Miracle-dz/UniFAR-FTUs huggingface dataset:
Thyrotropin regulates tyrosine sulfation of thyroglobulin.

OBJECTIVE To study the regulation of thyroglobulin sulfation by thyrotropin (TSH) and iodide. Sulfation, a widespread post-translational modification of proteins, is involved in various biological activities. Thyroglobulin has been reported to be sulfated but, to date, the role of sulfate residues in the metabolism and function of thyroglobulin is not known; moreover, the regulation of thyroglobulin sulfation has not been yet investigated. METHODS The effect of TSH on thyroglobulin sulfation was studied in porcine thyroid cells cultured on porous collagen-coated filters. Cells cultured with or without TSH and with or without iodide (KI) were incubated for 4 days with radioactive sulfate. The specific radioactivity of thyroglobulin subunit (330kDa) was determined from apical media analyzed by electrophoresis. Enzymatic hydrolysates of the purified thyroglobulin were separated by oligosaccharide affinity chromatography and thin-layer chromatography; alkaline hydrolysates were analyzed only by thin-layer chromatography. RESULTS Thyroglobulin secreted by TSH-stimulated cells incorporated about twofold less radioactive sulfate. Iodide slightly modified this incorporation. Enzymatic hydrolysates of purified thyroglobulin showed sulfate residues bound essentially to complex oligosaccharide units. Alkaline hydrolysis was necessary to release all sulfated amino acids (tyrosine and serine). In the absence of TSH the proportion of tyrosine sulfate was dramatically increased: 24% compared with 7% (+KI) or 5% (-KI). The ratio of specific radioactivity of thyroglobulin to the specific radioactivity of intracellular inorganic sulfate (determined in each culture condition) gave the number of sulfated residues incorporated: 46 (-TSH) and 31 (+TSH) per mol thyroglobulin. From this distribution, we deduced the number of residues bound to complex oligosaccharide units and to tyrosine. Thus TSH decreased the number of sulfate residues on tyrosine from 11 to 2 per mol thyroglobulin. CONCLUSIONS TSH regulates the binding of sulfate groups to tyrosine residues. Iodide exerts a slight control over this process.
Bypass Extended Stack Processing for Anti-Thrashing Replacement in Shared Last Level Cache of Chip Multiprocessors

Chip Multiprocessors (CMPs) allow different applications to share LLC (Last Level Cache). Since each application has different cache capacity demand, LLC capacity should be partitioned in accordance with the demands. Existing partitioning algorithms estimate the capacity demand of each core by stack processing considering the LRU (Least Recently Used) replacement policy only. However, anti-thrashing replacement algorithms like BIP (Binary Insertion Policy) and BIP-Bypass emerged to overcome the thrashing problem of LRU replacement policy in a working set greater than the available cache size. Since existing stack processing cannot estimate the capacity demand with anti-thrashing replacement policy, partitioning algorithms also cannot partition cache space with anti-thrashing replacement policy. In this letter, we prove that BIP replacement policy is not feasible to stack processing but BIP-bypass is. We modify stack processing to accommodate BIP-Bypass. In addition, we propose the pipelined hardware of modified stack processing. With this hardware, we can get the success function of the various capacities with anti-thrashing replacement policy and assess the cache capacity of shared cache adequate to each core in real time. key words: last level cache, stack processing, replacement policy, antithrashing, cache partitioning, chip multi-processors
A CMOS low-distortion switched capacitor oscillator with instantaneous start-up

Experimental results on the oscillator indicate that good accuracy, low distortion, and instantaneous start-up are achieved. The sinusoidal oscillator has been fabricated in a 3-/spl mu/m CMOS double-poly process. The circuit is ideally suited for audio frequency applications such as DTMF tone generators and FSK modems. Further improvements in accuracy are expected if nonideal effects such as infinite op-amp gain and bandwidth and switch imperfections are taken into account.
Molecular Characterization of Laccase Genes from the Basidiomycete Coprinus cinereus and Heterologous Expression of the Laccase Lcc1

ABSTRACT A laccase from Coprinus cinereus is active at alkaline pH, an essential property for some potential applications. We cloned and sequenced three laccase genes (lcc1, lcc2, and lcc3) from the ink cap basidiomycete C. cinereus. The lcc1 gene contained 7 introns, while both lcc2 and lcc3 contained 13 introns. The predicted mature proteins (Lcc1 to Lcc3) are 58 to 80% identical at the amino acid level. The predicted Lcc1 contains a 23-amino-acid C-terminal extension rich in arginine and lysine, suggesting that C-terminal processing may occur during its biosynthesis. We expressed the Lcc1 protein in Aspergillus oryzae and purified it. The Lcc1 protein as expressed in A. oryzae has an apparent molecular mass of 66 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and absorption maxima at 278 and 614 nm. Based on the N-terminal protein sequence of the laccase, a 4-residue propeptide was processed during the maturation of the enzyme. The dioxygen specificity of the laccase showed an apparent Km of 21 ± 2 μM and a catalytic constant of 200 ± 10 min−1for O2 with 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) as the reducing substrate at pH 5.5. Lcc1 from A. oryzae may be useful in industrial applications. This is the first report of a basidiomycete laccase whose biosynthesis involves both N-terminal and C-terminal processing.
Availability of the serotonin transporter in patients with alcohol dependence

Abstract Objectives. Evidence has suggested that the serotonin transporter (SERT) plays a role in the pathogenesis of alcohol dependence, anxiety and depression and that polymorphisms of the serotonin-transporter-linked promoter region (5-HTTLPR) may influence the SERT. This study evaluated the differences in SERT availability between healthy controls and alcoholic patients and the impact of 5-HTTLPR polymorphisms on SERT availability. Methods. Eleven healthy controls and 28 alcoholic patients were recruited. SERT availability was measured in vivo with single photon emission computed tomography and 123I-labelled 2-((2-((dimethyl-amino)methyl)phenyl)thio)-5-iodophenylamine in the midbrain, thalamus and striatum. Each subject was genotyped for the 5-HTTLPR polymorphism. Results. Compared to healthy controls, there was a significantly lower availability of SERT in the midbrain among patients with pure alcohol dependence (pure ALC). Of patients with anxiety, depression and alcohol dependence (ANX/DEPALC), the carriers of one LA allele showed a significantly higher availability of SERT in the striatum compared to non-LA carriers. After Bonferroni correction, these significances vanished. There were no significant differences in SERT availability between controls and ANX/DEP ALC. Conclusions. The results suggest that pure alcoholics may have lower SERT availability in the midbrain; the 5HTTLPR polymorphism may influence SERT availability in ANX/DEP ALC. These findings may serve as a springboard for future large-scale studies.
Promoting innovation in aid of industrial development: the Singaporean experience

Purpose – The purpose of this article is to examine, appraise and highlight the significance of promoting innovation in aid of industrial development.Design/methodology/approach – A comprehensive range of published literature is critiqued first to understand why industrial development constitutes a major objective of a country's economic strategy and government policy. Then, through a theoretical review of industrial policy works, relevant issues (e.g. how efficient industrial development sustains economic growth) are highlighted for discussion. For instance, the importance of private sector‐led industrial development and the need to adopt a primary focus on innovation‐driven industrial policy.Findings – By drawing lessons from the developed world, the article explains why industrial policy‐making must address the pursuit of innovation as a prime mover of economic development; and further outlines the role of government in innovation‐driven industrial policy. As a case study, evolutionary perspectives of .
The Impact of Human and Social Capital on Entrepreneurs’ Knowledge of Finance Alternatives

Building upon prior research that demonstrates how the limited knowledge of finance alternatives of entrepreneurs may cause suboptimal finance decisions, this paper examines how entrepreneurs’ human and social capital influence their knowledge of finance alternatives. For this purpose, we use survey data from 103 Belgian start‐ups. Results demonstrate that entrepreneurs with a business education and entrepreneurs with experience in accountancy or finance have a broader knowledge of finance alternatives. Having a strong network in the financial community is further positively associated with the knowledge of finance alternatives. However, generic human capital, including higher education, industry experience, and management experience, is almost not related with the knowledge of finance alternatives.
Genetic differentiation of Ompok bimaculatus (Teleostei: Siluridae) population based on mtDNA cytochrome b gene

Ompok bimaculatus commonly called as Indian butter catfish is a commercially important food fish and distributed throughout India and Southeast Asia. Genetic differentiation of O. bimaculatus was investigated using cytochrome b (cyt b) gene of three different riverine populations. A total of 37 sequences of cyt b gene (1118 bp) were generated from O. bimaculatus. The mitochondrial DNA analysis revealed that O. bimaculatus has relatively high genetic diversity. The estimated haplotype and nucleotide diversity were 0.063 and 0.005, respectively. The analysis of molecular variance indicated 73.53% genetic variability between the populations and 26.47% genetic variation within population. The understanding of genetic differentiation of O. bimaculatus populations will play a key role in conservation and management of the important endangered fish species.
MULTIPLE EVENT INCIDENCE AND DURATION ANALYSIS FOR CREDIT DATA INCORPORATING NON‐STOCHASTIC LOAN MATURITY

Applications of duration analysis in Economics and Finance exclusively employ methods for events of stochastic duration. In application to credit data, previous research incorrectly treats the time to pre-determined maturity events as censored stochastic event times. The medical literature has binary parametric ‘cure rate' models that deal with populations that never experienced the modelled event. We propose and develop a Multinomial parametric incidence and duration model, incorporating such populations. In the class of cure rate models, this is the first fully parametric multinomial model and is the first framework to accommodate an event with pre-determined duration. The methodology is applied to unsecured personal loan credit data provided by one of Australia's largest financial services organizations. This framework is shown to be more flexible and predictive through a simulation and empirical study that reveals: simulation results of estimated parameters with a large reduction in bias; superior forecasting of duration; explanatory variables can act in different directions upon incidence and duration; and, variables exist that are statistically significant in explaining only incidence or duration.
Restructuring Managerial Labour in the USA, the UK and Japan: Challenging the Salience of ‘Varieties of Capitalism’

This article provides a cross-national, qualitative investigation into the experiences of middle managers in large organizations in the USA, the UK and Japan, following organizational restructuring. Despite well-documented national differences in administrative heritage, institutional regimes or ‘varieties of capitalism’, our data point towards considerable similitude across the three countries in terms of a general expression of the need for change, and the concrete impacts of organizational reforms on managerial work. Specifically we analyse the changing nature of work roles, career paths, working hours and spans of control of mid-level managers in five large firms in each of the three countries. The data demonstrate that middle managers in all three countries face fundamental changes to key areas of their work experience. In Japan, although changes do not amount to a genuine shift towards ‘Anglo-Saxon’ institutions or business practices, the robust use of organizational reforms with very similar aims and underpinning assumptions to those used in the USA and the UK entails similar impacts in terms of work processes of middle managers across the three nations. This shared experience involved the augmentation of middle management skill levels, responsibilities and span of control, but alongside the downgrading of career expectations, and increased workload and work intensity. We argue that these changes are in keeping with some, but not all, of the features explained and predicted in Bravermanian labour process theory.
HSF-1 Interacts with Ral-binding Protein 1 in a Stress-responsive, Multiprotein Complex with HSP90 in Vivo *

Heat shock factor 1 (HSF1) regulates the rapid and transient expression of heat shock genes in response to stress. The transcriptional activity of HSF1 is tightly controlled, and under physiological growth conditions, the HSF1 monomer is in a heterocomplex with the molecular chaperone HSP90. Through unknown mechanisms, transcriptionally repressed HSF1·HSP90 heterocomplexes dissociate following stress, which triggers HSF1 activation and heat shock gene transcription. Using a yeast two-hybrid screening system, we have identified Ral-binding protein 1 (RalBP1) as an additional HSF1-interacting protein. We show that RalBP1 and HSF1 interact in vivo, and transient cotransfection of HSF1 and RalBP1 into hsf1 −/− mouse embryo fibroblasts represses HSP70 expression. Furthermore, transient cotransfection of HSF1 and the constitutively active form of RalA (RalA23V), an upstream activator of the RalBP1 signaling pathway, increases the heat-inducible expression of HSP70, whereas the dominant negative form (RalA28N) suppresses HSP70 expression. We further find that α-tubulin and HSP90 are also present in the RalBP1·HSF1 heterocomplexes in unstressed cells. Upon heat shock, the Ral signaling pathway is activated, and the resulting RalGTP binds RalBP1. Concurrently, HSF1 is activated, leaves the RalBP1·HSF1·HSP90·α-tubulin heterocomplexes, and translocates into the nucleus, where it then activates transcription. In conclusion, these observations reveal that the RalGTP signal transduction pathway is critical for activation of the stress-responsive HSF1 and perhaps HSP90 molecular chaperone system.
Modelling High-Intensity Crime Areas: Comparing Police Perceptions with Offence/Offender Data in Sheffield

High-intensity crime areas are areas where high levels of violent crime coexist with large numbers of offenders, thereby creating an area that may present significant policing problems. In an earlier paper, the authors analysed police perceptions of high-intensity crime areas, and now extend that earlier work by comparing the police's perception of where such areas are located with offence/offender data. They also report on the construction of predictive models that identify the area-specific attributes that explain the distribution of such areas. By focusing on the city of Sheffield, the authors draw on a wider range of local area data than was possible in the original paper, and also question how widespread such areas may be in Sheffield.
Schizophrenia two‐hit hypothesis in velo‐cardio facial syndrome

Deletion of chr22q11 gives rise to velo‐cardio facial syndrome (VCFS) and increases schizophrenia risk. The source of this elevated risk although unknown could result from stochastic, environmental, or genetic factors, the latter encompassing a range of complexity from polygenic mechanisms to “second‐hit” mutations. For this study we tested the two‐hit hypothesis where additional risk is conferred through a second CNV. We identified large (>100 kb) CNVs in 48 VCFS cases (23 with psychosis—25 without) and show in the psychotic VCFS group there is a significant (P = 0.02) increase in the average size of CNVs (354–227 kb). To identify second‐hit loci we focused on individuals possessing gene‐centric CNVs and through literature mining identified 4 (31%) psychotic VCFS individuals (n = 13) that overlapped loci previously implicated in neuropsychiatric disorders compared to 1 (10%) from the non‐psychotic VCFS individuals (n = 10). For replication 17 VCFS patients with schizophrenia from the molecular genetics of schizophrenia dataset were used to identify further CNVs. Thirteen individuals possessing gene‐centric CNVs were identified including 3 (23%) individuals possessing a potential second‐hit, taking the overall total in the psychotic VCFS group (n = 26) to 7 (27%) potential second‐hit loci. Notably a deletion in a psychotic VCFS patient at 2q23.1 hit the gene MBD5 which when deleted gives rise to intellectual disability, epilepsy, and autistic features. Through this study we potentially extend this phenotypic spectrum to include schizophrenia. Our results suggest the two‐hit hypothesis may be relevant to a proportion of VCFS patients with psychosis but sample sizes are small and further studies warranted. © 2013 Wiley Periodicals, Inc.
Corporate Incentives to Disclose Carbon Information: Evidence from the CDP Global 500 Report

We investigate how the Global 500 companies respond to the challenge of climate change with regard to their carbon disclosure strategies. This paper is motivated by a growing body of research that examines the role of large companies in carbon disclosure responsibility and practices. We consider the impact of social, financial market, economic, regulatory, and institutional factors on the motivation to voluntarily participate in the 2009 Carbon Disclosure Project. We find that economic pressure is significantly associated with the decision. That is, companies facing direct economic consequence are more likely to disclose. Companies in greenhouse gas (GHG) intensive sectors show the same tendency. In addition, big companies have a higher propensity for disclosing, suggesting that social pressure plays an important role. We also provide possible explanations as to why a large proportion of our sample firms refuse to disclose. Furthermore, our results suggest that the proxies for information needs of investors are not associated with a higher propensity to disclose the amount of their emission footprints. In sum, it appears that the major driving force for climate change disclosure comes from the general public and government rather than from the other major stakeholders such as shareholders and debtholders. Our results are robust after controlling for other influences.
Detecting Spot Price Forecasts in Futures Prices

Futures markets are often described as having two important social functions. First, they facilitate the transfer of commodity price risk, and, second, they provide forecasts of commodity prices. The evidence that futures markets transfer price risk is irrefutable. However, there is some debate about the markets' forecasting ability. In particular, forecasts based on the current spot price are often as good as those based on the futures price. Some economists cite a failure to detect superior forecast power in futures prices as evidence of market inefficiency (see, e.g., Leuthold 1974; and Martin and Garcia 1981). There are at least two other explanations. First, there may be nothing for the futures market to forecast. If the current spot price equals the true expectation of the future spot price, the futures market cannot provide a better forecast. Second, a superior futures market forecast may be obscured by the unexpected component of the realized spot price. The true expectation of the future spot price is unobservable; one must approximate this expectation with the actual future spot price.
The Laws of Human Relations and the Rules of Human Action Derived Therefrom

In the history of the exact sciences there have been a number of momentous contributions that were not recognized until years after they were made: Carnot's insights in thermodynamics, Galois's in algebra, Mendel's in genetics and, in the social science of political economy, Hermann Gossen's "Laws of Human Relations," which laid the foundation of modern utility theory.This is the first English translation of a book initially published in 1854 that provides historians with the link necessary for accurately describing the development of economics as a mathematical and analytic discipline. Gossen's important and original work was ignored in its own time and, until now, surfaced periodically only in the context of other studies.Hermann Gossen (1810-1859) never held an academic position and resigned from a lackluster career in the Prussian Civil Service in order to complete work on his book. "I believe," he wrote of it, "that my discoveries enable me to point out to man with unfailing certainty the path that he must follow in order to accomplish completely the purpose of his life. "The path that he sketched for his readers - which was to be laid down in accordance with the principle of diminishing marginal utility and the corresponding theorem of the maximization of utility - preceded William Stanley Jevon's, Carl Menger's and Leon Walras's versions of what was in the mid- to late 1800s a new theory of value and the basis of modern economics. Why his work remained in obscurity is a puzzle teased out in Georgescu-Roegen's long introductory essay on Gossen's life and the significance of this book.Publication of "The Laws of Human Relations" is supported by a grant from the National Endowment for the Humanities.
The Role of Allopurinol and Deferoxamine in Preventing Pressure Ulcers in Pigs

Ischemia and reperfusion may be important in the pathogenesis of pressure ulcers. On the basis of this hypothesis, the effects of intermittent pressure and the antifree radical agents allopurinol and deferoxamine were studied in a pig model in which a pressure of 150 mmHg was applied intermittently to the scapulae. Cutaneous blood flow, transcutaneous oxygen tension, skin and muscle damage, and muscle levels of adenosine triphosphate were quantified. A control group of pigs (n = 6) was untreated, the allopurinol group (n = 6) received oral allopurinol beginning 2 days before the experiment, and the deferoxamine group (n = 6) received an intramuscular injection of deferoxamine 2 hours before the experiment. Pressure (150 mmHg) was applied to the scapulae for 210 minutes, and it was relieved for 30 minutes. This 4‐hour cycle was repeated continuously for 48 hours, and it resulted in pressure injuries in all animals. Allopurinol and deferoxamine improved cutaneous blood flow and tissue oxygenation, but only deferoxamine could significantly reduce cutaneous and skeletal muscle necrosis (p < 0.001). This study suggests a future role for antifree radical agents in the reduction of pressure‐induced injury. (Plast. Reconstr. Surg. 105: 1408, 2000.)