Miracle-dz/UniFAR-FTUs huggingface dataset:
Baculoviral Expression of Presumptive OP-Resistance Mutations in BmAChE1 of Rhipicephalus (Boophilus) microplus (Ixodida: Ixodidae) and Biochemical Resistance to OP Inhibition

Abstract The southern cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini), transmits bovine babesiosis and anaplasmosis, and is endemic to Mexico, Latin and South America. Rhipicephalus (B.) microplus infestations within the United States are a continuing threat to U.S. cattle producers. An importation barrier between Texas and Mexico keeps the ticks from re-entering the United States. All cattle imported into the United States are dipped in an organophosphate (OP) acaricide and hand inspected for presence of ticks. Tick resistance has developed to most available acaricides, including coumaphos, the OP used in the cattle dip vats. OP-resistance can result from one or more mutations in the gene encoding the enzyme, acetylcholinesterase (AChE), resulting in production of an altered AChE resistant to OP inhibition. Previous research reported a large number of BmAChE1 mutations associated with OP resistance. We report baculovirus expression of recombinant tick BmAChE1 (rBmAChE) enzymes containing a single resistance-associated mutation, to assess their contribution to OP inhibition resistance. Surprisingly, of the naturally occurring BmAChE1 resistance-associated mutations, only D188G resulted in markedly reduced sensitivity to OP-inhibition suggesting that OP-insensitivity in BmAChE1 may result from the D188G mutation, or may possibly result from multiple mutations, each contributing a small decrease in OP sensitivity. Furthermore, an OP-insensitivity mutation (G119S) found in mosquitoes was expressed in rBmAChE1, resulting in 500-2000-fold decreased sensitivity to OP inhibition. Recombinant BmAChE1 with the G119S mutation demonstrated the lack of any structural prohibition to broad and high-level OP-insensitivity, suggesting potential increases in tick OP-resistance that would threaten the U.S. importation barrier to ticks.
Evaluation of the signal quality of wrist-based photoplethysmography

Objective: Wearable devices with embedded photoplethysmography (PPG) sensors enable continuous monitoring of cardiovascular activity, allowing for the detection cardiovascular problems, such as arrhythmias. However, the quality of wrist-based PPG is highly variable, and is subject to artifacts from motion and other interferences. The goal of this paper is to evaluate the signal quality obtained from wrist-based PPG when used in an ambulatory setting. Approach: Ambulatory data were collected over a 24 h period for 10 elderly, and 16 non-elderly participants. Visual assessment is used as the gold standard for PPG signal quality, with inter-rater agreement evaluated using Fleiss’ Kappa. With this gold standard, 5 classifiers were evaluated using a modified 13-fold cross-validation approach. Main results: A Random Forest quality classification algorithm showed the best performance, with an accuracy of 74.5%, and was then used to evaluate 24 h long ambulatory wrist-based PPG measurements. Significance: In general, data quality was high at night, and low during the day. Our results suggest wrist-based PPG may be best for continuous cardiovascular monitoring applications during the night, but less useful during the day unless methods can be identified to improve low quality signal segments.
The Secret to Popular Chinese Web Novels: A Corpus-Driven Study

What is the secret to writing popular novels? The issue is an intriguing one among researchers from various ﬁelds. The goal of this study is to identify the linguistic features of several popular web novels as well as how the textual features found within and the overall tone interact with the genre and themes of each novel. Apart from writing style, non-textual information may also reveal details behind the success of web novels. Since web ﬁction has become a major industry with top writers making millions of dollars and their stories adapted into published books, determining essential elements of “publishable” novels is of importance. The present study further examines how non-textual information, namely, the number of hits, shares, favorites, and comments, may contribute to several features of the most popular published and unpublished web novels. Findings reveal that keywords, function words, and lexical diversity of a novel are highly related to its genres and writing style while dialogue proportion shows the narration voice of the story. In addition, relatively shorter sentences are found in these novels. The data also reveal that the number of favorites and comments serve as signiﬁcant predictors for the number of shares and hits of unpublished web novels, respectively; however, the number of hits and shares of published web novels is more unpredictable. 2012 ACM Subject Classiﬁcation
Protein structure prediction: challenging targets for CASP10

Functional characterization of proteins being one of the major issues in molecular biology is still unsolved due to several resource and technical limitations of experimental structure determination methods. A suitable methodology for accurate prediction of protein confirmations simply from sequence is therefore emerging as the primary modeling goal of researchers today. Global blind protein structure prediction summit, entitled Critical Assessment of Structure Prediction (CASP), critically assesses the modeling methodologies, to track our algorithmic path development. But our success is still impeded by incompetent modeling methodologies and several key technical lacunas. There is still a great need to focus some key issues for bridging the major though considered trivial gaps, in the upcoming CASP to pave and demarcate our correct way of developing a consistently accurate prediction methodology in the near future. Major problems resulting in divergence of our predicted models from their actual native states are thus highlighted with suggested more stringent and reliable assessment considerations in the CASP test.
Cherokee Syllabary Texts: Digital Documentation and Linguistic Description

The Digital Archive of American Indian Languages Preservation and Perseverance (DAILP) is an innovative language revitalization project that seeks to provide digital infrastructure for the preservation and study of endangered languages among Native American speech communities. The project’s initial goal is to publish a digital collection of Cherokee-language documents to serve as the basis for language learning, cultural study, and linguistic research. Its primary texts derive from digitized manuscript images of historical Cherokee Syllabary texts, a written tradition that spans nearly two centuries. Of vital importance to DAILP is the participation and expertise of the Cherokee user community in processing such materials, speciﬁcally in Syllabary text transcription, romanization, and translation activities. To support the study and linguistic enrichment of such materials, the project is seeking to develop tools and services for the modeling, annotation, and sharing of DAILP texts and language data.
On the design of high-performance wide-band continuous-time sigma-delta converters using numerical optimization

Continuous-time (CT) sigma-delta (/spl Sigma//spl Delta/) modulators are growing increasingly popular in wide-band analog-digital conversion. High orders of quantization noise shaping, and multibit quantizers, are used to compensate for the low oversampling ratios in wide-band applications. These, however, add circuit complexities and excess loop delay that are detrimental to the /spl Sigma//spl Delta/ control loop. This paper presents an exact mathematical analysis technique, based on the CT-discrete time equivalence, that can take these effects into account. A design-by-optimization approach based on that analysis is used to compensate for these effects, avoid intractability issues and to gain flexibility in the design. It is also shown that it is advantageous not to fix the position of the quantization noise-shaping zeros in the signal band.
Translation-Based Dictionary Alignment for Under-Resourced Bantu Languages

Despite a large number of active speakers, most Bantu languages can be considered as underor lessresourced languages. This includes especially the current situation of lexicographical data, which is highly unsatisfactory concerning the size, quality and consistency in format and provided information. Unfortunately, this does not only hold for the amount and quality of data for monolingual dictionaries, but also for their lack of interconnection to form a network of dictionaries. Current endeavours to promote the use of Bantu languages in primary and secondary education in countries like South Africa show the urgent need for high-quality digital dictionaries. This contribution describes a prototypical implementation for aligning Xhosa, Zimbabwean Ndebele and Kalanga language dictionaries based on their English translations using simple string matching techniques and via WordNet URIs. The RDF-based representation of the data using the Bantu Language Model (BLM) and – partial – references to the established WordNet dataset supported this process significantly. 2012 ACM Subject Classification Information systems → Resource Description Framework (RDF); Computing methodologies → Phonology / morphology; Information systems → Dictionaries
Modulating Lateral Geniculate Nucleus Neuronal Firing for Visual Prostheses: A Kalman Filter-Based Strategy

Developing visual prostheses that target inner brain structures along the visual pathway represent a new hope for patients with completely damaged early visual pathway sites. One of the major challenges in the development of subcortical and cortical visual prostheses is tuning electrical stimulation that could optimally induce desired visual percepts. In this paper, we propose a Kalman filter-based strategy that could be used to identify electrical stimulation patterns that mimic a specific visual input for thalamic visual prostheses. We demonstrate the performance of the proposed strategy using a population of lateral geniculate nucleus neurons modeled using an adapted generalized non-linear model. A mean correlation of 0.69 is obtained between visually evoked and electrically evoked responses–driven using the proposed strategy–for an optimal electrode-placement setup. In addition, we demonstrate the performance for a random electrode-placement setup in which a mean correlation of 0.26 is obtained. For this latter setup, our analysis reveals an inversely proportional relationship between the obtained correlation and the distance between each neuron and the nearest electrode. The proposed strategy could be thus utilized to tune and enhance the performance of thalamic visual prostheses as well as other prosthesis systems.
The Effect of Mouse Strain, Sex, and Carcinogen Dose on Toxicity and the Development of Lung Dysplasia and Squamous Cell Carcinomas in Mice

In order to translate new treatments to the clinic, it is necessary to use animal models that closely recapitulate human disease. Lung cancer develops after extended exposure to carcinogens. It has one of the highest mutation rates of all cancer and is highly heterogenic. Topical treatment with N-nitrosotris-(2-chloroethyl)urea (NTCU) induces lung squamous cell carcinoma (SCC) with nonsynonymous mutation rates similar to those reported for human non–small cell lung cancer. However, NTCU induces lung cancer with variable efficacy and toxicity depending on the mouse strain. A detailed characterization of the NTCU model is needed. We have compared the effect of three different NTCU doses (20, 30, and 40 mmol/L) in female and male of NIH Swiss, Black Swiss, and FVB mice on tumor incidence, survival, and toxicity. The main findings in this study are (1) NIH Swiss mice present with a higher incidence of SCC and lower mortality compared with Black Swiss and FVB mice; (2) 30 mmol/L NTCU dose induces SCC at the same rate and incidence as the 40 mmol/L dose with lower mortality; (3) female mice present higher grade and incidence of preinvasive lesions and SCC compared with males; (4) NTCU-induced transformation is principally within the respiratory system; and (5) NTCU treatment does not affect the ability to elicit a specific adaptive immune response. This study provides a reference point for experimental designs to evaluate either preventive or therapeutic treatments for lung SCC, including immunotherapies, before initiating human clinical trials.
Towards Occlusion Handling: Object Tracking With Background Estimation

The appearance model of the target needs to be updated for online single object tracking. However, the variation of the observation can be caused by active appearance change of the target, or the occlusion from the background. For the former case, we should update the appearance model and for the latter, the current model should be preserved. In this paper, we distinguish these two cases and resist the impact from heavy occlusion by estimating the background in the scene with moving cameras, while retaining the adaptivity to stationary cameras at the same time. The proposed method formulates the background as a Gaussian model and the target is determined in a coarse-to-fine manner. Experimental results demonstrate that our method achieves competitive results in the sequences with appearance changes and outperforms the state-of-the-art algorithms in dealing with complex occlusions.
Temporal analyses of the neuropathogenesis of a West Nile virus infection in mice

A West Nile virus (WNV) infection in humans can produce neurological symptoms including acute flaccid paralysis, encephalitis, meningitis and myelitis. To investigate the pathogenesis of WNV in the peripheral and the central nervous system (PNS and CNS), the authors used a murine footpad inoculation model of WNV infection. Survival curves of virus-infected animals of ages 4- to 6-weeks-old demonstrated age-dependent mortality where older animals (6-weeks-old) had a higher mortality rate compared to younger animals (4- and 5-weeks-old). The mice that survived the virus infection formed WNV-reactive antibodies, confirming viral infection and clearance. The localization of viral RNA (vRNA) and antigen in infected murine tissues was investigated using TaqMan and immunohistochemistry (IHC) respectively. During a nine day infection, vRNA levels in the spinal cord and brainstem fluctuated, suggesting early viral clearance from these tissues by days 3–4 p.i. with later re-introduction. Viral antigens detected using IHC were primarily observed in three main regions of the brain: cortex, hippocampus and brainstem. Additionally, the dorsal root ganglion neurons of the PNS stained positive for viral antigens. These data are consistent with multiple routes of neuroinvasion following a peripheral inoculation of virus and do not preclude the previous observation that virus-infected peripheral neurons can introduce virus into the CNS by a retrograde transport mechanism.
Can we use structural lesions seen on MRI of the sacroiliac joints reliably for the classification of patients according to the ASAS axial spondyloarthritis criteria? Data from the DESIR cohort

Objectives Investigating the utility of adding structural lesions seen on MRI of the sacroiliac joints to the imaging criterion of the Assessment of SpondyloArthritis (ASAS) axial SpondyloArthritis (axSpA) criteria and the utility of replacement of radiographic sacroiliitis by structural lesions on MRI. Methods Two well-calibrated readers scored MRI STIR (inflammation, MRI-SI), MRI T1-w images (structural lesions, MRI-SI-s) and radiographs of the sacroiliac joints (X-SI) of patients in the DEvenir des Spondyloarthrites Indifférenciées Récentes cohort (inflammatory back pain: ≥3 months, <3 years, age <50). A third reader adjudicated MRI-SI and X-SI discrepancies. Previously proposed cut-offs for a positive MRI-SI-s were used (based on <5% prevalence among no-SpA patients): erosions (E) ≥3, fatty lesions (FL) ≥3, E/FL ≥5. Patients were classified according to the ASAS axSpA criteria using the various definitions of MRI-SI-s. Results Of the 582 patients included in this analysis, 418 fulfilled the ASAS axSpA criteria, of which 127 patients were modified New York (mNY) positive and 134 and 75 were MRI-SI-s positive (E/FL≥5) for readers 1 and 2, respectively. Agreement between mNY and MRI-SI-s (E/FL≥5) was moderate (reader 1: κ: 0.39; reader 2: κ: 0.44). Using the E/FL≥5 cut-off instead of mNY classification did not change in 478 (82.1%) and 469 (80.6%) patients for readers 1 and 2, respectively. Twelve (reader 1) or ten (reader 2) patients would not be classified as axSpA if only MRI-SI-s was performed (in the scenario of replacement of mNY), while three (reader 1) or six (reader 2) patients would be additionally classified as axSpA in both scenarios (replacement of mNY and addition of MRI-SI-s). Similar results were seen for the other cut-offs (E≥3, FL≥3). Conclusions Structural lesions on MRI can be used reliably either as an addition to or as a substitute for radiographs in the ASAS axSpA classification of patients in our cohort of patients with short symptom duration.
Complementary and Alternative Medicine: Perceptions of Medical Students from Pakistan

Background: In view of the increasing popularity of complementary and alternative medicine (CAM), it is imperative that medical students, the health professionals of tomorrow, possess adequate knowledge on the topic. Objectives: This is a descriptive study designed to assess the knowledge, attitudes and behavior of medical students about CAM and to capture their perceptions and opinions about its integration into the medical curriculum. Methods: A questionnaire-based cross-sectional survey was done on 198 medical students selected randomly from a Pakistani medical college. Associations between different variables were tested using the χ2-test of significance. Results: Among the 198 participants, a majority believed that some of the CAM modalities are useful; they lacked knowledge, however, about their safety and efficacy. Most of the students believed that it should be used in conjunction with conventional medicine and that, if given adequate training, they would incorporate it in their future medical practice. One-third of the respondents voted in favor of incorporation of CAM into the medical curriculum. Conclusion: Despite being aware of the usefulness of CAM only a few medical students had pursued further knowledge. In order to prepare the medical students of today to better fulfill their duties as tomorrow’s physicians, consideration should be given to incorporating CAM in the medical curriculum.
Efficacy and tolerability of levosulipride, domperidone and metoclopramide in patients with non-ulcer functional dyspepsia: a comparative analysis.

OBJECTIVE This is a prospective, randomized study designed to demonstrate the efficacy of three study drugs (Levosulpiride, Domperidone and Metoclopramide) in Functional Dyspepsia. Subject recruitment was done from medicine outpatient department of the teaching hospital from June 2013 to November 2013. MATERIALS AND METHODS The data collection was performed by the Short-Form Leeds Dyspepsia Questionnaire (SF-LDQ) question instrument for assessing the dyspeptic symptoms of functional dyspepsia in patients enrolled in the study were assessed at base line (Prior to initiation of therapy) and at the next 4 weeks on a 5-point scale. RESULT Among 120 patients 113 patients completed this study in three groups (G-1 Levosulpiride 40 patients, G-2 Domperidone 35 patients and G-3 Metoclopramide 38 patients) were followed up. Female gender was dominated (75), occupation wise most of patients belong to laborer (49) class. Highly significant improvement in symptoms scale was noticed in G-1 Levosulpiride 40 patients' group. CONCLUSION Functional Gastrointestinal disorders are not serious ailments but have a key impact on quality of life. overall dyspeptic symptom relief rates were significantly high in the Levosulpiride group (p<0.004) as compare to Domperidone and Metoclopramide groups. A proper understanding of disease process by health care personnel and by sufferer is obligatory to enhance the quality of life and daunting the self/over the counter medication in this condition.
Analysis of the no-load harmonic electromagnetic field of synchronous generator using tooth flux method

The no-load electromagnetic field of the synchronous generator by the third harmonic excitation is calculated to grasp exactly the match of the third harmonic windings voltage with excitation system, and the third harmonic windings no-load voltage is calculated using tooth flux method, the validation of this method are verified by the comparison of the calculation results and the experiment results on a third harmonic excitation synchronous generator. Based on the method, some main factors that affect the third harmonic no-load electromagnetic field of the synchronous generators by the third harmonic excitation is analyzed, and some useful conclusions, which guide the design for the third harmonic excitation synchronous generators, are gained.
Total Water Intake from Beverages and Foods Is Associated with Energy Intake and Eating Behaviors in Korean Adults

Water is essential for the proper functioning of the body. Even though a recommendation exists for adequate water intake for Koreans, studies identifying actual water intake from all beverages and foods consumed daily in the Korean population are limited. Thus, we estimated total water intake from both beverages and foods and its association with energy intake and eating behaviors in Korean adults. We used a nationally representative sample of 25,122 Korean adults aged ≥19 years, from the Korean National Health and Nutrition Examination Survey 2008–2012. We performed multiple regression analyses, adjusting for sociodemographic and health-related variables to investigate the contribution of overall energy and dietary intakes and eating behaviors to total water intake. The mean total water intake excluding plain water was 1071 g (398 g from beverages and 673 g from foods) and the estimated plain water intake was 1.3 L. Among Korean adults, 82% consumed beverages (excluding plain water) and these beverages contributed to 10% of daily energy intake and 32% of total water intake from beverages and foods. For every 100 kcal/day in energy intake, water intake consumed through beverages and foods increased by 18 g and 31 g, respectively. Water intake from beverages and foods was positively associated with energy from fat and dietary calcium, but inversely associated with energy density and energy from carbohydrates. When there was a 5% increase in energy intake from snacks and eating outside the home, there was an increase in water intake from beverages of 13 g and 2 g, respectively. Increased daily energy intake, the number of eating episodes, and energy intake from snacks and eating outside the home predicted higher water intake from beverages and foods. Our results provide evidence suggesting that various factors, including sociodemographic status, dietary intakes, and eating behaviors, could be important contributors to the water intake of Korean adults. Findings from this cross-sectional analysis may provide insight into strategies for promoting adequate water intake among Koreans.