Miracle-dz/UniFAR-FTUs huggingface dataset:
Cyclic AMP-dependent Protein Lysine Acylation in Mycobacteria Regulates Fatty Acid and Propionate Metabolism*

Background: KATmt is the first identified cAMP-regulated protein lysine acetylase in mycobacteria. Results: KATmt acylates fatty acyl CoA ligases in vivo in a cAMP-dependent manner, thus regulating their activity. Conclusion: Mycobacteria utilize KATmt to regulate the metabolic pool of acetyl and propionyl CoA. Significance: We provide novel paradigms for linking cAMP signaling and fatty acid metabolism in mycobacteria. Acetylation of lysine residues is a posttranslational modification that is used by both eukaryotes and prokaryotes to regulate a variety of biological processes. Here we identify multiple substrates for the cAMP-dependent protein lysine acetyltransferase from Mycobacterium tuberculosis (KATmt). We demonstrate that a catalytically important lysine residue in a number of FadD (fatty acyl CoA synthetase) enzymes is acetylated by KATmt in a cAMP-dependent manner and that acetylation inhibits the activity of FadD enzymes. A sirtuin-like enzyme can deacetylate multiple FadDs, thus completing the regulatory cycle. Using a strain deleted for the KATmt ortholog in Mycobacterium bovis Bacillus Calmette-Guérin (BCG), we show for the first time that acetylation is dependent on intracellular cAMP levels. KATmt can utilize propionyl CoA as a substrate and, therefore, plays a critical role in alleviating propionyl CoA toxicity in mycobacteria by inactivating acyl CoA synthetase (ACS). The precision by which mycobacteria can regulate the metabolism of fatty acids in a cAMP-dependent manner appears to be unparalleled in other biological organisms and is ideally suited to adapt to the complex environment that pathogenic mycobacteria experience in the host.
Amplification and increased expression of alpha class glutathione S-transferase-encoding genes associated with resistance to nitrogen mustards.

Glutathione-dependent enzymes play a central role in the protection of cells from cytotoxic chemicals and have been implicated in the intrinsic and acquired resistance of tumors to cytotoxic drugs. We have generated a Chinese hamster ovary line resistant to bifunctional nitrogen mustards and in this report have characterized and isolated the protein that represents the major observable phenotypic difference between the drug-sensitive and drug-resistant cell lines. This purified protein is shown to be an alpha class glutathione S-transferase comprising YcYc subunits and possessing a pI value of approximately 8.0. The intracellular level of the Yc subunit is elevated greater than 40-fold in the drug-resistant cell line, which could account for the increase in glutathione S-transferase (RX:glutathione R-transferase; EC 2.5.1.18) activity toward both 1-chloro-2,4-dinitrobenzene and cumene hydroperoxide. Other glutathione S-transferase subunits within this gene family are also elevated. These changes are accompanied by a significant elevation in alpha class mRNA levels. Southern analysis indicates that the genes coding for these proteins are amplified 4- to 8-fold in the drug-resistant cell line. In addition, gamma-glutamyl transpeptidase [(5-glutamyl)-peptide:amino acid 5-glutamyltransferase; EC 2.3. 2.2] activity is increased 3.6-fold in the drug-resistant Chinese hamster ovary cell line, which may explain the increase in cellular glutathione level. In this case no gene amplification was seen. These data indicate that gene amplification may be important in drug resistance toward alkylating agents and also that other enzymes in glutathione homeostasis are involved.
In vivo release profiles of leuprolide acetate from microcapsules prepared with polylactic acids or copoly(lactic/glycolic) acids and in vivo degradation of these polymers.

To screen a suitable polymer for preparing a controlled-release dosage form effective for one month, square plates of polyactic acid (PLA) with average molecular weights of 6000 to 50000 and copoly(lactic/glycolic) acid (PLGA) with average molecular weights of 6000 to 13500 and a copolymer ratio of 90/10 to 55/45 were implanted in rats subcutaneously, and the weight losses were determined over 100 d. Typically the pattern of weight loss was biphasic with a lag time followed by a period when the weight fell exponentially. The lag times and the half lives of the degradation period increased with increase in the molecular weight or with decrease in glycolide content. Release of leuprolide acetate from PLA microcapsules at the injection site in rats (in vivo release) tended to be comparable to the bioerosion rate of the polymer used as the wall substance, and the in vivo release of the drug from PLGA microcapsules tended to be comparable to the biodegradation rate of the polymer. The in vivo release profile of the drug from microcapsules prepared with PLGA of average molecular weight 14000 and copolymer ratio 75/25 was ideal for one month's controlled release. The in vivo release profile from microcapsules injected subsutaneously in rats was comparable to the in vitro release.
Carte d'énergie pour l'oscillateur élastoplastique en régime dynamique

L'article a pour ambition de traiter du comportement des structures elastoplastiques soumises a des sollicitations periodiques de type sismique. L'etude est restreinte a un systeme a un degre de liberte. La frontiere entre l'adaptation et l'accomodation est donnee en fonction des parametres structuraux du systeme. Une etude numerique montre la convergence des trajectoires vers des orbites periodiques. Enfin, une carte d'energie dissipee dans la zone d'accommodation est etablie en fonction des parametres de sollicitations sismiques.
Molecular analysis of childhood primitive neuroectodermal tumors defines markers associated with poor outcome.

PURPOSE The diagnostic and prognostic significance of well-defined molecular markers was investigated in childhood primitive neuroectodermal tumors (PNET). MATERIALS AND METHODS Using microsatellite analysis, Southern blot analysis, and fluorescence in situ hybridization (FISH), 30 primary tumors and six CSF metastasis specimens were analyzed for loss of heterozygosity (LOH) of chromosomes 1q31, 6q, 9q22, 10q, 11, 16q22, and 17p13.1 and/or high-level amplification of the c-myc gene. Experimental data were compared with clinical stage and outcome. RESULTS LOH of chromosome 17p13.1 was found most frequently (14 of 30 tumors, six of six CSF metastasis specimens); LOH of chromosomes 10q, 16q22, 11, 6, 9q22, and 1q31 was observed in 20.6%, 20%, 14.3%, 12%, 10%, and 0%, respectively. Eight of 32 tumors and CSF specimens showed amplification of c-myc. All tumors with amplification of c-myc were resistant to therapy and had a fatal outcome (mean survival time, 9.3 months). Tumors that displayed LOH of chromosome 17p were associated with metastatic disease. The prognosis of these tumors was worse only when associated with amplification of c-myc. Three of three patients with LOH of 9q22 relapsed. CONCLUSION In our study, amplification of c-myc was a poor-prognosis marker in PNET. LOH of chromosome 17p was associated with metastatic disease. Molecular analysis of primary tumors using these markers may be useful for stratification of children with PNET in future prospective studies. The other aberrations investigated were not of significant prognostic value, but may provide an entry point for future large-scale molecular studies.
Single Relay Selection Schemes for Broadcast Networks

Achieving the goal of reliably delivering data to all nodes in broadcast wireless networks is very challenging since wireless channels may experience severe variations in signal strength and channel impairments. To mitigate this problem, one or several relays can be used as collaborators to forward the broadcasted signal to other nodes. In this paper, we propose and investigate several single relay selection schemes in broadcast wireless networks using either selective digital relaying or selective analog relaying. The key idea is to classify the nodes in the considered broadcast network into two sets. A set of "reliable" nodes, whose source-node signal-to-noise ratio exceeds a threshold value and a set of "unreliable" nodes gathering the remaining ones. Then, one node among "reliable" nodes is activated as a relay. We derive closed form expressions of the end-to-end bit error probabilities of some proposed single relay selection schemes for selective digital relaying. The data rate loss due to the cooperation is also studied. Analytical results along with simulations prove that compared to the direct transmission, the single relay selection schemes improve significantly the bit error probability performance of the broadcast network.
Effect of Drug Transporter Genes on Cysteine Export and Overproduction in Escherichia coli

ABSTRACT l-Cysteine is an important amino acid in terms of its industrial applications. We previously found a marked production of l-cysteine from glucose in recombinant Escherichia coli cells expressing an altered cysE gene encoding feedback inhibition-insensitive serine acetyltransferase. Also, a lower level of cysteine desulfhydrase (CD) activity, which is involved in l-cysteine degradation, increased l-cysteine productivity in E. coli. The use of an l-cysteine efflux system could be promising for breeding l-cysteine overproducers. In addition to YdeD and YfiK, which have been reported previously as l-cysteine exporter proteins in E. coli, we analyzed the effects of 33 putative drug transporter genes in E. coli on l-cysteine export and overproduction. Overexpression of the acrD, acrEF, bcr, cusA, emrAB, emrKY, ybjYZ, and yojIH genes reversed the growth inhibition of tnaA (the major CD gene)-disrupted E. coli cells by l-cysteine. We also found that overexpression of these eight genes reduces intracellular l-cysteine levels after cultivation in the presence of l-cysteine. Amino acid transport assays showed that Bcr overexpression conferring bicyclomycin and tetracycline resistance specifically promotes l-cysteine export driven by energy derived from the proton gradient. When a tnaA-disrupted E. coli strain expressing the altered cysE gene was transformed with a plasmid carrying the bcr gene, the transformant exhibited more l-cysteine production than cells carrying the vector only. A reporter gene assay suggested that the bcr gene is constitutively expressed at a substantial level. These results indicate that the multidrug transporter Bcr in the major facilitator family is involved in l-cysteine export and overproduction in genetically engineered E. coli cells.
Robust Controller Synthesis for the Attenuation of Non-stationary Sinusoidal Disturbances with Uncertain Frequencies

Abstract Attenuation of sinusoidal disturbances with uncertain and arbitrarily time-varying frequencies is considered. The disturbances are modeled as the outputs of an autonomous exogenous system, whose system matrix depends on some uncertain parameters and is yet skew-symmetric for all admissible parameter values. A procedure is then developed for the synthesis of a linear time-invariant controller that guarantees a desired level of attenuation at steady-state as well as sufficiently fast transient response in the face of all admissible parameter variations. The procedure is based on solving a convex optimization problem in which the variables are subject to a set of linear matrix inequality as well as equality constraints. The order of the controller is equal to the order of the plant plus the order of the exogenous system.
Annexin V binding perturbs the cardiolipin fluidity gradient in isolated mitochondria. Can it affect mitochondrial function?

The phosholipid bilayer fluidity of isolated mitochondria and phospholipid vesicles after calcium-dependent binding of annexin V was studied using EPR spectroscopy. The membranes were probed at different depths by alternatively using cardiolipin, phosphatidylcholine, or phosphatidylethanolamine spin labeled at position C-5 or C-12 or C-16 of the beta acyl chain. Computer-aided spectral titration facilitated observing and quantitating the EPR spectrum from phospholipid spin labels affected by annexin binding, and spectral mobility was calibrated by comparison with standard spectra scanned at various temperatures. In most cases it was found that binding of the protein to the membranes makes the inner bilayer more rigid up to acyl position C-12 than afterward, in agreement with the previously observed effect in SUVs [Megli, F. M. , Selvaggi, M. , Liemann, S. , Quagliariello, E. , and Huber, R. (1998) Biochemistry 37, 10540-10546]. Moreover, in isolated mitochondrial membranes, cardiolipin apparently is more readily affected than the other main phospholipids, while in vesicles made from mitochondrial phospholipids, the different species are affected in essentially the same way. This behavior is consistent with the existence of distinct cardiolipin pools in mitochondria, and with the already advanced hypothesis that these domains are the binding site for annexin V to the isolated organelles [Megli, F. M. , Selvaggi, M. , De Lisi, A. , and Quagliariello, E. (1995) Biochim. Biophys. Acta 1236, 273-278]. Keeping in mind the funcional importance of cardiolipin in the mitochondrial membrane, the question is raised as to whether the observed influence of annexin V binding to this phospholipid and its consequent local fluidity alteration might affect the mitochondrial functionality, at least in vitro.
Resistance to extinction is associated with impaired immediate early gene induction in medial prefrontal cortex and amygdala

Extinction of classical fear conditioning is thought to involve activity‐dependent potentiation of synaptic transmission in the medial prefrontal cortex (mPFC), resulting in the inhibition of amygdala‐dependent fear responses. While many studies have addressed the mechanisms underlying extinction learning, it is unclear what determines whether extinction memory is consolidated or whether spontaneous recovery of the fear response occurs. Here we show, using a combined electrophysiological and immunocytochemical approach, that spontaneous recovery of conditioned fear in mice is associated with a prolonged expression of long‐term depression of synaptic transmission in the mPFC and the failure of induction of the immediate–early genesc‐Fos and zif268 in the mPFC and the basolateral nucleus of the amygdala. This suggests that coordinated activity‐dependent changes in gene expression in the mPFC and the amygdala may underlie the formation of long‐term fear extinction memory.
Personality , Self-esteem predictors of happiness and depression among High School Student in Iran

The aim of this paper was study correlations between personality ,self-esteem and happiness ,depression among 110 boy s student in high school . In all 110 participants completed the Myers-Briggs Type Indicator ,Rosenberg Self Esteem Scale ,Beck Depression Inventory ,and Oxford Happiness Inventory . Results indicated that among of four dimension of personality extraversion(r=0.32,r= 0.45;p<001) and Intuition(r=0.21,r= 0.11;p<0.001) were significantly correlated with happiness and depression . Self-esteem was significantly correlated with happiness and depression (r= 0.57 ,r= -0.42;p<0.01).
The effects of acute doses of standardized Ginkgo biloba extract on memory and psychomotor performance in volunteers

This study investigated the effects of acute doses of Ginkgo biloba extract (GBE) on memory and psychomotor performance in a randomized, double‐blind and placebo controlled 5‐way cross‐over design. Thirty‐one volunteers aged 30–59 years received GBE 150 mg (50 mg t.d.s), GBE 300 mg (100 mg t.d.s.), GBE 120 mg mane and GBE 240 mg mane and placebo for 2 days. Following baseline measures, the medication was administered at 0900 h for the single doses and at 0900, 1500 and 2100 h for the multiple doses. The psychometric test battery was administered pre‐dose (0830 h) and then at frequent intervals until 11 h post dose.
Diagnostic accuracy of serum antibodies to human papillomavirus type 16 early antigens in the detection of human papillomavirus–related oropharyngeal cancer

Because of the current epidemic of human papillomavirus (HPV)–related oropharyngeal cancer (OPC), a screening strategy is urgently needed. The presence of serum antibodies to HPV‐16 early (E) antigens is associated with an increased risk for OPC. The purpose of this study was to evaluate the diagnostic accuracy of antibodies to a panel of HPV‐16 E antigens in screening for OPC.
Searchable encryption through obfuscation and multi-cloud search

In this paper different searchable encryption techniques are studied along with some implementation of searchable encryption in a cloud environment. Symmetric searchable encryption is implemented through trapdoor function to selectively expose keyword for search. A new method of achieving searchable encryption in the random oracle model is proposed through one way indistinguishability obfuscation. Indistinguishability obfuscation is achievable through mimicry function and one way cryptographic hash function. Security of the model is also analyzed with non adaptable indistinguishability security. Using this method an efficient idea of multi-cloud search environment is also proposed. In multi cloud searchable encryption several independent data providers collaborate in a federated way to provide search in their data through different independent cloud service provider revealing only keywords associated with the data.
4 WD Urban Electric Vehicle Motion Studies Based on MIMO Fuzzy Logic Speed Controller

The Next Future Commercialized 4WD Electric Vehicle Future (EV) is designed to minimize the autonomous, the time range of starting and acceleration, in order to improve the vehicle stability and the other dynamic performances for this reason the multi-input multioutput fuzzy logic controller (MIMO-FLC) was presented and implemented in the electrical traction system forming the maximum control process, the advantages of this control structure ( MIMO-FLC) is to give more and more safety and stability for the 4WD comparing with the classical fuzzy controller , The main object of this paper is to show the efficiency of the MIMO-FLC applied on four speeds electric vehicles, the 4WD is powered by four induction motors of 15 kilowatts of each one developing an effort of 338 N. m as global torque so this kind of vehicle consist of two front directing wheels and rear propulsion wheels . The MIMO-FLC control technique is simulated in MATLAB SIMULINK environment. The simulation results proves that the MIMO-FLC method decreases the error speeds of the differential electronic at any curves and mainting the vehicle stability at the curved roads when the transient oscillations are decreased and give good dynamical performance for the vehicle using four induction motors for motion ,it’s clearly that the proposed control present more robustness comparing with the SISO classical ones which have many more problems during the vehicle driving , the results obtained present satisfactory and the vehicle designer must take into consideration the stability effect for the 4WD vehicle moving in road with different topologies using the MIMO-FLC control.
The Relation of Fiscal Decentralization, Regional Finance and Social Justice for the Local Development of Indonesia

This study aims to analyze the relationship between fiscal decentralization, fiscal capacity, financial independence and financial expenditure areas in achieving social justice. In this case, the East Java Province Indonesia is supported by the existence of local government district / municipality became the main object of study during 2010 -2014. The needed data are such as; financial balance data, regional revenue, the amount of local spending and social justice data on people's lives. Data analysis method used is Partial Least Square (PLS). The results provide the conclusion that the implementation of the fiscal decentralization policy gives positive and significant impact on local fiscal capacity. Fiscal capacity gives positive and significant impact on the local financial independence. Local financial independence gives positive and significant impact on the structure of the shopping area. The structure of local government spending has a significant effect on the achievement of social justice areas. In this case, the fiscal capacity and financial independence have no direct impact significantly on the achievement of social justice in the various districts / cities in East Java. In addition, this study also provides important conclusion that there is a significant indirect effect of fiscal decentralization on social justice through local fiscal capacity, local financial independence and the structure of local government spending.
THE ASTRONOMICAL SOCIETY OF THE PACIFIC.

Section 1.1 Principal Office. This corporation’s principal office shall be fixed and located at 390 Ashton Ave, San Francisco, California 94112 The board of directors of this corporation (the “Board”; each member of the Board, a “Director”) may change the location of the principal office. Any such change of location shall be noted by the Secretary on these Bylaws opposite this Section or recorded in an amendment to this Section.
Split soft-decision equalization for wireless channels with large delay spread

A novel split soft-decision equalizer (SSE) with near-optimum performance is proposed for wireless multipath channels with large delay spread. The concept of SSE is significantly different from the traditional notions of the Viterbi algorithm and decision-feedback equalizer. Instead of dealing with received sequence as a combined sequence, it splits the received sequence into its constituent paths. Using an iterative soft-decision algorithm, reliability of the soft decisions on each decomposed element is improved iteratively. The major advantage of SSE is the independence of computation complexity on channel time dispersion. Joint design of SSE with a soft-decision decoder is also considered in this paper. Performance analysis and simulation results show that performance of the proposed algorithm comes very close to that of the logarithmic maximum a posteriori decoder.
The Contradictions and Intersections of Class and Gender in a Global City: Placing Working Women's Lives on the Research Agenda

In this paper we examine the relationships between class and gender in the context of current debates about economic change in Greater London. It is a common contention of the global city thesis that new patterns of inequality and class polarisation are apparent as the expansion of high-status employment brings in its wake rising employment in low-status, poorly paid ‘servicing’ occupations. Whereas urban theorists tend to ignore gender divisions, feminist scholars have argued that new class and income inequalities are opening up between women as growing numbers of highly credentialised women enter full-time, permanent employment and others are restricted to casualised, low-paid work. However, it is also argued that working women's interests coincide because of their continued responsibility for domestic obligations and still-evident gender discrimination in the labour market. In this paper we counterpose these debates, assessing the consequences for income inequality, for patterns of childcare and for work–life balance policies of rising rates of labour-market participation among women in Greater London. We conclude by outlining a new research agenda.