Miracle-dz/UniFAR-FTUs huggingface dataset:
Is Lgi2 the Candidate Gene for Partial Epilepsy with Pericentral Spikes?

Partial epilepsy with pericentral spikes (PEPS) is a familial epilepsy with disease locus mapped to human chromosome region 4p15; yet, the causative gene is unknown. In this work, arguments based on protein sequence analysis and patient-specific chromosomal deletions are provided for LGI2 as the prime candidate gene for PEPS among the 52 genes known at the genome locus 4p15. Furthermore, we suggest that two reports of patients that were not classified as PEPS but show very similar phenotypes and deletions in the PEPS disease locus, could in fact describe the same disease. To test this hypothesis, patients with diagnosed PEPS or the described similar phenotypes could be screened for mutations in LGI2 and other shortlisted candidate genes. The linkage between PEPS and its disease causing gene(s) would allow diagnosis of the disease based on genetic screening as well as hereditary studies. Furthermore, previous knowledge on molecular disease mechanisms of related LGI proteins, for example LGI1 and autosomal dominant lateral temporal epilepsy, could be applied to deepen the understanding of the PEPS disease mechanism at the molecular level, which may facilitate therapeutic intervention in the future. Supplementary Table is available at http://www.worldscinet. com/jbcb/.
Xanthan Gum Removal for 1H-NMR Analysis of the Intracellular Metabolome of the Bacteria Xanthomonas axonopodis pv. citri 306

Xanthomonas is a genus of phytopathogenic bacteria, which produces a slimy, polysaccharide matrix known as xanthan gum, which involves, protects and helps the bacteria during host colonization. Although broadly used as a stabilizer and thickener in the cosmetic and food industries, xanthan gum can be a troubling artifact in molecular investigations due to its rheological properties. In particular, a cross-reaction between reference compounds and the xanthan gum could compromise metabolic quantification by NMR spectroscopy. Aiming at an efficient gum extraction protocol, for a 1H-NMR-based metabolic profiling study of Xanthomonas, we tested four different interventions on the broadly used methanol-chloroform extraction protocol for the intracellular metabolic contents observation. Lower limits for bacterial pellet volumes for extraction were also probed, and a strategy is illustrated with an initial analysis of X. citri’s metabolism by 1H-NMR spectroscopy.
Role of T Cell‐Derived Lymphokines in Two Models of B‐Cell Tolerance

Engagement of surface immutioglobulin (slg) receptors by antigen initiates a series of biochemical signals that affect B-cell growth and differentiation. When this interaction occurs with immature (e.g. neonatal) B cells, a state of unresponsiveness or tolerance usually results. In contrast, mature B cells can be activated (i.e. positively signalled to proliferate/difTerentiate) by crosslinking of their slg receptors by antigen or anti-Ig antibodies. However, mature B cells also can be rendered unresponsive by concomitant interaction of anti-Ig/tolerogen with Fc receptors (Klaus et al. 1987, Waldschmidt & Vitetta 1985, Warner and Scott, unpublished). Several model systems employing polyclonal populations of normal B cells have been used to examine the molecular pathways of positive signalling via slg (Moller 1987). Over the past decade, our laboratory has been examining the biochemical events associated with both positive and negative signalling in relatively homogeneous populations of antigen-specific B cells (Scott et al. 1980, Pillai et al. 1984). These studies have allowed us to compare hapten-specific cells derived from normal, primed or tolerant individuals in terms of their ability to be stimulated by a variety of known B-cell activators (antigen, anti-Ig, mitogen, T-cell factors) as measured by increase in cell size, hyper-Ia expression, entry in the S phase of the cell cycle, and the appearance of antibody-secreting cells. The low frequency of antigen-specific B cells, and thus the difficulty in obtaining large numbers of cells, has severely limited our ability to examine early
Does cost‐effectiveness play a role in clinical trials?

ABSTRACT: Cost is a measure of resources consumed. By assessing costs, pharmacoeconomic studies complement studies of efficacy and safety, helping to determine the relationships of treatment and outcome. Costs are divided into three categories, including direct cost (resources attributable to the intervention), indirect cost (resulting from reduced productivity), and intangible cost (incurred from pain and emotional suffering). Insurance companies, patients, doctors, and the society all have different perspectives with respect to costs. The present authors review different types of cost analyses and their use in studies of psoriasis, atopic dermatitis, and basal cell carcinoma treatment. Cost studies influence clinicians, policy makers, and third‐party payers’ decisions regarding the implementation of particular therapies or programs. Collection of cost data needs to be incorporated into clinical trials to facilitate these decisions.
Laser-assisted bumping for flip chip assembly

A novel laser-assisted chip bumping technique is presented in which bumps are fabricated on a carrier and subsequently transferred onto silicon chips by a laser-driven release process. Copper bumps with gold bonding layers and intermediate nickel barriers are fabricated on quartz wafers with pre-deposited polyimide layers, using UV lithography and electroplating. The bumps are thermosonically bonded to their respective chips and then released from the carrier by laser machining of the polyimide layer, using light incident through the carrier. Bumps of 60 to 85 /spl mu/m diameter and 50 /spl mu/m height at a pitch of 127 /spl mu/m have been fabricated in peripheral arrays. Parallel bonding and subsequent transfer of arrays of 28 bumps onto test chips have been successfully demonstrated. Individual bump shear tests have been performed on a sample of 13 test chips, showing an average bond strength of 26 gf per bump.
DNA Differential Diagnosis of Human Taeniid Cestodes by Base Excision Sequence Scanning Thymine-Base Reader Analysis with Mitochondrial Genes

ABSTRACT For DNA differential diagnosis of human Taenia cestodes, a base excision sequence scanning thymine-base method using the cytochrome c oxidase subunit I and cytochrome b genes as targets was used. The characteristic thymine-base peak profiles provide four distinct types, unique for T. saginata, T. asiatica, and two genotypes of T. solium. This approach provides a useful tool for the identification and diagnosis of human taeniid cestodes without DNA sequencing if nucleotide sequence databases are available.
Optimal reproductive-skew models fail to predict aggression in wasps

Optimal–skew models (OSMs) predict that cooperative breeding occurs as a result of dominants conceding reproductive benefits to subordinates, and that division of reproduction within groups reflects each cooperator's willingness and ability to contest aggressively for dominance. Polistine paper wasps are a leading model system for testing OSMs, and data on reproduction and aggression appear to support OSMs. These studies, however, measure aggression as a single rate rather than by the activity patterns of individuals. This leads to a potential error: if individuals are more likely to receive aggression when active than when inactive, differences in aggression across samples can reflect changes in activity rather than hostility. This study replicates a field manipulation cited as strongly supporting OSMs. We show that fundamentally different conclusions arise when controlling for individual activity states. Our analyses strongly suggest that behaviours classified as ‘aggression’ in paper wasps are unlikely to function in establishing, maintaining or responding to changes in reproductive skew. This illustrates that OSM tests using aggression or other non–reproductive behaviour as a metric for reproductive partitioning must demonstrate those links rather than assume them.
Mindfulness-Action Based Cognitive Behavioral Therapy for Concurrent Binge Eating Disorder and Substance Use Disorders

Individuals with Binge Eating Disorder (BED) often evidence comorbid Substance Use Disorders (SUD), resulting in poor outcome. This study is the first to examine treatment outcome for this concurrent disordered population. In this pilot study, 38 individuals diagnosed with BED and SUD participated in a 16-week group Mindfulness-Action Based Cognitive Behavioral Therapy (MACBT). Participants significantly improved on measures of objective binge eating episodes; disordered eating attitudes; alcohol and drug addiction severity; and depression. Taken together, MACBT appears to hold promise in treating individuals with co-existing BED-SUD.
Analytical study of a slotted CSMA protocol on a two-state Markov channel

The paper presents an analytical investigation of a simple medium access scheme for wireless networks based on a channel sensing and a retransmission scheme, used to recover packet errors due to wrong channel. The system is slotted and the channel has been modeled by means of a two-state Markov chain to simulate bursty errors. For the sake of simplicity, the case of zero round-trip delay and of reliable feedback channel has been taken into account. The theory developed has the aim to evaluate the queue state evolution of the generic node in the network, in order to estimate the average number of packets-present in the queue in the generic slot and the average time to transmit a packet System simulations have been performed to validate the analytical approach.
Simultaneous Immunodetection of Anthrax, Plague, and Tularemia from Blood Cultures by Use of Multiplexed Suspension Arrays

ABSTRACT Multiplexed detection technologies are becoming increasingly important given the possibility of bioterrorism attacks, for which the range of suspected pathogens can vary considerably. In this work, we describe the use of Luminex MagPlex magnetic microspheres for the construction of two multiplexed diagnostic suspension arrays, enabling antibody-based detection of bacterial pathogens and their related disease biomarkers directly from blood cultures. The first 4-plex diagnostic array enabled the detection of both anthrax and plague infections using soluble disease biomarkers, including protective antigen (PA) and anthrax capsular antigen for anthrax detection and the capsular F1 and LcrV antigens for plague detection. The limits of detection (LODs) ranged between 0.5 and 5 ng/ml for the different antigens. The second 2-plex diagnostic array facilitated the detection of Yersinia pestis (LOD of 1 × 106 CFU/ml) and Francisella tularensis (LOD of 1 × 104 CFU/ml) from blood cultures. Inoculated, propagated blood cultures were processed (15 to 20 min) via 2 possible methodologies (Vacutainer or a simple centrifugation step), allowing the direct detection of bacteria in each sample, and the entire assay could be performed in 90 min. While detection of bacteria and soluble markers from blood cultures using PCR Luminex suspension arrays has been widely described, to our knowledge, this study is the first to demonstrate the utility of the Luminex system for the immunodetection of both bacteria and soluble markers directly from blood cultures. Targeting both the bacterial pathogens as well as two different disease biomarkers for each infection, we demonstrated the benefit of the multiplexed developed assays for enhanced, reliable detection. The presented arrays could easily be expanded to include antibodies for the detection of other pathogens of interest in hospitals or labs, demonstrating the applicability of this technology for the accurate detection and confirmation of a wide range of potential select agents.
Novel Lipid Droplet–Associated Serine Hydrolase Regulates Macrophage Cholesterol Mobilization

Objective—Lipid-laden macrophages or foam cells are characterized by massive cytosolic lipid droplet (LD) deposition containing mostly cholesterol ester (CE) derived from the lipoproteins cleared from the arterial wall. Cholesterol efflux from foam cells is considered to be atheroprotective. Because cholesterol is effluxed as free cholesterol, CE accumulation in LDs may limit free cholesterol efflux. Our objective was to identify proteins that regulate cholesterol trafficking through LDs. Approach and Results—In a proteomic analysis of the LD fraction of RAW 264.7 macrophages, we identified an evolutionarily conserved protein with a canonical GXSXG lipase catalytic motif and a predicted &agr;/&bgr;-hydrolase fold, the RIKEN cDNA 1110057K04 gene, which we named LD-associated hydrolase (LDAH). LDAH association with LDs was confirmed by immunoblotting and immunocytochemistry. LDAH was labeled with a probe specific for active serine hydrolases. LDAH showed relatively weak in vitro CE hydrolase activity. However, cholesterol measurements in intact cells supported a significant role of LDAH in CE homeostasis because LDAH upregulation and downregulation decreased and increased, respectively, intracellular cholesterol and CE in human embryonic kidney-293 cells and RAW 264.7 macrophages. Mutation of the putative nucleophilic serine impaired active hydrolase probe binding, in vitro CE hydrolase activity, and cholesterol-lowering effect in cells, whereas this mutant still localized to the LD. LDAH upregulation increased CE hydrolysis and cholesterol efflux from macrophages, and, interestingly, LDAH is highly expressed in macrophage-rich areas within mouse and human atherosclerotic lesions. Conclusions—The data identify a candidate target to promote reverse cholesterol transport from atherosclerotic lesions.
Parallel program development and execution in the grid

Integration of P-GRADE with Condor and parallel check-pointing results in an environment under which both interactive parallel program development and batch mode execution is possible for the Grid. The integrated P-GRADE/Condor Grid system will guarantee reliable, fault-tolerant parallel program execution in the Grid, like the Condor system guarantees such features for sequential programs. The GRM/PROVE performance monitoring and visualisation toolset has been separated from P-GRADE and extended towards the Grid to create the basis of a general Grid application monitoring infrastructure for parallel programs.
Subscores of the FAB differentiate frontotemporal lobar degeneration from AD

Objective: To determine the clinical utility of the Frontal Assessment Battery (FAB), a short test of frontal lobe functions, in differentiating frontotemporal lobar degeneration (FTLD) from Alzheimer disease (AD). Methods: FAB total scores and subscores for 23 subjects with FTLD and 31 subjects with AD were compared for sensitivity, specificity, and positive predictive value. Concurrent validity of the FAB with the Mini-Mental State Examination (MMSE) and other scales was also assessed. Results: The FAB did not have positive predictive value for FTLD. Total FAB scores did not differ between the FTLD and AD groups. However, three subtests of the FAB (mental flexibility, motor programming, and environmental autonomy) demonstrated significant differences between the two groups. Total FAB scores correlated with scores on the MMSE, a more general test of cognition. Conclusion: The Frontal Assessment Battery did not discriminate subjects with frontotemporal lobar degeneration from those with Alzheimer disease, though certain subtests may be helpful in differential diagnosis.
Spectral Efficiency of Superimposed Pilots in Uplink Massive MIMO Systems

Massive multiple-input multiple-output (MIMO) is a viable technology to improve the spectral efficiency (SE) by spatially multiplexing several users. A potential limitation of Massive MIMO in multicell systems is pilot contamination, which arises from interference in the channel estimation due to the reuse of pilot sequences in neighboring cells. A standard method to reduce pilot contamination, referred to as regular pilot (RP), is to adjust the length of the pilot sequences while transmitting data and pilot symbols disjointly. Alternatively, the superimposed pilot (SP) method sends a superposition of pilot and data symbols, thereby allowing the use of longer pilots which can also reduce pilot contamination. This work considers the uplink of a general multicell Massive MIMO system with SP and maximum ratio combining and derives rigorous closed-form achievable rates, which are used to make comparisons with RP. Numerical results consider a realistic random base station deployment and show that with SP the reduction of pilot contamination is outweighed by the additional coherent and non-coherent interference from the data transmission. Moreover, it turns out that, when the pilot length is optimized, RP provides comparable SE as with SP.
Chitin Induces Natural Competence in Vibrio cholerae

The mosaic-structured Vibrio cholerae genome points to the importance of horizontal gene transfer (HGT) in the evolution of this human pathogen. We showed that V. cholerae can acquire new genetic material by natural transformation during growth on chitin, a biopolymer that is abundant in aquatic habitats (e.g., from crustacean exoskeletons), where it lives as an autochthonous microbe. Transformation competence was found to require a type IV pilus assembly complex, a putative DNA binding protein, and three convergent regulatory cascades, which are activated by chitin, increasing cell density, and nutrient limitation, a decline in growth rate, or stress.
Solving the Inferential Frame Problem in the General Game Description Language

The Game Description Language GDL is the standard input language for general game-playing systems. While players can gain a lot of traction by an efficient inference algorithm for GDL, state-of-the-art reasoners suffer from a variant of a classical KR problem, the inferential frame problem. We present a method by which general game players can transform any given game description into a representation that solves this problem. Our experimental results demonstrate that with the help of automatically generated domain knowledge, a significant speedup can thus be obtained for the majority of the game descriptions from the AAAI competition.
Two-Section Cascaded Coupled Line Wilkinson Power Divider for Dual-Band Applications

A coupled line based design method is presented for the dual-band operation of the Wilkinson power divider. It is shown that a simple two-section cascaded coupled line structure is capable of the dual-band operation in the Wilkinson power divider. The overall divider structure can be simplified with the small number of transmission line sections and the coupled line using the proposed approach.
Usability of Three Electroencephalogram Headsets for Brain-Computer Interfaces: A Within Subject Comparison

Currently the field of brain–computer interfacing is increasingly focused on developing usable brain–computer interfaces (BCIs) to better ensure technology transfer and acceptance. Many studies have investigated the usability of BCI applications as a whole. Here we aim to investigate one specific component of an electroencephalogram (EEG)-based BCI system: the acquisition component. This study compares on the usability of three different EEG headsets in the context of a P300-based BCI application for communication. Thirteen participants took part in a within-subject experiment. Participants were randomly given a Biosemi, Emotiv EPOC or g.Sahara headset. After every session offline classification accuracy (efficacy) was calculated and usability factors (perceived efficiency and user satisfaction) were measured using questionnaires. The 32-channel Biosemi headset offered the highest accuracy (88.5%) compared with the 8-channel g.Sahara (62.7%) and the 14-channel Emotiv (61.7%). There was no difference in accuracy between the Biosemi and the g.Sahara when comparing the same 8 channels. The Biosemi and g.Sahara were rated as more comfortable than the Emotiv. The Emotiv was rated as best for aesthetics. System setup time was highest for the Biosemi headset when compared with the g.Sahara and the Emotiv. Without information about the effectiveness, participants preferred the Emotiv. We recommend the use of a gelled headset for applications which require high accuracy and efficiency and water-based or dry headsets when aesthetics, easy setup and fun are important.
Structural Features of the Central Cannabinoid CB1 Receptor Involved in the Binding of the Specific CB1 Antagonist SR 141716A (*)

The antagonist SR 141716A has a high specificity for the central CB1 cannabinoid receptor and negligeable affinity for the peripheral CB2 receptor, making it an excellent tool for probing receptor structure-activity relationships. From binding experiments with mutated CB1 and with chimeric CB1/CB2 receptors we have begun to identify the domains of CB1 implicated in the recognition of SR 141716A. Receptors were transiently expressed in COS-3 cells, and their binding characteristics were studied with SR 141716A and with CP 55,940, an agonist recognized equally well by the two receptors. The region delineated by the fourth and fifth transmembrane helices of CB1 proved to be crucial for high affinity binding of SR 141716A. The CB1 and CB2 second extracellular loops, e2, were exchanged, modifications that had no effect on SR 141716A binding in the CB1 variant but that eliminated CP 55,940 binding in both mutants. The replacement of the conserved cysteine residues in e2 of CB2 by serine also eliminated CP 55,940 binding, but replacement of those in CB1 resulted in the sequestration of the mutated receptors in the cell cytoplasm. The e2 domain thus plays some role in CP 55,940 binding but none in SR 141716A recognition, binding of the latter clearly implicating residues in the adjoining transmembrane helices.
The binding of Maize DHN1 to Lipid Vesicles. Gain of Structure and Lipid Specificity1

Dehydrins (DHNs; late embryogenesis abundant D-11) are a family of plant proteins induced in response to abiotic stresses such as drought, low temperature, and salinity or during the late stages of embryogenesis. Spectral and thermal properties of these proteins in purified form suggest that they are “intrinsically unstructured. ” However, DHNs contain at least one copy of a consensus 15-amino acid sequence, the “K segment,” which resembles a class A2 amphipathic α-helical, lipid-binding domain found in other proteins such as apolipoproteins and α-synuclein. The presence of the K segment raises the question of whether DHNs bind lipids, bilayers, or phospholipid vesicles. Here, we show that maize (Zea mays) DHN DHN1 can bind to lipid vesicles that contain acidic phospholipids. We also observe that DHN1 binds more favorably to vesicles of smaller diameter than to larger vesicles, and that the association of DHN1 with vesicles results in an apparent increase of α-helicity of the protein. Therefore, DHNs, and presumably somewhat similar plant stress proteins in the late embryogenesis abundant and cold-regulated classes may undergo function-related conformational changes at the water/membrane interface, perhaps related to the stabilization of vesicles or other endomembrane structures under stress conditions.
Comparison of HbA1 and Fructosamine in Diagnosis of Glucose-Tolerance Abnormalities

Total glycosylated hemoglobin (HbA1) and fructosamine were evaluated as screening tools for detection of glucose-tolerance abnormalities in 144 asymptomatic subjects undergoing a 75-g oral glucose tolerance test. Subjects were classified according to World Health Organization criteria as having normal (n = 78), impaired (n = 40), or diabetic (n = 26) glucose tolerance. We found good specificity for HbA1 and fructosamine (100 and 97%, respectively) but low sensitivity (15 and 19%, respectively). At the intersection of the curves of sensitivity and specificity drawn from various thresholds of normality, both sensitivity and specificity were 75% for HbA1 and 55% for fructosamine. Thus, neither HbA1 nor fructosamine seems to be suitable for the diagnosis of mild abnormalities in glucose tolerance.