Miracle-dz/UniFAR-FTUs huggingface dataset:
Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli

The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to rhamnulose kinase for rhaB, a 400-amino-acid polypeptide corresponding to rhamnose isomerase for rhaA, and a 274-amino-acid polypeptide corresponding to rhamnulose-1-phosphate aldolase for rhaD. Transcriptional fusions of the three putative promoter regions to lacZ showed that only the rhaB leader region acted as a promoter, as indicated by the high beta-galactosidase activity induced by rhamnose, while no significant activity from the rhaA and rhaD constructions was detected. The rhaB transcription start site was mapped to -24 relative to the start of translation. Mutations in the catabolic genes were used to show that L-rhamnose may directly induce rhaBAD transcription.
A Comprehensive Structure–Function Analysis of Arabidopsis SNI1 Defines Essential Regions and Transcriptional Repressor Activity[W][OA]

The expression of systemic acquired resistance (SAR) in plants involves the upregulation of many Pathogenesis-Related (PR) genes, which work in concert to confer resistance to a broad spectrum of pathogens. Because SAR is a costly process, SAR-associated transcription must be tightly regulated. Arabidopsis thaliana SNI1 (for Suppressor of NPR1, Inducible) is a negative regulator of SAR required to dampen the basal expression of PR genes. Whole genome transcriptional profiling showed that in the sni1 mutant, Nonexpresser of PR genes (NPR1)–dependent benzothiadiazole S-methylester–responsive genes were specifically derepressed. Interestingly, SNI1 also repressed transcription when expressed in yeast, suggesting that it functions as an active transcriptional repressor through a highly conserved mechanism. Chromatin immunoprecipitation indicated that histone modification may be involved in SNI1-mediated repression. Sequence comparison with orthologs in other plant species and a saturating NAAIRS-scanning mutagenesis of SNI1 identified regions in SNI1 that are required for its activity. The structural similarity of SNI1 to Armadillo repeat proteins implies that SNI1 may form a scaffold for interaction with proteins that modulate transcription.
A precoding technique based on time reversal for single-carrier IDMA systems

According to the high complexity of pre-coder in single-carrier interleave-division multiple access (IDMA) systems, in this paper, we propose a precoding technique based on time-reversal (TR) for single-carrier IDMA systems. The proposed scheme gets the channel impulse responses (CIR) through time-division duplexing (TDD) mode, and uses the time-reversed version of the CIR to pre-coder the transmitted signal at transmitter. The receiver adopts the iterative Linear Minimum Mean Square Error (LMMSE) frequency domain equalization (FDE) algorithm. Thus the pre-coder of transmitter is simple and easy to implement as compared with water filling pre-coder and optimal pre-coder, and the receiver can be simplified by canceling complex computation. The simulation results show that the performance of TR pre-coder is close to that of optimized pre-coder and that of water filling in single-carrier IDMA systems based on iterative LMMSE-FDE algorithm when signal to noise ratio (SNR) is higher than 3.5dB. The simulation results also demonstrate that TR pre-coder has potential advantages.
Hoodigogenin A from Hoodia gordonii

The title molecule (systematic name: 12-O-β-tigloyl-3β,14β-dihydroxypregn-5-en-20-one), C26H38O5, isolated from aerial parts of Hoodia gordonii, has its steroid A and C rings in chair conformations, its B ring in a half-chair conformation, and its five-membered ring in an envelope conformation. The OH group at the C/D ring junction forms an intramolecular hydrogen bond with the keto substituent. The OH group on the A ring forms an intermolecular hydrogen bond with the tiglate C=O group, propagating [010] chains in the crystal structure.
Aligning Time Lapses of Stars Using Contextual Information

We propose a method of video stabilization for a video time lapse of stars by utilizing a contextual shape descriptor to match corresponding stars on different frames. The descriptor is based from Belongie, Malik, and Puzicha [4] using log-polar bins to mask onto an image. Contextual landmark tracking is not well used in video stabilization because of it’s inefficiency and computational expense. By incorporating rotational invariance, contextual landmark tracking can possibly be proved to be worth the expense for some examples and extend applicability. While landmark tracking is not commonly used, it can be proved useful if purely local information is insufficient. Our challenges with the data were due to points disappearing and reappearing in different frames, rotating off the frame, and also that each point looked alike. In terms of our rotationally invariant descriptor, we looked into Bhonsle and Klinsman [2] for their uses of the Fast Fourier Transform. Our rotationally invariant descriptor takes an initial frame and identifies the same point in another frame whose points are completely rotated from the original. When we make that match, it will prove that our solution is effective.
The impact of sink location on the performance, throughput and energy efficiency of the WSNs

Wireless Sensor network (WSN) is regarded as the most potential approach for keeping track of the physical location wherein monitoring and checking is critical. The efficiency of sensor networks strongly depends on the routing protocols used. In this paper, we examine and evaluate the impact of the divergent sink location on one of the earliest and most significant hierarchical routing protocol, LEACH. Various simulations such as the packets send to the sink, number of dead nodes in each round and the overall energy dissipation of the network are carried out to observe the performance of the protocol such as energy consumption and overall performance of the network. The result of simulation indicates that the location of base station in the centre of the network and the centre of the network's portion having highest number of nodes are the optimum choices to increase the efficiency and overall performance of the of the network.
Fast and Accurate 3D Scanning Using Coded Phase Shifting and High Speed Pattern Projection

We describe a structured light phase measuring triangulation technique extending the conventional four-step phase shift sequence with embedded information suited to assist the phase unwrapping process. Using the embedded information, we perform automatic phase unwrapping in the presence of discontinuous or isolated surfaces without extending the length of the phase shift sequence or requiring stereo cameras. We demonstrate the application of the proposed method using a novel structured light projector capable of extraordinarily high projection frequencies and pattern resolution, as well as grayscale quantization. Using high speed cameras, we demonstrate 3D measurements at 20ms total acquisition time for both mono- and stereoscopic camera configurations.
Childhood Poverty, Cumulative Risk Exposure, and Mental Health in Emerging Adults

One out of four American children is born into poverty, but little is known about the long-term mental health implications of early deprivation. The more time in poverty from birth to age 9, the worse mental health is for these individuals as emerging adults (n = 196, M = 17.30 years, 53% male). These results maintained independently of concurrent, adult income levels for self-reported externalizing symptoms and a standard learned helplessness behavioral protocol, but internalizing symptoms were unaffected by childhood poverty. We then demonstrate that part of the reason why early poverty exposure is harmful to mental health among emerging adults is because of elevated cumulative risk exposure assessed at age 13. The significant prospective longitudinal relations between early childhood poverty and externalizing symptoms plus learned helplessness behavior are mediated, in part, by exposure to a confluence of psychosocial (violence, family turmoil, child separation from family) and physical (noise, crowding, substandard housing) risk factors during adolescence.
Hierarchic Agglomerative Clustering Methods for Automatic Document Classification

This paper considers the classifications produced by application of the single linkage, complete linkage, group average and Ward clustering methods to the Keen and Cranfield document test collections. Experiments were carried out to study the structure of the hierarchies produced by the different methods, the extent to which the methods distort the input similarity matrices during the generation of a classification, and the retrieval effectiveness obtainable in cluster based retrieval. The results would suggest that the single linkage method, which has been used extensively in previous work on document clustering, is not the most effective procedure of those tested, although it should be emphasized that the experiments have used only small document test collections.
An Analysis of Texture Measures in PCA-Based Unsupervised Classification of SAR Images

In single-band single-polarized SAR images, intensity and texture are the information source available for unsupervised land cover classification. Every textural feature measure identifies texture patterns by different approaches. For efficient land cover classification, textural measures have to be chosen suitably. Therefore, in this letter, the role of various intensity and textural measures is analyzed for their discriminative ability for unsupervised SAR image classification into various land cover types like water, urban, and vegetation areas. To make the algorithm adaptable, these textural features are fused using principal component analysis (PCA), and principal components are used for classification purposes. To highlight the effectiveness of PCA, the difference between PCA- and non-PCA-based classifications is also analyzed. Analysis of the role of texture measures for unsupervised classification of real-world SAR data with application of PCA is presented in this letter. The analysis of how every individual feature measure contributes for classification process is presented, and then, textural measures for a feature set are chosen according to their role in improving classification accuracy. By analysis, it is observed that the feature set comprising mean, variance, wavelet components, semivariogram, lacunarity, and weighted rank fill ratio provides good classification accuracy of up to 90.4% than by using individual textural measures, and this increased accuracy justifies the complexity involved in the process.
Ergodic exploration with stochastic sensor dynamics

Ergodic exploration has been shown to be an effective framework for autonomous sensing and exploration. The objective of ergodic control is to minimize the difference between the distribution of the time-averaged sensor trajectory and a spatial probability distribution function representing information density. Therefore, the time a sensor spends sampling a particular region is manipulated to correspond to the anticipated information density of that region. This paper introduces a trajectory optimization approach for ergodic exploration in the presence of stochastic sensor dynamics. The stochastic differential dynamic programming algorithm is formulated in the context of ergodic exploration. Numerical studies demonstrate the proposed framework's ability to mitigate stochastic effects.
Inhalation of Concentrated Ambient Particulate Matter near a Heavily Trafficked Road Stimulates Antigen-Induced Airway Responses in Mice

Abstract The goal of this study was to test the following hypotheses:(1) exposure to mobile emissions from mobile sources close to a heavily trafficked roadway will exacerbate airway inflammation and allergic airway responses in a sensitized mouse model, and (2) the magnitude of allergic airway disease responses will decrease with increasing distance from the roadway. A particle concentrator and a mobile exposure facility were used to expose ovalbumin (OVA)-sensitized BALB/c mice to purified air and concentrated fine and concentrated ultrafine ambient particles at 50 m and 150 m downwind from a roadway that was heavily impacted by emissions from heavy duty diesel-powered vehicles. After exposure, we assessed interleukin (IL)-5, IL-13, OVA-specific immunoglobulin E, OVA-specific immunoglobulin G1, and eosinophil influx as biomarkers of allergic responses and numbers of polymorphonuclear leukocytes as a marker of inflammation. The study was performed over a two-year period, and there were differences in the concentrations and compositions of ambient particulate matter across those years that could have influenced our results. However, averaged over the two-year period, exposure to concentrated ambient particles (CAPs) increased the biomarkers associated with airway allergies (IL-5, immunoglobulin E, immunoglobulin G1 and eosinophils). In addition, mice exposed to CAPs 50 m downwind of the roadway had, on the average, greater allergic responses and showed greater indications of inflammation than did mice exposed to CAPs 150 m downwind. This study is consistent with the hypothesis that exposure to CAPs close to a heavily trafficked roadway influenced allergic airway responses.
A multiple instances approach to improving keyword spotting on historical Mongolian document images

For keyword spotting of historical Mongolian document images, when user provides different instance image for the same query keyword, the performance will vary a lot. This paper proposed an approach to solving the above problem. Particularly, the whole procedure of keyword spotting is divided into two stages. The main task of the first stage is to generate multiple ranking lists for a query keyword. And the aim of the second stage is to merge the multiple ranking lists to form a final ranking. In the first stage, the ranking list of one query keyword is firstly returned by traditional image matching and then a number of instances for the query keyword are obtained using pseudo relevant feedback. Next, each instance of the query keyword can return the corresponding ranking list separately. In the second stage, the multiple ranking lists from the multiple instances of the query keyword are combined by the data fusion technique. The final ranking will be taken as the retrieval results of the query keyword. The experimental results show that the proposed approach can significantly improve the performance of keyword spotting for the historical Mongolian document images.
Quality of life in stage II breast cancer: an instrument for clinical trials.

A questionnaire has been developed for use as an outcome measure in clinical trials of adjuvant chemotherapy in women with stage II breast cancer. The selection of items for this Breast Cancer Chemotherapy Questionnaire (BCQ) was based on the problems and experiences felt to be most important by women undergoing adjuvant chemotherapy. The BCQ consists of 30 questions that focus on loss of attractiveness, fatigue, physical symptoms, inconvenience, emotional distress, and feelings of hope and support from others. The BCQ, other instruments that evaluate quality-of-life (Spitzer, Karnofsky, and Rand), and patient and physician global assessments were administered serially to 418 patients taking part in a randomized trial comparing a 12-week regimen and a 36-week regimen of adjuvant chemotherapy. The validity of the BCQ is supported by its correlation with the Rand Emotional (r = .58), Rand Physical (r = .60), and Spitzer (r = .62) questionnaires. The BCQ correlated more strongly with global ratings of both physical and emotional function by the patients and their physicians than the other instruments. A comparison of the quality-of-life outcomes of patients in the two treatment groups in the period beyond 3 months after initiation of treatment, when one group had completed the treatment course and the other was still on treatment, revealed that the BCQ and Karnofsky were the only instruments able to demonstrate differences between the groups (P less than .0001). Hence, the BCQ is a valid and responsive method of assessing treatment-related morbidity in patients receiving adjuvant chemotherapy for stage II breast cancer.
New Beamforming and Relay Selection for Two-Way Decode-and-Forward Relay Networks

Multiple access broadcasting (MABC) is a spectral efficient protocol in a two-way relay channel (TWRC). The drawback of this protocol in decode-and-forward (DF) mode is decoding complexity at the relay side. Erroneous decoding at the relay can lead to error propagation that degrades the performance of all relay networks operating in the DF mode. In this paper, a maximum likelihood (ML) detector at the multiple-antenna relay is used, and it is proved that this decoding rule can achieve full diversity order at the multiple access (MAC) phase of the MABC protocol. Then, a new beamforming algorithm named as channel alignment is proposed, and it is shown that it achieves full diversity order. A new relay selection algorithm is proposed, and a tight upper bound for the bit error rate (BER) of this scheme is derived. It is proved that it achieves full diversity order. The analytical results and BER performance of the proposed schemes are investigated and compared through simulations.
Analysis of occupation technique & ability in network engineering using recruitment information

This paper carries out statistics and correlations between the job positions and job responsibilities, job positions and experiences and qualifications based on the web recruitment information in network engineering. This is very helpful for students or job seeking people to know what knowledge should be understood, familiar with or known about, and is advisable for managers in school or in training institutes to realize how many courses should be set up and what courses should be paid more attention or be priority.
Design Automation Conference Scholarship Awards

The objective of the ACSEE Scholarship program is to increase the pool of professionals in Electrical Engineering and Computer Science from under-represented groups (Women, African American, Hispanic, Native American, and Physically Challenged). In 1989, ACM Special Interest Group on Design Automation (SIGDA) began providing the program. Beginning in 1993, the Design Automation Conference provides the funds for the scholarship and SIGDA continues to administer the program for DAC. DAC funds two $4000 scholarships renewable up to 5 years to graduating high school seniors. The former International Daisy User Group funds one $1000 one-time-only scholarship.
Influence of Physical Education on Moderate-to-Vigorous Physical Activity of Urban Public School Children in St. Louis, Missouri, 2011–2014

We quantified the moderate-to-vigorous physical activity (MVPA, heart rate ≥140 bpm) of urban public elementary school children on school days with and school days without physical education (PE) class by using continuous heart rate monitoring. The heart rate of 81 students (93.8% black) in grades 3 and 5 was recorded in 15-second intervals. On the basis of 575 school-day observations (mean 7.1 days/student), students accumulated 44.4 (standard deviation [SD], 34.4) minutes of MVPA on days with PE and 30.6 (SD, 29.9) MVPA minutes on days without PE (P < .001). School policies should promote daily PE to help children in under-resourced areas achieve the recommended 60 minutes per day of MVPA.
A murine monoclonal antibody against Kx protein which reacts also with β‐spectrin

Kx is a polytopic membrane protein of human erythrocytes carrying the Kx blood group antigen, which is deficient in rare patients with McLeod syndrome. Kx is disulphide bond linked to the Kell glycoprotein, which is a bitopic type II membrane protein carrying the Kell blood group antigen. Mice immunized with a synthetic peptide predicted to be located on the second external loop of Kx produced a monoclonal antibody called 3E12 which does not recognize red cells with common Kell phenotype by agglutination and flow cytometry. 3E12 recognizes the Kx protein and the spectrin β‐chain on western blots, the affinity for these two proteins being lowered with increasing ionic strength. Linear epitopes recognized by 3E12 are E116EIEKE121 and L484AQELEKE491 on the Kx protein and spectrin β‐chain, respectively. To quantify the relative amount of Kx in Empigen BB extracts of red cell membranes, an ELISA for Kx was set up which showed conclusively that (i) there is less Kx in membranes of K0 individuals (lacking the Kell glycoprotein) than in membranes of common individuals, and (ii) that all common individuals, typed as K+k–, K–k+ and K+k+, have the same amount of Kx on their red cell membranes. When an erythrocyte membrane detergent extract from one K0 individual was chromatographed on an immobilized 3E12 column, a minute amount of authentic Kell glycoprotein was recovered in acid eluted fractions, indicating that at least the K0 individual under study may still produce some Kell protein.
In Human Leukemia Cells Ephrin-B–Induced Invasive Activity Is Supported by Lck and Is Associated with Reassembling of Lipid Raft Signaling Complexes

Proteins of the ephrin-B group operate in nonlymphoid cells through the control of their migration and attachment, and are crucial for the development of the vascular, lymphatic, and nervous systems. Ephrin-B activity is deregulated in various nonlymphoid malignancies; however, their precise role in cancer has only started to be addressed. We show here that ephrin-B1, a member of the ephrin-B group, is expressed in pediatric T-cell leukemias, including leukemia cell line Jurkat. Treatment of Jurkat cells with ephrin-B–stimulating EphB3 enhances ephrin-B1 phosphorylation and induces its relocalization into lipid rafts. These events are mediated by the T lineage–specific kinase, Lck, as ephrin-B1 phosphorylation and lipid raft association are blocked in the Lck-deficient clone of Jurkat, JCAM1.6. Ephrin-B1 also induces colocalization of the CrkL and Rac1 cytoskeleton regulators and initiates in leukemic cells a strong repulsive response. The absence of Lck blocks ephrin-B1–induced signaling and repulsion, confirming the essential role for Lck in ephrin-B1–mediated responses. This shows a new role for ephrin-B1 in the regulation of leukemic cells through the Lck-dependent Rac1 colocalization with its signaling partner, CrkL, in lipid rafts. In agreement with its repulsive action, ephrin-B1 seems to support metastatic properties of leukemic cells, as suppression of ephrin-B1 signaling inhibits their invasiveness. Because ephrin-B1–activating EphB proteins are ubiquitously expressed, our findings suggest that ephrin-B1 is likely to play an important role in the regulation of malignant T lymphocytes through the control of lipid-raft–associated signaling, adhesion, and invasive activity, and therefore may represent a novel target for cancer treatment. (Mol Cancer Res 2008;6(2):291–305)
Local covariance matrices for improved target detection performance

Our research goals in hyperspectral point target detection have been to develop a methodology for algorithm comparison and to advance point target detection algorithms through the fundamental understanding of spatial/spectral statistics. In this paper, we demonstrate improved target detection performance by making better estimates of the covariance matrix. We develop a new type of local covariance matrix which can be implemented in Principal Component space which shows improved performance based on our metrics.