Miracle-dz/UniFAR-FTUs huggingface dataset:
ImmunoMax. A maximized immunohistochemical method for the retrieval and enhancement of hidden antigens.

BACKGROUND Since the introduction of mAb, immunohistochemistry has become an important tool in research and in surgical pathology. The most widely used fixative in routine histopathology is formaldehyde, and it has become the gold standard for morphologic tissue preservation. Although the molecular mechanism underlying the tissue fixation is not well understood, it has become clear that available immunoreactive Ag are progressively lost during the fixation process. For a long time, it was thought that formalin-sensitive Ag might be irreversibly destroyed during the fixation process. Although monoclonal anti-Ig Ab frequently worked inadequately, polyclonal anti-Ig Ab were shown to produce reproducible staining results. It thus appeared possible that most cellular Ag might not be irreversibly destroyed but only masked. EXPERIMENTAL DESIGN Although some Ag may be retrieved under appropriate conditions, there might still be many for which available antigenic epitopes are still too sparse to be visualized, as observed for a large number of leukocyte differentiation Ag. One reliable approach to resolve this dilemma is the use of a combination of an optimized Ag retrieval system and a powerful immunohistochemical staining protocol introducing a biotin amplification step, in which signal amplification is accomplished by covalent deposition of biotin molecules. RESULTS Cryostat and paraffin sections were stained with the avidin-biotin complex technique and, for comparison, with the new maximized immunohistochemical staining protocol, termed the ImmunoMax method. Each step was monitored to establish how effectively it enhanced the overall sensitivity. Although pretreatment with detergent, protease, a chaotropic substance, or microwave heating resulted in only moderately improved immunostaining, the biotinylated tyramine enhancement step proved to be the most efficient one, although the latter is not sufficient for many Ag when used without pretreatment steps. The combination of an Ag retrieval step with the biotinylated tyramine enhancement step resulted in a 100 to 10,000-fold boost in sensitivity without loss of specificity. CONCLUSIONS With the ImmunoMax method, defined Ag can be reproducibly detected in formalin-fixed, paraffin-embedded tissues, and the sensitivity of the method is tremendously enhanced. Moreover, it also allows many previously unreactive or unsatisfactorily reactive Ag to be detected, as shown here for IgD, IgM, and CD7 with the use of mAb.
Lost in a Universe of Markets: Toward a Theory of Market Scoping for Early-Stage Technologies

This article examines market scoping for early-stage technologies, a fundamental yet underexplored marketing activity. Market scoping refers to managerial activities directed at the identification of market spaces for early-stage technologies. This discovery-oriented research aimed at theory development draws on an extensive, multiyear database of email trails and archival records detailing market-scoping efforts for early-stage technologies emerging from a global research university. From this longitudinal database, the authors provide an in-depth examination of managers’ market space decisions and advance an initial theory of market scoping. They isolate managers’ market-scoping mindset—which manifests as market ambiguity avoidance or acceptance—as a key explanatory construct shaping market space decisions and outcomes. Market ambiguity avoidance results in managers’ downstream orientation toward end users; this mindset, counterintuitively, may lead to technology commercialization failure. In contrast, market ambiguity acceptance results in managers’ upstream orientation; this mindset directs attention away from end users but helps uncover indirect paths to viable market spaces. This article lays the groundwork for advancing marketing research in the context of early-stage technology commercialization.
Design and analysis of neodymium free SPOKE-type motor with segmented wing shape permanent-magnet for concentrating flux density

This paper proposes a new design of wing shaped SPOKE-type permanent magnet brush-less direct current (BLDC) motor using ferrite permanent magnet (Fe-PM) instead of neodymium permanent magnet (Nd-PM). Here, a method to maximize the torque density is developed. The proposed model has an improved output characteristic as it uses the properties of the magnetic flux effect of the SPOKE-type motor with an additional pushing assistant Fe-PM and sub assistant Fe-PM in the shape of SPOKE. This is optimized by using proposed analytical calculation method for improving torque density. This paper validates results of the analytical model by finite element analysis (FEA) for proposed wing shaped SPOKE-type BLDC motors.
Non-Toxic Effect of Monascus purpureus Extract on Lactic Acid Bacteria Suggested Their Application in Fermented Foods.

The effect of Monascus purpureus extract (MPE) on probiotic lactic acid bacteria (LAB) was investigated to ascertain its application in fermented foods. Viable count of LAB was not affected after 24 hours of incubation in Man Rogosa Sharpe (MRS) broth containing MPE. The agar well-diffusion assay did not show any inhibition zone. The biotransformation of isoflavone glycosides by LAB in culture medium supplemented with MPE increased antioxidant activities. These data suggest that, nutritive and biological functionality of fermented foods can be improved by the use of MPE.
Waveguide filters for satellites

This article describes the historical evolution, and its theoretical and technological features of waveguide filters. All-metal waveguide filters have been widely used in satellite payloads since the advent of the first space communication systems four decades ago. In this period, such technology has been continuously evolving due to the increasing requirements demanded to these components. Special attention has also been paid to present the most recent advances in the development of CAD tools for satellite filters, which are based on full-wave electromagnetic analysis methods. Finally, the compact solutions for the input and output stages of satellite payloads are focused on, thus considering the evanescent mode waveguide filter technology.
Why Exports Adjust: Missing Imported Inputs or Lack of Credit?

This paper examines the role of imported intermediate inputs and credit constraints on exports adjustment. For identification, we study an episode of capital controls on outflows that exogenously restricted firms’ ability to pay for imports and the large-scale credit crunch that followed the imposition of controls in Greece in June 2015. Exploiting within-firm variation across sectors, we find that lack of imported inputs explains the drop in exports at the intensive margin, while lack of long-term credit is associated with adjustments at the extensive margin. Multinationals overcome liquidity constraints because of access to parents’ internal funds, but not import constraints because of stronger linkages for specialized inputs abroad. Our findings point to a novel result: the importance of both channels – real and finance – in jointly determining trade adjustment, and the different implications for the margins of trade
Membrane filtration of dairy products for microbiological analysis

Incubation with protease or Tween 80 or both dramatically improved the membrane filterability of liquid milks, powdered skim milk, and frozen dairy products without reducing the viability of five common species of bacteria. The technique can permit isolation and enumeration of microorganisms from test samples of these foods as large as 5 g. Flow direction through the filter was an important factor in filterability of dairy products.
Contribution à la commande dynamique référencée capteurs de robots parallèles

Ces travaux de recherche concernent la prediction et l'amelioration de la precision des machines a structure parallele. Ces travaux s'interessent principalement aux machines utilisees pour des tâches complexes comme la manipulation rapide d'objets ou l'usinage a grande vitesse. Ces travaux s'interessent a la coherence du triptyque modelisation - identification - commande. L'originalite de ces travaux est la prise en compte au niveau de la commande, et au moyen d'une perception adaptee, des specificites de la machine et de la dynamique de la tâche. L'apport principal de ces travaux de these est de proposer une commande dynamique dans l'espace Cartesien tout en utilisant une mesure exteroceptive de la pose de l'effecteur. Cette strategie de commande permet notamment une precision plus importante que les strategies classiques. En effet, le nombre d'estimations utilisees est plus faible, principalement au niveau de l'estimation du comportement dynamique. De plus, la regulation dans l'espace Cartesien permet la maitrise complete du mouvement de l'effecteur, notamment au niveau des singularites de la machine. Ces ameliorations sont validees en simulation. Le developpement d'un capteur visuel rapide a 1 kHz a egalement permis une validation experimentale de ces travaux.
Safety and efficacy of ketamine infusion in late onset depression, and conversion to treatment response

Byline: Shrikant. Srivastava, Radhey. Gangwar, Ambrish. Kumar Sir, Response rates to first line antidepressant therapy are abysmally low, and more so in older adults. Ketamine has been used successfully in treatment-resistant depression (TRD), and in nongeriatric population, with response rates at 24 and 72 h postinfusion being 25−85% and 14−72%, respectively. [sup][1] The postulated mechanism of action is through antagonism of N-methyl-D-aspartate receptors. [sup][2] A 65-year-old retired civil servant, living with her husband had fourth episode of depressive illness. In the last 3 years, the illness showed increasing resistance to antidepressant medication(s) with each additional episode. The first episode remitted with escitalopram (10 mg/day), the second episode required higher dosage of escitalopram (40 mg/day), and later, augmentation with amisulpride 25 mg/day, and the third episode remitted with duloxetine (up to 60 mg/day) (as she had relapsed while on escitalopram) and amisulpride 100 mg/day. In the Inter-episode period, the patient had attended all follow-up appointments and continued with prescribed medication. In this episode, she had failed treatment with duloxetine + amisulpride, agomelatine, and agomelatine + amisulpride, respectively. Informed consent was obtained from the patient. Ketamine infusion (0.5 mg/kg diluted in 100 ml of normal saline) was given over 40 min, under the supervision of consultant anesthetist (RSG). Ratings of mood (Hamilton rating scale for depression [HAMD])[sup][3] and side effects (Clinician Administered Dissociative States Scale)[sup][4] were undertaken on morning of the infusion, and 1, 2, and 4 h following infusions, and on in between days of the infusion [labelled as postinfusion days in Table 1]. Four days after the 4[sup]th infusion (HAMD = 10), the patient feeling very much improved subjectively, the ketamine infusions were discontinued, with the provision that further infusions may be required if the depressive symptoms recurred. Agomelatine 50 mg/day was continued through the infusion period and in the follow-up phase. {Table 1} Remission (HAMD The side effects reported with the infusions were − alteration of passage of time (infusion 3, hours 1 and 2) and mild gaps in memory (infusion 4, hour 2). …
Restriction of porcine parvovirus replication in nonpermissive cells

Swine testicle (ST) cells and Madin-Darby canine kidney (MDCK) cells differ in their ability to support replication of porcine parvovirus (PPV). Viral replication events in ST cells, a permissive cell type, and MDCK cells, a nonpermissive cell type, were compared in an attempt to elucidate putative mechanisms of restrictive virus replication. Radiolabeled PPV bound to the cell surface of both cell types equally well and the binding was shown to be PPV specific, indicating that the restriction was not at the cell surface level. In contrast, profound differences in intracellular events in PPV replication were observed between these two cell types. Synthesis of viral DNA was limited in MDCK cells in that the percentage of cells with replicative-form DNA as determined by strand-specific probe in situ hybridization was approximately 100-fold lower in MDCK cells than in ST cells at the same multiplicity of infection. Northern (RNA) blot analysis, using oligonucleotide probes derived from both structural and nonstructural protein-coding regions of the PPV genome, revealed four PPV mRNA transcripts from infected ST cells. Comparatively, RNA species from the structural protein coding region were actively transcribed in MDCK cells, but synthesis of RNA species from the nonstructural protein coding region was negligible. Immunoprecipitation of viral polypeptides revealed the three characteristic structural polypeptides, VP1, VP2, and VP3, along with the nonstructural polypeptide, NS-1 from ST cells. In contrast, neither viral structural or nonstructural polypeptides nor progeny virions were produced from MDCK cells. The data suggest that mechanisms controlling permissiveness of cells to PPV infection are associated with the level of viral DNA replication, RNA transcription, and viral antigen expression but not absorption to the cell surface.
Distributed Consensus of Multi-Agent Systems With Input Constraints: A Model Predictive Control Approach

The discrete-time double-integrator consensus problem is addressed for multi-agent systems with directed switching proximity topologies and input constraints. Some model predictive control protocols are developed to achieve stable consensus under the condition that the proximity graph has a directed spanning tree and the sampling period is sufficiently small. Moreover, the control horizon is extended to larger than one, which endows sufficient degrees of freedom to accelerate the convergence to consensus. Numerical simulations are conducted to show the effectiveness of the control algorithm.
The Expansion of a Tool to Measure Staff Involvement in and Attitudes Toward the Implementation of a Clinical Information System

The Clinical Information System Questionnaire (CISQ-15) is a new 15-item tool designed to measure staff involvement in and attitudes towards CIS implementations. It was developed during a clinical trial which tested the effects of a combined managerial and IT intervention on staff attitudes and patient outcomes. The CISQ-15 appears to have high construct validity and internal consistency, although further studies are needed. Such studies are under way, and an expanded 36-item version, the CISQ-36, is now being evaluated.
Human behavior recognition method based on conditional neural field

In this paper, a human action behavior method is proposed to identify the behavior of a single person on a public data set. After comparing different kinds of feature extraction algorithms, a robust adaptive visual background extraction algorithm is utilized to extract the algorithm feature. Then the centroid is used to intercept the target region and converted into a one-dimensional vector. Finally, we take advantage of feature vector for experiment training and testing. Comparing experimental result with that results of latent-dynamic conditional neural field model and support vector machine. The experimental result show that the conditional neural field model has higher recognition rate and better stability.
A SWOT Analysis of Landfill Management System: Case Study of Talangagung Edu-tourism Landfill in Malang, East Java

The educational tourism landfill management system was one way that could be applied by the landfill manager to support environment based waste management program and environmental conservation education. This study aimed to determine the status quo of Talangagung educational tourisme (edu-tourism) landfill, at Kepanjen, Malang as one landfill model of national pilot that had implemented controlled landfill management system in Indonesia. This research used qualitative approach by using SWOT and IFAS-EFAS analysis. SWOT and IFAS-EFAS analysis used to evaluate internal (strengths, weaknesses) and external (opportunities and threats) factors regarding landfill management policy as an edu-tourism landfll. Talangagung landfill hasd strength and opportunities which could be utilized for further policy development. Currently, the Talangagung landfill position was in growth quadrant based on SWOT and IFAS-EFAS analysis. The appropriate management policy strategy for the current Talangagung landfill condition was to utilizing and synergizing maximally the strengths and opportunities available to achieve better management. The policy could be selected to support further development of landfill was to strengthen social relationships with surrounding communities and visitors. Key wordsedu-tourism, landfill, waste management, SWOT
Relationship between laboratory-measured variables and heart rate during an ultra-endurance triathlon

The aim of the present study was to examine the relationship between the performance heart rate during an ultra-endurance triathlon and the heart rate corresponding to several demarcation points measured during laboratory-based progressive cycle ergometry and treadmill running. Less than one month before an ultra-endurance triathlon, 21 well-trained ultra-endurance triathletes (mean ± s: age 35 ± 6 years, height 1.77 ± 0.05 m, mass 74.0 ± 6.9 kg, = 4.75 ± 0.42 l · min−1) performed progressive exercise tests of cycle ergometry and treadmill running for the determination of peak oxygen uptake ( ), heart rate corresponding to the first and second ventilatory thresholds, as well as the heart rate deflection point. Portable telemetry units recorded heart rate at 60 s increments throughout the ultra-endurance triathlon. Heart rate during the cycle and run phases of the ultra-endurance triathlon (148 ± 9 and 143 ± 13 beats · min−1 respectively) were significantly (P < 0.05) less than the second ventilatory thresholds (160 ± 13 and 165 ± 14 beats · min−1 respectively) and heart rate deflection points (170 ± 13 and 179 ± 9 beats · min−1 respectively). However, mean heart rate during the cycle and run phases of the ultra-endurance triathlon were significantly related to (r = 0.76 and 0.66; P < 0.01), and not significantly different from, the first ventilatory thresholds (146 ± 12 and 148 ± 15 beats · min−1 respectively). Furthermore, the difference between heart rate during the cycle phase of the ultra-endurance triathlon and heart rate at the first ventilatory threshold was related to marathon run time (r = 0.61; P < 0.01) and overall ultra-endurance triathlon time (r = 0.45; P < 0.05). The results suggest that triathletes perform the cycle and run phases of the ultra-endurance triathlon at an exercise intensity near their first ventilatory threshold.
Development of an Innovative System for the Reconstruction of New Generation Satellite Images. (Développement de méthodes innovantes pour la restauration des images de télédétection spatiale de nouvelles générations)

Remote sensing satellites have demonstrated to be a helpful instrument. Indeed, satellite images have been successfully exploited to deal with several applications including environmental monitoring and prevention of natural disasters. In the last years, the increasing of the availability of very high spatial resolution (VHR) remote sensing images resulted in new potentially relevant applications related to land cover control and environmental management. In particular, optical sensors, due to the fact that they acquire directly the reflected light from the sun, they may suffer from the presence of clouds in the sky and/or of shadows on the earth. This involves the problem of missing data, which may results an important and crucial problem especially in the case of VHR images, where their higher geometrical details induce to bigger lost of information. In this thesis, new methodologies of detection and reconstruction of missing data region in VHR images are proposed and applied on areas contaminated by the presence of clouds and/or shadows. In particular, the proposed methodological contributions include: i) a multiresolution inpainting strategy to reconstruct cloud-contaminated images; ii) a new combination of radiometric information and spatial position information in two specific kernels to perform a better reconstruction of cloud-contaminated regions by adopting a support vector regression (SVR) method; iii) the exploitation of compressive sensing theory adopting three different strategies (orthogonal matching pursuit, basis pursuit and a genetic algorithm solution) for the reconstruction of cloud-contaminated images; iv) a complete processing chain which exploits a support vector machine (SVM) classification for the detection and a linear regression for the reconstruction of specific shadow areas; and v) several evaluation criteria capable to assess the reconstructability of shadow areas. All of them are specifically developed to work with VHR images. Experimental results conducted on real data are reported in order to show and confirm the validity of all the proposed methods. They all suggest that, despite the complexity of the problems, it is possible to recover in a good way missing areas obscured by clouds or shadows.
Building Teams from a Distance

Excerpt] Virtual teams are comprised of individuals that are separated geographically or organizationally and that rely primarily on technology to complete tasks (Powell, Piccoli & Ives, 2004). This work arrangement has been found to be advantageous for many firms because it reduces the costs and time associated with employee travel. It also permits organizations to attract and retain top talent because workplace flexibility is increasingly seen as a crucial aspect of job satisfaction for many employees (Bergiel, Bergiel & Balsmeier, 2008). Virtual teams are also valuable to many businesses because team members commonly focus their interests on tasks instead of shared social or cultural environments, which often impact the dynamic within conventional teams (Hamilton & Scandura, 2003). This fosters a working environment that encourages innovation and decreases discrimination by hierarchy, employee impairments, race or age because productivity is more important than other characteristics (Bergiel et al., 2008). While virtual teams have many advantages, they frequently struggle to establish a strong sense of trust between individuals, frequent team member intercommunication, and effective leadership; all of which are necessary for team success.
Research on Post-Adoption Behavior of B2B E-Marketplace in China

this paper examines post-adoption behavior (continue to use or not) within the context of B2B e-marketplace in China. first, based on innovation diffusion theory and relative research, ten key factors effecting organizations to continue to use B2B e-marketplace are identified, the theoretic model is built based on TOE framework and 10 hypotheses are formulated. Then, the model and ten hypothesis are tested using data collected from 176 Chinese enterprises. The result indicates that relative advantage, compatibility, network externality, top manager support, government E-readiness, market force E-readiness and support industries E-readiness have positive effects on organization's intention to continue to use B2B e-marketplace in China.