Miracle-dz/UniFAR-FTUs huggingface dataset:
Group Dancing Mobile Flower Robots with Moving Mechanism, Mobility and Localization Functions

Recently, the diverse robots with flower shape have been developed for use as interior, HRI, performance, and home appliance. In this research, we proposed new type mobile flower robots which have flower-type design, mobility, and localization. Especially, the flower shape robot was designed to mimic the swaying of the branches and the blooming of the flowers. The branches and the flowers were actuated by tendon mechanism. And, the flower robots have the mobile platform which consists of three-wheels with the steering ability, respectively. The mobile platforms of the flower robot can locomote to the desired direction and track to the desired path. The localization of multi mobile flower robot was based on the smart floor using passive RFID tags and the extended Kalman filter to estimate of location(x, y) under nondetection of RFID tags. RFID tags which are equilateral triangulation grid pattern are distributed under the surface of the smart floor and each of RFID tags is able to present the unique information of location(x, y). In the experiment system RFID tags are mainly used for identifying location of the mobile flower robot. The extended Kalman filter is used to estimate the location(x, y) of the mobile flower robots when the robots are middle of tags or out of the range of RFID tags. In addition, the heading (theta) of mobile flower robot is estimated by the angle sensor. Finally, by using the motion of the branches and flowers and the mobility of the flower robots, the group dancing of the mobile flower robots was demonstrated. These group dancing mobile flower robots with the moving mechanism, the mobility, and the localization functions can be used for a public performance and an exhibition.
Draft Genome Sequence of Dihydroxyacetone-Producing Gluconobacter thailandicus Strain NBRC 3255

ABSTRACT Here, we report the draft genome sequence of the acetic acid bacterium Glucnobacter thailandicus strain NBRC 3255. The draft genome sequence is composed of 109 contigs in 3,305,227 bp and contains 3,225 protein-coding genes. Two paralogous sets of sldAB operons, which are responsible for dihydroxyacetone production from glycerol, were identified.
Extracellular 8-oxo-dG as a sensitive parameter for oxidative stress in vivo and in vitro

8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) is one of the mutagenic base modifications produced in DNA by the reaction of reactive oxygen species. The biological significance of 8-oxo-dG is shown by the existence of repair pathways that are able to recognize and remove this lesion from both DNA and the nucleotide pool. The final outcome of these evolutionarily conserved repair mechanisms in man is excretion of 8-oxo-dG/8-oxo-Gua from the intracellular to extracellular milieu including the blood plasma and urine. The aim of this investigation was to establish dose response relations for radiation-induced appearance of extracellular 8-oxo-dG in cellular model systems. Here we report on excretion of 8-oxo-dG after in vitro irradiation of whole blood and isolated lymphocytes with clinically relevant doses. We find that this excretion is dependent on dose and individual repair capacity, and that it saturates above doses of 0.5–1 Gy of gamma radiation. Our data also suggest that the nucleotide pool is a significant target that contributes to the levels of extracellular 8-oxo-dG; hence the mutagenic target for oxidative stress is not limited to the DNA molecule only. We conclude that extracellular 8-oxo-dG levels after in vitro irradiation have a potential to be used as a sensitive marker for oxidative stress.
Clinical significance of interleukin-6 and inducible nitric oxide synthase in ketamine-induced cystitis

Ketamine is an ionotropic glutamatergic N-methyl-D-aspartate receptor antagonist, which is widely used among recreational drug abusers. Ketamine abusers exhibit substantially reduced bladder capacity, which can lead to urinary frequency. The molecular pathogenesis of ketamine-induced cystitis has been scarcely reported. Given previous clinical findings, it may be hypothesized that pathological alterations in smooth muscle cells (SMCs) of the urinary bladder serve a crucial role in the mechanism underlying cystitis. In the present study, two lineages of SMCs, one from differentiated foreskin-derived fibroblast-like stromal cells and the other from cultured normal aortic SMCs, were used to study ketamine-induced molecular alterations. Polymerase chain reaction was used to study the effects of ketamine on oxidative stress. The effects of adjuvant chemo-therapy with cyclophosphamide (CTX) were also investigated. The results indicated that the expression levels of interleukin-6 and inducible nitric oxide synthase (iNOS) were decreased, whereas collagen expression and deposition were increased in ketamine-treated SMCs. Conversely, treatment with CTX restored the expression of iNOS, which may prevent or limit oxidative damage. In conclusion, the present study demonstrated that ketamine may induce several molecular alterations in SMCs and these changes may be associated with the clinical symptoms observed in ketamine abusers. In addition, the specific chemotherapeutic agent CTX may reverse these ketamine-induced aberrations.
Clustered PV inverters in LV networks: An overview of impacts and comparison of voltage control strategies

High penetration of photovoltaic (PV) inverters in low voltage (LV) distribution network challenges the voltage stability due to interaction between multiple inverters and grid. As the main objective is to provide more power injection from VSC-based PV inverters, grid stability, reliability and power quality must be maintained or improved by adding cooperative control features to the grid-connected inverters. This paper first gives an overview of bilateral impacts between multiple distributed generations (DG) and grid. Regarding of these impacts, recent advances in static grid voltage support functionalities to increase penetration level are compared considering voltage rise limitation. Steady-state simulation study is realized in PSCAD/EMTDC and the results are discussed in terms of total generation efficiency.
Photoluminescence of semiconductor nanocrystal quantum dots at 1550 nm wavelength in the core of photonic bandgap fiber

Photoluminescence is observed from PbSe nano-crystal quantum dots at 1550 nm wavelength filling in the core of a 1 m long photonic bandgap fiber. Photoluminescence at the peak wavelength of 1554 nm is observed with 1535 nm, 10 mW pumping.
Geographical Differentials in the Socioeconomic Status of Puerto Ricans: Human Capital Variations and Labor Market Characteristics 1

This article examines the role of human capital and labor market characteristics in explaining geographical and individual differentials in socioeconomic outcomes of Puerto Rican women. The better socioeconomic performance of Puerto Ricans outside the Northeast can be in part related to their larger amount of human capital. Labor market characteristics also play a role, but their effects are generally small. Net of other characteristics, Northeast residence reduces labor force participation, increases female headship, but reduces welfare use. Of all groups examined, recent migrants from Puerto Rico located in the Northeast show the poorest socioeconomic outcomes.
Strategies to Prevent Central Line–Associated Bloodstream Infections in Acute Care Hospitals

Previously published guidelines are available that provide comprehensive recommendations for detecting and preventing healthcare-associated infections. The intent of this document is to highlight practical recommendations in a concise format designed to assist acute care hospitals in implementing and prioritizing their central line–associated bloodstream infection (CLABSI) prevention efforts. Refer to the Society for Healthcare Epidemiology of America/Infectious Diseases Society of America “Compendium of Strategies to Prevent Healthcare-Associated Infections” Executive Summary and Introduction and accompanying editorial for additional discussion. 1. Patients at risk for CLABSIs in acute care facilities a. Intensive care unit (ICU) population: The risk of CLABSI in ICU patients is high. Reasons for this include the frequent insertion of multiple catheters, the use of specific types of catheters that are almost exclusively inserted in ICU patients and associated with substantial risk (eg, arterial catheters), and the fact that catheters are frequently placed in emergency circumstances, repeatedly accessed each day, and often needed for extended periods. b. Non-ICU population: Although the primary focus of attention over the past 2 decades has been the ICU setting, recent data suggest that the greatest numbers of patients with central lines are in hospital units outside the ICU, where there is a substantial risk of CLABSI. 2. Outcomes associated with hospital-acquired CLABSI a. Increased length of hospital stay b. Increased cost; the non-inflation-adjusted attributable cost of CLABSIs has been found to vary from $3,700 to $29,000 per episode
An improved screening technique for isolation ofNocardia species from sputum specimens

&NA; Three selective isolation media and the paraffin baiting technique were compared with conventional culture (Sabouraud dextrose agar without antibiotic supplement) for the ability to grow and detect nocardiae from simulated sputum specimens. Modified Thayer‐Martin medium, incorporating vancomycin, colistin, nystatin and trimethoprim as selective agents, produced the highest recovery rate and with the greatest suppression of normal respiratory tract flora. A clinical evaluation using a screening programme devised for a busy diagnostic microbiology laboratory was performed on 1600 sputum specimens. Inoculating sputum on modified Thayer‐Martin medium and extending the initial incubation period of 3 days at 35°C under 10% carbon dioxide to a further 3 weeks at room temperature in a candle jar, led to the diagnoses, which otherwise would have been missed, of pulmonary nocardiosis in 3 patients and pulmonary infections due to Neisseria meningitidis, Pseudomonas cepacia, and Serratia marcescens in a further 22 patients.
BCL-2 family members and the mitochondria in apoptosis.

A variety of physiological death signals, as well as pathological cellular insults, trigger the genetically programmed pathway of apoptosis (Vaux and Korsmeyer 1999). Apoptosis manifests in two major execution programs downstream of the death signal: the caspase pathway and organelle dysfunction, of which mitochondrial dysfunction is the best characterized (for reviews, see Green and Reed 1998; Thornberry and Lazebnik 1998). As the BCL-2 family members reside upstream of irreversible cellular damage and focus much of their efforts at the level of mitochondria, they play a pivotal role in deciding whether a cell will live or die (Fig. 1). The founder of this family, the BCL-2 proto-oncogene, was discovered at the chromosomal breakpoint of t(14;18) bearing human B-cell lymphomas. The BCL-2 family of proteins has expanded significantly and includes both proas well as anti-apoptotic molecules. Indeed, the ratio between these two subsets helps determine, in part, the susceptibility of cells to a death signal (Oltvai et al. 1993) (Fig. 1). An additional characteristic of the members of this family is their frequent ability to form homoas well as heterodimers, suggesting neutralizing competition between these proteins. A further characteristic of probable functional significance is their ability to become integral membrane proteins. BCL-2 family members possess up to four conserved BCL-2 homology (BH) domains designated BH1, BH2, BH3, and BH4, which correspond to a-helical segments (Adams and Cory 1998; Kelekar and Thompson 1998; Reed 1998) (Fig. 2). Many of the anti-apoptotic members display sequence conservation in all four domains. The pro-apoptotic molecules frequently display less sequence conservation of the first a-helical segment, BH4. Deletion and mutagenesis studies argue that the amphipathic a-helical BH3 domain serves as a critical death domain in the pro-apoptotic members. This concept is supported by an emerging subset of “BH3-domain-only” members who display sequence homology only within the BH3 domain and to date are all pro-apoptotic. However, the three-dimensional structure of at least one BH3-domainonly molecule, BID, demonstrates a very similar overall a-helical content to the anti-apoptotic molecule BCL-XL (Chou et al. 1999; McDonnell et al. 1999). Many BCL-2 family members also contain a carboxy-terminal hydrophobic domain, which in the case of BCL-2 is essential for its targeting to membranes such as the mitochondrial outer membrane (Nguyen et al. 1993).
Natural designs for manipulating the appearance of surfaces

The surfaces of animals and plants are frequently adorned with a wealth of pattern, colour, and texture. Some insects for example, exhibit uniformly‐coloured unpatterned white surfaces; some display bright iridescent blue or green hues; others may present varying shades of black, brown or selected combinations of spectral colours. Additionally, unseen by human observers, certain species’ wing or body surfaces may produce strong ultra‐violet or polarisation signatures. This review will introduce the characteristics of structural colour effects and describe examples of them in the natural world. It will summarise some of the recent work on a range of insect species, focussing predominantly on Lepidoptera as an exemplar order.
Comparing of ARIMA and RBFNN for short-term forecasting

Based on a combination of an autoregressive integrated moving average (ARIMA) and a radial basis function neural network (RBFNN), a time-series forecasting model is proposed. The proposed model has examined using simulated time series data of tourist arrival to Indonesia recently published by BPS Indonesia. The results demonstrate that the proposed RBFNN is more competent in modelling and forecasting time series than an ARIMA model which is indicated by mean square error (MSE) values. Based on the results obtained, RBFNN model is recommended as an alternative to existing method because it has a simple structure and can produce reasonable forecasts.
Large-scale Proteomics Combined with Transgenic Experiments Demonstrates An Important Role of Jasmonic Acid in Potassium Deficiency Response in Wheat and Rice

Potassium (K+) is the most abundant inorganic cation in plants, and molecular dissection of K+ deficiency has received considerable interest in order to minimize K+ fertilizer input and develop high quality K+-efficient crops. However, the molecular mechanism of plant responses to K+ deficiency is still poorly understood. In this study, 2-week-old bread wheat seedlings grown hydroponically in Hoagland solution were transferred to K+-free conditions for 8 d, and their root and leaf proteome profiles were assessed using the iTRAQ proteome method. Over 4000 unique proteins were identified, and 818 K+-responsive protein species showed significant differences in abundance. The differentially expressed protein species were associated with diverse functions and exhibited organ-specific differences. Most of the differentially expressed protein species related to hormone synthesis were involved in jasmonic acid (JA) synthesis and the upregulated abundance of JA synthesis-related enzymes could result in the increased JA concentrations. Abundance of allene oxide synthase (AOS), one key JA synthesis-related enzyme, was significantly increased in K+-deficient wheat seedlings, and its overexpression markedly increased concentrations of K+ and JA, altered the transcription levels of some genes encoding K+-responsive protein species, as well as enhanced the tolerance of rice plants to low K+ or K+ deficiency. Moreover, rice AOS mutant (osaos) exhibited more sensitivity to low K+ or K+ deficiency. Our findings could highlight the importance of JA in K+ deficiency, and imply a network of molecular processes underlying plant responses to K+ deficiency.
Isolation of an unusual mycobacterium from an AIDS patient

A mycobacterium isolated from a clinical sample of an AIDS patient was identified as Mycobacterium interjectum by direct 16S rRNA sequence determination. High-performance liquid chromatography, however, revealed a mycolic acid pattern which was different from the one shared by the previously analyzed strains of this species.
Comparison of four immunohistochemical tests and FISH for measuring Her2 expression in gastric carcinomas

Aim: Accurate testing for HER2 is essential to identify patients who are likely to benefit from trastuzumab therapy. Although comparative studies on the performance of commercially available immunohistochemistry (IHC) antibodies for HER2 have been performed in breast cancer, similar studies have not been done in gastric carcinoma (GC) thus far. Methods: Quadruplicate tissue microarrays (TMAs) of 289 advanced GCs were immunostained with HercepTest, A0485, 4B5 and CB11, and the results were compared to those of gene amplification by fluorescence in situ hybridisation (FISH). Results: HER2 was amplified by FISH in 38 (13.2%) GCs. HER2 overexpression (2+ or 3+) was observed in 40 (13.8%) cases by HercepTest, 46 (15.9%) by A0485, 40 (13.8%) by 4B5 and 27 (9.3%) by CB11. The sensitivity and specificity of IHC compared to FISH was 78.9%/96% for HercepTest, 86.5%/94.4% for A0485, 76.3%/95.6% for 4B5 and 60.5%/98.4% for CB11. Focal HER2 gene amplification was more common in GCs with no (0 and 1+) or low (2+) HER2 expression. Discordant IHC results were seen in 23 cases (8.0%) with the four antibodies. All HER2 3+ cases (n = 22) by HercepTest were positive with A0485 and 4B5, while one was negative with CB11. CB11 was found to be negative in 10 HercepTest 2+ and FISH+ cases. Conclusion: Focal HER2 gene amplification is more common in GCs with no or low expression of HER2. Although the specificity and positive predictive value of CB11 for FISH is highest, it lacks sufficient sensitivity to be used as a first line screening test to assess HER2 status in GCs.
Reduced Maximal Inhibition in Phenotypic Susceptibility Assays Indicates that Viral Strains Resistant to the CCR5 Antagonist Maraviroc Utilize Inhibitor-Bound Receptor for Entry

ABSTRACT Maraviroc is a CCR5 antagonist in clinical development as one of a new class of antiretrovirals targeting human immunodeficiency virus type 1 (HIV-1) coreceptor binding. We investigated the mechanism of HIV resistance to maraviroc by using in vitro sequential passage and site-directed mutagenesis. Serial passage through increasing maraviroc concentrations failed to select maraviroc-resistant variants from some laboratory-adapted and clinical isolates of HIV-1. However, high-level resistance to maraviroc was selected from three of six primary isolates passaged in peripheral blood lymphocytes (PBL). The SF162 strain acquired resistance to maraviroc in both treated and control cultures; all resistant variants were able to use CXCR4 as a coreceptor. In contrast, maraviroc-resistant virus derived from isolates CC1/85 and RU570 remained CCR5 tropic, as evidenced by susceptibility to the CCR5 antagonist SCH-C, resistance to the CXCR4 antagonist AMD3100, and an inability to replicate in CCR5 Δ32/Δ32 PBL. Strain-specific mutations were identified in the V3 loop of maraviroc-resistant CC1/85 and RU570. The envelope-encoding region of maraviroc-resistant CC1/85 was inserted into an NL4-3 background. This recombinant virus was completely resistant to maraviroc but retained susceptibility to aplaviroc. Reverse mutation of gp120 residues 316 and 323 in the V3 loop (numbering from HXB2) to their original sequence restored wild-type susceptibility to maraviroc, while reversion of either mutation resulted in a partially sensitive virus with reduced maximal inhibition (plateau). The plateaus are consistent with the virus having acquired the ability to utilize maraviroc-bound receptor for entry. This hypothesis was further corroborated by the observation that a high concentration of maraviroc blocks the activity of aplaviroc against maraviroc-resistant virus.
Phase III trial of doxorubicin plus cyclophosphamide (AC), docetaxel, and alternating AC and docetaxel as front-line chemotherapy for metastatic breast cancer: Japan Clinical Oncology Group trial (JCOG9802).

BACKGROUND This randomized, multicenter, phase III trial compared doxorubicin plus cyclophosphamide (AC), single-agent docetaxel (D), and an alternating regimen of AC and docetaxel (AC-D) as first-line chemotherapy in metastatic breast cancer (MBC). PATIENTS AND METHODS Patients with MBC resistant to endocrine therapy were entered in a randomized study to receive either six cycles of AC (doxorubicin 40 mg/m2 plus cyclophosphamide 500 mg/m2), D (60 mg/m2), or alternating treatment with AC-D (i.e. three cycles of AC and three cycles of D). Treatment was administered every 3 weeks. RESULTS A total of 441 patients were entered in a randomized study. Response rates were 30% for AC, 41% for D, and 35% for AC-D. The median times to treatment failure (TTFs) were 6.4, 6.4, and 6.7 months (one-sided log-rank test, P = 0.13 for AC versus D, P = 0.14 for AC versus AC-D) and median overall survival (OS) was 22.6, 25.7, and 25.0 months (P = 0.09 for AC versus D, P = 0.13 for AC versus AC-D) in the AC, D, and AC-D, respectively. CONCLUSION There was no difference in the TTF among the three arms. However, there was a trend toward a better response and better OS in the D than in the AC.
Disruption of Escherichia coli HepA, an RNA Polymerase-associated Protein, Causes UV Sensitivity*

During the development of purification procedures for Escherichia coli RNA polymerase (RNAP), we noticed the consistent co-purification of a 110-kDa polypeptide. Here, we report the identification of the 110-kDa protein as the product of thehepA gene, a member of the SNF2 family of putative helicases. We have cloned the hepA gene and overexpressed and purified the HepA protein. We show in vitro that RNAP preparations have an ATPase activity only in the presence of HepA and that HepA binds core RNAP competitively with the promoter specificity ς70 subunit with a 1:1 stoichiometry and a dissociation constant (K d ) of 75 nm. An E. coli strain with a disruption in the hepA gene shows sensitivity to ultraviolet light.