Miracle-dz/UniFAR-FTUs huggingface dataset:
Inter- and intratester reliability values of ultrasound imaging measurements of diaphragm movement in the thoracic and thoracolumbar curves in adolescent idiopathic scoliosis

ABSTRACT Purpose: The purpose of this study was to determine intertester and intratester reliability of ultrasound measurements of bilateral diaphragm excursions in the thoracic and thoracolumbar spinal curves of 31 females with adolescent idiopathic scoliosis (AIS) (mean age = 14.1 ± 1.8 years). Method: Subjects were tested during tidal breathing using real-time ultrasound imaging with a 3.5 MHz curvilinear transducer. Results: There were no significant differences in intratester and intertester reliability values in bilateral diaphragmatic excursions measured at the thoracolumbar spinal curve, whereas significant differences were observed in measurements taken at the thoracic spinal curve (p < 0.05). Overall, the intertester and intratester reliabilities of the thoracic and thoracolumbar curves in AIS ranged from 0.764 to 0.998. Conclusions: These findings suggest that ultrasound imaging is highly reliable between and within testers and is useful to precisely discriminate pathological diaphragm movement in idiopathic thoracic scoliosis and idiopathic thoracolumbar scoliosis.
It Attacked My Change: An Exploratory Study with Young Adults on the Impact of Divorce and Their Adjustment Processes During Adolescence

Semistructured interviews were conducted with 2 female students, 21 and 24 years old. The participants experienced parental divorce during early adolescence (12 years old). This research examined the participants’ own views on the impact of parental divorce and their adjustment processes. The majority of participants’ narratives indicated that they had experienced negative effects of parental divorce. Results suggested that their adjustment was a long process in which mothers, peers, and psychologists had an important role in their coping process. Divorce and associated events were found to have a direct impact on participants’ development of identity, emotions, intimate relationships, father–child relationships, and views about forming their own families. The results were discussed in relation to the previous literature. Additionally, areas for psychotherapeutic emphasis are presented.
Embryonic hair follicle fate change by augmented β-catenin through Shh and Bmp signaling

β-catenin signaling is one of the key factors regulating the fate of hair follicles (HFs). To elucidate the regulatory mechanism of embryonic HF fate determination during epidermal development/differentiation, we analyzed conditional mutant mice with keratinocytes expressing constitutively activeβ -catenin (K5-Cre Catnb(ex3)fl/+). The mutant mice developed scaly skin with a thickened epidermis and showed impaired epidermal stratification. The hair shaft keratins were broadly expressed in the epidermis but there was no expression of the terminal differentiation markers K1 and loricrin. Hair placode markers (Bmp2 and Shh) and follicular dermal condensate markers (noggin, patched 1 and Pdgfra) were expressed throughout the epidermis and the upper dermis, respectively. These results indicate that the embryonic epidermal keratinocytes have switched extensively to the HF fate. A series of genetic studies demonstrated that the epidermal switching to HF fate was suppressed by introducing the conditional mutation K5-Cre Catnb(ex3)fl/+Shhfl/- (with additional mutation of Shh signaling) or K5-Cre Catnb(ex3)fl/+BmprIAfl/fl (with additional mutation of Bmp signaling). These results demonstrate that Wnt/β-catenin signaling relayed through Shh and Bmp signals is the principal regulatory mechanism underlying the HF cell fate change. Assessment of Bmp2 promoter activities suggested a putative regulation by β-catenin signaling relayed by Shh signaling towards Bmp2. We also found that Shh protein expression was increased and expanded in the epidermis of K5-Cre Catnb(ex3)fl/+BmprIAfl/fl mice. These results indicate the presence of growth factor signal cross-talk involving β-catenin signaling, which regulates the HF fate.
Scalable Computing : Practice and Experience

The importance of distributed applications is constantly rising due to technological trends such as the widespread usage of smart phones and the increasing internetworking of all kinds of devices. In addition to classical application scenarios with a rather static structure these trends push forward dynamic settings, in which service providers may continuously vanish and newly appear. In this paper categories of distributed applications are identified and analyzed with respect to their most important development challenges. In order to tackle these problems already on a conceptual level the active component paradigm is proposed, bringing together ideas from agents, services and components using a common conceptual perspective. Besides conceptual foundations of active components also a programming model and an implemented infrastructure are presented. It is highlighted how active components help addressing the initially posed challenges by presenting several real world example applications.
Dietary nitrite supplementation protects against myocardial ischemia-reperfusion injury

Nitrite has emerged as an endogenous signaling molecule with potential therapeutic implications for cardiovascular disease. Steady-state levels of nitrite are derived in part from dietary sources; therefore, we investigated the effects of dietary nitrite and nitrate supplementation and deficiency on NO homeostasis and on the severity of myocardial ischemia-reperfusion (MI/R) injury. Mice fed a standard diet with supplementation of nitrite (50 mg/liter) in their drinking water for 7 days exhibited significantly higher plasma levels of nitrite, exhibited significantly higher myocardial levels of nitrite, nitroso, and nitrosyl–heme, and displayed a 48% reduction in infarct size (Inf) after MI/R. Supplemental nitrate (1 g/liter) in the drinking water for 7 days also increased blood and tissue NO products and significantly reduced Inf. A time course of ischemia-reperfusion revealed that nitrite was consumed during the ischemic phase, with an increase in nitroso/nitrosyl products in the heart. Mice fed a diet deficient in nitrite and nitrate for 7 days exhibited significantly diminished plasma and heart levels of nitrite and NO metabolites and a 59% increase in Inf after MI/R. Supplementation of nitrite in the drinking water for 7 days reversed the effects of nitrite deficiency. These data demonstrate the significant influence of dietary nitrite and nitrate intake on the maintenance of steady-state tissue nitrite/nitroso levels and illustrate the consequences of nitrite deficiency on the pathophysiology of MI/R injury. Therefore, nitrite and nitrate may serve as essential nutrients for optimal cardiovascular health and may provide a treatment modality for cardiovascular disease.
Quantitative assessment of DNA microarrays--comparison with Northern blot analyses.

DNA microarray is a powerful technology that provides the expression profile of thousands of genes. However, less attention has been paid to its quantitative aspect. In this study, we constructed a small-scale DNA microarray that contains 84 genes and characterized its quantitative aspect. Analyses with this microarray showed that 17 genes were induced, whereas 8 genes were suppressed at least twofold during the differentiation of mouse embryonic stem cells. When repeated with the same combination of fluorescent dyes for probe labeling, the microarray produced consistent data (correlation coefficient = 0.991). In contrast, data were less consistent when repeated with the reverse combination of dyes (correlation coefficient = 0.945). The effect of dye combination was particularly evident in several genes. Total RNA (15 microg) and poly(A) RNA (0.5 microg) showed comparable sensitivity and produced essentially identical data (correlation coefficient = 0.983). The sensitivity of the DNA microarrays was slightly inferior to that of Northern blot analyses. In most genes, data obtained with the two methods were consistent. However, in 4 of 46 genes compared, DNA microarrays failed to detect the expression changes that were revealed by Northern blot. These data demonstrated that DNA microarrays provide quantitative data comparable to Northern blot in general, but a few issues must be considered when analyzing data.
Prejudice, politics, and public opinion : Understanding the sources of racial policy attitudes

This review examines the intersection of prejudice, politics, and public opinion. It focuses specifically on research that seeks to understand the sources of attitudes toward policies intended to benefit African Americans and other racial/ethnic minorities by ensuring equal treatment, providing opportunity enhancement, or striving for equal outcomes. After a review of the main patterns of white and African-American public opinion on this topic, three central theoretical interpretations of racial policy attitudes—new racism, politics and nonracial principles and values, and group conflict theories—are described and compared. The empirical evidence for each approach is assessed. Finally, directions of research that pursue a more complex view of racial policy attitudes are introduced. These include efforts to incorporate insights across theoretical domains as well as correcting an overemphasis on cognitive issues to the exclusion of affect. In addition, gaps in our understanding of “non-white” attitudes, non.
ATM controls proper mitotic spindle structure

The recessive ataxia-telangiectasia (A-T) syndrome is characterized by cerebellar degeneration, immunodeficiency, cancer susceptibility, premature aging, and insulin-resistant diabetes and is caused by loss of function of the ATM kinase, a member of the phosphoinositide 3-kinase–like protein kinases (PIKKs) family. ATM plays a crucial role in the DNA damage response (DDR); however, the complexity of A-T features suggests that ATM may regulate other cellular functions. Here we show that ATM affects proper bipolar mitotic spindle structure independently of DNA damage. In addition, we find that in mitosis ATM forms a complex with the poly(ADP)ribose (PAR) polymerase Tankyrase (TNKS) 1, the spindle pole protein NuMA1, and breast cancer susceptibility protein BRCA1, another crucial DDR player. Our evidence indicates that the complex is required for efficient poly(ADP)ribosylation of NuMA1. We find further that a mutant NuMA1 version, non-phosphorylatable at potential ATM-dependent phosphorylation sites, is poorly PARylated and induces loss of spindle bipolarity. Our findings may help to explain crucial A-T features and provide further mechanistic rationale for TNKS inhibition in cancer therapy.
Transforming Former State Enterprises in the Czech Republic

The study of organizational transformation has emerged from the foundations established by contingency theory and research. While institutional approaches to organizational analysis have preferred to focus on the tendency towards organizational continuity and inertia, recent developments have begun to con sider institutional pressures leading to change, and to provide clues about how contingency and institutional theories might complement each other in improv ing our understanding of organizational change. The evidence presented in this paper, drawn from a study of organizational transformation in the Czech Republic, allows exploration of the relationship between transforming state enterprises and the wider processes of social, economic and institutional change. The values, motives and actions of the key enterprise managers are shown to be essential factors in explaining both the process of transformation in state enterprises, and the role of institutional factors in that process.
The prescribing of generic medicines in Nigeria: knowledge, perceptions and attitudes of physicians

ABSTRACT Generic medicines have the same efficacy and safety as originators at lower prices; however, there are concerns with their utilization in Nigeria. Objective was to evaluate physicians’ understanding and perception of generics. A questionnaire was administered among physicians working in tertiary healthcare facilities in four geo-political regions of Nigeria. Questionnaire response was 74.3% (191/257) among mainly males (85.9%). The mean knowledge score regarding generics was 5.3 (maximum of 9) with 36.6%, 36.1% and 27.2% having poor, average and good knowledge respectively. Cross-tabulation showed statistical significance (p = 0.047) with the duration of practice but not with position, subspecialty or sex. The majority of respondents did not believe that generic medicines are of lower quality than branded medicines. Therapeutic failure was a major concern in 82.7%, potentially discouraging the prescribing of generics, and a majority (63.9%) did not support generic substitution by pharmacists. Knowledge gaps were identified especially with the perception of generics, which need to be addressed.
Natural Competence in the Hyperthermophilic Archaeon Pyrococcus furiosus Facilitates Genetic Manipulation: Construction of Markerless Deletions of Genes Encoding the Two Cytoplasmic Hydrogenases

ABSTRACT In attempts to develop a method of introducing DNA into Pyrococcus furiosus, we discovered a variant within the wild-type population that is naturally and efficiently competent for DNA uptake. A pyrF gene deletion mutant was constructed in the genome, and the combined transformation and recombination frequencies of this strain allowed marker replacement by direct selection using linear DNA. We have demonstrated the use of this strain, designated COM1, for genetic manipulation. Using genetic selections and counterselections based on uracil biosynthesis, we generated single- and double-deletion mutants of the two gene clusters that encode the two cytoplasmic hydrogenases. The COM1 strain will provide the basis for the development of more sophisticated genetic tools allowing the study and metabolic engineering of this important hyperthermophile.
Low‐intensity pulsed ultrasound (LIPUS) increases the articular cartilage type II collagen in a rat osteoarthritis model

In this study, the effect of low‐intensity pulsed ultrasound (LIPUS) on cartilage was evaluated in a rat osteoarthritis (OA) model using serum biomarkers such as CTX‐II (type II collagen degradation) and CPII (type II collagen synthesis) as well as histological criteria (Mankin score and immunohistochemical type II collagen staining). OA was surgically induced in the knee joint of rats by anterior cruciate/medial collateral ligament transection and medial meniscus resection (ACLT + MMx). Animals were divided into three groups: sham‐operated group (Sham), ACLT + MMx group without LIPUS (−LIPUS), and ACLT + MMx group with LIPUS (+LIPUS; 30 mW/cm2, 20 min/day for 28 days). CTX‐II levels were elevated in both −LIPUS and +LIPUS groups compared to that in the Sham group after the operation, but there was no significant difference between +LIPUS and −LIPUS groups, suggesting that LIPUS does not affect the degradation of type II collagen in this model. In contrast, CPII was significantly increased in +LIPUS group compared to −LIPUS and Sham. Moreover, histological damage on the cartilage (Mankin score) was ameliorated by LIPUS, and type II collagen was immunohistochemically increased by LIPUS in the cartilage of an OA model. Of interest, mRNA expression of type II collagen was enhanced by LIPUS in chondrocytes. Together these observations suggest that LIPUS is likely to increase the type II collagen synthesis in articular cartilage, possibly via the activation of chondrocytes and induction of type II collagen mRNA expression, thereby exhibiting chondroprotective action in a rat OA model. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:361–369, 2010
Limitations of reduced‐field irradiated volume and technique in conventional radiation therapy of prostate cancer: Implications for conformal 3‐D treatment

In order to define technical limitations of conventional external beam irradiation for clinically localized prostate cancer, we evaluated the impact of several reduced‐field treatment factors, such as reduced‐field (RF) irradiated volume, RF technique, photon energy of treatment, and dose on survival endpoints and local control in a retrospective series. Several survival endpoints, such as disease‐specific survival, freedom from relapse survival, biochemical no‐evidence of disease (bNED) survival, and local control were associated with several treatment variables using univariate and multivariate analyses in 329 patients. Reduced‐field technique appeared to predict survival outcome, with patients treated by bilateral 120° arcs faring less well than those treated by full 360° rotational fields. The irradiated volume of the reduced‐field was also significantly associated with survival outcome, with patients treated with smaller volumes faring less well. Local failure rates also appeared increased, although not statistically, in patients treated with smaller RF sizes. In an attempt to explain these detected deficiencies, dose‐volume histograms for prostate coverage were created for a small sample of patients. The deficiencies related to small reduced‐field volume appeared to be largely attributable to poor dosimetric coverage of the prostate. These results underscore the limitations of conventional external beam treatment for prostate carcinoma when conventional techniques are employed, particularly if small reduced fields are used, and further supports the development of improved treatment techniques, such as conformal irradiation, as alternatives. Int. J. Cancer (Radiat. Oncol. Invest.) 90:265–274, 2000. © 2000 Wiley‐Liss, Inc.
Twitter postings and suicide: An analysis of the postings of a fatal suicide in the 24 hours prior to death

In the 24 hours prior to her death by suicide, a young girl made 145 posts on Twitter. In the posts, she outlines her history of incestuous sexual abuse, rape, and risky sexual behavior. These posts were analyzed using the Linguistic Inquiry and Word Count (LIWC). Trends were found indicating an increase in positive emotions over the 24 hours and a change in focus from the self to others. This first study of posts on Twitter by a person about to commit suicide provides hypotheses for future studies of this medium for communication.
Training nurses for interdisciplinary communication with families in the intensive care unit: an intervention.

BACKGROUND Critical care nurse communication training has largely been limited to didactic materials, interactive training for nurse supervisors, or brief participatory learning programs within the context of comprehensive end-of-life care educational seminars. Preliminary evidence suggests that an interactive approach can also be effective in communication skills training for intensive care unit (ICU) nurses. METHODS We implemented a 1-day educational intervention in five acute care hospitals within Veterans Integrated Service Network (VISN) 3 (New York-New Jersey region) of the Department of Veterans Affairs and focused solely on communication skills and targeted specifically to nurses providing bedside care for critically ill patients. A "learner centered" approach to skills training that has several integral components was employed. AMONG THESE ARE: a cognitive, evidence-based foundation upon which to build new skills; a method such as role-play that allows participants to practice newly learned skills; and an affective component, during which trainees can freely discuss their impressions of the exercise or explore difficulties that may have been encountered. Before and after the program we conducted a detailed assessment of participants' self-rated communication skills and of the techniques and materials we used. RESULTS AND CONCLUSIONS Post-program responses documented significant improvement in self-evaluated skills for each of the core tasks we assessed. Evidence suggests that communication with patients and families in the ICU can be most effectively approached in an interdisciplinary way. For nurses to fully realize their potential for optimal communication as members of the multidisciplinary team, they must be equipped with the necessary skills. We believe this new program helps to expand the range of approaches for training nurses in essential communication skills.
Stand By Me: The Presence Of A Close Friend Helps Against The Negative Effects Of Social Exclusion

Individuals are highly attuned to cues of exclusion, even when it is not caused by an actual social threat. To date there is little evidence identifying the situational factors that could buffer individuals from the detrimental consequences of exclusion. Our study investigated how the presence of a close friend, a potential attachment figure, affects the perception and experience of one-person exclusion. Participants played Cyberball either in the presence of a friend or alone. They were either included by both players or excluded by one player while the other remained fair. Although all excluded participants detected, participants who were excluded in the presence of their friends reported significantly better mood-and-need levels compared to those who participated alone. Individual differences did not moderate the effects. These findings suggest that although it does not affect the perception of social exclusion, the presence of a friend could buffer against the negative effects of exclusion.
Two Agents in the Brain: Motor Control of Unimanual and Bimanual Reaching Movements

Previous studies have suggested that the left and right hands have different specialties for motor control that can be represented as two agents in the brain. This study examined how coordinated movements are performed during bimanual reaching tasks to highlight differences in the characteristics of the hands. We examined motor movement accuracy, reaction time, and movement time in right-handed subjects performing a three-dimensional motor control task (visually guided reaching). In the no-visual-feedback condition, right-hand movement had lower accuracy and a shorter reaction time than did left-hand movement, whereas bimanual movement had the longest reaction time, but the best accuracy. This suggests that the two hands have different internal models and specialties: closed-loop control for the right hand and open-loop control for the left hand. Consequently, during bimanual movements, both models might be used, creating better control and planning (or prediction), but requiring more computation time compared to the use of one hand only.