Miracle-dz/UniFAR-FTUs huggingface dataset:
ACTH Modulates PTP‐PEST Activity and Promotes Its Interaction With Paxillin

Adrenocorticotropic hormone (ACTH) treatment has been proven to promote paxillin dephosphorylation and increase soluble protein tyrosine phosphatase (PTP) activity in rat adrenal zona fasciculata (ZF). Also, in‐gel PTP assays have shown the activation of a 115‐kDa PTP (PTP115) by ACTH. In this context, the current work presents evidence that PTP115 is PTP‐PEST, a PTP that recognizes paxillin as substrate. PTP115 was partially purified from rat adrenal ZF and PTP‐PEST was detected through Western blot in bioactive samples taken in each purification step. Immunohistochemical and RT‐PCR studies revealed PTP‐PEST expression in rat ZF and Y1 adrenocortical cells. Moreover, a PTP‐PEST siRNA decreased the expression of this phosphatase. PKA phosphorylation of purified PTP115 isolated from non‐ACTH‐treated rats increased KM and VM. Finally, in‐gel PTP assays of immunoprecipitated paxillin from control and ACTH‐treated rats suggested a hormone‐mediated increase in paxillin–PTP115 interaction, while PTP‐PEST and paxillin co‐localize in Y1 cells. Taken together, these data demonstrate PTP‐PEST expression in adrenal ZF and its regulation by ACTH/PKA and also suggest an ACTH‐induced PTP–PEST–paxillin interaction. J. Cell. Biochem. 117: 2170–2181, 2016. © 2016 The Authors. Journal of Cellular Biochemistry Published by Wiley Periodicals, Inc.
DETECTION AND IDENTIFICATION OF Legionella SPECIES FROM GROUNDWATERS

Legionellae are opportunistic bacterial pathogens causing Legionnaires’ disease and Pontiac fever and are ubiquitous in surface waters and in infrastructure to contain or distribute water, including pipes, cooling towers, and whirlpool spas. Infection in community-acquired and nosocomial outbreaks is by exposure to contaminated aerosols. Little is known about the presence of legionellae in groundwater. This study used samples from various locations in the United States and Canada to determine if legionellae could be isolated from water and biofilms derived from groundwaters not known to be under the direct influence of surface water. Of the 114 total samples of water and biofilm tested, 29.1% and 28.2% were positive for Legionella by cultivation and polymerase chain reaction (PCR), respectively. Legionellae were found in both warm and colder groundwaters, with more isolates from samples incubated at 30°C than the 35°C conventional temperature for Legionella isolation. The concentration of Legionella found in the water samples ranged from 102 to 105 CFU/L and up to 1.2 × 102 CFU/cm2 in the biofilm. The species of Legionella identified included both known pathogenic species and species that have not yet been identified as human pathogens. Millions of people in Canada, and around the world, rely on groundwater as their source for drinking. This study shows that legionellae are widespread in groundwater and have the potential to seed derived water supplies and biofilms in public distribution systems. This further widens the known sphere of Legionella colonization and the implications of its presence for public health.
A randomized clinical trial of a wellness intervention for women with multiple sclerosis.

OBJECTIVE To examine the effects of a wellness intervention program for women with multiple sclerosis (MS) on health behaviors and quality of life (QOL). DESIGN Randomized clinical trial. SETTING Community setting in the southwestern United States. PARTICIPANTS Convenience sample of 113 women with physician-confirmed MS (mean age, 45.79y). INTERVENTIONS The 2-phase intervention program included lifestyle-change classes for 8 weeks, then telephone follow-up for 3 months. Participants were followed over an 8-month period. MAIN OUTCOME MEASURES A series of self-report instruments to measure barriers, resources, self-efficacy for health behaviors, health promotion behaviors, and health-related QOL were completed at baseline, 2 months (after the classes), 5 months (after telephone follow-up), and at 8 months. Principal outcomes measures were health-promoting behaviors (scores on the Health Promoting Lifestyle Profile II) and QOL (scores on the Medical Outcomes Study 36-Item Short-Form Health Survey [SF-36] scales). RESULTS Hierarchical linear modeling techniques revealed a statistically significant group by time effect for self-efficacy for health behaviors, health-promoting behaviors, and the mental health and pain scales of the SF-36. CONCLUSION These data provide initial support for the positive effects of wellness interventions to improve health behaviors and selected dimensions of QOL for women with MS.
Slowing down of North Pacific climate variability and its implications for abrupt ecosystem change

Significance Sea surface temperature (SST) variations in the North Pacific have triggered past abrupt changes in fisheries and other ecosystems. We have discovered that over the last century, fluctuations of North Pacific SSTs have become less frequent and longer-lived. This “reddening” behavior can also be seen in the dominant pattern of climate variability in the region, known as the Pacific Decadal Oscillation index. This fundamental change in climate variability has important implications for ecosystems in the region. It implies that over the last century, ecosystems have become prone to undergoing larger climate-triggered abrupt shifts. Hence our discovery of changing climate variability could have contributed to the large magnitude of well-known abrupt changes in North Pacific ecosystems in 1977 and 1989. Marine ecosystems are sensitive to stochastic environmental variability, with higher-amplitude, lower-frequency––i.e., “redder”––variability posing a greater threat of triggering large ecosystem changes. Here we show that fluctuations in the Pacific Decadal Oscillation (PDO) index have slowed down markedly over the observational record (1900–present), as indicated by a robust increase in autocorrelation. This “reddening” of the spectrum of climate variability is also found in regionally averaged North Pacific sea surface temperatures (SSTs), and can be at least partly explained by observed deepening of the ocean mixed layer. The progressive reddening of North Pacific climate variability has important implications for marine ecosystems. Ecosystem variables that respond linearly to climate forcing will have become prone to much larger variations over the observational record, whereas ecosystem variables that respond nonlinearly to climate forcing will have become prone to more frequent “regime shifts. ” Thus, slowing down of North Pacific climate variability can help explain the large magnitude and potentially the quick succession of well-known abrupt changes in North Pacific ecosystems in 1977 and 1989. When looking ahead, despite model limitations in simulating mixed layer depth (MLD) in the North Pacific, global warming is robustly expected to decrease MLD. This could potentially reverse the observed trend of slowing down of North Pacific climate variability and its effects on marine ecosystems.
Local thickness and anisotropy approaches to characterize pore size distribution of three-dimensional porous networks.

Two image-analysis approaches for pore size distribution (PSD) of porous media are proposed. The methods are based on the skeleton representation of a porous object. One approach gives the local thickness of the pore object to represent the pore size corresponding to a lower limit of PSD. The other gives the pore size taking into account the anisotropy of pore object and corresponds to an upper limit of PSD. These two approaches can be incorporated into a computer program without computationally intensive and complex mathematical operations. In this study, these two approaches are applied to a two-dimensional (2D) synthetic image and 3D natural images of tissue scaffolds with various porosities and tortuosities. The scaffolds were prepared by removing the water-soluble poly(ethylene oxide) (PEO) component of the polycaprolactone (PCL)/PEO blend, leaving a porous PCL scaffold. Extracting quantitative PSD information for materials with an interconnected porous network rather than discrete voids (such as tissue scaffolds) is inevitably subjective without a universally accepted definition of "pore size. " Therefore, the proposed lower and upper limits of PSD can come into play when considering mass transfer and scaffold surface area for cell-matrix interaction.
Variability resilient low-power 7T-SRAM design for nano-scaled technologies

High variability in nano-scaled technologies can easily disturb the stability of a carefully designed standard 6T-SRAM cell, causing access failures during a read/write operation. We propose a 7T-SRAM cell to increase the read/write stability under large variations. The proposed design uses a low overhead read/write assist circuitry to increase the noise immunity. Use of an additional transistor and a floating ground allows read disturb free operation. While the write assist circuitry provides a floating ground during a write operation that weakens cell storage by turning off the supply voltage to ground path of the cross-coupled inverter pair. This allows a high speed/low power write operation. Monte Carlo simulations indicate a 200% increase in the read stability and a boost of 124% in write stability compared to a conventional 6T-SRAM design, when subjected to random dopant fluctuations, line edge roughness, and poly-granularity variations. HSPICE simulations of a 45nm 64×32 bit SRAM array designed using standard 6T and proposed 7T SRAM cells indicate a 31% improvement in write speed/write power, read power decreases by 60%, and a 44% reduction in the total average power consumption is achieved with the proposed design.
Treatment with a combination of intra-oral sensory stimulation and electropalatography in a child with severe developmental dyspraxia

This paper describes the use of a combination of intra-oral sensory stimulation and electropalatography (EPG) in the treatment of a case with severe developmental verbal dyspraxia. A multiple-baseline design was used. The treatment duration was 11 months and started when the subject was 5 years old. The efficacy of the treatment was assessed by calculations of percentage of correctly articulated words, percentage of consonants correct, percentage of phonemes correct and percentage of words correct. Intelligibility assessments were conducted by both naïve and expert listeners. The experts also assessed visual deviances in articulatory gestures from video recordings. Qualitative analysis of EPG data was made. The subject's speech was significantly improved by the treatment in all aspects. The results and their generalization to other cases of developmental verbal dyspraxia are discussed.
A New Rabbit Monoclonal Antibody (4B5) for the Immunohistochemical (IHC) Determination of the HER2 Status in Breast Cancer: Comparison With CB11, Fluorescence In Situ Hybridization (FISH), and Interlaboratory Reproducibility

The 2 methodologies in current clinical use to assess HER2 status in breast cancer are: fluorescence in situ hybridization (FISH) (gene amplification) and immunohistochemistry (protein over-expression). A consistent finding has been that 3% to 15% of breast cancers over-express HER2 protein without evidence for gene amplification. Accurate determination of the HER2 status has implications for selecting patients most likely to respond to trastuzumab. We report here our preliminary experience with a new anti-HER2 rabbit monoclonal antibody, 4B5. The evaluation of HER2 status in 2 different cohorts of breast cancer cases (Single Institution (SI) and Multinational (MN)) with a total of 322 breast cancer cases was performed on an automated staining system (Ventana Medical Systems, Inc, Tucson, AZ) and scored by 3 pathologists (0-3+), for comparison with CB11 staining results (PATHWAY) and FISH (PathVysion). Interlaboratory reproducibility of automated staining results and interpretation was determined on a subset of the SI cohort at 3 separate laboratories. Rabbit monoclonal 4B5 demonstrated sharper membrane staining with less cytoplasmic and stromal background staining than CB11. In the SI cohort, the staining results for 4B5 were highly comparable with those obtained for CB11 with an overall concordance of 93.3%. In the multinational cohort, the overall concordance with CB11 was 84.7%. This lower level of concordance was associated with a much higher overall agreement of 4B5 with FISH (89.5%), compared with agreement of CB11 with FISH (81.2%). The difference in the performance of CB11 in the MN cohort versus the SI cohort may be due to differences in tissue fixation and processing in a centralized, high volume laboratory in an academic medical center versus multiple sites in the international community with potentially nonstandardized techniques. The staining results with 4B5 indicate that it has a more robust performance than CB11 because the correlation of 4B5 with FISH was nearly equivalent (88.2% MN; 89.3% SI) in both cohorts. Interlaboratory reproducibility was also excellent (κ 1.0). RMoAb 4B5 provides excellent sensitivity, specificity, and interlaboratory reproducibility for the detection of HER2 status in breast cancer.
Prevention of Blackhole Attack in MANET

An ad hoc network is a collection of mobile nodes that dynamically form a temporary network. It operates without the use of existing infrastructure. One of the principal routing protocols used in ad-hoc networks is AODV (ad-hoc on demand distance vector) protocol. The security of the AODV protocol is compromised by a particular type of attack called 'black hole' attack. In this attack a malicious node advertises itself as having the shortest path to the node whose packets it wants to intercept. To reduce the probability it is proposed to wait and check the replies from all the neighboring nodes to find a safe route. Computer simulation using glomosim shows that our protocol provides better performance than the conventional AODV in the presence of Black holes with minimal additional delay and Overhead.
Trickle or a Flood: Commitments and Disbursement for HIV/AIDS from the Global Fund, PEPFAR, and the World Bank's Multi-Country AIDS Program (MAP)

This paper provides an analytical framework for understanding funders’ disbursement policies and practices while also offering an overview of the total volume of resources being committed and disbursed by each funder. The analysis is focused on the global-level, but does provide brief country case studies to help understand some of the implications of these large inflows of funding for HIV/AIDS at the country-level.
Professional Development Schools and Teacher Educators' Beliefs: Challenges and Change.

The process-product paradigm of teaching and learning has recently been challenged by a more constructivist view that empowers the learner. This democ ratizing of teaching and learning has found expression in teacher education through increasing collaborative efforts between universities and public schools for preservice teacher education Paul DeWitt is with the (Zeichner, 1992;Birrell&Tibbitts,inpress). Univer Lindon Elementary sity/school partnerships promise much toward im School in Pleasant proved teacher education, empowerment and re Grove, Utah, and James newal of classroom teachers, and greater voice for R. Birrell, M. Winston undergraduate students in their own education Egan, Paul F. Cook, (Goodlad, 1994). Margaret F. Ostlund, Historically, school reforms and teacher educa and James R. Young are tion innovations have often fallen short of expecta faculty members in the tion because classroom teachers, teacher educators Department of Teacher and their undergraduate students do not typically Education, Brigham possess a collaborative vision or shared language Young University, Provo, concerning teaching, learning, and teacher educa Utah. tion. Accordingly, the problems that exist for both
Nonhematopoietic Gastrointestinal Neoplasia in Cats: A Retrospective Study of 44 Cases

Gastrointestinal neoplasms other than lymphosarcomas and mast cell tumors were diagnosed in 44 cats during a 14-year period at the Washington Animal Disease Diagnostic Laboratory. All the tumors were malignant; 31 metastasized or recurred. One cat had fibrosarcoma; another, leiomyosarcoma. The other 42 cats had adenocarcinomas, which were subclassified into three histologic patterns: tubular adenocarcinoma; undifferentiated carcinoma; and mucinous adenocarcinoma. The cats averaged 10.6 years of age. There was no sex predisposition. Siamese cats had a higher frequency of adenocarcinomas than other breeds. Osseous and chondroid metaplasia occurred in nine adenocarcinomas.
On Origins of Bubbles

We discuss – in what is intended to be a pedagogical fashion – a criterion, which is a lower bound on a certain ratio, for when a stock (or a similar instrument) is not a good investment in the long term, which can happen even if the expected return is positive. The root cause is that prices are positive and have skewed, long-tailed distributions, which coupled with volatility results in a long-run asymmetry. This relates to bubbles in stock prices, which we discuss using a simple binomial tree model, without resorting to the stochastic calculus machinery. We illustrate empirical properties of the aforesaid ratio. Log of market cap and sectors appear to be relevant explanatory variables for this ratio, while price-to-book ratio (or its log) is not. We also discuss a short-term effect of volatility, to wit, the analog of Heisenberg’s uncertainty principle in finance and a simple derivation thereof using a binary tree.
Factors Associated with Development of Speech Production Skills in Children Implanted by Age Five

Objective This study investigated speech production outcomes and the factors influencing the outcomes in children who had 4 to 6 yr of experience with a multichannel cochlear implant. Production variables examined included speech intelligibility, accuracy of consonant and vowel production, percentage of plosives and fricatives produced, duration of sentences, percentage of time involved in communication breakdowns during a communication sample, and responses to a speech usage questionnaire. Design 181 children between the ages of 8 and 9 yr who received a multichannel cochlear implant before age 5 yr participated as subjects. Independent variables were the amount and type of educational intervention and intervening variables were distributed across child, family and implant characteristics. Multiple regression analyses provided a measure of the amount of variance associated with speech production skills accounted for by the intervening and independent variables. Results Performance for the key words in the speech intelligibility measured averaged 63.5% for the group of children. Accuracy of phoneme production was higher for consonants (68.0%) than for vowels (61.6%) for the group. More plosives were present for acoustic analyses (91.6%) than were fricatives (78.4%). Duration for the speech intelligibility sentences averaged 2572.3 msec. Communication breakdowns occurred on average 14.5% of the time involved in a language sample. Significant predictors of high levels of oral communication skills included higher nonverbal intelligence, gender, longer use of SPEAK processing strategy, a fully active electrode array, greater dynamic range, and greater growth of loudness. The primary rehabilitative factors contributing to high levels of oral communication were an emphasis on oral-aural communication and classrooms that emphasized dependence on speech and listening. Conclusions Speech production performance in children with cochlear implants is influenced by nonverbal intelligence, gender, implant characteristics including the length of time using the newest speech processing strategies, and educational programs emphasizing oral-aural communication. Factors previously thought to be major contributors to speech production performance, such as age of onset of deafness and age of implantation, did not appear to play significant roles in predicting levels of speech production performance.
Quantitative Risk Assessment for African Horse Sickness in Live Horses Exported from South Africa

African horse sickness (AHS) is a severe, often fatal, arbovirus infection of horses, transmitted by Culicoides spp. midges. AHS occurs in most of sub-Saharan Africa and is a significant impediment to export of live horses from infected countries, such as South Africa. A stochastic risk model was developed to estimate the probability of exporting an undetected AHS-infected horse through a vector protected pre-export quarantine facility, in accordance with OIE recommendations for trade from an infected country. The model also allows for additional risk management measures, including multiple PCR tests prior to and during pre-export quarantine and optionally during post-arrival quarantine, as well as for comparison of risk associated with exports from a demonstrated low-risk area for AHS and an area where AHS is endemic. If 1 million horses were exported from the low-risk area with no post-arrival quarantine we estimate the median number of infected horses to be 5.4 (95% prediction interval 0.5 to 41). This equates to an annual probability of 0.0016 (95% PI: 0.00015 to 0.012) assuming 300 horses exported per year. An additional PCR test while in vector-protected post-arrival quarantine reduced these probabilities by approximately 12-fold. Probabilities for horses exported from an area where AHS is endemic were approximately 15 to 17 times higher than for horses exported from the low-risk area under comparable scenarios. The probability of undetected AHS infection in horses exported from an infected country can be minimised by appropriate risk management measures. The final choice of risk management measures depends on the level of risk acceptable to the importing country.
Estimates of total body iron indicate 19 mg and 38 mg oral iron are equivalent for the mitigation of iron deficiency in individuals experiencing repeated phlebotomy

Iron deficiency anemia is a common clinical condition often treated with tablets containing 65 mg of elemental iron. Such doses can elicit gastrointestinal side effects lowering patient compliance. Oral iron supplements also increase hepcidin production causing decreased fractional absorption of subsequent doses. Frequent blood donors often become iron deficient. Therefore, they were enrolled in a two‐year study involving continued blood donations and randomization to receive no pill, placebo, 19, or 38 mg ferrous gluconate for 60 days. Total body iron (TBI) did not change for the subset of donors in the no pill and placebo groups who completed both enrollment and final visits (P = .21 and P = .28, respectively). However, repeated measures regression analysis on the complete dataset estimated a significant decrease in TBI of 52 mg/year for the placebo and no pill groups (P = .001). The effects of 19 and 38 mg iron supplementation on TBI were indistinguishable (P = .54). TBI increased by 229 mg after the initial 60 days of iron supplementation (P < .0001) and was maintained at this higher level with continued iron supplementation following each subsequent donation. The TBI increase was apportioned 51 mg to red cell iron (P < .0001) and 174 mg to storage iron (P < .0001). Changes in storage iron were negatively impacted by 57 mg due to concurrent antacid use (P = .04). These findings in blood donors suggest that much lower doses of iron than are currently used will be effective for clinical treatment of iron deficiency anemia.