Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibition of Transferrin Recycling and Endosome Tubulation by Phospholipase A2 Antagonists*

We report here that a broad spectrum of phospholipase A2 (PLA2) antagonists produce a concentration-dependent, differential block in the endocytic recycling pathway of transferrin (Tf) and Tf receptors (TfRs) but have no acute affect on Tf uptake from the cell surface. At low concentrations of antagonists (∼1 μm), Tf and TfR accumulated in centrally located recycling endosomes, whereas at higher concentrations (∼10 μm), Tf-TfR accumulated in peripheral sorting endosomes. Several independent lines of evidence suggest that this inhibition of recycling may result from the inhibition of tubule formation. First, BFA-stimulated endosome tubule formation was similarly inhibited by PLA2 antagonists. Second, endocytosed tracers were found in larger spherical endosomes in the presence of PLA2 antagonists. And third, endosome tubule formation in a cell-free, cytosol-dependent reconstitution system was equally sensitive PLA2antagonists. These results are consistent with the conclusion that endosome membrane tubules are formed by the action of a cytoplasmic PLA2 and that PLA2-dependent tubules are involved in intracellular recycling of Tf and TfR. When taken together with previous studies on the Golgi complex, these results also indicate that an intracellular PLA2 activity provides a novel molecular mechanism for inducing tubule formation from multiple organelles.
Cross-Domain Academic Paper Recommendation by Semantic Linkage Approach Using Text Analysis and Recurrent Neural Networks

In this digital age, free-flow and exchange of knowledge and information are of paramount importance. This is the prime reason why we decided to tackle cross-domain linkage. Firstly, we build a system which recommends scholarly academic papers based on the content of news article a user is reading using text analysis techniques. We perform a human expert evaluation to test the system for relevance. Our judges show good agreement with a kappa value of 0.869. To improve the quality of recommendations further, we use an RNN-LSTM model trained on Wikipedia to measure document relevance. We reorder a list of academic papers based on their semantic similarity with the input document using our RNN-LSTM model. Our model achieves a slightly better performance than one of the best document embedding techniques doc2vec (paragraph vector). To the best of our knowledge, ours is the first study linking the domains of News Media and Academic landscape, and bridging the knowledge-gap.
A signature of dynamic biogeography: enclaves indicate past species replacement

Understanding how species have replaced each other in the past is important to predicting future species turnover. While past species replacement is difficult to detect after the fact, the process may be inferred from present-day distribution patterns. Species with abutting ranges sometimes show a characteristic distribution pattern, where a section of one species range is enveloped by that of the other. Such an enclave could indicate past species replacement: when a species is partly supplanted by a competitor, but a population endures locally while the invading species moves around and past it, an enclave forms. If the two species hybridize and backcross, the receding species is predicted to leave genetic traces within the expanding one under a scenario of species replacement. By screening dozens of genes in hybridizing crested newts, we uncover genetic remnants of the ancestral species, now inhabiting an enclave, in the range of the surrounding invading species. This independent genetic evidence supports the past distribution dynamics we predicted from the enclave. We suggest that enclaves provide a valuable tool in understanding historical species replacement, which is important because a major conservation concern arising from anthropogenic climate change is increased species replacement in the future.
Design of stabilizing output feedback nonlinear model predictive controllers with an application to DC-DC converters

This paper focuses on the synthesis of nonlinear Model Predictive Controllers that can guarantee robustness with respect to measurement noise. The input-to-state stability framework is employed to analyze the robustness of the resulting Model Predictive Control (MPC) closed-loop system. It is illustrated how the obtained robustness result can be employed to synthesize asymptotically stabilizing observer-based output-feedback nonlinear MPC controllers for a class of nonlinear discrete-time systems. The developed theory is illustrated by applying it to control a Buck-Boost DC-DC converter.
Evidence for bilateral control of skilled movements: ipsilateral skilled forelimb reaching deficits and functional recovery in rats follow motor cortex and lateral frontal cortex lesions

Unilateral damage to cortical areas in the frontal cortex produces sensorimotor deficits on the side contralateral to the lesion. Although there are anecdotal reports of bilateral deficits after stroke in humans and in experimental animals, little is known of the effects of unilateral lesions on the same side of the body. The objective of the present study was to make a systematic examination of the motor skills of the ipsilateral forelimb after frontal cortex lesions to either the motor cortex by devascularization of the surface blood vessels (pial stroke), or to the lateral cortex by electrocoagulation of the distal branches of the middle cerebral artery (MCA stroke). Plastic processes in the intact hemisphere were documented using Golgi–Cox dendritic analysis and by intracortical microstimulation analysis. Although tests of reflexive responses in forelimb placing identified a contralateral motor impairment following both cortical lesions, quantitative and qualitative measures of skilled reaching identified a severe ipsilateral impairment from which recovery was substantial but incomplete. Golgi‐impregnated pyramidal cells in the forelimb area showed an increase in dendritic length and branching. Electrophysiological mapping showed normal size forelimb representations in the lesioned rats relative to control animals. The finding of an enduring ipsilateral impairment in skilled movement is consistent with a large but more anecdotal literature in rats, nonhuman primates and humans, and suggests that plastic changes in the intact hemisphere are related to that hemisphere's contribution to skilled movement.
Analyses of Global Memory Impairments of Different Etiologies a

Chronic global amnesia is a clinically significant deficit in new learning of verbal and nonverbal material irrespective of stimulus modality; the deficit is disproportionate to other cognitive impairment and should be distinguished from the preservation of skill learning and repetition priming effects in global amnesia. '" The purity and severity of the fact-learning impairment may vary from patient to patients6 Between-subject variability in the deficits of amnesic patients is not surprising because of the number of different etiologies of amnesia. Each etiology has its own neuropathological hallmark, and within an etiology the extent of damage to the affected anatomical and chemical systems may vary. ' Etiologies also may differ in the severity of the acute phase of the illness and in the particular brain structures that are compromised, but different etiologies may be associated with common lesion sites. These sources of variation may account for the differences in memory capacities among amnesic patients. There are two possible explanations for such differences: The first is that a single anatomical or chemical system subserves fact-learning capacities, and thus interference with this system results in a single form of amnesia. The amnesia would have a variety of presentations that would reflect the interaction between insults to this memory system and insults outside this memory system; the latter may cause ancillary cognitive and noncognitive deficits that influence memory-test performance. The second possibility is that there exist multiple fact-learning systems, each specialized for different aspects of memory function. In this case, interference with the different anatomical or chemical systems would produce fundamentally different types of amnesia. One approach to evaluating these alternative explanations proceeds by characterizing the precise nature of the behavioral variation among patients whose amnesias stem from different etiologies and from involvement of different brain regions. If global amnesia is a unitary disorder, then any differences in the memory deficits related to lesion site should be either quantitative (i.e., patients would differ primarily in the magnitude of mnemonic deficits rather than in terms of the pattern of deficits) or fully accounted for by the influence of nonmnemonic deficits. Alternatively, if global amnesia comprises different types of memory disorders affecting different neural
Exploring factors affecting the adoption of mobile office in business: an integration of TPB with perceived value

This study develops and empirically tests a research model to comprehensively examine factors influencing the success of the mobile office i.e., adoption in business. In particular, this study proposes an integrated theoretical framework that combines the theory of planned behaviour TPB and the perspective of perceived value. By doing so, it aims to examine not only fundamental determinants of an information system IS adoption proposed by TPB i.e., attitude, subjective norm, and behavioural control but also various psychological and emotional factors that determine users' attitude towards IS through the perspective of perceived value. The results indicate that perceived value, colleague opinion and self-efficacy are critical antecedents that increase users' intention to adopt new technology. Furthermore, perceived usefulness and social image are the salient drivers of mobile office adoption because they increase users' value; on the other hand, performance risk and psychological risk are the salient barriers to adoption because they reduce users' value.
The syndrome of pachygyria, mental retardation, and arachnoid cysts maps to 11p15

The Syndrome of Pachygyria, Mental Retardation, and Arachnoid Cysts Maps to 11p15 Kaya Bilguvar, Ali K. Ozturk, Fatih Bayrakli, Aslan Guzel, Michael L. DiLuna, Yasar Bayri, Mehmet Tatli, Selahaddin Tekes, Zulfikar Arlier, Katsuhito Yasuno, Christopher E. Mason, Richard P. Lifton, Matthew W. State, and Murat Gunel* Department of Neurosurgery, Yale University School of Medicine, New Haven, Connecticut Program on Neurogenetics, Yale University School of Medicine, New Haven, Connecticut Department of Neurosurgery, Dicle University, Diyarbakir, Turkey Department of Medical Biology and Genetics, Dicle University, Diyarbakir, Turkey Department of Genetics, Yale University School of Medicine, New Haven, Connecticut Child Study Center, Yale University School of Medicine, New Haven, Connecticut Department of Internal Medicine, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut Department of Neurobiology, Yale University School of Medicine, New Haven, Connecticut
Comparison of three treatments for bovine endometritis

Three commercial preparations for the treatment of bovine endometritis were compared: an intrauterine infusion of 1500 mg oxtytetracycline hydrochloride solution, an intramuscular injection of 500 μg cloprostenol (a synthetic analogue of prostaglandin F2α), and an intramuscular injection of 3 mg oestradiol benzoate/500.kg estimated bodyweight. A total of 300 cases of endometritis were treated, of which 225 involved first, 67 involved second, and eight involved third or subsequent treatments. The overall success rate of treatment was 68 per cent. Oxytetracycline was successful in 73 per cent of cases, cloprostenol in 67 per cent and oestradiol in 63 per cent of cases. There was no significant difference between the success rates of the treatments, except for cows with mild endometritis in which oxytetracycline was more successful than oestradol (86 v 66 per cent, P<0.05). Mild cases were treated more successfully than moderate cases (78 v 61 per cent, P<0.01), and more successfully than severe cases (78 v 44 per cent, P<0.001). Prostaglandin F2α was more successful if the milk progesterone concentration was >7 ng/ml at the time of treatment (P<0.05). The presence of a smelly discharge at the time of treatment reduced the success rate by 17 per cent (P<0.02). The treatment to conception interval for all successful treatments of endometritis by prostaglandin F2α was 18.1 days shorter than for oestradiol (68.3 v 86.4 days, P<0.02), and the interval for oxytetracycline was 16.2 days shorter than for oestradiol (70.2 v 86.4 days, P<0.05).
An Effective QRS Detection Algorithm for Wearable ECG in Body Area Network

A novel QRS detection algorithm for wearable ECG devices and its FPGA implementation are presented in this paper. The proposed algorithm utilizes the hybrid opening- closing mathematical morphology filtering to suppress the impulsive noise and remove the baseline drift and uses modulus accumulation to enhance the signal. The proposed algorithm achieves an average QRS detection rate of 99.53%, a sensitivity of 99.82% and a positive prediction of 99.71% against the MIT/BIH Arrhythmia Database. It compares favorably to published methods.
Phosphoenolpyruvate synthetase of Escherichia coli. Purification, some properties, and the role of divalent metal ions.

Abstract A procedure for the preparation of highly purified phosphoenolpyruvate synthetase from Escherichia coli is described. The purified enzyme is markedly stabilized against inactivation by high concentrations of sucrose and is susceptible to inactivation by sulfhydryl reagents. The binding of the activating manganous ion to the dephosphoenzyme was investigated by electron spin resonance and proton relaxation rate of water. The proton relaxation rate of water was enhanced 12 times relative to the Mn2+ aquo-ion. Addition of pyruvate decreased the enhancement, indicating the formation of a dephosphoenzyme-manganese-pyruvate complex. On the basis of a molecular weight of 250,000 it was calculated that 3 to 4 moles of Mn2+ were bound per mole of enzyme with Kd ≅ 30 µm. The phosphate content of phosphoenzyme whether prepared from ATP or phosphoenolpyruvate was 1 to 2 moles of phosphate per mole of enzyme. Initial velocity studies of the forward reaction indicate that an irreversible step occurs between the binding of MgATP to the enzyme and the binding of pyruvate. MgATP and pyruvate combine with the dephospho- and the phosphoenzyme form, respectively, to form kinetically active complexes; the Michaelis constants are Kmgatp = 28 µm and Kpyruvate = 20 µm at 5 mm free Mg2+. A requirement for free divalent metal ion (Mg2+ or Mn2+) in addition to that bound to ATP was indicated by the kinetic studies and was implicated in the binding of pyruvate to the enzyme. At high concentrations of free Mg2+ and Mn2+, the reaction is inhibited. Ca2+ is not an activator but inhibits the Mn2+-activated reaction.
SAPTL-based robust sub-threshold adder circuit design

Process variation results in up to an order of magnitude variation in Ion/Ioff ratios, which significantly depresses the yield of the sub-threshold circuits. This paper presents low power sub-threshold adders using sense amplifier-based pass transistor logic (SAPTL). Based on the simulations in 130nm CMOS process, the proposed two-phase synchronous SAPTL adder exhibits stronger robustness to process variations when compared to the ripple carry adder using standard cell circuits. And the adder based on ADSA-SAPTL features much less power consumption than synchronous SAPTL adder.
The place of contract in organizational awareness: deconstructing process, market and connectedness

Throughout modern business history, contract has been used as an organizational technology that holds counterparties in formal or legally binding agreements. The proliferation of contract prompted the emergence of professional contract managers who played an important but relatively peripheral role applying situations awareness to the practice of compliance and business relationship management. As more complex organizational forms emerge (outsourcing, supply chains and enterprise IS), contract has come to be seen as a standard coordination device whose foundational assumptions are taken for granted. In this study, we draw attention to two different ways of designing the organizational technology of contract: a process-oriented approach and a market-based valuation of contract. Both approaches promise to provide management information by offering a form of calculability but we argue distort organizational awareness. Our conclusion is that a more developed notion of organizational awareness is needed supported by an alternative conceptualization of contract as a technology or connectedness.
Three-dimensional structure of tetrahydrodipicolinate N-succinyltransferase.

The conversion of tetrahydrodipicolinate and succinyl-CoA to N-succinyltetrahydrodipicolinate and CoA is catalyzed by tetrahydrodipicolinate N-succinyltransferase and is the committed step in the succinylase pathway by which bacteria synthesize L-lysine and meso-diaminopimelate, a component of peptidoglycan. The X-ray crystal structure of THDP succinyltransferase has been determined to 2.2 A resolution and has been refined to a crystallographic R-factor of 17.0%. The enzyme is trimeric and displays the left-handed parallel beta-helix (L beta H) structural motif encoded by the "hexapeptide repeat" amino acid sequence motif [Raetz, C.R. H. , & Roderick, S.L. (1995) Science 270, 997-1000]. The approximate location of the active site of THDP succinyltransferase is suggested by the proximity of binding sites for two inhibitors: p-(chloromercuri)benzenesulfonic acid and cobalt ion, both of which bind to the L beta H domain.
All Known In Vivo Functions of the Oct-2 Transcription Factor Require the C-Terminal Protein Domain1

Oct-2, a transcription factor expressed in the B lymphocyte lineage and in the developing CNS, functions through of a number of discrete protein domains. These include a DNA-binding POU homeodomain flanked by two transcriptional activation domains. In vitro studies have shown that the C-terminal activation domain, a serine-, threonine- and proline-rich sequence, possesses unique qualities, including the ability to activate transcription from a distance in a B cell-specific manner. In this study, we describe mice in which the endogenous oct-2 gene has been modified through gene targeting to create a mutated allele, oct-2ΔC, which encodes Oct-2 protein isoforms that lack all sequence C-terminal to the DNA-binding domain. Surprisingly, despite the retention of the DNA-binding domain and the glutamine-rich N-terminal activation domain, the truncated protein(s) encoded by the oct-2ΔC allele are unable to rescue any of the previously described defects exhibited by oct-2 null mice. Homozygous oct-2ΔC/ΔC mice die shortly after birth, and B cell maturation, B-1 cell self renewal, serum Ig levels, and B lymphocyte responses to in vitro stimulation are all reduced or absent, to a degree equivalent to that seen in oct-2 null mice. We conclude that the C-terminal activation domain of Oct-2 is required to mediate the unique and indispensable functions of the Oct-2 transcription factor in vivo.
Service based framework for knowledge portals

This paper describes our work on establishing an on-line learning community to facilitate the interactions of the student (teams) in their group projects. It is illustrated with a Portal in the capstone project of our Bachelor of Software Engineering (BSE) course. [2] reported on our Monash University Software Engineering (MUSE) Studio Lab and the innovative software engineering capstone project experience. Our student teams used to store the various artefacts, which were produced as part of the SE Studio (capstone) projects on the servers in the studio lab. However, these SE product and process artefacts were not accessible uniformly through a web browser in a MUSE Portal. The Portal environment is aimed at providing a virtual learning space, knowledge aggregation and user management. In this paper, we provide details of our pedagogical service based framework, which has been designed and built to provide features to Portal users. The framework supports services such as group/team collaboration, task tracking, and project planning.The collaboration and learning services available from our Knowledge Portal is based on the learning activities as defined by [1] Nonaka1994. Nonaka defines: socializing to exchange views and develop trust between team members, dialoguing to share mental models and develop common vocabulary, systemising to combine knowledge by visualising interactions and constructing artifacts and exercising to communicate and reflect on outcomes. We follow a similar approach to enable team members to discuss and reflect on group findings with respect to SE processes & products during the year long SE Studio project, and reflect over individual thesis components.A pedagogical service based framework can service the current pedagogical needs of the SE group projects, other related subjects, and SE Honours research projects and is also extendible with more services on a demand basis. The focus has been on allowing custom subject specific services to be created without having the inherent overheads.A pedagogical framework with service based architecture has been built to provide services to the Portal users. The Portal Environment is the front-end through which services are exposed. Examples of services are: task tracking and project planning in group projects; collaboration between team members in a group project in a subject unit; knowledge repository of product/project assets; calendar facility etc. Support infrastructure services of user authentication, role and service allocation etc. are also managed via the portal environment.We show how on-line learning communities can be supported using the MUSE knowledge portal. The framework supports services such as team collaboration, task tracking, and project planning. The url for MUSE Portal is http://museportal.csse. monash.edu. au:8080/MUSEPortalWeb/.
Elevated Lifetime Lead Exposure Impedes Osteoclast Activity and Produces an Increase in Bone Mass in Adolescent Mice.

The heavy metal lead (Pb) has a deleterious effect on skeletal health. Because bone mass is maintained through a balance of bone formation and resorption, it is important to understand the effect of Pb levels on osteoblastic and osteoclastic activity. Pb exposure is associated with low bone mass in animal models and human populations; however, the correlation between Pb dosing and corresponding bone mass has been poorly explored. Thus, mice were exposed to increasing Pb and at higher levels (500 ppm), there was unexpectedly an increase in femur-tibial bone mass by 3 months of age. This is contrary to several studies alluded to earlier. Increased bone volume (BV) was accompanied by a significant increase in cortical thickness of the femur and trabecular bone that extended beyond the epiphyseal area into the marrow cavity. Subsequent evaluations revealed an increase in osteoclast numbers with high Pb exposure, but a deficiency in osteoclastic activity. These findings were substantiated by observed increases in levels of the resorption-altering hormones calcitonin and estrogen. In addition we found that pro-osteoclastic nuclear factor-kappa beta (NF-κB) pathway activity was dose dependently elevated with Pb, both in vivo and in vitro. However, the ability of osteoclasts to resorb bone was depressed in the presence of Pb in media and within test bone wafers. These findings indicate that exposure to high Pb levels disrupts early life bone accrual that may involve a disruption of osteoclast activity. This study accentuates the dose dependent variation in Pb exposure and consequent effects on skeletal health.