Miracle-dz/UniFAR-FTUs huggingface dataset:
Injection-Locked CMOS Frequency Doublers for $\mu$-Wave and mm-Wave Applications

On-chip frequency generators for high frequency applications suffer from degradation of key passive components, variable capacitors in particular. In this framework, frequency multipliers can play a key role, allowing the design of voltage-controlled oscillators running at a frequency lower than required with advantage in terms of signal spectral purity and frequency tuning range. In this paper we present two injection locked frequency doublers for Ku-band and F-band applications respectively. Despite differences in implementation details, the same topology where a push-push pair injects a double frequency tone locking an autonomous differential oscillator is adopted. The circuits require limited input signal swing and provide a differential output over a broad frequency range. Dissipating 5.2 mW, the Ku-band multiplier, realized in a 0.13 μm CMOS node, displays an operation bandwidth from 11 GHz to 15 GHz with a peak voltage swing on each output of 470 mV. The F-band multiplier, realized in 65 nm CMOS technology, displays an operation bandwidth from 106 GHz to 128 GHz with a peak voltage swing on each output of 330 mV and a power dissipation of 6 mW. A prototype including the multiplier, driven by a half-frequency standard LC-tank VCO, demonstrates an outstanding 13.1% tuning range around 115 GHz.
Clathrin-dependent Endocytosis Is Required for TrkB-dependent Akt-mediated Neuronal Protection and Dendritic Growth*

Endocytosis of Trk (tropomyosin-related kinase) receptors is critical for neurotrophin signal transduction and biological functions. However, the mechanism governing endocytosis of TrkB (tropomyosin-related kinase B) and the specific contributions of TrkB endocytosis to downstream signaling are unknown. In this study, we report that blocking clathrin, dynamin, or AP2 in cultured neurons of the central nervous system inhibited brain-derived neurotrophic factor (BDNF)-induced activation of Akt but not ERK. Treating neurons with the clathrin inhibitor monodansylcadaverine or a peptide that blocks dynamin function specifically abrogated Akt pathway activation in response to BDNF but did not affect the response of other downstream effectors or the up-regulation of immediate early genes neuropeptide Y and activity-regulated cytoskeleton-associated protein. Similar effects were found in neurons expressing small interfering RNA to silence AP2 or a dominant negative form of dynamin that inhibits clathrin-mediated endocytosis. In PC12 cells, ERK but not Akt activation required TrkA endocytosis following stimulation with nerve growth factor, whereas the opposite was true when TrkA-expressing neurons were stimulated with nerve growth factor in the central nervous system. Thus, the specific effects of internalized Trk receptors probably depend on the presence of cell type-specific modulators of neurotrophin signaling and not on differences inherent to Trk receptors themselves. Endocytosis-dependent activation of Akt in neurons was found to be critical for BDNF-supported survival and dendrite outgrowth. Together, these results demonstrate the functional requirement of clathrin- and dynamin-dependent endocytosis in generating the full intracellular response of neurons to BDNF in the central nervous system.
Lentivirally generated eGFP‐transgenic rats allow efficient cell tracking in vivo

Here we describe the efficient generation of eGFP‐transgenic rats using a lentiviral approach. Analysis of the founder generation demonstrated that 46% of the offspring had stably integrated the provirus into the genome and of those 92% expressed eGFP in all blood‐derived leukocytes. In contrast to their offspring, all founder rats were mosaic with regard to eGFP‐expression, suggesting delayed viral transduction after injection. The expression level of eGFP in the F1 generation is influenced by and segregates with the site of proviral integration. Interestingly, a single copy of the transgene is sufficient for reliable detection by flow cytometry, irrespective of the leukocyte subtype analyzed. Adoptive transfer of purified CD4+ T‐lymphocytes from transgenic rats and subsequent reisolation from various organs further demonstrated that expression of the lentiviral transgene is maintained in a foreign host and therefore allows for efficient tracking of transferred cells. Taken together, lentivirally generated eGFP‐transgenic rats are a powerful tool for various applications in immunology and presumably also many other fields. genesis 39:94–99, 2004. © 2004 Wiley‐Liss, Inc.
Protective effect of chokeberry on chemical-induced oxidative stress in rat

Male Wistar rats were treated with chokeberry juice per os, 10 mL/kg/day, for 28 days and a single intraperitoneal (i.p.) dose of N-nitrosodiethylamine (NDEA), 150 mg/kg, or carbon tetrachloride (CCl4), 2 ml/kg. The level of hepatic microsomal lipid peroxidation, expressed as thiobarbituric acid reactive substances (TBARS), was increased in animals dosed with NDEA and CCl4. Juice pretreatment resulted in a significant decrease in TBARS by 53% and 92%, respectively. In rats administered juice alone, 50% decrease in TBARS was noted. The activities of all antioxidant enzymes were decreased in the liver of rats administered either toxicant by 29%—52% as compared to controls. Juice pretreatment resulted in an increase in the activity of catalase, glutathione peroxidase and glutathione reductase by 117%, 56% and 44%, respectively, only in rats challenged with NDEA. Although no response of plasma protein carbonyls to both toxicants was observed, the pretreatment with juice caused a 55% decrease of this parameter in CCl4—dosed rats. DNA damage in blood leukocytes induced by either toxicant was slightly reduced, by 24%, in the rats pretreated with juice and administered NDEA. The results of the study showed that pretreatment with chokeberry juice confers some protection against chemical-induced oxidative stress.
Ultrastructure and Molecular Phylogeny of Mesodinium coatsi sp. nov., a Benthic Marine Ciliate

Mesodinium is a globally distributed ciliate genus forming frequent and recurrent blooms in diverse marine habitats. Here, we describe a new marine species, Mesodinium coatsi n. sp. , originally isolated from interstitial water of surface sand samples collected at Mohang Beach, Korea. The species was maintained under a mixotrophic growth condition for longer than 1 yr by providing a cryptomonad, Chroomonas sp. , as the sole prey. Cell morphology and subcellular structure were examined by light microscopy, scanning, and transmission electron microscopy, and molecular phylogeny was inferred from nuclear‐encoded 18S rDNA sequence data. Like other Mesodinium species, M. coatsi consisted of two hemispheres separated by two types of kinetids, and had tentacles located at the oral end of the cell. Several food vacuoles were observed in the cytoplasm, and partially digested prey cells sometimes existed in food vacuoles. Kinetids and the associated accessory structures were quite similar to those previously reported, but M. coatsi was differentiated from other marine Mesodinium species by ultrastructural characters of the dikinetids, polykinetids, and tentacles. We also provided a detailed illustration of infraciliature. Molecular phylogeny revealed that M. coatsi and Mesodinium chamaeleon were closely related to each other.
The Adapter Protein Apoptotic Protease-activating Factor-1 (Apaf-1) Is Proteolytically Processed during Apoptosis*

Apoptotic protease-activating factor-1 (Apaf-1), a key regulator of the mitochondrial apoptosis pathway, consists of three functional regions: (i) an N-terminal caspase recruitment domain (CARD) that can bind to procaspase-9, (ii) a CED-4-like region enabling self-oligomerization, and (iii) a regulatory C terminus with WD-40 repeats masking the CARD and CED-4 region. During apoptosis, cytochromec and dATP can relieve the inhibitory action of the WD-40 repeats and thus enable the oligomerization of Apaf-1 and the subsequent recruitment and activation of procaspase-9. Here, we report that different apoptotic stimuli induced the caspase-mediated cleavage of Apaf-1 into an 84-kDa fragment. The same Apaf-1 fragment was obtained in vitro by incubation of cell lysates with either cytochrome c/dATP or caspase-3 but not with caspase-6 or caspase-8. Apaf-1 was cleaved at the N terminus, leading to the removal of its CARD H1 helix. An additional cleavage site was located within the WD-40 repeats and enabled the oligomerization of p84 into a ∼440-kDa Apaf-1 multimer even in the absence of cytochromec. Due to the partial loss of its CARD, the p84 multimer was devoid of caspase-9 or other caspase activity. Thus, our data indicate that Apaf-1 cleavage causes the release of caspases from the apoptosome in the course of apoptosis.
Cryptic diversity in European bats

Different species of bat can be morphologically very similar. In order to estimate the amount of cryptic diversity among European bats we screened the intra– and interspecific genetic variation in 26 European vespertilionid bat species. We sequenced the DNA of subunit 1 of the mitochondrial protein NADH dehydrogenase (ND1) from several individuals of a species, which were sampled in a variety of geographical regions. A phylogeny based on the mitochondrial (mt) DNA data is in good agreement with the current classification in the family. Highly divergent mitochondrial lineages were found in two taxa, which differed in at least 11% of their ND1 sequence. The two mtDNA lineages in Plecotus austriacus correlated with the two subspecies Plecotus austriacus austriacus and Plecotus austriacus kolombatovici. The two mtDNA lineages in Myotis mystacinus were partitioned among two morphotypes. The evidence for two new bat species within Europe is discussed. Convergent adaptive evolution might have contributed to the morphological similarity among distantly related species if they occupy similar ecological niches. Closely related species may differ in their ecology but not necessarily in their morphology. On the other hand, two morphologically clearly different species (Eptesicus serotinus and Eptesicus nilssonii) were found to be genetically very similar. Neither morphological nor mitochondrial DNA sequence analysis alone can be guaranteed to identify species.
The association between internet addiction and self-injurious behaviour among adolescents

Objective: To examine the association between internet addiction and self-injurious behaviour (SIB) in adolescence. Methods: Population-based cross-sectional survey of 1618 high school students aged 13–18 years in Guangzhou city, Guangdong Province, PR China. Deliberate SIB was measured using self-reported questionnaire; internet addiction was assessed using the Internet Addiction Test (IAT). Results: 263 (16.3%) participants reported having committed some form of SIB in the past 6 months. 73 (4.5%) had committed SIB 6 times or more, and 157 (9.7%) 1–5 times. The majority of respondents were classified as normal users of the internet (n = 1392, 89.2%), with 158 (10.2%) moderately and 10 (0.6%) severely addicted to the internet. After adjusting for potential confounders, the odds ratio for SIB was 2.0 (95% CI 1.1 to 3.7) for those who were classified as moderately and severely addicted to the internet when compared to the normal group. Conclusions: SIB is common in adolescence in the study population in China. Addiction to the internet is detrimental to mental health and increases the risk of self-injury among adolescents. Clinicians need to be aware of potential co-morbidities of other addictions among adolescent self-injured patients.
Adaptation to abrupt time shifts of the oscillator(s) controlling human circadian rhythms.

1. Thirty‐six subjects in an isolation unit were subjected to time shifts of 12 hr, or of 8 hr in either direction. 2. The rhythms of body temperature and excretion of eight urinary constituents were studied before and after the shift, both on a usual nychthemeral routine and during 24 hr when they remained under constant conditions, awake, engaged in light, mainly sedentary activity, and consuming identical food and fluid every hour. 3. The rhythms on nychthemeral routine were defined by fitting cosine curves. On constant routine the rhythm after the shift was cross‐correlated with the original rhythm, either with variable delay (or advance) or with an additive mixture between this variably shifted rhythm and the unshifted or a fully shifted rhythm. The process yielding the highest correlation coefficient was accepted as the best descriptor of the nature of adaptation. 4. A combination of two rhythms was observed more often for urinary sodium, chloride and phosphate than for other variables. 5. Adaptation appeared to have proceeded further after westward than eastward shifts, and this difference was particularly noticeable for urinary potassium, sodium and chloride. 6. Partial adaptation usually involved a phase delay, even after an eastward shift when a cumulative delay of 16 hr would be needed to achieve full adaptation and re‐entrainment. 7. Observations under nychthemeral conditions often gave a false idea of the degree of adaptation. In particular, after an eastward shift the phase of the rhythms appeared to shift in the appropriate direction when studied under nychthemeral conditions whereas the endogenous oscillator either showed no consistent behaviour or, in the control of urate excretion, a shift in the wrong direction. 8. The implications for people undergoing time shifts, in the course of shift work or transmeridional flights, are indicated.
Digital PID based load frequency control through open communication networks

In this paper, a delay-dependent method is adopted to deal with the continuous-discrete operation mode and time delays induced by communication through open networks in load frequency control (LFC) scheme. By using a novel technique, the design problem of digital PID-type controller is converted into a design problem of static output feedback controller based on the discrete-time system model. The controller gains are obtained using networked control system theory, linear matrix inequality technique and cone complementarity linearization algorithm. By taking the developed systematic procedures, digital PID-type controllers for LFC scheme of a three-area interconnected power system are designed. Simulation results show that the designed controllers provide better robustness against certain time delays than the existing controllers.
Progress and Proficiency: Redesigning Grading for Competency Education. CompetencyWorks Issue Brief.

Acknowledgments This paper could not possibly have happened without the insights, wisdom, and nudges from Rose Colby. As always, Susan Patrick has been a collaborative and incredibly supportive partner in advancing competency education. I would also like to thank the many people who dedicated time to share their experiences, including Also, I want to thank all the educators who hosted me during school visits. Many of the insights included in this paper were gained during quick conversations in classrooms and hallways with principals, teachers, and students. CompetencyWorks is a collaborative initiative drawing on the knowledge of its partners and advisory board. The International Association for K–12 Online Learning (iNACOL) is the lead organization with project management facilitated by MetisNet. We are deeply grateful for the leadership and support from the partner organizations American Youth Policy Forum, Jobs for the Future, and the National Governors Association. Their vision and creative partnership have been instrumental in the development of CompetencyWorks.
Computerized linguistic resources of the research laboratory ATILF for lexical and textual analysis: Frantext, TLFi, and the software Stella

This paper presents some of the computerized linguistic resources of the Research Laboratory ATILF (Analyse et Traitement Informatique de la Langue Francaise) available via the Web, and will serve as a helping document for demonstrations planned within the framework of LREC 2002. The Research Laboratory ATILF is the new UMR (Unite Mixte de Recherche) created in association between the CNRS and the University of Nancy 2 since 2001-January 2nd, and succeeds to the local component of the INaLF situated in Nancy. This considerable amount of resources concerning French language consists in a set of more than 3400 literary works grouped together in Frantext, plus a number of dictionaries, lexis and other databases. These web available resources are operated and run through the potentialities and powerful capacities of a software called Stella, a search engine specially dedicated to textual databases and relying on a new theory of textual objects.
Maximal exercise, limb ischemia, and endothelial progenitor cells

Objectives: The concept of neovascularization in response to tissue ischemia was recently extended by the finding of postnatal vasculogenesis through circulating endothelial progenitor cells (EPCs). The aim of this study was to assess the role of acute ischemia for EPC mobilization in patients with peripheral arterial occlusive disease (PAOD) and in healthy volunteers. Methods: The number of circulating EPCs was analyzed by flow cytometry in PAOD patients (n = 23) with exercise-induced limb ischemia for up to 72 h after a maximal treadmill test and in healthy volunteers (n = 17) who underwent a 15-min suprasystolic occlusion of one lower extremity to induce limb ischemia. Plasma concentrations of vascular endothelial growth factor, basic fibroblast growth factor, tumor necrosis factor-α, and granulocyte macrophage-colony stimulating factor were determined by ELISA. Results: EPCs (CD 34 pos/KDRpos) increased significantly in both PAOD patients from 82 ± 20 to 256 ± 52 (P < 0.05) and healthy volunteers from 144 ± 39 to 590 ± 61 cells per 1 million events (P < 0.05) in response to induced ischemia, with a maximum after 24 h and returned to baseline within 72 h. The relative increase in EPC numbers was significantly lower in patients with PAOD as compared with healthy volunteers (P < 0.05). Plasma levels of vascular endothelial growth factor increased from 27.4 ± 3.1 to 126.4 ± 12 pg/ml in patients with PAOD (P < 0.05) and from 30.7 ± 6.1 to 134.1 ± 12.4 pg/ml in healthy volunteers (P < 0.05). Conclusion: Both patients with symptomatic PAOD and healthy volunteers respond to a single episode of limb ischemia with a time-dependent increase in circulating EPCs. The increase of EPC numbers in response to ischemia is reduced when vascular disease is present, underlining the reduced vasculogenic potential of patients with PAOD.
Is religiousness a correlate of absorption? Implications for psychophysiology, coping, and morbidity.

CONTEXT Evidence synthesized from social epidemiology, psychophysiology, and behavioral medicine suggests that religiousness may represent a significant correlate of absorption, a construct for which few if any psychosocial determinants have been identified. OBJECTIVE To examine the association between absorption and intrinsic and extrinsic religiousness. PARTICIPANTS 83 respondents of a self-administered survey of adult survivors of cancer or other life-threatening diseases, recruited from participants in a pilot study of psychosocial factors related to recovery from illness. MAIN MEASURES Tellegen Absorption Scale and Religious Orientation Scale. RESULTS Absorption, as assessed by the Tellegen Absorption Scale, was positively and significantly associated with intrinsic religiousness, as measured by the Religious Orientation Scale. Predominantly intrinsic subjects had absorption scores at least 20% higher than did predominantly extrinsic, proreligious, or nonreligious subjects. DISCUSSION Prior research has found that absorption and hypnotizability have psychophysiological correlates, and that religiousness shows protective effects against morbidity and mortality. In light of this work, the present findings suggest that certain religious cognitions, emotions, or experiences may generate an internally focused state that enhances health and attenuates disease through self-soothing psychophysiological mechanisms.
CT Angiography in the Rapid Triage of Patients with Hyperacute Stroke to Intraarterial Thrombolysis: Accuracy in the Detection of Large Vessel Thrombus

Purpose The purpose of this work was to evaluate the accuracy of CT angiography (CTA) for the detection of large vessel intracranial thrombus in clinically suspected hyperacute (<6 h) stroke patients. Method Forty-four consecutive intraarterial thrombolysis candidates underwent noncontrast CT followed immediately by CTA. Axial source and two-dimensional collapsed maximum intensity projection reformatted CTA images were rated for the presence or absence of large vessel occlusion. Five hundred seventy-two circle-of-Willis vessels were reviewed; arteriographic correlation was available for 224 of these. Results Sensitivity and specificity for the detection of large vessel occlusion were 98.4 and 98.1%; accuracy, calculated using receiver operating characteristic analysis, was 99%. Mean time for acquisition, reconstruction, and analysis of CTA images was approximately 15 min. Conclusion CTA is highly accurate for the detection and exclusion of large vessel intracranial occlusion and may therefore be valuable in the rapid triage of hyperacute stroke patients to intraarterial thrombolytic treatment.
Slalom: Coasting Through Raw Data via Adaptive Partitioning and Indexing

The constant flux of data and queries alike has been pushing the boundaries of data analysis systems. The increasing size of raw data files has made data loading an expensive operation that delays the data-to-insight time. Hence, recent in-situ query processing systems operate directly over raw data, alleviating the loading cost. At the same time, analytical workloads have increasing number of queries. Typically, each query focuses on a constantly shifting -- yet small -- range. Minimizing the workload latency, now, requires the benefits of indexing in in-situ query processing. In this paper, we present Slalom, an in-situ query engine that accommodates workload shifts by monitoring user access patterns. Slalom makes on-the-fly partitioning and indexing decisions, based on information collected by lightweight monitoring. Slalom has two key components: (i) an online partitioning and indexing scheme, and (ii) a partitioning and indexing tuner tailored for in-situ query engines. When compared to the state of the art, Slalom offers performance benefits by taking into account user query patterns to (a) logically partition raw data files and (b) build for each partition lightweight partition-specific indexes. Due to its lightweight and adaptive nature, Slalom achieves efficient accesses to raw data with minimal memory consumption. Our experimentation with both micro-benchmarks and real-life workloads shows that Slalom outperforms state-of-the-art in-situ engines (3 -- 10×), and achieves comparable query response times with fully indexed DBMS, offering much lower (∼ 3×) cumulative query execution times for query workloads with increasing size and unpredictable access patterns.
Development of an Ontology to Recommend Exercises from Conceptual Maps

The recommendation of exercise plans requires several variables to be considered (e.g., patient's conditions and preferences) and are normally complex to analyze. To facilitate this analysis we proposed the creation of an ontology to assist professionals to recommend exercises. We interviewed 2 experts and this resulted in IDEF diagram and conceptual map. The conceptual map proved to be the preferred way that experts gained more understanding compared with the IDEF diagram. In addition, we also used the conceptual map to validate the formal structure of experts' ideas. From the conceptual map we created an ontology that is being reviewed. After this, we plan to incorporate the ontology into a decision support system that will assist professionals to recommend exercises for their patients.