Miracle-dz/UniFAR-FTUs huggingface dataset:
The Mechanism of Dephosphorylation of Extracellular Signal-regulated Kinase 2 by Mitogen-activated Protein Kinase Phosphatase 3*

The mitogen-activated protein (MAP) kinase phosphatase-3 (MKP3) is a dual specificity phosphatase that specifically inactivates one subfamily of MAP kinases, the extracellular signal-regulated kinases (ERKs). Inactivation of MAP kinases occurs by dephosphorylation of Thr(P) and Tyr(P) in the TXY kinase activation motif. To gain insight into the mechanism of ERK2 inactivation by MKP3, we have carried out an analysis of the MKP3-catalyzed dephosphorylation of the phosphorylated ERK2. We find that ERK2/pTpY dephosphorylation by MKP3 involves an ordered, distributive mechanism in which MKP3 binds the bisphosphorylated ERK2/pTpY, dephosphorylates Tyr(P) first, dissociates and releases the monophosphorylated ERK2/pT, which is then subjected to dephosphorylation by a second MKP3, yielding the fully dephosphorylated ERK2. The bisphosphorylated ERK2 is a highly specific substrate for MKP3 with a k cat/K m of 3.8 × 106 m −1s−1, which is more than 6 orders of magnitude higher than that for small molecule aryl phosphates and an ERK2-derived phosphopeptide encompassing the pTEpY motif. This strikingly high substrate specificity displayed by MKP3 may result from a combination of high affinity binding interactions between the N-terminal domain of MKP3 and ERK2 and specific ERK2-induced allosteric activation of the MKP3 C-terminal phosphatase domain.
Efficient Digital Background Calibration of Time-Interleaved Pipeline Analog-to-Digital Converters

A novel technique for the digital background calibration of time-interleaved analog-to-digital converters is proposed. The technique corrects at the same time for both errors due to gain, offset and timing mismatches among the time-interleaved channels and errors due to nonlinearities in the channels, for instance due to capacitor mismatches in switched capacitor implementations. This feature, together with the use of the recursive least mean squares algorithm, makes the technique particularly fast (12 bits of accuracy can be achieved after about 4000 samples for a two-channel converter). The proposed calibration technique employs wideband differentiators, thus enabling digital background calibration of timing skews even with wideband input signals. Besides, undersampled differentiator filters are proposed, and it is shown that the technique is capable of calibrating undersampling converters by estimating the derivative of wideband input signals even outside the first Nyquist band.
Assessment of aqueous humor dynamics in the mouse by a novel method of constant-flow infusion.

PURPOSE To characterize a technique that concurrently assesses all aqueous humor hydrodynamic parameters in mouse eyes. METHODS Mouse outflow facility (C) was determined by multiple flow-rate infusion and episcleral venous pressure (Pe) measured by manometry. The animals were then euthanatized, eliminating aqueous formation rate (Fin) and Pe. C was determined again (C(dead)) while uveoscleral outflow (Fu(dead)) and Fin were deduced. To assess whether Fu(dead) would remain the same as Fu(live), the animals were perfused with FITC-dextran and Fu determined. The effects of IOP-lowering drugs on the parameters of aqueous hydrodynamics were also evaluated. RESULTS Under the conditions tested, Fu(live) (0.012 ± 0.003 μL/min) was not different from Fu(dead) (0.015 ± 0.003 μL/min; P = 0.472). In anesthetized mice, IOP = 11.4 ± 0.2 mm Hg (mean ± SEM, n = 8), C = 0.018 ± 0.0006 μL/min/mm Hg, Pe = 5.4 ± 0.2 mm Hg, Fin = 0.14 ± 0.0007 μL/min, and Fu = 0.029 ± 0.005 μL/min. C(dead) was not different from C (P = 0.317). Latanoprost reduced IOP by increasing C by 0.009 ± 0.0003 μL/min/mm Hg (P < 0.001), without affecting Fin or Fu. Betaxolol reduced Fin by 0.075 ± 0.021 μL/min (P = 0.009). Brimonidine increased C by 0.005 ± 0.0005 μL/min/mm Hg (P < 0.001) and Fu by 0.013 ± 0.003 μL/min (P = 0.007). CONCLUSIONS In this study, a unique technique was developed to concurrently assess IOP, C, Pe, Fin, and Fu in the mouse eye. This experimental approach should be useful to evaluate effects of pharmacologic agents or genetic manipulations on aqueous humor dynamics in mice and other animal models.
Mapping Distance Graph Kernels using Bipartite Matching

The objective of graph classification is to classify graphs o f imilar structures into the same class. This problem is of key importance in areas such as cheminformatics and bioinformatics. Support Vector Machines can efficiently classify graphs if graph kernels are used instea d of feature vectors. In this paper, we propose two novel and efficient graph kernels called Mapping Distance Ke rnel with Stars (MDKS) and Mapping Distance Kernel with Vectors (MDKV). MDKS approximately measures th e graph edit distance using star structures of height one. The method runs in O(υ3), whereυ is the maximum number of vertices in the graphs. However, when the height of the star structures is increased to avoid s tructural information loss, this graph kernel is no longer efficient. Hence, MDKV represents star structures of height greater than one as vectors and sums their Euclidean distances. It runs in O(h(υ3 + |Σ|υ2)), whereΣ is a set of vertex labels and graphs are iteratively relabeledh times. We verify the computational efficiency of the propose d graph kernels on artificially generated datasets. Further, results on three real-world datase ts show that the classification accuracy of the proposed graph kernels is higher than three conventional graph kerne l methods.
Design of microstrip ultrabroadband multisection quadrature hybrid

This paper proposes a three- and a five-section microstrip 3-dB quadrature hybrid. Two types of enhanced coupling structures, both with a rectangular ground-plane aperture and two inserted signal strips in the aperture, are used in the quadrature hybrid not only to achieve tight coupling but also to minimize the junction effect between coupling sections. The designed circuits have a fully planar structure. They were fabricated on a single-layer substrate by the standard printed circuit board (PCB) manufacturing process.
The Return to Independent Invention: Evidence of Risk Seeking, Extreme Optimism or Skewness-Loving

Examining a sample of 1,091 inventions I investigate the magnitude and distribution of the pretax internal rate of return (IRR) to inventive activity. The average IRR on a portfolio investment in these inventions is 11.4%. This is higher than the risk-free rate but lower than the long-run return on high-risk securities and the long-run return on early-stage venture capital funds. The portfolio IRR is significantly higher, for some ex ante identifiable classes of inventions. The distribution of return is skew: only between 7-9% reach the market. Of the 75 inventions that did, six realised returns above 1400%, 60% obtained negative returns and the median was negative.
Accuracy of the clinical evaluation for frontotemporal dementia.

BACKGROUND Without a definitive clinical test, the early diagnosis of frontotemporal dementia (FTD) can be difficult. OBJECTIVE To evaluate the accuracy of the clinical evaluation for FTD. DESIGN Retrospective assessment of consensus criteria for FTD, neuropsychological measures, magnetic resonance images, and single-photon emission computed tomography/positron emission tomography (SPECT/PET) scans at baseline compared with a standard of subsequent clinical diagnosis after follow-up and reevaluation to year 2. SETTING University hospital. PATIENTS A total of 134 patients referred for clinical evaluation of suspected FTD. These patients had 1 or more core or supportive features of FTD in the absence of another etiology on initial assessment. MAIN OUTCOME MEASURES Sensitivities, specificities, and predictive values of consensus criteria for FTD, magnetic resonance images, and SPECT/PET scans at initial assessment. RESULTS The sensitivities and specificities for the diagnosis of FTD were 36.5% and 100.0% for consensus criteria, 63.5% and 70.4% for magnetic resonance images, and 90.5% and 74.6% for SPECT/PET scans, respectively. With a previous prevalence of nearly 50% for FTD, the positive predictive value was greatest for consensus criteria (100.0%), and the negative predictive value was greatest for SPECT/PET (89.8%). The initial neuropsychological results did not distinguish FTD, but the pattern of progression (worse naming and executive functions and preserved constructional ability) helped establish the diagnosis at year 2. CONCLUSIONS Consensus criteria for FTD and neuropsychological measures lacked sensitivity for FTD; however, neuroimaging, particularly functional brain studies, greatly increased the sensitivity of detecting FTD. The clinical diagnosis of FTD needs to combine neuropsychiatric features with SPECT or PET findings while following the changes on neuropsychological tests.
Five Dimensions of Wellness and Predictors of Cognitive Health Protection in Community-Dwelling Older Adults

Wellness is associated with cognitive health protection; however, findings are limited because they only examine variable(s) within one dimension of wellness. This research examined the association between multiple dimensions of wellness and cognition among aging adults. The sample included 5,605 male and female community-dwelling adults 60 years and older. Four dimensions of wellness demonstrated a statistically significant higher mean difference in cognitively healthy older adults compared to cognitively impaired older adults, F(4, 5,595) = 47.57, p < . 001. Emotional wellness demonstrated the strongest association with cognitive health, followed by physical and spiritual wellness, F(5, 5,372) = 50.35, p < . 001. Future research is needed to examine the cognitive protective benefits of wellness using longitudinal, prospective designs that control for the potential temporal relationship between wellness and cognition.
Evaluation of gamma radiation-induced DNA damage in Aedes aegypti using the comet assay

The study was undertaken to evaluate gamma radiation-induced DNA damage in Aedes aegypti. The comet assay was employed to demonstrate the extent of DNA damage produced in adult male A. aegypti exposed to seven different doses of gamma radiation, ranging from 1 Gy to 50 Gy. DNA damage was measured as the percentage of comet tail DNA. A significant linear increase in DNA damage was observed in all samples; the extent of damage being proportional to the dose of gamma radiation the organism received, except in those treated with 1 Gy. The highest amount of DNA damage was noticed at 1 h postirradiation, which decreased gradually with time, that is, at 3, 6 and 12 h postirradiation. This may indicate repair of the damaged DNA and/or loss of heavily damaged cells as the postirradiation time increased. The comet assay serves as a sensitive and rapid technique to detect gamma radiation-induced DNA damage in A. aegypti. This could be used as a potential biomarker for environmental risk assessment.
Transurethral Microwave Thermotherapy for Symptomatic Benign Prostatic Hyperplasia: Short-Term Experience with Prostcare

Purpose: To assess our short-term experience with transurethral microwave thermotherapy (TUMT) for symptomatic benign prostatic hyperplasia (BPH). Patients and Methods: From August 1993 through July 1994, in total 65 patients with symptomatic BPH were enrolled into this study. The patients’ ages ranged from 56 to 95 years with a mean of 70 years. Under local anesthesia with intraurethral instillation of Xylocaine jelly only, all patients received one session of TUMT for up to 60 min with Prostcare equipment. Uroflowmetry was performed and international prostatic symptom score (IPSS) determined before 3 and 6 months after TUMT for assessment of efficacy. All adverse events were recorded and evaluated for clinical relevance. Results: At 3 and 6 months following TUMT, the mean IPSS decreased from 19.7 ± 6.8 (baseline) to 12.8 ± 8.2 (–46%) and to 15.5 ± 9.0 (–21%), respectively; the maximal urine flow rate at 3 and 6 months increased from 9.1 ± 4.8 ml/s (baseline) to 11.0 ± 4.9 ml/s (+21%) and to 10.9 ± 5.6 ml/s (+19%), respectively. During TUMT, burning sensation was the most frequent complaint (38.5%), followed by urethral discomfort (29.2%) and urgency (9.2%). Two patients (3.1%) interrupted TUMT, because of intolerable pain. Following TUMT micturition pain (73.8%) and gross hematuria (45.9%) were the most adverse events. Most of these adverse events disappeared within 2 weeks. One patient suffered from skin erosion over the penoscrotal junction 1 week later. None had retrograde ejaculation; 1 patient complained of erectile dysfunction. Conclusion: Although the efficacy of TUMT with Prostcare became less prominent 6 months after TUMT, TUMT was still a tolerable, safe alternative treatment of BPH, especially in patients who were not suitable for transurethral resection of the prostate or anesthesia.
Phylogeographical structure in mitochondrial DNA of eggplant fruit and shoot borer, Leucinodes orbonalis Guenée (Lepidoptera: Crambidae) in South and Southeast Asia

Abstract Leucinodes orbonalis is the most detrimental South and Southeast Asian insect pest of eggplant. To help reduce the impact of this pest, population genetic diversity and structure of L. orbonalis were examined in eight populations from six countries using mitochondrial cytochrome c oxidase subunit I DNA sequences. No correlation between genetic diversity and geographic distance was detected among populations. Low levels of haplotype and nucleotide diversities were observed in the Philippines population, suggesting recent colonization. No significant gene flow was found among local populations in different countries. The Vietnam population is highly differentiated, indicated by significant pairwise FST values, and may be ascribed to a new subspecies or race. India was confirmed to be the source of genetic variation in L. orbonalis populations. Our study showed that L. orbonalis formed subpopulations for each local region, and the corresponding pest management technology should be developed at the country scale.
Was it worth the trouble? Limited competition and citizen well-being under authoritarian rule

The last part of 20th century saw the collapse of a dramatic number of dictatorships. Rather than democracy, several of these transitions brought regimes where limited political competition coexists with persistently authoritarian practices. The diffusion of this form of authoritarianism in the developing world raises several questions about its broader consequences. Most importantly, does political change short of democratization matter for ordinary citizens? Recent research demonstrates that nominally democratic institutions, even in the absence of people empowerment, can result in better living conditions. The paper adds to this debate by formulating and testing new hypotheses. I compare electoral authoritarianism with democracy and full dictatorship, including specific subtypes of the latter, and focus on both policy outputs and outcomes.
Dampak Implementasi Desentralisasi Fiskal Terhadap Pertumbuhan Ekonomi Dan Stabilitas Harga Di Provinsi Di Indonesia

Abstrak Desentralisasi fiskal dan pelaksanaan otonomi daerah meningkatkan kewenangan pemerintah daerah untuk meningkatkan pendapatan mereka dan melakukan fungsi alokatif dalam menetapkan prioritas pembangunan daerah. Desentralisasi fiskal dan pelaksanaan otonomi daerah diharapkan untuk meningkatkan kesetaraan perkembangan lokal, sesuai dengan motivasi pemerintah daerah untuk mengembangkan daerah mereka berdasarkan potensi khusus mereka. Penelitian ini dimaksudkan untuk menguji pengaruh desentralisasi fiskal terhadap pertumbuhan ekonomi, dan inflasi di provinsi-provinsi di Indonesia. Analisis data dilakukan dengan menggunakan regresi berganda. Hasil penelitian ini menunjukkan bahwa, desentralisasi fiskal memiliki hubungan yang positif tetapi secara statistik tidak berpengaruh signifikan terhadap pertumbuhan ekonomi dan inflasi. Kedua, kerja berpengaruh positif signifikan terhadap pertumbuhan ekonomi. Kata kunci : Desentralisasi fiskal, pertumbuhan ekonomi, inflasi, lapangan kerja. Abstract Fiscal decentralization and implementation of local autonomy enhance authority of local governments to increase their income and to conduct allocative function in setting priority of local development. Fiscal decentralization and implementation of local autonomy are expected to improve equality of local developments, in accordance with the motivation of local governments to develop their region based on their specific potentiality. This study is intended to examine the influence of fiscal decentralization on economic growth, and inflation in the provinces in Indonesia. Data analysis is conducted using multiple regression. The results of this study indicate that, fiscal decentralization has a positive relationship but statistically were not significant effect on economic growth and inflation. Second, employment significant positive effect on economic growth . Keywords: Fiscal decentralization, economic growth, inflation, employment.
Allergenic Components of Indian Jujube (Zizyphus mauritiana) Show IgE Cross-Reactivity with Latex Allergen

Background: ‘Latex-fruit syndrome’ has been well documented. A prevalence of latex allergy among medical workers of 6.8–8.6% had been reported in Taiwan. However, there has been no study to determine the importance and type of fruit hypersensitivity in latex-allergic patients in Taiwan. This study aimed to identify the allergenic components of Indian jujube (Zizyphus mauritiana) and characterize the cross-reactivity of specific IgE antibodies to latex allergen. Methods: Crude extracts were prepared from Indian jujube and from ammoniated natural rubber latex, and six medical workers and one patient with a history of fruit allergy underwent skin testing with routine allergens, latex, Indian jujube and other fruits. Sera from two Indian jujube skin test-positive latex-allergic subjects were used for allergen-specific IgE, immunoblotting, immunoblot inhibition and enzyme-linked immunosorbent assay (ELISA) inhibition studies. Results: Both patients had positive skin test responses and specific IgE assays to Indian jujube and latex extracts. Immunoblotting revealed that IgE from both subjects bound to a 42-kD latex protein and a 42-kD Indian jujube protein. In addition, IgE from one subject bound to a 30-kD Indian jujube protein. Preincubation of atopic sera with Indian jujube or latex extract demonstrated absent and/or marked inhibition of IgE binding. Moreover, anti-Indian jujube protein antibody-based ELISA was able to detect latex extracts. Conclusions: Our results add to findings regarding the ‘latex-fruit syndrome’ described in the literature, and further study of the cross-reacting allergens identified in Indian jujube may help to elucidate the mechanisms underlying this syndrome.
The Mdm2 and p53 genes are conserved in the Arachnids

The p53 protein and its negative regulator the ubiquitin E3 ligase Mdm2 have been shown to be conserved from the Placazoan to man. In common with D.melanogaster and C.elegans, there is a single copy of the p53 gene in T.adhaerens, while in the vertebrates three p53-like genes can be found: p53 , p63 and p73. The Mdm2 gene is not present within the fully sequenced and highly annotated genomes of C.elegans and D.melanogaster. However, it is present in the Placazoan and the presence of multiple distinct p53 genes in the Sea anemone N.vectensis led us to examine the genomes of other phyla for p53 and Mdm2-like genes. We report here the discovery of an Mdm2-like gene and two distinct p53 like genes in the Arachnid Ioxodes scapularis (Northern Deer Tick). The two predicted Deer Tick p53 proteins are much more highly related to the human p53 protein in sequence than are the fruit fly and nematode proteins. One of the Deer tick genes encodes a p53 protein that is initiated within the DNA binding domain of p53 and shows remarkable homology to the newly described N-terminally truncated delta isoforms of human and zebrafish p53.
A 5-year retrospective review of adverse drug reactions and their risk factors in human immunodeficiency virus-infected patients who were receiving intravenous pentamidine therapy for Pneumocystis carinii pneumonia.

The incidence and severity of adverse drug reactions (ADRs) in human immunodeficiency virus-infected persons receiving intravenous pentamidine for Pneumocystis carinii pneumonia during a 5-year period were reviewed retrospectively. Predisposing risk factors for ADRs were identified. ADRs were included if they occurred during or within 1 week following the discontinuation of pentamidine treatment. Nephrotoxicity, dysglycemia, hepatotoxicity, hyperkalemia, and hyperamylasemia accounted for 80% of ADRs (n = 174) that occurred in 76 (71.7%) of 106 patients during 84 treatment courses of pentamidine. A significant relationship between hypoglycemia and nephrotoxicity was observed (P = .002). Four factors were significantly associated with occurrence of an ADR: number of concomitant medications (odds ratio [OR] = 1.36, P = .005), nonwhite ethnicity (OR = 5.00, P = .017), cumulative dosage of pentamidine (OR = 1.03, P = .030), and concurrent use of other nephrotoxic drugs (OR = 2.34, P = .047). Two factors, daily dosage and history of intravenous drug use, approached significance. Knowledge of and avoidance of potential risk factors might allow safer use of pentamidine and reduce the prevalence of ADRs.
A woman lost in the cemetery: A case of time-limited amnesia

A 43-year-old woman one day experienced a dissociative fugue which she could not recall. She was married, nulliparous, with no history of dissociative disorder or other psychiatric disorders. She had been sexually abused during late childhood–early adolescence. She was examined thoroughly from both psychiatric and medical standpoints to exclude organic causes for her condition. Magnetic Resonance Imaging showed only some non-specific abnormalities. On personality tests, a histrionic structure of personality emerged, with obsessive and narcissistic traits accompanied by rigidity and anxiety, dysphoria and high risk for depression; some impairment was found in executive function tests. Final diagnosis was one of dissociative fugue. In fact, organic traits were not sufficient to establish a diagnosis of Transient Global Amnesia.
Screening for depression in the older adult: criterion validity of the 10-item Center for Epidemiological Studies Depression Scale (CES-D)

BACKGROUND The Center for Epidemiological Studies Depression Scale (CES-D) has been widely used in studies of late-life depression. While the CES-D is convenient to use in most settings, it can present problems for elderly respondents who may find the response format confusing, the questions emotionally stressful, and the time to complete burdensome. A briefer 10-item version has been proposed, but there are few data on its properties as a screening instrument. METHODS The 10-item CES-D was administered in 2 studies. In study 1, a stratified sample of middle-aged depressed patients (n = 40) and comparison controls (n = 43) were administered the CES-D to determine an optimal cutoff score. In study 2, the accuracy of the CES-D optimal cutoff score was tested in a sample of adults older than 60 years (n = 68). Major depression diagnoses were derived from the Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders, Revised Third Edition, with consensus diagnoses using Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition. RESULTS Reliability statistics with the 10-item CES-D were found to be comparable to those reported for the original CES-D. Using an optimal cutoff score of 4 in study 1, the sensitivity of the 10-item CES-D was 97%; specificity, 84%; and positive predictive value, 85%. In the study 2 sample of older adults, the sensitivity of the CES-D was 100%; specificity, 93%; and positive predictive value, 38%. CONCLUSION The 10-item CES-D has excellent properties for use as a screening instrument for the identification of major depression in older adults.