Miracle-dz/UniFAR-FTUs huggingface dataset:
The CreA Repressor Is the Sole DNA-binding Protein Responsible for Carbon Catabolite Repression of the alcA Gene inAspergillus nidulans via Its Binding to a Couple of Specific Sites*

Carbon catabolite repression is mediated inAspergillus nidulans by the negative acting protein CreA. The CreA repressor plays a major role in the control of the expression of the alc regulon, encoding proteins required for the ethanol utilization pathway. It represses directly, at the transcriptional level, the specific transacting gene alcR, the two structural genes alcA and aldA, and other alc genes in all physiological growth conditions. Among the seven putative CreA sites identified in the alcApromoter region, we have determined the CreA functional targets in AlcR constitutive and derepressed genetic backgrounds. Two different divergent CreA sites, of which one overlaps a functional AlcR inverted repeat site, are largely responsible for alcA repression. Totally derepressed alcA expression is achieved when these two CreA sites are disrupted in addition to another single site, which overlaps the functional palindromic induction target. The fact that derepression is always associated with alcA overexpression is consistent with a competition model between AlcR and CreA for their cognate targets in the same region of the alcApromoter. Our results also indicate that the CreA repressor is necessary and sufficient for the total repression of the alcA gene.
γ-Amino butyric acid (GABA) release in the ciliated protozoon Paramecium occurs by neuronal-like exocytosis

SUMMARY Paramecium primaurelia expresses a significant amount of γ-amino butyric acid (GABA). Paramecia possess both glutamate decarboxylase (GAD)-like and vesicular GABA transporter (vGAT)-like proteins, indicating the ability to synthesize GABA from glutamate and to transport GABA into vesicles. Using antibodies raised against mammalian GAD and vGAT, bands with an apparent molecular weight of about 67 kDa and 57 kDa were detected. The presence of these bands indicated a similarity between the proteins in Paramecium and in mammals. VAMP, syntaxin and SNAP, putative proteins of the release machinery that form the so-called SNARE complex, are present in Paramecium. Most VAMP, syntaxin and SNAP fluorescence is localized in spots that vary in size and density and are primarily distributed near the plasma membrane. Antibodies raised against mammal VAMP-3, sintaxin-1 or SNAP-25 revealed protein immunoblot bands having molecular weights consistent with those observed in mammals. Moreover, P. primaurelia spontaneously releases GABA into the environment, and this neurotransmitter release significantly increases after membrane depolarization. The depolarization-induced GABA release was strongly reduced not only in the absence of extracellular Ca2+ but also by pre-incubation with bafilomycin A1 or with botulinum toxin C1 serotype. It can be concluded that GABA occurs in Paramecium, where it is probably stored in vesicles capable of fusion with the cell membrane; accordingly, GABA can be released from Paramecium by stimulus-induced, neuronal-like exocytotic mechanisms.
Concentration of rafts in platelet filopodia correlates with recruitment of c‐Src and CD63 to these domains

Summary. The molecular mechanism that causes non‐adhesive, discoid platelets to transform into sticky dendritic bodies that form blood clumps is a complex series of events. Recently it has become clear that lipid microdomains—also known as rafts—play a crucial role in this process. We have used a non‐cytolytic derivative of perfringolysin‐O, a cholesterol binding cytolysin, that binds selectively to cholesterol‐rich membrane domains, combined with confocal‐ and immunoelectron microscopy to visualize cholesterol‐raft dynamics during platelet adhesion. In resting platelets cholesterol was uniformly distributed on the cell surface and confined to distinct intracellular compartments (i.e. multivesicular bodies, dense granules, and the internal membranes of α‐granules). Upon interaction with fibrinogen, cholesterol accumulated at the tips of filopodia and at the leading edge of spreading cells. Stimulation with thrombin receptor activating peptide (TRAP) resulted in a similar redistribution of cholesterol towards filopodia. The adhesion‐dependent raft aggregation was accompanied by concentration of the tyrosine kinase c‐Src and the tetraspanin CD63 in these domains, whereas glycoprotein Ib (GPIb) was not selectively targeted to the raft clusters. c‐Src, the tetraspanin CD63, and GPIb were recovered in biochemically isolated low‐density membrane fractions. Disruption of rafts by depleting membrane cholesterol had no effect on platelet shape change but inhibited platelet spreading on fibrinogen and TRAP‐induced aggregation. Our results demonstrate that cholesterol rafts in platelets are dynamic entities in the membrane that co‐cluster with the tyrosine kinase c‐Src and the costimulatory molecule CD63 in specialized domains at the cell surface, thereby providing a possible mechanism in functioning as signaling centres.
Transient growth in Taylor–Couette flow

Transient growth due to non-normality is investigated for the Taylor–Couette problem with counter-rotating cylinders as a function of aspect ratio η and Reynolds number Re. For all Re⩽500, transient growth is enhanced by curvature, i.e., is greater for η 130 the greatest transient growth is achieved for η on the linear stability boundary. Transient growth is shown to be approximately 20% higher near the linear stability boundary at Re=310, η=0.986 than at Re=310, η=1, near the threshold observed for transition in plane Couette flow. The energy in the optimal inputs is primarily meridional; that in the optimal outputs is primarily azimuthal. Pseudospectra are calculated for two contrasting cases. For large curvature, η=0.5, the pseudospectra adhere more closely to the spectrum than in a narrow gap.
On Fixed Points of the Burrows-Wheeler Transform

The Burrows-Wheeler Transform is a well known transformation widely used in Data Compression: important competitive compression software, such as Bzip (cf. [16]) and Szip (cf, [15]) and some indexing software, like the FM-index (cf. [4]), are deeply based on the Burrows Wheeler Transform. The main advantage of using BWT for data compression consists in its feature of “clustering” together equal characters. In this paper we show the existence of ﬁxed points of BWT, i
Safe feeding strategies for a physically assistive robot

With aging societies and the increase of handicapped people the demand for robots that can help nursing humans on-site is increasing. Concretely, according to World Health Organization (WHO) by 2030 more than 2 billion people will need one or more assistive products. With this perspective it becomes vital to develop assistive technology products as they maintain or improve disabled people’s functioning and independence. One of the most important activities that a person needs to be able to perform in order to feel independent is self-feeding. The main objective of this thesis is to develop software that controls a robot in order to feed a disabled person autonomously. Special attention has been given to the safety and naturalness of the task performance. The resulting system has been tested in the Barrett WAM® robot. In order to fulfill this goal an RGB-D camera has been used to detect the head orientation and the state of the mouth. The first detection has been realized with the OpenFace library whereas the second one has been realized with the OpenPose library. Finally, the depth obtained by the camera has been used to identify and cope with wrong detections. Safety is an essential part of this thesis as it exists direct contact between the user and the robot. Therefore, the feeding task must be completely safe for the user. In order to achieve this safety two di˙erent types of security have been considered: passive safety and active safety. The passive safety is achieved with the compliance of the robot whereas active safety is achieved limiting the maximum force that is obtained with a force sensor. Some experiments have been carried out to determine which is the best setup for the robot to ensure a safe task performance. The designed system is capable of automatically detecting head orientation and mouth state and decide which action to take at any moment given this information. It is also capable of stopping the robot movement when certain forces are reached, return to the previous position and wait in this position until it is safe to perform that action again. A set of experiments with healthy users has been carried out to validate the proposed system and the results are presented here.
Antimicrobial activity of a mixture of two isomeric phenylpropanoid glycosides from Arrabidaea harleyi A.H. Gentry (Bignoniaceae)

Arrabidaea harleyi A.H. Gentry (Bignoniaceae) e uma planta ornamental, encontrada em algumas regioes da Mata Atlântica do Brasil. A partir das cascas do caule foi isolada a mistura dos isomeros verbascosideo e isoverbascosideo. A mistura mostrou-se ativa frente a Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Bacillus mycoides, Enterococcus faecalis, Escherichia coli, Serratia marcensis e Candida albicans. Foi estabelecida a concentracao minima inibitoria (CMI) atraves do metodo de difusao em meio solido.
Arbitration and Sharing Control Strategies in the Driving Process

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Arbitration and Sharing Control Strategies in the Driving Process David González, Joshué Pérez, Vicente Milanés, Fawzi Nashashibi, Marga Saez Tort, Angel Cuevas
The evaluation system in facial recognize algorithm research

This system is needed to make the designing and research of algorithm more efficient. Two aspects are adopted in the designing of the system, the first is software designing and the second is the construction of image database with complex background. The software adopts several modules in the designing which is convenient for updating. Batch processing function is put into this software to simplify the evaluation procedure. Video database and image database with complex background are added into the system, that makes the system more robust. Through the evaluation of eye locating algorithm, the batch processing function is well proved and the system can record the run time of the algorithm, that makes the research process much more efficiency.
Validating a Scale of Health Beliefs in Preventive Health Screenings Among Chinese Older Adults

We aimed to examine the psychometric properties of a modified 16-item Attitudinal Index (AI), a measure of Chinese older adults’ beliefs about preventive health screenings. We used the 2013 Shanghai Elderly Life and Opinion data including 3,418 respondents age 60+ who were randomly split into training and validation samples. We examined the validity and reliability of the modified AI. Psychometric evaluation of the modified AI revealed good response patterns. The overall scale had good reliability (Cronbach’s α = .835). Exploratory factor analysis yielded four factors: barriers, fatalism, unnecessary, and detects (Cronbach’s α = .815-.908). Confirmatory factor analysis of the modified AI’s factor structure verified its four-factor structure (comparative fit index = 0.913, standardized root mean square residual = 0.048). The validity and reliability of the modified AI support its cultural appropriateness in measuring health beliefs among Chinese elderly. Further psychometric evaluation should focus on testing concurrent and criterion validity.
RGC-5 cells.

Our laboratory previously reported the generation of a retinal ganglion cell 5 (RGC-5) cell line with phenotypic expression of many retinal ganglion cell markers.1 The RGC-5 cell line has been widely used since then, reproducing several of the original findings and their retinal ganglion cell phenotype.2–8 ISI Web of knowledge gives 260 references by using “RGC-5” as a search word under topic. However, last month the first author of the original study1 published a report9 that, along with previous studies,10,11 stated that the RGC-5 line is the same as 661W, a cell line believed to be of cone photoreceptor origin.12 As the two cell types are very different by molecular phenotyping, it is difficult to reconcile this discrepancy. In fact, the same group who published this recent report9 previously used RGC-5 cells for other studies, confirming their retinal ganglion cell origin (Dauphin et al. IOVS 2003;44:ARVO E-Abstract 2249; Krishnamoorthy et al. IOVS 2005;46:ARVO E-Astract 2215; and Refs. 13 and 14). Furthermore, the originator of 661W cells and many others used RGC-5 cells in several published studies15–18 and reported different phenotypic properties between RGC-5 and 661W cell types. So, what is the true phenotype of 661W cells? Interestingly, the two coauthors of Krishnamoorthy et al.9 published a paper on sigma-1 receptor (sigma-1r) in purified retinal ganglion cells,19 in which they corroborated their coimmunoprecipitation data of sigma-1r with L-type volted gated calcium channels in purified RGCs with that of RGC-513 and suggested, “Our co-localization data in purified RGCs is in agreement with the above studies” done with RGC-5 cells. Furthermore, there were two editorials on authentication of RGC-5 cells,20,21 suggesting RGC-5 may have never existed. These editorials have totally ignored the vast published literature on their ganglion cells' origin even by some of the coeditors' own studies. As I have been without an active laboratory for the last several years, I am in no position to validate the published reports. In light of these revelations from the first author of the original study who could not reproduce his own work, it became imperative for me to dissociate myself from the original work and, as a corresponding author, have requested that the original manuscript be retracted from the Molecular Brain Research journal.1 I sincerely regret this incidence and apologize to my colleagues in vision research—and I agree that one has to be very careful while using cultured cells in research.
Common functions of central and posterior Hox genes for the repression of head in the trunk of Drosophila

Hox genes are localised in complexes, encode conserved homeodomain transcription factors and have mostly been studied for their specialised functions: the formation of distinct structures along the anteroposterior axis. They probably derived via duplication followed by divergence, from a unique gene, suggesting that Hox genes may have retained a common function. The comparison of their homeodomain sequences groups Hox proteins into Anterior, Central and Posterior classes, reflecting their expression patterns in the head, trunk and tail, respectively. However, functional data supporting this classification are rare. Here, we re-examine a common activity of Hox genes in Drosophila: the repression of head in the trunk. First, we show that central and posterior Hox genes prevent the expression of the head specific gene optix in the trunk, providing a functional basis for the classification. Loss-of-function mutations of optix affect embryonic head development, whereas ectopic Optix expression strongly perturbs trunk development. Second, we demonstrate that the non-Hox genes teashirt, extradenticle and homothorax are required for the repression of optix and that Wingless signalling and Engrailed contribute to this repression. We propose that an evolutionary early function of Hox genes was to modify primitive head morphology with novel functions specialising the trunk appearing later on.
Gradual Retirement in the Netherlands

Gradual retirement by which individuals leave their career jobs and withdraw incrementally from the labor force is an important empirical phenomenon in the United States. We analyze the current state of gradual retirement in the Netherlands using administrative data that allow much more precise tracking of labor market transitions than most survey panel data. We estimate multinomial transition models, taking into account competing pathways out of career employment at older ages, and discuss institutional aspects that limit the scope of gradual retirement, such as financial incentives to retire early.
Comparison of Clutter and Multipath Cancellation Techniques for Passive Radar

In this paper we address the problem of clutter and multipath cancellation in passive radar. Different adaptive techniques are considered based on both well known approaches and innovative strategies proposed by the authors. The performance comparison allows to understand the particular behaviour of each technique in a practical scenario. The proposed innovative strategies are shown to be very appealing solutions for disturbance cancellation in passive radar.
Prédiction des buzz sur Twitter

RESUME. La prediction des buzz sur Internet est une tâche difficile notamment parce que le phe-nomene est dependant de parametres tres divers, lies au contenu du message lui-meme mais aussi au contexte de sa diffusion et a la dynamique de propagation de l'information sur la toile. Ces difficultes se trouvent augmentees par la dimension du Web et la dispersion et la fragmentation des informations qui s'y trouvent. Twitter est un espace d'experimentation plus contraint et delimite que le Web dans sa globalite; dans cet article, nous presentons une methode de prediction des buzz appliquee a la prediction des pics de re-emissions (retweets) des messages postes sur Twitter. La methode proposee repose essentiellement sur trois types d'indicateurs dont nous pensons qu'ils participent a la probabilite de re-diffusion d'un tweet : la popula-rite, la saillance thematique et l'expressivite. Ces descripteurs sont utilises comme variables d'entree d'un reseau de neurones dont le role est de predire le depassement d'un seuil de re-emission du message. Les tests, conduits sur un ensemble d'environ 30000 messages, montrent l'efficacite de l'approche proposee : le systeme detecte plus de 72% des messages re-diffuses au moins 60 fois. ABSTRACT. The prediction of bursty events on the internet is a challenging task. Difficulties are due to the diversity of information sources, the size of the internet, dynamics of popularity, user behaviors. On the other hand, Twitter is a structured and limited space. In this paper, we present a bursty event prediction method applied to the Twitter plateform. The proposed method uses tweet contents to predict the retweet rate. Prediction system extracts 3 types of features, related respectively to popularity, saliance and expressivity. These descriptors constitute the input features of a multilayer perceptron that predicts the retweet rate. Our experiments are conducted on a test corpus composed by about 30,000 tweets. On this test set, the proposed system detects more than 72% of the tweets that have been forwarded at least 60 times.
Developing graphene based MMICs on SiC substrate

This paper presents the fabrication process development of monolithic microwave integrated circuits (MMICs) based on graphene field effect transistors (G-FETs). In this process, the G-FETs are based on epitaxial graphene on SiC substrate. Base on this process, a wide-band amplifier (0-3GHz) with 6-7 dB gain is realized.
Prevalencia de fasciolosis bovina en una zona de Caldas Colombia con evidencias de la enfermedad

La fasciolosis es una parasitosis que afecta rumiantes, equinos, porcinos, entre otras especies y es causante de grandes perdidas economicas y puede parasitar humanos. En la presente investigacion, se evaluo la frecuencia de la enfermedad por tres metodos de deteccion, en veintiseis predios, localizados en los municipios del departamento de Caldas, con mayor prevalencia, segun reportes de la Central de beneficio de Manizales. Se utilizaron los metodos de coprologia, PCR y ELISA, en heces y en suero de bovinos seleccionados aleatoriamente en predios, con el sistema de produccion de leche bovina, localizados en los Andes colombianos, entre 1.980-3.721 msnm. Se obtuvieron 414 muestras de materia fecal, procesadas con la tecnica Dennis modificada. El ADN fue extraido de las heces usando un kit comercial y fue amplificado con cebadores especificos, por PCR convencional. En las muestras de suero, se detecto la presencia de inmunoglobulina G (IgG) contra Fasciola hepatica , a traves del uso de un Kit comercial, especifico para este fin. La region estudiada, se dividio en cuatro zonas, para efectos del estudio. La prevalencia de la fasciolosis por coprologia fue 12,3%; por Elisa, 19,1% y por PCR, 67,2%. La zona tres presento la mayor proporcion de animales positivos, por los tres metodos, con odds ratio elevados y alta significancia estadistica; fueron hallados caracoles Limneidos en ambientes acuaticos, aptos para su desarrollo, que permiten perpetuar el inoculo. Se confirma la presencia de fasciolosis bovina en la region estudiada y se requieren hacer estudios adicionales, para validar la PCR, como metodo para la deteccion de fasciolosis.
Cuts, consistency statements and interpretations

Interpretability in reflexive theories, especially in PA, has been studied in many papers; see e.g. [3], [6], [7], [10], [11], [15], [26]. It has been shown that reflexive theories exhibit many nice properties, e.g. (1) if T, S are recursively enumerable reflexive, then T is interpretable in S iff every Π1 sentence provable in T is provable in S; and (2) if S is reflexive, T is recursively enumerable and locally interpretable in S (i.e. every finite part of T is interpretable in S), then T is globally interpretable in S (Orey's theorem, cf. [3]). In this paper we want to study such statements for nonreflexive theories, especially for finitely axiomatizable theories (which are never reflexive). These theories behave differently, although they may be quite close to reflexive theories, as e.g. GB to ZF. An important fact is that in such theories one can define proper cuts. By a cut we mean a formula with one free variable which defines a nonempty initial segment of natural numbers closed under the successor function. The importance of cuts for interpretations in GB was realized already by Vopěnka and Hájek in [30]. Pioneering work was done by Solovay in [24]. There he developed the method of “shortening of cuts”. Using this method it is possible to replace any cut by a cut which is contained in it and has some desirable additional properties; in particular it can be closed under + and ·. This introduces ambiguity in the concept of arithmetic in theories which admit proper cuts, namely, which cut (closed under + and ·) should be called the arithmetic of the theory? Cuts played the crucial role also in [20].