Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of walking recommendations in previously inactive women.

PURPOSE To compare the number of steps accumulated by women instructed to walk 10,000 steps per day (10K group) with those told to take a brisk 30-min walk on most, preferably all, days of the week (30-min group). METHODS Daily steps were compared for 58 sedentary women (mean age 45.0+/-6.0 yr) randomly assigned to either the 10K or the 30-min group. Subjects wore a sealed pedometer for 2 wk for baseline physical activity assessment. Those averaging <or=7000 steps per day were randomly assigned to a group for the 4-wk intervention. All subjects wore a sealed pedometer capable of storing 7 d of data, and reported to the laboratory each week so that investigators could gather step counts. The 10K group wore a second pedometer for viewing their daily steps. RESULTS There were no differences (P>0.05) between the groups for baseline steps. During the intervention, there was a significant difference (P<0.005) between groups in daily steps. The 30-min group walked 8270+/-354 steps per day, and the 10K group walked 10,159+/-292 steps per day. The 30-min group averaged 9505+/-326 steps per day on the days that a 30-min walk occurred, and 5597+/-363 steps per day when no walk occurred (P<0.05). The 10K group averaged 11,775+/-207 steps on days when they walked at least 10,000 steps, and 7780+/-231 steps on days that their target was not achieved (P<0.05). CONCLUSION Women walk more when told to take 10,000 steps per day compared with those instructed to take a brisk 30-min walk. On days when women took a 30-min walk, their average step count was near 10,000.
The Interacting UL31 and UL34 Gene Products of Pseudorabies Virus Are Involved in Egress from the Host-Cell Nucleus and Represent Components of Primary Enveloped but Not Mature Virions

ABSTRACT A 2.6-kbp fragment of the pseudorabies virus (PrV) genome was sequenced and shown to contain the homologues of the highly conserved herpesvirus genes UL31 and UL32. By use of a monospecific antiserum, the UL31 gene product was identified as a nuclear protein with an apparent molecular mass of 29 kDa. For functional analysis, UL31 was deleted by mutagenesis in Escherichia coli of an infectious full-length clone of the PrV genome. The resulting virus mutants were deficient in plaque formation, and titers were reduced more than 100-fold from those of wild-type PrV. Ultrastructural analyses demonstrated that capsid maturation and DNA packaging were not affected. However, neither budding at the inner nuclear membrane nor cytoplasmic or extracellular virus particles were observed. These replication defects were similar to those of a UL34 deletion mutant (B. G. Klupp, H. Granzow, and T. C. Mettenleiter, J. Virol. 74:10063–10073, 2000) and could be completely repaired in a cell line which constitutively expresses the UL31 protein. Yeast two-hybrid studies revealed that a UL31 fusion protein specifically interacts with plasmids of a PrV genome library expressing the N-terminal part of UL34. Vice versa, UL34 selected UL31-encoding plasmids from the library. Immunofluorescence studies and immune electron microscopy demonstrated that in cells infected with wild-type PrV, both proteins accumulate at the nuclear membrane, whereas in the absence of UL34 the UL31 protein is dispersed throughout the nucleus. Like the UL34 protein, the UL31 gene product is a component of enveloped virus particles within the perinuclear space and absent from mature virions. Our findings suggest that physical interaction between these two virus proteins might be a prerequisite for primary envelopment of PrV at the inner nuclear membrane and that this envelope is removed by fusion with the outer nuclear membrane.
A multiport theory of communications

Electromagnetics provides the ground for a physical theory of communications, while information theory and signal theory approach the problem from a purely mathematical point of view. Nevertheless, the latter theories frequently do refer to physical terms, such as: energy, power, noise, antennas, or waves. It is strange enough, that, at present time, there is no provision being made such that the usage of such terms in information theory is at least consistent with the governing physics. More of- ten than not, this results in less than optimum signal processing solutions and does not contribute to a complete understanding of communication systems. Circuit theoretic channel models can help to bridge the gap between the physics of electromagnetics, and the mathematical world of information theory. The multiport concept makes sure that important physical concepts like energy, power, or noise are captured correctly, and terms such as antennas or waves are applied consistently with their physical meanings in information theory and signal processing. We suggest how to make circuit theoretic channel models and apply them to wireless communication systems which uses multiple antennas at both ends of the link. We thereby show that, in contrast to common belief, arrays of closely spaced antennas actually do support the bandwidth- and power-efficient multi-stream transmission.
Biofilm formation by Paracoccus denitrificans requires a type I secretion system‐dependent adhesin BapA

Abstract Paracoccus denitrificans is a non‐swimming Gram‐negative bacterium, with versatile respiration capability which has remarkable potentials for bioremediation, especially in water treatment. Although biofilms are important in water treatment systems, the genetic mechanisms underlying the cellular adherence and biofilm formation of this bacterium remain unknown. We show that P. denitrificans forms a thin biofilm on surfaces at the air‐liquid interface under static conditions. The initial step of biofilm formation requires a biofilm‐associated protein BapA, which we identified by transposon mutant screening. BapA contains a unique sequence of dipeptide repeats of aspartate and alanine. Our data indicate that BapA is translocated to the extracellular milieu by a type 1 secretion system, where it enables the cells to attach to the substratum. Furthermore, superresolution microscopy shows that BapA is localized on the cell surface, which alters the cell surface hydrophobicity. Our results show a crucial role of BapA that promotes the adhesion and biofilm formation of P. denitrificans.
Apo raver1 structure reveals distinct RRM domain orientations

Raver1 is a multifunctional protein that modulates both alternative splicing and focal adhesion assembly by binding to the nucleoplasmic splicing repressor polypyrimidine tract protein (PTB) or to the cytoskeletal proteins vinculin and α‐actinin. The amino‐terminal region of raver1 has three RNA recognition motif (RRM1, RRM2, and RRM3) domains, and RRM1 interacts with the vinculin tail (Vt) domain and vinculin mRNA. We previously determined the crystal structure of the raver1 RRM1–3 domains in complex with Vt at 2.75 Å resolution. Here, we report crystal structure of the unbound raver1 RRM1–3 domains at 2 Å resolution. The apo structure reveals that a bound sulfate ion disrupts an electrostatic interaction between the RRM1 and RRM2 domains, triggering a large relative domain movement of over 30°. Superposition with other RNA‐bound RRM structures places the sulfate ion near the superposed RNA phosphate group suggesting that this is the raver1 RNA binding site. While several single and some tandem RRM domain structures have been described, to the best of our knowledge, this is the second report of a three‐tandem RRM domain structure.
Endoscopic treatment of upper GI hemorrhage with a novel irrigating hood attached to the endoscope.

BACKGROUND Despite advances in endoscopic treatment methods for upper GI hemorrhage, hemostasis is often difficult to achieve, particularly when the endoscopic view at the site of hemorrhage is poor because of the presence of mucus and blood. The investigators developed an end hood that facilitates endoscopic hemostatic procedures while simultaneously allowing irrigation of the bleeding site. The usefulness of this end hood for treatment of upper GI hemorrhage, excluding hemorrhage from varices, was evaluated. METHODS The end-hood piece was fabricated by drilling a side hole in the cap portion of a conventional transparent hood. An irrigation tube then was glued to the exterior surface of the hole. The fabricated transparent hood was placed on the tip of an endoscope. With the hood piece in place, hemoclip placement and other endoscopic hemostatic procedures were performed in 15 patients with nonvariceal upper GI hemorrhage. RESULTS Hemostasis was successfully achieved in all cases. In all cases of active hemorrhage, hemostatic treatment was enhanced by simultaneous irrigation beneath the hood. The median time required for the hemostatic procedure with the attached hood was 4.8 minutes. CONCLUSION The end-hood irrigation device and technique facilitate endoscopic hemostatic treatment of nonvariceal upper GI hemorrhage.
Autoradiographic quantification of 18F-FDG uptake in experimental soft-tissue abscesses in rats.

PURPOSE To use semiquantitative autoradiography to investigate fluorodeoxyglucose (FDG) uptake, distribution, and cellular localization in acute, early chronic, and late chronic soft-tissue infections. MATERIALS AND METHODS Unilateral calf-muscle abscesses were induced in 12 Sprague-Dawley rats by means of intramuscular inoculation of 0.1 mL of bacterial suspension (Staphylococcus aureus, 1.2 x 10(9) CFU/mL). Following injection of 130-180 MBq of fluorine 18 FDG, autoradiography of the abscess and contralateral muscle was performed (10-microm section thickness) on days 2, 5, and 9 after infection. Detailed spatial correlation of autoradiographs and histopathologic samples was performed by means of image fusion. Regions of interest were placed in the abscess wall, and measured gray values were converted to kilobecquerels per cubic centimeter according to kilobecquerels of injected activity per gram of body weight, which yielded standardized uptake values (SUVs). RESULTS Acute abscess formation was characterized by central necrosis predominantly surrounded by neutrophils and a second necrotic tissue layer that bordered neutrophil infiltrates peripherally. Areas with increased FDG uptake corresponded to cellular inflammatory infiltrates, mainly granulocytes. The corresponding SUV was calculated to be 4.08 +/- 0.65 (mean +/- SD). Early chronic phase showed mixed cellular infiltrate of granulocytes and macrophages that surrounded central necrosis with interspersed fibroblasts and only residual muscle necrosis layer within the abscess wall. FDG uptake was located where granulocytes and macrophages were present, as in acute infection (SUV = 5.32 +/- 2.30). Late chronic infection was characterized by a prominent layer of macrophages around residual central necrosis and fibroblast-enriched granulation tissue delineating the infection from muscle tissue. FDG uptake clearly coincided with the macrophages, and no substantial increase of FDG uptake was detected within fibroblast-enriched granulation tissue. The SUV was calculated as 7.97 +/- 0.21. Results of Kruskal-Wallis ANOVA demonstrated that the change in SUV with time was statistically significant (chi(2) = 7.42, P <. 05). CONCLUSION The highest FDG uptake coincides with areas of inflammatory cell infiltrates, predominantly in neutrophils in the acute phase and in macrophages in the chronic phase of soft-tissue infection.
Absence of blood pressure, metabolic, and inflammatory marker changes after adenotonsillectomy for sleep apnea in Greek children

Pediatric studies revealed associations of obstructive sleep‐disordered breathing (SDB) with inflammation, metabolic dysfunction, and elevated blood pressure. Evidence about effects of adenotonsillectomy on these abnormalities is scarce. Aim of this investigation was to assess changes in C‐reactive protein (CRP), circulating intercellular adhesion molecule‐1 (cICAM‐1), insulin and blood pressure levels after adenotonsillectomy for SDB in Greek children.
Association between plasma proprotein convertase subtisilin/kexin type 9 concentration and coronary artery calcification

Background Plasma proprotein convertase subtilisin/kexin type 9 (PCSK9) has been reported to be related to several risk factors and diseases such as inflammatory markers and coronary artery disease. The aim of present study was to investigate whether plasma PCSK9 concentration was associated with coronary artery calcification. Methods A total of 403 consecutive untreated patients with angina-like chest pain, who received electron beam computed tomography, were enrolled and a coronary artery calcification score (CACS) was also measured. The baseline clinical characteristics were collected and blood sample was taken after 12-h fasting. The plasma PCSK9 concentrations were determined by ELISA in all patients, and the relationship between plasma PCSK9 concentrations and CACS was investigated. Results Patients with coronary artery calcification (CACS > 0) had significant higher plasma PCSK9 concentrations compared with those (CACS = 0) without coronary artery calcification (258.58 ± 69.53 ng/mL vs. 202.53 ± 52.17 ng/mL, P < 0.001). Patients with highest PCSK9 concentrations had the highest CACS. Multivariable linear regression analysis suggested that PCSK9 was independently associated with coronary artery calcification (P = 0.002) after adjusting for traditional cardiovascular risk factors. Furthermore, the area under the curve for the plasma PCSK9 concentration in predicting coronary artery calcification was 0.736 (95% CI: 0.687–0.785, P < 0.001), with a sensitivity of 66% and specificity of 70%. Conclusion A positive association between plasma PCSK9 concentration and coronary artery calcification in untreated patients with angina-like chest pain was observed in our study, suggesting that further investigation may be needed in order to confirm our primary findings and explore the clinical implications.
Intravenous and oral l-alpha-acetylmethadol: pharmacodynamics and pharmacokinetics in humans.

Levo-alpha-acetylmethadol (LAAM) is a long-acting opioid agonist approved for use as a maintenance treatment for opioid dependence. Previous clinical studies report that the onset of the effects of LAAM is slower after parenteral administration than oral administration; however, preclinical studies suggest otherwise. This study examined the pharmacodynamic and pharmacokinetic profile of LAAM when given orally and intravenously to humans. Opioid-experienced volunteers (n = 6), who were not physically dependent on opioids, received LAAM (20 and 40 mg/70 kg i.v. and p.o.) and placebo under double-blind, double-dummy conditions during five weekly experimental sessions. Behavioral, physiological, subjective and pharmacokinetic measures were collected before and for 96 hr after drug administration. Intravenous LAAM produced significant subjective and physiological effects that appeared within 5 min, whereas the effects of oral LAAM appeared more slowly within 1 to 2 hr after drug administration. Pharmacokinetic data indicate that the immediate effects of intravenous LAAM are largely attributable to the parent drug rather than the active metabolites, nor-LAAM and dinor-LAAM. LAAM produced prototypic opioid agonist effects (i.e., miosis, subjective ratings of high, nodding) that were of equal magnitude across routes, dose-related and of long duration (up to 60 hr). These data are in contrast to previous clinical reports and indicate that LAAM produces effects of immediate onset when administered parenterally, which suggests that intravenous LAAM possesses greater abuse potential than previously believed.
Mechanotransduction is enhanced by the synergistic action of heterotypic cell interactions and TGF‐β1

With the use of planar substrates and collagen gels, the field of mechanotransduction has focused on the role of extracellular matrix stiffness, mechanical tension, and TGF‐β1 in generating a more contractile fibroblast. However, little is known about the role of cell‐cell interactions in inducing cellular contraction. We used 3‐dimensional self‐assembled microtissues, in which cell‐cell interactions dominate, and a recently developed cell power assay (an assay for mechanotransduction) to quantify the effects of TGF‐β1 vs. the heterotypic cell interface on the power exerted by pure normal human fibroblast (NHF) and pure rat hepatocyte 35 (H35) microtissues and their mixes. As a control, we found that TGF‐β1 only doubled the power output of pure NHF and pure H35 microtissues, whereas the heterotypic environment resulted in a 5‐fold increase in cell power (0.24±0.05 to 1.17±0.13 fJ/h). Seeding TGF‐β1‐treated NHFs with untreated H35 cells demonstrated that the heterotypic environment and TGF‐β1 synergistically increase cell power by 22× by maximizing heterotypic cell interactions. Using a mathematical simulation of stress generation, we showed that tensile forces can be enhanced by heterotypic cell interactions. These data render a new understanding of how heterotypic cell interactions may increase cellular force generation during wound healing. —Youssef, J., Chen, P., Shenoy, V. B. , Morgan, J. R. Mechanotransduction is enhanced by the synergistic action of heterotypic cell interactions and TGF‐β1. FASEB J. 26, 2522‐2530 (2012). www.fasebj. org
Physical activity and body mass index among women who have experienced infertility

Introduction The experience of infertility is a common medical condition in the developing countries. The aim of this retrospective epidemiologic study was to determine fertility status and parity in Babol, Iran and then identify physical activity and body mass index (BMI) among women who have experienced infertility. Material and methods A total of 1,081 women aged 20-45 years were selected using cluster sampling. The current physical activity was measured using the original International Physical Activity Questionnaire short form. Lifestyle factors were compared between those who had experienced infertility (n = 168) and the rest of the women. A face-to-face household interview was conducted using a specially designed interview questionnaire. Results After adjusting for suspected confounding factors, women with infertility experience had a 4.8-fold increased risk of obesity (OR = 2.02, CI = 0.70, 5.84) and almost a 3.8-fold increased risk of being overweight (OR = 2.11, CI = 0.72, 6.17) compared to women without infertility. No significant differences were found in Met-minutes of sedentary activity, intensity of walking, moderate, vigorous, and total physical activity, self-reported dietary intake, exercise, and level of physical activity between women with and without experience of infertility. Conclusions Since both obesity and infertility are increasing public health issues in Iranian women, more attention should be paid to lifestyle behaviors, especially gaining weight in women who have experienced infertility.
Knowledge translation: An interprofessional approach to integrating a pain consult team within an acute care unit

ABSTRACT Management of pain in the frail elderly presents many challenges in both assessment and treatment, due to the presence of multiple co-morbidities, polypharmacy, and cognitive impairment. At Baycrest Health Sciences, a geriatric care centre, pain in its acute care unit had been managed through consultations with the pain team on a case-by-case basis. In an intervention informed by knowledge translation (KT), the pain specialists integrated within the social network of the acute care team for 6 months to disseminate their expertise. A survey was administered to staff on the unit before and after the intervention of the pain team to understand staff perceptions of pain management. Pre- and post-comparisons of the survey responses were analysed by using t-tests. This study provided some evidence for the success of this interprofessional education initiative through changes in staff confidence with respect to pain management. It also showed that embedding the pain team into the acute care team supported the KT process as an effective method of interprofessional team building. Incorporating the pain team into the acute care unit to provide training and ongoing decision support was a feasible strategy for KT and could be replicated in other clinical settings.
Pathogenic poxviruses reveal viral strategies to exploit the ErbB signaling network

Virulence of poxviruses, the causative agents of smallpox, depends on virus‐encoded growth factors related to the mammalian epidermal growth factor (EGF). Here we report that the growth factors of Shope fibroma virus, Myxoma virus and vaccinia virus (SFGF, MGF and VGF) display unique patterns of specificity to ErbB receptor tyrosine kinases; whereas SFGF is a broad‐specificity ligand, VGF binds primarily to ErbB‐1 homodimers, and the exclusive receptor for MGF is a heterodimer comprised of ErbB‐2 and ErbB‐3. In spite of 10‐ to 1000‐fold lower binding affinity to their respective receptors, the viral ligands are mitogenically equivalent or even more potent than their mammalian counterparts. This remarkable enhancement of cell growth is due to attenuation of receptor degradation and ubiquitination, which leads to sustained signal transduction. Our results imply that signal potentiation and precise targeting to specific receptor combinations contribute to cell transformation at sites of poxvirus infection, and they underscore the importance of the often ignored low‐affinity ligand–receptor interactions.
Real-time specific beamforming applied to motion trajectory estimation in ultrasound imaging

In this paper, a motion trajectory estimation method with specific ultrasound RF images is proposed. These specific RF images with axial and lateral oscillations are obtained using an unconventional beamforming technique. The first aim of this paper is to present an experimental setup of real-time implementation of this beamforming technique providing phase images sensitive to subpixel motions. The second aim of the paper is to introduce a method of trajectory estimation. The proposed multi-frame motion estimation method uses a spatio-temporal prediction technique and an analytic estimator of 2-D spatial shifts dedicated to phase images.
Gentamicin interaction with Pseudomonas aeruginosa cell envelope

Gentamicin, an aminoglycoside antibiotic known to inhibit protein synthesis, had a detrimental effect on the integrity of the cell wall of Pseudomonas aeruginosa ATCC 9027 (a susceptible strain) as shown by electron microscopy using negative-staining, thin-sectioning, and freeze-fracture techniques. The disruption occurred in a sequential manner, moving from the outer membrane to the inner membrane, and could result in lysis of the cell. During this process the outer membrane lost 34% of its total protein and 30% of its lipopolysaccharide (measured as 2-keto-3-deoxyoctonate) upon exposure to 25 micrograms of gentamicin per ml for 15 min. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the outer membrane proteins showed altered banding patterns after exposure to gentamicin. Atomic absorption spectrophotometry revealed a decrease in magnesium and calcium content (18 and 38%, respectively) in the cell envelopes after gentamicin treatment. It is proposed that gentamicin displaces essential metal cations within the outer membrane, consequently destabilizing and extracting organic constituents. Small transient holes are thereby produced which make the outer membrane more permeable to the antibiotic and which expose the protoplast to high concentrations of gentamicin. This membrane effect may contribute to the effects of protein synthesis inhibition during the killing process.