Miracle-dz/UniFAR-FTUs huggingface dataset:
A fusion product of the complete Borrelia burgdorferi outer surface protein A (OspA) and the hepatitis B virus capsid protein is highly immunogenic and induces protective immunity similar to that seen with an effective lipidated OspA vaccine formula

The immunogenicity of peptides and protein fragments can be considerably enhanced by their presentation on particulate carriers such as capsid‐like particles (CLP) from hepatitis B virus (HBV). Here we tested the suitability of the HBV capsid protein as a carrier for a relevant full‐length pathogen‐derived protein antigen. The entire 255‐amino acid ectodomain of the outer surface protein A (OspA) from Borrelia burgdorferi, the causative agent of Lyme disease, was inserted into the major B cell epitope of the HBV capsid, yielding a multimerization‐competent fusion protein, termed coreOspA. CoreOspA, consisting only in part of regular CLP, induced antibodies to OspA, including the Ig isotype profile and specificity for the protective epitope LA‐2, with an efficiency similar to that of recombinant lipidated OspA, the first generation vaccine against Lyme disease. Moreover, coreOspA actively and passively protected mice against subsequent challenge with B. burgdorferi. The data demonstrate the capacity of the HBV capsid protein to act as a potent immunomodulator even for full‐length and structurally complex polypeptide chains and thus opens new avenues for novel vaccine designs.
Snaking of Multiple Homoclinic Orbits in Reversible Systems

We study N-homoclinic orbits near a heteroclinic cycle in a reversible system. The cycle is assumed to connect two equilibria of saddle-focus type. Using Lin's method, we establish the existence of infinitely many N-homoclinic orbits for each N near the cycle. In particular, these orbits exist along snaking curves, thus mirroring the behavior of 1-homoclinic orbits. The general analysis is illustrated by numerical studies for a Swift–Hohenberg system.
Cops on Film: Hollywood’s Depiction of Law Enforcement in Popular Films, 1984–2014

ABSTRACT With numerous recent incidents in which law-enforcement officers played a role in the deaths of citizens, there is a renewed focus on cops and their actions. Part of that discussion is related to the nation’s preconceived notions of cops and where those ideas might originate. Popular culture contributes to those images; this study explores one source of those images: film. More specifically, it investigates the image of law enforcement on the silver screen from 1984 through 2014. With a sample of 34 films and more than 200 cop characters, this study finds a mixed general depiction of law enforcement in movies but a positive depiction of individual cop characters. The prevalent descriptor of those characters was good, hard-working, and competent law-enforcement officers. This exploratory study informs broader discussions about the images of cops found in popular culture.
Changing Livelihoods in Rural Cambodia: Evidence from Panel Household Data in Stung Treng

Analysis of livelihood strategies can aid to understand and resolve problems associated with vulnerability to poverty and food security. This paper aims to identify and describe the changes in rural livelihood activities by using household data for 2013 and 2014 collected in Stung Treng, Cambodia. We use the same variables and estimate different clusters for both the years. The paper concludes that despite the lag of only one year, there are noticeable changes in livelihood strategies. Firstly, we find a group of transition farmers in 2014 that is composed of households that are witnessing a shift towards commercialization. They invest and consume more than subsistence farmers. Secondly, there is a greater diversification in activities amongst the groups. Most households practice multiple activities. Lastly, with regards to self-employment, there has been a shift from agriculture and the production sector to services and crafts. All of the above changes can be deemed as positive as there is a gradual movement away from more vulnerable sectors. Accordingly, households that participate in livelihood activities related to agriculture and natural resource extraction are most affected by shocks and face the highest vulnerability to poverty. The paper additionally highlights some concerns such as a decline in availability of extracted products such as the fish stock which are expected to negatively impact on these more vulnerable rural households in the medium and longer term. Furthermore, the state of education is dismal and needs attention. Therefore, policy makers need to consider these issues while addressing rural poverty.
The Effects of Bench Press Variations in Competitive Athletes on Muscle Activity and Performance

Abstract The aim of the study was to compare the EMG activity performing 6RM competition style bench press (flat bench-wide grip) with 1) medium and narrow grip widths on a flat bench and 1) inclined and declined bench positions with a wide grip. Twelve bench press athletes competing at national and international level participated in the study. EMG activity was measured in the pectoralis major, anterior and posterior deltoid, biceps brachii, triceps brachii and latissimus dorsi. Non-significant differences in activation were observed between the three bench positions with the exception of 58.5-62.6% lower triceps brachii activation, but 48.3-68.7% greater biceps brachii activation in the inclined bench compared with the flat and declined bench position. Comparing the three grip widths, non-significant differences in activations were observed, with the exception of 25.9-30.5% lower EMG activity in the biceps brachii using a narrow grip, compared to the medium and wide grip conditions. The 6-RM loads were 5.8-11.1% greater using a medium and wide grip compared to narrow grip width and 18.5-21.5% lower in the inclined bench position compared with flat and declined. Comparing the EMG activity during the competition bench press style with either the inclined and declined bench position (wide grip) or using a narrow and medium grip (flat bench), only resulted in different EMG activity in the biceps- and triceps brachii. The 6RM loads varied with each bench press variation and we recommend the use of a wide grip on a flat bench during high load hypertrophy training to bench press athletes.
Ultrastructural features of the adult hermaphrodite gonad of Caenorhabditis elegans: relations between the germ line and soma.

Genetic and embryological experiments have established the Caenorhabditis elegans adult hermaphrodite gonad as a paradigm for studying the control of germline development and the role of soma-germline interactions. We describe ultrastructural features relating to essential germline events and the soma-germline interactions upon which they depend, as revealed by electron and fluorescence microscopy. Gap junctions were observed between oocytes and proximal gonadal sheath cells that contract to ovulate the oocyte. These gap junctions must be evanescent since individual oocytes lose contact with sheath cells when they are ovulated. In addition, proximal sheath cells are coupled to each other by gap junctions. Within proximal sheath cells, actin/myosin bundles are anchored to the plasma membrane at plaque-like structures we have termed hemi-adherens junctions, which in turn are closely associated with the gonadal basal lamina. Gap junctions and hemi-adherens junctions are likely to function in the coordinated series of contractions required to ovulate the mature oocyte. Proximal sheath cells are fenestrated with multiple small pores forming conduits from the gonadal basal lamina to the surface of the oocyte, passing through the sheath cell. In most instances where pores occur, extracellular yolk particles penetrate the gonadal basal lamina to directly touch the underlying oocytes. Membrane-bounded yolk granules were generally not found in the sheath cytoplasm by either electron microscopy or fluorescence microscopy. Electron microscopic immunocytochemistry was used to confirm and characterize the appearance of yolk protein in cytoplasmic organelles within the oocyte and in free particles in the pseudocoelom. The primary route of yolk transport apparently proceeds from the intestine into the pseudocoelom, then through sheath pores to the surface of the oocyte, where endocytosis occurs. Scanning electron microscopy was used to directly visualize the distal tip cell which extends tentacle-like processes that directly contact distal germ cells. These distal tip cell processes are likely to play a critical role in promoting germline mitosis. Scanning electron microscopy also revealed thin filopodia extending from the distal sheath cells. Distal sheath filopodia were also visualized using a green fluorescent protein reporter gene fusion and confocal microscopy. Distal sheath filopodia may function to stretch the sheath over the distal arm.
The Effect of Removing Superior Temporal Cortical Motion Areas in the Macaque Monkey: I. Motion Discrimination Using Simple Dots

We compared the pre‐ and postoperative performance of macaque monkeys on visual discrimination tasks entailing the perception of differences in the motion of two luminous spots. Animals in which area MT and adjacent regions had been surgically removed were not significantly impaired as long as there were differences in the temporal frequency of the two stimuli. When the latter were eliminated the postoperative performance of the MT animals was significantly impaired compared to their preoperative performance and compared to the animals in the control groups. The same animals were also impaired at perceiving which of two moving dots, presented in the dark, changed its direction.
A primary full-integrated active filter auxiliary power module in electrified vehicle applications with single-phase onboard chargers

The active filter auxiliary power module (AFAPM) has been proposed for electrified vehicle applications with single-phase onboard chargers. It has two modes: 1) the high-voltage (HV) active filtering mode, in which the vehicle is connected to the grid and the converter assimilates the significant second-order harmonic current introduced by the single-phase power; 2) the low-voltage (LV) battery charging mode, in which the vehicle is running and the converter charges the LV battery from HV battery. This yields a significant capacitance reduction on the DC-link of HV battery charger without an additional active filter (AF) circuit. However, extra relay and inductors are needed. This paper proposes a primary full-integrated AFAPM, which is composed of a two-phase buck converter to work as an AF and a dual-active-bridge (DAB) to operate as a LV battery charger auxiliary power module (APM). With the proposed converter, only an active energy storage capacitor is needed to achieve the active filtering and store the second-order harmonic energy. All the switches and inductors on the primary stage are shared between the AF and APM. Therefore, the cost and size of the dual-voltage charging system in the vehicle applications can be reduced further. To confirm the effectiveness of the proposed converter, a 720 W prototype has been built and experimental results are presented.
Reconstruction of Global Value Chain and Adjustment of China's Export Trade Structure

This paper inspects the form and the driving force of the reconstruction of the global value chain, sets up an industry equilibrium model of the reconstruction to examine the trade concentration of the world's main manufacturing products and analyzes the influence of China as a large trade country in the reconstruction. In view of the fluctuations in the value of China's main export commodities and the proportion and ranking of the value of China's manufacturing export of varied sectors in the world, this paper further studies the impact of the reconstruction on the structure change of China's manufacturing export. The study results show that the reconstruction of the global value chain is currently not prevalent in China, but the trend of the reconstruction is certain. Products that are most likely to face the reconstruction are mechanical or electrical products and high-tech products instead of the traditional labor-intensive products.
Dissociable Neural Mechanisms for Encoding Predictable and Unpredictable Events

Attention is a necessary condition for the formation of new episodic memories, yet little is known about how dissociable attentional mechanisms for top-down and bottom-up orienting contribute to encoding. Here, subjects performed an intentional encoding task in which to-be-learned items were interspersed with irrelevant stimuli such that subjects could anticipate the appearance of some study items but not others. Subjects were more likely to later remember stimuli whose appearance was predictable at encoding. Electroencephalographic data were acquired during the study phase of the experiment to assess how synchronous neural activity related to later memory for predictable stimuli (to which attention could be oriented in a top-down fashion) and unpredictable stimuli (which rely to a greater extent on bottom-up attentional orienting). Over left frontal regions, gamma-band activity (2555 Hz) early (150 msec) in the epoch was a robust predictor of later memory for predictable items, consistent with an emerging view that links high-frequency neural synchrony to top-down attention. By contrast, later (400 msec) theta-band activity (48 Hz) over the left and midline frontal cortex predicted subsequent memory for unpredictable items, suggesting a role in bottom-up attentional orienting. These results reveal for the first time the contribution of dissociable attentional mechanisms to successful encoding and contribute to a growing literature dedicated to understanding the role of neural synchrony in cognition.
Fetal QRS detection and heart rate estimation: a wavelet-based approach

Fetal heart rate monitoring is used for pregnancy surveillance in obstetric units all over the world but in spite of recent advances in analysis methods, there are still inherent technical limitations that bound its contribution to the improvement of perinatal indicators. In this work, a previously published wavelet transform based QRS detector, validated over standard electrocardiogram (ECG) databases, is adapted to fetal QRS detection over abdominal fetal ECG. Maternal ECG waves were first located using the original detector and afterwards a version with parameters adapted for fetal physiology was applied to detect fetal QRS, excluding signal singularities associated with maternal heartbeats. Single lead (SL) based marks were combined in a single annotator with post processing rules (SLR) from which fetal RR and fetal heart rate (FHR) measures can be computed. Data from PhysioNet with reference fetal QRS locations was considered for validation, with SLR outperforming SL including ICA based detections. The error in estimated FHR using SLR was lower than 20 bpm for more than 80% of the processed files. The median error in 1 min based FHR estimation was 0.13 bpm, with a correlation between reference and estimated FHR of 0.48, which increased to 0.73 when considering only records for which estimated FHR > 110 bpm. This allows us to conclude that the proposed methodology is able to provide a clinically useful estimation of the FHR.
The role of decentralization

This thesis points to a possible weakness of the empirical literature on corruption and government spending. That corruption affects the composition of government spending, and in particular that it affects education and health spending adversely, seems to be empirically well established. However, there exist additional literature closely related to corruption and government spending, treating (i) a relationship between corruption and decentralization, and (ii) a relationship between decentralization and government spending. These relationships are not accounted for in the literature on corruption and government spending. If corruption and decentralization are correlated, and in addition decentralization affects government spending, then omitting decentralization might cause biased results. In order to test for possible omitted variable bias, a simple cross-country analysis is performed. Three versions of the classical linear regression model are specified, and estimated in Stata/MP 10 using ordinary least squares estimation. In the first version, the relationship between corruption and government spending, excluding decentralization, is investigated. In the next version, the model is extended to include decentralization as explanatory variable, while in the third version, an interaction term between corruption and decentralization is included as well. These models are then estimated using different measures of government spending on education and health as dependent variables. The results support the findings that corruption adversely affects government spending on health and education. These findings seem to be very robust, also when decentralization is included as an explanatory variable. Nevertheless, there are indications that there might be an interaction effect between corruption and decentralization. Specifically, government spending on health seems to be affected more negatively by corruption in decentralized countries. There is a need to investigate this further. In addition, an explanation of this interaction effect remains to establish. Meanwhile, an intuitive guess is that corruption can take different forms at different government levels, and that these different forms of corruption may have different effects on health spending.
Effect of Altering Dietary n-6:n-3 Polyunsaturated Fatty Acid Ratio with Plant and Marine-Based Supplement on Biomarkers of Bone Turnover in Healthy Adults

Although there is accumulating evidence for a protective role of n-3 polyunsaturated fatty acids (n-3 PUFAs) on bone health, there are limited studies that examine the effect of altering dietary n-6:n-3 PUFA ratio with plant and marine sources of n-3 PUFA on bone health. Healthy adults (n = 24) were randomized into an eight-week crossover study with a four-week washout between treatments, with each subject consuming three of four diets. The four diets differed in the dietary n-6:n-3 PUFA ratios and either had an algal oil supplement added or not: (Control diet (10:1); α-linolenic acid (ALA) diet (2:1); Eicosapentaenoic acid/Docosahexaenoic acid (EPA/DHA) diet (10:1 plus supplement (S) containing EPA/DHA; Combination diet (2:1 + S)). The supplement was microalgae oil that provided 1 g EPA + DHA/day. Flaxseed oil and walnuts provided 8.6 g of ALA/day in the 2:1 diets. Serum levels of c-telopeptide (CTX), procollagen Type I N-terminal peptide, and osteocalcin showed significant correlation with age but none of the bone markers or peroxisomal proliferator-activated receptor-γ mRNA expression was significantly different between the diets. Serum CTX was negatively associated with red blood cell membrane linoleic acid and ALA and positively associated with membrane DHA. Neither altering dietary n-6:n-3 PUFA ratio from a 10:1 to a 2:1 ratio nor adding EPA/DHA supplement significantly changed bone turnover in the short term in healthy adults.
RECREATION AND RISK AROUND LOS ALAMOS: ARE HISPANICS MORE AT RISK?

The Department of Energy (DOE) and other federal facilities are involved in massive remediation and restoration efforts on lands that may eventually be turned over for recreation or other uses by the public. In addition, other sites are expected to continue their ongoing missions, but recreation may be sanctioned, or not discouraged, on their remediated lands. Understanding the amount and types of recreation of regional residents who might use such lands, as well as their willingness to use these lands, is critical to determining both cleanup and restoration standards, and potential future risk. In this article the recreational rates, current recreational use, and willingness to recreate on Los Alamos National Laboratory in New Mexico are examined for 356 people interviewed at a well-attended gun show in Albuquerque, NM. There were few significant ethnic differences in recreational rates, although Hispanics had higher fishing rates and lower birdwatching rates than whites. Women hunted less, and photographed more, than men. Younger people fished and hunted more, and bird-watched less, than older people. There were no differences in recreational rates as a function of income or education. These data can be used for understanding potential exposure of people in the vicinity of Los Alamos.
Targeted Spinal Cord Therapeutics Delivery: Stabilized Platform and Microelectrode Recording Guidance Validation

Background/Aims: No validated delivery technique exists for accurate, reproducible delivery of biological therapies to discrete spinal cord targets. To address this unmet need, we have constructed a stabilized platform capable of supporting physiologic mapping, through microelectrode recording, and cellular or viral payload delivery to the ventral horn. Methods: A porcine animal model (n = 7) has been chosen based upon the inherent morphologic similarities between the human and porcine spine. Animals underwent physiologic mapping and subsequent microinjection of a green-fluorescent-protein-labeled cell suspension. Sacrifice (t = 3 h) was performed immediately following behavioral assessment. Results: Histologic analysis has supported our ability to achieve localization to the ipsilateral ventral horn in the spinal cord. Complications included death due to malignant hyperthermia (n = 1), hindlimb dysfunction attributable to epidural hematoma (n = 1), and hindlimb dysfunction attributable to cord penetration (n = 2). Conclusions: These results indicate an ability to achieve accurate targeting, but the elevated incidence of neurologic morbidity will require further studies with longer follow-ups that incorporate procedural and equipment modifications that will allow for a reduced number of cord penetrations and will account for observed cardiorespiratory-associated cord movement. These initial results reinforce the challenges of translating biological restorative therapies from small to large animal models and ultimately to humans.
Institutions, Contracts and Organizations

This outstanding book presents new original contributions from some of the world’s leading economists including Ronald Coase, Douglass C. North, Masahiko Aoki, Oliver E. Williamson and Harold Demsetz. It demonstrates the extent and depth of the New Institutional Economics research programme which is having a worldwide impact on the economics profession.
Myofibroblasts revert to an inactive phenotype during regression of liver fibrosis

Myofibroblasts produce the fibrous scar in hepatic fibrosis. In the carbon tetrachloride (CCl4) model of liver fibrosis, quiescent hepatic stellate cells (HSC) are activated to become myofibroblasts. When the underlying etiological agent is removed, clinical and experimental fibrosis undergoes a remarkable regression with complete disappearance of these myofibroblasts. Although some myofibroblasts apoptose, it is unknown whether other myofibroblasts may revert to an inactive phenotype during regression of fibrosis. We elucidated the fate of HSCs/myofibroblasts during recovery from CCl4- and alcohol-induced liver fibrosis using Cre-LoxP–based genetic labeling of myofibroblasts. Here we demonstrate that half of the myofibroblasts escape apoptosis during regression of liver fibrosis, down-regulate fibrogenic genes, and acquire a phenotype similar to, but distinct from, quiescent HSCs in their ability to more rapidly reactivate into myofibroblasts in response to fibrogenic stimuli and strongly contribute to liver fibrosis. Inactivation of HSCs was associated with up-regulation of the anti-apoptotic genes Hspa1a/b, which participate in the survival of HSCs in culture and in vivo.