Miracle-dz/UniFAR-FTUs huggingface dataset:
Role of interleukin 16 in multiple myeloma.

BACKGROUND Multiple myeloma is a malignancy characterized by the expansion of a plasma cell clone that localizes to the human bone marrow. Myeloma cells and bone marrow stromal cells produce soluble factors that promote the survival and progression of multiple myeloma. Interleukin 16 (IL-16) is involved in regulating the migration and proliferation of normal leukocytes. However, the role of IL-16 in human cancers, including multiple myeloma, is unclear. METHODS We investigated IL-16 expression in cell lines (n = 10) and in the bone marrow of myeloma patients (n = 62) and healthy bone marrow donors (n = 12) by quantitative reverse transcription-polymerase chain reaction, immunoblot analysis, enzyme-linked immunosorbent assay, flow cytometry, and immunohistochemistry. Transfection of two human multiple myeloma cell lines with small interfering RNAs was used to examine the effect of IL-16 gene silencing on apoptosis by flow cytometry, on proliferation by bromodeoxyuridine incorporation, and on colony formation. Protein neutralization assays were performed by treating multiple myeloma cells with a monoclonal antibody against the carboxyl-terminal fragment of IL-16. All statistical tests were two-sided. RESULTS IL-16 was strongly overexpressed in the bone marrow of myeloma patients compared with healthy donors. Myeloma cell lines as well as primary tumor cells from myeloma patients constitutively expressed IL-16 and its receptors CD4 and/or CD9 and spontaneously secreted soluble IL-16. Silencing of IL-16 reduced the proliferative activity of myeloma cells by approximately 80% compared with untreated cells (mean relative proliferative activity IL-16 siRNA vs untransfected cells, EJM cells: 20.1%, 95% confidence interval [CI] = 14.3% to 26.0%, P = .03; KMS-12-BM cells: 22.8%, 95% CI = 5.5% to 40.0%, P = .04), and addition of a recombinant carboxyl-terminal IL-16 peptide reversed that effect. A monoclonal antibody directed against IL-16 or its receptors had a comparably strong growth-inhibiting effect on the tumor cells. CONCLUSIONS IL-16 is an important growth-promoting factor in multiple myeloma and a candidate for novel diagnostic, prognostic, and therapeutic applications for this incurable human malignancy.
Measuring Product Material Footprint as New Life Cycle Impact Assessment Method: Indicators and Abiotic Characterization Factors

The global economy is using growing amounts of natural resources such as raw materials, water, and land by making and using goods, services, and infrastructure. Aspirations on international, regional, and national levels e.g., the Sustainable Development Goals, the EU flagship initiative Roadmap to a Resource Efficient Europe or the German Program for Resource Efficiency are showing an urgent need to bring the global raw material use down to sustainable levels. An essential prerequisite to identify resource efficient options and to implement resource efficiency measures and solutions is the ability to compare different products or services regarding their raw material use. Until today, there is no internationally standardized approach defined and no software supported calculation method including the necessary data basis available to measure the raw material intensity of products. A new life cycle impact assessment (LCIA) method Product Material Footprint PMF is described. Two indicators are used to quantify the PMF: the Raw Material Input RMI and the Total Material Requirement TMR. The calculation of global median values for the characterization factors CFRMI and CFTMR of abiotic materials was done based on different databases. This article presents the methodological approach of the PMF, the calculation results for CFRMI of 42 abiotic materials and CFTMR of 36 abiotic materials, and the implementation of the LCIA method into the software openLCA for use with the ecoinvent database.
Segregation and linkage analysis in five manic depression pedigrees excludes the 5HT1a receptor gene (HTR1A)

Five kindreds selected through probands attending an Icelandic hospital were recruited for linkage studies of manic depression. The rates of affection were equal for males and females and the age of onset appeared to be predominantly in early adult life, since prevalence did not rise appreciably with age. A complex segregation analysis was performed using the computer program pointer to obtain maximum likelihood estimates of the contributions to liability from multifactorial transmission and a single major locus. Likelihood ratios between models supported a role for a single major locus which was dominant and had moderately high penetrance with, in the case of unipolar illness, additional multifactorial transmission. The bestfitting parameters were used to devise a transmission model for linkage analysis. Three markers on chromosome 5 were studied, at D5S76, D5S6 and D5S39. Strongly negative lod scores were obtained which were less than ‐2 over a distance of 40 cM, which included the region to which the gene for the 5HT1a receptor has been mapped.
Characteristics and Outcome of Patients With AIDS in Dutch ICUs Between 1997 and 2014*

Objective:Knowledge on characteristics and outcome of ICU patients with AIDS is highly limited. We aimed to determine the main reasons for admission and outcome in ICU patients with AIDS and trends over time therein. Design:A retrospective study within the Dutch National Intensive Care Evaluation registry. Setting:Dutch ICUs. Patients:We used data collected between 1997 and 2014. Characteristics of patients with AIDS were compared with ICU patients without AIDS, matched for age, sex, admission type, and admission year. Joinpoint regression analysis was applied to study trends over time. Interventions:None. Measurements and Main Results:We included 1,127 patients with AIDS and 4,479 matched controls. The main admission diagnoses of patients with AIDS were respiratory infection (28.6%) and sepsis (16.9%), which were less common in controls (7.7% and 7.5%, respectively; both p < 0.0001). Patients with AIDS had increased severity of illness and in-hospital mortality (28.2% vs 17.8%; p < 0.0001) compared with controls, which was associated with a higher rate of infections at admission in patients with AIDS (58.4% vs 25.5%). Over time, the proportion of patients with AIDS admitted with an infection decreased (75% in 1999 to 56% in 2013). Mortality declined in patients with AIDS (39% in 1999 to 16% in 2013), both in patients with or without an infection. Mortality also declined in matched controls without AIDS, but to a lesser extent. Conclusion:Infections are still the main reason for ICU admission in patients with AIDS, but their prevalence is declining. Outcome of patients with AIDS continued to improve during a time of widespread availability of combination antiretroviral therapy, and mortality is reaching levels similar to ICU patients without AIDS.
Semantic Human Action Classification Based on Energy-Action Model

Human action classification for interpreting at a semantic level has been and still a highly interesting and important research topic. The research results are capable of analyzing human action that we visually perceive in many aspects. Therefore the research requires an effective and competent approach to accurately interpret human action. In this paper, we present a novel model called the energy-action (EA) model for producing more semantic classification. The EA model is based on the fundamental concepts of biomechanics that human movement in different classes is likely to spend different amounts of energy. The EA model is classified by using feedforward backpropagation neural network (FBNN) to obtain from the 5-fold cross validation. Experimental results show that the proposed provides much more authentic meaning of human actions
Effect of Sodium on Vasoconstriction and Angiotensin II Type 1 Receptor mRNA Expression in Cold‐induced Hypertensive Rats

Angiotensin II and sodium play an important pathogenetic role in several models of hypertension. Now, we investigated the effects of sodium on vasoconstriction and angiotensin II type 1 (AT1) and type 2 (AT2) receptor mRNA expression in aortic vessels from cold‐induced hypertensive rats. Wistar rats on low sodium and high sodium diet were exposed to cold‐stress for 8 weeks. The effects of angiotensin II infusion on mean arterial blood pressure were investigated in these rats. In addition, angiotensin II induced contraction was measured using aortic rings. Expression of AT1 receptor mRNA and AT2 receptor mRNA was assessed in aortic vessels by reverse transcription polymerase chain reaction. After infusion of angiotensin II mean arterial blood pressure in cold‐induced hypertensive rats on high sodium diet was significantly higher compared to cold‐induced hypertensive rats on low sodium diet (p < 0.05). Angiotensin II‐induced contraction of aortic rings was significantly higher in cold‐induced hypertensive rats on high sodium diet compared to cold‐induced hypertensive rats on low sodium diet (2.39 ± 0.03 g vs. 2.21 ± 0.04 g, n = 12, p < 0.01). Angiotensin AT1 receptor mRNA was significantly higher in cold‐induced hypertensive rats on high sodium diet compared to cold‐induced hypertensive rats on low sodium diet (p < 0.05). It is concluded that in this nongenetic, nonsurgical animal model of cold‐induced hypertension increased vasoconstriction and increased AT1 receptor mRNA expression in aortic vessels are dependent on sodium intake.
Mesenchymal Stromal/Stem Cells: A New Era in the Cell-Based Targeted Gene Therapy of Cancer

In recent years, in light of the promising potentials of mesenchymal stromal/stem cells (MSCs) for carrying therapeutic anticancer genes, a complete revisitation on old chemotherapy-based paradigms has been established. This review attempted to bring forward and introduce the novel therapeutic opportunities of using genetically engineered MSCs. The simplicities and advantages of MSCs for medical applications make them a unique and promising option in the case of cancer therapy. Some of the superiorities of using MSCs as therapeutic gene micro-carriers are the easy cell-extraction procedures and their abundant proliferation capacity in vitro without losing their main biological properties. Targeted therapy by using MSCs as the delivery vehicles of therapeutic genes is a new approach in the treatment of various types of cancers. Some of the distinct properties of MSCs, such as tumor-tropism, non-immunogenicity, stimulatory effect on the anti-inflammatory molecules, inhibitory effect on inflammatory responses, non-toxicity against the normal tissues, and easy processes for the clinical use, have formed the basis of attention to MSCs. They can be easily used for the treatment of damaged or injured tissues, regenerative medicine, and immune disorders. This review focused on the drugability of MSCs and their potential for the delivery of candidate anticancer genes. It also briefly reviewed the vectors and methods used for MSC-mediated gene therapy of malignancies. Also, the challenges, limitations, and considerations in using MSCs for gene therapy of cancer and the new methods developed for resolution of these problems are reviewed.
Engaging families through observation and reflection: a collaborative approach to making learning visible

ABSTRACT Early childhood education is based on the assertion that young children are better prepared for school and life when educators and parents work together on their behalf. Family engagement is a central force in the education of young children, yet research suggests that parents and teachers often do not collaborate, and this can have an adverse impact on young children’s learning outcomes. This brief explores a new observation approach, referred to as Journey of Discoveries, to examine its influence on family engagement and its potential use as a collaborative tool for educators and parents to gauge children’s learning and development. Journey of Discoveries was tested with volunteer families and teachers in three select Early Head Start programs in California in 2014–2015. Responses from participants illustrated seven themes or potential pathways emerging from the approach that support family engagement, including: (a) teachers’ and parents’ celebration of children’s learning, (b) emotional responses to children’s learning, (c) importance of sharing learning as stories, (d) process of reflection, (e) holistic viewing of children’s learning, (f) the honoring of diverse voices, and (g) parents’ roles as leaders. The practical and policy implications of the Journey of Discoveries approach are also discussed.
HuD Promotes Progression of Oral Squamous Cell Carcinoma

Head and neck cancer, including oral squamous cell carcinoma (OSCC), ranks as the sixth most common malignancy worldwide. Overall 5-year survival rates of OSCC have not significantly improved during the past 3 decades and the 5-year survival rate is less than 50%. Several invasion grading systems have been employed in OSCC, however, their utility is still controversial. HuD belongs to the Hu protein family and acts as an RNA-binding protein involved in mRNA stability and translational regulation. Although HuD has a pivotal role for neuronal differentiation, the functional role of HuD in OSCCs is still unclear. In this study, we examined HuD expression in 82 OSCC cases. Expression of HuD was observed in 36.6% of OSCCs and significantly associated with histological differentiation, nodal metastasis and mode of invasion. HuD expression in high-metastatic HSC3 cells was higher than in low-metastatic HSC4 cells, and inhibition of invasion ability and activation of caspase-3 were shown by HuD siRNA-treated HSC3 cells. Furthermore, we clarified that HuD regulates expression of vascular endothelial growth factor (VEGF)-A, VEGF-D, matrix metallopeptidase (MMP)-2 and MMP-9. These results suggest that HuD is a useful diagnostic and therapeutic target in OSCCs.
Epidural analgesia in labor: an evaluation of risks and benefits.

BACKGROUND Intrapartum epidural analgesia has become increasingly popular because it is the most effective method of providing pain relief during labor. Much attention is given to its safety and efficacy, and many health care providers and consumers are unaware of its potential drawbacks. This article reviews the literature about the effects of epidural analgesia on the mother and infant. METHODS We performed a computer-assisted MEDLINE search for articles and a review of bibliographies from articles on epidural analgesia. When reported data were incomplete, authors were contacted for more detailed information. RESULTS The most common procedure-related complications, hypotension, inadvertent dural puncture, and headache, are easily treated and usually self-limited. Permanent morbidity and mortality are rare. Retrospective studies and randomized controlled trials both demonstrate that epidural analgesia is associated with increases in duration of labor, instrument vaginal delivery, and cesarean birth. To date only three trials randomized patients to narcotic versus epidural groups, and all showed a twofold to threefold increase in cesarean section for dystocia. Limiting epidural use in nulliparous labor and delaying its placement until after 5 cm of cervical dilation may reduce the risk of operative intervention for dystocia. Epidural analgesia may also increase intervention for fetal distress. Several studies show its association with maternal fever in labor. Its association with chronic back pain, neonatal behavioral changes, and maternal-infant bonding are more tenuous and require further study. CONCLUSIONS Epidural analgesia is a safe and effective method of relieving pain in labor, but is associated with longer labor, more operative intervention, and increases in cost. It must remain an option; however, caregivers and consumers should be aware of associated risks. Women should be counseled about these risks and other pain-relieving options before the duress of labor.
Loss of Type I Collagen Telopeptide Lysyl Hydroxylation Causes Musculoskeletal Abnormalities in a Zebrafish Model of Bruck Syndrome

Bruck syndrome (BS) is a disorder characterized by joint flexion contractures and skeletal dysplasia that shows strong clinical overlap with the brittle bone disease osteogenesis imperfecta (OI). BS is caused by biallelic mutations in either the FKBP10 or the PLOD2 gene. PLOD2 encodes the lysyl hydroxylase 2 (LH2) enzyme, which is responsible for the hydroxylation of lysine residues in fibrillar collagen telopeptides. This hydroxylation directs crosslinking of collagen fibrils in the extracellular matrix, which is necessary to provide stability and tensile integrity to the collagen fibrils. To further elucidate the function of LH2 in vertebrate skeletal development, we created a zebrafish model harboring a homozygous plod2 nonsense mutation resulting in reduced telopeptide hydroxylation and crosslinking of bone type I collagen. Adult plod2 mutants present with a shortened body axis and severe skeletal abnormalities with evidence of bone fragility and fractures. The vertebral column of plod2 mutants is short and scoliotic with compressed vertebrae that show excessive bone formation at the vertebral end plates, and increased tissue mineral density in the vertebral centra. The muscle fibers of mutant zebrafish have a reduced diameter near the horizontal myoseptum. The endomysium, a layer of connective tissue ensheathing the individual muscle fibers, is enlarged. Transmission electron microscopy of mutant vertebral bone shows type I collagen fibrils that are less organized with loss of the typical plywood‐like structure. In conclusion, plod2 mutant zebrafish show molecular and tissue abnormalities in the musculoskeletal system that are concordant with clinical findings in BS patients. Therefore, the plod2 zebrafish mutant is a promising model for the elucidation of the underlying pathogenetic mechanisms leading to BS and the development of novel therapeutic avenues in this syndrome. © 2016 American Society for Bone and Mineral Research.
Efficient heart disease prediction system using optimization technique

In this modern society where large number of humans follow a sedentary lifestyle following an 8 hour job cycle, cardio vascular diseases or heart diseases is one of the leading causes of mortality worldwide. The computers at the hospitals of the healthcare industries are used to collect huge amounts of information regarding the patients and their ailments. This huge repository of information contains wealth of knowledge. The hidden patterns and relationships in the data is mostly overlooked. Diagnosing cardio vascular diseases in patients is a difficult task and doctors who can accurately predict such diseases are few in number. This research paper focuses on developing a prediction algorithm with the help of data mining and optimization techniques. Data Mining refers to using a variety of techniques to identify information or decision making knowledge in the database and extracting these in a way that they can put to use in areas such as decision support, predictions, forecasting and estimation. We will be using the Particle Swarm Optimization technique which is an inherently distributed algorithm where the solution for a problem emerges from the interactions between many simple individual agents called particles. The data source we have used for experimental testing are commonly used and considered as a de facto standard for heart disease prediction reliability ranking. We will also be using a slightly modified version of PSO with constriction factor called Constricted PSO. The results obtained show that Particle Swarm Data Mining Algorithms are competitive, not only with other evolutionary techniques, but also with industry standard algorithms, and can be successfully applied to heart disease prediction.
Local Burn Injury Impairs Epithelial Permeability and Antimicrobial Peptide Barrier Function in Distal Unburned Skin*

Objectives:Our objective was to characterize the mechanisms by which local burn injury compromises epithelial barrier function in burn margin, containing the elements necessary for healing of the burn site, and in distal unburned skin, which serves as potential donor tissue. Design:Experimental mouse scald burn injury. Setting:University Research Laboratory. Subjects:C57/Bl6 Male mice, 8–12 weeks old. Interventions:To confirm that dehydration was not contributing to our observed barrier defects, in some experiments mice received 1 mL of saline fluid immediately after burn, while a subgroup received an additional 0.5 mL at 4 hours and 1 mL at 24 hours following burn. We then assessed skin pH and transepidermal water loss every 12 hours on the burn wounds for 72 hours postburn. Measurements and Main Results:Burn margin exhibited increased epidermal barrier permeability indicated by higher pH, greater transepidermal water loss, and reduced lipid synthesis enzyme expression and structural protein production up to 96 hours postburn. By contrast, antimicrobial peptide production and protease activity were elevated in burn margin. Skin extracts from burn margin did not exhibit changes in the ability to inhibit bacterial growth. However, distal unburned skin from burned mice also demonstrated an impaired response to barrier disruption, indicated by elevated transepidermal water loss and reduced lipid synthesis enzyme and structural protein expression up to 96 hours postburn. Furthermore, skin extracts from distal unburned skin exhibited greater protease activity and a reduced capacity to inhibit bacterial growth of several skin pathogens. Finally, we established that antimicrobial peptide levels were also altered in the lung and bladder, which are common sites of secondary infection in burn-injured patients. Conclusions:These findings reveal several undefined deficiencies in epithelial barrier function at the burn margin, potential donor skin sites, and organs susceptible to secondary infection. These functional and biochemical data provide novel insights into the mechanisms for graft failure and secondary infection after burn injury.
When students' self regulation of learning in peer group encounters novel knowledge at university

Collaborating the perspectives of socialization and self regulation of learning in peer group, the research examines the practical mechanism of students' self regulation in learning at university. Individuals' self regulation in learning and the influence from the ambient of peer group were explored through the lens of regulation in different aspects of individuals' metacognition, which are monitoring and self-regulation. Based on specific background of course learning, representing students' transacting from pre-tertiary courses to professional degree courses, the findings of research showed that deficiency in knowledge at level of metacognition limited students' abilities of self regulation in learning when facing with novel and high level knowledge. It might be the blind spot of present higher education and its impaction might be extending and sprawling.