Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of heat shock on in vitro development and intracellular oxidative state of bovine preimplantation embryos

We investigated the effects of heat shock on developmental competence of bovine embryos and intracellular oxidative state. After in vitro fertilization, embryos were exposed to heat shock at 41°C for 6 hr on days 0, 2, 4, and 6, respectively. On day 2, cleavage rate was not significantly different in all groups. However, the percentage of embryos developing to blastocyst stage after exposure to heat shock on day 0 (18.8 ± 4.3%) and day 2 (23.6 ± 3.7%) were significantly decreased compared with control (37.5 ± 4.0%), day 4 (40.0 ± 7.4%), and day 6 (38.1 ± 2.0%). In addition, the total cell number of blastocysts was significantly decreased by heat shock on day 0 (107.5 ± 6.6) and day 2 (112.8 ± 5.7) compared with the control (143.2 ± 9.4). To evaluate intracellular oxidative state by heat shock, embryos exposed to heat shock on days 0, 2, 4, and 6 were incubated with 2′,7′‐dichlorodihydrofluorescein diacetate (DCHFDA) and fluorescence of oxidized DCHFDA by reactive oxygen species (ROS) was detected under fluorescent microscope. The intensity of fluorescence was significantly increased when embryos were exposed to heat shock on days 0 and 2. However, heat shock on day 4 and day 6 did not increase the fluorescence intensity. These results indicate that (1) heat shock to earlier stage embryos causes a decrease in development to blastocysts and cell proliferation and (2) the decrease in development by heat shock could be involved in an increase of intracellular oxidative stress. Mol. Reprod. Dev. 67: 77–82, 2004. © 2004 Wiley‐Liss, Inc.
Measurement of contrast sensitivity and glare under mesopic and photopic conditions following wavefront-guided and conventional LASIK surgery.

PURPOSE To compare contrast and glare vision in a prospective study of eyes treated using conventional and wavefront-guided LASIK surgery. The reproducibility of a glaremeter device used to quantitatively measure glare and halo was also determined. METHODS Ninety-two eyes of 46 patients underwent conventional LASIK surgery and 104 eyes of 52 patients underwent wavefront-guided LASIK surgery. Visual acuity, glare disability measured using a glaremeter, and contrast sensitivity assessed using a Pelli-Robson chart were measured monthly for 6 months postoperatively. Glaremeter testing was performed under both mesopic (5.4 +/- 0.4 cd/m2) and photopic (78.3 +/- 4.4 cd/m2) conditions. To evaluate the reproducibility of the glaremeter, 36 eyes of 18 nonoperated myopic patients were tested. RESULTS The coefficient of variation and the reliability coefficient for the glare test were 13.6% and 95.2%, respectively. The glaremeter showed that glare disability under mesopic conditions differed between conventional and wavefront-guided LASIK eyes over 6-month follow-up (907.5 +/- 491.5 vs 986.1 +/- 448.0 pixels preoperatively and 1717.1 +/- 521.2 vs 1407.8 +/- 411.3 pixels at 6 months, P<.0001). At 6 months, contrast sensitivity log values were 1.62 +/- 0.31 and 1.78 +/- 0.34 for conventional and wavefront-guided LASIK eyes, respectively (P=.010). The visual complaint score was lower in the wavefront-guided LASIK group (P=.0116). CONCLUSIONS Compared to conventional ablation, wavefront-guided ablation provided superior outcomes in terms of postoperative glare under mesopic conditions, subjective complaints, and contrast sensitivity. In addition, it appears the glaremeter can be used for clinical quantitative evaluation of glare and halo.
Hybrid Meta-Heuristics for Robust Scheduling

The production and delivery of rapidly perishable goods in distributed supply networks involves a number of tightly coupled decision and optimization problems regarding the just-in-time production scheduling and the routing of the delivery vehicles in order to satisfy strict customer specified time-windows. Besides dealing with the typical combinatorial complexity related to activity assignment and synchronization, effective methods must also provide robust schedules, coping with the stochastic perturbations (typically transportation delays) affecting the distribution process. In this paper, we propose a novel metaheuristic approach for robust scheduling. Our approach integrates mathematical programming, multi-objective evolutionary computation, and problem-specific constructive heuristics. The optimization algorithm returns a set of solutions with different cost and risk tradeoffs, allowing the analyst to adapt the planning depending on the attitude to risk. The effectiveness of the approach is demonstrated by a real-world case concerning the production and distribution of ready-mixed concrete.
Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

ABSTRACT Although clinical epidemiology lists human enteric viruses to be among the primary causes of acute gastroenteritis in the human population, their circulation in the environment remains poorly investigated. These viruses are excreted by the human population into sewers and may be released into rivers through the effluents of wastewater treatment plants (WWTPs). In order to evaluate the viral diversity and loads in WWTP effluents of the Paris, France, urban area, which includes about 9 million inhabitants (approximately 15% of the French population), the seasonal occurrence of astroviruses and noroviruses in 100 WWTP effluent samples was investigated over 1 year. The coupling of these measurements with a high-throughput sequencing approach allowed the specific estimation of the diversity of human astroviruses (human astrovirus genotype 1 [HAstV-1], HAstV-2, HAstV-5, and HAstV-6), 7 genotypes of noroviruses (NoVs) of genogroup I (NoV GI.1 to NoV GI.6 and NoV GI.8), and 16 genotypes of NoVs of genogroup II (NoV GII.1 to NoV GII.7, NoV GII.9, NoV GII.12 to NoV GII.17, NoV GII.20, and NoV GII.21) in effluent samples. Comparison of the viral diversity in WWTP effluents to the viral diversity found by analysis of clinical data obtained throughout France underlined the consistency between the identified genotypes. However, some genotypes were locally present in effluents and were not found in the analysis of the clinical data. These findings could highlight an underestimation of the diversity of enteric viruses circulating in the human population. Consequently, analysis of WWTP effluents could allow the exploration of viral diversity not only in environmental waters but also in a human population linked to a sewerage network in order to better comprehend viral epidemiology and to forecast seasonal outbreaks.
Patterns of agreement within the Noun Phrase

We focuse on plural agreement within the Noun Phrase in urban varieties of Portuguese, in the light of Theory of Variation and Change assumptions (Weinreich, Labov, Herzog, 1968) and based on the speech of individuals with fundamental, medium and superior education level, distributed, yet, for three age groups and sex. We suggest that nominal agreement is a categorical rule in European Portuguese and a variable rule in Brazilian Portuguese and in Sao Tome Portuguese, although these two varieties tend to adopt the canonical plural marking. The variable rule implementation is strongly determined by the linear and relative position of the constituent within the Noun Phrase and dependent on the action of external factors, among which education level, that seems congregate different aspects that characterize individuals from social point of view.
The intelligent ASIMO: system overview and integration

We present the system overview and integration of the ASIMO autonomous robot that can function successfully in indoor environments. The first model of ASIMO is already being leased to companies for receptionist work. In this paper, we describe the new capabilities that we have added to ASIMO. We explain the structure of the robot system for intelligence, integrated subsystems on its body, and their new functions. We describe the behavior-based planning architecture on ASIMO and its vision and auditory system. We describe its gesture recognition system, human interaction and task performance. We also discuss the external online database system that can be accessed using internet to retrieve desired information, the management system for receptionist work, and various function demonstrations.
Use of Geographically Weighted Regression Model for Exploring Spatial Patterns and Local Factors Behind NDVI-Precipitation Correlation

Normalized Difference Vegetation Index (NDVI)-precipitation correlation has long been studied. In previous studies, the correlation was usually based on global regression model, which assumed such correlation be constant across the space. However, NDVI-precipitation correlation is spatially dependent and affected by local factors (e.g., soil background). In this paper, geographically weighted regression model is utilized to analyze the NDVI-precipitation correlation on three land use types (i.e., 1) grassland, 2) fallow/idle land, and 3) winter wheat land) within U.S. central great plain area. Results suggest that geographically weighted regression model has better performances than global regression models. Specifically, higher average R2 (0.81) and lower proportion (9%) of residuals with spatial autocorrelation has been achieved under geographically weighted regression in comparison with lower average R2 (0.68) and higher proportion (38%) of residual with spatial autocorrelation under global regression models. In addition, the spatially dependent correlation between NDVI and precipitation has been revealed with geographically weighted regression model. From the north to south, the increasing unit rate of NDVI's change with precipitation has been found through spatially varying regression slopes. Moreover, local factors affecting NDVI-precipitation correlation, such as soil permeability and thickness, have been identified through analyzing the local goodness of fitting under geographically weighted regression model. In summary, unveiled spatial patterns of NDVI-precipitation correlation provide another perspective for studying correlations between NDVI and climatic factors. This work should also be helpful to better understand crop responses to precipitation in agricultural management.
Data collection procedures for school-based surveys among adolescents: the Youth in Europe Study.

BACKGROUND Collection of valid and reliable surveillance data as a basis for school health promotion and education policy and practice for children and adolescence is of great importance. However, numerous methodological and practical problems arise in the planning and collection of such survey data that need to be resolved in order to ensure the validity of the data and to maximize the response rate without being prohibitively costly. METHOD This article builds on a 15-yearlong experience of such annual data collections in Iceland and describes the preparation, process, and collection of data that provide a common methodologic framework for the school-based survey, Youth in Europe, a population-based survey of 14- to 16-year-old adolescents, being collected across 18 European cities now participating in the European Cities Against Drugs (ECAD) program. RESULTS We identified 11 critical steps for developing and implementing the surveys in light of the recent literature on the preparation and implementation practices in school-based data collection among adolescents. CONCLUSION Limiting the disruption of daily operations in schools while at the same time ensuring both quality and clarity of data collection procedures in school-based surveys are of paramount importance for researchers, school personnel, and students.
Fluorescence measurements of cytoplasmic and mitochondrial sodium concentration in rat ventricular myocytes.

1. The fluorescent Na+ indicator SBFI was incorporated into isolated ventricular myocytes using the acetoxymethyl (AM) ester. 2. The excitation spectrum was found to be shifted about 20 nm in the cell compared to in vitro. In the cell, an increase of [Na+] decreased fluorescence at 380 nm (F380) and had no effect at 340 nm (F340). The ratio (R = F340/F380) was used as a measure of [Na+]i. 3. In vivo calibration of SBFI for [Na+]i was obtained by equilibrating [Na+] across the plasma membrane with a divalent‐free solution in the presence of gramicidin D. 4. Selective removal of the surface membrane with saponin or digitonin released only about 50% of the indicator. Following saponin treatment, cyanide or carbonylcyanide m‐chlorphenylhydrazone (CCCP) increased the apparent [Na+] measured by the remaining (presumably mitochondrial) SBFI. It is suggested that mitochondrial [Na+] is normally less than cytoplasmic. 5. Attempts to examine the effects of metabolic inhibition on [Na+]i were hampered by changes of autofluorescence due to changes of [NADH]. It is shown that this effect can be corrected for using the isosbestic signal (excited at 340 nm). 6. Inhibition of both aerobic metabolism (with CN‐) and glycolysis (glucose removal or iodoacetate) produced a gradual increase of [Na+]i. This began before the resting contracture developed and may (via Na(+)‐Ca2+ exchange) account for some of the rise of diastolic [Ca2+]i seen in previous work. The rise of [Na+]i began at about the same time as the decrease of systolic contraction and therefore at a time when [ATP]i had begun to fall.
How Communication Technologies Function as Platforms and Pathways to Civic and Political Engagement: An Agenda for Research and Public Policy

This paper is an assessment of what we know empirically about how communication technologies are being used by young people (typically defined as those between the ages of 18 and 29) as both platforms and pathways for civic and political engagement. An overview of existing research is utilized as the basis for this investigation. Research on this topic is limited in several ways, including its failure to acknowledge the distinction between individuals who are engaged merely by using communication technologies (technology as a platform for participation) versus those who are engaged beyond the exclusive use of communication technologies (technology as a pathway for participation). Understanding this distinction can better enable scholars, policy-makers, and practitioners to develop inclusive strategies for engaging young people. The authors’ analysis reveals that recent research demonstrates that new technologies can serve as both platforms and pathways for civic and political engagement, and, with this distinction in mind, they provide recommendations to policyand decisionmakers and scholars interested in this issue. KEywoRdS Civic Engagement, Internet, Millennial Generation, Pathway, Platform, Political Engagement, Social Networking Site, Twitter
A thermal gel depot for local delivery of paclitaxel to treat experimental brain tumors in rats.

OBJECT Paclitaxel, a cellular proliferation inhibitor/radiation sensitizer, while effective against gliomas in vitro, has poor CNS penetration and dose-limiting toxicities when administered systemically. OncoGel (paclitaxel in Re-Gel) provides controlled local paclitaxel release when placed into the CNS. The authors evaluated the safety and efficacy of OncoGel in rats with intracranial 9L gliosarcoma. METHODS Safety studies included intracranial delivery of increasing volumes of ReGel and OncoGel containing 1.5 (OncoGel 1.5) or 6.3 (OncoGel 6.3) mg/ml paclitaxel. An in vivo radiolabeled biodistribution study was performed in 18 Fischer-344 rats to determine intracerebral distribution. Efficacy studies compared overall survival for controls, ReGel only, radiation therapy only, OncoGel 6.3, or OncoGel 6.3 in combination with radiation therapy. ReGel and OncoGel 6.3 were delivered either simultaneously with tumor implantation (Day 0) or 5 days later (Day 5). Radiation therapy was given on Day 5. RESULTS Control and ReGel animals died of tumor within 17 days. Survival significantly increased in the Onco-Gel 6.3 group on Day 0 (median 31 days; p = 0.0001), in the OncoGel 6.3 group on Day 5 (median 17 days; p = 0.02), and in the radiation therapy-only group (median 26 days; p = 0.0001) compared with controls. Animals receiving both OncoGel and radiation therapy had the longest median survival: 83 days in the group with radiation therapy combined with OncoGel 6.3 on Day 0, and 32 days in the group combined with OncoGel 6.3 on Day 5 (p = 0.0001 vs controls). After 120 days, 37.5% of the animals in the OncoGel Day 0 group, 37.5% of animals in the OncoGel 6.3 Day 0 in combination with radiation therapy group, and 12.5% of the animals in the OncoGel 6.3 on Day 5 in combination with radiation therapy group were alive. In the biodistribution study, measurable radioactivity was observed throughout the ipsilateral hemisphere up to 3 weeks after the OncoGel injection, with the most radioactivity detected 3 hours after injection. The highest dose of radioactivity observed in the contralateral hemisphere was at the Day 3 time point. CONCLUSIONS OncoGel containing 6.3 mg/ml of paclitaxel is safe for intracranial injection in rats and effective when administered on Day 0. When combined with radiation therapy, the combination was more effective than either therapy alone and should be studied clinically for the treatment of malignant glioma.
Human ABC transporters ABCG2 (BCRP) and ABCG4

1. The human ABC transporter ABCG2 is regarded as a member of the phase III system for xenobiotic metabolism, and it has been suggested that this efflux pump is responsible for protecting the body from toxic xenobiotics and for removing metabolites. 2. This review paper will address the new aspects of ABCG2 in terms of post-translational modifications (i.e., disulfide bond formation, ubiquitination, and endoplasmic reticulum-associated degradation) of ABCG2 protein, high-speed screening, and quantitative structure–activity relationship (QSAR) analysis to evaluate ABCG2–drug interactions, and genetic polymorphisms potentially associated with photosensitivity. 3. In addition, new aspects of human ABCG4 and mouse Abcg4 are presented with respect to their molecular properties and potential physiological roles. Considering a high sequence similarity between ABCG1 and ABCG4, both Abcg4 and ABCG4 may be involved in the transport of cholesterol from neurons and astrocytes. Furthermore, high expression of the mouse Abcg4 protein in the testis implicates its involvement in transport of certain sex hormones.
PAM-DMT for Intensity-Modulated and Direct-Detection Optical Communication Systems

A novel concept of pulse-amplitude-modulated discrete multitone modulation (PAM-DMT) is presented. It is shown that by pulse amplitude modulation of the subcarriers used, the resulting PAM-DMT time signal can be clipped asymmetrically to achieve a unipolar signal for transmission. The distortion resulting from this asymmetric clipping is orthogonal to the information signal itself, causing no additional penalty. Especially in intensity-modulated direct-detection optical channels, where a unipolar signal is to be transmitted, it is shown that this idea can improve system performance.
A Forgotten Minority? A Content Analysis of Asian Pacific Americans in Introductory American Government Textbooks

ABSTRACT Textbooks are the most important pedagogical tools in higher education and they should convey sufficient and accurate information on minority groups and women in the United States. Yet textbooks tend to marginalize these groups in their depictions. This article examines the coverage of Asian Pacific Americans in twenty-eight American Government or Politics textbooks. Asian Pacific Americans have faced a unique history of exclusion, discrimination, and stereotyping. The content analysis of the textbooks reveals that textbooks do not fully cover their history and contributions to US politics, either measured by page numbers or by historical events and figures important to Asian Pacific Americans. To rectify this lack of coverage, this article concludes with five constructive recommendations, including an option to invite scholars on Asian Pacific American politics to serve as textbook reviewers and textbook coauthors.
Map making by cooperating mobile robots

The problem of map making using cooperating multiple heterogeneous mobile robots is investigated. The mobile robots vary in size and capabilities in terms of speeds to navigate through the region and sensor ranges to acquire information about the region. The robots are assumed to have sufficient memory to store the map and to be able to communicate with each other. An algorithm is presented for map making by multiple mobile robots in a cooperative manner. The authors' approach makes use of an occupancy grid and completes a map to a specified resolution. The algorithm is discussed in detail, and its feasibility is demonstrated by simulation results for the case of two cooperating mobile robots. <<ETX>>
Adding multiple micronutrient powders to a homestead food production programme yields marginally significant benefit on anaemia reduction among young children in Nepal.

Anaemia affects 46% of preschool-aged children in Nepal. A cluster-randomised study was conducted in rural Nepal to test whether providing micronutrient powders (MNP) in addition to enhanced homestead food production (EHFP) programme, consisting of home gardens, poultry and nutrition education, could lead to a higher reduction in anaemia compared with providing only EHFP. This sub-study enrolled 335 children aged 6-9 months into one of three groups: (1) EHFP + MNP; (2) EHFP; or (3) control. The EHFP + MNP group received 60 sachets of MNP for flexible consumption at the start and 6 months later for a total supplementation period of 11 months. The MNP contained 15 micronutrients including iron (10 mg encapsulated ferrous fumarate). Haemoglobin and anthropometry were measured at baseline and post-MNP supplementation. Mean ± SE haemoglobin concentration increased significantly in all groups, with a slightly higher but non-significant increase in the EHFP + MNP and EHFP compared with control (difference-in-differences: 4.1 g L(-1) for EHFP + MNP vs. control; 3.6 g L(-1) for EHFP vs. control; 0.5 g L(-1) for EHFP + MNP vs. EHFP). Anaemia decreased at a slightly higher magnitude in the EHFP + MNP [51.5 percentage points (PP)] than the EHFP (48.6 PP) and control (39.6 PP), with adjusted odds ratios (95% CI) at post-supplementation of 0.52 (0.25-1.12) for EHFP + MNP and 0.69 (0.35-1.36) for EHFP, compared with control. There was no impact on child growth. Combining EHFP and MNP programmes yielded a marginally significant reduction in anaemia among children.
Why Do Firms Purchase Used Assets?

We hypothesize that the increase in expectation about future technological change decreases the likelihood for the purchase of new assets because the change may make the new assets obsolete. To test this hypothesis, we use patents and citations data to represent the expected technological change. We find that the purchases of and the amount spent on used assets increase with the expectation of technological change, while time to completion of the purchase and the number of bids decrease with the increase in expectations about technological change. We exploit industry deregulations to establish identification. In contrast to the literature, we find no empirical support for financial constraint as a reason for purchasing used assets.