Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.)

Fructose-1, 6-bisphosphate aldolase (FBA) is a key plant enzyme that is involved in glycolysis, gluconeogenesis, and the Calvin cycle. It plays significant roles in biotic and abiotic stress responses, as well as in regulating growth and development processes. In the present paper, 21 genes encoding TaFBA isoenzymes were identified, characterized, and categorized into three groups: class I chloroplast/plastid FBA (CpFBA), class I cytosol FBA (cFBA), and class II chloroplast/plastid FBA. By using a prediction online database and genomic PCR analysis of Chinese Spring nulli-tetrasomic lines, we have confirmed the chromosomal location of these genes in 12 chromosomes of four homologous groups. Sequence and genomic structure analysis revealed the high identity of the allelic TaFBA genes and the origin of different TaFBA genes. Numerous putative environment stimulus-responsive cis-elements have been identified in 1,500-bp regions of TaFBA gene promoters, of which the most abundant are the light-regulated elements (LREs). Phylogenetic reconstruction using the deduced protein sequence of 245 FBA genes indicated an independent evolutionary pathway for the class I and class II groups. Although, earlier studies have indicated that class II FBA only occurs in prokaryote and fungi, our results have demonstrated that a few class II CpFBAs exist in wheat and other closely related species. Class I TaFBA was predicted to be tetramers and class II to be dimers. Gene expression analysis based on microarray and transcriptome databases suggested the distinct role of TaFBAs in different tissues and developmental stages. The TaFBA 4–9 genes were highly expressed in leaves and might play important roles in wheat development. The differential expression patterns of the TaFBA genes in light/dark and a few abiotic stress conditions were also analyzed. The results suggested that LRE cis-elements of TaFBA gene promoters were not directly related to light responses. Most TaFBA genes had higher expression levels in the roots than in the shoots when under various stresses. Class I cytosol TaFBA genes, particularly TaFBA10/12/18 and TaFBA13/16, and three class II TaFBA genes are involved in responses to various abiotic stresses. Class I CpFBA genes in wheat are apparently sensitive to different stress conditions.
Development of a Web-Based Student Enquiry System: Integration of Ontology and CBR

This paper discusses the development o f a web-based student enquiry system using techniques in case-based reasoning combined with advances in ontology. The system functions as an online enquiry front-desk to allow prospective students to fin d out if they are eligible for admission to a post-graduate course in an Australian University. Ontology is applied in the system to ensure explicit specifications of concept and definition of terminology can be achieved in the web-based system. The system applies case-based reasoning technique to handle the query.
Perception and Modeling of Affective Qualities of Musical Instrument Sounds across Pitch Registers

Composers often pick specific instruments to convey a given emotional tone in their music, partly due to their expressive possibilities, but also due to their timbres in specific registers and at given dynamic markings. Of interest to both music psychology and music informatics from a computational point of view is the relation between the acoustic properties that give rise to the timbre at a given pitch and the perceived emotional quality of the tone. Musician and nonmusician listeners were presented with 137 tones produced at a fixed dynamic marking (forte) playing tones at pitch class D# across each instrument's entire pitch range and with different playing techniques for standard orchestral instruments drawn from the brass, woodwind, string, and pitched percussion families. They rated each tone on six analogical-categorical scales in terms of emotional valence (positive/negative and pleasant/unpleasant), energy arousal (awake/tired), tension arousal (excited/calm), preference (like/dislike), and familiarity. Linear mixed models revealed interactive effects of musical training, instrument family, and pitch register, with non-linear relations between pitch register and several dependent variables. Twenty-three audio descriptors from the Timbre Toolbox were computed for each sound and analyzed in two ways: linear partial least squares regression (PLSR) and nonlinear artificial neural net modeling. These two analyses converged in terms of the importance of various spectral, temporal, and spectrotemporal audio descriptors in explaining the emotion ratings, but some differences also emerged. Different combinations of audio descriptors make major contributions to the three emotion dimensions, suggesting that they are carried by distinct acoustic properties. Valence is more positive with lower spectral slopes, a greater emergence of strong partials, and an amplitude envelope with a sharper attack and earlier decay. Higher tension arousal is carried by brighter sounds, more spectral variation and more gentle attacks. Greater energy arousal is associated with brighter sounds, with higher spectral centroids and slower decrease of the spectral slope, as well as with greater spectral emergence. The divergences between linear and nonlinear approaches are discussed.
Prolonged Inhibition of Glioblastoma Xenograft Initiation and Clonogenic Growth following In Vivo Notch Blockade

Purpose: To examine the effects of clinically relevant pharmacologic Notch inhibition on glioblastoma xenografts. Experimental Design: Murine orthotopic xenografts generated from temozolomide-sensitive and -resistant glioblastoma neurosphere lines were treated with the γ-secretase inhibitor MRK003. Tumor growth was tracked by weekly imaging, and the effects on animal survival and tumor proliferation were assessed, along with the expression of Notch targets, stem cell, and differentiation markers, and the biology of neurospheres isolated from previously treated xenografts and controls. Results: Weekly MRK003 therapy resulted in significant reductions in growth as measured by imaging, as well as prolongation of survival. Microscopic examination confirmed a statistically significant reduction in cross-sectional tumor area and mitotic index in a MRK003-treated cohort as compared with controls. Expression of multiple Notch targets was reduced in the xenografts, along with neural stem/progenitor cell markers, whereas glial differentiation was induced. Neurospheres derived from MRK003-treated xenografts exhibited reduced clonogenicity and formed less aggressive secondary xenografts. Neurospheres isolated from treated xenografts remained sensitive to MRK003, suggesting that therapeutic resistance does not rapidly arise during in vivo Notch blockade. Conclusions: Weekly oral delivery of MRK003 results in significant in vivo inhibition of Notch pathway activity, tumor growth, stem cell marker expression, and clonogenicity, providing preclinical support for the use of such compounds in patients with malignant brain tumors. Some of these effects can persist for some time after in vivo therapy is complete. Clin Cancer Res; 19(12); 3224–33. ©2013 AACR.
Public Policy Involvement by Certified Health Education Specialists

Background. Advocating for health-related activities is an essential part of being a health educator and knowing how to advocate is an important part of the Certified Health Education Specialist (CHES) exam. However, based on previous research, there may be a gap between knowing how to and actually participating in public policy activities. The purpose of this study was to determine public policy involvement of CHES. Method. Surveys were mailed to a random sample of 500 CHES (41% response rate). Results. The highest selected public policy activities by CHES were the following: voting at an election (88.9%), contacting a public office (49%), and providing policy-related information (42.9%). The number of locations CHES individuals selected in having training on advocacy was a predictive factor in their confidence to perform advocacy. Positive correlations between perceived knowledge and effectiveness of the public policy process and self-reported involvement in public policy were found. Conclusion. Although there is moderate self-reported involvement in public policy activities, more emphasis needs to be placed on raising the confidence of CHES to perform advocacy-related activities in the field.
Highly Differentiated, Resting Gn-Specific Memory CD8+ T Cells Persist Years after Infection by Andes Hantavirus

In man, infection with South American Andes virus (ANDV) causes hantavirus cardiopulmonary syndrome (HCPS). HCPS due to ANDV is endemic in Southern Chile and much of Argentina and increasing numbers of cases are reported all over South America. A case-fatality rate of about 36% together with the absence of successful antiviral therapies urge the development of a vaccine. Although T-cell responses were shown to be critically involved in immunity to hantaviruses in mouse models, no data are available on the magnitude, specificity and longevity of ANDV-specific memory T-cell responses in patients. Using sets of overlapping peptides in IFN-γ ELISPOT assays, we herein show in 78 Chilean convalescent patients that Gn-derived epitopes were immunodominant as compared to those from the N- and Gc-proteins. Furthermore, while the relative contribution of the N-specific response significantly declined over time, Gn-specific responses remained readily detectable ex vivo up to 13 years after the acute infection. Tetramer analysis further showed that up to 16.8% of all circulating CD3+CD8+ T cells were specific for the single HLA-B*3501-restricted epitope Gn465–473 years after the acute infection. Remarkably, Gn465–473–specific cells readily secreted IFN-γ, granzyme B and TNF-α but not IL-2 upon stimulation and showed a ‘revertant’ CD45RA+CD27−CD28−CCR7−CD127− effector memory phenotype, thereby resembling a phenotype seen in other latent virus infections. Most intriguingly, titers of neutralizing antibodies increased over time in 10/17 individuals months to years after the acute infection and independently of whether they were residents of endemic areas or not. Thus, our data suggest intrinsic, latent antigenic stimulation of Gn-specific T-cells. However, it remains a major task for future studies to proof this hypothesis by determination of viral antigen in convalescent patients. Furthermore, it remains to be seen whether Gn-specific T cells are critical for viral control and protective immunity. If so, Gn-derived immunodominant epitopes could be of high value for future ANDV vaccines.
CAFE: an R package for the detection of gross chromosomal abnormalities from gene expression microarray data

Summary: The current methods available to detect chromosomal abnormalities from DNA microarray expression data are cumbersome and inflexible. CAFE has been developed to alleviate these issues. It is implemented as an R package that analyzes Affymetrix *. CEL files and comes with flexible plotting functions, easing visualization of chromosomal abnormalities. Availability and implementation: CAFE is available from https://bitbucket.org/cob87icW6z/cafe/ as both source and compiled packages for Linux and Windows. It is released under the GPL version 3 license. CAFE will also be freely available from Bioconductor. Contact: sander.h. bollen@gmail.com or nancy.mah@mdc-berlin.de Supplementary information: Supplementary data are available at Bioinformatics online.
A pattern-based approach for GUI modeling and testing

User Interface (UI) patterns are used extensively in the design of today's software. UI patterns embody commonly recurring solutions that solve common GUI design problems, such as “login,” “file-open,” and “search. ” Yet, testing of GUIs for functional correctness has largely ignored UI patterns. This paper formalizes the notion of a Pattern-Based Graphical User Interface (GUI) Testing method (PBGT) for systematizing and automating the GUI testing process. The space of all possible interactions with a GUI is typically very large. PBGT presents a new methodology to sample the input space using “UI Test Patterns,” that embody commonly recurring solutions to test GUIs. Our empirical studies show that the PBGT methodology is effective in revealing faults in fielded GUIs.
Pivotal estimation via square-root Lasso in nonparametric regression

We propose a self-tuning $\sqrt{\mathrm {Lasso}} $ method that simultaneously resolves three important practical problems in high-dimensional regression analysis, namely it handles the unknown scale, heteroscedasticity and (drastic) non-Gaussianity of the noise. In addition, our analysis allows for badly behaved designs, for example, perfectly collinear regressors, and generates sharp bounds even in extreme cases, such as the infinite variance case and the noiseless case, in contrast to Lasso. We establish various nonasymptotic bounds for $\sqrt{\mathrm {Lasso}} $ including prediction norm rate and sparsity. Our analysis is based on new impact factors that are tailored for bounding prediction norm. In order to cover heteroscedastic non-Gaussian noise, we rely on moderate deviation theory for self-normalized sums to achieve Gaussian-like results under weak conditions. Moreover, we derive bounds on the performance of ordinary least square (ols) applied to the model selected by $\sqrt{\mathrm {Lasso}} $ accounting for possible misspecification of the selected model. Under mild conditions, the rate of convergence of ols post $\sqrt{\mathrm {Lasso}} $ is as good as $\sqrt{\mathrm {Lasso}} $’s rate. As an application, we consider the use of $\sqrt{\mathrm {Lasso}} $ and ols post $\sqrt{\mathrm {Lasso}} $ as estimators of nuisance parameters in a generic semiparametric problem (nonlinear moment condition or $Z$-problem), resulting in a construction of $\sqrt{n}$-consistent and asymptotically normal estimators of the main parameters.
Supplementary Figures

Supplementary Figure 1. Schematic depiction of the spliceosomal snRNPs and the non-snRNP PRPF19:CDC5L protein complex at various assembly stages. The PRPF19:CDC5L complex association with the U12-dependent spliceosome is inferred from the major spliceosome (figure adapted from [1]).
“Caught between a scream and a hug”: Women’s perspectives on music listening and interaction with teenagers in the family unit

There has been considerable research on the emotional and cognitive impact of music in people’s everyday lives, but limited attention to its role within relationships, particularly inside the family unit. This article explores the emotional experiences of women in midlife when listening to music, with special reference to their interaction with the musical choices of their adolescent offspring and the bearing this has on their relationships. Ten women aged between 40 and 52 years and their teenage children provided qualitative data through semi-structured interviews which focused on their listening behaviours, expressions of self-identity and uses of music whilst considering each other’s musical tastes. This investigation employs a hermeneutic approach and provides new information about the relationship between adolescent musical habits and mother/child dynamics with particular reference to personal and social identity. Mothers were found to enjoy harmonious relationships with daughters through shared musical choices. This was not evident in the information exploring the relationship between mothers and their sons. The article reveals that adolescent musical habits play an important role in influencing the musical listening experiences of mothers.
Modelling voter behaviours by geographic information technology: a case of Hong Kong in 2004

Maps have been used widely to communicate election results and voter turnout. This article presents a practical approach to extracting and assembling the profiles of Hong Kong voters in the 2004 Legislative Council Election. It describes the use of modelling functions in a geographic information system to augment the examination of demographic and socio-economic constructs of voters and their voting behaviours. Voter profiles were used as predictors in forecasting election results. The predictive power of our model was assessed through visual and qualitative comparison with actual results of the election. The comparison showed that both maps exhibited similar patterns.
Bullying of children and adolescents with autism spectrum conditions: a ‘state of the field’ review

A ‘state of the field’ review of what is currently known about bullying of children and adolescents with autism spectrum conditions (ASC) is presented. We highlight compelling evidence that they are considerably more likely to be bullied than those with other or no special educational needs and disabilities. Although prevalence estimates vary from study to study, they are always worryingly high, with the highest rate reported as 94%. Those most at risk include (but are not limited to) individuals with Asperger syndrome and/or with milder deficits in social understanding, early adolescents, those attending mainstream school, and those with concurrent behavioural difficulties. Research on anti-bullying interventions for ASC is in its relative infancy. Currently available evidence suggests that a multi-level, comprehensive approach to intervention that offers parallel foci on children and young people with ASC, their peers, teaching and support staff, and the broader school ethos and climate is warranted. A crucial component of the above is the acknowledgement of the elevated risk experienced by those with ASC and the requirement to tailor interventions to their specific needs.
Attributes of Informative Disclosures

Regulatory actions and academic research suggest a variety of seemingly “good” disclosure attributes: readability, degree of forward-looking information, concreteness (numerical intensity), and more disclosure in general. While all of these attributes seem intuitively desirable, there is little empirical evidence about how these disclosure attributes compare in terms of influencing investors and analysts. We examine equity market responses to quarterly earnings announcements as a function of these disclosure attributes and find substantial variation in their effects. We find strong evidence that forward-looking disclosures represent informative disclosures, very little evidence that readability is associated with disclosure informativeness, and actually find evidence that both disclosure length and numerical intensity are negatively associated with disclosure informativeness. We discuss possible reasons for our results and conclude that controlling for underlying content is crucial in evaluating the desirability of textual attributes. Overall, our results should help inform both managers and regulators making decisions about how to craft or encourage informative disclosures. Our results also suggest caution for academics using seemingly intuitive measures of disclosure quality.
The Prevalence of Exposure to Domestic Violence Among High School Students in Tehran

Background: Domestic violence appears to be a major social problem. Researches in the last 10 years have uncovered multiple effects of witnessing domestic violence on children, ranging in severity from little or no effect to sever psychological harm. Objectives: This study aimed to measure the prevalence of exposure to domestic violence among high school students in Tehran. Materials and Methods: A cross-sectional survey was conducted on high school students of Tehran in the school year 2011–2012. The “Children’s Exposure to Domestic Violence Scale” was administered to a total cohort of 1,212 students (615 males and 597 females) selected by the stratified sampling method. Results: Approximately one-half of the participants (44.3%) had been exposed to their fathers’s violence against their mothers at least sometimes in their lives, the most common form of which was preventing the mother from doing something (28.5%) and the least common, hurting the mother with sharp or deadly tools (9.6%). A substantial proportion of the students (90.6%) had been exposed to violence in the community or at school, the most common kind would be being heard from someone calling another person names or making fun of them (81.7%) and the least common, being injured a child in the community or at school (31.8%). Conclusions: Exposure to violence is a widespread problem among children in Tehran. It encompasses a wide range and children were exposed to violence in different ways and forms.
Comparison of Surgical Skills in Laparoscopic and Robotic Tasks Between Experienced Surgeons and Novices in Laparoscopic Surgery: An Experimental Study

Purpose Robotic surgery is known to provide an improved technical ability as compared to laparoscopic surgery. We aimed to compare the efficiency of surgical skills by performing the same experimental tasks using both laparoscopic and robotic systems in an attempt to determine if a robotic system has an advantage over laparoscopic system. Methods Twenty participants without any robotic experience, 10 laparoscopic novices (LN: medical students) and 10 laparoscopically-experienced surgeons (LE: surgical trainees and fellows), performed 3 laparoscopic and robotic training-box-based tasks. This entire set of tasks was performed twice. Results Compared with LN, LEs showed significantly better performances in all laparoscopic tasks and in robotic task 3 during the 2 trials. Within the LN group, better performances were shown in all robotic tasks compared with the same laparoscopic tasks. However, in the LE group, compared with the same laparoscopic tasks, significantly better performance was seen only in robotic task 1. When we compared the 2 sets of trials, in the second trial, LN showed better performances in laparoscopic task 2 and robotic task 3; LE showed significantly better performance only in robotic task 3. Conclusion Robotic surgery had better performance than laparoscopic surgery in all tasks during the two trials. However, these results were more noticeable for LN. These results suggest that robotic surgery can be easily learned without laparoscopic experience because of its technical advantages. However, further experimental trials are needed to investigate the advantages of robotic surgery in more detail.
Variable 360° Vector-Sum Phase Shifter With Coarse and Fine Vector Scaling

A CMOS vector-sum phase shifter covering the full 360° range is presented in this paper. Broadband operational transconductance amplifiers with variable transconductance provide coarse scaling of the quadrature vector amplitudes. Fine scaling of the amplitudes is accomplished using a passive resistive network. Expressions are derived to predict the maximum bit resolution of the phase shifter from the scaling factor of the coarse and fine vector-scaling stages. The phase shifter was designed and fabricated using the standard 130-nm CMOS process and was tested on-wafer over the frequency range of 4.9-5.9 GHz. The phase shifter delivers root mean square (rms) phase and amplitude errors of 1.25° and 0.7 dB, respectively, at the midband frequency of 5.4 GHz. The input and output return losses are both below 17 dB over the band, and the insertion loss is better than 4 dB over the band. The circuit uses an area of 0.303 mm2 excluding bonding pads and draws 28 mW from a 1.2 V supply.