Miracle-dz/UniFAR-FTUs huggingface dataset:
Specific Binding of the Karyopherin Kap 121 p to a Subunit of the Nuclear Pore Complex Containing Nup 53 p , Nup 59 p , and Nup 170 p

We have identified a specific karyopherin docking complex within the yeast nuclear pore complex (NPC) that contains two novel, structurally related nucleoporins, Nup53p and Nup59p, and the NPC core protein Nup170p. This complex was affinity purified from cells expressing a functional Nup53p–protein A chimera. The localization of Nup53p, Nup59p, and Nup170p within the NPC by immunoelectron microscopy suggests that the Nup53p-containing complex is positioned on both the cytoplasmic and nucleoplasmic faces of the NPC core. In association with the isolated complex, we have also identified the nuclear transport factor Kap121p (Pse1p). Using in vitro binding assays, we showed that each of the nucleoporins interacts with one another. However, the association of Kap121p with the complex is mediated by its interaction with Nup53p. Moreover, Kap121p is the only b -type karyopherin that binds Nup53p suggesting that Nup53p acts as a specific Kap121p docking site. Kap121p can be released from Nup53p by the GTP bound form of the small GTPase Ran. The physiological relevance of the interaction between Nup53p and Kap121p was further underscored by the observation that NUP53 mutations alter the subcellular distribution of Kap121p and the Kap121pmediated import of a ribosomal L25 reporter protein. Interestingly, Nup53p is specifically phosphorylated during mitosis. This phenomenon is correlated with a transient decrease in perinuclear-associated Kap121p.
Global changes in gene expression related to antibiotic synthesis in Streptomyces hygroscopicus

Two‐dimensional gel electrophoresis was used to follow low changes in gene expression associated with antibiotic (bialaphos) biosynthesis in Streptomyces hygroscopicus. Cultures were pulse‐labelled with [35S]‐methionine before, during, and after the switch from primary to secondary metabolism in order to compare kinetic profiles of bialaphos (antibiotic) production (bap) genes during this metabolic transition. Separation of gene products on two‐dimensional gels revealed that 27 were dependent on brpA for optimal expression and were activated as the culture approached stationary phase. Genes which encoded 10 brp4‐dependent proteins were mapped to a 10 kb Sstl fragment of the 35 kb bap gene cluster by expressing them in Streptomyces lividans using the thiostrepton‐inducible tipA promoter. N‐terminal amino acid sequences of two brpA‐dependent proteins, obtained by direct microsequencing of protein spots excised from two‐dimensional gels, identified them as gene products mapping to the same region and involved in secondary metabolic conversions of the bap pathway. The kinetics of synthesis of 16 brpA‐dependent gene products were characterized using QUEST computer software. Cluster analysis performed on the kinetics of synthesis of 346 of the most highly expressed gene products of HP5‐29, including 16 brpA‐dependent ones, identified 75 families having distinct patterns of expression. Many brpA‐dependent proteins were clustered together; 10 were found in one kinetic family. These kinetic families also included brpA‐independent gene products perhaps subject to similar regulatory mechanisms and thus possibly involved in bialaphos biosynthesis. The activation/derepression of bap expression took place as cultures approached stationary phase and was temporally related to synthesis of ppGpp.
An Evaluation of the Feasibility and Safety of a Home-Based Telemental Health Treatment for Posttraumatic Stress in the U.S. Military.

BACKGROUND Although home-based telemental health options have the potential to greatly expand the range of services available to U.S. military service members, there remains a need to demonstrate that home-based care is technically feasible, safe, and effective and meets the military health system's standards of care before widespread implementation can be achieved. The purpose of this preliminary study was to evaluate the feasibility and safety of providing U.S. military service members with a behavioral health treatment delivered directly to the home using videoconferencing. MATERIALS AND METHODS Ten previously deployed soldiers volunteered to complete eight sessions of a novel behavioral activation treatment for posttraumatic stress disorder. The primary clinical outcomes assessed included symptoms of posttraumatic stress and depression. Patient safety data and attitudes about seeking mental health services, treatment satisfaction, treatment adherence, and treatment compliance were also assessed. RESULTS Clinically significant reductions in posttraumatic stress symptom severity and depression symptoms were observed. Soldiers indicated high levels of satisfaction with the treatment, and there were no adverse events requiring activation of emergency safety procedures. Technical problems associated with the network were observed but successfully mitigated. CONCLUSIONS The results provide initial support for the feasibility and safety of telemental health treatments delivered by videoconferencing to the homes of soldiers. The optimal technical infrastructure needs to be determined to support expansion of synchronous videoconferencing capabilities to the home. The findings provide preliminary evidence of the feasibility, safety, and high user satisfaction with home-based telemental health in the military setting.
Assessing lifetime diet: reproducibility of a self-administered, non-quantitative FFQ

Abstract Objective To demonstrate test–retest reliability (reproducibility) of a new self-administered lifetime diet questionnaire, with a focus on foods relevant to cognitive health in older age. Design The reproducibility of dietary recall over four or five life periods was assessed by administering the questionnaire at two time points to an older cohort. The period between questionnaire administrations was 7 weeks. Polychoric correlations measured the association between recall at time 1 and time 2 and the weighted κ statistic measured the level of recall agreement for food groups across the two administrations of the questionnaire. Setting Adelaide, South Australia. Subjects Fifty-two cognitively healthy, older-age, community-dwelling adults completed the Lifetime Diet Questionnaire; mean age 81·8 (sd 4·4) years, range 70–90 years. Results The questionnaire showed very good reproducibility in this sample with a mean polychoric correlation coefficient of 0·81 between administration at time 1 and time 2, and an average weighted κ of 0·49 for the level of recall agreement between food groups. Conclusions The demonstrated reliability of this lifetime diet questionnaire makes it a useful tool to assess potential relationships between long-term dietary intake and later-age cognitive outcomes.
ANÁLISIS PSICOLÓGICO DEL EMPRENDIMIENTO EN ESTUDIANTES UNIVERSITARIOS: MEDICIÓN, RELACIONES Y PREDICCIÓN

El presente articulo aborda el tema de la actitud emprendedora desde una triple perspectiva. En primer lugar, se exploraron las posibilidades de medicion de dicha actitud; para ello se adapto, a la poblacion universitaria boliviana, una escala desarrollada en otro contexto cultural llevandose a cabo de manera exitosa su prueba psicometrica. En segundo lugar, el estudio reporta una serie de relaciones entre la actitud emprendedora y otras variables psicologicas tales como la disposicion a cambiar, la ego-resiliencia, la auto-eficacia emocional y los valores de apertura al cambio. Los resultados muestran correlaciones significativas entre alta actitud emprendedora y todas las variables mencionadas. Por el contrario la baja actitud emprendedora no exhibio correlaciones relevantes con ninguna de tales variables. En tercer lugar, el analisis de regresion multiple permitio vislumbrar un modelo para la prediccion de la actitud emprendedora a partir de la disposicion a cambiar, de la ego-resiliencia y de los valores de autodireccion y logro. Finalmente se discuten los resultados generales a la luz de la necesidad de generar condiciones para la formacion de la actitud y conducta emprendedoras.
Live Collaborative Social-Media Video Timelines

In this paper, we propose a collaborative system to let users share their own videos and interact among themselves to collaboratively do a video coverage of live events. Our intention is to motivate users to make positive contributions to the comprehensiveness of available videos about that event. To achieve this we propose a collaborative video framework, named LiveTime, allowing users to shared information timelines of real-world events. With this solution we offer collaboration features that go beyond existing systems like Youtube and Vimeo. The paper describes the rational and main concepts, the implementation and the results of a preliminary user study.
Molecular cloning, in-silico characterization and functional validation of monodehydroascorbate reductase gene in Eleusine coracana

Ascorbic acid is a ubiquitous water soluble antioxidant that plays a critical role in plant growth and environmental stress tolerance. It acts as a free radical scavenger as well as a source of reducing power for several cellular processes. Because of its pivotal role in regulating plant growth under optimal as well as sub-optimal conditions, it becomes obligatory for plants to maintain a pool of reduced ascorbic acid. Several cellular processes help in maintaining the reduced ascorbic acid pool, by regulating its synthesis and regeneration processes. Current study demonstrates that monodehydroascorbate reductase is an important enzyme responsible for maintaining the reduced ascorbate pool, by optimizing the recycling of oxidized ascorbate. Cloning and functional characterization of this important stress inducible gene is of great significance for its imperative use in plant stress management. Therefore, we have cloned and functionally validated the role of monodehydroascorbate reductase gene (mdar) from a drought tolerant variety of Eleusine coracana. The cloned Ecmdar gene comprises of 1437bp CDS, encoding a 478 amino acid long polypeptide. The active site analysis showed presence of conserved Tyr348 residue, facilitating the catalytic activity in electron transfer mechanism. qPCR expression profiling of Ecmdar under stress indicated that it is an early responsive gene. The analysis of Ecmdar overexpressing Arabidopsis transgenic lines suggests that monodehydroascorbate reductase acts as a key stress regulator by modulating the activity of antioxidant enzymes to strengthen the ROS scavenging ability and maintains ROS homeostasis. Thus, it is evident that Ecmdar is an important gene for cellular homeostasis and its over-expression could be successfully used to strengthen stress tolerance in crop plants.
Spatial and temporal factors in auditory saltation.

This report describes three experiments on auditory saltation, studied with click stimuli presented at interclick intervals (ICIs) from 30 to 240 ms. In experiment 1, subjects rated the strength of the saltation illusion evoked by trains of six monaural clicks (i.e., three presented to one ear followed by three to the other ear), and six dichotic clicks on which were imposed either a 500-micros interaural time difference or a 9-dB interaural level difference (ILD). The interaural disparity of theclicks was reversed at the midpoint of the train. Subjects reported equivalent strengths of saltation for the dichotic clicks, but weaker saltation for the monaural ones. These data indicated that saltation is supported by dichotic clicks, regardless of the stimulus manipulation used to generate the lateralized images. In experiment 2, subjects rated the strength of the saltation evoked by six click trains lateralized, in separate trials, by ILDs ranging from 9 to 30 dB, and by a train of monaural clicks. In all ILD conditions, the level of the clicks at the "quiet" ear was above click-detection threshold when presented monaurally. Saltation weakened with increasing ILD, and approximated that seen with monaural clicks when the ILD was 30 dB. These data indicated that for the range of ICIs used here, saltation weakened as the stimuli became more strongly lateralized. In experiment 3, the number of dichotic clicks preceding the disparity reversal was, in separate trials, varied from 3 to 10, and subjects were asked to detect the presence of spatial stationarity in the click train. By plotting the subjective ratings as a function of the length of the leading click train, it was shown that the temporal window within which the saltation effect operates varies between listeners, but is usually less than about 350-400 ms.
Développement modulaire de théories et gestion de l'espace de nom pour l'assistant de preuve Coq. (Modular development of theories and name-space management for the Coq proof assistant)

Proof assistants offer a formal framework for formalizing and mechanically checking mathematical knowledge. Moreover, due to the numerous applications that follow from formal methods, the scientifc production being formalized and verified by such tools is constantly growing. In that context, the organization and the classification of this knowledge does not have to be neglected. Coq is a proof assistant well-suited for program certification and mathematical formalization, and for seven years now it has featured a module system that helps users in their development processes. Modules provide a way to represent theories and offer a namespace management that is crucial for large developments. In this dissertation, we advance the module system of Coq by putting the emphasis on the two latter aspects. We propose to unify both module implementation and module type into a single notion of structure, and to split our module system in two parts. We have, on one hand, a namespace system that is able to define extensible naming scopes and to deal with renaming, and on the other hand a structure system that describes how to combine and to form structures. We define a new merge operator that, given two structures, builds the resulting structure by unifying components of the former two. In that dual system, a module is the association of a sub-namespace and a pair of structures, it acts as concrete declared theory. Furthermore, we adopt an applicative semantic for higher-order functors that allows a precise propagation of information. We show that this module system is a conservative extension of the underlying base language of Coq and we present the on-going implementation.
La violencia doméstica

Ensayo sobre la violencia domestica que proporciona una perspectiva global de esta problematica, presentando tambien estudios especificos de la ciudad de Guadalajara. Este tipo de agresion dentro de la familia se encuentra en todas las clases sociales, areas geograficas y profesiones; sin embargo, se caracteriza por su calidad de invisible, los golpes de los esposos a las mujeres son tomados como peleas domesticas que deben resolverse en privado. Se proporcionan cifras sobre la violencia domestica en distintos paises asi como sus legislaciones y sanciones, o la falta de ellas; finalmente se habla de las alternativas que podrian dar respuesta a este problema, como los refugios para mujeres golpeadas o la educacion de los ninos en el respeto a los demas.
Attitude Determination Using Two Vector Measurements

Many spacecraft attitude determination methods use exactly two vector measurements. The two vectors are typically the unit vector to the Sun and the Earth's magnetic field vector for coarse "sun-mag" attitude determination or unit vectors to two stars tracked by two star trackers for fine attitude determination. TRIAD, the earliest published algorithm for determining spacecraft attitude from two vector measurements, has been widely used in both ground-based and onboard attitude determination. Later attitude determination methods have been based on Wahba's optimality criterion for n arbitrarily weighted observations. The solution of Wahba's problem is somewhat difficult in the general case, but there is a simple closed-form solution in the two-observation case. This solution reduces to the TRIAD solution for certain choices of measurement weights. This paper presents and compares these algorithms as well as sub-optimal algorithms proposed by Bar-Itzhack, Harman, and Reynolds. Some new results will be presented, but the paper is primarily a review and tutorial.
Low fitness is associated with exercise abnormalities among asymptomatic firefighters.

BACKGROUND Low cardiorespiratory fitness (CRF) has been repeatedly linked to cardiovascular morbidity and mortality, while higher CRF levels are protective. This relationship is likely to be highly relevant in firefighters, who have increased risk of cardiovascular disease (CVD) mortality during strenuous emergencies, which can require prolonged periods of near-maximal heart rates (HR) and high workloads. Abnormalities during maximal stress testing could mark future CVD risk during strenuous duties. AIMS To determine if low CRF among asymptomatic firefighters is associated with higher risk of electrocardiographic (ECG) and autonomic abnormalities during maximal exercise stress testing and recovery. METHODS Male career firefighters completed a maximal stress test exercising to volitional exhaustion (mean maximal age-predicted HR achieved 98%, standard deviation (SD) = 6.5). CRF was measured as maximal metabolic equivalents (METS) achieved. Abnormal exercise tests included the following: abnormal HR recovery; chronotropic insufficiency; exaggerated blood pressure response; and ECG abnormalities. The relationship of CRF to stress testing abnormalities was analysed using peak METS categories and peak METS as a continuous variable after adjusting for age, body mass index (BMI) and metabolic syndrome (MetSyn). RESULTS There were 1149 study participants. CRF was inversely associated with the risk of both ECG and autonomic exercise testing abnormalities before and after adjustment for age, BMI and MetSyn. CONCLUSIONS Firefighters with lower CRF are significantly more prone to exhibit abnormal stress test parameters, which may indicate higher future risk of cardiovascular events. As such, firefighters with low CRF (≤ 12 METS) should receive cardiovascular risk reduction, including efforts to improve their CRF.
A GIS-Based Accessibility Modeling Process for Estimating Transit Travel Demand

Transit planning requires forecasting travel demand. In the aggregate transport modeling system, the forecasting is accomplished through a four-stage process, in which transit demand is separated from other modes during modal split. Transit travel demand may also be forecasted at a less aggregate level with other methods, such as regression and accessibility models. These disaggregate models measure transit demand based on walking distance to transit stops. This paper presents a pragmatic GIS-based accessibility modeling approach to transit demand forecasting. Principles and data requirements of accessibility models are described. Based on appropriate data model in GIS, an implementation framework is developed for applying the accessibility models. KeywordsGIS, transit travel demand, accessibility, bus stop, process
Vertical distribution of Calanus spp. and Metridia longa at four Arctic locations

Abstract We investigated the vertical distribution of Calanus finmarchicus, C. glacialis, C. hyperboreus and Metridia longa at four locations around the archipelago of Svalbard in autumn. The older and larger copepodites of Calanus spp. were generally located deeper in the water column. Differences in vertical distribution between stations partly reflected a south–north gradient in developmental progress with higher abundance of older stages in the southern locations. The C. finmarchicus and M. longa observations were consistent with the hypothesis that the developmental stages distributed according to certain preferences for light intensity, and different optical properties at the four locations are likely to have affected the vertical distributions. Diel vertical migration was only observed for older developmental stages of M. longa while young stages of M. longa remained in deep waters both day and night. A mortality index indicated that non-migrating Calanus spp. suffered higher mortality than migrating M. longa.
Ca2+-activated chloride channel activity during Ca2+ alternans in ventricular myocytes

ABSTRACT Cardiac alternans, defined beat-to-beat alternations in contraction, action potential (AP) morphology or cytosolic Ca transient (CaT) amplitude, is a high risk indicator for cardiac arrhythmias. We investigated mechanisms of cardiac alternans in single rabbit ventricular myocytes. CaTs were monitored simultaneously with membrane currents or APs recorded with the patch clamp technique. A strong correlation between beat-to-beat alternations of AP morphology and CaT alternans was observed. During CaT alternans application of voltage clamp protocols in form of pre-recorded APs revealed a prominent Ca2+-dependent membrane current consisting of a large outward component coinciding with AP phases 1 and 2, followed by an inward current during AP repolarization. Approximately 85% of the initial outward current was blocked by Cl− channel blocker DIDS or lowering external Cl− concentration identifying it as a Ca2+-activated Cl− current (ICaCC). The data suggest that ICaCC plays a critical role in shaping beat-to-beat alternations in AP morphology during alternans.
Molecular Characterization of Saccharomyces cerevisiae Δ3,Δ2-Enoyl-CoA Isomerase*

We report here the identification of theSaccharomyces cerevisiae peroxisomal Δ3,Δ2-enoyl-CoA isomerase, an enzyme that is essential for the β-oxidation of unsaturated fatty acids. The yeast gene YLR284C was identified in an in silico screen for genes that contain an oleate response element, a transcription factor-binding site common to most fatty acid-induced genes. Growth on oleic acid resulted in a significant increase inYLR284C mRNA, demonstrating that it is indeed an oleate-induced gene. The deduced product of YLR284Ccontains a type 1 peroxisomal targeting signal-like sequence at its C terminus and localizes to the peroxisome in aPEX8-dependent manner. Removal ofYLR284C from the S. cerevisiae genome eliminated growth on oleic acid, but had no effect on peroxisome biogenesis, indicating a role for YLR284C in fatty acid metabolism. Cells lacking YLR284C had no detectable Δ3,Δ2-enoyl-CoA isomerase activity, and a bacterially expressed form of this protein catalyzed the isomerization of 3-cis-octenoyl-CoA to 2-trans-octenoyl-CoA with a specific activity of 16 units/mg. We conclude thatYLR284C encodes the yeast peroxisomal Δ3,Δ2-enoyl-CoA isomerase and propose a new name, ECI1, to reflect its enoyl-CoA isomerase activity.