Miracle-dz/UniFAR-FTUs huggingface dataset:
Temporal variability of bacterial communities in cryoconite on an alpine glacier

Cryoconite holes, that is, small ponds that form on glacier surface, are considered the most biologically active environments on glaciers. Bacterial communities in these environments have been extensively studied, but often through snapshot studies based on the assumption of a general stability of community structure. In this study, the temporal variation of bacterial communities in cryoconite holes on the Forni Glacier (Italian Alps) was investigated by high throughput DNA sequencing. A temporal change of bacterial communities was observed with autotrophic Cyanobacteria populations dominating communities after snowmelt, and heterotrophic Sphingobacteriales populations increasing in abundance later in the season. Bacterial communities also varied according to hole depth and area, amount of organic matter in the cryoconite and oxygen concentration. However, variation in environmental features explained a lower fraction of the variation in bacterial communities than temporal variation. Temporal change along ablation season seems therefore more important than local environmental conditions in shaping bacterial communities of cryoconite of the Forni Glacier. These findings challenge the assumption that bacterial communities of cryoconite holes are stable.
Muscarinic stimulation of tracheal smooth muscle cells activates large-conductance Ca(2+)-dependent K+ channel.

We investigated the regulation of the large-conductance Ca(2+)-dependent K+ (KCa) channel by acetylcholine (ACh) in freshly dissociated tracheal smooth muscle cells. Channels were recorded in the cell-attached patch configuration, and cells were stimulated with ACh, muscarine, or caffeine. We identified KCa channel activity based on 1) the voltage dependence of channel opening; 2) the large unitary conductance (242 +/- 5 pS with symmetrical 135 mM K+); 3) dependence of the reversal potential on the [K+] gradient, shifting 56 +/- 3 mV/10-fold change in extracellular [K+]; and 4) opening of channels after elevation of cytosolic free Ca2+ concentration ([Ca2+]i) using the Ca2+ ionophore A23187. When cells were bathed either in a physiological saline solution or a solution containing 135 mM K+ (to clamp cell membrane potential near 0 mV), ACh caused contraction of cells and activation of voltage-dependent channels. With 135 mM extracellular K+, the channels activated by ACh had a unitary conductance of 247 +/- 10 pS, and currents reversed near the K+ equilibrium potential (-1 +/- 1 mV). The effects of ACh were reversible, blocked by atropine, and mimicked by muscarine. From these characteristics we conclude that muscarinic stimulation of canine tracheal smooth muscle cells leads to activation of the large-conductance KCa channel. Because the KCa channels were isolated from ACh by the patch pipette, the increased channel activity was probably mediated by a cytosolic second messenger. ACh shifted the threshold for KCa channel opening to less positive membrane potentials, similar to that seen with elevation of [Ca2+]i. (ABSTRACT TRUNCATED AT 250 WORDS)
GLOBAL EMISSIONS: A NEW CONTRIBUTION FROM THE SHADOW ECONOMY

Based on the STIRPAT model and the EKC hypothesis, this study provides new evidences on the economic determinants of global emissions. The system-GMM estimations are used for the sample of 106 economies in the period of 1995-2012 to investigate the influences of income level, urbanization, industrialization, energy intensity, public expenditure, trade openness, FDI inflow, and especially shadow economy on total greenhouse emissions, CO2 emissions, CH4 emissions, and N2O emissions, respectively. This study contributes to the literature in three folds. First, the industrialization energy intensity are the main drivers for all emissions (excluding N2O). While, urbanization has positive effects on emissions excluding the case of CH4. Other drivers including public spending and economic integration (proxied by trade openness and FDI inflow) are also tested with interesting findings. Second, a higher level of shadow economy increases all emissions excluding CO2. Third, the determinants of emissions vary depending on the countries’ income level. The study is supported by a battery of robustness checks and by various estimations in the short and long-run to identify the importance of emissions’ drivers.
Happy parents’ tweets: An exploration of Italian Twitter data using sentiment analysis

Background: Demographers are increasingly interested in connecting demographic behaviour and trends with 'soft' measures, i.e., complementary information on attitudes, values, feelings, and intentions. Objective: The aim of this paper is to demonstrate how computational linguistic techniques can be used to explore opinions and semantic orientations related to parenthood. Methods: In this article we scrutinize about three million ﬁltered Italian tweets from 2014. First, we implement a methodological framework relying on Natural Language Processing techniques for text analysis, which is used to extract sentiments. We then run a supervised machine-learning experiment on the overall dataset, based on the annotated set of tweets from the previous stage. Consequently, we infer to what extent social media users report negative or positive affect on topics relevant to the fertility domain. Results: Parents express a generally positive attitude towards being and becoming parents, but they are also fearful, surprised, and sad. They also have quite negative sentiments about their children’s future, politics, fertility, and parental behaviour. By exploiting geographical information from tweets we find a significant correlation between the prevalence of positive sentiments about parenthood and macro-regional indicators of both life satisfaction and fertility level. Contribution: We show how tweets can be used to represent soft measures such as attitudes, values, and feelings, and we establish how they relate to demographic features. Linguistic analysis of social media data provides a middle ground between qualitative studies and more standard quantitative approaches.
A modified sequential Monte Carlo procedure for the efficient recursive estimation of extreme quantiles

Many applications in science involve finding estimates of unobserved variables from observed data, by combining model predictions with observations. The sequential Monte Carlo (SMC) is a well‐established technique for estimating the distribution of unobserved variables that are conditional on current observations. While the SMC is very successful at estimating the first central moments, estimating the extreme quantiles of a distribution via the current SMC methods is computationally very expensive. The purpose of this paper is to develop a new framework using probability distortion. We use an SMC with distorted weights in order to make computationally efficient inferences about tail probabilities of future interest rates using the Cox–Ingersoll–Ross (CIR) model, as well as with an observed yield curve. We show that the proposed method yields acceptable estimates about tail quantiles at a fraction of the computational cost of the full Monte Carlo.
Macrolevel stressors, terrorism, and mental health outcomes: broadening the stress paradigm.

OBJECTIVES We examined the extent to which the stress paradigm linking psychosocial stressors to mental health status has focused disproportionate attention on microlevel social stressors to the detriment of macrolevel stressors. Also, we assessed the effects of the terrorist attacks of September 11, 2001, on subsequent mental health among participants in a Midwestern cohort study. METHODS Respondents in a 6-wave longitudinal mail survey completed questionnaires before September 11, 2001, and again in 2003 and 2005. Regression analyses focused on measures of negative terrorism-related beliefs and fears, as well as psychological distress and deleterious alcohol use outcomes measured both before and after September 11. RESULTS Negative terrorism-related beliefs and fears assessed in 2003 predicted distress and drinking outcomes in 2005 after control for sociodemographic characteristics and pre-September 11 distress and drinking. CONCLUSIONS The events of September 11 continue to negatively affect the mental health of the American population. Our results support the utility of according greater attention to the effects of such macrolevel social stressors in population studies embracing the stress paradigm.
The Effects of Spatial and Temporal Factors on the Perception of Stroboscopic Rotation Simulations

In the present two experiments subjects viewed discontinuous, i.e. stroboscopic, simulations of a transparent sphere partially filled with randomly positioned luminous dots and rotating about the y axis in depth. Over a range of stimulus conditions, such simulations elicited coherent sensations of continuous rotation and internal volume of the sphere. By manipulating both spatial and temporal variables in the simulations, it was attempted to define the boundary conditions for which corresponding elements of a simulation are perceptually paired from frame to frame to yield coherent sensations of rotation and depth. The results indicated that the process that matches or pairs corresponding elements in a three-dimensional simulation cannot be identified with the process that matches corresponding elements in similar two-dimensional displays. Furthermore, temporal factors influenced the perception of these simulations more than did spatial factors, and rotation-judgment accuracy and perceived depth were different functions of temporal frequency. Over a range of temporal frequencies, such three-dimensional simulations are apparently processed in a manner similar to that in which objects undergoing real motion are processed.
Factors Affecting Results of Ulnar Shortening for Ulnar Impaction Syndrome

Although ulnar shortening osteotomy is the most frequently performed operation for ulnar impaction syndrome, little attention has been given to detect certain preoperative factors affecting clinical outcomes of this procedure. We asked whether preoperative factors influenced the postoperative score of ulnar shortening osteotomy combined with arthro-scopic débridement of the triangular fibrocartilage complex. We retrospectively reviewed 51 patients (53 wrists) with ulnar impaction syndrome treated with this procedure. There were 28 males and 23 females ranging in age from 14 to 67 years (mean, 37.5 years). The minimum followup was 12 months (mean, 26.3 months; range, 12-95 months). At last followup, we determined a modified Mayo wrist score for each patient. Preoperative factors affecting the clinical score were identified using multiple regression analysis. The clinical score ranged from 40 to 100 points (mean, 84.5 points). A long duration of symptoms and workers' compensation predicted worse clinical scores. We recommend considering these two factors when deciding whether to perform this procedure.Level of Evidence: Level IV, therapeutic study. See the Guidelines for Authors for a complete description of levels of evidence.
Infanticide secrets: qualitative study on postpartum depression.

OBJECTIVE To explore thoughts of infanticide that did not lead to the act among mothers with postpartum depression. DESIGN A phenomenologic hermeneutic study in which women were invited to share their experiences of having thoughts of infanticide. SETTING Community setting in a large metropolitan city, Brisbane, Australia. PARTICIPANTS Fifteen women who had been diagnosed as clinically depressed with postpartum onset whose babies were 12 months of age or younger. METHOD Audiotaped, in-depth interviews were transcribed verbatim. Thematic analysis commenced immediately after the first interview, and data collection continued until saturation was achieved. A questioning approach that reflected hermeneutics was facilitated by use of journals by the researchers. MAIN FINDINGS Six themes emerged from the data: imagined acts of infanticide, the experience of horror, distorted sense of responsibility, consuming negativity, keeping secrets, and managing the crisis. CONCLUSION Women who experienced nonpsychotic depression preferred not to disclose their thoughts of infanticide to health professionals, including trusted general practitioners or psychiatrists. These women were more likely to mention their suicidal thoughts than their infanticidal thoughts in order to obtain health care. General practitioners and other health professionals should directly ask about whether a woman has been experiencing thoughts of harming herself or her baby, regardless of the reason why she has presented.
Financial Liberalisation and Interest Rate Determination in Malaysia

The paper investigates the importance of external factors in the domestic interest rate determination process and measures the openness and adjustment speed of Malaysian financial market. It also seeks to capture the effects of the Asian Financial Crisis 1997 (AFC) and the Malaysian government approaches to the crisis onto the process. Ordinary Least Square estimation method is employed on the time series data of domestic interest rates, foreign and domestic variables. Results of this study indicate the external factors are dominant in the domestic interest rate determination pre-AFC, but post-AFC domestic variables appear to affect most. In addition, the paper found that Malaysian domestic market is less opened with limited speed of foreign adjustment post-AFC.
A Framework and Taxonomy of Videogame Playing Preferences

Player preferences for different gaming styles or game elements has been a topic of interest in human-computer interaction for over a decade. However, current models suggested by the extant literature are generally based on classifying abstract gaming motivations or player archetypes. These concepts do not directly map onto the building blocks of games, taking away from the utility of the findings. To address this issue, we propose a conceptual framework of player preferences based on two dimensions: game elements and game playing styles. To investigate these two concepts, we conducted an exploratory empirical investigation of player preferences, which allowed us to create a taxonomy of nine groups of game elements and five groups of game playing styles. These two concepts are foundational to games, which means that our model can be used by designers to create games that are tailored to their target audience. In addition, we demonstrate that there are significant effects of gender and age on participants' preferences and discuss the implications of these findings.
The SALTER Model of the World Economy: Model Structure, Database and Parameters

The SALTER Model of the world Economy: Model Structure, Database and Parameters by Jornini, P., McDougall, R. , Watts, G. and Dee, P.S. , is Working Paper number 24 in the development of The SALTER Model of the World Economy. Available for download at: http://www.pc. gov.au/research/supporting/salter-model/workingpaper24
Multiparameter flow cytometry of bacteria: implications for diagnostics and therapeutics.

BACKGROUND Flow cytometric studies of antibiotic susceptibilities of bacteria have typically measured a single fluorescence parameter, such as membrane potential (indicating viability), or permeability to nucleic acid stains such as propidium (indicating nonviability). Cytometry of bacteria stained simultaneously with a membrane potential dye and a permeability indicator reveals unanticipated complexity. METHODS Aliquots of cultures of three bacterial species were stained with the potential-sensitive dye hexamethylindocarbocyanine [DiIC1(3)] and the permeability indicator TO-PRO-3, in the presence and absence of a proton ionophore which collapses the potential gradient. They were analyzed using a dual-laser flow cytometer. RESULTS Cultures grown under suboptimal conditions appear to contain cells that take up TO-PRO-3 while maintaining membrane potential, although some events showing both high DiIC1(3) fluorescence and high TO-PRO-3 fluorescence may represent clumps. CONCLUSIONS Variations in metabolic patterns between species and within organisms under suboptimal culture conditions or following antibiotic exposure may make it difficult to develop flow cytometric clinical assays for antibiotic susceptibility. However, transient permeabilization of otherwise resistant organisms by sublethal doses of antibiotics may make it possible to treat infections by such organisms with suitably derivatized, otherwise toxic molecules; multiparameter cytometry should be useful in pursuing this approach to therapy.
CEO/CFO Relevance and Corporate Earnings Management

It’s a fact that the CEO-CFO connection will be tighter if they have appointment relationships, which may lead to the disfunction of CFOs in regulating and supervising corporate financial activities. In order to explore the actual influence of CEO-CFO connections, we studied the correlation between CEO-CFO connectedness and the degree of corporate earnings management using the China listed company data from year 2005 to 2014. Results showed that CEO-CFO connections will increase corporate earnings management after considering the year fixed effect and the industry fixed effect. We control for corporate operation, CEO background and corporate governance factors. Further regressions are done using grouped samples divided by company size and ownership structure. The findings remain solid even after considering of numbers of robustness tests. Besides, we find that CEO duality and equity incentive will enhance the positive relationship.
Vascular Endothelial Growth Factor Receptor KDR Tyrosine Kinase Activity Is Increased by Autophosphorylation of Two Activation Loop Tyrosine Residues*

Vascular endothelial growth factor is an important physiological regulator of angiogenesis. The function of this endothelial cell selective growth factor is mediated by two homologous tyrosine kinase receptors, fms-like tyrosine kinase 1 (Flt-1) and kinase domain receptor (KDR). Although the functional consequence of vascular endothelial growth factor binding to the Flt-1 receptor is not fully understood, it is well established that mitogenic signaling is mediated by KDR. Upon sequencing several independent cDNA clones spanning the cytoplasmic region of human KDR, we identified and confirmed the identity of a functionally required valine at position 848 in the ATP binding site, rather than the previously reported glutamic acid residue, which corresponds to an inactive tyrosine kinase. The cytoplasmic domain of recombinant native KDR, expressed as a glutathione S-transferase fusion protein, can undergo autophosphorylation in the presence of ATP. In addition, the kinase activity can be substantially increased by autophosphorylation at physiologic ATP concentrations. Mutation analysis indicates that both tyrosine residues 1054 and 1059 are required for activation, which is a consequence of an increased affinity for both ATP and the peptide substrate and has no effect on k cat, the intrinsic catalytic activity of the enzyme. KDR kinase catalyzes phosphotransfer by formation of a ternary complex with ATP and the peptide substrate. We demonstrate that tyrosine kinase antagonists can preferentially inhibit either the unactivated or activated form of the enzyme.
Is Cleft Severity Related to Maxillary Growth in Patients with Unilateral Cleft Lip and Palate?

Objective To study the relationship of cleft severity and maxillary growth in patients with unilateral cleft lip and palate. Design A systematic literature review. Methods A literature survey from the PubMed database from January 1966 to December 2008 used the Medical Subject Headings terms facial growth, unilateral cleft lip palate, cephalometry, and cleft severity or cleft width. The Cleft Palate–Craniofacial Journal from 1964 to November 2008 was hand searched. Studies published as full-length articles reporting quantitative data on the relationship of cleft severity and written in English were selected. Two reviewers selected and extracted the data independently and also assessed the quality of the studies. Results Four studies met the selection criteria and were included in the review. All were retrospective and longitudinal. Quality analysis revealed medium to low level of the included studies. Heterogeneity of the studies prevented major conclusions about the relationship of a severe cleft and unfavorable maxillary forward growth. Conclusions The review has highlighted the importance of further research. Further well-designed controlled studies and long-term studies are needed, and researchers have to consider combined assessment of cleft size and palate size. Further studies should also focus on the cleft severity at birth and at the time of palate repair.
A cross‐sectional study on clinical correlates of anxiety disorders in 613 community living older adults in Hong Kong

Previous studies have reported that the prevalence of late life anxiety disorders varies between 3.2% and 15.3%. Despite its high prevalence, anxiety disorders are still under‐recognized in old age. This study evaluated the association among health, psychosocial factors, lifestyle choices, cognitive function and anxiety disorders in the older population in Hong Kong.
Molecular Cloning, Characterization, and Dynamics of Rat Formiminotransferase Cyclodeaminase, a Golgi-associated 58-kDa Protein*

A peripherally associated 58-kDa Golgi protein (58K) of unknown function has been previously described (Bloom, G. S., and Brashear, T. A. (1989) J. Biol. Chem.264, 16083–16092). To molecularly characterize 58K, we used a monoclonal anti-58K antibody (monoclonal antibody 58K-9) to screen a rat liver cDNA expression library. Positive clones were isolated, characterized, and partially sequenced. The obtained sequences show a high level of identity with sequences of porcine formiminotransferase cyclodeaminase (FTCD), suggesting that 58K is rat FTCD. Rat FTCD is structurally similar to porcine FTCD, a metabolic enzyme involved in conversion of histidine to glutamic acid, and exists in dimeric, tetrameric, and octameric complexes resistant to proteolysis. To define parameters of FTCD association with the Golgi, comparison of its behavior with various Golgi and ER-to-Golgi intermediate compartment marker proteins was examined under specific conditions. The results show that extraction parameters of FTCD are similar to those of GM130, a tightly associated Golgi matrix protein. FTCD appears to be a dynamic component of the Golgi, and a proportion of FTCD molecules cycle between the Golgi and earlier compartments of the secretory pathway. FTCD remains associated with Golgi fragments during microtubule disruption and is not released into cytosol during brefeldin A treatment. Instead, FTCD relocates from the Golgi, but the time course of its redistribution is distinct from that of mannosidase II relocation. FTCD is already dispersed into small punctate structures at a time when mannosidase II is still largely localized to Golgi structures. FTCD is not observed in tubules originating from the Golgi and containing mannosidase II. Instead, it appears to redistribute in small vesicles arranged in a linear “pearls on a string” pattern. These results suggest that FTCD relocation is temporally and spatially distinct from mannosidase II relocation and that FTCD provides a novel marker to study Golgi dynamics.