Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhaled Immunotherapy Administration for Lung Cancer; Efficient? Certainly Possible

Lung cancer is still diagnosed at a late stage in most lung cancer patients. Regarding Non-small Cell lung cancer there are novel therapies such as; tyrosine kinase inhibitors and immunotherapy. Currently we have two immunotherapies that can be used either as first-line treatment or second line treatment; pembrolizumab and nivolumab. A third one is being investigated as a combination of immunotherapy; ipilimumab. Aerosol treatment has been investigated for many diseases not only for the lung, but also for systematic diseases. The design of cups was found the most significant factor in producing significant effects. The comparison of cups reveals the design J as the most capable of reducing the droplets at a minimum size of mass median aerodynamic diameter (MMAD) MMAD=1.99. Drug effect comes second in sequence (F=62.04) showing that nivolumab is the most drastic preparation at low particle sizes (1.89), two drugs share an intermediate particle diameter (pembrolizumab and ipilimumab). In total drugs demonstrate a decreasing droplet size: Ipilimumab>Pembrolizumab> Nivolumab.
Development of bioelectrical impedance analysis prediction equations for body composition with the use of a multicomponent model for use in epidemiologic surveys.

BACKGROUND Previous studies to develop and validate bioelectrical impedance analysis (BIA) equations to predict body composition were limited by small sample sizes, sex specificity, and reliance on reference methods that use a 2-component model. OBJECTIVE This study was designed to develop sex-specific BIA equations to predict total body water (TBW) and fat-free mass (FFM) with the use of a multicomponent model for children and adults. DESIGN Data from 5 centers were pooled to create a sample of 1474 whites and 355 blacks aged 12-94 y. TBW was measured by dilution, and FFM was estimated with a multicomponent model based on densitometry, isotope dilution, and dual-energy X-ray absorptiometry. RESULTS The final race-combined TBW prediction equations included stature(2)/resistance and body weight (R(2) = 0.84 and 0.79 and root mean square errors of 3.8 and 2.6 L for males and females, respectively; CV: 8%) and tended to underpredict TBW in black males (2.0 L) and females (1.4 L) and to overpredict TBW in white males (0.5 L) and females (0.3 L). The race-combined FFM prediction equations contained the same independent variables (R(2) = 0.90 and 0.83 and root mean square errors of 3.9 and 2.9 kg for males and females, respectively; CV: approximately 6%) and tended to underpredict FFM in black males (2.1 kg) and females (1.6 kg) and to overpredict FFM in white males (0.4 kg) and females (0.3 kg). CONCLUSION These equations have excellent precision and are recommended for use in epidemiologic studies to describe normal levels of body composition.
Using fetal cells for prenatal diagnosis: History and recent progress

The potential to use fetal cells in the mother's circulation during the first or second trimester for prenatal diagnosis was described in 1968, but it has not been possible do develop a routine clinical prenatal test despite extensive commercial and academic research efforts. Early attention focused on the detection of aneuploidy, but more recent technology opens the possibility of high resolution detection of copy number abnormalities and even whole genome or exome sequencing to detect both inherited and de novo mutations. In the interim, cell‐free noninvasive prenatal testing NIPT has allowed improved detection of aneuploidy, but this has led to a sharp reduction in the number of amniocentesis and chorionic villus sampling (CVS) procedures, which inevitably implies reduced detection of serious de novo deletion abnormalities. Attention has focused of both fetal nucleated red blood cells (fnRBCs) and trophoblasts. Recent progress presented at meetings, but not yet published, suggests that it will soon be possible to perform genome‐wide relatively high resolution detection of deletions and duplications by recovering fetal trophoblasts during the first trimester and analyzing them by whole gene genome amplification followed by copy number analysis using arrays or next generation sequencing. © 2016 Wiley Periodicals, Inc.
Laser micro sintering of ceramic materials, part 1

Laser Micro Sintering, a generative freeform fabrication technology, was developed as a modification of Selective Laser Sintering in 2002. It owes its unique features mainly to the low grain size of the powder feedstock and a special laser regime. Soon after the first successes with metal powder, endeavours were made to apply the technique onto ceramic materials. However, due to the differences in structure and electronic configuration, the observed principles of laser-material interaction can be transferred from metal to ceramics only to a very limited extent. Results with alumina/silica, SiC and SiO as respective representatives of oxide, non-oxide and sub-oxide ceramic feedstocks are presented, and hypothesized process mechanisms are explained.
Neighborhood Physical Environment and Changes in Body Mass Index: Results From the Multi-Ethnic Study of Atherosclerosis

Longitudinal associations between neighborhood characteristics and body mass index (BMI; weight (kg)/height (m)2) were assessed from 2000 to 2011 among 5,919 participants in the Multi-Ethnic Study of Atherosclerosis. The perceived availability of healthy food and walking environment were assessed via surveys, and 1-mile (1.6-km) densities of supermarkets, fruit-and-vegetable stores, and recreational facilities were obtained through a commercial database. Econometric fixed-effects models were used to estimate the association between within-person changes in neighborhood characteristics and within-person change in BMI. In fully adjusted models, a 1-standard-deviation increase in the healthy food environment index was associated with a 0.16-kg/m2 decrease in BMI (95% confidence interval (CI): -0.27, -0.06) among participants with obesity at baseline. A 1-standard-deviation increase in the physical activity environment index was associated with 0.13-kg/m2 (95% CI: -0.24, -0.02) and 0.14-kg/m2 (95% CI: -0.27, -0.01) decreases in BMI for participants who were overweight and obese at baseline, respectively. Paradoxically, increases in the physical activity index were associated with BMI increases in persons who were normal-weight at baseline. This study provides preliminary longitudinal evidence that favorable changes in neighborhood physical environments are related to BMI reductions in obese persons, who comprise a substantial proportion of the US population.
Comparison of Bonding Performance for CFRP-SPCC Single Lap Joint Using Adhesives and Rivets

The recent trend in automotive industry is characterized by the replacement of existing metal materials with composite ones or the combination of both for lightweight parts. This study 1) created single lap joint specimens of SPCC used for automobile frame and four adhesives; epoxy, urethane, acrylic, mixed (urethane and acrylic) and rivets to bind dissimilar materials of CFRP necessary for weight lightening, and 2) performed a tensile shear test on adhesive bonding versus adhesive bonding with rivets. In summary, this study investigated on the bonding performance of different specimens: bonding strength, shapes of the failure surface, and the effect of rivets on bond strength.
The Impact of the ACA Medicaid Expansions on Health Insurance Coverage through 2015 and Coverage Disparities by Age, Race/Ethnicity, and Gender

OBJECTIVE Examine the ACA Medicaid expansion effects on Medicaid take-up and private coverage through 2015 and coverage disparities by age, race/ethnicity, and gender. DATA SOURCES 2011-2015 American Community Survey for 3,137,989 low-educated adults aged 19-64 years. STUDY DESIGN Difference-in-differences regressions accounting for national coverage trends and state fixed effects. PRINCIPAL FINDINGS Expansion effects doubled in 2015 among low-educated adults, with a nearly 8 percentage-point increase in Medicaid take-up and 6 percentage-point decline in uninsured rate. Significant coverage gains were observed across virtually all examined groups by age, gender, and race/ethnicity. Take-up and insurance declines were strongest among younger adults and were generally close by gender and race/ethnicity. Despite the increased take-up however, coverage disparities remained sizeable, especially for young adults and Hispanics who had declining but still high uninsured rates in 2015. There was some evidence of private coverage crowd-out in certain subgroups, particularly among young adults aged 19-26 years and women, including in both individually purchased and employer-sponsored coverage. CONCLUSIONS The ACA Medicaid expansions have continued to increase coverage in 2015 across the entire population of low-educated adults and have reduced age disparities in coverage. However, there is still a need for interventions that target eligible young and Hispanic adults.
Exploring Professional Help-Seeking for Mental Disorders

Only a third of people with mental disorders seek professional help. In this study, we aimed to investigate attitudes toward help-seeking among non-help-seekers drawn from a community survey in South London. We conducted qualitative interviews with 24 participants with symptoms of mental disorders. We analyzed data using thematic analysis. Participants’ reasons for not seeking professional help included perceiving their problems as normal or unsuitable for professional help, negative expectations of professional help, believing informal strategies were sufficient, fearing being stigmatized for having a mental disorder and help-seeking, and self-perceptions of being strong and/or self-reliant. A small number (n = 4) had resolved their problems. Participants with unresolved mental disorders (n = 20) had more chronic stressors, particularly about finances and family relationships, and greater concerns about professional help and stigma than other participants. Potential targets for interventions to encourage help-seeking could be focused around stigma about help-seeking as well as the self-perception of being strong.
New insights on geographical/ecological populations within Coilia nasus (Clupeiformes: Engraulidae) based on mitochondrial DNA and microsatellites

Abstract The Coilia nasus is an important fish species, which is of commercial values in China. In order to manage the fisheries resources and the development of sustainable fishing strategies to protect this species, 11 microsatellite loci polymorphisms and mtDNA COI sequences were used to examine the genetic diversity of C. nasus in Japan and China. In total, the 40 COI haplotypes and 93 microsatellite alleles were detected. The mtDNA phylogeny did not support population grouping, but the distribution patterns of mtDNA haplotypes and the results of STRUCTURE analysis based on microsatellite indicated a degree of genetic isolation in this species. Our study suggested that the lack of a population genetic structure might result in its amphidromous life cycle, and the geographical distance and habitat fragments might cause isolated populations. Thus, the sampling populations of C. nasus in Japan and China could be divided as four geographical/ecological populations.
Deep RNA profiling identified CLOCK and molecular clock genes as pathophysiological signatures in collagen VI myopathy

ABSTRACT Collagen VI myopathies are genetic disorders caused by mutations in collagen 6 A1, A2 and A3 genes, ranging from the severe Ullrich congenital muscular dystrophy to the milder Bethlem myopathy, which is recapitulated by collagen-VI-null (Col6a1−/−) mice. Abnormalities in mitochondria and autophagic pathway have been proposed as pathogenic causes of collagen VI myopathies, but the link between collagen VI defects and these metabolic circuits remains unknown. To unravel the expression profiling perturbation in muscles with collagen VI myopathies, we performed a deep RNA profiling in both Col6a1−/− mice and patients with collagen VI pathology. The interactome map identified common pathways suggesting a previously undetected connection between circadian genes and collagen VI pathology. Intriguingly, Bmal1−/− (also known as Arntl) mice, a well-characterized model displaying arrhythmic circadian rhythms, showed profound deregulation of the collagen VI pathway and of autophagy-related genes. The involvement of circadian rhythms in collagen VI myopathies is new and links autophagy and mitochondrial abnormalities. It also opens new avenues for therapies of hereditary myopathies to modulate the molecular clock or potential gene–environment interactions that might modify muscle damage pathogenesis. Summary: A new association between hereditary myopathy and CLOCK circuit genes opens novel avenues of research on circadian genes and their role in skeletal muscle hereditary pathologies.
Error Methods Are More Practical, But Uncertainty Methods May Still Be Preferred.

“ Error methods—compared with uncertainty methods—offer simpler, more intuitive and practical procedures for calculating measurement uncertainty and conducting quality assurance in laboratory medicine. However, uncertainty methods are preferred in other fields of sciences as reflected by the guide to the expression of uncertainty in measurement. ” (1). Those are the opening sentences in the report from the Total Error Task and Finish Group (TE-TFG)2 of the European Federation of Clinical Chemistry and Laboratory Medicine (1). The TE-TFG was appointed by the European Federation of Clinical Chemistry and Laboratory Medicine as 1 of the outcomes from the 2014 Milan conference on quality specifications (2) to resolve issues regarding total analytical error (TAE) vs measurement uncertainty (MU). The focus of the 2014 Milan meeting was to review existing recommendations for setting analytical performance specifications and to update the guidelines from the 1999 Stockholm Consensus Conference. One outcome was that the hierarchy of goal-setting models was reduced from 5 to 3 classes—specific models for clinical use, general models related to individual and population variation, and “state-of-the-art” recommendations from expert groups and proficiency testing (PT) and external quality assessment (EQA) programs. Although the PT/EQA criteria for acceptable performance fall into the lowest class, these survey programs are well established globally and require goals for TAE because only a single measurement is allowed on survey samples and that measurement is subject to both random and systematic errors, i.e., precision and trueness. Thus, there is an ongoing need for the TAE model and goals for TAE. TAE vs MU became an issue when ISO 15189 was published in 2007 and required that the “uncertainty of results” be considered in the accreditation of a medical laboratory (3). Another ISO document, Guide to Expression of Uncertainty in Measurement (GUM), became the obvious source for satisfying this …
Withdrawal of red meat from the usual diet reduces albuminuria and improves serum fatty acid profile in type 2 diabetes patients with macroalbuminuria.

BACKGROUND Replacement of red meat in the diet with chicken has reduced the urinary albumin excretion rate (UAER) and serum cholesterol in microalbuminuric type 2 diabetes patients. The effects of withdrawing red meat are unknown in the more advanced stages of diabetic nephropathy. OBJECTIVE Our objective was to assess the effects of replacing red meat in the usual diet (UD) with chicken (CD) and of consuming a lactovegetarian low-protein diet (LPD) on renal function, fatty acid, and lipid profile in macroalbuminuric type 2 diabetes patients. DESIGN A crossover controlled trial was conducted in 17 type 2 diabetes patients with macroalbuminuria (24-h UAER > or = 200 microg/min). Each patient followed the UD, CD, and LPD in a random order for 4 wk. After each diet, glomerular filtration rate, UAER, serum fatty acid, lipid profile, glycemic control, anthropometric indexes, and blood pressure were measured. RESULTS UAER [median CD: 269.4 (range: 111-1128) microg/min; LPD: 229.3 (76.6-999.3) microg/min; UD: 312.8 (223.7-1223.7) microg/min; P < 0.01] and mean (+/-SD) non-HDL cholesterol (CD: 3.92 +/- 0.99 mmol/L; LPD: 3.92 +/- 0.93 mmol/L; UD: 4.23 +/- 1.06 mmol/L; P = 0.042) were lower after CD and LPD than after UD. Compared with the UD, an increase in serum total polyunsaturated fatty acids was also observed (CD: 39.8 +/- 2.6%; LPD: 39.7 +/- 4.4%; UD: 37.3 +/- 3.1%; P = 0.029). CONCLUSION In macroalbuminuric patients with type 2 diabetes, withdrawing red meat from the diet reduces the UAER.
Black tea prevents cigarette smoke-induced oxidative damage of proteins in guinea pigs.

Cigarette smoke (CS) causes oxidative damage and tea polyphenols have strong antioxidant properties. Therefore, we studied the effect of a black tea (BT) infusion on CS-induced oxidative damage of proteins both in vitro and in vivo. In the in vitro experiment, bovine serum albumin (BSA) or a guinea pig tissue microsomal suspension was incubated with an aqueous extract of CS (CS-solution) in the presence or absence of the BT infusion. Protein oxidation was measured by immunoblotting of the dinitrophenylhydrazone derivatives of the protein carbonyls followed by densitometric scanning. Protein degradation was assessed by SDS-PAGE. BT prevented (P < 0.05) CS-induced oxidation of BSA and oxidative degradation of guinea pig lung, liver and heart microsomal proteins. This was also observed when the BT infusion was replaced by its components, i.e, flavonols, theaflavins, thearubigins and catechins. BT prevented microsomal protein degradation by inhibiting oxidative modification of the proteins. The antioxidant effect of BT was similar to that of green tea. In the in vivo experiment, partially ascorbate-deficient guinea pigs were subjected to CS exposure from 5 cigarettes/(guinea pig. d) for 7 d and given water or the BT infusion (20 g/L) to drink. Guinea pigs exposed to CS and given water had extensive oxidation accompanied by 39, 40 and 30% losses (P < 0.05) of microsomal proteins of lung, liver and heart, respectively. However, when the CS-exposed guinea pigs consumed the BT infusion instead of water, the oxidation of microsomal proteins was reduced (P < 0.05) approximately 90, 97 and 70% in lung, liver and heart, respectively. Protein loss was reduced (P < 0.05) approximately 92, 98 and 90% in lung, liver and heart, respectively. The results, if extrapolated to humans, would indicate that regular intake of tea may protect smokers from CS-induced oxidative damage and consequent degenerative diseases.
Morphological variation on isolated populations of Praocis ( Praocis ) spinolai

Abstract In this study, the morphological variations of four geographically isolated populations of Praocis (Praocis) spinolai Gay & Solier (Coleoptera: Tenebrionidae) in the transitional coastal desert, Chile, were studied. The study was conducted in the coastal area of Punta de Choros and Los Choros-Archipelago, which includes three islands: Choros, Damas, and Gaviota. 113 specimens of the species P. (P.) spinolai belonging to the four locations sampled were collected analyzed with geometric morphometrics techniques to explore the pattern of shape variation on the different isolated environments. The principal component analysis revealed a well-defined pattern of variation between the populations analyzed. Moreover, differences between populations emerged also from the canonical variation analysis and were confirmed by the Procrustes ANOVA. All analyses performed confirmed the existence of a pattern of variation, due to the isolation of the populations and to environmental effects. The islands are subject to more arid pressures than the continent, where there is a more stable environment and the presence of coastal wetlands and the coastal range of mountains act together and enable fog condensation. This study indicates the existence of a clear pattern of variation, which indicates an evolutionary trend among the population examined.
High‐throughput interpretation of gene structure changes in human and nonhuman resequencing data, using ACE

Motivation: The accurate interpretation of genetic variants is critical for characterizing genotype‐phenotype associations. Because the effects of genetic variants can depend strongly on their local genomic context, accurate genome annotations are essential. Furthermore, as some variants have the potential to disrupt or alter gene structure, variant interpretation efforts stand to gain from the use of individualized annotations that account for differences in gene structure between individuals or strains. Results : We describe a suite of software tools for identifying possible functional changes in gene structure that may result from sequence variants. ACE (‘Assessing Changes to Exons’) converts phased genotype calls to a collection of explicit haplotype sequences, maps transcript annotations onto them, detects gene‐structure changes and their possible repercussions, and identifies several classes of possible loss of function. Novel transcripts predicted by ACE are commonly supported by spliced RNA‐seq reads, and can be used to improve read alignment and transcript quantification when an individual‐specific genome sequence is available. Using publicly available RNA‐seq data, we show that ACE predictions confirm earlier results regarding the quantitative effects of nonsense‐mediated decay, and we show that predicted loss‐of‐function events are highly concordant with patterns of intolerance to mutations across the human population. ACE can be readily applied to diverse species including animals and plants, making it a broadly useful tool for use in eukaryotic population‐based resequencing projects, particularly for assessing the joint impact of all variants at a locus. Availability and Implementation: ACE is written in open‐source C ++ and Perl and is available from geneprediction.org/ACE Contact: myandell@genetics.utah. edu or tim.reddy@duke.edu Supplementary information: Supplementary information is available at Bioinformatics online.
Why and How to Avoid the Flipped Quaternion Multiplication

Over the last decades quaternions have become a crucial and very successful tool for attitude representation in robotics and aerospace. However, there is a major problem that is continuously causing trouble in practice when it comes to exchanging formulas or implementations: there are two quaternion multiplications in common use, Hamilton's original multiplication and its flipped version, which is often associated with NASA's Jet Propulsion Laboratory. We believe that this particular issue is completely avoidable and only exists today due to a lack of understanding. This paper explains the underlying problem for the popular passive world to body usage of rotation quaternions, and derives an alternative solution compatible with Hamilton's multiplication. Furthermore, it argues for entirely discontinuing the flipped multiplication. Additionally, it provides recipes for efficiently detecting relevant conventions and migrating formulas or algorithms between them.