Miracle-dz/UniFAR-FTUs huggingface dataset:
Impact of Environmental Microbes on the Composition of the Gut Microbiota of Adult BALB/c Mice

To investigate the impact of microbes within the living environment on the gut microbiota of adults, we raised three groups of BALB/c mice from 3–4 weeks age in the same specific-pathogen-free animal room for 8 weeks. The control group lived in cages with sterilized bedding (pelletized cardboard), the probiotics group had three probiotics added to the sterilized bedding, and the intestinal microbes (IM) group had the intestinal microbes of a healthy goat added to the bedding. All other variables such as diet, age, genetic background, physiological status, original gut microbiota, and living room were controlled. Using high-throughput sequencing of the 16S rRNA gene, we observed that the control and probiotics groups had similar diversity and richness of gut microbiota. The two groups had significantly lower diversity than the IM group. We also observed that the IM group had a specific structure of gut microbial community compared with the control and probiotics groups. However, the dominate bacteria changed slightly upon exposure to intestinal microbes, and the abundance of the non-dominate species changed significantly. In addition, exposure to intestinal microbes inhibited DNFB-induced elevation of serum IgE levels. Our results provide new evidence in support of the microflora and hygiene hypotheses.
Understanding Transition Experiences of Combat Veterans Attending Community College

ABSTRACT The majority of research concerning student veterans has been conducted at the university level, with minimum analysis performed at the level where the vast majority of returning veterans attend school: the community college. While some research has discussed what services colleges and universities should offer returning veterans, little research has been conducted on understanding the actual experiences of veterans making the transition from service member to college student. A group of varied gender and racial backgrounds took part in an effort to describe the lived experiences of combat veterans making the transition into community college after active military service. Findings include the inadequacy of current models for use in understanding student-veteran transition experiences, particularly at the community college, and the discovery that the majority of student veterans involved in this study do not take part in on-campus programs specifically designed for them. The experiences of military veterans who enroll in community colleges subsequent to deployment in a combat environment since 11 September 2001 have not been adequately researched and remain misunderstood (Ewing, 2011; Gomez, 2011; Karni, 2011; Wood, 2011). Existing models of student transition used to describe the student-veteran experience are largely inadequate and framed around traditional 4-year colleges and universities. As a result, higher education stakeholders may not have the necessary information to effectively assist this growing student demographic. Further research will increase the body of knowledge in this important area and, it is hoped, lead to more effective educational policies regarding student veterans.
A structured light laser probe for gastrointestinal polyp size measurement: a preliminary comparative study

Abstract Background and study aims Polyp size measurement is an important diagnostic step during gastrointestinal endoscopy, and is mainly performed by visual inspection. However, lack of depth perception and objective reference points are acknowledged factors contributing to measurement errors in polyp size. In this paper, we describe the proof-of-concept of a polyp measurement device based on structured light technology for future endoscopes. Patients and methods Measurement accuracy, time, user confidence, and satisfaction were evaluated for polyp size assessment by (a) visual inspection, (b) open biopsy forceps of known size, (c) ruled snare, and (d) structured light probe, for a total of 392 independent polyp measurements in ex vivo porcine stomachs. Results Visual assessment resulted in a median estimation error of 2.2 mm, IQR = 2.6 mm. The proposed probe can reduce the error to 1.5 mm, IQR = 1.67 mm (P = 0.002, 95 %CI) and its performance was found to be statistically similar to using forceps for reference (P = 0.81, 95 %CI) or ruled snare (P = 0.99, 95 %CI), while not occluding the tool channel. Timing performance with the probe was measured to be on average 54.75 seconds per polyp. This was significantly slower than visual assessment (20.7 seconds per polyp, P = 0.005, 95 %CI) but not significantly different from using a snare (68.5 seconds per polyp, P = 0.73, 95 %CI). However, the probe’s timing performance was partly due to lens cleaning problems in our preliminary design. Reported average satisfaction on a 0 – 10 range was highest for the proposed probe (7.92), visual assessment (7.01), and reference forceps (7.82), while significantly lower for snare users with a score of 4.42 (P = 0.035, 95 %CI). Conclusions The common practice of visual assessment of polyp size was found to be significantly less accurate than tool-based assessment, but easy to carry out. The proposed technology offers an accuracy on par with using a reference tool or ruled snare with the same satisfaction levels of visual assessment and without occluding the tool channel. Further study will improve the design to reduce the operating time by integrating the probe within the scope tip.
Cholesterol negatively regulates IL-9–producing CD8+ T cell differentiation and antitumor activity

CD8+ T cells can be polarized into IL-9–secreting (Tc9) cells. We previously showed that adoptive therapy using tumor-specific Tc9 cells generated stronger antitumor responses in mouse melanoma than classical Tc1 cells. To understand why Tc9 cells exert stronger antitumor responses, we used gene profiling to compare Tc9 and Tc1 cells. Tc9 cells expressed different levels of cholesterol synthesis and efflux genes and possessed significantly lower cholesterol content than Tc1 cells. Unique to Tc9, but not other CD8+ or CD4+ T cell subsets, manipulating cholesterol content in polarizing Tc9 cells significantly affected IL-9 expression and Tc9 differentiation and antitumor response in vivo. Mechanistic studies showed that IL-9 was indispensable for Tc9 cell persistence and antitumor effects, and cholesterol or its derivatives inhibited IL-9 expression by activating liver X receptors (LXRs), leading to LXR Sumoylation and reduced p65 binding to Il9 promoter. Our study identifies cholesterol as a critical regulator of Tc9 cell differentiation and function.
Vibratory courtship in a web-building spider: signalling quality or stimulating the female?

Courtship behaviour in spiders in the form of premating vibrations by males may function (1) as a male identity signal used for species recognition, (2) in suppression of female aggressiveness, (3) to stimulate female mating behaviour, or (4) as a quality signal used in female choice. We investigated the function of web vibration by male Stegodyphus lineatus in a series of experiments. Regardless of vibratory performance, all males mated successfully with virgin females but only 56.4% of males mated with nonvirgin females. Vibratory performance did not influence male mating success, but heavier males had a higher probability of mating with mated females. Males vibrated less often and produced fewer vibrations when introduced on the web of a mated female. Males that vibrated webs of virgin females mated faster than nonvibrating males, but there was no effect of vibration rate or body mass. There was no effect of male vibratory effort or vibration rate on female reproductive success measured as time to egg laying, clutch size, number of hatched young, number of dispersed young and offspring body mass after a single mating. Males vibrated on abandoned virgin female webs but the response decreased with increasing duration of female absence, suggesting that females produce a web-borne pheromone, which elicits male vibrating behaviour. Mated females were less receptive and not stimulated by male vibrating behaviour. We conclude that male premating vibrations in S. lineatus do not function as a male quality signal selected via female choice. Rather, the primary function of this behaviour may be to stimulate a receptive female to mate.
Partial participation towards collective action: To stifle or instigate

In this paper we extend the Granovetter threshold model with partial participation towards a collective action. That is, agents may partake by conducting an action that is less costly than the ultimate collective action, but costly enough to signal a commitment to the cause. We show that it is not just the exact distribution of thresholds, but also the distribution of available actions that determines whether a collective action will be achieved. We suggest and prove propositions for how both an inventive “activist” and a “dictator” may strategically change the signaling value of existing actions, or introduce new ones, in order to either instigate or stifle collective action. Applying the theory to revolutions, we argue that new technology can play a role beyond that of communication and synchronization, viz. that of adding modes of partial, less arduous, participation.
A multisensor detector of a sleep apnea for using at home

Diagnosis of obstructive sleep apnea usually involves polysomnographic analysis, which unfortunately requires overnight stay in a specialized clinic and is very uncomfortable for a patient. This paper describes the method and apparatus for recording a set of signals to detect sleep apnea. The device records the following signals simultaneously: three-channel ECG, respiratory functions, signals from the accelerometer, and snoring sounds. Measurements are carried out during the night, in the patient's home. Algorithm for automatic analysis of the data can be used to detect sleep apnea and hypopnea.
Antibiotic Sensitivity Profiling and Virulence Potential of Campylobacter jejuni Isolates from Estuarine Water in the Eastern Cape Province, South Africa

Campylobacter jejuni (CJ) is a zoonotic microbe and a major causative organism of diarrheal infection in humans that often has its functional characteristics inactivated in stressed conditions. The current study assessed the correlation between recovered CJ and water quality parameters and the drug sensitivity patterns of the pathogen to frontline antibiotics in human and veterinary medicine. Water samples (n = 244) from rivers/estuarines were collected from April–September 2016, and physicochemical conditions were recorded on-site. CJ was isolated from the samples using standard microbiological methods and subjected to sensitivity testing to 10 antibiotics. Mean CJ counts were between 1 and 5 logs (CFU/mL). Ninety-five isolates confirmed as CJ by PCR showed varying rates of resistance. Sensitivity testing showed resistance to tetracycline (100%), azithromycin (92%), clindamycin (84.2%), clarithromycin and doxycycline (80%), ciprofloxacin (77.8%), vancomycin (70.5%), erythromycin (70%), metronidazole (36.8%) and nalidixic acid (30.5%). Virulence encoding genes were detected in the majority 80/95, 84.2%) of the confirmed isolates from cdtB; 60/95 (63.2%) from cstII; 49/95 (51.6%) from cadF; 45/95 (47.4%) from clpP; 30/95 (31.6%) from htrB, and 0/95 (0%) from csrA. A multiple resistance cmeABC active efflux pump system was present in 69/95 (72.6) isolates. The presence of CJ was positively correlated with temperature (r = 0.17), pH (r = 0.02), dissolved oxygen (r = 0.31), and turbidity (r = 0.23) but negatively correlated with salinity (r = −0.39) and conductivity (r = −0.28). The detection of multidrug resistant CJ strains from estuarine water and the differential gene expressions they possess indicates a potential hazard to humans. Moreover, the negative correlation between the presence of the pathogen and physicochemical parameters such as salinity indicates possible complementary expression of stress tolerance response mechanisms by wild-type CJ strains.
Pulse-induced resistive and capacitive switching in TiO2 thin film devices

In this study, we exploit the non-zero crossing current–voltage characteristics exhibited by nanoscale TiO2 based solid-state memristors. We demonstrate that the effective resistance and capacitance of such two terminal devices can be modulated simultaneously by appropriate voltage pulsing. Our results prove that both resistive and capacitive switching arise naturally in nanoscale Pt/TiO2/Pt devices under an external bias, this behaviour being governed by the formation/disruption of conductive filaments through the TiO2 thin film.
Face Liveness Detection Based on Perceptual Image Quality Assessment Features with Multi-scale Analysis

Vulnerability of recognition systems to spoofing attacks (presentation attacks) is still an open security issue in the biometrics domain. Among all biometric traits, face is exposed to the most serious threat since it is particularly easy to access and reproduce. In this paper, an effective approach against face spoofing attacks based on perceptual image quality assessment features with multiscale analysis is presented. First, we demonstrate that the recently proposed blind image quality evaluator (BIQE) is effective in detecting spoofing attacks. Next, we combine the BIQE with an image quality assessment model called effective pixel similarity deviation (EPSD), which we propose to obtain the standard deviation of the gradient magnitude similarity map by selecting effective pixels in the image. A total number of 21 features acquired from the BIQE and EPSD constitute the multi-scale descriptor for classification. Extensive experiments based on both intradataset and cross-dataset protocols were performed using three existing benchmarks, namely, Replay-Attack, CASIA, and UVAD. The proposed algorithm demonstrated its superiority in detecting face spoofing attacks over many state of the art methods. We believe that the incorporation of the image quality assessment knowledge into face liveness detection is promising to improve the overall accuracy.
Efficient excitation and collection of single-molecule fluorescence close to a dielectric microsphere

Dielectric microspheres illuminated by a tightly focused Gaussian beam can focus light on a tiny spot with subwavelength dimensions along the three directions of space. We report here a detailed experimental and theoretical study of the interaction between a single fluorescent molecule and this peculiar electromagnetic distribution. The microsphere increases the excitation intensity sensed by the molecule up to a factor of 2.2, while at the same time it allows for a collection efficiency of up to 60% by redirecting the light emitted at large incidences toward the optical axis. By combining these two effects, the number of collected fluorescence photons can be increased up to a factor of 5. We quantify the evolution of the excitation and collection contributions with the microsphere dimensions and compare our experimental findings with numerical simulations.
The role of neonatal pulmonary morbidity in the longitudinal patterns of hospitalisation for respiratory infection during the first year of life

Abstract Respiratory infections among infants constitute a major burden to health care systems in developed nations, yet the course and risk factors leading to these conditions are poorly understood. We examine the longitudinal patterns of respiratory infection hospitalisation (RIH) and how these patterns are influenced by neonatal pulmonary morbidities. We included all live births (n = 429 058) occurring in the Australian state of Queensland between January 2009 and December 2015. Data were structured so that each participant had a record (present/absent) of RIH for each month from birth to 12 months. Initially, latent class growth analysis was used to identify the trajectories of RIH adjusted for spatial–temporal factors; using the identified trajectories of RIH as outcomes, we built a multinomial logistic regression model to identify neonatal predictors of RIH trajectories. Our results indicated that a four-class solution was the best fit to the data, comprising a ‘no-risk’ trajectory, a ‘low-risk’ trajectory, an ‘early-risk’ trajectory and a ‘chronic-risk’ trajectory. Compared with the no-risk trajectory, membership in the other trajectories was predicted by a range of neonatal pulmonary morbidities, with transient tachypnoea of newborn showing a specific relationship with the early-risk group and sleep apnoea showing a specific and strong risk with the chronic-risk group. Our findings suggest the possibility of identifying neonates at risk of recurrent RIH and implementing effective intervention strategies prior to neonatal discharge.
Niching Pareto Ant Colony Optimization Algorithm for Bi-Objective Pathfinding Problem

In this paper, we propose a niching Pareto ant colony optimization (NPACO) algorithm to solve the bi-objective pathfinding problem. First, based on a planar navigable data model, three different searching area restricted methods are proposed and compared. In addition, a node simplification strategy is introduced to simplify nodes that exist in network branch loops, eliminating the redundant search time in the branch loops. Afterward, we propose the elitist ants and weakened strategy for an ACO to overcome the problem caused by the impact of accumulated pheromone on the suboptimal path and apply the strategy to a PACO for urban city pathfinding. Finally, the niching method is adopted to simultaneously locate and maintain multiple optimal solutions to increase search robustness. The experimental results show that the NPACO with a restricted and simplified search area returns a Pareto optimal solution set that is uniformly distributed along the Pareto frontier with low computational complexity.
In-Depth Analysis and Characteristics of the Traveling Thief Problem

This thesis presents an in-depth analysis and characteristics of the quite new and complex Traveling Thief Problem, where the well-known Traveling Salesman Problem and Knapsack Problem interact. Since the Traveling Thief Problem is an interwoven system, the interaction between the components is explained and visualized in detail. The single as well as the multi-objective formulation and the different models of the problem are defined and an example scenario is presented in order to show how the objective values, time and profit, are calculated. Furthermore, we propose another model, which uses an extra constraint instead of a weight vector for composing the two objectives, and is therefore embeddable in a multi-objective -constraint algorithm. To encourage doing research on the multi-objective problem, characteristics of the two-dimensional objective space are presented and the usefulness of algorithms for the interacting subproblems is investigated. Moreover, three problem solving strategies for the single-objective problem are proposed and existing algorithms are classified into these categories. Also, some current research questions using experimental studies on small-scale instances are answered and different solutions of a case study found by state-of-the-art algorithms are visualized. All analyses focus on the interdependence and interwovenness of the components. The obtained results provide insights into characteristics of the single and multi-objective Traveling Thief Problem and new starting points for ongoing research.
Mortality in a cohort of asbestos-exposed workers undergoing health surveillance

Background: The coastal area of Friuli Venezia Giulia (FVG) region, north-eastern Italy, was characterized by work activities in which asbestos was used until the early 1990s, particularly in shipbuilding. A public health surveillance program (PHSP) for asbestos-exposed workers was established, although limited evidence exists about the efficacy of such programs in reducing disease occurrence and mortality. Objectives: To compare mortality in a cohort of 2,488 men occupationally exposed to asbestos, enrolled in a PHSP in FVG between the early 1990s and 2008, with that of the general population of FVG and Italy. Methods: Standardized Mortality Ratios (SMR), with 95% Confidence Interval (95% CI), for all causes, all cancers, lung (LC) and pleural cancer (PC) were estimated in the cohort and in subgroups of workers with the first hire in shipbuilding that caused asbestos exposure (<1974, 1974-1984, 1985-1994). Results: A strong excess in mortality for PC with reference to FVG (SMR=6.87, 95% CI 4.45-10.17) and Italian population (SMR=13.95, 95% CI 9.02-20.64) was observed. For LC, the FVG-based SMR was 1.49 (95% CI 1.17-1.89) and the Italy-based 1.43 (95% CI 1.12-1.81). Mortality among workers with the first hire in shipbuilding before 1974 was high for PC (FVG-based SMR=8.98, 95% CI 5.56-13.75; Italy-based SMR=18.41, 95% CI 11.40-28.17) and for LC (FVG-based SMR =1.60, 95% CI 1.18-2.11; Italy-based SMR=1.54, 95% CI 1.14-2.03). Further, for LC between 1974 and 1984, the FVG-based SMR was 2.45 (95% CI 1.06-4.82), and the Italy-based SMR was 2.33 (95% CI 1.01-4.60). Conclusions: This cohort experienced an excess mortality for pleural and lung cancer, compared with regional and national populations. For lung cancer, the excess was stronger in workers with the first hire in shipbuilding before 1985, suggesting a key role of asbestos exposure.
Bioactive pedicle screws prepared by chemical and heat treatments improved biocompatibility and bone-bonding ability in canine lumbar spines

Background Titanium (Ti)-6Al-4V alloy, which is widely used in spinal instrumentation with a pedicle screw (PS) system. However, significant clinical problems, including loosening and back-out of PSs, persist. During the last decade, a novel technology that produces bioactive Ti from chemical and heat treatments has been reported that induces the spontaneous formation of a hydroxyapatite (HA) layer on the surface of Ti materials. The purpose of this study was to study the effect of bioactivation of Ti-6Al-4V PSs on the ability of HA formation in vitro and its biocompatibility and bone-bonding ability in vivo. Methods Ti-6V-4Al alloy PSs were prepared and bioactivated by NaOH-CaCl2-heat-water treatments. The HA-forming ability of bioactive PSs in simulated body fluid (SBF) was evaluated by field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray analysis (EDX). Six 11-month-old female beagle dogs were used for the in vivo study. Bioactive and control (without bioactivation) PSs were left and right randomly placed from L1 to L6. One and three months after surgery, lumbar spines were removed for biomechanical and histological analyses. Results In vitro: The surface analysis of bioactive PSs by FE-SEM and EDX showed substantial HA deposits over the entire surface. In vivo: The mean extraction torque was significantly higher for bioactive PSs compared to controls PSs (P<0.01); there was no significant difference in pull-out strength between control and bioactive PSs. Histologically, the contact area between bone tissue and screw surface showed no significant trend to be greater in bioactive PSs compared to control PSs (P = 0.06). Conclusions Bioactive PSs prepared by chemical and heat treatments formed layers of HA on the surface of screws in vitro that improved biocompatibility and bonding ability with bone in vivo. Bioactive PSs may reduce screw loosening to overcome the obstacles confronted in spinal instrumentation surgery.