Miracle-dz/UniFAR-FTUs huggingface dataset:
HIGH RESISTANCE/LOW REPETITION VS.LOW RESISTANCE/HIGH REPETITION TRAINING: EFFECTS ON PERFORMANCE OF TRAINED CYCLISTS

In order to investigate the effects of a resistance training modality on cycling performance, 23 trained club-level cyclists were placed into high resistance/low repetition (H-Res), low resistance/high repetition (H-Rep), or cycling-only groups for a 10-week program. All 3 groups followed the same cycling plan, but the H-Res and H-Rep groups added resistance training. Testing pre and post consisted of a graded incremental lactate profile test on an ergometer, with blood lactate being sampled. &OV0312;O2 values were measured to determine economy. Maximum strength testing of 4 strength exercises targeting the lower extremity musculature was conducted with the H-Res and H-Rep groups. There were significant gains in all 4 resistance training exercises (p = 0.05) for both H-Res and H-Rep, with the H-Res group having significantly greater gains than the H-Rep group had in the leg press exercise (p = 0.05). There were, however, no significant group × training differences (p < 0.05) found between the 3 training groups on the cycling test in lactate values or economy. It appears that for this population of cyclists, neither H-Res nor H-Rep resistance training provided any additional performance benefit in a graded incremental cycling test when compared with cycling alone over a training time of this length. It is possible that with this population, various factors such as acute fatigue, strength, and aerobic gains from the cycling training, in addition to well-developed bases of strength and conditioning from previous training, reduced differences between groups in both strength gains and cycling performance.
Energy efficiency of Unicast Support Multicast with QoS guarantee

Multicast transmission is known as an efficient mechanism for offering the same services for different terminals. However, the power consumption is surprisingly arisen due to terminals with the worst channel quality while maintaining the Quality of Service (QoS) and data decoding requirements. In order to address this problem, we examine the energy saving performance of an efficient algorithm called Unicast Support Multicast (USM), which can dynamically and expeditiously use unicast transmission as an additional support for multicast transmission. The USM algorithm can overcome the inherent drawbacks of multicast transmission, and decrease the power consumption with QoS guarantee. Numerical results based on the conducted theoretical analysis demonstrate that power consumption is reduced with USM while fulfilling the data rate and decoding requirements. In addition, the energy efficiency of USM is superior compared to pure multicast transmission.
Absorption and metabolism of red orange juice anthocyanins in rats

Anthocyanins are natural pigments that could be involved in various health effects. Red oranges are an important dietary source of anthocyanins, including cyanidin 3-glucoside (Cy 3-glc) and an acylated derivative, cyanidin 3-(600-malonyl)-glucoside (Cy 3-malglc). The aim of this study was to evaluate the absorption and metabolism of red orange anthocyanins in rats fed an anthocyanin-enriched diet for 12 d (approximately 2·8 μmol anthocyanins/d). Furthermore, the absorption of these anthocyanins was studied in both the stomach and intestine using in situ models in rats. Anthocyanin metabolites were identified and quantified by HPLC–electrospray ionization tandem MS and HPLC–diode array detection, respectively. The red orange anthocyanins, Cy 3-glc and Cy 3-malglc, as well as their respective methylated derivatives, were recovered in urine after red orange juice intake. The 24 h urinary excretion of total anthocyanins was low (0·081 (SEM 0·009) % of the ingested amount). However, a high proportion (about 20 %) of red orange anthocyanins was absorbed from the stomach. More Cy 3- malglc than Cy 3-glc was absorbed in the intestine. This study thus indicated that red orange juice anthocyanins were rapidly absorbed from both stomach and small intestine, and then excreted in the urine as intact and methylated forms. Moreover, the absorption and metabolism of acylated anthocyanins and non-acylated anthocyanins were similar.
Interactions of Presynaptic Ca2+ Channels and Snare Proteins in Neurotransmitter Release

ABSTRACT: N‐ and P/Q‐type Ca2+ channels are localized in high density in presynaptic nerve terminals and are crucial elements in neuronal excitation‐secretion coupling. In addition to mediating Ca2+ entry to initiate transmitter release, they are thought to interact directly with proteins of the synaptic vesicle docking/fusion machinery. These Ca2+ channels can be purified from brain as a complex with SNARE proteins, which are involved in exocytosis. In addition, N‐type and P/Q‐type Ca2+ channels are colocalized with syntaxin in high‐density clusters in nerve terminals. The synaptic protein interaction (synprint) sites in the intracellular loop II‐III (LII‐III) of both α1B and α1A subunits of N‐type and P/Q‐type Ca2+ channels bind to syntaxin, SNAP‐25, and synaptotagmin.Ca2+ has a biphasic effect on the interactions of N‐type Ca2+ channels with SNARE complexes, stimulating optimal binding in the range of 10–30 μM. PKC or CaM KII phosphorylation of the N‐type synprint peptide inhibits interactions with SNARE complexes containing syntaxin and SNAP‐25. Introduction of the synprint peptides into presynaptic superior cervical ganglion neurons reversibly inhibits EPSPs from synchronous transmitter release by 42%. At physiological Ca2+ concentrations, synprint peptides significantly reduce transmitter release in injected frog neuromuscular junctions in cell culture, consistent with detachment of 70% of the docked vesicles from Ca2+ channels as analyzed by a theoretical model. Together, these studies suggest that presynaptic Ca2+ channels not only provide the Ca2+ signal required by the exocytotic mechinery, but also contain structural elements that are integral to vesicle docking, priming, and fusion processes.
A double‐blind randomized controlled trial of infliximab associated with prednisolone in acute alcoholic hepatitis

Tumor necrosis factor‐α (TNF‐α) may contribute to the progression of acute alcoholic hepatitis (AAH). The aim of this study was to evaluate the efficacy of an association of infliximab and prednisolone at reducing the 2‐month mortality rate among patients with severe AAH. Patients with severe AAH (Maddrey score ≥32) were randomly assigned to group A receiving intravenous infusions of infliximab (10 mg/kg) in weeks 0, 2, and 4; or group B receiving a placebo at the same times. All patients received prednisolone (40 mg/day) for 28 days. Blood neutrophil functional capacities were monitored over 28 days. After randomization of 36 patients, seven patients from group A and three from group B died within 2 months. The probability of being dead at 2 months was higher (not significant [NS]) in group A (39% ± 11%) than in group B (18% ± 9%). The study was stopped by the follow‐up committee and the sponsor (Assistance Publique‐Hôpitaux de Paris). The frequency of severe infections within 2 months was higher in group A than in group B (P < .002). This difference was potentially related to a significantly lower ex vivo stimulation capacity of neutrophils. There were no differences between the two groups in terms of Maddrey scores at any time point. In conclusion, three infusions of 10 mg/kg of infliximab in association with prednisolone may be harmful in patients with severe AAH because of the high prevalence of severe infections. (HEPATOLOGY 2004;39:1390–1397.)
High BCR–ABL/GUSIS Levels at Diagnosis of Chronic Phase CML Are Associated with Unfavorable Responses to Standard-Dose Imatinib

Purpose: The approval of second-generation tyrosine kinase inhibitors (TKIs) for the first-line treatment of chronic myeloid leukemia (CML) has generated an unmet need for baseline molecular parameters associated with inadequate imatinib responses. Experimental Design: We correlated BCR–ABL/GUSIS and BCR–ABL/ABL transcripts at diagnosis with the outcome—defined by the 2013 European LeukemiaNet recommendations—of 272 patients newly diagnosed with CML receiving imatinib 400 mg/daily. Applying receiver-operating characteristic curves, we defined BCR–ABL/GUSIS and BCR–ABL/ABL levels associated with lower probabilities of optimal response, failure-free (FFS), event-free (EFS), transformation-free (TFS), and overall survival (OS). Results: With a median follow-up of 60 months, 65.4% of patients achieved an optimal response (OR), 5.6% were classified as “warnings,” 22.4% failed imatinib, and 6.6% switched to a different TKI because of drug intolerance. We recorded 19 deaths (6.9%), seven (2.5%) attributable to disease progression. We found that higher BCR–ABL/GUSIS levels at diagnosis were associated with inferior rates of OR (P < 0.001), FFS (P < 0.001), and EFS (P < 0.001). Elevated BCR–ABL/GUSIS levels were also associated with lower rates of TFS (P = 0.029) but not with OS (P = 0.132). Similarly, high BCR–ABL/ABL levels at diagnosis were associated with inferior rates of OR (P = 0.03), FFS (P = 0.001), and EFS (P = 0.005), but not with TFS (P = 0.167) or OS (P = 0.052). However, in internal validation experiments, GUS outperformed ABL in samples collected at diagnosis as the latter produced 80% misclassification rates. Conclusions: Our data suggest that high BCR–ABL transcripts at diagnosis measured using GUS as a reference gene identify patients with CML unlikely to benefit from standard-dose imatinib. Clin Cancer Res; 23(23); 7189–98. ©2017 AACR.
Therapy for unresectable hepatocellular carcinoma: review of the randomized clinical trials—II: systemic and local non-embolization-based therapies in unresectable and advanced hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is not only common, but often presents at a stage when potentially curative therapies are not feasible. Although hepatic artery chemoembolization likely confers survival benefit in unresectable HCC, the associated toxicities are substantial and warrant investigation of more efficacious and safe therapies. Many patients who present with unresectable HCC are not chemoembolization candidates, either because of extensive disease or severely impaired hepatic function. We reviewed 44 randomized trials investigating non-embolization-based therapies in unresectable HCC. Hepatic artery infusion of [131I]lipiodol appears safe; initial studies suggest a survival benefit and efficacy comparable to more toxic embolization-based therapies. Some cytotoxic chemotherapy may confer a modest survival benefit in advanced HCC (including oral fluoropyrimidines, and hepatic arterial or i.v. cisplatin and doxorubicin). Tamoxifen does not confer survival benefit, either in advanced or limited HCC. Other therapies warranting further study include interferon (in optimally cytoreduced HCC), megestrol in patients with variant estrogen receptors, octreotide and pravastatin. More adequately powered, rigorously conducted studies will hopefully identify useful chemo-, radio-, immuno-, embolization-based and biologically targeted therapies during the next decade.
Effects of ethanol and ecstasy on conditioned place preference in the rat

The club drug ecstasy (3,4-methylenedioxymethylamphetamine or MDMA) is often taken recreationally with ethanol (EtOH). We have shown previously that EtOH potentiates the psychomotor effects of MDMA in rats. More recently, we demonstrated in striatal slices that MDMA produced preferential release of serotonin, but when combined with EtOH, the preferential release shifted to dopamine, raising the possibility that administration of EtOH may increase the reward effect of MDMA. To address this possibility, adult male Long-Evans rats were tested for conditioned place preference following treatment with saline, EtOH (0.75 g/kg), MDMA (6.6 mg/kg) or the combination. The only condition that produced a preference for the compartment associated with the drug was that of the drug combination. The current data are in line with anecdotal reports and one study in humans, indicating that EtOH alters the pharmacological effects of MDMA including self reports of enhanced or prolonged euphoria. Thus, administration of EtOH might increase the risk for compulsive use of MDMA, an issue that warrants further study.
Evidence that highly conserved residues of transmembrane segment 6 of Escherichia coli MntH are important for transport activity.

Nramp (natural resistance-associated macrophage protein) family members have been characterized in mammals, yeast, and bacteria as divalent metal ion/H(+) symporters. In previous work, a bioinformatic approach was used for the identification of residues that are conserved within the Nramp family [Haemig, H. A. , and Brooker, R. J. (2004) J. Membr. Biol. 201 (2), 97-107]. On the basis of site-directed mutagenesis of highly conserved negatively charged residues, a model was proposed for the metal binding site of the Escherichia coli homologue, MntH. In this study, we have focused on the highly conserved residues, including two histidines, of transmembrane segment 6 (TMS-6). Multiple mutants were made at the eight conserved sites (i.e., Gly-205, Ala-206, Met-209, Pro-210, His-211, Leu-215, His-216, and Ser-217) in TMS-6 of E. coli MntH. Double mutants involving His-211 and His-216 were also created. The results indicate the side chain volume of these residues is critically important for function. In most cases, only substitutions that are closest in side chain volume still permit transport. In addition, the K(m) for metal binding is largely unaffected by mutations in TMS-6, whereas V(max) values were decreased in all mutants characterized kinetically. Thus, these residues do not appear to play a role in metal binding. Instead, they may comprise an important face on TMS-6 that is critical for protein conformational changes during transport. Also, in contrast to other studies, our data do not strongly indicate that the conserved histidine residues play a role in the pH regulation of metal transport.
Long-Term Effect of Social Interactions on Behavioral Plasticity and Lifetime Mating Success

Behavioral traits often change over an individual’s lifetime. Experience, physiological senescence, and age-dependent differences in optimal behavior can, in theory, all cause longitudinal behavioral changes. Yet most studies of behavioral plasticity and selection on behavior focus on short-term population-level responses to social factors such as conspecific density or sex ratio. Longer-term effects of social interactions on individual behavior have rarely been tested. Here we tested these effects by exposing male water striders (Hemiptera: Gerridae) to two different social conditions throughout their lifetime; we call these the nonsocial and social treatments. We then measured each male’s lifetime mating success and individual behavioral plasticity by observing four different behaviors (exploring a novel environment, dispersal ability, sex-recognition sensitivity, and tendency to remount a resistant female after being dislodged) every 2 weeks. The social environment influenced individual variation in behavioral plasticity as well as population-level behavioral plasticity. Moreover, when we calculated linear selection gradients of individual behavioral traits and their plasticities on lifetime mating success, male remounting tendency and individual plasticity in exploration ability were likely to be the most important factors explaining variation in male lifetime mating success. In conclusion, the variation in social interactions throughout an individual’s lifetime contributes to the individual variation in behavioral plasticity, which can significantly affect a male’s lifetime fitness.
Bridging community intervention and mental health services research.

OBJECTIVE This article explores the potential of community intervention perspectives for increasing the relevance, reach, and public health impact of mental health services research. METHOD The authors reviewed community intervention strategies, including public health and community development and empowerment interventions, and contrast community intervention with practice-based quality improvement and policy research. RESULTS A model was proposed to integrate health services and community intervention research, building on the evidence-based strength of quality improvement and participatory methods of community intervention to produce complementary functions, such as linking community-based case finding and referral with practice-based quality improvement, enhanced by community-based social support for treatment adherence. CONCLUSIONS The community intervention approach is a major paradigm for affecting public health or addressing health disparities. Despite challenges in implementation and evaluation, it represents a promising approach for extending the reach of mental health services interventions into diverse communities.
Antidepressant drugs and teenage suicide in Hungary: Time trend and seasonality analysis

Abstract Objective. The aim of the present study was to analyze the relationship between increasing utilization of antidepressants and lithium, and suicide rate of persons less than 20 years of age in Hungary, with particular regard to seasonal patterns. Methods. Time trend analysis was carried out to determine the correlation between antidepressant and lithium prescription patterns in Hungarian persons under age of 20 years as well as seasonal variations within the study period from January 1998 to December 2006. Results. There was a significant correlation (P = 0.03) between the eight-fold increase in antidepressant + lithium prescriptions and decreasing suicides in young Hungarian people under 20 years of age within the study period. Lithium, selective serotonin reuptake inhibitors (SSRIs) and the group of “other antidepressant drugs” rather than nonselective monoamine reuptake inhibitors and monoamine oxidase-A inhibitors were responsible for this association. No significant association could be drawn from seasonal variation with boys (P = 0.964), girls (P = 0.140), or both genders (P = 0.997). Limitation. Ecological study design. Conclusion. Our findings are in good agreement with large-scale ecological studies showing that the beneficial effect of more widely used antidepressants at a given point could appear on the level of suicide rate of the general population even among patients under the age of 20 years.
Development of a Sensitive Electrochemical Enzymatic Reaction-Based Cholesterol Biosensor Using Nano-Sized Carbon Interdigitated Electrodes Decorated with Gold Nanoparticles

We developed a versatile and highly sensitive biosensor platform. The platform is based on electrochemical-enzymatic redox cycling induced by selective enzyme immobilization on nano-sized carbon interdigitated electrodes (IDEs) decorated with gold nanoparticles (AuNPs). Without resorting to sophisticated nanofabrication technologies, we used batch wafer-level carbon microelectromechanical systems (C-MEMS) processes to fabricate 3D carbon IDEs reproducibly, simply, and cost effectively. In addition, AuNPs were selectively electrodeposited on specific carbon nanoelectrodes; the high surface-to-volume ratio and fast electron transfer ability of AuNPs enhanced the electrochemical signal across these carbon IDEs. Gold nanoparticle characteristics such as size and morphology were reproducibly controlled by modulating the step-potential and time period in the electrodeposition processes. To detect cholesterol selectively using AuNP/carbon IDEs, cholesterol oxidase (ChOx) was selectively immobilized via the electrochemical reduction of the diazonium cation. The sensitivity of the AuNP/carbon IDE-based biosensor was ensured by efficient amplification of the redox mediators, ferricyanide and ferrocyanide, between selectively immobilized enzyme sites and both of the combs of AuNP/carbon IDEs. The presented AuNP/carbon IDE-based cholesterol biosensor exhibited a wide sensing range (0.005–10 mM) and high sensitivity (~993.91 µA mM−1 cm−2; limit of detection (LOD) ~1.28 µM). In addition, the proposed cholesterol biosensor was found to be highly selective for the cholesterol detection.
Automatic conversion of Pop music into chiptunes for 8-bit pixel art

In this paper, we propose an audio mosaicing method that converts Pop songs into a specific music style called “chiptune,” or “8-bit music. ” The goal is to reproduce Pop songs by using the sound of the chips on the old game consoles in 1980s/1990s. The proposed method goes through a procedure that first analyzes the pitches of an incoming Pop song in the frequency domain, and then synthesizes the song with template waveforms in the time domain to make it sound like 8-bit music. Because a Pop song is usually composed of the vocal melody and the instrumental accompaniment, in the analysis stage we use a singing voice separation algorithm to separate the vocals from the instruments, and then apply different pitch detection algorithms to transcribe the two separated sources. We validate through a subjective listening test that the proposed method creates much better 8-bit music than existing nonnegative matrix factorization based methods can do. Moreover, we find that synthesis in the time domain is important for this task.
Microarray‐based resequencing by apyrase‐mediated allele‐specific extension

We have developed an array‐based resequencing method to determine genetic alterations in putative cancer genes. The method relies on that the specificity of DNA polymerase in allele‐specific extensions can be enhanced by terminating the extension reactions with apyrase and that a tiling set of primers are synthesized covering the investigated gene sequence. We report on such apyrase‐mediated allele‐specific primer extension (AMASE) assay as a method suitable for high‐throughout resequencing and mutation detection in tumor suppressor genes and oncogenes. In the experimental setup, primers complementary to codons 12, 13 and codon 61 of the N‐ras proto‐oncogene were spotted onto glass slides. Overlapping sense and anti‐sense primers were designed so that complementary primers for all possible mutations in each base position were investigated. The extension reactions were performed in a single step following hybridization of target DNA to the immobilized primers on the array surface. Mutation detection limits and the possibility of quantifying the mutations were investigated using synthetic oligonucleotides. In addition, 64 clinical samples were sequenced and 16 of these showed mutations in the N‐ras gene.
Hierarchical, model‐based merging of multiple fragments for improved three‐dimensional segmentation of nuclei

Automated segmentation of fluorescently labeled cell nuclei in three‐dimensional confocal images is essential for numerous studies, e.g., spatiotemporal fluorescence in situ hybridization quantification of immediate early gene transcription. High accuracy and automation levels are required in high‐throughput and large‐scale studies. Common sources of segmentation error include tight clustering and fragmentation of nuclei. Previous region‐based methods are limited because they perform merging of two nuclear fragments at a time. To achieve higher accuracy without sacrificing scale, more sophisticated yet computationally efficient algorithms are needed.