Miracle-dz/UniFAR-FTUs huggingface dataset:
Novel transcription map for the B19 (human) pathogenic parvovirus

The B19 parvovirus, a small single-stranded DNA virus of 5.4 kilobases, is pathogenic in humans. B19 has remarkable specificity for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those of other animal parvoviruses. However, B19 transcription had unusual features in comparison with that in other animal parvoviruses. At least nine overlapping poly(A)+ transcripts were identified in infected cells; all but one contained large introns. B19 differed from other parvoviruses in the initiation of all transcripts at a strong left side promoter (p6) and the absence of a functional internal promoter; the presence of short 5' leader sequences of about 60 bases and very large introns for RNAs encoded by the right side of the genome; two separate transcription termination sites, in contrast to cotermination at the far right side of the genome for other parvoviruses; the probable utilization by three transcripts of a variant polyadenylation signal (ATTAAA or AATAAC) in the middle of the genome; and the abundance of two unique transcripts from the middle of the genome which did not code for capsid proteins. The unusual transcription map of B19 suggests that regulation of the relative abundance of transcripts occurs by splicing and termination-polyadenylation events rather than by promoter strength. In combination with the published nucleotide sequence, the novel transcription map separated the pathogenic B19 virus at a molecular level from other animal parvoviruses and human adeno-associated virus.
An Asset–Liability Version of the Capital Asset Pricing Model with a Multi-Period Two-Fund Theorem

Despite the simplicity of its strategic asset allocation policy prescription, the original Two-Fund Theorem has never been used by practitioners. The authors present a new capital asset pricing model (CAPM) that incorporates investors’ deferred spending plans, or “economic liabilities”—the underlying purpose behind all investments—and thus reveal a new risk-free asset, the investor’s liability-matching asset portfolio. In combination with a slightly redefined world market portfolio of risky assets, the authors’ model forms a new two-fund theorem that is sensible, practical, and usable. The revised theorem provides an accessible and tractable form of an intertemporal CAPM, bridging the large gap between the simplistic single-period CAPM of Sharpe and others and the difficult, complex intertemporal models of Merton and others.
A Split Staphylococcus aureus Cas9 as a Compact Genome-Editing Tool in Plants

Split-protein methods—where a protein is split into two inactive fragments that must re-assemble to form an active protein—can be used to regulate the activity of a given protein and reduce the size of gene transcription units. Here, we show that a Staphylococcus aureus Cas9 (SaCas9) can be split, and that split-SaCas9 expressed from Agrobacterium can induce targeted mutagenesis in Nicotiana benthamiana. Since SaCas9 is smaller than the more commonly used Cas9 derived from Streptococcus pyogenes, the split-SaCas9 provides the smallest tool yet for clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) plant genome editing. Both sets of split-SaCas9 (_430N/431C and _739N/740C) exhibited genome-editing activity, and the activity of split-SaCas9_739N/740C was almost the same as that of full-length SaCas9. This result indicates that split-SaCas9_739N/740C is suitable for use in targeted mutagenesis. We also show that the split-SaCas9 fragment expressed from Tomato mosaic virus could induce targeted mutagenesis together with another fragment expressed from Agrobacterium, suggesting that a split-SaCas9 system using a plant virus vector is a promising tool for integration-free plant genome editing. Split-SaCas9 has the potential to regulate CRISPR/Cas9-mediated genome editing activity in plant cells both temporally and spatially.
Research ethics and journalism in the academy: Identifying and resolving a conflict of culture

The difficulty faced by research ethics committees in evaluating ethical conduct in journalism can be considered a recent conundrum. Journalistic investigation has traditionally been seen as residing outside the need for ethics clearances owing to debates around its status as research and to the reluctance of journalism practitioners to subject their investigations to committee evaluation. The inclusion of creative industries in revamped definitions of research, however, means that if journalistic inquiry is to be tallied under national research reward schemes, it must be ethically accountable. This article interrogates the difficulties caused by a conflict of cultures between ethics committees and journalism research and poses a number of possible resolutions. It aims to function as an exploration of key thinking in the field, thus acting as a frame for further development of case-specific examples of the issues raised.
The physical activity recall assessment for people with spinal cord injury: validity.

PURPOSE This study examined the construct validity of the physical activity recall assessment for people with spinal cord injury (PARA-SCI). METHODS First, to assess convergent validity, relationships between PARA-SCI scores and measures of aerobic fitness and muscular strength were examined among 73 men and women with SCI. Second, extreme groups analyses were conducted. PARA-SCI scores from 158 people with SCI were compared between groups differing on demographic, disability, and behavioral characteristics. RESULTS Scores from the leisure time physical activity (LTPA) and cumulative activity PARA-SCI categories correlated positively with parameters of aerobic fitness and muscular strength. Scores from the lifestyle activity PARA-SCI category were not consistently associated with fitness parameters. LTPA category scores could differentiate between groups differing by age, sex, gym or sports team membership, and frequency of participation in LTPA. Lifestyle and cumulative activity scores were unable to distinguish between most groups. CONCLUSION The convergent validity study provided evidence of validity for the PARA-SCI LTPA and cumulative activity categories. The extreme groups analyses provided further evidence of the validity of the LTPA category by demonstrating differences in extreme groups. Together, these findings contribute to the accumulating evidence of the construct validity of the PARA-SCI LTPA category and its utility for assessing LTPA among individuals with SCI. These results also highlight measurement constraints of the lifestyle activity and cumulative activity categories.
A Highly Accurate Bootstrapping Algorithm for Word Sense Disambiguation

In this paper, we present a bootstrapping algorithm for Word Sense Disambiguation which succeeds in disambiguating a subset of the words in the input text with very high precision. It uses WordNet and a semantic tagged corpus, for the purpose of identifying the correct sense of the words in a given text. The bootstrapping process initializes a set of ambiguous words with all the nouns and verbs in the text. It then applies various disambiguation procedures and builds a set of disambiguated words: new words are sense tagged based on their relation to the already disambiguated words, and then added to the set. This process allows us to identify, in the original text, a set of words which can be disambiguated with high precision; 55% of the verbs and nouns are disambiguated with an accuracy of 92%.
The role of competitive strategies on export market selection by SMEs in an emerging economy

The study examines the relationship between competitive strategies and export market selection by small and medium-sized exporters in an emerging economy. Drawing from the literature on comparative advantages of exporters from developing countries and demand characteristics in export markets, hypotheses are constructed on how cost leadership, differentiation and focus strategies are associated with exports to developed and developing countries. Survey data from 214 manufacturing SME exporters in Malaysia is used to empirically examine the relationships using ordinary least squares regression. The results suggest that cost strategies are positively associated with exports to developed countries but not developing countries. Differentiation strategies show the opposite effect, cost strategies and focus strategies are negatively associated with exports to both types of markets. This study contributes by demonstrating that competitive strategies act as a firm-level factor influencing export market selection. Implications and limitations of the study are presented.
Human Cytomegalovirus Infection of Langerhans-Type Dendritic Cells Does Not Require the Presence of the gH/gL/UL128-131A Complex and Is Blocked after Nuclear Deposition of Viral Genomes in Immature Cells

ABSTRACT Human cytomegalovirus (CMV) enters its host via the oral and genital mucosae. Langerhans-type dendritic cells (LC) are the most abundant innate immune cells at these sites, where they constitute a first line of defense against a variety of pathogens. We previously showed that immature LC (iLC) are remarkably resistant to CMV infection, while mature LC (mLC) are more permissive, particularly when exposed to clinical-strain-like strains of CMV, which display a pentameric complex consisting of the viral glycoproteins gH, gL, UL128, UL130, and UL131A on their envelope. This complex was recently shown to be required for the infection of immature monocyte-derived dendritic cells. We thus sought to establish if the presence of this complex is also necessary for virion penetration of LC and if defects in entry might be the source of iLC resistance to CMV. Here we report that the efficiency of LC infection is reduced, but not completely abolished, in the absence of the pentameric complex. While virion penetration and nuclear deposition of viral genomes are not impaired in iLC, the transcription of the viral immediate early genes UL122 and UL123 and of the delayed early gene UL50 is substantially lower than that in mLC. Together, these data show that the UL128, UL130, and UL131A proteins are dispensable for CMV entry into LC and that progression of the viral cycle in iLC is restricted at the step of viral gene expression.
High number of transplanted stem cells improves myocardial recovery after AMI in a porcine model

Abstract Objective. The clinical data considering the bone marrow mononuclear cell (BMMNC) therapy in treatment for acute myocardial infarction (AMI) are controversial and the mechanisms remain unknown. Our objective was to study the cardiac function and changes in cytokine levels after administration of BMMNC in experimental AMI model. Design. Unlabeled or Super-Paramagnetic-Iron-Oxide-labeled BMMNCs or saline was injected into myocardium of 31 pigs after circumflex artery occlusion. Ejection fraction (EF) was measured preoperatively, postoperatively and at 21 days by echocardiography. Cardiac MRI was performed postoperatively and after 21 days in 7 BMMNC animals. Serum cytokine levels were measured at baseline, 24 h and 21 days. Cellular homing was evaluated comparing MRI and histology. Results. From baseline to 21 days EF decreased less in BMMNC group (EF mean control -19 SD 12 vs. BMMNC -4 SD 15 percentage points p = 0.02). Cytokine concentrations showed high variability between the animals. MRI correlated with histology in cell detection and revealed BMMNCs in the infarction area. By MRI, EF improved 11 percentage points. The improvement in EF was associated with the number of transplanted BMMNCs detected in the myocardium. Conclusion. BMMNC injection after AMI improved cardiac function. Quantity of transplanted BMMNCs correlated with the improvement in cardiac function after AMI.
Cigarettes smoking habit may reduce benefit from cetuximab-based treatment in advanced colorectal cancer patients

Background: NF-κB is one of the nuclear effectors of EGFR activation. There are reports showing that NF-κB expression and activity is enhanced after nicotine treatment. Some data demonstrated that NF-κB activation plays a role in the induction of resistance to cetuximab and irinotecan in advanced colorectal tumors. The aim of this study was to evaluate the effect of cigarette smoking on cetuximab efficacy in advanced colorectal cancer patients. Methods: We retrospectively analysed the smoking habits of 200 patients treated with a variety of anticancer regimens containing cetuximab for advanced colorectal cancer. All patients were irinotecan-resistant and received an oxaliplatin-based first line treatment. We divided our patient population as follows: no previous smoking habit, previous smokers (any number of cigarettes), current smokers of less of 10 cigarettes/day, current smokers of more than 10 cigarettes/day. Results: Out of 200 patients 58 declared a history of cigarette smoking, 108 patients never smoked and the remaining 44 patients were cigarette smokers during cetuximab-based anticancer therapy. Of the 44 smokers, 18 smoked more than 10 cigarettes per day. No statistically significant differences in terms of response rate (RR) and time to progression (TTP) were identified between previous smokers and never smokers. RR in actual smokers was 13.6% and was lower than RR reported for non-smokers (27.1%; p = 0.023). In addition, the median TTP was 5.5 months in the non-smokers versus 2.8 months in the current smokers (p < 0.0001). A difference in terms of overall survival (OS) was detected between the two groups (p = 0.03). Comparing smokers of more than 10 cigarettes per day and smokers of less than 10 cigarettes per day no differences were detected in RR, TTP or OS. Conclusions: Our results suggest that cigarette smoking during anticancer treatment with a cetuximab-based regimen may be responsible for a decrease in RR and lead to a lower TTP.
Good governance for prison health in the 21st century. A policy brief on the organization of prison health.

The document reviews the reasons for the conclusion that prison health is public health, describes the legal cornerstones of prison health and the principle of equivalence and integration that should underlie it, lists the persistent shortcoming of current arrangements and spells out the meaning of good governance for prison health in the 21st century. The Expert group drew two conclusions about institutional arrangements for prison health: A¢â,¬Â¢managing and coordinating all relevant agencies and resources contributing to the health and well-being of prisoners is a whole-of-government responsibility; and A¢â,¬Â¢health ministries should provide and be accountable for health care services and advocate healthy conditions in prisons. Such governance would be in accordance with and supportive of the new European policy for health, Health 2020, and lead to better health and well-being for prisoners as part of better public health.
An approach to Lao-English rule based machine translation

This paper presents an approach to Lao-English rule based machine translation. In our approach, we start by modifying the Lao word segmented algorithm to mark the name entities part, which will be reordered according to its type later. Then, using the transfer-based strategy, the sentence is analyzed to build the source dependency structure, which is then transferred to the target dependency structure. The target text is then generated according to its attribute information. The machine translation performance obtained from the proposed method, in BLEU, METEOR, ROUGE-L, was 0.5920, 0.5482 and 0.8586, respectively. These results are better than those achieved by Google Translate.
Content-based digital signature for motion pictures authentication and content-fragile watermarking

The development of new multimedia services and environments requires new concepts both to support the new working process and to protect the multimedia data during the production and distribution. We address image/video authentication and copyright protection as major security demands in digital marketplaces. First we present a content-based signature technique for image and video authenticity and integrity. Based on this technique, we introduce a tool for interactive video authentication and propose content-fragile watermarking, a concept which combines watermarking and content-based digital signatures to ensure copyright protection and detection of integrity violation.
Pain relief in labour using transcutaneous electrical nerve stimulation (TENS). A TENS/TENS placebo controlled study in two parity groups

Summary. The analgesic effects of transcutaneous electrical nerve stimulation (TENS) in labour and effects on outcome were investigated in a double‐blind TENS/TENS placebo controlled trial in 100 primigravidae and 50 women in their third labour. There were no differences between the TENS and the TENS placebo users in terms of pain concept or relief, and only 12 and 13% of primigravidae and 48 and 39% of the para 2 women completed labour without requiring other analgesia in their respective groups. The primigravidae who used either TENS or TENS placebo alone had shorter labours than those who required further analgesia. Although the outcome of labour for mother and infant were similar in the two groups, there was a higher operative delivery rate in women who also had epidural analgesia. There were highly significant differences between the TENS and the TENS placebo users in terms of favourable and unfavourable comments by the mothers and the midwives at 1 and 24 h after delivery. The evident consumer satisfaction for TENS suggests TENS has a part to play in analgesia in labour but the equivocal findings in terms of factors associated with pain relief points to the need for apparatus more specifically designed to cope with the special characteristics of the pain of labour.
Acoustic Experience Alters the Aged Auditory System

Objectives: Presbyacusis, one of the most common ailments of the elderly, is often treated with hearing aids, which serve to reintroduce some or all of those sounds lost to peripheral hearing loss. However, little is known about the underlying changes to the ear and brain as a result of such experience with sound late in life. The present study attempts to model this process by rearing aged CBA mice in an augmented acoustic environment (AAE). Design: Aged (22–23 months) male (n = 12) and female (n = 9) CBA/CaJ mice were reared in either 6 weeks of low-level (70 dB SPL) broadband noise stimulation (AAE) or normal vivarium conditions. Changes as a function of the treatment were measured for behavior, auditory brainstem response thresholds, hair cell cochleograms, and gamma aminobutyric acid neurochemistry in the key central auditory structures of the inferior colliculus and primary auditory cortex. Results: The AAE-exposed group was associated with sex-specific changes in cochlear pathology, auditory brainstem response thresholds, and gamma aminobutyric acid neurochemistry. Males exhibited significantly better thresholds and reduced hair cell loss (relative to controls) whereas females exhibited the opposite effect. AAE was associated with increased glutamic acid decarboxylase (GAD67) levels in the inferior colliculus of both male and female mice. However, in primary auditory cortex AAE exposure was associated with increased GAD67 labeling in females and decreased GAD67 in males. Conclusions: These findings suggest that exposing aged mice to a low-level AAE alters both peripheral and central properties of the auditory system and these changes partially interact with sex or the degree of hearing loss before AAE. Although direct application of these findings to hearing aid use or auditory training in aged humans would be premature, the results do begin to provide direct evidence for the underlying changes that might be occurring as a result of hearing aid use late in life. These results suggest the aged brain retains significantly anatomical, electrophysiological, and neurochemical plasticity.
Derivation of induced pluripotent stem cells from pig somatic cells

For reasons that are unclear the production of embryonic stem cells from ungulates has proved elusive. Here, we describe induced pluripotent stem cells (iPSC) derived from porcine fetal fibroblasts by lentiviral transduction of 4 human (h) genes, hOCT4, hSOX2, hKLF4, and hc-MYC, the combination commonly used to create iPSC in mouse and human. Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) and in medium supplemented with knockout serum replacement and FGF2. Compact colonies of alkaline phosphatase-positive cells emerged after ≈22 days, providing an overall reprogramming efficiency of ≈0.1%. The cells expressed porcine OCT4, NANOG, and SOX2 and had high telomerase activity, but also continued to express the 4 human transgenes. Unlike human ESC, the porcine iPSC (piPSC) were positive for SSEA-1, but negative for SSEA-3 and -4. Transcriptional profiling on Affymetrix (porcine) microarrays and real time RT-PCR supported the conclusion that reprogramming to pluripotency was complete. One cell line, ID6, had a normal karyotype, a cell doubling time of ≈17 h, and has been maintained through >220 doublings. The ID6 line formed embryoid bodies, expressing genes representing all 3 germ layers when cultured under differentiating conditions, and teratomas containing tissues of ectoderm, mesoderm, and endoderm origin in nude mice. We conclude that porcine somatic cells can be reprogrammed to form piPSC. Such cell lines derived from individual animals could provide a means for testing the safety and efficacy of stem cell-derived tissue grafts when returned to the same pigs at a later age.
UvA-DARE ( Digital Academic Repository ) Price dynamics in equilibrium models

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A comparison of actigraphy and polysomnography in older adults treated for chronic primary insomnia.

STUDY OBJECTIVES The present study explores the accuracy and clinical utility of actigraphy compared with polysomnography in older adults treated for chronic primary insomnia. DESIGN Polysomnographic and actigraphic data were collected before and after treatment. SETTING A university-based outpatient clinic for adults and elderly. PARTICIPANTS Thirty-four participants with chronic primary insomnia. Mean age was 60.5 years. INTERVENTIONS Participants received either a manualized treatment package based on cognitive-behavior therapy and sleep management or hypnotic-drug treatment (7.5 mg zopiclone) for 6 weeks (these findings are reported elsewhere). MEASUREMENTS AND RESULTS Although the sensitivity of actigraphy to detect sleep was very high (95.2%), actigraphy performed poorly in detecting wakefulness (specificity: 36.3%), yielding on an overall level of accuracy of 83.1%. However, the level of actigraphy accuracy was dependent upon polysomnography-registered sleep efficiency. Actigraphy underestimated total wake time and sleep-onset latency and consequently overestimated total sleep time and sleep efficiency. Compared with polysomnography, actigraphy captured only part of the treatment effects on total wake time and sleep-onset latency and failed to detect significant changes in sleep efficiency. CONCLUSIONS The present findings suggest that the clinical utility of actigraphy is still suboptimal in older adults treated for chronic primary insomnia and should, hence, be used in this clinical setting with the concurrent use of supplementary assessment methods.