Miracle-dz/UniFAR-FTUs huggingface dataset:
An Atypical PKC Directly Associates and Colocalizes at the Epithelial Tight Junction with ASIP, a Mammalian Homologue of Caenorhabditis elegans Polarity Protein PAR-3

Cell polarity is fundamental to differentiation and function of most cells. Studies in mammalian epithelial cells have revealed that the establishment and maintenance of cell polarity depends upon cell adhesion, signaling networks, the cytoskeleton, and protein transport. Atypical protein kinase C (PKC) isotypes PKCζ and PKCλ have been implicated in signaling through lipid metabolites including phosphatidylinositol 3-phosphates, but their physiological role remains elusive. In the present study we report the identification of a protein, ASIP (atypical PKC isotype–specific interacting protein), that binds to aPKCs, and show that it colocalizes with PKCλ to the cell junctional complex in cultured epithelial MDCKII cells and rat intestinal epithelia. In addition, immunoelectron microscopy revealed that ASIP localizes to tight junctions in intestinal epithelial cells. Furthermore, ASIP shows significant sequence similarity to Caenorhabditis elegans PAR-3. PAR-3 protein is localized to the anterior periphery of the one-cell embryo, and is required for the establishment of cell polarity in early embryos. ASIP and PAR-3 share three PDZ domains, and can both bind to aPKCs. Taken together, our results suggest a role for a protein complex containing ASIP and aPKC in the establishment and/or maintenance of epithelial cell polarity. The evolutionary conservation of the protein complex and its asymmetric distribution in polarized cells from worm embryo to mammalian-differentiated cells may mean that the complex functions generally in the organization of cellular asymmetry.
Review on latent fingerprint matching techniques

The best form of identifying a person for criminal investigation is from the fingerprint. Identifying suspects based on latent fingerprint is a procedure that is extremely important to forensics and law enforcement agencies. The small number of minutiae and the noise characteristic of latents make it extremely difficult to automatically match latents to their mated full prints that are stored in law enforcement databases. The various latent enhancement techniques and matching algorithms are discussed in this paper.
Identification of a Novel Calcium Binding Motif Based on the Detection of Sequence Insertions in the Animal Peroxidase Domain of Bacterial Proteins

Proteins of the animal heme peroxidase (ANP) superfamily differ greatly in size since they have either one or two catalytic domains that match profile PS50292. The orf PP_2561 of Pseudomonas putida KT2440 that we have called PepA encodes a two-domain ANP. The alignment of these domains with those of PepA homologues revealed a variable number of insertions with the consensus G-x-D-G-x-x-[GN]-[TN]-x-D-D. This motif has also been detected in the structure of pseudopilin (pdb 3G20), where it was found to be involved in Ca2+ coordination although a sequence analysis did not reveal the presence of any known calcium binding motifs in this protein. Isothermal titration calorimetry revealed that a peptide containing this consensus motif bound specifically calcium ions with affinities ranging between 33–79 µM depending on the pH. Microcalorimetric titrations of the purified N-terminal ANP-like domain of PepA revealed Ca2+ binding with a KD of 12 µM and stoichiometry of 1.25 calcium ions per protein monomer. This domain exhibited peroxidase activity after its reconstitution with heme. These data led to the definition of a novel calcium binding motif that we have termed PERCAL and which was abundantly present in animal peroxidase-like domains of bacterial proteins. Bacterial heme peroxidases thus possess two different types of calcium binding motifs, namely PERCAL and the related hemolysin type calcium binding motif, with the latter being located outside the catalytic domains and in their C-terminal end. A phylogenetic tree of ANP-like catalytic domains of bacterial proteins with PERCAL motifs, including single domain peroxidases, was divided into two major clusters, representing domains with and without PERCAL motif containing insertions. We have verified that the recently reported classification of bacterial heme peroxidases in two families (cd09819 and cd09821) is unrelated to these insertions. Sequences matching PERCAL were detected in all kingdoms of life.
Resource Dependencies of Old Age Homes

Abstract This article presents an approach to operationalizing and measuring the theoretical concept of “resource dependency” of organizations, based on the Resource Dependency Theory, which views organizations as dependent upon resources they can enlist from their environments. A measure that quantifies organizational dependency, which was carried out within forty-seven old age homes in Israel, was developed. The measure includes seven dimensions; degree of resource control, number of sources, types of sources, degrees of resource essentiality, degree of certainty, impact of resource reduction or cessation, and availability of alternative sources. Measuring and scoring procedures are introduced for three different types of resources: revenues, clients, and services. Methodological aspects of the procedures are discussed and implications for practice and further research are proposed.
The ICSI Summarization System at TAC 2008

The ICSI multi-document summarization system relies on a general framework that casts summarization as a global optimization problem with an integer linear programming solution. Our primary submission, a simple sentence extractor with an n-gram frequency heuristic, gives results at least as good as any reported on the non-update part of the main task. Our secondary submission adds compressed sentence alternatives, achieving high ROUGE scores but lower manual scores. We also observe that an oracle version of our sentence extractor is nearly a direct optimization of ROUGE. We show oracle results for the TAC data set and discuss their significance. Finally, we provide a detailed analysis of the linguistic quality of our two systems, suggesting specifically where improvements might be most useful.
Real-time Geometry and Reflectance Capture for Digital Face Replacement

In this work, we develop a real-time geometry capture approach to digital face replacement for a dynamic performance. Digital face replacement has major applications in visual effects for motion pictures as well as interactive applications such as video games and simulation and training environments. Our approach looks into extending the 3D face scanning technology developed at the ICT Graphics Lab [Ma 2008] to support seamless face replacement along with separated diffuse and specular albedo textures and surface normals for high quality post-production relighting of the captured performance (see Figure 1). Such an approach goes beyond the traditional scope of face replacement techniques that are either completely image based and hence viewdependent or typically capture a performance under a fixed lighting condition and hence cannot be relit for usage in other performances [Zhang and Yau 2006].
A Modified FBLMS Algorithm Research and Performance Analysis

As there is a conflict about block step size between the requirements of the convergence velocity and parameters' maladjustment within FBLMS algorithm, a modified FBLMS algorithm is well presented in this paper which uses a variable block step size to solve the contradiction between convergence rate and precision. The modified FBLMS algorithm is simulated in the MATLAB platform. The simulation results prove that this algorithm not only has a faster convergence speed, a faster tracking speed and a smaller stationary error, but also has a better anti-jamming capability.
Editorial: Detailed guidelines for reporting quantitative research in Health & Social Care in the Community

As Statistical Editor of Health & Social Care in the Community (HSCC), I statistically and editorially review all manuscripts reporting empirical quantitative research before they are accepted for publication. Some common faults in statistical analysis occur from time to time, but the problem that causes most work in statistical review lies in the reporting. By expanding our author guidelines for reporting quantitative research studies in HSCC, this article aims to help prospective authors with the planning and writing of their manuscripts and facilitate the peer review process. It gives a more detailed explanation of the structure, layout and content expected for manuscripts reporting the results of empirical quantitative research. The article includes general advice on the reporting of statistical findings, outlines common statistical faults seen in submitted manuscripts, and covers the reporting of commonly-used methods with examples of good practice from HSCC and suggested layouts for tables. Guidance on how to submit a paper to HSCC is also included. Authors intending to submit quantitative manuscripts to HSCC are strongly encouraged to read our author guidelines and this article to help them structure, write and submit their manuscripts. While this article goes into considerable detail, prospective authors may only need to study relevant sections to improve their manuscripts.
ACRE advice : New techniques used in plant breeding

ACRE has discussed these techniques in the context of plant breeding although many of them (or equivalents) are used in the modification of other organisms such as animals and microorganisms. We have come to our conclusions about the status of these techniques and the organisms that they generate by determining whether the scientific terms in the definition apply in each case. However, as the legal significance of many of these terms is not clear, Ministers will need to seek a legal opinion on our conclusions. Our considerations have highlighted three main issues where legal clarification is required. These issues are as follows.
Exact solution of quasi two and three dimensional quantum spin models

A class of quasi two and three dimensional quantum lattice spin models with nearest and next nearest neighbour interactions is proposed. The basic idea of construction is to introduce interactions in an array of XXZ spin chains through twisting transformation. The models belong to quantum integrable systems allowing explicit R-matrix solution. The eigenvalue problem can be solved exactly using some symmetry of the models.
A NEW APPROACH TO ANALYSIS OF INDUCTION MOTORS WITH ROTOR FAULTS DURING STARTUP BASED ON THE FINITE ELEMENT METHOD

The increasing popularity of a so-called transient motor current signature analysis requires the fault diagnostics parameters which could not be exposed to other factors irrelevant to the fault to make a precise assessment of the failure severity level. This challenging task needs a precise modeling of faulty motor behavior in various operating conditions at difierent fault severity levels. This paper introduces a new approach to the flnite element analysis of an induction motor with broken rotor bars during startup. The approach is based on the principle of superposition and contributes to examination of the fault rotor backward rotating magnetic fleld and current components produced by such fleld separating them from stator currents. It gives a new sight on the behavior of a faulty motor during startup for the diagnosis purposes. Further analysis of the simulation data by means of the Extended Park's Vector Approach and the continuous wavelet transform and its experimental validation is also presented in the paper.
Nuclear norm regularized robust dictionary learning for energy disaggregation

The goal of this work is energy disaggregation. A recent work showed that instead of employing the usual Euclidean norm cost function for dictionary learning, better results can be achieved by learning the dictionaries in a robust fashion by employing an l1-norm cost function; this is because energy data is corrupted by large but sparse outliers. In this work we propose to improve the robust dictionary learning approach by imposing low-rank penalty on the learned coefficients. The ensuing formulation is solved using a combination of Split Bregman and Majorization Minimization approach. Experiments on the REDD dataset reveal that our proposed method yields better results than both the robust dictionary learning technique and the recently published work on powerlet energy disaggregation.
Design of a Parallel Hybrid Direct/Iterative Solver for CFD Problems

We discuss the parallel implementation of a hybrid direct/iterative solver for a special class of saddle point matrices arising from the discretization of the steady Navier-Stokes equations on an Arakawa C-grid, the F-matrices. The two-level method described here has the following properties: (i) it is very robust, even at comparatively high Reynolds Numbers, (ii) a single parameter controls fill and convergence, making the method straightforward to use, (iii) the convergence rate is independent of the number of unknowns, (iv) it can be implemented on distributed memory machines in a natural way, (v) the matrix on the second level has the same structure and numerical properties as the original problem, so the method can be applied recursively. The implementation focusses on generality, modularity, code reuse and recursiveness. The solver is implemented using building blocks of the Trilinos libraries. We show its performance on a parallel computer for the Navier-Stokes equations.
Specification-based Retrieval of Software Components through Fuzzy Inference

In the component based software engineering approach, a software system is viewed as an assembly of reusable independently developed components. In order to produce automated tools to support the selection and assembly of components, rigorous specifications of components and performant retrieval and selection strategies based on these specifications are needed. Classical approaches for automatically retrieving components that match a set of required properties result mostly in very complex solutions. In this article we propose an efficient fuzzy logic based solution for the specification and retrieval of software components. We describe the basic principles of the proposed solutions and illustrate them on example scenarious.
Phenylpropanoids Accumulation in Eggplant Fruit: Characterization of Biosynthetic Genes and Regulation by a MYB Transcription Factor

Phenylpropanoids are major secondary metabolites in eggplant (Solanum melongena) fruits. Chlorogenic acid (CGA) accounts for 70–90% of total phenolics in flesh tissues, while anthocyanins are mainly present in the fruit skin. As a contribution to the understanding of the peculiar accumulation of these health-promoting metabolites in eggplant, we report on metabolite abundance, regulation of CGA and anthocyanin biosynthesis, and characterization of candidate CGA biosynthetic genes in S. melongena. Higher contents of CGA, Delphinidin 3-rutinoside, and rutin were found in eggplant fruits compared to other tissues, associated to an elevated transcript abundance of structural genes such as PAL, HQT, DFR, and ANS, suggesting that active in situ biosynthesis contributes to anthocyanin and CGA accumulation in fruit tissues. Putative orthologs of the two CGA biosynthetic genes PAL and HQT, as well as a variant of a MYB1 transcription factor showing identity with group six MYBs, were isolated from an Occidental S. melongena traditional variety and demonstrated to differ from published sequences from Asiatic varieties. In silico analysis of the isolated SmPAL1, SmHQT1, SmANS, and SmMyb1 promoters revealed the presence of several Myb regulatory elements for the biosynthetic genes and unique elements for the TF, suggesting its involvement in other physiological roles beside phenylpropanoid biosynthesis regulation. Transient overexpression in Nicotiana benthamiana leaves of SmMyb1 and of a C-terminal SmMyb1 truncated form (SmMyb1Δ9) resulted in anthocyanin accumulation only of SmMyb1 agro-infiltrated leaves. A yeast two-hybrid assay confirmed the interaction of both SmMyb1 and SmMyb1Δ9 with an anthocyanin-related potato bHLH1 TF. Interestingly, a doubled amount of CGA was detected in both SmMyb1 and SmMyb1Δ9 agro-infiltrated leaves, thus suggesting that the N-terminal region of SmMyb1 is sufficient to activate its synthesis. These data suggest that a deletion of the C-terminal region of SmMyb1 does not limit its capability to regulate CGA accumulation, but impairs anthocyanin biosynthesis. To our knowledge, this is the first study reporting a functional elucidation of the role of the C-term conserved domain in MYB activator proteins.
Renal handling of phenol red. III. Bidirectional transport.

1. The renal excretion of phenol red and other phenolsulphophthalein dyes (bromophenol blue and bromothymol blue) was studied in clearance experiments on anaesthetized rabbits. 2. Net tubular excretion of phenol red reached a maximal value of 8 mumole/min at a plasma concentration of ultrafiltrable dye of about 0.1 mM and was decreased at higher plasma concentrations. Decreases in net tubular excretion at high plasma concentrations were also obtained for bromophenol blue and bromothymol blue suggesting tubular reabsorption in addition to tubular secretion of the dye. Conclusive evidence for reabsorption was provided by administration of probenecid which caused a fall in the excretion of the dyes below that filtered by the glomeruli. 3. Tubular reabsorption of phenol red during probenecid administration appeared to be proportional to the glomerular load and was increased under experimental conditions leading to a decrease of urinary pH. Experiments involving efflux of phenol red from liposomes gave no evidence of a significant role of transmembrane passage by non‐ionic diffusion. It is suggested that the pH dependence of the reabsorptive process is the result of preferential reabsorption of the acid as compared to the basic form of the indicator dye across a hydrophilic pathway in the transporting membranes. 4. Clearance ratio of phenol red to that of p‐aminohippurate at low plasma concentrations was about 0.3. They low degree of extraction of phenol red from renal plasma is attributed both to tubular reabsorption and binding of the dye by plasma proteins.
Hardware-enforced Comprehensive Memory Safety the Watchdog Approach Efficiently Eliminates an Entire Class of Security Vulnerabilities by Enforcing Memory Safety in Hardware. Watchdog Maintains Per-pointer Bounds and Identifier Metadata in a Disjoint Shadow Space to Ensure Compatibility with Existin

Low-level systems software— such as operating systems, virtual machines, language runtimes, embedded software, and performance-critical applications—is commonly written in unsafe languages, notably C and C++. These low-level languages remain prevalent because they provide high performance, direct access to the underlying hardware, and explicit control over memory management. Moreover, because such systems often consist of millions of lines of code, transitioning the computing ecosystem away from C and its variants is not feasible any time soon. Unfortunately, C and its variants do not enforce memory safety. Informally, memory safety requires that all memory accesses performed by a program adhere to the language specification (that is, all accesses refer only to allocated memory within the prescribed object bounds). Violations of memory safety arise in two ways. First, a spatial memory safety violation, or buffer overflow, occurs when a program accesses a memory location outside of the allocated region of an object or array. Second, a temporal memory safety violation (also called a dangling pointer dereference or use-after-free error) occurs when a program accesses a memory location that has already been deallocated. See Figure 1 for examples of spatial and temporal safety violations. Without memory safety, seemingly benign program bugs anywhere in the code base can cause silent memory corruption, difficultto-diagnose crashes, and incorrect results. Worse yet, lack of memory safety enforcement is the root cause of a multitude of security vulnerabilities because it allows attackers to exploit a memory safety error to corrupt program execution by giving the vulnerable program a suitably crafted input. Buffer overflows, use-after-free errors, and other lowlevel vulnerabilities stemming from the lack of memory safety compromise the security of the computing ecosystem as a whole. Over the last few years, we’ve embarked on a research project focused on bringing memory safety enforcement to C and C++. The goal is to retrofit unmodified C and C++ code with memory safety so that the code is as safe as code written in [3B2-9] mmi2013030038.3d 17/5/013 17:45 Page 38
The application of online surveys for workplace health research

Work has a synergistic relationship to health, and workplaces are increasingly the focus for both health promotion initiatives and health research.1 Electronic technologies are a common feature of many workplaces and as such are convenient low cost survey vehicles for the health researcher. The methods and the implications of employing Internet methods for health research are covered and a wide range of issues connected with online sampling and data collection are discussed. Because the workplace does not provide a neutral research environment, the security, anonymity and associated issues of using the two main forms of Internet surveys for workplace health research are examined and their advantages and disadvantages are debated.
Tetraspanin CO-029 Inhibits Colorectal Cancer Cell Movement by Deregulating Cell-Matrix and Cell-Cell Adhesions

Alterations in tetraspanin CO-029 expression are associated with the progression and metastasis of cancers in the digestive system. However, how CO-029 promotes cancer metastasis is still poorly understood. To determine the mechanism, we silenced CO-029 expression in HT29 colon cancer cells and found that the CO-029 knockdown significantly reduced cell migratory ability. The diminished cell migration was accompanied by the upregulation of both integrin-dependent cell-matrix adhesion on laminin and calcium-dependent cell-cell adhesion. The cell surface levels of laminin-binding integrin α3β1 and fibronectin-integrin α5β1 were increased while the level of CD44 was decreased upon CO-029 silencing. These changes contribute to the altered cell-matrix adhesion. The deregulated cell-cell adhesion results, at least partially, from increased activity of cadherins and reduced level of MelCAM. In conclusion, CO-029 functions as a regulator of both cell-matrix and cell-cell adhesion. During colon cancer progression, CO-029 promotes cancer cell movement by deregulating cell adhesions.
Synchronization in Social Tasks: Robotic Drumming

Music performance is an important, well-structured setting for evaluating a robot's ability to detect, understand and respond appropriately to complex human activity. Social tasks such as cooperative performance require participants to detect, interpret and attune to the actions of their partners quickly and accurately. The synthesis of multiple sensory perceptions may be a fruitful approach to this problem. In order to evaluate this approach, we programmed a humanoid robot, Nico, to play a drum in concert with human drummers and at the direction of a human conductor. Our results show that sensory integration can enable precise synchronization in social tasks even when perceptual data is imperfect, misleading and subject to extensive processing delay. By integrating several streams of information - visual, auditory, and proprioceptive - Nico can attune to a tempo that is set by a human conductor, in concert with human performers. Nico continuously evaluates its perceptions of its own actions and those of the humans around it, dealing with unforeseen changes in tempo and affect in real time