Miracle-dz/UniFAR-FTUs huggingface dataset:
Analysis of aflatoxin regulatory factors in serial transfer-induced non-aflatoxigenic Aspergillus parasiticus

Aflatoxins (AFs) are carcinogenic secondary metabolites of Aspergillus parasiticus. In previous studies, non-toxigenic A. parasiticus sec− (for secondary metabolism negative) variants were generated through serial transfer of mycelia from their toxigenic sec+ (for secondary metabolism positive) parents for genetic and physiological analysis for understanding regulation of AF biosynthesis. Previous studies have shown no difference in the DNA sequence of aflR, a positive regulator of AF production, in the sec+ and sec− strains. In this study, AflJ, another positive regulator of AF production, laeA, a global regulator of secondary metabolism, and the intergenic region between aflR and aflJ, were analysed to determine if they play a role in establishment of the sec− phenotype. The study showed that while this sequence identity extended to the aflJ as well as the aflJ–aflR intergenic region, expression of aflR in the sec− strain was several fold lower than that observed in the sec+ strain, while aflJ expression was barely detectable in the sec− strain. Western blot analysis indicated that despite AflR protein being present in the sec− strain, no toxin production resulted. Introduction of a second copy of aflR into the sec− strain increased aflR expression, but did not restore AF production. Lastly, reverse transcription-PCR analysis revealed that laeA was expressed in both sec+ and sec− strains. These results suggest that although aflR, aflJ and laeA are necessary for AF production, they are not sufficient. We propose that the aflR and aflJ expression may be regulated by element(s) downstream from laeA or from pathways not influenced by laeA.
Speech Source Separation in Convolutive Environments Using Space-Time-Frequency Analysis

We propose a new method for speech source separation that is based on directionally-disjoint estimation of the transfer functions between microphones and sources at different frequencies and at multiple times. The spatial transfer functions are estimated from eigenvectors of the microphones' correlation matrix. Smoothing and association of transfer function parameters across different frequencies are performed by simultaneous extended Kalman filtering of the amplitude and phase estimates. This approach allows transfer function estimation even if the number of sources is greater than the number of microphones, and it can operate for both wideband and narrowband sources. The performance of the proposed method was studied via simulations and the results show good performance.
Energy-efficient quality-of-service-aware cooperation in wireless networks

Conventional cooperative diversity techniques consider a three-node setup, which involves a pair of source-destination nodes and a signal relay node. It is noted that the existing cooperation schemes are performed in a session-based manner in which the role of each node being a source or relay remains unchanged during each session. The radio resource allocation and the associated performance analysis are not jointly performed. As a result, resource utilisation is less efficient. In this paper, an energy-efficient quality-of-service (QoS)-aware cooperative transmission strategy based on joint resource allocation is proposed. Two source nodes with distinct destinations and QoS requirements are simultaneously considered in resource allocation to optimise the energy efficiency. The QoS outage probability is derived analytically and the attainable energy efficiency is investigated for the proposed scheme. Numerical results show that significant performance gain can be achieved for both node pairs with the proposed cooperation scheme.
Antenna Arrays for Line-of-Sight Massive MIMO: Half Wavelength is not Enough

The aim of this paper is to analyze the array synthesis for 5 G massive MIMO systems in the line-of-sight working condition. The main result of the numerical investigation performed is that non-uniform arrays are the natural choice in this kind of application. In particular, by using non-equispaced arrays, we show that it is possible to achieve a better average condition number of the channel matrix and a significantly higher spectral efficiency. Furthermore, we verify that increasing the array size is beneficial also for circular arrays, and we provide some useful rules-of-thumb for antenna array design for massive MIMO applications. These results are in contrast to the widely-accepted idea in the 5 G massive MIMO literature, in which the half-wavelength linear uniform array is universally adopted.
Folding Avoidance in Skeletal Strokes

Skeletal strokes is a method for mapping vector artwork defined along a straight path onto an arbitrary destination path. It is commonly used to give sketches a hand-drawn look, by mapping a scanned natural-medium stroke onto a user-drawn path. When the destination path turns corners or has areas with a small radius of curvature, the mapped artwork takes on an undesirable folded or creased appearance inside the corner or curve. We present a method to eliminate the folds in many cases by adjusting the skeleton before using it to map the artwork. While this cannot address all cases with folding, it gives greatly improved results in the cases that it can address.
Protective Role of Connexin 32 in Steady-State Hematopoiesis, Regeneration State, and Leukemogenesis

The role of gap junctions formed by connexins (Cxs) has been implicated in the homeostatic regulation of multicellular systems. Primitive hematopoietic progenitor cells form a multicellular system, but a previous report states that Cx32 is not expressed in the bone marrow. Thus, a question arises as to why Cx molecules are not detected in the hematopoietic tissue other than in stromal cells. Based on our preliminary study, which suggested a potential impairment of hematopoiesis in Cx32-knockout (KO) mice, the objectives of the present study were to determine whether Cx32 functions in the bone marrow during steady-state hematopoiesis and to examine its possible protective roles during regeneration after chemical abrasions and during leukemogenesis after the administration of a secondary genotoxic chemical, methyl nitrosourea (MNU). As a result, the Cx32 molecule, functioning in the hematopoietic stem cell (HSC) compartment during steady-state hematopoiesis, was observed for the first time; the expressions of Cx32 at the mRNA level, as determined by polymerase chain reaction analysis, and at the protein level, determined using an anti-Cx32 antibody, were observed only in the lin−c-kit+ HSC fraction, using a combination of immunobead-density gradient and immunomagnetic bead separation. Hematopoiesis was impaired in the absence of Cx32, and it was delayed during regeneration after chemical abrasion with 5-fluorouracil at 150 mg/kg body wt in Cx32-KO mice. Cx32-KO mice showed increased leukemogenicity compared with wild-type mice after MNU injection; furthermore, in a competitive assay for leukemogenicity in mice that had been lethally irradiated and repopulated with a mixed population of bone marrow cells from Cx32-KO mice and wild-type mice, the resulting leukemias originated predominantly from Cx32-KO bone marrow cells. In summary, the role of Cx32 in hematopoiesis was not previously recognized, and Cx32 was expressed only in HSCs and their progenitor cells. The results indicate that Cx32 in wild-type mice protects HSCs from chemical abrasion and leukemogenic impacts.
Kinetic models in reverse micelles.

INTRODUCTION Biological studies at the molecular level usually follow a process which starts with the isolation from the cell ofthe macromolecules of interest, for instance proteins and nucleic acids, which are then studied in an in vitro system composed of a buffered aqueous solution. In the study of the catalytic activity of proteins it may be apparent that the isolated enzyme lacks the structured environment in which it usually acts in vivo. The discipline that emerged in the late 1970s, baptized as Micellar Enzymology by Martinek's group [1-3], tries to approach the above problem by using a model system based on the self-organizing properties of amphiphiles in solution; this model system is named reverse micelles. It is hypothesized that this system reliably resembles the microenvironment that enzymes find in the cell: reverse micelles consist of micropools of water lined by a monolayer of an amphiphile, all dispersed in an apolar solvent. For a hydrophilic enzyme, which is usually found in the cytosol or the matrix of an organelle, reverse micelles provide the waterpools as a mimetic environment, where the properties of the water resemble the properties of the water closely associated with the cell [4-7]. Since the size of the protein and the size of waterpools are similar, the latter can resemble, better than a bulk buffered aqueous solution, the structured and viscous environment of the cytosol [8]. Enzymes which interact with membranes as either peripheral or integral proteins also find the appropriate environment in reverse micelles [9-14], provided by the monolayer of surfactant and its closely associated water. Not only can the environment of the protein be modelled in a reverse micellar system, so can that of the substrate [15]. : reverse micelles can host all kinds of substrate molecules whether hydrophilic, hydrophobic or amphiphilic, and this is an important advantage over
Rapid integration of young newborn dentate gyrus granule cells in the adult hippocampal circuitry

Newborn dentate gyrus granule cells (DGCs) are integrated into the hippocampal circuitry and contribute to the cognitive functions of learning and memory. The dendritic maturation of newborn DGCs in adult mice occurs by the first 3–4 weeks, but DGCs seem to receive a variety of neural inputs at both their dendrites and soma even shortly after their birth. However, few studies on the axonal maturation of newborn DGCs have focused on synaptic structure. Here, we investigated the potentiality of output and input in newborn DGCs, especially in the early period after terminal mitosis. We labeled nestin‐positive progenitor cells by injecting GFP Cre‐reporter adenovirus into Nestin‐Cre mice, enabling us to trace the development of progenitor cells by their GFP expression. In addition to GABAergic input from interneurons, we observed that the young DGCs received axosomatic input from the medial septum as early as postinfection day 7 (PID 7). To evaluate the axonal maturation of the newborn DGCs compared with mature DCGs, we performed confocal and electron microscopic analyses. We observed that newborn DGCs projected their mossy fibers to the CA3 region, forming small terminals on hilar or CA3 interneurons and large boutons on CA3 pyramidal cells. These terminals expressed vesicular glutamate transporter 1, indicating they were glutamatergic terminals. Intriguingly, the terminals at PID 7 had already formed asymmetric synapses, similar to those of mature DGCs. Together, our findings suggest that newborn DGCs may form excitatory synapses on both interneurons and CA3 pyramidal cells within 7 days of their terminal mitosis.
Impact of dietary and lifestyle factors on the prevalence of hypertension in Western populations.

BACKGROUND Data on the impact of the many dietary and lifestyle factors on the prevalence of hypertension in Western societies are lacking. This study quantified the contributions of body weight, physical inactivity and dietary factors to the prevalence of hypertension in Finland, Italy, the Netherlands, United Kingdom (UK) and USA. METHODS Distributions of blood pressure (BP) and risk factors in Western populations were obtained from nationwide surveys. The effect of risk factors on BP was assessed by meta-regression analysis of randomized trials, published between 1966 and March 2001. Population attributable risk percentages (PAR%) for hypertension (i.e. systolic BP > or =140 mmHg) were computed for all risk factors in the five countries. RESULTS Being overweight made the largest contribution to hypertension, with PAR% between 11% (Italy) and 25% (USA). PAR% were 5-13% for physical inactivity, 9-17% for high sodium intake, 4-17% for low potassium intake and 4-8% for low magnesium intake. The impact of alcohol was small (2-3%) in all populations. PAR% varied among populations for inadequate intake of calcium (2-8%), magnesium (4-8%), coffee (0-9%) and fish fatty acids (3-16%). CONCLUSIONS Diet and lifestyle have a substantial impact on hypertension in Western societies, with being overweight, physical inactivity, high sodium intake and low potassium intake being the main contributors. The relative significance of different risk factors varies among populations, which is important for preventative strategies.
Acetochlor in the Hydrologic System in the Midwestern United States, 1994

The herbicide acetochlor [2-chloro-N-(ethoxymethyl)-N-(2-ethyl-6-methylphenyl)acetamide] was given conditional registration in the United States by the U.S. Environmental Protection Agency in March 1994. This registration provided a rare opportunity to investigate the occurrence of a pesticide during its first season of extensive use in the midwestern United States. Water samples collected and analyzed by the U.S. Geological Survey during 1994 documented the distribution of acetochlor in the hydrologic system; it was detected in 29% of the rain samples from four sites in Iowa, 17% of the stream samples from 51 sites across nine states, and 0% of the groundwater samples from 38 wells across eight states. Acetochlor exhibited concentration increases in rain and streams following its application to corn in the midwestern United States, with 75% of the rainwater and 35% of the stream samples having acetochlor detected during this time period. Acetochlor concentrations in rain decreased as the growing season p.
Application of a Simple Multiplex PCR To Aid in Routine Work of the Mycobacterium Reference Laboratory

ABSTRACT A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation ofM. tuberculosis, Mycobacterium bovis, M. bovis BCG, andMycobacterium africanum subtypes I and II. In addition, this approach was incorporated into a multiplex PCR that included primers specific for IS6110 and the 65-kDa antigen gene in order to differentiate members of the M.tuberculosis complex from atypical mycobacteria.
Diverse functional outcomes of Plasmodium falciparum ligation of EPCR: potential implications for malarial pathogenesis

Plasmodium falciparum‐infected erythrocytes (IRBC) expressing the domain cassettes (DC) 8 and 13 of the cytoadherent ligand P. falciparum erythrocyte membrane protein 1 adhere to the endothelial protein C receptor (EPCR). By interfering with EPCR anti‐coagulant and pro‐endothelial barrier functions, IRBC adhesion could promote coagulation and vascular permeability that contribute to the pathogenesis of cerebral malaria. In this study, we examined the adhesion of DC8‐ and DC13‐expressing parasite lines to endothelial cells from different microvasculature, and the consequences of EPCR engagement on endothelial cell function. We found that IRBC from IT4var19 (DC8) and IT4var07 (DC13) parasite lines adhered to human brain, lung and dermal endothelial cells under shear stress. However, the relative contribution of EPCR to parasite cytoadherence on different types of endothelial cell varied. We also observed divergent functional outcomes for DC8 cysteine‐rich interdomain region (CIDR)α1. 1 and DC13 CIDRα1. 4 domains. IT4var07 CIDRα1. 4 inhibited generation of activated protein C (APC) on lung and dermal endothelial cells and blocked the APC–EPCR binding interaction on brain endothelial cells. IT4var19 CIDRα1. 1 inhibited thrombin‐induced endothelial barrier dysfunction in lung endothelial cells, whereas IT4var07 CIDRα1. 4 inhibited the protective effect of APC on thrombin‐induced permeability. Overall, these findings reveal a much greater complexity of how CIDRα1‐expressing parasites may modulate malaria pathogenesis through EPCR adhesion.
Type I IFNs Are Required to Promote Central Nervous System Immune Surveillance through the Recruitment of Inflammatory Monocytes upon Systemic Inflammation

Brain-resident microglia and peripheral migratory leukocytes play essential roles in shaping the immune response in the central nervous system. These cells activate and migrate in response to chemokines produced during active immune responses and may contribute to the progression of neuroinflammation. Herein, we addressed the participation of type I–II interferons in the response displayed by microglia and inflammatory monocytes to comprehend the contribution of these cytokines in the establishment and development of a neuroinflammatory process. Following systemic lipopolysaccharide (LPS) challenge, we found glial reactivity and an active recruitment of CD45hi leukocytes close to CD31+ vascular endothelial cells in circumventricular organs. Isolated CD11b+ CD45hi Ly6Chi Ly6G−-primed inflammatory monocytes were able to induce T cell proliferation, unlike CD11b+ CD45lo microglia. Moreover, ex vivo re-stimulation with LPS exhibited an enhancement of T cell proliferative response promoted by inflammatory monocytes. These myeloid cells also proved to be recruited in a type I interferon-dependent fashion as opposed to neutrophils, unveiling a role of these cytokines in their trafficking. Together, our results compares the phenotypic and functional features between tissue-resident vs peripheral recruited cells in an inflamed microenvironment, identifying inflammatory monocytes as key sentinels in a LPS-induced murine model of neuroinflammation.
Formation of Vascular S-Nitrosothiols and Plasma Nitrates/Nitrites Following Inhalation of Diesel Emissions

Epidemiological studies have associated traffic-related airborne pollution with adverse cardiovascular outcomes. Nitric oxide (NO) is a common component of fresh diesel and gasoline engine emissions that rapidly transforms both in the atmosphere and once inhaled. Because of this rapid transformation, limited information is available in terms of potential human exposures and adverse health effects. Young rats were exposed to whole diesel emissions (DE) adjusted to 300 μg/m3 of particulate matter (containing 3.5 ppm NO) or 0, 3, or 10 ppm NO as a positive control. Animals were also pre-injected (ip) with either saline or N-acetylcysteine (NAC), a precursor of glutathione. Predictably, pure NO exposures led to a concentration-dependent increase in plasma nitrates compared to controls, which lasted for roughly 4 h postexposure. Whole DE exposure for 1 h also led to a doubling of plasma NOx. NAC injection increased the levels of plasma nitrates and nitrites (NOx) in the DE exposure group. Inhibition of nitric oxide symthase (NOS) by N G-nitro-L-arginine (L-NNA) did not block the rise in plasma NOx, demonstrating that the increase was entirely due to exogenous sources. Both DE and pure NO exposures paradoxically led to elevated eNOS expression in aortic tissue. Furthermore, coronary arterioles from NO-exposed animals exhibited greater constriction to endothelin-1 compared to controls, consistent with a derangement of the NOS system. Thus, NO may be an important contributor to traffic-related cardiovascular morbidity, although further research is necessary for proper hazard identification.
A Francisella tularensis pathogenicity island protein essential for bacterial proliferation within the host cell cytosol

Francisella tularensis is an intracellular bacterial pathogen, and is a category A bioterrorism agent. Within quiescent human macrophages, the F. tularensis pathogenicity island (FPI) is essential for bacterial growth within quiescent macrophages. The F. tularensis‐containing phagosome matures to a late endosome‐like stage that does not fuse to lysosomes for 1–8 h, followed by gradual bacterial escape into the macrophage cytosol. Here we show that the FPI protein IglD is essential for intracellular replication in primary human monocyte‐derived macrophages (hMDMs). While the parental strain replicates robustly in pulmonary, hepatic and splenic tissues of BALB/c mice associated with severe immunopathologies, the isogenic iglD mutant is severely defective. Within hMDMs, the iglD mutant‐containing phagosomes mature to either a late endosome‐like phagosome, similar to the parental strain, or to a phagolysosome, similar to phagosomes harbouring the iglC mutant control. Despite heterogeneity and alterations in phagosome biogenesis, the iglD mutant bacteria escape into the cytosol faster than the parental strain within hMDMs and pulmonary cells of BALB/c mice. Co‐infections of hMDMs with the wild‐type strain and the iglD mutant, or super‐infection of iglD mutant‐infected hMDMs with the wild‐type strain show that the mutant strain replicates robustly within the cytosol of hMDMs coinhabited by the wild strain. However, when the wild‐type strain‐infected hMDMs are super‐infected by the iglD mutant, the mutant fails to replicate in the cytosol of communal macrophages. This is the first demonstration of a F. tularensis novel protein essential for proliferation in the macrophage cytosol. Our data indicate that F. tularensis transduces signals to the macrophage cytosol to remodel it into a proliferative niche, and IglD is essential for transduction of these signals.
Physical activity: perceptions of people with severe traumatic brain injury living in the community

ABSTRACT Primary objective: Increasing physical activity (PA) among people with severe traumatic brain injury (TBI) represents an important long-term rehabilitation goal. To design effective interventions to promote PA, the factors associated with PA engagement post-TBI need to be understood. Research design: A qualitative study design was employed to investigate the factors influencing PA engagement in people with severe TBI living in the community. Methods and Procedures: Face-to-face interviews were conducted with eight people with severe TBI three to five years post-injury. A constant comparative method of data collection and analysis was adopted. Main outcomes and results: Interviews were analysed using thematic analysis. Three themes were identified: continuance of self and PA (perception of self, stage of life, and PA normality), beliefs about PA (knowledge of PA and associated benefits), and purpose of PA engagement (reasons for being physically active). Lifelong PA habits and current life priorities impacted on PA engagement post-TBI and influenced whether TBI-associated impairments were considered as a barrier to PA. Conclusion: Among this group of people, PA engagement post-TBI was influenced by perceptions of lifelong PA habits and current life priorities. Interventions to increase PA need to address these perceptions and adapt PA to account for life priorities within the context of TBI-associated impairments.