Miracle-dz/UniFAR-FTUs huggingface dataset:
ECONOMIC VALUE OF ECOSYSTEM SERVICES IN THE EASTERN ORE MOUNTAINS

The paper presents the results of a research project aiming at determining the economic value of selected ecosystem services in the Eastern Ore Mountains. The use of the dynamically evolving concept of economic services, used to identify economically utilisable services provided by undamaged ecosystems, can thus contribute to dealing with the issues of optimum landscape management. The primary data collection took place in the summer of 2013; a total of 216 questionnaires were collected. The research focused on three ecosystems, namely montane meadows, clearance cairns and quasi-natural mountain streams. The paper presents and discusses primarily the results of a choice experiment that was used to determine the willingness to pay for de ﬁ ned changes in the ecosystems examined. The research showed that visitors to the Eastern Ore Mountains unambiguously prefer a natural (authentic) or quasi-natural condition of the landscape and that people are able to distinguish among the different situations well (e.g., different appearance of montane meadows, mountain streams, clearance cairns, etc.). People prefer montane meadows scythed without farm animals, clearance cairns not overgrown, and streams with a quasi-natural character. The authors point out the ﬁ nding that the research results indicate a strong connection of the local population to the local landscape and ecosystems, the potential of which in relation to nature protection and landscape management is totally unutilised at the moment. Special attention is paid to a speci ﬁ c ecosystem, not studied yet in the Czech Republic – the clearance cairns, which showed (contrary to expectations) a noticeably higher willingness to pay for their good management compared to the other ecosystems examined.
Education Policy Influence Efficacy: Teacher Beliefs in Their Ability to Change Education Policy.

Guided by Social Cognitive Theory (SCT) this study sought to understand how teachers view themselves as educational policy actors and to construct an instrument that measures teacher beliefs about their aptitude to advocate for changes in educational policy. This study employed a sequential explanatory design, characterized by quantitative data collection from approximately 250 teachers followed by qualitative interviews with seven teachers. Findings reveal that teachers are not overly confident in their ability to make change and see themselves as implementers more than creators of education policy.
Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking

ADP-ribosylation factor (ARF)-related protein 1 (ARFRP1) is a small GTPase with significant similarity to the ARF family. However, little is known about the function of ARFRP1 in mammalian cells, although knockout mice of its gene are embryonic lethal. In the present study, we demonstrate that ARFRP1 is associated mainly with the trans-Golgi compartment and the trans-Golgi network (TGN) and is an essential regulatory factor for targeting of Arl1 and GRIP domain-containing proteins, golgin-97 and golgin-245, onto Golgi membranes. Furthermore, we show that, in concert with Arl1 and GRIP proteins, ARFRP1 is implicated in the Golgi-to-plasma membrane transport of the vesicular stomatitis virus G protein as well as in the retrograde transport of TGN38 and Shiga toxin from endosomes to the TGN.
Access to health care in a system transition: the case of Bulgaria.

OBJECTIVE Throughout the 1990s, the Soviet-style model in central and eastern Europe that provided free health services has been subject to radical reforms. Socio-economic inequalities have also increased but there is little information on inequalities in health care utilization. This paper examines the pattern of illness behaviour in Bulgaria, seeking evidence of inequalities in access to services and eliciting users' pathways to care. DESIGN Analysis drew on a representative population survey in Bulgaria (1997). The financial determinants of service use were tested in a multivariate model adjusted first for age, and then for age, marital status and self-reported health. In-depth interviews with users and providers addressed pathways to care, use of connections and other informal strategies to obtain care. RESULTS As expected, rates of illness vary with income, with highest rates among the poor. After adjustment for illness, consultation rates are relatively equal across income levels, with the exception of worse-off women who tend to consult more. For first contact, there are few differences according to income, with the better off preferring secondary level. Pathways slightly differ, with women more often treated in primary care. Private sector utilization is low. Qualitative research reveals well-established strategies to obtain more advanced care, including use of connections, informal payments and use of emergency services. CONCLUSIONS An apparent lack of inequalities in access to care conceals a more complex picture in which income and gender influence the pathways taken through the system.
Augmented Delayed Infarct Expansion and Reactive Astrocytosis after Permanent Focal Ischemia in Apolipoprotein E4 Knock-In Mice

Using homozygous human apolipoprotein E2 (apoE2) (2/2)-, apoE3 (3/3)-, or apoE4 (4/4)-knock-in (KI) mice, we aimed to examine whether an apoE isoform-specific exacerbation of delayed infarct expansion occurs after permanent middle cerebral artery occlusion (pMCAO). Compared with 2/2- or 3/3-KI mice, 4/4-KI mice exhibited significantly larger infarct volumes and worse neurologic deficits after pMCAO, with no significant differences between the latter two groups. Infarct volume in 4/4-KI mice was significantly increased from 1 to 5 days after pMCAO, whereas that in 2/2- or 3/3-KI mice was not significantly altered. DNA fragmentation in the peri-infarct area as detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphatenick end-labeling was increased to a similar degree in all of the KI mice by 5 days after pMCAO, with no significant differences among the mouse groups. At every time-point examined, human apoE was most markedly expressed in the peri-infarct area, with similar immunoreactivity among the three lines of KI mice. The glial fibrillary acidic protein immunoreactive burden in the peri-infarct area was progressively increased through 7 days in 4/4-KI mice, but not in 2/2- or 3/3-KI mice. Taken together, these data show that the apoE4 isoform acts to aggravate delayed infarct expansion and peri-infarct reactive astrocytosis during the subacute phase of pMCAO in genetically engineered apoE-KI mice.
Efficient generation of transgenic mice by lentivirus‐mediated modification of spermatozoa

Transgenic technologies conventionally rely on the oocyte as a substrate for genetic modification. Owing to their accessibility, however, male germ cells, including mature sperm, have material advantages for use in transgenesis. Here we have exploited lentiviruses to generate transgenic animals via the male germline. When pseudotyped lentiviral vectors encoding green fluorescent protein (GFP) were incubated with mouse spermatozoa, these sperm were highly successful in producing transgenics. Lentivirally transduced mouse spermatozoa were used in in vitro fertilization (IVF) studies, and when followed by embryo transfer, ≥42% of founders were found to be transgenic for GFP. Inverse PCR strategy for integration site analysis demonstrated integration of at least 1 or 2 copies of GFP in the transgenics, mapping to different chromosomes. GFP expression was detected in a wide range of murine tissues, including testis and the transgene was stably transmitted to a third generation of transgenic animals. This relatively simple, yet highly efficient, technique for generating transgenic animals by transducing spermatozoa with lentiviral vectors in vitro is a powerful tool for the study of fertilization/preimplantation development, vertical viral gene transmission, gene function and regulation, and epigenetic inheritance. —Chandrashekran, A. , Sarkar, R. , Thrasher, A. , Fraser, S. E. , Dibb, N. , Casimir, C. , Readhead, C. , Winston, R. Efficient generation of transgenic mice by lentivirus‐mediated modification of spermatozoa. FASEB J. 28, 569–576 (2014). www.fasebj. org
Similar Synapse Density in Layer IV Columns of the Primary Somatosensory Cortex of Transgenic Mice with Different Brain Size: Implications for Mechanisms Underlying the Differential Allocation of Cortical Space

The relative dimension of the areas constituting the cerebral cortex differs greatly in the brains of different mammalian species. The mechanisms by which such an evolutionary remodeling has occurred is not well understood. To begin exploring possible mechanisms, we took advantage of a transgenic mouse model in which the area of the primary somatosensory cortex (S1) shifts, to some extent independent from the area of the cortex as a whole, as a result of differences in the availability of insulin-like growth factor I (IGF-I). Electron microscopy estimations of synapse density in D3 and C3 cortical columns of the S1 layer IV revealed that this parameter was similar among wild type and transgenic mice with higher and lower availability of IGF-I. Because D3 and C3 columns were larger and smaller than normal in mice with higher and lower IGF-I availability, the total number of synapses contained in the average area of D3 and C3 columns increased and decreased, respectively. No differences in the number and overall arrangement of S1 columns were observed among animal groups. These results suggest that: 1) synapse density is a constant factor within the S1 cortical column structure; 2) the mechanisms and factors regulating cell number and synaptogenesis are affected as columns and cortical areas modify their relative dimensions; 3) altered availability of neurotrophic factors might be associated with changes in areal dimensions; and 4) changes in cortical areal dimensions within single lineages might result from the addition of minicolumns to preexisting columns.
Comparison of the Physiological Responses to Different Small-Sided Games in Elite Young Soccer Players

Köklü, Y, Aşçi, A, Koçak, FÜ, Alemdaroğlu, U, and Dündar, U. Comparison of the physiological responses to different small-sided games in elite young soccer players. J Strength Cond Res 25(6): 1522-1528, 2011—The purpose of this study was to compare the blood lactate (La−), heart rate (HR) and percentage of maximum HR (%HRmax) responses among the small-sided games (SSGs) in elite young soccer players. Sixteen players (average age 15.7 ± 0.4 years; height 176.8 ± 4.6 cm; body mass 65.5 ± 5.6 kg; &OV0312;o2max 53.1 ± 5.9 ml·kg−1·min−1; HRmax 195.9 ± 7.4 b·min−1) volunteered to perform the YoYo intermittent recovery test and 6 bouts of soccer drills including 1-a-side, 2-a-side, 3-a-side, and 4-a-side games without a goalkeeper in random order at 2-day intervals. The differences in La−, HR and %HRmax either among the SSGs or among the bouts were identified using 4 × 6 (games × exercise bouts) 2-way analysis of variance with repeated measures. Significant differences were found on La−, HR, and %HRmax among the bouts (p ≤ 0.05). The 3-a-side and 4-a-side games were significantly higher than 1-a-side and 2-a-side games on HR and %HRmax (p ≤ 0.05), whereas the 1-a-side game significantly resulted in higher La− responses compared to other SSGs. This study demonstrated that physiological responses during the 1-a-side and 2-a-side games were different compared to 3-a-side and 4-a-side games. Therefore, it can be concluded that a decreased number of players results in increased intensity during SSGs including 6 bouts. These results suggest that coaches should pay attention on choosing the SSG type and the number of bouts to improve desired physical conditioning of elite young soccer players in soccer training.
Thrombosis in Continuous‐Flow Left Ventricular Assist Devices: Pathophysiology, Prevention, and Pharmacologic Management

Continuous‐flow left ventricular assist devices reduce short‐term mortality and improve quality of life in patients with end‐stage heart failure. Unfortunately, device‐related complications remain common, with many patients experiencing adverse events within the first year. New literature suggests that rates of device‐related thrombosis may be increasing since 2011, which is particularly troublesome given that this pathology can result in a disabling stroke, organ damage, and death. In 2013, a group of practitioners in the field of mechanical circulatory support published a treatment algorithm based on their expert opinion. However, a comprehensive review of the pharmacotherapy of this condition is lacking. A search of the literature revealed 20 separate publications of case reports or case series describing outcomes associated with the use of drug therapy for suspected pump thrombosis. Each of these experiences was limited by small sample size, nonrandomized treatment allocation, and nonstandardized medication dosing. Data describing the outcomes of surgical versus medical management of device thrombosis are also sparse, with only three published reports identified. Based on the review of this limited literature, surgical management appears to be the preferred treatment modality, especially in those with organ hypoperfusion or hemodynamic instability. In patients ineligible for surgery, pharmacotherapy options remain limited. Use of all drug classes described in the literature for the HeartMate II device—fibrinolytics, glycoprotein IIb/IIIa inhibitors, and direct thrombin inhibitors—was hindered by either marginal efficacy or bleeding. Based on historical experience with unfractionated heparin in patients under HeartMate II support, we recommend this agent as a possible option for those with suspected pump thrombosis in lieu of surgical device exchange. For the HeartWare HVAD, limited data suggest that direct intraventricular administration of alteplase may be an acceptable treatment alternative. Additional research is clearly needed to further delineate the role of pharmacotherapy and to identify the optimal agent for managing this potentially life‐threatening condition.
Acne patients frequently associated with abnormal plasma lipid profile

This study was designed to investigate the relationship between plasma lipid profile and acne. Acne patients (n = 181) and healthy volunteers (n = 130) matched in terms of both age and sex were enrolled. Plasma total cholesterol (TC), triglyceride (TG), high‐density lipoprotein cholesterol, low‐density lipoprotein cholesterol (LDL‐C) and lipoprotein (LP)(a) levels were measured. TC, LDL‐C and LP(a) levels in male and female patients with severe acne were significantly higher than in the healthy control group (P < 0.05). TG in male patients with severe and moderate acne was significantly higher than in the healthy control group (P < 0.05). LP(a) in male and female patients with mild, moderate and severe acne was significantly higher than in the healthy control group (P < 0.05). The constituent ratio of male and female patients with TC, TG, LDL‐C and LP(a) over the normal range was signi‐ficantly higher than in the healthy control group. In this study, acne patients were frequently associated with abnormal lipid profile, providing a new basis for further exploration of the pathogenesis, as well as new treatments, of acne vulgaris.
Wnk1 kinase deficiency lowers blood pressure in mice: A gene-trap screen to identify potential targets for therapeutic intervention

The availability of both the mouse and human genome sequences allows for the systematic discovery of human gene function through the use of the mouse as a model system. To accelerate the genetic determination of gene function, we have developed a sequence-tagged gene-trap library of >270,000 mouse embryonic stem cell clones representing mutations in ≈60% of mammalian genes. Through the generation and phenotypic analysis of knockout mice from this resource, we are undertaking a functional screen to identify genes regulating physiological parameters such as blood pressure. As part of this screen, mice deficient for the Wnk1 kinase gene were generated and analyzed. Genetic studies in humans have shown that large intronic deletions in WNK1 lead to its overexpression and are responsible for pseudohypoaldosteronism type II, an autosomal dominant disorder characterized by hypertension, increased renal salt reabsorption, and impaired K+ and H+ excretion. Consistent with the human genetic studies, Wnk1 heterozygous mice displayed a significant decrease in blood pressure. Mice homozygous for the Wnk1 mutation died during embryonic development before day 13 of gestation. These results demonstrate that Wnk1 is a regulator of blood pressure critical for development and illustrate the utility of a functional screen driven by a sequence-based mutagenesis approach.
Antiemetic efficacy of combined aprepitant and dexamethasone in patients at high-risk of postoperative nausea and vomiting from epidural fentanyl analgesia.

BACKGROUND Postoperative opioid analgesia increases the incidence of postoperative nausea and vomiting (PONV). We investigated whether a combination of the neurokinin-1 antagonist aprepitant and dexamethasone decreases PONV incidence compared with dexamethasone alone in high-risk patients receiving continuous epidural fentanyl. METHODS Sixty nonsmoking female patients scheduled for elective knee osteoarthritis surgery were randomly allocated to receive oral aprepitant 80 mg (aprepitant+dexamethasone group, N.=30) 2 h before anesthesia induction or no oral aprepitant (dexamethasone group, N.=30). All patients received intravenous dexamethasone 8 mg immediately before anesthesia induction. Anesthesia was maintained with remifentanil and sevoflurane. Continuous infusion of epidural analgesia, including fentanyl, was provided during and after surgery. We assessed complete response (no PONV and no rescue antiemetic use), incidence of nausea and vomiting, nausea severity scale, vomiting frequency, rescue antiemetic use, and postoperative pain at 2 and 24 h after surgery. RESULTS The cumulative incidence of vomiting at 24 h was 3% in the aprepitant+dexamethasone group and 27% in the dexamethasone group (P=0.011). The incidence and frequency of vomiting in the late postoperative period was also significantly lower in the aprepitant+dexamethasone group than in the dexamethasone group. However, there were no significant group differences in the proportion of patients who experienced a complete response, the incidence and severity of nausea, and rescue antiemetic use at 24 h. CONCLUSION The combination of aprepitant and dexamethasone was more effective in preventing postoperative vomiting compared with dexamethasone alone in patients at high-risk of PONV from continuous epidural fentanyl analgesia.
The Locator/ID Separation Protocol (LISP)

This document describes a network-layer-based protocol that enables separation of IP addresses into two new numbering spaces: Endpoint Identifiers (EIDs) and Routing Locators (RLOCs). No changes are required to either host protocol stacks or to the "core" of the Internet infrastructure. The Locator/ID Separation Protocol (LISP) can be incrementally deployed, without a "flag day", and offers Traffic Engineering, multihoming, and mobility benefits to early adopters, even when there are relatively few LISP-capable sites. Design and development of LISP was largely motivated by the problem statement produced by the October 2006 IAB Routing and Addressing Workshop. This document defines an Experimental Protocol for the Internet community.
Collective securitisation in the EU: normative dimensions

Abstract Discussions of collective political actors and normativity usually refer to the greater responsibility collectives (such as the EU) enjoy when acting upon the ills of the world either because such bodies are able to pool capabilities or because they enjoy the credibility of leading by example. Following a different line of argument, this article suggests that collective securitisation poses two hitherto unacknowledged normative issues. The first concerns the question whether just (morally permissible) collective securitisation requires unanimity, or second best, majority consensus on the need for, and the means of, securitisation by the constitutive member states of the collective. The second issue is related to individual states disaggregated from collective security actors. Specifically, ought those states culpable in threat creation be more liable for bearing the financial costs of collective securitisation?
Current sensorless solutions for PFC of boost converters with passivity-based and sliding mode control

In this paper current sensorless solutions for AC-DC boost converter Power Factor Control (PFC) have been presented. A Passivity-Based Control (PBC) and a Sliding Mode Control (SMC) with input voltage feedforward have been implemented to increase Power Factor (PF) and their performance has been compared. A robust current observer has been adopted in order to eliminate the need for expensive current sensors. The proposed solution has been numerically tested on a powerful software simulation platform.
Spontaneous patterning obtained by evaporation of Human Elastin-like Polypeptide solutions

The potential of producing patterned, bioactive coatings with Human Elastin-Like Polypeptides (HELPs) has been investigated. The physicochemical features of these compounds have evidenced some differences between the two recombinantly expressed products. By a device-free, simple route and avoiding the use of chemically unfriendly compounds, micropatterned surfaces with the ability to control cell behavior could be obtained. Thus, HELPs represent a very promising class of macromolecule for future applications in surface engineering.
Hyperhomocysteinemia: an independent risk factor for vascular disease.

BACKGROUND Hyperhomocysteinemia arising from impaired methionine metabolism, probably usually due to a deficiency of cystathionine beta-synthase, is associated with premature cerebral, peripheral, and possibly coronary vascular disease. Both the strength of this association and its independence of other risk factors for cardiovascular disease are uncertain. We studied the extent to which the association could be explained by heterozygous cystathionine beta-synthase deficiency. METHODS We first established a diagnostic criterion for hyperhomocysteinemia by comparing peak serum levels of homocysteine after a standard methionine-loading test in 25 obligate heterozygotes with respect to cystathionine beta-synthase deficiency (whose children were known to be homozygous for homocystinuria due to this enzyme defect) with the levels in 27 unrelated age- and sex-matched normal subjects. A level of 24.0 mumol per liter or more was 92 percent sensitive and 100 percent specific in distinguishing the two groups. The peak serum homocysteine levels in these normal subjects were then compared with those in 123 patients whose vascular disease had been diagnosed before they were 55 years of age. RESULTS Hyperhomocysteinemia was detected in 16 of 38 patients with cerebrovascular disease (42 percent), 7 of 25 with peripheral vascular disease (28 percent), and 18 of 60 with coronary vascular disease (30 percent), but in none of the 27 normal subjects. After adjustment for the effects of conventional risk factors, the lower 95 percent confidence limit for the odds ratio for vascular disease among the patients with hyperhomocysteinemia, as compared with the normal subjects, was 3.2. The geometric-mean peak serum homocysteine level was 1.33 times higher in the patients with vascular disease than in the normal subjects (P = 0.002). The presence of cystathionine beta-synthase deficiency was confirmed in 18 of 23 patients with vascular disease who had hyperhomocysteinemia. CONCLUSIONS Hyperhomocysteinemia is an independent risk factor for vascular disease, including coronary disease, and in most instances is probably due to cystathionine beta-synthase deficiency.