Miracle-dz/UniFAR-FTUs huggingface dataset:
Critical roles for excretory–secretory cysteine proteases during tissue invasion of Paragonimus westermani newly excysted metacercariae

Paragonimus westermani is a trematode parasite, which causes pulmonary and/or extrapulmonary granulomatous disease in humans. Successful invasion of the host tissue is critical for the survival of this tissue‐invasive parasite. The enzymatic hydrolysis of host proteins is clearly a prerequisite of this process. In this study, we have investigated the functional roles of the excretory–secretory cysteine proteases of P. westermani newly excysted metacercariae (PwNEM) in tissue invasion. The 27 and 28 kDa enzymes (PwMc27 and PwMc28) purified from PwNEM excretory–secretory products (ESP), preferentially degraded fibrillar proteins, but not globular proteins. PwMc28 significantly facilitated the invasion of PwNEM into mouse peritoneum, whereas a diffusible cysteine protease inhibitor, trans‐epoxysuccinyl‐l‐leuciloamido‐(4‐guanidino) butane (E‐64) inhibited this process dose‐dependently. Two distinct isoforms of PwMc28 (PwMc28a and PwMc28b), which exhibited two amino acid differences in their mature domains, were identified by tandem mass spectrometry and sequence analysis. Both enzymes were localized at the tegument on the anterior border and on the oral sucker, which suggests excretion–secretion via exocytosis or via the excretory canal network. The mRNA transcripts of PwMc28a and b were expressed abundantly during the active invasion/migration through the host’s tissues, suggesting their relevant function to tissue invasion/migration in the definitive host.
Ultrasonic beam focusing through tissue inhomogeneities with a time reversal mirror: application to transskull therapy

Time reversal of ultrasonic fields allows a very efficient approach to focus pulsed ultrasonic waves through lossless inhomogeneous media. Time reversal mirrors (TRMs) are made of large transducer arrays, allowing the incident field to be sampled, time reversed, and reemitted. Time reversal method corrects for phase, amplitude, and even shape aberration and thus, is more efficient than time shift compensation techniques. However, this technique needs the knowledge of the Green's function of a dominant scatterer available in the medium. Aberration correction for ultrasonic hyperthermia could be achieved by combining TRM with an artificial acoustic source or sensor implanted inside the treatment volume. In this paper, time reversal method has been experimentally applied to the focusing through the skull bone. It is shown that the skull induces severe attenuation of ultrasound and reduces the efficiency of the time reversal approach. Then, an amplitude correction method is proposed to focus through an attenuating layer located close to the array. This method consists in inversing the amplitude modulation and then time reversing these signals. Finally, this method is combined with numerical backpropagation to compensate for an attenuating layer located some distance away from the transducer array.
Service use by Australian children for emotional and behavioural problems: Findings from the second Australian Child and Adolescent Survey of Mental Health and Wellbeing

Objective: To identify the proportion of children and adolescents in Australia and the proportion of those with mental disorders who used services for emotional and behavioural problems, the type of services used and what characteristics were associated with service use. Method: During 2013–2014, a national face-to-face household survey of mental health and wellbeing (Young Minds Matter) was conducted, involving 6310 parents and carers of 4- to 17-year-olds (55% of eligible households) and self-report surveys from 2967 11- to 17-year-olds in these households (89% of eligible youth). The survey identified 12-month mental disorders based on the Diagnostic Interview Schedule for Children–Version IV and asked about service use for emotional or behavioural problems in the previous 12 months. Results: Overall, 17.0% of all 4- to 17-year-olds used services for emotional or behavioural problems in the previous 12 months. Of those with mental disorders, 56.0% used services (48.9% of 4- to 11-year-olds; 65.1% of 12- to 17-year-olds). Service use was highest among 4- to 17-year-olds with major depressive disorder (79.6%) and lowest for those with attention-deficit/hyperactivity disorder (52.7%). Two-fifths (41.2%), 72.5% and 87.6% of those with mild, moderate and severe disorders used services. General practitioners, psychologists, paediatricians and counsellors/family therapists were the most commonly accessed health service providers. Two-fifths with mental disorders had attended school services. About 5% of adolescents reported use of online personal support or counselling for help with their problems. From multivariate models, service use was higher in sole carer families, but also among those living in the least socially and economically disadvantaged compared to the most disadvantaged areas. Conclusion: Rates of service use for mental disorders in Australia’s children and adolescents appear to have increased substantially. Health services and schools are the major providers of services for emotional and behavioural problems, but telephone counselling and online services have become well-established parts of the service environment.
X-linked retinitis pigmentosa: mutation spectrum of the RPGR and RP2 genes and correlation with visual function.

PURPOSE To assess the frequency of RPGR and RP2 mutations in a set of 85 patients with X-linked retinitis pigmentosa (XLRP) and to compare the visual function of patients with mutations in RPGR versus RP2. METHODS Eighty-five unrelated patients with XLRP were ascertained, mainly from North America. The single-strand conformation polymorphism (SSCP) and a direct sequencing technique were used to screen their DNA for mutations in the coding region and splice sites of RPGR and RP2. The Snellen visual acuities, visual field areas, and 0.5-Hz and 30-Hz electroretinograms (ERGs) were measured in male patients. The visual function parameters were compared using multiple regression analysis. RESULTS A wide spectrum of mutations was found in both genes, including missense, nonsense, splice-site, and frameshift mutations. Twenty putative pathogenic mutations in RPGR, 15 of which were novel, were found in 22 patients (26%), whereas 6 mutations in RP2, 4 of which were novel, were found in 6 patients (7%). A high fraction of the mutations in both genes affected amino acid residues within or adjacent to presumed functional domains. Comparison of visual function between comparably aged patients with mutations in RPGR versus RP2 showed that, on average, patients with RPGR mutations have lower ERG amplitudes and smaller visual field areas. CONCLUSIONS Mutations in RPGR and RP2 genes together account for approximately 33% of cases of XLRP in North America. Patients with RPGR mutations have less overall retinal function on average than those with RP2 mutations, on the basis of measurements of visual field areas and full-field ERG amplitudes.
Material Selection of Natural Fibers for Composite Automotive Component Using Analytic Hierarchy Process/Analytic Network Process in Concurrent Engineering Approach

Material selection of natural fiber for composite based products is an essential process in product design development especially in application of automotive components. Several factors need to be considered concurrently and dependency among the factors should be evaluated during the judgement. Therefore, a proposed approach is presented in this study by using Analytic Hierarchy Process/Analytic Network Process (AHP/ANP) to prioritize the criteria for material selection of natural fiber composites automotive components. AHP computes the priority values from pairwise comparison basis and ANP derives the global priority values based on the inner dependency of the criteria. As in a case study, flax fibers obtained the highest value (19%) and rank as number one fibers in the selection where tensile strength is the most significant criteria. Thus, application of AHP/ANP would derive a more reliable and accurate judgement in material selection and their flexibility allows integration with other decision making tools.
Glucocorticoids in muscular dystrophy: beneficial effects of dexamethasone on avian myopathy

A corticosteroid with mixed glucocorticoid‐mineralo‐corticoid actions was previously shown to improve neuromuscular function in muscular dystrophic chickens. The significance of that finding was recently underscored by reports that a mixed‐action corticosteroid improved muscle function in Duchenne dystrophy patients, albeit at high doses. In the present study a pure glucocorticoid improved function and retarded muscle histopathology in the chicken, but a pure mineralocorticoid did not. These observations suggest that elucidation of mechanisms by which glucocorticoids beneficially affect dystrophic muscle could lead to development of more effective therapies. — Entrikin, R. K. ; Abresch, R. T; Bradford, D. P.; Larson, D. B. ; Longley, K.J.; Wilson, B. W. Glucocorticoids in muscular dystrophy: beneficial effects of dexamethasone on avian myopathy. FASEB J. 2: 2722‐2725; 1988.
Online Stigma Resistance in the Pro-Ana Community

Media scholars often use concepts from Goffman’s dramaturgical approach to study online communities of stigmatized individuals as “backstages,” spaces where members take refuge from social disapproval. In this study, I extend this view through an examination of in-depth interviews with bloggers from the “pro-ana” community, an online community for people with eating disorders. To explore how this community uses an online environment that is both anonymous and public, I fuse Goffman’s ideas about identity performance and stigma with more recent theories about boundary maintenance. In-depth interviews with “pro-ana” bloggers reveal that to protect this virtual group and resist stigmas associated both with their illness and with their online presence, they construct their own norms and rules in the online realm, and discipline and eject members deemed to be out-group.
Continuous software engineering and its support by usage and decision knowledge: An interview study with practitioners

Continuous software engineering (CSE) emerged as a process that is increasingly applied by practitioners. However, different perceptions impede its adoption in industry. Furthermore, opportunities through utilizing usage and decision knowledge remain unexploited. We conducted a semi‐structured interview study with 24 practitioners from 17 companies to study how practitioners apply CSE during software evolution and how usage and decision knowledge can support CSE. Regarding the application of CSE, we identified five perspectives on CSE with tool‐ and methodology‐driven definitions most prevalent. Automated tests, involved users, and shared rulesets are perceived as most relevant for CSE. Practitioners report more positive than negative experiences; however, more than half of their responses were neutral. Practitioners' future plans for CSE focus on enhancement, expansion, and on‐demand adaption. Regarding the integration of usage and decision knowledge into CSE, practitioners perceive accountability and traceability as major benefits, while raising concerns about its feasibility and user groups. As short‐term extensions, practitioners expect improvements regarding automation and role aspects, while long‐term additions to integration and experimentation capabilities are demanded. We conclude that CSE remains partially difficult to capture for practitioners, while their attitude toward integrating usage and decision knowledge into CSE is positive.
A Hybrid Mitigation Technique for Malicious Network Traffic based on Active Response

The rapid increase in advanced persistent threats in the cyber space engenders full attention to the use of intrusion detection with emphasis on Artificial Intelligence-based intrusion detection systems as a mitigation mechanism. The sharp increase in attack surfaces can be partially attributed to the fact that Internet becomes the de facto means of converged communications and online transactions accommodating different types of services under the same scheme. Most current intrusion detection systems (IDS) deploy signature patterns of known attacks and anomaly detection approaches in detecting intrusions in an attempt to reduce the computational complexity introduced by large scale data sets. However, these approaches have been proved to be inadequate to detect novel attacks often resulting in a high false positive rate. This research will therefore seek to address the issue of detecting persistent network threats by combining the approaches of misuse and anomaly detection in one system. Our algorithm incorporates the concept of active response against all four broad attack types analyzed in the literature to realize another algorithm for intrusion detection and prevention as well as active response called HYBRITQ-4. The algorithm introduces a mechanism for classifying packets based on protocol information to enhance pattern searches and matching when detecting abnormal packets. Findings from our investigation suggest that the proposed algorithm can efficiently improve the detection rate, false positive rate and accuracy of detecting intrusions in patterns of known and novel attacks.
Functional hearing in the classroom: assistive listening devices for students with hearing impairment in a mainstream school setting

Abstract Objective: To assess the benefit of assistive listening devices (ALDs) for students with hearing impairment in mainstream schools. Design: Speech recognition (CNC words) in background noise was assessed in a typical classroom. Participants underwent testing using four device configurations: (1) HA(s)/CI(s) alone, (2) soundfield amplification, (3) remote microphone (Roger Pen) on desk and (4) remote microphone at the loudspeaker. A sub-group of students subsequently underwent a 2-week classroom trial of each ALD. Degree of improvement from baseline [HA(s)/CI(s)] alone was assessed using teacher and student Listening Inventory for Education-Revised (LIFE-R) questionnaires. Study sample: In all, 20 students, aged 12.5–18.9 years, underwent speech recognition assessment. In total, 10 of these participated in the classroom trial. Hearing loss ranged from mild-to-profound levels. Results: Performance in each ALD configuration was higher than for HAs/CIs alone (p < 0.001). Teacher and student LIFE-R results indicated significant improvement in listening/communication when using the remote microphone in conjunction with HAs/CIs (p < 0.05). There was no difference between the soundfield system and the baseline measurement (p > 0.05). Conclusion: Speech recognition improvements were demonstrated with the implementation of both remote microphones and soundfield systems. Both students and teachers reported functional hearing advantages in the classroom when using the remote microphone in concert with their standard hearing devices.
Using the Theory of Planned Behavior to Examine Beliefs About Verbal Sexual Coercion Among Urban Black Adolescents

This study used the theory of planned behavior to identify urban Black adolescents’ beliefs about male-to-female verbal sexual coercion. Interviews were conducted with 91 urban, Black adolescents (53 boys and 38 girls) to identify their behavioral, normative, and control beliefs about verbal sexual coercion perpetration (for males) and resistance (for females). Boys reported that perpetrating verbal sexual coercion could result in negative relationship outcomes, and the main benefit of using this tactic was to obtain sex. Unsupportive peers and some male family members were seen as encouraging boys to use verbal sexual coercion, whereas parents were seen as opposed to the use of coercion. Being in a private context with a girl and having persuasive skill were seen as facilitating the use of coercion, whereas being with an experienced or skilled girl was a barrier. For girls, positive relationship outcomes and sexual health risks were the benefits and pitfalls of resisting verbal sexual coercion. “Real” friends and family were described as supporting girls’ resistance to coercion, while “fake” friends, promiscuous girls, and male peers were seen as not supporting resistance. Girls believed being in a safe, public context would make it easier to resist coercion, while being in a stable relationship with an attractive and persistent partner would make it more difficult to resist. Knowing urban, Black adolescents’ beliefs about verbal sexual coercion is the first step toward predicting and intervening on their perpetration and resistance behaviors.
Tumor p38MAPK signaling enhances breast carcinoma vascularization and growth by promoting expression and deposition of pro-tumorigenic factors

The breast carcinoma microenvironment strikingly influences cancer progression and response to therapy. Various cell types in the carcinoma microenvironment show significant activity of p38 mitogen-activated protein kinase (MAPK), although the role of p38MAPK in breast cancer progression is still poorly understood. The present study examined the contribution of tumor p38MAPK to breast carcinoma microenvironment and metastatic capacity. Inactivation of p38MAPK signaling in metastatic breast carcinoma cells was achieved by forced expression of the kinase-inactive mutant of p38/MAPK14 (a dominant-negative p38, dn-p38). Disruption of tumor p38MAPK signaling reduced growth and metastases of breast carcinoma xenografts. Importantly, dn-p38 markedly decreased tumor blood-vessel density and lumen sizes. Mechanistic studies revealed that p38 controls expression of pro-angiogenic extracellular factors such as matrix protein Fibronectin and cytokines VEGFA, IL8, and HBEGF. Tumor-associated fibroblasts enhanced tumor growth and vasculature as well as increased expression of the pro-angiogenic factors. These effects were blunted by dn-p38. Metadata analysis showed elevated expression of p38 target genes in breast cancers and this was an unfavorable marker of disease recurrence and poor-outcome. Thus, our study demonstrates that tumor p38MAPK signaling promotes breast carcinoma growth, invasive and metastatic capacities. Importantly, p38 enhances carcinoma vascularization by facilitating expression and deposition of pro-angiogenic factors. These results argue that p38MAPK is a valuable target for anticancer therapy affecting tumor vasculature. Anti-p38 drugs may provide new therapeutic strategies against breast cancer, including metastatic disease.
Structure, dynamics, and evolution of centromeric nucleosomes

Centromeres are defining features of eukaryotic chromosomes, providing sites of attachment for segregation during mitosis and meiosis. The fundamental unit of centromere structure is the centromeric nucleosome, which differs from the conventional nucleosome by the presence of a centromere-specific histone variant (CenH3) in place of canonical H3. We have shown that the CenH3 nucleosome core found in interphase Drosophila cells is a heterotypic tetramer, a “hemisome” consisting of one molecule each of CenH3, H4, H2A, and H2B, rather than the octamer of canonical histones that is found in bulk nucleosomes. The surprising discovery of hemisomes at centromeres calls for a reevaluation of evidence that has long been interpreted in terms of a more conventional nucleosome. We describe how the hemisome structure of centromeric nucleosomes can account for enigmatic properties of centromeres, including kinetochore accessibility, epigenetic inheritance, rapid turnover of misincorporated CenH3, and transcriptional quiescence of pericentric heterochromatin. Structural differences mediated by loop 1 are proposed to account for the formation of stable tetramers containing CenH3 rather than stable octamers containing H3. Asymmetric CenH3 hemisomes might interrupt the global condensation of octameric H3 arrays and present an asymmetric surface for kinetochore formation. We suggest that this simple mechanism for differentiation between centromeric and packaging nucleosomes evolved from an archaea-like ancestor at the dawn of eukaryotic evolution.
Introducing the objective structured clinical examination to a general practice residency programme: results of a French pilot study

OSCE for evaluating clinical competence still remains limited in France. This study presents the results of the first experimental use of an OSCE as a formative assessment of French general practice trainees. Fifty trainees rotated through a circuit of 15 standardized patient-based OSCE cases. Differences in scores were determined by analysis of variance. Reliability was calculated with the coefficient alpha. Pearson correlations were used to determine the relationship between station scores and overall OSCE score. Written questionnaires based on Likert-type scales were used to measure OSCE feasibility. No difference was found between total session scores. Significant item-total score correlations were found for 12 of the 15 clinical problems. The reliability of the examination was 0.58. Most participants agreed that the clinical situations were realistic, simulated patients were believable and sampling of cases was representative of general practice. These data confirm the feasibility of OSCE in assessing performance of general practice trainees. Optimal training of observers should improve examination reliability. This study warrants further development to confirm its usefulness as a summative assessment tool.
Two essays on monetary policy under the Taylor rule

Two Essays on Monetary Policy Under the Taylor Rule. (August 2004) Jeong Eui Suh, B.S., Korea University Co–Chairs of Advisory Committee: Dr. Dror Goldberg Dr. Qi Li In this dissertation, two questions concerning monetary policy under the Taylor rule have been addressed. The first question is on, under the Taylor rule, whether a central bank should be responsible for both bank supervision and monetary policy or whether the two tasks should be exercised by separate institutions. This is the main focus of Chapter I. The second question is on whether the Taylor rule plays an important role in explaining modern business cycles in the United States. The second question has been covered by Chapter II. The implications of the first chapter can be summarized as follows: (i) it is inevitable for the central bank to have a systematic error in conducting monetary policy when the central bank does not have a bank supervisory role; (ii) without a bank supervisory role, the effectiveness of monetary policy cannot be guaranteed; (iii) because of the existence of conflict of interests, giving a bank supervisory role to the central bank does not guarantee the effectiveness of monetary policy, either; (iv) the way of setting up another government agency, bank regulator, and making the central bank and the regulator cooperate each other does not guarantee the effectiveness of monetary policy because, in this way, the systematic error in conducting monetary policy cannot be eliminated; (v) in the view of social welfare, not in the view of the effectiveness of monetary policy, it is better for the central bank to keep the whole