Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibition of protein geranylgeranylation and farnesylation protects against graft-versus-host disease via effects on CD4 effector T cells

Despite advances in immunosuppressive regimens, acute graft-versus-host disease remains a frequent complication of allogeneic hematopoietic cell transplantation. Pathogenic donor T cells are dependent on correct attachment of small GTPases to the cell membrane, mediated by farnesyl- or geranylgeranyl residues, which, therefore, constitute potential targets for graft-versus-host disease prophylaxis. A mouse model was used to study the impact of a farnesyl-transferase inhibitor and a geranylgeranyl-transferase inhibitor on acute graft-versus-host disease, anti-cytomegalovirus T-cell responses and graft-versus-leukemia activity. Treatment of mice undergoing allogeneic hematopoietic cell transplantation with farnesyl-transferase inhibitor and geranylgeranyl-transferase inhibitor reduced the histological severity of graft-versus-host disease and prolonged survival significantly. Mechanistically, farnesyl-transferase inhibitor and geranylgeranyl-transferase inhibitor treatment resulted in reduced alloantigen-driven expansion of CD4 T cells. In vivo treatment led to increased thymic cellularity and polyclonality of the T-cell receptor repertoire by reducing thymic graft-versus-host disease. These effects were absent when squalene production was blocked. The farnesyl-transferase inhibitor and geranylgeranyl-transferase inhibitor did not compromise CD8 function against leukemia cells or reconstitution of T cells that were subsequently responsible for anti-murine cytomegalovirus responses. In summary, we observed an immunomodulatory effect of inhibitors of farnesyl-transferase and geranylgeranyl-transferase on graft-versus-host disease, with enhanced functional immune reconstitution. In the light of the modest toxicity of farnesyl-transferase inhibitors such as tipifarnib in patients and the potent reduction of graft-versus-host disease in mice, farnesyl-transferase and geranylgeranyl-transferase inhibitors could help to reduce graft-versus-host disease significantly without having a negative impact on immune reconstitution.
Reliability based design optimization for fine pitch ball grid array: Modeling construction and DOE analysis

This paper presents a reliability based optimization modeling approach demonstrated for the design of fine pitch ball grid array (fpBGA) structure. In this study the focus is on the strategy for handling the uncertainties in the package design inputs and their implementation into the design optimization modeling framework. The analysis of thermo-mechanical behavior of the package is carried out to predict the warpage behavior of the fpBGA package and the die surface stress. A 3D non-linear finite element model including appropriate information of packaging material properties is constructed to predict the thermal-mechanical behavior of fpBGA after molding process. Real samples are fabricated and undergone shadow moiré inspection and analysis to validate the finite element model. A screening experiment based on orthogonal DOE scheme procedure is carried out in order to find out the correlation between the response and input factors (including interactions) and defining their significance. Finally the most essential/significant input factors can be selected to conduct further analysis (response surface reconstruction, interpolation and optimization) with adding more simulation runs.
SUMO-1-Dependent Allosteric Regulation of Thymine DNA Glycosylase Alters Subnuclear Localization and CBP/p300 Recruitment

ABSTRACT Previous studies have demonstrated that the base excision repair enzyme thymine DNA glycosylase (TDG) mediates recruitment of histone acetyltransferases CREB-binding protein (CBP) and p300 to DNA, suggesting a plausible role for these factors in TDG-mediated repair. Furthermore, TDG was found to potentiate CBP/p300-dependent transcription and serve as a substrate for CBP/p300 acetylation. Here, we show that the small ubiquitin-like modifier 1 (SUMO-1) protein binding activity of TDG is essential for activation of CBP and localization to promyelocytic leukemia protein oncogenic domains (PODs). SUMO-1 binding is mediated by two distinct amino- and carboxy-terminal motifs (residues 144 to 148 and 319 to 322) that are negatively regulated by DNA binding via an amino-terminal hydrophilic region (residues 1 to 121). TDG is also posttranslationally modified by covalent conjugation of SUMO-1 (sumoylation) to lysine 341. Interestingly, we found that sumoylation of TDG blocks interaction with CBP and prevents TDG acetylation in vitro. Furthermore, sumoylation effectively abrogates intermolecular SUMO-1 binding and a sumoylation-deficient mutant accumulates in PODs, suggesting that sumoylation negatively regulates translocation to these nuclear structures. These findings suggest that TDG sumoylation promotes intramolecular interactions with amino- and carboxy-terminal SUMO-1 binding motifs that dramatically alter the biochemical properties and subcellular localization of TDG.
Communicative Behavior in the Natural Environment

Many individuals with developmental disabilities show delays in the development of sophisticated communication skills. Listeners often misunderstand their unconventional means of communication, and breakdowns occur. A communication repair is a second attempt to communicate a message when a breakdown is encountered. Qualitative methods were employed to assess and describe the communication repairs used by two young children with autism spectrum disorders who had limited expressive language. During natural observations, only one of the two participating children was observed to repair his initial requests. The results suggest that in contrast to the emphasis in the literature that development is the primary determinant of repair behavior, variables in the children's social and physical environment influenced their communication repair behavior. Implications for practice and research are described.
Design Principles for Real-Time Process Control Systems Report 94-42

This report discusses a framework for formal requirements and design speciication, and designing of Real-Time process Control systems (RTC-systems). The framework combines axiomatic with operational speciication approaches for expressing respectively requirements and design. In addition, a design method is discussed and the principles and necessary instrumentation it assumes are identiied and embedded in a notation based on ASTRAL 18]. This design method enables the incremental designing of total RTC-systems by interleaved application of top-down and bottom-up designing steps. Since the goal of the report is the identiication of the principles, and since it does not concern any speciic subclass of RTC-systems' requirements speciications, then the principles are kept as general as possible. When necessary, these general principles can be limited to any chosen subclass of these requirements speciications.
Evolution in Seismic Properties During Low and Intermediate Water Saturation: Competing Mechanisms During Water Imbibition?

Despite the efforts reported in the literature to explain contrasting experimental observations, the evolution of seismic attributes (velocity and attenuation) of rocks across the saturation range remains ill understood. In a comparative study, we monitored the evolution of ultrasonic P wave attributes in a porous sandstone subjected to two experiments of moisture adsorption and water spontaneous imbibition. Both experiments highlighted a significant (i.e., by 1 order of magnitude) and similar drop in P waves amplitudes, although the maximum saturations reached in each experiment is very different (i.e., about 2% vs. 70%). However, only moisture adsorption leads to a dramatic elastic softening (velocity reduction). This difference might be explained by the coupling between two competing physical mechanisms taking place during water imbibition, namely, elastic softening driven by water adsorption at the grain contact and elastic stiffening driven by full saturation of the grain contacts, at the ultrasonic frequency of the measurement.
MOBILE ROBOT NAVIGATION USING MONTE CARLO LOCALIZATION

This paper presents an algorithm for the mobile navigation of a robot using Monte Carlo. Previously, people did a lot of work for the tracking of mobile robot. Previously people used grid-based approach that used high resolution 3D grids to represent the state space. Whereas, this method is computationally quite efficient. Using Monte Carlo Localization we apply the sampling approach to divide the state space into samples. We can increase the number of samples where required. Monte Carlo Localization is easy to implement. Several results proved that Monte Carlo yields more accurate results. And also, it is computationally very efficient.
Applying hypertext and hypermedia to scholarly journals enables both product and process innovation

Abstract: Early uses of hypertext technologies were associated with scholarly communication. Newelectronic-only journals have been quick to adopt hypertext/hypermedia technologies. Existing printjournals have also started to adopt such technologies as they make the transition to parallel delivery. Thewidespread uptake of the World Wide Web has enabled journals to improve, enhance and transform whatthey do. This paper surveys these developments and places them in context.Categories and Subject Descriptors: J.7 [Computers in Other Systems] : Publishing General Terms: Hypertext, HypermediaAdditional Key Words and Phrases: Scholarly communication, Electronic journals, Parallel publishing Some history Hypertext can be broadly defined as the combination of hypertext linking mechanisms and multimedia content[Bolter 1991, pp. 25-7]. We think of hypermedia as exemplifying the sort of rich information that the computer hasmade possible. And yet, hypertext and hypermedia predate the computer, in some cases to a significant extent.Hebrew citation indexes in mediaeval editions of the Talmud are an example of rich pre-computer hypertext[Weinberg 1997] and Paul Otlet's work at the International Offices of Documentation [Rayward 1997] can be seenas an early manual hypermedia system. Even the citations to print sources used in this article can be regarded as aslow and clumsy form of hypertext. __________________________
Exploring students' scientific understandings in different educational contexts using a cognitive structural model

Students enter school with well-developed ideas about scientific concepts formulated predominantly through their sensory experiences with their natural world. While these conceptions are coherent and make sense to the student, they may differ significantly from the scientific view. The role of the teacher is to interpret what the student understands and to implement teaching experiences to support students in building or constructing their scientific knowledge. In this paper we explore the conceptual understandings of day and night held by Years 5–6 students in China (N=150) and Australia (N=205). By using the Structure of the Observed Learning Outcome (SOLO) model, students’ extended responses were coded into modes and levels of thinking based on the structure of understanding demonstrated. Analysis of the results produced a conceptual pathway for day and night that was shared by students across the two countries. With this pathway identified it is possible to consider how teaching might support students in providing not only a variety of learning opportunities but also degrees of complexity within these to cater for a range of students. Conceptual Understanding in Science The major premise of constructivism underpinning learning in science is that individuals construct meanings or ‘versions of reality’ based upon their own experiences and pre-existing knowledge. In this context, learning is no longer about knowledge acquisition by a passive learner but an active process requiring the learner to construct new ideas or restructure existing ones (Costa, Hughes, & Pinch, 1998). However, developing understandings in science is fraught with difficulties. For instance, what happens when students construct concepts that are not scientifically accurate? Within the science education literature these instances are referred to as ‘alternative conceptions’ and there is a large body of research around the area conducted in the 1980s and 1990s (Pfundt & Duit, 1994). A review of a
The ATP‐binding cassette transporter–encoding gene CgSNQ2 is contributing to the CgPDR1‐dependent azole resistance of Candida glabrata

Our previous investigation on Candida glabrata azole‐resistant isolates identified two isolates with unaltered expression of CgCDR1/CgCDR2, but with upregulation of another ATP‐binding cassette transporter, CgSNQ2, which is a gene highly similar to ScSNQ2 from Saccharomyces cerevisiae. One of the two isolates (BPY55) was used here to elucidate this phenomenon. Disruption of CgSNQ2 in BPY55 decreased azole resistance, whereas reintroduction of the gene in a CgSNQ2 deletion mutant fully reversed this effect. Expression of CgSNQ2 in a S. cerevisiae strain lacking PDR5 mediated not only resistance to azoles but also to 4‐nitroquinoline N‐oxide, which is a ScSNQ2‐specific substrate. A putative gain‐of‐function mutation, P822L, was identified in CgPDR1 from BPY55. Disruption of CgPDR1 in BPY55 conferred enhanced azole susceptibility and eliminated CgSNQ2 expression, whereas introduction of the mutated allele in a susceptible strain where CgPDR1 had been disrupted conferred azole resistance and CgSNQ2 upregulation, indicating that CgSNQ2 was controlled by CgPDR1. Finally, CgSNQ2 was shown to be involved in the in vivo response to fluconazole. Together, our data first demonstrate that CgSNQ2 contributes to the development of CgPDR1‐dependent azole resistance in C. glabrata. The overlapping in function and regulation between CgSNQ2 and ScSNQ2 further highlight the relationship between S. cerevisiae and C. glabrata.
Bit error rates for a MIMO system in Rayleigh and Rician channels

Multiple input multiple output (MIMO) systems, being under intensive research nowadays, are discussed under the aspect of bit error rates (BER) that can be achieved in indoor scenarios. This paper focuses on the influence of antenna diversity, a line of sight (LOS) signal and channel estimation errors onto the BER using BPSK and 16-QAM modulation. The developed simulation environment allows us to determine the necessary accuracy for channel estimation to achieve the desired BER performance in a MIMO channel under certain constraints like number of antennas, modulation scheme etc.
Orthostatic Intolerance Is Independent of the Degree of Autonomic Cardiovascular Adaptation after 60 Days of Head-Down Bed Rest

Spaceflight and head-down bed rest (HDBR) can induce the orthostatic intolerance (OI); the mechanisms remain to be clarified. The aim of this study was to determine whether or not OI after HDBR relates to the degree of autonomic cardiovascular adaptation. Fourteen volunteers were enrolled for 60 days of HDBR. A head-up tilt test (HUTT) was performed before and after HDBR. Our data revealed that, in all nonfainters, there was a progressive increase in heart rate over the course of HDBR, which remained higher until 12 days of recovery. The mean arterial pressure gradually increased until day 56 of HDBR and returned to baseline after 12 days of recovery. Respiratory sinus arrhythmia and baroreflex sensitivity decreased during HDBR and remained suppressed until 12 days of recovery. Low-frequency power of systolic arterial pressure increased during HDBR and remained elevated during recovery. Three subjects fainted during the HUTT after HDBR, in which systemic vascular resistance did not increase and remained lower until syncope. None of the circulatory patterns significantly differed between the fainters and the nonfainters at any time point. In conclusion, our data indicate that the impaired orthostatic tolerance after HDBR could not be distinguished by estimation of normal hemodynamic and/or neurocardiac data.
Design and development of an underactuated prosthetic hand

Current prosthetic hands are basically simple grippers with one or two degrees of freedom, which barely restore the capability of the thumb-index pinch. Although most amputees consider this performance as acceptable for usual tasks, there is ample room for improvement by exploiting recent progresses in mechatronic design and technology. This paper focus on an innovative approach for the design and development of prosthetic hands based on underactuated mechanisms. Furthermore, it describes the development and a preliminary analysis of a first prototype of an underactuated prosthetic hand.
A key-revocable attribute-based encryption for mobile cloud environments

In this paper, we propose a new Attribute-Based Encryption (ABE) scheme applicable to mobile cloud environments. A key issue in mobile cloud environments is how to reduce the computational cost on mobile devices and delegate the remaining computation to cloud environments. We also consider two additional issues: an efficient key revocation mechanism for ABE based on a concept of token-controlled public key encryption, and attribute hiding encryption from a cloud server. To reduce the computational cost on the client side, we propose an efficient ABE scheme jointly with secure computing on the server side. We analyze the security of our ABE scheme and evaluate the transaction time of primitive functions implemented on an Android mobile device and a PC. The transaction time of our encryption algorithm is within 150 msec for 89-bit security and about 600 msec for 128-bit security on the mobile device. Similarly, the transaction time of the decryption algorithm is within 50 msec for 89-bit security and 200 msec for 128-bit security.
The Dimensionality of Self-Rated Employee Performance: An Integrated View

This study addresses the debate in the literature regarding the dimensionality of the job performance construct. The sample comprised 647 public servants from a state-based law enforcement organisation. Exploratory and confirmatory factor analyses delineate a four:factor structure, consisting of in-role behaviour, organisational citizenship behaviour directed towards (1) individuals or (2) the organisation, and a distinct latent variable deemed counter-productive work behaviour (CWB). The pattern of correlations among the four performance dimensions and between the performance dimensions and attitudes support the construct and discriminant validity of the four performance dimensions. Further, the . findings propose that CWB is a core, not discretionary, dimension of performance.
Interactive search in large video collections

We present a search interface for large video collections with time-aligned text transcripts. The system is designed for users such as intelligence analysts that need to quickly find video clips relevant to a topic expressed in text and images. A key component of the system is a powerful and flexible user interface that incorporates dynamic visualizations of the underlying multimedia objects. The interface displays search results in ranked sets of story keyframe collages, and lets users explore the shots in a story. By adapting the keyframe collages based on query relevance and indicating which portions of the video have already been explored, we enable users to quickly find relevant sections. We tested our system as part of the NIST TRECVID interactive search evaluation, and found that our user interface enabled users to find more relevant results within the allotted time than those of many systems employing more sophisticated analysis techniques.
Phenelzine, mood response, and sleep.

The monoamine oxidase inhibitor antidepressant, phenelzine, will abolish the signs of REM sleep after a delay of days or weeks. In a study utilizing 22 patients suffering from endogenous depression, the time course of mood change was related to the time course of the induced REM sleep change. A period of placebo administration preceded administration of 60, 75, or 90 mg of phenelzine dihydrogen sulfate daily. Patient self-ratings of mood were employed and independent judges selected the date of onset of sustained mood improvement with at least three out of four in agreement for nine of the patients. In these nine depressed patients, restoration of normal mood coincided with the delayed abolition of the signs of REM sleep.
You Have My Word: Reciprocity Expectation Modulates Feedback-Related Negativity in the Trust Game

Promise is one of the most powerful tools producing trust and facilitating cooperation, and sticking to the promise is deemed as a key social norm in social interactions. The present study explored the extent to which promise would influence investors’ decision-making in the trust game where promise had no predictive value regarding trustees’ reciprocation. In addition, we examined the neural underpinnings of the investors’ outcome processing related to the trustees’ promise keeping and promise breaking. Consistent with our hypothesis, behavioral results indicated that promise could effectively increase the investment frequency of investors. Electrophysiological results showed that, promise induced larger differentiated-FRN responses to the reward and non-reward discrepancy. Taken together, these results suggested that promise would promote cooperative behavior, while breach of promise would be regarded as a violation of the social norm, corroborating the vital role of non-enforceable commitment in social decision making.
Feedback Control of Computing Systems

Preface. PART I: BACKGROUND. 1. Introduction and Overview. PART II: SYSTEM MODELING. 2. Model Construction. 3. Z-Transforms and Transfer Functions. 4. System Modeling with Block Diagrams. 5. First-Order Systems. 6. Higher-Order Systems. 7. State-Space Models. PART III: CONTROL ANALYSIS AND DESIGN. 8. Proportional Control. 9. PID Controllers. 10. State-Space Feedback Control. 11. Advanced Topics. Appendix A: Mathematical Notation. Appendix B: Acronyms. Appendix C: Key Results. Appendix D: Essentials of Linear Algebra. Appendix E: MATLAB Basics. References. Index.
Iterated Ritz Method for solving systems of linear algebraic equations

U radu je opisano stanje razvoja nove iteracijske metode za rjesavanje sustava linearnih algebarskih jednadžbi. Metoda je pogodna za izrazito velike sustave slabo popunjenih matrica. Osim vlastitih obilježja, posjeduje i svojstvo opcenitosti, jer su mnogi iteracijski postupci samo poseban slucaj ovoga pristupa. Algoritam je realiziran samostalno, a potom je pridružen programu otvorena koda FEAP. Provedene su i raznolike provjere, posebice na prakticnim modelima. Premda je postupak tek djelomice istražen, vec pokazuje dobre rezultate.
Solid Sampling with a Diode Laser for Portable Ambient Mass Spectrometry

A hand-held diode laser is implemented for solid sampling in portable ambient mass spectrometry (MS). Specifically, a pseudocontinuous wave battery-powered surgical laser diode is employed for portable laser diode thermal desorption (LDTD) at 940 nm and compared with nanosecond pulsed laser ablation at 2940 nm. Postionization is achieved in both cases using atmospheric pressure photoionization (APPI). The laser ablation atmospheric pressure photoionization (LAAPPI) and LDTD-APPI mass spectra of sage leaves (Salvia officinalis) using a field-deployable quadrupole ion trap MS display many similar ion peaks, as do the mass spectra of membrane grown biofilms of Pseudomonas aeruginosa. These results indicate that LDTD-APPI method should be useful for in-field sampling of plant and microbial communities, for example, by portable ambient MS. The feasibility of many portable MS applications is facilitated by the availability of relatively low cost, portable, battery-powered diode lasers. LDTD could also be coupled with plasma- or electrospray-based ionization for the analysis of a variety of solid samples.