Miracle-dz/UniFAR-FTUs huggingface dataset:
An Inner Pore Residue (Asn406) in the Nav1.5 Channel Controls Slow Inactivation and Enhances Mibefradil Block to T-Type Ca2+ Channel Levels

Mibefradil is a tetralol derivative once marketed to treat hyper-tension. Its primary target is the T-type Ca2+ channel (IC50, ∼0.1-0.2 μM), but it also blocks Na+,K+,Cl-, and other Ca2+ channels at higher concentrations. We have recently reported state-dependent mibefradil block of Na+ channels in which apparent affinity was enhanced when channels were recruited to slow-inactivated conformations. The structural determinants controlling mibefradil block have not been identified, although evidence suggests involvement of regions near or within the inner pore. We tested whether mibefradil interacts with the local anesthetic (LA) binding site, which includes residues in the S6 segments of domains (D) I, III, and IV. Mutagenesis of DIII S6 and DIVS6 did not reveal critical binding determinants. Substitution of Asn406 in DI S6 of cardiac Nav1.5, however, altered affinity in a manner dependent on the identity of the substituting residue. Replacing Asn406 with a phenylalanine or a cysteine increased affinity by 4- and 7-fold, respectively, thus conferring T-type Ca2+ channel-like mibefradil sensitivity to the Na+ channel. A series of other substitutions that varied in size, charge, and hydrophobicity had minimal effects on mibefradil block, but all mutations dramatically altered the magnitude and voltage-dependence of slow inactivation, consistent with data in other isoforms. Channels did not slow-inactivate, however, at the voltages used to assay mibefradil block, supporting the idea that Asn406 lies within or near the mibefradil binding site.
Implementation of cloud based live streaming for surveillance

Rapid technological growth made surveillance as most promising application domain. With great extent of smart city most of the things are controlled by internet. Security is one of the applications that everyone needs to be controlled remotely. This paper presents cloud based surveillance system for live video streaming that can be surveillance from anywhere and anytime. This system provides the live streaming by using cloud; Raspberry Pi 2 module and FFMPEG based USB Camera.
Representative Subgraph Sampling using Markov Chain Monte Carlo Methods

Bioinformatics and the Internet keep generating graph data with thousands of nodes. Most traditional graph algorithms for data analysis are too slow for analysing these large graphs. One way to work around this problem is to sample a smaller ‘representative subgraph’ from the original large graph. Existing representative subgraph sampling algorithms either randomly select sets of nodes or edges, or they explore the vicinity of a randomly drawn node. All these existing approaches do not make use of topological properties of the original graph and provide good samples down to sample sizes of approximately 15% of the number of nodes in the original graph. In this article, we propose novel sampling methods for representative subgraph sampling, based on the Metropolis algorithm and Simulated Annealing. The key idea is to find a subgraph that preserves properties of the original graph that are efficient to compute or to approximate. In our experiments, we improve over the pioneering work of Leskovec and Faloutsos (KDD 2006), by producing representative subgraph samples that are both smaller and of higher quality than those produced by other methods from the literature.
Learning Structural Semantics for the Web

Researchers have long envisioned a Semantic Web, where un-structured Web content is replaced by documents with rich semantic annotations. Unfortunately, this vision has been hampered by the difﬁculty of acquiring semantic metadata for Web pages. This paper introduces a method for automatically "semantifying" structural page elements: using machine learning to train classiﬁers that can be applied in a post-hoc fashion. We focus on one popular class of semantic iden-tiﬁers: those concerned with the structure —or information architecture—of a page. To determine the set of structural semantics to learn and to collect training data for the learning, we gather a large corpus of labeled page elements from a set of online workers. We discuss the results from this collection and demonstrate that our classiﬁers learn structural semantics in a general way.
The paraventricular nucleus of the hypothalamus: Cytoarchitectonic subdivisions and organization of projections to the pituitary, dorsal vagal complex, and spinal cord as demonstrated by retrograde fluorescence double‐labeling methods

Experiments using two retrogradely transported fluorescent dyes (bisbenzimide‐true blue, and Evans blue‐granular blue) were performed in order to determine whether the same or different populations of neurons in the paraventricular nucleus of the hypothalamus (PVH) project to the pituitary gland, dorsal vagal complex, and spinal cord in the rat. The results suggest that cells projecting to the pituitary gland are concentrated in the magnocellular core of the nucleus, while the descending connections arise primarily from the surrounding parvocellular division. The occurrence of neurons double‐labeled with both dyes further indicate that at least 10–15% of the labeled cells in the parvocellular division send divergent axon collaterals to the dorsal vagal complex and to the spinal cord. Cell counts suggest that at least 1,500 cells in the PVH project to the medulla and/or spinal cord. These results, combined with a cytoarchitectonic analysis, show that the PVH consists of eight distinct subdivisions, three magnocellular and five parvocellular. The lateral hypothalamic area and zona incerta also contain a large number of cells projecting to the dorsomedial medulla and spinal cord; approximately 15% of such cells are double‐labeled following injections of separate tracers into these two regions of the same animal.
Synthesis and structure features of the Ca1−2xCe2xMo1−xGexO4 (0 ≤ x≤ 0.2) compounds

The Ca<inf>1−2x</inf>Ce<inf>2x</inf>Mo<inf>1−x</inf>Ge<inf>x</inf>O<inf>4</inf> (0 ≤ × ≤ 0.2) micro/nanosized powders were prepared by aqueous nitrate-tartrate sol-gel synthesis. The obtained samples were characterized by XRD analysis, SEM microscopy, and IR spectroscopy. It was shown, that increase of cerium concentration in the series of compounds does not lead to obvious changes in their crystal structure, but changes of unit cell lattice parameters was fixed. According to SEM analysis, particles of investigation molibdates have diameter 100–500 nm. Luminescence properties of the synthesized powders were studied. The photoluminescence spectra contain wide bands of matrix emission and narrow lines caused by f-f transitions in the RE<sup>3+</sup> ions.
Combining next‐generation sequencing with microarray for transcriptome analysis in rainbow trout gonads

Microarray technology is a powerful tool for studying genome‐wide gene expression. As the genome of many fish has not yet been determined, however, cDNA microarrays can only be designed from limited expressed sequence tag data. In this study, we designed a microarray based on the sequencing data (337,466 reads) obtained by next‐generation sequencing of RNA extracted from rainbow trout (Oncorhynchus mykiss) embryonic genital ridge, testis, and ovary. These data (307,264 reads) were assembled into 28,668 contigs; 3,298 reads could not be assembled and 26,904 reads were unique sequences that did not cluster with other reads. Based on this information, 55,928 microarray probes were designed for a microarray, which was validated by hybridization experiments with RNA extracted from type A spermatogonia (A‐SG) and testicular somatic cells. Expression of known spermatogonial markers was confirmed to be higher in A‐SG than in testicular somatic cells whereas supporting‐cell markers were expressed at higher levels in testicular somatic cells. This microarray analysis revealed that 8,068 transcripts showed at least fourfold higher signal in A‐SG than testicular somatic cells. Fourteen of 17 randomly selected transcripts were expressed at significantly higher‐levels in A‐SG than somatic cells, by quantitative RT‐PCR. In addition, three transcripts analyzed with in situ hybridization showed A‐SG‐specific signals in immature trout testis, with one of them exhibiting a heterogeneous expression pattern in A‐SG. The rainbow trout gonad microarray developed in this study therefore appears to be a useful tool to understand gametogenesis in rainbow trout. Mol. Reprod. Dev. 79: 870–878, 2012. © 2012 Wiley Periodicals, Inc.
Long-term Persistence of Zoster Vaccine Ef fi cacy

Vicki A. Morrison, Gary R. Johnson, Kenneth E. Schmader, Myron J. Levin, Jane H. Zhang, David J. Looney, Robert Betts, Larry Gelb, John C. Guatelli, Ruth Harbecke, Connie Pachucki, Susan Keay, Barbara Menzies, Marie R. Griffin, Carol A. Kauffman, Adriana Marques, John Toney, Kathy Boardman, Shu-Chih Su, Xiaoming Li, Ivan S. F. Chan, Janie Parrino, Paula Annunziato, and Michael N. Oxman; for the Shingles Prevention Study Group Veterans Affairs Medical Center and University of Minnesota, Minneapolis; Cooperative Studies Program Coordinating Center, Veterans Affairs Connecticut Healthcare System, West Haven; Geriatric Research Education and Clinical Centers (GRECC), Durham Veterans Affairs Medical Center and Duke University, North Carolina; University of Colorado Denver, Anschutz Medical Campus, Aurora; Veterans Affairs San Diego Healthcare System and University of California, San Diego; University of Rochester, New York; Veterans Affairs Medical Center, St Louis, Missouri; Hines Veterans Affairs Medical Center, Chicago, Illinois; Veterans Affairs Maryland Health Care System and University of Maryland, Baltimore; Veterans Affairs Medical Center, Seattle, Washington; Vanderbilt University and Mid-South GRECC, Veterans Affairs Tennessee Valley Healthcare System, Nashville; Veterans Affairs Ann Arbor Health Care System and University Of Michigan, Ann Arbor; National Institute of Allergy and Infectious Diseases, Bethesda, Maryland; Veterans Affairs Medical Center, Tampa, Florida; Cooperative Studies Program Central Research Pharmacy Coordinating Center, Albuquerque, New Mexico; and Merck and Co, Whitehouse Station, New Jersey
Alternative Binding Modes Identified for Growth and Differentiation Factor-associated Serum Protein (GASP) Family Antagonism of Myostatin*

Background: GASP-1 and GASP-2 are highly specific antagonists for the TGF-β ligand myostatin, a negative regulator of muscle growth. Results: GASP-1 and GASP-2 form asymmetric and symmetric complexes with myostatin, respectively. Conclusion: Despite the different binding modes, the GASP proteins retain a high specificity for myostatin. Significance: Inhibition of myostatin can be achieved using different binding modes and may facilitate future development of novel anti-myostatin therapeutics. Myostatin, a member of the TGF-β family of ligands, is a strong negative regulator of muscle growth. As such, it is a prime therapeutic target for muscle wasting disorders. Similar to other TGF-β family ligands, myostatin is neutralized by binding one of a number of structurally diverse antagonists. Included are the antagonists GASP-1 and GASP-2, which are unique in that they specifically antagonize myostatin. However, little is known from a structural standpoint describing the interactions of GASP antagonists with myostatin. Here, we present the First low resolution solution structure of myostatin-free and myostatin-bound states of GASP-1 and GASP-2. Our studies have revealed GASP-1, which is 100 times more potent than GASP-2, preferentially binds myostatin in an asymmetrical 1:1 complex, whereas GASP-2 binds in a symmetrical 2:1 complex. Additionally, C-terminal truncations of GASP-1 result in less potent myostatin inhibitors that form a 2:1 complex, suggesting that the C-terminal domains of GASP-1 are the primary mediators for asymmetric complex formation. Overall, this study provides a new perspective on TGF-β antagonism, where closely related antagonists can utilize different ligand-binding strategies.
Metacognitive Awareness Inventory: Tradução e Validação a partir de uma Análise Fatorial Confirmatória

Este estudio tuvo como objetivo traducir y validar estadisticamente el Inventario conciencia metacognitiva (MAI), nuevo instrumento en la literatura en lengua portuguesa. Se recogieron 1.058 respuestas de los estudiantes y profesionales de la ciudad de Administracion de Salvador, Brazil. Traduccion adopto la traduccion de vuelta metodologia y validacion estadistica fue a traves de analisis factorial confirmatorio mediante modelos de ecuaciones estructurales. La “conciencia metacognitiva” constructo relacionado con MAI confirmo que las dimensiones “habilidades de conciencia conocimientos y estrategias metacognitivas” y “Conciencia de estrategias metacognitivas Configuracion” que se presentan las medidas de fiabilidad compuesta y la validez discriminante confirman que las correlaciones entre estos indicadores y sus dimensiones son mas fuertes que las correlaciones entre las dimensiones, es decir, la “conciencia metacognitiva” constructo cumple la validez discriminante , que permite validar el instrumento traducido.
Non Destructive Detection of Zea mays' critical periods

Because of its significance for the improvement of the efficiency in crop production, it is of fundamental importance to identify the plant critical periods (which characterize the sensitivity of the plant to the atmospheric elements). Since in the case of some development stages it is not possible to observe them by a simple sight, it is usual to destroy some sampling plants to get the required information. In this work, the crop growth process of the maize (Zea mays, sp) is analyzed for finding its critical phases by means of non destructive techniques. For this, at first the stem elongation process is analyzed by studying the dynamical behavior of an hypothetic unitary mass particle, concentrated in the stem appendix, traveling through an apparent potential well. And then, a Non Destructive Detection Procedure is proposed, which is based on two concatenated Recursive Constrained Least-Square Algorithm with orthogonal projections. The critical periods are predicted until 3 weeks of anticipation.
Reinforcing model of feeling of energy, empowerment and employees' creativity through ethical leadership

Background and aims: Managers who rely on ethical values, have considerable effects on employees' feeling of energy, empowerment and creativity. Ethical leadership which is a relative new construct, has considerable role in creating healthy work climate. In this regard, this research was administered with the aim of investigating the role of ethical leadership on reinforcing feeling of energy, empowerment and employees' creativity. Methods: Research statistical population were the total of Haier factory employees in autumn of 1388. Among them, 332 persons as the research sample were selected with the use of stratified sampling (from personnel's list of each section). Research instruments which used for assessment of variables including feeling of energy at work with 8 items , creativity at work with 9 items, psychological empowerment with 12 items and ethical leadership with 6 items. Data were analyzed using Pearson's correlation coefficient, structure equation modeling and mediating regression analysis. Results: Results showed that ethical leadership directly reinforces empowerment and energy at work and feeling of energy at work also reinforces empowerment. But in a process analysis, feeling of energy and psychological empowerment after being reinforced through ethical leadership, reinforce employees' creativity at work. Conclusion: The evidences of this research revealed the role of leaders and managers' tendencies to ethical leadership on feeling of energy at work, psychological empowerment and employees' creativity at work. On the basis of the findings, for reinforcing employees' vitality, empowerment and creativity, supervisors and managers' should be complied with ethical leadership principles in their management.
Building model trains and planes: An autoethnographic investigation of a human occupation

ABSTRACT This research utilised an autoethnographic method, termed collective autobiography, to explore the nature and meaning of the amateur hobby of building models from childhood to adulthood. Hobbies and leisure activities are areas of human occupation of increasing interest to a variety of disciplines. Although model making may concern the miniature representation of any subject, this paper focuses on the construction of model aircraft kits, trains and their layouts. As a complex, specific human occupation, modelling is found to contribute to personal well-being and, while the occupation may start in childhood, its associated motivations and required skills develop over a life time. The findings also reveal aspects of the nature of the relationship between the modeller, the process of modelling, and the final product. In addition elements of the gendered nature of modelling, its role within father-son relationships, and the accommodation of modelling within shared domestic spaces are brought to light. The modeller is seen to be a creative individual and a consumer. The specific modelling occupations described are recognised as having their origins within the culture of post-war baby boomer Britain, and the socioeconomic and technological environment of that period.
Improving requirements management in extreme programming with tool support - an improvement attempt that failed

While extreme programming (XP) relies on certain principles, it requires an extensive set of tools to enable an effective execution of its practices. In many companies, putting stories on the board may not be sufficient for managing rapidly changing requirements. The objective of This work is to report the results from a study where a requirement management tool - the Storymanager - was developed to meet the needs of a XP project team. The tool was used in a case project where a mobile application for real markets was produced. The tool was dropped by the team only after two releases. The reasons of the process improvement failure are addressed in This work. The principal results show that the tool was found to be too difficult to use and that it failed to provide as powerful a visual view as the paper-pen board method. The implications of these findings are addressed for both the practitioners and researchers in the field.
Stacking and Analysis of Melamine in Milk Products with Acetonitrile-Salt Stacking Technique in Capillary Electrophoresis

Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 μmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 μmol/L ~ 10.0 μmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step.
Story Schemes for Argumentation about the Facts of a Crime

In the literature on reasoning on the basis of evidence, two traditions exist: one argument-based, and one based on narratives. Recently, we have proposed a hybrid perspective in which argumentation and narratives are combined. This formalized hybrid theory has been tested in a sense-making software prototype for criminal investigators and decision makers. In the present paper, we elaborate on the role of commonsense knowledge. We argue that two kinds of knowledge are essential: argumentation schemes and story schemes. We discuss some of the research issues that need to be addressed.
Semantics-Based Composition of Factory Automation Processes Encapsulated by Web Services

This paper presents an approach to using semantic web services in managing production processes. In particular, the devices in the production systems considered expose web service interfaces through which they can then be controlled, while semantic web service descriptions formulated in web ontology language for services (OWL-S) make it possible to determine the conditions and effects of invoking the web services. The approach involves three web services that cooperate to achieve production goals using the domain web services. In particular, one of the three services maintains a semantic model of the current state of the system, while another uses the model to compose the domain web services so that they jointly achieve the desired goals. The semantic model of the system is automatically updated based on event notifications sent by the domain services.
Synchronization of Identical Oscillators Coupled Through a Symmetric Network With Dynamics: A Constructive Approach With Applications to Parallel Operation of Inverters

We consider the problem of synchronizing a group of N identical oscillators, coupled by a symmetric network that is modelled by a multiple-input/multiple-output dynamical system. We provide results that can be used to establish asymptotic synchronization of a given system and also to construct identical feedback oscillators for which synchronization is guaranteed. These results are based on a new notion of passivity with respect to manifolds defined in the input and output spaces of a dynamical system. The problem under consideration is motivated by the design of control algorithms for the parallel operation of power inverters. Simulation results have shown that synchronization could be achieved in less than two AC cycles, depending on the electrical interconnection network.
Multilingual Restricted Domain QA System with Dialogue Management

The architecture of a multilingual restricted domain question answering system with dialogue management has been proposed. The system maintains language specific domain and linguistic models. The user specifies the query language and a shallow parser for the language analyzes the input text query to obtain keyword and information chunks for identifying the query topic and subsequent generation of the SQL statements. The dialogue manager detects the presence of incomplete information in the input and predicts user intentions to make recommendations based on user model and domain ontology to get user response for completing the query. The dialogue model is a finite state machine in which each dialogue state is defined with the parameters: clarification requests, intended dialogue acts and discourse goals. The system maintains dialogue history for each query topic and for each language containing values of information chunks and the answers for the previous queries in the dialogue. The answer generation process retrieves data from the database and fills slots in the answer templates specific to query topic and language. Null answers are explained and recommendations are made suggesting alternative answers. The system has been developed in the Indian Railways information domain for accepting Telugu and Bengali text inputs and has been evaluated.
Preprocessing the web server logs: an illustrative approach for effective usage mining

Data preprocessing is an important activity for discovering behavioral patterns. The analysis of web logs is an essential task for System Administrators to safeguard adequate bandwidth and to maintain server capacity on their business websites. A web Log file represents user activities occurring over a period of time. Web log files offer valuable insight into the effective usage of the web site. It helps maintain an account of the actual usage in a regular working system as compared to the virtual setting of a usability lab. This research paper focuses on the preprocessing techniques implemented on a specially designed Web Sift (WebIS) tool on an IIS web server and also proposes some efficient heuristics and techniques