Miracle-dz/UniFAR-FTUs huggingface dataset:
A novel fusion gene, TRIM5-Cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection

Objective:In Old World monkeys, the tripartite motif 5α (TRIM5α) protein confers resistance to HIV-1 infection following virus entry into host cells. However, the pig-tailed macaque (Macaca nemestrina) is an exception and is susceptible to HIV-1 infection. This study dissects the molecular mechanism of the pig-tailed macaque's susceptibility to HIV-1 infection. Methods:Genomic sequencing and expression analysis of the TRIM5α gene was conducted in the pig-tailed macaque. A novel TRIM5-Cyclophilin A fusion gene isoform was identified and subsequently cloned into the pcDNA3.1(+) expression vector. This construct was transfected into HeLa-T4 or HeLa cells which were then infected with the HIV-1IIIB or HIV-GFP-VSVG pseudotyped virus, to examine the effects of the TRIM5-Cyclophilin A fusion protein on HIV-1 infection. Results:A novel TRIM5-Cyclophilin A fusion gene (mnTRIMCyp) in the pig-tailed macaque was found and its fusion pattern is different from the known fusion gene in the owl monkey (owlTRIMCyp). TRIMCyp protein expression in transfected cells was confirmed by western blotting. The tests using HIV-1IIIB and HIV-GFP-VSVG pseudotyped virus indicated that mnTRIMCyp did not inhibit HIV-1 replication at various multiplicities of infection. Conclusions:The mnTRIMCyp fusion protein does not restrict replication of HIV-1, which provides a potential molecular mechanism that might explain why the pig-tailed macaque is prone to HIV-1 infection, the only known exception in Old World monkeys.
Multi-processor Memory Scoreboard: A Multi-processor Memory Ordering and Data Consistency Checker

In this paper a verification architecture called Multi-Processor Memory Scoreboard (also referred as MPMS, thechecker, the scoreboard in this paper) developed at SamsungAustin Research & Development Center is described. This isa RTL simulation based scoreboard which performs data consistency and memory ordering checks as required by ARMv8ISA memory model and finds RTL errors very close to thefailure point. Along with the knowledge of architectural memoryordering requirements, the scoreboard employs age-order andtime-order relations of instructions of the test programs andperforms data consistency and memory ordering checks.
Utility-Aware Resource Allocation for Multi-Stream Overlay Multicast

Overlay multicast, which performs topology construction and data relaying in the application layer, has recently emerged as a promising vehicle for data distribution. In most of the existing systems, only a single stream is assumed for each overlay, and multiple streams, if needed, are distributed separately. In addition, while the overlay node performs relay functions, they generally do not filter the content to match the heterogeneous bandwidth constraints. We consider a more general model, in which a multicast session may consist of multiple data streams, such as video and audio, which are to be delivered to the same set of nodes. The services to these steams are elastic through layer dropping, shaping, or transcoding. In this paper, we focus on an important resource allocation problem in such an adaptive multi-stream multicast framework: Assume that each stream has an associated utility function, how to maximize the total utility of all the streams in a multicast session. The problem involves optimization not only in individual nodes, but also in the global overlay structure. We show that, given a total bandwidth of the streams, there is an efficient and optimal solution for the allocation problem at a single node. The problem however can be much more complicated in a hierarchical structure like tree. Yet we show that, if the utility is concave with bandwidth, i.e., the marginal improvement diminishes with bandwidth increase, then the upstream constraint can be ignored and a local optimal solution is simply the global optimal solution.
Frizzled3 Controls Axonal Polarity and Intermediate Target Entry during Striatal Pathway Development

The striatum is a large brain nucleus with an important role in the control of movement and emotions. Medium spiny neurons (MSNs) are striatal output neurons forming prominent descending axon tracts that target different brain nuclei. However, how MSN axon tracts in the forebrain develop remains poorly understood. Here, we implicate the Wnt binding receptor Frizzled3 in several uncharacterized aspects of MSN pathway formation [i.e., anterior–posterior guidance of MSN axons in the striatum and their subsequent growth into the globus pallidus (GP), an important (intermediate) target]. In Frizzled3 knock-out mice, MSN axons fail to extend along the anterior–posterior axis of the striatum, and many do not reach the GP. Wnt5a acts as an attractant for MSN axons in vitro, is expressed in a posterior high, anterior low gradient in the striatum, and Wnt5a knock-out mice phenocopy striatal anterior–posterior defects observed in Frizzled3 mutants. This suggests that Wnt5a controls anterior–posterior guidance of MSN axons through Frizzled3. Axons that reach the GP in Frizzled3 knock-out mice fail to enter this structure. Surprisingly, entry of MSN axons into the GP non–cell-autonomously requires Frizzled3, and our data suggest that GP entry may be contingent on the correct positioning of “corridor” guidepost cells for thalamocortical axons by Frizzled3. Together, these data dissect MSN pathway development and reveal (non)cell-autonomous roles for Frizzled3 in MSN axon guidance. Further, they are the first to identify a gene that provides anterior–posterior axon guidance in a large brain nucleus and link Frizzled3 to corridor cell development. SIGNIFICANCE STATEMENT Striatal axon pathways mediate complex physiological functions and are an important therapeutic target, underscoring the need to define how these connections are established. Remarkably, the molecular programs regulating striatal pathway development remain poorly characterized. Here, we determine the embryonic ontogeny of the two main striatal pathways (striatonigral and striatopallidal) and identify novel (non)cell-autonomous roles for the axon guidance receptor Frizzled3 in uncharacterized aspects of striatal pathway formation (i.e., anterior–posterior axon guidance in the striatum and axon entry into the globus pallidus). Further, our results link Frizzled3 to corridor guidepost cell development and suggest that an abnormal distribution of these cells has unexpected, widespread effects on the development of different axon tracts (i.e., striatal and thalamocortical axons).
Subjective Concepts of Chronically Ill Patients Using Distant Healing

Distant healing procedures consist of benevolent intentions, often taking the form of prayers for a patient. Despite inconclusive evidence regarding distant healing, prayers are a widespread health-related technique. We studied subjective concepts of distant healing in 17 patients suffering from chronic fatigue syndrome and multiple chemical sensitivity who were given distant healing during a randomized controlled trial. We applied reconstructive interview analysis when analyzing the results. The overall theme was the tension between mainstream medicine and the immaterial healing procedure. Several components highlighted this tension: (a) patterns of legitimizing the use of distant healing, (b) distant healing and the social setting, (c) integrating distant healing into their belief system, and (d) reconstruction of effects by means of hindsight. The interviews showed that patients felt the need to legitimize having tried distant healing. They had to bear the full ambiguity of biomedicine being in competition with distant healing, though also experiencing distant healing as giving support.
Adopting an integrated catchment-based management approach for Putrajaya Lake and Wetlands

The Federal Government of Malaysia's new administrative centre, Putrajaya, is being developed with a garden concept comprising an artificial lake and wetlands to balance the rapidly changing ecosystem and alleviate the impact of urbanisation on the former rubber and palm oil plantations. This newly transformed urban ecosystem will accommodate and enhance the city's estimated future population of 300,000 people. The urbanisation involving the development of residential, government office and commercial complexes, will inevitably have an impact on the surrounding environment. The development of Putrajaya Lake and wetlands over an area of 600 hectares will create a balanced biogenic environment and subsequently advocate the importance of urban eco-hydrological management systems within catchments. This paper reviews the adoption of a new integrated catchments management approach through the example of Putrajaya in order to realise the desired level of ecosystem service and the water quality resulting from the environment.
The role of task-related antecedents for the development of turnover intentions in temporary project teams

Abstract High turnover can cause serious problems in organizations. Yet, previous research confirmed that HRM practices are useful to influence the development of turnover intentions. Existing studies have revealed a variety of factors that drive individuals to leave permanent organizations (POs) but almost no research has considered turnover in temporary organizations (TOs). Yet, TOs, such as project teams, have become increasingly prevalent and exhibit several characteristics that are distinct from POs. Hence, the antecedents of turnover intentions in TOs may also differ from those in POs. However, empirical evidence for this proposition is still lacking. In order to address this research gap, this study examines task-related antecedents of turnover intentions in temporary organizations (TITO) using a sample of 253 employees working in project teams. The results show that inter-role conflict is the most influential antecedent of TITO. Unlike suggested by extant literature, members of TOs seem to be willing to accept down-cuts in job autonomy and meaningfulness of their work, without developing turnover intentions while working for TOs. Furthermore, the results confirmed a direct relationship between TITO and turnover intentions from the permanent organization (TIPO). Members of TOs that want to, but cannot leave their project team seem to develop TIPO.
Towards automatic analysis of dynamic radionuclide studies using principal-components factor analysis

A method is proposed for automatic analysis of dynamic radionuclide studies using the mathematical technique of principal-components factor analysis. This method is considered as a possible alternative to the conventional manual regions-of-interest method widely used. The method emphasises the importance of introducing a priori information into the analysis about the physiology of at least one of the functional structures in a study. Information is added by using suitable mathematical models to describe the underlying physiological processes. A single physiological factor is extracted representing the particular dynamic structure of interest. Two spaces 'study space, S' and 'theory space, T' are defined in the formation of the concept of intersection of spaces. A one-dimensional intersection space is computed. An example from a dynamic 99Tcm DTPA kidney study is used to demonstrate the principle inherent in the method proposed. The method requires no correction for the blood background activity, necessary when processing by the manual method. The careful isolation of the kidney by means of region of interest is not required. The method is therefore less prone to operator influence and can be automated.
Vectorial targeting of apical and basolateral plasma membrane proteins in a human adenocarcinoma epithelial cell line.

We studied the surface delivery pathways followed by newly synthesized plasma membrane proteins in intestinal cells. To this end, we developed an assay and characterized an epithelial cell line (SK-CO-15) derived from human colon adenocarcinoma. Polarized confluent monolayers (2000 omega.cm2), grown on polycarbonate filter chambers, were pulsed with radioactive methionine/cysteine and, at different times of chase, the protein fraction reaching the apical or basolateral surface was recovered by domain-selective biotinylation, immunoprecipitation, and immobilized streptavidin precipitation. Both an apical and a basolateral marker were found to be delivered vectorially to the respective surface, with a sorting efficiency of 50:1 for the basolateral marker and 14:1 for the apical marker.
Early Antidepressant Treatment and All-Cause 30-Day Mortality in Patients with Ischemic Stroke

Background: Antidepressants, in particular selective serotonin reuptake inhibitors, have been associated with antithrombotic and neuroprotective properties and their more widespread use has been suggested in stroke recovery. However, data are sparse on their effects on the clinical outcome, including mortality, associated with early antidepressant treatment after stroke. We aimed to study all-cause 30-day mortality related to early antidepressant treatment in patients with ischemic stroke. Methods: We did a population-based follow-up study identifying patients from the Danish Stroke Registry admitted in the former Aarhus County from 2003 to 2010. During this time, initiation of antidepressant treatment during admission was registered in the Danish Stoke Registry. The registry also holds clinical information including stroke type, stroke severity and quality of in-hospital stroke care. Information on vital status and covariates including comorbidities and co-medication was obtained from the following population-based medical registries: the Danish Civil Registration System, Danish Medicines Agency's Medical Register and The Danish National Patients Registry. Information was linked using the unique civil registration number assigned to all Danish residents. Multivariable logistic regression was used to compute the adjusted odds ratio (OR) of 30-day mortality in patients treated with antidepressants during admission as compared to patients not treated. In addition, we did stratified analyses on sex, age, stroke severity and propensity score-matched analyses as well as multiple imputation. Results: Among 5,070 consecutive first-ever stroke patients without prior antidepressant treatment, 955 (18.8%) started antidepressant treatment during admission with a median time from admission until treatment of 5 days (interquartile range 2-11). The proportion of patients with severe stroke was higher among treated patients as compared to that among non-treated patients. The adjusted OR of 30-day mortality was 0.28 (95% confidence interval (CI) 0.18-0.43) for patients treated during admission as compared to patients not treated during admission. Stratification by stroke severity showed signs of effect modification, stratification by sex and age did not. Included in the propensity score-matched analyses were 1,908 patients matched 1:1. The propensity score-matched adjusted OR of death within 30 days was 0.31 (95% CI 0.19-0.49). Conclusion: Although early antidepressant treatment was more often started in patients with severe stroke, treatment was associated with significantly lower mortality. This result requires replication in randomized trials; however, it indicates that early start of antidepressant treatment after stroke may be safe and a more routine use may be feasible.
Analysis and Design Guide of Active EMI Filter in a Compact Package for Reduction of Common-Mode Conducted Emissions

A common-mode (CM) active filter was designed in a compact package to suppress CM conducted emissions at a switching mode power supply (SMPS). Based on the analytical expressions considering both stability and performance, the design and optimization rules for the proposed active filter have been presented. After verifying its performance by measurements using vector network analysis, the proposed filter was installed in a 200-W SMPS board with 64 and 110 kHz switching frequencies, demonstrating its usefulness by experiments. The performance degradation due to the magnetic saturation and the AEF grounding impedance was also analyzed and investigated.
The empirics of enabling investment and innovation in renewable energy

This working paper undertakes econometric analysis to assess the impacts of climate mitigation policies and the quality of the investment environment on investment and innovation in renewable power in OECD and G20 countries. It also assesses how countries’ investment environments interact with climate mitigation policies to influence investment and patent activity in renewable power. The paper gathered and tested data across OECD and G20 countries on more than 70 explanatory variables, which were analysed using two Poisson-family regression models: one to investigate determinants of investment flows in renewable power from 2000 until 2014; and one to investigate determinants of patent counts in renewable-power technologies from 2000 until 2012. Results of the econometric analysis are consistent with the main hypothesis in this paper that beyond setting climate mitigation policies, policy makers need to strengthen the general investment environment and align it with climate mitigation policies in order to mobilise investment and innovation in renewable power across OECD and G20 countries.
Pricing reactive power: Some challenges

Distributed generation as attached to market liberalization requires pricing schemes able to deliver the appropriate incentives to market participants. In particular, users should be made responsible for their reactive power. A penalty system fails in that account. One of the major challenges in setting up an appropriate pricing of reactive power consists in identifying its attached costs. Although their peculiar structure might suggest as appropriate a separate treatment of real and reactive power, we argue for a joint approach. In fact, what matters is the total financial burden each user has to support, not the way it is actually computed. A fairness criteria is introduced, according to which no user or group of users should contribute more than its stand-alone cost. When contributions exactly cover total costs, this implies that no user is a burden to the others. We evidence that this criteria is nonsensical if applied to only part of the costs. We conclude by proposing a pricing rule that always complies with our fairness requirements. Its practical implementation is presented and applied to an illustrative example. Tariff penalties for lack of power factor compensation are analyzed for two energy suppliers, one in Europe, the other in North America. They are shown to (possibly) fail to comply with our fairness criteria.
Increased dermal elastic fibers in the tight skin mouse.

OBJECTIVE The tight skin (Tsk-1) mouse has been proposed as a model for systemic sclerosis on the basis of increased accumulation of collagen and glycosaminoglycans in the skin, and by the presence of serum autoantibodies. The genetic basis of the mutation has been identified as a genomic duplication within the fibrillin-1 (Fbn-1) gene that results in a larger than normal Fbn-1 transcript, but the mechanism that leads to dermal fibrosis is unclear Fibrillin molecules associate into a polymer that is coated with elastin molecules to form elastic fibers. To further evaluate the Tsk-1 mouse model of scleroderma, we have studied elastic fibers in the skin of these mice. METHODS Skin sections obtained from C57BL/6-TSK+ (Tsk-1) and C57BL6-pa/+ (control) mice were stained with Masson's trichrome for evaluation of collagen and Gomori's aldehyde fuchsin stain for elastic tissue. Computer assisted image analysis was performed to quantify differences in histologic sections. RESULTS Tsk-1 mice had a highly significant increase in the percentage of elastic fibers (19.6%) in the dermis compared to control mice (7.9%) [p < 0.001]. This correlates with the findings in the skin of systemic sclerosis patients where increased elastic fibers have been observed. In addition, an increased level of dermal collagen staining was also observed in the Tsk-1 dermis (82.9%) compared with the level in normal sections (73.7%) [p < 0.01]. CONCLUSION These data support the use of the Tsk-1 mouse as a model for the connective tissue abnormalities of human scleroderma.
An Illumination- and Temperature-Dependent Analytical Model for Copper Indium Gallium Diselenide (CIGS) Solar Cells

In this paper, we present a physics-based analytical model for copper indium gallium diselenide (CIGS) solar cells that describes the illumination- and temperature-dependent current-voltage (I-V) characteristics and accounts for the statistical shunt variation of each cell. The model is derived by solving the drift-diffusion transport equation so that its parameters are physical and, therefore, can be obtained from independent characterization experiments. The model is validated against CIGS I-V characteristics as a function of temperature and illumination intensity. This physics-based model can be integrated into a large-scale simulation framework to optimize the performance of solar modules, as well as predict the long-term output yields of photovoltaic farms under different environmental conditions.
Rheogenic sodium‐bicarbonate co‐transport across the retinal membrane of the frog retinal pigment epithelium.

1. Na+ and HCO3‐ transport across the retinal membrane of the frog retinal pigment epithelium was studied by means of double‐barrelled Na(+)‐ and pH‐selective microelectrodes. Transient changes in the intracellular pH and in the intracellular Na+ activity were monitored in response to abrupt changes in the Na+ concentration and in the HCO3‐ concentration on the retinal side of the epithelium, and in response to transepithelial currents. 2. Removal of Na+ from the retinal side of the epithelium caused a depolarization of the membrane potential across the retinal membrane, a decrease in the intracellular Na+ activity and a decrease in the intracellular pH. 3. An increase in the HCO3‐ concentration on the retinal side of the epithelium from 27.5 to 50 mM caused a hyperpolarization of the membrane potential across the retinal membrane, an increase in the intracellular Na+ activity and an increase in the intracellular pH. 4. Passage of a transepithelial current of 30 microA in the choroid‐to‐retina direction caused an increase in the intracellular Na+ activity and an increase in the intracellular pH. 5. The data are interpreted as evidence for rheogenic co‐transport of Na+, HCO3‐ across the retinal membrane of the frog retinal pigment epithelium. 6. The transient changes described under 2, 3 and 4 above were blocked by 0.5 mM‐4‐acetamido‐4'‐isothiocyanostilbene‐2.2'‐disulphonic acid (SITS). The Na(+)‐ HCO3‐ co‐transport was not inhibited by 1 mM‐amiloride.