Miracle-dz/UniFAR-FTUs huggingface dataset:
Improved Function After Deep Brain Stimulation for Chronic, Severe Traumatic Brain Injury.

BACKGROUND Severe traumatic brain injury (TBI) damages the frontal lobes and connecting networks, which impairs executive functions, including the ability to self-regulate. Despite significant disabling effects, there are few treatment options in the chronic phase after injury. OBJECTIVE To investigate the safety and potential effectiveness of deep brain stimulation (DBS) for individuals with chronic, disabling TBI and problems of behavioral and emotional self-regulation. METHODS This study was an open-label, prospective design with serial assessments of behavioral outcomes and positron emission tomography 2 years after DBS implantation. Four participants 6 to 21 years after severe TBIs from automobile crashes were included. Although alert and volitional, all experienced significant executive impairments, including either impulsivity or reduced initiation. DBS implants were placed bilaterally in the nucleus accumbens and anterior limb of the internal capsule to modulate the prefrontal cortex. RESULTS The procedure was safe, and all participants had improved functional outcomes. Two years after implantation, 3 met a priori criteria for improvement on the Mayo-Portland Adaptability Inventory-4. Improvement was due largely to better emotional adjustment, although 1 participant showed marked increases in multiple domains. Significant improvement in a composite score of functional capacity indicated improved independence in self-care and activities of daily living. The pattern of change in cognition corresponded with changes in activation of the prefrontal cortex observed in serial scanning. CONCLUSION This first study of DBS to this target for severe TBI supports its safety and suggests potential effectiveness to improve function years after injury. The primary impact was on behavioral and emotional adjustment, which in turn improved functional independence. ABBREVIATIONS DBS, deep brain stimulationIC, internal capsuleMPAI-4, Mayo-Portland Adaptability Inventory-4NAcc, nucleus accumbensTBI, traumatic brain injury.
Efficient Hammerhead Ribozymes Targeted to the Polycistronic Sendai Virus P/C mRNA

The Sendai virus polycistronic P/C mRNA encodes the P and C proteins from alternate overlapping reading frames. To determine the functions of these proteins in virus replication, hammerhead ribozymes were targeted to cleave the 5′-untranslated region of the P/C mRNA. Both cell-free and intracellular assays were employed to determine ribozyme efficacy. To appropriately compare activities between cell-free and intracellular assays, identical ribozymes were synthesized in vitro as well as expressed in cells. Ribozyme parameters, namely hybridization arm length (HAL) and nonhybridizing extraneous sequences (NES), were found to have rate-determining properties. In cell-free reactions, ribozymes with 13-mer HAL were up to 10-fold more efficient than those with 9-mer HAL. Ribozymes with 9-mer HAL were relatively ineffective in transfected cells. Minimizing the number of NES increased ribozyme efficiency in vitro. However, ribozymes with minimal NES were essentially inert intracellularly. The NES at the termini of the most effective intracellular ribozyme, Rz13st (∼95% inhibition of the p gene expression), were predicted to fold into stem-loop structures. These structures most likely increase ribozyme stability as evidenced by the 8-fold higher resistance to ribonuclease T2 digestion of Rz13st compared with Rz13B. Our results suggest that when designing effective intracellular ribozymes, parameters that enhance formation of productive ribozyme:substrate duplexes and that increase RNA stability should be optimized.
Molecular Investigation of Virulence Determinants between a Virulent Clinical Strain and an Attenuated Strain of Burkholderia pseudomallei

Burkholderia pseudomallei is the causative agent of melioidosis. We initiated this investigation with a virulent and an attenuated strain of B. pseudomallei. Pulsed-field gel electrophoresis was carried out initially for macrogenomic comparison of both strains of B. pseudomallei. However, the pulsotypes obtained were identical and therefore we applied a subtractive hybridization technique to distinguish and determine the possible differences between the two strains. Six virulence strain-specific DNA fragments were obtained and the encoding homolog proteins were identified as a xenobiotic-responsive element family of transcriptional regulator, a hypothetical protein, an unknown protein, a plasmid recombination enzyme, a regulatory protein and a putative hemolysin activator protein. A combination of at least three of these determinants was identified in 45 clinical isolates when screening was carried out with self-designed multiplex PCR targeting the six putative virulent determinants. Our data demonstrated that different combinations of the six putative virulence genes were present in the clinical isolates indicating their probable role in the pathogenesis of B. pseudomallei infections.
GEPASI: a software package for modelling the dynamics, steady states and control of biochemical and other systems

GEPASI is a software system for modelling chemical and biochemical reaction networks on computers running Microsoft Windows. For any system of up to 45 metabolites and 45 reactions, each with any user-defined or one of 35 predefined rate equations, one can produce trajectories of the metabolite concentrations and obtain a steady state (if it does exist). When steady-state solutions are produced, elasticity and control coefficients, as defined in metabolic control analysis, are calculated. GEPASI also allows the automatic generation of a sequence of simulations with different combinations of parameter values, effectively scanning a hyper-solid in parameter space. Together with the ability to produce user-defined columnar data files, these features allow for both very quick and systematic study of biochemical pathway models. The source code (in C) is available on request from the author, and while the user interface is dependent on having MS-Windows as the operating system, the numerical part is portable to other operating systems. GEPASI is suitable both for research and educational purposes. Although GEPASI was written with biochemical pathways in mind, it can equally be used to stimulate other dynamical systems.
Emerging perceptions and perspectives of Filipino American middle school students

The purpose of this study is to explore (a) issues perceived by Filipino American middle school students in the Los Angeles area of Southern California as having an adverse impact on their educational experience and cultural identity; (b) ways in which students have personally mediated these issues; and (c) student perspectives related to the types of programs and activities believed to have the most positive impact on the educational experience and cultural identity development of Filipino American middle school students. The middle school experience within the Los Angeles area of Southern California has provided Filipino American students with a standard-based curriculum and school-based activities for learning. However, the school curriculum and school-based activities limits opportunities for Filipino American middle school students to deepen their knowledge of Filipino American education experiences and cultural identity development. Therefore, there is a tremendous opportunity to explore the educational experiences and cultural identity development of Filipino American middle school students in Southern California area of Los Angeles. The literature has revealed that Filipino American students who graduate from public schools enter postsecondary education unprepared and academically challenged. Further review of the literature has revealed that little research has been done to determine the issues perceived by Filipino American middle school students, how they mediate issues, and what types of programs and activities have the most positive impact on their educational experience and cultural identity development. This study explored the lived experiences of 16 Filipino American middle school students within the Los Angeles area of Southern California. The top seven topics that
Molecular Characterization of the Tobacco Rattle Virus RNA2 Genome Isolated from Gladiolus

Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3′ terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.
Loan pricing under estimation risk

Abstract Financial product prices often depend on unknown parameters. Their estimation introduces the risk that a better informed counterparty may strategically pick mispriced products. Understanding estimation risk, and how to properly price it, is essential. We discuss how total estimation risk can be minimized by selecting a probability model of appropriate complexity. We show that conditional estimation risk can be measured only if the probability model predictions have little bias. We illustrate how a premium for conditional estimation risk may be determined when one counterparty is better informed than the other, but a market collapse is to be avoided, using a simple example from pricing regime credit scoring. We empirically examine the approach on a panel data set from a German credit bureau, where we also study dynamic dependencies such as prior rating migrations and defaults.
Effects of lexical status and morphological complexity in masked priming: An ERP study

Two masked priming experiments examined behavioural and event-related potential responses to simplex target words (e.g., flex) preceded by briefly presented, masked, derived word primes (flexible-flex), complex nonword primes formed by an illegal combination of the target word and a real suffix (flexify-flex), and simplex nonword primes formed by adding a nonsuffix word ending to the target (flexint-flex). Subjects performed a lexical decision task. Behavioural results showed that all prime types significantly facilitated target recognition. Priming effects were reflected in the electrophysiological data by reduced N250 and N400 amplitudes, and these priming effects were statistically equivalent for the three types of prime. The strong priming effects found with simplex primes in the present study, compared with prior research, are thought to be due to the combination of targets always being completely embedded in prime stimuli plus the reduced lexical inhibition that arises with nonword primes. In line with prior behavioural research, however, there was evidence for differential priming effects as a function of prime type in the N400 ERP component in Experiment 2, with greater priming effects for derived and pseudocomplex primes relative to simplex primes at lateral posterior electrode sites.
Effect of Gender on Sympathovagal Imbalance in Prehypertensives

Although recently the incidence of prehypertension has increased considerably, the pathophysiological mechanisms and the effects of gender in its causation have not yet been fully elucidated. Therefore, in this study body mass index (BMI), waist–hip ratio (WHR), basal heart rate (BHR), blood pressure (BP), rate pressure product (RPP), and spectral indices of heart rate variability (HRV) were reordered and analyzed in normotensive and prehypertensive males and females. It was observed that low frequency–high frequency (LF–HF) ratio, the sensitive indicator of sympathovagal imbalance (SVI), is significantly more (P < .001) in male prehypertensives compared with female prehypertensives. Although SVI in prehypertensives was found to be due to both sympathetic activation and vagal inhibition, contribution of vagal withdrawal was prominent in males. The LF–HF ratio was significantly correlated with BMI, WHR, BHR, BP, and RPP, which was more prominent in male prehypertensives and the degree of correlation was more for WHR and diastolic pressure. It was concluded that vagal inhibition plays an important role in critical alteration of SVI in the genesis of prehypertension, especially in males, and WHR could be a better indicator of SVI in prehypertensives. It was suggested that prehypertensives should improve their vagal tone to restore the sympathovagal homeostasis.
THE USE OF HEART RATE TO ESTIMATE OXYGEN CONSUMPTION OF FREE-RANGING BLACK-BROWED ALBATROSSES DIOMEDEA MELANOPHRYS

Heart rates (fh) and rates of oxygen consumption (V(dot)O2) were measured in eight black-browed albatrosses (Diomedea melanophrys) when walking on a treadmill, with the aim of using fh to predict V(dot)O2 in free-ranging albatrosses. The resulting relationship between the variables was: V(dot)O2 (ml min-1) = [0.0157fh (beats min-1)]1.60, r2=0.80, P<0.001. In addition to the calibration procedure, six of the albatrosses were injected with doubly labelled water (DLW), and fh and V(dot)O2 were monitored continuously over a 3 day period while the birds were held in a respirometer. During the 3 day period, the birds were walked for up to 3­4 h day-1 in bouts lasting approximately 0.5 h. The heart rate data were used to estimate the metabolic rates of these birds using the above regression. Estimates of metabolic rate derived from fh, DLW and respirometry did not differ (ANOVA; P=0.94), primarily because of the variance between individual birds. There was also no significant difference between the different estimates obtained from the different equations used to calculate energy expenditure from the DLW technique (ANOVA; P=0.95). Mean estimates of V(dot)O2 from fh under active and inactive conditions differed from measured values of V(dot)O2 by -5.9 % and -1.7 % respectively. In addition, the estimates of V(dot)O2 from fh at different walking speeds did not differ significantly from the measured values. It appears that, in the black-browed albatross, fh is as good a predictor of the mean metabolic rate of free-ranging birds as DLW or time­energy budgets combined with either respirometry or DLW. However, the method should be applied to as many individuals and as many instances of a particular behaviour as possible. The heart rate technique offers potential for much more detailed analyses of the daily energy budgets of these birds, and over much longer periods, than has previously been possible.
Overexpression of Twinkle-helicase protects cardiomyocytes from genotoxic stress caused by reactive oxygen species

Significance In the present work, we show that overexpression of TWINKLE helicase reduces the amount of ROS-induced mtDNA mutations and ameliorates cardiomyopathy in Sod2+/− mice. We demonstrate that increased ROS in mitochondria result in a rise of base transversions and mtDNA rearrangements. Increased TWINKLE availability improves mtDNA integrity and protects cardiomyocytes by inhibiting apoptosis via p21. Our findings offer unique approaches to limit the loss of cardiomyocytes due to oxidative stress, a common problem in various disease conditions and during normal aging. Mitochondrial DNA (mtDNA) in adult human heart is characterized by complex molecular forms held together by junctional molecules of unknown biological significance. These junctions are not present in mouse hearts and emerge in humans during postnatal development, concomitant with increased demand for oxidative metabolism. To analyze the role of mtDNA organization during oxidative stress in cardiomyocytes, we used a mouse model, which recapitulates the complex mtDNA organization of human hearts by overexpression of the mitochondrial helicase, TWINKLE. Overexpression of TWINKLE rescued the oxidative damage induced replication stalling of mtDNA, reduced mtDNA point mutation load, and modified mtDNA rearrangements in heterozygous mitochondrial superoxide dismutase knockout hearts, as well as ameliorated cardiomyopathy in mice superoxide dismutase knockout in a p21-dependent manner. We conclude that mtDNA integrity influences cell survival and reason that tissue specific modes of mtDNA maintenance represent an adaptation to oxidative stress.
Photoacoustic-Based-Close-Loop Temperature Control for Nanoparticle Hyperthermia

Goal: Hyperthermia therapy requires tight temperature control to achieve selective killing of cancerous tissue with minimal damage on surrounding healthy tissues. Methods: To this end, accurate temperature monitoring and subsequent heating control are critical. However, an economic, portable, and real-time temperature control solution is currently lacking. To bridge this gap, we present a novel portable close-loop system for hyperthermia temperature control, in which photoacoustic technique is proposed for noninvasive real-time temperature measurement. Exploiting the high sensitivity of photoacoustics, the temperature is monitored with an accuracy of around 0.18 °C and then fed back to a controller implemented on field programmable gate array (FPGA) for temperature control. Dubbed as portable hyperthermia feedback controller (pHFC), it stabilizes the temperature at preset values by regulating the hyperthermia power with a proportional-integral-derivative (PID) algorithm; and to facilitate digital implementation, the pHFC further converts the PID output into switching values (0 and 1) with the pulse width modulation (PWM) algorithm. Results: Proof-of-concept hyperthermia experiments demonstrate that the pHFC system is able to bring the temperature from baseline to predetermined value with an accuracy of 0.3° and a negligible temperature overshoot. Conclusion: The pHFC can potentially be translated to clinical applications with customized hyperthermia system design. Significance: This paper can facilitate future efforts in seamless integration of close-loop temperature control solution and various clinical hyperthermia systems.
Reactive power control for load sharing with virtual synchronous generator control

Distributed generators such as photovoltaic, wind turbine and gas cogeneration system have been paid more attention. Distributed generators are often connected to a grid by inverters. Inverters used in distributed generators are generally controlled by a PLL (Phase Locked Loop) in order to be synchronized with power system frequency and phase. The power system will become more unstable if the capacity of inverter-connected type distributed generators becomes larger and larger, because inverter frequency is controlled just to follow the frequency determined by other synchronous generators. The concept of “Virtual Synchronous Generator” (VSG) which is to control inverters to behave like a real synchronous generator has been proposed. VSG has virtual inertia which is realized by a small energy storage device to mimic rotor's inertia and obtain synchronizing power. So far, an active power control scheme of VSG which is based on the swing equation of a synchronous generator has been employed in previous studies. However, a reactive power control method for VSG has not been mentioned. In this paper, a droop control for keeping sending end voltage of inverters constant is proposed. A method for sharing reactive power proportional to their capacity is also presented. Applying these methods to the VSG control inverters, equitable sharing of active and reactive power is realized. These methods are verified through circuit simulation. Moreover, experiments are conducted using 10 kW level inverters and simulated transmission lines.
Budget Theory and Budget Practice: How Good the Fit?.

Theory in budgeting, like much of public administration, has been of two kinds, descriptive and normative. Descriptive theory is based on close observation or participation in public sector activities. Theorists describe trends, sequences of events, and infer causes, paying attention to local variations as well as uniformities across cases. Normative theory-advice-may be based on a much narrower range of observations than descriptive theory and its proposed solutions may be based on values rather than observations. If the explanatory power of the descriptive theory is too weak, or if the advice of normative theory is not adopted by public officials or is adopted and abandoned because it does not work, the gap between theory and practice may become unacceptably wide.
Targeting of heterologous membrane proteins into proliferated internal membranes in Saccharomyces cerevisiae

Overproduction of chimeric proteins containing the HMG2/1 peptide, which comprises the seven transmembrane domains of Saccharomyces cerevisiae 3‐hydroxy‐3‐methylglutaryl‐CoA reductase isozymes 1 and 2, has previously been observed to induce the proliferation of internal endoplasmic reticulum‐like membranes. In order to exploit this amplified membrane surface area for the accommodation of heterologous microsomal proteins, we fused sequences coding for human cytochrome P4501A1 (CYP1A1) to sequence encoding the HMG2/1 peptide and expressed the hybrid genes in yeast. The heterologous hybrid proteins were targeted into strongly proliferated membranes, as shown by electron microscopic and immunofluorescent analysis. Fusion proteins comprising the whole CYP1A1 polypeptide (HMG2/1‐CYP1A1) exhibited 7‐ethoxyresorufin‐O‐deethylase activity, whereas fusion proteins lacking the N‐terminal 56 amino acids of CYP1A1 (HMG2/1‐ΔCYP1A1) were inactive and appeared to be unable to incorporate protoheme. Similar amounts of heterologous protein were detected in cells expressing HMG2/1‐CYP1A1, HMG2/1‐ΔCYP1A1 and CYP1A1, respectively. Replacement of the N‐terminal membrane anchor domain of human NADPH‐cytochrome P450 oxidoreductase by the HMG2/1 peptide also resulted in a functional fusion enzyme, which was able to interact with HMG2/1‐CYP1A1 and the yeast endogenous P450 enzyme lanosterol‐14α‐demethylase.
Evaluating the appropriateness of hospital doctors’ requests for pulmonary function tests beyond basic spirometry: results from a prospective observational study

ABSTRACT Objectives: Hospitalists request ‘complete’ pulmonary function tests (PFTs), typically comprising of spirometry, diffusion capacity of the lung for carbon monoxide (DLCO) and absolute lung volumes (ALVs), the results of which assist in the management of patients with respiratory disorders. Recently, concerns have been raised about over-requesting of ‘complete’ PFTs, but there is a paucity of information on the proportion of requests that can be considered clinically inappropriate. This study prospectively evaluated the ‘complete’ PFTs requested in a hospital service and assessed the impact of medical review of the requests. Methods: A six-month prospective study on requests to two teaching hospital PFT laboratories from non-respiratory doctors was undertaken. Requests at one laboratory underwent review by a respiratory doctor (‘intervention laboratory’) while requests at the second laboratory were not reviewed (‘control laboratory’). The appropriateness of requests was measured against pre-specified criteria. Results: PFT requests for 335 subjects were included in the study. In the intervention laboratory, 8 of 110 ALV and 122 of 134 DLCO requests fulfilled pre-specified criteria for appropriate test indications. Fewer ALV (7% vs. 100%, p < 0.001) and DLCO tests (91% vs. 100%, p = 0.031) could have been performed in the intervention laboratory compared to the control laboratory. Conclusion: A considerable proportion of ‘complete’ PFT requests from non-respiratory hospital doctors may be unwarranted. Using a simple screening method, the number of unnecessary PFTs could be reduced, resulting in substantial time and cost savings for hospital PFT laboratories.