Miracle-dz/UniFAR-FTUs huggingface dataset:
Knee joint loading differs in individuals with mild compared with moderate medial knee osteoarthritis.

OBJECTIVE To compare the knee joint loading patterns in individuals with differing radiographic grades of knee osteoarthritis (OA) for characterization of the mechanical implications of different structural states, and to compare the knee adduction angular impulse, a measure of gait complementary to the commonly used peak knee adduction moment. METHODS Asymptomatic subjects (those without knee OA) having a Kellgren/Lawrence (K/L) radiographic severity grade of 0 or 1 (n = 28) and subjects with symptomatic knee OA having K/L grades of 2 (n = 66) or 3 (n = 23) were recruited. Gait analysis was used to calculate the peak external knee adduction moment and the external knee adduction angular impulse for the whole stance and for the 4 subdivisions of stance. RESULTS Both the peak knee adduction moment and the knee adduction angular impulse increased with K/L radiographic grade (P < 0.05). However, only the knee adduction angular impulse differed between subjects with moderate (grade 3) and those with mild (grade 2) radiographic knee OA (P < 0.05). CONCLUSION The differences between mild and moderate symptomatic radiographic knee OA are not only structural but also functional, based on the magnitude of load in the medial knee joint. Moreover, knee adduction angular impulse provides additional information beyond that available from the peak knee adduction moment, and may therefore be an important gait parameter to include in OA research. These findings are important for our understanding of the pathophysiologic mechanisms of OA.
Ethylene Responses in Rice Roots and Coleoptiles Are Differentially Regulated by a Carotenoid Isomerase-Mediated Abscisic Acid Pathway[OPEN]

Ethylene induces the expression of MHZ5, a carotenoid isomerase, the production of neoxanthin, an ABA biosynthesis precursor, and the accumulation of ABA, leading to root growth inhibition in dark-grown rice seedlings. Ethylene and abscisic acid (ABA) act synergistically or antagonistically to regulate plant growth and development. ABA is derived from the carotenoid biosynthesis pathway. Here, we analyzed the interplay among ethylene, carotenoid biogenesis, and ABA in rice (Oryza sativa) using the rice ethylene response mutant mhz5, which displays a reduced ethylene response in roots but an enhanced ethylene response in coleoptiles. We found that MHZ5 encodes a carotenoid isomerase and that the mutation in mhz5 blocks carotenoid biosynthesis, reduces ABA accumulation, and promotes ethylene production in etiolated seedlings. ABA can largely rescue the ethylene response of the mhz5 mutant. Ethylene induces MHZ5 expression, the production of neoxanthin, an ABA biosynthesis precursor, and ABA accumulation in roots. MHZ5 overexpression results in enhanced ethylene sensitivity in roots and reduced ethylene sensitivity in coleoptiles. Mutation or overexpression of MHZ5 also alters the expression of ethylene-responsive genes. Genetic studies revealed that the MHZ5-mediated ABA pathway acts downstream of ethylene signaling to inhibit root growth. The MHZ5-mediated ABA pathway likely acts upstream but negatively regulates ethylene signaling to control coleoptile growth. Our study reveals novel interactions among ethylene, carotenogenesis, and ABA and provides insight into improvements in agronomic traits and adaptive growth through the manipulation of these pathways in rice.
Incompatibility and Mental Fatigue

A straightforward prediction from attention restoration theory is that the level of incompatibility in a person’s life should be positively correlated with that person’s level of mental (or directed attention) fatigue. The authors tested this prediction by developing a new self-report measure of incompatibility in which they attempted to isolate all the six categories of incompatibility described by S. Kaplan: distraction, deficit of information, duty, deception, difficulty, and danger. Factor analysis revealed six factors that corresponded reasonably well to those six categories. This article found that a composite incompatibility score was positively correlated with a separate self-report measure of mental fatigue and so were all six of the subscale scores. With the exception of the score for duty, these positive correlations remained after partialing out a separate measure of the level of stress in the person’s life. The authors concluded that the proposed categories of incompatibility can be validly measured; that the constructs of incompatibility, mental fatigue, and stress are discriminable from each other; and that incompatibility is generally positively correlated with mental fatigue.
Image classification using local binary pattern operators for static images

This paper aims to present an image classification method using Local Binary Pattern techniques. Local Binary Pattern operator transforms an static image, at pixel level, into a matrix of labels. These labels - integer numbers - describe and characterise the original image at a much lower scale. The authors propose the use of labels as a global characteristic of an static image. These techniques can be applied to an image or to a group of images and the characterization is done through an array of values extracted by the algorithm. The application developed allows the characterization of an image or a set of images, determining the similarity between different images and the degree of belonging to a particular group. Vectors of values are required for more images and image groups and each vector is representing different textures and their classification. As a result it becomes possible that indexing images, take into account the content of the information present in the image.
The BAsAS Architecture For Semantic Web Annotations

We describe a generic architecture for the (semi-automatic) creation, storage and querying for annotations of web resources. Our BAsAS architecture uses recent advances from the Semantic Web and Web 2.0 communities to make Semantic Web annotations a reality. The BAsAS architecture makes it easy for users to start to annotate and easy for developer to use the annotations that get created. Besides describing the general architecture we will also detail an implementation of this architecture build for a Semantic Web community portal.
Apnoea associated with the intrathecal administration of morphine in obstetrics. A case report.

The intrathecal administration of hyperbaric morphine 1 mg for pain relief during labour was followed, 7 h after injection, and 1 h after delivery, by respiratory depression. Pulseoximetry was helpful in alerting the medical personnel. After an initial 1 mg of naloxone i.v. in increments of 0.1-0.2 mg, a continuous infusion of naloxone 0.4 mg h-1 i.v. was used to prevent a recurrence of the respiratory depression. The total dose of naloxone over an 8-h period was 3.6 mg.
RAWLSIAN INVESTMENT RULES FOR “ INTERGENERATIONAL EQUITY “ : BREACHES OF METHOD AND ETHICS

HARVARD PROFESSOR OF PHILOSOPHY, John Rawls, can be credited with provoking the most recent angst over the issue of intergenerational equity. Rawls views intergenerational equity as a policy issue necessitating governmental intervention to manage “social saving and investment” for the benefit of the least advantaged generation. Economists John Hartwick and Robert Solow credit John Rawls’s treatise, A Theory of Justice, as the inspiration for their investment rules aimed at achieving intergenerational equity. For Rawls, saving and investing are equitable if these political judgments and actions increase or enhance the “welfare” of the least well-off generation of people (Rawls 1971, pp. 284–93). 1 Hartwick and Solow, however, address this Rawlsian concern not by focusing on the needs of the least favored generation, but rather by prescribing investment rules that assure each future generation a nondeclining level of per capita
The Caenorhabditis elegans rol-6 gene, which interacts with the sqt-1 collagen gene to determine organismal morphology, encodes a collagen

The rol-6 gene is one of the more than 40 loci in Caenorhabditis elegans that primarily affect organismal morphology. Certain mutations in the rol-6 gene produce animals that have the right roller phenotype, i.e., they are twisted into a right-handed helix. The rol-6 gene interacts with another gene that affects morphology, sqt-1; a left roller allele of sqt-1 acts as a dominant suppressor of a right roller allele of rol-6. The sqt-1 gene has previously been shown to encode a collagen. We isolated and sequenced the rol-6 gene and found that it also encodes a collagen. The rol-6 gene was identified by physical mapping of overlapping chromosomal deficiencies that cover the gene and by identification of an allele-specific restriction site alteration. The amino acid sequence of the collagen encoded by rol-6 is more similar to that of the sqt-1 collagen than to any of the other ten C. elegans cuticle collagen sequences compared. The locations of cysteine residues flanking the Gly-X-Y repeat regions of rol-6 and sqt-1 are identical, but differ from those in the other collagens. The sequence similarities between rol-6 and sqt-1 indicate that they represent a new collagen subfamily in C. elegans. These findings suggest that these two collagens physically interact, possibly explaining the genetic interaction seen between the rol-6 and sqt-1 genes.
CEACAM1 is a Privileged Cell Surface Antigen to Design Novel ScFv Mediated-Immunotherapies of Melanoma, Lung Cancer and Other Typesof Tumors

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a cell surface glycoprotein involved in intercellular binding, belonging to the immunoglobulin superfamily. It is involved in cell-cell recognition and modulates cellular processes that range from vascular angiogenesis to the regulation of insulin homeostasis and T-cell proliferation. Aberrant expression of CEACAM1 is often associated with progression and metastatic potential in melanoma, lung carcinoma and other types of tumor. Tumor-specific antigens such as CEACAM1 are ideal targets for cancer immunotherapy because they are over-expressed by the cancer cell and not on non-malignant tissues, minimizing the risk of autoimmune destruction. Many of the limitations of therapeutic use of rodent monoclonal antibodies (mAbs) can now be overcome by exploiting the use of recombinant antibody fragments and the advances in antibody engineering methods to improve tumor retention, reduce immunogenicity and modulate pharmacokinetics. In addition, a novel effective model of immunotherapeutic treatments of tumors includes antibody drug conjugates (ADCs) that combine specific mAbs and antibody fragments with cytotoxic drugs, proteins, enzymes, radionuclides and nanoparticles. This review aims to describe how these antibody engineering approaches can meet the challenges for generating new and effective antibody constructs for diagnosis and therapy of CEACAM1 expressing malignancies.
Characterization of microsatellites in the mountain plant Armeria caespitosa (Plumbaginaceae) and transferability to congeners.

PREMISE OF THE STUDY The focus of this study is to develop microsatellite markers in Armeria caespitosa, a narrow endemic of central Spain. Microsatellite loci are sought to clarify population structure and estimate gene flux among populations. METHODS AND RESULTS Enriched microsatellite genomic libraries were used for microsatellite isolation. Sixteen microsatellite loci were characterized, eight of which can be used for successful genotyping. Allele number ranged from two to seven per locus. Observed and expected heterozygosity ranged from 0.300 to 0.800 and from 0.296 to 0.733, respectively. Cross-amplification of seven and six loci was successful for A. maritima and A. cantabrica, respectively. CONCLUSIONS These microsatellites are suitable in the study of population genetics and gene flow among A. caespitosa populations. The information provided by these markers may be useful in the study of this plant's response to global warming.
A traffic assignment model for a ridesharing transportation market

Summary A nascent ridesharing industry is being enabled by new communication technologies and motivated by the many possible benefits, such as reduction in travel cost, pollution, and congestion. Understanding the complex relations between ridesharing and traffic congestion is a critical step in the evaluation of a ridesharing enterprise or of the convenience of regulatory policies or incentives to promote ridesharing. In this work, we propose a new traffic assignment model that explicitly represents ridesharing as a mode of transportation. The objective is to analyze how ridesharing impacts traffic congestion, how people can be motivated to participate in ridesharing, and, conversely, how congestion influences ridesharing, including ridesharing prices and the number of drivers and passengers. This model is built by combining a ridesharing market model with a classic elastic demand Wardrop traffic equilibrium model. Our computational results show that (i) the ridesharing base price influences the congestion level, (ii) within a certain price range, an increase in price may reduce the traffic congestion, and (iii) the utilization of ridesharing increases as the congestion increases. Copyright © 2014 John Wiley & Sons, Ltd.
Intra- and Intermonomer Interactions Are Required to Synergistically Facilitate ATP Hydrolysis in Hsp90*S⃞♦

Nucleotide-dependent conformational changes of the constitutively dimeric molecular chaperone Hsp90 are integral to its molecular mechanism. Recent full-length crystal structures (Protein Data Bank codes 2IOQ, 2CG9, AND 2IOP) of Hsp90 homologs reveal large scale quaternary domain rearrangements upon the addition of nucleotides. Although previous work has shown the importance of C-terminal domain dimerization for efficient ATP hydrolysis, which should imply cooperativity, other studies suggest that the two ATPases function independently. Using the crystal structures as a guide, we examined the role of intra- and intermonomer interactions in stabilizing the ATPase activity of a single active site within an intact dimer. This was accomplished by creating heterodimers that allow us to differentially mutate each monomer, probing the context in which particular residues are important for ATP hydrolysis. Although the ATPase activity of each monomer can function independently, we found that the activity of one monomer could be inhibited by the mutation of hydrophobic residues on the trans N-terminal domain (opposite monomer). Furthermore, these trans interactions are synergistically mediated by a loop on the cis middle domain. This loop contains hydrophobic residues as well as a critical arginine that provides a direct linkage to the γ-phosphate of bound ATP. Small angle x-ray scattering demonstrates that deleterious mutations block domain closure in the presence of AMPPNP (5′-adenylyl-β,γ-imidodiphosphate), providing a direct linkage between structural changes and functional consequences. Together, these data indicate that both the cis monomer and the trans monomer and the intradomain and interdomain interactions cooperatively stabilize the active conformation of each active site and help explain the importance of dimer formation.
Ceftobiprole- and Ceftaroline-Resistant Methicillin-Resistant Staphylococcus aureus

ABSTRACT The role of mecA mutations in conferring resistance to ceftobiprole and ceftaroline, cephalosporins with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, was determined with MRSA strains COL and SF8300. The SF8300 ceftaroline-passaged mutant carried a single mecA mutation, E447K (E-to-K change at position 447), and expressed low-level resistance. This mutation in COL conferred high-level resistance to ceftobiprole but only low-level resistance to ceftaroline. The COL ceftaroline-passaged mutant, which expressed high-level resistance to ceftobiprole and ceftaroline, had mutations in pbp2, pbp4, and gdpP but not mecA.
Training Parents in Child Behavior-Management Skills: A Group Approach

Parent training as a valued treatment and prevention service has a long history in the United States of America. In the last decade, however, parent training has become much more popular in the fields of child mental health and child welfare. As a treatment concept, the focus on training of skills-and not necessarily on the modification of traits, basic predispositions, or the exposition of underlying conflicts-offers a model of the therapist as a sensitive educator rather than a "kind of detective. "' As a primary prevention concept, parent training is an intervention designed to prevent or reduce the incidence of child abuse. As a mental health promotion concept, it is advantageous to the whole society, and not culture-bound to majority and middle-class concerns.2 Communication and reinforcement skills which previously were considered the tools of professional helpers only are now taught to parents, with positive results obtained from their application of these skills.3 In relation to general utility, techniques used to manage behavior have been shown to be effective over a wide range of behaviors,4
GABA-induced inactivation of functionally characterized sites in cat visual cortex (area 18): effects on orientation tuning

Microiontophoresis of the inhibitory transmitter GABA was used to reversibly inactivate small sites of defined orientation specificity at a horizontal distance of some 600 microns from single cells recorded in area 18 of cat visual cortex, and the effects on orientation tuning were studied. The receptive fields of cells at the recording and inactivation sites overlapped extensively. During the inactivation of sites where the orientation preference differed by 45 degrees or more from that of a recorded cell (“cross-orientation” sites), 65% of 54 cells tested showed significant broadening of orientation tuning, with a mean increase in tuning width (measured at half the maximum response) of 93%, and an almost fourfold increase in the relative response to the orientation orthogonal to the optimum, compared with the response to the optimum; four cells essentially lost their orientation tuning. Broadening of tuning reflected an increase in response to nonoptimal orientations and was reversible upon termination of GABA application. The effects on orientation tuning typically peaked within 10–15 min of the onset of GABA iontophoresis with 50–100 nA ejecting currents, and could not be replicated by inactivating sites where the orientation preference was similar to that of a recorded cell; when the orientation preference at the inactivation sites was within 22.5 degrees of that of a recorded cell (“iso-orientation” sites), only 3 of 22 cells showed significant broadening of tuning, and in these cases, the effects were relatively weak (mean increase in tuning width of 39% and a negligible change in the relative response to the orientation orthogonal to the optimum). The effect of inactivating “iso-orientation” sites consisted primarily in an increase in response magnitude. The difference in the magnitude of the effects on orientation tuning elicited by inactivating “cross-orientation” and “iso-orientation” sites was highly statistically significant. Additionally, inactivation of “cross- orientation” or “iso-orientation” sites elicited differential effects on orientation tuning in 10 of the 13 cells in which direct comparisons were made. It is argued that the observed broadening of tuning was due to the loss of a “cross-orientation” inhibitory input, which normally sharpens orientation tuning by suppressing responses to nonoptimal orientations.
The approximability of MAX CSP with fixed-value constraints

In the maximum constraint satisfaction problem (MAX CSP), one is given a finite collection of (possibly weighted) constraints on overlapping sets of variables, and the goal is to assign values from a given finite domain to the variables so as to maximize the number (or the total weight, for the weighted case) of satisfied constraints. This problem is NP-hard in general, and, therefore, it is natural to study how restricting the allowed types of constraints affects the approximability of the problem. In this article, we show that any MAX CSP problem with a finite set of allowed constraint types, which includes all fixed-value constraints (i.e., constraints of the form x = a), is either solvable exactly in polynomial time or else is APX-complete, even if the number of occurrences of variables in instances is bounded. Moreover, we present a simple description of all polynomial-time solvable cases of our problem. This description relies on the well-known algebraic combinatorial property of supermodularity.
Effects of spironolactone on atrial structural remodelling in a canine model of atrial fibrillation produced by prolonged atrial pacing

Background and purpose: Suppression of the renin‐angiotensin‐aldosterone system can prevent atrial fibrillation (AF) by attenuating atrial structural remodelling but the role of aldosterone in AF prevention has not been investigated thoroughly. We explored whether the aldosterone antagonist, spironolactone, could improve atrial structural remodelling in long‐term rapid pacing‐induced AF.
NERGY E FFICIENCY IN A D H OC N ETWORKS

Wireless Ad Hoc Networks comprise a fast developing research area with a vast spectrum of applications. Wireless sensor network systems enable the reliable monitoring of a variety of environments for both civil and military applications. The Energy efficiency continues to be a key factor in limiting the deployability of ad-hoc networks. Deploying an energy efficient system exploiting the maximum lifetime of the network has remained a great challenge since years. The time period from the instant at which the network starts functioning to the time instant at which the first network node runs out of energy, i.e. the network lifetime is largely dependent on the system energy efficiency. In this paper, we look at energy efficient protocols, which can have significant impact on the lifetime of these networks. The cluster heads get drain out maximum energy in the wireless ad hoc networks. We propose an algorithm that deals with minimizing the rate of dissipation of energy of cluster heads. The algorithm LEAD deals with energy efficient round scheduling of cluster head allocation of nodes and then followed by allocation of nodes to the cluster heads maximizing network lifetime using ANDA [1, 2]. We compare our results with the previous works.