Miracle-dz/UniFAR-FTUs huggingface dataset:
Structural and functional characterization of a triple mutant form of S100A7 defective for Jab1 binding

S100A7 (psoriasin) is a calcium‐ and zinc‐binding protein implicated in breast cancer. We have shown previously that S100A7 enhances survival mechanisms in breast cells through an interaction with c‐jun activation domain binding protein 1 (Jab1), and an engineered S100A7 triple mutant (Asp56Gly, Leu78Met, and Gln88Lys—S100A73) ablates Jab1 binding. We extend these results to include defined breast cancer cell lines and demonstrate a disrupted S100A73/Jab1 phenotype is maintained. To establish the basis for the abrogated Jab1 binding, we have recombinantly produced S100A73, demonstrated that it retains the ability to form an exceptionally thermostable dimer, and solved the three dimensional crystal structure to 1.6 Å. Despite being positioned at the dimer interface, the Leu78Met mutation is easily accommodated and contributes to a methionine‐rich pocket formed by Met12, Met15, and Met34. In addition to altering the surface charge, the Gln88Lys mutation results in a nearby rotameric shift in Tyr85, leading to a substantially reorganized surface cavity and may influence zinc binding. The final mutation of Asp56 to Gly results in the largest structural perturbation shortening helix IV by one full turn. It is noteworthy that position 56 lies in one of two divergent clusters between S100A7 and the functionally distinct yet highly homologous S100A15. The structure of S100A73 provides a unique perspective from which to characterize the S100A7‐Jab1 interaction and better understand the distinct functions between S100A7, and it is closely related paralog S100A15.
Construction of a semi-automated ICD-10 coding help system to optimize medical and economic coding

INTRODUCTION In order to measure the medical activity in hospitals, physicians are required to code manually information concerning a patient's stay using ICD-10. This requires trained staff and a lot of time. We propose to help speed up and facilitate the tedious task of coding patient information. METHODS we show two methods. First, we propose an automated ICD-10-based coding help system using an automated MeSH-based indexing system and a mapping between MeSH and ICD-10 extracted from the UMLS metathesaurus. Secondly, we propose the use of drug prescriptions to complete the previous coding with the use of a mapping between a given prescription drug and the relevant ICD-10 codes (in compliance with the drug approval). RESULTS the results of a preliminary experiment indicate that the precision of the indexing system is 40% and the recall is 30% when we compare to an economic rules-based coding and to a descriptive coding. DISCUSSION moreover, we show that the use of prescription coding is relevant as the recall reaches 68% when the Vidal tool is used. CONCLUSION Then, it is very interesting to complete the coding obtained automatically by the indexing/mapping system by the coding obtained from the prescriptions.
Effect of Annealing Temperature on $\hbox{TiO}_{2}$ -Based Thin-Film-Transistor Performance

TiO<sub>x</sub> thin-film transistors (TFTs) are fabricated using SiO<sub>2</sub> as gate dielectrics. The enhancement of the electric characteristics is observed after a postannealing processing including the reduction of the threshold voltage <i>V</i><sub>th</sub>, the increase in mobility μ, and the on/off ratio. The effect of the postannealing temperature on both the TiO<sub>x</sub>/SiO<sub>2</sub> interfacial bonding structure and the TiO<sub>x</sub> crystallinity is investigated. We suggest that the interfacial modification at the TiO<sub>x</sub>/SiO<sub>2</sub> interface contributes to the significant reduction of <i>V</i><sub>th</sub> due to the breaking of Si-O-Ti bonding. The improvement of the TiO<sub>x</sub> crystallinity and interfacial structure leads to the increase in μ and in the on/off ratio. The low-temperature annealing treatment at 200 <sup>°</sup>C is very effective to improve the TiO<sub>x</sub>/SiO<sub>2</sub> interface structure.
Duration of Sexual Harassment and Generalized Harassment in the Workplace Over Ten Years: Effects on Deleterious Drinking Outcomes

ABSTRACT Although harassment in the workplace has been linked to deleterious drinking outcomes, researchers have yet to examine the long-term effects of chronic workplace harassment. During a 10-year longitudinal mail survey, university employees (N = 2,265) were administered measures of sexual harassment, generalized workplace harassment, and problematic drinking. Using growth mixture modeling, two latent classes of workplace harassment emerged: infrequent and chronic. Demographic characteristics (gender, age, and race) predicted the shape of the trajectories and likelihood of class membership. As hypothesized, membership in the chronic harassment classes was linked to future problematic drinking, even after controlling for previous drinking.
Discontinuation of Systematic Surveillance and Contact Precautions for Vancomycin-Resistant Enterococcus (VRE) and Its Impact on the Incidence of VRE faecium Bacteremia in Patients with Hematologic Malignancies

OBJECTIVE To study the effect of discontinuation of systematic surveillance for vancomycin-resistant Enterococcus (VRE) and contact isolation of colonized patients on the incidence of VRE bacteremia SETTING A hematology-oncology unit with high prevalence of VRE colonization characterized by predominantly sporadic molecular epidemiology PARTICIPANTS Inpatients with hematologic malignancies and recipients of hematopoietic stem cell transplantation METHODS The incidence of VRE bacteremia was measured prospectively during 2 different 3-year time periods; the first during active VRE surveillance and contact precautions and the second after discontinuation of these policies. We assessed the collateral impact of this policy change on the incidence of bacteremia due to methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile infection even though we maintained contact precautions for these organisms. Incidence of infectious events was measured as number of events per 1,000 patients days per month. Time series analysis was used to evaluate trends. RESULTS The incidence of VRE bacteremia remained stable after discontinuation of VRE surveillance and contact precautions. The incidence of MRSA bacteremia and Clostridium difficile infection for which we continued contact precautions also remained stable. Aggregated antibiotic utilization and nursing hours per patient days were similar between the 2 study periods. CONCLUSION Active surveillance and contact precautions for VRE colonization did not appear to prevent VRE bacteremia in patients with hematologic malignancies and recipients of hematopoietic stem cell transplantation with high prevalence of VRE characterized by predominantly sporadic molecular epidemiology. Infect. Control Hosp. Epidemiol. 2016;37(4):398–403
Experimental and Theoretical Analyses of Axial Magnetic Coupling Under Steady-State and Transient Operations

This paper investigates the steady-state and transient performances of an axial magnetic coupling by using analytical formulas issued from an analytical model based on a 2-D approximation for the magnetic field distribution (mean radius model). From the magnetic field expression, simple analytical formulas are derived for computing the pull-out torque and the torsional stiffness of the coupling as a function of the geometrical parameters. Here, a special attention is given to the overload torque condition during the transient which leads to the loss of synchronism for the coupling. Moreover, radial and angular misalignment conditions are also studied. In order to study the accuracy of the proposed analytical model, the results are compared with those obtained from 3-D finite-element simulations and measurements.
Probability density function for frequency response function measurements using periodic signals

Frequency response function (FRF) measurements are often used to characterize linear dynamic systems. Nowadays uncertainty bounds on FRF measurements still are based on linear approximations, which are valid for sufficiently large input signal-to-noise-ratios (SNR) only. In this paper exact uncertainty bounds are calculated, which are valid for any input SNR. These bounds are obtained via an analytic expression of the probability density function (PDF) of the FRF measurements. The results are valid for open- and closed-loop measurements, and the theory is illustrated on a real measurement example.
Rho GTPase Is Activated by Cytotoxic Necrotizing Factor 1 in Peripheral Blood T Lymphocytes: Potential Cytotoxicity for Intestinal Epithelial Cells

ABSTRACT Some strains of Escherichia coli related to acute cystitis or colitis produce a toxin named cytotoxic necrotizing factor 1 (CNF-1). CNF-1 mediates its effects on epithelial cells or phagocytes via the permanent activation of small GTP-binding proteins, caused by the toxin-induced deamidation of Glu63 of p21 Rho. The behavior of peripheral blood T lymphocytes during the acute phase of bacterial colitis has been poorly investigated. Our study was conducted to test whether (i) peripheral blood T lymphocytes can be activated by CNF-1 and (ii) CNF-1-activated T lymphocytes are cytotoxic against intestinal epithelial cells. Activation of T lymphocytes by CNF-1 was assessed by electrophoresis, flow cytometry, confocal microscopy, and electron microscopy studies. Assays for migration and adherence of CNF-1-treated T lymphocytes were performed in Transwell chambers with T84 intestinal epithelial cells grown on polycarbonate semipermeable filters. CNF-1 induced a decrease in the electrophoretic mobility of the GTP-binding protein Rho in treated T lymphocytes. CNF-1 provoked an increase in the content of actin stress fibers and pseudopodia in T lymphocytes. Several adherence molecules were clustered into cytoplasmic projections in CNF-1-treated T lymphocytes and adherence of such lymphocytes on the basolateral pole of T84 was increased, resulting in cytotoxicity toward epithelial cells. Such enhanced adherence in response to CNF-1 was dependent on p42-44MAP kinase activation of T lymphocytes. Taken together, these results suggest that CNF-1, by acting on T lymphocytes, may increase in an important fashion the virulence of certain strains of E. coli against the intestinal epithelia.
Preservation of mouse liver tissue during cold storage in experimental solutions assessed by x‐ray microanalysis

The increasing use of organs for transplantation necessitates the development of optimal preservation techniques. The goal of this study was to investigate changes in elemental content in mouse liver cells during cold storage by x‐ray microanalysis in parallel with morphologic studies. Tissue was stored at 4°C for 4 to 12 hours in normal Krebs‐Ringer solution (high sodium/potassium ratio), modified Krebs‐Ringer solution (low Na+/K+ ratio), Euro‐Collins solution, University of Wisconsin (UW) solution, or seven modified versions of the UW solution. Incubation of liver in normal Krebs‐Ringer solution caused a significant increase in sodium and decrease in potassium concentrations in contrast to incubation in other solutions. The concentration of sodium, potassium, and chlorine in the cells closely followed the concentration in the storage solution, indicating that the intracellular concentration of these ions during storage is entirely dependent on diffusion processes. The calcium concentration was independent of the storage solution used. Studies by light and transmission electron microscopy showed good preservation of hepatocytes after storage for 8 and 12 hours in UW solution and its variants, modified Krebs‐Ringer solution and Euro‐Collins solution, but showed moderate damage to mitochondria and swelling of the endoplasmic reticulum in normal Krebs‐Ringer solution. In addition, damage to the sinusoidal endothelial cells was observed after 4 hours in normal Krebs‐Ringer solution and after 8 to 12 hours in the other solutions. In conclusion, the only factor determining the intracellular concentration of diffusible ions after cold tissue storage is the ionic composition of the extracellular medium. X‐ray microanalysis provides an objective method for assessing whether the intracellular ionic composition of tissue is maintained during storage.
Daily hassles and social support as predictors of adjustment in children with pediatric rheumatic disease.

OBJECTIVE To test hypotheses that social support moderates the effects of microstressors on the psychosocial adjustment of children with pediatric rheumatic diseases (PRDs) and that among multiple sources of support, classmate and parent support are significant predictors of adjustment, after controlling for demographic and disease severity variables. METHODS Children with PRDs (N = 160 children; 8-17 years) were recruited from three pediatric rheumatology centers and completed measures of daily hassles, social support, depressive symptoms, and state and trait anxiety; their parents completed measures of internalizing and externalizing behaviors. RESULTS Fewer daily hassles and higher social support predicted fewer adjustment problems. Among the sources of support, classmate and parent support were significant predictors. Tests for moderation were significant only for a Hassles x Classmate Support interaction in the prediction of depression. A plot of the interaction between hassles and classmate support showed that children with high classmate support had lower levels of depression than children with low classmate support under high or low levels of daily hassles. Furthermore, children with high classmate support had lower levels of depression under conditions of low versus high daily hassles. DISCUSSION Results are consistent with a main effect rather than buffering model for social support. CONCLUSIONS Interventions should focus on management of daily hassles and increasing social support for children with PRDs.
The Biocide Chlorine Dioxide Stimulates Biofilm Formation in Bacillus subtilis by Activation of the Histidine Kinase KinC

ABSTRACT Bacillus subtilis forms biofilms in response to signals that remain poorly defined. We report that biofilm formation is stimulated by sublethal doses of chlorine dioxide (ClO2), an extremely effective and fast-acting biocide. ClO2 accelerated biofilm formation in B. subtilis as well as in other bacteria, suggesting that biofilm formation is a widely conserved response to sublethal doses of the agent. Biofilm formation depends on the synthesis of an extracellular matrix that holds the constituent cells together. We show that the transcription of the major operons responsible for the matrix production in B. subtilis, epsA-epsO and yqxM-sipW-tasA, was enhanced by ClO2, in a manner that depended on the membrane-bound kinase KinC. Activation of KinC appeared to be due to the ability of ClO2 to collapse the membrane potential. Importantly, strains unable to make a matrix were hypersensitive to ClO2, indicating that biofilm formation is a defensive response that helps protect cells from the toxic effects of the biocide.
Hierarchical multiple associative mapping in cache memories

In this paper, a new cache placement scheme is proposed to achieve higher hit ratios with respect to the two conventional schemes namely set-associative and direct mapping. Similar to set-associative, in this scheme, cache space is divided into sets of different sizes. Hence, the length of tag fields associated to each set is also variable and depends on the partition it is in. The proposed mapping function has been simulated with some standard trace files and statistics are gathered and analyzed for different cache configurations. The results reveal that the proposed scheme exhibits a higher hit ratio compared to the two well-known mapping schemes, namely set-associative and direct mapping, using LRU replacement policy. Also, its area and power consumption is less than full-associative scheme.
Consumer Information Privacy and the Problem(s) of Third-Party Disclosures

U.S. consumers have little actual control over how companies collect, use, and disclose their personal information. This Article identifies two specific instances of this lack of control under U.S. law related to third-party disclosures, what I call the Incognito and Onward Transfer Problems. It then identifies the types of privacy harms that result and examines the advantages and possible drawbacks of a model law aimed at addressing these specific problems. The model law is based on a system of consumer notice and choice, the predominant method used in the U.S. to provide consumers with control over their information. Up until this point, however, this method of providing control has largely failed, and this Article seeks to address some of its failures. This Article argues that while notice and choice may be useful in addressing some information privacy problems (such as the two identified in this Article), it is not appropriate for all information privacy problems. No one-size-fits-all approach is adequate. Instead, each information privacy problem must be isolated and treated in its proper context.
A factor-searching-based multiple string matching algorithm for intrusion detection

Multiple string matching plays a fundamental role in network intrusion detection systems. Automata-based multiple string matching algorithms like AC, SBDM and SBOM are widely used in practice, but the huge memory usage of automata prevents them from being applied to a large-scale pattern set. Meanwhile, poor cache locality of huge automata degrades the matching speed of algorithms. Here we propose a space-efficient multiple string matching algorithm BVM, which makes use of bit-vector and succinct hash table to replace the automata used in factor-searching-based algorithms. Space complexity of the proposed algorithm is O(rm2 + ΣpϵP |p|), that is more space-efficient than the classic automata-based algorithms. Experiments on datasets including Snort, ClamAV, URL blacklist and synthetic rules show that the proposed algorithm significantly reduces memory usage and still runs at a fast matching speed. Above all, BVM costs less than 0.75% of the memory usage of AC, and is capable of matching millions of patterns efficiently.
Discussion of 'Bayesian Nonparametric Inference - Why and How', by Peter Müller and Riten Mitra.

Muller and Mitra’s contribution regarding the practical whys and hows of non-parametric Bayes (NPB) is welcome. In that spirit, we highlight one basic and one complex social science example for which NPB is uniquely well-suited. Depression symptoms scores were collected from n = 299 clients on three occasions – pre-treatment, post-treatment, and follow-up – during a study of group therapy’s effectiveness for treating depression. Clients completed up to four group therapy modules and could join the therapy group at start of a module. Therapy group-induced correlations among client outcomes could thus be modeled using random module effects, which would be linked to post-treatment outcomes via multiple membership, and client-specific growth parameters (e.g., random intercept, time, and quadratic time effects) could be specified for modeling within-client correlations and deviations from the average depression score trajectory (Paddock and Savitsky 2013).
Mini Nutritional Assessment

Purpose of reviewTo summarize recent evidences and advances on the implementation and the use of the Mini Nutritional Assessment (MNA). Recent findingsDespite being introduced and validated for clinical use about 20 years ago, the MNA has recently received new attention in order to more widely disseminate among healthcare professionals the practice of a systematic nutritional screening and assessment of the old patient. Particularly, the structure has been implemented to face the difficulties in having the patients contributing to the assessment and to reduce further the time required to complete the evaluation. Recent data also confirm that in older populations prevalence of malnutrition by this tool is associated with the level of dependence. The rationale of nutritional assessment is to identify patients candidate to nutritional support. However, the sensitivity of the MNA is still debated because it has been associated with a high-risk ‘overdiagnosis’ and the advantages of a positive screening need to be assessed both in terms of outcome and money saving. SummaryThe MNA is a simple and highly sensitive tool for nutritional screening and assessment. The large mass of data collected and the diffusion among healthcare professionals clearly support its use. However, the cost-effectiveness of interventions based on its scoring deserves investigation.
Age, Wage and Productivity

Previous empirical studies on the effect of age on productivity and wages find contradicting results. Some studies find that if workers grow older there is an increasing gap between productivity and wages, i.e. wages increase with age while productivity does not or does not increase at the same pace. However, other studies find no evidence of such an age related pay-productivity gap. We perform an analysis of the relationship between age, wage and productivity using a matched worker-firm panel dataset from Dutch manufacturing covering the period 2000-2005. We find little evidence of an age related pay-productivity gap.
The pupil location based on the Laplace operator and region-growing

In this paper we present a pupil location method based on the Laplace operator and region-growing. Firstly, it finds a point in the pupil by gray summing operator for ensuring the beginning point of region-growing in the pupil, then it adopts Laplace operator to sharpen the image and reduce the interference of eyelash and eyelid for region-growing. Thirdly it ensures the pupil area by region-growing method and gets binary image. Finally in the binary image it uses Hough transform in certain area to fit the pupil edge. 756 eye images comprising an eye image database are located, our algorithm reaches 100% at accuracy. What is more, it ensures the robustness of location.
A framework-based approach to build adaptive multimedia applications with quality of service

The current Internet provides a single class best-effort service. However, the continuous data traffic (e.g., audio and video) of new applications that are emerging over the Internet have different characteristics if compared to conventional data traffic (e.g., text and images) and consequently have different QoS (quality of service) requirements. Several approaches have been developed trying to guarantee QoS. The development of applications that have the capability to adapt their behavior, depending on the network performance, is one example of such approaches. Inside this context, this paper introduces the development of application programming interface (API) to incorporate QoS in multimedia applications. This API has QoS monitoring functions through RTCP (real time control protocol) packets and QoS adaptation functions through the application of adaptation policies.