Miracle-dz/UniFAR-FTUs huggingface dataset:
Human Immunodeficiency Virus Type 1 N-Terminal Capsid Mutants Containing Cores with Abnormally High Levels of Capsid Protein and Virtually No Reverse Transcriptase

ABSTRACT We previously described the phenotype associated with three alanine substitution mutations in conserved residues (Trp23, Phe40, and Asp51) in the N-terminal domain of human immunodeficiency virus type 1 capsid protein (CA). All of the mutants produce noninfectious virions that lack conical cores and, despite having a functional reverse transcriptase (RT), are unable to initiate reverse transcription in vivo. Here, we have focused on elucidating the mechanism by which these CA mutations disrupt virus infectivity. We also report that cyclophilin A packaging is severely reduced in W23A and F40A virions, even though these residues are distant from the cyclophilin A binding loop. To correlate loss of infectivity with a possible defect in an early event preceding reverse transcription, we modeled disassembly by generating viral cores from particles treated with mild nonionic detergent; cores were isolated by sedimentation in sucrose density gradients. In general, fractions containing mutant cores exhibited a normal protein profile. However, there were two striking differences from the wild-type pattern: mutant core fractions displayed a marked deficiency in RT protein and enzymatic activity (<5% of total RT in gradient fractions) and a substantial increase in the retention of CA. The high level of core-associated CA suggests that mutant cores may be unable to undergo proper disassembly. Thus, taken together with the almost complete absence of RT in mutant cores, these findings can account for the failure of the three CA mutants to synthesize viral DNA following virus entry into cells.
Revenu et Mortalité : Analyse Economique des Inégalités Sociales de Santé en France

Cette these propose une analyse empirique de l'influence du revenu et des inegalites de revenu sur la mortalite en France. Pour pallier l'absence d'information sur le revenu dans les donnees demographiques disponibles, cette analyse a ete realisee a partir d'une enquete cas-temoins construite a partir de deux bases de donnees fiscales : l'enquete Patrimoine au Deces en 1988 et l'enquete sur Revenus fiscaux des menages de l'annee 1990. Cette demarche permet d'identifier les determinants de la probabilite de deces en 1988 en comparant les caracteristiques d'un echantillon de defunts a celle d'un echantillon de survivants. Une premiere analyse, realisee a l'aide d'une methode d'estimation indirecte de la mortalite, montre que la fonction de survie est a chaque âge croissante avec le revenu. L'exploitation de l'enquete cas-temoins permet de confirmer l'existence d'une tres forte correlation entre revenu et mortalite, apres controle de la profession. L'effet protecteur du revenu existe a chaque niveau de la distribution des revenus. Les resultats indiquent une surmortalite liee a la pauvrete mais aussi un effet moins attendu, une sous-mortalite liee aux plus hauts revenus. Une analyse multi-niveaux permet de montrer que le niveau des inegalites de revenu de la region d'habitation constitue en lui-meme un determinant de la mortalite apres controle par l'offre de soins. Cette these suggere que le risque de deces depend tres fortement du niveau absolu de ressources de l'individu mais aussi plus largement de son environnement economique et social.
Orexin/hypocretin receptor signalling: a functional perspective

Multiple homeostatic systems are regulated by orexin (hypocretin) peptides and their two known GPCRs. Activation of orexin receptors promotes waking and is essential for expression of normal sleep and waking behaviour, with the sleep disorder narcolepsy resulting from the absence of orexin signalling. Orexin receptors also influence systems regulating appetite/metabolism, stress and reward, and are found in several peripheral tissues. Nevertheless, much remains unknown about the signalling pathways and targets engaged by native receptors. In this review, we integrate knowledge about the orexin receptor signalling capabilities obtained from studies in expression systems and various native cell types (as presented in Kukkonen and Leonard, this issue of British Journal of Pharmacology) with knowledge of orexin signalling in different tissues. The tissues reviewed include the CNS, the gastrointestinal tract, the pituitary gland, pancreas, adrenal gland, adipose tissue and the male reproductive system. We also summarize the findings in different native and recombinant cell lines, especially focusing on the different cascades in CHO cells, which is the most investigated cell line. This reveals that while a substantial gap exists between what is known about orexin receptor signalling and effectors in recombinant systems and native systems, mounting evidence suggests that orexin receptor signalling is more diverse than originally thought. Moreover, rather than being restricted to orexin receptor ‘overexpressing’ cells, this signalling diversity may be utilized by native receptors in a site‐specific manner.
Propagation of sound waves in stressed elasto-plastic material

Acoustoelasticity approach is a method of non-destructive testing and it is based upon the theory of propagation of ultrasonic longitudinal and transverse waves of different polarization in solids. This approach is able to uniquely determine the value of the principal stress in the case of no plastic deformation. An elastoplastic material with hardening is taken and the problem of propagation of a plane acoustic wave in a homogeneous prismatic elastic-plastic body that is uniaxially prestressed in the direction perpendicular to the wave propagation direction is solved. The sound velocities for the transverse and longitudinal waves are obtained for different approximations in terms of the first and second order of smallness. A strong dependence of the velocities from the hardening factor and component of the deviatoric stress tensor was detected. The study provides a number of closed-form expressions required for solving the practical problem of technical diagnostics of structures exposing plasticity.
[Comparative study on abdominal acupuncture and western medicine for treatment of menopause depressive disorder].

OBJECTIVE To observe the therapeutic effects between abdominal acupuncture and Deanxit for treatment of menopause depressive disorder, and to explore the efficacy and safety of abdominal acupuncture. METHODS Sixty cases were randomly divided into an observation group and a control group, 30 cases in each. The observation group was treated with abdominal acupuncture at Zhongwan (CV 12), Xiawan (CV 10), Qihai (CV 6) and Guanyuan (CV 4), etc. The control group was treated with oral administration of Deanxit. The patients in both groups were treated for 4 weeks and followed up for another 4 weeks, and they were evaluated by Hamilton Depression Scale (HAMD) every couple weeks. RESULTS The total HAMD scores of 2 and 4 weeks treatments and 2 and 4 weeks follow-up were all reduced in both groups (all P < 0.01). The total scores of 2 and 4 weeks follow-up in observation group were lower than those in control group, with significant differences in statistical analysis (both P < 0.05). Compared with the clinical therapeutic effect of both groups after 4 weeks treatment, there was no significant difference (P > 0.05), however, after 4 weeks follow-up, the therapeutic effect in observation group was superior to that in control group, with significant difference in statistical analysis (P < 0.05). The safety indexes before and after treatment of both groups were normal, and the adverse reaction rate in observation group was much lower than that in control group (P < 0.05). CONCLUSION Abdominal acupuncture is an effective and safe method for menopause depressive disorder, it improves the menopause depressive symptoms with persistent action, less symptoms relapse and adverse reactions.
Assessment of Arterial Stiffness in Female and Male Gout Patients

The aim of this study was to investigate the relationship between central blood pressures (BP), pulse wave velocity (PWV) meaurements, and biochemical parameters in female and male gout patients and controls. This study included 12 (23.5%) females and 39 (76.5%) males with gout disease. 24-hour diastolic BP, day diastolic BP and day diastolic exceeding limit value were higher in male gout patients than female gout patients. In male gout patients, more prominent inflammatory changes in the vascular wall may be an explanation for the result of this our study, because history of hypertension and smoking habits were frequent in this group.
Ca(2+)-related changes in the mouse sperm capacitation state: a possible role for Ca(2+)-ATPase.

Mammalian spermatozoa require extracellular Ca2+, some of which must be internalized, to undergo complete capacitation. At a critical threshold, a rise in intracellular Ca2+ will trigger acrosomal exocytosis. We used chlortetracycline (CTC) fluorescence patterns to assess changes in the capacitation state of mouse spermatozoa after incubation under various conditions that would affect their intracellular Ca2+ concentrations. Under standard conditions with 1.80 mmol CaCl2l-1 known to support capacitation within 120 min and subsequent fertilization in vitro, a rise in the number of capacitated, acrosome-intact cells (B pattern) was observed over the first 60 min, followed by a decline. A detectable increase in capacitated, acrosome-reacted cells (AR pattern) coincided with the maximum of B pattern cells and a continued rise was observed over the following 60 min. With incubation in 3.60 mmol Ca2+l-1, the rise in AR cells began at 30 min, suggesting that this treatment accelerates capacitation. Introduction of ionophore A23187 at 15 min to cells in standard Ca2+ produced a similar but even more rapid response, with a maximum in B pattern cells and a noticeable rise in AR cells within 10 min. Thus ionophore-treated cells proceed through capacitation, but do so very quickly. However, ionophore in the presence of 90 mumol Ca2+l-1 could promote transition from the uncapacitated F pattern to the capacitated B pattern, but could not trigger acrosomal exocytosis, indicating that the latter requires high extracellular Ca2+. After preincubation in Ca(2+)-deficient medium, most cells exhibited the uncapacitated F pattern and the introduction of millimolar Ca2+ altered this distribution only slowly, over a period of 50 min. In contrast, preincubation in 90 mumol Ca2+l-1 resulted in a minority of F pattern cells and, within 10 min of millimolar Ca2+ introduction, a significant increase in AR cells was observed. (ABSTRACT TRUNCATED AT 250 WORDS)
Nasal high flow nebulization in infants and toddlers: An in vitro and in vivo scintigraphic study

Aerosol therapy in infants and toddlers is challenging. Nebulization within a nasal high flow (NHF) circuit is attractive. The aim of this study was to quantify aerosol lung deposition when combined with NHF as compared with standard practice. Lung doses were measured scintigraphically after nebulization with jet and mesh nebulizer placed within a NHF circuit in a spontaneously breathing non‐human primate model (macaque) and in the anatomical bench SAINT model, respectively representing a full‐term newborn and a 9‐month‐old toddler. In the SAINT model, lung depositions observed with the mesh nebulizer placed in the NHF circuit set at 2 and 4 L/min were 3.3% and 4.2% of the nebulizer charge, respectively, and similar to the 1.70% observed with the control standard facemask jet nebulization (6 L/min flow). In the macaque model, the depositions observed with the mesh nebulizer in the NHF circuit set at 2 and 4 L/min were 0.49% and 0.85%, respectively, also similar to the control measurement (0.71%). Mesh nebulization within a NHF circuit set at 8 L/min and jet nebulization either within a NHF circuit or placed on top of the cannula (NHF set at 2 L/min; total flow of 8 L/min), resulted in a significantly lower lung depositions. Mesh nebulization within a NHF circuit delivering up to 4 L/min gas is likely to be at least as effective than jet nebulization with a facemask in infants and toddlers. Aerosol facemask placement on top of cannulas or jet nebulization within the NHF circuit may be less effective. Pediatr Pulmonol. 2017;52:337–344. © 2016 Wiley Periodicals, Inc.
Limits to sustained energy intake. XXII. Reproductive performance of two selected mouse lines with different thermal conductance

Maximal sustained energy intake (SusEI) appears limited, but the factors imposing the limit are disputed. We studied reproductive performance in two lines of mice selected for high and low food intake (MH and ML, respectively), and known to have large differences in thermal conductance (29% higher in the MH line at 21°C). When these mice raised their natural litters, their metabolisable energy intake significantly increased over the first 13 days of lactation and then reached a plateau. At peak lactation, MH mice assimilated on average 45.3% more energy than ML mice (222.9±7.1 and 153.4±12.5 kJ day−1, N=49 and 24, respectively). Moreover, MH mice exported on average 62.3 kJ day−1 more energy as milk than ML mice (118.9±5.3 and 56.6±5.4 kJ day−1, N=subset of 32 and 21, respectively). The elevated milk production of MH mice enabled them to wean litters (65.2±2.1 g) that were on average 50.2% heavier than litters produced by ML mothers (43.4±3.0 g), and pups that were on average 27.2% heavier (9.9±0.2 and 7.8±0.2 g, respectively). Lactating mice in both lines had significantly longer and heavier guts compared with non-reproductive mice. However, inconsistent with the ‘central limit hypothesis’, the ML mice had significantly longer and heavier intestines than MH mice. An experiment where the mice raised litters of the opposing line demonstrated that lactation performance was not limited by the growth capacity of offspring. Our findings are consistent with the idea that the SusEI at peak lactation is constrained by the capacity of the mothers to dissipate body heat.
Surveillance for High Pathogenicity Avian Influenza Virus in Wild Birds in the Pacific Flyway of the United States, 2006–2007

Abstract In 2006 the U.S. Department of Agriculture, U.S. Department of Interior, and cooperating state fish and wildlife agencies began surveillance for high-pathogenicity avian influenza (HPAI) H5N1 virus in wild birds in the Pacific Flyway of the United States. This surveillance effort was highly integrated in California, Oregon, Washington, Idaho, Nevada, Arizona, Utah, and western Montana, with collection of samples coordinated with state agencies. Sampling focused on live wild birds, hunter-killed waterfowl during state hunting seasons, and wild bird mortality events. Of 20,888 samples collected, 18,139 were from order Anseriformes (waterfowl) and 2010 were from order Charadriiformes (shorebirds), representing the two groups of birds regarded to be the primary reservoirs of avian influenza viruses. Although 83 birds were positive by H5 real-time reverse transcription polymerase chain reaction (rRT-PCR), no HPAI H5N1 virus was found. Thirty-two virus isolates were obtained from the H5-positive samples, including low-pathogenicity H5 viruses identified as H5N2, H5N3, and H5N9.
Animations rendered by braitenberg vehicles

We describe an approach to generating animated sequences of images drawn using stroke-based rendering. Individual strokes are generated as a history of movements of a class of purely reactive computational agents known as Braitenberg Vehicles embedded on a digital canvas. The entire animation represents an aggregate of all the movements of the agents across an entire run. We provide end users with a software tool to produce such animations and allow users to set the sensory capabilities of agents on a low level basis. The agents perform feature detection based on such capabilities. The end users thus interact with the agents with a variety of input images to discover a range of renderable outcomes. Artists inform us that the animations that result from our output provide an engaging visual experience.
Impact of Angiotensin II Receptor Blocker on Plasma Levels of Adiponectin and Advanced Oxidation Protein Products in Peritoneal Dialysis Patients

Background: Oxidative stress and adipocytokines are reported to contribute to the pathogenesis of atherosclerosis. Though the inhibition of the renin-angiotensin system is known to have beneficial effects on atherosclerosis, the exact mechanisms for this remain to be clarified. The aim of this study was to determine the effects of angiotensin II receptor blockers (ARB) on the oxidative stress and adipocytokines in peritoneal dialysis patients. Methods: Candesartan (8 mg/day), an ARB, was administered for 3 months to 8 nondiabetic patients on peritoneal dialysis. Plasma levels of advanced oxidation protein products (AOPP) and adiponectin were measured before and 3 months after candesartan administration, and 3 months after discontinuation. Results: Plasma AOPP level decreased from 377.5 to 305.6 µmol/l (p < 0.05) following the administration of candesartan and returned to 394.6 µmol/l (p < 0.05) by 3 months after the discontinuation of the drug. Plasma adiponectin level increased from 12.5 to 18.8 µg/ml (p < 0.05) by candesartan and decreased again to 14.4 µg/ml (p < 0.05) after discontinuation. There was a significant inverse relationship between changes in plasma level of adiponectin and AOPP (r = – 0.888, p < 0.01). Conclusion: Candesartan increases plasma adiponectin level in association with the reduction of oxidative stress in peritoneal dialysis patients. Candesartan may be useful in preventing atherosclerosis in peritoneal dialysis patients.
Analysis of Genetic Diversity of Streptococcus suis Clinical Isolates from Pigs in Spain by Pulsed-Field Gel Electrophoresis

ABSTRACT Pulsed-field gel electrophoresis (PFGE) was used to investigate the diversity of Streptococcus suis isolates of various serotypes recovered from swine clinical samples in Spain. Capsular types 9 (64.9%) and 2 (14.8%) were the most frequently isolated serotypes followed by serotype 7 (5.9%) and serotype 8 (4.3%). The PFGE results of this study with 60 different pulsotypes indicate a great genetic diversity among the S. suis isolates, which is consistent with the broad distribution of S. suis in the swine population. Forty-five percent of the pulsotypes corresponded to single isolates, no pulsotype was common to all farms, and at least 3 different pulsotypes were isolated in 56% of herds in which more than 3 clinical isolates were analyzed. These results reveal a great diversity both between and within herds throughout the strains of S. suis studied, demonstrating that different strains of S. suis are associated with infection in pigs. Some pulsotypes were more frequently isolated and exhibited a wider distribution over herds than others, and were the unique or predominant strains in several herds, suggesting the existence of a prevalent or a few prevalent clones responsible for a large proportion of clinical cases. Overall, the great genetic heterogeneity of the clinical strains of S. suis, the isolation of different strains within the same herd, and the predominance of particular strains in some herds are evidence that infection by S. suis is a dynamic process and reinforce the idea that the epidemiology of S. suis infection is very complex.
A Balanced Tissue Composition Reveals New Metabolic and Gene Expression Markers in Prostate Cancer

Molecular analysis of patient tissue samples is essential to characterize the in vivo variability in human cancers which are not accessible in cell-lines or animal models. This applies particularly to studies of tumor metabolism. The challenge is, however, the complex mixture of various tissue types within each sample, such as benign epithelium, stroma and cancer tissue, which can introduce systematic biases when cancers are compared to normal samples. In this study we apply a simple strategy to remove such biases using sample selections where the average content of stroma tissue is balanced between the sample groups. The strategy is applied to a prostate cancer patient cohort where data from MR spectroscopy and gene expression have been collected from and integrated on the exact same tissue samples. We reveal in vivo changes in cancer-relevant metabolic pathways which are otherwise hidden in the data due to tissue confounding. In particular, lowered levels of putrescine are connected to increased expression of SRM, reduced levels of citrate are attributed to upregulation of genes promoting fatty acid synthesis, and increased succinate levels coincide with reduced expression of SUCLA2 and SDHD. In addition, the strategy also highlights important metabolic differences between the stroma, epithelium and prostate cancer. These results show that important in vivo metabolic features of cancer can be revealed from patient data only if the heterogeneous tissue composition is properly accounted for in the analysis.
An object detector based on multiscale sliding window search using a fully pipelined binarized CNN on an FPGA

An object detection problem consists of two problems: one is classification of detected object category and the other is localization. Frame object detection is used in an embedded vision systems, such as a robot, an automobile, a security camera, and a drone. These applications require high-performance computation and low-power consumption by an inexpensive device. This paper proposes multiscale sliding window based object detector using a fully pipelined binarized deep convolutional neural network (BCNN) on an FPGA. It consists of a sliding window part, a fully pipelined BCNN classifier, and an ARM processing unit for detection. Duplicate detections were filtered by using a non-maximum suppression algorithm running on the ARM processor. We propose the fully pipelined layers for the BCNN and its architecture for FPGA realization. Since the proposed BCNN circuit uses on-chip memories on the FPGA, its throughput is higher than a GPU based one with practical recognition accuracy. We trained the VGG11 based BCNN using the KITTI vision benchmark for the car detection scenario. Then, we implemented the proposed object detector on the Xilinx Inc. Zynq UltraScale+ MPSoC zcu102 evaluation board. The GPU based object detectors were too slow for the realtime application requirement (HD frame rate), with the exception of YOLOv2. As compared with the GPU implementation of YOLOv2, the proposed FPGA detector had higher recognition accuracy and lower power consumption. Compared with the YOLOv2, the proposed FPGA one is higher with respect to recognition accuracy, and its power consumption is lower than the GPU based YOLOv2. Thus, the FPGA based object detector suitable for the embedded realtime applications.
Investigations in Stellar Spectroscopy: IV. Spectroscopic Evidence for the Existence of Two Classes of M Type Stars.

appear that a relationship between certain spectral characteristics and the distances of stars could hardly be credible, since it would appear like a correlation between two utterly unrelated subjects except in so far as the scattering of light in space might connect them. In fact, of course, it is not the distances but the absolute magnitudes of stars which have an influence on the character of the spectrum lines and such an effect, far from being improbable, is rather to be expected than not. The derivation of the distances is merely a by-product resulting from the combination of real, or absolute, with apparent magnitudes. An important gain in the value of this method of determining stellar magnitudes and distances should result from an increase in the number of measured parallaxes of bright stars of small proper motion. Such stars will on the average prove to be very luminous, and, as already stated, the portion of the curves connecting line intensity with absolute magnitude is subject to much more uncertainty in the case of the high luminosity stars than in any of the others. It is probable that after such a revision has been made the method will find its most important application as a means of distinguishing these giant stars in the stellar