Miracle-dz/UniFAR-FTUs huggingface dataset:
Profiling Human Androgen Receptor Mutations Reveals Treatment Effects in a Mouse Model of Prostate Cancer

Gain-of-function mutations in the androgen receptor (AR) are found in prostate cancer and are implicated in the failure of hormone therapy. Most studies have emphasized the ligand-binding domain (LBD) where mutations can create promiscuous receptors, but mutations in the NH2-terminal transactivation domain have also been found. To assess AR alteration as a mechanism of treatment resistance, a mouse model (h/mAR-TRAMP) was used in which the murine AR coding region is replaced by human sequence and prostate cancer initiated by a transgenic oncogene. Mice received either no treatment, androgen depletion by castration, or treatment with antiandrogens, and 20 AR transcripts were sequenced per end-stage tumor. All tumors expressed several mutant alleles, although most mutations were low frequency. Some mutations that occurred multiple times within the population were differentially located dependent on treatment. Mutations in castrated or antiandrogen-treated mice were widely dispersed but with a prominent cluster in the LBD (amino acids 736-771), whereas changes in intact mice centered near the NH2-terminal polymorphic glutamine tract. Functional characterization of selected LBD mutant alleles showed diverse effects on AR activity, with about half of the mutations reducing transactivation in vitro. One receptor, AR-R753Q, behaved in a cell- and promoter-dependent manner, although as a germ-line mutation it causes androgen insensitivity syndrome. This suggests that alleles that are loss of function during development may still activate a subset of AR targets to become gain of function in tumorigenesis. Mutant ARs may thus use multiple mechanisms to evade cancer treatment. (Mol Cancer Res 2008;6(11):1691–701)
Targeted Suppression of Chaperone-Mediated Autophagy by miR-320a Promotes α-Synuclein Aggregation

Chaperone-mediated autophagy (CMA) is involved in wild-type α-synuclein degradation in Parkinson’s disease (PD), and LAMP2A and Hsc 70 have recently been indicated to be deregulated by microRNAs. To recognize the regularory role of miR-320a in CMA and the possible role in α-synuclein degradation, in the present study, we examined the targeting and regulating role of miR-320 in Hsc 70 expression. We first constructed an α-synuclein-overexpressed human neuroblastoma cell line, SH-SY5Y-Syn(+), stably over-expressing wild-type α-synuclein and sensitive to an autophagy inhibitor, which exerted no effect on the expression of LAMP2A and Hsc 70. Then we evaluated the influence on the CMA by miR-320a in the SH-SY5Y-Syn(+) cells. It was shown that miR-320a mimics transfection of specifically targeted Hsc 70 and reduced its expression at both mRNA and protein levels, however, the other key CMA molecule, LAMP2A was not regulated by miR-320a. Further, the reduced Hsc 70 attenuated the α-synuclein degradation in the SH-SY5Y-Syn(+) cells, and induced a significantly high level of α-synuclein accumulation. In conclusion, we demonstrate that miR-320a specifically targeted the 3' UTR of Hsc 70, decreased Hsc 70 expression at both protein and mRNA levels in α-synuclein-over-expressed SH-SY5Y cells, and resulted in significant α-synuclein intracellular accumulation. These results imply that miR-320a might be implicated in the α-synuclein aggravation in PD.
Are We All on the Spectrum? Assessing Autistic Traits in the HEXACO Personality Framework

Are We All on the Spectrum? Assessing Autistic Traits in the HEXACO Personality Framework Emily R. Stiner Autistic traits are characterized by difficulties with socialization, preference for order, and rigid and repetitive behaviour patterns. This study evaluated the psychometric properties of two measures of autistic traits, the Autism-Spectrum Quotient (AQ) and the Sub-threshold Autistic Trait Questionnaire (SATQ), and their associations with the HEXACO personality framework. The relationships between self-objectification, Need for Cognition (NFC), and autistic traits were also examined. In a student sample (N = 294), autistic traits were found to be negatively related to eXtraversion, but unrelated to self-objectification and NFC. However, individual subscales of the SATQ and AQ revealed different personality profiles, suggesting a non-unitary composition of the autistic trait measures. The AQ’s subscales failed to be represented in its factor structure. Intercorrelations between SATQ and AQ subscales showed that some subscales were uncorrelated with others. These concerns challenge whether autistic traits should be considered as a downwards extrapolation of Autism Spectrum Disorder.
Contour-based Trilateration for Indoor Fingerprinting Localization

Trilateration has been widely and successfully employed to locate outdoor mobile devices due to its accuracy. However, it cannot be directly applied for indoor localization due to issues such as non-line-of-sight measurement and multipath fading. Though fingerprinting overcomes these issues, its accuracy is often hampered by signal noise and the choice of similarity metric between signal vectors. We propose INTRI, a novel, simple and effective indoor localization technique combining the strengths of trilateration and fingerprinting. For a signal level received from an access point (AP) by the target, INTRI first forms a contour consisting of all the reference points (RPs) of the same signal level for that AP, taking into account the signal noise. The target is hence at the juncture of all the contours. With an optimization formulation following the spirit of trilateration, it then finds the target location by minimizing the distance between the position and all the contours. INTRI further uses an online algorithm based on signal correlation to efficiently calibrate heterogeneous mobile devices to achieve higher accuracy. We have implemented INTRI, and our extensive simulation and experiments in an international airport, a shopping mall and our university campus show that it outperforms recent schemes with much lower location error (often by more than 20%).
Predicate-based Cache Management for Continuous Partial Match Queries in Mobile Databases

The continuous partial match query is a partial match query whose result continues to exist consistently in the client's memory. Conventional cache management methods for mobile clients are record ID-based. However, since the partial match query uses content-based retrieval, the conventional record ID-based approach cannot properly manage cache consistency. Therefore, in the paper, we propose a predicate-based cache management scheme for continuous partial match queries in mobile database systems. In the proposed cache management scheme, we represent the cache state of a mobile client as a predicate, and construct a cache invalidation report (CIR), which the server broadcasts to clients for cache management, with predicates. For reducing the amount of information that is needed in cache management, we also propose a set of methods for CIR construction (in the server) and identi cation of invalidated data (in the client). Through experiments, we show the predicate-based approach is an e ective cache management method in mobile database systems.
Design of LDPC code ensembles with fast convergence properties

The design of low-density parity-check (LDPC) code ensembles optimized for a finite number of decoder iterations is investigated. Our approach employs EXIT chart analysis and differential evolution to design such ensembles for the binary erasure channel and additive white Gaussian noise channel. The error rates of codes optimized for various numbers of decoder iterations are compared and it is seen that in the cases considered, the best performance for a given number of decoder iterations is achieved by codes which are optimized for this particular number. The design of generalized LDPC (GLDPC) codes is also considered, showing that these structures can offer better performance than LDPC codes for low-iteration-number designs. Finally, it is illustrated that LDPC codes which are optimized for a small number of iterations exhibit significant deviations in terms of degree distribution and weight enumerators with respect to LDPC codes returned by more conventional design tools.
Spectral Self-Interference Fluorescence Microscopy to Study Conformation of Biomolecules with Nanometer Accuracy

Despite the completion of the human genome sequencing, the revolution of personalized medicine still seems years away [1,2]. Although the Human Genome Project provides researchers with enormous amounts of genetic information, the genome is far more complex than the sequences it contains. Part of the reason is that DNA functions through critical interactions with proteins, such as genome packaging, epigenetic modifications, transcription, DNA replication, and DNA repair [3–7]. Since the idealized B-form DNA proposed more than 50 years ago by Watson and Crick, researchers have found that the conformation of DNA is naturally distorted and, depending on the particular sequence, DNA can be curved, tightly bent, or kinked [8–10]. These intrinsic variant conformations of DNA are recognized, stabilized, or even enhanced upon the formation of DNA–protein complexes CONTENTS
The effect of rule changes in World Series Netball: a simulation study

World Series Netball is played under different rules than standard netball. The current investigation used a simulation package to analyse the effect of two of these rule changes in isolation and when applied together. Four versions of the simulator were applied to determine match outcomes under the following conditions: • 60 minute match and alternating centre passes • 24 minute match and alternating centre passes • 60 minute match and centre passes taken by the conceding team • 24 minute match and centre passes taken by the conceding team. The superior team within a match was the team expected to convert more of its possessions into goals. The simulators showed that the superior team won 83.8% of standard netball matches. Reducing the match time to 24 minutes had a greater impact than giving the centre pass to the team that conceded the previous goal (75.5% v 83.3% of matches won by the superior team). When both rule changes were applied, the superior team won 74.6% of matches with a noticeable increase in the number of draws to 6.4% compared to 2.2% in standard netball. This study shows that simulation has a role in the development of rules in sport.
Vesicle Priming and Recruitment by ubMunc13-2 Are Differentially Regulated by Calcium and Calmodulin

Ca2+ regulates multiple processes in nerve terminals, including synaptic vesicle recruitment, priming, and fusion. Munc13s, the mammalian homologs of Caenorhabditis elegans Unc13, are essential vesicle-priming proteins and contain multiple regulatory domains that bind second messengers such as diacylglycerol and Ca2+/calmodulin (Ca2+/CaM). Binding of Ca2+/CaM is necessary for the regulatory effect that allows Munc13-1 and ubMunc13-2 to promote short-term synaptic plasticity. However, the relative contributions of Ca2+ and Ca2+/CaM to vesicle priming and recruitment by Munc13 are not known. Here, we investigated the effect of Ca2+/CaM binding on ubMunc13-2 activity in chromaffin cells via membrane-capacitance measurements and a detailed simulation of the exocytotic machinery. Stimulating secretion under various basal Ca2+ concentrations from cells overexpressing either ubMunc13-2 or a ubMunc13-2 mutant deficient in CaM binding enabled a distinction between the effects of Ca2+ and Ca2+/CaM. We show that vesicle priming by ubMunc13-2 is Ca2+ dependent but independent of CaM binding to ubMunc13-2. However, Ca2+/CaM binding to ubMunc13-2 specifically promotes vesicle recruitment during ongoing stimulation. Based on the experimental data and our simulation, we propose that ubMunc13-2 is activated by two Ca2+-dependent processes: a slow activation mode operating at low Ca2+ concentrations, in which ubMunc13-2 acts as a priming switch, and a fast mode at high Ca2+ concentrations, in which ubMunc13-2 is activated in a Ca2+/CaM-dependent manner and accelerates vesicle recruitment and maturation during stimulation. These different Ca2+ activation steps determine the kinetic properties of exocytosis and vesicle recruitment and can thus alter plasticity and efficacy of transmitter release.
A Modern Cyclic Approach to Solve a Classification Problem in Cloud Environment

As the trend changes, the data can be transit through small hand hold devices, though the small hand hold devices are being used by the users and providing simple portability, but these devices are isolate unless they connected via network. The Clouds provide more services than the hand hold devices. Through this paper, we propose A Modern Cyclic Approach to solve a Classification Problem in Cloud Environment (MCACPCE), in the area of Data Mining. Because the data warehouses are utilizing the virtualized IaaS cloud. That is, the data warehouses are accommodating in the clouds and utilizing services of cloud. We are more concentrated on classification problem in the area of data mining, for the virtual data warehouses. In the cloud hosted data warehouses the current test data set will become as training data set after some period of time. Hence we introduced the post-mortem technique on the classification model to know the facts, how good the model is induced to classify the data set from the training data set. To induce a good and an efficient classification model, the model must undergo the post-mortem operation to know the reality of the classification. The test data set must undergo the pre-processing operation to make the data set pure and clean. This process must done cyclically.
Cognitive Radio for Smart Grid Communications

One of the key foundations of smart grid (SG) is a reliable communications infrastructure which is a sophisticated, multi-layer network carrying different classes of data. SG communications needs to be designed to accommodate the current energy management requirements as well as the potential demand of future applications. In this paper, we propose the application of cognitive radio (CR) based on the IEEE 802.22 standard in the SG wide area networks (WANs). We discuss the benefits of the proposed scheme including opportunistic access of TV bands, extended coverage, ease of upgradability, self-healing and fault-tolerant design. The proposed scheme can work as a secondary radio particularly in urban areas and as a backup in disaster management. In rural areas, however, a stand-alone radio based on IEEE 802.22 can effectively provide broadband access because of the wide area coverage due to the good propagation characteristics of TV bands.
Smart Diary : the Narrative of Your Daily Life

In this paper, we present Smart Diary, a novel application integrating mobile sensing, activity inference, and natural language processing to facilitate novel knowledge representations of sensor data. Smart Diary records and analyzes a person’s daily life and transcribes those activities into human-readable outputs referred to a diary. Smart Diary makes four key contributions. First, to our best knowledge, Smart Diary is the first attempt to represent human-centric daily life through a narrative process. Second, we propose a lightweight and practical working flow in a bottom-up manner, through which we make inferences and generate diaries. Third, we present two novel models in solving context analysis issues, namely, the sustainable mining model and the narrative structured sentence model. Finally, we develop an accurate and efficient personalization/filtering algorithm in this paper so that the diary is both concise and informative.
An adaptable security manager for real-time transactions

The rising demand for real-time services over networks, such as Web-based information services, requires new approaches for balancing competing demands on limited resources. The BeeHive database system proposes a novel solution to this need by the use of adaptive real time, fault tolerance, quality of service and security services based on rules embedded in individual objects. These rules prescribe tradeoffs of alternate levels of service (and cost) when resource contention becomes a problem. The approach momentarily trades off the level of security to achieve the required real-time performance. In many situations, this is an acceptable, and even preferred, solution. We have developed an adaptable security manager to provide alternate levels of communications security to multiple users and to dynamically adapt to real-time performance conditions. In this paper, we present the design and evaluation of the proposed security manager that utilizes the notion of adaptable security services.
The effect of lignin content and lignin modification on Norway spruce wood properties and decay resistance

This thesis describes studies with three different Norway spruce cutting clones in three growing environments differing in soil and climatic conditions within the boreal zone. The main aim was to follow variation in the radial growth rate, wood properties and lignin content and to modify wood lignin with a natural monolignol, coniferyl alcohol, by making use of inherent wood peroxidases. In addition, the incorporation of chlorinated anilines into lignin was studied with synthetic model compounds and synthetic lignin preparations to show whether unnatural compounds originating from pesticides could be bound in the lignin polymer. Annual ring width, latewood proportion and wood properties such as weight density, modulus of rupture (MOR) and modulus of elasticity (MOE) were determined. Fourier transform infrared (FTIR) spectroscopy was applied to the quantitative estimation of lignin in heartwood, sapwood and earlywood samples by using a principal component regression (PCR) technique. Wood blocks were treated with coniferyl alcohol by using a vacuum impregnation method. The effect of impregnation was assessed by FTIR and by a fungal decay test. Trees from a fertile site showed the highest growth rate and sapwood lignin content and the lowest latewood proportion, weight density and MOR. Trees from a medium fertile site had the lowest growth rate and the highest latewood proportion, weight density, MOE and MOR. The most rapidly growing clone showed the lowest latewood proportion, weight density, MOE and MOR. The slowest growing clone had the lowest sapwood lignin content and the highest latewood proportion, weight density, MOE and MOR. Fairly large variation was found between the individual trees and between the growing seasons but much less variation between the sites and between the clones. The coniferyl alcohol impregnation increased the content of different lignin-type phenolic compounds in the wood as well as wood decay resistance against a white-rot fungus, Coriolus versicolor. Also the incorporation of chlorinated anilines into lignin was shown to be possible and the intermediate quinone methide was seen to be trapped by 3,4-dichloroaniline during the dehydrogenative polymerization of coniferyl alcohol, which resulted in a dimer of coniferyl alcohol with a β-O-4 structure and with dichloroaniline bound by a benzylamine bond. In conclusion, in this thesis it was shown that the different Norway spruce cutting clones maintained clone-dependent wood properties in the different growing sites although variation between tree individuals was high and climatic factors affected growth. It was also shown that the natural monolignol, coniferyl alcohol, and chlorinated anilines could be incorporated into the lignin polymer in vivo and in vitro, respectively. The coniferyl alcohol impregnation experiment resulted in the improved resistance of the wood blocks to fungal decay, while chlorinated anilines were covalently bound into the lignin polymer and they could not be released by mild acid hydrolysis.
Direct evaluation of hypersingular Galerkin surface integrals II

Direct boundary limit algorithms for evaluating hypersingular Galerkin surface integrals have been successful in identifying and removing the divergent terms, leaving finite integrals to be evaluated. This paper is concerned with the numerical computation of these multi-dimensional integrals. The integrands contain a weakly singular logarithmic term that is difficult to evaluate directly using standard numerical techniques. Herein it is shown that analytic integration of these weakly singular terms can be accomlished by suitably re-ordering the parameter integrals. In addition to improved accuracy, this process also reduces the dimension of the numerical quadrature, and hence improves efficiency.
Non-Visual Menu Navigation: the Effect of an Audio-Tactile Display

We present a preliminary study examining non-visual menu navigation in terms of task completion times and cognitive workload. We asked 12 participants to locate items on menus presented using visual, audio-only and audio-tactile displays on a touch screen mobile device and found that users were significantly slower in locating an item on a menu when using an audio-tactile menu display. This difference in performance was not reflected in the users' subjective workload assessments. We discuss the implications of these findings in terms of cross-modal display and the design of menu navigation gestures on touch screen devices.
Implications of the World Trade Center Health Program (WTCHP) for the Public Health Response to the Great East Japan Earthquake

The attacks on the World Trade Center (WTC) on September 11, 2001 resulted in a serious burden of physical and mental illness for the 50,000 rescue workers that responded to 9/11 as well as the 400,000 residents and workers in the surrounding areas of New York City. The Zadroga Act of 2010 established the WTC Health Program (WTCHP) to provide monitoring and treatment of WTC exposure-related conditions and health surveillance for the responder and survivor populations. Several reports have highlighted the applicability of insights gained from the WTCHP to the public health response to the Great East Japan Earthquake. Optimal exposure monitoring processes and attention to the welfare of vulnerable exposed sub-groups are critical aspects of the response to both incidents. The ongoing mental health care concerns of 9/11 patients accentuate the need for accessible and appropriately skilled mental health care in Fukushima. Active efforts to demonstrate transparency and to promote community involvement in the public health response will be highly important in establishing successful long-term monitoring and treatment programs for the exposed populations in Fukushima.
A Fourth Order Elliptic Low-Pass Filter with Wide Range of Programmable Bandwidth, Using Four Identical Integrators

A new method to implement elliptic filter with identical integrators and no derivatives is presented. Using identical capacitor arrays as opposed to highly spread capacitor arrays of conventional implementation makes elliptic filter design with wide range of programmable/tunable bandwidths extremely simple, time-efficient and accurate. The proposed method also reduces the die size while realizing elliptic filter more faithfully. To demonstrate the method, a fourth order elliptic filter with nine programmable bandwidths ranging from 1.1 MHz to 17.6 MHz is designed. The 1.75 mm2 filter draws 10 mA from 3.3 V supply at output swing of 4.0 V ppd in 0.35 um CMOS process to meet less than 50 nV radicHz in-band output-referred noise and SFDR of better than 80 dBc.