Miracle-dz/UniFAR-FTUs huggingface dataset:
The Proteome of Human Brain After Ischemic Stroke

Although stroke is among the most common causes of death and chronic disability worldwide, the proteome of the ischemic human brain remains unknown. Only a few studies have investigated the ischemic brain proteome in rodent stroke models. We performed aproteomic study of the human brain after ischemic stroke usinga 2-dimensional differential gel electrophoresis-based proteomic approach. In brain samples from 6 deceased stroke patients and 3 control subjects, there was an average of 1,442 ± 231 protein spots in the gels. Changes of at least 1.5-fold in the relative expression of 132 protein spots between different cerebral areas (infarct core, peri-infarct, and contralateral tissue) were identified (p < 0.05); 39 of these were successfully identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Among the identified protein spots, we validated the results of 10 proteins by Western blot and determined the cellular localization in brain parenchyma for 3 of the identified proteins: dihydropyrimidinase-related protein 2, vesicle-fusing ATPase, and Rho dissociation inhibitor 1. These results contribute to understanding the processes that follow cerebral ischemia; moreover, some of the identified proteins may be therapeutic targets or biologic markers for determining the diagnosis and prognosis of stroke.
HIV Testing as a Standard of Care for Men Who Have Sex With Men

In 2006, the Centers for Disease Control and Prevention (CDC) revised its HIV testing recommendations in health care settings. Current guidelines recommend that all patients aged 13 to 64 years be screened for HIV as part of routine medical care. Additionally, the CDC recommends that persons at high risk for HIV infection should be screened for HIV at least annually. Primary care providers in clinical settings are seen as important providers of HIV prevention services and will be critical to expand HIV testing rates, particularly among populations such as men who have sex with men (MSM). This article reports on results from a multifaceted study that combined qualitative and quantitative methods and targeted both MSM and primary care providers. Through a quantitative survey with 709 MSM in Wisconsin, we explored the relationship between having a primary care provider, risk behavior, and HIV testing patterns. In the qualitative portion of the study, we conducted structured interviews with 7 clinic medical directors to explore the acceptability and feasibility of increased HIV testing in clinical setting strategy among primary care providers. Consistent with previous research, the results of this study indicate that primary care providers can play a significant role in encouraging and facilitating annual routine testing as a standard of care for high-risk MSM. This article offers policy and practice recommendations based on these findings.
Regenerative load emulator with battery charging for evaluation of energy management in microgrid with distributed renewable sources

Load emulation is the technique by which, different types of load characteristics can be realized without using the actual load modules. This is very useful for testing of power supply equipment under various load conditions. Further the technique of load emulation can be applied for micro grid design and power flow studies under various load conditions. A battery charger based regenerative load emulation technique is explained here which can be applied for emulated micro grids. The design is done in such a way that the system can emulate pure resistive, inductive and capacitive loads and regenerate the in phase power to store in battery. The stored energy can be further inverted and supplied back to the grid, thereby making the system regenerative in nature. The simulation results for the proposed load emulation technique, along with the application of the same in micro grids are presented in detail. The results show the required emulator waveforms and energy stored in to the battery.
CENTRAL MOMENTS AND PROBABILITY DISTRIBUTION OF COLLESS'S COEFFICIENT OF TREE IMBALANCE

The great increase in the number of phylogenetic studies of a wide variety of organisms in recent decades has focused considerable attention on the balance of phylogenetic trees—the degree to which sister clades within a tree tend to be of equal size—for at least two reasons: (1) the degree of balance of a tree may affect the accuracy of estimates of it; (2) the degree of balance, or imbalance, of a tree may reveal something about the macroevolutionary processes that produced it. In particular, variation among lineages in rates of speciation or extinction is expected to produce trees that are less balanced than those that result from phylogenetic evolution in which each extant species of a group has the same probability of speciation or extinction. Several coefficients for measuring the balance or imbalance of phylogenetic trees have been proposed. I focused on Colless's coefficient of imbalance (7) for its mathematical tractability and ease of interpretation. Earlier work on this statistic produced exact methods only for calculating the expected value. In those studies, the variance and confidence limits, which are necessary for testing the departure of observed values of I from the expected, were estimated by Monte Carlo simulation. I developed recursion equations that allow exact calculation of the mean, variance, skewness, and complete probability distribution of I for two different probability‐generating models for bifurcating tree shapes. The Equal‐Rates Markov (ERM) model assumes that trees grow by the random speciation and extinction of extant species, with all species that are extant at a given time having the same probability of speciation or extinction. The Equal Probability (EP) model assumes that all possible labeled trees for a given number of terminal taxa have the same probability of occurring. Examples illustrate how these theoretically derived probabilities and parameters may be used to test whether the evolution of a monophyletic group or set of monophyletic groups has proceeded according to a Markov model with equal rates of speciation and extinction among species, that is, whether there has been significant variation among lineages in expected rates of speciation or extinction.
Multiplex PCR reaction for the detection and identification of immunoglobulin kappa deleting element rearrangements in B‐lineage leukaemias

Immunoglobulin kappa (Igκ) gene recombinations can be used — similarly to IgH rearrangements — as clonal markers in B‐lineage leukaemias. Based on the extensive junctional diversity, these rearrangements represent valuable targets for the analysis of minimal residual disease (MRD). In order to provide a simple method for the rapid detection of leukaemia clone‐specific kappa deleting element (Kde) mediated rearrangements, we developed a multiplex PCR reaction that is able to amplify the five most frequent rearrangements in one tube. Position of the amplimers were chosen to enable identification of the involved segments according to the size of the PCR product. This method was tested on 101 B‐lineage leukaemias (71 childhood B‐cell precursor acute lymphoblastic leukaemias (BCP ALL) and 30 chronic lymphocytic leukaemias (CLL)). 39 and 22 Kde rearrangements could be readily detected in 30 (44%) BCP ALL and 22 (56%) CLL, respectively. 36% of the Kde rearrangements in BCP ALL and 45% in CLL were intron recombination signal sequence (RSS)‐Kde rearrangements. The other Kde rearrangements involved the Vκ families: VκI in 36% and 50%, VκII in 32% and 16.7%, VκIII in 24% and 25%, and VκIV in 8% and 8.3% in BCP ALL and CLL, respectively. The sensitivity of the multiplex system was 10−2–10−3. We compared this multiplex PCR assay with multiple single PCR reactions using different sets of primer combinations. Thereby the number and types of rearrangements were confirmed in all cases. Clonality of rearrangements was proven by sequence analysis. Our data show that by this method clonal Kde rearrangements were rapidly detected and precisely identified.
Gonococci with mutations to low-level penicillin resistance exhibit increased sensitivity to the oxygen-independent bactericidal activity of human polymorphonuclear leukocyte granule extracts

Gonococci which cause disseminated gonococcal infection are nearly always highly penicillin sensitive, in contrast to many isolates causing uncomplicated gonorrhea. We questioned whether any of the known chromosomal mutations to low-level penicillin resistance might adversely affect virulence. The penA2 locus is known to result in low-level resistance to penicillins, whereas mtr-2 results in nonspecific resistance to a variety of antimicrobial agents. We found that the penA2 and mtr-2 mutations each markedly increased sensitivity of strain FA19 to oxygen-independent killing by human polymorphonuclear leukocyte mixed or isolated azurophilic granule extracts. The penA2 and mtr-2 mutations had no effect on sensitivity to serum antibody and complement. Isogenic opaque or transparent variants of several strains of Neisseria gonorrhoeae were equally resistant to human polymorphonuclear leukocyte mixed granule extract bactericidal systems. There were also no differences in susceptibility of piliated type 1 and nonpiliated type 4 variants to human polymorphonuclear leukocyte mixed granule extracts. Since the penA2 and mtr-2 loci are known to increase the degree of cross-linking of cell wall peptidoglycan, the structure of peptidoglycan apparently affects sensitivity to killing by one or more polymorphonuclear leukocyte azurophilic granule extract bactericidal systems. These observations might explain why gonococci with mutations similar to penA2 and mtr-2 are almost never isolated from patients with disseminated gonococcal infection.
BEYOND REINFORCEMENT: THE EVOLUTION OF PREMATING ISOLATION BY DIRECT SELECTION ON PREFERENCES AND POSTMATING, PREZYGOTIC INCOMPATIBILITIES

Abstract The evolution of premating isolation after secondary contact is primarily considered in the guise of reinforcement, which relies on low hybrid fitness as the driving force for mating preference divergence. Here I consider two additional forces that may play a substantial role in the adaptive evolution of premating isolation, direct selection on preferences and indirect selection against postmating, prezygotic incompatibilities. First, I argue that a combination of ecological character displacement and sensory bias can cause direct selection on preferences that results in the pattern of reproductive character displacement. Both analytical and numerical methods are then used to demonstrate that, as expected from work in single populations, such direct selection will easily overwhelm indirect selection due to low hybrid fitness as the primary determinant of preference evolution. Second, postmating, prezygotic incompatibilities are presented as a driving force in the evolution of premating isolation. Two classes of these mechanisms, those increasing female mortality after mating but before producing offspring and those reducing female fertility, are shown to be identical in their effects on preference divergence. Analytical and numerical techniques are then used to demonstrate that postmating, prezygotic factors may place strong selection on preference divergence. These selective forces are shown to be comparable if not greater than those produced by the low fitness of hybrids. Corresponding Editor: M. Whitlock
Tonotopic variations of calcium signalling in turtle auditory hair cells

1 Turtle cochlear hair cells are electrically tuned by a voltage‐dependent Ca2+ current and a Ca2+‐dependent K+ current (IBK(Ca)). The effects of intracellular calcium buffering on electrical tuning were studied in hair cells at apical and basal cochlear locations tuned to 100 and 300 Hz, respectively. 2 Increasing the intracellular BAPTA concentration changed the hair cell's resonant frequency little, but optimized tuning at more depolarized membrane potentials due to a positive shift in the half‐activation voltage (V½) of the IBK(Ca). 3 The shift in V½ depended similarly on BAPTA concentration in basal and apical hair cells despite a 2·4‐fold difference in the size of the Ca2+ current at the two positions. The Ca2+ current amplitude increased exponentially with distance along the cochlea. 4 Comparison of V½ values and tuning properties using different BAPTA concentrations with values measured in perforated‐patch recordings gave the endogenous calcium buffer as equivalent to 0·21 mM BAPTA in low‐frequency cells, and 0·46 mM BAPTA in high‐frequency cells. 5 High conductance Ca2+‐activated K+ (BKCa) channels recorded in inside‐out membrane patches were 2‐fold less Ca2+ sensitive in high‐frequency than in low‐frequency cells. 6 Confocal Ca2+ imaging using the fluorescent indicator Calcium Green‐1 revealed about twice as many hotspots of Ca2+ entry during depolarization in high‐frequency compared to low‐frequency hair cells. 7 We suggest that each BKCa channel is gated by Ca2+ entry through a few nearby Ca2+ channels, and that Ca2+ and BKCa channels occupy, at constant channel density, a greater fraction of the membrane area in high‐frequency cells than in low‐frequency cells.
Exercise‐induced overexpression of key regulatory proteins involved in glucose uptake and metabolism in tetraplegic persons: molecular mechanism for improved glucose homeostasis

Complete spinal cord lesion leads to profound metabolic abnormalities and striking changes in muscle morphology. Here we assess the effects of electrically stimulated leg cycling (ESLC) on whole body insulin sensitivity, skeletal muscle glucose metabolism, and muscle fiber morphology in five tetraplegic subjects with complete C5‐C7 lesions. Physical training (seven ESLC sessions/wk for 8 wk) increased whole body insulin‐stimulated glucose uptake by 33±13%, concomitant with a 2.1‐fold increase in insulin‐stimulated (100 µU/ml) 3‐O‐methylglucose transport in isolated vastus lateralis muscle. Physical training led to a marked increase in protein expression of GLUT4 (378±85%), glycogen synthase (526±146%), and hexokinase II (204±47%) in vastus lateralis muscle, whereas phosphofructokinase expression (282±97%) was not significantly changed. Hexokinase II activity was significantly increased, whereas activity of phosphofructokinase, glycogen synthase, and citrate synthase was not changed after training. Muscle fiber type distribution and fiber area were markedly altered compared to able‐bodied subjects before ESLC training, with no change noted in either parameter after ECSL training. In conclusion, muscle contraction improves insulin action on whole body and cellular glucose uptake in cervical cord‐injured persons through a major increase in protein expression of key genes involved in the regulation of glucose metabolism. Furthermore, improvements in insulin action on glucose metabolism are independent of changes in muscle fiber type distribution. —Hjeltnes, N. , Galuska, D. , Björnholm, M. , Aksnes, A. ‐K. , Lannem, A. , Zierath, J. R. , Wallberg‐Henriksson, H. Exercise‐induced overexpression of key regulatory proteins involved in glucose uptake and metabolism in tetraplegic persons: molecular mechanism for improved glucose homeostasis. FASEB J. 12, 1701‐1712 (1998)
Extended behavior trees for quick definition of flexible robotic tasks

The requirement of flexibility in the modern industries demands robots that can be efficiently and quickly adapted to different tasks. A way to achieve such a flexible programming paradigm is to instruct robots with task goals and leave planning algorithms to deduct the correct sequence of actions to use in the specific context. A common approach is to connect the skills that realize a semantically defined operation in the planning domain — such as picking or placing an object — to specific executable functions. As a result the skills are treated as independent components, which results into suboptimal execution. In this paper we present an approach where the execution procedures and the planning domain are specified at the same time using solely extended Behavior Trees (eBT), a model formalized and discussed in this paper. At run-time, the robot can use the more abstract skills to plan a sequence using a PDDL planner, expand the sequence into a hierarchical tree, and re-organize it to optimize the time of execution and the use of resources. The optimization is demonstrated on a kitting operation in both simulation and lab environment, showing up to 20% save in the final execution time.
Nef induces apoptosis by activating JNK signaling pathway and inhibits NF-κB-dependent immune responses in Drosophila

The human immunodeficiency virus type 1 (HIV-1) nef gene encodes a 27-kDa protein that plays a crucial role during AIDS pathogenesis, but its exact functional mechanism has not been fully elucidated and remains controversial. The present study illuminated the in vivo functions of Nef using Drosophila, in which genetic analyses can be conveniently conducted. Using Drosophila transgenic lines for wild-type Nef, we demonstrated that Nef is not involved in the regulation of cell proliferation but rather specifically induces caspase-dependent apoptosis in wings in a cell-autonomous manner. Interestingly, myristoylation-defective Nef completely failed to induce the apoptotic wing phenotypes, consistent with previous reports demonstrating a crucial role for membrane localization of Nef in vivo. Further genetic and immunohistochemical studies revealed that Nef-dependent JNK activation is responsible for apoptosis. Furthermore, we found that ectopic expression of Nef inhibits Drosophila innate immune responses including Relish NF-κB activation with subsequent induction of an antimicrobial peptide, diptericin. The in vivo functions of Nef in Drosophila are highly consistent with those found in mammals and so we propose that Nef regulates evolutionarily highly conserved signaling molecules of the JNK and NF-κB signaling pathways at the plasma membrane, and consequently modulates apoptosis and immune responses in HIV target cells.
Recognition of the Lck tyrosine kinase as a tumor antigen by cytotoxic T lymphocytes of cancer patients with distant metastases

The Lck protein (p56lck), a src family tyrosine kinase that is essential for T cell development and function, is aberrantly expressed in metastatic colon cancers. p56lck seems to facilitate the malignant transformation of epithelial cells through initiation of anchorage‐independent proliferation. We demonstrate that the lck gene encodes antigenic epitopes recognized by the HLA class I‐restricted and tumor‐specific CTL of metastatic cancer patients. Lck peptides augmented CTL activity in peripheral blood mononuclear cells (PBMC) of colon and other epithelial cancer patients with distant metastases, but not those without distant metastases. CTL precursors recognizing the Lck peptide were identified in freshly prepared PBMC of patients with distant metastases, and their frequency was significantly augmented by stimulation with the peptide. Thus, Lck peptides could be useful in developing a specific immunotherapy for cancer patients with distant metastases.
Direct Binding of Lgl2 to LGN during Mitosis and Its Requirement for Normal Cell Division*

The Drosophila tumor suppressor protein lethal (2) giant larvae (l(2)gl) is involved in asymmetric cell division during development and epithelial cell polarity through interaction with the aPKC·Par-6 complex. We showed here that Lgl2, a mammalian homolog of l(2)gl, directly bound to LGN, a mammalian homolog of Partner of inscuteable in HEK293 cells. The C-terminal tail of Lgl2 bound to LGN with a Kd value of about 56 nm. Endogenous Lgl2 formed a complex with aPKC, Par-6, and LGN. This complex formation was enhanced in metaphase of the synchronized cells by treatment with thymidine and nocodazole. Immunofluorescence staining of the complex was the strongest at the cell periphery of the metaphase cells. Overexpression of the C-terminal tail of Lgl2 induced mis-localization of the nuclear mitotic apparatus protein NuMA and disorganization of the mitotic spindle during mitosis, eventually causing formation of multiple micronuclei. Knockdown of endogenous Lgl (Lgl1 and Lgl2) also induced disorganization of the mitotic spindle, thereby causing formation of multiple micronuclei. The binding between Lgl2 and LGN played a role in the mitotic spindle organization through regulating formation of the LGN·NuMA complex. These results indicate that Lgl2 forms a Lgl2·Par-6·aPKC·LGN complex, which responds to mitotic signaling to establish normal cell division.
Message Framing and Mammography Screening: A Theory-Driven Intervention

Abstract Although the rising incidence of breast cancer has prompted a surge of intervention strategies aimed at increasing women's use of mammography screening, the majority of patient-directed interventions have not been driven by relevant theoretical work on persuasive health communication. The authors evaluated an intervention derived from prospect theory that was designed to increase women's adherence to recommendations for annual mammography screening. They sent 1 of 3 reminder letters (positive frame, negative frame, or standard hospital prompt) to 929 randomly selected women who were due for mammography screening and had been identified as having either a positive or negative family history of breast cancer. The primary hypothesis that women with a positive history would be more responsive to negatively framed messages, whereas women with a negative history would be more responsive to positively framed letters, was not confirmed. The lack of support for predictions derived from prospect theory raises important questions about the generalizability of laboratory research to natural settings.
METAMORFOSIS “ILLAT HUKUM” DALAM SAD ADZ-DZARI’AH DAN FATH ADZ-DZARIAH (SEBUAH KAJIAN PERBANDINGAN)

Sad Adz-Dzariah is a breakthrough method produced by the scholars of Usul Fikih to protecting and keeping the human being as Mukallaf falling or rubbing on damage or mafsadah by closing and blocking all means, tools and wasilah that will be used for some action. But as a result of the development of life, there is another aspect that should be of concern as opposed also to avoiding damage or Mafsadah, namely the realization of the benefit or Jalbu al-Maslahah, by opening and allowing to use the means, tools and or wasilah that will be used for some action, by another method of ijtihad Fath Adz-Dzariah. So that the next concern is on the logical link or 'Illat Law must also be seen again for use, so then the transition from the Sad Adz-Dzariah Method to the Fath Adz-Dzariah Method can be done.Kata Kunci: Sad Adz-Dzariah, Fath Adz-Dzariah, sarana, alat dan wasilah