bacterial community. Two types of taxonomic co-variation patterns emerged across 121 contrasted communities’ samples: one encompassing four genera well known for their probiotic properties, the other harboring five genera mostly associated with pathogen species. The homeostasis of fish bacterial community was extensively disturbed by induction of physiological stress in that both: 1) the abundance of probiotic-like bacteria decreased after stress exposure; and 2) pathogenic bacteria increased following stress exposure. This study provides further insights regarding the role of mutualistic bacteria as a primary host protection barrier.
Rapid Identié cation of Chemical Warfare Agents by Artié cial Neural Network Pruning of Temperature-Programmed Microsensor Databases

Rapid identie cation of three chemical warfare (CW) agents and a CW agent simulant has been achieved by analyzing the responses of an array of four microhotplate conductometric sensors with tin oxide and titanium oxide thin sensing e lms. Analyte concentration values in the range of nmol/mol (ppb) to mmol/mol (ppm) were also determined. Calculating the ratios of the response onset and recovery time constants of the different sensor materials at different temperatures, when operated in the e xed temperature sensing mode, clearly identie ed each CW agent. Training artie cial neural network (ANN) models from an 80-component response database (four sensing e lms at 20 operating temperature steps), obtained in the temperature-programmed sensing operating mode, led to successful individual analyte recognition and four separate agent concentration models to provide the concentrations of the target compounds. Recursive elimination of the less relevant inputs and ANN model re-training identie ed the 5 to 12 inputs that are sufe cient to identify and quantify the CW agents. The information obtained through pruning allows one to reduce the microsensor scan time by 40% to 80% and provides insight into the nature of the most critical gas‐ solid interactions for detection.
Proteomic Differences in Feline Fibrosarcomas Grown Using Doxorubicin-Sensitive and -Resistant Cell Lines in the Chick Embryo Model

Proteomic analyses are rapid and powerful tools that are used to increase the understanding of cancer pathogenesis, discover cancer biomarkers and predictive markers, and select and monitor novel targets for cancer therapy. Feline injection-site sarcomas (FISS) are aggressive skin tumours with high recurrence rates, despite treatment with surgery, radiotherapy, and chemotherapy. Doxorubicin is a drug of choice for soft tissue sarcomas, including FISS. However, multidrug resistance is one of the major causes of chemotherapy failure. The main aim of the present study was to identify proteins that differentiate doxorubicin-resistant from doxorubicin-sensitive FISS using two-dimensional gel electrophoresis (2DE), followed by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) analysis. Using the three-dimensional (3D) preclinical in ovo model, which resembles features of spontaneous fibrosarcomas, three significantly (p ≤ 0.05) differentially expressed proteins were identified in tumours grown from doxorubicin-resistant fibrosarcoma cell lines (FFS1 and FFS3) in comparison to the doxorubicin-sensitive one (FFS5): Annexin A5 (ANXA5), Annexin A3 (ANXA3), and meiosis-specific nuclear structural protein 1 (MNS1). Moreover, nine other proteins were significantly differentially expressed in tumours grown from the high doxorubicin-resistant cell line (FFS1) in comparison to sensitive one (FFS5). This study may be the first proteomic fingerprinting of FISS reported, identifying potential candidates for specific predictive biomarkers and research targets for doxorubicin-resistant FISS.
The neurobiological basis of human aggression: A review on genetic and epigenetic mechanisms

Aggression is an evolutionary conserved behavior present in most species including humans. Inadequate aggression can lead to long‐term detrimental personal and societal effects. Here, we differentiate between proactive and reactive forms of aggression and review the genetic determinants of it. Heritability estimates of aggression in general vary between studies due to differing assessment instruments for aggressive behavior (AB) as well as age and gender of study participants. In addition, especially non‐shared environmental factors shape AB. Current hypotheses suggest that environmental effects such as early life stress or chronic psychosocial risk factors (e.g., maltreatment) and variation in genes related to neuroendocrine, dopaminergic as well as serotonergic systems increase the risk to develop AB. In this review, we summarize the current knowledge of the genetics of human aggression based on twin studies, genetic association studies, animal models, and epigenetic analyses with the aim to differentiate between mechanisms associated with proactive or reactive aggression. We hypothesize that from a genetic perspective, the aminergic systems are likely to regulate both reactive and proactive aggression, whereas the endocrine pathways seem to be more involved in regulation of reactive aggression through modulation of impulsivity. Epigenetic studies on aggression have associated non‐genetic risk factors with modifications of the stress response and the immune system. Finally, we point to the urgent need for further genome‐wide analyses and the integration of genetic and epigenetic information to understand individual differences in reactive and proactive AB. © 2015 Wiley Periodicals, Inc.
Biomimetic elastomeric poly(glycerol sebacate)-based scaffolds for adipose tissue engineering

In spite of recent progress in the field of adipose tissue engineering, the optimal adipose tissue scaffold still remains illusive and the tailoring of the structure and properties of tissue scaffolds according to adipose tissue were less explored or even neglected. Thus, synthetic poly(glycerol sebacate) (PGS)-based scaffolds and hydrogels which mimic the properties of adipose tissue were developed in this PhD project for potential application in adipose tissue engineering.