Miracle-dz/UniFAR-FTUs huggingface dataset:
Cooperative attribute-based access control for enterprise computing system

In this paper we introduce a cooperative attribute-based access control mechanism, which is specifically designed for enterprise computing systems. In our system, users are divided into different groups and they are affiliated with different attributes. Only members from the same group can combine their signing keys to form the signing key of a larger union set of attributes, but users from different groups cannot make it. With the union of the attributes, users can generate a signature which can be used to grant access right to the enterprise cloud system. The applications range from private cloud of a small and medium enterprise (SME) to a large public cloud of electronic healthcare system. We give an efficient design of this mechanism, formally prove its security and implement the prototype of our scheme.
A versatile vector system for generating recombinant EGFP-tagged proteins in yeast

This paper reports a versatile egfp-tagged pFL61-based expression vector system which allows the production on yeast of homo- and heterologous proteins fused with the Enhanced Green Fluorescent Protein (EGFP) at the C-terminus. This expression system, which involves a fluorescent protein, readily allows both to verify the expression and to localize the protein in the yeast cell. The vector carries a Not I site upstream the first codon of the egfp gene. The yeast cells harbouring this plasmid emit a feeble emission compared to the fluorescence expected. Was then investigated the effect of the Not I site, located very close to the start codon, on the expression of the reporter egfp gene using northern and western blotting, fluorescence microscopy and flow cytometry. Data indicated that this palindromic site could hide the start codon so as to negatively affect translation. This aspect confers to the proposed expression system an advantage in distinguishing clones after transformation.
Simulation of 4H-SiC Trench Junction Barrier Schottky Diodes with High-k Dielectrics

In this paper, a novel 4H-SiC trench JBS (TkJBS) with high-k semi-insulating polycrystalline silicon (SIPOS) is proposed. By adopting a new high-k dielectrics-trench structure, the peak electric field at the corner of P+ region (COP) in Silicon Carbide (SiC) can be weakened significantly, and the device breakdown voltage can be enhanced significantly. SiC TkJBS has a 60.7% higher breakdown voltage (BV) and a 110.6% higher Baliga’s figure of merit (BFOM) than the conventional SiC JBSs, and the surface electric field (Esf) of the SiC TkJBS is 0.73 MV/cm lower than it at breakdown voltages, resulting in a much lower tunneling current (Ir). The reverse recovery characteristics of SiC TkJBS are also slightly better than those of the SiC JBS. It is indicated that SiC TkJBS is a promising candidate for next-generation high power diodes.
A new approach for determination of moisture in paper insulation of in-situ power transformers by combining polarization-depolarization current and return voltage measurement results

Moisture content in an oil-paper insulation system of power transformer accelerates the aging process of cellulose that degrades insulation properties. As a result, dielectric response measurement data is also affected by moisture present in insulation. In time domain these measurements include Return Voltage Measurement and Polarization-Depolarization Current measurement. In addition it is a known fact that power transformer insulation consists of complex structures which is difficult to model in laboratory. Therefore, results obtained from samples prepared under controlled environment may not be applicable to real life power transformer. The present paper discusses a new approach to combine the results obtained from these two methods namely, Return Voltage Measurement and Polarization-Depolarization Current measurement for several power transformers, to find the relation between oil and paper moisture content that is relevant to insitu power transformers.
Gene Expression Profiling of Neu-induced Mammary Tumors from Transgenic Mice Reveals Genetic and Morphological Similarities to ErbB 2-expressing Human Breast Cancers 1

Numerous studies have shown that the overexpression and amplification of ErbB2/Neu are observed in 20–30% of patients afflicted with breast cancer. Furthermore, it has also been observed that the elevated expression of ErbB2/Neu also correlates with poor prognosis and clinical outcome. Given the prevalence of this disease, we sought to create mouse models that mimic the human condition. In this study, we compared two mouse models expressing activated neu under the control of the endogenous and mouse mammary tumor virus promoters. Although histologically similar, the latency and metastatic potential of these tumors are remarkably different. Gene expression profiling of tumor RNA from the two Neu mouse models revealed distinctive and nonoverlapping patterns of gene expression. Consistent with noninvasive nature of the mammary tumors induced by expression of neu under the endogenous promoter, these tumors expressed a number of markers characteristic of a highly differentiated state. In addition to these differences, these analyses revealed that in contrast to the mouse mammary tumor virus-based Neu model, the endogenous promoter tumors expressed elevated levels of two genes (Grb7 and Cab1) that are closely linked to ErbB2 and often coamplified in noninvasive ductal carcinoma in situ. Furthermore, this analysis has revealed several transcription factors that may be involved in ErbB2mediated tumorigenesis. Taken together, these results illustrate the similarity of the endogenously regulated Neu tumor model to the human disease.
The evolution of consent forms for research: a quarter century of changes.

The informed consent process in the research context typically relies heavily on information provided to potential research participants in a written consent form. Federal regulations governing research with humans list the elements of informa tion that consent forms must contain and indicate that there may be additional elements relevant to particular types of studies.1 Consent forms typically serve as the participants' only written explanation of the studies in which they have enrolled and as important documen tation for researchers and regulators of individuals' consent to participate in a study. Institutional review boards (IRBs) often pay considerable? and some would say excessive? attention to the content and wording of these forms.2 Despite the critical role of consent forms in the research context, there is good reason to believe that, as currently formulated, they are often far from ideal.3 Among the problems that have been documented are excessive length,4 complexity of wording that exceeds average reading levels,5 and problems with the accura cy with which key aspects of the studies are described.6 Numerous proposals have been made for improving consent forms,7 but there is little evidence that attempts at improvement have had a positive effect. Indeed, we have been unable to find sufficient data examining trends in consent forms over time to determine whether most of these concerns mentioned above have been adequately resolved. To our knowledge, the only avail able longitudinal data focus on changes in the length of forms, and the data suggest that things may be get ting worse instead of better. For instance, Baker and Taub demonstrated that the mean length of consent forms nearly doubled between 1975 and 1982.8 More recently, Beardsley and colleagues in Australia found
Optimization of a post-type spacer in a gas insulated system under three-dimensional conditions

This paper describes the optimization of a post-type spacer in a gas insulated system. The electric field calculation and the optimization have been performed in three-dimensional conditions. We have utilized the boundary element method to calculate the electric field distributions and the Marquardt method to improve the profile of the spacer so as to minimize the maximal electric field on the spacer surface. The optimization has been performed for spacers with cylindrical column, circular, and elliptical cross-sections, respectively. We have varied additional parameters, such as the permittivity of a spacer and the radius ratio of an inner conductor and a sheath. For elliptical cross-section spacers, the optimization has reduced the maximal value of the total field strength up to 35%, and the maximal value of the tangential field strength up to 56% compared with the respective maximal field without a spacer.
Notice of RetractionResearch On Wind Power Connected To Power Grid By Fractional Frequency Transmission System

On the base of study on the characteristic of the currently existing parallel operation model of wind power and the technology of fractional frequency transmission system (FFTS), wind power system connected to power grid via FFTS is presented in this paper. The system is modeled and simulated with MATLAB/SIMULINK. FFTS is suitable for low-frequency wind power, has help in reducing the wind turbine size and weight, and can reduce transmission line loss. Results show that the FFTS is an effective and potential way used in wind power system connected to power grid.
Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones.

Sixteen P1 and TAC clones assigned to Arabidopsis thaliana chromosome 5 were sequenced, and their sequence features were analyzed using various computer programs. The total length of the sequences determined was 1,013,767 bp. Together with the nucleotide sequences of 109 clones previously reported, the regions of chromosome 5 sequenced so far now total 9,072,622 bp, which presumably covers approximately one-third of the chromosome. A similarity search against the reported gene sequences predicted the presence of a total of 225 protein-coding genes and/or gene segments in the newly sequenced regions, indicating an average gene density of one gene per 4.5 kb. Introns were identified in 72.4% of the potential protein genes for which the entire gene structure was predicted, and the average number per gene and the average length of the introns were 3.3 and 163 bp, respectively. These sequence features are essentially identical to those in the previously reported sequences. The sequence data and gene information are available on the World Wide Web database KAOS (Kazusa Arabidopsis data Opening Site) at http://www.kazusa. or.jp/arabi/.
Indiscernibility degree of objects for evaluating simplicity of knowledge in the clustering procedure

The paper presents a novel, rough set-based clustering method that enables the evaluation of classification knowledge simplicity during the clustering procedure. The method iteratively refines equivalence relations so that they become a more simple set of relations that give adequate coarse classification to the objects. At each step of the iteration, the importance of the equivalence relation is evaluated on the basis of the newly introduced measure, indiscernibility degree. An indiscernibility degree is defined as a ratio of equivalence relations that classify the two objects into the same equivalence class. If an equivalence relation has the ability to discern two objects that have a high indiscernibility degree, a very fine classification is performed and then modified to regard them as indiscernible objects. The refinement is repeated, decreasing the threshold level of indiscernibility degree, and finally simple clusters can be obtained. Experimental results on the artificial data shows that iterative refinement of equivalence relation leads to successful generation of coarse clusters that can be represented by simple knowledge.
p53 Arg72Pro Polymorphism Predicts Survival Outcome in Lung Cancer Patients in Indian Population

In response to many forms of cellular stress, including DNA damage, the p53 protein functions to induce growth arrest, DNA repair, or apoptosis. Common allele variants in the TP53 gene modulate pathways of lung carcinogenesis and susceptibility to or prognosis of lung cancer. The prognostic role of the polymorphism was assessed in 422 subjects using PCR-RFLP. Logistic regression analysis showed a dominant presentation of Pro/Pro homozygotes in lung carcinoma population than in control population (OR = 2.1, P = 0.003). We further investigated the association of p53 codon 72 polymorphism with prognosis in 170 lung cancer patients. Kaplan-Meier survival analyses showed a significant difference in survival between p53 variant genotypes and overall survival (P = 0.02). Cox regression analysis showed p53 Arg72Pro heterozygous genotype was overall an independent prognostic factor (Risk ratio of death, 2.2; P = 0.02), suggesting Pro72Pro genotype to be a potential risk factor favoring the development of lung carcinoma and that Arg72Pro genotype is independently associated with a poorer prognosis of lung cancer.
Vaccine Perceptions Among Oregon Health Care Providers

Health care providers exert a significant influence on parental pediatric vaccination decisions. We conducted hour-long interviews with traditional and alternative health care providers in which we explored a range of associations between vaccination perceptions and practice. A key finding was that the Health Belief Model constructs of perceived susceptibility to and severity of either an illness or an adverse vaccine event partially explained health care provider (HCP) beliefs about the risks or benefits of vaccination, especially among alternative care providers. Low or high perceived susceptibility to a vaccine-preventable disease (VPD) or of the severity of a given VPD affects whether an HCP will promote or oppose pediatric vaccination recommendations. Beyond these perceptions, health and vaccination beliefs are affected by the contextual factors of personal experience, group norms, immunology beliefs, and beliefs about industry and government. Building powerful affective heuristics might be critical to balancing the forces that defeat good public health practices.
Production of Triterpenoid Sapogenins in Hairy Root Cultures of Silene vulgaris

Silene vulgaris (Moench) Garcke (Caryophyllaceae) is widely distributed in North America and contains bioactive oleanane-type saponins. In order to investigate in vitro production of triterpenoid saponins, hairy root cultures of S. vulgaris were established by infecting leaf explants with five strains of Agrobacterium rhizogenes (LBA9402, R1000, A4, 13333, and 15834). The A. rhizogenes strain LBA9402 had an infection of 100% frequency and induced the most hairy roots per plant. Methyl jasmonate (MeJA)-induced changes in triterpenoid saponins in S. vulgaris hairy roots were analyzed. Accumulation of segetalic acid and gypsogenic acid after MeJA treatment was 5-and 2-fold higher, respectively, than that of control root. We suggest that hairy root cultures of S. vulgaris could be an important alternative approach to the production of saponins.
Measurement of the electronic thermal conductance channels and heat capacity of graphene at low temperature

The ability to transport energy is a fundamental property of the two-dimensional Dirac fermions in graphene. Electronic thermal transport in this system is relatively unexplored and is expected to show unique fundamental properties and to play an important role in future applications of graphene, including opto-electronics, plasmonics, and ultra-sensitive bolometry. Here we present measurements of bipolar, electron-diffusion and electron-phonon thermal conductances, and infer the electronic specific heat, with a minimum value of 10 $k_{\rm{B}}$ ($10^{-22}$ JK$^{-1}$) per square micron. We test the validity of the Wiedemann-Franz law and find the Lorenz number equals $1.32\times(\pi^2/3)(k_{\rm{B}}/e)^2$. The electron-phonon thermal conductance has a temperature power law $T^2$ at high doping levels, and the coupling parameter is consistent with recent theory, indicating its enhancement by impurity scattering. We demonstrate control of the thermal conductance by electrical gating and by suppressing the diffusion channel using superconducting electrodes, which sets the stage for future graphene-based single microwave photon detection.
Measurement of cytoplasmic calcium concentration in the rods of wild‐type and transducin knock‐out mice

A 10 μm spot of argon laser light was focused onto the outer segments of intact mouse rods loaded with fluo‐3, fluo‐4 or fluo‐5F, to estimate dark, resting free Ca2+ concentration ([Ca2+]i) and changes in [Ca2+]i upon illumination. Dye concentration was adjusted to preserve the normal physiology of the rod, and the laser intensity was selected to minimise bleaching of the fluorescent dye. Wild‐type mouse rods illuminated continuously with laser light showed a progressive decrease in fluorescence well fitted by two exponentials with mean time constants of 154 and 540 ms. Rods from transducin α‐subunit knock‐out (Trα–/–) animals showed no light‐dependent decline in fluorescence but exhibited an initial rapid component of fluorescence increase which could be fitted with a single exponential (τ∼1–4 ms). This fluorescence increase was triggered by rhodopsin bleaching, since its amplitude was reduced by pre‐exposure to bright bleaching light and its time constant decreased with increasing laser intensity. The rapid component was however unaffected by incorporation of the calcium chelator BAPTA and seemed therefore not to reflect an actual increase in [Ca2+]i. A similar rapid increase in fluorescence was also seen in the rods of wild‐type mice just preceding the fall in fluorescence produced by the light‐dependent decrease in [Ca2+]i. Dissociation constants were measured in vitro for fluo‐3, fluo‐4 and fluo‐5F with and without 1 mm Mg2+ from 20 to 37 °C. All three dyes showed a strong temperature dependence, with the dissociation constant changing by a factor of 3–4 over this range. Values at 37 °C were used to estimate absolute levels of rod [Ca2+]i. All three dyes gave similar values for [Ca2+]i in wild‐type rods of 250 ± 20 nm in darkness and 23 ± 2 nm after exposure to saturating light. There was no significant difference in dark [Ca2+]i between wild‐type and Trα–/– animals.
Distributed cooperative adaptive identification for time-varying static parametric model systems with a cooperative PE condition by distributed event-triggered strategy

This paper addresses the problem of distributed cooperative adaptive identification for time-varying static parametric model systems with a new distributed event-triggered schedule. In the present framework, the cooperative persistent exciting (PE) condition is introduced to remove the existing assumption that the feasible solution of the controllers depended on the solving of line matrix inequality. By considering the communication resources constraints, a new event-triggered strategy is proposed to relax the requirement of continuous monitoring for each system. It is shown that, if the proposed assumpations are statisfied, the closed-loop adaptive systems are uniformly globally exponentially stable (UGES), the parameter estimation error converge to zero uniformly exponentially under a cooperative persistent excitation (PE) condition, and the Zeno behaviour is avoided by intoruding appropriate assumption that removes the state dependence requirement for each system.
GaAs MQW modulators integrated with silicon CMOS

We demonstrate integration of GaAs-AlGaAs multiple quantum well modulators to silicon CMOS circuitry via flip-chip solder-bonding followed by substrate removal. We obtain 95% device yield for 32/spl times/32 arrays of devices with 15 micron solder pads. We show operation of a simple circuit composed of a modulator and a CMOS transistor. <<ETX>>
Parametric drive of a toroidal MEMS rate integrating gyroscope demonstrating < 20 PPM scale factor stability

In this paper, we report parametric drive of a MEMS rate integrating gyroscope for reduction of drifts induced by drive electronics. A Toroidal Ring Gyroscope (TRG) with 100k Q-factor at 1760 μm diameter was fabricated in epitaxial silicon encapsulation (EpiSeal) process. Device was operated in rate integrating mode using a FPGA based control system. In contrast to the conventional amplitude control architecture, the central star electrode of the gyroscope, connected to a single drive channel was utilized for amplitude control of both modes. Due to the parametric pumping effect, energy added to each (x and y) mode is proportional to the existing amplitude of the respective mode. As a result, errors associated with finding the orientation of the standing wave and x-y drive gain drift are bypassed. Compared to conventional x-y drive architecture, as high as 14× improvement in scale factor stability was observed with parametric pumping, resulting in better than 20 ppm scale factor stability without any compensation or temperature stabilization.
Two step capture and purification of IgG2 using multicolumn countercurrent solvent gradient purification (MCSGP)

A two‐step chromatography process for monoclonal antibody (mAb) purification from clarified cell culture supernatant (cCCS) was developed using cation exchange Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) as a capture step. After an initial characterization of the cell culture supernatant the capture step was designed from a batch gradient elution chromatogram. A variety of chromatographic materials was screened for polishing of the MCSGP‐captured material in batch mode. Using multi‐modal anion exchange in bind‐elute mode, mAb was produced consistently within the purity specification. The benchmark was a state‐of‐the‐art 3‐step chromatographic process based on protein A, anion and cation exchange stationary phases. The performance of the developed 2‐step process was compared to this process in terms of purity, yield, productivity and buffer consumption. Finally, the potential of the MCSGP process was investigated by comparing its performance to that of a classical batch process that used the same stationary phase. Biotechnol. Bioeng. 2010;107: 974–984. © 2010 Wiley Periodicals, Inc.
Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma.

Abstract With slight modifications in the standard agar diffusion methods, it was possible to determine the concentrations of antibiotics in 0.08 ml. samples of serum or plasma. The mean error was ±11 per cent and the results were available after incubation for 112 to 312 hours at 37 °C. Gentamicin, kanamycin, streptomycin, neomycin, or vancomycin were rapidly assayed, even in the presence of penicillins or cephalosporins which could be readily inactivated with a zinc-requiring, "broad-spectrum" β-lactamase. The method was particularly helpful for regulation of the therapy in patients with diminished renal function in whom estimation of dosage on the basis of renal function was less reliable.
Spectral tilt modelling with extrapolated GMMs for intelligibility enhancement of narrowband telephone speech

Post-processing methods are used in mobile communications to improve the intelligibility of speech in adverse background noise conditions. In this study, post-processing based on the modification of the spectral tilt with Gaussian mixture models according to the Lombard effect is investigated. A spectral envelope estimation method is studied and optimized for this purpose. Furthermore, the extrapolation of the statistical mapping in a post-processing context is investigated. The proposed post-processing methods are compared to unprocessed speech and a reference method in subjective intelligibility and quality tests in different near-end noise conditions. The results indicate that one of the extrapolated methods achieved the same intelligibility as fixed high-pass filtering without degrading the quality of speech.