Miracle-dz/UniFAR-FTUs huggingface dataset:
CT-guided biopsy with cutting-edge needle for the diagnosis of malignant lymphoma: experience of 267 biopsies.

AIM We performed a retrospective study of 267 core needle aspiration biopsies in order to estimate the accuracy of CT-guided aspiration core needle biopsies for the diagnosis and subsequent treatment of malignant lymphoma. MATERIALS AND METHODS Between 1989 and 1999, 267 CT-guided core needle biopsies were performed in 241 patients with either primary or recurrent malignant lymphoma. Patients age ranged from 4--88 years. One hundred and sixty-six (62.2%) nodal and 101 (37.8%) extranodal aspiration biopsies were performed using either 18G or 20G Turner needles. Statistical method used was Chi-square analysis. RESULTS An accurate histological diagnosis was made in 199 (82.5%) patients, the remaining 42 (17.4%) patients had non-diagnostic CT biopsies. Thirty-seven of them were diagnosed by a surgical biopsy, four by bone marrow biopsy and in one patient by paracentesis. One hundred and seventy-nine patients had non-Hodgkin's lymphoma (NHL) and 62 had Hodgkin's disease (HD); 23 (9.54%) patients underwent repeated CT biopsy which was diagnostic in 17 (73.9%) and non-diagnostic in six (26%). CONCLUSION CT-guided aspiration core biopsies were sufficient to establish a diagnosis in lymphoproliferative disorders in 82.5% of cases. In the light of this experience we suggest that imaging-guided core needle biopsy be used as the first step in the work up of many patients with lymphoma.
Adaptive Redesign of Nonlinear Observers

To estimate unmeasured states in dynamical systems with parametric uncertainties one can use adaptive observers. In this technical note a new method for adaptive redesign of reduced order observers for nonlinear systems is presented. The redesign makes the observer robust as it guarantees that the state estimation error converges to zero in the presence of parametric uncertainties. To enable parameter adaption, a new type of update law is derived using Lyapunov analysis and a nonlinear coordinate transformation. The uniqueness of the approach is that it allows terms containing both uncertainty and nonlinearity in the unmeasured states to appear in the dynamics of the unmeasured states, and still achieves convergence of the state estimate without requiring persistent excitation. Two examples are presented that demonstrate these properties.
Analysis of area data rate with shadowing effects in Li-Fi and RF hybrid network

Light Fidelity (LiFi) uses light emitting diodes (LEDs) for high speed wireless communications. Since employing a different range of the electromagnetic spectrum from radio frequency (RF) communications, LiFi can significantly alleviate the traffic bottlenecks in high density RF scenarios, typically present in an indoor environment. Hence, a combination of LiFi and RF networks becomes a promising candidate for future indoor wireless communications. In a practical indoor scenario, the optical interference from neighbouring LiFi access points (APs) and the blockages of line-of-sight (LoS) optical channels induced by people and objects are the main factors that cause significant optical channel variations. In this study, the effect of these two factors on the system throughput of a hybrid LiFi/RF network is investigated. In order to offer a fair comparison, area data rate, which is defined as the system throughput in a unit area, is used for performance evaluation. The simulation shows that there is an optimal distance between two neighbouring LiFi APs to achieve the highest area data rate. In addition, the area data rate increases with the density of blockages when the blockage density is below a certain threshold.
Sustainable development of production on the base of statistical risk analysis

In the work is formalized the problem of sustainable development of production, i.e. the optimum choice of parameter values of technological process with the purpose of minimization of the risk of obtaining the production of unplanned quality and of making incorrect decision about the quality of production and maximization of production profit at the guaranteed social and economic effects. Different statements of the problem depending on the set ultimate goal are considered. The general method of solution of the put task using the Bayesian approach of many hypotheses testing is offered.
The effect soaking pea seeds with or without seedcoats has on seedling growth.

Pea seeds (Pisum sativum L. ;Alaska') with intact seedcoats (WC) and with seedcoats removed (WOC) were soaked in distilled water for 24 hours at 20 degrees . The water, containing the pea diffusate, was decanted after the second, fourth, sixth, eighth, twelfth, and twenty-fourth hour and analyzed for total nitrogen, alpha-amino nitrogen, carbohydrate, and total solute dry weight. The seeds were germinated at 20 degrees in a 16 hour photoperiod of 300 foot candles. Stem lengths and dry weights of roots, shoots and cotyledons were determined after 4, 11, and 18 days of growth. WOC seeds imbibed more water than WC seeds during the 24 hour imbibition period. Diffusates from WOC seeds always contained more solute than diffusates from WC seeds. Maltose, glucose, and fructose were not detected in the early diffusates from WOC seeds but were found in WC seed diffusates at all times. Seedlings from WC seeds had longer stems than those from WOC seeds. The dry weight of stems and roots of WC seedlings was greater than those from WOC seedlings. The dry weight of cotyledons from 18 day-old WC seedlings was less than from WOC seedlings. Water absorption by WC seeds was slower than by WOC seeds. Removal of the seedcoat allowed rapid imbibition resulting in seed injury presumably because of the loss of solutes which included monosaccharides, disaccharides, amino acids, and other nitrogen containing compounds. These results are consistent with the hypothesis that rapid imbibition disrupts membrane organization leading to reduction of seedling growth.
Bulk-Driven CMOS Amplifier With High EMI Immunity

This paper presents a bulk-driven CMOS amplifier with high immunity to electromagnetic interference (EMI). The approach is based on a bulk-driven input stage of MOSFET, thereby providing a low-voltage realization of a gate-driven stage. The bulk-driven amplifier includes the positive feedback, an input voltage drop, and dual input-stage symmetrical topology to improve the equivalent transconductance, dc linearity, and ac performance. As demonstrated by the measurements, the dc offset of the proposed amplifier is less than 70 mV, the measured PSD is about -66 dBm with 1 -Vpp EMI conveyed. The amplifier chip is implemented using a 0.35 μm standard CMOS process with a 1-V power supply.
mgr, a Novel Global Regulator in Staphylococcus aureus

ABSTRACT The virulence determinants of Staphylococcus aureus are coordinately controlled by several unlinked chromosomal loci. Here, we report the identification of CYL5614, derived from strain Becker, with a mutation that affects the expression of type 8 capsular polysaccharide (CP8), nuclease, alpha-toxin, coagulase, protease, and protein A. This novel locus, named mgr, was linked by transposon Tn917 and mapped by three-factorial transduction crosses. The region containing the mgr locus was cloned and sequenced. Deletion mutagenesis and genetic complementation showed that the locus consisted of one gene, mgrA. Interestingly, mgrA-null mutants exhibited a phenotype opposite to that of CYL5614. This was due to a T-to-C mutation upstream of mgrA that resulted in a four- to eightfold increase in mgrA transcription in strain CYL5614. Thus, these results indicate that mgrA is an activator of CP8 and nuclease but a repressor of alpha-toxin, coagulase, protease, and protein A. In addition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses showed that the mgr locus profoundly affected extracellular protein production, suggesting that the locus may regulate many other genes as well. The translated MgrA protein has a region of significant homology, which includes the helix-turn-helix DNA-binding motif, with the Escherichia coli MarR family of transcriptional regulators. Northern slot blot analyses suggested that mgr affected CP8, alpha-toxin, nuclease, and protein A at the transcriptional level.
Wideband and Multipolarization Reconfigurable Crossed Bowtie Dipole Antenna

A simple design of a wideband antenna with the capability of switching its operation among five polarization states is proposed. The primary radiator consists of two bowtie dipoles arranged orthogonally through double printed rings. The polarization-reconfigurability is realized by electronically switching three p-i-n-diode pairs. By controlling the current paths from the feeding coaxial line to each dipole arm and among these arms, the antenna can change its radiation among circular polarization (CP) with both rotating senses and linear polarization at three different angles, namely, 0°, 90°, and 45°. The fabricated prototype exhibits an impedance bandwidth (BW) of 37.1% from 2.2 to 3.2 GHz for all polarization states. The axial ratio BW for both CP states is 50.4%, covering the above frequency range. A measured realized gain of greater than 6.6 dBi and a radiation efficiency of around 80% over the overlapped BW are achieved for all states.
Density of Biogas Power Plants as An Indicator of Bioenergy Generated Transformation of Agricultural Landscapes

The increasing use of biogas, produced from energy crops like silage maize, is supposed to noticeably change the structures and patterns of agricultural landscapes in Europe. The main objective of our study is to quantify this assumed impact of intensive biogas production with the example of an agrarian landscape in Northern Germany. Therefore, we used three different datasets; Corine Land Cover (CLC), local agricultural statistics (Agrar-Struktur-Erhebung, ASE), and data on biogas power plants. Via kernel density analysis, we delineated impact zones which represent different levels of bioenergy-generated transformations of agrarian landscapes. We cross-checked the results by the analyses of the land cover and landscape pattern changes from 2000 to 2012 inside the impact zones. We found significant correlations between the installed electrical capacity (IC) and land cover changes. According to our findings, the landscape pattern of cropland—expressed via landscape metrics (mean patch size (MPS), total edge (TE), mean shape index (MSI), mean fractal dimension index (MFRACT)—increased and that of pastures decreased since the beginning of biogas production. Moreover, our study indicates that the increasing number of biogas power plants in certain areas is accompanied with a continuous reduction in crop diversity and a homogenization of land use in the same areas. We found maximum degrees of land use homogenisation in areas with highest IC. Our results show that a Kernel density map of the IC of biogas power plants might offer a suitable first indicator for monitoring and quantifying landscape change induced by biogas production.
Decreased expression of the CFTR protein in remodeled human nasal epithelium from non-cystic fibrosis patients.

BACKGROUND In normal adult pseudostratified human nasal surface epithelium, the cystic fibrosis transmembrane conductance regulator (CFTR) is localized to the apical domain of the ciliated cells, whereas in cystic fibrosis (CF), the mutated delta F 508 CFTR exhibits an abnormal cytoplasmic localization. Frequent airway injuries either in CF or non-CF patients may induce a remodeling of the surface epithelium characterized by a change in the morphological structure from normal columnar pseudostratified epithelium to either basal cell hyperplasia, mucous cell hyperplasia, or squamous metaplasia. EXPERIMENTAL DESIGN The localization of CFTR parallel to markers of cell differentiation, such as cytokeratin 14 (CK14, a marker of basal cells), cytokeratin 18 (CK 18, a marker of ciliated and mucous cells), cytokeratin 13 (CK13, a marker of squamous metaplasia cells), and desmoplakins (DP) 1 and 2 (markers of desmosomes) was analyzed by indirect immunofluorescence. RESULTS In normal pseudostratified epithelium, CFTR was detected at the apical plasma membrane of the ciliated cells, CK14 was identified in basal cells of focal areas, CK18 was localized in both ciliated and mucous cells, CK 13 was detected in all basal cells, and DP 1 and 2 were preferentially detected at the interface between columnar and basal cells. In basal cell hyperplasia, CFTR was poorly expressed in the cytoplasm of the more superficial cells, CK14 and CK13 were localized in basal cell multilayers, CK18 labeling was present in the more superficial cell layers, and DP 1 and 2 were preferentially detected at the interface between the more basal cells. In squamous metaplasia, CFTR labeling was either very low or even undetectable, CK14 was found in focal areas of the more basal cell layers, CK18 labeling was either very low or undetectable, CK13 expression was restricted to the flattened cells toward the epithelial surface, and DP 1&2 were intensively present between all the epithelial cells. CONCLUSIONS These results suggest that the localization of CFTR in human nasal surface epithelium is related to the differentiation state of this epithelium. Abnormally low expression of the CFTR protein may not only be caused by CFTR gene mutations but can also be associated with airway surface epithelium dedifferentiation and remodeling.
Cigarette Smoke Stimulates MMP‐1 Production by Human Lung Fibroblasts Through the ERK1/2 Pathway

An imbalance between proteases and anti‐proteases is believed to play an important role in the pathogenesis of emphysema. In this study, we explored the hypothesis that cigarette smoke can alter tissue structure through an effect on the release of matrix metalloproteinase‐1 (MMP‐1) and type I tissue inhibitor of metalloproteinases (TIMP‐1). Cigarette smoke extract (CSE) significantly stimulated pro‐MMP‐1 production (determined by ELISA and immunoblots) and mRNA expression (by real‐time RT‐PCR) by human fetal lung fibroblasts (HFL‐1) in a concentration‐dependent manner (2.5–10%). High concentrations of CSE (10%) could potentially activate the latent form of MMP‐1 as the high molecular weight (52 kDa) form was converted into a low molecular weight (42 kDa) form consistent with active MMP‐1. TIMP‐1 production, however, was not significantly altered by the concentrations of CSE tested. After 30 min exposure, CSE significantly induced ERK1/2 phosphorylation, which then gradually decreased from 90 minutes to 3 hours. PD98059, a specific inhibitor of ERK‐MAPK, significantly blocked the CSE effect on ERK1/2 phosphorylation. Furthermore, PD98059 significantly inhibited the CSE effect on MMP‐1 production and mRNA expression by fibroblasts. These results suggest that cigarette smoke stimulates production and likely activates MMP‐1 through activating ERK1/2 signal transduction pathway. By inducing MMP‐1, cigarette smoke may result in excess tissue destruction and contribute to the development of emphysema.
Multimodality Imaging of Carotid Stenosis

Abstract Four diagnostic modalities are used to image the following internal carotid artery: digital subtraction angiography (DSA), duplex ultrasound (DUS), computed tomography angiography (CTA), and magnetic resonance angiography (MRA). The aim of this article is to describe the potentials of these techniques and to discuss their advantages and disadvantages. Invasive DSA is still considered the gold standard and is an indivisible part of the carotid stenting procedure. DUS is an inexpensive but operator-dependent tool with limited visibility of the carotid artery course. Conversely, CTA and MRA allow assessment of the carotid artery from the aortic arch to intracranial parts. The disadvantages of CTA are radiation and iodine contrast medium administration. MRA is without radiation but contrast-enhanced MRA is more accurate than noncontrast MRA. The choice of methods depends on the clinical indications and the availability of methods in individual centers. However, the general approach to patient with suspected carotid artery stenosis is to first perform DUS and then other noninvasive methods such as CTA, MRA, or transcranial Doppler US.
Ras-GTP dimers activate the Mitogen-Activated Protein Kinase (MAPK) pathway

Significance Rat sarcoma (Ras) proteins play central roles in both normal and oncogenic signaling. Mechanisms of how Ras interacts with its effectors on the cell membrane, however, are still poorly understood, significantly hampering efforts to target this molecule in human cancer. Here we have used quantitative superresolution fluorescence microscopy in combination with carefully engineered biological systems to show that Ras dimers drive oncogenic signaling through the Raf-MAPK pathway. Our study suggests a new, dimer model of Ras-Raf signaling and the potential value of Ras dimers as a therapeutic target. Rat sarcoma (Ras) GTPases regulate cell proliferation and survival through effector pathways including Raf-MAPK, and are the most frequently mutated genes in human cancer. Although it is well established that Ras activity requires binding to both GTP and the membrane, details of how Ras operates on the cell membrane to activate its effectors remain elusive. Efforts to target mutant Ras in human cancers to therapeutic benefit have also been largely unsuccessful. Here we show that Ras-GTP forms dimers to activate MAPK. We used quantitative photoactivated localization microscopy (PALM) to analyze the nanoscale spatial organization of PAmCherry1-tagged KRas 4B (hereafter referred to KRas) on the cell membrane under various signaling conditions. We found that at endogenous expression levels KRas forms dimers, and KRasG12D, a mutant that constitutively binds GTP, activates MAPK. Overexpression of KRas leads to formation of higher order Ras nanoclusters. Conversely, at lower expression levels, KRasG12D is monomeric and activates MAPK only when artificially dimerized. Moreover, dimerization and signaling of KRas are both dependent on an intact CAAX (C, cysteine; A, aliphatic; X, any amino acid) motif that is also known to mediate membrane localization. These results reveal a new, dimerization-dependent signaling mechanism of Ras, and suggest Ras dimers as a potential therapeutic target in mutant Ras-driven tumors.
Regulation of human papillomavirus type 11 enhancer and E6 promoter by activating and repressing proteins from the E2 open reading frame: functional and biochemical studies

E2-C, a protein consisting mainly of the carboxy-terminal 45% of the human papillomavirus type 11 (HPV-11) E2 protein, was expressed from the Rous sarcoma virus long terminal repeat in mammalian cells. It competitively repressed the stimulatory action of the full-length E2 protein on the HPV-11 enhancer located in the upstream regulatory region, as assayed by the expression of a reporter gene from the simian virus 40 (SV40) early promoter in transiently transfected monkey CV-1 cells. A mutation in the initiation codon for E2-C protein eliminated repression. In the human cervical carcinoma cell line C-33A, which apparently lacks endogenous HPV DNA, the HPV-11 enhancer-SV40 promoter and the HPV-11 enhancer in its normal association with the E6 promoter had high constitutive activity. In these cells, E2 proteins had little or no stimulatory effect on the transcriptional activity of the HPV-11 enhancer-SV40 promoter. In contrast, the HPV-11 enhancer-E6 promoter was stimulated by the HPV-11 E2 protein but repressed by the bovine papillomavirus type 1 E2 protein, an effect due either to a quantitative difference in E2 expression levels or to a qualitative difference in the trans-activating abilities of the two E2 proteins. In this cell line, the HPV-11 E2-C protein suppressed both the constitutive activity and the HPV-11 E2 trans activation. The E2-C protein was also produced from an expression vector in Escherichia coli. The E2-C protein present in crude E. coli lysates or purified by DNA affinity chromatography associated in vitro with a specific sequence, ACCN6GGT, in filter-binding assays. Moreover, the protein generated DNase I footprints spanning this motif identical to those of bacterially expressed full-length E2 proteins. This DNA sequence motif is necessary and sufficient for E2 binding in vitro and enhancer trans activation in vivo (H. Hirochika, R. Hirochika, T. R. Broker, and L. T. Chow, Genes Dev. 2:54-67, 1988). Mutations in this sequence that abolished interactions with E2 also precluded binding to the E2-C protein. These data strongly suggest that the full-length E2 protein consists of two functional domains: the amino-terminal half for trans activation and the carboxy-terminal half for DNA binding. The mechanism by which E2-C represses E2-dependent enhancer activity most likely involves competition with E2 for binding to a common transcriptional regulatory site. (ABSTRACT TRUNCATED AT 400 WORDS)
A novel 2-bp indel within Krüppel-like factor 15 gene (KLF15) and its associations with chicken growth and carcass traits

Abstract 1. The Krüppel-like factor (KLF) family of zinc-finger transcription factors plays a critical role in cell differentiation, phenotypic modulation and physiologic function. KLF15 has been proposed to regulate adipogenesis and gluconeogenesis. The objective of this study was to establish the association between KLF15 gene polymorphism and chicken growth and carcass traits. 2. An F2resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken KLF15 gene. A 2-bp indel mutation (G13781_13782del/insAG) within intron 2 was detected, and a polymerase chain reaction–restriction fragment length polymorphism method was developed to genotype the F2 individuals. 3. Association analysis showed that the Single Nucleotide Polymorphisms (SNP) was significantly associated with chicken growth and carcass traits. The chickens with the insAG/insAG genotype generally had a significantly higher body weight and size than other genotypes. Gene expression for each genotype showed that birds carrying insAG/insAG had a higher expression level than the other genotypes. 4. The results suggested that this polymorphic site may serve as a useful target for marker assisted selection of chicken growth and carcass traits.
The seroprevalence of human infection with Rickettsia slovaca, in an area of northern Spain

Abstract An epidemiological survey was undertaken to explore human exposure to Rickettsia slovaca in two provinces of northern Spain. When IFAT were used to test 200 members of the general population for antibodies to rickettsiae of the spotted-fever group, six (3.3%) were found positive, presumably, since Dermacentor is one of the most common genera of human-biting tick in the study area, for antibodies to R. slovaca. Thirty-one (16.9%) of an additional 183 subjects who presented shortly after being bitten by ticks were also found seropositive. The difference in seroprevalence between the general and the tick-bitten populations was significant. Subject gender had no influence on seroprevalence in either population, although, in the tick-bitten group, age and occupation did have a significant influence on the prevalence recorded. Immunoblotting was used to confirm the presence of antibodies in the five subjects, all from the tick-bitten group, found to have acute infections. Three D. marginatus ticks obtained from three of these acute cases were found PCR-positive for R. slovaca DNA.