Miracle-dz/UniFAR-FTUs huggingface dataset:
Pursuit of Complementary and Alternative Medicine Treatments in Adolescents With Cerebral Palsy

This study determined the extent to which parents of adolescents with cerebral palsy seek out complementary and alternative medicine services. A regional sample of 166 adolescents (15.5 ± 2.4 years) with cerebral palsy were recruited. Parents completed a questionnaire identifying the complementary and alternative medicine services received over the past year. Most (73.2%) did not currently utilize any of the listed services; 7.3% used 2 or more services. The most commonly used services were massage (15.4%), hyperbaric oxygen (9.6%), and osteopathy (5.7%). Youth with limited hand function were more likely (P = .01) to undergo hyperbaric oxygen. Massage therapy services were more frequent in youth with greater activity limitations (P < .005). Sociodemographic factors were not predictive of use. Approximately one quarter of families sought out these services for their adolescents with cerebral palsy. Many are expensive privately funded treatments. Physicians should openly discuss these options with families, highlighting the current state of knowledge on their efficacy.
Bayesian estimation of neural activity for non stationary sources using time frequency based priors

Electroencephalographic (EEG) recordings contain dynamic information inherent to its complex behavior, therefore, the accurate estimation of neural activity is highly dependent on the inclusion of such information in the inverse problem solution. The present work presents a way to obtain constraints for the Bayesian inverse problem solution, through a Variational Bayes Approach, using information contained in the space-time-frequency, more specifically under the Automatic Relevance Determination (ARD) framework. The time-frequency representation of the EEG allows to extract information that could be hidden in the nonstationarities and noise that are usually present in EEG data. The performance of the proposed method is evaluated using simulated EEG data under several SNRs in terms of spatial accuracy, temporal accuracy and mean squared error. Obtained results show that the proposed approach improves the spatial accuracy of the inversion under low SNR-data i.e., it is more robust to noise. Nevertheless, it does not show any improvement in the temporal accuracy of the estimations. Furthermore, the mean squared error does not show any significant result to assess the performance of the inversions.
Inhibition of rRNA synthesis by poliovirus: specific inactivation of transcription factors

Synthesis of rRNA by RNA polymerase I is almost completely inhibited soon after infection of human cells with poliovirus. We show that extracts prepared from poliovirus-infected HeLa cells are severely inhibited in their ability to transcribe from a human rDNA promoter compared with extracts from mock-infected cells. Two lines of evidence presented here suggest that a specific transcriptional activity required for rDNA transcription in vitro is impaired in virus-infected cells. First, fractionation of individual transcriptional components by phosphocellulose chromatography and subsequent reconstitution experiments showed that the specific transcriptional activity of fraction C (0.8 M KCl eluate) from virus-infected cells was reduced three- to fourfold relative to that isolated from mock-infected cells. The activities of other transcription factors needed for in vitro transcription from the rDNA promoter were unaffected. Second, fraction C derived from mock-infected cells specifically restored transcription in extracts prepared from virus-infected cells. Fraction C contained both a nonspecific RNA polymerase I elongation activity and a specific factor activity which was needed for accurate transcription initiation. It is the specific transcriptional activity and not the nonspecific chain elongation activity of fraction C that is affected in cells infected with poliovirus.
Positive Affect Predicts Lower Risk of AIDS Mortality

Objective: The objective of this study was to test the association of positive affect as measured by the Center for Epidemiologic Studies Depression Scale (CES‐D) with risk of AIDS mortality, controlling for the other CES‐D subscales and laboratory measures of disease progression. Methods: Data come from the San Francisco Men's Health Study, a prospective study of a household probability sample of single men in San Francisco. The subjects were 407 men who were HIV+ at study baseline. Results: In time‐dependent Cox proportional hazards models, the positive affect subscale of the CES‐D was significantly associated with lower risk of AIDS mortality (RR = 0.89, CI = 0.84‐0.95). When risk estimates were adjusted for time‐dependent covariates of CD4, serum &bgr;2‐microglobulin, P24 antigen, antiretroviral use, and the other subscales of the CES‐D, positive affect remained significantly predictive of lower risk of AIDS mortality (RR = 0.90, CI = 0.85‐0.97). When the CES‐D subscale predictors were lagged by 12, 24, and 36 months in order to address the possibility that positive affect was simply a marker for better health, positive affect remained significantly predictive lagged by 12 months and marginally predictive lagged by 24 months. Conclusions: Positive affect seems to be the “active ingredient” in the association of scores on the CES‐D depressive mood scale and mortality in this sample of HIV+ men. Future work should expand the traditional negative‐affect‐only focus to encompass the significant role that positive affect plays in living with HIV.
Molecular determinants of terminal growth arrest induced in tumor cells by a chemotherapeutic agent

Treatment with chemotherapy or radiation is not invariably cytotoxic to all tumor cells. Some of the cells that survive treatment recover and resume proliferation, whereas others undergo permanent growth arrest. To understand the nature of treatment-induced terminal growth arrest, colon carcinoma cells were exposed to doxorubicin, and surviving cells were separated into proliferating and growth-arrested populations. Only growth-arrested cells displayed phenotypic markers of cell senescence and failed to form colonies. Gene expression was compared between senescent and proliferating fractions of drug-treated cells by using cDNA microarray hybridization and reverse transcription–PCR. Drug-induced senescence was associated with inhibition of genes involved in cell proliferation and with coinduction of multiple intracellular and secreted growth inhibitors. Several tumor suppressors and other genes that are down-regulated in carcinogenesis were up-regulated in senescent tumor cells. Induction of most growth inhibitors was delayed but not abolished in cells with homozygous knockout of p53, in agreement with only limited p53 dependence of drug-induced terminal growth arrest. On the other hand, senescent cells overexpressed secreted proteins with antiapoptotic, mitogenic, and angiogenic activities, suggesting that drug-induced senescence is associated with paracrine tumor-promoting effects. About one-third of the genes up-regulated in senescent cells and almost all of the down-regulated genes showed decreased or delayed changes in p21Waf1/Cip1/Sdi1-deficient cells, indicating that p21 is a major mediator of the effects of p53 on gene expression. Elucidation of molecular changes in tumor cells that undergo drug-induced senescence suggests potential strategies for diagnostics and therapeutic modulation of this antiproliferative response in cancer treatment.
Evolutionary Diversification of Alanine Transaminases in Yeast: Catabolic Specialization and Biosynthetic Redundancy

Gene duplication is one of the major evolutionary mechanisms providing raw material for the generation of genes with new or modified functions. The yeast Saccharomyces cerevisiae originated after an allopolyploidization event, which involved mating between two different ancestral yeast species. ScALT1 and ScALT2 codify proteins with 65% identity, which were proposed to be paralogous alanine transaminases. Further analysis of their physiological role showed that while ScALT1 encodes an alanine transaminase which constitutes the main pathway for alanine biosynthesis and the sole pathway for alanine catabolism, ScAlt2 does not display alanine transaminase activity and is not involved in alanine metabolism. Moreover, phylogenetic studies have suggested that ScALT1 and ScALT2 come from each one of the two parental strains which gave rise to the ancestral hybrid. The present work has been aimed to the understanding of the properties of the ancestral type Lacchancea kluyveri LkALT1 and Kluyveromyces lactis KlALT1, alanine transaminases in order to better understand the ScALT1 and ScALT2 evolutionary history. These ancestral -type species were chosen since they harbor ALT1 genes, which are related to ScALT2. Presented results show that, although LkALT1 and KlALT1 constitute ScALT1 orthologous genes, encoding alanine transaminases, both yeasts display LkAlt1 and KlAlt1 independent alanine transaminase activity and additional unidentified alanine biosynthetic and catabolic pathway(s). Furthermore, phenotypic analysis of null mutants uncovered the fact that KlAlt1 and LkAlt1 have an additional role, not related to alanine metabolism but is necessary to achieve wild type growth rate. Our study shows that the ancestral alanine transaminase function has been retained by the ScALT1 encoded enzyme, which has specialized its catabolic character, while losing the alanine independent role observed in the ancestral type enzymes. The fact that ScAlt2 conserves 64% identity with LkAlt1 and 66% with KlAlt1, suggests that ScAlt2 diversified after the ancestral hybrid was formed. ScALT2 functional diversification resulted in loss of both alanine transaminase activity and the additional alanine-independent LkAlt1 function, since ScALT2 did not complement the Lkalt1Δ phenotype. It can be concluded that LkALT1 and KlLALT1 functional role as alanine transaminases was delegated to ScALT1, while ScALT2 lost this role during diversification.
Subcortical White Matter Metabolic Changes Remote from Focal Hemorrhagic Lesions Suggest Diffuse Injury after Human Traumatic Brain Injury

OBJECTIVE:We used positron emission tomographic studies to prospectively examine the relationship between glucose and oxidative metabolism in the subcortical white matter (WM) acutely after traumatic brain injury (TBI). The objective was to determine the nature, extent, and degree of metabolic abnormalities in subcortical brain regions remote from hemorrhagic lesions. METHODS:Sixteen normal volunteers and 10 TBI patients (Glasgow Coma Scale score, 4–10; age, 17–64 yr; 6 with focal and 4 with diffuse injury) were studied. Each subject underwent dynamic positron emission tomographic studies using [15O]CO, 15O2, [15O]H2O, and fluorodeoxyglucose plus a magnetic resonance imaging scan acutely after TBI. Parametric images of the metabolic rate of oxygen and metabolic rate of glucose were generated, and a molar oxygen-to-glucose utilization ratio was calculated. Data from gray matter and WM remote from hemorrhagic lesions, plus whole brain, were analyzed. RESULTS:There was a significant reduction in the subcortical WM oxygen-to-glucose utilization ratio after TBI compared with normal values (3.99 ± 0.77 versus 5.37 ± 1.00; P < 0.01), whereas the mean cortical gray matter and whole-brain values remained unchanged. WM metabolic changes, which were diffuse throughout the hemispheres, were characterized by a reduction in the metabolic rate of oxygen without a concomitant drop in the metabolic rate of glucose. CONCLUSION:The extent and degree of subcortical WM metabolic abnormalities after moderate and severe TBI suggest that diffuse WM injury is a general phenomenon after such injuries. This pervasive finding may indicate that the concept of focal traumatic injury, although valid from a computed tomographic imaging standpoint, may be misleading when considering metabolic derangements associated with TBI.
Factors that affect action possibility judgements: Recent experience with the action and the current body state

It has been suggested that action possibility judgements are formed through a covert simulation of the to-be-executed action. We sought to determine whether the motor system (via a common coding mechanism) influences this simulation, by investigating whether action possibility judgements are influenced by experience with the movement task (Experiments 1 and 2) and current body states (Experiment 3). The judgement task in each experiment involved judging whether it was possible for a person's hand to accurately move between two targets at presented speeds. In Experiment 1, participants completed the action judgements before and after executing the movement they were required to judge. Results were that judged movement times after execution were closer to the actual execution time than those prior to execution. The results of Experiment 2 suggest that the effects of execution on judgements were not due to motor activation or perceptual task experience—alternative explanations of the execution-mediated judgement effects. Experiment 3 examined how judged movement times were influenced by participants wearing weights. Results revealed that wearing weights increased judged movement times. These results suggest that the simulation underlying the judgement process is connected to the motor system, and that simulations are dynamically generated, taking into account recent experience and current body state.
Activity-Dependent Codevelopment of the Corticospinal System and Target Interneurons in the Cervical Spinal Cord

Corticospinal tract (CST) connections to spinal interneurons are conserved across species. We identified spinal interneuronal populations targeted by the CST in the cervical enlargement of the cat during development. We focused on the periods before and after laminar refinement of the CST terminations, between weeks 5 and 7. We used immunohistochemistry of choline acetyltransferase (ChAT), calbindin, calretinin, and parvalbumin to mark interneurons. We first compared interneuron marker distribution before and after CST refinement. ChAT interneurons increased, while calbindin interneurons decreased during this period. No significant changes were noted in parvalbumin and calretinin. We next used anterograde labeling to determine whether the CST targets different interneuron populations before and after the refinement period. Before refinement, the CST terminated sparsely where calbindin interneurons were located and spared ChAT interneurons. After refinement, the CST no longer terminated in calbindin-expressing areas but did so where ChAT interneurons were located. Remarkably, early CST terminations were dense where ChAT interneurons later increased in numbers. Finally, we determined whether corticospinal system activity was necessary for the ChAT and calbindin changes. We unilaterally inactivated M1 between weeks 5 and 7 by muscimol infusion. Inactivation resulted in a distribution of calbindin and ChAT in spinal gray matter regions where the CST terminates that resembled the immature more than mature pattern. Our results show that the CST plays a crucial role in restructuring spinal motor circuits during development, possibly through trophic support, and provide strong evidence for the importance of connections with key spinal interneuron populations in development of motor control functions.
Gain enhancement of a dual feed microstrip array antenna using parasitic elements

In this paper, an in-phase/anti-phase dual feed microstrip array antenna with parasitic elements is proposed to improve the antenna's gain and it is experimentally evaluated. The proposed antenna provides various kinds of functionality such as direction-of-arrival estimation, beam steering and polarization switching with a simple structure. Better than 2-dB gain enhancement is expected by using the parasitic elements. The separation and height of the parasitic elements are also discussed. The separation of 0. 8λ0 with height of 0. 5λ0 provides the highest gain for both the in-phase and anti-phase feeds.
Genome-wide technology for determining RNA stability in mammalian cells

Changing the abundance of transcripts by regulated RNA degradation is a critical step in the control of various biological pathways. Recently, genome-wide inhibitor-free technologies for determining RNA stabilities in mammalian cells have been developed. In these methods, endogenous RNAs are pulse labeled by uridine analogs [e.g., 4-thiouridine (4sU), 5-etyniluridine (EU) and 5′-bromo-uridine (BrU)], followed by purification of labeled de novo RNAs. These technologies have revealed that the specific half-life of each mRNA is closely related to its physiological function. Genes with short-lived mRNAs are significantly enriched among regulatory genes, while genes with long-lived mRNAs are enriched among housekeeping genes. This review describes the recent progress of experimental procedures for measuring RNA stability.
Metabolic activation of a pentafluorophenylethylamine derivative: Formation of glutathione conjugates in vitro in the rat

The aim was to investigate the metabolic activation potential of a pentafluorophenylethylamine derivative (compound I) in vitro in the rat and to identify the cytochrome P450 (CYP) enzymes that catalyse these metabolic activation processes. Reduced glutathione (GSH) was fortified in rat hepatocytes and liver microsomes to trap possible reactive intermediates. Four glutathione conjugates (M1–4) were identified by LC-MSn following incubation of compound I in GSH-enriched rat hepatocytes and liver microsomes. Three of these conjugates (M2–4) have not been reported previously for pentafluorophenyl derivatives. Elemental composition analysis of these conjugates was obtained using high-resolution quadrupole time-of-flight mass spectrometry. The formation of GSH conjugate M1 was rationalized as a direct nucleophilic replacement of fluoride by glutathione, whereas the formation of the GSH conjugates M2–4 was proposed to occur by NADPH-dependent metabolic activation of the pentafluorophenyl ring via arene oxide, quinone and/or quinoneimine reactive intermediates. Formation of these conjugates was enhanced three- to five-fold in liver microsomes obtained from phenobarbital- and dexamethasone-treated rats. In incubations with pooled rat liver microsomes and recombinant rat CYP3A1 and CYP3A2, troleandomycin (TAO) reduced the formation of GSH conjugates M2–4 by 80–90%, but it had no effect on the formation of M1. Incubation of compound I with rat supersomes indicated that only CYP3A1 and CYP3A2 were capable of mediating these metabolic activation processes.
U.S. Cambodian refugees' use of complementary and alternative medicine for mental health problems.

OBJECTIVE This study examined U.S. Cambodian refugees' use of complementary and alternative medicine and Western sources of care for psychiatric problems. Analyses assessed the extent to which complementary and alternative medicine was used in the absence of Western mental health treatment and whether use of complementary and alternative medicine was associated with decreased use of Western services. METHODS Face-to-face interviews were conducted with a representative sample drawn from the largest Cambodian refugee community in the United States. The sample included 339 persons who met criteria in the past 12 months for posttraumatic stress disorder, major depression, or alcohol use disorder. Respondents described contact with complementary and alternative medicine and Western service providers for psychological problems in the preceding 12 months. Bivariate and multivariate logistic regression analyses were used. RESULTS Seventy-two percent of the sample sought Western mental health services, and 34% relied on complementary and alternative medicine in the past year. Seeking complementary and alternative medicine was strongly and positively associated with seeking Western services, contrary to the hypothesis that use of complementary and alternative medicine inhibits seeking Western mental health treatment. CONCLUSIONS Only a small percentage of Cambodian refugees used complementary and alternative medicine exclusively (5%), and utilization of complementary and alternative medicine was positively associated with seeking Western sources of care for mental health problems. Complementary and alternative medicine use does not appear to be a significant barrier to mental health treatment in this population, contrary to the Surgeon General's conclusion that Asian Americans' use of alternative resources may inhibit their utilization of Western mental health care.
Electronic continuing education in the health professions: An update on evidence from RCTs

Introduction: Demonstrating the effectiveness of the rapidly expanding field of electronic continuing education (e‐CE) has important implications for CE in the health professions. This study provides an update on evidence from randomized controlled trials (RCTs) assessing the effectiveness of e‐CE in the health professions. Methods: A literature search of RCTs was performed in MEDLINE, EMBASE, and CINAHL from 2004 to 2007. Papers were reviewed separately by 2 of the authors and results were categorized and reviewed according to study comparisons. Results: Fifteen studies met our inclusion criteria. Six compared e‐CE to no intervention or placebo. Of these 6 studies, 4 showed a statistically significant advantage of the e‐CE intervention and 2 showed no significant effect. Two studies compared e‐CE to a lecture. Of these, 1 showed an advantage of e‐CE and 1 showed no difference. Two studies compared e‐CE to a small‐group interactive intervention. In both studies, the e‐CE group outperformed the control. Two studies compared a multicomponent e‐CE intervention to one based on flat text, and both showed the multicomponent intervention to be more effective. Two of the 15 studies demonstrated a statistically significant effect on practice patterns. Positive effects of e‐CE on knowledge were shown to persist for up to 12 months and effects on practice up to 5 months. Discussion: Overall, these studies suggest that multicomponent e‐CE interventions can be effective in changing health professionals' practice patterns, and improve their knowledge. E‐CE interventions based purely on flat text appear to be of limited effectiveness in changing either knowledge or practice. These results support the use of multicomponent e‐CE as a method of CE delivery.
Negative Word Production in Adults With Right Hemisphere Brain Damage: Effects of Implicit Assessment and Contextual Bias.

PURPOSE Both theory and evidence suggest that unilateral right hemisphere brain damage (RHD) should impair the processing of negative emotions. Typical metalinguistic assessments, however, may obscure processing strengths. This study investigated whether adults with RHD would produce proportionately fewer negative emotion words than control participants in an implicit assessment task and whether a negatively toned contextual bias would enhance performance. METHODS Eleven participants with RHD and 10 control participants without brain damage watched a video in 2 parts and described each segment. Between segments, participants evaluated the emotion conveyed by sentences designed to induce the negative bias. RESULTS The primary outcome measure, percentage of negative emotion words in video descriptions, did not differ between groups. After the contextual bias, this measure significantly increased for both groups, whereas production of motion words, a control variable, remained constant. CONCLUSIONS Findings are consistent with a view that attributes some deficient RHD performances to the nature and/or demands of explicit metalinguistic assessment tasks. These results call for modulation of prevailing hypotheses that attribute negative emotion processing as an undifferentiated whole solely to the right cerebral hemisphere. The results also further substantiate the rationale of an experimental treatment that exploits contextual bias and priming for individuals with RHD.