Miracle-dz/UniFAR-FTUs huggingface dataset:
Kinetic analysis by in vivo 31P nuclear magnetic resonance of internal Pi during the uptake of sn-glycerol-3-phosphate by the pho regulon-dependent Ugp system and the glp regulon-dependent GlpT system

When sn-glycerol-3-phosphate (G3P) is taken up exclusively by the pho regulon-dependent Ugp transport system, it can be used as the sole source of Pi but not as the sole source of carbon. We had previously suggested that the inability of G3P to be used as a carbon source under these conditions is due to trans inhibition of G3P uptake by internal Pi derived from the degradation of G3P (P. Brzoska, M. Rimmele, K. Brzostek, and W. Boos, J. Bacteriol. 176:15-20, 1994). Here we report 31P nuclear magnetic resonance measurements of intact cells after exposure to G3P as well as to Pi, using different mutants defective in pst (high-affinity Pi transport), ugp (pho-dependent G3P transport), glpT (glp-dependent G3P transport), and glpD (aerobic G3P dehydrogenase). When G3P was transported by the Ugp system and when metabolism of G3P was allowed (glpD+), Pi accumulated to about 13 to 19 mM. When G3P was taken up by the GlpT system, the preexisting internal Pi pool (whether low or high) did not change. Both systems were inversely controlled by internal Pi. Whereas the Ugp system was inhibited, the GlpT system was stimulated by elevated internal Pi.
Multidimensional Poverty Analysis at the Local Level in Northwest Yunnan Province, China: Some Insights and Implications

ABSTRACT This study is conducted in ethnic plateau of Deqin County of Yunnan Province, China, to understand the multidimensional poverty status using the Alkire–Foster Method with contextualized indicators linked to the dimensions of Education, Health, Living Conditions, Assets, and Social Relations. Using data gathered from 647 households in eight townships, the multidimensional poverty index (MPI) is calculated. Generally, Assets and Living Conditions are the two dimensions lacking in most households. This research takes the perception of the household into account, and compares the MPI results with the household self-assessment results.
Extracting HPSG Lexicon from Arabic VerbNet

This paper presents the construction of a HPSG lexicon of Arabic verbal entities, automatically inferred from the Arabic VerbNet, a large coverage verb lexicon where verbs are classiﬁed using syntactic alternations. We discuss the main verb speciﬁcation along with the relation of the syntactic and semantic levels of representation within the HPSG framework. Extensive analysis of the Arabic VerbNet classes has led to the adoption of a ﬁnite set of mapping rules between AVN classes and HPSG subcategorization and semantics descriptions covering the majority of the verbal tokens. We employed the adopted mapping rules to extract the syntactic and semantic data from AVN and ﬁnally, we describe the resulting TDL descriptions in which the lexicon has been encoded.
Exogenous glutathione enhances cadmium accumulation and alleviates its toxicity in Populus × canescens

Glutathione (GSH) plays an important role in cadmium (Cd) tolerance in woody plants, but the underlying mechanisms remain largely unknown. To elucidate the physiological and transcriptional regulation mechanisms of GSH-mediated Cd tolerance in woody plants, we exposed Populus × canescens (Ait.) Smith saplings to either 0 or 75 μM Cd together with one of three external GSH levels. Glutathione treatments include buthionine sulfoximine (BSO, an inhibitor of GSH biosynthesis), no external GSH and exogenous GSH. External GSH resulted in higher Cd2+ uptake rate in the roots, greater Cd amount in poplars, lower Cd-induced H2O2 levels in the roots, and higher contents of endogenous GSH in Cd-treated roots and leaves. Furthermore, external GSH led to upregulated transcript levels of several genes including zinc/iron regulated transporter related protein 6.2 (ZIP6.2) and natural resistance-associated macrophage protein 1.3 (NRAMP1.3), which probably take part in Cd uptake, glutathione synthetase 2 (GS2) implicated in Cd detoxification, metal tolerance protein 1 (MTP1) and ATP-binding cassette transporter C3 (ABCC3) involved in Cd vacuolar accumulation in the roots, γ-glutamylcysteine synthetase (ECS) and phytochelatin synthetase family protein 1 (PCS1) involved in Cd detoxification, and oligopeptide transporter 7 (OPT7) probably implicated in Cd detoxification in the leaves of Cd-exposed P. × canescens. In contrast, BSO often displayed the opposite effects on Cd-triggered physiological and transcriptional regulation responses in poplars. These results suggest that exogenous GSH can enhance Cd accumulation and alleviate its toxicity in poplars. This is probably attributed to external-GSH-induced higher net Cd2+ influx in the roots, greater Cd accumulation in aerial parts, stronger scavenging of reactive oxygen species, and transcriptional overexpression of several genes involved in Cd uptake, detoxification and accumulation.
The separation of harvested cells from microcarriers: a comparison of methods.

Two techniques are described which have been designed to separate harvested cells from microcarriers. The requirements of efficient recovery and high viability of the cells are met by both procedures. Differences both in size and density between cells and microcarriers allow separations which are based on differential centrifugation or filtration. After trypsinizing the cells from the microcarriers, separation was performed by either ; a) low-speed centrifugation on Ficoll-Paque or, b) filtration through nylon meshes. The methodologies for both techniques are presented. Up to 78% of the total cells were recovered by discontinuous gradient centrifugation using Ficoll-Paque. similar results were obtained when separating Vero, BS-C-1 and MRC-5 cells from Cytodex 1, Cytodex 2 and Cytodex 3 microcarriers. Equally high recoveries were obtained by filtration through an 88 micron pore size nylon mesh. Data are presented for the separation of both Vero and HeLa cells from all 3 types of microcarriers after filtration through 53 and/or 88 micron meshes. Greater than 92% viability of the recovered cells was consistently obtained and their growth properties upon subsequent reculturing were unaffected by either separation procedure. Differential sedimentation by unit gravity provides adequate cell recovery for many applications, but yields are significantly increased by using one or other of the methods described here. Both techniques are rapid and efficient. In addition, differential centrifugation provides a concentrated suspension of the recovered cells, while the nylon meshes for filtration are reusable and can be autoclaved at least 5 times. (ABSTRACT TRUNCATED AT 250 WORDS)
Typology and Success Factors of Collaboration for Sustainable Growth in the IT Service Industry

Recently, innovative changes in information technology (IT) trends, such as cloud computing and deep learning, have led IT companies to focus on collaboration for sustainable growth. This paper investigates collaboration strategies and success factors for IT service companies via a survey-based empirical study of Korean leading IT firms. Four types of collaboration were identified by considering the types of customer relationship and the target market: offshore, joint venture, collaboration with small and medium-sized enterprises (SMEs), and partnership with major local firms. Then, based on a Plan-Do-See management activity process, this paper considers success factors in the planning process and collaboration process, and analyzes an impact of these factors on collaboration performance such as financial performance, process innovation, improving competitiveness, and technology acquisition. As a result, the success factors differ according to the types of performance measures as well as the collaboration types. In particular, the characteristics of partners positively influence competitiveness in captive and global markets, while they improve process innovation in open and domestic markets. This study attempts to provide insight for companies in the IT service industry about how collaboration activities could enhance performance, depending on the alliance types.
Methodology for the Design and Evaluation of Self-Healing LTE Networks

Self-healing networks aim to detect cells with service degradation, identify the fault cause of their problem, and execute compensation and repair actions. The development of this type of automatic system presents several challenges to be confronted. The first challenge is the scarce number of historically reported faults, which greatly complicates the evaluation of novel self-healing techniques. For this reason, in this paper, a system model to simulate faults in Long-Term Evolution (LTE) networks, along with their most significant key performance indicators, is proposed. Second, the expert knowledge required to build a self-healing system is usually not documented. Therefore, in this paper, a methodology to extract this information from a collection of reported cases is proposed. Finally, following the proposed methodology, an automatic fuzzy-logic-based system for fault identification in LTE networks is designed. Evaluation results show that the fuzzy system provides fault identification with a high success rate.
Who Am I Here? Disrupted Identities and Gentle Shifts When Teaching in Cyberspace.

Teacher identity has most often been studied in reference to teacher candidates (Beauchamp & Thomas, 2009; Franzak, 2002; Freese, 2006; Hoban, 2007; Murrell, Diez, Feiman-Nemser, & Schussler, 2010; Sachs, 2005), in the area of literacy learning and its connection to teacher identity (Moje, 2008; Spitler, 2009, 2011), and in regards to teachers’ professional development (Day, Stobart, Sammons, & Kingston, 2006; Trevitt & Perera, 2009; Watson, 2006). The research on teacher candidate and in-service teacher identity exists in contrast to the dearth of research on teacher identity in higher education, which has created a gap in establishing teacher identity issues across all forms of educational experiences. With the move to more online learning in higher education (Daughtery & Funke, 1998; Kazar & Eckel, 2002), specifically in the area of teacher education, studying the identity of teacher educators as both instructors and as literacy learners is an area of research that requires attention. Addressing identities in an online environment allows for a greater understanding of teachers’ relationships to the modality. In essence, do the tools teacher educators use impact their identities as teachers within a classroom or course? By exploring the use of technology and its impact on our relationships with students, the authors of this paper embarked upon a yearlong self-study as a reflective community of practice (Lave & Wenger, 1991; Wenger, 1998). In this article, we share our self-study process and current understandings about what it has meant to move from our physical, on campus identities toward a more virtual presence with students. We begin with an exploration of what it means to teach, what the concept of “identity” might mean, and what the world of online teaching has become in the realm of higher education. We end this article with a discussion of the impact our disrupted identities have had on our program and the reflections we continue to ponder.
Lessons on Running a Laboratory Experiment (in Graduate School)

ABSTRACT Experimentation has taken on a new life in political science. As the use of experimental methods proliferates, it is important for researchers to share their experiences and best practices, particularly with early-career researchers. This article provides reflections from practical experience in the laboratory, particularly geared toward graduate students and early-career researchers who are conducting their first laboratory experiment. These lessons do not apply only to first-time experimenters. Experiences are presented regarding time management, using confederates and deception, incentivizing participation, and keeping a laboratory notebook. Finally, early-career researchers are encouraged to “go for it” if the methods are appropriate to their research question.
Inducible CD40 expression mediates NFκB activation and cytokine secretion in human colonic fibroblasts

Background: CD40 has been shown to be a functional activation antigen on a variety of cell types involved in immune responses. As intestinal fibroblasts and myofibroblasts may play a role during mucosal inflammation, we investigated the functional consequences of CD40 induction in primary cultures of human colonic fibroblasts. Methods: Primary colonic lamina propria fibroblasts (PCLF) were isolated from endoscopic biopsies and surgical specimens. Cultures were used between passages 3 and 9. CD40 surface display was determined by FACS analysis and mRNA expression by reverse transcription-polymerase chain reaction. Secretion of cytokines was determined by ELISA. Nuclear factor κB (NFκB) activation was shown by electrophoretic mobility shift assay (EMSA). Results: After priming with interferon γ (IFN-γ) (200 U/ml) for 72 hours, five of eight tested PCLF cultures showed induction of CD40 surface display (up to 10-fold). Induction of CD40 mRNA expression was demonstrated by semiquantitative polymerase chain reaction. In the responder-PCLF cultures, IFN-γ alone caused a 1.5–5-fold increase in interleukin (IL)-8 secretion. Addition of 1 ng/ml CD40L was sufficient to achieve a further increase in IL-8, IL-6, or monocyte chemotactic protein 1 (MCP-1) secretion (2.5–18-fold of controls). Incubation with CD40L alone without priming with IFN-γ had no effect. The proteasome inhibitor N-acetyl-leucinyl-leucinyl-norleucinal (ALLN 100 µM) reduced IFN-γ/CD40L mediated cytokine induction, suggesting participation of NFκB, which was directly demonstrated by EMSA. CD4+ T cells induced MCP-1 secretion by PCLF, which was prevented by addition of an excess of CD40-IgG fusion protein. CD40 expression on PCLF could also be demonstrated in vivo by immunohistochemistry. Conclusion: The CD40-CD40L pathway augments mucosal inflammatory responses via mucosal PCLF. CD40-CD40L mediated T cell/PCLF interactions could play an important role during intestinal mucosal inflammation.
Near-Field Intensity Enhancement of a nano-antenna above an inverted Bragg Reflector

The Near-Field Intensity Enhancement (NFIE) of a Distributed Bragg Reflector has been calculated at the presence of a constructive interference at the surface of a Bragg reflector. The Bragg reflector has been constructed using SiO2/GaAs with respective refractive indices of n1=1.5 and n2=3.6. A reflectivity of 0.99986 has been simulated with an electric field enhancement approaching 2. The Bragg reflector is coupled with a Gold dipole nanoantenna to obtain a significant NFIE that is 14 times stronger than that of a Gold dipole nanoantenna above a pure silica (SiO2) surface. This significant increase is shown to be the result of the coupling effect between the Gold dipole and its mirror image.
Domino-like propagation of collective U-turns in fish schools

Moving animal groups such as schools of fish or flocks of birds often undergo sudden collective changes of their travelling direction as a consequence of stochastic fluctuations in heading of the individuals. However, the mechanisms by which these behavioural fluctuations arise at the individual level and propagate within a group are still unclear. In the present study, we combine an experimental and theoretical approach to investigate spontaneous collective U-turns in groups of rummy-nose tetra (Hemigrammus rhodostomus) swimming in a ring-shaped tank. U-turns imply that fish switch their heading between the clockwise and anticlockwise direction. We reconstruct trajectories of individuals moving alone and in groups of different sizes. We show that the group decreases its swimming speed before a collective U-turn. This is in agreement with previous theoretical predictions showing that speed decrease facilitates an amplification of fluctuations in heading in the group, which can trigger U-turns. These collective U-turns are mostly initiated by individuals at the front of the group. Once an individual has initiated a U-turn, the new direction propagates through the group from front to back without amplification or dampening, resembling the dynamics of falling dominoes. The mean time between collective U-turns sharply increases as the size of the group increases. We develop an Ising spin model integrating anisotropic and asymmetrical interactions between fish and their tendency to follow the majority of their neighbours nonlinearly (social conformity). The model quantitatively reproduces key features of the dynamics and the frequency of collective U-turns observed in experiments.
Going out of the Great Recession? Contrast between the United States and Europe: Proposed work from economic history, 1960–2014

ABSTRACT In recent work, the authors have proposed to the United States a model that explains the trend behavior of the rate of profit from share surplus, capital productivity, and the coefficient of financialization. The main results of the explanatory model allow the authors to affirm that with the change of control of Keynesianism to neoliberalism since 1980, there has been a substantial fall in the profit rate to half the values achieved in the years of Keynesian regulation (1945–1973). This significant fall in the level of benefits is due to a substantial fall in capital productivity. The authors are currently working on adapting the explanatory model for the U.S. economy to the main countries of the European Union (Germany, France, Italy, United Kingdom, and Spain). The results show that the pattern of behavior of the variables described in the reference country—the world capitalist economic system, the United States—is repeated more or less precisely in the main countries of the European Union; Germany, France, Italy, United Kingdom, and Spain.
PERSUASIVE TECHNOLOGY AND GAMIFICATION AT THE WORKPLACE: ENGAGING EMPLOYEES IN EFFECTIVE DOCUMENTATION OF ANALYSIS AND EVALUATIONS

II ACKNOWLEDGEMENT . III CHAPTER 1: INTRODUCTION . 1 PROBLEM STATEMENT AND RELEVANCE . 3 THESIS OVERVIEW . 6 CHAPTER 2: BACKGROUND AND RELATED WORK . 7 2.1 MOTIVATION AND BEHAVIOR CHANGE (INTRINSIC AND EXTRINSIC MOTIVATION). 7 2.2 WORKPLACE MOTIVATION AND ENGAGEMENT . 9 2.3 PERSUASIVE TECHNOLOGIES . 11 2.4 GAMIFICATION AS A PERSUASIVE TECHNOLOGY . 16 2.5 PERSUASIVE TECHNOLOGY AND GAMIFICATION AT THE WORKPLACE . 18 2.5.1 Workplace Persuasive Technology and Gamification Frameworks . 19 2.5.2 Examples of Persuasive Technology and Gamification at the Workplace . 21 2.5. 3 Ethical Considerations of Persuasive Technology and Gamification at Workplaces . 22 2.5. 3 Gaps . 24 CHAPTER 3: RESEARCH METHOD . 27 3.1 USER STUDIES . 28 3.1. 1 Data Collection . 29 3.1. 2 Participants . 33 3.1. 3 Data Analysis . 34 CHAPTER 4: ABA FRONT-LINE STAFF STUDY . 36 4.1 PARTICIPANTS . 36 4.2 INTERNAL CONSISTENCY AND CONSTRUCT PERFORMANCE . 37 4.3 EMPLOYEE SUSCEPTIBILITY TO CIALDINI’S PERSUASIVE PRINCIPLES . 37 4.4 EMPLOYEE SUSCEPTIBILITY TO KUKKONEN’S PERSUASIVE STRATEGIES . 39 4.5 DISCUSSION. 41 4.5.1 Susceptibility to Cialdini’s Persuasive Principles. 41 4.5.2 Susceptibility to Social Influence Persuasive Strategies. 41 4.6 DESIGN REQUIREMENTS. 42 4.7 LIMITATIONS AND FUTURE WORK . 43 CHAPTER 5: GRADUATE STUDENT ASSISTANTS STUDY . 44 5.1 METHODS . 44 5.1. 1 Tools . 44 5.1. 2 Procedure . 45 5.1. 3 Participants . 45 5.2 RESULTS . 46 5.2.1 Internal Consistency and Instruments/Construct Performance . 46 5.2.2 Workplace Basic Need Satisfaction (WBNS) . 48 5.2.3 Work Tasks Motivation Scale for Teachers (WTMST) . 49 5.2.4 Workplace Motivating Factors . 52
Serum gamma-glutamyltransferase predicts non-fatal myocardial infarction and fatal coronary heart disease among 28,838 middle-aged men and women.

AIMS Serum gamma-glutamyltransferase (GGT) concentration may be involved in atherosclerosis. This study examined if serum GGT predicted coronary heart disease (CHD), especially differentiating non-fatal myocardial infarction (MI) and fatal CHD event, among the general population or participants with type-2 diabetes. METHODS AND RESULTS A prospective study of 28,838 Finnish men and women aged 25-74 years was performed (1467 incident CHD cases; a median follow-up time of 11.9 years). Serum GGT cutpoints were the 25th, 50th, 75th, and 90th sex-specific percentiles. After adjustment for known cardiovascular risk factors, compared with the lowest GGT category, hazard ratios (HR) were 1.15, 1.25, 1.27, and 1.57 among men and 1.03, 1.22, 1.32, and 1.44 among women in other four GGT categories (P for trend <0.01, respectively). However, stronger associations were observed among subjects aged <60 and among alcohol drinkers. The strength of association was similar for non-fatal MI and for fatal CHD. Among subjects with type-2 diabetes, the corresponding adjusted HRs were 1.29, 1.57, 1.88, and 1.78 (P trend=0.03, men and women combined). CONCLUSION This study suggests an independent mechanism linking serum GGT to CHD among general population. Even though the strength of association appeared to be modest among all subjects, stronger associations were observed among subjects aged <60 and among alcohol drinkers. Especially, measurement of serum GGT among type-2 diabetics may be helpful to predict the future risk of CHD.
Detection and recognition of traffic signs based on RGB to red conversion

This paper presents a different approach for detecting and recognizing traffic signs. Traffic signs are designed such that they can be easily observed by humans because of their shape and shading which are not precisely the same as surrounding environment. Traffic sign recognition is a fundamental component in autonomous cars and it can similarly be utilized as an integral part of driver assistance frameworks. The system is designed to perform two tasks, traffic sign detection and recognition. The algorithms demonstrated include RGB to Red conversion for getting the red component in the image, filters which perform the task of noise reduction as well as edge detection, thresholding and segmentation to get the desired traffic sign from image and then an artificial neural network for recognition. The neural network used here is a Single Layer Perceptron neural network. Implementation of the system has been successfully done in Matlab and it works quite productively and efficiently with the database of Indian traffic signs created as a part of the project.
An assessment of Manasika Bhavas in menopausal syndrome and its management

The present clinical trial was conducted to evaluate the efficacy of Shirodhara and Saraswatarishta as compared to hormone replacement therapy (HRT) in the management of menopausal syndrome, along with the assessment of Manasika Bhavas. The subjects were randomly divided into three groups. A total 48 patients were selected for the study, out of which 43 patients completed the study. Specialized Ayurvedic rating scales like Manasa Pariksha Bhavas as well as the Hamilton Anxiety Rating Scale, Hamilton Depression Rating scale, and Menopause Rating Scale were adopted to assess the effect of therapy. The effects were examined on the chief complaints as well as the associated complaints. Patients undergoing Shirodhara had better relief of the disturbances of Manasa Bhavas and psychic symptoms of menopause as compared to the other two groups. Saraswatarishta also showed encouraging results in managing the associated somatic symptoms and the psychic symptoms. Hence, we conclude that it can be used as an alternative therapy to HRT.