Miracle-dz/UniFAR-FTUs huggingface dataset:
TISSUE DISTRIBUTION AND HEPATIC AND RENAL ONTOGENY OF THE MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN (MRP) FAMILY IN MICE

Analysis of the mouse genome has revealed eight multidrug resistance-associated (Mrp) transporters, with mouse homologs for all human MRPs except MRP8. Whereas MRP expression in tissues of humans and rats has been examined, no characterization exists for mice. Furthermore, the ontogeny of mouse Mrps is unknown, and such knowledge may be helpful in understanding age-related pharmacokinetics. Therefore, the purpose of this study was to quantitatively determine 1) expression of the Mrp family in 12 different tissues, 2) gender variations in Mrp expression in liver and kidney, and 3) whether Mrp expression is altered during development. Highest expression of the Mrp family members is as follows: Mrp1 in testes, ovary, and placenta; Mrp2 in intestine, followed by liver and kidney; Mrp3 in large intestine; Mrp4 in kidney; Mrp5 in brain, followed by lung and stomach; Mrp6 in liver; Mrp7 in testes, intestine, and kidney; and Mrp9 solely in testes. Gender differences in Mrp expression were observed: Mrp1, 3, and 4 in kidney, as well as Mrp1 and 4 in liver were female-predominant. Ontogeny of the four Mrps expressed in liver was as follows: Mrp2 and Mrp4 were expressed at adult levels at birth; Mrp3 reached adult levels at day 30, and Mrp6 was not expressed until day 10. In kidney, Mrp1 and Mrp5 were expressed at adult levels at birth, whereas Mrp2, 3, 4, and 6 generally increased over time. In conclusion, marked differences in expression of the individual Mrp family members exist in various tissues, with age, and with gender.
Cardiovascular, hormonal, and metabolic responses to severe prolonged hemorrhage in adult sheep.

Over a 54-hour period, blood was removed from 8 adult sheep (body weight, 38.1 +/- 0.5 kg, mean +/- SEM) in 9 episodes, 5 on day 1, 3 on day 2, and 1 on day 3. Cumulative blood loss was 1,630 +/- 63, 2,380 +/- 71, and 2,693 +/- 69 ml on days 1, 2, and 3, respectively. Blood samples (20 ml) were collected from 5 control ewes (33.8 +/- 2.8 kg) at equivalent times. Over the first day, mean arterial blood pressure decreased in the hemorrhaged sheep from 101 +/- 2 mm of Hg to 76 +/- 5 mm of Hg, but returned to control values by the beginning of the second day and, thereafter, was not different from control values. Heart rate was increased after the first hemorrhage episode and remained high throughout the entire protocol. Over the entire period, there were statistically significant decreases in hematocrit, plasma osmolality, sodium, total calcium (P < 0.001), potassium, and chloride values (P < 0.05). There was no change in plasma phosphate, bicarbonate, creatinine, or magnesium concentrations and an increase in plasma urea nitrogen (P < 0.001) concentrations. Plasma arginine vasopressin concentration was increased significantly (P < 0.001) over the entire period. Plasma ACTH concentration was significantly (P < 0.05) increased over time, but only some values on day 1 were significantly outside the normal range of the control group data. Because of wide variation between sheep, the group data for aldosterone were not significantly different from control values. (ABSTRACT TRUNCATED AT 250 WORDS)
Polymer Thick Film Technology for Realization of Fine Line Structures on Different Substrates

The polymer-thick-film-technology (PTF) is a potential solution for realizing interconnections on substrates under consideration of some special aspects. Such aspects are the production costs and environmental requirements. This paper will analyze the printable resolution of up-to-date PTF pastes, the conductivity and the reliability of the realized structures. Therefore the technological properties of the pastes applied on flexible and rigid substrates are analyzed. It is shown that it is possible to realize structures with lines widths <100 mum and vias with diameters <200 mum using PTF technology. Furthermore the development of new materials allows the screen printing of high conductivity structures.
Relationship between spectral transmittance and slit lamp color of human lenses.

PURPOSE To study the relationship between subjective lens color as observed with slit lamp biomicroscopy and spectral transmittance of the lens. To propose a model for this relationship to derive quantitative information on lens pigmentation from slit lamp observation. METHODS Twenty-nine normal lenses, from donors aged 14 to 86 years, were used. The fraction of light transmitted from a narrow beam was measured as function of wavelength. The spectra were fitted with the one-parameter TL model of Pokorny et al. The relationship between this parameter and the color grading from Chylack et al. (lens opacity classification system III nuclear color score) was established. RESULTS After slight adaptation of the TL model, the shapes of the transmittance spectra corresponded closely to the TL model (average residual error 0.05 log units). Log transmittance and lens opacity classification system nuclear color score were closely related (r = 0.90, 0.77, and 0.55 for 400, 500, and 602 nm, respectively). CONCLUSIONS A mathematical relationship between TL parameter and lens opacity classification system nuclear color score could be established to predict lens transmittance from lens opacity classification system nuclear color score. This relationship was successful in predicting the correction for lens absorption needed in blue-on-yellow perimetry.
Periodontopathic bacteria in children with Down syndrome.

BACKGROUND It is widely known that individuals with Down syndrome (DS) often develop severe early-onset periodontal diseases. In this study, we examined the prevalence of periodontopathic bacteria in DS children to determine if specific pathogens are acquired in their childhood. METHODS The subjects were 60 DS children (2 to 13 years old, 5 in each age bracket) and 60 age-matched controls. Ten pathogens, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Bacteroides forsythus, Treponema denticola, Prevotella intermedia, P nigrescens, Capnocytophaga ochracea, C. sputigena, Campyrobacter rectus, and Eikenella corrodens were surveyed in subgingival plaque samples using a polymerase chain reaction. Periodontal status was evaluated by probing depth, bleeding on probing, and gingival index. RESULTS No significant difference in periodontal status was observed between the DS and control groups, however, all of the pathogens were detected with greater frequency in the DS children. B. forsythus, T. denticola, P. nigrescens, and C. rectus were significantly prevalent throughout all age brackets of the DS children (P <0.01 or 0.05). The occurrence of P. gingivalis was also significant in the DS subjects over 5 years old. A cluster analysis of the microbial profiles of the DS subjects showed that gingivitis severity was associated with increased varieties of the harboring pathogens and the distribution of P. gingivalis. CONCLUSIONS These results suggest that various periodontopathogens can colonize in the very early childhood of DS patients and maturation of subgingival components, including P. gingivalis, plays an important role in the initiation of gingival inflammation.
Psychotic symptoms and population risk for suicide attempt: a prospective cohort study.

IMPORTANCE Up to 1 million persons die by suicide annually. However, a lack of risk markers makes suicide risk assessment one of the most difficult areas of clinical practice. OBJECTIVE To assess psychotic symptoms (attenuated or frank) as a clinical marker of risk for suicide attempt. DESIGN, SETTING, AND PARTICIPANTS Prospective cohort study of 1112 school-based adolescents (aged 13-16 years), assessed at baseline and at 3 and 12 months for self-reported psychopathology, psychotic symptoms, and suicide attempts. MAIN OUTCOMES AND MEASURES Suicide attempts at the 3- and 12-month follow-up and acute suicide attempts (defined as those occurring in the 2 weeks before an assessment). RESULTS Of the total sample, 7% reported psychotic symptoms at baseline. Of that subsample, 7% reported a suicide attempt by the 3-month follow-up compared with 1% of the rest of the sample (odds ratio [OR], 10.01; 95% CI, 2.24-45.49), and 20% reported a suicide attempt by the 12-month follow-up compared with 2.5% of the rest of the sample (OR, 11.27; 95% CI, 4.44-28.62). Among adolescents with baseline psychopathology who reported psychotic symptoms, 14% reported a suicide attempt by 3 months (OR, 17.91; 95% CI, 3.61-88.82) and 34% reported a suicide attempt by 12 months (OR, 32.67; 95% CI, 10.42-102.41). Adolescents with psychopathology who reported psychotic symptoms had a nearly 70-fold increased odds of acute suicide attempts (OR, 67.50; 95% CI, 11.41-399.21). Differences were not explained by nonpsychotic psychiatric symptom burden, multimorbidity, or substance use. In a causative model, the population-attributable fraction of suicide attempts would be 56% to 75% for psychotic symptoms. CONCLUSIONS AND RELEVANCE Adolescents with psychopathology who report psychotic symptoms are at clinical high risk for suicide attempts. More careful clinical assessment of psychotic symptoms (attenuated or frank) in mental health services and better understanding of their pathological significance are urgently needed.
Global Clusters of Innovation: Lessons from Silicon Valley

Can innovation and entrepreneurship stimulate economic growth in diverse communities, or is it only effective in a few unique places like Silicon Valley? This article identifies the salient components, behaviors, and linkages that characterize Silicon Valley and explores how these characteristics apply in a diverse selection of economic communities in Europe, Asia, and Latin America. It focuses on the role institutions—such as governments, universities, major corporations, and NGOs—play in shaping such communities. It provides insights for government policy makers on how to enhance their region's innovation potential, and offers strategies for entrepreneurs and venture investors as to how to leverage the benefits of clusters of innovation, wherever one is located.
Undifferentiated carcinoma of the endometrium. A histopathologic and clinical study of 31 cases

A histopathologic review of 1985 cases of endometrial carcinoma yielded 31 undifferentiated carcinomas (1.6%). Forty‐eight percent were large cell type and 52%, intermediate/small cell type. Twenty‐one tumors were examined immunohistochemically. All stained for keratin. Eleven tumors reacted with vimentin antibodies, two with carcinoembryonic antigen antibodies, and ten with neuron‐specific enolase (NSE) antibodies (four of which stained for bombesin, two for beta‐endorphin, one for prealbumin, five for Leu7, and four for synaptophysin). The mean age at diagnosis was 63.9 years (range, 45 to 86). The crude 5‐year and 10‐year survival was 58% and 48%, respectively. Seventy‐nine percent of the patients in surgicopathologic Stage I and 33% in Stage II survived 5 years. The intermediate/small cell types had a somewhat better prognosis than the large cell type, but the difference was not statistically significant. The presence or absence of NSE and vimentin immunoreactivity had no influence on survival. All patients with tumors infiltrating less than one half of the myometrium survived 5 years in contrast with 46% of the patients with deep infiltrating tumors. Fifty‐four percent of the patients with demonstrable vessel invasion survived 5 years in contrast with 89% not so affected.
Mastering the Integration Chaos Following Frequent M&As: IS Integration with SOA Technology

The use of service oriented architectures (SOA) is rapidly penetrating contemporary business life based on the promise of scalable and flexible information infrastructures. However the technique is still in it infancy and many questions regarding the consequences of a shift towards this kind of IT architecture still remains unanswered. We approach the relation between SOA and another actual topic, namely corporate mergers and acquisitions (M&A). Based on a literature review and an empirical study of five industrial companies we conclude seven relationships between properties of SOA and M&A, which should be valuable both for business professionals considering the SOA alternative and to advance the academic domain that is concerned with integration and M&A
Public Participation in the Selection of Sustainable Development Indicators in Limerick and Freshford, Ireland: Implications for Policy on Advancing Sustainability

The programme comprises three key measures: Sustainable Development, Cleaner Production and Environmental Technologies, and A Healthy Environment; together with two supporting measures: EPA Environmental Research Centre (ERC) and Capacity & Capability Building. The seven principal thematic areas for the programme are Climate Change; Waste, Resource Management and Chemicals; Water Quality and the Aquatic Environment; Air Quality, Atmospheric Deposition and Noise; Impacts on Biodiversity; Soils and Land-use; and Socio-economic Considerations. In addition, other emerging issues will be addressed as the need arises.
Prevention of deaths from harmful drinking in the United States: the potential effects of tax increases and advertising bans on young drinkers.

OBJECTIVE Harmful alcohol consumption is a leading cause of death in the United States. The majority of people who die from alcohol use begin drinking in their youth. In this study, we estimate the impact of interventions to reduce the prevalence of drinking among youth on subsequent drinking patterns and alcohol-attributable mortality. METHOD We first estimated the effect of public health interventions to decrease harmful drinking among youth from literature reviews and used life table methods to estimate alcohol-attributable years of life lost by age 80 years among the cohort of approximately 4 million U.S. residents aged 20 in the year 2000. Then, from national survey data on transitions in drinking habits by age, we modeled the impact of interventions on alcohol-attributable mortality. RESULTS A tax increase and an advertising ban were the most effective interventions identified. In the absence of intervention, there would be 55,259 alcohol-attributable deaths over the lifetime of the cohort. A tax-based 17% increase in the price of alcohol of dollar 1 per six pack of beer could reduce deaths from harmful drinking by 1,490, equivalent to 31,130 discounted years of potential life saved or 3.3% of current alcohol-attributable mortality. A complete ban on alcohol advertising would reduce deaths from harmful drinking by 7,609 and result in a 16.4% decrease in alcohol-related life-years lost. A partial advertising ban would result in a 4% reduction in alcohol-related life-years lost. CONCLUSIONS Interventions to prevent harmful drinking by youth can result in reductions in adult mortality. Among interventions shown to be successful in reducing youthful drinking prevalence, advertising bans appear to have the greatest potential for premature mortality reduction.
Isolated Modular Multilevel DC–DC Converter With DC Fault Current Control Capability Based on Current-Fed Dual Active Bridge for MVDC Application

In this paper, a current-fed modular multilevel dual-active-bridge (CF-MDAB) dc–dc converter is proposed for medium-voltage dc (MVDC) application. The proposed converter inherits favorite characteristics of DAB circuits including soft switching and small passive components. Thereby, high efficiency and high power density can be achieved. Moreover, with direct input and output dc current control, the CF-MDAB is suitable for a breakerless MVDC system since it can realize dc fault ride-though operation. In addition, the dv/dt in the converter is mitigated with the quasi-three-level modulation. In this paper, the proposed converter is applied to integrate the battery energy storage to a MVDC grid as an example to illustrate its operation principles and fault current control capability. The operation principles are presented for both normal and dc fault conditions; the dynamic models are also derived not only under normal operation mode but under dc fault operation mode as well. The control systems under different operation modes are designed, respectively, based on the developed mathematical models. A downscaled 40-kHz 3-kW CF-MDAB prototype was built in the laboratory. The experimental results under both normal condition and dc fault condition verified the analysis as well as the control performance of the proposed converter.
Multi-point response correction for cost-efficient antenna and microwave design optimization

Microwave design optimization using variable-fidelity electromagnetic (EM) simulations and multi-point response correction is presented. In our approach, optimization of the computationally expensive high-fidelity EM model is replaced by iterative design improvement of the low-fidelity model, which is obtained from coarse-discretization EM simulation data. The low-fidelity model is enhanced through a multi-point response correction that utilizes all high-fidelity model data accumulated during the optimization run. The model parameters are obtained analytically by solving an appropriate linear regression problem. Optional frequency scaling is also used to further improve the model alignment. The multi-point correction ensures better generalization than the traditional single-point correction and results in reduction of the computational cost of the design process. Our considerations are illustrated using two examples: a dielectric resonator antenna and a microstrip filter.
Investigation of the vivaldi array for Square Kilometer Array (SKA) application using the parallelized FDTD code GEMS on the LOFAR blue gene/L supercomputer

This paper presents the numerical simulation results of a Vivaldi 9x8x2 array. This type of arrays is being designed by ASTRON for the square kilometer array (SKA). The simulation has been carried out by using general electromagnetic solver (GEMS)-a parallelized finite difference time domain (FDTD) solver (W. Yu et al., 2005)-running on the LOFAR BlueGene/L supercomputer located at the University of Groningen. This supercomputer is one of the world's fastest as well as largest-it has up to 6000 nodes- and the high parallelization efficiency of GEMS (-90%) makes the combination a very powerful tool for solving large complex problems in a time-efficient manner. The array parameters studied include the input impedance of the active element, coupling between the active element and those surrounding it, and the far field pattern of the same element, when the rest of the array elements are terminated with its characteristic impedance. Numerical results generated from the simulations using GEMS are presented in the paper and are compared with the measurement results.
A Novel Ca2+-Independent Signaling Pathway to Extracellular Signal-Regulated Protein Kinase by Coactivation of NMDA Receptors and Metabotropic Glutamate Receptor 5 in Neurons

The specification and organization of glutamatergic synaptic transmission require the coordinated interaction among glutamate receptors and their synaptic adaptor proteins closely assembled in the postsynaptic density (PSD). Here we investigated the interaction between NMDA receptors and metabotropic glutamate receptor 5 (mGluR5) in the integral regulation of extracellular signal-regulated protein kinase (ERK) and gene expression in cultured rat striatal neurons. We found that coapplication of NMDA and the mGluR5 agonist (S)-3,5-dihydroxyphenylglycine synergistically increased ERK phosphorylation. Interestingly, the synergistic increase in ERK phosphorylation was dependent on the cross talk between NMDA receptor-associated synaptic adaptor protein PSD-95 and the mGluR5-linked adaptor protein Homer1b/c but not on the conventional Ca2+ signaling derived from NMDA receptors (Ca2+ influx) and mGluR5 (intracellular Ca2+ release). This was demonstrated by the findings that the synergistic phosphorylation of ERK induced by coactivation of NMDA receptors and mGluR5 was blocked by either a Tat peptide that disrupts NMDA receptor/PSD-95 binding or small interfering RNAs that selectively reduce cellular levels of Homer1b/c. Furthermore, ERK activated through this PSD-95/Homer1b/c-dependent and Ca2+-independent pathway was able to phosphorylate the two key transcription factors Elk-1 and cAMP response element-binding protein, which further leads to facilitation of c-Fos expression. Together, we have identified a novel Ca2+-independent signaling pathway to ERK by the synergistic interaction of NMDA receptors and mGluR5 via their adaptor proteins in the PSD of neurons, which underlies a synapse-to-nucleus communication important for the transcriptional regulation.
Frequency of cells positive for HIV‐1 p24 antigen assessed by flow cytometry

ObjectiveMarkers of HIV disease progression such as soluble p24 antigen detection and CD4 lymphocyte depletion are most useful in the later stages of HIV disease and are relatively insensitive as therapeutic monitors. Flow cytometric detection of HIV-1 replication in CD4 lymphocytes was evaluated for use as a marker in predicting disease progression earlier in the course of HIV disease. DesignTo determine whether the number of HIV-1-infected CD4 cells, as measured by p24 antigen detection, can be correlated with disease progression, we used flow cytometry to detect intracellular HIV-1 p24 in CD4 lymphocytes from HIV-1-seropositive subjects at all stages of HIV disease. MethodsMononuclear cells from HIV-1-seropositive subjects and uninfected control subjects were permeabilized and stained with anti-HIV-1 p24 monoclonal antibodies. The cells were then stained with a fluorescein isothiocyanate-conjugated goat antimurine immunoglobulin G followed by a phycoerythrin-conjugated monoclonal anti-CD4 antibody. The percentage of p24-positive CD4 lymphocytes was compared with absolute CD4 counts, soluble p24 detection and Walter Reed classification. ResultsCD4 lymphocyte absolute counts and the percentage of CD4 lymphocytes declined as the Walter Reed classification indicated disease progression. The mean percentage of p24 antigen-positive CD4 lymphocytes increased with disease progression. Only 30% of Walter Reed stage 6 subjects were soluble p24 antigen-positive, whereas 68% were cellular p24 antigen-positive. ConclusionThe percentage of p24 antigen-positive CD4 lymphocytes increased as HIV disease progressed. Flow cytometric quantitation of p24 antigen-positive CD4 cells is a useful method of monitoring in vivo HIV replication and disease progression.