Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular Phenotypes in Cultured Maple Syrup Urine Disease Cells

The activity of the branched-chain alpha-keto acid dehydrogenase complex is deficient in patients with the inherited maple syrup urine disease (MSUD). To elucidate the molecular basis of this metabolic disorder, we have isolated three overlapping cDNA clones encoding the E1 alpha subunit of the human enzyme complex. The composite human E1 alpha cDNA consists of 1783 base pairs encoding the entire human E1 alpha subunit of 400 amino acids with calculated Mr = 45,552. The human E1 alpha and the previously isolated human E2 cDNAs were used as probes in Northern blot analysis with cultured fibroblasts and lymphoblasts from seven unrelated MSUD patients. The results along with those of Western blotting have revealed five distinct molecular phenotypes according to mRNA and protein-subunit contents. These consist of type I, where the levels of E1 alpha mRNA and E1 alpha and E1 beta subunits are normal in cells, but E1 activity is deficient; Type II, where the E1 alpha mRNA is present in normal quantity, whereas the contents of E1 alpha and E1 beta subunits are reduced; Type III, where the level of E1 alpha mRNA is markedly reduced with a concomitant loss of E1 alpha and E1 beta subunits; Type IV, where the contents of both E2 mRNA and E2 subunits are markedly reduced; and Type V, where the E2 mRNA is normally expressed, but the E2 subunit is markedly reduced or completely absent. Type V includes thiamin-responsive (WG-34) and certain classical MSUD cells. These molecular phenotypes have demonstrated the complexity of MSUD and identified the affected gene in different patients for further characterization.
Discontinuation of Lamivudine Treatment in HBeAg-Negative Chronic Hepatitis B: A Pilot Study with Long-Term follow-up

Background Finite treatment of hepatitis B e antigen (HBeAg)-negative chronic hepatitis B (CHB) with nucleoside/nucleotide analogues (NAs) is important in resource-limited countries. Outcome of treatment discontinuation in patients on long-term lamivudine (LVD) was assessed in a single centre observational pilot study in the current study. Methods Non-cirrhotic patients on LVD for at least 5 years with undetectable HBV DNA on at least two consecutive assessments were offered to stop treatment. Biochemical, serological and virological measures were determined at 3–6 month intervals after treatment discontinuation. Serum quantitative hepatitis B surface antigen (HBsAg) was determined at treatment discontinuation and 5–6 years thereafter. NA treatment was re-instituted in patients with confirmed viral rebound defined as HBV DNA >20,000 IU/ml. Relapser patients were no longer followed but were re-assessed 6 years after treatment cessation. Results LVD was discontinued in 23 patients. 8 patients relapsed within 1 year and NA treatment was restarted; 15 patients (65%) were non-relapsers. Thirteen of them were followed for at least 5 years. Two patients had undetectable HBV DNA throughout the follow-up period. In the rest, HBV DNA fluctuated at low levels. Two patients cleared HBsAg 24 and 36 months after stopping treatment. Quantitative HBsAg levels 5–7 years after treatment discontinuation were lower in non-relapser compared to relapser patients (1.21 IU/ml ±0.98 versus 2.71 ±0.76; P=0.002). Of 8 relapser patients 1 patient had HBsAg levels less than 100 IU/ml compared to 11 out of 13 non-relapser patients (P=0.0022). Conclusions These data suggest that cessation of NA treatment is a viable option after a reasonable treatment duration in patients with HBeAg-negative CHB and that HBsAg clearance may become an achievable target in these patients.
Fos-dependent induction of Chk1 protects osteoblasts from replication stress

Stable Fos expression in the osteoblast lineage results in the development of osteosarcomas (OS) in mice, yet the underlying mechanisms are poorly understood. Using a genetic system in which Fos expression can be induced in osteoblasts in a doxycycline-dependent manner and through subsequent RNA sequencing and gene set enrichment analysis, we were able to identify novel transcriptional targets of Fos in osteoblasts. These included a distinct activation of cellular response toward replication stress (RS), exemplified by a Fos-dependent induction of the RS-suppressing Chk1 kinase. Importantly, Fos expression protects osteoblasts from RS and DNA damage likely through upregulation of Chk1 and facilitates transformation by Ras/E1A oncogenes. These data reveal a novel function of Fos in safeguarding genome stability during replication, which is particularly relevant in conditions of oncogene-induced S-phase entry.
Preservar los puentes: historia de la conservación patrimonial de la ingeniería civil en España (Siglo XVI-1936)

A partir de una exhaustiva busqueda archivistica y bibliografica, este libro plantea una historia hasta la fecha inedita, la de la conservacion patrimonial de la ingenieria civil en Espana. Se traza un amplio recorrido, desde el Renacimiento hasta principios del siglo XX, y se abordan actuaciones, muchas desconocidas, en obras emblematicas. Con ello, se enriquece la historia de la conservacion y la restauracion del patrimonio en Espana, y se contribuye al reconocimiento del extraordinario legado de la ingenieria civil.
Industrial Automation System on Device (IndASoD) -A device modeling industrial automation scenarios

Power System Automation which includes automation of generation, transmission and distribution of electricity are undergoing evolution or modernization. Electric Utilities have a long tradition of owning and controlling the communication networks for mission critical applications because of the concerns about reliability, safety, security and cost. The convergence of these communication networks with the internet and open communication standards leads to new challenges like the finding of faults in these systems requires new skills and capabilities. This evolution necessitates system/tools for early diagnostics and analysis of communication subsystem. The paper discusses the automation system perspectives of Quality of Control (QoC) and Loss of Visibility to arrive at a relationship between scenarios and communication traffic flows. This relationship serves as the basis of building a device which has Industrial Automation Scenarios inbuilt, termed - Industrial Automation System on Device (IndASoD). The architecture of the device includes combining innovative models and simulators in mainstream communication research and leveraging emerging technology platforms like Software Defined Networks (SDN). The paper also discusses the feasibility of such a device in the context of modernization scenarios like upgrade and technology evaluations.
Nearly antiferromagnetic Fermi-liquid description of magnetic scaling and spin-gap behavior.

We present a Fermi-liquid description of magnetic scaling and spin-gap behavior in strongly correlated electron systems. We show that a gap in the spin-excitation spectrum is a natural consequence of the existence of a second energy scale of magnetic origin, in systems of itinerant, but nearly localized electron spins, such as are found in the cuprate superconductors and heavy-electron systems. A second energy scale leads to a frequency-dependent restoring force [ital f][sup [ital a]]([bold q],[omega]) that becomes stronger as the frequency increases. A spin gap at [bold Q]=([pi]/[ital a],[pi]/[ital a]) is a consequence of these frequency-dependent vertex corrections; it takes on a constant value when the antiferromagnetic correlation length becomes a constant. We show that the spin-spin response function reduces to that recently introduced by Barzykin to describe the damping by particle-hole excitations of the spin-wave spectrum found in the [ital N]=[infinity] quantum nonlinear [sigma] model. We discuss fully doped and underdoped cuprate superconductors, the appearance of a pseudogap of magnetic origin in the quasiparticle density of states in the underdoped system, and the constraints nuclear magnetic resonance and neutron-scattering experiments impose on the magnitude and temperature dependence of the Fermi-liquid parameters.
Une carte scientométrique de la recherche en éducation vue par la base de données internationales Scopus

La recherche en education couvre un territoire de divers sujets, ce qui presente un defi d’integration. Nous avons effectue une analyse bibliographique de la base de donnees internationale Scopus afin de decrire la recherche autour du fait educatif. Nous avons construit une carte globale – essentiellement de la recherche anglophone – consistant en dix-huit clusters distincts connectes par des ensembles de ressources bibliographiques. Les clusters different dans leurs references partagees et dans la facon dont ils se focalisent sur un aspect de la recherche en education. Certains clusters partagent des references dont les publications sont tres connectees et portent directement sur l’enseignement et l’apprentissage (par exemple, le cluster Learning) alors que d’autres clusters partagent des references de maniere moins connectee et s’interessent a des sujets qui se trouvent a l’intersection des recherches autour du fait educatif et d’autres champs d’investigation (par exemple, le cluster Sociology of Education).
Transient global amnesia as a manifestation of transient cerebral ischemia

Ten patients with transient global amnesia (TGA) associated with symptoms of transient focal cerebral ischemia were seen at the University Department of Neurology, Århus Kommunehospital in the period 1966–1978. All had either prior to or following the amnesic attack transient ischemic attacks (TIA) in the territory of the posterior cerebral circulation. On admission minor neurological deficits were noted in three and normal findings in the remaining seven. There was no evidence of epilepsy in any case.
Unified Wide-Speed Sensorless Scheme Using Nonlinear Optimization for IPMSM Drives

This paper proposes a novel unified nonlinear optimization-based speed and position estimation algorithm for interior permanent magnet synchronous motor drives at wide speed range operations. A cost function based on the voltage equations in the stationary reference frame is employed for speed and position estimation. The speed and position can be estimated by minimizing the cost function. At low speed, including the standstill condition, the cost function is modified and high-frequency sinusoidal voltage signals are injected in the estimated magnetic axis. A phase locked loop is combined with the proposed position estimator for reducing the noise of estimation results. Compared with existing sensorless methods, a unified estimator is used at low- and high-speed operations and a better performance is obtained in transient and steady-state conditions. The convexity of the cost functions with respect to the speed and position estimation errors is analyzed in the paper. The feasibility of the proposed estimation algorithm is validated with an experimental test bench.
Vesicle Docking in Regulated Exocytosis

In electron micrographs, many secretory and synaptic vesicles are found ‘docked’ at the target membrane, but it is unclear why and how. It is generally assumed that docking is a necessary first step in the secretory pathway before vesicles can acquire fusion competence (through ‘priming’), but recent studies challenge this. New biophysical methods have become available to detect how vesicles are tethered at the target membrane, and genetic manipulations have implicated many genes in tethering, docking and priming. However, these studies have not yet led to consistent working models for these steps. In this study, we review recent attempts to characterize these early steps and the cellular factors to orchestrate them. We discuss whether assays for docking, tethering and priming report on the same phenomena and whether all vesicles necessarily follow the same linear docking–priming–fusion pathway. We conclude that most evidence to date is consistent with such a linear pathway assuming several refinements that imply that some vesicles can be nonfunctionally docked (‘dead‐end’ docking) or, conversely, that the linear pathway can be greatly accelerated (crash fusion).
An industrial fault injection platform for soft-error dependability analysis and hardening of complex system-on-a-chip

This paper presents a fault injection platform that is currently developed and used to perform soft-error dependability analysis and hardening of complex SoCs1. Primarily, it is oriented toward safety analysis, safety requirement conformance testing and hardening of complex SoCs. This platform makes use of clusters of hardware emulation resources available for SoC verification to achieve massive faults injection capabilities. It is able to distribute fault injection campaigns across multiple heterogeneous emulation platforms to achieve high fault coverage. It is able to virtually handle almost any circuit size and is designed to support all kind of designs. We present the first results obtained on a small design, the Leon2 IP, on which exhaustive fault injection have been performed.
Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development

Dopamine (DA)‐producing neurons in the ventral midbrain are generated from a specified neuronal lineage and form selective axonal pathways that mediate multiple CNS functions. Expression of the gene encoding tyrosine hydroxylase (TH), which is a key enzyme of catecholamine biosynthesis, is regulated during the development of midbrain DA neurons. In the present study, we report the developmental regulation and cell type specificity of TH gene promoter in the ventral midbrain by using a green fluorescent protein (GFP) reporter system. Transgenic mice were generated that express GFP in the majority of midbrain DA neurons under the control of the 9‐kb upstream region of the rat TH gene. At an early embryonic stage, GFP expression was induced in the developing DA neurons, and the expression was then markedly down‐regulated at later embryonic stages. However, the expression was reactivated and approached the adult levels during early post‐natal development. These developmental changes in GFP expression patterns suggest the presence of multistep regulatory mechanisms for TH gene expression during DA neuron development. The TH promoter appears to possess transcriptional elements at least necessary for the induction of TH expression at the early embryonic stage and its reactivation during the post‐natal development.
Structural basis of the recognition of a methylated histone tail by JMJD2A

The Jumonji C domain is a catalytic motif that mediates histone lysine demethylation. The Jumonji C-containing oxygenase JMJD2A specifically demethylates tri- and dimethylated lysine-9 and lysine-36 of histone 3 (H3K9/36me3/2). Here we present structures of the JMJD2A catalytic core complexed with methylated H3K36 peptide substrates in the presence of Fe(II) and N-oxalylglycine. We found that the interaction between JMJD2A and peptides largely involves the main chains of the enzyme and the peptide. The peptide-binding specificity is primarily determined by the primary structure of the peptide, which explains the specificity of JMJD2A for methylated H3K9 and H3K36 instead of other methylated residues such as H3K27. The specificity for a particular methyl group, however, is affected by multiple factors, such as space and the electrostatic environment in the catalytic center of the enzyme. These results provide insights into the mechanisms and specificity of histone demethylation.
Production in FL Cells of Infectious and Potentially Infectious Reovirus

Spendlove, Rex S. (California State Department of Public Health, Berkeley), Edwin H. Lennette, Charles O. Knight, and Jean N. Chin. Production in FL cells of infectious and potentially infectious reovirus. J. Bacteriol. 92:1036–1040. 1966. —A comparative study was made of the development in, and release from, FL cells of infectious and potentially infectious (chymotrypsin-activatable) reovirus (Lang strain). The latent period was shorter, the rate of synthesis was more rapid, and the total yield was more than 10-fold greater in potentially infectious virus as compared with infectious virus. Almost all of the potentially infectious virus, but only approximately one-third of the infectious virus, was released from the infected cells.
Magnetization performance of BP-Based algorithm

In this paper, we present the performance of the Belief Propagation-Based (BP-Based) algorithm as a function of the magnetization. Previous works, have shown that the BP algorithm, is equivalent to the Thouless- Anderson-Palmer(TAP) [1] approach, we develop the log-likelihood ratios-Belief Propagation (LLR-BP) algorithm and its simplification, the BP-Based algorithm using this method.
Clinical and life quality differences between Mexican American diabetic patients at a free clinic and a hospital-affiliated clinic in Texas.

OBJECTIVE This paper explores the contribution of personal, cultural, and disease characteristics, diabetes knowledge, number of symptoms, and 2 specific clinics to the explained variance in glycosylated hemoglobin (A1c) and quality of life among Mexican Americans with type 2 diabetes. DESIGN This descriptive correlational study used hierarchical multiple regression analysis. SAMPLE 87 Mexican American patients with type 2 diabetes were recruited from 2 clinics, 1 hospital-affiliated and 1 free clinic, that served a primarily minority and indigent population. MEASUREMENTS A language-based acculturation scale, the Diabetes Knowledge Questionnaire-24, Diabetes Symptom Self-Care Inventory, and Self-Anchoring Striving Scale were administered in one-on-one interviews. Medical records were reviewed for recent A1c and health history. RESULTS The participants' clinic explained an additional 13% of the variance in A1c and 6% of the variance in quality of life after controlling for gender, acculturation, time since diagnosis, number of diabetes medications, diabetes knowledge, and number of symptoms. Clinics differed in patient characteristics, beyond the variables entered in the regression model, and in their care delivery. CONCLUSIONS Clinic characteristics and their influence on diabetes outcomes should be explored to maximize patients' abilities to steer the course of their diabetes away from complications.
Numerical simulations of scattering from time-varying, randomly rough surfaces

The literature has seen the development of robust and efficient numerical techniques for exact calculations of rough surface scattering. We discuss how such methods, typically formulated for time-independent surfaces, can be extended to calculate scattering from time-evolving ocean-like surfaces. Estimates are provided for the choice of parameters in such time-varying simulations. The method of ordered multiple interactions (MOMI) is used to calculate time-varying scattering from surfaces generated according to linear and nonlinear (Creamer) models for incidence angles ranging from normal to low grazing. We discuss the runtime considerations and demonstrate that combining the MOMI with a fast multipole method (FMM)-type acceleration technique makes large-scale time-varying Monte Carlo simulations possible. The average Doppler spectra of backscattered signals obtained from such simulations are compared for different incident angles, polarizations, and surface models. In particular, the simulations show a broadening of the Doppler spectra for nonlinear surfaces, especially at low grazing angles (LGA) and a separation of the vertical and horizontal polarization spectra at LGA for nonlinear surfaces. This spectral separation at LGA is not observed when the linear surfaces are used.
PC VIPR: a high-speed 3D phase-contrast method for flow quantification and high-resolution angiography.

BACKGROUND AND PURPOSE Three-dimensional phase-contrast (3DPC) is limited by long imaging times, limited coverage, flow artifacts, and the need to perform multiple additional 2D examinations (2DPC) to measure flow. A highly undersampled 3D radial acquisition (isotropic-voxel radial projection imaging [PCVIPR]) makes it possible to increase the product of volume coverage and spatial resolution by a factor of 30 for the same imaging time as conventional Cartesian 3DPC. This provides anatomic information over a large volume with high isotropic resolution and permits retrospective measurement of average flow rates throughout the volume. METHODS PCVIPR acquires a reference and three flow-encoded acquisitions for each VIPR projection. Complex difference images were formed by combining information from all flow directions. Following retrospective definition of planes perpendicular to selected vessels, volume flow rates were determined by using phase-difference information. The accuracy of average flow measurement was investigated in a phantom and in six volunteers. Anatomic PCVIPR images acquired in three patients and three volunteers by using a 384(3) matrix were compared with conventional Cartesian 3DPC. RESULTS The flow validation produced R2 = 0.99 in vitro and R2 = 0.97 in vivo. PCVIPR produced minimal streak and pulsatile flow artifacts. PCVIPR produced far higher resolution and volume coverage in comparable imaging times. The highest acceleration factors relative to 3DPC were achieved by using gadolinium-contrast material. Ultimately, acceleration factors are limited by signal-to-noise ratio. CONCLUSION PCVIPR rapidly provides isotropic high-resolution angiographic images and permits retrospective measurement of average flow rate throughout the volume without the need to prescribe multiple 2D acquisition planes.
Valorización de lodos de depuradora como fertilizante en el marco de la economía circular: De residuo a recurso

La escasez futura de fertilizantes minerales y el aumento de sus costes economicos y ambientales, son razones clave para promover el uso de los residuos organicos biodegradables, entre ellos los lodos de depuradora, como fertilizantes en la agricultura. Ademas la produccion de lodos de depuradora se ha incrementado en los ultimos anos con la obligacion de depurar las aguas residuales desde 2005 en Europa. Por ello, es importante tener en cuenta los metales pesados presentes en los mismos, comparando las implicaciones de los valores limite actuales y el analisis de las implicaciones de establecer limites mas estrictos por la Union Europea (EU), para evaluar el efecto sobre la disponibilidad de sitios para el uso agricola de lodos en Galicia. Los resultados indican que mas del 90% de los suelos gallegos analizados son adecuados para recibir lodos con la actual normativa R.D. 1310/90, pero menos del 30% cumplirian con el ultimo borrador de modificacion de la normativa europea. Posteriormente es necesario valorar el comportamiento de la aplicacion de lodos en los suelos disponibles, teniendo en cuenta el metal pesado regulado con la mayor concentracion de lodos de depuracion, que es el zinc, con diferentes escenarios de regulacion, aplicacion, dosis y tipo de lodo, para satisfacer las necesidades del cultivo. Resaltando la conveniencia de utilizar compost como enmienda, y el lodo granulado y el digerido anaerobicamente como fertilizantes, para reducir la posible contaminacion del suelo. En 2015, se aprobo el Paquete sobre Economia Circular para la UE, el cual propone objetivos sobre los residuos organicos, con el fin de poder usarlos como materias primas para la elaboracion de abonos y crear un mercado regulado para su uso en la UE, reduciendo con ello los residuos, el consumo de energia y los danos ambientales. Por ello, es importante caracterizar el efecto de estos abonos o enmiendas organicas, elaborados con lodos de depuradora estabilizados con cal, sobre la fertilidad del suelo y el crecimiento de pastizales. Pudiendo ser una alternativa viable para suplir las necesidades de cal en los pastos y otros cultivos, e incluso parcialmente de fertilizantes minerales, reduciendo los costes de produccion en las explotaciones, y el impacto ambiental de los residuos y los fertilizantes quimicos.