Miracle-dz/UniFAR-FTUs huggingface dataset:
Establishment and Characterization of 7 New Monoclonal Antibodies to Tissue Inhibitor of Metalloproteinases-1

Tissue inhibitor of metalloproteinases-1 (TIMP-1) plays a pivotal role in tissue remodeling processes, such as inflammation, wound healing and cancer invasion. Experimental results have pointed to a role in angiogenesis, cell proliferation, apoptosis and in malignant transformation. In clinical investigations high tumor tissue or plasma levels of TIMP-1 have been shown to have a strong and independent association with shorter survival time for breast and colorectal cancer patients, respectively. The purpose of this study has been to develop and characterize new anti-TIMP-1 monoclonal antibodies that may be useful in future development of TIMP-1 immunoassays.Peptide-based epitope mapping reveals linear epitopes. Surface plasmon resonance was used to determine antibody affinity and ability of antibodies to sandwich with each other. Antigen recognition was tested using ELISA and a chemiluminescence microtiter immunoassay format. Three antibodies recognized linear peptides. Estimated antibody affinities for TIMP-1 ranged from 6.6 × 108 to>1010 1/M. Antibodies demonstrated different abilities in ‘capture’ and ‘detection’ positions in the sandwich experiment. All antibody pairs bound TIMP-1:ProMMP-9 complexes. TIMP-1:MMP-9 complexes were marginally reactive with five antibody pairs. The results suggest that the antibodies are unique. They may be useful in designing assays that recognize various forms of TIMP-1. Future studies will clarify whether the use of different combinations of antibodies will increase the clinical value of TIMP-1 measurements in the treatment of cancer patients.
Meeting Critical Security Objectives with Security-Enhanced Linux

Security-enhanced Linux incorporates a strong, flexible mandatory access control architecture into Linux. It provides a mechanism to enforce the separation of information based on confidentiality and integrity requirements. This allows threats of tampering and bypassing of application security mechanisms to be addressed and enables the confinement of damage that can be caused by malicious or flawed applications. Using the system’s type enforcement and role-based access control abstractions, it is possible to configure the system to meet a wide range of security needs. This paper describes how Security-enhanced Linux was used to meet a number of general-purpose system security objectives.
Interprofessional Education Activity Among Undergraduate Nursing and Pharmacy Students in the Middle East

Health care is increasingly complex and must draw upon the distinct, yet complementary skills of various health disciplines. Integration of health professionals as students through interprofessional education (IPE) is 1 way to promote early, and subsequently sustain, the principles of teamwork. A number of countries in the Middle East are undergoing rapid expansion in population and associated services for its residents. Over the past decade, Qatar has invested heavily in both health and educational infrastructure in commitment to develop its human capital. Domestic clinical education programs for various health care providers have been launched, including nursing and pharmacy. As these various curricula emerge, there is a unique opportunity to develop and incorporate interprofessional health education within this specific cultural context. We describe an IPE initiative between the nursing and pharmacy education programs in Qatar.
The shadow banking system: economic characteristics and regulatory issues

the shadow banking system, which the Financial Stability Board (FSB) has defined as credit intermediation involving activities and entities outside the regular banking system, was at the heart of the financial crisis of 2007/08. indeed, flawed securitization and the ensuing collapse of shadow banking entities such as Special investment vehicles (Sivs), Asset‐backed Commercial Paper Conduits (ABCP conduits) and also some money market Funds (mmFs) generated risk that spread through the whole financial system.
Diaschisis resulting from acute unilateral cerebral infarction. Quantitative evidence for man.

HOEDT-RASMUSSEN and Skinhoj 1,2 were the first to report bilateral reduction of hemispheric blood flow (HBF) in patients with unilateral cerebral infarction. They measured HBF by means of intracarotid injection of a bolus of radioactive inert gas and an uncollimated detector placed over each hemisphere and found the HBF to be reduced on the "healthy" side as well as in the diseased hemisphere. They did not measure cerebral metabolism but hypothesized that unilateral infarction reduced metabolism, and consequently, resulted in reduced blood flow in the contralateral hemisphere due to transneural depression. There were only six patients with unilateral cerebral infarction in their series, three of whom had thrombosis or embolic occlusion of the internal carotid artery. The smallness of their series was complicated further by the fact that one of the requirements of their method is that the bolus be injected into the internal carotid artery to avoid extracranial
Segmenting the Commercial Producer Market for Agricultural Inputs

A cluster analysis procedure was used to develop a market segmentation of U.S. crop and livestock farms with annual sales in excess of $100,000. The results indicate that four distinct segments exist: convenience buyers, balance buyers, price buyers, and performance buyers. Differences in preferences across these segments have important implications for the marketing strategies of agricultural input suppliers.
Discrimination Between Two Lung Diseases Using Chest Radiographs

Economic considerations make the conventional chest radiograph (X-ray) film an important ingredient in the diagnostic process. An initial clinical investigation for patients with suspected lung ailments is the study of the chest X-rays. The problem of detection for diseases in their early stages are well known using X-ray. A technique involving wavelets coefficient as the feature vector and Andrew's curve has been proposed for detection of mycobacterium tuberculosis (MTB). This paper presents new and important results whereby lung cancer (LC) may be detected and differentiated from MTB. A method to calculate misclassification probabilities is given
Circulation of DENV2 and DENV4 in Aedes aegypti (Diptera: Culicidae) mosquitoes from Praia, Santiago Island, Cabo Verde

Abstract Arthropod-borne viruses, such as Dengue (DENV), Chikungunya (CHIKV), and Zika (ZIKV), pose a challenge to public health, due to their worldwide distribution and large-scale outbreaks. Dengue fever is currently one of the most important diseases and it is caused by four serotypes of DENV and is mainly transmitted by the mosquito Aedes aegypti. It is estimated that 50–100 million cases are reported every year worldwide. More recently, CHIKV and ZIKV, which are also transmitted by Ae. aegypti, have caused epidemics in countries in the Caribbean region, the Pacific region, and Americas. Cabo Verde faced its first dengue outbreak in 2009, with more than 21,000 reported cases and four registered deaths. The epidemic was caused by DENV-3 transmitted by Ae. aegypti mosquitoes. In addition, the country faced a Zika outbreak with more than 7,500 notified cases from October 2015 to May 2016. In the present study, we conducted a survey in mosquito samples to detect arboviruses circulating in the local vector population. Collections were performed from November 2014 to January 2015, in the City of Praia, the capital of Cabo Verde, using aspirators and BG-sentinel traps. Samples were examined by multiplex Reverse Transcription-polymerase chain reaction. A total of 161 Ae. aegypti adult females were analyzed (34 pools) and from these samples, eight pools were found positive for DENV-2 and DENV-4. Our results revealed a very high natural infection rate in the vector population and showed two different serotypes co-circulating in the island that differ from the one detected in the 2009 outbreak in Cabo Verde.
Joint Ownership and Organizational Role: Critical Factors of IT-Driven Value

The concept of psychological ownership is used to enhance our understanding of the relationship between users and information technology professionals, and to describe the IT-business process relationship. We propose that the user-IT ownership interaction determines the role that IT professionals assume in the organization: partner, order taker, advisor or technocrat. Interviews at four major organizations support the existence of these roles, and indicate that the particular role assumed by the IT professionals is likely to be related to the value generated by IT. The study suggests that the highest IT value stems from partnership between the users and the individuals in the IT function, which requires both business process ownership by IT professionals and information system ownership by users.
The sexual behaviour of international travellers at two Glasgow GUM clinics

A survey of patients attending 2 Glasgow genitourinary medicine (GUM) clinics was conducted in 2 3-month periods in 1993 and 1994. Three hundred and twenty-five attendees who had travelled abroad in the preceding 3 months completed anonymous self-administered questionnaires about their sexual behaviour during these recent journeys abroad. There were 112 women and 213 men (185 heterosexuals and 28 homosexuals). Twenty-two (19.6%) women, 56 (31%) heterosexual men and 13 (42%) homesexual men had a sexual contact with a new partner while abroad. Of those who had had a new sexual contact abroad, 11 women (50% of those who had sex with a new partner) and 33 heterosexual men (59% of those who had sex with a new partner) were inconsistent users of condoms. Analysis of data found that homosexual and heterosexual men, and business travellers, are at increased risk of exposure to sexually transmitted diseases, including HIV infection, and should be targeted with safer sex health promotion prior to travel.
Effects of alcohol on exogenous precueing of attention.

OBJECTIVE Transient visual events cause a rapid allocation of attention to the event location. We studied the effect of alcohol on this aspect of attention. METHOD Subjects responded to targets presented either left or right of fixation and the time between target presentation and response (reaction time: RT) was measured both before and after ingestion of either alcohol or a placebo. Transient cues were presented prior to each target presentation. Cues were, variously, a brightening of either the box in which the target was to occur ("valid"), the box on the opposite side of the display from where the target was to occur ("invalid") or the fixation stimulus ("neutral"). The interval between cue and target presentation (stimulus onset asynchrony: SOA) was variously 50, 100, 150 or 800 msecs. RESULTS At short SOAs, RT was faster to targets presented following valid cues relative to the other cues. At 800 msecs the opposite pattern was found. Alcohol selectively slowed RT only following neutral or invalid cues at short SOAs. CONCLUSIONS Alcohol does not disrupt the normal attentional allocation to visual transients. It may delay the subsequent response to events at other locations, however.
Job Satisfaction of Primary Health-Care Providers (Public Sector) in Urban Setting

Introduction: Job satisfaction is determined by a discrepancy between what one wants in a job and what one has in a job. The core components of information necessary for what satisfies and motivates the health work force in our country are missing at policy level. Therefore present study will help us to know the factors for job satisfaction among primary health care providers in public sector. Materials and Methods: Present study is descriptive in nature conducted in public sector dispensaries/primary urban health centers in Delhi among health care providers. Pretested structured questionnaire was administered to 227 health care providers. Data was analyzed using SPSS and relevant statistical test were applied. Results: Analysis of study reveals that ANMs are more satisfied than MOs, Pharmacist and Lab assistants/Lab technicians; and the difference is significant (P < 0.01). Age and education level of health care providers don’t show any significant difference in job satisfaction. All the health care providers are dissatisfied from the training policies and practices, salaries and opportunities for career growth in the organization. Majority of variables studied for job satisfaction have low scores. Five factor were identified concerned with job satisfaction in factor analysis. Conclusion: Job satisfaction is poor for all the four groups of health care providers in dispensaries/primary urban health centers and it is not possible to assign a single factor as a sole determinant of dissatisfaction in the job. Therefore it is recommended that appropriate changes are required at the policy as well as at the dispensary/PUHC level to keep the health work force motivated under public sector in Delhi.
Time constraint route search over multi-locations

Abstract Traditional Route Search aims at finding the path that goes through geographical entities that are relevant to the provided search terms from the start point to the end point. Without constraints, traditional Route Search visiting multiple locations is unreliable because locations may close after a specified time. In this paper, time constraint (operating hours of each location) is drawn into Route Search query in order to make the query more realistic. Two methods are proposed in this paper, namely Route Search for fixed locations (RFix) and Route Search for flexible locations (RFlex). These two queries are different from the existing Route Search query because (1) the end point is not pre-defined and (2) time constraint is involved. Our two proposal queries consider whether the locations are specifically pre-defined by the user or only the location types are specified. In each method, two propositions are presented for pruning expansion branches, which highly improves the performance. Our experiments verified the applicability of RFix and RFlex to solve Route Search queries with time constraint queries.
CD70/CD27 signaling promotes blast stemness and is a viable therapeutic target in acute myeloid leukemia

Aberrant proliferation, symmetric self-renewal, increased survival, and defective differentiation of malignant blasts are key oncogenic drivers in acute myeloid leukemia (AML). Stem cell gene signatures predict poor prognosis in AML patients; however, with few exceptions, these deregulated molecular pathways cannot be targeted therapeutically. In this study, we demonstrate that the TNF superfamily ligand–receptor pair CD70/CD27 is expressed on AML blasts and AML stem/progenitor cells. CD70/CD27 signaling in AML cells activates stem cell gene expression programs, including the Wnt pathway, and promotes symmetric cell divisions and proliferation. Soluble CD27, reflecting the extent of CD70/CD27 interactions in vivo, was significantly elevated in the sera of newly diagnosed AML patients and is a strong independent negative prognostic biomarker for overall survival. Blocking the CD70/CD27 interaction by mAb induced asymmetric cell divisions and differentiation in AML blasts and AML stem/progenitor cells, inhibited cell growth and colony formation, and significantly prolonged survival in murine AML xenografts. Importantly, hematopoietic stem/progenitor cells from healthy BM donors express neither CD70 nor CD27 and were unaffected by blocking mAb treatment. Therefore, targeting CD70/CD27 signaling represents a promising therapeutic strategy for AML.
Tuning nanostructured morphology in polymers based on heterocyclic aromatic amines using phenyl amines as additive initiators for the construction of porous functional materials

In this work, the effect of monomer structures on the morphology of polymerized aromatic amines was investigated. Several polymerized aromatic amines were prepared by oxidative chemical using different oxidants. Besides, the synthesis of those polymers was attempted using p-phenylenediamine or p-amino-diphenylamine as the initiator. The properties of those polymers were investigated by Raman spectroscopy, while the morphology was examined using scanning electron microscopy. In particular, it was found that, although the polymerization of 2-aminopyridine could be easily started by oxidative chemical polymerization, the formation of nanostructured polymers was dependent on whether the initiator was used.
Immunogenicity and safety of an acellular pertussis, diphtheria, tetanus, inactivated poliovirus, Hib-conjugate combined vaccine (Pentaxim) and monovalent hepatitis B vaccine at 6, 10 and 14 weeks of age in infants in South Africa.

OBJECTIVE To assess the immunogenicity and safety data for a pentavalent combination vaccine containing acellular pertussis, inactivated poliovirus, and Haemophilus influenzae (Hib) polysaccharide-conjugate antigens. METHODS A DTaP-IPV//PRP T vaccine (Pentaxim) was given at 6, 10 and 14 weeks of age to 212 infants in South Africa. Monovalent hepatitis B vaccine was given concomitantly. Immunogenicity was assessed using seroprotection and seroconversion rates; safety was assessed by monitoring for solicited injection site and systemic adverse events, and follow-up monitoring for unsolicited adverse events and serious adverse events. RESULTS Immunogenicity was high for each vaccine antigen, and similar to a reference study done in France using a similar (2, 3 and 4 months of age) administration schedule. After the third dose, 94.6% of participants had anti-PRP > or = 0.15 microg/ml. The anti-PRP geometric mean antibody titre (GMT) was 2.0 microg/ml. The seroprotection rates for diphtheria and tetanus (> or = 0.01 IU/ml), poliovirus types 1, 2 and 3 (> or = 8 1/dil U) and hepatitis B were all 100%. Anti-polio GMTs were very high, 1 453, 1 699 and 2 398 (1/dil U) for types 1, 2 and 3, respectively. The seroconversion/vaccine response rates to pertussis antigens (4-fold increase in antibody concentration) were 97.5% for PT and 83.9% for FHA. CONCLUSIONS The DTaP-IPV//PRP T vaccine was highly immunogenic at 6, 10 and 14 weeks of age in infants in South Africa, was compatible with the monovalent hepatitis B vaccine, and was well tolerated.
Identification of Vibrio anguillarum Outer Membrane Vesicles Related to Immunostimulation in the Japanese Flounder, Paralichthys olivaceus

We identified outer membrane vesicle (OMV) production in Vibrio anguillarum O1, a major fish pathogen that causes vibriosis, and characterized the OMVs. They were produced during normal growth, and were appeared as spherical vesicle fractions. The protein profile of the OMVs was similar to that of the outer membrane proteins, and the 38-kDa major protein band of OMV was identified as OmpU. The OMVs had enzyme activity of metalloprotease, hemolysin, and phospholipase, and stimulated the production of proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6 when injected into the flounder.
A Major Advance in Elucidating the Biology/Pathobiology of Chlamydia trachomatis

Theseveralchlamydialspecieshavebeenrecognizedfordecades as bacterial pathogens of major importance to humans and domestic animals. Chlamydia trachomatis was first identified as the etiologic agent in blinding trachoma in Africa and the Middle East and later was demonstrated to be a genital pathogen of widespreadoccurrenceinbothdevelopedandunderdevelopedareasof theworld.Indeed,thisorganismisnowconsideredtobethemost common sexually transmitted bacterial pathogen in the United States, with more than a million new such infections reported annually to the Centers for Disease Control and Prevention (1). Significantly, primary genital infection with this organism often engendersseveresequelae,includingfertilityproblemsinwomen, arthritis in both genders, and others (2, 3). Current data indicate that these sequelae involve organisms in an unusual state designated persistence, in which metabolic and genetic activity are modified to yield a largely antimicrobial-refractory organism (3, 4).
Transmural Ventricular Distribution of Coronary Blood Flow during Coronary β2‐Adrenergic Receptor Activation in Dogs

Coronary vessels have β2-adrenergic receptors, but their role in distributing flow across the ventricular wall independently of cardiac metabolic and mechanical factors that influence coronary resistance is unknown. We studied the working heart of open-chest dogs and measured blood flow with radioactive microspheres in four layers across the left ventricular wall before and during β2-receptor activation by intracoronary infusions of salbutamol or isoproterenol. Myocardial β1, receptors were blocked with practolol, and aortic pressure, heart rate, and myocardial oxygen consumption were prevented from changing significantly. β2 activation produced a progressively greater increase in flow from the subendocardium to the subepicardium with a decrease in the subendocardial:subepicardial (I:O) ratio from 1.40 to 1.00. Coronary resistance decreased progressively from the subendocardium to the subepicardium. To assess the influence on the above results of the transmural redistribution of flow that normally is developed by myocardial contraction in the working heart, similar experiments were conducted in the empty beating heart. A two-step vasodilation with salbutamol decreased the I:O flow ratio from 1.76 to 1.40 and then to 1.27. Coronary resistance decreased from the subendocardium to the subepicardium during both steps of vasodilation. For comparable increments of flow, the decrease in the I:O flow ratio in the empty beating heart was smaller than that in the working heart. These results indicate that the activation of coronary β2 receptors redistributes flow toward the subepicardium by producing a larger vasodilation in this region independently of the metabolic and mechanical influences of heart contraction on coronary resistance. This effect is enhanced in the intact heart by the transmural gradient of resistance to flow produced by cardiac contraction. Circ Res 46: 29-36, 1980
Rational design of CCR2 antagonists: a survey of computational studies

Importance of the field: CC-chemokine receptor 2 (CCR2) belongs to the GPCR superfamily and is the primary receptor for monocyte chemoattractant protein-1 (MCP-1), also known as chemokine ligand CCL2. Studies indicate the possible involvement of MCP-1 and CCR2 in various disease conditions, such as rheumatic arthritis, multiple sclerosis, vascular diseases, obesity and diabetes, via the inflammatory pathway. MCP-1 and CCR2 knockout mice under a broad range of stimuli exhibit deficient monocyte recruitment suggesting its potential role in inflammation. Overall, there is evidence that an impairment of monocyte trafficking in inflammation models occurs when there is a loss of MCP-1 effector function. This makes its receptor, CCR2, an attractive target for pharmaceutical research. Several small molecular CCR2 antagonists have been developed, particularly in the industry. Areas covered in this review: In this article, we have summarized the in silico work carried out in the area of CCR2 and reviewed mainly the computer aided drug design (CADD) studies reported on quantitative structure–activity relationship, homology modeling, molecular docking and virtual screening. What the reader will gain: A survey of computational studies for the rational design and development of CCR2 antagonists. Take home message: CADD tools can be used to rationalize the identification of the potential leads and these techniques can be effectively applied in the rapid searching of novel and potent CCR2 antagonists.