Miracle-dz/UniFAR-FTUs huggingface dataset:
Identifying target genes of the aryl hydrocarbon receptor nuclear translocator (Arnt) using DNA microarray analysis

Abstract The aryl hydrocarbon receptor nuclear translocator (Arnt) is a basic helix-loop-helix (bHLH) protein that also contains a Per-Arnt-Sim (PAS) domain. In addition to forming heterodimers with many other bHLH-PAS proteins, including the aryl hydrocarbon receptor (AhR) and hypoxia-inducible factors 1α, 2α and 3α, Arnt can also form homodimers when expressed from its cDNA in vitro or in vivo. However, target genes of the Arnt/Arnt homodimer remain to be identified. In this study, we have elucidated the profile of genes responsive to the reintroduction of Arnt expression in an Arnt-deficient mouse hepatoma cell line (c4), using DNA microarray analysis. The expression of 27 genes was upregulated by 1.5-fold or more in c4 cells infected with a retroviral vector expressing mouse Arnt, while no genes were found to be downregulated. Among the upregulated genes, BCL2/adenovirus E1B 19 kDa-interacting protein 1 (NIP3), serine (or cysteine) proteinase inhibitor, clade E, member 1 (PAI1), and N-myc downstream regulated-like (NDR1), were confirmed to be induced by Arnt using real-time PCR. We also found that the 5′ promoter region of 15 out of 20 upregulated genes contain the type 2 E-box 5′-CACGTG-3′ Arnt/Arnt binding sequence, consistent with the notion that they represent target genes for Arnt.
A study of community participation in solid waste management in Kathmandu metropolitan city

Solid waste management in Nepal has been one of the important and seriously discussed topics. Kathmandu Metropolitan City is unable to handle the solid waste by itself. So, community participation is being encouraged to manage the waste. In this way, my study will focus on community participation in solid waste management in Kathmandu. There are varieties of study areas for my research, Old Newar settlement (Ashan and Jyatha),Thamel (Tourism area), and Samakhuhsi Town Planning (ward no:-29 out of 38 wards of Kathmandu Metropolitan City.) Waste management in those areas has been managed through Ngos and Private sectors, communities, and Metropolitan Authority. Household questionnaire and interviews will be used as the research tools and a total of 48 respondents and 11 key respondents (2/2 senior employees ) from community organizations including 3 staffs from environment department of Kathmandu Metropolitan City respondents interviewed around the study areas. The study will use both qualitative and quantitative approach to analyze the information. It is believed that about 75% of the total waste generated in Kathmandu is of organic type which can be composted. The main aim of Solid Waste Management Practice is seen in all these settlements to segregate waste at source. All the communities have different approaches which the people follow in segregating waste. In all the settlements bins are provided. At Ashan and Local Newar Settlement area has a central collection system but the community members are asked to segregate waste. In Thamel too, it is a similar case but a private company is involved (directly) in managing waste. In Samakhushi Town Planning area, people are encouraged to manage waste in individual level in their own household. This Study will try to depict the status of solid waste management practices as well as the status of community participation community members in other activities such as recyclingreuse, disposal of waste, decision making within entire ward no 29 out of 38 wards of Kathmandu Metropolitan City
Clickstream tracking of TOR users: may be easier than you think

ABSTRACT As the importance and prevalence of web analytics have increased over the last decade, so has the number of user trying to maintain their online anonymity. The Onion Routing (TOR) system is often seen as the best anonymity tool out there and is used by nearly 2.5 million people daily. For a significant number of these users, many of TOR’s features and terms are rather difficult to comprehend; yet, these users tend to believe that TOR offers more privacy protection than what it is actually intended or able to provide. In this paper, the authors specifically focus on the TOR browser – one of the two key components of the TOR system. In particular, the authors demonstrate that if used in its default settings, the TOR browser provides little if any protection against four most common forms of user tracking. Hence, to achieve true online anonymity, extra efforts and vigilance need to be exercised on the part of the TOR user.
Incremental Community Mining in Location-based Social Network

A social network can be defined as a set of social entities connected by a set of social relations. These relations often change and differ in time. Thus, the fundamental structure of these networks is dynamic and increasingly developing. Investigating how the structure of these networks evolves over the observation time affords visions into their evolution structure, elements that initiate the changes, and finally foresee the future structure of these networks. One of the most relevant properties of networks is their community structure – set of vertices highly connected between each other and loosely connected with the rest of the network. Subsequently networks are dynamic, their underlying community structure changes over time as well, i.e they have social entities that appear and disappear which make their communities shrinking and growing over time. The goal of this paper is to study community detection in dynamic social network in the context of location-based social network. In this respect, we extend the static Louvain method to incrementally detect communities in a dynamic scenario following the direct method and considering both overlapping and non-overlapping setting. Finally, extensive experiments on real datasets and comparison with two previous methods demonstrate the effectiveness and potential of our suggested method.
A Literature Review on the Design of Enterprise Crowdsourcing Platforms

Crowdsourcing enables employers to outsource small tasks to an anonymous crowd over the internet. It is used for funding, generating new ideas, rating products, and also to outsource personnel resource consuming tasks. Enterprises that use such crowdsourcing process have found it beneficial for some tasks to restrict access to employees only. Therefore, enterprises require crowdsourcing platforms, to enable this kind of internal task processing. This paper explores the requirements for such processes by evaluating the scientific state of the art on enterprise crowdsourcing platforms. A structured literature review is presented with a focus on "enterprise crowdsourcing", which gives insights on scenarios and models, as well as elements and instruments of such platforms, leading to a refined model of enterprise crowdsourcing platforms.
Levonorgestrel-Releasing Intrauterine System for Women With Polycystic Ovary Syndrome

Polycystic ovary syndrome (PCOS) is related to clinical and metabolic comorbidities that may limit the prescription of combined hormonal contraceptives, with consequent need to use progestogen-only contraceptives (POCs). Thus, the objective of the present study was to evaluate the clinical and metabolic effects of a POC, the levonorgestrel-releasing intrauterine system (LNG-IUS), in women with PCOS followed up over a period of 6 months compared to baseline and to women without PCOS. Thus, an observational, prospective, controlled study was conducted on 30 women with a diagnosis of PCOS who presented adverse effect secondary to the use of combined oral contraceptives (nausea, headache, mastalgia or vomiting; PCOS group) paired with 30 ovulatory women without PCOS (control group), both groups being free of comorbidities and having chosen the LNG-IUS as contraceptive. Clinical, laboratory, and ultrasonographic variables were evaluated immediately before LNG-IUS insertion and 6 months after the use of this method. Before LNG-IUS insertion, the PCOS group had higher total testosterone levels (P = .04), lower HDL levels (P = .04), and greater ovarian volume (P < .01) than the control group. Six months after LNG-IUS insertion, there was a 2.3% increase in abdominal circumference (P = .04) and a 3.4% increase in fasting glycemia (P = .02). On the other hand, mean ovarian volume was 10% smaller compared to the volume found before LNG-IUS insertion (P = .04), LDL levels were reduced by 5.2% (P = .03), and total cholesterol levels were reduced by 6.7% (P < .01) compared to baseline evaluation in the PCOS group. The remaining variables did not differ significantly during the 6 months of observation. The control group did not show significant changes compared to the period before LNG-IUS insertion. When the groups were compared after the 6-month follow-up, only glycemia showed a statistically significant variation between the groups, with glycemia levels increasing by 3.4% in the PCOS group and decreasing by 2.6% in the control group (P = .008). In conclusion, the use of the LNG-IUS for 6 months was not associated with relevant changes in clinical or metabolic variables of women with no comorbidities regardless of the presence of PCOS.
Commande sans capteur des moteurs synchrones à aimants permanents par injection de signaux

Cette these etudie la problematique du fonctionnement sans capteur et a basse vitesse des moteurs synchrones a aimant permanent par l'injection des signaux. Nous nous focalisons sur les effets de la saturation magnetique car leur compensation est primordiale pour resoudre cette problematique. Nous proposons une methode originale pour modeliser la saturation magnetique en utilisant une approche energetique (les formulations Lagrangienne et Hamiltonienne), ou les symetries physiques sont exploitees pour simplifier l'expression de l'energie magnetique. Les donnees experimentales montrent qu'un polynome de degre 4 est suffisant pour decrire avec precision les effets de la saturation. Ensuite, nous proposons une analyse claire et originale basee sur la moyennisation de second ordre et qui explique comment obtenir l'information de position a partir de l'injection des signaux (en utilisant le modele propose). Nous donnons une relation explicite entre les oscillations des courants statoriques et la position du rotor; cette relation est utilisee en temps reel. Ce modele de saturation magnetique ainsi que la procedure d'estimation de position ont ete testes et valides sur deux types de moteurs : avec des aimants permanents a l'interieur ou sur la surface du rotor. Les resultats experimentaux obtenus sur un banc de test montrent que les erreurs d'estimation de la position du rotor n'excedent pas quelques degres electriques dans la zone d'operation a basse vitesse.
“Thinking Aloud”: Data Collection in the Natural Setting

Knowledge of how nurses make decisions is a desirable outcome of research. However, there currently exists an inadequacy in the techniques used to examine such decision making. In this article, the authors describe the techniques used in two studies incorporating “thinking aloud” to successfully examine the decision making of expert critical care practitioners in the natural setting. Both techniques of thinking aloud were found to provide useful information regarding decision making in the natural setting. No ethical implications were experienced in conducting these studies in the natural setting. In conclusion, the use of thinking aloud in the natural setting is an effective means of data collection.
Office Management of COPD in Primary Care: A 2009 Clinical Update

Abstract Primary care physicians (PCPs) usually are the first to diagnose and care for patients with chronic obstructive pulmonary disease (COPD). This article discusses key teaching points of recently published guidelines for PCPs and the common challenges of treating COPD patients in a primary care setting, and clarifies common misconceptions. First, PCPs should choose one of the published screening tools to assess for COPD. Spirometry is a useful tool to confirm the diagnosis of COPD to distinguish COPD from asthma, to stage the disease for determining treatment, and it can help with smoking cessation efforts. Chest radiographs do not help to diagnose COPD but are useful to rule out other causes of dyspnea and cough. Differentiating COPD from asthma is important because it affects treatment decisions and prognosis. Second, the goals of COPD treatment are to relieve symptoms, improve exercise tolerance, prevent exacerbations, and improve quality (although not necessarily extend quantity) of life. Chronic obstructive pulmonary disease can be treated at any stage, and the treatments are adjusted in a stepwise approach based on disease severity. Third, as part of the long-term management of COPD, smoking cessation should be discussed at every visit, and inhaler technique should be reviewed at regular intervals. Chronic obstructive pulmonary disease patients should also receive the recommended influenza and pneumococcal vaccinations. Primary care physicians are in a unique position to identify COPD patients early, to implement primary and secondary preventive measures, and to provide care that addresses the full spectrum of COPD and its comorbidities.
Complete Genome Sequence of the Cellulose-Degrading Bacterium Cellulosilyticum lentocellum

ABSTRACT Cellulosilyticum lentocellum DSM 5427 is an anaerobic, endospore-forming member of the Firmicutes. We describe the complete genome sequence of this cellulose-degrading bacterium, which was originally isolated from estuarine sediment of a river that received both domestic and paper mill waste. Comparative genomics of cellulolytic clostridia will provide insight into factors that influence degradation rates.
Study on the Impact of Customer Loyalty and Experiential Value on Value Co-creation Based on Sharing Economy

The study goes along the idea from the customer perception to the establishment of emotional and behavioral tendencies, to analyze the value co-creation mechanism in sharing economy for realizing the value maximum provide guidance for maximizing the value in the process of business management. It finds that the customer loyalty positively mediates the relationship between experiential value and value co-creation. The self-efficacy and proactive personality positively moderate the relationship between customer loyalty and customer participation behavior.
Activity-Dependent Long-Term Depression of Electrical Synapses

Paired bursting in coupled neurons depresses electrical synapses while their asymmetry increases after unidirectional use. Use-dependent forms of synaptic plasticity have been extensively characterized at chemical synapses, but a relationship between natural activity and strength at electrical synapses remains elusive. The thalamic reticular nucleus (TRN), a brain area rich in gap-junctional (electrical) synapses, regulates cortical attention to the sensory surround and participates in shifts between arousal states; plasticity of electrical synapses may be a key mechanism underlying these processes. We observed long-term depression resulting from coordinated burst firing in pairs of coupled TRN neurons. Changes in gap-junctional communication were asymmetrical, indicating that regulation of connectivity depends on the direction of use. Modification of electrical synapses resulting from activity in coupled neurons is likely to be a widespread and powerful mechanism for dynamic reorganization of electrically coupled neuronal networks.
Actigraphy to measure day structure as a therapeutic variable in the treatment of schizophrenic patients

Objective: A component of social skills is the ability to adapt to the social rhythms of the environment. Patients with schizophrenia are often disabled in this adaptation. Thus, structuring activities throughout the day has long been known as part of psychosocial treatments. Actigraphy as a tool to measure acitvity and circadian rhythms may even serve as an indicator for the day‐structuring of schizophrenic patients.
Abdominal Lymph Node Metastasis in Patients With Non-Small-Cell Lung Cancer as Shown by PET/CT

Purpose The aim of this study is to investigate the frequency and the spread of abdominal lymph node metastasis in patients with non-small-cell lung cancer (NSCLC) by 18F-FDG PET/CT. Patients and Methods Retrospective evaluation of the 18F-FDG PET/CT examinations of 1191 patients diagnosed with NSCLC was performed. The metastatic abdominal lymph nodes of the patients were classified as inside the routine imaging field (covering the field of chest CT including adrenal glands) and outside the field. Results Seventy-four patients (6 F, 68 M; mean: 61 ± 11 years old) among 1191 patients (6%) were identified to have abdominal lymph node metastases. These abdominal lymph node metastasis changed management in 10 out of 74 patients (14%), and there were lymph node metastases outside the routine conventional imaging field in 43 (58%) patients. Conclusion 18F-FDG PET/CT provided identification of the distant metastatic lymph nodes out of conventional imaging field in more than half of NSCLC patients with abdominal metastasis which changed patient management in 14% of the patients due to abdominal lymph node metastasis outside the routine imaging field. This study shows the necessity of imaging NSCLC patients with an imaging protocol with larger imaging field like PET/CT.
An Analysis of Playing Positions in Elite Men's Volleyball: Considerations for Competition Demands and Physiologic Characteristics

Sheppard, JM Gabbett, TJ, and Reeberg Stanganelli, L-C. An analysis of playing positions in elite men's volleyball: considerations for competition demands and physiologic qualities. J Strength Cond Res 23(6): 1858-1866, 2009-The purpose of this study was to investigate the physiologic demands, physiologic characteristics, and jumping ability of different playing positions in elite male volleyball players. The first investigation involved an analysis of 16 international men's volleyball matches. The second investigation involved an analysis of the anthropometric and jump performance characteristics of 142 Development National Team (DNT) and Senior National Team (SNT) international volleyball players. Mean (±SD) frequency of block jumps for Middles (11.00 ± 3.14) was significantly greater than for Setters (6.25 ± 2.87, p < 0.001) and Outsides (6.50 ± 3.16, p < 0.001). Attack jumps were performed more frequently by Middles (7.75 ± 1.88), and this was found to be significantly more than for Setters (0.38 ± 1.06, p < 0.001) and Outsides (5.75 ± 3.25, p < 0.01). Middles were taller than Outsides and Setters (p < 0.001). Consequently, Middles had a significantly higher reach and greater body mass than Outsides (p < 0.001, p < 0.003) and Setters (p < 0.001, p < 0.001). Both Middles and Outsides had superior countermovement vertical jump (CMVJ) and spike jump (SPJ) scores compared with Setters (p < 0.001). Position-specific comparisons between DNT players and SNT players demonstrated that the SNT players were superior in relative CMVJ and SPJ scores (p < 0.05), with a large magnitude of effect (d > 0.99). The results of this study highlight the large jumping and landing demands placed on the taller and heavier players in the middle position. In addition to establishing the magnitude of difference in jumping ability between junior and senior national team players, the results also provide a comprehensive data set that may assist with talent identification and talent development for aspiring male volleyball players.
Nucleic Acid-based Methods for Early Detection of Sepsis

© 2017 Annals of Cardiac Anaesthesia | Published by Wolters Kluwer Medknow The Editor, The primary determinant of survival in patients that develop sepsis is the time for recognition of infection and initiation of antibiotics. [1] The culture of an organism, still the gold standard, takes nearly 48–72 h to grow and test the antibiotic susceptibility of organism by conventional methods. [2] Nucleic acid‐based methods give results within 6–12 h and are also available for commercial use. In a broad sense, all pathogenic organisms have a unique internal genomic sequence which can be amplified using primers of DNA or RNA and later cross‐referenced with the available signatures in the database to identify the genus or species of the microorganism present in a sample. Following tests can be applied on the whole‐blood samples and are available commercially: • SepsiTest (Molzym, Bremen, Germany): Uses broad range polymerase chain reaction (PCR) to amplify the species specific internal regions in the genome of a pathogen. If found positive, these amplicons are subject to sequence analysis to identify the microorganism. The broad range binding of primers to 16s and 18s ribosomal RNA allows detection of more than 345 pathogens by SepsiTest. [3] In India, it is being used in the name of SepsiScreen (SRL Lab)[4] • Lightcycler SeptiFast test (Roche Molecular Systems, Branchburg, NJ, USA): Uses multiplex real‐time PCR technology to identify internal transcribed spacer region and distinguishes 25 different species of bacteria and fungi, including Staphylococcus, Streptococcus, Enterococcus, Enterobacter, Pseudomonas, Acinetobacter, Klebsiella, Escherichia coli, Proteus, Serratia, Candida spp. , and Aspergillus fumigatus. It takes up to 6 h for a final report and is approved for use in Europe. It is one of the most extensively used sepsis screen tests[3,5] • Vyoo (SIRS‐Lab, Jena, Germany): Uses multiplex PCR technology with subsequent gel electrophoresis. Vyoo can detect 34 bacteria, 7 fungi, and 5 antibiotic resistance genes in about 7 h. In addition to the organisms detected by SeptiFast, it also detects Clostridium, Haemophilus, Neisseria, and Prevotella spp[3,6] • Magicplex sepsis test (Seegene, Korea): Magicplex also uses multiplex PCR technology to detect ninety sepsis causing pathogens in 3 h after DNA extraction. Seventy gram positive, 12 gram negative, and six types of fungi may be identified using this test. It also detects three genes for antibiotic resistance[7] • IRIDICA (Abbott Laboratories, USA): IRIDICA Nucleic Acid-based Methods for Early Detection of Sepsis
Representation frequency transforms by matrix direct sum

Spectral methods are widely used to analyzed the different dimensions signals. Wavelet transform, Discrete cosine transform, Fourier transform and its windowed version are the basis of modern software and hardware for digital signal processing. This article considers issues of formation the modified scheme wavelet transform in matrix form. Showed examples for data processing based on the Haar and Daubechies wavelets giving an idea of the difference between traditional and modified approaches to the method implementation. Further, this approach is extended to the Windowed Fourier transform. Traditional and proposed matrix forms represented. The modified method has minimal effect on coefficient values in depending direction of processing in all cases.
Molecular cloning of copper resistance genes from Pseudomonas syringae pv. tomato

A cosmid library of copper-resistant (Cur) Pseudomonas syringae pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases), conferred copper resistance. The PT23 Cur gene(s) was located on pCOP1 by subcloning PstI restriction endonuclease fragments of pCOP1 in the broad-host-range vector pRK404. A subclone containing a 4.4-kilobase PstI fragment conferred Cur on PS61. The Cur gene(s) was further located by insertional inactivation with Tn5. A subcloned fragment internal to the Cur determinant on pCOP2 was probed to plasmid and chromosomal DNA of four copper-resistant and three copper-sensitive strains of P. syringae pv. tomato. The probe hybridized to plasmids in resistant strains, but showed no detectable homology to copper-sensitive strains.