Miracle-dz/UniFAR-FTUs huggingface dataset:
Reduction of microtubule catastrophe events by LIS1, platelet‐activating factor acetylhydrolase subunit

Forming the structure of the human brain involves extensive neuronal migration, a process dependent on cytoskeletal rearrangement. Neuronal migration is believed to be disrupted in patients exhibiting the developmental brain malformation lissencephaly. Previous studies have shown that LIS1, the defective gene found in patients with lissencephaly, is a subunit of the platelet‐activating factor acetylhydrolase. Our results indicated that LIS1 has an additional function. By interacting with tubulin it suppresses microtubule dynamics. We detected LIS1 interaction with microtubules by immunostaining and co‐assembly. LIS1–tubulin interactions were assayed by co‐immunoprecipitation and by surface plasmon resonance changes. Microtubule dynamic measurements in vitro indicated that physiological concentrations of LIS1 indeed reduced microtubule catastrophe events, thereby resulting in a net increase in the maximum length of the microtubules. Furthermore, the LIS1 protein concentration in the brain, measured by quantitative Western blots, is high and is approximately one‐fifth of the concentration of brain tubulin. Our new findings show that LIS1 is a protein exhibiting several cellular interactions, and the interaction with the cytoskeleton may prove to be the mode of transducing a signal generated by platelet‐activating factor. We postulate that the LIS1–cytoskeletal interaction is important for neuronal migration, a process that is defective in lissencephaly patients.
Queueing analysis for OFDM subcarrier allocation in broadband wireless multiservice networks

In this paper, we perform a tele-traffic queueing analysis for OFDM subcarrier allocation in wireless multiservice networks. For this purpose two call admission control algorithms, the batch blocking scheme and the partial blocking scheme, are proposed. Call connection requests are classified into two different types, narrow-band and wide-band. For either class of calls, the traffic process is characterized as batch arrival, as each call may request multiple subcarriers to satisfy its quality-of-service (QoS) requirements. The batch size is a random variable which obeys a probability mass function (pmf) with a realistic maximum value. In addition, the service times for various call classes are different. Consequently, an OFDM-based broadband wireless multiservice network can be formulated as a multiclass multiserver batch arrival queueing system. The methodology and results are further generalized into a multiple-class scenario with service priority provision. Formulae are developed for evaluation of the following performance metrics: the probability that a call will be blocked, the average number of subcarriers used, and bandwidth utilization. Numerical results are presented to demonstrate the interactions between key parameters and performance metrics. The analytical model was validated by the simulation results, showing the fact that it can be used as an efficient tool for design of future-generation broadband wireless access networks.
A 135‐kd membrane protein of intercellular adherens junctions.

We report here on a new 135‐kd membrane protein which is specifically associated with intercellular adherens‐type junctions. This surface component was identified by a monoclonal antibody, ID‐7.2. 3, raised against detergent‐extracted components of membranes of chicken cardiac muscle rich in intercalated discs. The antibodies stain extensively adherens junctions in intact cardiac muscle and in lens, as well as in cultured cells derived from these tissues. In living cultured cells only very little immunolabelling was obtained with ID‐7.2. 3 antibodies, probably due to the limited accessibility of the antibodies to the intercellular gap. However, upon the removal of extracellular Ca2+ ions a dissociation of the junction occurred, leading to the rapid exposure of the 135‐kd protein. Immunoelectron microscopic labelling of EGTA‐treated, or detergent‐permeabilized cells indicated that the antigen is found along the plasma membrane and highly enriched in contact areas. Double immunolabelling for both the 135‐kd protein and vinculin pointed to the close association of the two in intercellular junctions and to the apparent absence of the former protein from the vinculin‐rich focal contacts of cultured cells and from dense plaque of smooth muscle. Immunoblotting indicated that the 135‐kd protein is present in many tissues but is particularly enriched in heart, lens and brain.
Mahtab at SemEval-2017 Task 2: Combination of Corpus-based and Knowledge-based Methods to Measure Semantic Word Similarity

In this paper, we describe our proposed method for measuring semantic similarity for a given pair of words at SemEval-2017 monolingual semantic word similarity task. We use a combination of knowledge-based and corpus-based techniques. We use FarsNet, the Persian Word Net, besides deep learning techniques to extract the similarity of words. We evaluated our proposed approach on Persian (Farsi) test data at SemEval-2017. It outperformed the other participants and ranked the first in the challenge.
Physiologic Responses of Firefighter Recruits During a Supervised Live-Fire Work Performance Test

Del Sal, M, Barbieri, E, Garbati, P, Sisti, D, Rocchi, MBL, and Stocchi, V. Physiologic responses of firefighter recruits during a supervised live-fire work performance test. J Strength Cond Res 23(8): 2396-2404, 2009-The aim of this research was to determine physiologic responses to typical activities of military Italian firefighters. Heart rate (HR), metabolic equivalent units (MET), skin temperature ( T.Sk.), and galvanic skin response (GSR) were measured in 13 firefighters (age = 36.3 ± 6.9 yr; period of military fire service = 16.8 ± 7 yr) during the acclimation phase (5 min standing, still dressed in their protective clothing), the work phase (simulated firefighting situations), and the following 24 hours. Multivariate linear step-wise regression showed that body mass index was highly correlated with mean and minimal HR values during the acclimation phase (beta 0.59, p < 0.001; beta 1.90, p = 0.003) and with mean and maximum HR values during the work phase (beta 1.08, p = 0.05; beta 1.17, p = 0.04), increasing cardiovascular stress. Firefighting tasks were associated with high energy expenditure. The minimum and mean MET values, during the acclimation phase, were significantly correlated with age (beta 0.49, p = 0.008 and beta 0.46, p < 0.01). During the work phase, maximal MET values were also strongly correlated with weight (beta 0.51, p = 0.03). No correlations between predictor and dependent variables were found for GSR; however, the high GSR levels recorded during the work phase returned to normal values only 12 hours after completion of the work phase, indicating that high levels of psychological stress remain, even after physical recovery. The data obtained in this study demonstrate that the physical fitness and anthropometric characteristics of firefighters influence the performance of firefighting tasks. These results may be useful to set up specific training that meets the real needs of firefighters in terms of physical fitness.
A novel quasi-SEPIC high-voltage boost DC-DC converter

This paper proposes a modified coupled-inductor SEPIC dc-dc converter for low power and high voltage gain applications such as for piezoelectric drive systems. The converter uses the same components as of SEPIC converter with an additional diode. Compared to conventional topologies with similar voltage gain expression, the proposed topology uses less components to achieve same or even higher voltage gain. This helps to design a very compact and light weight converter with higher power density at lower cost. Due to brevity, the principle of operation, theoretical analysis and comparison supported by some key experimental results of 5 W prototype are presented in brief.
An inducible lambdoid prophage encoding cytolethal distending toxin (Cdt-I) and a type III effector protein in enteropathogenic Escherichia coli

Cytolethal distending toxins (CDTs) are inhibitory cyclomodulins, which block eukaryotic cell proliferation and are produced by a diverse group of Gram-negative bacteria, including Escherichia coli strains associated with intestinal and extraintestinal infections. However, the mode of transmission of the toxin gene clusters among diverse bacterial pathogens is unclear. We found that Cdt-I produced by enteropathogenic E. coli strains associated with diarrhea is encoded by a lambdoid prophage, which is inducible and infectious. The genome of Cdt-I converting phage (CDT-1Φ) comprises 47,021 nucleotides with 60 predicted ORFs organized into six genomic regions encoding the head and tail, virulence, integrase, unknown functions, regulation, and lysis. The genomic organization of CDT-1Φ is similar to those of SfV, a serotype-converting phage of Shigella flexneri, and UTI89, a prophage identified in uropathogenic E. coli. Besides the cdtI gene cluster, the virulence region of CDT-1Φ genome contains sequences homologous to a truncated cycle inhibiting factor and a type 3 effector protein. Mutation analysis of susceptible E. coli strain C600 suggested that the outer membrane protein OmpC is a putative receptor for CDT-1Φ. CDT-1Φ genome was also found to integrate into the host bacterial chromosome forming lysogens, which produced biologically active Cdt-I. Furthermore, phage induction appeared to cause enhanced toxigenicity of the E. coli strains carrying lysogenic CDT-1Φ. Our results suggest that CDT-1Φ is the latest member of a growing family of lambdoid phages encoding bacterial cyclomodulins and that the phage may have a role in horizontal transfer of these virulence genes.
Suppression of VEGF-induced angiogenesis and tumor growth by Eugenia jambolana, Musa paradisiaca, and Coccinia indica extracts

Abstract Context: Abnormal angiogenesis and evasion of apoptosis are hallmarks of cancer. Accordingly, anti-angiogenic and pro-apoptotic therapies are effective strategies for cancer treatment. Medicinal plants, namely, Eugenia jambolana Lam. (Myrtaceae), Musa paradisiaca L. (Musaceae), and Coccinia indica Wight & Arn. (Cucurbitaceae), have not been greatly investigated for their anticancer potential. Objective: We investigated the anti-angiogenic and pro-apoptotic efficacy of ethyl acetate (EA) and n-butanol (NB) extracts of E. jambolana (seeds), EA extracts of M. paradisiaca (roots) and C. indica (leaves) with respect to mammary neoplasia. Materials and methods: Effect of extracts (2–200 μg/mL) on cytotoxicity and MCF-7, MDA-MB-231 and endothelial cell (EC) proliferation and in vitro angiogenesis were evaluated by MTT, 3[H]thymidine uptake and EC tube formation assays, respectively. In vivo tumour proliferation, VEGF secretion and angiogenesis were assessed using the Ehrlich ascites tumour (EAT) model followed by rat corneal micro-pocket and chicken chorioallantoic membrane (CAM) assays. Apoptosis induction was assessed by morphological and cell cycle analysis. Results: EA extracts of E. jambolana and M. paradisiaca exhibited the highest cytotoxicity (IC50 25 and 60 μg/mL), inhibited cell proliferation (up to 81%), and tube formation (83% and 76%). In vivo treatment reduced body weight (50%); cell number (16.5- and 14.7-fold), secreted VEGF (∼90%), neoangiogenesis in rat cornea (2.5- and 1.5-fold) and CAM (3- and 1.6-fold) besides EAT cells accumulation in sub-G1 phase (20% and 18.38%), respectively. Discussion and conclusion: Considering the potent anti-angiogenic and pro-apoptotic properties, lead molecules from EA extracts of E. jambolana and M. paradisiaca can be developed into anticancer drugs.
Assessing Depression in the Cardiac Patient

Depression is a well-established risk factor for cardiovascular disease-related morbidity and mortality. It is common to screen for depression in patients undergoing coronary revascularization prior to revascularization; however, the validity of this assessment is unclear as some patients may experience transient, reactive depression rather than persistent depression. The authors evaluated whether an initial or 1-month postprocedure screen was optimal for identifying consistently depressed patients. Depression at 1-month postprocedurewas a stronger predictor of depression at months 2 to 6 than baseline depression. After adjusting potential confounding variables, there was a much stronger relationship between 1-month and 6-month depression status (OR = 28.7 if depressed at 1 month, p < .001) than between baseline and 6-month depression status (OR = 6.5 if depressed at baseline, p < .001). Screening for depression at the time of revascularization is not as predictive of depression at 6 months as it is 1 month postprocedure.
Coordination of Generation Scheduling with PEVs Charging in Industrial Microgrids

Conventional industrial microgrids (IMGs) consist of factories with distributed energy resources (DERs) and electric loads that rely on combined heat and power (CHP) systems while the developing IMGs are expected to also include renewable DERs and plug-in electric vehicles (PEVs) with different vehicle ratings and charging characteristics. This paper presents an electricity and heat generation scheduling method coordinated with PEV charging in an IMG considering photovoltaic (PV) generation systems coupled with PV storages. The proposed method is based on dynamic optimal power flow (DOPF) over a 24-hour period and includes security-constrained optimal power flow (SCOPF), IMG's factories constraints, PV storage constraints and PEVs dynamic charging constraints. It will utilize the generators waste heat to fulfill thermal requirements while considering the status of renewable DERs to decrease the overall cost of IMGs. To demonstrate the effectiveness of the proposed method, detailed simulation results are presented and analyzed for an 18-bus IMG consisting of 12 factories and 6 types of PEVs without/with PV generation systems operating in grid-connected and stand-alone modes. The main contribution is including PEVs with dynamic constraints that have changed the nature of scheduling formulation from a simple hourly OPF to a dynamic OPF.
Ethical issues experienced by healthcare workers in nursing homes

Background: Ethical issues are increasingly being reported by care-providers; however, little is known about the nature of these issues within the nursing home. Ethical issues are unavoidable in healthcare and can result in opportunities for improving work and care conditions; however, they are also associated with detrimental outcomes including staff burnout and moral distress. Objectives: The purpose of this review was to identify prior research which focuses on ethical issues in the nursing home and to explore staffs’ experiences of ethical issues. Methods: Using a systematic approach based on Aveyard (2014), a literature review was conducted which focused on ethical and moral issues, nurses and nursing assistants, and the nursing home. Findings: The most salient themes identified in the review included clashing ethical principles, issues related to communication, lack of resources and quality of care provision. The review also identified solutions for overcoming the ethical issues that were identified and revealed the definitional challenges that permeate this area of work. Conclusions: The review highlighted a need for improved ethics education for care-providers.
Form frame line detection with directional single-connected chain

In this paper, a novel form frame line detection algorithm is proposed based on the directional single-connected chain (DSCC). Defined as an array of black pixel run-lengths, DSCC works very well as an image structure element or vector in our vectorization algorithm. By merging multiple DSCCs under some constraints, we are able to extract the form frame lines automatically yet fast. The speed of our algorithm is comparable with some well-known projection methods. Experiments show that our algorithm is fast, resistant to moderate serious line breaks and can detect diagonal lines with any angle.
Mangiferin modulation of metabolism and metabolic syndrome

The recent emergence of a worldwide epidemic of metabolic disorders, such as obesity and diabetes, demands effective strategy to develop nutraceuticals or pharmaceuticals to halt this trend. Natural products have long been and continue to be an attractive source of nutritional and pharmacological therapeutics. One such natural product is mangiferin (MGF), the predominant constituent of extracts of the mango plant Mangifera indica L. Reports on biological and pharmacological effects of MGF increased exponentially in recent years. MGF has documented antioxidant and anti‐inflammatory effects. Recent studies indicate that it modulates multiple biological processes involved in metabolism of carbohydrates and lipids. MGF has been shown to improve metabolic abnormalities and disorders in animal models and humans. This review focuses on the recently reported biological and pharmacological effects of MGF on metabolism and metabolic disorders. © 2016 BioFactors, 42(5):492–503, 2016
Frequency-fluctuation model for line-shape calculations in plasma spectroscopy.

A model is developed that permits the calculation of the radiation emitted by complex or highly charged ions in a plasma. The model is based on the usual separation of the plasma-emitter interaction into the homogeneous broadening effects of the fast electrons and the inhomogeneous broadening arising from slow ions. For plasma conditions where the ion motion can be neglected, the spectrum is the usual static line shape. To account for ion dynamics, the frequency-fluctuation model is introduced by decomposing the line shape of each radiative transition into a sum of radiative channels that are associated with the smallest observable inhomogeneities that form the static profile. The fluctuations of the ion microfield, the ion dynamics effect, is modeled by an exchange process between the static radiative channels. This results in both a smoothing and an overall coalescence of the radiative channels and depends strongly on an averaged characteristic fluctuation rate associated with the dynamics of the interaction of the local plasma microfield with the ion. This rate is formally related to the double-time field-field correlation function behavior. This stochastic model of the observed frequency fluctuations permits fast and accurate calculations of the emitted spectral profiles, including ion dynamics emitted by complex ions in a wide range of plasma conditions.
A scalable flow rule translation implementation for software defined security

Software defined networking brings many possibilities to network security, one of the most important security challenge it can help with is the possibility to make network traffic pass through specific security devices, in other words, determine where to deploy these devices logically. However, most researches focus on high level policy and interaction framework but ignored how to translate them to low-level OpenFlow rules with scalability. We analyze different actions used in common security scenarios and resource constraints of physical switch. Based on them, we propose a rule translation implementation which can optimize the resource consumption according to different actions by selecting forward path dynamically.
A Compact, Highly Efficient and Flexible Polymer Ultra-Wideband Antenna

A compact, highly efficient and flexible ultra-wideband antenna operating from 3 to 20 GHz is proposed in this letter. The antenna is completely made from polymer comprising a patterned conductive polymer (PEDOT) thin film attached to a transparent sticky tape substrate. The overall dimension is less than a quarter-wavelength at the lowest frequency of operation and the device reaches a radiation efficiency of over 85% averaged throughout the frequency band. The antenna performs well under various bending conditions as demonstrated by measurements. The realized antenna offers great mechanical flexibility and robustness which indicates its promising potential for possible seamless integration in flexible electronics.
Nanotechnology-Based Terahertz Biological Sensing: A review of its current state and things to come

In recent decades, terahertz (THz) technology has seen rapid development and significant application in the biological sensing field. In this process, nanotechnology has been constantly creating possibilities for THz technology advancement. Here, we discuss common THz biosensing techniques and limitations and focus on how state-of-the-art nanotechnology helps to improve THz biological imaging capability in terms of providing advanced THz systems and superior imaging quality. Cutting-edge research progress made in nanotechnology and THz technology cross-point have greatly reshaped the THz biosensing landscape.
Residues on the Dimer Interface of SARS Coronavirus 3C-like Protease: Dimer Stability Characterization and Enzyme Catalytic Activity Analysis

Abstract 3C-like protease (3CLpro) plays pivotal roles in the life cycle of severe acute respiratory syndrome coronavirus (SARS-CoV) and only the dimeric protease is proposed as the functional form. Guided by the crystal structure and molecular dynamics simulations, we performed systematic mutation analyses to identify residues critical for 3CLpro dimerization and activity in this study. Seven residues on the dimer interface were selected for evaluating their contributions to dimer stability and catalytic activity by biophysical and biochemical methods. These residues are involved in dimerization through hydrogen bonding and broadly located in the N-terminal finger, the α-helix A′ of domain I, and the oxyanion loop near the S1 substrate-binding subsite in domain II. We revealed that all seven single mutated proteases still have the dimeric species but the monomer–dimer equilibria of these mutants vary from each other, implying that these residues might contribute differently to the dimer stability. Such a conclusion could be further verified by the results that the proteolytic activities of these mutants also decrease to varying degrees. The present study would help us better understand the dimerization-activity relationship of SARS-CoV 3CLpro and afford potential information for designing anti-viral compounds targeting the dimer interface of the protease.
The TDT and other family-based tests for linkage disequilibrium and association.

The transmission/disequilibrium test (TDT) was introduced several years ago by Spielman et al. as a test for linkage between a complex disease and a genetic marker. The original intended use of the TDT was to test for linkage with a marker located near a candidate gene, in cases where disease association already had been found. However, even if prior evidence for association is absent, the TDT is valid and can be used to test any marker (or a set of markers) for which data are available from parents and one or more affected offspring. Other tests that focus on association itself - but that, like the TDT, are applied to data from nuclear families - also have been proposed. In addition, several papers have appeared recently that discuss extensions and properties of these tests and of the TDT. Since the aim of both the TDT and the association tests is to locate genes that contribute to disease susceptibility, a number of questions about their differences have arisen. In this review, we compare the properties of the TDT with those of family-based tests of association, and we comment on issues regarding the use of these tests. 22 refs., 2 tabs.