Miracle-dz/UniFAR-FTUs huggingface dataset:
Tartrate-Resistant Acid Phosphatase 5b in Young Patients With Sickle Cell Disease and Trait Siblings

Bone involvement is a frequent cause of acute morbidity in sickle cell disease (SCD). Tartrate-resistant acid phosphatase 5b (TRACP 5b), a bone resorption marker, is produced specifically by activated osteoclasts. We assessed bone mineral density (BMD) in 30 young patients with SCD and 17 asymptomatic patients with sickle cell trait (SCT) compared with 32 healthy controls and determined TRACP 5b levels in relation to vascular complications. Serum ferritin, alkaline phosphatase (ALP), and TRACP 5b were measured. Echocardiography was performed with assessment of BMD using dual energy X-ray absorptiometry (DXA). The BMD was decreased in patients with SCD compared with SCT and controls (P = .005), with no significant difference between the latter 2 groups. Patients with SCD had higher incidence of bone complications than SCT group and controls (P = .03). The SCD group with abnormal DXA scan had higher ferritin and ALP than normal BMD. Serum TRACP 5b was significantly higher in patients with SCD than SCT and controls (P = .003). The TRACP 5b levels were associated with severe vaso-occlusive crisis (P = .022). Patients treated with hydroxyurea and those on chelation therapy had lower TRACP 5b levels than untreated patients. The TRACP 5b level was positively correlated with lactate dehydrogenase, while there was no relation with ferritin, ALP, or BMD. We suggest that bone complications frequently occur in SCD as reflected by low BMD and high ALP and TRACP 5b. Hemolysis and iron overload may be involved in the occurrence of these complications. The lack of correlation between abnormal DXA scan and high TRACP 5b suggests that bone disease in SCD is multifactorial.
First Reported Outbreak of Diarrhea Due to Adenovirus Infection in a Hematology Unit for Adults

ABSTRACT Numerous outbreaks of adenovirus infection from different types of health care settings, except a hematology unit, have been reported. This is the first report describing an outbreak of adenovirus infection causing diarrhea among adult hematopoietic stem cell transplant recipients. Six of 21 patients from the outbreak cohort were affected with diarrhea. Electron microscopy, cell culture, and direct DNA sequencing of amplicons generated from stool and blood samples were used to investigate this outbreak. Electron microscopy and cell culture detected adenovirus in stools from symptomatic patients. DNA sequencing of amplicons generated from stool samples confirmed nosocomial transmission of infection from a single index case. The outbreak strain was also detected in plasma of four of these patients, suggesting systemic infection. The outbreak strain was identified as type 12. Standard infection control measures were effective to control this outbreak.
Altered Zn compartmentation in the root symplasm and stimulated Zn absorption into the leaf as mechanisms involved in Zn hyperaccumulation in thlaspi caerulescens

We investigated Zn compartmentation in the root, Zn transport into the xylem, and Zn absorption into leaf cells in Thlaspi caerulescens, a Zn-hyperaccumulator species, and compared them with those of a related nonaccumulator species, Thlaspi arvense. 65Zn-compartmental analysis conducted with roots of the two species indicated that a significant fraction of symplasmic Zn was stored in the root vacuole of T. arvense, and presumably became unavailable for loading into the xylem and subsequent translocation to the shoot. In T. caerulescens, however, a smaller fraction of the absorbed Zn was stored in the root vacuole and was readily transported back into the cytoplasm. We conclude that in T. caerulescens, Zn absorbed by roots is readily available for loading into the xylem. This is supported by analysis of xylem exudate collected from detopped Thlaspi species seedlings. When seedlings of the two species were grown on either low (1 &mgr;M) or high (50 &mgr;M) Zn, xylem sap of T. caerulescens contained approximately 5-fold more Zn than that of T. arvense. This increase was not correlated with a stimulated production of any particular organic or amino acid. The capacity of Thlaspi species cells to absorb 65Zn was studied in leaf sections and leaf protoplasts. At low external Zn levels (10 and 100 &mgr;M), there was no difference in leaf Zn uptake between the two Thlaspi species. However, at 1 mM Zn2+, 2.2-fold more Zn accumulated in leaf sections of T. caerulescens. These findings indicate that altered tonoplast Zn transport in root cells and stimulated Zn uptake in leaf cells play a role in the dramatic Zn hyperaccumulation expressed in T. caerulescens.
Flexible mechanisms: the diverse roles of biological springs in vertebrate movement

Summary The muscles that power vertebrate locomotion are associated with springy tissues, both within muscle and in connective tissue elements such as tendons. These springs share in common the same simple action: they stretch and store elastic strain energy when force is applied to them and recoil to release energy when force decays. Although this elastic action is simple, it serves a diverse set of functions, including metabolic energy conservation, amplification of muscle power output, attenuation of muscle power input, and rapid mechanical feedback that may aid in stability. In recent years, our understanding of the mechanisms and importance of biological springs in locomotion has advanced significantly, and it has been demonstrated that elastic mechanisms are essential for the effective function of the muscle motors that power movement. Here, we review some recent advances in our understanding of elastic mechanisms, with an emphasis on two proposed organizing principles. First, we review the evidence that the various functions of biological springs allow the locomotor system to operate beyond the bounds of intrinsic muscle properties, including metabolic and mechanical characteristics, as well as motor control processes. Second, we propose that an energy-based framework is useful for interpreting the diverse functions of series-elastic springs. In this framework, the direction and timing of the flow of energy between the body, the elastic element and the contracting muscle determine the function served by the elastic mechanism (e.g. energy conservation vs power amplification). We also review recent work demonstrating that structures such as tendons remodel more actively and behave more dynamically than previously assumed.
Practical application of synthetic biology principles

Synthetic biology can be defined as the “repurposing and redesign of biological systems for novel purposes or applications, ” and the field lies at the interface of several biological research areas. This broad definition can be taken to include a variety of investigative endeavors, and successful design of new biological paradigms requires integration of many scientific disciplines including (but not limited to) protein engineering, metabolic engineering, genomics, structural biology, chemical biology, systems biology, and bioinformatics. This review focuses on recent applications of synthetic biology principles in three areas: (i) the construction of artificial biomolecules and biomaterials; (ii) the synthesis of both fine and bulk chemicals (including biofuels); and (iii) the construction of “smart” biological systems that respond to the surrounding environment.
Study of cell viability and thermal effects under high-frequency composite pulses

Muscle contraction and the non-uniform distribution of electric field have become the key obstacles which restrict the development of the irreversible electroporation in clinical application. To solve these two problems, a novel type of pulses called high-frequency composite pulses were proposed. The killing effect and the thermal effect were investigated in this paper. The high-frequency composite pulses with different amplitudes and inner frequencies of burst were used to treat the human melanoma cells (Gll19), then the cell viability and the temperature rising under different parameters were evaluated. The results showed that, the cell viability would decrease with the increase of the electrical field amplitude (1kV/cm–2.5kV/cm), and the change of the temperature (4 °C–40 °C) was square of the amplitude. However, the inner frequency of burst had little effect on the temperature rising, and the cell viability had a slight decrease with the increase of the frequency. Therefore, the appropriate electric field strength should be selected to balance the killing effect and the thermal effect, while the inner frequency of the burst in the certain range had insignificant effect on both the cell viability and temperature rising. According to the requirement of the uniform electric field and the inhabitation of the muscle contraction, the optimal inner frequency with appropriate frequency content should be determined. This study revealed the relationship between the high-frequency composite pulse parameters and the cell viability, also the thermal effect, which was meaningful for the further investigation of the novel type of pulses.
Modeling and Real-Time Scheduling of DC Platform Supply Vessel for Fuel Efficient Operation

DC marine architecture integrated with variable speed diesel generators (DGs) has garnered the attention of the researchers primarily because of its ability to deliver fuel efficient operation. This paper aims in modeling and to autonomously perform real-time load scheduling of dc platform supply vessel (PSV) with an objective to minimize specific fuel oil consumption (SFOC) for better fuel efficiency. Focus has been on the modeling of various components and control routines, which are envisaged to be an integral part of dc PSVs. Integration with photovoltaic-based energy storage system (ESS) has been considered as an option to cater for the short time load transients. In this context, this paper proposes a real-time transient simulation scheme, which comprises of optimized generation scheduling of generators and ESS using dc optimal power flow algorithm. This framework considers real dynamics of dc PSV during various marine operations with possible contingency scenarios, such as outage of generation systems, abrupt load changes, and unavailability of ESS. The proposed modeling and control routines with real-time transient simulation scheme have been validated utilizing the real-time marine simulation platform. The results indicate that the coordinated treatment of renewable-based ESS with DGs operating with optimized speed yields better fuel savings. This has been observed in improved SFOC operating trajectory for critical marine missions. Furthermore, SFOC minimization at multiple suboptimal points with its treatment in the real-time marine system is also highlighted.
A Modified Tracking Algorithm for UWB Pilot-Assisted Receivers

The satisfactory performance of classical tracking circuits presumes that acquisition (coarse synchronization) has provided a good estimate of the correct symbol or frame timing (typically within a fraction of the chip duration). This might not be a reasonable assumption for pilot-assisted UWB receivers in dense multipath, where initial acquisition of the LOS component is highly problematic, and coarse synchronization is more likely to detect an arbitrary multipath component. In this paper, we propose a tracking algorithm for pilot-assisted receivers based on a variant of the early-late gate approach, where the circuit's correlation template is matched to the received pulse shape rather than the transmit pulse shape. The algorithm is mathematically analyzed then tested by simulation, and it is shown that it can potentially correct timing errors much larger than the transmit pulse duration
Mentoring New Social Work Faculty

Abstract The John A. Hartford Foundation, in collaboration with the Gerontological Society of America, has developed new models to create geriatric faculty capacity within social work. The Faculty Scholars Program is building faculty leadership in academic geriatric social work through a strategic approach that includes long-distance national and institution-based mentoring. While mentoring models have proven to be effective means of career development, this is relatively new in academic social work and little is known about the impact of long-distance mentoring in association with sponsorship from a mentor at the scholar's home institution. This paper describes the mentoring model and its conceptual underpinnings. Evaluation data from an ongoing evaluation is presented in discussion of the strengths and limitations of the mentoring model.
Monoclonal Antibody against the Plasmodium falciparum Chitinase, PfCHT1, Recognizes a Malaria Transmission-Blocking Epitope in Plasmodium gallinaceum Ookinetes Unrelated to the Chitinase PgCHT1

ABSTRACT To initiate invasion of the mosquito midgut, Plasmodium ookinetes secrete chitinases that are necessary to cross the chitin-containing peritrophic matrix en route to invading the epithelial cell surface. To investigate chitinases as potential immunological targets of blocking malaria parasite transmission to mosquitoes, a monoclonal antibody (MAb) was identified that neutralized the enzymatic activity of the sole chitinase of Plasmodium falciparum, PfCHT1, identified to date. This MAb, designated 1C3, previously shown to react with an apical structure of P. falciparum ookinetes, also reacts with a discrete apical structure of P. gallinaceum ookinetes. In membrane feeding assays, MAb 1C3 markedly inhibited P. gallinaceum oocyst development in mosquito midguts. MAb 1C3 affinity isolated an ∼210-kDa antigen which, under reducing conditions, became a 35-kDa antigen. This isolated 35-kDa protein cross-reacted with an antiserum raised against a synthetic peptide derived from the P. gallinaceum chitinase active site, PgCHT1, even though MAb 1C3 did not recognize native or recombinant PgCHT1 on Western blot. Therefore, this affinity-purified 35-kDa antigen appears similar to a previously identified protein, PgCHT2, a putative second chitinase of P. gallinaceum. Epitope mapping indicated MAb 1C3 recognized a region of PfCHT1 that diverges from a homologous amino acid sequence conserved within sequenced chitinases of P. berghei, P. yoelii, and P. gallinaceum (PgCHT1). A synthetic peptide derived from the mapped 1C3 epitope may be useful as a component of a subunit transmission-blocking vaccine.
Proper Gcn5 histone acetyltransferase expression is required for normal anteroposterior patterning of the mouse skeleton

Histone acetylation plays important roles in gene regulation. However, the functions of individual histone acetyltransferases (HATs) in specific developmental transcription programs are not well defined. To define the functions of Gcn5, a prototypical HAT, during mouse development, we have created a series of mutant Gcn5 alleles. Our previous work revealed that deletion of Gcn5 leads to embryonic death soon after gastrulation. Embryos homozygous for point mutations in the catalytic center of Gcn5 survive longer, but die soon after E16.0 and exhibit defects in cranial neural tube closure. Embryos bearing a hypomorphic Gcn5flox(neo) allele also exhibit neural closure defects and die at or soon after birth. We report here that Gcn5flox(neo)/flox(neo) and Gcn5flox(neo)/Δ embryos exhibit anterior homeotic transformations in lower thoracic and lumbar vertebrae. These defects are accompanied by a shift in the anterior expression boundary of Hoxc8 and Hoxc9. These data provide the first evidence that Gcn5 contributes to Hox gene regulation and is required for normal anteroposterior patterning of the mouse skeleton.
Dynastic Precautionary Savings

This article documents that parents accumulate savings to insure their children against income risk. I refer to this behaviour as dynastic precautionary saving. Using a sample of matched parent–child pairs from the Panel Study of Income Dynamics, I test for dynastic precautionary savings by examining the response of parental consumption to the child’s permanent income uncertainty. I exploit variation in permanent income risk across age and industry–occupation groups to confirm that, all else equal, higher uncertainty in the child’s permanent income depresses parental consumption, indicating a precautionary saving motive across generations.
Self-Governance and Punishment: An Experimental Study among Namibian Forest Users

We use a framed field experiment to assess resource harvesting behavior and its interaction with prosocial and antisocial punishment in the Kavango woodland savannah of Namibia. We implement two treatments, one with external, centralized punishment and one with internal, decentralized punishment. Our findings suggest that institution type matters, as internal punishment is a more effective regime to discipline high harvesters compared with external punishment. We find that antisocial punishment (i.e., the sanctioning of people who cooperate by free riders) happens frequently, partly as revenge and especially in ethnically heterogeneous groups, but ultimately does not prevent cooperative self-governance.
Single feature polymorphism detection using recombinant inbred line microarray expression data

MOTIVATION The Affymetrix GeneChip microarray is currently providing a high-density and economical platform for discovery of genetic polymorphisms. Microarray data for single feature polymorphism (SFP) detection in recombinant inbred lines (RILs) can capitalize on the high level of replication available for each locus in the RIL population. It was suggested that the binding affinities from all of the RILs would form a multimodal distribution for a SFP. This motivated us to estimate the binding affinities from the robust multi-array analysis (RMA) method and formulate the SFP detection problem as a hypothesis testing problem, i.e. testing whether the underlying distribution of the estimated binding affinity (EBA) values of a probe is unimodal or multimodal. RESULTS We developed a bootstrap-based hypothesis testing procedure using the 'dip' statistic. Our simulation studies show that the proposed procedure can reach satisfactory detection power with false discovery rate controlled at a desired level and is robust to the unimodal distribution assumption, which facilitates wide application of the proposed procedure. Our analysis of the real data identified more than four times the SFPs compared to the previous studies, covering 96% of their findings. The constructed genetic map using the SFP markers predicted from our procedure shows over 99% concordance of the genetic orders of these markers with their known physical locations on the genome sequence. AVAILABILITY The R package 'dipSFP' can be downloaded from http://sites.google. com/a/bioinformatics.ucr. edu/xinping-cui/home/software.
Freeze-fracture planes of methanogen membranes correlate with the content of tetraether lipids

Methanospirillum hungatei GP1 contained 50% of its ether core lipids (polar lipids less head groups) as tetraether lipids, and its plasma membrane failed to fracture along its hydrophobic domain during freeze-etching. The membrane of Methanosaeta ("Methanothrix") concilii did not contain tetraether lipids and easily fractured to reveal typical intramembranous particles. Methanococcus jannaschii grown at 50 degrees C contained 20% tetraether core lipids, which increased to 45% when cells were grown at 70 degrees C. The frequency of membrane fracture was reduced as the membrane-spanning tetraether lipids approached 45%. As the tetraether lipid content increased, and while fracture was still possible, the particle density in the membrane increased; these added particles could be tetraether lipid complexes torn from the opposing membrane face. The diether membrane (no tetraether lipid) of Methanococcus voltae easily fractured, and the intramembranous particle density was low. Protein-free liposomes containing tetraether core lipids (ca. 45%) also did not fracture, whereas those made up exclusively of diether lipids did split, indicating that tetraether lipids add considerable vertical stability to the membrane. At tetraether lipid concentrations below 45%, liposome bilayers fractured to reveal small intramembranous particles which we interpret to be tetraether lipid complexes.
A wavelet-based strong clutter removal technique for UWB life detection

Due to the advantages of high range resolution, good penetration through nonmetallic materials, the ground penetrating radar (GPR) with ultra-wideband (UWB) has played an important role in detection and localization of trapped victims in ruins after earthquakes and other catastrophes as well as acts of terrorism. However, the weak vital signal of the survivor can be easily contaminated by the strong clutters reflected from the ground or obstacles surround the victim, so it becomes difficult to extract human signal from the echo. In this paper, a new technique based on the discrete wavelet transform (DWT) is proposed to remove the strong clutters. And experiments are conducted in a simulated ruins with thick obstacles surround the human subject. The results indicate that by using this method, the signal-to-noise ratio (SNR) of detection data is significantly improved, thus the weak vital signal of survivor buried under ruins can be accurately detected.
Fast Distance detection by using Lock-in-amplifier in Optical Scanning Holography

We propose simple distance extraction algorithm for hologram reconstruction in optical scanning holography. By measuring the time-of-flight of the heterodyne beam in optical scanning holography, we can extract the depth information from the object apart by an arbitrary distance. For the timeof-flight, we has constructed the scanning holography and then, we extracted the phase information by using lock-in-amplifier without an additional changes in basic scanning holography setup. Finally, we have reconstructed the reflection-type complex hologram about 10.4mm x 10.4mm object apart from 426mm by this method.
The Effect of the GFC on the Distribution of Wealth in Australia

The recent Global Financial Crisis has renewed interest in levels of social inequality on both a global and a national level. Social inequality is generally conceptualised in terms of economic inequality related to income, ignoring the effects that wealth inequality has on well-being. This paper examines levels of wealth inequality in households with at least one person aged 50 years or more using the 2006 and 2010 waves of the Household Income and Labour Dynamics in Australia data. The results show that although there was a general decline in wealth holdings, wealth inequality at the household level increased dramatically between 2006 and 2010.