Miracle-dz/UniFAR-FTUs huggingface dataset:
Ruptures of the rotator cuff of the shoulder. New concepts in the diagnosis and operative treatment of chronic ruptures.

Forty-two cases tend to disprove the conception that a diagnosis of a rupture of the rotator cuff should be based on the inability of a patient to abduct the arm well and that the "drop arm" test is a good diagnostic sign. All patients with a chronic rupture had good abduction, but the predominating complaint was pain. Arthrography confirmed the diagnosis. When large defects with retraction were found at operation, anatomical reposition was often impossible. In ten such patients the intra-articular portion of the long head of the biceps was used as a free graft by sectioning the tendon in a book-like fashion. The end results were quite satisfactory in nine patients who obtained a good range of motion with definite decrease in pain.
Performance analysis of uplink systems in the presence of self-interference and co-channel interferences

In practice, each cell in the cellular system will inevitably be interfered by other cells (OCI). In this paper, both self-interference (SI) and co-channel interference (CCI) are considered in MRC systems employing maximal ratio combining under independent and identically distributed Rayleigh fading. Closed-form expressions for probability density function, outage probability of output signal-to-interference plus noise ratio and average symbol error ratio (ASER) of system are presented. Simulation results show that the results are consistent with the numerical simulation.
Análisis de la diversidad genética de Leishmania infantum en Paraguay ediante la técnica del kDNA-PCR-RFLP

Leihmania infantum es el agente causal de la leishmaniasis viceral canina y humana en Paraguay. Una alta proporcion de canes infectados y un aumento en el numero de casos humanos, hacen necesario un control mas eficaz de la enfermedad asi como tambien una evaluacion mas profunda de la epidemiologia de la misma. En la actualidad lo que se busca es la aplicacion de tecnicas moleculares sesnsibles que detecten el ADN del parasito y que permitan caracterizarlo directamente en muestras biologicas. En el presente trabajo se estudio la diversidad genetica de la especie Leishmania infantum, mediante la tecnica de reaccion en cadena de la polimerasa, empleando como blanco molecular los minicirculos del ADNK seguido por cortes con la enzima de restriccion Rsal, con el fin de evaluar la asociacion genotipica de los parasitos encontrados en muestras humanas y caninas. El analisis de las 53 muestras, que incluye tanto a muestras biologicas de humos y caninos ademas de parasitos aislados en cultivo, mostraron un alto grado de polimorfismo genetico con un total de 19 perfiles diferentes separados en 5 clusters. No se observo tendencia de agrupamiento de acuerdo al tipo de muestra, mas bien una tendencia de agrupamiento debido a la region geografica, pero si se observaron perfiles compartidos por parasitos de muestras de humanos y perros, lo que confirma el patron epidemiologico ya conocido. Se observo ademas, una seleccion clonal de los parasitos en cultivo al no presentar los mismos perfiles las formas amastigote, presente en l a muestra biologica y su correspondiente aislado en cultivo, la forma promastigote. El estudio de la variacion genetica en Leishmania no solo podria tener implicancias importantes para el control de la enfermedad, sino que tambien ayudaria a dilucidar la evolucion biologica del agente causal de leishmaniosis visceral.
Clinical, cellular, and bioinformatic analyses reveal involvement of WRAP53 overexpression in carcinogenesis of lung adenocarcinoma

Lung cancer, of which non-small cell lung cancer accounts for 80%, remains a leading cause of cancer-related mortality and morbidity worldwide. Our study revealed that the expression of WD repeat containing antisense to P53 (WRAP53) is higher in lung-adenocarcinoma specimens than in specimens from adjacent non-tumor tissues. The prevalence of WRAP53 overexpression was significantly higher in patients with tumor larger than 3.0 cm than in patients with tumor smaller than 3.0 cm. The depletion of WRAP53 inhibits the proliferation of lung-adenocarcinoma A549 and SPC-A-1 cells via G1/S cell-cycle arrest. Several proteins interacting with WRAP53 were identified through co-immunoprecipitation and liquid chromatography/mass spectrometry. These key proteins indicated previously undiscovered functions of WRAP53. These observations strongly suggested that WRAP53 should be considered a promising target in the prevention or treatment of lung adenocarcinoma.
Aberrant Hypermethylation of Tumor Suppressor Genes in Pancreatic Endocrine Neoplasms

Objective Because histologic features can be unreliable in determining the malignant potential of pancreatic endocrine neoplasms (PENs), we characterized the methylation patterns of PENs to develop a molecular marker system useful for clinical prognosis. Summary Background Data Aberrant promoter methylation of tumor suppressor genes is associated with a loss of gene function that can afford selective growth advantages to neoplastic cells. Gene hypermethylation, coupled with sporadic genetic mutations, defines the heterogeneous biology of human neoplasms. Methods Forty-eight well-differentiated PENs were subjected to methylation-specific polymerase chain reaction to detect aberrant methylation associated with 11 candidate tumor suppressor genes (INK4a/p16, APC, O6-MGMT, hMLH1, p73, E-cadherin, RAR-&bgr;, p14ARF, GST-&pgr;, TIMP3, and RASSF1A). Methylation differences among PENs, subdivided according to tumor size, lymph node status, or liver metastasis, were analyzed, and the association of gene methylation with tumor recurrence and patient survival was evaluated. Results Aberrant hypermethylation of any of the 11 tumor suppressor genes was detected in 87% of the PENs. In decreasing order of frequency, the 5 most commonly methylated genes were: RASSF1A (75%), INK4a/p16 (40%), O6-MGMT (40%), RAR-&bgr; (25%), and hMLH1 (23%). In general, tumors larger than 5 cm and those associated with lymph node or hepatic metastases exhibited a higher frequency of methylation at each promoter site compared with PENs without malignant histologic features. The methylation of specific tumor suppressor genes was an independent predictor of early PEN recurrence and decreased 5-year survival following surgical resection. The accumulation of methylation of multiple tumor suppressor genes was associated with early tumor recurrence and reduced survival among a subpopulation of patients with lymph node-negative PENs. Conclusions Aberrant methylation of multiple tumor suppressor genes is associated with advanced tumor stage and identifies molecularly distinct PENs with identical histologic characteristics. The methylation status of specific tumor suppressor genes is predictive of PEN behavior.
An audit of the implementation of the international consensus group's guidelines on reporting of blood films

Aims This study was carried out as part of the Pathology Benchmarking Review and it audits the implementation of the guidelines for the reporting of blood films published by the International Consensus Group for Haematology Review. Methods Each laboratory completed a questionnaire about the number of blood counts and blood films performed. Information was collected on the criteria that were used for preparing a blood film and whether they had followed the guidelines of the International Consensus Group. Results 74 National Health Service organisations (151 laboratory sites) participated in the study. 24 laboratories had implemented the guideline, 21 with local modification, and 3 without modification. The reasons that prevented the full implementation of the guideline included the inability of laboratory information systems to be modified to include the guideline rules, the laboratory not agreeing with the guideline criteria, and the staff time required to implement the guide to justify the perceived local benefits of implementation. Conclusion This is the first study that has assessed the implementation of the International Consensus Guidelines at a national level. Many laboratories had experienced difficulty in implementing the consensus guideline partly due to the complexity of the guideline. As a result 21 of the 24 laboratories that had attempted to implement the guideline had made local modification to the guideline. Since it was not possible to estimate the impact that local modification of the guideline would have on the percentage of blood films it was not possible to establish a benchmark of practice following implementation of the guideline.
3,6,2′,4′,5′-Pentahydroxyflavone, an Orally Bioavailable Multiple Protein Kinase Inhibitor, Overcomes Gefitinib Resistance in Non-small Cell Lung Cancer*

Background: Gefitinib treatment is limited by primary or acquired TKI resistance in NSCLC. Results: 36245-PHF was identified and investigated in NSCLC, and its underlying mechanism of action was evaluated. Conclusion: 36245-PHF inhibits proliferation and tumor growth of gefitinib-sensitive and -resistant NSCLC cells by targeting PI3K and Aurora A and B. Significance:36245-PHF was identified as a novel therapeutic agent to overcome gefitinib-resistant NSCLC. Non-small cell lung cancer (NSCLC) is the most lethal cancer, causing more than 150,000 deaths in the United States in 2013. The receptor tyrosine kinase inhibitors such as gefitinib are not perfect clinical therapeutic agents for NSCLC treatment due to primary or acquired tyrosine kinase inhibitor resistance. Herein, 3,6,2′,4′,5′-pentahydroxyflavone (36245-PHF) was identified as a multiple kinase inhibitor for NSCLC treatment based on the computational screening of a natural products database. 36245-PHF was shown to inhibit PI3K and Aurora A and B kinases and overcome gefitinib-resistant NSCLC growth. Our data clearly showed that 36245-PHF markedly inhibited anchorage-independent growth of gefitinib-resistant NSCLC cell lines and exerted a substantial chemotherapeutic effect following oral administration in a gefitinib-resistant NSCLC xenograft model. The evidence from three different subsequent methodological approaches, in vitro, ex vivo, and in vivo, all confirmed that 36245-PHF as a multiple protein kinase inhibitor. Overall, we identified 36245-PHF as a multiple protein kinase inhibitor and as a novel therapeutic agent to overcome gefitinib-resistant NSCLC growth, which could provide a new option for clinical NSCLC oral treatment.
Statistical Applications in Genetics and Molecular Biology MA-SNP — A New Genotype Calling Method for Oligonucleotide SNP Arrays Modeling the Batch Effect with a Normal Mixture Model

Genome-wide association studies hold great promise in identifying disease-susceptibility variants and understanding the genetic etiology of complex diseases. Microarray technology enables the genotyping of millions of single nucleotide polymorphisms. Many factors in microarray studies, such as probe selection, sample quality, and experimental process and batch, have substantial effect on the genotype calling accuracy, which is crucial for downstream analyses. Failure to account for the variability of these sources may lead to inaccurate genotype calls and false positive and false negative findings. In this study, we develop a SNP-specific genotype calling algorithm based on the probe intensity composite representation (PICR) model, while using a normal mixture model to account for the variability of batch effect on the genotype calls. We demonstrate our method with SNP array data in a few studies, including the HapMap project, the coronary heart disease and the UK Blood Service Control studies by the Wellcome Trust Case-Control Consortium, and a methylation profiling study. Our single array based approach outperforms PICR and is comparable to the best multi-array genotype calling methods.
WS2 Nanowires as a High-Performance Anode for Sodium-Ion Batteries.

We report the synthesis and anode application for sodium-ion batteries (SIBs) of WS2 nanowires (WS2 NWs). WS2 NWs with very thin diameter of ≈25 nm and expanded interlayer spacing of 0.83 nm were prepared by using a facile solvothermal method followed by a heat treatment. The as-prepared WS2 NWs were evaluated as anode materials of SIBs in two potential windows of 0.01-2.5 V and 0.5-3 V. WS2 NWs displayed a remarkable capacity (605.3 mA h g(-1) at 100 mA g(-1) ) but with irreversible conversion reaction in the potential window of 0.01-2.5 V. In comparison, WS2 NWs showed a reversible intercalation mechanism in the potential window of 0.5-3 V, in which the nanowire-framework is well maintained. In the latter case, the interlayers of WS2 are gradually expanded and exfoliated during repeated charge-discharge cycling. This not only provides more active sites and open channels for the intercalation of Na(+) but also facilitates the electronic and ionic diffusion. Therefore, WS2 NWs exhibited an ultra-long cycle life with high capacity and rate capability in the potential window of 0.5-3 V. This study shows that WS2 NWs are promising as the anode materials of room-temperature SIBs.
Synergistic action of two different types of endo-cellulase components from Irpex lacteus (Polyporus tulipiferae) in the hydrolysis of some insoluble celluloses.

The substrate specificities of three endo-cellulase [EC 3.2. 1.4] components, F-1, F-2, and S-1, obtained from the culture filtrate of Irpex lacteus (Polyporus tulipiferae), were investigated in detail. It was confirmed that the former is of a more random type, belonging to the carboxymethyl-cellulase (CMCcase) group, and the latter two are of a less random type, beloning to the Avicelase group. It was found that a mixture of CMCase and Avicelase shows a remarkable synergistic action in the degradation of cotton and Avicel and that CMCase lowers the degree of polymerization of both cotton and CM-cellulose faster than Avicelases, relative to the production of reducing sugar. Thus, it was assumed that cotton and similar cellulosic substrates were degraded mainly by the synergistic action of these cellulase components produced by this celluloytic fungus.
Prediction of Earnings Per Share for industry

Prediction of Earnings Per Share (EPS) is the fundamental problem in finance industry. Various Data Mining technologies have been widely used in computational finance. This research work aims to predict the future EPS with previous values through the use of data mining technologies, thus to provide decision makers a reference or evidence for their economic strategies and business activity. We created three models LR, RBF and MLP for the regression problem. Our experiments with these models were carried out on the real datasets provided by a software company. The performance assessment was based on Correlation Coefficient and Root Mean Squared Error. These algorithms were validated with the data of six different companies. Some differences between the models have been observed. In most cases, Linear Regression and Multilayer Perceptron are effectively capable of predicting the future EPS. But for the high nonlinear data, MLP gives better performance.
Adult Acquired Flatfoot Deformity at the Talonavicular Joint: Reconstruction of the Spring Ligament in an in Vitro Model

The mobile unilateral flatfoot deformity of chronic posterior tibial tendon insufficiency has been difficult to correct by soft tissue procedures. The procedures can decrease pain, but they do not always correct the longitudinal arch or relieve all the symptoms. Using 10 fresh frozen cadaveric specimens and a rig for stimulation of weightbearing, the deformity associated with chronic posterior tibial tendon insufficiency was produced by multiple ligamentous release and documented by AP and lateral radiographs. Reconstruction of the spring ligament using a ligament bone autograft from the superficial deltoid ligament was then performed and tested under load. The mean correction was within 2.5° of normal (over or undercorrection) on both the AP and lateral radiographs with the specimens under load. Clinical Relevance. In posterior tibial tendon insufficiency, it may be possible to address the ligament as well as tendon insufficiency to gain a corrected arch. The success of such a procedure will depend upon adequate tendon and ligament reconstruction in a fully mobile deformity. Questions remain as to the adequacy of this ligament graft, and a stronger free ligament graft, as well as correction of any bony malalignment, may be required.
Reinforcement Learning: A Novel Method for Optimal Control of Propofol-Induced Hypnosis

Reinforcement learning (RL) is an intelligent systems technique with a history of success in difficult robotic control problems. Similar machine learning techniques, such as artificial neural networks and fuzzy logic, have been successfully applied to clinical control problems. Although RL presents a mathematically robust method of achieving optimal control in systems challenged with noise, nonlinearity, time delay, and uncertainty, no application of RL in clinical anesthesia has been reported.
Human Intelectin Is a Novel Soluble Lectin That Recognizes Galactofuranose in Carbohydrate Chains of Bacterial Cell Wall*

Galactofuranosyl residues are present in various microorganisms but not in mammals. In this study, we identified a human lectin binding to galactofuranosyl residues and named this protein human intelectin (hIntL). The mature hIntL was a secretory glycoprotein consisting of 295 amino acids and N-linked oligosaccharides, and its basic structural unit was a 120-kDa homotrimer in which 40-kDa polypeptides were bridged by disulfide bonds. The hIntL gene was split into 8 exons on chromosome 1q21.3, and hIntL mRNA was expressed in the heart, small intestine, colon, and thymus. hIntL showed high levels of homology with mouse intelectin,Xenopus laevis cortical granule lectin/oocyte lectin, lamprey serum lectin, and ascidian galactose-specific lectin. These homologues commonly contained no carbohydrate recognition domain, which is a characteristic of C-type lectins, although some of them have been reported as Ca2+-dependent lectins. Recombinant hIntL revealed affinities to d-pentoses and ad-galactofuranosyl residue in the presence of Ca2+, and recognized the bacterial arabinogalactan ofNocardia containing d-galactofuranosyl residues. These results suggested that hIntL is a new type lectin recognizing galactofuranose, and that hIntL plays a role in the recognition of bacteria-specific components in the host.
The integration of a woman's perspective in hysterectomy decisions

It has been argued, based on a limited amount of research data, that patient participation in decision-making regarding surgery, results not only in more patient satisfaction, but also in better surgery outcomes. Especially in a procedure such as hysterectomy, with far-reaching consequences and many potential side effects, it may therefore be relevant to promote an active role of the patient in the decision-making process. The article identifies contextual elements that might promote this active role. Subsequently a model of participative, informed decision-making is outlined. The model entails that clinical guidelines should be adjusted to the particular situation at hand and that patients should be fully informed about hysterectomy-involved risks. An overview of the various guidelines and risks is provided. Proper counseling in the decision-making phase as well as in the post-hysterectomy period is advocated. Throughout the article attention is paid to documented and undocumented ethnic differences regarding various aspects of hysterectomy, particularly differences in patient participation around the world.
Massively parallel sequencing of ataxia genes after array‐based enrichment

Massively parallel sequencing has tremendous diagnostic potential but requires enriched templates for sequencing. Here we report the validation of an array‐based sequence capture method in genetically heterogeneous disorders. The model disorder selected was AR ataxia, using five subjects with known mutations and two unaffected controls. The genomic sequences of seven disease genes, together with two control loci were targeted on a 2‐Mb sequence‐capture array. After enrichment, the patients' DNA samples were analyzed using one‐quarter Roche GS FLX Titanium sequencing run, resulting in an average of 65 Mb of sequence data per patient. This was sufficient for an average 25‐fold coverage/base in all targeted regions. Enrichment showed high specificity; on average, 80% of uniquely mapped reads were on target. Importantly, this approach enabled automated detection of deletions and hetero‐ and homozygous point mutations for 6/7 mutant alleles, and greater than 99% accuracy for known SNP variants. Our results also clearly show reduced coverage for sequences in repeat‐rich regions, which significantly impacts the reliable detection of genomic variants. Based on these findings we recommend a minimal coverage of 15‐fold for diagnostic implementation of this technology. We conclude that massive parallel sequencing of enriched samples enables personalized diagnosis of heterogeneous genetic disorders and qualifies for rapid diagnostic implementation. Hum Mutat 31:1–8, 2010. © 2010 Wiley‐Liss, Inc.
Sound Production in the Clownfish Amphiprion clarkii

Although clownfish sounds were recorded as early as 1930, the mechanism of sound production has remained obscure. Yet, clownfish are prolific "singers" that produce a wide variety of sounds, described as "chirps" and "pops" in both reproductive and agonistic behavioral contexts. Here, we describe the sonic mechanism of the clownfish Amphiprion clarkii.
Double-Strand Break Yield Following 125I Decay Effects of DNA Conformation

The decay of iodine-125 ( 125 I) is accompanied by the emission of low-energy electrons that dissipate most of their energy in approximately 10 nm from the decay site. In mammalian cells the . OH generated by these electrons are also confined to a small volume. Iodine-125 is thus an excellent probe for assessing the radiobiologic effects produced by . OH in close proximity to the site of a decaying atom. We have compared in pUC19 plasmids (naked DNA) and in Chinese hamster V79 lung fibroblasts (chromatin) the modulation by the . OH scavenger dimethyl sulfoxide (DMSO) of 125 I-induced DNA double-strand breaks (DSB). The data indicate that DMSO cannot protect plasmid DNA against DSB damage from 125 I decaying within a few angstroms from DNA. However, DMSO attenuated DSB production in V79 cells following the decay of DNA-incorporated 125 I, thus suggesting that chromatin structure fosters some DSB formation by indirect mechanism(s). DSB production depends on the environment and/or conformation of DNA. Consequently, current biophysical modeling of DNA damage that is based on naked and non-compacted DNA is inadequate for explaining radiobiologic effects at the cellular level.