Miracle-dz/UniFAR-FTUs huggingface dataset:
Narrowing Down the Mapping of Plant Sex-Determination Regions Using New Y-Chromosome-Specific Markers and Heavy-Ion Beam Irradiation-Induced Y-Deletion Mutants in Silene latifolia

Silene latifolia is a well-studied model system for plant XY sex determination. Three maleness factors are thought to function on the Y chromosome, gynoecium suppression factor (GSF), stamen-promoting factor (SPF), and male fertility factor (MFF), and their deletions result in hermaphrodites, anther defects, and pollen defects, respectively. Although a framework map of the Y chromosome exists, the sex determination genes have not been identified, and no markers close enough to potentially be used for BAC library screening are yet available. The analysis of Y deletion mutants by Y-chromosome-specific STS markers is an efficient way to isolate sex determination regions, but more Y-specific STS markers are needed to accelerate the exploration of sex determination factors. Herein, we report a marker design method that uses simple sequence repeats, which is especially effective on the Y chromosome of S. latifolia because it contains many simple sequence repeats. Six new Y-chromosome-specific STS markers were obtained, SmicSy1–6. These were used to detect relatively small Y deletion sites in heavy-ion beam irradiation-induced mutants. The mapping of male sex determination regions was narrowed down by using more markers and smaller-sized Y deletion mutants. One new marker, SmicSy6, is a proximal marker to SPF and, thus, a second index for SPF. The region including SPF is thought to be located between two SPF proximal markers. The flower phenotype correlates with the deletion size of SPF using SPF proximal markers. These findings represent new progress in isolating the sex determination factor, which has been studied for more than 50 years.
Health‐related quality of life of pre‐school children with wheezing illness

Pre‐school children are frequently affected by wheezing illness, with substantial influences on their health‐related quality of life (HRQL). The Infant/Toddler Quality of Life Questionnaire (ITQOL) is the only generic health status measure for children aged 2 months up to 5 years. In this present study we evaluated the impact of wheezing illness in pre‐school children on the HRQL, using the ITQOL. A questionnaire including the ITQOL and ISAAC questions on frequency and severity of respiratory complaints were sent to parents of patients aged 6 months–5 years visiting the outpatient department with wheezing illness. Scale scores of ITQOL of the included children were compared with general population scores. Using multivariate analysis, the influence of general and clinical characteristics on ITQOL scale scores was evaluated. Results are based on 138 children, 59% male, mean age 34 months. Children with wheezing illness scored differently to the general population sample on 8 of the 11 ITQOL scales. Age, comorbidity and employment of the respondent affected scales on child's physical and emotional functioning and parental functioning. Severity of dyspnoea and wheezing, presence of cough, corticosteroid use, and number of GP visits negatively affected scales on the child's physical and emotional functioning. In conclusion, the HRQL as measured by the ITQOL was lower in a group of Dutch pre‐school children with wheezing illness compared to a general population sample. The scale scores were sensitive to age, co morbidity, socio‐economic situation, and disease severity. Pediatr Pulmonol. 2006, 41:993–1000. © 2006 Wiley‐Liss, Inc.
A Feasibility Study of a Culturally Tailored Diabetes Intervention for Mexican Americans

Latinos, the fastest growing minority group in the United States, are among the hardest hit by diabetes. Among Latinos, Mexican Americans have the highest rate (23.9%) of diabetes. Good self-management can improve glycemic control and decrease diabetes complications but can be challenging to achieve. The purpose of this study was to test the feasibility and examine the effects of a culturally tailored intervention for Mexican Americans with type 2 diabetes on outcomes of self-management. The study used a pretest/posttest control group design with 10 participants in each group (N = 17). Feasibility and acceptability of the tailored diabetes self-management program was assessed by examining ease of recruitment and retention rates. The behavioral outcomes of self-efficacy, diabetes knowledge and self-care measures, and the biologic outcomes of weight, body mass index, HbA1C, and blood glucose were used to examine intervention effectiveness. Successful recruitment of participants came from personal referrals from providers or the promotora. Retention rates were 100% for the intervention group and 80% for the control group. Findings suggest that the intervention had a positive clinical and statistical effect on diabetes knowledge, weight, and body mass index. Improvements were also noted in self-efficacy scores, blood glucose, and HbA1C, but these changes did not reach statistical significance. A culturally tailored diabetes self-management program may result in improved outcomes for Mexican Americans with type 2 diabetes.
A national hero or a Wily Politician? Students’ ideas about the origins of the nation in Sweden

ABSTRACT The topic of this article is how primary school students express ideas about the ‘origins of the nation’. The study is based on texts written by Swedish students aged 12–13 about a historical event well embedded in Swedish national mythology, the rise to power of Swedish ‘founding father’ Gustav Vasa. The analysis is inspired by James V. Wertsch's concept of schematic narrative templates. The main finding of the study is that the students mainly structure their answers along one of two different narrative templates; either a kind of historical master narrative with strong foundations in traditional Swedish history culture, or a more generic story about the founding father as a ruthless or corrupt politician, which is arguably influenced by presentism.
Exploiting flow to control the in vitro spatiotemporal distribution of microbubble-seeded acoustic cavitation activity in ultrasound therapy

Focused ultrasound and microbubbles have been extensively used to generate therapeutic bioeffects. Despite encouraging in vivo results, there remains poor control of the magnitude and spatial distribution of these bioeffects due to the limited ability of conventional pulse shapes and sequences to control cavitation dynamics. Thus current techniques are restricted by an efficacy-safety trade-off. The primary aim of the present study was to incorporate the presence of flow in the design of new short pulse sequences, which can more uniformly distribute the cavitation activity. Microbubbles flowing (fluid velocity: 10 mm s−1) through a 300 μm tube were sonicated with a focused 0.5 MHz transducer while acoustic emissions were captured with an inserted focused 7.5 MHz passive cavitation detector. The two foci were co-axially aligned and their focal points were overlapped. Whereas conventional sequences are composed of a long burst (>10 000 cycles) emitted at a low burst repetition frequency (<10 Hz), we decomposed this burst into short pulses by adding intervals to facilitate inter-pulse microbubble movement. To evaluate how this sequence influenced cavitation distribution, we emitted short pulses (peak-rarefactional pressure (PRP): 40–366 kPa, pulse length (PL): 5–25 cycles) at high pulse repetition frequencies (PRF: 0.625–10 kHz) for a burst length of 100 ms. Increased cavitation persistence, implied by the duration of the microbubble acoustic emissions, was a measure of improved distribution due to the presence of flow. Sonication at lower acoustic pressures, longer pulse intervals and lower PLs improved the spatial distribution of cavitation. Furthermore, spectral analysis of the microbubble emissions revealed that the improvement at low pressures is due to persisting stable cavitation. In conclusion, new short-pulse sequences were shown to improve spatiotemporal control of acoustic cavitation dynamics during physiologically relevant flow. This could lead to adjustable distribution of the generated in vivo bioeffect and therefore efficient and safe treatment of a wide range of pathologies.
Responsiveness of NEI VFQ-25 to changes in visual acuity in neovascular AMD: validation studies from two phase 3 clinical trials.

PURPOSE To examine the responsiveness of the National Eye Institute Visual Function Questionnaire-25 (NEI VFQ-25) by using data from the MARINA and ANCHOR trials in neovascular age-related macular degeneration (AMD) and to establish the change in the NEI VFQ-25 associated with a 15-letter change in best corrected visual acuity (BCVA). METHODS In MARINA, 716 patients were randomized to monthly intravitreal ranibizumab (0.3 or 0.5 mg) or sham injections. In ANCHOR, 423 patients were randomized to monthly ranibizumab (0.3 or 0.5 mg) with sham photodynamic therapy (PDT) or sham ocular injections with verteporfin PDT. Patients had follow-up interviews and BCVA measurements over 24 months. Data were analyzed separately for MARINA and ANCHOR, and treatment groups were pooled within each trial. The clinically relevant difference in NEI VFQ-25 was estimated based on regression models of change from baseline to month 12 in BCVA. RESULTS Subgroups categorized by BCVA change (>or=15 letters gained, <15 letters lost or gained, or >or=15 letters lost) differed substantially in mean change in NEI VFQ-25 composite scores and three pre-specified subscale scores (near activities, distance activities, and vision-specific dependency) over 12 months. According to the regression models, the difference associated with a 15-letter change was 4 to 6 points for the composite score and the three pre-specified subscales. CONCLUSIONS These data support the use of the NEI VFQ-25 as a responsive and sensitive measure of vision-related function in neovascular AMD populations. Based on MARINA and ANCHOR data, a 4- to 6-point change in NEI VFQ-25 scores represents a clinically meaningful change corresponding to a 15-letter change in BCVA.
On Instant Knowledge Evolution from Learning User Search Intent

In this paper, we explore a novel problem to identify timely new knowledge triples. In the literature, the need of knowledge enrichment has been recognized as the key to the success of semantic search. However, previous work of automatic knowledge extraction, such as Google Knowledge Vault, aim at identifying the unannotated knowledge triples from the full web-scale content in the offline execution. In our study, we show that most people demand the updated knowledge soon after the information is announced. However, the number of queries of such knowledge dramatically declines after a few days, meaning that the most people cannot obtain the precise knowledge from the execution of the offline knowledge enrichment when they inquire the answer of the timely events. To remedy this, we propose the SCKE framework to extract new knowledge triples which can be executed in the online scenario. We model the 'Query-Click' bipartite graph to extract the query correlation and to identify temporally coexistent entity pairs. Our experimental studies show that new triples can also be identified effectively and efficiently.
Empathy, Imagining and Motivation

Empathy consists of experiencing an emotion as a consequence of observing its occurrence in another person. This conceptualization indicates a much broader range of empathized emotions than the usually researched ones of pain or distress. The process generating empathy entails attention to the other's emotion and imagining what the other is experiencing emotionally. Because people can learn from experiencing empathy and its consequences, they may be motivated to empathize in some situations, to avoid empathizing in others, or to escape from empathy-eliciting situations. A wide range of motivations—prosocial, asocial, or antisocial—may be involved. Throughout, the article presents new hypotheses and areas of research suggested by this approach. We propose that people regulate then-social relationships so as to optimize empathized emotional experiences, on the one hand, and regulate empathic experiences to serve social relationships, on the other.
Seamless Integration of Initial Sketching and Subsequent Detail Editing in Flower Modeling

We present an interactive modeling system for flower composition that supports seamless transformation from an initial sketch to a detailed three‐dimensional (3D) model. To begin, the user quickly sketches the overall appearance of the desired model as a collection of two‐dimensional (2D) strokes on hierarchical billboards. Then the user iteratively replaces the coarse sketch with a detailed 3D model referring to the initial sketch as a guide. Since a flower model consists of many repetitive components, the system helps the user to reuse 3D components to facilitate the modeling process. The global view of the entire model is always shown in a separate window to visualize how local modifications affect the global appearance. Our system helps the user make appropriate design decisions to keep the model consistent with the initial design, which is difficult in traditional bottom‐up plant modeling systems in which the global view only emerges after all of the details are specified.
Fish, n-3 Fatty Acids, and Cardiovascular Diseases in Women of Reproductive Age: A Prospective Study in a Large National Cohort

Previous studies have indicated a protective effect of long-chain n-3 polyunsaturated fatty acids (LCn3FAs) against cardiovascular disease; however, women are underrepresented in cardiovascular research. The aim of this study was to explore the association between intake of LCn3FAs and the risk of cardiovascular disease in a large prospective cohort of young women (mean age at baseline: 29.9 years [range: 15.7–46.9]). Exposure information on 48 627 women from the Danish National Birth Cohort was linked to the Danish National Patients Registry for information on events of hypertensive, cerebrovascular, and ischemic heart disease used to define a combined measure of cardiovascular diseases. Intake of fish and LCn3FAs was assessed by a food-frequency questionnaire and telephone interviews. During follow-up (1996–2008; median: 8 years), 577 events of cardiovascular disease were identified. Low LCn3FA intake was associated with an increased risk of cardiovascular disease (adjusted hazard ratio for women in lowest versus highest LCn3FA intake group: 1.91 [95% CI: 1.26–2.90]). Restricting the sample to women who had consistently reported similar frequencies of fish intake across 3 different dietary assessment occasions tended to strengthen the relationship (hazard ratio for lowest versus highest intake: 2.91 [95% CI: 1.45–5.85]). Furthermore, the observed associations were consistent in supplementary analyses where LCn3FA intake was averaged across the 3 dietary assessment occasions, and the associations were persistent for all 3 of the individual outcomes. Our findings based on a large prospective cohort of relatively young and initially healthy women indicated that little or no intake of fish and LCn3FAs was associated with an increased risk of cardiovascular disease.
Information-Theoretic Analysis of Serial Dependence and Cointegration

This paper is devoted to presenting wider characterizations of memory and cointegration in time series, in terms of information-theoretic statistics such as the entropy and the mutual information between pairs of variables. We suggest a nonparametric and nonlinear methodology for data analysis and for testing the hypotheses of long memory and the existence of a cointegrating relationship in a nonlinear context. This new framework represents a natural extension of the linear-memory concepts based on correlations. Finally, we show that our testing devices seem promising for exploratory analysis with nonlinearly cointegrated time series.
IC-foundry compatible fabrication of low-cost and tiny-sized dog-bone resonant chemical sensors in non-SOI single-wafer

This paper reports a single-side micromachining technology for batch-fabrication of dog-bone resonant gas sensors in single non-SOI wafer. The process has been transferred to the standard IC-foundry company of ASMC, showing advantage in low-cost volume production. The dog-bone structured resonant chemical sensors are designed and fabricated with the novel micro-machining technology. The 0.5mm×0.5mm sized sensor-chip can be high-yield (>93%) fabricated in 6-inch non-SOI wafer to achieve an ultra-low fabrication cost (one US-cent per chip), which supports disposable biochemical applications. Measurement shows that the low-cost sensor has equal resonating performance to its expensive counterpart fabricated from double sides of SOI-wafer. With gas sensitive material loaded, the sensor experimentally exhibits reproducible detection to 1ppm organophosphur VOC vapor and linear output in terms of gas concentration.
Novel aerobic tetracycline resistance gene that chemically modifies tetracycline

A tetracycline resistance gene that was found originally on the Bacteroides plasmid pBF4 confers resistance on Escherichia coli but only when cells are growing aerobically. When E. coli EM24 carrying this aerobic tetracycline resistance (*Tcr) gene is grown in medium containing tetracycline, the resulting spent medium is no longer toxic to tetracycline-sensitive (Tcs) E. coli EM24 (B.S. Speer and A.A. Salyers, J. Bacteriol. 170: 1423-1429, 1988). To determine whether the *Tcr gene product modified tetracycline, we characterized the material resulting from incubation of E. coli (*Tcr) with tetracycline. When [7-3H(N)]tetracycline was added to cultures of E. coli (*Tcr), at least 90% of the label was recovered in the extracellular fluid. Therefore, tetracycline was not being sequestered by the cells. The labeled material behaved similarly to tetracycline with respect to solubility in various organic solvents. However, the UV-visible light spectrum had a single peak at 258 nm, whereas the tetracycline spectrum had a peak at 364 nm. The labeled material also had a faster migration rate than did tetracycline on thin-layer plates in a solvent system of butanol-methanol-10% citric acid (4:1:2, vol/vol/vol) and was separable from tetracycline by reverse-phase high-pressure liquid chromatography, using an acetronitrile-0.1% trifluoroacetic acid solvent system. These results demonstrate that the *Tcr gene product chemically modifies tetracycline. The *Tcr gene is the first example of a chemically modifying tetracycline resistance mechanism.
Particle swarm optimization for voltage stability analysis

An efficient approach to determine the voltage collapse point greatly assists planning and operation of power system, but most of existing methods are engaged with several power flow computations and they do not provide the optimal operating condition, which is the maximum loading point of a system without exceeding the power limits of generators. This paper introduces a novel approach by particle swarm optimization, which directly determines the voltage collapse point without successive power flow computations and furnishes the optimal operating condition as well. The proposed approach has been tested with a 6-bus test system and 182-bus power system of Sri Lanka as well as it is assessed for diverse applications of voltage stability analysis by concerning about single element outage and the role of static var compensators. Further, performance of the proposed approach is evaluated with continuation power flow method and the optimality is verified by eigenvalue analysis.
Identification of genes preferentially methylated in hepatitis C virus‐related hepatocellular carcinoma

Chronic infections by hepatitis B virus (HBV) and hepatitis C virus (HCV) appear to be the most significant causes of hepatocellular carcinoma (HCC). Aberrant promoter methylation is known to be deeply involved in cancer, including in HCC. In this study, we analyzed aberrant promoter methylation by methylated DNA immunoprecipitation‐on‐chip analysis on a genome‐wide scale in six HCCs including three HBV‐related and three HCV‐related HCCs, six matched noncancerous liver tissues, and three normal liver tissues. Candidate genes with promoter methylation were detected more frequently in HCV‐related HCC. Candidate genes methylated preferentially to HBV‐related or HCV‐related HCCs were detected and selected, and methylation levels of the selected genes were validated by quantitative methylation analysis using MALDI‐TOF mass spectrometry using 125 liver tissue samples, including 61 HCCs (28 HBV‐related HCCs and 33 HCV‐related HCCs) and 59 matched noncancerous livers, and five normal livers. Among analyzed genes, preferential methylation in HBV‐related HCC was validated in one gene only. However, 15 genes were found to be methylated preferentially in HCV‐related HCC, which was independent from age. Hierarchical clustering of HCC using these genes stratified HCV‐related HCC as a cluster of frequently methylated samples. The 15 genes included genes inhibitory to cancer‐related signaling such as RAS/RAF/ERK and Wnt/β‐catenin pathways. Methylation of dual specificity phosphatase 4 (DUSP4), cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1), and natriuretic peptide receptor A (NPR1) significantly correlated with recurrence‐free survival. It was indicated that genes methylated preferentially in HCV‐related HCC exist, and that DNA methylation might play an important role in HCV‐related HCC by silencing cancer‐related pathway inhibitors, and might perhaps be useful as a prognostic marker. (Cancer Sci 2010)
Up‐regulation of P‐glycoprotein reduces intracellular accumulation of beta amyloid: investigation of P‐glycoprotein as a novel therapeutic target for Alzheimer's disease

Objectives Several studies have suggested the efflux transporter P‐glycoprotein (P‐gp) to play a role in the etiology of Alzheimer's disease through the clearance of amyloid beta (Aβ) from the brain. In this study, we aimed to investigate the possibility of P‐gp as a potential therapeutic target for Alzheimer's disease by examining the impact of P‐gp up‐regulation on the clearance of Aβ, a neuropathological hallmark of Alzheimer's disease.
A Survival Guide for the Social Scientist

In this article, we provide the nontechnical reader with a fundamental understanding of the components of virtual reality (VR) and a thorough discussion of the role VR has played in social science. First, we provide a brief overview of the hardware and equipment used to create VR and review common elements found within the virtual environment that may be of interest to social scientists, such as virtual humans and interactive, multisensory feedback. Then, we discuss the role of VR in existing social scientific research. Specif- ically, we review the literature on the study of VR as an object, wherein we discuss the effects of the technology on human users; VR as an application, wherein we consider real-world applications in areas such as medicine and education; and VR as a method, wherein we provide a comprehensive outline of studies in which VR technologies are used to study phenomena that have traditionally been studied in physical settings, such as nonverbal behavior and social interaction. We then present a content analysis of the literature, tracking the trends for this research over the last two decades. Finally, we present some possibilities for future research for interested social scientists.