Miracle-dz/UniFAR-FTUs huggingface dataset:
The Role of Intersubunit Interactions for the Stabilization of the T State of Escherichia coli Aspartate Transcarbamoylase*

Homotropic cooperativity in Escherichia coli aspartate transcarbamoylase results from the substrate-induced transition from the T to the R state. These two alternate states are stabilized by a series of interdomain and intersubunit interactions. The salt link between Lys-143 of the regulatory chain and Asp-236 of the catalytic chain is only observed in the T state. When Asp-236 is replaced by alanine the resulting enzyme exhibits full activity, enhanced affinity for aspartate, no cooperativity, and no heterotropic interactions. These characteristics are consistent with an enzyme locked in the functional R state. Using small angle x-ray scattering, the structural consequences of the D236A mutant were characterized. The unliganded D236A holoenzyme appears to be in a new structural state that is neither T, R, nor a mixture of T and R states. The structure of the native D236A holoenzyme is similar to that previously reported for another mutant holoenzyme (E239Q) that also lacks intersubunit interactions. A hybrid version of aspartate transcarbamoylase in which one catalytic subunit was wild-type and the other had the D236A mutation was also investigated. The hybrid holoenzyme, with three of the six possible interactions involving Asp-236, exhibited homotropic cooperativity, and heterotropic interactions consistent with an enzyme with both T and R functional states. Small angle x-ray scattering analysis of the unligated hybrid indicated that the enzyme was in a new structural state more similar to the T than to the R state of the wild-type enzyme. These data suggest that three of the six intersubunit interactions involving D236A are sufficient to stabilize a T-like state of the enzyme and allow for an allosteric transition.
Register based on efficient scene learning and keypoint matching for Augmented Reality system

Register is steadily gaining in importance due to the drive from various computer vision applications, such as Augmented Reality (AR), mobile computing, and human-machine interface. Efficient keypoint-based approachs are widely used in scene register. This paper focuses on designing a robust and flexible registration method for wide-area augmented reality applications using scene recognition and natural features tracking techniques. A linear structured SVM classifier is used to perform scene learning online, which allows us to quickly adapt our model to a given environment. And the hybrid tracking strategy is implemented by combining both wide and narrow baseline techniques. The experiments have been conducted to demonstrate the validity of our methods.
Combining genetic algorithm and pairwise testing for optimised test generation from UML ADs

Unified modelling language (UML) activity diagram (AD) is used to represent system behaviour abstractly and is used by testers to generate test cases and test data. During the design of test cases, AD with concurrent activities may lead to a large number of paths, and it may not always be possible to test all execution paths. Research on deriving test cases from AD having concurrent activities has focused on conventional search techniques such as breadth-first search and depth-first search which has been found to be inefficient in such cases. To overcome this drawback, the authors propose a method using pairwise testing and genetic algorithm to derive a reduced number of test cases in AD with concurrent activities. Experiments conducted on various real-life concurrent systems show that the proposed technique generates a reduced number of test cases compared with existing methods.
HTSFilter : Data-based filtering for repli- cated transcriptome sequencing experiments

This vignette illustrates the use of the HTSFilter package to filter replicated data from transcriptome sequencing experiments (e.g., RNA sequencing data) for a variety of different data classes: matrix, data.frame, the S3 classes associated with the edgeR package (DGEExact and DGELRT ), and the S4 class associated with the DESeq2 package (DESeqDataSet).
tert-Butyl 1-hydroxypiperidine-2-carboxylate

The title compound, C10H19NO3, is a disubstituted piperidine bearing substituents in two equatorial positions. One of the substituents is a hydroxy group bound to nitrogen and the second a tert-butyl ester group bound to the carbon next to the endocyclic nitrogen. Enantiomers of the title compound form hydrogen-bridged dimers across a center of inversion.
Malware Detection Techniques in Android

Phones have become an important need of today. The term mobile phone and smart phone are almost identical now - a-days. Smartphone market is booming with very high speed. Smartphones have gained such a huge popularity due to wide range of capabilities they offer. Currently android platform is leading the smartphone market. Android has gained an overnight popularity and became the top OS among its competitor OS. This eminence attracted malware authors as well. As android is an open source platform, it seems quite easy for malware authors to fulfill their illicit intentions. In this paper a new technique will be introduced to detect malware. This technique detects malware in android applications through machine learning classifier by using both static and dynamic analysis. This technique does not rely on malware signatures for static analysis but instead android permission model is used. Under dynamic analysis, system call tracing is performed. Using both static and dynamic techniques along with machine learning provides all in one solution for malware detection. The technique used by us is tested on various benign samples collected from official android market (Google Play Store) and on various malicious applications. KeywordsDynamic Analysis, Machine learning, Malware,
Design of wideband comb shape substrate integrated waveguide multimode resonator bandpass filter with high selectivity and improved upper stopband performance

In this article, a wideband bandpass filter (BPF) is designed using the comb slotted substrate integrated waveguide (SIW) cavities. The comb‐shaped slots engraved on the SIW cavity are used to constitute a novel multiple‐mode resonator (MMR) that accomplishes a wide passband of operation. Further, a Jerusalem cross defected ground structure (DGS) is introduced to miniaturize it and enhance filter performance in the pass band and stop band. The filter is fabricated on RT/Duroid 5880 having dielectric constant 2.2 and tested to prove the validity of design. The filter achieves 3 dB fractional bandwidth of 48%, return loss above 14 dB and insertion loss of 1.1 dB in the passband. Also, the proposed filter has steep selectivity and wide upper stopband with 25 dB attenuation from 16.7 to 24 GHz.
Análisis in vitro de la elasticidad y la flexión del Peek

El PEEK tiene varias aplicaciones en el ambito de la odontologia debido a su gran biocompatibilidad. No obstante, no se conoce de manera especifica el comportamiento fisico-mecanico de este material. Por ello, el objetivo de este estudio in vitro ha sido evaluar y comparar la resistencia a la flexion y el modulo de elasticidad de distintas muestras fabricadas a partir del PEEK. En este estudio, se fabricaron diez muestras de PEEK con unas dimensiones especificas (longitud x anchura x grosor) obteniendo asi, dos grupos clasificados segun el grosor/espesor de las muestras: un primer grupo con 5 muestras de 24x15x2 mm y otro grupo con 5 muestras de 24x15x15 mm. Seguidamente, todas las muestras fueron sometidas a una prueba de flexion de tres puntos en una maquina de prueba universal Zwick/Roell. Los resultados nos muestran que el primer grupo de probetas de PEEK con un espesor de 2 mm presenta valores mas elevados de modulo de elasticidad y resistencia a la flexion en comparacion con el grupo de probetas de 1,5 mm de grosor.
Estimated GFR and Mortality in Older Men: Are All eGFR Formulae Equal?

Background: Recently, the first estimated glomerular filtration rate (eGFR) formula specifically developed for community-dwelling older adults, the Berlin Initiative Study Equation 2 (BIS2), was reported. To date, however, no study has examined the performance of the BIS2 to predict death in older adults as compared to equations used clinically and in research. Methods: We prospectively followed 2,994 community-dwelling men (age 76.4 ± 5.6) enrolled in the MrOS Sleep Study. We calculated baseline eGFR from serum creatinine and cystatin-C using the BIS2, Chronic Kidney Disease Epidemiology (CKD-EPIcr,cysc), CKD-EPIcysc and CKD-EPIcr equations. Analyses included Cox-proportional hazards regression and net reclassification improvement (NRI) for the outcomes of all-cause and cardiovascular death. Results: Follow-up time was 7.3 ± 1.9 years. By BIS2, 42 and 11% had eGFR <60 and <45, respectively, compared to CKD-EPIcr (23 and 6%), CKD-EPIcysc (36 and 13%) and CKD-EPIcr,cysc (28 and 8%). BIS2 eGFR <45 was associated with twofold higher rate of all-cause mortality when compared to eGFR ≥75 after multivariate adjustment (HR 2.1, 95% CI 1.5-2.8). Results were similar for CKD-EPIcr,cysc <45 (HR 2.1, 95% CI 1.6-2.7) and CKD-EPIcysc <45 (HR 2.1, 95% CI 1.7-2.7) and weaker for CKD-EPIcr <45 (HR 1.5, 95% CI 1.2-2.0). In NRI analyses, when compared to CKD-EPIcr,cysc, both BIS2 and CKD-EPIcr equations more often misclassified participants with respect to mortality. We found similar results for cardiovascular death. Conclusion: The BIS2 did not outperform and the CKD-EPIcr was inferior to the cystatin C-based CKD-EPI equations to predict death in this cohort of older men. Thus, the cystatin C-based CKD-EPI equations are the formulae of choice to predict death in community-dwelling older men.
Exploiting transport-block constraints in LTE improves downlink performance

Efficient resource allocation is necessary to provide the users with the quality of service promised in modern cellular networks, such as LTE. Traditional allocation methods make use of the smallest granularity available, the physical resource block (PRB), to assign resources to each user. The selected resources assigned to a user form a transport block (TB). However, the standard constrains the use of only one modulation and coding rate per TB. This forces the channel quality of the resources to be averaged across the TB, in order to determine the best modulation and coding scheme. In state-of-the-art systems, a non-linear heuristic is used in order to perform this averaging. Unfortunately, when bad PRBs are present next to good ones, this strategy is not optimal. We show that dropping the worst PRBs can improve the performance while remaining standard-compliant. We propose a simple algorithm that can be overlaid to any existing solution and we analyse its effect on multiple state-of-the-art schedulers. A gain is obtained both in throughput (up to 8% increase) and in power consumption (up to 23% reduction).
Optimized GNSS Station Selection to Support Long-Term Monitoring of Ionospheric Anomalies for Aircraft Landing Systems

Differential global navigation satellite systems (GNSS)-based aircraft precision approach and landing systems require the development of ionospheric threat models to insure that users are sufficiently protected against ionospheric anomalies. The long-term ionospheric anomaly monitor (LTIAM) is being used to build ionospheric threat models for ground-based augmentation systems (GBAS) and to continuously monitor ionospheric behavior over the life cycle of GBAS. While LTAIM exhaustively detects all potential anomalies, the use of poor-quality GNSS data degrades the accuracy of ionospheric delay estimates and produces many faulty anomaly candidates, thus adding a great burden to LTIAM processing. To select GNSS reference stations with high-quality data, an optimized set of thresholds for data quality metrics are established. The high-quality station selection method maximizes the elimination of spurious gradients while minimizing unnecessary station removals. When applied to the continuously operating reference stations (CORS) network in the Conterminous U.S. (CONUS), this method discards 90% of faulty candidates while only excluding 14% of the over 1600 CORS stations. The well-distributed subnetwork selection method is also proposed to remove geographically redundant stations in dense regions. The number of CORS stations in CONUS is reduced to 48% of total stations when a desired baseline constraint is 100 km. The results demonstrate that the optimal GNSS station section methods are applicable to a wide range of GNSS station networks that will be used for ionospheric monitoring.
Stars and reionization: the cross-correlation of the 21 cm line and the near-infrared background

With improving telescopes, it may now be possible to observe the Epoch of Reionization in multiple ways. We examine two of these observables - the excess light in the near-infrared background that may be due to high-redshift stars and ionized HII bubbles, and the 21 cm emission from neutral hydrogen. Because these two forms of emission should result from different, mutually exclusive regions, an anticorrelation should exist between them. We discuss the strengths of using cross-correlations between these observations to learn more about high-redshift star formation and reionization history. In particular, we create simulated maps of emission from both the near-infrared background and 21 cm emission. We find that these observations are anticorrelated, with the strongest anticorrelation originating from times when the universe is half ionized. This result is robust and does not depend on the properties of the stars themselves. Rather, it depends on the ionization history. Cross-correlations can provide redshift information, which the near-infrared background cannot provide alone. In addition, cross-correlations can help separate foreground emission from the true high-redshift component, making it possible to say with greater certainty that we are indeed witnessing the Epoch of Reionization.
The Research on the Operating Situation of China’s Real Interest Rate Based on the Three Regimes Markov-switching Model

Since Chinese government implemented interest rate liberalization reform, the level of interest rate has aroused widespread concern from all walks of life. In this paper, we divide the state of China’s real interest rate into three different regimes: low level interest rate regime, medium level interest rate regime and high level interest rate regime. Then we apply three regimes Markov-switching Model to explore the operating situation of China’s real interest rate. The result of empirical research indicates that low level interest rate and high level interest rate are not significant in our country and medium level interest rate is significant and has a long period of duration.
Ring amplifiers for switched-capacitor circuits

To overcome the challenges that CMOS process scaling has imposed on the design of switched-capacitor amplification circuits, designers must consider a growing number of design tradeoffs and employ new circuit techniques in order to achieve required accuracies, often at a cost of added power and complexity. This amplification performance bottleneck has been highlighted in recent years by the disparity in achievable power efficiency between SAR ADCs versus ADC structures that require amplification [1]. In many analog and mixed-signal topics, a comparison like this doesn't even exist, simply because amplification is a necessity. Even SAR ADCs have their limitations, particularly at higher resolutions where matching and noise constraints begin to dominate capacitor sizing and comparator power requirements.
Studies and knowledge fusion in archaeological site maps managed in a 3d GIS, INTERNATIONAL

The protection and conservation attempts of ancient monumental heritage of the city of Hierapolis of Phrygia, during the last few years, are more delineated than in the past as inevitably connected to the environment context, which is characterized by the more extended and significant limestone concretions phenomenon in the world. Studies, excavation works, ancient monuments restorations, carried out through the cooperation between the archaeological Italian Mission (directed by Prof. F. D'Andria, University of Lecce) and the Turkish Cultural Ministry are more and more connected to the safeguard and valorisation of the environmental heritage. In such state, specific and variegate issues are requested to every group; so even the Polytechnic of Turin research unit, involved in the large scale map adjournment and the architectonical metric survey of several city structures, moved objectives and researches in new directions. The actual city map updating, realized mostly by topographical methods for ruins and architectural buildings and by contours extractions from a traditional cartography, needed an updated source of spatial data to obtain the enlargement of the original map to the surroundings areas of archaeological ruins. New fields of spatial data acquisition and data processing and managing have been adopted in order to use and adapt them to the new perspective of archaeological site conservation and management. As the realization of a new photogrammetric flight in the area of Hierapolis is not so simple to achieve for strangers, the best way to obtain a good product at low cost is the use of remote sensing techniques. The objective of the present work is to highlights the following topics: • Orthophoto production from remote sensing data in order to carry out geographical data. • Data integration to supporting thematic studies (streets organizations; digital elevation model and water sliding)
Exploring twisted mass lattice QCD with the Clover term

It has been shown that in the twisted mass formulation of Lattice QCD at maximal twist large cutoff effects are generated when the quark mass becomes of O(a{lambda}{sub QCD}{sup 2}). In general, these effects can be suppressed in two ways: either by choosing the critical quark mass in an ''optimal way'' or by adding the Clover term to the twisted action. We investigate the second option by performing a quenched lattice QCD simulation with twisted Clover fermions and pion masses as low as 280 MeV. We show that the Clover term is indeed efficient in reducing the large cutoff effects. In particular, the so-called bending phenomenon observed in the determination of the pion decay constant is cured in this way. In addition, by using maximally twisted Clover fermions, we provide a nonperturbative determination of the vector current renormalization constant Z{sub V} as well as of the nonperturbatively renormalized light quark masses. Finally, we calculate the connected contribution to the charged-neutral pseudoscalar meson mass splitting, finding that the introduction of the Clover term in the twisted action is also beneficial, in the quenched approximation, in reducing cutoff effects related to the isospin symmetry breaking at finite lattice spacing.
Real-Time Adaptive Apnea and Hypopnea Event Detection Methodology for Portable Sleep Apnea Monitoring Devices

This paper presents a novel real-time adaptive sleep apnea monitoring methodology, suitable for portable devices used in home care applications. The proposed method identifies apnea/hypopnea events with the help of oronasal airflow signal and aimed to meet clinical standards in the assessment mechanism of apnea severity. It uses a strategically combined adaptive two stage classifier model to detect apnea or hypopnea events on the basis of personalized breathing patterns. For the detection of events, optimum set of time, frequency, and nonlinear measures, extracted from overlapping segments of typical 8 s were fed to support vector machine-based classifiers model to identify the possible origin of the segments, i.e., whether from normal or abnormal (apnea/hypopnea) episodes, and then the decision of the classifier model on the time sequenced successive segments have been used to detect an event. The performance of the proposed real-time algorithm is validated on clinical tests online. Average accuracies of hypopnea, apnea, and combined event detection when compared with polysomnography-based respective indices on unseen subjects during online tests were found to be 91.8%, 94.9%, and 96.5%, respectively, which are quite acceptable.
Large-Scale Similarity Search Profiling of ChEMBL Compound Data Sets

A large-scale similarity search investigation has been carried out on 266 well-defined compound activity classes extracted from the ChEMBL database. The analysis was performed using two widely applied two-dimensional (2D) fingerprints that mark opposite ends of the current performance spectrum of these types of fingerprints, i.e., MACCS structural keys and the extended connectivity fingerprint with bond diameter four (ECFP4). For each fingerprint, three nearest neighbor search strategies were applied. On the basis of these search calculations, a similarity search profile of the ChEMBL database was generated. Overall, the fingerprint search campaign was surprisingly successful. In 203 of 266 test cases (∼76%), a compound recovery rate of at least 50% was observed with at least the better performing fingerprint and one search strategy. The similarity search profile also revealed several general trends. For example, fingerprint searching was often characterized by an early enrichment of active compounds in database selection sets. In addition, compound activity classes have been categorized according to different similarity search performance levels, which helps to put the results of benchmark calculations into perspective. Therefore, a compendium of activity classes falling into different search performance categories is provided. On the basis of our large-scale investigation, the performance range of state-of-the-art 2D fingerprinting has been delineated for compound data sets directed against a wide spectrum of pharmaceutical targets.
Effects of low and high dose intraarticular tiludronate on synovial fluid and clinical variables in healthy horses—a preliminary investigation

To determine effects of intraarticularly administered tiludronate on articular cartilage in vivo, eight healthy horses were injected once with tiludronate (low dose tiludronate [LDT] 0.017 mg, n = 4; high dose tiludronate [HDT] 50 mg, n = 4) into one middle carpal joint and with saline into the contralateral joint. Arthrocentesis of both middle carpal joints was performed pre-treatment, and 10 min, 24 h, 48 h, 7 and 14 days after treatment. Synovial nucleated cell counts and total solids, tiludronate, sulfated glycosaminoglycan (sGAG), chondroitin sulfate 846 epitope (CS-846, a measure of aggrecan synthesis), and collagen type II cleavage neoepitope (C2C) concentrations were determined. Histologic analysis of joint tissues and sGAG quantitation in cartilage was performed at 14 days in HDT horses. Data were analyzed by repeated measures non-parametric ANOVA and Wilcoxon signed-rank test. High dose tiludronate administration produced synovial fluid tiludronate concentrations of 2,677,500 ng/mL, exceeding concentrations that were safe for cartilage in vitro, and LDT administration produced synovial fluid concentrations of 1,353 ng/mL, remaining below concentrations considered potentially detrimental to cartilage. With HDT, synovial fluid total solids concentration was higher at 24 h and 7 days and sGAG concentration was higher at 48 h, compared to control joints. Synovial fluid CS-846 concentration was increased over pre-treatment values in HDT control but not in HDT treated joints at 24 and 48 h. All joints (HDT and LDT control and treated) showed a temporary decrease in synovial fluid C2C concentration, compared to pre-treatment values. Histologic features of articular cartilage and synovial membrane did not differ between HDT treated and control joints. High dose tiludronate treatment caused a transient increase in synovial total solids and temporarily increased proteoglycan degradation in cartilage. Although clinical significance of these changes are questionable, as they did not result in articular cartilage damage, further investigation of the safety of intraarticular HDT in a larger number of horses is warranted.