Miracle-dz/UniFAR-FTUs huggingface dataset:
A Novel , Highly Effective and Environmentally Friendly 1 Recombinant Baculovirus Insecticide , NeuroBactrus 2 3 Running title : Novel environment-friendly baculovirus insecticide

31 A novel recombinant baculovirus, NeuroBactrus, was constructed to develop an improved 32 baculovirus insecticide with additional beneficial properties, such as a higher insecticidal activity 33 and improved recovery, compared to wild-type baculovirus. For the construction of the 34 NeuroBactrus, the Bacillus thuringiensis cry1-5 crystal protein gene was introduced into the 35 Autographa californica nucleopolyhedrovirus (AcMNPV) genome by fusion of polyhedrin-cry136 5-polyhedrin under the control of the polyhedrin promoter. In the opposite direction, an insect37 specific neurotoxin gene, AaIT, from Androctonus australis was introduced under the control of 38 an early promoter from Cotesia plutellae bracovirus by fusion of a partial fragment of orf603. 39 The Polyhedrin-Cry1-5-Polyhedrin fusion protein expressed by the NeuroBactrus was not only 40 occluded into the polyhedra, but it was also activated by treatment with trypsin, resulting in an 41 approximately 65-kDa active toxin. In addition, qPCR revealed that the neurotoxin was 42 expressed from the early phase of infection. The NeuroBactrus showed a high level of 43 insecticidal activity against Plutella xylostella larvae and a significant reduction in the median 44 lethal time (LT50) against Spodoptera exigua larvae compared to those of wild-type AcMNPV. 45 Re-recombinant mutants derived from NeuroBactrus in which AaIT and/or cry1-5 were deleted 46 were generated by serial passages in vitro. Expression of the foreign proteins (Bt toxin and AaIT) 47 was continuously reduced during the serial passage of the NeuroBactrus. Moreover, polyhedra 48 collected from S. exigua larvae infected with the serially passed NeuroBactrus showed 49 insecticidal activity similar to that of wild-type AcMNPV. These results suggested that the 50 NeuroBactrus could be recovered to wild-type AcMNPV through serial passaging. 51
Related family-based attribute reduction of covering information systems when varying attribute sets

In practical situations, there are many dynamic covering information systems with variations of attributes, but there are few studies on related family-based attribute reduction of dynamic covering information systems. In this paper, we first investigate updated mechanisms of constructing attribute reducts for consistent and inconsistent covering information systems when varying attribute sets by using related families. Then we employ examples to illustrate how to compute attribute reducts of dynamic covering information systems with variations of attribute sets. Finally, the experimental results illustrates that the related family-based methods are effective to perform attribute reduction of dynamic covering information systems when attribute sets are varying with time.
Control of Periodontal Infection Reduces the Severity of Active Rheumatoid Arthritis

Objective:To determine if eliminating periodontal infection and gingival inflammation affects the severity of active rheumatoid arthritis (RA) in patients with chronic inflammatory periodontal disease. Methods:Twenty-nine subjects with confirmed diagnosis of RA and mild-to-moderate chronic periodontitis of at least 3 years’ duration were enrolled in the study. The activity of RA was assessed using the disease activity score test (DAS28). Seventeen subjects completing the study received periodontal treatment consisting of scaling/root planing and oral hygiene instruction; 12 subjects completing the study received no treatment. Participants continued their usual disease-modifying medications for RA without any changes in DMARD therapy during the study period. RA measurements, and periodontal indices were recorded at baseline and at 8 weeks for each participant. Mann-Whitney U and χ2 tests were used to test for significant differences in the severity of RA in the periodontally treated group compared with the untreated groups. Results:Ten of 17 subjects (58.8%) in the treated group and 2 of 12 subjects (16.7%) in the untreated group showed improvement in RA scores. There was a statistically significant difference in DAS28 (4.3 ± 1.6 vs. 5.1 ± 1.2) and erythrocyte sedimentation rate (31.4 ± 24.3 vs. 42.7 ± 22) between the treatment and the control groups. Conclusion:Control of periodontal infection and gingival inflammation by scaling/root planing and plaque control in subjects with periodontal disease may reduce the severity of RA. This notion is supported by reported subjective improvement in treated patients.
Multiblock PLS analysis of an industrial pharmaceutical process

The performance of an industrial pharmaceutical process (production of an active pharmaceutical ingredient by fermentation, API) was modeled by multiblock partial least squares (MBPLS). The most important process stages are inoculum production and API production fermentation. Thirty batches (runs) were produced according to an experimental planning. Rather than merging all these data into a single block of independent variables (as in ordinary PLS), four data blocks were used separately (manipulated and quality variables for each process stage). With the multiblock approach it was possible to calculate weights and scores for each independent block. It was found that the inoculum quality variables were highly correlated with API production for nominal fermentations. For the nonnominal fermentations, the manipulations of the fermentation stage explained the amount of API obtained (especially the pH and biomass concentration). Based on the above process analysis it was possible to select a smaller set of variables with which a new model was built. The amount of variance predicted of the final API concentration (cross‐validation) for this model was 82.4%. The advantage of the multiblock model over the standard PLS model is that the contributions of the two main process stages to the API volumetric productivity were determined. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 419–427, 2002.
Embodied Deviance, Gender, and Epistemologies of Ignorance: Re-Visioning Drugs Use in a Neurochemical, Unjust World

The paper develops key notions needed for a feminist embodiment approach to drugs, their use and users. First, the term embodied deviance is defined in relationship to women drug users. Second, the bodily tasks of gendered drug use are defined to show how “normal” embodiment is foreclosed to women drug users. Third, disease regimes and epistemologies of ignorance are introduced. Fourth, another piece is inserted into the feminist embodiment puzzle –emotions. Simply, we look at some of the practices that emerge from the affective dimensions of gendered drug use. In the concluding section of my paper, I ask, “Where do we go from here? ”
Estrogen, through estrogen receptor 1, regulates histone modifications and chromatin remodeling during spermatogenesis in adult rats

ABSTRACT Estrogen receptors (ESR1 and ESR2) play crucial roles in various processes during spermatogenesis. To elucidate individual roles of ESRs in male fertility, we developed in vivo selective ESR agonist administration models. Adult male rats treated with ESR1 and ESR2 agonist for 60 days show spermatogenic defects leading to reduced sperm counts and fertility. While studying epigenetic changes in the male germ line that could have affected fertility, we earlier observed a decrease in DNA methylation and its machinery upon ESR2 agonist treatment. Here, we explored the effects on histone modifications, which could contribute to decreased male fertility upon ESR agonist administration. ESR1 agonist treatment affected testicular levels of histone modifications associated with active and repressed chromatin states, along with heterochromatin marks. This was concomitant with deregulation of corresponding histone modifying enzymes in the testis. In addition, there was increased retention of histones along with protamine deficiency in the caudal spermatozoa after ESR1 agonist treatment. This could be due to the observed decrease in several chromatin remodeling proteins implicated in mediating histone-to-protamine exchange during spermiogenesis. The activating and repressing histone marks in spermatozoa, which play a critical role in early embryo development, were deregulated after both the ESR agonist treatments. Together, these epigenetic defects in the male germ line could affect the spermatozoa quality and lead to the observed decrease in fertility. Our results thus highlight the importance of ESRs in regulating different epigenetic processes during spermatogenesis, which are crucial for male fertility.
Optically reconfigurable matching networks

In this paper an optically reconfigurable circuit as a part of the input matching network is proposed and applied in order to design a 2.5-3.5 GHz frequency tunable power amplifier. Tunability is achieved by increasing the power of the laser applied to the highly resistive silicon wafer and changing the properties of silicon under optical illumination. Tunable class AB power amplifier with the optical switch in the input matching circuit has obtained the frequency tuning range of 2.5-3.5 GHz with no significant loss in efficiency and linearity.
Anterior-posterior transcranial ultrasound to measure cranial oscillations.

BACKGROUND We aimed to provide information on whether or not the correlation between body tilt and the pulse amplitude of transcranial ultrasonic time-of-flight waveform can be observed in the anterior-posterior skull direction. Also, we asked the question whether or not the skull pulsation can be detected since the cranial bones involved are thicker. METHODS The experimental model of body tilt that alters intracranial pressure by shifting body fluid headward was employed. Transcranial ultrasound waveforms were examined in 15 healthy volunteers positioned at five tilt angles of +30 degrees, 0 degrees, -30 degrees, -60 degrees, and -90 degrees from the horizontal body position. A pulse-echo transducer was placed on the middle forehead and ultrasound waveforms were recorded. Synchronized variations in the ultrasonic time-of-flight with heartbeats were monitored using the pulsed phase locked loop technique for the output voltage of the ultrasound transducer. Simultaneous effects of body tilt on cardiovascular parameters were also evaluated. RESULTS Pulse amplitudes of ultrasonic time-of-flight waveforms were found to vary with body tilt. Repeated-measures ANOVA and regression analysis showed a negative correlation between body tilt angle and pulse amplitude. The regression line has the equation: pulse amplitude = (1.158-0.01023 x tilt angle) x 10(-4) voltage. There was no such relationship between head-down body tilt and altered mean blood pressure or heart rate. CONCLUSION An increase in the pulse amplitude of the anterior-posterior transcranial ultrasonic time-of-flight waveform can be detected when the head-down body tilt angle increases.
Recombinant Paramyxovirus Type 1-Avian Influenza-H7 Virus as a Vaccine for Protection of Chickens Against Influenza and Newcastle Disease

Abstract Current vaccines to prevent avian influenza rely upon labor-intensive parenteral injection. A more advantageous vaccine would be capable of administration by mass immunization methods such as spray or water vaccination. A recombinant vaccine (rNDV-AIV-H7) was constructed by using a lentogenic paramyxovirus type 1 vector (Newcastle disease virus [NDV] B1 strain) with insertion of the hemagglutinin (HA) gene from avian influenza virus (AIV) A/chicken/NY/13142-5/94 (H7N2). The recombinant virus had stable insertion and expression of the H7 AIV HA gene as evident by detection of HA expression via immunofluorescence in infected Vero cells. The rNDV-AIV-H7 replicated in 9–10 day embryonating chicken eggs and exhibited hemagglutinating activity from both NDV and AI proteins that was inhibited by antisera against both NDV and AIV H7. Groups of 2-week-old white Leghorn chickens were vaccinated with transfectant NDV vector (tNDV), rNDV-AIV-H7, or sterile allantoic fluid and were challenged 2 weeks later with viscerotropic velogenic NDV (vvNDV) or highly pathogenic (HP) AIV. The sham-vaccinated birds were not protected from vvNDV or HP AIV challenge. The transfectant NDV vaccine provided 70% protection for NDV challenge but did not protect against AIV challenge. The rNDV-AIV-H7 vaccine provided partial protection (40%) from vvNDV and HP AIV challenge. The serologic response was examined in chickens that received one or two immunizations of the rNDV-AIV-H7 vaccine. Based on hemagglutination inhibition and enzyme-linked immunosorbent assay (ELISA) tests, chickens that received a vaccine boost seroconverted to AIV H7, but the serologic response was weak in birds that received only one vaccination. This demonstrates the potential for NDV for use as a vaccine vector in expressing AIV proteins.
Ex vivo protocol for testing virus survival on human skin: experiments with herpesvirus 2

We report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. Using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. At the physiological skin temperature of 32 degrees C, the half-life of the virus on skin was 1.44 h while on metal disks it was 0.36 h. Even at ambient temperature (22 degrees C), the virus lost infectivity faster (half-life = 0.96 h) on metal disks than on the skin at 32 degrees C. The method described could be used to assess the survival of other human pathogens on skin and to evaluate the germicidal activity of handwashing agents and other topicals.
A survey on human motion capturing (HMC) system based on distributed computing technology with flexible architecture

The evolution in people and technology over a period of time has resulted in the current day situation where based on the posture of the human being, the probable diseases to which he is prone can be predicted. In present day scenario where applications involving healthcare sectors, military sectors to entertainment sectors, the detection and recognition of human movements and gestures has become more prevalent than before due to its widespread applications. The applicability of posture detection system is realized using the Human Motion Capture (HMC) system. To better understand the features of HMC, In in this paper a general working of a HMC system is given, consecutively, a brief review of literature along with the research methodologies and techniques are provided for each stage constituting the HMC system. The research gap and the future scope of the HMC system is also mentioned with corresponding research challenges.
Optimization of Storage and Stability of Camel Liver Glutathione S-Transferase

Glutathione S-transferases (GSTs) are multifunctional enzymes and play an important role in cellular detoxification. Besides this, GSTs act as cytosolic carrier proteins that bind hydrophobic compounds such as heme, bilirubin, steroids, and polycyclic hydrocarbons. GST has great importance in biotechnology, as it is a target for vaccine and drug development and biosensors development for xenobiotics. Moreover, the GST tag has been extensively used for protein expression and purification. Until now, biophysical properties of camel liver GST have not been characterized. In the present study we have purified camel (Camelus dromedarius) liver GST to homogeneity in a single step by affinity chromatography with 23.4-fold purification and 60.6% yield. Our results showed that maximal activity of GST was at pH 6.5 and it was stable in the pH range of 5 to 10. The optimum temperature was 55°C and the Tm was 57°C. The chemical chaperone glycerol (3.3 M) was able to protect GST activity and aggregation against thermal denaturation by stabilizing the protein structure at 50 and 57°C, respectively. However, L-arginine (125 mM) did not protect GST against thermal stress. Far-ultraviolet circular dichroism (CD) spectra showed that glycerol protected the secondary structure of GST while L-arginine induced conformational changes under thermal stress. In conclusion, our studies on the GST stability suggest that glycerol works as a stabilizer and L-arginine acts as a destabilizer.
Error Analysis of Coefficient-Based Regularized Algorithm for Density-Level Detection

Abstract In this letter, we consider a density-level detection (DLD) problem by a coefficient-based classification framework with -regularizer and data-dependent hypothesis spaces. Although the data-dependent characteristic of the algorithm provides flexibility and adaptivity for DLD, it leads to difficulty in generalization error analysis. To overcome this difficulty, an error decomposition is introduced from an established classification framework. On the basis of this decomposition, the estimate of the learning rate is obtained by using Rademacher average and stepping-stone techniques. In particular, the estimate is independent of the capacity assumption used in the previous literature.
Insulin Gene Region-Encoded Susceptibility to IDDM Maps Upstream of the Insulin Gene

The gene region on chromosome 11p15.5 known to be involved in insulin-dependent diabetes mellitus (IDDM) susceptibility was recently mapped to a 4.1-kilobase region including the insulin gene. The region contains 10 candidate polymorphisms that are in strong linkage disequilibrium. By genotyping 7 of these 10 polymorphisms and the tyrosine hydroxylase microsatellite in Finnish Caucasoid IDDM patients and control subjects, we demonstrate that many of the polymorphisms found to be associated with IDDM in other Caucasoid populations do not show any association in this Finnish population. Of the polymorphisms typed, only those at –23 Hph I and the variable number of tandem repeats (VNTR) sites confer significant relative risk. Furthermore, we have demonstrated that the –23 Hph I polymorphism cannot explain the association. Comparison of the genotypic patterns observed here and previously suggests that the VNTR is the most likely candidate for IDDM2. The VNTR is located adjacent to defined regulatory DNA sequences affecting insulin gene expression, which suggests a possible effect on expression of insulin or one of the neighboring genes, tyrosine hydroxylase or insulin-like growth factor 2.
Children and young people's preference of thematic design and colour for their hospital environment

In this innovative project, the views of children and young people were explored regarding their preference of thematic design and colour for their hospital environment in a new children's unit. The novelty of the approach was that it was driven by the preferred choices of children and young people through the use of `child-friendly' interviews and questionnaires. Informing the study was the development of a group of children and young people who underwent research training, and with support, developed all data collection tools and helped to verify data analysis. A two-phased sequential study was undertaken. During phase 1, 40 interviews were performed with children and young people, including 10 with additional learning needs and physical disabilities while 140 questionnaires were analysed for phase 2 of the study. Notable issues emerged about preferred thematic designs of walls, doors and floors, while new findings were revealed regarding colour preferences for wards, entrances and outpatient areas.
Providing developmentally appropriate psychosocial care to adolescent and young adult cancer survivors

To deliver developmentally appropriate psychosocial care, the key developmental tasks facing adolescents and young adults (AYA) need to be taken into consideration. These include establishing autonomy from parents; a personal set of values and identity; strong peer relationships, including intimate and sexual relationships; and obtaining adequate preparation to join the workforce. To minimize the amount of disruption caused by the cancer experience and to maximize the health‐related quality of life of AYA patients, young individuals with cancer need opportunities to participate as much as possible in typical AYA activities and to master the developmental tasks of this life stage. Promoting a sense of normalcy is essential. To achieve this, the health care environment must be flexible and recognize the important role of peers. Informational and practical supports also are necessary for AYA to stay on track developmentally in the context of coping with cancer. Critical elements of effective AYA psychosocial services should include access to AYA‐specific information and support resources, fertility and sexuality counseling, programs to maximize academic and vocational functioning, and financial support. Cancer 2011;117(10 suppl):2329–34. © 2011 American Cancer Society.
A SINR maximizing 2D RAKE receiver for multi-sensor WCDMA mobile terminals

The 2D RAKE receiver is a spatio-temporal matched filter (MF), matched to the operations of spreading, pulse-shape filtering and spatio-temporal channel filtering. An SINR maximizing linear receiver may perform much better. In the downlink, in which the channel is the same for all intracell signals, and with orthogonal codes and cell-dependent scrambling, good SINR performance can be attained with a RAKE-like receiver. In particular, we replace the pulse-shape MF with another FIR filter, and the sparse propagation channel MF by another sparse filter. The FIR filter can be spatio-temporal or just temporal. In the latter case, it can be identical or different for the different antennas. The sparse filter is spatio-temporal. We compare different choices for the design of the FIR filter and the sparse filter.
Computer‐Assisted Quantitative Sensorimotor Testing in Patients with Carpal and Cubital Tunnel Syndromes

This study reports the use of computer-assisted sensorimotor testing in 75 patients with chronic peripheral nerve compression. Pinch and grip strength, and the cutaneous pressure threshold were measured. The reliability of this equipment for repeated measurements was excellent (r = 0.95). The pressure threshold at which a one-point static stimulus could be distinguished from a two-point static stimulus was found to be the first variable to become abnormal with computer-assisted sensorimotor testing. Criteria are suggested for screening with this equipment to detect carpal and/or cubital tunnel syndrome.
A Low-Cost Wideband 77-GHz Planar Butler Matrix in SIW Technology

This communication presents a low-cost 77-GHz switched-beam slot antenna array driven by a Butler matrix. In the proposed configuration, four broadband couplers are combined and crossovers are effectively avoided. In this case, the overall circuit and beamforming network losses are considerably reduced. A 4 × 4 planar SIW Butler matrix is designed and demonstrated for integrated beamforming network applications, which exhibits about 7 degrees phase error and ±0.75 dB coupling imbalance over 11% bandwidth. This experimentally prototyped matrix is integrated with a four-array slot antenna on the same substrate. An alternating displacement of the slots in subsequent radiating waveguides is proposed to save the arrangement of the input ports and to achieve broadband performances. Measured radiation patterns are in good agreement with simulated counterparts over the proposed 77-GHz frequency range.