Miracle-dz/UniFAR-FTUs huggingface dataset:
Correlative microscopy reveals abnormalities in type 1 diabetes

Microscopische technieken zijn van grote waarde voor onderzoek naar de regulatie van leven en ziekte op het niveau van cellen en moleculen. Ons lab maakt geroutineerd gebruik van een manier om op grote schaal elektronenmicroscopie (EM) data op te nemen, nanotomie voor nano-anatomie. Een database met donor materiaal van type 1 diabetes (T1D) patienten werd aangemaakt om het ziekteverloop op hoge resolutie te kunnen bestuderen. Echter, deze grote hoeveelheid data is alleen in grijswaardes en daardoor moeilijk te analyseren. Met gecorreleerde licht microscopie (LM) en EM (CLEM) worden moleculen specifiek zichtbaar gemaakt met behulp van fluorescentie wat vervolgens in een hoge resolutie context geplaatst met EM. Het doel van dit proefschrift was om nieuwe CLEM probes en targeting technieken te ontwikkelen en te implementeren om de analyse van onder andere de nanotomie data te verbeteren. Na het labelen van reeds ingebed materiaal met quantum dots, gecombineerd met een nieuw ontwikkelde ‘kleurenEM’ toepassing werden in nanotomie datasets van zowel een T1D rat model als in het humane donormateriaal afwijkende cellen gevonden in de eilandjes van Langerhans met zowel exocriene als endocriene karakteristieken. Eerste resultaten van functionele vervolgproeven suggereren dat schade aan de exocriene pancreas mogelijk een pro-inflammatoire reactie door insuline producerende betacellen kan veroorzaken in een vroeg stadium van T1D. Samenvattend geeft dit proefschrift ten eerste een overzicht van recente CLEM ontwikkelingen inclusief een praktische handleiding. Daarnaast werd efficiente labeling op EM materiaal geoptimaliseerd in combinatie met verbeterde detectie methodes, wat vervolgens tot nieuwe inzichten in de T1D pathogenese heeft geleid.
Does perceived risk really matter in travel behaviour?

In spite of its importance in understanding tourist behaviour, the scientific knowledge about risk perceptions about a destination has begun to expand especially after 1960s. Especially, far more research is yet needed for exploring the role of risk on potential tourists’ travel motivations, destination image perceptions and visit intentions. This study, therefore, attempts to clarify the moderated impact of risk perception in the relationship among travel motivations, destination image and travel intentions. With this purpose, two survey studies were conducted on German tourists visiting Turkey, whereas the participants evaluated their perceptions about Spain or Greece. The results in both cases showed a high direct influence of travel motivations on destination image perception and a significant direct impact of destination image perception on travel intention. The findings also revealed that risk perception has a negative moderated effect on travel motivations, destination image and travel intention relationships.
Decision Support Systems in clinical engineering

Decision Support Systems (DSS) are very important in clinical engineering (CE). The Analytic Hierarchy Process (AHP) is one of such systems that has been extensively and successfully used in many areas, including CE. In this paper, we provide an overview of different DSS and explain AHP in details. At the end, a case study using AHP for taking medical equipment scrapping/ retirement decision is presented.
Assessing Competition with the Panzar-Rosse Model: The Role of Scale, Costs, and Equilibrium

Abstract The Panzar-Rosse test has been widely applied to assess competitive conduct, often in specifications controlling for firm scale or using a price equation. We show that neither a price equation nor a scaled revenue function yields a valid measure for competitive conduct. Moreover, even an unscaled revenue function generally requires additional information about costs and market equilibrium to infer the degree of competition. Our theoretical findings are confirmed by an empirical analysis of competition in banking, using a sample containing more than 100,000 bank-year observations on more than 17,000 banks in 63 countries during the years 1994 to 2004.
The Myelin‐Deficient Rat Has a Single Base Substitution in the Third Exon of the Myelin Proteolipid Protein Gene

Abstract: Several genetic disorders that occur in animals and in humans result in an inability to synthesize normal myelin. Some of these disorders are inherited in an X‐linked manner. The localization of the myelin proteolipid protein (PLP) gene to the X chromosome has directed the study of X‐linked myelination disorders toward PLP. The myelin‐deficient rat is one such X‐linked dysmyelinating mutant. From a cDNA library constructed from myelin‐deficient rat brain mRNA, we have isolated and sequenced cDNAs corresponding to PLP and its alternatively spliced isoform, DM‐20. An A to C transition was detected in these cDNAs, which results in a threonine to proline change at amino acid 74 in both PLP and DM‐20. No other substitutions were seen in the cDNA sequences. Polymerase chain reaction amplification and sequencing of the corresponding genomic regions were used to confirm the single base change. This substitution occurs in a highly hydrophobic portion of the protein that is thought to be an α‐helical transmembrane segment. The presence of a helix‐breaking amino acid such as proline in this segment is likely to influence the ability of the protein to interact with the membrane.
One-dimensional tunable ferroelectric photonic crystals based on Ba0.7Sr0.3TiO3/MgO multilayer thin films

Tunable photonic crystals (PCs) have attracted much attention in the past decade because of their various applications, such as ultrafast optical filters and optical waveguides with add–drop functionalities. One way of achieving tunability is to make use of ferroelectric materials since the refractive index of ferroelectric materials can be electrically tuned through the electro-optic effect. In this paper, we present our work on developing a tunable one-dimensional (1D) PC based on a Ba0.7Sr0. 3TiO3/MgO multilayer structure. The photonic band structures and band gap maps of the PC were calculated by using the plane-wave expansion (PWE) method. It is found that the gap center linearly shifts with the change in the refractive index of Ba0.7Sr0. 3TiO3. A ferroelectric 1D PC consisting of a Ba0.7Sr0. 3TiO3/MgO multilayer thin film was epitaxially deposited on a MgO (001) single-crystal substrate by pulsed laser deposition. A photonic band gap in the visible region is observed in the transmission spectrum of the.
Illness Representations, Treatment Beliefs, Medication Adherence, and 30-Day Hospital Readmission in Adults With Chronic Heart Failure: A Prospective Correlational Study

Background:An estimated 5.1 million Americans have chronic heart failure, yet despite advances in its treatment, there has been no improvement in hospital readmissions among aging adult patients with chronic heart failure. Objectives:The purpose of this study is to investigate the relationships among illness representations, treatment beliefs, medication adherence, and 30-day hospital readmission for heart failure exacerbation in aging adults with chronic heart failure. Method:Using a prospective, correlational design, 96 older adults with a primary or secondary diagnosis of heart failure discharged to home from a hospital in the Southeastern United States participated in telephone surveys and follow-up telephone calls. Data analysis included correlation and logistic regression analyses. Results:Participants were highly adherent to their medications. Individuals who did not believe their treatment was effective in controlling their HF were readmitted within 30 days of hospital discharge for HF exacerbation; that is, treatment control was inversely related to 30-day hospital readmission. In post hoc analyses, personal control was inversely related to dichotomized medication adherence and necessity-concern differential was directly related to dichotomized medication adherence. The necessity-concern differential, or the belief that medication necessity outweighed the concerns they had about their medicines, was a significant predictor of medication adherence. Discussion:Nurses can use these study findings to help identify individuals who may be at risk of being nonadherent to their medications and hospital readmission. Recommendations for future research include replication with multiple sites, the addition of objective measures of medication adherence, investigation of both the cognitive and emotional pathways, and qualitative exploration of personal control in the context of medication adherence in HF.
Identification of uidA gene sequences in beta-D-glucuronidase-negative Escherichia coli

A probe specific for the uidA gene of Escherichia coli hybridized with 112 of 116 E. coli isolates examined, including 31 beta-D-glucuronidase-negative and 12 enterohemorrhagic E. coli serotype O157:H7 isolates. Southern hybridizations confirmed the presence of a 900-bp HinfI fragment from the uidA gene in all isolates examined, suggesting that uidA gene sequences are present in most E. coli.
Experience using a novel web-based tutorial and assessment tool for advanced electronics teaching

The number of students enrolled at Auckland University School of Engineering in general and in the Department of Electrical and Electronic Engineering, in particular, has increased significantly. Different methods of learning and assessment that help both the students and the educators need to be explored. At the Department of Electrical and Electronic Engineering, OASIS (online assessment integrated system), a computer-based tool was developed to meet those needs. Its successful usage at the introductory level encouraged the authors to use it in a more advanced course. A survey was conducted to establish the usefulness of OASIS. The results of this survey show that OASIS is useful tool both for the students as well as for the educators. The students find that it assists them in the learning process by encouraging them to solve more problems. Also, the assessment in advanced and design style courses could be improved with inclusion of an OASIS component in the course.
Comment on “The Local Structure of Amorphous Silicon”

Treacy and Borisenko (Reports, 24 February 2012, p. 950) argue from reverse Monte Carlo modeling of electron diffraction and fluctuation electron microscopy data that amorphous silicon is paracrystalline and not described by a continuous random network. However, their models disagree with high-resolution x-ray measurements and other evidence, whereas the agreement with fluctuation electron microscopy is at best qualitative.
La nuit n'est pas noire: le rayonnement de fond extragalactique et l'évolution des galaxies infrarouges

L'Univers, en dehors de notre Galaxie, est baigne de nombreux rayonnements, le principal etant observable dans le domaine des ondes radio centimetriques et millimetriques: le fond cosmologique (ou CMB pour {\it Cosmic Microwave Background}), corps noir de temperature actuelle 2.7 Kelvin. Decouvert dans les annees soixante, et largement etudie depuis pour ses infimes fluctuations de temperature et de polarisation, il se propage librement depuis une epoque situee environ 400000 ans apres le big-bang. Il nous renseigne sur l'etat de l'Univers primordial, sur son contenu, ainsi que sur certains processus physiques ayant eu lieu ulterieurement, comme la reionisation ou la formation des amas de galaxie. Un autre rayonnement d'importance cosmologique a ete decouvert il y a seulement quatorze ans dans les donnees du satellite COBE, mais ici dans le domaine de l'infrarouge lointain, vers 200 microns de longueur d'onde: le {\bf rayonnement fossile des galaxies}, ou fond diffus extragalactique infrarouge. Il est environ 50 fois moins intense que le fond cosmologique (pic a pic, ou de 20 a 40 fois moins en integrale). Cette detection a constitue une grande surprise vu la grande intensite relative de l'infrarouge lointain par rapport a la partie visible provenant directement des etoiles. Ce fond diffus a pour origine l'emission de toutes les galaxies depuis leur formation, et resume ainsi toute leur histoire. Il est donc permis d'ecrire, presque sans ironie, que {\bf la nuit n'est pas noire}, dans la mesure ou, si nos yeux (avec le concours d'une hypothetique fenetre atmospherique) etaient sensibles aux rayonnements infrarouge lointain, millimetrique et centimetrique, ils verraient une nuit brillante de rayonnements cosmologiques. Notons que la question de la nuit noire - connue sous le nom de paradoxe d'Olbers Cheseaux - est ancienne, fertile, et touche aux fondements de la description et la comprehension de notre Univers physique, de son origine et de son evolution. Mesurer precisement ce rayonnement de fond infrarouge et comprendre la nature des galaxies qui en sont a l'origine et leur evolution, constituent l'une des thematiques importantes de la cosmologie observationnelle d'aujourd'hui. Je detaille, dans ce manuscrit de HDR, quelques-unes de mes contributions a l'etude statistique des galaxies infrarouges, a la mesure et l'interpretation du rayonnement extragalactique, et esquisse quelques perspectives. Le document de la HDR -- soutenue le 21 septembre 2010 a l'Institut d'Astrophysique Spatiale, Orsay -- et ses annexes sont disponibles en ligne sur http://www.ias. u-psud.fr/dole/hdr/
802.11G WLAN modeling for real time packet communication

This work proposes a statistical model of packet arrival and loss for wireless indoor environments in infrastructure mode. Usually, a Markov model with two states (Gilbert-Elliott, 1965) is used to model random processes of erroneous bits or packets. If we consider an equivalent model without memory, i.e. a random variable representing the burst length, then the Gilbert-Elliott model has a geometric distribution. We propose a statistical model for burst lengths of received and lost real time packets based on the logarithmic series distribution. We make use of a goodness-of-fit chi-square test to prove that our model surpasses the Gilbert-Elliott for 802.11g channels. Despite our model has been tested in a particular wireless scenario, it could also model other communications systems.
Diversity-Multiplexing Tradeoff with Arbitrary Doppler Spectrum

The original diversity-multiplexing tradeoff work by Zheng and Tse assumes multi-input-multi-output (MIMO) systems with frequency flat, spatially independent, and temporally block fading. Several following works have extended the tradeoff to either frequency selective or spatially correlated channels. However, all of them assume that fadings are temporally block. Compared with block fading assumption, it is more realistic to model Rayleigh temporal fadings as a zero-mean stationary complex Gaussian random process . Correspondingly, its power spectral density (or Doppler spectrum) is a sufficient description of the random process, because the correlation between two arbitrary fadings can be calculated through the Fourier transform of the Doppler spectrum. The main contribution of this paper is to derive the diversity- multiplexing tradeoff in frequency flat single-input-single-output (SISO) channels with arbitrary Doppler spectrum. Moreover, the derivation process itself, which is based on the spike sharpening effect, may be extended to facilitate other more complicated diversity-multiplexing tradeoff works.
Effects of ensiling on in situ ruminal degradability and intestinal digestibility of corn forage

The effective degradability of dry matter (DM), crude protein (CP) and amino acids (AA), and the intestinal effective digestibility (IED) of DM and CP of a green forage corn (GC) and its silage (EC) were determined on freeze-dried samples using three rumen and duodenum cannulated wethers. Two rumen incubations with duplicate bags were performed for each feed. Rumen degradation was determined on one series of bags from each incubation. The other series was freeze-dried and used to determine IED using mobile nylon bags. Microbial contamination of rumen incubated residues (determined with 15N techniques) fitted exponential functions, which showed a greater microbial contribution in EC than in GC in the undegradable DM (18.6% vs. 13.5%) and CP (81.7% vs. 69.4%). Degradability was calculated considering the particle rumen outflow rate (kp : 0.056/h) of the EC (EDp) or additionally the rate of comminution and mixing (kc : 0.130/h) of these particles (EDcp). Ensiling increased EDp (9.33%, p < 0.01) or EDcp (5.30%, p = 0.062) of DM and was associated with losses of nitrogen and with large changes in the AA profile. It is necessary to correct the microbial contamination, because it represents 32.0% (GC) and 42.5% (EC) of the undegraded CP when using kp and kc . Ensiling caused higher degradabilities for some AA as well as large differences in the changes due to the rumen fermentation on the AA profile. However, it had only limited effects on the undegraded protein profile. Ensiling also reduced the IED of DM (23.3% vs. 14.6%; p = 0.057). In conclusion, results do not show losses of nutritive value by ensiling corn cut at vitreous grain stage.
Argument Contained Ellipsis Revisited

This paper investigates an unusual identity constraint on English verb phrase ellipsis which imposes the following requirement: when an elliptical relation holds between two verb phrases A and B such that A is contained in an argument b of B, then the corresponding argument a of A must be identical to b. I argue that these facts provide evidence for a semantic theory of the interpretation of variables in which assignment functions are partial, and binding involves adding new variable/value pairs to an assignment, rather than reassigning new values to (possibly previously used) variables. Based on additional data, I argue that the identity conditions on ellipsis must be stated in terms of semantic parallelism between constituents (potentially) larger than the elided phrases, as advocated in Rooth 1992; Fox 1999a; Merchant 2001, and that the syntactic representation of binding structures must crucially encode binding indices on binders, rather than as adjuncts on their scope as in Heim and Kratzer 1998. I conclude by demonstrating that the analysis proposed here has broader empirical coverage than current variable-free analyses, and so potentially constitutes an argument for adopting a semantic framework that makes use of variables.
Human auditory steady-state responses: Respuestas auditivas de estado estable en humanos

Steady-state evoked potentials can be recorded from the human scalp in response to auditory stimuli presented at rates between 1 and 200 Hz or by periodic modulations of the amplitude and/or frequency of a continuous tone. Responses can be objectively detected using frequency-based analyses. In waking subjects, the responses are particularly prominent at rates near 40 Hz. Responses evoked by more rapidly presented stimuli are less affected by changes in arousal and can be evoked by multiple simultaneous stimuli without significant loss of amplitude. Response amplitude increases as the depth of modulation or the intensity increases. The phase delay of the response increases as the intensity or the carrier frequency decreases. Auditory steady-state responses are generated throughout the auditory nervous system, with cortical regions contributing more than brainstem generators to responses at lower modulation frequencies. These responses are useful for objectively evaluating auditory thresholds, assessing suprathreshold hearing, and monitoring the state of arousal during anesthesia. Los potenciales evocados de estado estable pueden registrarse del cráneo humano en respuesta a estímulos auditivos presentados a tasas de 1 y 200 Hz o por modulaciones periódicas de la amplitud y/o de la frecuencia de un tono continue Las respuestas pueden ser detectadas objetivamente por medio de un análisis frecuencial En sujetos en estado de alerta las respuestas son particularmente prominentes con tasas de estimulación cercanas a 40 Hz. Las respuestas evocadas por estímulos presentados a tasa más rápida resultan menos afectadas por cambios del estado de conciencia y pueden ser evocados por estímulos múltiples simultáneos sin una pérdida significativa de la amplitud. La amplitud de la respuesta aumenta conforme la profundidad de la modulación o de la intensidad aumenta. El retraso de fase de la respuesta aumenta conforme la intensidad de la frecuencia portadora aumenta. Las respuestas auditivas de estado estable se generan a todo lo largo del sistema nervioso auditivo; las regiones corticales contribuyen más que los generadores del tallo cerebral en las respuestas de frecuencias más bajas. Estas respuestas son útiles para evaluar objetivamente los umbrales de audición y permiten también evaluar la audición supraliminar y monitorizar el estado de conciencia durante la anestesia.
Location Based Anonymous Routing Protocol for MANETS

Privacy is needed in ad hoc networks. In order to provide anonymous routing protocol in MANETs the location of source and destination can be concealed by using “notify and go” mechanism and broadcasting. In this paper, we address some of the issues arising in MANETs by using an anonymous routing protocol. Location Based Anonymous Routing Protocol partitions the network into zones for anonymity protection. The destination position is identified to separate the source node from destination node by clustering, which is needed by the packet forwarder to check whether it is separated from the destination. The packet which is forwarded from source to destination can be encrypted by using asymmetric encryption algorithm. Location Based Anonymous Routing Protocol forwards a packet to the neighbor to the destination through the randomly selected relay nodes. Thus, it provides anonymous protection to source, destination, and routes at a low cost. It takes advantage of geographic routing protocol and also provides reliable communication. For routing anonymity temporary destination can be randomly chosen in other zone and relay nodes are selected in the same zone to transfer the data to temporary destination. Owe to the vulnerable nature of the mobile ad hoc network, there are various security threats that disturb the development of it. Location Based Anonymous Routing Protocol also has strategies to effectively counter the intersection and timing attacks.
Circulation of Infectious Bronchitis Virus Strains from Italy 02 and QX Genotypes in Slovenia Between 2007 and 2009

SUMMARY. Fourteen infectious bronchitis viruses (IBVs) detected in broiler, broiler breeder, and layer flocks in Slovenia between 2007 and 2009 were molecularly analyzed by reverse transcription–PCR and direct sequencing of the S1 gene. Phylogenetic analyses based on partial S1 gene sequences revealed that seven strains were classified as the Italy 02 genotype, a genetic group of IBV that has not previously been detected in Slovenia. Another seven strains were classified as the QX genotype. The results of phylogenetic analyses and epidemiologic investigations revealed that the genetic classification correlates with the geographic origins of the IBV strains. Greater genetic variability (maximum nucleotide and amino acid divergences were 4.8% and 9.9%, respectively) was observed within the Slovenian strains from the Italy 02 genotype detected between 2007 and 2009 than within strains from the QX genotype isolated in 2008 and 2009 (1.2% and 1.3%, respectively). The Slovenian strains classified as the Italy 02 genotype form a separate genetic cluster. These strains shared five specific amino acid substitutions that became fixed during the period of surveillance. Strains from the QX genotype that shared one specific amino acid substitution also form a separate genetic cluster.