Miracle-dz/UniFAR-FTUs huggingface dataset:
Drug resistance gene amplification of plasmid NR1 derivatives with various amounts of resistance determinant DNA

Drug resistance gene amplification of derivatives of plasmid NR1 having various amounts of resistance (r) determinant DNA was examined with two types of NR1 derivatives. The first was an NR1 derivative that carried two tandem copies of the r determinant component which was isolated as an intermediate in the amplification process. The plating efficiency of host cells and restriction endonuclease analysis of the plasmid DNA indicate that plasmids with two tandem copies of the r determinant undergo spontaneous amplification to a more highly amplified state at a frequency 150-fold higher than that of wild-type NR1. The second class of derivatives consisted of plasmids in which different regions of the r determinant component had been deleted. The relationship between spontaneous amplification frequency and r determinant size was examined with these plasmids. Plating efficiency of host cells indicated that plasmids with a smaller r determinant undergo spontaneous amplification at a lower frequency than do plasmids with a larger r determinant. These results suggest that there is an ordered sequence of events in the amplification of the r determinant of NR1.
Sub-THz OOK receiver for chip to chip data link

The chip to chip data link receiver is implemented with the non-resonant plasma wave detector at 250 GHz. The receiver is composed with on-chip patch antenna, stacked FET detector, and limiting amplifier. The stacked FET detector detect THz signal by non-resonant plasma wave transistor. The non-resonant plasma wave transistor is based on the broadband direct detection well beyond the cutoff frequency of the technology. The on-chip patch antenna is designed by top metal and the bottom metal of the technology. The bandwidth of receiver is limited by baseband limiting amplifier. The limiting amplifier is designed by 5GHz bandwidth and 40dB voltage gain. The receiver is designed for 10Gbps on-off keying (OOK) communication. Demodulation at 4 kbps has been verified because of the limited modulation frequency of THz source. The chip to chip data link with non-resonant plasma wave transistor is firstly implemented by CMOS process.
Oxidative Balance Score, Colorectal Adenoma, and Markers of Oxidative Stress and Inflammation

Background: An oxidative balance score (OBS) that combines pro- and antioxidant exposures was previously reported to be associated with incident sporadic colorectal adenoma. We extend the previous analyses by assessing associations of the OBS and colorectal adenoma with circulating biomarkers of oxidative stress [F2-isoprostanes (FIP) and fluorescent oxidation products (FOP)], and inflammation [C-reactive protein (CRP)]. Methods: Using pooled data from two previously conducted colonoscopy-based case–control studies of incident, sporadic colorectal adenoma (n = 365), the OBS was constructed and divided into three approximately equal intervals, with the lowest interval used as the reference. Biomarker levels were dichotomized as “high” versus “low” based on the median values among controls. Multivariable logistic regression was used to calculate adjusted odds ratios (ORs) and 95% confidence intervals (CIs). Results: For the OBS–adenoma association, the ORs (95% CIs) for the middle and highest (relative to the lowest) score intervals were 0.81 (0.46–1.43) and 0.39 (0.17–0.89), respectively. The corresponding OBS category-specific ORs (95% CIs) were 0.50 (0.25–1.01) and 0.25 (0.10–0.65) for FIP, 2.01 (1.13–3.75) and 3.48 (1.51–8.02) for FOP, and 0.57 (0.31–1.04) and 0.21 (0.09–0.49) for CRP. The ORs (95% CIs) reflecting associations of adenoma with high levels of FIP, FOP, and CRP were 1.89 (1.08–3.30), 1.82 (1.11–2.99), and 1.45 (0.88–2.40), respectively. Conclusions: As hypothesized, the OBS was inversely associated with colorectal adenoma and circulating FIP and CRP levels. The reason for the unexpected direct OBS–FOP association is unknown. Impact: These data support the use of combined measures of pro- and antioxidant exposures in studies of colorectal neoplasia. Cancer Epidemiol Biomarkers Prev; 23(3); 545–54. ©2014 AACR.
On capacity of Rician MIMO channels

Use of antenna arrays at both sides of the communication link can result in high channel capacities provided that the propagation medium is rich scattering. Previous studies on MIMO wireless structures have mostly considered Rayleigh fading conditions. Here a study is made on the capacity of Rician channels; it is shown that depending on the number of antennas, the MIMO capacity achieved under the condition of Rician fading may be greater or smaller than the capacity in the case of Rayleigh fading. In particular, for a large number of antennas, Rician channels exhibit smaller capacity than Rayleigh channels. On the other hand, for Rician channels, if the line-of-sight component is not very significant, the increase in capacity by an increase in the number of antennas can still be considerable.
Impact of levosimendan on right ventricular functions by using novel tissue Doppler derived indices in patients with ischaemic left ventricular failure.

BACKGROUND Right ventricular (RV) dysfunction complicates advanced left ventricular (LV) heart failure (HF) and contributes to a poor prognosis. Levosimendan is a positive inotropic agent improving cardiac contractility without increasing myocardial oxygen consumption in HF. AIM To evaluate the effect of levosimendan therapy on RV systolic function, by using derived tissue Doppler imaging (TDI) in patients with ischaemic HF. METHODS The study consisted of 30 patients with HF who were admitted to our hospital due to new onset of decompensated HF, having LV ejection fraction < 40%, with functional status class III-IV, according to the New York Heart Association (NYHA). TDI-derived systolic velocities of tricuspid annulus (isovolumic myocardial acceleration [IVA], peak myocardial velocity during isovolumic contraction [IVV], peak systolic velocity during ejection period [Sa]) and diastolic indices (early [Ea] and late diastolic [Aa] velocities, Ea/Aa, E/Ea ratios and myocardial performance index [Tei index]) were measured. 72 hours after treatment, all measurements were re-evaluated. RESULTS Considering TDI-derived systolic indices of the RV, IVA and IVV increased significantly, whereas Tei index decreased, after the therapy. Also, there was a significant decrease in serum levels of B-type natriuretic peptide. No significant change was observed in TDI derived diastolic indices of the RV. CONCLUSIONS Levosimendan improves RV systolic function as expressed by TDI-derived parameters in patients with acutedecompensated HF.
The research on micro-grid mode conversion

Generation and associated loads were viewed as a subsystem or a “micro-grid”; micro-grid can improve the utilization of renewable energy and important load supply reliability. More and more countries began to strengthen the study of micro-grid, power and energy balance, power quality, protection and control are the important research objects of micro-grid. This article will focus on the micro-grid mode conversion control.
Onset and Offset as Determinants of the Simon Effect.

We investigated the presence and the characteristics of the Simon effect for onset and offset targets when these stimuli are randomly intermixed. In Experiment 1, two possible target locations were occupied by an occluder. On onset trials, a target appeared above an occluder, while on offset trials one of the occluders disappeared, revealing the target underneath. In Experiment 2, four stimuli appeared randomly in six possible locations. On onset trials, a new stimulus appeared in an empty location while on offset trials, one of the initial stimuli disappeared. In both experiments, the Simon effect for onset and offset targets was characterized by similar size, time course and sequential modulation, suggesting similar sensorimotor interactions between target and response locations. However, the Simon effect in the current trials was more evident when the same type of target (onset or offset) was repeated on successive trials demonstrating the role of stimulus category in its modulation.
Disordered Eating in African American and Caucasian Women: The Role of Ethnic Identity

The influential roles of culture and ethnic identity are frequently cited in developing disordered eating and body dissatisfaction, constituting both protective and risk factors. For African American women, strongly identifying with African American cultural beauty ideals may protect against disordered eating to lose weight, but may actually increase risk in development of disordered eating directed at weight gain, such as binge eating. This study compares African American and Caucasian women on disordered eating measures, positing that African American women show greater risk for binge eating due to the impact of ethnic identity on body dissatisfaction. Findings indicate low levels of ethnic identity represent a risk factor for African American women, increasing the likelihood of showing greater binge eating and bulimic pathology. In Caucasian women, high levels of ethnic identity constitute a risk factor, leading to higher levels of both binge eating and global eating pathology. Implications for prevention and treatment are discussed.
Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

ABSTRACT Dengue virus (DV) was detected early in infected mosquito C6/36 cells by using indirect immunofluorescence (IF) in conjunction with flow cytometry. Three fixation-permeabilization methods and three DV serotype 1 (DEN-1)-specific monoclonal antibodies, 8-8 (anti-E), 16-4 (anti-NS1), and 15F3-1 (anti-NS1), were evaluated for the detection of DEN-1 in infected C6/36 cells. We found that these three monoclonal antibodies were capable of detecting DV in C6/36 cells as early as 24 h postinoculation by using a conventional indirect IF stain. Both 8-8 and 16-4 detected DV earlier and showed a greater number of DV-positive cells than 15F3-1. In flow cytometry, 3% paraformaldehyde plus 0.1% Triton X-100 with 16-4, the best fixation-permeabilization method for testing DV, showed higher sensitivity (up to 1 PFU) than indirect IF stain. The higher sensitivity of 16-4 in detecting DEN-1 was found with both IF and flow cytometry. Flow cytometry, which had a sensitivity similar to that of nested reverse transcription-PCR, was more sensitive in detecting DV in the infected mosquito cells 10 h earlier than the conventional IF stain. When clinical specimens were amplified in mosquito C6/36 cells and then assayed for DV using flow cytometry and conventional virus isolation at day 7 postinfection, both methods had 97.22% (35 out of 36) agreement. Moreover, among 12 positive samples which were detected by conventional culture method, the flow cytometry assay could detect DV in 58.33% (7 out of 12) of samples even at day 3 postinfection. In conclusion, both monoclonal antibodies 8-8 and 16-4 can be used for the early detection of DEN-1-infected C6/36 cells, with 16-4 (anti-NS1) being the best choice for the rapid diagnosis of DV by both the IF staining and flow cytometry methods.
Chromatin remodeling inactivates activity genes and regulates neural coding

Epigenetic regulation in the brain The activity of neurons in the brain controls the transcription of genes that influence the pruning of dendritic connections between neurons, and such modifications can influence animal behavior. Yang et al. propose a role for chromatin remodeling by the nucleosome remodeling and deacetylase complex (NuRD) in the inactivation of such activity-dependent transcription in the mouse cerebellum (see the Perspective by Sweatt). Deposition of the histone variant H2A.z at promoters of activity-dependent genes required the NuRD complex. Loss of the NuRD complex function resulted in hypersensitivity of mice to sensory stimuli and excessive neuronal connectivity in animals performing a task on a treadmill. Science, this issue p. 300; see also p. 218 Epigenetic control of transcription influences neuronal connectivity. Activity-dependent transcription influences neuronal connectivity, but the roles and mechanisms of inactivation of activity-dependent genes have remained poorly understood. Genome-wide analyses in the mouse cerebellum revealed that the nucleosome remodeling and deacetylase (NuRD) complex deposits the histone variant H2A.z at promoters of activity-dependent genes, thereby triggering their inactivation. Purification of translating messenger RNAs from synchronously developing granule neurons (Sync-TRAP) showed that conditional knockout of the core NuRD subunit Chd4 impairs inactivation of activity-dependent genes when neurons undergo dendrite pruning. Chd4 knockout or expression of NuRD-regulated activity genes impairs dendrite pruning. Imaging of behaving mice revealed hyperresponsivity of granule neurons to sensorimotor stimuli upon Chd4 knockout. Our findings define an epigenetic mechanism that inactivates activity-dependent transcription and regulates dendrite patterning and sensorimotor encoding in the brain.
Power System Risk Assessment in Cyber Attacks Considering the Role of Protection Systems

This paper presents a risk assessment method for evaluating the cyber security of power systems considering the role of protection systems. This paper considers the impact of bus and transmission line protection systems located in substations on the cyber-physical performance of power systems. The proposed method simulates the physical response of power systems to malicious attacks on protection system settings and parameters. The relationship among settings of protection devices, protection logics, and circuit breaker logics is analyzed. The expected load curtailment (ELC) index is used in this paper to quantify potential system losses due to cyber attacks. The Monte Carlo simulation is applied to calculate ELC for assessing attackers’ capabilities as bus arrangements are altered. The effectiveness of the proposed risk assessment method is demonstrated using a 9-bus system and the IEEE 68-bus system.
Expression profiling in cancer using cDNA microarrays

Currently there are over 1000000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50‐90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power of the technology is the ability to perform a genome‐wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future application of this powerful technique.
A framework for service enterprise workflow simulation with multi-agents cooperation

Process dynamic modelling for service business is the key technique for Service-Oriented information systems and service business management, and the workflow model of business processes is the core part of service systems. Service business workflow simulation is the prevalent approach to be used for analysis of service business process dynamically. Generic method for service business workflow simulation is based on the discrete event queuing theory, which is lack of flexibility and scalability. In this paper, we propose a service workflow-oriented framework for the process simulation of service businesses using multi-agent cooperation to address the above issues. Social rationality of agent is introduced into the proposed framework. Adopting rationality as one social factor for decision-making strategies, a flexible scheduling for activity instances has been implemented. A system prototype has been developed to validate the proposed simulation framework through a business case study.
Hepatic glycogen storage disorders: what have we learned in recent years?

Purpose of reviewGlycogen storage disorders (GSDs) are inborn errors of metabolism with abnormal storage or utilization of glycogen. The present review focuses on recent advances in hepatic GSD types I, III and VI/IX, with emphasis on clinical aspects and treatment. Recent findingsEvidence accumulates that poor metabolic control is a risk factor for the development of long-term complications, such as liver adenomas, low bone density/osteoporosis, and kidney disease in GSD I. However, mechanisms leading to these complications remain poorly understood and are being investigated. Molecular causes underlying neutropenia and neutrophil dysfunction in GSD I have been elucidated. Case series provide new insights into the natural course and outcome of GSD types VI and IX. For GSD III, a high protein/fat diet has been reported to improve (cardio)myopathy, but the beneficial effect of this dietary concept on muscle and liver disease manifestations needs to be further established in prospective studies. SummaryAlthough further knowledge has been gained regarding pathophysiology, disease course, treatment, and complications of hepatic GSDs, more controlled prospective studies are needed to assess effects of different dietary and medical treatment options on long-term outcome and quality of life.
Persistent Renal Dysfunction After Percutaneous Coronary Intervention in Patients With Acute Myocardial Infarction

We determined the incidence, predictors, and outcomes of persistent renal dysfunction (PRD) following percutaneous coronary intervention (PCI) in patients with acute myocardial infarction (AMI). Among 16 264 patients enrolled in a nationwide registry, we studied patients with AMI who had their estimated glomerular filtration rate at baseline and 1 month later (n = 3606). We used multivariate regression and propensity score (PS)-matched Cox proportional hazards to evaluate the association between PRD and outcomes. Persistent renal dysfunction occurred in 1333 (37%) patients. Significant PRD contributors included old age, low body mass index (BMI), hypertension, Killip class, and the extent of vessel disease. Persistent renal dysfunction was associated with an increased 1-year major adverse cardiac events (all-cause death, myocardial infarction, or revascularization) relative to no-PRD (entire cohort: 6.2% vs 4.5%, hazard ratio[HR] 1.63, 95% confidence interval [CI] 1.18-2.25, P = .003; PS-matched cohort: 7.2% vs 4.9%, HR 1.67, 95% CI 1.08-2.58, P = .022). In conclusion, PRD occurred in approximately one-third of patients with AMI following PCI. It was associated with old age, hypertension, low BMI, initial hemodynamic instability, and extent of vessel disease and was a predictor of worse outcomes at 1 year.
Experimental fast quantum random number generation using high-dimensional entanglement with entropy monitoring

A quantum random number generator (QRNG) generates genuine randomness from the intrinsic probabilistic nature of quantum mechanics. The central problems for most QRNGs are estimating the entropy of the genuine randomness and producing such randomness at high rates. Here we propose and demonstrate a proof-of-concept QRNG that operates at a high rate of 24 Mbit/s by means of a high-dimensional entanglement system, in which the user monitors the entropy in real time via the observation of a nonlocal quantum interference, without a detailed characterization of the devices. Our work provides an important approach to a robust QRNG with trusted but error-prone devices.
Extracting reaction networks from databases–opening Pandora’s box

Large quantities of information describing the mechanisms of biological pathways continue to be collected in publicly available databases. At the same time, experiments have increased in scale, and biologists increasingly use pathways defined in online databases to interpret the results of experiments and generate hypotheses. Emerging computational techniques that exploit the rich biological information captured in reaction systems require formal standardized descriptions of pathways to extract these reaction networks and avoid the alternative: time-consuming and largely manual literature-based network reconstruction. Here, we systematically evaluate the effects of commonly used knowledge representations on the seemingly simple task of extracting a reaction network describing signal transduction from a pathway database. We show that this process is in fact surprisingly difficult, and the pathway representations adopted by various knowledge bases have dramatic consequences for reaction network extraction, connectivity, capture of pathway crosstalk and in the modelling of cell–cell interactions. Researchers constructing computational models built from automatically extracted reaction networks must therefore consider the issues we outline in this review to maximize the value of existing pathway knowledge.
Single phototube liquid scintillation counting of C14: application to an easily isolated derivative of blood glucose.

Conditions for efficient and reproducible liquid scintillation counting of C14 using a single phototube are described. The amplifier, scaler and power supply are the same as those used for the gas-phase proportional counting of C14O2 admixed with P-10 gas (9:1 argon-methane). A compound which will dissolve to the extent of about 10 mg/25 ml of xylene or 4:1 xylene-ethanol, which does not absorb light in the blue region and which does not quench phosphor scintillations can be assayed for C14 by this method with a sensitivity about equivalent to that of the gas-phase counting method which entails combustion of the compound to CO2. The counting efficiency in xylene is about 80% and in 4:1 xylene-ethanol about 70% of the C14 disintegrations occurring in the sample. The background counting rate is about 90 counts per minute. Glucose, isolated as the phenylosazone from a biological fluid, is converted to the glucosotriazole derivative. The derivative is held in solution, with the aid of H3BO3, in 25 ml of 4:1 xylene-ethanol containing 0.24% of the phosphor PBD (2-phenyl-5-(4 biphenylyl) 1,3,4-oxadizole). The standard deviation for individual samples in a specific activity determination, including the error in weighing the 10-mg sample of glucosotriazole derivative, is ±1.7% Submitted on July 2, 1956
Diversity of Sulfate-Reducing Bacteria in Oxic and Anoxic Regions of a Microbial Mat Characterized by Comparative Analysis of Dissimilatory Sulfite Reductase Genes

ABSTRACT Sequence analysis of genes encoding dissimilatory sulfite reductase (DSR) was used to identify sulfate-reducing bacteria in a hypersaline microbial mat and to evaluate their distribution in relation to levels of oxygen. The most highly diverse DSR sequences, most related to those of the Desulfonema-like organisms within the δ-proteobacteria, were recovered from oxic regions of the mat. This observation extends those of previous studies by us and others associating Desulfonema-like organisms with oxic habitats.
Evaluating Test Suite Effectiveness and Assessing Student Code via Constraint Logic Programming

A good suite of test inputs is an indispensable tool both for manual and automated assessment of student submissions to programming assignments. Yet, without a way to evaluate our test suites, it is difficult to know how well we are doing, much less improve our practice. We present a technique for evaluating a hand-generated test suite by comparing its ability to find defects against that of a test suite generated automatically using Constraint Logic Programming (CLP). We describe our technique and present a case study using student submissions for an assignment from a second-year programming course. Our results show that a CLP-generated test suite was able to identify significant defects that the instructor-generated suite missed, despite having similar code coverage.