Miracle-dz/UniFAR-FTUs huggingface dataset:
Toward Automating Clinical Assessments: A Survey of the Timed Up and Go

Older adults often suffer from functional impairments that affect their ability to perform everyday tasks. To detect the onset and changes in abilities, healthcare professionals administer standardized assessments. Recently, technology has been utilized to complement these clinical assessments to gain a more objective and detailed view of functionality. In the clinic and at home, technology is able to provide more information about patient performance and reduce subjectivity in outcome measures. The timed up and go (TUG) test is one such assessment recently instrumented with technology in several studies, yielding promising results toward the future of automating clinical assessments. Potential benefits of technological TUG implementations include additional performance parameters, generated reports, and the ability to be self-administered in the home. In this paper, we provide an overview of the TUG test and technologies utilized for TUG instrumentation. We then critically review the technological advancements and follow up with an evaluation of the benefits and limitations of each approach. Finally, we analyze the gaps in the implementations and discuss challenges for future research toward automated self-administered assessment in the home.
Low-Dose, Long-Wave UV Light Does Not Affect Gene Expression of Human Mesenchymal Stem Cells

Light is a non-invasive tool that is widely used in a range of biomedical applications. Techniques such as photopolymerization, photodegradation, and photouncaging can be used to alter the chemical and physical properties of biomaterials in the presence of live cells. Long-wave UV light (315 nm–400 nm) is an easily accessible and commonly used energy source for triggering biomaterial changes. Although exposure to low doses of long-wave UV light is generally accepted as biocompatible, most studies employing this wavelength only establish cell viability, ignoring other possible (non-toxic) effects. Since light exposure of wavelengths longer than 315 nm may potentially induce changes in cell behavior, we examined changes in gene expression of human mesenchymal stem cells exposed to light under both 2D and 3D culture conditions, including two different hydrogel fabrication techniques, decoupling UV exposure and radical generation. While exposure to long-wave UV light did not induce significant changes in gene expression regardless of culture conditions, significant changes were observed due to scaffold fabrication chemistry and between cells plated in 2D versus encapsulated in 3D scaffolds. In order to facilitate others in searching for more specific changes between the many conditions, the full data set is available on Gene Expression Omnibus for querying.
Increased intra-abdominal pressure: is it of any consequence in severe acute pancreatitis?

BACKGROUND Increased intra-abdominal pressure (IAP) is detrimental for the recovery of organ function in trauma and emergency patients. The aim of this study was to assess the correlation between the dynamics of IAP and organ dysfunction in severe acute pancreatitis (SAP). MATERIALS AND METHODS Management of SAP between 2000 and 2004 was analysed. SAP was classified according to Atlanta 1992. Organ dysfunction, systemic inflammatory response syndrome (SIRS) and outcomes in relation to the IAP were assessed. IAP was measured indirectly. RESULTS A total of 65 patients, with an average APACHE II score of 6.44, complied with the Atlanta criteria. In all, 34 patients received conservative treatment and 31 were operated. SIRS was observed in 59 cases and multiple organ dysfunction syndrome (MODS) in 61 cases. IAP was significantly higher in the 25 most complicated patients requiring renal replacement therapy (RRT), compared with 40 patients without RRT, 31.72 vs 21.4 cm/H(2)O (p=0.037). IAP interrelated positively with SOFA score (r = + 0.371, p<0.01) and organs involved (r = + 0.356, p<0.01), and negatively with platelet count and enterally provided volume (r = - 0.284, p<0.01; r = - 0.5, p<0.01, respectively). Overall mortality (9.2%) was associated with surgery and sustained increase of the IAP over 25 cm/H(2)O. Our data support the pathophysiological interrelation of elevated IAP and development of organ dysfunction. CONCLUSION Development of organ dysfunction in SAP could be associated with increased IAP. Grade III increase of IAP should be considered as an indicator for revision of treatment modalities.
Impact of Prescribed Exercise on Physical Activity Compensation in Young Adults

Abstract Liguori, G, Schuna, JM Jr, Tucker, J, and Fountaine, CM. Impact of prescribed exercise on physical activity compensation in young adults. J Strength Cond Res 31(2): 503–508, 2017—Army Reserve Officers' Training Corps cadets present a unique subpopulation because they are required to participate in regular physical activity (PA). This study describes PA patterns of cadets and attempts to identify evidence of nonexercise PA compensation (activitystat) as a result of prescribed PA (pPA) by comparing differences between training and nontraining days for (a) autonomous PA among cadets and (b) PA between cadets and noncadets. Participants included 84 university students (33 cadets and 51 noncadets) who each wore an accelerometer for 5 consecutive days to estimate moderate and vigorous physical activity (MVPA). A 2×2 mixed model analysis of variance was used to examine within- and between-group differences in MVPA on training and nontraining days. This analysis was repeated after removing the cadet's pPA. Cadets had lower-body fat than noncadets (p = 0.044), but all other characteristics were similar. Overall, moderate PA (MPA) and vigorous PA (VPA) were significantly greater among cadets (p = 0.048 and p < 0.001), because of greater weekend MPA (p = 0.021) and greater weekday VPA (p < 0.001). Cadets accumulated more MVPA on training days than nontraining days (p < 0.001) and accumulated more MVPA than noncadets on training days (p = 0.004). However, after accounting for pPA, cadet MVPA did not differ between training and nontraining days (within 1.2Â ± 18.4 min·d−1) and was similar between cadets and noncadets (within 1.5Â ± 5.9 min·d−1). These results suggest that cadets were significantly more active than noncadets due mainly to pPA. When controlling for pPA, cadets were similarly active across all days, and were as active as noncadets, indicating no evidence of activitystat in this population.
Transformation of hematopoietic cells by BCR/ABL requires activation of a PI‐3k/Akt‐dependent pathway

The BCR/ABL oncogenic tyrosine kinase activates phosphatidylinositol 3‐kinase (PI‐3k) by a mechanism that requires binding of BCR/ABL to p85, the regulatory subunit of PI‐3k, and an intact BCR/ABL SH2 domain. SH2 domain BCR/ABL mutants deficient in PI‐3k activation failed to stimulate Akt kinase, a recently identified PI‐3k downstream effector with oncogenic potential, but did activate p21 RAS and p70 S6 kinase. The PI‐3k/Akt pathway is essential for BCR/ABL leukemogenesis as indicated by experiments demonstrating that wortmannin, a PI‐3k specific inhibitor at low concentrations, suppressed BCR/ABL‐dependent colony formation of murine marrow cells, and that a kinase‐deficient Akt mutant with dominant‐negative activity inhibited BCR/ABL‐dependent transformation of murine bone marrow cells in vitro and suppressed leukemia development in SCID mice. In complementation assays using mouse marrow progenitor cells, the ability of transformation‐defective SH2 domain BCR/ABL mutants to induce growth factor‐independent colony formation and leukemia in SCID mice was markedly enhanced by expression of constitutively active Akt. In retrovirally infected mouse marrow cells, the BCR/ABL mutant lacking the SH2 domain was unable to upregulate the expression of c‐Myc and Bcl‐2; in contrast, expression of a constitutively active Akt mutant induced Bcl‐2 and c‐Myc expression, and stimulated the transcription activation function of c‐Myc. Together, these data demonstrate the requirement for the BCR/ABL SH2 domain in PI‐3k activation and document the essential role of the PI‐3k/Akt pathway in BCR/ABL leukemogenesis.
Why Has U.S. Inflation Become Harder to Forecast

Forecasts of the rate of price inflation play a central role in the formulation of monetary policy, and forecasting inflation is a key job for economists at the Federal Reserve Board. This paper examines whether this job has become harder and, to the extent that it has, what changes in the inflation process have made it so. The main finding is that the univariate inflation process is well described by an unobserved component trend-cycle model with stochastic volatility or, equivalently, an integrated moving average process with time-varying parameters; this model explains a variety of recent univariate inflation forecasting puzzles. It appears currently to be difficult for multivariate forecasts to improve on forecasts made using this time-varying univariate model.
Crosstalk modelling and analysis of interconnects based on carbon nanotubes bundles

In this paper, the crosstalk problem for carbon nanotubes (CNTs) bundle interconnects is dealt with. The CNT bundle is modelled by using the multiconductor transmission line (MTL) theory and, from this, a compact coupled equivalent conductors (CEC) model is derived. The influence of two modelling uncertainties is investigated. In particular, the pattern of the conducting CNTs in the transverse cross section and the size of the terminations. The results obtained for the full MTL model are compared to those obtained by CEC.
Do credit ratings affect spread and return? A study of structured finance products

Although the previous studies investigating the relationship between credit ratings and spread or return in the financial market are normally restricted to noncausal measures, this paper uses structural equation modelling to test the possibility of causal links from ratings to spread and return in the context of structured finance products. Our analyses are split into 2 stages: First, we search for causality between ratings and spread at the issuance stage (primary market) based on a sample comprising all tranches of assetâ€ backed securities issued in the United States by American and foreign institutions from December 1999 to December 2015. Then, we consider all ABS rating changes from February 2001 to December 2015 to check whether the assumption of causal connection between ratings and return at the trading stage (secondary market) is reasonable. After testing all pertinent combinations among the variables in our database, we find evidence of causality at the issuance stage but very little support to causality at the trading stage. This study contributes to the debate on the regulation of credit rating agencies as our findings suggest that ratings may have an effective influence on decisions made by investors at the time structure finance products are issued. As this effect is very weak when those assets are traded in the secondary market, in principle, regulators should focus their attention on the credit rating agencies' activities regarding the issuance of new structured products.
High CMRR and low THD current-mode instrumentation amplifier using current inversion technique

The current-mode instrumentation amplifier based on second-generation current conveyors (CCII) offers many benefits over conventional instrumentation amplifier architectures. It does not need any matched components to achieve high CMRR and its bandwidth is not gain-bandwidth product limited. Therefore, a wideband CMOS current conveyor has been used in this structure. HSPICE simulations have shown that its performance is quite satisfactory for wideband current-mode signal processing. Furthermore, CMRR enhancement technique of current-mode instrumentation amplifiers is discussed. In addition, composite conveyor techniques which improve the instrumentation amplifier performance are presented. The new simulated instrumentation amplifier provides approximately 44 dB of improvement on the high-frequency CMRR up to the 3-dB corner frequency and even higher. Also a THD of %0.6 has been achieved.
Naturally processed viral peptides recognized by cytotoxic T lymphocytes on cells chronically infected by human immunodeficiency virus type 1

We have established long-term cultures of several cell lines stably and uniformly expressing human immunodeficiency virus type 1 (HIV-1) in order to (a) identify naturally processed HIV-1 peptides recognized by cytotoxic T lymphocytes (CTL) from HIV-1-seropositive individuals and (b) consider the hypothesis that naturally occurring epitope densities on HIV-infected cells may limit their lysis by CTL. Each of two A2- restricted CD8+ CTL specific for HIV-1 gag or reverse transcriptase (RT) recognized a single naturally processed HIV-1 peptide in trifluoroacetic acid (TFA) extracts of infected cells: gag 77-85 (SLYNTVATL) or RT 476-484 (ILKEPVHGV). Both processed peptides match the synthetic peptides that are optimally active in cytotoxicity assays and have the consensus motif described for A2-associated peptides. Their abundances were approximately 400 and approximately 12 molecules per infected Jurkat-A2 cell, respectively. Other synthetic HIV-1 peptides active at subnanomolar concentrations were not present in infected cells. Except for the antigen processing mutant line T2, HIV- infected HLA-A2+ cell lines were specifically lysed by both A2- restricted CTL, although infected Jurkat-A2 cells were lysed more poorly by RT-specific CTL than by gag-specific CTL, suggesting that low cell surface density of a natural peptide may limit the effectiveness of some HIV-specific CTL despite their vigorous activity against synthetic peptide-treated target cells.
Sitting Silently at Home: A Critique of The Revolution Will Not Be Funded: Beyong the Non-Profit Industrial Complex

I. THE NON-PROFIT INDUSTRIAL COMPLEX: DEFINITIONS AND QUESTIONS . 279 A. Who Are the Members of the Non-Profit Industrial Complex? What Do They Really D o? 282 B. How Can These Organizations Contribute to the Establishment of a Transformative Social Justice Movement? 289 II. WHAT Is A SOCIAL MOVEMENT? 293 A. How Is "Organizing" Different from a "Social M ovem ent? " . 294 B. If This Isn't a Social Movement, Then What Is It? 298 III. CONCLUSION . 303
A Simple Space Vector PWM Generation Scheme for Any General  $n$-Level Inverter

This paper proposes a generalized method for the generation of space vector pulsewidth modulation (SVPWM) signals for multilevel inverters. In the proposed method, the actual sector containing the tip of the reference space vector need not be identified. A method is presented to identify the center of a sub-hexagon containing the reference space vector. Using the center of the sub-hexagon, the reference space vector is mapped to the innermost sub-hexagon, and the switching sequence corresponding to a two-level inverter is determined. A new technique is proposed in this paper, by which these two-level vectors are translated to the switching vectors of the multilevel inverter by adding the center of the sub-hexagon to the two-level vectors. The proposed method can be extended to any n-level inverter, and a generalized algorithm is proposed. The scheme is explained for a five-level inverter, and experimental results are presented for a three-level inverter.
Prognostic value of p53 genetic changes in colorectal cancer.

PURPOSE To explore whether there is a linkage between different mutations in the p53 gene in primary colorectal cancer and the risk of death from colorectal cancer in a large group of patients with long follow-up. We also compared a complementary DNA-based sequencing method and an immunohistochemical (IHC) method for detecting p53 protein overexpression in colorectal cancer. MATERIALS AND METHODS The entire coding region of the p53 gene was sequenced in 191 frozen tumor samples collected from January 1988 to November 1992. RNA was extracted and synthesized to cDNA. p53 was amplified by the polymerase chain reaction, and the DO-7 monoclonal antibody was used in the IHC assessments. RESULTS Mutations were detected in 99 samples (52%) from 189 patients. There was a significant relationship between the p53 mutational status and the cancer-specific survival time, with shorter survival time for patients who had p53 mutations than for those who did not (P = .01, log-rank test). Mutations outside the evolutionarily conserved regions were associated with the worst prognosis. Multivariate analysis showed that the presence of p53 mutations was an independent prognostic factor (relative hazard, 1.7, P = .03). There was no significant relationship between overexpression of p53 protein, as determined by IHC analysis, and cancer-specific survival. CONCLUSION Mutational analyses of the p53 gene, using cDNA sequencing in colorectal cancer, provide useful prognostic information. In addition, cDNA sequencing gives better prognostic information than IHC assessment of p53 protein overexpression.
National contributions to climate change mitigation from agriculture: allocating a global target

ABSTRACT Globally, agriculture and related land use change contributed about 17% of the world’s anthropogenic GHG emissions in 2010 (8.4 GtCO2e yr−1), making GHG mitigation in the agriculture sector critical to meeting the Paris Agreement’s 2°C goal. This article proposes a range of country-level targets for mitigation of agricultural emissions by allocating a global target according to five approaches to effort-sharing for climate change mitigation: responsibility, capability, equality, responsibility-capability-need and equal cumulative per capita emissions. Allocating mitigation targets according to responsibility for total historical emissions or capability to mitigate assigned large targets for agricultural emission reductions to North America, Europe and China. Targets based on responsibility for historical agricultural emissions resulted in a relatively even distribution of targets among countries and regions. Meanwhile, targets based on equal future agricultural emissions per capita or equal per capita cumulative emissions assigned very large mitigation targets to countries with large agricultural economies, while allowing some densely populated countries to increase agricultural emissions. There is no single ‘correct’ framework for allocating a global mitigation goal. Instead, using these approaches as a set provides a transparent, scientific basis for countries to inform and help assess the significance of their commitments to reducing emissions from the agriculture sector. Key policy insights Meeting the Paris Agreement 2°C goal will require global mitigation of agricultural non-CO2 emissions of approximately 1 GtCO2e yr−1 by 2030. Allocating this 1 GtCO2e yr−1 according to various effort-sharing approaches, it is found that countries will need to mitigate agricultural business-as-usual emissions in 2030 by a median of 10%. Targets vary widely with criteria used for allocation. The targets calculated here are in line with the ambition of the few countries (primarily in Africa) that included mitigation targets for the agriculture sector in their (Intended) Nationally Determined Contributions. For agriculture to contribute to meeting the 2°C or 1.5°C targets, countries will need to be ambitious in pursuing emission reductions. Technology development and transfer will be particularly important.
Characterization of Tn1546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147

Sequence determination of the flanking regions of the vancomycin resistance van gene cluster carried by pIP816 in Enterococcus faecium BM4147 revealed similarity to transposons of the Tn3 family. Imperfect inverted repeats (36 of 38 bp) delineated a 10,851-bp element designated Tn1546. The 4-kb region located upstream from the vanR gene contained two open reading frames (ORF) transcribed in opposite directions. The deduced amino acid sequence of ORF1 (988 residues) displayed, respectively, 56 and 42% identity to those of the transposases of Tn4430 from Bacillus thuringiensis and of Tn917 from Enterococcus faecalis. The product of ORF2 (191 residues) was related to the resolvase of Tn917 (33% amino acid identity) and to the Res protein (48%) of plasmid pIP404 from Clostridium perfringens. Tn1546 transposed consecutively in Escherichia coli from plasmid pUC18 into pOX38 and from pOX38 into various sites of pBR329. Transposition was replicative, led to the formation of cointegrates, and produced a 5-bp duplication at the target site. Southern hybridization and DNA amplification revealed the presence of Tn1546-related elements in enterococci highly resistant to glycopeptides. Analysis of sequences surrounding these elements indicated that transposition plays a role in dissemination of the van gene cluster among replicons of human clinical isolates of E. faecium.
Opposing Functions of the N-terminal Acetyltransferases Naa50 and NatA in Sister-chromatid Cohesion*

During the cell cycle, sister-chromatid cohesion tethers sister chromatids together from S phase to the metaphase-anaphase transition and ensures accurate segregation of chromatids into daughter cells. N-terminal acetylation is one of the most prevalent protein covalent modifications in eukaryotes and is mediated by a family of N-terminal acetyltransferases (NAT). Naa50 (also called San) has previously been shown to play a role in sister-chromatid cohesion in metazoans. The mechanism by which Naa50 contributes to cohesion is not understood however. Here, we show that depletion of Naa50 in HeLa cells weakens the interaction between cohesin and its positive regulator sororin and causes cohesion defects in S phase, consistent with a role of Naa50 in cohesion establishment. Strikingly, co-depletion of NatA, a heterodimeric NAT complex that physically interacts with Naa50, rescues the sister-chromatid cohesion defects and the resulting mitotic arrest caused by Naa50 depletion, indicating that NatA and Naa50 play antagonistic roles in cohesion. Purified recombinant NatA and Naa50 do not affect each other's NAT activity in vitro. Because NatA and Naa50 exhibit distinct substrate specificity, we propose that they modify different effectors and regulate sister-chromatid cohesion in opposing ways.
Energy expenditure climbing Mt. Everest.

Weight loss is a well-known phenomenon at high altitude. It is not clear whether the negative energy balance is due to anorexia only or an increased energy expenditure as well. The objective of this study was to gain insight into this matter by measuring simultaneously energy intake, energy expenditure, and body composition during an expedition to Mt. Everest. Subjects were two women and three men between 31 and 42 yr of age. Two subjects were observed during preparation at high altitude, including a 4-day stay in the Alps (4,260 m), and subsequently during four daytime stays in a hypobaric chamber (5,600-7,000 m). Observations at high altitude on Mt. Everest covered a 7- to 10-day interval just before the summit was reached in three subjects and included the summit (8,872 m) in a fourth. Energy intake (EI) was measured with a dietary record, average daily metabolic rate (ADMR) with doubly labeled water, and resting metabolic rate (RMR) with respiratory gas analysis. Body composition was measured before and after the interval from body mass, skinfold thickness, and total body water. Subjects were in negative energy balance (-5.7 +/- 1.9 MJ/day) in both situations, during the preparation in the Alps and on Mt. Everest. The loss of fat mass over the observation intervals was 1.4 +/- 0.7 kg, on average two-thirds of the weight loss (2.2 +/- 1.5 kg), and was significantly correlated with the energy deficit (r = 0.84, P < 0.05). EI on Mt. Everest was 9-13% lower than during the preparation in the Alps. (ABSTRACT TRUNCATED AT 250 WORDS)