Miracle-dz/UniFAR-FTUs huggingface dataset:
Physical Activity, Injurious Falls, and Physical Function in Aging: An Umbrella Review

Purpose To review and update the evidence of the relationship between physical activity, risk of fall-related injury, and physical function in community-dwelling older people that was presented in the 2018 Physical Activity Guidelines Advisory Committee Scientific Report (PAGAC Report). Methods Duplicate independent screenings of 1415 systematic reviews and meta-analyses published between 2006 and 2016 identified from PubMed®, Cochrane Library, and CINAHL databases yielded 111 articles used for the PAGAC Report. The PAGAC Aging Subcommittee members graded scientific evidence strength based upon a five-criteria rubric and assigned one of four grades: strong, moderate, limited, or not assignable. An updated search of 368 articles published between January 2017 and March 2018 yielded 35 additional pertinent articles. Results Strong evidence demonstrated that physical activity reduced the risk of fall-related injuries by 32% to 40%, including severe falls requiring medical care or hospitalization. Strong evidence also supported that physical activity improved physical function and reduced the risk of age-related loss of physical function in an inverse graded manner among the general aging population, and improved physical function in older people with frailty and with Parkinson’s disease. Aerobic, muscle-strengthening, and/or multicomponent physical activity programs elicited the largest improvements in physical function in these same populations. Moderate evidence indicated that for older adults who sustained a hip fracture or stroke, extended exercise programs and mobility-oriented physical activity improved physical function. Conclusions Regular physical activity effectively helps older adults improve or delay the loss of physical function and mobility while reducing the risk of fall-related injuries. These important public health benefits underscore the importance of physical activity among older adults, especially those living with declining physical function and chronic health conditions.
Intein-mediated protein trans-splicing expands adeno-associated virus transfer capacity in the retina

Adeno-associated viral vectors reconstitute large proteins in the retina via intein-mediated protein trans-splicing. Leveraging naturally occurring splicing for gene therapy Retinal gene therapy using adeno-associated viral (AAV) vectors has been shown to be effective in a number of eye diseases. However, the limited capacity of AAV hampers the use of gene therapy for delivering large genes. Now, Tornabene et al. leveraged the properties of genetic elements called split inteins to deliver large genes using multiple vectors. In unicellular organisms, split inteins mediate the fusion of two separate peptides. Here, the authors were able to reconstitute large full-length proteins using split inteins in vitro in pig retina and human organoids and in vivo in two mouse models of inherited retinal diseases with high efficiency, obtaining therapeutic effects. Retinal gene therapy with adeno-associated viral (AAV) vectors holds promises for treating inherited and noninherited diseases of the eye. Although clinical data suggest that retinal gene therapy is safe and effective, delivery of large genes is hindered by the limited AAV cargo capacity. Protein trans-splicing mediated by split inteins is used by single-cell organisms to reconstitute proteins. Here, we show that delivery of multiple AAV vectors each encoding one of the fragments of target proteins flanked by short split inteins results in protein trans-splicing and full-length protein reconstitution in the retina of mice and pigs and in human retinal organoids. The reconstitution of large therapeutic proteins using this approach improved the phenotype of two mouse models of inherited retinal diseases. Our data support the use of split intein–mediated protein trans-splicing in combination with AAV subretinal delivery for gene therapy of inherited blindness due to mutations in large genes.
Effect of microencapsulation methods on the survival of freeze-dried Bifidobacterium bifidum

Six kinds of Bifidobacterium bifidum microcapsules were prepared by extrusion methods, emulsion methods and coacervation methods. Effects of preparation methods on the survival of encapsulated B. bifidum were examined. Results showed that microcapsules prepared by emulsion method with alginate and chitosan exhibited the best protection for B. bifidum. The diameter was 10–20 µm, encapsulation efficiency was 90.36% and the live cell amount was 3.01 × 109 cfu/g after freeze-drying. Encapsulated cells exhibited significantly higher resistance to artificial gastrointestinal juice and the cell numbers were above 109 cfu/g after exposure to simulated gastric (pH 1.2) and bile salt (1%, w/v). Cell numbers of microencapsulated B. bifidum was 8.61 × 108 cfu/g after storage at 37°C, relative humidity 60%–65% for 3 months. Results indicated microcapsules prepared with alginate and chitosan by emulsion method could successfully protect B. bifidum against adverse conditions and it might be useful in the delivery of probiotic cultures as a functional food.
Empirical Insights of Individual Website Adjustments for People with Dyslexia

The existing body of knowledge reveals that customisable websites may lead to an increase in accessibility and usability for people with disabilities. In this way, the main goal of this research was to investigate how people with dyslexia respond to a customised version of a website in terms of its effectiveness, efficiency, satisfaction and suitability when compared to the default version of the website. The customisation of the investigated website was enabled with the aid of integrated assistive technology that offers people with dyslexia the opportunity to adjust a website themselves in accordance with their individual needs, demands and preferences. They can do this by changing the parameters, such as font size, font type and contrast between the background and text. The answers to the research questions were obtained with complementary research methods and techniques, including formal usability testing, thinking aloud protocol, log analyses, questionnaires and interviews. The empirical results show that participants experienced more issues when interacting with the default website, and they enjoyed more benefits when using the customised website. Too much information on the screen, not enough graphic elements, issues with visual appearance and inappropriately presented information were identified as the most common issues when interacting with the default website. When using the customised website, all participants agreed on a better user experience and, as the majority of them reported, this was due to appropriate contrast and font size. Additionally, the majority of participants also expressed desire to use the individual website adjustments regularly in the future. The conclusions of this investigation are that the individual website adjustments used in this research can not only help to minimise issues, but also eliminate challenges that people with dyslexia have when interacting with a website. Therefore, the primary contributions of this research are the empirical insights of interaction with both the default and customised version of the website for people with dyslexia. Furthermore, this research also has three secondary contributions: (1) detailed presentation and application the general usability evaluation procedure to a specific target group (people with dyslexia); (2) recommendations to adapt the usability evaluation methods for people with dyslexia; and (3) the usage of quantitative measurement instruments for the evaluation of a website’s usability and suitability for people with dyslexia.
Rationing of Nursing Care and Its Relationship with Nurse Staffing and Patient Outcomes: The Mediation Effect Tested by Structural Equation Modeling

Purpose: The purpose of this study is to test the mediation effect of rationing of nursing care (RONC) and the relationship this has between nurse staffing and patient outcomes. Methods: The analytic sample included 7802 nurse surveys and 5430 patient surveys. Three patient outcome indicators, nurse staffing, RONC, and confounding factors were considered in the model pathways. Results: The hypothesized model was shown to be statistically significant. In the model, nurses who were in the units with lower nurse-to-patient ratios reported higher scores on RONC, which meant that an increased level of withheld nursing care or a failure to carry out nursing duties was apparent. Nurses who reported a higher score on RONC, scored poorly on the quality assessment and were more frequently involved in patient adverse events. Nurse staffing influenced quality assessments and patient adverse events through RONC. In units with poorer nurse-reported quality assessments or more frequently patient adverse events, patient-reported dissatisfaction scores were higher. Conclusions: The results suggest that a lack of nurse staffing leads to RONC, which leads to poorer patient outcomes. These results are seen when considering the evaluations completed by both nurses and patients. The relationship between staffing numbers and patient outcomes explains the mediating role of RONC.
Screening a Resource of Recombinant Protein Fragments for Targeted Proteomics

The availability of proteomics resources hosting protein and peptide standards, as well as the data describing their analytical performances, will continue to enhance our current capabilities to develop targeted proteomics methods for quantitative biology. This study describes the analysis of a resource of 26,840 individually purified recombinant protein fragments corresponding to more than 16,000 human protein-coding genes. The resource was screened to identify proteotypic peptides suitable for targeted proteomics efforts and we report LC-MS/MS assay coordinates for more than 25,000 proteotypic peptides, corresponding to more than 10,000 unique proteins. Additionally, peptide formation and digestion kinetics were, for a subset of the standards, monitored using a time-course protocol involving parallel digestion of isotope-labelled recombinant protein standards and endogenous human plasma proteins. We show that the strategy by adding isotope-labelled recombinant proteins prior to trypsin digestion enables short digestion protocols (≤60 min) with robust quantitative precision. In a proof-of-concept study, we quantified 23 proteins in human plasma using assay parameters defined in our study and used the standards to describe distinct clusters of individuals linked to different levels of LPA, APOE, SERPINA5 and TFRC. In summary, we describe the use and utility of a resource of recombinant proteins to identify proteotypic peptides useful for targeted proteomics assay development.
Detecting middle ear fluid using smartphones

To diagnose otitis media, a smartphone system detects middle ear fluid using the built-in microphone and speakers. Hearing an ear infection? Ear infections are typically diagnosed using specialized equipment to assess eardrum mobility: The presence of fluid in the middle ear, indicative of likely ear infection, limits eardrum mobility. Chan et al. developed a smartphone system to detect middle ear fluid that uses the microphone and speaker of a phone to emit sound and analyze its reflection (echo) from the eardrum. The smartphone system outperformed a commercial acoustic reflectometry system in detecting middle ear fluid in 98 pediatric patient ears, and the system could be easily operated by patient parents without formal medical training. This proof-of-concept screening tool could help aid in the diagnosis of ear infections. The presence of middle ear fluid is a key diagnostic marker for two of the most common pediatric ear diseases: acute otitis media and otitis media with effusion. We present an accessible solution that uses speakers and microphones within existing smartphones to detect middle ear fluid by assessing eardrum mobility. We conducted a clinical study on 98 patient ears at a pediatric surgical center. Using leave-one-out cross-validation to estimate performance on unseen data, we obtained an area under the curve (AUC) of 0.898 for the smartphone-based machine learning algorithm. In comparison, commercial acoustic reflectometry, which requires custom hardware, achieved an AUC of 0.776. Furthermore, we achieved 85% sensitivity and 82% specificity, comparable to published performance measures for tympanometry and pneumatic otoscopy. Similar results were obtained when testing across multiple smartphone platforms. Parents of pediatric patients (n = 25 ears) demonstrated similar performance to trained clinicians when using the smartphone-based system. These results demonstrate the potential for a smartphone to be a low-barrier and effective screening tool for detecting the presence of middle ear fluid.
Topic Modeling the Hàn diăn Ancient Classics

Ancient Chinese texts present an area of enormous challenge and opportunity for humanities scholars interested in exploiting computational methods to assist in the development of new insights and interpretations of culturally significant materials. In this paper we describe a collaborative effort between Indiana University and Xi'an Jiaotong University to support exploration and interpretation of a digital corpus of over 18,000 ancient Chinese documents, which we refer to as the "Handian" ancient classics corpus (H\`an di\u{a}n g\u{u} j\'i, i.e, the "Han canon" or "Chinese classics"). It contains classics of ancient Chinese philosophy, documents of historical and biographical significance, and literary works. We begin by describing the Digital Humanities context of this joint project, and the advances in humanities computing that made this project feasible. We describe the corpus and introduce our application of probabilistic topic modeling to this corpus, with attention to the particular challenges posed by modeling ancient Chinese documents. We give a specific example of how the software we have developed can be used to aid discovery and interpretation of themes in the corpus. We outline more advanced forms of computer-aided interpretation that are also made possible by the programming interface provided by our system, and the general implications of these methods for understanding the nature of meaning in these texts.
Brain targeting of resveratrol through intranasal lipid vesicles labelled with gold nanoparticles: in vivo evaluation and bioaccumulation investigation using computed tomography and histopathological examination

Abstract Resveratrol is a promising neuroprotective agent against neurodegenerative disorders such as Alzheimer's disease. Resveratrol-loaded transferosomes and nanoemulsions were developed and labelled with gold nanoparticles (GNPs). The water maze test was utilised to identify the effect on spatial memory recovery. The treated rats were examined for cellular uptake and bioaccumulation of drug in the brain using computed tomography (CT) and histopathological examination utilising GNPs as a biomarker. Compared with nanoemulsions, transferosomes displayed higher permeation of up to 81.29 ± 2.64% and higher fluorescence intensity with p < . 05. Transferosomes significantly enhanced behavioural acquisition and spatial memory function in the amnesic rats compared with both the nanoemulsion formulation and the pure drug. CT effectively demonstrated the accumulation of GNPs in the brains of all treated rats, while superior accumulation of GNPs was observed in the rats that received the transferosome formulation. The histopathology also demonstrated GNP accumulation in the nuclei and cytoplasm in the brain tissues of both the transferosome- and nanoemulsion-treated groups. Therefore, the developed transferosomes may be considered as a well-designed brain targeting system that might further be applied for targeting many drugs to be used in the treatment of central nervous system diseases.
The Relationship between Change of Direction Tests in Elite Youth Soccer Players

Change of direction (COD) is a performance-limiting factor in team sports. However, there are no exact definitions describing which physical abilities limit COD performance in soccer. Nevertheless, different COD tests are used or have been recommended as being equally effective in the professional practice of measuring COD performance. Therefore, the aim of this study was to evaluate the relationship between different COD tests, and to test the independence and generalizability of these COD tests in soccer. As such, 27 elite youth soccer players were randomly recruited and were tested in different COD tests (i.e., Illinois agility test (IAT), T agility test (TT), 505 agility test (505), Gewandtheitslauf (GewT), triangle test (Tri-t), and square test (SQT)). Bivariate Pearson correlation analysis was used to assess the relationships between the COD tests. The Benjamini–Hochberg method was used to control for the false discovery rate of the study at 0.05. This investigation calculated explained variances of 10% to 55% between performances in the different COD tests. This suggested that the tests covered different aspects or task-specific characteristics of the COD. Therefore, coaches and sport scientists should review and select different tests with a logical validity, based on the requirement profiles of the corresponding sport.
A Randomized Controlled Trial of mHealth Mindfulness Intervention for Cancer Patients and Informal Cancer Caregivers: A Feasibility Study Within an Integrated Health Care Delivery System

Purpose: To assess feasibility and preliminary efficacy of a mobile/online-based (mHealth) mindfulness intervention for cancer patients and their caregivers to reduce distress and improve quality of life (QoL). Material and Methods: Two-arm randomized controlled trial within Kaiser Permanente Northern California targeting cancer patients who received chemotherapy and their informal caregivers. The intervention group received a commercially available mindfulness program for 8 weeks. The wait-list control group received usual care. We assessed feasibility using retention and adherence rates and obtained participant-reported data on distress, QoL, sleep, mindfulness, and posttraumatic growth before and immediately after the intervention. Results: Ninety-seven patients (median age 59 years; female 69%; 65% whites) and 31 caregivers (median age 63 years; female 58%; 77% whites) were randomized. Among randomized participants, 74% of the patients and 84% of the caregivers completed the study. Among those in the intervention arm who initiated the mindfulness program, 65% practiced at least 50% of the days during the intervention period. We observed significantly greater improvement in QoL among patients in the intervention arm compared with controls. Caregivers in the intervention group experienced increased mindfulness compared with controls. Participants appreciated the convenience of the intervention and the mindfulness skills they obtained from the program. Conclusion: We demonstrated the feasibility of conducting a randomized trial of an mHealth mindfulness intervention for cancer patients and their informal caregivers. Results from fully powered efficacy trials would inform the potential for clinicians to use this scalable intervention to help improve QoL of those affected by cancer and their caregivers.
Reduced graft function (with or without dialysis) vs immediate graft function--a comparison of long-term renal allograft survival.

BACKGROUND Delayed graft function (DGF) is a common complication in cadaveric kidney transplants affecting graft outcome. However, the incidence of DGF differs widely between centres as its definition is very variable. The purpose of this study was to define a parameter for DGF and immediate graft function (IGF) and to compare the graft outcome between these groups at our centre. METHODS The renal allograft function of 972 first cadaveric transplants performed between 1990 and 2001 in the Republic of Ireland was examined. The DGF and IGF were defined by a creatinine reduction ratio (CRR) between time 0 of transplantation and day 7 post-transplantation of <70 and >70%, respectively. Recipients with reduced graft function (DGF) not requiring dialysis were defined as slow graft function (SGF) patients. The serum creatinine at 3 months, 6 months, 1, 2 and 5 years after transplantation was compared between these groups of recipients. The graft survival rates at 1, 3 and 5 years and the graft half-life for DGF, SGF and IGF recipients were also assessed. RESULTS Of the 972 renal transplant recipients, DGF was seen in 102 (10.5%) patients, SGF in 202 (20.8%) recipients and IGF in 668 (68.7%) patients. Serum creatinine levels were significantly different between the three groups at 3 and 6 months, 1, 2 and 5 years. Graft survival at 5 years for the DGF patients was 48.5%, 60.5% for SGF recipients and 75% for IGF patients with graft half-life of 4.9, 8.7 and 10.5 years, respectively. CONCLUSION This study has shown that the CRR at day 7 correlates with renal function up to 5 years post-transplantation and with long-term graft survival. We have also demonstrated that amongst patients with reduced graft function after transplantation, two groups with significantly different outcomes exist.
Post‐traumatic intrasplenic pseudoaneurysms with delayed rupture: Color Doppler sonographic and CT findings

Post‐traumatic intrasplenic pseudoaneurysms are very rare in children. Since pseudoaneurysms may expand a splenic hematoma and cause delayed splenic rupture, early diagnosis and treatment are crucial. In this report, we describe the case of a 12‐year‐old boy with a delayed splenic rupture caused by a splenic hematoma containing 2 pseudoaneurysms. Abdominal sonography showed free intraperitoneal fluid and a mildly enlarged spleen with a large heterogeneous area occupying the upper half of the organ. Two anechoic lesions (15 and 4 mm) were seen inside the hematoma near the splenic hilum. Color Doppler sonography demonstrated turbulent arterial flow within the lesions, suggesting pseudoaneurysms. On CT, the lesions enhanced simultaneously with the splenic artery in the arterial phase of contrast enhancement. CT also showed an intrasplenic arterial branch leading to the larger of the 2 pseudoaneurysms. © 2001 John Wiley & Sons, Inc. J Clin Ultrasound 29:102–104, 2001.
Molecular Docking studies and in-silico ADMET Screening of Some novel Oxazine substituted 9-Anilinoacridines as Topoisomerase II Inhibitors

Introduction: In general, 9-aminoacridine derivatives are inhibiting DNA topoisomerase II (topoII) because of their strong activity due to the ability of acridine nucleus to intercalate into DNA base pair. To get insight of the intermolecular interactions, the molecular docking studies are performed at active site of topoisomerase II. Aim: In this study, an attempt is made for identification of potential ligands from oxazine substituted 9-anilino acridines targeted against topoisomerase-II (1ZXM) using molecular modelling and docking studies by using Schrodinger suit-2012 Maestro 9.3 version. Insilco ADMET screening also performed by qikprop module of Schrodinger suit. Results: The relative binding affinity of the designed compounds towards topoisomerase-II (1ZXM) was selected on the basis of docking score, GLIDE score and interaction patterns. Several compounds showed strong hydrogen bonding interactions with amino acid residues and their hydrophobic interactions and other parameters could also explain their potency to inhibit topoisomerase-II (1ZXM). The oxazine substituted 9-anilino acridine derivatives 1a1k have good binding affinity with Glide score in the range of -5.7 to -8.06 when compared with the standard ledacrine (-5.24). The ADMET screening of the designed compounds have almost all the properties of the compounds are within the recommended values. Conclusion: Hence, this study provides evidence for consideration of valuable ligands in oxazine substituted 9-anilino acridine derivatives as potential topoisomerase-II inhibitor and further in vitro and in vivo investigations may prove its therapeutic potential