Miracle-dz/UniFAR-FTUs huggingface dataset:
Combined Effects of Mugwort Herb and Vitamin C on Shelf-Life of Vacuum-Packed Seasoned Pork

This study was performed to investigate the possibility of the addition of mugwort herb extract (MH) and vitamin C (VC) alone (0.05%) and in combination (0.05% each) on shelf-life of seasoned pork. The combination of VC+MH demonstrated a significant reduction in thiobarbituric acid reactive substances, and volatile basic nitrogen in seasoned pork. Also, the pH values, total lactic acid concentration, lactic acid bacteria count, and the sensory properties (discoloration, flavor, and overall acceptability) of seasoned pork were not significantly affected by adding MH and/or VC. All seasoned pork were rejected by sensory panel when LAB count reached levels of 5-6 Log CFU/g, TLA concentration has been above a level of 3.6-3.9 mg lactic acid/g, and pH values ranged from 5.31-5.51 (15 d). Therefore, the findings showed that spoilage of seasoned pork does not appear to be the result of lipid oxidation, but is caused by lactic acid producing bacteria which result in sour odor.
Plaque-hyaluronidase-responsive high-density-lipoprotein-mimetic nanoparticles for multistage intimal-macrophage-targeted drug delivery and enhanced anti-atherosclerotic therapy

Increasing evidence has highlighted the pivotal role that intimal macrophage (iMΦ) plays in the pathophysiology of atherosclerotic plaques, which represents an attractive target for atherosclerosis treatment. In this work, to address the insufficient specificity of conventional reconstituted high-density lipoprotein (rHDL) for iMΦ and its limited cholesterol efflux ability, we designed a hyaluronan (HA)-anchored core–shell rHDL. This nanoparticle achieved efficient iMΦ-targeted drug delivery via a multistage-targeting approach, and excellent cellular cholesterol removal. It contained a biodegradable poly (lactic-co-glycolic acid) (PLGA) core within a lipid bilayer, and apolipoprotein A-I (apoA-I) absorbing on the lipid bilayer was covalently decorated with HA. The covalent HA coating with superior stability and greater shielding was favorable for not only minimizing the liver uptake but also facilitating the accumulation of nanoparticles at leaky endothelium overexpressing CD44 receptors in atherosclerotic plaques. The ultimate iMΦ homing was achieved via apoA-I after HA coating degraded by hyaluronidase (HAase) (abundant in atherosclerotic plaque). The multistage-targeting mechanism was revealed on the established injured endothelium–macrophage co-culture dynamic system. Upon treatment with HAase in vitro, the nanoparticle HA-(C)-PLGA-rHDL exhibited a greater cholesterol efflux capacity compared with conventional rHDL (2.43-fold). Better targeting efficiency toward iMΦ and attenuated liver accumulation were further proved by results from ex vivo imaging and iMΦ-specific fluorescence localization. Ultimately, HA-(C)-PLGA-rHDL loaded with simvastatin realized the most potent anti-atherogenic efficacies in model animals over other preparations. Thus, the HAase-responsive HDL-mimetic nanoparticle was shown in this study to be a promising nanocarrier for anti-atherogenic therapy, in the light of efficient iMΦ-targeted drug delivery and excellent function of mediating cellular cholesterol efflux.
Structural Basis for the cAMP-dependent Gating in the Human HCN4 Channel*

Hyperpolarization-activated cAMP-regulated (HCN) channels play important physiological roles in both cardiovascular and central nervous systems. Among the four HCN isoforms, HCN2 and HCN4 show high expression levels in the human heart, with HCN4 being the major cardiac isoform. The previously published crystal structure of the mouse HCN2 (mHCN2) C-terminal fragment, including the C-linker and the cyclic-nucleotide binding domain (CNBD), has provided many insights into cAMP-dependent gating in HCN channels. However, structures of other mammalian HCN channel isoforms have been lacking. Here we used a combination of approaches including structural biology, biochemistry, and electrophysiology to study cAMP-dependent gating in HCN4 channel. First we solved the crystal structure of the C-terminal fragment of human HCN4 (hHCN4) channel at 2.4 Å. Overall we observed a high similarity between mHCN2 and hHCN4 crystal structures. Functional comparison between two isoforms revealed that compared with mHCN2, the hHCN4 protein exhibited marked different contributions to channel function, such as a ∼3-fold reduction in the response to cAMP. Guided by structural differences in the loop region between β4 and β5 strands, we identified residues that could partially account for the differences in response to cAMP between mHCN2 and hHCN4 proteins. Moreover, upon cAMP binding, the hHCN4 C-terminal protein exerts a much prolonged effect in channel deactivation that could have significant physiological contributions.
Working Within the Context of Three-Tiered Models of Prevention: Using Schoolwide Data to Identify High School Students for Targeted Supports

This article addressed 3 objectives for assisting the research and teaching communities in developing 3-tiered models of prevention at the high school level. The authors first established the benefit of using systematic behavior screening tools within the context of 3-tiered models to identify and support high school students with academic and behavioral challenges. Second, they offered a structure for linking students demonstrating need with secondary (Tier 2) supports. Third, they provided illustrations of systematic approaches to detect high school students for whom primary prevention efforts were inadequate according to academic and behavioral data. The authors conclude by offering considerations for school psychologists assisting school-site teams with implementing, monitoring, and sustaining these procedures as part of regular school practices.
Mining social media streams to improve public health allergy surveillance

Allergies are one of the most common chronic diseases worldwide. One in five Americans suffer from either allergy or asthma symptoms. With the prevalence of social media, people sharing experiences and opinions on personal health symptoms and concerns on social media are increasing. Mining those publicly available health related data potentially provides valuable healthcare insights. In this paper, we propose a real-time allergy surveillance system that first classifies tweets to identify those that mention actual allergy incidents using bag-of-words model and NaiveBayesMultinomial classifier and applies in-depth text and spatiotemporal analysis. Our experimental results show that the proposed system can detect predominant allergy types with high precision and that allergy-related tweet volume is highly correlated to the weather data (daily maximum temperature). We believe that this is the first study that examines a large-scale social media stream for in-depth analysis of allergy activities.
Rrb1p, a Yeast Nuclear WD-Repeat Protein Involved in the Regulation of Ribosome Biosynthesis

ABSTRACT Ribosome biogenesis is regulated by environmental cues that coordinately modulate the synthesis of ribosomal components and their assembly into functional subunits. We have identified an essential yeast WD-repeat-containing protein, termed Rrb1p, that has a role in both the assembly of the 60S ribosomal subunits and the transcriptional regulation of ribosomal protein (RP) genes. Rrb1p is located in the nucleus and is concentrated in the nucleolus. Its presence is required to maintain normal cellular levels of 60S subunits, 80S ribosomes, and polyribosomes. The function of Rrb1p in ribosome biogenesis appears to be linked to its association with the ribosomal protein rpL3. Immunoprecipitation of Rrb1p from nuclear extracts revealed that it physically interacts with rpL3. Moreover, the overproduction of Rrb1p led to increases in cellular levels of free rpL3 that accumulated in the nucleus together with Rrb1p. The concentration of these proteins within the nucleus was dependent on ongoing protein translation. We also showed that overexpression of RRB1 led to an increase in the expression of RPL3 while all other examined RP genes were unaffected. In contrast, depletion of RRB1 caused an increase in the expression of all RP genes examined except RPL3. These results suggest that Rrb1p regulates RPL3 expression and uncouples it from the coordinated expression of other RP genes.
Second language learning in elementary and high school students

Abstract A cross-sectional approach covering an age range of 10 years was used to compare the developmental changu in picture naming md number naming of 77 high scbool md 74 elementary students who came from Germany to Sweden. For the two different groups of students, length of residcoce in Swedcn was the main independent variable and reaction time on simple naming tasks of pictures and numbers in German and Swedish was the dependent variable. The results provide evidence that elementuy school students achieved a balanced form of bilingualism 2 years earlier than high school students on the picture naming task. Naming two-digit numbers was shown to be a relatively difficult task for elementary school children, as indiated by markedly prolonged response times. Despite this fact, after about 4 years of residence in Sweden, both groups of students reached language ballaced on this task in that they showed identical response times in both languages. The results on the picture naming task were taken as support.
Loop nucleotides control primary and mature miRNA function in target recognition and repression

MicroRNA (miRNA) genes produce three major RNA products; primary (pri-), precursor (pre-), and mature miRNAs. Each product includes sequences complementary to cognate targets, thus they all can in principle interact with the targets. In a recent study we showed that pri-miRNAs play a direct role in target recognition and repression in the absence of functional mature miRNAs. Here we examined the functional contribution of pri-miRNAs in target regulation when full-length functional miRNAs are present. We found that pri-let-7 loop nucleotides control the production of the 5’ end of mature miRNAs and modulate the activity of the miRNA gene. This insight enabled us to modulate biogenesis of functional mature miRNAs and dissect the causal relationships between mature miRNA biogenesis and target repression. We demonstrate that both pri- and mature miRNAs can contribute to target repression and that their contributions can be distinguished by the differences between the pri- and mature miRNAs’ sensitivity to bind to the first seed nucleotide. Our results demonstrate that the regulatory information encoded in the pri-/pre-miRNA loop nucleotides controls the activities of pri-miRNAs and mature let-7 by influencing pri-miRNA and target complex formation and the fidelity of mature miRNA seed generation.
US OPHTHALMIC REVIEW 93 Scleritis — Infectious Versus Inflammatory

S cleritis refers to a heterogeneous group of diseases characterized by a severe painful inflammatory process of the sclera, that may also involve the cornea, adjacent episclera, and underlying uveal tract. It is associated with significant ophthalmic and systemic morbidity. Scleritis sometimes occurs in an isolated fashion, without evidence of inflammation in other organs. However, in up to 50% of patients, scleritis is associated with an underlying systemic illness such as rheumatoid arthritis or granulomatosis with polyangiitis. Infection is an important but rare cause of scleritis, occurring in about 5–10% of all cases. Due to the similarity of its presentation, infectious scleritis is often initially managed as autoimmune, potentially worsening its outcome. Careful clinical history taking (including history of ocular surgery), detailed ocular examination, appropriate investigation for ocular disease with or without underlying systemic disease, and timely intervention has improved the long-term outcome for patients with this disease.
Resilient software defined networking for industrial control networks

Software Defined Networking (SDN) is currently a hot topic in the area of Datacenter Networking or Enterprise Networks as it has the promise to radically simplify network management and operation. However, it has not been considered so far as a promising candidate for Industrial Control Networks mainly because of the deterministic performance requirements and the dedicated design of those networks to fulfil strict performance guarantees. In this paper, we propose a resilient SDN based architecture for Industrial Control Networks and show that by combining several SDN based fast failover technologies using per-link Bidirectional Forwarding Detection (BFD), preconfigured primary and backup paths and flexible packet duplication orchestrated by an SDN controller, we can reduce significantly the control latency and provide more stringent performance guarantees even under lossy and failing links.
Planning and Designing Web- Based Electronic Commerce: a case study in the insurance industry

Electronic Commerce (EC) comprises new ways of doing business with customers, suppliers and other business partners. EC is enabled by information and communication technology, in particular the World Wide Web (in short, the Web). While numerous organisations have started to use the Web as an interface to clients and business partners, many have been disappointed by lower than expected response rates and higher than expected costs. One reason for non profitable Web based EC applications is that they do not reflect the strategic preconditions of EC. This lack may be due to deficits in the process of developing an EC-strategy for their Web application. Furthermore, there is a void of instruments and tools to support this process. In order to fill this void, this paper proposes two basic frameworks for planning a Web based EC application. The application of these frameworks is illustrated by the example of a medium seized insurance company. Based on experiences drawn from the case study, the proposed frameworks will be evaluated.
Human-Organoid Models: Accomplishments to Salvage Test-Animals

Late stage attritions in drug discovery are costly and consuming. Improbable response of test molecules acquired in non-human systems is attributed to be the major cause of clinical failures. While conventional in vitro methods of drug discovery do not truly represent the human system, the animal models used for in vivo validation are also genetically and phenotypically distant from humans. However, recent developments in organoid culture are motivating and elevate hopes for replacing test animals with artificial human tissue models. Possibility of creating functional tissue ex vivo has a potential to revolutionize the way human therapeutics is perceived. Not only will it bridge the gap between drug development and its clinical efficacy but also help strategizing regenerative medicine. Successful human-tissue surrogates would liberate test animals or at least minimize their use for research purposes. Potential drug candidates tested on human-tissue equivalents are expected to generate clinically much more relevant data. Here we deliberate upon the options and possibilities of accomplishing human organoid models for in vitro testing and their significance in therapeutics.
Components of working memory predict symptoms of distress

Working memory (WM) is a cognitive system that allows us to select, organise, and integrate perceptual information with memories and current goal-directed intentions. As such, this system is central to day-to-day functioning and would be expected to be especially important in decision making and problem solving. We hypothesised that to the extent that individuals differ in WM capacity they would also be differentially vulnerable to the experience of depression and anxiety. Undergraduate students completed a computerised change detection task in which storage capacity and filtering efficiency measures were obtained. It was found that low WM storage and poor filtering efficiency interacted to predict higher levels of emotional distress as measured by self-reported depressive symptoms, ruminative coping, and state anxiety. It was suggested that low capacity and a poor ability to ignore task-irrelevant information may lead to poorer problem solving, more extreme judgements of oneself and circumstances, increased rumination, and poorer emotional regulation.
Alpine dams. From hydroelectric power to artificial snow

Les barrages-reservoirs de montagne ont ete realises initialement dans les Alpes pour repondre a la demande d’energie en periode hivernale. Une certaine diversification des usages de l’eau s’est ensuite progressivement developpee, en relation avec le developpement touristique des collectivites locales. Aujourd’hui, la participation des ouvrages d’Electricite De France a la production de neige de culture represente une nouvelle etape. Dans les regions ou les amenagements hydroelectriques sont nombreux, les besoins en eau pour la production de neige peuvent etre resolus par prelevements a partir des adductions EDF. Les gestionnaires de stations echappent ainsi aux inconvenients lies a la construction et a la gestion des « retenues collinaires ». Cette evolution, qui concerne deja quelques regions alpines comme la haute Maurienne ou le Beaufortin, apparait comme une forme renouvelee d’integration territoriale de la ressource en eau.
The internal dynamics of power-sharing in Africa

Given the increasing use of power-sharing arrangements to manage a wide range of political crises over the past five years it is more important than ever to turn a critical eye on the dynamics and outcomes of unity governments. This paper argues that two key factors shape the way that power-sharing functions in Africa: the distribution of violence (that is, whether any one party has a monopoly on victimhood or whether all parties have committed, and retain the capacity to commit, atrocities) and the level of elite cohesion (whether political leaders have developed norms of mutual accommodation that render it easier to find areas of common-ground). The first half of the paper identifies four main power-sharing dynamics in Africa based on different combinations of the distribution of violence and the level of elite cohesion: the politics of distrust, the politics of collusion, the politics of partisanship and the politics of pacting. The second-half of the paper then draws on evidence from Angola, Burundi, the DRC, Kenya, South Africa and Zimbabwe to illustrate how such variations in the practice of power-sharing shape the prospects for reform.
RNS2: a senescence-associated RNase of Arabidopsis that diverged from the S-RNases before speciation.

Several self-compatible species of higher plants, such as Arabidopsis thaliana, have recently been found to contain S-like RNases. These S-like RNases are homologous to the S-RNases that have been hypothesized to control self-incompatibility in Solanaceous species. However, the relationship of the S-like RNases to the S-RNases is unknown, and their roles in self-compatible plants are not understood. To address these questions, we have investigated the RNS2 gene, which encodes an S-like RNase (RNS2) of Arabidopsis. Amino acid sequence comparisons indicate that RNS2 and other S-like RNases make up a subclass within an RNase superfamily, which is distinct from the subclass formed by the S-RNases. RNS2 is most similar to RNase LE [Jost, W. , Bak, H. , Glund, K. , Terpstra, P., Beintema, J. J. (1991) Eur. J. Biochem. 198, 1-6. ], an S-like RNase from Lycopersicon esculentum, a Solanaceous species. The fact that RNase LE is more similar to RNS2 than to the S-RNases from other Solanaceous plants indicates that the S-like RNases diverged from the S-RNases prior to speciation. Like the S-RNase genes, RNS2 is most highly expressed in flowers, but unlike the S-RNase genes, RNS2 is also expressed in roots, stems, and leaves of Arabidopsis. Moreover, the expression of RNS2 is increased in both leaves and petals of Arabidopsis during senescence. Phosphate starvation can also induce the expression of RNS2. On the basis of these observations, we suggest that one role of RNS2 in Arabidopsis may be to remobilize phosphate, particularly when cells senesce or when phosphate becomes limiting.
A governança regional em turismo: realidade? Estudo de caso sobre o destino Estrada Real

During the last two decades the concept of regional governance has been spread all around the world as a democratic and innovative model of managing. Meanwhile, in the field of tourism the use of this concept is still embedded in myths and incongruence. This study aims at analyzing the effectiveness of the governance models applied in the development of the tourism in Brazil based on a case study of the Estrada Real Program. A survey with the professionals enrolled with the tourist sector of the southeast macro-region of the country, where this Program is implemented, supported the research. The conclusion point to problems related to the planning, implementation and follow-up of the Program, showing a low degree of understanding among the professionals of the concept of regional governance and their operational obstacles and possibilities of strengthening.
Conducting Interaction: Patterns of Behavior in Focused Encounters

Preface 1. Introduction 2. Some context for Context Analysis: a view of the origins of structural studies of face-to-face interaction 3. Some functions of gaze direction in two-person conversation 4. Movement co-ordination in social interaction 5. Some functions of the face in a kissing round 6. A description of some human greetings 7. Spatial organisation in social encounters: the F-formation system 8. Behavioural foundations for the process of frame-attunement in face-to-face interaction List of films cited References Index.
Content and Contagion in Yawning

Yawning has a well documented contagious effect: viewing or hearing a yawn—as well as talking or thinking about yawns—causes human subjects to yawn. While comparative ethological and neurological accounts suggest that yawning is a function of primitive biological structures in the brain stem, these analyses do not account for infectious yawning caused by representational and semantic states. Investigating the relationship between perceptual and cognitive avenues of yawn induction affords a unique opportunity to examine how higher level cognitive faculties interact with involuntary or automated processing systems. In this paper, I examine three distinct attempts to reconcile the cognitive properties of contagious yawning with its physiological basis—one neurological, one philosophical, and one functional. None of these accounts are unproblematic, and the most plausible hypothesis for the evolution of contagious yawning does not satisfactorily explain the cognitive iterations of the phenomenon. I argue that the most likely explanation of the contagion links perceptual elements of witnessed yawns to conceptual representations. More centrally, this kind of integrated account has repercussions for general theories of human thought and rationality, and suggests that higher level representational states engage neurophysiological structures in determining human behavior.