Miracle-dz/UniFAR-FTUs huggingface dataset:
Exposure of HL-60 human leukemic cells to 4-hydroxynonenal promotes the formation of adduct(s) with (cid:11) -enolase devoid of plasminogen binding activity

4-Hydroxynonenal (HNE), the major product of lipoperoxidation, easily reacts with proteins through adduct formation between its three main functional groups and lysyl, histidyl, and cysteinyl residues of proteins. HNE is considered to be an ultimate mediator of toxic effects elicited by oxidative stress. It can be detected in several patho-physiological conditions, in which it affects cellular processes by addition to functional proteins. We demonstrated by mass spectrometry and confirmed by immunoblotting experiments the formation of HNE- α -enolase adduct(s) in HL-60 human leukemic cells. α -Enolase is a multifunctional protein that acts as a glycolytic enzyme, transcription factor (c-myc binding protein, MBP-1) and plasminogen receptor. HNE did not affect α -enolase enzymatic activity, expression, intracellular localization and did not change the expression and localization of MBP-1 either. Confocal and electronic microscopy results confirmed the plasmamembrane, cytosolic and nuclear localization of α -enolase in HL-60 cells and demonstrated that HNE was colocalized with α -enolase at the surface of cells, early after its addition. HNE caused a dose-and time-dependent reduction of the binding of plasminogen to α enolase. As a consequence, HNE reduced adhesion of HL-60 cells to endothelial cells (HUVECs). These results could suggest a new role for HNE in the control of tumour growth and invasion. nuclear adherent cells versus control value. Control adhesion was 49 ± 4 cells/microscope fields (n = 3). Data are the mean ± SD of three separate experiments.
Social class differentials in remarriage after divorce: results from a forward linkage study.

A forward linkage exercise is described in which a sample of over one thousand couples [in England and Wales] who divorced during 1979 were traced to see whether either or both partners remarried in the subsequent 2 1/2 years; for those who did the appropriate divorce and remarriage records were linked.One third 34 per cent of the divorced husbands and 33 per cent of the divorced wives remarried.These findings and the proportions of divorced husbands and wives who remarried according to their social class number of children age at previous marriage age at divorce and duration of marriage are presented and discussed. (EXCERPT)
Modulated phase-shifting for 3D scanning

We present a new 3D scanning method using modulated phase-shifting. Optical scanning of complex objects or scenes with significant global light transport, such as subsurface scattering, interreflections, volumetric scattering, etc. is a difficult task since the direct surface reflection will be mixed with the global illumination. The direct and global components can be effficiently separated using high frequency illumination which to some extend is done in traditional phase-shifting for 3D scanning. In this paper we introduce the concept of modulation based separation where a high frequency signal is multiplied on top of other signal. The modulated signal inherits the good separation properties of the high frequency signal and allows for removing artifacts due to global illumination. This technique can be used to clean up arbitrary projected signals, e.g. photographs as well as the sinusoid patterns used for phase-shifting. For the modulated phase-shifting, we propose a two-pass separation method exploiting high frequency patterns in two-dimensions that can filter out the global components much more completely than traditional one-pass separation methods. We demonstrate the effectiveness of our approach on a couple of scenes with significant subsurface scattering and interreflections.
Shades of Grey: An Interdisciplinary Approach to a Constructive Understanding of Diversity in the Communication Classroom

Most communication teachers hear students use offensive terms and unfairly characterize diverse people. Voicing about diversity in the classroom can be difficult and fearful. The easy solution is to side step hot-button topics. But students do not want to ignore difference, and classrooms versed in diversity are the foundation of a successful university education. This project asks the research question, “How can communication teachers help students to constructively understand diversity? ” This interdisciplinary approach first outlines the literature on teaching diversity and surveys established classroom methods. Second, the dual processing theory of human cognition and its relationship to prejudice and bias are discussed. Third, practical classroom solutions for shedding new light on diversity are implied. Last, a summary offers guidelines for managing classroom discussions about diversity, examines the limitations of this work, and offer suggestions for further inquiry.
Test Coverage of Data-Centric Dynamic Compositions in Service-Based Systems

This paper addresses the problem of integration testing of data-centric dynamic compositions in service-based systems. These compositions define abstract services, which are replaced by invocations to concrete candidate services at runtime. Testing all possible runtime instances of a composition is often unfeasible. We regard data dependencies between services as potential points of failure, and introduce the k-node data flow test coverage metric. Limiting the level of desired coverage helps to significantly reduce the search space of service combinations. We formulate the problem of generating a minimum set of test cases as a combinatorial optimization problem. Based on the formalization we present a mapping of the problem to the data model of FoCuS, a coverage analysis tool developed at IBM. FoCuS can efficiently compute near-optimal solutions, which we then use to automatically generate and execute test instances of the composition. We evaluate our prototype implementation using an illustrative scenario to show the end-to-end practicability of the approach.
Enhanced expression of brain interferon‐α and serotonin transporter in immunologically induced fatigue in rats

Immunologically induced fatigue was induced in rats by intraperitoneal injection of a synthetic double‐stranded RNA, polyriboinosinic : polyribocytidylic acid (poly I:C). An injection of poly I:C (3 mg/kg) decreased the daily amounts of spontaneous running wheel activity to ≈ 60% of the preinjection level until day 8. Quantitative analysis of mRNA levels demonstrated that interferon‐α (IFN‐α) and p38 mitogen‐activated protein kinase mRNAs increased in the medial preoptic, paraventricular and ventromedial hypothalamic nuclei and in cortex on both days 1 and 8, while interleukin‐1β and an inhibitor of nuclear factor κB (IκB)‐β mRNAs increased on day 1, but recovered within a week. Serotonin transporter (5‐HTT) mRNA also increased on days 1 and 8 after poly I:C injection in the same brain regions where IFN‐α mRNA increased. The increased 5‐HTT had a functional significance, because in vivo brain microdialysis revealed that an i.p. injection of poly I:C induced a decrease in the extracellular concentration of 5‐HT in the prefrontal cortex; the decrease was blocked by local perfusion with a nonselective 5‐HT reuptake inhibitor, imipramine. Finally, the poly I:C‐induced fatigue was attenuated by a 5‐HT1A receptor agonist but not by 5‐HT2, 5‐HT3 or dopamine D3 agonists. These findings, taken together, suggest that disorders in brain IFN‐α and 5‐HTT expression may be involved in the neuronal mechanisms of the poly I:C‐induced fatigue.
Systematic Status of Agehana elwesi f. cavaleriei Based on Morphological and Molecular Evidence

Agehana swallowtail butterflies are unusual in having 2 veins present in the broad lobe-like "tail" of their hindwing. Of the 2 species currently recognized in this genus, A. maraho is generally considered an endangered taxon endemic to Taiwan. The other species, A. elwesi, which is widespread in the southern part of continental China, contains a form superficially similar to A. maraho. This form is termed f. cavaleriei, and its distribution is restricted to southwestern China. The relationship between this form and A, maraho has not been critically examined using either morphology or molecular characters. The male genitalia and a 1530 bp fragment of mitochondrial DNA including the cytochrome oxidase I barcoding region suggest that f. cavaleriei is a form of A. elwesi rather than A. maraho.
Examining Relationships between Leisure Involvement, Psychological Commitment and Loyalty to a Recreation Agency

Despite the recent proliferation of leisure involvement and loyalty research, very little attention has been given to systematically conceptualize and examine the nature of leisure involvement's relationship with loyalty. The purpose of the present study was to examine whether psychological commitment intervenes in the relationship between fitness participants' leisure involvement and their behavioral loyalty to a recreation agency. Concomitant consideration was also given to the moderating effects of selected personal and social factors on this relationship (Iwasaki & Havitz, 1998). Comparisons of a Fully Mediated Model (F-M-M) with rival Direct Effects Models (D-E-M I & II), using structural equation modeling (SEM), suggested that psychological commitment mediated the effects of enduring involvement on patrons' behavioral loyalty (p <.01). That is, enduring involvement appeared to indirectly influence behavioral loyalty via a sequence of commitment factors. Significant evidence was also found for the direct effects of skill, motivation, social support, and social norms on enduring involvement, while some support was found for moderating effects (i.e., skill, motivation, social support, and side bets significantly moderated the effects of enduring involvement on commitment's formative factors). Overall, the findings of the study help uncover some of the key mechanisms/processes by which customers become loyal to a recreation agency. Marketing strategies may be developed to aim at strengthening customer loyalty by maximizing key antecedents of behavioral loyalty (i.e., enduring involvement and psychological commitment), as well as personal and social factors (e.g., skill, motivation, social support, social norms, and side bets/sunk costs).
Interpreting Gene-Expression Profiles in Transplantation: A Critical Appraisal

Oligonucleotide microarray technology has created a small revolution in the transplant community because it has helped to decipher previously unknown molecular processes involved in allograft pathology, redefined molecular patterns of diseases that are indistinguishable at the pathological level and made possible the definition of new prognostic factors for long-term graft outcomes. However, given the tremendous complexity of the biological processes that are involved in the pathology of a transplanted organ, the interpretation of transcriptomic data can be speculative and oversimplified. Here, we discuss critical considerations regarding the nature of the object studied by cDNA microarray technology, the means by which it is observed, the interpretation of the observations, and whether the observations make sense in the context of transplant-related scientific questions. Given these limitations, we believe that global approaches based on more functional biological intermediates are necessary for a better understanding of the molecular processes that regulate the physiopathology of the transplanted organ.
Associations between prospective symptom changes and slow-wave activity in patients with Internet gaming disorder

Abstract The identification of the predictive factors and biological markers associated with treatment-related changes in the symptoms of Internet gaming disorder (IGD) may provide a better understanding of the pathophysiology underlying this condition. Thus, the present study aimed to identify neurophysiological markers associated with symptom changes in IGD patients and to identify factors that may predict symptom improvements following outpatient treatment with pharmacotherapy. The present study included 20 IGD patients (mean age: 22.71 ± 5.47 years) and 29 healthy control subjects (mean age: 23.97 ± 4.36 years); all IGD patients completed a 6-month outpatient management program that included pharmacotherapy with selective serotonin reuptake inhibitors. Resting-state electroencephalography scans were acquired prior to and after treatment, and the primary treatment outcome was changes in scores on Young's Internet Addiction Test (IAT) from pre- to posttreatment. IGD patients showed increased resting-state electroencephalography activity in the delta and theta bands at baseline, but the increased delta band activity was normalized after 6 months of treatment and was significantly correlated with improvements in IGD symptoms. Additionally, higher absolute theta activity at baseline predicted a greater possibility of improvement in addiction symptoms following treatment, even after adjusting for the effects of depressive or anxiety symptoms. The present findings demonstrated that increased slow-wave activity represented a state neurophysiological marker in IGD patients and suggested that increased theta activity at baseline may be a favorable prognostic marker for this population.
Phonological awareness and early reading development in childhood apraxia of speech (CAS).

BACKGROUND Childhood apraxia of speech (CAS) is associated with phonological awareness, reading, and spelling deficits. Comparing literacy skills in CAS with other developmental speech disorders is critical for understanding the complexity of the disorder. AIMS This study compared the phonological awareness and reading development of children with CAS and children with inconsistent speech disorder (ISD). METHOD & PROCEDURES Participants included twelve children with CAS aged 4-7 years. Their performance was compared with twelve children with ISD (and normal speech motor planning) and twelve children with typical development on tasks measuring phonological awareness, letter-sound knowledge, real and non-word decoding, and access to underlying phonological representations of words. There was no significant difference in the age, gender, socio-economic status, and receptive vocabulary of the groups. The two groups with speech disorder were matched for severity and inconsistency of their speech impairment. OUTCOMES & RESULTS The results indicated that the CAS group had inferior phonological awareness than the ISD and typical development groups. The CAS group had a greater proportion of participants performing below their expected age level than the comparison groups on phonological awareness, letter-sound knowledge and decoding tasks. There was no difference in the performance of the CAS and ISD groups on the phonological representation task. CONCLUSIONS & IMPLICATIONS Children with CAS are particularly susceptible to phonological awareness and reading delay. Intervention for children with CAS must facilitate skills underlying reading development in addition to resolving speech deficits in order to improve the spoke and written language outcomes of this population.
Myristoylated Alanine‐Rich Protein Kinase Substrate (MARCKS) Regulates Small GTPase Rac1 and Cdc42 Activity and Is a Critical Mediator of Vascular Smooth Muscle Cell Migration in Intimal Hyperplasia Formation

Background Transcription of the myristoylated alanine‐rich C kinase substrate (MARCKS) is upregulated in animal models of intimal hyperplasia. MARCKS knockdown inhibits vascular smooth muscle cell (VSMC) migration in vitro; however, the mechanism is as yet unknown. We sought to elucidate the mechanism of MARCKS‐mediated motility and determine whether MARCKS knockdown reduces intimal hyperplasia formation in vivo. Methods and Results MARCKS knockdown blocked platelet‐derived growth factor (PDGF)‐induced translocation of cortactin to the cell cortex, impaired both lamellipodia and filopodia formation, and attenuated motility of human coronary artery smooth muscle cells (CASMCs). Activation of the small GTPases, Rac1 and Cdc42, was prevented by MARCKS knockdown. Phosphorylation of MARCKS resulted in a transient shift of MARCKS from the plasma membrane to the cytosol. MARCKS knockdown significantly decreased membrane‐associated phosphatidylinositol 4,5‐bisphosphate (PIP 2) levels. Cotransfection with an intact, unphosphorylated MARCKS, which has a high binding affinity for PIP 2, restored membrane‐associated PIP 2 levels and was indispensable for activation of Rac1 and Cdc42 and, ultimately, VSMC migration. Overexpression of MARCKS in differentiated VSMCs increased membrane PIP 2 abundance, Rac1 and Cdc42 activity, and cell motility. MARCKS protein was upregulated early in the development of intimal hyperplasia in the murine carotid ligation model. Decreased MARKCS expression, but not total knockdown, attenuated intimal hyperplasia formation. Conclusions MARCKS upregulation increases VSMC motility by activation of Rac1 and Cdc42. These effects are mediated by MARCKS sequestering PIP 2 at the plasma membrane. This study delineates a novel mechanism for MARCKS‐mediated VSMC migration and supports the rational for MARCKS knockdown to prevent intimal hyperplasia.
Gentian pengekstrakan mikro fasa pepejal tersalut poli(dimetilsiloksana)-poli(vinil alkohol) untuk analisis klorpirifos

Traditional liquid – liquid extraction of pesticides consumes large volumes of organic solvent which are hazardous to the operator and is not environment friendly. Solid phase microextraction (SPME) is a solvent less extraction method which is safer to the operator and is environmental friendly. A sol-gel hybrid fibre coating material, poly(dimethylsiloxane)-poly(vinyl alcohol) (PDMS-PVA) was synthesized and used in head space solid phase microextraction (HS-SPME) of chlorpyrifos. The thickness of the synthesised PDMS-PVA fiber coating was 13.5 μm and it is thermally stable up to 400 °C. The PDMS-PVA sol-gel hybrid fiber was also stable to two organic solvents tested; acetonitrile and dichloromethane (1 hour dipping) and showed no significant changes in extraction performance for chlorpyrifos. Extracted chlorpyrifos was analysed using gas chromatography electron capture detector (GC-ECD). Optimum SPME parameters affecting the PDMS-PVA HS-SPME performance namely extraction time (15 min), extraction temperature (50 °C), desorption time (5 min), desorption temperature (260 °C) and stirring rate (120 rpm) were used for extraction. It was found that HSSPME using PDMS-PVA sol-gel fiber gave significantly better extraction performance of chlorpyrifos compared to commercial 100 μm PDMS fiber. The PDMS-PVA fiber showed excellent operational performances such as temperature stability (up to 380 °C), coating lifetime (up to 170 times use) and organic solvent stability. The PDMS-PVA-HS-SPME method showed excellent recovery for chlorpyrifos from tomatoes (98.0 %, 5.9% RSD) at 0.01 μg/g spiked level (5 times lower than maximum residue limit set by European Union).
DoWitcher: Effective Worm Detection and Containment in the Internet Core

Enterprise networks are increasingly offloading the responsibility for worm detection and containment to the carrier networks. However, current approaches to the zero-day worm detection problem such as those based on content similarity of packet payloads are not scalable to the carrier link speeds (OC-48 and up-wards). In this paper, we introduce a new system, namely DoWitcher, which in contrast to previous approaches is scalable as well as able to detect the stealthiest worms that employ low-propagation rates or polymorphisms to evade detection. DoWitcher uses an incremental approach toward worm detection: First, it examines the layer-4 traffic features to discern the presence of a worm anomaly; Next, it determines a flow-filter mask that can be applied to isolate the suspect worm flows and; Finally, it enables full-packet capture of only those flows that match the mask, which are then processed by a longest common subsequence algorithm to extract the worm content signature. Via a proof-of-concept implementation on a commercially available network analyzer processing raw packets from an OC-48 link, we demonstrate the capability of DoWitcher to detect low-rate worms and extract signatures for even the polymorphic worms.
Cache-to-Cache: Could ISPs Cooperate to Decrease Peer-to-Peer Content Distribution Costs?

We consider whether cooperative caching may reduce the transit traffic costs of Internet service providers (ISPs) due to peer-to-peer (P2P) content distribution systems. We formulate two game-theoretic models for cooperative caching, one in which ISPs follow their selfish interests, and one in which they act altruistically. We show the existence of pure strategy Nash equilibria for both games, and evaluate the gains of cooperation on various network topologies, among them the AS level map of Northern Europe, using measured traces of P2P content popularity. We find that cooperation can lead to significant improvements of the cache efficiency with little communication overhead even if ISPs follow their selfish interests.
'Desperately' seeking research on agile requirements in the context of large-scale agile projects

In this paper, I propose an agenda for undertaking research on requirements engineering in the context of large-scale agile software development projects. In so doing, I draw from my own experience as a practitioner in large-scale agile software development projects, as well as a review of relevant literature. Based on this, the paper reflects on two central questions: what are the main challenges related to requirements in large-scale agile software development? And, what are the relevant themes for researching requirements in large-scale agile projects? The paper concludes by proposing some overall research questions for future research.
of the JSME Journal of Advanced Mechanical Design , Systems , and Manufacturing

The goal of this study is to investigate the effect of actuator position on the theoretical energy consumption of an electrically powered bipedal robot. Specifically, the study focuses on the legs of the afore-mentioned robot because their function is limited, as they are primarily used for either locomotion or standing, and thus, this is suitable for optimization. Furthermore, the hip and knee joints constitute the most powerful joints, and thus, these are studied first. A mathematical formulation is adopted to represent the relationship between the actuators and the robots joints. A genetic optimization is used to minimize the energy loss due to motor winding resistance and no-load torque by altering the position of the actuator with respect to the joints. The results indicate that the energy lost during typical motions can be reduced by up to 30% by using bi-articular actuation. Additionally, most benefits can be realized by adding only a bi-articular actuator between the hip roll and knee pitch axes. Ke words : Bipedal robots, Bi-articular actuation, Energy efficiency, Design optimization, Generic algorithms
Power allocation for mixed traffic broadcast with service outage constraint

To transmit a mixture of real-time and non-real-time traffic in a broadcast system, we impose a basic service rate r0 for real-time traffic and use the excess rate beyond r0 to transmit non-real-time traffic. Considering the time-varying nature of wireless channels, the basic service rate is guaranteed with a service outage constraint, where service outage occurs when the channel capacity is below the basic service rate. This approach is well suited for providing growing services like video, real-time TV, etc., in group transportation systems such as coach, high-speed train, and airplane. We show that the optimal power allocation policy depends only on the statistics of the minimum gain of all user channels, and it is a combination of water-filling and channel inversion. We provide the optimal power allocation policy, which guarantees that real-time traffic be delivered with quality of service (QoS) for every user. Moreover, we show that the required minimum average power to satisfy the service outage constraint increases linearly with the number of users.