Miracle-dz/UniFAR-FTUs huggingface dataset:
Cycle Length‐Associated Modulation of the Regional Dispersion of Ventricular Repolarization in a Canine Model of Long QT Syndrome

CHINUSHI, M. , et al.: Cycle Length‐Associated Modulation of the Regional Dispersion of Ventricular Repolarization in a Canine Model of Long QT Syndrome. Previous tridimensional activation mapping showed that the development of functional conduction block at the onset of torsades de pointes was regionally heterogeneous; conduction block was frequently observed in the LV and the interventricular septum (IVS) but not in the RV, in the canine anthopleurin‐A (AP‐A) model of long QT syndrome (LQTS). This may be related to the distribution of myocytes with M celllike electrophysiological characteristics. To better understand the regional difference of arrhythmogenicity in LQTS, the authors investigated cycle length related modulation of ventricular repolarization among three different layers: the endocardium (End), mid‐myocardium (Mid), and epicardium (Epi) of the LV and RV and at two different areas: the Epi and septum (Sep) in the IVS. The LQT3 model was produced by AP‐A in dogs. Using constant pacing and single premature stimulation (S1S2), the ventricular repolarization pattern was analyzed from 256 unipo2 lar electrograms. Activation‐recovery intervals (ARIs) were used to estimate local repolarization. In seven experiments, AP‐A increased regional ARI dispersion to 88.1 ± 36.0 ms in the LV, to 72.9 ± 35.7 ms in the IVS, and to 23.0 ± 8.7 ms in the RV at the pacing cycle length (CL) of 1,000 ms. Development of the large ARI dispersion was due to greater ARI prolongation at the Mid site in the LV and at Sep site in the IVS. As the S1S2 interval was shortened, regional ARI dispersion decreased gradually, and finally, ARI dispersion showed a reversal gradient of repolarization between the Mid and Epi sites in the LV and between the Sep and Epi sites in the IVS. Two factors contributed to create the reversal gradient of repolarization: (1) a difference in restitution kinetics at the Mid site in the LV and at the Sep site in the IVS, characterized by a larger Δ ARI and slower time constant (τ), and (2) a difference in diastolic intervals at each site resulting in different input to restitution at the same CL. However, the RV showed small alteration in the transmural dispersion of repolarization in the S1S2 protocol. S2 created heterogeneous functional conduction block in the LV and IVS but not in the RV. In the LQT3 model, the arrhythmogenicity of torsades de pointes is primarily due to dispersion of repolarization in the LV and IVS because of prominent distribution of M cells. The RV seems to participate passively in reentrant excitation during torsades de pointes.
80 GHz low noise amplifiers in 65nm CMOS SOI

A 1 stage and 3 stages 80 GHz low noise amplifiers (LNA) are presented in this paper. Both mm-wave LNA are integrated in a 65 nm CMOS SOI process. The one stage amplifier exhibits 2.1 dB gain and a noise figure of 4.5 dB at 80 GHz. The input and output return losses are -13 dB and -6 dB respectively. This amplifier consumes 22 mW from a supply voltage of 1.2 V and occupies an area of 0.64 mm2 including the pads. The 3 stages LNA presents a gain of 7.2 dB and a noise figure of 5.7 dB at 80 GHz with an input and output matching better than -14 dB and -10 dB respectively. The 3 stages amplifier consumes 70 mW from a supply voltage of 1V and occupies an area of 0.98 mm2 including pads.
Modeling and Optimization of Magnetically Coupled Resonant Wireless Power Transfer System With Varying Spatial Scales

Previous work reveals that the magnetically coupled resonant (MCR) wireless power transfer (WPT) technology is efficient and practical for mid-range wireless energy transmission, able to handle nontrivial amount of power. Due to the variable coupling coefficient under lateral misalignment and angular misalignment between transmitting coils and receiving coils, the output power and transmission efficiency will fluctuate, leading to instability of the system. This paper presented an equivalent analytical model for the MCR WPT system to incorporate spatial misalignments. The mutual inductance formulas were derived when receiving coils are laterally, angularly or generally misaligned from transmitting coils. The relationship among the output power, transmission efficiency, the mutual inductance, and load resistance were analyzed in detail. For the design of the MCR WPT system, it is necessary to seek optimal transmission performance under different applications. To achieve maximum output power and high stability of power transfer in a specific misalignments range, a normalization method based on the obtained analytical model was introduced, providing critical insight into the optimal design of coils. Relative design considerations and optimization procedures were further stated. Experiments had also been carried out to evaluate the accuracy of theoretical analysis and confirm the validity of the proposed optimization method.
New Order on Type 2 Fuzzy Numbers

Since Lotfi A. Zadeh introduced the concept of fuzzy sets in 1965, many authors have devoted their efforts to the study of these new sets, both from a theoretical and applied point of view. Fuzzy sets were later extended in order to get more adequate and flexible models of inference processes, where uncertainty, imprecision or vagueness is present. Type 2 fuzzy sets comprise one of such extensions. In this paper, we introduce and study an extension of the fuzzy numbers (type 1), the type 2 generalized fuzzy numbers and type 2 fuzzy numbers. Moreover, we also define a partial order on these sets, which extends into these sets the usual order on real numbers, which undoubtedly becomes a new option to be taken into account in the existing total preorders for ranking interval type 2 fuzzy numbers, which are a subset of type 2 generalized fuzzy numbers.
Bearing Witness to the Ethics of Practice: Storying Physicians' Medical Mistake Narratives

Medical mistakes are often referred to as the “hidden epidemic” of health because doctors, patients, and hospital administrators remain silent about mistakes. This study relies on in-depth interviews to explore how physicians story their medical mistake experiences. Narrative theory is used to understand how these physicians story the complexity of medical mistakes, highlighting how these physicians bear witness to medical mistakes by sharing and listening to medical mistake narratives. Moreover, this study showcases the implications of how practitioners and scholars bear witness to the oft-times emotional telling and retelling of health narratives.
Self-assembly of tunable protein suprastructures from recombinant oleosin

Using recombinant amphiphilic proteins to self-assemble suprastructures would allow precise control over surfactant chemistry and the facile incorporation of biological functionality. We used cryo-TEM to confirm self-assembled structures from recombinantly produced mutants of the naturally occurring sunflower protein, oleosin. We studied the phase behavior of protein self-assembly as a function of solution ionic strength and protein hydrophilic fraction, observing nanometric fibers, sheets, and vesicles. Vesicle membrane thickness correlated with increasing hydrophilic fraction for a fixed hydrophobic domain length. The existence of a bilayer membrane was corroborated in giant vesicles through the localized encapsulation of hydrophobic Nile red and hydrophilic calcein. Circular dichroism revealed that changes in nanostructural morphology in this family of mutants was unrelated to changes in secondary structure. Ultimately, we envision the use of recombinant techniques to introduce novel functionality into these materials for biological applications.
The reception of Arab women writers in the West

This thesis explores the reception in the Anglophone West of the works of three Arab women writers Nawel el-Saadawi, Hanan al-Shaykh and Sahar Khalifa. The principal methodological tools are drawn from cultural theories of translation, methods that go beyond a narrow technical focus on accuracy and commensurability in translation. The focus is therefore not on textual analysis, but on (1) political events such as the Iranian revolution of 1979, (2) forms of expertise such as academics, critics, reviewers and translators, and (3) ideological trends, primarily Orientalism and feminism, which all affect the success or failure of reception of a translated text in the host culture. The thesis finds that both forms of expertise and political events play important roles in forging a horizon of expectation, primarily among readerships respecting the content of works which are deemed to be interesting. The principal aim of the thesis, however, is to subject the conventional wisdom, which heavily stresses the overwhelming importance of Orientalism in the reception of female Arab writers in the West, to serious scrutiny. To this end, the oeuvre (mostly fiction) of three prominent writers whose reception has been marked by controversies over Orientalism and feminism was chosen. The goal is not to replace the 'Orientalism' thesis with a simplistic feminism thesis: The present argument accepts that Orientalism has played an important role in the reception of the three writers, although in fractured and varied ways. However, I also argue that feminism has played an important and increasing role in literary reception, particularly in the case of Nawal el-Saadawi. The idea is that feminist expectations and concerns, in conjunction with political events, create a 'knowledge vacuum' among readerships which is then filled by particular, relevant texts. In other words, it is inadequate to shoehorn all forms of Western reception into a vague and hydra-like category of Orientalism. However, the argument does not lionize the feminist movement: I show how feminism is marked on the one hand by Orientalism -a standard claim but also how feminism itself is limited by its concern for gender on the one hand, and forins of political conservatism on the other, especially on controversial issues in Middle East politics, such as Israel / Palestine. I show this last point particularly through my exploration of the reception of the work of Sahar Khalifa. In a broader sense, the thesis aims to indicate how cultural interaction between 'West' and 'East' is more complicated than monolithic and essentialist analyses would have us believe. The idea is to bolster readings of Edward Said which do not fall into this trap. Ultimately, such a reading points beyond the notion of nativism on the one side, and Eurocentrism on the other.
Functional and hierarchical interactions among zebrafish vox/vent homeobox genes

The vertebrate Vox/Vent family of transcription factors plays a crucial role in the establishment of the dorsoventral (DV) axis, by repressing organizer genes such as bozozok/dharma, goosecoid, and chordino. In Danio rerio (zebrafish), members of the vox/vent gene family (vox/vega1, vent/vega2, and ved) are thought to share expression patterns and functional properties. Bringing novel insights in the differential activity of the zebrafish vox/vent genes, we propose a critical role for the ved gene in DV patterning of vertebrate embryos. ved is not only expressed as a maternal gene, but it also appears to function as a repressor of dorsal factors involved in organizer formation. At early‐ and mid‐gastrula stage, ved appears to be finely controlled by antagonist crosstalks in a complex regulatory network, involving gradients of bone morphogenetic protein (BMP) activity, dorsal factors, and vox/vent family members. We show that ved transcripts are ventrally restricted by BMP factors such as bmp2b, bmp7, smad5, and alk8, and by dorsal factors (chd and gsc). Alteration of ved expression in both vox and vent deletion mutants and vox and vent mRNAs‐injected embryos, suggests that vox and vent function downstream of BMP signaling to negatively regulate ved expression. This inhibitory role is emphasized by a vox and vent redundant activity, compared with single gene effects. Developmental Dynamics 230:494–508, 2004. © 2004 Wiley‐Liss, Inc.
Alterations of highly conserved residues in the regulatory domain of nitrogen regulator I (NtrC) of Escherichia coli

Transcription of many nitrogen-regulated (Ntr) genes requires the phosphorylated form of nitrogen regulator I (NRI, or NtrC), which binds to sites that are analogous to eukaryotic enhancers. A highly conserved regulatory domain contains the site of phosphorylation and controls the function of NRI. We analyzed the effects of substitutions in highly conserved residues that are part of the active site of phosphorylation of NRI in Escherichia coli. Fourteen substitutions of aspartate 54, the site of phosphorylation, impaired the response to nitrogen deprivation. Only one of these variants, NRI D-54-->E (NRI-D54E), could significantly stimulate transcription from glnAp2, the major promoter of the glnALG operon. Cells with this variant grew with arginine as a nitrogen source. Experiments with purified components showed that unphosphorylated NRI-D54E stimulated transcription. In contrast, substitutions at aspartate 11 were not as deleterious as those at aspartate 54. Finally, we showed that NRI-K103R, in which arginine replaces the absolutely conserved lysine, is functionally active and efficiently phosphorylated. This substitution appears to stabilize the phosphoaspartate of NRI. The differences between our results and those from study of homologous proteins suggest that there may be significant differences in the way highly conserved residues participate in the transition to the activated state.
A Two-Stage SIR Bandpass Filter With an Ultra-Wide Upper Rejection Band

Two-stage stepped-impedance resonator (SIR) filters are designed to have a wide upper stopband. Two transmission zeros are also created near the passband to form a quasi-elliptic response. For a 20-dB rejection level, with properly designed SIR geometry and tapped-line structure, the leading three spurious resonances of the SIR are suppressed by transmission zeros and respective unmatched singly loaded Q (Qsi) values. Measured results illustrate that stopbands up to 8.4 and 5.3 times the design frequency can be achieved for rejection levels of 20 and 30dB, respectively
Adaptive dynamic balance control of humanoid robot based on leg joints

This paper investigates the problems of the dynamic balance and high energy consumption for humanoid robot. Considering the influence on stability caused by the undesired yaw moment, a novel adaptive dynamic balance control based on leg joints is proposed. According to the moment balance conditions and the analysis about the mechanism resulting in yaw moment, the optimal leg joints angles trajectories is proposed to counteract the yaw moment caused by the movement of humanoid robot. Given the optimized leg joints angles motion, an adaptive control system is proposed to track the desired trajectories with model uncertainties and the stability proof is provided. Simulation results validate the proposed method.
Interferon-γ Released by Gluten-Stimulated Celiac Disease-Specific Intestinal T Cells Enhances the Transepithelial Flux of Gluten Peptides

Celiac sprue is a T-cell-mediated enteropathy elicited in genetically susceptible individuals by dietary gluten proteins. To initiate and propagate inflammation, proteolytically resistant gluten peptides must be translocated across the small intestinal epithelium and presented to DQ2-restricted T cells, but the effectors enabling this translocation under normal and inflammatory conditions are not well understood. We demonstrate that a fluorescently labeled antigenic 33-mer gluten peptide is translocated intact across a T84 cultured epithelial cell monolayer and that preincubation of the monolayer with media from gluten-stimulated, celiac patient-derived intestinal T cells enhances the apical-to-basolateral flux of this peptide in a dose-dependent, saturable manner. The permeability-enhancing activity of activated T-cell media is inhibited by blocking antibodies against either interferon-γ or its receptor and is recapitulated using recombinant interferon-γ. At saturating levels of interferon-γ, activated T-cell media does not further increase transepithelial peptide flux, indicating the primacy of interferon-γ as an effector of increased epithelial permeability during inflammation. Reducing the assay temperature to 4°C reverses the effect of interferon-γ but does not reduce basal peptide flux occurring in the absence of interferon-γ, suggesting active transcellular transport of intact peptides is increased during inflammation. A panel of disease-relevant gluten peptides exhibited an inverse correlation between size and transepithelial flux but no apparent sequence constraints. Anti-interferon-γ therapy may mitigate the vicious cycle of gluten-induced interferon-γ secretion and interferon-γ-mediated enhancement of gluten peptide flux but is unlikely to prevent translocation of gluten peptides in the absence of inflammatory conditions.
The methyltransferase Setdb1 is essential for meiosis and mitosis in mouse oocytes and early embryos

Oocytes develop the competence for meiosis and early embryogenesis during their growth. Setdb1 is a histone H3 lysine 9 (H3K9) methyltransferase required for post-implantation development and has been implicated in the transcriptional silencing of genes and endogenous retroviral elements (ERVs). To address its role in oogenesis and pre-implantation development, we conditionally deleted Setdb1 in growing oocytes. Loss of Setdb1 expression greatly impaired meiosis. It delayed meiotic resumption, altered the dynamics of chromatin condensation, and impaired kinetochore-spindle interactions, bipolar spindle organization and chromosome segregation in more mature oocytes. The observed phenotypes related to changes in abundance of specific transcripts in mutant oocytes. Setdb1 maternally deficient embryos arrested during pre-implantation development and showed comparable defects during cell cycle progression and in chromosome segregation. Finally, transcriptional profiling data indicate that Setdb1 downregulates rather than silences expression of ERVK and ERVL-MaLR retrotransposons and associated chimearic transcripts during oogenesis. Our results identify Setdb1 as a newly discovered meiotic and embryonic competence factor safeguarding genome integrity at the onset of life. Highlighted article: The H3K9 methyltransferase Setdb1 is required during mouse oogenesis to control gene expression, restrain expression of endogenous retroviruses and enable successful progression through meiosis and mitosis.
Cyanide production from glycine by a homogenate from a Pseudomonas species

A cell-free preparation with cyanide-producing activity was obtained from a bacterium, strain C, of the genus Pseudomonas. To preserve activity, an oxidizing agent, e.g., phenazine methosulphage (PMS), had to be added to the cell suspension before disruption by sonic treatment. By the procedure described, a total homogenate made from a 15% (wet weight) bacterial suspension in tris(hydroxymethyl)aminomethane-hydrochloride buffer (0.05 M, pH 8.2) and with PMS (0.4mM) exhibited about 8% of the activity obtained from a suspension of untreated bacteria. In the presence of flavine-adenine dinucleotide (0.3 mM) and PMS (0.4mM), the activity was augmented to about 16% of that of the intact cells. By gradient centrifugation the homogenate was separated into three fractions. The main enzyme activity was associated with those fractions which by electron microscopy were found to consist of membranous structures.
Randomized , Double-Blind , Controlled Study

No author associatedwith this paper has disclosed any which may be perceived to have impending conflict wit statements refer to http://dx.doi. org/10.1016/j.arth. 2015.0 Reprint requests: Zhen Hu Wang, MD, Department o Hospital, 071000, No. 991 Baihua Dong Road, Baoding, He 1 These two authors contributed to this work equally. http://dx.doi. org/10.1016/j.arth. 2015.02. 020 0883-5403/© 2015 Published by Elsevier Inc. Please cite this article as: Gong L, et al, Slee Controlled Study, J Arthroplasty (2015), http Article history: Received 7 October 2014 Accepted 18 February 2015 Available online xxxx
Relative Immunogenicity in Mice of Different Regions of the Human IgG

Monoclonal mouse antibodies to human IgG myeloma proteins were produced and characterized by determining their binding to a series of different purified myeloma proteins. Two types of immunization schedules were used. When the same myeloma protein was used for priming and boosting the mouse, all determinants of the molecule were effectively immunogenic. Of the 353 clones originating from these experiments 42% secreted anti‐Fv antibodies, 57% anti‐CH antibodies, and 0.85% anti‐CL, antibodies. In another schedule only the CL and CH regions were the same in priming and boosting; 270 anti‐CH (94%), 18 anti‐CL (16%), and no anti‐Fv hybridomas were found. Our results indicate that a unit mass of the Fv region was 1.3 times more antigenic than a unit mass of the CH region. A unit mass of the CH region was nearly five times more antigenic than a unit mass of the CL domain when the antigenicity of the Fv region had been excluded. When the antigenicity of the Fv region had not been excluded, a unit mass of the CH region was about twenty times more antigenic than a unit mass of the CL domain. The data suggest that an antigenic competition was taking place between different parts of the molecule and that the strongly antigenic region (Fv) was more efficient in competing out the nearest neighbour (CL) than in competing out other parts of the molecule.
Estimation and prediction in linear mixed models with skew‐normal random effects for longitudinal data

This paper extends the classical linear mixed model by considering a multivariate skew‐normal assumption for the distribution of random effects. We present an efficient hybrid ECME‐NR algorithm for the computation of maximum‐likelihood estimates of parameters. A score test statistic for testing the existence of skewness preference among random effects is developed. The technique for the prediction of future responses under this model is also investigated. The methodology is illustrated through an application to Framingham cholesterol data and a simulation study. Copyright © 2007 John Wiley & Sons, Ltd.