Miracle-dz/UniFAR-FTUs huggingface dataset:
Human dolichol‐phosphate‐mannose synthase consists of three subunits, DPM1, DPM2 and DPM3

Dolichol‐phosphate‐mannose (DPM) synthase generates mannosyl donors for glycosylphosphatidylinositols, N‐glycan and protein O‐ and C‐mannosylation. In Saccharomyces cerevisiae, this enzyme is encoded by DPM1. We reported previously that mammalian DPM synthase contains catalytic DPM1 and regulatory DPM2 subunits, and that DPM1 requires DPM2 for its stable expression in the endoplasmic reticulum. Here we report that human DPM synthase consists of three subunits. The third subunit, DPM3, comprises 92 amino acids associated with DPM1 via its C‐terminal domain and with DPM2 via its N‐terminal portion. The stability of DPM3 was dependent upon DPM2. However, overexpression of DPM3 in Lec15 cells, a null mutant of DPM2, restored the biosynthesis of DPM with an increase in DPM1, indicating that DPM3 directly stabilized DPM1. Therefore, DPM2 stabilizes DPM3 and DPM3 stabilizes DPM1. DPM synthase activity was 10 times higher in the presence of DPM2, indicating that DPM2 also plays a role in the enzymatic reaction. Schizosaccharomyces pombe has proteins that resemble three human subunits; S.pombe DPM3 restored biosynthesis of DPM in Lec15 cells, indicating its orthologous relationship to human DPM3.
Micronutrient content does not reflect the status of health in the aquatic carnivorous plant Aldrovanda vesiculosa

Tissue micronutrient content (B, Fe, Mn, Zn, Cu, Mo, Co) was compared in healthy and ill shoots of a rootless aquatic carnivorous plant Aldrovanda vesiculosa growing in an outdoor culture, in plants in the field, in different accessions from around the world, and in shoots and turions. On the basis of microelemental analyses, the observed disorder of shoot apices cannot be explained as deficiency of any of the above micronutrients.
Imaging procedures for the inverse synthetic aperture radar application

The paper discusses the approaches used in the algorithm design of radar image processing. They are based on parametric and non-parametric spectral analysis and compared to each other using well-known example of simulated data for the inverse synthetic aperture radar application. The goal of the processing is meeting two requirements which are reducing smeared Doppler shifts and improving image resolution. The proposed parametric identification method allows estimating signal model parameters for the short dwell time and extrapolating radar data outside this time. This radar image performance satisfies the requirements as well as joint time-frequency transform.
Dropout training for Hidden Unit CRFs

A very commonly faced issue while training prediction models using machine learning is overfitting. Dropout is a recently developed technique designed to counter this issue in deep neural networks and has also been extended to other algorithms like SVMs. In this project, we formulate and study the application of Dropout to Hidden Unit Conditional Random Fields (HUCRFs). HUCRFs use binary stochastic hidden units to model the underlying latent structure in the data and hence are similar to Neural Networks. The Dropout technique proposed in this report involves dropping of a random set of hidden states for each training example. For test inference, we formulate an inference equation that is equivalent to computing expected prediction using the ensemble of all Dropout models. In this report, we also evaluate our Dropout train and test formulations when used for OCR. We evaluate the models by varying complexity of the models and the amount of dropout. Results show that HUCRF models trained with dropout consistently perform better than baseline HUCRF.
Changes in Acoustic and Kinematic Articulatory Working Space Across Three Intensity Levels

Changes in Acoustic and Kinematic Articulatory Working Space Across Three Intensity Levels Panika Ellis Palmer Department of Communication Disorders, BYU Master of Science The purpose of this study was to compare changes in acoustic and kinematic measures of articulation across soft, comfortable, and loud speech conditions. There were 19 participants, 9 male and 10 female, with age ranging from 20 to 34 with a median age of 25. Each participant had electromagnetic sensors glued to their tongue, jaw, and lips. It was anticipated that the acoustic measures would accurately reflect the kinematic measures of speech as articulation changed across the intensity levels. Vowel space area (VSA) and vowel articulation index (VAI) were computed from the three corner vowels, /ɑ, i, u/. Articulatory-acoustic vowel space (AAVS), a sentence-level acoustic measure, was computed from the continuous formant histories for all voiced segments in a sentence. Kinematic-vowel space area (KVSA), kinematicvowel articulation index (KVAI), and articulatory-kinematic vowel space (AKVS) were the kinematic equivalents of the acoustic measures, and were newly developed for the present study. Stroke metrics based on the speed history of the lingual movements were also used to reveal average kinematic features of the articulatory gestures in each participant’s speech. The data revealed that the isolated acoustic and kinematic measures that used corner vowels (VSA, VAI. KVSA, KVAI) did not change significantly with intensity. The sentence-level continuous measures of articulatory working space (AAVS and AKVS) increased as speech intensity increased. The other sentence-level kinematic metrics also changed significantly with speech intensity, including increases in hull volume, onset speed, peak speed, mean speed, and distance. Stroke duration decreased as speech intensity increased. These findings suggest that measures based on isolated corner vowels are not as reflective as continuous measures of changes in articulatory movement in speech.
Identification of a Novel Conserved Motif in the STAT Family That Is Required for Tyrosine Phosphorylation*

The rapid transcriptional activation of cellular genes by either type 1 interferons (IFNα/β) or type 2 interferon (IFNγ) is responsible for many of the pleiotropic effects of these cytokines, including their antiviral, antigrowth, and immunomodulatory activities. Interferon-stimulated gene expression is mediated by transcription factors termed Stats, which upon being tyrosine-phosphorylated, translocate to the nucleus and bind enhancers of interferon-activated genes. We have recently characterized a new Jurkat cell variant, named H123, where IFNα stimulates programmed cell death. H123 clones that are resistant to the apoptotic actions of IFNα have been selected. One of these clones (Clone 8) is defective in its responses to IFNα with regard to activation of genes that require tyrosine phosphorylation of Stat2. Stimulation of Clone 8 cells with IFNα induces normal tyrosine phosphorylation of Stat1 and Stat3. Sequencing of Stat2 RNA reveals a substitution of proline 630 located within the Src homology 2 domain of Stat2 to leucine (P630L). Pro-630 and its adjacent amino acids are conserved in all Stat family members but are absent in other proteins that contain Src homology 2 domains. Expression of Stat2 P630L in cells inhibits IFNα-stimulated gene expression. These results not only define a critical motif in Stat2 required for its transcriptional activity, but they also provide evidence that resistance to type one IFNs can be mediated by mutations in Stat2 as well as those previously described for Stat1.
Palynological study of the genus Linum in Iran (a taxonomic review)

Linum is the main genus of the Flax family widely distributed across the word with about 230 species. In the present study, pollen grains of 15 taxa from four sections of this genus were examined with scanning electron microscope and light microscope. The taxa primarily differed in polar and equatorial shape and their axis length, patterns of exine sculpturing and types of surface fine structure. Pollen shapes in the equatorial and polar view were circular, angular or elliptic. The applied ANOVA test for palynological characters showed a significant difference (p<0.001) of some quantitative characters. Sculpture characters on the basis of their form, size, number, distribution, and fine structure showed variations at different taxonomic levels and were useful in the identification of species, subspecies and varieties in the genus. A significant positive/negative correlation was seen between some characters in the pollen grains. Palynological data, however, cannot show the species relationship in the sections; they are of taxonomic importance and may be used for differentiation of the species, subspecies and varieties.
Synthesis, DNA-binding, Photocleavage and in vitro Cytotoxicity of NovelImidazole[4,5-f][1,10]phenanthroline-based Oxovanadium Complexes

Three novel imidazole[4,5-f][1,10]phenanthroline-based oxovanadium complexes [VO(hntdtsc)(HPIP)] (1) (hntdtsc = 2-hydroxyl-1-naphthaldehyde thiosemicarbazone, HPIP = 2-(2-hydroxylphenyl)imidazole[4,5-f][1,10]phenanthroline), [VO(hntdtsc)(m-HPIP)] (2) (m-HPIP = 2-(3-hydroxylphenyl)imidazole[4,5-f][1,10]phenanthroline), [VO(hntdtsc)(p-HPIP) (3) (p-HPIP = 2-(4-hydroxylphenyl)imidazole[4,5-f][1,10]phenanthroline), have been synthesized and characterized by elemental analyses and spectroscopic techniques. Their DNA-binding properties with calf-thymus DNA (CT-DNA) were studied by various methods. The cytotoxicity of these three complexes was evaluated by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The electronic spectral results reveal that three complexes can bind to CT-DNA by intercalation mode. The electrophoresis studies also show that they can efficiently cleave pBR322 DNA. The in vitro antiproliferative activity of complex 1 against human CaSki, SiHa, K562, HepG2, EC9706 and EC109 cell lines is proved to be more effective than both 2 and 3.
The value of capture: Taking an alternative approach to using lecture capture technologies for increased impact on student learning and engagement

Lecture Capture technologies are becoming widespread in UK Higher Education with many institutions adopting a capture-all approach. Installations of capture devices in all teaching rooms and lecture theatres, scheduled recordings through integration with timetabling and automated distribution through virtual learning environments are swiftly becoming the norm. Capturing lectures has been shown to have a positive impact on student satisfaction, but numerous studies have shown little or no positive impact on student attainment as a result of capturing lectures. This article explores an alternative approach to the use of capture technologies in a pilot study at the University of Wolverhampton. The output of the pilot evaluation is a theoretical model recommending a shift in focus away from the conventional use of the technology for capturing lectures. It advocates a move toward the purposeful use of capture technologies to create content which adds value to student learning and increases engagement, which may ultimately lead to a positive impact on student attainment. The findings have implications for policy and practice around the use of capture technologies. Future work is described in the context of the project findings. [ABSTRACT FROM AUTHOR]
P6C-10 Evaluation of the Structure Factor Size Estimator (SFSE) with Simulated Ultrasonic Backscattered Signals from Blood

Ultrasonic backscattered signals from blood contain frequency-dependent information that can be used to obtain quantitative parameters reflecting the aggregation state of red blood cells (RBCs). Recently, the structure factor size estimator (SFSE) was developed to solve for two parameters: the packing factor W and mean gyration radius of aggregates Rg, expressed in number of RBCs (Yu and Cloutier 2007, J. Acoust. Soc. Am. 122 645-656). Herein, the SFSE is assessed by computer simulations where deterministic rheology parameters, such as the hematocrit, number of RBCs per aggregate, and mean gyration radius of aggregates, are known. RBC aggregation was simulated at 40% hematocrit and mean gyration radii of clusters ranged from 1.6 (quasi-disaggregated RBCs) to 6.4. US frequencies were varied between 20 and 42 MHz, and the insoniflcation angle was fixed to 90deg (perpendicular to the flow). For the quasi-disaggregated RBCs, the packing factor W evaluated with the SFSE from the simulated signals matched those expected from theory: W was similar to the Perkus Yevick packing factor. For aggregating RBCs, good correlation was obtained between the gyration radii evaluated with the SFSE model and those expected (r2 = 0.96). To conclude, this study shows the SFSE ability to estimate blood backscattering properties at a physiological hematocrit of 40% and open the way to parametric imaging (using the SFSE) in abnormal blood conditions promoting thrombosis.
Low-complexity CFO correction of frequency-spreading SMT in uplink of multicarrier multiple access networks

This paper presents an alternative method to compensate carrier frequency offset (CFO) for frequency-spreading structure of staggered modulated multitone (FS-SMT) system in the uplink of multiple access scenario. The proposed method reduces computational complexity and latency of the receiver without losing the performance. Moreover, a possibility for merging compensation of timing offset (TO) and channel effect with CFO correction is presented. Simulation results show that the proposed method offers a reasonable performance with mentioned improvements.
FR901228 causes mitotic arrest but does not alter microtubule polymerization

FR901228, a natural cyclic depsipeptide, shows high cytotoxicity against human cancer cell lines (low nM IC50 values). Cells exposed to FR901228 arrest with G1 or G2/M DNA content; S phase is depleted. G2/M cells include cells arrested in mitosis. We wished to understand the mitotic arrest by this compound. Mitotic arrest is often due to interference with microtubules and COMPARE testing in the NCI drug screen indicated a possible taxane-like mechanism. Testing of FR901228 for tubulin binding or alteration of in vitro MT assembly failed to reveal any effect. Likewise, examination of cellular microtubules following exposure to FR901228 did not reveal any change. Similar G2/M accumulation was observed in MCF7, MCF10 and PC3 cells. About 50% of G2/M cells were mitotic and contained microtubule spindles. Mitotic cells peaked at about 14-16 h drug exposure and declined to near 0% by 24-30 h. The block was at prometaphase, with numerous chromosomes unattached to the spindle. We conclude that FR901228 induces formation of aberrant spindles probably by interfering with chromosome attachment, causing mitotic accumulation without affecting mitotic microtubules.
Multiple-access channels with different generalized feedback signals

This paper is concerned with a communication channel with two senders and one receiver, in which each sender observes a private feedback signal. The two feedback signals are not necessarily equivalent to or derived from the signal observed by the receiver. An achievable rate region is demonstrated for this multiple-access channel by means of a new superposition coding scheme. In particular it is shown that a rate region, previously obtained for the multiple-access channel with "perfect" feedback to both senders, remains achievable when the feedback connection to one of the senders is eliminated.
Characterization of Ubiquitin/Proteasome-Dependent Regulation of Hap2/3/4/5 Complex In Saccharomyces cerevisiae

v Introduction . 1 Saccharomyces cerevisiae as a model system . 1 The Metabolism of a Facultative Aerobe . 2 The Hap2/3/4/5 Complex . 8 Mitochondrial Biogenesis . 13 The Ubiquitin-Protease System . 14 Materials and Methods . 18 Results . 23 Preliminary Findings from Liu Lab . 23 Goals of Research . 23 Research Results . 24 Discussion . 33 Conclusion . 38 Bibliography . 39
An improved interactive genetic algorithm incorporating relevant feedback

This paper has proposed a new interactive genetic algorithm (IGA) framework incorporating relevant feedback (RF), in which human evaluation is regarded as not only the fitness function of GA, but also the relevant score to instruct interactive machine learning. Thus, on the one hand, user's fatigue, the key issue of IGA, can be alleviated, since some individuals with higher preference weight are added in each generation through relevance feedback technology. On the other hand, the two mapping functions between the low-level parameter space and the high-level users' psychological space can be built during interactions. An instance of this frame, which uses support vector machine (SVM) as the machine learning method in RF, is also provided. The effectiveness of our approach is first evaluated through simulation tests using two benchmark functions. The experimental results show that the convergence speed of the proposal is much faster than that of normal IGA. Then, the approach is applied to retrieve images with emotion semantics queries. The subject experiments also demonstrate that the proposal algorithm can alleviate user fatigue. Furthermore, SVM constructs an individual emotion user model though learning.
Whole-body vibration during manual wheelchair propulsion with selected seat cushions and back supports

Although the exposure to whole-body vibrations (WBV) has been shown to be detrimental to seated humans, the effects of wheelchairs and seating systems on the transmission of vibration to an individual have not been thoroughly examined. The purpose of this study was to determine if the selected wheelchair seat cushions and back supports minimize the transmission of vibrations. Thirty-two wheelchair users traversed an activities of daily living course three times using 16 randomly selected seating systems as well as their own. Vibrations were measured using triaxial accelerometers at the seat and participant's head. The weighted fore-to-aft (T/sub x/), vertical (T/sub z/), and resultant (T/sub r/) transmissibility based on the vibrational-dose-value (VDV) were used to determine if differences existed among the four seat cushions and back supports. The obstacles that seem to have the largest effect on the transmission of WBV are the single event shocks and the repeated event shocks. Comparisons between the individuals own seating system and the tested seating systems suggest that the individuals are not using the most appropriate seating system in terms of the reduction of vibration transmission.
Histopathological changes in the liver of rabbits exposed to high nitrate ingestion in drinking water.

OBJECTIVES In India, especially in Rajasthan, people drink water which contains high level of nitrates and the possibility of finding concentrations of up to 500 mg of nitrate ions per litre of water is not unusual. Excessive use of nitrate fertilisers and herbicides results in accumulation of nitrate in plants and methemoglobinaemia in cattle as consequences of nitrate poisoning. The ingested nitrate is converted to nitrite in the digestive system and it is absorbed in blood, thus causing methemoglobinaemia. Methaemoglobinaemia is not restricted to infants alone, but it is prevalent in higher age groups also. METHODS Therefore, an experimental study was conducted on 10 rabbits which were between three and a half months to four months of age, which had weights which ranged from 1.310 kg to 1.720 kg. Five groups A, B, C,D and E were formed, with two rabbits in each group. The control Group A was given water orally, which had 45 mg/litres of nitrate. Groups B to E (experimental groups) were administered water orally, which had concentrations of 100mg/litre, 200mg/litre, 400mg/litre and 500mg/litre of nitrate respectively, for 120 days. During experimental period, the differences in general behaviour of rabbits were noted. After this, rabbits were anaesthetised and sacrificed according to guidelines of ICMR and their livers were removed and processed for making paraffin sections,. Hematoxyllin and eosin staining was done for microscopic observations. RESULTS During experimental period, the animals were found to be lethargic on 75(th) day. Quantity of intake of food and water was not altered in the rabbits which were undergoing experiments in different groups. Rabbits of all groups i.e. A to E showed a continuous increase in heart rate (up to 218/minute in Group E) and respiration rate (up to 84/minute in Group E) respectively. The microscopic study showed mild necrosis of hepatocytes, with infiltration of inflammatory cells in between hepatocytes. In higher groups, the liver showed bridging necrosis and portal triditis. Dilatations of central vein with eosinophilic degeneration were observed in Group E only.
Integrated Planning for School and Community: The Case of Johnston County, North Carolina

OR/Ed Laboratories and the Johnston County schools created a planning system, Integrated Planning for School and Community, to forecast enrollments, to compare the enrollment projections to capacity, to find the optimal locations for new school buildings, and to set distance-minimized boundaries forall schools to avoid over crowding and to meet racial balance guidelines. Implementing the system has increased the school district's success in passing bond issues, reduced pupil transportation costs, and eliminated frequent adjustments to school-attendance boundaries.
Abnormal Sections Detection Based on Inter-Frames Information in Video Sequence

An approach is proposed for abnormal sections detection and rank classification in video sequences. In this approach, we give the definition of the abnormality of the video sections and create an abnormal factor to evaluate the abnormality. Firstly, the histogram is selected to describe the color change in the section, and then the histograms of the frames selected from the section compose the histogram matrix. In order to improve the process efficiency, the principal components analysis is used to reduce dimensions of the histogram matrix. Secondly, the information of frames differences is used to describe the motion change information, and the information twice frame difference is used to describe the change rate. Lastly, the results from the features are fused to get the final result. This method is experimented with some samples. The results show that this method can realize the abnormal detection and rank classification of video sequences, and the results are better than the other related techniques.
Normothermic Ex Vivo Perfusion Prevents Lung Injury Compared to Extended Cold Preservation for Transplantation

Treatment of injured donor lungs ex vivo to accelerate organ recovery and ameliorate reperfusion injury could have a major impact in lung transplantation. We have recently demonstrated a feasible technique for prolonged (12 h) normothermic ex vivo lung perfusion (EVLP). This study was performed to examine the impact of prolonged EVLP on ischemic injury. Pig donor lungs were cold preserved in Perfadex® for 12 h and subsequently divided into two groups: cold static preservation (CSP) or EVLP at 37°C with Steen™ solution for a further 12 h (total 24 h preservation). Lungs were then transplanted and reperfused for 4 h. EVLP preservation resulted in significantly better lung oxygenation (PaO2 531 ± 43 vs. 244 ± 49 mmHg, p < 0.01) and lower edema formation rates after transplantation. Alveolar epithelial cell tight junction integrity, evaluated by zona occludens‐1 protein staining, was disrupted in the cell membranes after prolonged CSP but not after EVLP. The maintenance of integrity of barrier function during EVLP translates into significant attenuation of reperfusion injury and improved graft performance after transplantation. Integrity of functional metabolic pathways during normothermic perfusion was confirmed by effective gene transfer and GFP protein synthesis by lung alveolar cells. In conclusion, EVLP prevents ongoing injury associated with prolonged ischemia and accelerates lung recovery.