Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic Selection of Peptide Aptamers That Interact and Inhibit Both Small Protein B and Alternative Ribosome-Rescue Factor A of Aeromonas veronii C4

Aeromonas veronii is a pathogenic gram-negative bacterium, which infects a variety of animals and results in mass mortality. The stalled-ribosome rescues are reported to ensure viability and virulence under stress conditions, of which primarily include trans-translation and alternative ribosome-rescue factor A (ArfA) in A. veronii. For identification of specific peptides that interact and inhibit the stalled-ribosome rescues, peptide aptamer library (pTRG-SN-peptides) was constructed using pTRG as vector and Staphylococcus aureus nuclease (SN) as scaffold protein, in which 16 random amino acids were introduced to form an exposed surface loop. In the meantime both Small Protein B (SmpB) which acts as one of the key components in trans-translation, and ArfA were inserted to pBT to constitute pBT-SmpB and pBT-ArfA, respectively. The peptide aptamer PA-2 was selected from pTRG-SN-peptides by bacterial two-hybrid system (B2H) employing pBT-SmpB or pBT-ArfA as baits. The conserved sites G133K134 and D138K139R140 of C-terminal SmpB were identified by interacting with N-terminal SN, and concurrently the residue K62 of ArfA was recognized by interacting with the surface loop of the specific peptide aptamer PA-2. The expression plasmids pN-SN or pN-PA-2, which combined the duplication origin of pRE112 with the neokanamycin promoter expressing SN or PA-2, were created and transformed into A. veronii C4, separately. The engineered A. veronii C4 which endowing SN or PA-2 expression impaired growth capabilities under stress conditions including temperatures, sucrose, glucose, potassium chloride (KCl) and antibiotics, and the stress-related genes rpoS and nhaP were down-regulated significantly by Quantitative Real-time PCR (qRT-PCR) when treating in 2.0% KCl. Thus, the engineered A. veronii C4 conferring PA-2 expression might be potentially attenuated vaccine, and also the peptide aptamer PA-2 could develop as anti-microbial drugs targeted to the ribosome rescued factors in A. veronii.
Is Epipolar Geometry Necessary ?

We examine and contrast the projective properties of two simple 3D configurations. The first consists of six points, four of which are coplanar. We prove that epipolar geometry and the essential matrix can be recovered uniquely for this structure and give a constructive algorithm for this. The second configuration has four coplanar points and a single non-coplanar line. In this case it is not possible to determine the epipolar geometry. However, both structures have two projective invariants, and these are recoverable from two (uncalibrated) perspective images. We include examples of the invariants for real objects.
Hybrid Indexes to Expedite Spatial-Visual Search

Due to the growth of geo-tagged images, recent web and mobile applications provide search capabilities for images that are similar to a given query image and simultaneously within a given geographical area. In this paper, we focus on designing index structures to expedite these spatial-visual searches. We start by baseline indexes that are straightforward extensions of the current popular spatial (R*-tree) and visual (LSH) index structures. Subsequently, we propose hybrid index structures that evaluate both spatial and visual features in tandem. The unique challenge of this type of query is that there are inaccuracies in both spatial and visual features. Therefore, different traversals of the index structures may produce different images as output, some of which more relevant to the query than the others. We compare our hybrid structures with a set of baseline indexes in both performance and result accuracy using three real world datasets from Flickr, Google Street View, and GeoUGV.
SLAM in large-scale cyclic environments using the Atlas framework

This paper describes Atlas, a hybrid metrical/topological approach to simultaneous localization and mapping (SLAM) that achieves efficient mapping of large-scale environments. The representation is a graph of coordinate frames, with each vertex in the graph representing a local frame and each edge representing the transformation between adjacent frames. In each frame, we build a map that captures the local environment and the current robot pose along with the uncertainties of each. Each map’s uncertainties are modeled with respect to its own frame. Probabilities of entities with respect to arbitrary frames are generated by following a path formed by the edges between adjacent frames, computed using either Dijkstra’s shortest path algorithm or breath-first search. Loop closing is achieved via an efficient map matching algorithm coupled with a cycle verification step. We demonstrate the performance of the technique for post-processing large data sets, including an indoor structured environment (2.2 km path length) with multiple nested loops using laser or ultrasonic ranging sensors.
Biblioteca 2.0: levantamento do seu uso em bibliotecas

La presente investigacion examino y describio el uso de las herramientas y las tecnologias para la colaboracion, aplicadas a los servicios bibliotecarios 2.0. Para este fin se utilizo la investigacion exploratoria y descriptiva con ayuda de la investigacion bibliografica y el inventario con acercamiento cualicuantitativo. Para recopilar los datos el investigador aplico un formulario basado en el metodo de observacion en bibliotecas de los cinco continentes (muestra n = 21). Se constato mayor incidencia de herramientas y tecnologias online 2.0 en las bibliotecas universitarias en los servicios de Referencia, DSI y Atencion al usuario y las herramientas mas utilizadas fueron RSS, Blogs y Chats. En la categorizacion propuesta por Good (2007) se percibe mayor incidencia de las herramientas Mensajes Instantaneos y Publicacion y Participacion en la Web en las bibliotecas universitarias en los servicios de Referencia e Informacion y Feedback. Los resultados obtenidos permitieron observar que hay un numero considerable de estos tipos de herramientas en los servicios virtuales sincronicos y asincronicos y aunque hay diferencias en el tipo de interaccion hombremaquina, cada dia se percibe, sin embargo, el incremento de las ofertas de servicios virtuales para los usuarios academicos
Expression of multidrug resistance proteins is localized principally to the Malpighian tubules in larvae of the cabbage looper moth, Trichoplusia ni

The multidrug resistance proteins (MRPs) serve a number of important roles in development, physiological homeostasis and metabolic resistance. In insects, they may also contribute to resistance against xenobiotics including insecticides and plant secondary metabolites. To investigate their contribution to xenobiotic resistance, we have examined the tissue distribution of gene and protein expression of the multidrug resistance proteins TrnMRP1 and TrnMRP4 of the lepidopteran insect, Trichoplusia ni. Using quantitative PCR and immunohistochemistry, we have identified high expression levels of both transporters in the Malpighian tubules relative to levels in other major tissues of the body, where they probably contribute to excretion of metabolic wastes or ingested xenobiotics. We have specifically located TrnMRP protein expression in a subpopulation of Malpighian tubule secondary cells. Expression of TrnMRP1 was also detected both at a high level in specific cortical neurons of larval ganglia and at a lower level throughout the cortex, where it may act in signaling or protective functions, respectively. In contrast, expression of TrnMRP4 was low to absent in larval ganglia, with the exception of single cells in the central connective. We discuss the potential implications of this TrnMRP activity on insect development and metabolic resistance.
Distance Education: Learning Outcomes, Interaction, and Attitudes

This study examines the impact of telecommunications media upon learning outcomes designed on level of learning and instructional strategy. Course objectives were identified by type of learning using a model based upon Gagne and Briggs. A nonequivalent control group design was used to measure differences in learning outcomes. The data gathering techniques include content analyses of written exams, observations, interviews, an analysis of interactions, and examination of student attitudes using a questionnaire. Analysis of covariance measured differences between sites, controlling for entering knowledge as measured by the pretext. These analyses showed no significant differences on six of the evaluations.
A Novel 2-D Multibeam Antenna Without Beamforming Network

A novel design of multibeam array antenna without feeding network is presented in this communication. This array antenna consists of 3×3 microstrip patches as radiators. In this design, a feeding network is avoided where each patch is fed by a probe. Furthermore, whatever patch is excited, the input power can be coupled to all patches through four microstrip lines located between the radiating elements. In addition, nine radiation beams can be implemented depending on different field distributions that are generated by exciting each patch individually. The proposed antenna has a simple single-layered structure and does not suffer from a complex feeding network compared with traditional multibeam antennas. The experimental results demonstrate that the scanning ranges of the nine beams are ±24° and ±45° in the vertical and horizontal directions, respectively. Moreover, measured gain for the nine beams of the implemented antenna varies from 9.06 to 10.45 dBi.
Growth at Adolescence

Obese Children. By FLEMMING QUAADE. (Pp. 302. No price given.) Printed by Danish Science Press Ltd., Copenhagen. 1955. This book is the expansion of a prize essay on the subject of obesity in childhood, with special reference to Hilde Bruch's theory on the causation of this condition. Bruch supposed that an ambivalance exists in the attitudes of mothers to their obese children; that they have fundamentally hostile feelings compensated for by more obvious, but more superficial, over-protection and demonstrations of love, manifested by gifts of food. The book make a careful study of 185 obese children, analysing a variety of data and comparing them with similar data from over 1,000 non-obese children. Dr. Quaade eventually comes to two principal conclusions. First, that almost nothing distinguishes obese children from their slimmer friends other than their fatness, together with a characteristic emotional outlook which is not causative but a result of the impact their obesity makes upon their school friends. He concludes that there is little support of Dr. Bruch's thesis regarding the relationship of parents to their obese children. Secondly, he concludes that the main reason why children become fat is because of their excessive appetites, though he bases his opinions on impressions only. Dr. Quaade has nothing to offer regarding why some children's appetites lead them to eat more food and lay down more fat than others, and this surely is the crux of the problem which Dr. Bruch made an attempt to solve. The book is well printed, and though paper backed, is adequately bound. It abounds in tables and charts, and is a useful summary, without making additions to our knowledge, of the statistical facts regarding obesity.
Constraint-Driven Dynamic Workflow for Automation of Big Data Analytics Based on GraphPlan

The use of the big data analytics (BDA) platform is increasingly becoming prevalent in the data sciences. However, BDA processes consume resources and time excessively. Automating BDA processes is a cognitive approach to the BDA domain, which is most impaired by its heavy consumption of time and resources. However, the BDA workflow is highly dependent on diversified constraints because of the high variability, veracity, and volume of data processing to accomplish highly influential and sophisticated requirements. The workflow has to pass rigorous and diverse data mining steps, each step contains several tasks and these tasks made by many sub tasks, that it must be accomplished to progress to the next step in the workflow. This increases the available solution space for BDA processes. The intelligent heuristic approach is needed to address the domain-specific concerns which are large solution space, and awareness of constraints are caused the BDA planning. Therefore, we propose to use GraphPlan-based dynamic workflow generation for the BDA domain. Our empirical studies prove that the proposed sophisticated method satisfied planning requirements and outperformed a related planning technique.
Innumeracy: Mathematical Illiteracy and Its Consequences

Why do even well-educated people often understand so little about maths - or take a perverse pride in not being a 'numbers person'? In his now-classic book "Innumeracy", John Allen Paulos answers questions such as: why is following the stock market exactly like flipping a coin; how big is a trillion; how fast does human hair grow in mph; and, can you calculate the chances that a party includes two people who have the same birthday. Paulos shows us that by arming yourself with some simple maths, you don't have to let numbers get the better of you.
Solution 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin

19F nuclear Overhauser effects (NOEs) between fluorine labels on the cytoplasmic domain of rhodopsin solubilized in detergent micelles are reported. Previously, high-resolution solution 19F NMR spectra of fluorine-labeled rhodopsin in detergent micelles were described, demonstrating the applicability of this technique to studies of tertiary structure in the cytoplasmic domain. To quantitate tertiary contacts we have applied a transient one-dimensional difference NOE solution 19F NMR experiment to this system, permitting assessment of proximities between fluorine labels specifically incorporated into different regions of the cytoplasmic face. Three dicysteine substitution mutants (Cys-140–Cys-316, Cys-65–Cys-316, and Cys-139–Cys-251) were labeled by attachment of the trifluoroethylthio group through a disulfide linkage. Each mutant rhodopsin was prepared (8–10 mg) in dodecylmaltoside and analyzed at 20°C by solution 19F NMR. Distinct chemical shifts were observed for all of the rhodopsin 19F labels in the dark. An up-field shift of the Cys-316 resonance in the Cys-65–Cys-316 mutant suggests a close proximity between the two residues. When analyzed for 19F-19F NOEs, a moderate negative enhancement was observed for the Cys-65–Cys-316 pair and a strong negative enhancement was observed for the Cys-139–Cys-251 pair, indicating proximity between these sites. No NOE enhancement was observed for the Cys-140–Cys-316 pair. These NOE effects demonstrate a solution 19F NMR method for analysis of tertiary contacts in high molecular weight proteins, including membrane proteins.
BDDStab : BDD-based Value Analysis of Binaries

Value analyses compute for each variable a superset of possible values, called variation domain (VD). The results of value analyses are helpful for verification as well as program comprehension and are often used to enable or improve other analyses. For highlevel languages like C, value analyses are utilized, for example, to compute targets of dynamic call sites. For executables, value analyses could be similarly useful, for example, for control-flow reconstruction. Compared to value analyses on high-level languages, the analysis of executables poses the following challenges:
Age-Related Differences in Advantageous Decision Making Are Associated with Distinct Differences in Functional Community Structure

Human decision making is dependent on not only the function of several brain regions but also their synergistic interaction. The specific function of brain areas within the ventromedial prefrontal cortex has long been studied in an effort to understand choice evaluation and decision making. These data specifically focus on whole-brain functional interconnectivity using the principles of network science. The Iowa Gambling Task (IGT) was the first neuropsychological task used to model real-life decisions in a way that factors reward, punishment, and uncertainty. Clinically, it has been used to detect decision-making impairments characteristic of patients with prefrontal cortex lesions. Here, we used performance on repeated blocks of the IGT as a behavioral measure of advantageous and disadvantageous decision making in young and mature adults. Both adult groups performed poorly by predominately making disadvantageous selections in the beginning stages of the task. In later phases of the task, young adults shifted to more advantageous selections and outperformed mature adults. Modularity analysis revealed stark underlying differences in visual, sensorimotor and medial prefrontal cortex community structure. In addition, changes in orbitofrontal cortex connectivity predicted behavioral deficits in IGT performance. Contrasts were driven by a difference in age but may also prove relevant to neuropsychiatric disorders associated with poor decision making, including the vulnerability to alcohol and/or drug addiction.
Improving the Visual Comprehension of Point Sets

Point sets are the standard output of many 3D scanning systems and depth cameras. Presenting the set of points as is, might "hide" the prominent features of the object from which the points are sampled. Our goal is to reduce the number of points in a point set, for improving the visual comprehension from a given viewpoint. This is done by controlling the density of the reduced point set, so as to create bright regions (low density) and dark regions (high density), producing an effect of shading. This data reduction is achieved by leveraging a limitation of a solution to the classical problem of determining visibility from a viewpoint. In addition, we introduce a new dual problem, for determining visibility of a point from infinity, and show how a limitation of its solution can be leveraged in a similar way.
Researching Lived Experience: Human Science for an Action Sensitive Pedagogy

Phenomenology as Qualitative ResearchHow to Write a Phenomenological DissertationPhenomenology of PracticePedagogical TactWriting in the DarkThe Prose of the WorldDoing Hermeneutic Phenomenological ResearchLived Experiences of MulticultureThe Lived Experience of Climate ChangeThe SAGE Encyclopedia of Qualitative Research MethodsResearching Lived ExperienceSymbolic Interaction and Ethnographic ResearchThe Tact of TeachingChildhood's SecretsRESEARCHING LIVED EXPERIENCE, HUMAN SCIENCE FOR AN AC.Succeeding with Your DoctorateEthnographic Inquiry and Lived ExperienceThe Nature of SympathyPhenomenological Research MethodsInterpretative Phenomenological AnalysisHeuristic InquiryCrafting Phenomenological ResearchPhenomenological PsychologyResearching Lived Experience, Second EditionCompleting Your Qualitative DissertationQualitative Research for the Social SciencesTransformative PhenomenologyInterpretive PhenomenologyThe Rustle of LanguageResearching Lived Experience, Second EditionPhenomenology of PerceptionHeuristic ResearchThe Structures of the Life-worldUnderstanding the Lived Experiences of Persons with Disabilities in Nine CountriesQualitative InquiryInvestigating SubjectivityPhenomenology and Human Science Research TodayResearching Lived Experience, Second EditionIntroduction to the Human SciencesSharing Qualitative Research
Masculinity and Femininity: The Taboo Dimension of National Cultures

Masculinity and Femininity is the first in-depth discussion of the masculinity dimension, and how it can help us to understand differences amongst cultures. Geert Hofstede begins with a general explanation of the masculinity dimension, and discusses how it illuminates broad features of different cultures. Parts Two, Three and Four apply the dimension more specifically to gender (and gender identity), sexuality (and sexual behaviour), and religion, probably the most influential variable of all. The book closes with a syntesizing statement about cultural values as they are linked to sexulaity, gender and religion.
A characterization of solutions for general copositive quadratic Lyapunov inequalities

This article provides answers to an open question raised in [1] with regard to checking existence of a solution for general copositive Lyapunov inequalities. We consider homogeneous LTI systems that preserve a proper cone C. * A necessary and sufficient condition for stability of such a system is the existence of a quadratic Lyapunov solution for the associated copositive Lyapunov inequality. This article provides a computationally efficient alternative necessary and sufficient condition for stability of the cone-invariant LTI system, in which geometric algebraic conditions for the stability of an equilibrium state are established from the concepts of dual and polar cones. The conditions are polynomial-time verifiable, provided C is a proper cone in a Hilbert space and has a polynomial-time evaluable self-concordant barrier function. We show that the feasible solutions of those conditions can be used to characterize the extreme rays of the set of solutions for copositive Lyapunov inequalities.
About how to capture and exploit the CO 2 surplus that nature, per se, is not capable of fixing

Human activity has been altering many ecological cycles for decades, disturbing the natural mechanisms which are responsible for re‐establishing the normal environmental balances. Probably, the most disrupted of these cycles is the cycle of carbon. In this context, many technologies have been developed for an efficient CO2 removal from the atmosphere. Once captured, it could be stored in large geological formations and other reservoirs like oceans. This strategy could present some environmental and economic problems. Alternately, CO2 can be transformed into carbonates or different added‐value products, such as biofuels and bioplastics, recycling CO2 from fossil fuel. Currently different methods are being studied in this field. We classified them into biological, inorganic and hybrid systems for CO2 transformation. To be environmentally compatible, they should be powered by renewable energy sources. Although hybrid systems are still incipient technologies, they have made great advances in the recent years. In this scenario, biotechnology is the spearhead of ambitious strategies to capture CO2 and reduce global warming.
Neuronal detection thresholds during vestibular compensation: contributions of response variability and sensory substitution

Unilateral vestibular injury impairs our ability to detect motion. However, before this study the neural mechanisms underlying this impairment had not yet been established. We found that the detection thresholds of neurons at the first central stage of vestibular processing (i.e. vestibular nuclei) dramatically increase immediately post‐lesion, and despite some recovery remain elevated even after 1 month, following the trend reported for vestibular patients’ perception. After the lesion, parallel changes in neuronal trial‐to‐trial variability and sensitivity account for consistently elevated thresholds, thus providing a neural correlate for impaired behavioural performance. In a subset of neurons, sensory substitution with extravestibular (i.e. proprioceptive) inputs after the lesion combined with residual vestibular information serves to improve neuronal detection thresholds for head‐on‐body motion. Our results provide a neural correlate for rehabilitation approaches that take advantage of the convergence of proprioceptive and vestibular inputs to improve patient outcomes.