Miracle-dz/UniFAR-FTUs huggingface dataset:
Genome-wide screen of gamma-secretase–mediated intramembrane cleavage of receptor tyrosine kinases

Receptor tyrosine kinases (RTKs) can signal via regulated intramembrane proteolysis, leading to release of an intracellular receptor fragment with functional activity. A system-wide screen covering 45 of the 55 human RTKs identified 12 novel gamma-secretase substrates and indicated that at least half of all RTKs are susceptible to cleavage.
Physical Fitness and Performance Effect of Intensity of Aerobic Training on V̇O 2 max

GORMLEY, S. E. , D. P. SWAIN, R. HIGH, R. J. SPINA, E. A. DOWLING, U. S. KOTIPALLI, and R. GANDRAKOTA. Effect of Intensity of Aerobic Training on V̇O2max. Med. Sci. Sports Exerc. , Vol. 40, No. 7, pp. 1336–1343, 2008. Purpose: To determine whether various intensities of aerobic training differentially affect aerobic capacity as well as resting HR and resting blood pressure (BP). Methods: Sixty-one health young adult subjects were matched for sex and V̇O2max and were randomly assigned to a moderate(50% V̇O2 reserve (V̇O2R), vigorous (75% V̇O2R), near-maximal-intensity (95% V̇O2R), or a nonexercising control group. Intensity during exercise was controlled by having the subjects maintain target HR based on HR reserve. Exercise volume (and thus energy expenditure) was controlled across the three training groups by varying duration and frequency. Fifty-five subjects completed a 6-wk training protocol on a stationary bicycle ergometer and preand posttesting. During the final 4 wk, the moderate-intensity group exercised for 60 min, 4 dIwk the vigorous-intensity group exercised for 40 min, 4 dIwk and the near-maximal–intensity group exercised 3 dIwk performing 5 min at 75% V̇O2R followed by five intervals of 5 min at 95% V̇O2R and 5 min at 50% V̇O2R. Results: V̇O2max significantly increased in all exercising groups by 7.2, 4.8, and 3.4 mLImin Ikg in the near-maximal–, the vigorous-, and the moderate-intensity groups, respectively. Percent increases in the near-maximal– (20.6%), the vigorous(14.3%), and the moderate-intensity (10.0%) groups were all significantly different from each other (P G 0.05). There were no significant changes in resting HR and BP in any group. Conclusion: When volume of exercise is controlled, higher intensities of exercise are more effective for improving V̇O2max than lower intensities of exercise in healthy, young adults.
The impact of polyphenols on chondrocyte growth and survival: a preliminary report

Background Imbalances in the functional binding of fibroblast growth factors (FGFs) to their receptors (FGFRs) have consequences for cell proliferation and differentiation that in chondrocytes may lead to degraded cartilage. The toxic, proinflammatory, and oxidative response of cytokines and FGFs can be mitigated by dietary polyphenols. Objective We explored the possible effects of polyphenols in the management of osteoarticular diseases using a model based on the transduction of a mutated human FGFR3 (G380R) in murine chondrocytes. This mutation is present in most cases of skeletal dysplasia and is responsible for the overexpression of FGFR3 that, in the presence of its ligand, FGF9, results in toxic effects leading to altered cellular growth. Design Different combinations of dietary polyphenols derived from plant extracts were assayed in FGFR3 (G380R) mutated murine chondrocytes, exploring cell survival, chloride efflux, extracellular matrix (ECM) generation, and grade of activation of mitogen-activated protein kinases. Results Bioactive compounds from Hibiscus sabdariffa reversed the toxic effects of FGF9 and restored normal growth, suggesting a probable translation to clinical requests in humans. Indeed, these compounds activated the intracellular chloride efflux, increased ECM generation, and stimulated cell proliferation. The inhibition of mitogen-activated protein kinase phosphorylation was interpreted as the main mechanism governing these beneficial effects. Conclusions These findings support the rationale behind the encouragement of the development of drugs that repress the overexpression of FGFRs and suggest the dietary incorporation of supplementary nutrients in the management of degraded cartilage.
Data-Driven Cell Zooming for Large-Scale Mobile Networks

In large-scale mobile networks, energy minimization problem pertaining to base station (BS) switching is known to require high computational complexity. In this paper, we propose a data-driven energy-saving (DDE) framework which partitions the networks into communities. Each community can separately make decisions with low computational cost. We introduce a novel metric to measure the collaborative relationship among BSs. Then, the mobile networks are partitioned via a multiscale community detection method. To improve the cell zooming performance, we estimate the aggregate traffic demands of the communities based on the historical traffic profile. A heuristic switch-off strategy is proposed to maximize the energy savings while guaranteeing minimal service requirements. Experiments with two real regional cellular network datasets show that the proposed DDE framework can conserve a significant amount of energy and leverage the tradeoff between energy savings and the blockage ratio.
Multiband and Multimode Concurrent PA With Novel Intermodulation Tuning Network for Linearity Improvement

For multiband power amplifiers (PAs), high impedance at the second-order intermodulation frequencies at the drain can result in severe nonlinear distortion in concurrent mode. To solve this problem, a novel intermodulation tuning network (IMTN) is proposed in this letter. The IMTN is in parallel to the output matching network and has multiple impedance zeros at low frequencies. Therefore, the improvement in linearity is evident even if the frequency difference of two operating bands of PA is relatively large. Experimental measurement is carried out with three sets of dual-band 10-MHz LTE modulated signals, and the carrier frequencies are 1.8/2.1, 2.1/2.6, and 1.8/2.6 GHz, respectively. In mode 2 with 500-MHz frequency difference, the adjacent channel power ratio is improved by 6 dB compared with the conventional PA without IMTN, and in mode 3 with 800-MHz frequency difference, 3 dB is improved compared with low-pass IMTN. The measured average efficiencies of three modes are 40.3%, 36.4% and 37.6%, respectively, at 35-dBm total output power.
The Unpredictable Effect of Changing Cardiac Output on Hypoxemia after Acute Pulmonary Thromboembolism

Previous studies reported that the degree of hypoxemia following acute pulmonary thromboembolism (APTE) was highly variable and that its mechanism was mainly due to the creation of many high and low ventilation/perfusion (V/Q) units, as a result of the heterogeneous regional blood flow (Q) caused by embolic obstruction. We studied the effect of changing cardiac output (Qt) on gas exchange after APTE in 5 embolized piglets (23 ± 3 Kg), using Dobutamine intermittently at approximately 20 μg/kg/min for 120 minutes. The distribution of ventilation (V) and perfusion (Q) at various times was mapped using fluorescent microspheres in 941 ± 60 lung regions. After APTE, increase in Qt by Dobutamine improved venous oxygen tension (PvO2) but arterial PaO2 did not change consistently. On the other hand, cluster analysis showed that the V/Q ratio of most lung regions was lowered due to increases in Q at the same time. We concluded that the effect of changing cardiac output on gas exchange following APTE was affected by the simultaneous and varying balance between the changing V/Q mismatch and the concomitantly changing PvO2, which might explain the unpredictability of PaO2 in the clinical setting.
A 23.6Mb/mm2 SRAM in 10nm FinFET technology with pulsed PMOS TVC and stepped-WL for low-voltage applications

The emergence of cloud computing and big data analytics, accompanied by a sustained growth of battery-powered mobile devices, continues to drive the importance of energy and area efficient CPU and SoC designs. Low-voltage operation remains one of the primary approaches for active power reduction, but SRAM l/MIN can limit the minimum operating voltage. Device size quantization continues to be a challenge for compact 6T SRAM design in FinFET technologies, where careful co-optimization of the technology and assist circuit design is required for high-density low-voltage array implementations. This paper presents two SRAM array designs in a 10nm low-power CMOS technology featuring 3rd generation FinFET transistors: a high-density 23.6Mb/mm2 array and a low-voltage 20.4Mb/mm2 array.
SPoMo: IMU-based real-time sitting posture monitoring

Improper sitting posture can lead to a number of serious health disorders connected to the musculoskeletal system. Especially nowadays sitting periods at work or at home are increasing, and therefore consistent stance monitoring can help users improve their sitting habits and avoid related complications. In this work, we present SPoMo a real-time, practical, wearable system that automatically tracks the user's sitting posture, through wireless sensors attached to his back. Our prototype is based on Inertial Measurement Units (IMUs) that monitor the angle deviation from the optimal position. We have also developed a cloud-assisted mobile application able to continuously visualize the user's stance, archive his performance over time and provide insight for future development of proper posture. We focus on presenting SPoMo functionality and experimentally evaluate its performance. Our results prove our system to be accurate and therefore a good every-day solution that helps the consumer to avoid chronic improper posture.
Relationship between ethical leadership and organisational commitment of nurses with perception of patient safety culture

AIM To determine the relationship between ethical leadership, organisational commitment of nurses and their perception of patient safety culture. BACKGROUND Patient safety, organisational commitment and ethical leadership styles are very important for improving the quality of nursing care. METHODS In this descriptive-correlational study, 340 nurses were selected using random sampling from the hospitals in Tehran in 2016. Data were analysed using descriptive and inferential statistics in SPSS v.20. RESULTS There was a significant positive relationship between the ethical leadership of nursing managers, perception of patient safety culture and organisational commitment. The regression analysis showed that nursing managers' ethical leadership and nurses' organisational commitment is a predictor of patient safety culture and confirms the relationship between the variables. CONCLUSION Regarding the relationship between the nurses' safety performance, ethical leadership and organisational commitment, it seems that the optimisation of the organisational commitment and adherence to ethical leadership by administrators and managers in hospitals could improve the nurses' performance in terms of patient safety. IMPLICATIONS FOR NURSING MANAGEMENT Implementing ethical leadership seems to be one feasible strategy to improve nurses' organisational commitment and perception of patient safety culture. Efforts by nurse managers to develop ethical leadership reinforce organisational commitment to improve patient outcomes. Nurse managers' engagement and performance in this process is vital for a successful result.
Structure of HsdS Subunit from Thermoanaerobacter tengcongensis Sheds Lights on Mechanism of Dynamic Opening and Closing of Type I Methyltransferase

Type I DNA methyltransferases contain one specificity subunit (HsdS) and two modification subunits (HsdM). The electron microscopy model of M.EcoKI-M2S1 methyltransferase shows a reasonable closed state of this clamp-like enzyme, but the structure of the open state is still unclear. The 1.95 Å crystal structure of the specificity subunit from Thermoanaerobacter tengcongensis (TTE-HsdS) shows an unreported open form inter-domain orientation of this subunit. Based on the crystal structure of TTE-HsdS and the closed state model of M.EcoKI-M2S1, we constructed a potential open state model of type I methyltransferase. Mutational studies indicated that two α-helices (aa30-59 and aa466-495) of the TTE-HsdM subunit are important inter-subunit interaction sites in the TTE-M2S1 complex. DNA binding assays also highlighted the importance of the C-terminal region of TTE-HsdM for DNA binding by the TTE-M2S1 complex. On the basis of structural analysis, biochemical experiments and previous studies, we propose a dynamic opening and closing mechanism for type I methyltransferase.
Destabilization of the replication fork protection complex disrupts meiotic chromosome segregation

Loss of the FPC protein Swi1 or Swi3 results in reduced spore viability, delayed replication, changes in recombination, reduced centromeric cohesion stability, and chromosome missegregation in meiosis I and II. These phenotypes reveal a crucial link between meiotic replication fork stability and chromosome segregation.
Elicitation of Both Anti HIV-1 Env Humoral and Cellular Immunities by Replicating Vaccinia Prime Sendai Virus Boost Regimen and Boosting by CD40Lm

For protection from HIV-1 infection, a vaccine should elicit both humoral and cell-mediated immune responses. A novel vaccine regimen and adjuvant that induce high levels of HIV-1 Env-specific T cell and antibody (Ab) responses was developed in this study. The prime-boost regimen that used combinations of replication-competent vaccinia LC16m8Δ (m8Δ) and Sendai virus (SeV) vectors expressing HIV-1 Env efficiently produced both Env-specific CD8+ T cells and anti-Env antibodies, including neutralizing antibodies (nAbs). These results sharply contrast with vaccine regimens that prime with an Env expressing plasmid and boost with the m8Δ or SeV vector that mainly elicited cellular immunities. Moreover, co-priming with combinations of m8Δs expressing Env or a membrane-bound human CD40 ligand mutant (CD40Lm) enhanced Env-specific CD8+ T cell production, but not anti-Env antibody production. In contrast, priming with an m8Δ that coexpresses CD40Lm and Env elicited more anti-Env Abs with higher avidity, but did not promote T cell responses. These results suggest that the m8Δ prime/SeV boost regimen in conjunction with CD40Lm expression could be used as an immunization platform for driving both potent cellular and humoral immunities against pathogens such as HIV-1.
Effects of particle size distribution of cut tobacco on cigarette weight and density

Grey incidence was employed to analyze relationship between PSD and cigarette weight and density.Results showed that: within certain range,the effects of cut tobacco longer than 3.35 mm on weight and density and on their stability were opposite.As the proportion of cut tobacco longer than 3.35 mm increased,weight and density decreased and hence reduced stability.The effect of different size of cut tobacco on weight and density was different.Weight and density was less affected by cut tobacco longer than 5.60 mm but mostly affected by cut tobacco between 2.80 mm and 4.75 mm.
Pilot Power Allocation Through User Grouping in Multi-Cell Massive MIMO Systems

In this paper, we propose a relative channel estimation error (RCEE) metric, and derive closed-form expressions for its expectation <inline-formula> <tex-math notation="LaTeX">$\rm {Exp}_{rcee}$ </tex-math></inline-formula> and the achievable uplink rate holding for any number of base station antennas <inline-formula> <tex-math notation="LaTeX">$M$ </tex-math></inline-formula>, with the least squares (LS) and minimum mean squared error (MMSE) methods. It is found that RCEE and <inline-formula> <tex-math notation="LaTeX">$\rm {Exp}_{rcee}$ </tex-math></inline-formula> converge to the same constant value when <inline-formula> <tex-math notation="LaTeX">$M\rightarrow \infty $ </tex-math></inline-formula>, which renders the pilot power allocation (PPA) substantially simplified and a PPA algorithm is proposed to minimize the average <inline-formula> <tex-math notation="LaTeX">$\rm {Exp}_{rcee}$ </tex-math></inline-formula> per user under a total pilot power budget <inline-formula> <tex-math notation="LaTeX">$P$ </tex-math></inline-formula> in multi-cell massive multiple-input multiple-output systems. Numerical results show that the PPA algorithm brings considerable gains for the LS estimation compared with equal PPA (EPPA), while the gains are significant only with large frequency reuse factor (FRF) for the MMSE estimation. Moreover, for large FRF and large <inline-formula> <tex-math notation="LaTeX">$P$ </tex-math></inline-formula>, the performance of the LS approaches to that of the MMSE. Besides, a scheduling strategy is proposed to allocate pilot power in the whole system, which can approach the optimal performance. For the achievable uplink rate, the PPA scheme and improves the minimum achievable uplink rate compared with the EPPA scheme.
Attention Demands of Spoken Word Planning: A Review

Attention and language are among the most intensively researched abilities in the cognitive neurosciences, but the relation between these abilities has largely been neglected. There is increasing evidence, however, that linguistic processes, such as those underlying the planning of words, cannot proceed without paying some form of attention. Here, we review evidence that word planning requires some but not full attention. The evidence comes from chronometric studies of word planning in picture naming and word reading under divided attention conditions. It is generally assumed that the central attention demands of a process are indexed by the extent that the process delays the performance of a concurrent unrelated task. The studies measured the speed and accuracy of linguistic and non-linguistic responding as well as eye gaze durations reflecting the allocation of attention. First, empirical evidence indicates that in several task situations, processes up to and including phonological encoding in word planning delay, or are delayed by, the performance of concurrent unrelated non-linguistic tasks. These findings suggest that word planning requires central attention. Second, empirical evidence indicates that conflicts in word planning may be resolved while concurrently performing an unrelated non-linguistic task, making a task decision, or making a go/no-go decision. These findings suggest that word planning does not require full central attention. We outline a computationally implemented theory of attention and word planning, and describe at various points the outcomes of computer simulations that demonstrate the utility of the theory in accounting for the key findings. Finally, we indicate how attention deficits may contribute to impaired language performance, such as in individuals with specific language impairment.
Variation in the Male Pheromones and Mating Success of Wild Caught Drosophila melanogaster

Drosophila melanogaster males express two primary cuticular hydrocarbons (male-predominant hydrocarbons). These act as sex pheromones by influencing female receptivity to mating. The relative quantities of these hydrocarbons vary widely among natural populations and can contribute to variation in mating success. We tested four isofemale lines collected from a wild population to assess the effect of intrapopulation variation in male-predominant hydrocarbons on mating success. The receptivity of laboratory females to males of the four wild-caught lines varied significantly, but not consistently in the direction predicted by variation in male-predominant hydrocarbons. Receptivity of the wild-caught females to laboratory males also varied significantly, but females from lines with male-predominant hydrocarbon profiles closer to a more cosmopolitan one did not show a correspondingly strong mating bias toward a cosmopolitan male. Among wild-caught lines, the male-specific ejaculatory bulb lipid, cis-vaccenyl acetate, varied more than two-fold, but was not associated with variation in male mating success. We observed a strong inverse relationship between the receptivity of wild-caught females and the mating success of males from their own lines, when tested with laboratory flies of the opposite sex.
Allosteric dynamics of SAMHD 1 studied by molecular dynamics simulations

SAMHD1 is a human cellular enzyme that blocks HIV-1 infection in myeloid cells and non-cycling CD4+T cells. The enzyme is an allosterically regulated triphosphohydrolase that modulates the level of cellular dNTP. The virus restriction is attributed to the lowering of the pool of dNTP in the cell to a point where reverse-transcription is impaired. Mutations in SAMHD1 are also implicated in Aicardi-Goutières syndrome. A mechanistic understanding of the allosteric activation of the enzyme is still elusive. We have performed molecular dynamics simulations to examine the allosteric site dynamics of the protein and to examine the connection between the stability of the tetrameric complex and the Allosite occupancy.
Water Browning Influences the Behavioral Effects of Ultraviolet Radiation on Zooplankton

In the last decades, limnic water bodies in the Northern hemisphere have experienced a noticeable browning, i.e., increasing levels of dissolved organic matter (DOM). While the effects on primary producers is usually considered negative (light attenuation), zooplankton is thought to benefit from increased DOM, which absorbs harmful ultraviolet radiation (UVR). However, behavioural alterations due to browning in zooplankton have not yet been studied. We investigated the effects of a DOM gradient, alone and in combination with UVR, on the swimming behaviour of Daphnia magna. Making use of a computer-controlled imaging system, we repeatedly filmed individuals over six hours and analysed the video material to unravel effects on exploration behaviour and other motility patterns. The results show that increasing DOM buffers the detrimental effects of UVR on swimming behaviour. This is likely due to attenuation of UVR by DOM. Interestingly, DOM also raised the overall swimming activity independent of UVR exposure. Our findings highlight the importance of DOM in freshwater systems, not only because of its physico-chemical properties, but also due to its higher-level effects on zooplankton communities.
A New Extensively Characterised Conditionally Immortal Muscle Cell-Line for Investigating Therapeutic Strategies in Muscular Dystrophies

A new conditionally immortal satellite cell-derived cell-line, H2K 2B4, was generated from the H2Kb-tsA58 immortomouse. Under permissive conditions H2K 2B4 cells terminally differentiate in vitro to form uniform myotubes with a myogenic protein profile comparable with freshly isolated satellite cells. Following engraftment into immunodeficient dystrophin-deficient mice, H2K 2B4 cells regenerated host muscle with donor derived myofibres that persisted for at least 24 weeks, without forming tumours. These cells were readily transfectable using both retrovirus and the non-viral transfection methods and importantly upon transplantation, were able to reconstitute the satellite cell niche with functional donor derived satellite cells. Finally using the Class II DNA transposon, Sleeping Beauty, we successfully integrated a reporter plasmid into the genome of H2K 2B4 cells without hindering the myogenic differentiation. Overall, these data suggest that H2K 2B4 cells represent a readily transfectable stable cell-line in which to investigate future stem cell based therapies for muscle disease.