Miracle-dz/UniFAR-FTUs huggingface dataset:
The dual topoisomerase inhibitor A35 preferentially and specially targets topoisomerase 2α by enhancing pre-strand and post-strand cleavage and inhibiting DNA religation

DNA topoisomerases play a key role in tumor proliferation. Chemotherapeutics targeting topoisomerases have been widely used in clinical oncology, but resistance and side effects, particularly cardiotoxicity, usually limit their application. Clinical data show that a decrease in topoisomerase (top) levels is the primary factor responsible for resistance, but in cells there is compensatory effect between the levels of top1 and top2α. Here, we validated cyclizing-berberine A35, which is a dual top inhibitor and preferentially targets top2α. The impact on the top2α catalytic cycle indicated that A35 could intercalate into DNA but did not interfere with DNA-top binding and top2α ATPase activity. A35 could facilitate DNA-top2α cleavage complex formation by enhancing pre-strand and post-strand cleavage and inhibiting religation, suggesting this compound can be a topoisomerase poison and had a district mechanism from other topoisomerase inhibitors. TARDIS and comet assays showed that A35 could induce cell DNA breakage and DNA-top complexes but had no effect on the cardiac toxicity inducer top2β. Silencing top1 augmented DNA break and silencing top2α decreased DNA break. Further validation in H9c2 cardiac cells showed A35 did not disturb cell proliferation and mitochondrial membrane potency. Additionally, an assay with nude mice further demonstrated A35 did not damage the heart. Our work identifies A35 as a novel skeleton compound dually inhibits topoisomerases, and predominantly and specially targets top2α by interfering with all cleavage steps and its no cardiac toxicity was verified by cardiac cells and mice heart. A35 could be a novel and effective targeting topoisomerase agent.
Notch filters with novel microstrip, triangle-type resonators

A novel notch filter is designed and fabricated using novel microstrip, triangle-type resonators. The main advantage of the new notch filter is superior microwave characteristics in increasing the frequency of the third harmonic response, as demonstrated in simulations using fullwave electromagnetic (EM) simulators. The novel notch filter is demonstrated using the following frequency characteristics: central frequency f/sub 0/ = 1.975 GHz, a 3 dB bandwidth of 39.5%, and an insertion loss S/sub 21/ of -54.273 dB. The experimental and simulation results closely correspond to each other. The superior features of the proposed filter make it suitable for use in microwave communication systems and other applications.
Activating Networks: A Progress Report

Active networks-networks you can add programs to or customize to particular applications-are probably most familiar as Web proxy caches and firewalls. In their more sophisticated form, however, they have become customized infrastructures that let designers program control planes-the control software and network hardware used to manipulate the transport system's behavior. Programming mechanisms such as open signaling are becoming more widespread, for example. But research in active networks has taken a step beyond even these sophisticated infrastructures. Working systems now let designers modify packet-switching infrastructures on the fly using either a switch-like model, which mixes active packets with other packets, or in a capsule model, which regards all packets as programs. In surveying working systems and experimental results, the authors have found that first-generation systems have opted to use modern programming language technologies such as Caml and Java to provide usability and safety, and cryptographic techniques to provide security. The systems differ in the degree of flexibility and performance they offer. Many differences stem from the use of a particular security model. The paper discusses a general architecture for active networks.
Collective construction of dynamics structure: collaboration between semi-active blocks and simple robots

This paper describes a methodology to construct a dynamic structure by the interaction between intelligent blocks and simple robots. The block is intelligent enough to have a rule set and a counter value, and communicates with next blocks based on the rule set and counter value. The structure is formed by growing the chain of the blocks. Its growth direction is determined by the rule set and the counter value. Each robot just loads or unloads the block based on a simple algorithm. The blocks in this method are always attached or detached randomly from the structure. Dynamic structure, in which the local position is unstable but the global structure is stable, has a potential to be fault-tolerant or to be adaptive for environmental changes. In this paper, we describe the mechanism of our proposal and show some fundamental characteristics obtained by computer simulation.
Occupational Transaction after Stroke Constructed as Threat and Balance

Older adults who have had a stroke may experience anxiety, depression and difficulties participating in meaningful occupations while also experiencing excitement, discovery and satisfaction in creating a new occupational balance. The aim of this qualitative study was to explore how older adults experienced the changes in their everyday occupations after a stroke. Five women and three men who had experienced a mild to moderate stroke participated in focus group discussions. Systematic text condensation was applied. The participants' experiences revealed how the stroke was perceived as an ‘occupational threat’ that produced feelings of social exclusion which were experienced as occupational exclusion, deprivation, marginalisation and imbalance. However, at the same time, the participants reconstructed occupational balance by performing occupations in new ways. The participants' experiences provided insight into how they perceived their occupations as threatened after their stroke, while at the same time endeavoring to reconstruct occupational balance. These findings indicate that everyday life after stroke is a time of transaction where people are actively involved in creating control, occupational balance and being socially included.
Imaging direct, dynamin-dependent recapture of fusing secretory granules on plasma membrane lawns from PC12 cells

During exocytosis, secretory granules fuse with the plasma membrane and discharge their content into the extracellular space. The exocytosed membrane is then reinternalized in a coordinated fashion. A role of clathrin-coated vesicles in this process is well established, whereas the involvement of a direct retrieval mechanism (often called kiss and run) is still debated. Here we report that a significant population of docked secretory granules in the neuroendocrine cell line PC12 fuses with the plasma membrane, takes up fluid-phase markers, and is retrieved at the same position. Fusion allows for complete discharge of small molecules, whereas GFP-labeled neuropeptide Y (molecular mass ≈35 kDa) is only partially released. Retrieved granules were preferentially associated with dynamin. Furthermore, recapture is inhibited by guanosine 5′-[γ-thio]triphosphate and peptides known to block dynamin function. We conclude that secretory granules can be recaptured immediately after formation of an exocytotic opening by an endocytic reaction that is spatially and temporally coupled to soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-dependent fusion, but is not a reversal of the fusion reaction.
Rigid-unit phonon modes and structural phase transitions in framework silicates

Abstract The rigid-unit mode model provides many new insights into the stability and physical properties of framework silicates. In this model the SiO4 and AlO4 tetrahedra are treated as very stiff, to a first approximation as completely rigid, in comparison with intertetrahedral forces. In this paper we apply the model to several important examples. The model is reviewed by a detailed study of quartz, and it is shown that the α-β phase transition involves a rigid-unit mode that preserves the Si-O-Si bond angle. The model is used to explain the phase transitions in cristobalite and the different feldspar, sodalite, and leucite structures. We also use the model to explain the nature of the high-temperature disordered phases of cristobalite and tridymite, to interpret the observations of streaks of diffuse scattering in electron diffraction patterns, to interpret the structures in the kalsilite-nepheline solid solution, to explain volume anomalies in the cubic leucite structures, and to explain qualitatively the negative linear thermal expansion in cordierite. The results for the highest symmetry sodalite structure show that there is a rigid-unit mode at every wave vector, a finding with significant implications for the understanding of the sorption and catalytic behavior of zeolites.
Cobra: Content-based Filtering and Aggregation of Blogs and RSS Feeds

Blogs and RSS feeds are becoming increasingly popular. The blogging site LiveJournal has over 11 million user accounts, and according to one report, over 1.6 million postings are made to blogs every day. The "Blogosphere" is a new hotbed of Internet-based media that represents a shift from mostly static content to dynamic, continuously-updated discussions. The problem is that finding and tracking blogs with interesting content is an extremely cumbersome process. In this paper, we present Cobra (Content-Based RSS Aggregator), a system that crawls, filters, and aggregates vast numbers of RSS feeds, delivering to each user a personalized feed based on their interests. Cobra consists of a three-tiered network of crawlers that scan web feeds, filters that match crawled articles to user subscriptions, and reflectors that provide recently-matching articles on each subscription as an RSS feed, which can be browsed using a standard RSS reader. We present the design, implementation, and evaluation of Cobra in three settings: a dedicated cluster, the Emulab testbed, and on PlanetLab. We present a detailed performance study of the Cobra system, demonstrating that the system is able to scale well to support a large number of source feeds and users; that the mean update detection latency is low (bounded by the crawler rate); and that an offline service provisioning step combined with several performance optimizations are effective at reducing memory usage and network load.
LREP: A Language Repository Exchange Protocol

The recent increase in the number and complexity of the language resources available on the Internet is followed by a similar increase of available tools for linguistic analysis. Ideally the user does not need to be confronted with the question in how to match tools with resources. If resource repositories and tool repositories offer adequate metadata information and a suitable exchange protocol is developed this matching process could be performed (semi-) automatically.
The Impact of Arizona's Mandatory Drug Treatment Law on Prosecutorial Plea Bargaining in Drug Cases

Passage of mandatory drug treatment laws continues, but research has yet to examine whether imprisoned low-level offenders (e.g., possession, use, and paraphernalia offenders) may be the product of negotiated plea arrangements. We examine the extent to which prosecutors adjust drug offense seriousness through plea bargaining practices in response to the passage of Arizona's mandatory drug treatment law. A sample of incarcerated drug offenders in Arizona was constructed and data were collected from prosecution and sentencing case files in four counties. Findings show plea bargaining practices play a significant role in the classification and imprisonment of low-level drug cases. Incarcerated marijuana offenders were not first- or second-time offenders, nor were they treated more harshly than other drug offenders. Imprisoned low-level offenders have serious and extensive criminal histories, yet the effect of prior record in plea bargaining outcomes is different pre- and post-mandatory drug treatment. Policy implications regarding plea bargaining practices of drug offenders are discussed.
Characterization and Comparison of Cloud versus Grid Workloads

A new era of Cloud Computing has emerged, but the characteristics of Cloud load in data centers is not perfectly clear. Yet this characterization is critical for the design of novel Cloud job and resource management systems. In this paper, we comprehensively characterize the job/task load and host load in a real-world production data center at Google Inc. We use a detailed trace of over 25 million tasks across over 12,500 hosts. We study the differences between a Google data center and other Grid/HPC systems, from the perspective of both work load (w.r.t. jobs and tasks) and host load (w.r.t. machines). In particular, we study the job length, job submission frequency, and the resource utilization of jobs in the different systems, and also investigate valuable statistics of machine's maximum load, queue state and relative usage levels, with different job priorities and resource attributes. We find that the Google data center exhibits finer resource allocation with respect to CPU and memory than that of Grid/HPC systems. Google jobs are always submitted with much higher frequency and they are much shorter than Grid jobs. As such, Google host load exhibits higher variance and noise.
Clinical Models And Evaluation Measures In The Study Of Experimental Anxiety

Objectives: the following review aims to present accumulated evidence in the literature around the main human models for induction of anxiety and the measures frequently used for its evaluation. Method: It was made a bibliographic research having as source of research articles listed by Pubmed and Medline databases and research in specific books which have investigated the main human models for experimental anxiety induction and the measurements frequently used for its evaluation. Discussion: Both the psychological and chemical models for anxiety induction present ethical limits and are constituted of fundamental links between basic research and therapeutic tests done with the patients. The psychological and/or physiological measures for evaluating experimentally produced anxiety reflect body changes that express the level of the elicited anxiety. Conclusion: The use of clinical models and evaluation measures in the study of experimental anxiety makes possible, under the scientific research, understanding the neurobiological substrate of anxiety in its pathological manifestation, beyond evaluating drugs with anxyolitic potential.
Filtron: A Learning-Based Anti-Spam Filter

We present Filtron, a prototype anti-spam lter that integrates the main empirical con- clusions of our comprehensive analysis on using machine learning to construct eectiv e personalized anti-spam lters. Filtron is based on the experimental results over several design parameters on four publicly available benchmark corpora. After describing Filtron's architecture, we assess its behavior in real use over a period of seven months. The results are deemed satisfactory, though they can be improved with more elaborate preprocessing and regular re-training.
Immunization of Aotus nancymai with recombinant C terminus of Plasmodium falciparum merozoite surface protein 1 in liposomes and alum adjuvant does not induce protection against a challenge infection

Merozoite surface protein 1 (MSP-1) of Plasmodium falciparum is an antimalarial vaccine candidate. The highly conserved 19-kDa C-terminal processing fragment of MSP-1 (MSP-1(19)) is of particular interest since it contains epitopes recognized by monoclonal antibodies which inhibit the invasion of erythrocytes in vitro. The presence of naturally acquired anti-MSP-1(19) antibodies in individuals exposed to malaria has been correlated with reduced morbidity, and immunization with an equivalent recombinant P. yoelii antigen induces substantial protection against this parasite in mice. We have expressed P. falciparum MSP-1(19) in Escherichia coli as a correctly folded protein and immunized Aotus nancymai monkeys by using the protein incorporated into liposomes and adsorbed to alum. After vaccination, the sera from these animals contained anti-MSP-1(19) antibodies, some of which competed for binding to MSP-1(19) with monoclonal antibodies that inhibit parasite invasion of erythrocytes in vitro. However, after challenge with either a homologous or a heterologous strain of parasite, all animals became parasitemic and required treatment. The immunization did not induce protection in this animal model.
Exploring the meaning of participation in a community art project: A case study on the Seeming project†

In Australia, community art has drawn significant research attention in regard to its potential as a community development strategy. Despite the fact that researchers have presented evidence for the positive developmental outcomes of participation in community art projects, a gap remains in understanding how and why people's participation in a community art project can lead to those positive outcomes. This research explored the meaning of participation in a community art project from the vantage point of the people who experience it. Ten participants were interviewed about their participation in a community art project (The Seeming) held in Bendigo, Australia. Following thematic analyses we identified three themes of how participants viewed their participation in a community arts project. These themes included giving voice to the silenced, creation of social connections and challenging and reproducing stereotypes. Participation means coming together and the findings highlight the potential of community arts projects for promoting the creation of new relationships and new stories about community. However, there are also problematic stories about self and others that were not deconstructed. It is argued that the settings in which different groups join can be meaningfully extended if there is an explicit concern with consciousness raising and deconstruction of normative stories. Copyright © 2010 John Wiley & Sons, Ltd.
Relationship Banking in post Bubble Japan : Co-existence of soft-and hard budget constraint ARIKAWA

The purpose of this paper is to provide an overview of the relationship banking in Japan in the 1990s. We show the increasing dependence on bank borrowing in spite of the deregulation of bond market in the mid 1990s in terms of the debt composition, and we confirm the loan from main-bank also increases among the firms with higher bank borrowing. Then, we examine the effects of these facts on borrowing firm behavior. By estimating the employment adjustment function, we present that main bank did not discipline effectively firms that were required the corporate restructuring, while it encouraged the restructuring of the firm with relatively better performance. This paper is prepared for a chapter of the book, “Corporate Governance in Japan: Institutional Change and Organizational Diversity” edited by Masahiko Aoki, Gregory Jackson and Hideaki Miyajima. Two authors are financially supported by RIETI. Keisuke Nitta, Nao Saito, Fumiaki Kuroki helped us to construct the data on which the present paper based. Yurie Otsu has given us excellent help. An early draft was presented at Hitotsubashi University. Comments from Naoto Abe, Katsuyuki Kubo, Shinichi Hirota, Yupana Wiwattanakantang, and Teranishi Juro have been extremely helpful.
AMTS: Adaptive multi-objective task scheduling strategy in cloud computing

Task scheduling in cloud computing environments is a multi-objective optimization problem, which is NP hard. It is also a challenging problem to find an appropriate trade-off among resource utilization, energy consumption and Quality of Service (QoS) requirements under the changing environment and diverse tasks. Considering both processing time and transmission time, a PSO-based Adaptive Multi-objective Task Scheduling (AMTS) Strategy is proposed in this paper. First, the task scheduling problem is formulated. Then, a task scheduling policy is advanced to get the optimal resource utilization, task completion time, average cost and average energy consumption. In order to maintain the particle diversity, the adaptive acceleration coefficient is adopted. Experimental results show that the improved PSO algorithm can obtain quasi-optimal solutions for the cloud task scheduling problem.
Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator

Simian foamy viruses, members of the spumavirus subfamily of retroviruses, are found in a variety of nonhuman primates and, as yet, remain to be characterized with respect to genetic structure and regulation of viral gene expression. The genome of simian foamy virus type 1 (SFV-1), an isolate from rhesus macaques, has been molecularly cloned, and the role of the viral long terminal repeat (LTR) in transcriptional control has been investigated. The SFV-1 LTR is 1,621 base pairs long, and sequence comparisons with human foamy virus revealed a pattern of clustered homology. A cap site in the LTR was identified by analysis of SFV-1 transcripts in infected cells. Transient expression assays in cell lines representing several species and different cell types showed that the SFV-1 LTR has low basal activity in uninfected cells, whereas LTR-directed expression is greatly increased in cells infected with SFV-1. This transactivation is mediated by a mechanism involving increases in steady-state levels of viral transcripts. Thus, the SFV-1 genome encodes a transactivator that functions on the LTR at the transcriptional level.
Space Efficient Suffix Trees

We first give a representation of a suffix tree that uses \(n \lg n + O(n)\) bits of space and supports searching for a pattern in the given text (from a fixed size alphabet) in O(m) time, where n is the size of the text and m is the size of the pattern. The structure is quite simple and answers a question raised by Muthukrishnan in [17]. Previous compact representations of suffix trees had a higher lower order term in space and had some expectation assumption [3], or required more time for searching [5]. Then, surprisingly, we show that we can even do better, by developing a structure that uses a suffix array (and so \(n \lceil \lg n \rceil \) bits) and an additional o(n) bits. String searching can be done in this structure also in O(m) time. Besides supporting string searching, we can also report the number of occurrences of the pattern in the same time using no additional space. In this case the space occupied by the structures is much less compared to many of the previously known structures to do this. When the size of the alphabet k is not a constant, our structures can be easily extended, using standard tricks, to those that use the same space but take \(O(m \lg k)\) time for string searching or to those that use an additional \(O(m \lg k)\) bits but take the same O(m) time for searching.