Miracle-dz/UniFAR-FTUs huggingface dataset:
Mécanismes du transfert intercellulaire des homéoprotéines

Les homeoproteines constituent une famille de facteurs de transcription dont le point commun est la presence en leur sein d’un domaine de liaison a l’ADN particulier, l’homeodomaine. Des travaux menes au laboratoire ont permis de mettre en avant la capacite de nombreux membres de cette famille proteique a pouvoir transferer entre cellules, ouvrant la voie a un nouveau mode d’action de ces proteines, en plus de leur action sur la transcription. L’homeodomaine joue un role central dans ce transfert intercellulaire car il contient des sequences requises pour les deux etapes principales d’un tel transfert intercellulaire, secretion et internalisation. D’un point de vue mecaniste, ces deux etapes successives du transfert intercellulaire des homeoproteines font appel a des mecanismes designes comme non-conventionnels car propres a ce processus. Suite a de precedentes etudes menees dans le laboratoire mettant en evidence un role crucial de la localisation subcellulaire de l’homeoproteine ENGRAILED-2 (EN-2) dans sa secretion, nous avons pu mettre en avant l’existence d’une interaction directe entre EN-2 et certains lipides appartenant a la classe des phosphoinositides [notamment les PI(4,5)P2]. Nous avons ensuite montre que cette interaction est a l’origine de l’association d’une fraction de EN-2 intracellulaire avec les compartiments membranaires. Ces travaux ont egalement permis de mieux comprendre le role de certains proteoglycanes dans l’accumulation de EN-2 a la surface des cellules suite a sa secretion et au cours de son internalisation. Enfin nous avons pu mettre en evidence l’implication du domaine d’interaction de EN-2 avec PBX dans le transfert intercellulaire de EN-2.
Development of a return-to-work planning tool for cancer survivors

Background. The ability to return to work (RTW) after a cancer diagnosis can present significant challenges for survivors and can be an important predictor of their long-term quality of life. Survivors report concerns related to disclosing their cancer diagnosis, describing ongoing late effects of cancer, and negotiating workplace accommodations. Purpose. This paper outlines the development of an RTW planning template (RTW-PT) designed to improve communication with stakeholders involved in the RTW process. Lessons learned throughout the process of developing the RTW-PT and implications for clinical practice are presented. Key Issues. The RTW-PT assists the survivor and his or her health care provider to prioritize job demands during a graded RTW and to identify potential accommodations. The RTW-PT also helps survivors plan how they will communicate their RTW needs, particularly with employers and insurance representatives. Implications. The RTW-PT offers a structured approach to support communication among stakeholders involved in the RTW process and to assist survivors in negotiating workplace accommodations.
Dual Substrate Specificity of an N-Acetylglucosamine Phosphotransferase System in Clostridium beijerinckii

ABSTRACT The solventogenic clostridia have a considerable capacity to ferment carbohydrate substrates with the production of acetone and butanol, making them attractive organisms for the conversion of waste materials to valuable products. In common with other anaerobes, the clostridia show a marked dependence on the phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) to accumulate sugars and sugar derivatives. In this study, we demonstrate that extracts of Clostridium beijerinckii grown on N-acetylglucosamine (GlcNAc) exhibit PTS activity for the amino sugar. The PTS encoded by the divergent genes cbe4532 (encoding the IIC and IIB domains) and cbe4533 (encoding a IIA domain) was shown to transport and phosphorylate GlcNAc and also glucose. When the genes were recombined in series under the control of the lac promoter in pUC18 and transformed into a phosphotransferase mutant (nagE) of Escherichia coli lacking GlcNAc PTS activity, the ability to take up and ferment GlcNAc was restored, and extracts of the transformant showed PEP-dependent phosphorylation of GlcNAc. The gene products also complemented an E. coli mutant lacking glucose PTS activity but were unable to complement the same strain for PTS-dependent mannose utilization. Both GlcNAc and glucose induced the expression of cbe4532 and cbe4533 in C. beijerinckii, and consistent with this observation, extracts of cells grown on glucose exhibited PTS activity for GlcNAc, and glucose did not strongly repress utilization of GlcNAc by growing cells. On the basis of the phylogeny and function of the encoded PTS, we propose that the genes cbe4532 and cbe4533 should be designated nagE and nagF, respectively.
Condition Monitoring IGBT Module Bond Wires Fatigue Using Short-Circuit Current Identification

Finding and replacing the defective insulated-gate bipolar transistor (IGBT) module timely by monitoring the ageing state of IGBT can improve the reliability of a power converter and reduce the loss caused by IGBT failure. A method for detecting the condition of bond wires in IGBT module by identifying the short-circuit current of IGBT module is presented in this paper. It is based on the fact that parasitic parameters of IGBT module are affected by the local damage induced by ageing over time, and these changes can be easily detected by monitoring the difference of short-circuit current. The study results indicate that short-circuit current of IGBT module decreases with module ageing over time, and the difference of short-circuit current caused by junction temperature is much smaller than that caused by bond wires fatigue on a specific gate driving voltage. Therefore, the IGBT module in the power converter can be diagnosed by detecting the difference of short-circuit current without considering the effects of junction temperature. Finally, a confirmatory experiment is carried out, and the correctness of the method proposed in this paper is verified.
G4: a fault-tolerant CMOS mainframe

G4 is IBM's fourth generation CMOS microprocessor-based S/390 mainframe but the first to achieve fault tolerant equivalence-or superiority-with its predecessor ECL mainframes. CMOS technology provides much greater density and integration, assuring superior fault avoidance characteristics. The reduced power of CMOS makes bulk power redundancy and battery backup practical. However, the high density and circuit properties of CMOS pose new challenges for detection, recovery, and online repair. G4 implements an innovative design for a high performance, fault tolerant, single-chip microprocessor. Microprocessor sparing is used as a concurrent repair mechanism. Increased memory density requires new (76,64) S4EC/DED Error Correction Codes so that all single chip failures are correctable. As many as four I/O interfaces are packaged on an individual card, requiring both configuration management and automated maintenance procedures to assure all devices maintain connectivity during online repair.
On-Line Handy Handwriting Chinese Characters Input for Non-Chinese Speakers Based on Wavelet Neural Network

Non-Chinese speakers hold increasing opportunities and need to process Chinese information and communicate in Chinese. This paper, with the purpose of facilitating the handwriting input of Chinese characters for non-Chinese speakers, is directed towards the development of the handwriting rules and vocabulary for Latin-style anti-cursive characters and the ways of their selection and classification. This aims to build a practical platform by utilizing three characteristics of wavelet neural network — automatically ascertaining the number of hidden layer unit, converging rapidly and never running into the partial minimum of networks — for a simple Latin-style online handwriting input and processing, meanwhile, taking the customary handwriting habits of non-Chinese speakers. The paper, based on profound information of cursive characters, deciphered the genetic code of ancient cursive symbols and made clear the rules for characters changing into its cursive style. As a result, it breaks the bottleneck, which .
Iron Humate as a Low-Cost Sorbent for Metal Ions

Iron humate produced as a waste by-product during an industrial manufacture of humic substances from low-rank brown coals was tested as a sorbent for the removal of metal cations – Cd (II), Cu (II), Co (II), Ni (II), Zn (II), Tl (I), Eu (III), Cr (III) - as well as hexavalent chromium from waters. The Langmuir-type isotherms were used to describe the metal sorption; the respective equations may be derived and interpreted on the basis of a concept of surface–complexation reactions. Parameters of the sorption isotherms were estimated from experimental dependencies measured in a batch arrangement. The sorption capacities ranged from 0.024 mmol g−1 for Co (II) to 0.324 mmol g−1 for Tl (I). The metal uptake was affected by the presence of complexing agents (EDTA, salicylate, citrate), but this effect depends strongly on pH and the kind of complexing agent. The sorption of Cu (II) ions was suppressed in the presence of EDTA in an almost whole examined pH range (ca. 1 – 6.5) and in the presence of citrate at higher pH values (above pH 4). In the presence of salicylate, on the other hand, a slight sorption enhancement was observed. The metal sorption was suppressed also in the presence of anionic surfactant (sodium dodecylsulfate). Both in the presence as well as in the absence of complexing agents, the metal sorption showed a strong dependence on pH – the sorption was reduced considerably at low pH values below ca. 2. A typical working range for iron humate as a sorbent is ca. pH 3 – 5, where it exhibits a high buffering capacity, a sufficient stability and metal-binding capability.
Peripheral SLC6A4 Gene Expression in Obsessive-Compulsive Disorder in the Han Chinese Population

Background Serotonergic system dysfunction has been implicated in obsessive-compulsive disorder (OCD). This study examined peripheral SLC6A4 gene expression in OCD patients and healthy controls to explore the relationship between SLC6A4 and OCD. Methods Participants included 50 first episode OCD patients and 60 age and gender-matched healthy controls. Relative SLC6A4 gene expression were examined by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) in peripheral leukocytes of all the subjects. The Yale-Brown Obsessive Compulsive Scale (Y-BOCS) was used to assess the severity and subtype of OCD. Results SLC6A4 gene expression, normalized by glyceraldehyde-3-phosphate dehydrogenase (GAPDH), were not significantly different between the OCD patients and healthy controls(z=-0.79, p=0.428). Male OCD patients showed a tendency of low gene expression of SLC6A4 in peripheral blood (z=-1.66, p=0.096). We did not find a significant correlation between SLC6A4 expression and the severity and subtype of OCD. Conclusion There is no correlation between SLC6A4 expression levels and the severity and subtype of OCD, but male OCD patients showed a tendency of low gene expression of SLC6A4 in peripheral blood. These results suggest that gene expression of SLC6A4 in peripheral blood may not be a useful biomarker of OCD in the Han Chinese population.
Comparison of Antimicrobial Susceptibility of Pharyngeal , Rectal , and Urethral Neisseria gonorrhoeae 1 Isolates Among Men Who Have Sex with Men

1 Comparison of Antimicrobial Susceptibility of Pharyngeal, Rectal, and Urethral Neisseria gonorrhoeae 1 Isolates Among Men Who Have Sex with Men 2 3 4 Sarah Kidd 1# , Akbar Zaidi 1 , Lenore Asbel 2 , Tamara Baldwin 3 , Beau Gratzer 4 , Sarah Guerry 5 , Roxanne P. 5 Kerani 6 , Preeti Pathela 7 , Kevin Pettus 1 , Olusegun O. Soge 8 , Ali Stirland 5 , Hillard S. Weinstock 1 6 7 8 1 Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA 9 10 2 Philadelphia Department of Public Health, Philadelphia, PA, USA 11 12 3 Laboratory Services Section, Texas Department of State Health Services, Austin, TX, USA 13 14 4 Howard Brown Health Center, Chicago, IL, USA 15 16 5 Los Angeles County Department of Public Health, Los Angeles, CA, USA 17 18 6 University of Washington and Public Health—Seattle and King County, Seattle, WA, USA 19 20 7 New York City Department of Health and Mental Hygiene, New York City, NY, USA 21 22 8 Neisseria Reference Laboratory, University of Washington, Seattle, WA, USA 23 24 25 #Corresponding Author: 26 Sarah Kidd 27 Division of STD Prevention 28 Centers for Disease Control and Prevention 29 1600 Clifton Road NE, MS E-02 30 Atlanta, GA 30333 USA 31 Email: skidd@cdc.gov 32 Phone: 404-639-8314 33 Fax: 404-639-8610 34 35
Research on the dynamic latent factor model combining attributes of user and item

Cold-start problem is a key challenge in recommender system. In order to solve this problem, we proposed a dynamic latent factor model that combined with attributes of user and item. With the increase of interaction between user and recommender system, our model can automatically adjust its coefficient to achieve a more reasonable and more satisfactory recommendation. The experimental results show that the proposed method can not only alleviate the cold-start problem of new users and new items, but also improve the recommendation accuracy to some extent.
Low cost transformer isolated boost half-bridge micro-inverter for single-phase grid-connected photovoltaic system

This paper presents a low cost high efficiency transformer isolated micro-inverter for single-phase grid-connected photovoltaic (PV) system. The proposed micro-inverter is composed of two stages, an isolated dc-dc converter stage and an inverter stage with a dc link. A high frequency transformer isolated high voltage gain boost half-bridge dc-dc converter is used at the first stage to achieve maximum power point tracking (MPPT) and to step up the PV voltage to the high voltage dc-link. A pulse width modulated (PWM) full-bridge inverter with LCL filter is used at the second stage to output the synchronized sinusoid current with unity power factor to the grid. By utilizing the transformer leakage inductance, two primary side switches can achieve zero voltage switching (ZVS). A 210 W prototype of the proposed micro-inverter has been built and tested. The efficiency of the proposed boost half-bridge dc-dc converter has been measured according to the PV curve, which is up to 98.2%. Experimental results are provided to demonstrate the validity and features of the proposed circuit.
Biomphalaria straminea (Mollusca: Planorbidae) as an Intermediate Host of Ribeiroia sp. (Trematoda: Psilostomidae) in Brazil

Abstract: Species of Ribeiroia are trematode parasites of birds and mammals that have acquired notoriety since Ribeiroia ondatrae was identified as a cause of mortality and malformations in North American amphibians. Although species of Ribeiroia have been reported in vertebrate hosts in South America, the snails involved in its transmission remain unknown in Brazil. During malacological studies conducted at Pampulha Reservoir, Belo Horizonte, Minas Gerais, Brazil, between January 2009 and February 2012, in total 14,264 specimens of Biomphalaria straminea were collected, of which 192 (1.35%) were infected with gymnocephalous cercariae. The larvae were used for experimental infection of laboratory-reared guppies (Poecilia reticulata); metacercariae obtained in these fishes were orally administered to domestic ducks (Cairina moschata); and adult parasites were obtained from the proventriculus 10 days after infection. Based on morphological and molecular analyses, the parasite was identified as Ribeiroia sp. , a species morphologically similar to R. ondatrae, but distinctly different at the molecular level. This is the first report of larvae of Ribeiroia in Brazil and B. straminea as a new intermediate host for this genus.
Human Bone Collagen Synthesis Is a Rapid, Nutritionally Modulated Process

We developed a direct assay of human bone collagen synthesis using [13C] or [15N] proline and applied it to determine the effects of feeding in young healthy men. Surprisingly, postabsorptive bone collagen synthesis is not sluggish, being ∼0.07%/h more rapid than that of muscle protein, and capable of being stimulated within 4 h of intravenous feeding by 66 ± 13%.
Molecular genetic approaches for the study of virulence in both pathogenic bacteria and fungi.

An understanding of the mechanisms of bacterial and fungal pathogenesis is dependent on the identification and characterization of the microbial gene products that influence the progression of infection. The complexity of host-pathogen interactions, particularly in the context of changing cellular and tissue environments encountered by invading pathogens, has made this task very difficult to approach by physiological or biochemical experimentation. A much more productive approach has been to use genetics and molecular biology. With the development over the past two decades of a variety of molecular genetic screening and selection methods, it is now possible to identify and determine the functions of the major virulence determinants of many pathogens. This is clearly an important milestone because our understanding of pathogenicity has often lagged behind knowledge of host responses to infection, particularly mammalian immunity. A detailed understanding of the sequence of events in pathogenesis can also be expected to result in a more informed approach to the development of new antibacterial and antifungal drugs, the need for which has been brought into sharp focus with the emergence of multipledrug-resistant bacteria and the rising numbers of immunosuppressed patients who are often at risk of opportunistic infections which are difficult to treat (Cohen, 1996).
Detection of Known and Unknown Intrusive Sensor Behavior in Critical Applications

This article presents a hybrid architecture to identify intrusive behavior among networked sensors that monitor critical systems such as environment, medical, and smart grid. Monitoring through sensors is desired for critical applications such as epilepsy seizures, pollution, power quality assessment, and transformer monitoring. Wireless sensors are being widely used in critical applications due to their advantages including low-cost, flexibility, and communication efficiency. However, when sensors are networked to monitor a critical infrastructure such as the smart grid, they become the target of different types of attackers such as intruders via the communication medium. In order to maintain sensing in a secure manner, robust architectures are needed to identify intrusive behavior of sensors in a network. In this article, we present a hybrid architecture to detect intrusive behavior of sensors for both unknown and known intruders. The former requires anomaly detection, whereas the latter requires signature detection. The proposed architecture consists of two subsystems that co-operate to detect unknown and known attacks through duty-cycling of enhanced density-based spatial clustering of applications with noise and random forest methods. Through various tests on real intrusion data, we show that the proposed architecture has a strong potential to detect both known and unknown intrusive behavior of sensor nodes as the results show 99.73 detection rate with 98.95 overall accuracy.
Optimum step size of sign algorithm for nonstationary adaptive filtering

The adaptive filtering sign algorithm is analyzed in the case of nonstationary and correlated data. The excess time average absolute estimation error (EAAE) is used as a performance index of the algorithm. It is proved that the EAAE is the sum of two terms: one proportional to the algorithm step size mu and the other proportional to 1/ mu . The optimum step size that minimizes the EAAE is found equal to square root Q/P, with Q being the power of fluctuations of the optimal filter weights, and P being the power of filter input. This formula is derived under the assumption that increments of optimal filter weights are white. Computer simulations are used to examine the validity of the formula for other models of variation of the optimal filter. Simulation results show a good fit with the obtained optimum step size formula. >
Measuring visual acuity--mesopic or photopic conditions, and high or low contrast letters?

PURPOSE To develop single-valued wavefront aberration metrics that correlate strongly with visual performance. The purpose of this study is to explore whether photopic high contrast visual acuity (VA) is an appropriate visual performance reference and whether mesopic and/or low contrast testing provides any advantage. METHODS Subjects from the Texas Investigation of Cataract Optics study (N = 148) ranged in age from 21.6 to 83.8 years and from clear lens to dense nuclear cataract. Visual acuity was measured under four conditions: photopic high (VA(PHC)) and low (VA(PLC)) contrast, mesopic high (VA(MHC)) and low (VA(MLC)) contrast (photopic = 280 cd/m(2), mesopic = 0.75 cd/m(2)). Variables were tested for compliance with normality (-2.00 < skew and kurtosis < 2.00) and transformed if required. Linear regression and Bland-Altman 95% limits of agreement (+/-1.96 SD) were used to examine relationships between VA variables and between VA and wavefront aberration metrics. RESULTS The two photopic measures VA(PHC) (skew = 2.57, kurtosis = 12.2) and VA(PLC) (1.67, 5.41) were poorly distributed, but the two mesopic measures VA(MHC) (0.88, 1.67) and VA(MLC) (0.29, -0.18) were normally distributed. Strong correlations existed between the (transformed) VA variables (R: 0.53 to 0.84). However, limits of agreement ranged over 0.30 to 0.42 logMAR, whereas retest data suggested a range of 0.15. All four were offered in stepwise multiple linear regression for 30 wavefront metrics: 20 included VA(MLC) alone, two included VA(PLC), two included VA(MHC) and six included both VA(MLC) and VA(MHC); R(2) averaged 25%. CONCLUSION Although strongly correlated, low contrast and/or mesopic VA testing provides different information. Wave aberration metrics correlates better with VA(MLC) making this the visual performance test of choice.