Miracle-dz/UniFAR-FTUs huggingface dataset:
Early impairment of coronary flow reserve is not associated with Chlamydia pneumoniae antibodies

BACKGROUND: Chlamydia pneumoniae infection has been associated with atherosclerosis by sero-epidemiological, histopathological and interventional studies, and animal experiments. We hypothesized that if chlamydial infection is causative of atherosclerosis, the occurrence of antibodies against C. pneumoniae should be associated with coronary vasomotor dysfunction - an early sign of atherosclerosis. AIM: To study the association between C. pneumoniae infection and coronary vasomotor function in young men without signs of ischemic heart disease. METHODS: Serum IgG and IgA antibody concentrations against C. pneumoniae were determined in 125 clinically healthy subjects undergoing positron emission tomography (PET) studies. Myocardial blood flow was measured at rest and during pharmacologically induced hyperemia using [ 15 O]H 2 O. Coronary flow reserve was calculated as the ratio of hyperemic blood flow to resting blood flow. RESULTS: No association was found between serum C. pneumoniae antibody concentrations and myocardial blood flow parameters. In contrast, more conventional risk factors for coronary artery disease, such as total cholesterol and apolipoprotein B, were inversely associated with hyperemic flow and flow reserve. CONCLUSIONS: We found no association between C. pneumoniae antibodies and coronary vasomotor function in subjects without ischemic heart disease. Thus, these results do not support the role of C. pneumoniae infection as an early phase risk factor for coronary artery disease.
Linksbetweenmethane £ uxand transcriptional activitiesof methanogensandmethaneoxidizers ina blanket peat bog

The relationship between biogeochemical process rates and microbial functional activity was investigated by analysis of the transcriptional dynamics of the key functional genes for methanogenesis (methyl coenzyme M reductase; mcrA) and methane oxidation (particulate methane monooxygenase; pmoA) and in situ methane flux at two peat soil field sites with contrasting net methane-emitting and -oxidizing characteristics. qPCR was used to quantify the abundances of mcrA and pmoA genes and transcripts at two soil depths. Total methanogen and methanotroph transcriptional dynamics, calculated from mcrA and pmoA gene : transcript abundance ratios, were similar at both sites and depths. However, a linear relationship was demonstrated between surface mcrA and pmoA transcript dynamics and surface flux rates at the methane-emitting and methane-oxidizing sites, respectively. Results indicate that methanotroph activity was at least partially substrate-limited at the methane-emitting site and by other factors at the methaneoxidizing site. Soil depth also contributed to the control of surface methane fluxes, but to a lesser extent. Small differences in the soil water content may have contributed to differences in methanogen and methanotroph activities. This study therefore provides a first insight into the regulation of in situ, field-level surface CH4 flux at the molecular level by an accurate reflection of gene : transcript abundance ratios for the key genes in methane generation and consumption.
Caffeine Deprivation State Modulates Expression of Acquired Liking for Caffeine-Paired Flavours

Previous studies found that caffeine consumers acquired a liking for the flavour of novel caffeinated drinks when these drinks were consumed repeatedly in a caffeine-deprived, but not nondeprived, state. Expression of this acquired liking appeared acutely sensitive to current caffeine deprivation state, but the use of between-subjects designs confounded interpretation of those studies. The present study evaluated these findings further using a within-subject design, with one flavour paired with caffeine (CS +) and the second with the absence of caffeine (CS–). During four CS + and four CS– training days, 32 moderate caffeine consumers alternatively consumed a novel flavoured drink a CS + paired with caffeine and a CS– flavour paired with placebo. Participants evaluated both drinks before and after training in two motivational states: caffeine deprived and nondeprived. As predicted, pleasantness ratings for the caffeine-paired flavour increased overall. However, this acquired liking was only significant when tested in a caffeine-deprived state. These data are consistent with a conditioned-flavour preference model and imply that expression of acquired liking for a novel caffeinated flavour depends on the need for the effects of caffeine at the time when the drink is evaluated.
The Wavelength Sensor Using Optical Directional Coupler

This paper proposes a wavelength sensor based on the optical directional coupler. The finite-difference time-domain (FDTD) is used to calculate of the light intensity propagating through the structure, and to find the appropriate dimensions of the structure. The structure with the size of 0.4 mum times 0.4 mum, and particular lengths would provide the coupling efficiency varying linearly with wavelengths. Thus, the structure could be employed as the wavelength sensor for the wavelength in a range between 1.2 mum and 1.6 mum
Cafe Variome: General‐Purpose Software for Making Genotype–Phenotype Data Discoverable in Restricted or Open Access Contexts

Biomedical data sharing is desirable, but problematic. Data “discovery” approaches—which establish the existence rather than the substance of data—precisely connect data owners with data seekers, and thereby promote data sharing. Cafe Variome (http://www.cafevariome.org) was therefore designed to provide a general‐purpose, Web‐based, data discovery tool that can be quickly installed by any genotype–phenotype data owner, or network of data owners, to make safe or sensitive content appropriately discoverable. Data fields or content of any type can be accommodated, from simple ID and label fields through to extensive genotype and phenotype details based on ontologies. The system provides a “shop window” in front of data, with main interfaces being a simple search box and a powerful “query‐builder” that enable very elaborate queries to be formulated. After a successful search, counts of records are reported grouped by “openAccess” (data may be directly accessed), “linkedAccess” (a source link is provided), and “restrictedAccess” (facilitated data requests and subsequent provision of approved records). An administrator interface provides a wide range of options for system configuration, enabling highly customized single‐site or federated networks to be established. Current uses include rare disease data discovery, patient matchmaking, and a Beacon Web service.
Content-aware rate control scheme to improve the energy efficiency for mobile IPTV

In mobile IPTV service, energy consumption of devices is an important parameter that affects quality of experience (QoE) such as continuity of the service. However, existing research to provide network-aware rate control does not guarantee the QoE. This paper proposes a new streaming system that improves energy efficiency by reducing the transmission of unnecessary bitstreams. It utilizes the variance of PSNR and distortion characteristics of content on the basis of a network-aware streaming system using SVC.
Proactive Autonomous Resource Enrichment for E-Learning

Information mastering is the major use case for learners in e-learning systems. Therefore they need appropriate search and retrieval mechanisms. An approach to overcome potentially occuring problems, like e.g. high recall and low precision or the high result sensibility to the used vocabulary, is the presentation of preselected content. This paper presents an approach for the automatic ontology-based enrichment of e-learning content.
Functional Cross-talk between Fatty Acid Synthesis and Nonribosomal Peptide Synthesis in Quinoxaline Antibiotic-producing Streptomycetes*

Quinoxaline antibiotics are chromopeptide lactones embracing the two families of triostins and quinomycins, each having characteristic sulfur-containing cross-bridges. Interest in these compounds stems from their antineoplastic activities and their specific binding to DNA via bifunctional intercalation of the twin chromophores represented by quinoxaline-2-carboxylic acid (QA). Enzymatic analysis of triostin A-producing Streptomyces triostinicus and quinomycin A-producing Streptomyces echinatus revealed four nonribosomal peptide synthetase modules for the assembly of the quinoxalinoyl tetrapeptide backbone of the quinoxaline antibiotics. The modules were contained in three protein fractions, referred to as triostin synthetases (TrsII, III, and IV). TrsII is a 245-kDa bimodular nonribosomal peptide synthetase activating as thioesters for both serine and alanine, the first two amino acids of the quinoxalinoyl tetrapeptide chain. TrsIII, represented by a protein of 250 kDa, activates cysteine as a thioester. TrsIV, an unstable protein of apparent Mr about 280,000, was identified by its ability to activate and N-methylate valine, the last amino acid. QA, the chromophore, was shown to be recruited by a free-standing adenylation domain, TrsI, in conjunction with a QA-binding protein, AcpPSE. Cloning of the gene for the QA-binding protein revealed that it is the fatty acyl carrier protein, AcpPSE, of the fatty acid synthase of S. echinatus and S. triostinicus. Analysis of the acylation reaction of AcpPSE by TrsI along with other A-domains and the aroyl carrier protein AcmACP from actinomycin biosynthesis revealed a specific requirement for AcpPSE in the activation and also in the condensation of QA with serine in the initiation step of QA tetrapeptide assembly on TrsII. These data show for the first time a functional interaction between nonribosomal peptide synthesis and fatty acid synthesis.
New Insights into the Non-orthodox Two Component Rcs Phosphorelay System

The Rcs phosphorelay system, a non-orthodox two-component regulatory system, integrates environmental signals, regulates gene expression, and alters the physiological behavior of members of the Enterobacteriaceae family of Gram-negative bacteria. Recent studies of Rcs system focused on protein interactions, functions, and the evolution of Rcs system components and its auxiliary regulatory proteins. Herein we review the latest advances on the Rcs system proteins, and discuss the roles that the Rcs system plays in the environmental adaptation of various Enterobacteriaceae species.
A Novel Enhancer of the Apaf1 Apoptosome Involved in Cytochrome c-dependent Caspase Activation and Apoptosis*

Apaf1/CED4 family members play central roles in apoptosis regulation as activators of caspase family cell death proteases. These proteins contain a nucleotide-binding (NB) self-oligomerization domain and a caspase recruitment domain (CARD). A novel human protein was identified, NAC, that contains anNB domain and CARD. The CARD of NAC interacts selectively with the CARD domain of Apaf1, a caspase-activating protein that couples mitochondria-released cytochrome c (cyt-c) to activation of cytosolic caspases. Cyt-c-mediated activation of caspases in cytosolic extracts and in cells is enhanced by overexpressing NAC and inhibited by reducing NAC using antisense/DNAzymes. Furthermore, association of NAC with Apaf1 is cyt c-inducible, resulting in a mega-complex (>1 MDa) containing both NAC and Apaf1 and correlating with enhanced recruitment and proteolytic processing of pro-caspase-9. NAC also collaborates with Apaf1 in inducing caspase activation and apoptosis in intact cells, whereas fragments of NAC representing only the CARD or NB domain suppress Apaf1-dependent apoptosis induction. NAC expressionin vivo is associated with terminal differentiation of short lived cells in epithelia and some other tissues. The ability of NAC to enhance Apaf1-apoptosome function reveals a novel paradigm for apoptosis regulation.
Minimizing Onsite Organic Household Left-Over Waste: The Emission Benefits of Keeping Pet Rabbits.

As waste management is becoming all the more crucial, this study investigates the way in which house left-over organic waste can be better managed on site, in order to minimize the off-site treatment cost and maximize environmental performance. For the implementation of this research, a full year measurement was recorded, showing the organic leftover waste food intake of two rabbits in a household of four. The organic food, collected in two separate baskets suitable for composting—though one for rabbit intake—was 168.5 kg in total, plus 68.8 kg, which was delivered directly to the composting bin, along with food remains and rabbit feces. The results show that, over the examined year, a total of up to 0.417 metric tons of CO2 equivalent per year emissions was avoided, suggesting that if 30 houses were to apply this methodology, one garbage truck journey per year would be saved. Overall, this study suggests that better information and environmental awareness can result in on-site, low cost, individual management of recyclable organic material, which would assist with the decrease in the cost of management, along with increased environmental performance.
Oviposition preference and offspring performance in container breeding mosquitoes: evaluating the effects of organic compounds and laboratory colonisation

1. The preference–performance hypothesis (PPH) predicts that organisms lacking parental care should oviposit in habitats that optimise offspring performance. Preference–performance relationships were investigated for the Asian tiger mosquito (Aedes albopictus Skuse) and the southern house mosquito (Culex quinquefasciatus Say) (Diptera: Culicidae), two medically important container‐breeding species, in response to an organic chemical blend mimicking decaying plant matter. Additionally, the effects of long‐term laboratory colonisation of Cx. quinquefasciatus using wild and laboratory strains were evaluated.
Study time and overload in engineering education

The nationwide joint research project “ZEITLast” aims to analyze the workload of students. Within this project the Ilmenau University of Technology will conduct a case study in three engineering courses of study. The focus is to identify possible temporal and teaching-based factors of stress and find methods of rearranging teaching methods and lesson plans to decrease the study workload. Particularly, the project is characterized by its method of detailed temporal data.
Correlation and prediction of mass transport across membranes. I. Influence of alkyl chain length on flux-determining properties of barrier and diffusant.

Abstract The effect of alkyl chain length on the physicochemical properties of a series of p -aminobenzoate esters, determinant of the rate at which they penetrate inert barriers, was investigated. Relationships are drawn for the influence of chain length on partition coefficient and solubility; and these, in conjunction with a diffusion layer membrane model, have been incorporated into a general, theoretical expression for maximum steady-state flux as a function of homolog size. This expression predicts a parabolic dependency as the homologous series is ascended for certain reasonably normal conditions. This model was tested and found valid for an uninterrupted ester series containing from one to seven carbons, utilizing dimethylpolysiloxane membranes. An analysis of the effect of each parameter in the equation was performed with computer assistance. This analysis shows the extent to which the optimum chain length for absorption (barrier penetration) is dependent on controllable and uncontrollable experimental factors. The broad applications of the diffusion layer membrane model with superimposed solubility constraints for the interpretation of membrane transport phenomena, including bioavailability, are detailed.
Doxycycline directly targets PAR1 to suppress tumor progression

Doxycycline have been reported to exert anti-cancer activity and have been assessed as anti-cancer agents in clinical trials. However, the direct targets of doxycycline in cancer cells remain unclear. In this study, we used a chemical proteomics approach to identify the Protease-activated receptor 1 (PAR1) as a specific target of inhibition of doxycycline. Binding assays and single-molecule imaging assays were performed to confirm the inhibition of doxycycline to PAR1. The effect of doxycycline on multi-omics and cell functions were assessed based on a PAR1/thrombin model. Molecular docking and molecular dynamic simulations revealed that doxycycline interacts with key amino acids in PAR1. Mutation of PAR1 further confirmed the computation-based results. Moreover, doxycycline provides highly selective inhibition of PAR1 signaling in tumors in vitro and in vivo. Using pathological clinical samples co-stained for doxycycline and PAR1, it was found that doxycycline fluorescence intensity and PAR1 expression shown a clear positive correlation. Thus, doxycycline may be a useful targeted anti-cancer drug that should be further investigated in clinical trials.
Constraints Contributed by Chromatin Looping Limit Recombination Targeting during Ig Class Switch Recombination

Engagement of promoters with distal elements in long-range looping interactions has been implicated in regulation of Ig class switch recombination (CSR). The principles determining the spatial and regulatory relationships among Igh transcriptional elements remain poorly defined. We examined the chromosome conformation of C region (CH) loci that are targeted for CSR in a cytokine-dependent fashion in mature B lymphocytes. Germline transcription (GLT) of the γ1 and ε CH loci is controlled by two transcription factors, IL-4–inducible STAT6 and LPS-activated NF-κB. We showed that although STAT6 deficiency triggered loss of GLT, deletion of NF-κB p50 abolished both GLT and γ1 locus:enhancer looping. Thus, chromatin looping between CH loci and Igh enhancers is independent of GLT production and STAT6, whereas the establishment and maintenance of these chromatin contacts requires NF-κB p50. Comparative analysis of the endogenous γ1 locus and a knock-in heterologous promoter in mice identified the promoter per se as the interactive looping element and showed that transcription elongation is dispensable for promoter/enhancer interactions. Interposition of the LPS-responsive heterologous promoter between the LPS-inducible γ3 and γ2b loci altered GLT expression and essentially abolished direct IgG2b switching while maintaining a sequential μ→γ3→γ2b format. Our study provides evidence that promoter/enhancer looping interactions can introduce negative constraints on distal promoters and affect their ability to engage in germline transcription and determine CSR targeting.
Discrepancy Between the Dynamic Computed Tomography and T2* Magnetic Resonance Perfusion Imaging in Brain Tumors: A Report of 2 Cases

In this report, we separately performed dynamic computed tomographic perfusion and dynamic susceptibility contrast-T2* magnetic resonance perfusion imaging on 2 cases of brain tumors (one was a glioblastoma, and the other was a central neurocytoma). Between the 2 methods, we saw the discrepancy in values of cerebral blood volume and cerebral blood flow, differences in location of the maximal cerebral blood volume, and regions with abnormal increased cerebral blood flow besides the solid part of the tumors. Both differences and similarities of the 2 techniques with their advantages and pitfalls were analyzed in detail. The developing trends in the near future were also discussed.
One enzyme, many reactions: structural basis for the various reactions catalyzed by naphthalene 1,2-dioxygenase

Rieske nonheme iron oxygenases often catalyze different reactions when different substrates bind to them. From the structures of complexes of naphthalene 1,2-dioxygenase with substrates that result in different types of products, it is shown that the binding orientation of the substrate not only dictates regioselectivity and stereospecificity, but also determines the type of reaction that is catalyzed.
Peripheral blood mononuclear cells develop into multinucleated osteoclasts in tissue culture

Previous studies have shown that osteoclasts are derived from mononuclear cells of hemopoietic bone marrow and peripheral blood. The purpose of this study was to demonstrate the presence of multinucleated osteoclasts after adding mononuclear cells from peripheral blood into established explants of fetal mouse calvaria in vitro.
Extended-state-observer-based adaptive control for synchronisation of multi-agent systems with unknown nonlinearities

This paper studies the problem of synchronisation to a desired trajectory for non-linear multi-agent systems. By introducing extended state observer approach, decentralised adaptive controllers are designed for distributed systems which have non-identical unknown non-linear dynamics. The non-identical unknown non-linear dynamics allows for a tracked command dynamics which is also non-linear and unknown. State variables of agents can be obtained only in the case where leader agent and the network communication topology for multi-agent systems is strongly connected digraph network structures. A Lyapunov-function-based approach is given to show that the tracking error is ultimately bounded. Some simulation results are given to demonstrate the effectiveness of the developed techniques in this paper.
Hydrogeologic Controls on Induced Seismicity in Crystalline Basement Rocks Due to Fluid Injection into Basal Reservoirs

A series of Mb 3.8–5.5 induced seismic events in the midcontinent region, United States, resulted from injection of fluid either into a basal sedimentary reservoir with no underlying confining unit or directly into the underlying crystalline basement complex. The earthquakes probably occurred along faults that were likely critically stressed within the crystalline basement. These faults were located at a considerable distance (up to 10 km) from the injection wells and head increases at the hypocenters were likely relatively small (∼70–150 m). We present a suite of simulations that use a simple hydrogeologic‐geomechanical model to assess what hydrogeologic conditions promote or deter induced seismic events within the crystalline basement across the midcontinent. The presence of a confining unit beneath the injection reservoir horizon had the single largest effect in preventing induced seismicity within the underlying crystalline basement. For a crystalline basement having a permeability of 2 × 10−17 m2 and specific storage coefficient of 10−7/m, injection at a rate of 5455 m3/d into the basal aquifer with no underlying basal seal over 10 years resulted in probable brittle failure to depths of about 0.6 km below the injection reservoir. Including a permeable (kz = 10−13 m2) Precambrian normal fault, located 20 m from the injection well, increased the depth of the failure region below the reservoir to 3 km. For a large permeability contrast between a Precambrian thrust fault (10−12 m2) and the surrounding crystalline basement (10−18 m2), the failure region can extend laterally 10 km away from the injection well.