Miracle-dz/UniFAR-FTUs huggingface dataset:
PERSPECTIVE: THE PACE OF MODERN LIFE: MEASURING RATES OF CONTEMPORARY MICROEVOLUTION

We evaluate methods for measuring and specifying rates of microevolution in the wild, with particular regard to studies of contemporary, often deemed “rapid,” evolution. A considerable amount of ambiguity and inconsistency persists within the field, and we provide a number of suggestions that should improve study design, inference, and clarity of presentation. (1) Some studies measure change over time within a population (allochronic) and others measure the difference between two populations that had a common ancestor in the past (synchronic). Allochronic studies can be used to estimate rates of “evolution,” whereas synchronic studies more appropriately estimate rates of “divergence. ” Rates of divergence may range from a small fraction to many times the actual evolutionary rates in the component populations. (2) Some studies measure change using individuals captured from the wild, whereas others measure differences after rearing in a common environment. The first type of study can be used to specify “phenotypic” rates and the later “genetic” rates. (3) The most commonly used evolutionary rate metric, the darwin, has a number of theoretical shortcomings. Studies of microevolution would benefit from specifying rates in standard deviations per generation, the haldane. (4) Evolutionary rates are typically specified without an indication of their precision. Readily available methods for specifying confidence intervals and statistical significance (regression, bootstrapping, randomization) should be implemented. (5) Microevolutionists should strive to accumulate time series, which can reveal temporal shifts in the rate of evolution and can be used to identify evolutionary patterns. (6) Evolutionary rates provide a convenient way to compare the tempo of evolution across studies, traits, taxa, and time scales, but such comparisons are subject to varying degrees of confidence. Comparisons across different time scales are particularly tenuous. (7) A number of multivariate rate measures exist, but considerable theoretical development is required before their utility can be determined. We encourage the continued investigation of evolutionary rates because the information they provide is relevant to a wide range of theoretical and practical issues.
Construction and Use of a Transposon for Identification of Essential Genes in Mycobacteria

The continuing emergence of multi-drug resistant Mycobacterium tuberculosis is threatening the ability to treat tuberculosis (TB) worldwide. The development of new anti-TB drugs requires new approaches and new drug targets. In this study, a mariner-based transposon, TnQuoVadis, was constructed to identify essential genes as potential drug targets. This transposon has an outward-facing anhydrotetracycline (ATc)-inducible promoter at each end. A mutant with TnQuoVadis inserted upstream of an essential gene may display normal growth in the presence of ATc, but exhibit no growth or severely diminished growth in the absence of ATc. TnQuoVadis was placed onto a vector with a temperature sensitive replication origin for more efficient mutagenesis of mycobacteria. In a preliminary genetic screen using the model organism Mycobacterium smegmatis, 13 mutants with ATc-dependent growth were identified. Identification of the insertion sites by cloning and sequencing indicated that there were nine genetic loci containing transposon insertions upstream of essential gene candidates in M. smegmatis. Further analysis of these genes indicated that many were previously known essential in both M. smegmatis and M. tuberculosis. These results demonstrate that TnQuoVadis and its delivery system can be utilized for the identification of essential genes in mycobacteria.
An Axiomatic Approach to Proportionality between Matrices Author ( s ) :

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. An Axiomatic Approach to Proportionality between Matrices Michel Balinski, Gabrielle Demange
evidence-based recommendations Management of opioid-induced nausea and vomiting in cancer patients : systematic review and

The objectives were to review the existing literature on management of opioid-induced nausea and vomiting in cancer patients and summarize the findings into evidence-based recommendations. Systematic searches of MEDLINE, EMBASE and the Cochrane Central Register of Controlled Trials were performed, using free text and MeSH/EMTREE search terms. The searches were limited to articles published in English from each database set-up date to 31 July 2009. Reference lists and relevant international conference proceedings were hand-searched. Fifty-five studies were identified, providing data on 5741 patients. The studies were classified into: (A) studies in which treatment of nausea/vomiting was the primary outcome (a total of 18 studies, of which eight studies specifically addressed opioid-induced emesis); and (B) studies in which nausea/vomiting were secondary or tertiary outcomes (37 studies). The existing evidence had several limitations, there was a lack of consistency and the overall quality was grade D. By applying the principles of the Grading of Recommendations Assessment, Development and Evaluations (GRADE) system, three weak recommendations were formulated. The current evidence is too limited to give evidence-based recommendations for the use of antiemetics for opioid-induced nausea or vomiting in cancer patients. The evidence suggests that nausea and vomiting in cancer patients receiving an opioid might be reduced by changing the opioid or opioid administration route. The evidence was also too limited to prioritize between symptomatic treatment and adjustment of the opioid treatment.
Development of a novel algorithm for human fall detection using wearable sensors

A novel algorithm for human fall detection by means of a tri-axial accelerometer, is described. A module constituted by the accelerometer and an on board processing unit was designed and realized. The system is conceived to be used in a multi-sensor network context for the remote monitoring of personnel working in very severe conditions (firefighters and civil protection operators). In the real application the module is thought to be integrated in the operator uniform collar. The algorithm is based on the detection of a critical trunk inclination in correspondence of an high rotational velocity. A Kalman filter was designed in order to separate the signal component due to gravity (i.e useful to extract the subject orientation) from the one due to the system acceleration. In comparison with the existing solutions the realized algorithm presents many advantages: no training is needed, low computational costs, fast time response and good performances also during critical activities (e.g jumping, running).
Monocyte chemotactic protein‐1, ‐2, and ‐3 are distinctively expressed in portal tracts and granulomata in primary biliary cirrhosis: implications for pathogenesis

The monocyte chemotactic proteins (MCPs) form a distinct structurally related subclass of C–C chemokines. MCPs select specific target cells due to binding to a distinct set of chemokine receptors and because of their effects on monocytes, and may participate in the process of granuloma formation during bacterial and/or mycobacterial infections. The aetiology of primary biliary cirrhosis (PBC) is still unclear, although bacterial infection and autoimmune processes have been implicated. In this study, the expression of three of the most potent monocyte chemoattractants, MCP‐1, ‐2, and ‐3, was examined in patients with PBC and the data were compared with results for other liver diseases including primary sclerosing cholangitis (PSC), chronic viral hepatitis C, hepatic sarcoidosis, and normal liver. MCP‐1 was expressed mainly in biliary epithelial cells of all liver specimens, irrespective of the cause of disease. Some mononuclear leukocytes in the portal tract expressed MCP‐1 in all the disease groups examined and there were no significant differences in frequency between these groups. In contrast, more than 80% of PBC livers showed MCP‐2‐ and MCP‐3‐positive mononuclear leukocyte infiltration in portal tracts, particularly around the bile ducts, whereas such cells were far less frequent in the other disease groups or in normal livers. Epithelioid granulomata of PBC patients contained MCP‐2‐ and MCP‐3‐positive cells at their edge. In double staining experiments, more than 60% of the MCP‐positive mononuclear cells co‐expressed CD68, suggesting that a proportion of MCP‐2‐ and MCP‐3‐positive cells are derived from monocytes. These monocytes expressing MCP‐2 and MCP‐3 may be responsible for the chemotactic activity of more monocytes. Such an expression pattern of MCP‐1, ‐2 and ‐3 in portal tracts seems to be distinctive for PBC. This pattern underlines the importance of MCP‐1, ‐2, and ‐3 in the recruitment of monocytes and possibly T lymphocytes into portal tracts, around the injured bile ducts, and into epithelioid granulomata in PBC. The data further implicate bacterial materials derived from bile in the overall pathogenesis of PBC. Copyright © 2000 John Wiley & Sons, Ltd.
Word retrieval in picture descriptions produced by individuals with Alzheimer’s disease

ABSTRACT What can tests of single-word production tell us about word retrieval in connected speech? We examined this question in 20 people with Alzheimer’s disease (AD) and in 20 cognitively intact individuals. All participants completed tasks of picture naming and semantic fluency and provided connected speech through picture descriptions. Picture descriptions were analyzed for total word output, percentages of content words, percentages of nouns, and percentages of pronouns out of all words, type–token ratio of all words and type–token ratio of nouns alone, mean frequency of all words and mean frequency of nouns alone, and mean word length. Individuals with AD performed worse than did cognitively intact individuals on the picture naming and semantic fluency tasks. They also produced a lower proportion of content words overall, a lower proportion of nouns, and a higher proportion of pronouns, as well as more frequent and shorter words on picture descriptions. Group differences in total word output and type–token ratios did not reach significance. Correlations between scores on tasks of single-word retrieval and measures of retrieval in picture descriptions emerged in the AD group but not in the control group. Scores on a picture naming task were associated with difficulties in word retrieval in connected speech in AD, while scores on a task of semantic verbal fluency were less useful in predicting measures of retrieval in context in this population.
Novel chemokine-like activities of histones in tumor metastasis

Histones are intracellular nucleosomal components and extracellular damage-associated molecular pattern molecules that modulate chromatin remodeling, as well as the immune response. However, their extracellular roles in cell migration and invasion remain undefined. Here, we demonstrate that histones are novel regulators of tumor metastasis with chemokine-like activities. Indeed, exogenous histones promote both hepatocellular carcinoma (HCC) cell migration and invasion through toll-like receptor (TLR)4, but not TLR2 or the receptor for advanced glycosylation end product. TLR4-mediated activation of nuclear factor-κB (NF-κB) by extracellular signal-regulated kinase (ERK) is required for histone-induced chemokine (e.g., C-C motif ligand 9/10) production. Pharmacological and genetic inhibition of TLR4-ERK-NF-κB signaling impairs histone-induced chemokine production and HCC cell migration. Additionally, TLR4 depletion (by using TLR4−/− mice and TLR4-shRNA) or inhibition of histone release/activity (by administration of heparin and H3 neutralizing antibody) attenuates lung metastasis of HCC cells injected via the tail vein of mice. Thus, histones promote tumor metastasis of HCC cells through the TLR4-NF-κB pathway and represent novel targets for treating patients with HCC.
Multiple-Subcarrier Modulation for Nondirected Wireless Infrared Communication

We examine multiple-subcarrier modulation (MSM) schemes for wireless infrared digital communication in the indoor environment. Intensity modulation with direct detection (IM/DD) is employed, which results in equivalent baseband channels with a nonnegativity constraint on the input. The power efficiencies of modulation schemes are compared at 30 Mb/s and 100 Mb/s over an ensemble of experimentally determined multipath channels. Carrier selection and power shaping are examined as methods for improving MSM performance. It is found that MSM schemes can allow operation at higher data rates than single-carrier modulation schemes without equalization. Moreover, MSM schemes can be more bandwidth-efficient and also can provide a simple and flexible method for multiple access to the channel. However, they are not as power efficient as single-carrier schemes, and this will limit their use to applications which are not power limited.
Implantation of an empty carbon fiber composite frame cage after single-level anterior cervical discectomy in the treatment of cervical disc herniation: preliminary results.

OBJECT The authors sought to evaluate retrospectively the radiological and clinical outcome of anterior cervical discectomy followed by implantation of an empty carbon fiber composite frame cage (CFCF) in the treatment of patients with cervical disc herniation and monoradiculopathy. METHODS Twenty-five consecutive patients (12 men, 13 women, mean age 45 years) with monoradiculopathy due to cervical disc herniation were treated by anterior cervical discectomy followed by implantation of an empty CFCF cage. On lateral flexion-extension radiographs segmental stability at a mean follow up of 14 months (range 5-31 months) was demonstrated in all 25 patients, and bone fusion was documented in 24 of 25 patients. The mean anterior intervertebral body height was 3.4 mm preoperatively and 3.8 mm at follow up in 20 patients. In these patients the mean segmental angle (angle between lower endplate of lower and upper vertebra) was 0.9 degrees preoperatively and 3.1 degrees at follow up. In the remaining five patients preoperative images were not retrievable. Self-scored neck pain based on a visual analog scale (1, minimum; 10, maximum) changed from a preoperative average of 5.6 to an average of 2 at follow up; radicular pain was reduced from 7.7 to 2.1 postoperatively. Analysis of the SF12 questionnaires showed a significant improvement in both the physical capacity score (preoperative mean 32.4 points; follow up 46 points) and the mental capacity score (preoperative mean 45.8 points; follow up 57.5 points). CONCLUSIONS Implantation of an empty CFCF cage in the treatment of cervical disc herniation and monoradiculopathy avoids donor site morbidity associated with autologous bone grafting as well as the use of any supplementary material inside the cage. Restoration or maintenance of intervertebral height and thus segmental lordosis and a very high rate of segmental stability and fusion are achieved using this technique.
Various Green Nanomaterials Used for Wastewater and Soil Treatment: A Mini-Review

The industrial intervention had a profound impact on our environment and the amount of damage it has bestowed is in the form of polluted water and solid waste accumulation. With upgradation of technologies in the industrial upfront there is also an increase in the robust nature of industrial waste. Keeping in view of the various environmental aspects, advanced techniques were developed by the inculcation of nanotechnology. Among the diverse technologies that have gained prominence is the use of nanoparticles as a medium to monitor and treatment process. One of them is the use of organic techniques, i.e., the use of nano-chitosan. The review article focuses on the new data pertaining to the study and improvement of various nano-scale treatment technologies implemented for wastewater treatment with an environment friendly biodegradation. The eradication of harmful toxicants is an exhaustive process, but this is required to invigorate the sustainable development. Various strategies were promulgated but the inculcation of nanomaterial provided a low-cost, efficient and simple method for removal of contaminants by adsorption. These nanomaterials provide the best adsorbent due to its simple structural properties that provide a better surface area with high absorption capacity. This comprehensive review gives a glimpse of the various green and other nanotechnologies used for wastewater and contaminated soil treatments.
Moderate to vigorous physical activity interactions with genetic variants and body mass index in a large US ethnically diverse cohort

Genome‐Wide Association Studies have successfully identified numerous genetic loci that influence body mass index in European‐descent middle‐aged adults. Adolescence is a high‐risk period for the development of adult obesity and severe obesity. Physical activity is one of the most promising behavioural candidates for preventing and reducing weight gain, particularly among youth.
Hydrophilic nanofibers as new supports for thin film composite membranes for engineered osmosis.

Engineered osmosis (e.g., forward osmosis, pressure-retarded osmosis, direct osmosis) has emerged as a new platform for applications to water production, sustainable energy, and resource recovery. The lack of an adequately designed membrane has been the major challenge that hinders engineered osmosis (EO) development. In this study, nanotechnology has been integrated with membrane science to build a next generation membrane for engineered osmosis. Specifically, hydrophilic nanofiber, fabricated from different blends of polyacrylonitrile and cellulose acetate via electrospinning, was found to be an effective support for EO thin film composite membranes due to its intrinsically wetted open pore structure with superior interconnectivity. The resulting composite membrane exhibits excellent permselectivity while also showing a reduced resistance to mass transfer that commonly impacts EO processes due to its thin, highly porous nanofiber support layer. Our best membrane exhibited a two to three times enhanced water flux and 90% reduction in salt passage when compared to a standard commercial FO membrane. Furthermore, our membrane exhibited one of the lowest structural parameters reported in the open literature. These results indicate that hydrophilic nanofiber supported thin film composite membranes have the potential to be a next generation membrane for engineered osmosis.
The Xmrk receptor tyrosine kinase is activated in Xiphophorus malignant melanoma.

Xmrk encodes a putative transmembrane glycoprotein of the tyrosine kinase family and is a melanoma‐inducing gene in Xiphophorus. We attempted to investigate the biological function of the putative Xmrk receptor by characterizing its signalling properties. Since a potential ligand for Xmrk has not yet been identified, it has been difficult to analyse the biochemical properties and biological function of this cell surface protein. In an approach towards such analyses, the Xmrk extracellular domain was replaced by the closely related ligand‐binding domain sequences of the human epidermal growth factor receptor (HER) and the ligand‐induced activity of the chimeric HER‐Xmrk protein was examined. We show that the Xmrk protein is a functional receptor tyrosine kinase, is highly active in malignant melanoma and displays a constitutive autophosphorylation activity possibly due to an activating mutation in its extracellular or transmembrane domain. In the focus formation assay the HER‐Xmrk chimera is a potent transforming protein equivalent to other tyrosine kinase oncoproteins.
Direct selection of human bone marrow mesenchymal stem cells using an anti‐CD49a antibody reveals their CD45med,low phenotype

Summary. Human bone marrow mesenchymal stem cells (MSC) generate, via a fibroblast colony‐forming unit (CFU‐F), osteo‐chondroblastic cells as well as adipocytes and stromacytes. To date, these stem cells are isolated indirectly using a cell culture method and phenotyped as CD45 negative while the in vivo counterparts are undetermined. Our aim was to develop a direct selection method and to determine the phenotype of the MSC isolated in this way. Mesenchymal cells were selected with anti‐CD49a and/or anti‐CD45 antibodies using either flow cytometry or a magnetic beads method. All CFU‐F were always detected in the small population of CD49a‐positive cells. These CFU retained their differentiation potential and gave rise to osteo‐chondroblastic cells, adipocytes and stromacytes. Phenotypic studies on uncultured cells revealed a CD45med,low, CD34low, HLA‐II– cell population. Flow cytometry cell sorting showed that MSC with CFU‐F potential were obtained only from a CD49a+/CD45med,low population. In addition, when cultured, they clearly became CD45–, CD34–, HLA‐II–, CD49a+. These results confirmed that MSC can be directly selected easily from human bone marrow using magnetic beads without altering their differentiation potential. These cells expressed mildly the haematopoietic marker CD45, which was dramatically downregulated by in vitro culture. The expression of CD45 coupled to CD49a thus enabled direct selection of the MSC.
De la infografía a la visualización de datos: Impacto del Big Data en el diseño de la información

Presentamos un trabajo sobre los procesos que se producen en torno a la visualización de la información. A partir de las técnicas de visualización gráfica utilizadas desde la prehistoria surge el concepto de infografía en el ámbito periodístico con la digitalización de los procesos en los años 90. La incorporación del Big Data otorga nuevas funciones a la visualización de datos y nos obliga entonces a replantearnos si ambos términos hacen referencia a las mismas prácticas de representación gráfica, o bien son procesos técnicos independientes con entidad propia, y en tal caso, determinar lo que corresponde a cada uno.