Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of glycogen in thin sections of amphibian embryos.

Embryonic amphibian cells when examined with the electron microscope were observed to contain an abundance of small particles, approximately 325 A in diameter. The periodic acid/Schiff reaction and enzymic digestion were employed to determine the nature of the particles, and from the results of these tests they were concluded to be glycogen. Treatment of thin sections with periodic acid/lead citrate solutions resulted in a marked increase in contrast of the glycogen particles compared with other cell structures, and in a clearly defined substructure of 40-A grains appearing within the particles. This differential staining method enabled the particulate glycogen to be distinguished from ribosomes.
Gearing up for a hunt?: A Great Bend aspect cache from central Kansas

The Johnson cache is a collection of 65 chipped stone artifacts, all made from varieties of Permian chert, which were found in the bank of Cow Creek in Rice County, central Kansas. The artifacts include large bifacial cores, triangular arrow point preforms, blade-like end-scraper blanks, and a few flake tools. This collection is interpreted to represent essential elements of Great Bend aspect people’s chipped stone technological system which was placed into storage for potential future use, but which was never reopened and used. The cache is thought to date between AD 1200 and 1600.
Electrode Potentials of Hermidin, the Chromogen of Mercurialis perennis.

HAAS and HILL [1925, 1] have recently directed attention to a chromogen of considerable interest which escapes from the plasmolysed cells of Mercurialis perennis. If the coarsely divided tissue be steeped, anaerobically, in chloroform water for a short time, there results an extract which, on exposure to oxygen, assumes first a rich blue and then a yellow-brown colour. These investigators [1925, 2] have further shown that, within the experimental limits imposed by the difficulty of arresting the oxidation exactly at the stage of maximum intensity of blue colour, the two successive reactions are attended by an equal absorption of oxygen. The oxidation to the blue substance (cyanohermidin) was demonstrated to be readily reversible and Haas and Hill [1926] were further persuaded that the second stage of oxidation is also, at least partially, reversible, although they were unable quantitatively or consistently to effect the reduction of the yellow compound (chrysohermidin) through the blue to the chromogen. The data presented in the papers cited suggested that the systems involved might be amenable to electrometric study and the present paper reports the measure of success attained. The application of electrometric methods to organic systems has been extensively discussed in its theoretical aspects by Clark and Cohen [1923, 1]. The general relations may be summarised in a form of the Nernst electrode equation E =E'o RT log[St]
A multiple Lyapunov function approach to stabilization of fuzzy control systems

This paper addresses stability analysis and stabilization for Takagi-Sugeno fuzzy systems via a so-called fuzzy Lyapunov function which is a multiple Lyapunov function. The fuzzy Lyapunov function is defined by fuzzily blending quadratic Lyapunov functions. Based on the fuzzy Lyapunov function approach, we give stability conditions for open-loop fuzzy systems and stabilization conditions for closed-loop fuzzy systems. To take full advantage of a fuzzy Lyapunov function, we propose a new parallel distributed compensation (PDC) scheme that feedbacks the time derivatives of premise membership functions. The new PDC contains the ordinary PDC as a special case. A design example illustrates the utility of the fuzzy Lyapunov function approach and the new PDC stabilization method.
E2F1‐dependent pathways are involved in amonafide analogue 7‐d‐induced DNA damage, G2/M arrest, and apoptosis in p53‐deficient K562 cells

The E2F1 gene well known is its pivotal role in regulating the entry from G1 to S phase, while the salvage antitumoral pathway which implicates it, especially in the absence of p53, is not fully characterized. We therefore attempted to identify the up‐ and down‐stream events involved in the activation of the E2F1‐dependent pro‐apoptotic pathway. For this purpose, a amonafide analogue, 7‐d (2‐(3‐(2‐(Dimethylamino)ethylamino)propyl)‐6‐(dodecylamino)‐1H‐benzo[de]isoquinoline‐1,3(2H)‐dione) was screened, which exhibited high antitumor activity against p53‐deficient human Chronic Myelogenous Leukemia (CML) K562 cells. Analysis of flow cytometry and western blots of K562 cells treated with 7‐d revealed an appreciable G2/M cycle arrest and apoptosis in a dose and time‐dependent manner via p53‐independent pathway. A striking increase in “Comet tail” formation and γ‐H2AX expression showed that DNA double strand breaks (DSB) were caused by 7‐d treatment. ATM/ATR signaling was reported to connect E2F1 induction with apoptosis in response to DNA damage. Indeed, 7‐d‐induced G2/M arrest and apoptosis were antagonized by ATM/ATR signaling inhibitor, Caffeine, which suggested that ATM/ATR signaling was activated by 7‐d treatment. Furthermore, the increased expression of E2F1, p73, and Apaf‐1 and p73 dissociation from HDM2 was induced by 7‐d treatment, however, knockout of E2F1 expression reversed p73, Apaf‐1, and p21Cip1/WAF1 expression, reactivated cell cycle progression, and inhibited 7‐d‐induced apoptosis. Altogether our results for the first time indicate that 7‐d mediates its growth inhibitory effects on CML p53‐deficient cells via the activation of an E2F1‐dependent mitochondrial and cell cycle checkpoint signaling pathway which subsequently targets p73, Apaf‐1, and p21Cip1/WAF1. J. Cell. Biochem. 113: 3165–3177, 2012. © 2012 Wiley Periodicals, Inc.
Transient power supply voltage (V/sub DDT/) analysis for detecting IC defects

Transient power supply voltage (V/sub DDT/) analysis is a new testing technique demonstrated as a powerful alternative and complement to I/sub DDQ/ testing. V/sub DDT/ analysis takes advantage of the limited response time of a voltage supply to the changing power demand of an IC during operation. Changes in the V/sub DD/ response time can be used to detect increases in the power demand of a microcontroller with resolutions of 20 nA at 100 kHz, 1 /spl mu/A at 1 MHz, and 2.5 /spl mu/A at 1.5 MHz. These current sensitivities have been shown for ICs with very low I/sub DDQ/ (<100 nA) and for an IC with an intrinsic I/sub DDQ/>300 /spl mu/A. The present system uses 100 cycle averaging to compensate for low frequency "jitter". The V/sub DDT/ signal acquisition protocols, frequency versus sensitivity tradeoffs, hardware considerations, noise limitations, data examples, and areas for future research are described.
Quadband planar PCB antenna for WLAN and mobile WiMAX applications

A quad band planar microstrip-line-fed printed circuit board (PCB) antenna for different applications is introduced in this paper. The proposed antenna can be used in mobile applications such as GSM 900 and DCS-1800. It can be also used for Bluetooth and wireless local area network (WLAN) applications at 2.4GHz. The antenna has good characteristics at 2.3 GHz and 3.5 GHz which enable it to be used for mobile WiMAX 2.3–2.4GHz applications and WiFi 2.4–2.45GHz applications. The antenna is built on RT/Duroid 5880 high frequency laminate with a finite ground plane on the other side of the substrate. Simulation results show that the antenna has good performance in all frequency bands of operation.
Sequence of mouse adenovirus type 1 DNA encoding the amino terminus of protein IVa2

The nucleotide sequence which is predicted to encode the amino terminus of the mouse adenovirus type 1 (MAV-1) IVa2 gene was determined as previously described (1). The sequence reported here, 13.7-16.5 map units of MAV-1, abuts the previously reported nucleotide sequence predicted to encode the carboxy terminus of the FVa2 gene (1). The IVa2 mRNA is predicted to be transcribed from right to left; the bottom strand corresponds to the protein coding sequence. The predicted amino acid (aa) sequence has 54% identity and 81% similarity to the human Ad2 IVa2 sequence over the entire length of the IVa2 protein sequence (448 aa) (2). The start codon is underlined and the elipsis indicates continuation of the protein sequence toward the C terminus. We propose that like Ad2 IVa2 mRNA, the MAV-1 mRNA is spliced, with an intron from nt 4850-4563, as indicated.
Laparoscopic conservative treatment of colo-vesical fistula: a new surgical approach.

INTRODUCTION The standard treatment of colo-vesical fistula is the exeresis of fistula, suture of bladder wall, colic resection with or without temporary colostomy. Usually the approach is open because conversion rates and morbidity are lower than laparoscopy. The aim of video is to show the steps of a new mini-invasive approach of colo-vesical fistula without colic resection. MATERIALS AND METHODS A 69 years old male underwent laparoscopic conservative treatment of colo-vesical fistula due to endoscopic polipectomy in sigmoid diverticulum. 12 mm trocar for the camera was placed at the umbilicus, two 10 mm trocars were placed along bisiliac line and 5 mm port was placed along left emiclavear line; Trendelenburg position was 20°. The fistulous loop was carefully isolated, clipped with Hem-o-lock clips and removed. Since diverticular disease appeared slight and no inflammation signs were evident, colon resection was not performed. We sutured and sinked the sigmoid wall; after curettage of the fistula site, the bladder wall was sutured. Fat tissue was placed between sigmoid and bladder wall to reduce the risk of fistula recurrence. RESULTS Operative time, estimated blood loss, catheterization time, time to flatus and hospital stay were respectively 210 minutes, 300 mL, 10 days, 48 h and 8 days. The histological examination showed colonic inflammatory and necrotic tissue. No complications or fistula recurrence occurred at 54 months follow-up. CONCLUSIONS The laparoscopic conservative treatment of colo-vesical fistula is a safe and feasible technique, in particular when the diverticular disease is limited and the fistula is not due to diverticulitis.
Continuing utilisation of specialised health services in extremely preterm infants

Objective To compare healthcare use from neonatal discharge to 18 months corrected age (CA) of two groups of extremely preterm neonates (23–25 vs 26–28 weeks). Design Cohort study. Patients Infants born at ≤28 weeks and admitted in three hospital centres in Quebec, Canada (n=254). Main outcome measures Neurodevelopmental outcomes and healthcare use from neonatal discharge to 18 months CA. Results Re-hospitalisation rates occurred in 57% of children born at 23–25 weeks and in 49% of those born at 26–28 weeks. In these two age groups, by 18 months, 61% vs 59% were followed in physical or occupational therapy, 29% vs 17% were enrolled in a long-term rehabilitation program, 38% vs 28% used prescribed medication, and 59% vs 33% required medical assistive devices, respectively. Risk of re-hospitalisation was related to bronchopulmonary dysplasia (BPD), severe brain injury, use of home oxygen or an apnoea monitor and older age at neonatal discharge. Multiple births, BPD, severe brain injury, older age at neonatal discharge and single parenthood were associated with risk of using out-patient health services above average (>2 services). Conclusion Extremely preterm children are frequently re-hospitalised during infancy and use a substantial amount of healthcare resources. These results highlight the importance of resource allocation to preterm infants for medical and rehabilitation services after discharge from the neonatal intensive care unit.
Physical and functional interaction between hepatitis C virus NS5A protein and ovarian tumor protein deubiquitinase 7B

Hepatitis C virus (HCV) NS5A protein plays crucial roles in viral RNA replication, virus assembly, and viral pathogenesis. Although NS5A has no known enzymatic activity, it modulates various cellular pathways through interaction with cellular proteins. HCV NS5A (and other HCV proteins) are reportedly degraded through the ubiquitin–proteasome pathway; however, the physiological roles of ubiquitylation and deubiquitylation in HCV infection are largely unknown. To elucidate the role of deubiquitylation in HCV infection, an attempt was made to identify a deubiquitinase (DUB) that can interact with NS5A protein. An ovarian tumor protein (OTU), deubiquitinase 7B (OTUD7B), was identified as a novel NS5A‐binding protein. Co‐immunoprecipitation analyses showed that NS5A interacts with OTUD7B in both Huh‐7 and HCV RNA replicon cells. Immunofluorescence staining revealed that HCV NS5A protein colocalizes with OTUD7B in the cytoplasm. Moreover, HCV infection was found to enhance the nuclear localization of OTUD7B. The OTUD7B‐binding domain on NS5A was mapped using a series of NS5A deletion mutants. The present findings suggest that the domain I of NS5A is important and the region from amino acid 121 to 126 of NS5A essential for the interaction. Either V121A or V124A mutation in NS5A disrupts the NS5A‐OTUD7B interaction. The results of this in vivo ubiquitylation assay suggest that HCV NS5A enhances OTUD7B DUB activity. Taken together, these results suggest that HCV NS5A protein interacts with OTUD7B, thereby modulating its DUB activity.
Prevalence and Risk Factors for Peripheral Artery Disease among Patients on Maintenance Peritoneal Dialysis

Background: Cardiovascular disease is common in end-stage renal disease patients and accounts for more than half of the mortalities. Peripheral artery disease (PAD) is frequently found in this population and is an independent predictor of cardiovascular mortality. However, there have been limited studies reporting the prevalence and risk factors for PAD among patients on maintenance continuous ambulatory peritoneal dialysis (CAPD) therapy. Methods: 343 CAPD patients from our clinic were recruited, and PAD was diagnosed using a value of ankle-brachial index <0.9. Both traditional and dialysis-related factors were evaluated. Results: The prevalence of PAD was 27.4% in all patients, which increased to 45% in patients aged >70 years. In age- and gender-adjusted logistic regression, diabetes mellitus (OR 2.354, 95% CI 1.288–4.3), diastolic blood pressure (OR 0.964, 95% CI 0.939–0.989), extracellular/intracellular water ratio (OR 2.504, 95% CI 1.067–5.804), and lnCRP (OR 1.251, 95% CI 1.031–1.518) were independently associated with PAD. Conclusion: Our study suggested that PAD is a common problem in patients on maintenance peritoneal dialysis, especially in elderly dialysis subjects. Diabetes mellitus, elevated hsCRP level, and extracellular/intracellular water ratio are all independent risk factors for PAD. It also suggested that aggressive risk factor management for PAD should be undertaken in CAPD patients.
Child Soldier: When boys and girls are used in war

Child Soldier: When Boys and Girls Are Used in War is the true story of Michel Chikwanine. Michel is like many other five-yearolds: he has a loving family and spends his days going to school and playing soccer. But in 1993, the democratic republic of Congo, where Michel and his family live, is a country in tumult. one afternoon Michel and his friends are kidnapped by rebel militants and forced to become child soldiers. Child Soldier is the sometimes heartbreaking but ultimately inspiring true story of the triumph of the human spirit. Co-authored by Jessica dee humphreys and illustrated in graphic-novel format by Claudia dávila (the Future According to luz series, Super red riding hood), Child Soldier deftly and delicately tackles a very difficult subject matter.
Using grey prediction theory to reduce handoff overhead in cellular communication systems

A novel handoff algorithm based on grey theory is being proposed for reducing the handoff overhead in cellular communication environment. The design of the methods is intended to be used under a log-normal fading environment. The grey prediction model is used to predict the received signal strength indicator (RSSI) values from two base stations (BS). The decision whether performing handoff is based on the predictive RSSI value, the received RSSI value and n-1 past RSSI values. By changing the handoff probability of two regions (divided by the middle point of two separated BSs), the position of first handoff to be executed will close to the middle point of two separated BSs. The simulation results show that the proposed algorithm with grey theory obtains excellent performance because it not only minimizes the number of performing handoff, but also has a very short calculating time.
Stability of Hybrid Dissipative Hamiltonian Systems

This paper investigates the stability of hybrid dissipative Hamiltonian systems under (arbitrary) switching laws, and proposes a number of new results for the problem. For the case that the switching law is an infinite-valued piecewise right-continuous constant function, under a realistic assumption, it is shown that hybrid dissipative Hamiltonian system is stable under arbitrary switching laws. Based on this and by using the dissipative Hamiltonian structural properties and zero-state detectibility/observability, several sufficient conditions are presented for the asymptotical stability of the hybrid dissipative Hamiltonian system. As for the case that the switching law is related only to the state, some new stability results are proposed for the hybrid dissipative Hamiltonian system. Finally, as an application, the results obtained in this paper are applied to investigate the stability of ordinary hybrid nonlinear systems, and several useful corollaries are obtained for the systems. Study on an example and numerical simulations shows that the results obtained in this paper are very practicable in analyzing the stability of explicit hybrid systems.
Effects of a high‐fat, low‐versus high‐glycemic index diet: retardation of insulin resistance involves adipose tissue modulation

Beneficial effects of low glycemic index (GI) diets in rodents have been studied using healthy low‐fat diets, while the effects might be different on high‐fat diets inducing progression of insulin resistance. We fed C57BL/6J male mice high‐fat low/ high‐GI (LGI/HGI) diets for 13 wk. Glucose and insulin tolerance and serum substrates, including adipokines, were measured longitudinally. The LGI group showed a significantly higher glucose tolerance from wk 2 onwards, which was supported by lower serum insulin and free fatty acids levels at 8 wk, and a tendency for lower leptin levels, while resistin levels remained similar. At 11 wk, when differences in serum resistin started to increase, differences in serum insulin were diminished. Although food intake was similar throughout the study, body weights and epididymal adipose tissue mass became significantly lower in the LGI group at necropsy. Several serum substrates and adipose tissue leptin mRNA levels, as analyzed by Q‐PCR, were, again, significantly lower, whereas adiponectin mRNA levels were higher. Taken together, an LGI high‐fat diet maintains higher glucose tolerance and insulin sensitivity via adipose tissue modulation solely because of a difference in the type of carbohydrate, supporting a nutritional approach in the fight against insulin resistance. —Van Schothorst, E. M. , Bunschoten, A. , Schrauwen, P., Mensink, R. P., Keijer, J. Effects of a high‐fat, low versus high glycemic index diet: retardation of insulin resistance involves adipose tissue modulation. FASEB J. 23, 1092–1101 (2009)
Alveolar Epithelial Ion and Fluid Transport cAMP regulation of Cl and HCO 3 secretion across rat fetal distal lung epithelial cells

We isolated and cultured fetal distal lung epithelial (FDLE) cells from 17- to 19-day rat fetuses and assayed for anion secretion in Ussing chambers. With symmetrical Ringer solutions, basal short-circuit currents (I sc ) and transepithelial resistances were 7.9 ± 0.5 μA/cm 2 and 1,018 ± 73 Ω. cm 2 , respectively (means ± SE; n = 12). Apical amiloride (10 μM) inhibited basal I sc by ∼50%. Subsequent addition of forskolin (10 μM) increased I sc from 3.9 ± 0.63 μA/cm 2 to 7.51 ± 0.2 μA/cm 2 (n = 12). Basolateral bumetanide (100 μM) decreased forskolin-stimulated I sc from 7.51 ± 0.2 μA/cm 2 to 5.62 ± 0.53, whereas basolateral 4,4'-dinitrostilbene-2,2'-disulfonate (5 mM), an inhibitor of HCO - 3 secretion, blocked the remaining I sc . Forskolin addition evoked currents of similar fractional magnitudes in symmetrical Cl - - or HCO - 3 -free solutions; however, no response was seen using HCO - 3 - and Cl - -free solutions. The forskolin-stimulated I sc was inhibited by glibenclamide but not apical DIDS. Glibenclamide also blocked forskolin-induced I sc across monolayers having nystatin-permeablized basolateral membranes. Immunolocalization studies were consistent with the expression of cystic fibrosis transmembrane conductance regulator (CFTR) protein in FDLE cells. In aggregate, these findings indicate the presence of cAMP-activated Cl - and HCO - 3 secretion across rat FDLE cells mediated via CFTR.
An Iris Outline Tracker: For Various Eye Shapes in a Single Video Camera without Infrared Illumination

This paper describes a sophisticated method to track irises in a monocular video sequence without infrared illumination. In order to reduce the influence of eyelids and to cope with the variance of the appearance of eyes of different people, we propose a variable circular sector separability filter (VCSSF), and use it to estimate the likelihood of hypotheses in the particle filter. We confirmed that our iris outline tracker has the ability of tracking various eyes for different people whose head motion including translation, 3D rotation and zoom, and even when they are wearing glasses. Through the comparative experiments of the VCSSF, the conventional circular separability filter (CCSF) and the ellipse separability filter (ESF), we confirmed that our iris tracking algorithm is more robust than others.
Techniques for identifying cross‐disciplinary and ‘hard‐to‐detect’ evidence for systematic review

Driven by necessity in our own complex review, we developed alternative systematic ways of identifying relevant evidence where the key concepts are generally not focal to the primary studies' aims and are found across multiple disciplines—that is, hard‐to‐detect evidence. Specifically, we sought to identify evidence on community engagement in public health interventions that aim to reduce health inequalities. Our initial search strategy used text mining to identify synonyms for the concept ‘community engagement’. We conducted a systematic search for reviews on public health interventions, supplemented by searches of trials databases. We then used information in the reviews' evidence tables to gather more information about the included studies than was evident in the primary studies' own titles or abstracts. We identified 319 primary studies cited in reviews after full‐text screening. In this paper, we retrospectively reflect on the challenges and benefits of the approach taken. We estimate that more than a quarter of the studies that were identified would have been missed by typical searching and screening methods. This identification strategy was highly effective and could be useful for reviews of broad research questions, or where the key concepts are unlikely to be the main focus of primary research. Copyright © 2013 John Wiley & Sons, Ltd.
A research on transmission of message and voice using CCNx

In this paper, we studied for the transmission of message and voice using CCNx that is one of the Content Centric Network(CCN) projects. CCN is considered as a way to solve the explosive growth of Internet traffic. In order to apply CCN to the common services that are widely used, we developed applications that send message and voice. The application can operate without a separate server by using CCNx. Using mobile devices such as a raspberry pie, we tested in an environment similar to the actual environment. In this result, we can confirm that CCN is applicable to the general services.