Miracle-dz/UniFAR-FTUs huggingface dataset:
Isolation of viable Plasmodium falciparum merozoites to define erythrocyte invasion events and advance vaccine and drug development

During blood-stage infection by Plasmodium falciparum, merozoites invade RBCs. Currently there is limited knowledge of cellular and molecular invasion events, and no established assays are available to readily measure and quantify invasion-inhibitory antibodies or compounds for vaccine and drug studies. We report the isolation of viable merozoites that retain their invasive capacity, at high purity and yield, purified by filtration of highly synchronous populations of schizonts. We show that the half-life of merozoite invasive capacity after rupture is 5 min at 37 °C, and 15 min at room temperature. Studying the kinetics of invasion revealed that 80% of invasion events occur within 10 min of mixing merozoites and RBCs. Invasion efficiency was maximum at low merozoite-to-RBC ratios and occurred efficiently in the absence of serum and with high concentrations of dialyzed nonimmune serum. We developed and optimized an invasion assay by using purified merozoites that enabled invasion-inhibitory activity of antibodies and compounds to be measured separately from other mechanisms of growth inhibition; the assay was more sensitive for detecting inhibitory activity than established growth-inhibition assays. Furthermore, with the use of purified merozoites it was possible to capture and fix merozoites at different stages of invasion for visualization by immunofluorescence microscopy and EM. We thereby demonstrate that processing of the major merozoite antigen merozoite surface protein-1 occurs at the time of RBC invasion. These findings have important implications for defining invasion events and molecular interactions, understanding immune interactions, and identifying and evaluating inhibitors to advance vaccine and drug development.
Redundancy Localization for the Conversationalization of Unstructured Responses

Conversational agents offer users a natural-language interface to accomplish tasks, entertain themselves, or access information. Informational dialogue is particularly challenging in that the agent has to hold a conversation on an open topic, and to achieve a reasonable coverage it generally needs to digest and present unstructured information from textual sources. Making responses based on such sources sound natural and fit appropriately into the conversation context is a topic of ongoing research, one of the key issues of which is preventing the agent’s responses from sounding repetitive. Targeting this issue, we propose a new task, known as redundancy localization, which aims to pinpoint semantic overlap between text passages. To help address it systematically, we formalize the task, prepare a public dataset with fine-grained redundancy labels, and propose a model utilizing a weak training signal defined over the results of a passage-retrieval system on web texts. The proposed model demonstrates superior performance compared to a state-of-the-art entailment model and yields encouraging results when applied to a real-world dialogue.
Presas de mampostería en España

Espana es uno de los paises con mayor tradicion en la construccion de presas de embalse, especialmente en el caso de las presas de fabrica. Las primeras presas de mamposteria fueron construidas por los romanos en el inicio de nuestra era, prolongandose el empleo de este material hasta el segundo tercio del siglo XX. Las presas de mamposteria espanolas conforman un patrimonio hidraulico unico en el mundo, no solo por el numero de presas que lo integran, sino tambien por su reparto a lo largo del tiempo, las dimensiones de las obras, su variedad tipologica, la evolucion de las fabricas petreas empleadas, y su pervivencia hasta fechas tardias del siglo XX. El objetivo de esta tesis es el estudio sistematico y pormenorizado de todas las presas espanolas de mamposteria, articulando el trabajo por periodos homogeneos en la construccion de presas y estructurandolo alrededor de cuatro cuestiones fundamentales: - El diseno de la presa - Los materiales empleados en la construccion - Los organos de alivio - Los metodos y medios constructivos El metodo seguido para estudiar y analizar cada una de estas cuatro cuestiones en los diferentes periodos ha permitido elaborar con una estructura similar las conclusiones parciales para cada uno de los periodos, asi como el establecimiento de las conclusiones generales del trabajo. El conocimiento de la evolucion de las presas de mamposteria resulta de gran importancia en la actualidad, ya que la mayor parte de estas presas continuan en explotacion y requieren de continuos trabajos de conservacion, reparacion y adecuacion.
Adaptive Reduced Feedback Links for Distributed Power Allocation in Multicell MISO-OFDMA Networks

For multi-antenna Orthogonal Frequency-Division Multiple Access (OFDMA) based multicell networks, the channel state information (CSI) of all users is required to share among base stations in order to perform distributed power allocation. However, the amount of feedback increases with the number of users, base stations, subcarriers and antennas. Therefore, it is important to perform a selection at the user side to reduce the feedback load and the complexity of resource allocation. In this letter, we propose adaptive reduced feedback links by choosing the users based on their approximate signal to interference noise ratio (SINR) and their locations in the cell to satisfy users' rate constraints. We illustrate the performance results of reduced feedback links by employing distributed resource allocation with link adaptation.
Elevation of mitochondrial transmembrane potential and reactive oxygen intermediate levels are early events and occur independently from activation of caspases in Fas signaling.

Stimulation of the CD95/Fas/Apo-1 receptor leads to apoptosis through activation of the caspase family of cysteine proteases and disruption of the mitochondrial transmembrane potential (Deltapsim). We show that, in Jurkat human T cells and peripheral blood lymphocytes, Fas-induced apoptosis is preceded by 1) an increase in reactive oxygen intermediates (ROI) and 2) an elevation of Deltapsim. These events are followed by externalization of phosphatidylserine (PS), disruption of Deltapsim, and cell death. The caspase inhibitor peptides, DEVD-CHO, Z-VAD.fmk, and Boc-Asp.fmk, blocked Fas-induced PS externalization, disruption of Deltapsim, and cell death, suggesting that these events are sequelae of caspase activation. By contrast, in the presence of caspase inhibitors, ROI levels and Deltapsim of Fas-stimulated cells remained elevated. Because ROI levels and Deltapsim are regulated by the supply of reducing equivalents from the pentose phosphate pathway (PPP), we studied the impact of transaldolase (TAL), a key enzyme of the PPP, on Fas signaling. Overexpression of TAL accelerated Fas-induced mitochondrial ROI production, Deltapsim elevation, activation of caspase-8 and caspase-3, proteolysis of poly(A)DP-ribose polymerase, and PS externalization. Additionally, suppression of TAL diminished these activities. Therefore, by controlling the balance between mitochondrial ROI production and metabolic supply of reducing equivalents through the PPP, TAL regulates susceptibility to Fas-induced apoptosis. Early increases in ROI levels and Deltapsim as well as the dominant effect of TAL expression on activation of caspase-8/Fas-associated death domain-like IL-1beta-converting enzyme, the most upstream member of the caspase cascade, suggest a pivotal role for redox signaling at the initiation of Fas-mediated apoptosis.
Glomerular filtration rate estimation: performance of serum cystatin C-based prediction equations

Abstract Serum creatinine measurement is a mainstay in the routine laboratory evaluation of renal function, despite of having several disadvantages. Cystatin C, on the other hand, suffers less influence of gender and muscle mass and has been proposed as a more sensitive marker for glomerular filtration rate. However, serum endogenous markers should not be used alone to assess glomerular filtration rate. Creatinine-based equations such as the modification of diet in renal disease (MDRD) and Cockcroft-Gault are widely used despite their limitations. A large number of cystatin C-based prediction equations were developed in recent years, in diverse populations, with different laboratory assays and methods. Several studies demonstrated that cystatin C-based equations are reliable markers of glomerular filtration rate and can be used for diagnosis, evaluation and follow-up of kidney disease. They are simpler than creatinine-based equations and have at least the same accuracy and precision for glomerular filtration rate estimation. In conclusion, diabetes mellitus, cystic fibrosis, kidney transplantation, HIV-infection, and cirrhosis are clinical situations where cystatin C-based equations can be useful. Extremes of age such as childhood and advanced age have also been evaluated with favorable results.
Association between Food for Life, a Whole Setting Healthy and Sustainable Food Programme, and Primary School Children’s Consumption of Fruit and Vegetables: A Cross-Sectional Study in England

The promotion of dietary health is a public health priority in England and in other countries. Research shows that the majority of children do not consume the recommended amount of fruit and vegetables (F&V). There has been relatively little research on the impact of programmes, such as Food for Life, that (a) integrate action on nutrition and food sustainability issues, and (b) are delivered as commissions in a local authority area. The study sought to assess pupil F&V in schools engaged with the Food for Life (FFL) programme. The design was a cross-sectional study comparing pupils in FFL engaged (n = 24) and non-engaged (n = 23) schools. A total of 2411 pupils aged 8–10 completed a validated self-report questionnaire. After adjusting for confounders, pupils in schools engaged with FFL consumed significantly more servings of F&V compared to pupils in comparison schools (M = 2.03/1.54, p < 0.001). Pupils in FFL schools were twice as likely to eat five or more portions of F&V per day (Odds Ratio = 2.07, p < 0.001, Confidence Interval = 1.54, 2.77). Total F&V consumption was significantly higher (p < 0.05) amongst pupils in schools with a higher level FFL award. Whilst limitations include possible residual confounding, the study suggests primary school engagement with the FFL programme may be an effective way of improving children’s dietary health.
Writing to the unknown: bloggers and the presence of backpackers

Purpose – In this paper, the aim is to study virtual presence in travel blogs. The paper seeks to argue that though some conceptualization of virtual presence has been made in the literature, this only took account of one's presence among a community of “known” others. Increasingly, however, in blogging and other online communities, users do not necessarily know the “others” but they nevertheless interact with them and develop friendships and lasting relationships. This stresses the multidimensional character of the study; one which sees presence as the core topic by considering different perspectives to it.Design/methodology/approach – An exploratory case study is conducted with a backpackers' blogging site, its members and audience; a setting that was considered suitable for finding answers to the research questions. The data are analysed qualitatively following a thematic analysis approach.Findings – It was found that the invisible and unknown audience has an important role to play in backpackers' pres.
Characterization of field and vaccine infectious bursal disease viruses from Nigeria revealing possible virulence and regional markers in the VP2 minor hydrophilic peaks

Outbreaks of infectious bursal disease in vaccinated chicken flocks are frequent in Nigeria. For the control of infectious bursal disease, live vaccines based on foreign infectious bursal disease virus (IBDV) strains are used. The present study investigated the phylogenetic relationship between field and vaccine IBDV strains from northwestern Nigeria. Thirty field IBDV strains and three commercial vaccines strains were characterized through sequencing the VP2 hypervariable region. In addition, the complete genome segment A coding region for two vaccines and two field strains was sequenced. The deduced amino acid sequences (position 212 to 331) of IBDV strains from Nigeria and other regions of the world were aligned and possible regional and virulence markers were identified associated with VP2 minor hydrophilic peaks. Reversion to virulence of a vaccine strain with a Q to L mutation at position 253 was observed. Phylogenetic analyses revealed a unique cluster of northwest Nigerian field IBDV strains alone or related to imported characterized classical and very virulent IBDV vaccines. The results suggest that when IBDV strains spread from their region of origin to a different region they mutate alongside indigenous field strains but may retain their identity on the VP2 region.
Interactions between SH2 domains and tyrosine-phosphorylated platelet-derived growth factor beta-receptor sequences: analysis of kinetic parameters by a novel biosensor-based approach

The interaction between SH2 domains and phosphotyrosine-containing sequences was examined by real-time measurements of kinetic parameters. The SH2 domains of the p85 subunit of the phosphatidylinositol 3-kinase as well as of other signaling molecules were expressed in bacteria as glutathione S-transferase fusion proteins. Phosphotyrosine-containing peptides, corresponding to two autophosphorylation sites on the human platelet-derived growth factor beta-receptor that are responsible for phosphatidylinositol 3-kinase binding, were synthesized and used as capturing molecules, immobilized on a biosensor surface. The association and dissociation rate constants for binding to both sites were determined for intact p85 and the recombinant SH2 domains. High association rates were found to be coupled to very fast dissociation rates for all interactions studied. A binding specificity was observed for the two SH2 domains of p85, with the N-terminal SH2 binding with high affinity to the Tyr-751 site but not to the Tyr-740 site, and the C-terminal SH2 interacting strongly with both sites. This approach should be generally applicable to the study of the specificity inherent in the assembly of signaling complexes by activated protein-tyrosine kinase receptors.
Neurological, neuropsychological, and psychosocial outcome following treatment of unruptured intracranial aneurysms: A review and commentary

Thirty studies published between 1977 and 2001 that focus on outcome following unruptured intracranial aneurysm (UIA) treatment are reviewed. Although findings from these studies suggest outcome from UIA treatment is reasonably good (between 5% and 25% morbidity and between 0–7% mortality), many of the complex issues associated with the treatment of UIAs remain controversial. Most of the studies reviewed address outcome in terms of mortality and neurological morbidity. Very few studies exist which include measures of outcome such as cognitive status, psychosocial functioning and quality of life. Given that patients facing treatment tend to be healthy middle-aged adults with many years of active working and social life ahead of them, it is important to take into account the long-term consequences of either harboring an UIA, or having it treated. The small number of studies that include cognitive, psychosocial and quality of life outcomes are reviewed in some detail and suggestions made for improving future UIA outcome research. (JINS, 2004, 10, 114–134.)
Topology, geometry and quantum field theory : proceedings of the 2002 Oxford Symposium in the honour of the 60th birthday of Graeme Segal

Part I. Contributions: 1. A variant of K-theory Michael Atiyah and Michael Hopkins 2. Two-vector bundles and forms of elliptic cohomology Nils Baas, Bjorn Dundas and John Rognes 3. Geometric realisation of the Segal-Sugawara construction David Ben-Zvi and Edward Frenkel 4. Differential isomorphism and equivalence of algebraic varieties Yuri Berest and George Wilson 5. A polarized view of string topology Ralph Cohen and Veronique Godin 6. Random matrices and Calabi-Yau geometry Robbert Dijkgraaf 7. A survey of the topological properties of symplectomorphism groups Dusa McDuff 8. K-theory from a physical perspective Gregory Moore 9. Heisenberg groups and algebraic topology Jack Morava 10. What is an elliptic object? Stephan Stolz and Peter Teichner 11. Open and closed string field theory interpreted in classical algebraic topology Dennis Sullivan 12. K-theory of the moduli of principal bundles on a surface and deformations of the Verlinde algebra Constantin Teleman 13. Cohomology of the stable mapping class group Michael S. Weiss 14. Conformal field theory in four and six dimensions Edward Witten Part II. The Definition of Conformal Field Theory by Graeme Segal: 15. Definition of a conformal field theory Graeme Segal.
Neural Networks for Collective Translational Invariant Object Recognition

A novel method using neural networks for translational invariant object recognition is described in this paper. The objective is to enable the recognition of objects in any shifted position when the objects are presented to the network in only one standard location during the training procedure. With the presence of multiple or overlapped objects in the scene, translational invariant object recognition is a very difficult task. Noise corruption of the image creates another difficulty. In this paper, a novel approach is proposed to tackle this problem, using neural networks with the consideration of multiple objects and the presence of noise. This method utilizes the secondary responses activated by the backpropagation network. A confirmative network is used to obtain the object identification and location, based on these secondary responses. Experimental results were used to demonstrate the ability of this approach.
Repair of Gaps in Retroviral DNA Integration Intermediates

ABSTRACT Diverse mobile DNA elements are believed to pirate host cell enzymes to complete DNA transfer. Prominent examples are provided by retroviral cDNA integration and transposon insertion. These reactions initially involve the attachment of each element 3′ DNA end to staggered sites in the host DNA by element-encoded integrase or transposase enzymes. Unfolding of such intermediates yields DNA gaps at each junction. It has been widely assumed that host DNA repair enzymes complete attachment of the remaining DNA ends, but the enzymes involved have not been identified for any system. We have synthesized DNA substrates containing the expected gap and 5′ two-base flap structure present in retroviral integration intermediates and tested candidate enzymes for the ability to support repair in vitro. We find three required activities, two of which can be satisfied by multiple enzymes. These are a polymerase (polymerase beta, polymerase delta and its cofactor PCNA, or reverse transcriptase), a nuclease (flap endonuclease), and a ligase (ligase I, III, or IV and its cofactor XRCC4). A proposed pathway involving retroviral integrase and reverse transcriptase did not carry out repair under the conditions tested. In addition, prebinding of integrase protein to gapped DNA inhibited repair reactions, indicating that gap repair in vivo may require active disassembly of the integrase complex.
Does Illness Perception Predict Posttraumatic Stress Disorder in Patients with Myocardial Infarction?

INTRODUCTION Myocardial infarction (MI) as a life-threatening event, carrying high risk of recurrence and chronic disabling complications, increases the risk of developing acute stress disorder (ASD), posttraumatic stress disorder (PTSD), or both. The aim of this study was to investigate the relationship between illness perceptions and having ASD, PTSD, or both in patients after MI. METHOD Seventy-six patients diagnosed with acute MI were enrolled into our prospective study. We evaluated patients during the first week and six months after MI. Patients were assessed by using the Clinician Administered PTSD Scale (CAPS), the Hamilton Depression Rating Scale (HDRS), the Hamilton Anxiety Rating Scale (HARS), the Brief Illness Perception Questionnaire (BIPQ), and a semi-structured interview for socio-demographic characteristics during both the first and second evaluations. RESULTS Acute stress disorder (ASD) developed in 9.2% of patients and PTSD developed in 11.9% of patients with MI. Illness perception factors of 'consequences, identity and concern' predicted the occurrence of both ASD and PTSD, whereas 'emotion' predicted only PTSD. CONCLUSION The factors of illness perceptions predicted the induction of ASD and PTSD in patients who had acute MI.
Development of High Signal Intensity within the Globus Pallidus and Dentate Nucleus following Multiple Administrations of Gadobenate Dimeglumine

BACKGROUND AND PURPOSE: Previous studies have evaluated various gadolinium based contrast agents and their association with gadolinium retention, however, there is a discrepancy in the literature concerning the linear agent gadobenate dimeglumine. Our aim was to determine whether an association exists between the administration of gadobenate dimeglumine and the development of intrinsic T1-weighted signal in the dentate nucleus and globus pallidus. MATERIALS AND METHODS: In this single-center, retrospective study, the signal intensity of the globus pallidus, dentate nucleus, thalamus, and middle cerebellar peduncle was measured on unenhanced T1-weighted images in 29 adult patients who had undergone multiple contrast MRIs using exclusively gadobenate dimeglumine (mean, 10.1 ± 3.23 doses; range, 6–18 doses). Two neuroradiologists, blinded to the number of prior gadolinium-based contrast agent administrations, separately placed ROIs within the globi pallidi, thalami, dentate nuclei, and middle cerebellar peduncles on the last MR imaging examinations. The correlations between the globus pallidus:thalamus and the dentate nucleus:middle cerebellar peduncle signal intensity ratios with the number of gadolinium-based contrast agent administrations and cumulative dose were tested with either 1-tailed Pearson or Spearman correlations. A priori, P < . 05 was considered statistically significant. RESULTS: Both the globus pallidus:thalamus and dentate nucleus:middle cerebellar peduncle ratios showed significant correlation with the number of gadolinium-based contrast agent administrations (r = 0.39, P = .017, and r = 0.58, P = .001, respectively). Additionally, the globus pallidus:thalamus and dentate nucleus:middle cerebellar peduncle ratios showed significant correlation with the cumulative dose of gadobenate dimeglumine (r = 0.48, P = .004, and r = 0.43, P = .009, respectively). Dentate nucleus hyperintensity was qualitatively present on the last MR imaging in 79.3%–86.2% of patients and in all patients who had received >10 doses. CONCLUSIONS: At high cumulative doses (commonly experienced by patients, for example, with neoplastic disease), gadobenate dimeglumine is associated with an increase in the globus pallidus:thalamus and dentate nucleus:middle cerebellar peduncles signal intensity ratios.