Miracle-dz/UniFAR-FTUs huggingface dataset:
Telomerase reverse transcriptase protects against angiotensin II-induced microvascular endothelial dysfunction.

A rise in reactive oxygen species (ROS) may contribute to cardiovascular disease by reducing nitric oxide (NO) levels, leading to loss of NO's vasodilator and anti-inflammatory effects. Although primarily studied in larger conduit arteries, excess ROS release and a corresponding loss of NO also occur in smaller resistance arteries of the microcirculation, but the underlying mechanisms and therapeutic targets have not been fully characterized. We examined whether either of the two subunits of telomerase, telomerase reverse transcriptase (TERT) or telomerase RNA component (TERC), affect microvascular ROS production and peak vasodilation at baseline and in response to in vivo administration to angiotensin II (ANG II). We report that genetic loss of TERT [maximal dilation: 52.0 ± 6.1% with vehicle, 60.4 ± 12.9% with Nω-nitro-l-arginine methyl ester (l-NAME), and 32.2 ± 12.2% with polyethylene glycol-catalase (PEG-Cat) ( P < 0.05), means ± SD, n = 9-19] but not TERC [maximal dilation: 79 ± 5% with vehicle, 10.7 ± 9.8% with l-NAME ( P < 0.05), and 86.4 ± 8.4% with PEG-Cat, n = 4-7] promotes flow-induced ROS formation. Moreover, TERT knockout exacerbates the microvascular dysfunction resulting from in vivo ANG II treatment, whereas TERT overexpression is protective [maximal dilation: 88.22 ± 4.6% with vehicle vs. 74.0 ± 7.3% with ANG II (1,000 ng·kg-1·min-1) ( P = not significant), n = 4]. Therefore, loss of TERT but not TERC may be a key contributor to the elevated microvascular ROS levels and reduced peak dilation observed in several cardiovascular disease pathologies. NEW & NOTEWORTHY This study identifies telomerase reverse transcriptase (TERT) but not telomerase RNA component as a key factor regulating endothelium-dependent dilation in the microcirculation. Loss of TERT activity leads to microvascular dysfunction but not conduit vessel dysfunction in first-generation mice. In contrast, TERT is protective in the microcirculation in the presence of prolonged vascular stress. Understanding the mechanism of how TERT protects against vascular stress represents a novel target for the treatment of vascular disorders.
Rapid Chiral Assembly of Rigid DNA Building Blocks for Molecular Nanofabrication

Practical components for three-dimensional molecular nanofabrication must be simple to produce, stereopure, rigid, and adaptable. We report a family of DNA tetrahedra, less than 10 nanometers on a side, that can self-assemble in seconds with near-quantitative yield of one diastereomer. They can be connected by programmable DNA linkers. Their triangulated architecture confers structural stability; by compressing a DNA tetrahedron with an atomic force microscope, we have measured the axial compressibility of DNA and observed the buckling of the double helix under high loads.
Influence of Catastrophizing on Treatment Outcome in Patients With Nonspecific Low Back Pain: A Systematic Review

Study Design. Systematic review. Objective. The aim of this study was to assess the effect of catastrophizing on treatment efficacy and outcome in patients treated for low back pain. Summary of Background Data. Psychological factors including catastrophizing thoughts are thought to increase the risk for chronic low back pain. The influence of catastrophizing is debated. Methods. In September 2012, the following databases were searched: BIOSIS, CINAHL, Cochrane Library, EMBASE, OTseeker, PeDRO, PsycINFO, MEDLINE, Scopus, and Web of Science. For 50 of 706 references, full text was assessed. Results based on 11 studies were included in this analysis. Results. In the 11 studies, a total of 2269 patients were included. Seven studies were of good and 4 of moderate methodological quality. Heterogeneity in study settings, treatments, outcomes, and patient populations impeded meta-analysis. Catastrophizing at baseline was predictive for disability at follow-up in 4 studies and for pain in 2 studies. Three studies found no predictive effect of catastrophizing. A mediating effect was found in all studies (n = 5) assessing the impact of a decrease in catastrophizing during treatment. A greater decrease was associated with better outcome. Most studies that investigated the moderating effects on treatment efficacy found no effect (n = 5). However, most studies did not look for a direct interaction between the treatment and catastrophizing thoughts. No study investigated the influence of catastrophizing on work-related outcomes including return to work. Conclusion. Catastrophizing predicted degree of pain and disability and mediated treatment efficacy in most studies. The presence of catastrophizing should be considered in patients with persisting back pain. Limited evidence was found for the moderating effects on treatment efficacy. Future research should aim to clarify the role of catastrophizing as a moderator of outcome and investigate its importance for work-related outcomes. Level of Evidence: 1
APOYO SOCIAL PERCIBIDO EN HOMBRES OPERADOS DE CÁNCER DE LARINGE

It is studied the level of perceived social support in a sample of male subjects operated for larynx cancer by conducting comparisons with a sample of healthy males and assessing the perception of the partners of both groups of men. The objectives are to assess the perceived social support by men operated for larynx cancer and establish the differences with respect to the perceived social support between the group of healthy males and that of the cancer patients. The sample consisted of 25 males operated for larynx cancer, 25 healthy males, 15 female partners of the males diagnosed with cancer and 15 female partners of the healthy males. To assess perceived social support, we used the ISEL of Cohen et al (1983). Our main fi nding was that laryngectomized males perceive informational support as the least accessible to them, while they perceive instrumental and emotional support the most. Between laryngectomized and healthy males we only found differences in relation to the perception of social support of an instrumental nature.
Efficacy of a Gluten-Free Diet in the Gilles de la Tourette Syndrome: A Pilot Study

The Gilles de la Tourette syndrome (GTS) and Non-Coeliac Gluten Sensitivity (NCGS) may be associated. We analyse the efficacy of a gluten-free diet (GFD) in 29 patients with GTS (23 children; six adults) in a prospective pilot study. All of them followed a GFD for one year. The Yale Global Tics Severity Scale (YGTSS), the Yale-Brown Obsessive-Compulsive Scale—Self Report (Y-BOCS) or the Children’s Yale-Brown Obsessive-Compulsive Scale—Self Report (CY-BOCS), and the Cavanna’s Quality of Life Questionnaire applied to GTS (GTS-QOL) were compared before and after the GFD; 74% of children and 50% of adults were males, not significant (NS). At the beginning of the study, 69% of children and 100% of adults had associated obsessive-compulsive disorder (OCD) (NS). At baseline, the YGTSS scores were 55.0 ± 17.5 (children) and 55.8 ± 19.8 (adults) (NS), the Y-BOCS/CY-BOCS scores were 15.3, (standard deviation (SD) = 12.3) (children) and 26.8 (9.2) (adults) (p = 0.043), and the GTS-QOL scores were 42.8 ± 18.5 (children) and 64 ± 7.9 (adults) (p = 0.000). NCGS was frequent in both groups, with headaches reported by 47.0% of children and 83.6% of adults (p = 0.001). After one year on a GFD there was a marked reduction in measures of tics (YGTSS) (p = 0.001), and the intensity and frequency of OCD (Y-BOCS/CY-BOCS) (p = 0.001), along with improved generic quality of life (p = 0.001) in children and adults. In conclusion, a GFD maintained for one year in GTS patients led to a marked reduction in tics and OCD both in children and adults.
Regulation of tumor growth by synthetic disintegrins or depletion of PIN1

Schneider, R.A. , and Kinder, D.H. , The Role of Celecoxib in Cell Death. Am J Pharm Educ 68(2) Article 54, 2004. Kinder, D.H. , Schneider, R.A. , and Aulthouse, A.L. , Celecoxib (Celebrex®) induces apoptosis in MCF-7, BT-20, (breast carcinomas) A-375 (melanoma) and HT-29 (colorectal carcinoma) but does not activate Caspases 3, 8, or 9 in cells grown in monolayer. Proc Amer Assoc Cancer Res 46: 2687, 2005. Schneider, R.A. , and Hoyt, D.G. , Angiotensin II or Leptin induce cyclooxygenase-2, but are mutually antagonistic in mouse aortic endothelial cells. FASEB J. 21:878.12. 2007. Schneider, R.A. , Stevens, R.L. , and Hoyt, D.G. , Soluble anti-beta1 integrin antibody induces phosphorylation of focal adhesion kinase and protein kinase B/AKT in mouse aortic endothelial cells. FASEB J. 21:872.5. 2007. Schneider, R.A. , Liu, T. , Huang, Y. , Keshvara, L. , and Hoyt, D.G. , Depletion of Protein Interacting with Never in Mitosis gene A-1 (PIN1) decreases mammary adenocarcinoma cell growth in culture, but increases VEGF and tumor growth in mice. FASEB J. 22:321.8. 2008. Hoyt, D.G. , Stevens, R.L. , and Schneider, R.A. , Effects of synthetic disintegrins on breast cancer in mice. Era of Hope Proceedings, p.102. 2008.
DnaN clamp zones provide a platform for spatiotemporal coupling of mismatch detection to DNA replication

Mismatch repair (MMR) increases the fidelity of DNA replication by identifying and correcting replication errors. Processivity clamps are vital components of DNA replication and MMR, yet the mechanism and extent to which they participate in MMR remains unclear. We investigated the role of the Bacillus subtilis processivity clamp DnaN, and found that it serves as a platform for mismatch detection and coupling of repair to DNA replication. By visualizing functional MutS fluorescent fusions in vivo, we find that MutS forms foci independent of mismatch detection at sites of replication (i.e. the replisome). These MutS foci are directed to the replisome by DnaN clamp zones that aid mismatch detection by targeting the search to nascent DNA. Following mismatch detection, MutS disengages from the replisome, facilitating repair. We tested the functional importance of DnaN‐mediated mismatch detection for MMR, and found that it accounts for 90% of repair. This high dependence on DnaN can be bypassed by increasing MutS concentration within the cell, indicating a secondary mode of detection in vivo whereby MutS directly finds mismatches without associating with the replisome. Overall, our results provide new insight into the mechanism by which DnaN couples mismatch recognition to DNA replication in living cells.
Examination of Stability and Characteristics of Gyrator Type Bilateral Control; Toward Controller and Transfer Impedance Design

This paper examines a stability characteristic of gyrator type bilateral control in order to determine a definite feedback gain and scaling design. Stability is examined based on root locus. Maximum stable gain, transfer impedance and transfer admittance are observed in the examination. Moreover, it is observed that at certain transfer impedance and admittance, gyrator type bilateral control and transformer type bilateral control become equivalent to each other. Validity of the analysis and characteristics of several transfer impedance and transfer admittance design are examined based on experiments.
Controlling the privacy loss with the input feature maps of the layers in convolutional neural networks

We propose the method to sanitize the privacy of the IFM(Input Feature Map)s that are fed into the layers of CNN(Convolutional Neural Network)s. The method introduces the degree of the sanitization that makes the application using a CNN be able to control the privacy loss represented as the ratio of the probabilistic accuracies for original IFM and sanitized IFM. For the sanitization of an IFM, the sample-and-hold based approximation scheme is devised to satisfy an application-specific degree of the sanitization. The scheme approximates an IFM by replacing all the samples in a window with the non-zero sample closest to the mean of the sampling window. It also removes the dependency on CNN configuration by unfolding multi-dimensional IFM tensors into one-dimensional streams to be approximated.
A prospective cohort study of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus carriage in neonates: the role of maternal carriage and phenotypic and molecular characteristics

Background Staphylococcus aureus, particularly methicillin-resistant S. aureus (MRSA), in mothers can cause serious outcomes in neonates. We aimed to elucidate the associations of S. aureus and MRSA carriage between mothers and neonates. Methods A prospective cohort study was conducted between August and November 2015 in two hospitals in Shenzhen, China. Chinese pregnant women and their neonates who met the inclusion criteria were included in this study; samples and relevant information were collected. We assessed maternal–neonatal associations by using Poisson regression models. Results Overall, 1834 mothers and their neonates were included in this study. The prevalence of isolate carriage among the mothers was as follows: S. aureus (nasal, 25.8%; vaginal, 7.3%; and nasal and vaginal, 3.3%) and MRSA (nasal, 5.7%; vaginal, 1.7%; and nasal and vaginal, 0.5%). The incidences of S. aureus and MRSA carriage among neonates were 3.3% and 0.8%, respectively. Of the 21 maternal–neonatal pairs with S. aureus carriage, 14 were concordant pairs with the same phenotypic and molecular characteristics. After adjustment, the relative risks and 95% confidence intervals (CIs) between the S. aureus carriage of neonates and nasal S. aureus carriage, vaginal S. aureus carriage, and both nasal and vaginal S. aureus carriage of mothers were 2.8 (95% CI, 1.6–4.8), 7.1 (95% CI, 4.1–12.4), and 9.6 (95% CI, 4.2–22.4), respectively. Conclusion S. aureus carriage in mothers increases the risk for neonates.
Ordinal Depth Supervision for 3D Human Pose Estimation

Our ability to train end-to-end systems for 3D human pose estimation from single images is currently constrained by the limited availability of 3D annotations for natural images. Most datasets are captured using Motion Capture (MoCap) systems in a studio setting and it is difficult to reach the variability of 2D human pose datasets, like MPII or LSP. To alleviate the need for accurate 3D ground truth, we propose to use a weaker supervision signal provided by the ordinal depths of human joints. This information can be acquired by human annotators for a wide range of images and poses. We showcase the effectiveness and flexibility of training Convolutional Networks (ConvNets) with these ordinal relations in different settings, always achieving competitive performance with ConvNets trained with accurate 3D joint coordinates. Additionally, to demonstrate the potential of the approach, we augment the popular LSP and MPII datasets with ordinal depth annotations. This extension allows us to present quantitative and qualitative evaluation in non-studio conditions. Simultaneously, these ordinal annotations can be easily incorporated in the training procedure of typical ConvNets for 3D human pose. Through this inclusion we achieve new state-of-the-art performance for the relevant benchmarks and validate the effectiveness of ordinal depth supervision for 3D human pose.
Low-Rank Graph-Regularized Structured Sparse Regression for Identifying Genetic Biomarkers

In this paper, we propose a novel sparse regression method for Brain-Wide and Genome-Wide association study. Specifically, we impose a low-rank constraint on the weight coefficient matrix and then decompose it into two low-rank matrices, which find relationships in genetic features and in brain imaging features, respectively. We also introduce a sparse acyclic digraph with sparsity-inducing penalty to take further into account the correlations among the genetic variables, by which it can be possible to identify the representative SNPs that are highly associated with the brain imaging features. We optimize our objective function by jointly tackling low-rank regression and variable selection in a framework. In our method, the low-rank constraint allows us to conduct variable selection with the low-rank representations of the data; the learned low-sparsity weight coefficients allow discarding unimportant variables at the end. The experimental results on the Alzheimer’s Disease Neuroimaging Initiative (ADNI) dataset showed that the proposed method could select the important SNPs to more accurately estimate the brain imaging features than the state-of-the-art methods.
Human CHCHD 4 mitochondrial proteins regulate cellular oxygen consumption rate and metabolism and provide a critical role in hypoxia signaling and tumor progression

Increased expression of the regulatory subunit of HIFs (HIF-1a or HIF-2a) is associated with metabolic adaptation, angiogenesis, and tumor progression. Understanding how HIFs are regulated is of intense interest. Intriguingly, the molecular mechanisms that link mitochondrial function with the HIF-regulated response to hypoxia remain to be unraveled. Here we describe what we believe to be novel functions of the human gene CHCHD4 in this context. We found that CHCHD4 encodes 2 alternatively spliced, differentially expressed isoforms (CHCHD4.1 and CHCHD4.2). CHCHD4.1 is identical to MIA40, the homolog of yeast Mia40, a key component of the mitochondrial disulfide relay system that regulates electron transfer to cytochrome c. Further analysis revealed that CHCHD4 proteins contain an evolutionarily conserved coiled-coil-helix-coiled-coil-helix (CHCH) domain important for mitochondrial localization. Modulation of CHCHD4 protein expression in tumor cells regulated cellular oxygen consumption rate and metabolism. Targeting CHCHD4 expression blocked HIF-1a induction and function in hypoxia and resulted in inhibition of tumor growth and angiogenesis in vivo. Overexpression of CHCHD4 proteins in tumor cells enhanced HIF-1a protein stabilization in hypoxic conditions, an effect insensitive to antioxidant treatment. In human cancers, increased CHCHD4 expression was found to correlate with the hypoxia gene expression signature, increasing tumor grade, and reduced patient survival. Thus, our study identifies a mitochondrial mechanism that is critical for regulating the hypoxic response in tumors. Research Article Oncology
A photovoltaic panel modelling method for flexible implementation in Matlab/Simulink using datasheet quantities

This paper presents a detailed method for creating an embedded Matlab model in Simulink for any solar photovoltaic panel starting with its datasheet values. It links extrinsic functions to the Simulink embedded model to provide fast and simple iterative solving of non-linear equations. It also provides a method sufficiently flexible to produce a model output based on panel current or voltage such that it can be cascaded with different Simulink elements.
The ER membrane protein complex is a transmembrane domain insertase

A new way into the ER Membrane-embedded proteins are highly diverse in topology, physical characteristics, and location. This diversity necessitates multiple pathways for their effective membrane insertion. Guna et al. found that a widely conserved protein complex is responsible for inserting a subset of membrane proteins into the endoplasmic reticulum (ER) membrane (see the Perspective by Fry and Clemons Jr.). This ER membrane protein complex (EMC) inserts transmembrane domains whose topology and hydrophobicity preclude effective recognition by other insertion factors. This finding helps explain why the loss of EMC causes ER stress and altered protein trafficking. Science, this issue p. 470; see also p. 390 An endoplasmic reticulum membrane protein complex functions as an insertase for transmembrane domains of low to moderate hydrophobicity. Insertion of proteins into membranes is an essential cellular process. The extensive biophysical and topological diversity of membrane proteins necessitates multiple insertion pathways that remain incompletely defined. Here we found that known membrane insertion pathways fail to effectively engage tail-anchored membrane proteins with moderately hydrophobic transmembrane domains. These proteins are instead shielded in the cytosol by calmodulin. Dynamic release from calmodulin allowed sampling of the endoplasmic reticulum (ER), where the conserved ER membrane protein complex (EMC) was shown to be essential for efficient insertion in vitro and in cells. Purified EMC in synthetic liposomes catalyzed the insertion of its substrates in a reconstituted system. Thus, EMC is a transmembrane domain insertase, a function that may explain its widely pleiotropic membrane-associated phenotypes across organisms.
Training Latin American primary care physicians in the WPA module on depression: results of a multicenter trial

Background. In order to improve care for people with depressive disorders and to reduce the increasing burden of depression, the American Regional Office of the World Health Organization has launched a major region-wide initiative. A central part of this effort was directed to the primary care system where the diagnosis and treatment of depression are deficient in many countries. This study evaluated the materials developed by the World Psychiatric Association in a training program on depression among primary care physicians by measuring changes in their knowledge, attitudes, and practice (KAP). Method. One hundred and seven physicians and 6174 patients from five Latin American countries participated in the trial. KAP were assessed 1 month before and 1 month following the training program. In addition, the presence of depressive symptoms was measured in patients who visited the clinic during a typical week at both times using the Zung Depression Scale and a DSM-IV/ICD-10 major depression checklist. Results. The program slightly improved knowledge about depression and modified some attitudes, but had limited impact on actual practice. There was no evidence that the diagnosis of depression was made more frequently, nor was there an improvement in psychopharmacological management. The post-training agreement between physician diagnosis and that based on patient self-report remained low. The physicians, however, seemed more confident in treating depressed patients after training, and referred fewer patients to psychiatrists. Conclusions. Traditional means of training primary care physicians in depression have little impact on clinical practice regardless of the quality of the teaching materials.
Perancangan Aplikasi Daring Bimbingan Tugas Akhir

Proses menulis skripsi atau tugas akhir adalah tahap akhir yang harus ditempuh oleh mahasiswa yang pada prosesnya dibimbing oleh satu atau dua orang dosen pembimbing. Dalam prakteknya, aktifitas bimbingan tugas akhir ini dilakukan dengan diskusi, tanya jawab, pemberian masukan dan aktifitas lainnya. Penelitian ini mengusulkan perancangan aplikasi daring bimbingan tugas akhir yang diperuntukkan bagi mahasiswa dan dosen. Aplikasi ini dirancang dalam lingkup Program Studi Teknik Informatika Universitas Tanjungpura berdasarkan informasi dan prosedur pembuatan tugas akhir Program Studi Teknik Informatika. Adapun pengujian yang digunakan adalah metode black box dengan menggunakan teknik sample testing dan metode UAT ( User Acceptance Test ) untuk memeriksa apakah sistem dapat berjalan dengan benar sesuai dengan yang diharapkan oleh pengguna. Hasil dari penelitian ini memberikan kemudahan bagi kedua dosen pembimbing untuk berkomunikasi mengenai mahasiswa bimbingannya dalam forum pembimbing karena proses bimbingan dapat dilakukan secara daring serta dilengkapi dengan pemberitahuan secara realtime melalui pesan elektronik atau email . Kata kunci— tugas akhir, daring, bimbingan