Miracle-dz/UniFAR-FTUs huggingface dataset:
What do excellent psychotherapy supervisors do?

OBJECTIVE The author's goal was to discover strategies used by psychotherapy supervisors judged to be excellent teachers. METHOD In an earlier study, experienced teachers of psychotherapy rated the level of excellence of 34 different supervisors in 53 videotaped supervision sessions. In this study, the authors examined the transcripts of the nine videotapes assigned the highest ratings as well as three videotapes assigned mid-level ratings and three videotapes assigned low ratings in the previous study. In analyzing these transcripts, the authors drew from their experience with the complete set of videotapes. RESULTS Supervisors with high ratings allowed the resident's story about the encounter with the patient to develop. They consistently tracked the most immediate aspects of the resident's affectively charged concerns. Most of their comments were directed toward helping the resident further understand the patient and were specific to the material presented in the session. The resident was invited to speculate about the material, and technical words were used sparsely. Discussions about the relationships between resident and patient and between supervisor and resident were in the context of the resident's concerns. Supervisors with mid-level ratings were less disciplined in tracking the resident's concerns and inhibited the development of the resident's story. Supervisors with low ratings paid little or no attention to the resident's issues. CONCLUSIONS The ability to track residents' concerns is at the center of supervisory activities rated as excellent. The resident provides data about what occurred, and new knowledge is constructed in the supervisory interaction. These findings provide an empirical basis for orienting supervisors to supervision.
Research of Struts2 framework and web application based on Ajax

The respond of traditional web application to user interface is slow and wasting bandwidth, which bring user a lot of inconvenience, while the emergence of Ajax has changed this situation. Ajax can maintain data without refreshing the page, which greatly reduce the time of waiting for a large number of exchanged data for user. The advantages of both is fully demonstrated by the support which Struts2 on Ajax. This paper combines the technology of Ajax and the framework of Struts2, elaborating the principles of both, and through campus-based BBS system propose Struts2 framework and application based on Ajax.
Ectopic SOX9 Mediates Extracellular Matrix Deposition Characteristic of Organ Fibrosis*

Appropriate temporospatial expression of the transcription factor SOX9 is important for normal development of a wide range of organs. Here, we show that when SOX9 is expressed ectopically, target genes become expressed that are associated with disease. Histone deacetylase inhibitors in clinical trials for cancer therapy induced SOX9 expression via enhanced recruitment of nuclear factor Y (NF-Y) to CCAAT elements in the SOX9 proximal promoter. The effect of histone deacetylase inhibitors could be elicited in cells that normally lack SOX9, such as hepatocytes. In human fetal hepatocytes, this aberrant induction of SOX9 protein caused ectopic expression of COL2A1 and COMP1 that encode extracellular matrix (ECM) components normally associated with chondrogenesis. Previously, ectopic expression of this “chondrogenic” profile has been implicated in vascular calcification. More broadly, inappropriate ECM deposition is a hallmark of fibrosis. We demonstrated that induction of SOX9 expression also occurred during activation of fibrogenic cells from the adult liver when the transcription factor was responsible for expression of the major component of fibrotic ECM, type 1 collagen. These combined data identify new aspects in the regulation of SOX9 expression. They support a role for SOX9 beyond normal development as a transcriptional regulator in the pathology of fibrosis.
High-frequency generator based on pulsed excitation of the oscillating circuit for biological decontamination

Within this research the damping oscillation pulses solid-state generator based on the pulsed excitation of the oscillating circuit has been developed and experimentally studied. Output harmonic voltage oscillations frequency reaches a few megahertz, while the pulse repetition rate is up to 1 kHz. Output pulse repetition rate of the generator defines power consumption from the supply main, which does not exceed 700 W, at the same time maximum peak power of the output pulses reaches several hundred kilowatts. A high-frequency transformer is used in the output stage of the generator. Thanks to using the transformer it is possible to match an impedance of the generator with different loads by simple changing of the windings rate. In present research the generator was used for excitation of a corona and DBD discharges in air, created for surface decontamination. Within this paper the electrical circuit diagram and principle of the generator operation as well as surface decontamination experimental results are described.
Using microRNA profiling in urine samples to develop a non‐invasive test for bladder cancer

Current standard methods used to detect and monitor bladder urothelial cell carcinoma (UCC) are invasive or have low sensitivity. The incorporation into clinical practice of a non‐invasive tool for UCC assessment would enormously improve patients' quality of life and outcome. This study aimed to examine the microRNA (miRNA) expression profiles in urines of UCC patients in order to develop a non‐invasive accurate and reliable tool to diagnose and provide information on the aggressiveness of the tumor. We performed a global miRNA expression profiling analysis of the urinary cells from 40 UCC patients and controls using TaqMan® Human MicroRNA Array followed by validation of 22 selected potentially diagnostic and prognostic miRNAs in a separate cohort of 277 samples using a miRCURY LNA™ qPCR system. miRNA‐based signatures were developed by multivariate logistic regression analysis and internally cross‐validated. In the initial cohort of patients, we identified 40 and 30 aberrantly expressed miRNA in UCC compared with control urines and in high compared with low grade tumors, respectively. Quantification of 22 key miRNAs in an independent cohort resulted in the identification of a six miRNA diagnostic signature with a sensitivity of 84.8% and specificity of 86.5% (AUC = 0.92) and a two miRNA prognostic model with a sensitivity of 84.95% and a specificity of 74.14% (AUC = 0.83). Internal cross‐validation analysis confirmed the accuracy rates of both models, reinforcing the strength of our findings. Although the data needs to be externally validated, miRNA analysis in urine appears to be a valuable tool for the non‐invasive assessment of UCC.
Molecular Pathogenesis of Genetic and Inherited Diseases Critical Roles of Lysosomal Acid Lipase in Myelopoiesis

Lysosomal acid lipase (LAL) is a key enzyme that cleaves cholesteryl esters and triglycerides to generate free fatty acids and cholesterol in lysosomes. Genetic ablation of the lal gene (lal / ) in mice has resulted in a systemic increase of macrophages and neutrophils, causing severe inflammation and pathogenesis in multiple organs. We hypothesized that aberrant growth and differentiation of myeloid cells in lal / mice arises from dysregulated production of progenitor cells in the bone marrow. Indeed, lal / mice displayed increased numbers of primitive lin Sca-1 c-Kit (LSK) cells and granulocyte-macrophage precursors (GMP). Increased high proliferative potential colony-forming cells (HPP-CFC) were enumerated from cultured lal / bone marrow cells, as were significantly more CFU-GM, CFU-G, and CFU-M colonies. As a consequence, lal / mice developed significant myeloid infiltration, particularly with CD11b /Gr-1 myeloid-derived suppressive cells in multiple organs. Both decreased apoptosis and increased proliferation contribute to the systemic increase of myeloid cells in lal / myeloid cells. These lal / CD11b /Gr-1 cells displayed suppressive activity on T cell proliferation and function in vitro. Bone marrow chimeras confirmed that the myeloproliferative disorder in lal / mice was primarily attributable to autonomous defects in myeloid progenitor cells , although the hematopoietic microenvironment in the lal / mice did not support hematopoiesis normally. These results provide evidence that LAL is an important regulator of myelopoiesis during hematopoietic development , differentiation, and homeostasis. (Am J Pathol 2010, 176:2394–2404; DOI: 10.2353/ajpath.2010.091063)
Molecular interactions of alanine-rich and proline-rich regions of cell surface protein antigen c in Streptococcus mutans.

INTRODUCTION Streptococcus mutans has been implicated as a primary causative agent of dental caries in humans, and its cell surface protein antigen c (PAc) is known to be associated with sucrose-independent adhesion to tooth surfaces. PAc is composed of several domains, including an N-terminal signal sequence, an alanine-rich repeat region (A-region), a proline-rich repeat region (P-region), and an anchor region. METHODS To investigate the functions of each domain, an A-region-deficient mutant strain of S. mutans was constructed, and recombinant PAc and A- and P-region proteins were also constructed. The interactions of each domain with the recombinant proteins were analyzed using surface plasmon resonance spectroscopy with a biomolecular interaction analyzing system. RESULTS The A-region-deficient mutant strain showed the lowest levels of adherence to saliva-coated hydroxyapatite. Furthermore, findings in an immunoblot assay indicated that the A-region protein reacted strongly with proline-rich proteins in saliva, while the recombinant P-region protein interacted more quickly with PAc than the recombinant A-region protein. CONCLUSION These results suggest that the A-region has a strong relationship with adhesion to tooth surfaces, while the P-region has a high affinity for PAc.
Pain and pain management in newborn infants: a survey of physicians and nurses.

BACKGROUND Despite an increased awareness among clinicians regarding pain and pain management for infants undergoing surgery, pain associated with procedures performed outside the operating room may not be adequately managed. PURPOSE To examine the beliefs and self-described behavior of physicians and nurses regarding the management of procedural pain in newborn infants. METHODS A survey was distributed to 467 clinicians (nurses and physicians) working in 11 level II and 4 level III nurseries in a large metropolitan area. Respondents were asked to rate the painfulness of 12 common bedside nursery procedures and how often pharmacologic and nonpharmacologic (comfort) measures are currently used and should be used for those procedures. Demographic data were also collected. RESULTS Surveys were completed by 374 clinicians (80% response rate). Physicians and nurses believe infants feel as much pain as adults and that 9 of the 12 listed procedures are moderately to very painful. Neither pharmacologic nor comfort measures are believed to be used frequently, even for the most painful procedures. Physicians and nurses believe both pharmacologic and comfort measures should be used more frequently, but nurses believe comfort measures should be used more frequently than do physicians. Beliefs about infant pain and procedural pain were related to pain management preferences. Physicians' but not nurses' ratings were associated with significant personal pain. CONCLUSIONS Despite their beliefs that infants experience significant procedure-related pain, clinicians believe pain management for infants remains below optimal levels. Barriers to more consistent and effective pain management need to be identified and surmounted.
A Lightweight Malware Classification Method Based on Detection Results of Anti-Virus Software

With the development of cyber threats on the Internet, the number of malware, especially unknown malware, is also dramatically increasing. Since all of malware cannot be analyzed by analysts, it is very important to find out new malware that should be analyzed by them. In order to cope with this issue, the existing approaches focused on malware classification using static or dynamic analysis results of malware. However, the static and the dynamic analyses themselves are also too costly and not easy to build the isolated, secure and Internet-like analysis environments such as sandbox. In this paper, we propose a lightweight malware classification method based on detection results of anti-virus software. Since the proposed method can reduce the volume of malware that should be analyzed by analysts, it can be used as a preprocess for in-depth analysis of malware. The experimental showed that the proposed method succeeded in classification of 1,000 malware samples into 187 unique groups. This means that 81% of the original malware samples do not need to analyze by analysts.
A liquid chromatographic-tandem mass spectrometric method for determination of artemether and its metabolite dihydroartemisinin in human plasma.

BACKGROUND Artemether-lumefantrine is the most widely recommended artemisinin-based combination treatment for falciparum malaria. Quantification of artemether and its metabolite dihydroartemisinin in biological matrices has traditionally been difficult, with sensitivity being an issue. RESULTS A high-throughput bioanalytical method for the analysis of artemether and its metabolite dihydroartemisinin in human plasma using solid-phase extraction in the 96-well plate format and liquid chromatography coupled to positive ion mode tandem mass spectroscopy has been developed and validated according to US FDA guidelines. The method uses 50 µl plasma and covers the calibration range 1.43-500 ng/ml with a limit of detection at 0.36 ng/ml. CONCLUSIONS The developed liquid chromatography-tandem mass spectrometry assay is more sensitive than all previous methods despite using a lower plasma volume (50 µl) and is highly suitable for clinical studies where plasma volumes are limited, such as pediatric trials.
Advanced Multi-Axis Spine Testing: Clinical Relevance and Research Recommendations

Back pain and spinal degeneration affect a large proportion of the general population. The economic burden of spinal degeneration is significant, and the treatment of spinal degeneration represents a large proportion of healthcare costs. However, spinal surgery does not always provide improved clinical outcomes compared to non-surgical alternatives, and modern interventions, such as total disc replacement, may not offer clinically relevant improvements over more established procedures. Although psychological and socioeconomic factors play an important role in the development and response to back pain, the variation in clinical success is also related to the complexity of the spine, and the multi-faceted manner by which spinal degeneration often occurs. The successful surgical treatment of degenerative spinal conditions requires collaboration between surgeons, engineers, and scientists in order to provide a multi-disciplinary approach to managing the complete condition. In this review, we provide relevant background from both the clinical and the basic research perspectives, which is synthesized into several examples and recommendations for consideration in increasing translational research between communities with the goal of providing improved knowledge and care. Current clinical imaging, and multi-axis testing machines, offer great promise for future research by combining invivo kinematics and loading with in-vitro testing in six degrees of freedom to offer more accurate predictions of the performance of new spinal instrumentation. Upon synthesis of the literature, it is recommended that in-vitro tests strive to recreate as many aspects of the in-vivo environment as possible, and that a physiological preload is a critical factor in assessing spinal biomechanics in the laboratory. A greater link between surgical procedures, and the outcomes in all three anatomical planes should be considered in both the in-vivo and in-vitro settings, to provide data relevant to quality of motion, and stability.
Direct 3-D shape recovery from image sequence based on multi-scale Bayesian network

We propose a new method for recovering a 3-D object shape from an image sequence. In order to recover high-resolution relative depth without using the complex Markov random field (MRF) that includes a line process, we construct a recovery algorithm based on a belief propagation scheme using a multi-scale Bayesian network. With this algorithm, relative 3-D motion between a camera and an object can be determined together with relative depth, and the maximum a posteriori expectation-maximization (MAP-EM) algorithm is effectively used to determine a suitable approximation.
Release of 51Cr-endotoxin from bacteria as an assay of serum bactericidal activity.

In order to facilitate the study of normal serum on Gram-negative bacteria we have developed a bactericidal assay based on the release of 51Cr from labeled bacteria. Release of radioactivity was shown to be mediated by complement and paralleled the bactericidal action of sera measured by change in colony-forming units. Serum from the C6 deficient rabbits and serum that had been treated to inactivate complement did not release 51Cr. The rate of release of 51 Cr was decreased by absorption of serum with Bentonite but the maximum percentage released was not decreased. 51Cr was not released from serum resistant bacteria. The 51Cr appears to be associated with the lipopolysaccharide fraction of the bacteria. Since this structure is the target for the bactericidal reaction, the release of 51Cr is a direct measure of the effect of complement on the bacteria. Under most conditions this reaction leads to cell death so that the release of 51Cr can be used as an assay of bactericidal activity. It was shown that the assay was feasible with Escherichia coli, Salmonella and Hemophilus influenza B.
The interleukin-1 receptor antagonist can either reduce or enhance the lethality of Klebsiella pneumoniae sepsis in newborn rats

Klebsiella pneumoniae, a worldwide cause of nosocomial infections, is one of the most common causes of death in newborns in nurseries. In this study, we investigated the role of interleukin-1 (IL-1) in an experimental animal model of neonatal sepsis, using a natural antagonist of IL-1 receptors, the IL-1 receptor antagonist (IL-1Ra), to block IL-1's effects in neonatal Klebsiella sepsis in the absence of antibiotic treatment. Newborn Wistar-Kyoto rats were injected intraperitoneally with a single dose (10 mg/kg) of either IL-1Ra (n = 43) or human serum albumin as a control (n = 40). At the same time, a 50% lethal dose of K. pneumoniae was injected subcutaneously. No antibiotics were given at any time. After 10 days, survival was 60% for the albumin group and 80% for the IL-1Ra group (P < 0.01). IL-1Ra treatment also afforded protection when the dose of bacteria was increased sixfold (P < 0.01). There were two episodes of leukopenia in the control group, which were suppressed by IL-1Ra (P < 0.01 and P < 0.001). IL-1 and IL-6 levels were lower in the IL-1Ra-treated group (P < 0.05 and P < 0.001, respectively). No differences between the two groups were observed in the number of bacteria in cultures of the blood, lungs, liver, or spleen. When IL-1Ra (10 mg/kg) was given both at time zero and 24 h after bacterial challenge, lethality was significantly increased (P < 0.01). Single doses of IL-1Ra of from 20 to 40 mg/kg progressively increased lethality compared with controls (P < 0.01) in both Wistar-Kyoto and Sprague-Dawley strain rats. In the same model, low doses of IL-1 itself (0.4 ng per rat), given 24 h prior to bacterial challenge, afforded protection (P < 0.001). These studies suggest that, in the absence of antibiotics, partial blockade of IL-1 receptors improves survival, whereas a longer or greater blockade increases lethality in newborn rats infected with K. pneumoniae.
Distributed beamforming for SINR balancing approach in cognitive two-way relay networks with imperfect channel state information

A cooperative bidirectional relaying scheme based on distributed relay beamforming is proposed for an underlay cognitive radio network which consists of two secondary transceivers and K cognitive relay nodes and a primary network with a transmitter and receiver, all equipped with single antenna. For this network we assume that the second-order statistics of the channel coefficients between the relays and primary network is available. Also the perfect channel state information (CSI) is assumed for secondary network. Here, we propose a signal-to-interference plus noise-ratio (SINR) Balancing based on distributed relay beamforming. Our aim is to design the beamforming coefficients to maximize quality of service (QoS) for the secondary network while satisfying tolerable interference constraint for the primary network. Using semidefinite relaxation we turned this problem into convex feasibility problem which can be solved along with bisection search method. Our simulation results show that the uncertainty about the channel coefficients can be degrade the performance of the network considerably. Also as we increased the tolerable interference on primary receiver, the maximum achievable SINR is increased.
Nuclear and cytoplasmic distribution of cellular myb protein in human haematopoietic cells evidenced by monoclonal antibody.

A monoclonal antibody was used for analysing the expression of the cellular myb (c-myb) protein in a variety of established human tumor cell lines and its decrease after induction of differentiation. Differentiated resting human T-cells and B-cells do not express detectable amounts of c-myb protein. However, upon mitogenic stimulation in vitro T-cells exhibit strong expression of the c-myb protein as demonstrated by immunocytochemical staining and indirect immunoprecipitation. In contrast to the transformed T-lymphoblastic cell line Molt-4, where c-myb protein is a nuclear antigen, it was found in proliferating normal T-cells almost exclusively distributed in the cytoplasm. Screening of a total of 70 fresh human malignant lymphomas by immunohistochemical staining indicates the presence of the c-myb protein primarily in non-Hodgkin's lymphomas with a large growth fraction, i.e. precursor cell-derived lymphoblastic lymphomas of B-cell type and T-cell type (9/10, 3/4, respectively) and anaplastic large cell Ki-1 lymphomas (5/9), which originate from activated lymphoid cells. The c-myb protein was located predominantly in the nucleus and in some cases additionally in the cytoplasm. The different subcellular locations suggest a dual functional role. While nuclear localisation is exhibited by transformed haematopoietic cells, cytoplasmic localisation appears to be characteristic for proliferating normal T-cells and points to a second property of the c-myb protein other than interaction with DNA.
Spontaneous Low-Frequency Oscillations Decline in the Aging Brain

It is well known that aging leads to a degeneration of the vascular system. Hence, one may hypothesize that spontaneous oscillations decrease in the cerebral microvasculature with aging. Accordingly, the authors investigated the age dependency of spontaneous oscillations in the visual cortex during rest and functional activation. Functional near-infrared spectroscopy was used because it is particularly sensitive to the microvasculature. Visual stimulation led to an increase of oxyhemoglobin, total hemoglobin, and a decrease of deoxyhemoglobin, without any influence of age. Peaks of normalized power spectral density were detected for spontaneous low-frequency (0.07 to 0.11 Hz) and very-low-frequency (0.01 to 0.05 Hz) oscillations, with a higher amplitude for oxyhemoglobin than for deoxyhemoglobin. Spontaneous low-frequency oscillations of oxyhemoglobin and deoxyhemoglobin declined strongly with aging during both rest and visual stimulation. Reduction of spontaneous low-frequency oscillations might indicate a declining spontaneous activity in microvascular smooth muscle cells, in conjunction with an increased vessel stiffness with aging.