Miracle-dz/UniFAR-FTUs huggingface dataset:
Should We Stay or Should We Go? Analyzing Continuance of Cloud Enterprise Systems

As cloud computing has become a mature technology broadly being adopted by companies across all industries, cloud service providers are increasingly turning their attention to retaining their customers. However, only little research has been conducted on investigating the antecedents of service continuance in an organizational context. To address this gap in research, we carried out a quantitative-empirical study. We developed a conceptual model that builds on previous research on organizational level continuance. We tested this model, using survey data gathered from decision makers of companies which have adopted cloud enterprise systems. The data was analyzed using PLS. The results show that continuance intention can be predicted both by socio-organizational and technology-related factors, explaining 55.9% of the dependent variable’s variance. Besides cloud-specific findings, the study also enhances knowledge in organizational level system continuance as well as its connection to IS success.
Differential interactions of the Mg2+ complexes of chromomycin A3 and mithramycin with poly(dG-dC) x poly(dC-dG) and poly(dG) x poly(dC).

The interaction of the two anticancer antibiotics, chromomycin A3 and mithramycin, with the polynucleotides poly(dG-dC) x poly(dC-dG), representative of B-DNA, and poly(dG) x poly(dC), representative of A-DNA, in the presence of Mg2+ is studied by spectroscopic techniques such as absorbance, fluorescence, and dircular dichroism (CD). The studies were done with both drug x Mg2+ complexes, I and II, having 1:1 and 2:1 stoichiometries with respect to drug and Mg2+, respectively [Aich, P., Sen, R. , & Dasgupta, D. (1992) Biochemistry 31, 2988-2997]. The objective of the present work is 2-fold. First, an attempt is made to understand the structural basis of the ligand-DNA interaction, particularly the role of DNA backbone conformation with its groove size and the accessibility of the 2-amino group in the minor groove of guanosine. Second, the role of the antibiotic saccharide moieties in the association with DNA was studied. For this purpose, the spectroscopic characterization of the binding was done followed by the evaluation of binding parameters and associated thermodynamics. Analysis of the observed thermodynamics for the ligand-DNA interactions in terms of the different structures of the polynucleotides was done. The salient results are as follows. Complex I does not discriminate significantly among the A- and B-forms of DNA when it binds to them in an entropy-driven process. On the other hand, complex II for both drugs recognizes B- and A-forms of DNA in different ways. This observation implies that the sequence specificity shown by this complex is a sequel to the difference in the parameters such as groove size and accessibility of the guanosine amino group. Another important finding is that binding with the same polynucleotide is not comparable for the complex II of the two drugs. It emphasizes the involvement of the sugar moieties, when the drug x Mg2+ complex binds to DNA. The presence of an acetoxy group in the sugars of chromomycin A3 imparts some distinctive specific features of the association of the chromomycin dimer x Mg2+ complex with DNA. Finally, the results are compared with those available from NMR studies of different drug-oligonucleotide complexes under conditions where complex II is the ligand.
Two Shope papillomavirus-associated VX2 carcinoma cell lines with different levels of keratinocyte differentiation and transplantability

Two cell lines, named VX2T and VX2R, were isolated from the transplantable VX2 carcinoma, a wholly anaplastic tumor established from a carcinoma induced by the Shope cottontail rabbit papillomavirus (CRPV) (J.G. Kidd and P. Rous, J. Exp. Med. 71:813-838, 1940). The CRPV genome was found to be maintained and transcribed in both cell lines, as in the VX2 carcinoma. The VX2T cells retained the tumor-producing capacity in the rabbit and the low expression of epidermal keratinocyte differentiation of the VX2 tumor cells. The VX2R cells, although tumorigenic for nude mice, were no longer serially transplantable in the rabbit and expressed differentiated functions of keratinocytes. These data indicate that the anaplastic characteristic and the transplantability of VX2 carcinoma cells to immune competent allogenic hosts may be lost without any detectable modification of the physical state and transcription of the CRPV genome.
“In Flight Catering”: Feeding Critical Care Patients During Aeromedical Evacuation

The benefits of early enteral nutrition are well recognised but may be incompatible with CCAST evacuation due to the risk of micro-aspiration predisposing to pneumonia. A study has been approved by the Surgeon Generals Research Strategy Group designed to quantify the risks of microaspiration during CCAST flights in order to inform DMA policy with regard to feeding critically ill casualties during flight.
The Utilization of Social Media Marketing in Destination Management Organizations

The emerging use of social media fundamentally changes the communication and interaction of societies and organizations. It revolutionizes the way organizations market their products and interact with their customers. Social media acts as an agent of change within the marketing and communication of organizations. In particular, the tourism sector is affected by these disruptive changes. Social media influences and changes the interaction between touristic supply and demand sides fundamentally. These upcoming opportunities and challenges are especially relevant for destination management organizations (DMOs), which try to coordinate and market the intangible and immaterial tourism product of a destination. Social media marketing transforms the classical marketing and communication of these destination management organizations. Nevertheless, destination management organizations are still in an experimental stage of utilizing social media as a marketing tool, as they slowly start to realize the potential benefits of social media as an agent of change. This study investigates how DMOs in alpine regions implement social media marketing in their classical marketing strategies through a quantitative survey. Furthermore, the paper outlines which upcoming challenges and opportunities DMOs face by conducting qualitative interviews with social media managers in the destinations. Finally, implications and recommendations for DMOs are presented to cope with the emerging use of social media as a marketing tool.
Genes and Antibodies

An antibody is a specific globulin which in the semm of an mima1 nfter the induc t ion of a foreign lubt~nce, an antigen ( I ) . Each of the many globulint h specified by i u reaction with a particulnr antigm (2). Our prumt concern is to formulnte n plnwible mechmism for the role of the antigm in evoking Iaqe amounts of n s p cific complementary globulin. An important element of any theory of antibody formation is iu interpretation of selfrecognition, the mc.N by which nn organism dircriminatu iu own constituent( from the foreign sulntnnces which are valid stimuli of the immune re-
The GpsB files: the truth is out there

Peptidoglycan (PG), an essential stress‐bearing component of the bacterial cell wall, is synthesised by penicillin binding proteins (PBPs). PG synthesis at the cell division septum is necessary for constructing new poles of progeny cells, and cells cannot elongate without inserting new PG in the side‐wall. The cell division regulator GpsB appears to co‐ordinate PG synthesis at the septum during division and at the side‐wall during elongation in rod‐shaped and ovococcoid Gram‐positive bacteria. How the control over PG synthesis is exerted is unknown. In this issue of Molecular Microbiology, Rued et al. show that in pneumococci GpsB forms complexes with PBP2a and PBP2b, and that deletion or depletion of GpsB prevents closure of the septal ring that in itself is PBP2x‐dependent. Loss of GpsB can be suppressed by spontaneous mutations, including within the gene encoding the only PP2C Ser/Thr phosphatase in Streptococcus pneumoniae, indicating that GpsB plays a key – but unknown – role in protein phosphorylation in pneumococci. Rued et al. combine phenotypic and genotypic analyses of mutant strains that suggest discrepancies in the literature concerning GpsB might have arisen from accumulation of unidentified suppressors, highlighting the importance and power of strain validation and whole genome sequencing in this context.
A new PK equivalence test for a bridging study

ABSTRACT In a bridging study, the plasma drug concentration–time curve is generally used to assess bioequivalence between the two formulations. Selected pharmacokinetic (PK) parameters including the area under the concentration–time curve, the maximum plasma concentration or peak exposure (Cmax), and drug half-life (T1/2) are compared to ensure comparable bioavailability of the two formulations. Comparability in these PK parameters, however, does not necessarily imply equivalence of the entire concentration–time profile. In this article, we propose an alternative metric of equivalence based on the maximum difference between PK profiles of the two formulations. A test procedure based on Bayesian analysis and accounting for uncertainties in model parameters is developed. Through both theoretical derivation and empirical simulation, it is shown that the new method provides better control over consumer’s risk.
Temporal cortex dopamine D2/3 receptor binding in major depression

The aim of this study was to assess the dopamine function of the temporal cortex in major depressive disorder using [123I]epidepride to image D2/3 receptor binding sites. Ten major depressives and 10 healthy controls were selected from a general population sample for single‐photon emission computed tomography imaging. Among the major depressives there was a strong bilateral correlation between the scores on the 21‐item Hamilton Depression Rating Scale and D2/3 receptor binding. Dopaminergic abnormalities may be present in the temporal cortices of major depressives.
Accessing the Black Box of Microbial Diversity and Ecophysiology: Recent Advances Through Polyphasic Experiments

The microbial ecology of a range of anaerobic biological assemblages (granular sludge) from full- and laboratory-scale wastewater treatment bioreactors, and of crop-growing and peat soils, was determined using a variety of 16S rRNA gene-based techniques, including clone library, terminal restriction fragment length polymorphism (TRFLP) and denaturing gradient gel electrophoresis (DGGE) analyses. Fluorescent in situ hybridization (FISH) using 16S rRNA gene-targeted probes was employed to complete a “full-cycle rRNA approach” with selected biomass. Genetic fingerprinting (TRFLP and DGGE) was effectively used to elucidate community structure-crop relationships, and to detect and monitor trends in bioreactor sludge and specific enrichment cultures of peat soil. Greater diversity was resolved within bacterial than within archaeal communities, and unexpected reservoirs of uncultured Crenarchaeota were detected in sludge granules. Advanced radiotracer incubations and micro-beta imaging were employed in conjunction with FISH to elucidate the eco-functionalism of these organisms. Crenarchaeota clusters were identified in close associated with methanogenic Archaea and both were localised with acetate uptake in biofilm structure.
The impact of knowledge creation on employee engagement from the perspectives of exploitation and exploration

The main purpose of this study is to assess the impacts of knowledge creation on employee engagement from both exploration and exploitation perspectives. After identifying that knowledge creation could facilitate employee engagement from a literature review, the study proposed a model of knowledge creation toward employee engagement. A survey questionnaire was developed accordingly. With a stratified sampling method, data were collected from the academic staff of one public and one private university located in Malaysia. A total of 200 academic staff members participated in this study. Linear regression analysis was performed to test how knowledge creation (i.e., exploration and exploitation) affects employee engagement. Statistical results show that the exploration and exploitation of knowledge creation positively and significantly affect employee engagement. The proposed model could aid universities in utilising knowledge creation practices to engage employees so as to attain and sustain competitive advantage.
Method for the quantitative assessment of contrast agent uptake in dynamic contrast‐enhanced MRI

In previous papers relative signal intensity increase was used as a quantitative assessment parameter for contrast uptake in contrastenhanced MRI. However, relative signal intensity increase does not only reflect contrast uptake but depends also on tissue parameters (native T1 relaxation time) and sequence parameters (repetition time and flip angle); thus, the contrast uptake cannot be assessed accurately using relative signal intensity increase. Based on an analysis of the contrast behavior of spoiled gradient echo sequences, a method is described in this paper that overcomes the limitations of relative signal intensity increase measurement. A parameter, called “enhancement factor” (EF) is introduced that approximates differential T1 relaxation rate. The enhancement factor scales linearly with contrast uptake and is independent of tissue and sequence parameters. The additional measurement time involved in determining the enhancement factor is less than 1 min and computation is straightforward. The practicality of the new method was confirmed by phantom measurements using T1‐weighted and proton density‐weighted spoiled gradient echo sequences (FLASH‐2D). Enhancing tissues were simulated by water phantoms doped with increasing concentrations of Gd‐DTPA.
Homo- and Heterooligomeric SNARE Complexes Studied by Site-directed Spin Labeling*

SNARE (soluble NSF acceptor protein receptor) proteins are thought to mediate membrane fusion by assembling into heterooligomeric complexes that connect the fusing membranes and initiate the fusion reaction. Here we used site-directed spin labeling to map conformational changes that occur upon homo- and heterooligomeric complex formation of neuronal SNARE proteins. We found that the soluble domains of synaptobrevin, SNAP-25, and syntaxin 1 are unstructured. At higher concentrations, the SNARE motif of syntaxin 1 forms homooligomeric helical bundles with at least some of the α-helices aligned in parallel. In the assembled SNARE complex, mapping of thirty side chain positions yielded spectra which are in good agreement with the recently published crystal structure. The loop region of SNAP-25 that connects the two SNARE motifs is largely unstructured. C-terminal truncation of synaptobrevin resulted in complexes that are completely folded N-terminal of the truncation but become unstructured at the C-terminal end. The binary complex of syntaxin and SNAP-25 consists of a parallel four helix-bundle with properties resembling that of the ternary complex.
Imaging and mapping protein-binding sites on DNA regulatory regions with atomic force microscopy.

Regulation of gene expression is fundamental in biological systems. A systematic search for protein binding sites in gene promoters has been done in recent years. Biochemical techniques are easy and reliable when analysing protein interactions with short pieces of DNA, but are difficult and tedious when long pieces of DNA have to be analysed. Here we propose AFM as a reliable and easy technique for identifying protein interaction sites in long DNA molecules like gene promoters. We support this idea using a well-known model: the interaction of the Pho4 protein with the PHO5 gene promoter. We have also applied the technique to demonstrate that Mig1 protein binds to two motifs in the promoter of HXK2 gene. Our results allow us to define Mig1p as a new factor probably contributing to the carbon source-dependent transcription regulation of HXK2 gene.
Novel Evidence for the Bilingual Advantage: Effects of Language Control on Executive Function in Balanced and Unbalanced Dual-Language Users

Bilinguals’ need to monitor and inhibit non-relevant languages over a relevant one confers advantage in cognitive control. No studies have demonstrated that the dual-language control process directly contributes to the bilingual cognitive advantage. We utilized a novel language control manipulation paradigm where 83 English-Chinese bilingual adults completed a reading and comprehension task in either singlelanguage (low-language-control) or dual-language (highlanguage-control) prior to performing nonverbal executive control tasks (Stroop, task-switching, and n-back). Results showed that language control had significant effects on subsequent cognitive performance, depending on whether the participants were regular dual language users or not. In the dual-language condition, but not the single-language condition, participants who used both languages regularly demonstrated a smaller mixing cost in task-switching and a greater sensitivity in n-back detection compared to participants who did not. This suggests that dual language control utilizes similar resources as executive function and frequent dual language use enhances this resource.
Sur la commande robuste et lpv de systemes a parametres lentement variables

Cette these s'inscrit dans le theme des travaux relatifs a la synthese de lois de commandes robustes de systemes lineaires incertains et a parametres variants, dans le but de conferer a ces systemes dynamiques un certain niveau de performance. On considere des modeles lineaires decrits dans l'espace d'etat avec des incertitudes de type polytopique convexe. Cette representation des systemes dynamiques permet de traiter des problemes de commande robuste bases sur la notion de stabilisabilite quadratique, ou une seule fonction de lyapunov et un seul compensateur sont utilises pour garantir les performances et la stabilite du systeme etudie. Cette approche conduit a des conditions de type bilineaire, et, afin de resoudre de tels problemes, un algorithme dit par decomposition croisee conduit a la resolution d'une sequence de problemes lineaires pour lesquels l'utilisation de l'outil lmi est possible. Afin de reduire le conservatisme de l'approche precedente, est introduite la notion de commande lpv, ou est faite la synthese d'un compensateur variant dans le temps ayant la meme structure que le systeme. Cette approche conduit a des contraintes de type lmis facilement implementables. Ces differentes approches sont illustrees sur le modele d'un systeme reel : un derouleur de bande magnetique.
Effectiveness of macroprudential policies in Central and Eastern European countries

This paper extends the available datasets on the use of macroprudential policies in CEE countries, and provides an econometric assessment of the effectiveness of these policies in mitigating financial stability risks associated with excessive credit growth before the global financial crisis. The model results imply that macroprudential policies were more effective in slowing credit to households than credit to the non-financial corporate sector, mainly because the latter had access to nonbank and cross-border credit in addition to domestic bank credit.
Prevalence and molecular characterisation of Giardia duodenalis in calves with diarrhoea

The aim of this study was to investigate the prevalence of Giardia duodenalis in diarrhoeal faeces from calves that were reared in Korea using PCR and ELISA. Diarrhoeal faecal samples were collected from 590 calves (<3 months old) throughout the country from November 2013 through March 2015. Data were analysed according to the region of collection, season, and type of diarrhoea. Of the 590 collected faecal samples, 77 (13.1 per cent) and 78 (13.2 per cent; true prevalence 9.1 per cent) tested positive by PCR and ELISA, respectively. The results from both testing methods were in agreement in 501 (84.9 per cent) samples, with a κ value of 0.34, which suggests fair agreement. The PCR results showed a higher prevalence in summer and in cases of haemorrhagic diarrhoea (P<0.05), while the ELISA results showed a lower prevalence in cases of pasty diarrhoea (P<0.05). Phylogenetic analysis of the β-giardin gene sequences of G duodenalis showed that the sequences detected in this study belonged to assemblage E, which is specific to cattle and livestock. The higher prevalence of giardiasis in calves with haemorrhagic diarrhoea found in this study differed from previous studies. Therefore, researchers and veterinarians should be aware of the possible involvement of giardiasis in haemorrhagic diarrhoea.
Reliable messaging to millions of users with migratorydata

Web-based notification services are used by a large range of businesses to selectively distribute live updates to customers, following the publish/subscribe (pub/sub) model. Typical deployments can involve millions of subscribers expecting ordering and delivery guarantees together with low latencies. Notification services must be vertically and horizontally scalable, and adopt replication to provide a reliable service. We report our experience building and operating MigratoryData, a highly-scalable notification service. We discuss the typical requirements of MigratoryData customers, and describe the architecture and design of the service, focusing on scalability and fault tolerance. Our evaluation demonstrates the ability of MigratoryData to handle millions of concurrent connections and support a reliable notification service despite server failures and network disconnections.
Towards Software Mass Customization for Business Collaboration

Supply chains are being transformed into agile networks in response the increasing volatility of business environments. The dynamic nature of business collaboration in such networks imposes stringent demands on the enabling software systems. This paper proposes the application of a mass customization approach to meet the great demands on software systems for business collaboration in agile business networks. We first introduce the concept of mass customization and define requirements for software mass customization. Next, the paper presents a Future Internet platform - called FIspace - for business to business collaboration that is currently being developed within Europe's Future Internet Public-Private Partnership program (FI-PPP) and that aims to provide an environment for software mass customization for business collaboration in the agriculture, food and logistics sectors.
Aldehyde dehydrogenase 2 inhibits inflammatory response and regulates atherosclerotic plaque

Previous studies demonstrated that aldehyde dehydrogenase 2 (ALDH2) rs671 polymorphism, which eliminates ALDH2 activity down to 1%-6%, is a susceptibility gene for coronary disease. Here we investigated the underlying mechanisms based on our prior clinical and experimental studies. Male apoE−/− mice were transfected with GFP, ALDH2-overexpression and ALDH2-RNAi lentivirus respectively (n=20 each) after constrictive collars were placed around the right common carotid arteries. Consequently, ALDH2 gene silencing led to an increased en face plaque area, more unstable plaque with heavier accumulation of lipids, more macrophages, less smooth muscle cells and collagen, which were associated with aggravated inflammation. However, ALDH2 overexpression displayed opposing effects. We also found that ALDH2 activity decreased in atherosclerotic plaques of human and aged apoE−/− mice. Moreover, in vitro experiments with human umbilical vein endothelial cells further illustrated that, inhibition of ALDH2 activity resulted in elevating inflammatory molecules, an increase of nuclear translocation of NF-κB, and enhanced phosphorylation of NF-κB p65, AP-1 c-Jun, Jun-N terminal kinase and p38 MAPK, while ALDH2 activation could trigger contrary effects. These findings suggested that ALDH2 can influence plaque development and vulnerability, and inflammation via MAPK, NF-κB and AP-1 signaling pathways.