Miracle-dz/UniFAR-FTUs huggingface dataset:
An SiC inverter for high speed permanent magnet synchronous machines

This paper present experimental results obtained during the construction of an SiC based inverter for high speed permanent magnet synchronous machines (PMSM). First a comparison between an IGBT and SiC modules used for the same application is presented. The modules static and switching losses are experimentally obtained and compared. The SiC module show significantly lower switching losses. Then the SiC inverter design is described and experimental results are shown. It is found that parasitic inductances in both the gate driver and DC intermediate circuit play a major role and need to be minimized. One procedure using vertically aligned PCB traces is described and measured results are presented. The presented SiC based inverter was successfully tested with the high speed PMSM up to the application full voltage 600V. It was also tested with a high switching frequency up to 500kHz although the target frequency is only 100kHz.
Involvement of Escherichia coli DNA polymerase II in response to oxidative damage and adaptive mutation

DNA polymerase II (Pol II) is regulated as part of the SOS response to DNA damage in Escherichia coli. We examined the participation of Pol II in the response to oxidative damage, adaptive mutation, and recombination. Cells lacking Pol II activity (polB delta 1 mutants) exhibited 5- to 10-fold-greater sensitivity to mode 1 killing by H2O2 compared with isogenic polB+ cells. Survival decreased by about 15-fold when polB mutants containing defective superoxide dismutase genes, sodA and sodB, were compared with polB+ sodA sodB mutants. Resistance to peroxide killing was restored following P1 transduction of polB cells to polB+ or by conjugation of polB cells with an F' plasmid carrying a copy of polB+. The rate at which Lac+ mutations arose in Lac- cells subjected to selection for lactose utilization, a phenomenon known as adaptive mutation, was increased threefold in polB backgrounds and returned to wild-type rates when polB cells were transduced to polB+. Following multiple passages of polB cells or prolonged starvation, a progressive loss of sensitivity to killing by peroxide was observed, suggesting that second-site suppressor mutations may be occurring with relatively high frequencies. The presence of suppressor mutations may account for the apparent lack of a mutant phenotype in earlier studies. A well-established polB strain, a dinA Mu d(Apr lac) fusion (GW1010), exhibited wild-type (Pol II+) sensitivity to killing by peroxide, consistent with the accumulation of second-site suppressor mutations. A high titer anti-Pol II polyclonal antibody was used to screen for the presence of Pol II in other bacteria and in the yeast Saccharomyces cerevisiae. Cross-reacting material was found in all gram-negative strains tested but was not detected in gram-positive strains or in S. cerevisiae. Induction of Pol II by nalidixic acid was observed in E. coli K-12, B, and C, in Shigella flexneri, and in Salmonella typhimurium.
Studying Complex Decision Making in Natural Settings: Using a Head-Mounted Video Camera to Study Competitive Orienteering

Head-mounted video recording is described as a potentially powerful method for studying decision making in natural settings. Most alternative data-collection procedures are intrusive and disruptive of the decision-making processes involved while conventional video-recording procedures are either impractical or impossible. As a severe test of the robustness of the methodology we studied the decision making of 6 experienced orienteers who carried a head-mounted light-weight video camera as they navigated, running as fast as possible, around a set of control points in a forest. Use of the Wilcoxon matched-pairs signed-ranks test indicated that compared with free recall, video-assisted recall evoked (a) significantly greater experiential immersion in the recall, (b) significantly more specific recollections of navigation-related thoughts and feelings, (c) significantly more realizations of map and terrain features and aspects of running speed which were not noticed at the time of actual competition, and (d) significantly greater insight into specific navigational errors and the intrusion of distracting thoughts into the decision-making process. Potential applications of the technique in (a) the environments of emergency services, (b) therapeutic contexts, (c) education and training, and (d) sports psychology are discussed.
Central Modulation of Pain Evoked From Myofascial Trigger Point

ObjectivesLow-intensity low-frequency electrostimulation delivered within a myofascial trigger point (MTP) has been used as intervention to deactivate MTPs. The therapeutic effect has been suggested to be due to peripheral mechanisms. However, nonpainful stimuli are also known to reduce simultaneous pain through central effects. The primary objective of the present study was to assess if central pain modulation occurs after intervention with low-intensity electrostimulation within an MTP. We hypothesized that intervention induces pain inhibition via the periaqueductal gray (PAG). MethodsTwenty-four patients with myofascial pain syndrome participated in the study. During functional magnetic resonance scanning, painful (high-intensity) intramuscular electrostimulation was delivered at random intervals (mean interstimulus interval=10.2 s) within an MTP of the upper left trapezius muscle. In-between scanning sessions, intervention (intramuscular electrostimulation, low-intensity, interstimulus interval=0.5 s) was applied to the same area. Patients were divided into responders and nonresponders according to their change in pressure pain thresholds relative to intervention. In addition to a whole brain search, a region of interest approach was also implemented to test the effect of intervention on PAG signal change. ResultsThe main findings were: (1) intervention modulated PAG activity to painful stimuli more in responders than in nonresponders, (2) change in PAG activity from the whole patient population correlated with change in pressure pain threshold, and (3) a network known to regulate affective qualities of the pain experience was (subsignificantly) engaged more in responders than in nonresponders. DiscussionThe applied intervention most likely involves supraspinal pain control mechanisms related to both antinociception and regulation of pain affect.
B Cell Depletion with Anti-CD79 mAbs Ameliorates Autoimmune Disease in MRL/lpr Mice1

MRL/lpr mice develop a spontaneous systemic lupus erythematosus-like autoimmune syndrome due to a dysfunctional Fas receptor, with contributions from other less well-defined genetic loci. The removal of B cells by genetic manipulation not only prevents autoantibody formation, but it also results in substantially reduced T cell activation and kidney inflammation. To determine whether B cell depletion by administration of Abs is effective in lupus mice with an intact immune system and established disease, we screened several B cell-specific mAbs and found that a combination of anti-CD79α and anti-CD79β Abs was most effective at depleting B cells in vivo. Anti-CD79 therapy started at 4–5 mo of age in MRL/lpr mice significantly decreased B cells (B220+CD19+) in peripheral blood, bone marrow, and spleens. Treated mice also had a significant increase in the number of both double-negative T cells and naive CD4+ T cells, and a decreased relative abundance of CD4+ memory cells. Serum anti-chromatin IgG levels were significantly decreased compared with controls, whereas serum anti-dsDNA IgG, total IgG, or total IgM were unaffected. Overall, survival was improved with lower mean skin scores and significantly fewer focal inflammatory infiltrates in submandibular salivary glands and kidneys. Anti-CD79 mAbs show promise as a potential treatment for systemic lupus erythematosus and as a model for B cell depletion in vivo.
Double quadrature bandpass sampling for a PLL and mixer-less low-IF multistandard receiver

This work presents a bandpass sampling receiver architecture, which takes the advantages of low IF approach while implementing downconversion and image rejection with-out mixer or frequency varying oscillators. The RF signal is downcoverted to a low-IF by means of a quadrature bandpass sampling. A fixed and reduced sampling frequency is applied. The second quadrature downconversion to baseband is accomplished using programmable gain amplifiers, which is done in discrete time domain by multiplying the signal with discrete cosine and sine waves of the IF. The system was modelled using ADS and validated with the downconversion and demodulation of an LTE standard signal. While quantizing the cosine and sine at IF1 with 6 bits, and excluding the noise, the output SNR was 58 dB. The obtained image rejection of the architecture for 6 bits was 46 dB.
Location of sites of transposon Tn916 insertion in the Mycoplasma mycoides genome

The genome of Mycoplasma mycoides subsp. mycoides GC1176-2 was cleaved into six large fragments by the endonuclease KpnI which also cleaved the transposon Tn916 once. This has allowed genomic mapping of insertion sites for 50 transformants of GC1176-2 containing Tn916. Almost all of the mapped sites were clearly separate. The transformants provide a bank of genomes each with a KpnI site at a different position to facilitate mapping of gene loci.
Critical exponents of the explosive percolation transition.

In a new type of percolation phase transition, which was observed in a set of nonequilibrium models, each new connection between vertices is chosen from a number of possibilities by an Achlioptas-like algorithm. This causes preferential merging of small components and delays the emergence of the percolation cluster. First simulations led to a conclusion that a percolation cluster in this irreversible process is born discontinuously, by a discontinuous phase transition, which results in the term "explosive percolation transition. " We have shown that this transition is actually continuous (second order) though with an anomalously small critical exponent of the percolation cluster. Here we propose an efficient numerical method enabling us to find the critical exponents and other characteristics of this second-order transition for a representative set of explosive percolation models with different number of choices. The method is based on gluing together the numerical solutions of evolution equations for the cluster size distribution and power-law asymptotics. For each of the models, with high precision, we obtain critical exponents and the critical point.
Phosphorylation of the flagellar regulatory protein FlrC is necessary for Vibrio cholerae motility and enhanced colonization

The human pathogen Vibrio cholerae specifically expresses virulence factors within the host, including cholera toxin (CT) and the toxin co‐regulated pilus (TCP), which allow it to colonize the intestine and cause disease. V. cholerae is a highly motile organism by virtue of a polar flagellum, and motility has been inferred to be an important aspect of virulence, yet the exact role of motility in pathogenesis has remained undefined. The two‐component regulatory system FlrB/FlrC is required for polar flagellar synthesis; FlrC is a σ54‐dependent transcriptional activator. We demonstrate that the transcriptional activity of FlrC affects both motility and colonization of V. cholerae. In a purified in vitro reaction, FlrB transfers phosphate to the wild‐type FlrC protein, but not to a mutant form in which the aspartate residue at amino acid position 54 has been changed to alanine (D54A), consistent with this being the site of phosphorylation of FlrC. The wild‐type FlrC protein, but not the D54A protein, activates σ54‐dependent transcription in a heterologous system, demonstrating that phospho‐FlrC is the transcriptionally active form. A V. cholerae strain containing a chromosomal flrCD54A allele did not synthesize a flagellum and had no detectable levels of transcription of the critical σ54‐dependent flagellin gene flaA. The V. cholerae flrCD54A mutant strain was also defective in its ability to colonize the infant mouse small intestine, approximately 50‐fold worse than an isogenic wild‐type strain. Another mutation of FlrC (methionine 114 to isoleucine; M114I) confers constitutive transcriptional activity in the absence of phosphorylation, but a V. cholerae flrCM114I mutant strain, although flagellated and motile, was also defective in its ability to colonize. The strains carrying D54A or M114I mutant FlrC proteins expressed normal levels of CT and TCP under in vitro inducing conditions. Our results show that FlrC ‘locked’ into either an inactive (D54A) or an active (M114I) state results in colonization defects, thereby demonstrating a requirement for modulation of FlrC activity during V. cholerae pathogenesis. Thus, the σ54‐dependent transcriptional activity of the flagellar regulatory protein FlrC contributes not only to motility, but also to colonization of V. cholerae.
On optimal hotspot selection and offloading

Devices like smart phones come with 3G/4G and WiFi radios, which creates possibilities of heterogeneous network access. We investigate scenarios where Internet access to a device is available only via the cellular network. However, not every user may connect directly to it. Users in the network may be split into hotspots and clients. Hotspots are the users that connect directly to the cellular network and may provide connectivity to the internet to other users by allowing them to connect to their WiFi interface. Clients connect to the cellular network only via hotspots. The optimization problem is to find the split of hotspots and clients, and the association between clients and hotspots, that maximizes the sum of the link rates of users. Importantly, the users must get at least the link rate they get when all are directly connected to the cellular network. In this paper, we formulate the optimization problem. We provide insights into the interplay of WiFi connectivity amongst users, their link rates to the cell tower, and the split that maximizes sum rate. We propose a novel heuristic approach to split the network. Median gains of 1.5× are observed over networks of up to 40 nodes.
Innovation from science: the role of network content and legitimacy ties

This study contributes to advance understanding on the micro-level foundations of the relationship between scientific research and innovation. We adopt a relational approach to scientific research networks through the analysis of the content of network ties, in contrast to more standard network approaches which are grounded on structural features of networks. We argue that the perceived legitimacy afforded through ties within research networks play a critical role in reconciling the conflicting logics of science and innovation. The proposed hypotheses are empirically tested in the context of the Spanish biomedical research system, drawing on a large scale survey of biomedical scientists. Our results indicate that the scientists’ acquisition of legitimacy through their research network play a critical role in the context of the translation from scientific research to technological achievements and innovations. Our findings also show that past scientific impact has a reinforcing effect on the relationship between legitimacy acquisition and technological achievements. On the contrary, we find that direct interaction with beneficiaries provides an alternative path to reconcile the conflicting logics of science and market, by compensating for the lack of acquired legitimacy from research network.
The Relationship Between Apoptosis of Endplate Chondrocytes and Aging and Degeneration of the Intervertebral Disc

Study Design. Apoptosis in cervical intervertebral disc cells and cartilaginous endplate cells was examined by the nick end labeling (TUNEL) technique during the process of natural aging and in a mouse experimental spondylosis model. Objectives. To determine the role of apoptosis in aging and degeneration of intervertebral discs by monitoring chronologic changes in the quantity and localization of apoptotic cells. Summary of Background Data. Apoptosis occurs within human intervertebral discs, but little is known about the pathologic significance of this process. On the other hand, the cartilaginous endplate is known to decrease in thickness and to disappear with aging and degeneration. The cause of this age-related change remains unclear. Methods. A mouse spondylosis model was prepared via surgical resection of the posterior spinal element in 12 mice to examine the experimentally induced spondylosis process. Eighteen naturally aged mice were also used to examine the influence of aging. Paraffin-embedded midsagittal sections of the cervical spine were obtained 2, 3, 6, and 12 months after surgery in the spondylosis model and in the age-matched naturally aged mice, as well as in 4-week-old and 18-month-old naturally aged mice. Sections were stained with hematoxylin and eosin, safranin-O, and the TUNEL procedure. The number of apoptotic cells and vital cells were counted in the cartilaginous endplate of the intervertebral disc excluding the growth cartilage, and the degree of disappearance of the cartilaginous endplate was evaluated. Results. Apoptosis, particularly noticeable in the cartilaginous endplate, increased with age and resulted in a marked decrease in cell density. Subsequently, the structure of the cartilaginous endplate began to disappear. Apoptosis was more evident and the structure of the cartilaginous endplate began to disappear more rapidly in the surgically treated group than in the naturally aged group. Conclusions. TUNEL-positive cells in the cartilaginous endplate increased with age, with destruction of the cartilaginous endplate after apoptosis (TUNEL-positive cell death). The application of the spondylosis model increased the incidence of apoptosis preceding the development of spondylosis. This suggests that apoptosis plays a role in the age-related changes seen in the cartilaginous endplate of the intervertebral disc and in the experimentally induced spondylosis process.
Fluorescence In Situ Hybridization Using 16S rRNA-Targeted Oligonucleotides Reveals Localization of Methanogens and Selected Uncultured Bacteria in Mesophilic and Thermophilic Sludge Granules

ABSTRACT 16S rRNA-targeted in situ hybridization combined with confocal laser scanning microscopy was used to elucidate the spatial distribution of microbes within two types of methanogenic granular sludge, mesophilic (35°C) and thermophilic (55°C), in upflow anaerobic sludge blanket reactors fed with sucrose-, acetate-, and propionate-based artificial wastewater. The spatial organization of the microbes was visualized in thin sections of the granules by using fluorescent oligonucleotide probes specific to several phylogenetic groups of microbes. In situ hybridization with archaeal- and bacterial-domain probes within granule sections clearly showed that both mesophilic and thermophilic granules had layered structures and that the outer layer harbored mainly bacterial cells while the inner layer consisted mainly of archaeal cells. Methanosaeta-,Methanobacterium-, Methanospirillum-, andMethanosarcina-like cells were detected with oligonucleotide probes specific for the different groups of methanogens, and they were found to be localized inside the granules, in both types of which dominant methanogens were members of the genusMethanosaeta. For specific detection of bacteria which were previously detected by whole-microbial-community 16S ribosomal DNA (rDNA)-cloning analysis (Y. Sekiguchi, Y. Kamagata, K. Syutsubo, A. Ohashi, H. Harada, and K. Nakamura, Microbiology 144:2655–2665, 1998) we designed probes specific for clonal 16S rDNAs related to unidentified green nonsulfur bacteria and clones related toSyntrophobacter species. The probe designed for the cluster closely related to Syntrophobacter species hybridized with coccoid cells in the inner layer of the mesophilic granule sections. The probe for the unidentified bacteria which were clustered with the green nonsulfur bacteria detected filamentous cells in the outermost layer of the thermophilic sludge granule sections. These results revealed the spatial organizations of methanogens and uncultivated bacteria and their in situ morphologies and metabolic functions in both mesophilic and thermophilic granular sludges.
Environmental control of invasin expression in Yersinia pseudotuberculosis is mediated by regulation of RovA, a transcriptional activator of the SlyA/Hor family

Invasin is the primary invasive factor of Yersinia pseudotuberculosis that allows efficient internalization into eukaryotic cells. We investigated invasin expression and found that the inv gene is regulated in response to a variety of environmental signals, such as temperature, growth phase, nutrients, osmolarity and pH, and requires the product of rovA, a member of the SlyA/Hor transcriptional activator family. The rovA gene was found by a genetic complementation strategy that restores temperature regulation of an unexpressed inv–phoA fusion in Escherichia coli K‐12. RovA plays a role in the invasion of Y. pseudotuberculosis into mammalian cells and mediates the regulation of invasin in response to all environmental signals analysed. Deletion analysis of the inv promoter region revealed a DNA segment extending 207 bp upstream of the transcriptional start site, which is required for maximal RovA‐induced inv transcription. Gel retardation assays showed that RovA interacts preferentially with this promoter fragment and suggested two potential RovA binding sites. Studies with chromosomal gene fusions also demonstrated that rovA follows the same pattern of regulation as invasin, indicating that environmental control of inv expression is mainly mediated by the control of RovA synthesis. Furthermore, we showed that a rovA–lacZ fusion is only slightly expressed in a rovA mutant strain, indicating that a positive autoregulatory mechanism is also involved in rovA expression.
A Statistical Learning Approach to Detect Abusive Twitter Accounts

The increased use of social media has motivated spammers to post their malicious activities on social network sites. Some of these spammers use adult content to further the distribution of their malicious activities. Moreover, the extensive number of users posting adult content in social media degrades the experience for other users for whom the adult content is not desired or appropriate. In this paper, we aim to detect abusive accounts that post adult content using Arabic language to target Arab speakers. There is limited natural language processing (NLP) resources for the Arabic language, and to the best of our knowledge no research has been done to detect adult accounts with Arabic language in social media. We used a statistical learning approach to analyze Twitter content to detect abusive accounts that use obscenity, profanity, slang, and swearing words in Arabic text format. Our approach achieved a predictive accuracy of 96% and overcomes imitations of the bag-of-word (BOW) approach.