Miracle-dz/UniFAR-FTUs huggingface dataset:
A randomized double-blind, placebo-controlled trial of venlafaxine-extended release for co-occurring cannabis dependence and depressive disorders.

AIM To evaluate whether venlafaxine-extended release (VEN-XR) is an effective treatment for cannabis dependence with concurrent depressive disorders. DESIGN This was a randomized, 12-week, double-blind, placebo-controlled trial of out-patients (n = 103) with DSM-IV cannabis dependence and major depressive disorder or dysthymia. Participants received up to 375 mg VEN-XR on a fixed-flexible schedule or placebo. All patients received weekly individual cognitive-behavioral psychotherapy that primarily targeted marijuana use. SETTINGS The trial was conducted at two university research centers in the United States. PARTICIPANTS One hundred and three cannabis-dependent adults participated in the trial. MEASUREMENTS The primary outcome measures were (i) abstinence from marijuana defined as at least two consecutive urine-confirmed abstinent weeks and (ii) improvement in depressive symptoms based on the Hamilton Depression Rating Scale. FINDINGS The proportion of patients achieving a clinically significant mood improvement (50% decrease in Hamilton Depression score from baseline) was high and did not differ between groups receiving VEN-XR (63%) and placebo (69%) (χ1 (2) = 0.48, P = 0.49). The proportion of patients achieving abstinence was low overall, but was significantly worse on VEN-XR (11.8%) compared to placebo (36.5%) (χ1 (2) = 7.46, P < 0.01; odds ratio = 4.51, 95% confidence interval: 1.53, 13.3). Mood improvement was associated with reduction in marijuana use in the placebo group (F1,179 = 30.49, P < 0.01), but not the VEN-XR group (F1,186 = 0.02, P = 0.89). CONCLUSIONS For depressed, cannabis-dependent patients, venlafaxine-extended release does not appear to be effective at reducing depression and may lead to an increase in cannabis use.
The Impact of Farm Animal Housing Restrictions on Egg Prices, Consumer Welfare, and Production in California

&NA; New animal welfare policies on the horizon in many U.S. states have prompted debates about the cost of achieving happier hens and hogs. A recent policy change in California offers a unique opportunity to measure the economic repercussions of minimum space requirements for egg‐laying hens. Using forecasting methods and structural break tests as applied to sixteen years of monthly data on egg production and input prices, we find that by July 2016, both egg production and the number of egg‐laying hens were about 35% lower than they would have been in the absence of the new regulations. Out‐of‐state eggs were able to compensate for falling California production until around the time the new rules were implemented, at which point imports of eggs into California fell. For consumers, we estimate price impacts using panel structural break tests and difference‐in‐differences models as applied to five years of scanner data from the retail market for shell eggs in three California markets and three control markets. We find that the average price paid per dozen eggs was about 22% higher from December 2014 through September 2016 than it would have been in the absence of the hen housing restrictions. The price impact fell over time, from an initial impact of about 33% per dozen to about 9% over the last six months of the observed time horizon. These price increases correspond to welfare losses of at least $117 million for the three California markets over the observed time horizon. Our results suggest that because of the policy change, California consumers can expect to experience annual welfare losses of at least $25 million in future years from higher retail egg prices alone.
Hoxd13 and Hoxa13 Directly Control the Expression of the EphA7 Ephrin Tyrosine Kinase Receptor in Developing Limbs*

Hoxa and Hoxd genes, related to the Drosophila Abd-B gene, display regionally restricted expression patterns and are necessary for the formation of the limb skeletal elements. Hox genes encode transcription factors, which are supposed to control the expression of a series of downstream target genes, whose nature has remained largely elusive. Several genes were identified that are differentially expressed in relation to Hox gene activity; few studies, however, explored their direct regulation by Hox proteins. Ephrin tyrosine kinase receptors and ephrins have been proposed as Hox targets, and recently, evidence was gained for their role in limb development. The expression of the EphA7 gene in developing limbs was shown to correlate with the expression of Hoxa13 and Hoxd13; however, its direct regulation by these genes has never been assessed. We have characterized the EphA7 promoter region and show that it contains multiple binding sites for paralog group 13 Hox proteins. We found that one of these sites is bound in vivo by HOXA13 and HOXD13 and by endogenous Hoxd13 in developing mouse limbs. Moreover, we show that HOXD13 and HOXA13 activate transcription from the EphA7 promoter and that a mutation of the HOXA13/HOXD13 binding site was sufficient to abolish activation. Conversely, the HOXD13(147L) mutation, identified in patients displaying a novel brachydactyly-polydactyly syndrome, does not bind to in vivo, and fails to transactivate the EphA7 promoter. These results establish that EphA7 is a direct downstream target of Hoxd13 and Hoxa13 during limb development, thus providing further insight into the regulatory networks that control limb patterning.
Molecular requirement for interleukin 1 induction by lipopolysaccharide‐stimulated human monocytes: Involvement of the heptosyl‐2‐keto 3‐deoxyoctulosonate region

Experiments were undertaken to localize in the lipopolysaccharide (LPS) the minimal structural determinants sufficient to initiate the signal leading to interleukin 1 (IL 1) secretion by human monocytes. Our results clearly demonstrated that this signal is triggered by structures present in the so‐called inner‐core region which chemically consists of 2‐keto‐3‐deoxy‐wD‐manno‐octulosonic acid (KDo) and heptose in many LPS of gram‐negative bacteria. Thus, the isolated polysaccharide region of Bordetella pertussis endotoxin as well as fragments derived therefrom containing the reducing KDO unit were able to induce similar levels of IL1 induction as the native LPS. Similarly, the trisaccharide α‐D‐manno‐heptopyranosyl‐(1–3)‐α‐D‐manno‐heptopyranosyl‐(1–5)‐3‐deoxy‐D‐manno‐octulosonic acid (hep‐hep‐KDO), representative for the inner‐core region of a large number of enterobacterial LPS, was a very potent IL 1 inducer. Neither KDO monosaccharide, nor the α‐(2–4)‐linked 3‐deoxy‐D‐manno‐octulosonic acid disaccharide isolated from Salmonella rough‐form LPS promoted the signal indicating that the minimal structure of endotoxin able to induce IL 1 secretion resides in the hep (1–5)‐KDO disaccharide.
Dynamic cerebral autoregulation to induced blood pressure changes in human experimental and clinical sepsis

Previous studies have demonstrated that dynamic cerebral autoregulation to spontaneous fluctuations in blood pressure is enhanced following lipopolysaccharide (LPS) infusion, a human experimental model of early sepsis, whereas by contrast it is impaired in patients with severe sepsis or septic shock. In this study, we hypothesized that this pattern of response would be identical during induced changes in blood pressure. Dynamic cerebral autoregulation was assessed in nine healthy volunteers and six septic patients. The healthy volunteers underwent a 4‐h intravenous infusion of LPS (total dose: 2 ng kg−1). Mean arterial blood pressure (MAP, arterial transducer) and middle cerebral artery blood flow velocity (MCAv, transcranial Doppler ultrasound) were recorded continuously during thigh‐cuff deflation‐induced changes in MAP for the determination of a modified rate of regulation (RoR). This was performed before and after LPS infusion in healthy volunteers, and within 72 h following clinical diagnosis of sepsis in patients. In healthy volunteers, thigh‐cuff deflation caused a MAP reduction of 16 (13–20) % at baseline and 18 (16–20) % after LPS, while the MAP reduction was 12 (11–13) % in patients (P<0·05 versus volunteers at baseline; P<0·01 versus volunteers after LPS). The corresponding RoR values increased from 0·46 (0·31–0·49) s−1 at baseline to 0·58 (0·36–0·74) s−1 after LPS (P<0·05) in healthy volunteers, whereas they were similar to values observed in patients [0·43 (0·36–0·52) s−1; P = 0·91 versus baseline; P = 0·14 versus LPS]. While our findings support the concept that dynamic cerebral autoregulation is enhanced during the very early stages of sepsis, they remain inconclusive with regard to more advanced stages of disease, because thigh‐cuff deflation failed to induce sufficient MAP reductions in patients.
Antibody to hepatitis C virus second envelope (HCV‐E2) glycoprotein: A new marker of HCV infection closely associated with viremia

The second envelope protein (E2) of the hepatitis C virus (HCV) was cloned and expressed in Chinese hamster ovary (CHO) cells. This E2 glycoprotein was purified using ion exchange and lectin chromatography and used to construct an enzyme immunoassay for HCV E2 antibodies. The assay was shown to have good specificity, and detection of E2 antibodies was positively correlated (97.3%) to the presence of HCV RNA in serum and plasma. A high concordance between HCV 2.0 and E2 EIA reactivities was also observed. E2 antibody was the first serological marker to appear in 3/5 HCV seroconversion panels. This work demonstrated that 42.4% of core and 15.4% of NS3 indeterminate specimens also contained antibodies to E2, suggesting that HCV infection had occurred in these individuals. The E2 antibody assay was used to evaluate HCV 2.0 EIA‐positive, HCV 3.0 EIA‐negative plasma donors with indeterminate reactivity on RIBA HCV 2.0 or MATRIX HCV 1.0. Several HCV 3.0‐negative specimens were shown to contain E2 antibodies in addition to an original indeterminate serological marker, primarily core. It is concluded that anti‐E2 is a useful marker for determining HCV infection, and that the presence of antibodies to two nonoverlapping viral gene products suggests true HCV exposure. New HCV 3.0 blood screening tests should detect HCV 2.0‐positive donors who present with an indeterminate pattern by RIBA or MATRIX and who also carry E2 antibodies. © 1995 Wiley‐Liss, Inc.
Fertility preservation in the female cancer patient

Hundreds of thousands of young women are diagnosed with cancer each year, but due to advances in screening, diagnosis, and treatment, survival rates have improved dramatically. With improved survival, long‐term effects of cancer treatment including infertility need to be addressed and should be discussed as soon as possible. Oncologists should be familiar with their patients' risks of infertility and available options for fertility preservation and future reproduction. J. Surg. Oncol. 2014; 110:907–911. © 2014 Wiley Periodicals, Inc.
The Virtue of Reverence in Interdisciplinary Studies.

One reason scholars and students are attracted to interdisciplinary studies and its research process is because doing good work in the field requires values, traits and skills that are virtuous rather than vicious. This study examines how successful interdisciplinary research is intrinsically related to the human capacity for reverence in the face of complexity. Articulating reverence in the research process advances our understanding of interdisciplinary theories concerning complexity, perspective taking, common ground and integration. The result helps balance the cognitive emphasis in interdisciplinary studies with an account emphasizing emotion and character. Interdisciplinary reverence illuminates the kind of integration practiced in the humanities and fine and performing arts.
A Conceptual Framework of Festival Visitors’ Behavioral Intentions.

The purpose of this article is to develop a conceptual framework to determine the factors influencing visitors’ behavioral intention to attend festivals and events. The theoretical framework focuses on the relationship between experience quality, perceived value, satisfaction and behavioral intentions of the festival tourists. The diversity of growth and popularity of festivals and events have been growing and many communities are arranging different festivals and events as leisure and cultural recreation for their community members. Such arrangements have led to improvements in both local community lives and image. In order to increase visitor participation, it is important that they perceive good value in relation to their satisfaction and high quality in services. From previous research, it was evident that service quality, perceived value and satisfaction are some of the major factors that influence tourists’ behavioral intentions. In the context of festival tourism, little research has been conducted to test the relationship between quality, value, satisfaction and behavioral intention. In this paper a conceptual model has been proposed to examine whether experience quality has an impact on perceived value, which in turn is posited herein to affect satisfaction and behavioral intention. Several hypotheses have been proposed to test a research model in the context of festival tourism. In an ensuing study, the proposed model will further be tested empirically at a music festival named Malakoff occurring yearly in western Norway. The festival generates considerable interest and regional and national participation within the region.
A Content Analysis of the Domains of Advocacy Competencies in Select Counseling Journals: 2004–2016

ABSTRACT The 2003 ACA Advocacy Competencies include domains in which counselors can act with or on behalf of clients, students, schools, communities, and the public. We completed a content analysis of 23 counseling journals (2004–2016) to explore how the counseling literature infused dimensions of ACA Advocacy Competencies. Out of 6,945 journal articles over the 13-year period, 280 were relevant for our study. Findings and suggestions related to the Advocacy Competencies framework are provided.
Extracellular p53 fragment re-enters K-Ras mutated cells through the caveolin-1 dependent early endosomal system

K-Ras mutation is detected in over 30% of human malignancies. In particular, 90% of human pancreatic cancers are initiated by K-Ras mutation. Thus, selective elimination of K-Ras mutated cells would be a plausible strategy to prevent or cure the malignancies. In our previous reports, it has been revealed that oncogenic K-Ras promotes the exocytosis of p53 with Snail. In this study, we have followed the final destination of extracellular p53, which is secreted by the Snail complex. Here we provide evidences that p53, exported from K-Ras-mutated cells, is specifically re-endocytosed by oncogenic K-Ras-containing cancer cells. The p53 DNA-binding domain directly associates with caveolin-1 and enters K-Ras mutated cells through early endosome-mediated endocytosis. Using a serial deletion approach, we revealed that a fragment of human p53 extending from 93-143 amino acids (AA) is responsible for binding with caveolin-1 and for endocytosis. In contrast, p53-Snail binding occurs at the 143-193 aa region. Finally, through in vivo study, we confirmed that injected recombinant p53 could be up-taken by tumor tissues, constructed by oncogenic K-Ras transformed MEF cells. In contrast, the tumors formed by H-Ras mutated MEF cells did not accumulate the injected p53 protein. These results indicate that the p53 fragment might be useful as a specific delivery tool into K- Ras mutated cells as well as a diagnostic method.
Optimal Test Points Selection Based on Multi-Objective Genetic Algorithm

A new approach to select an optimal set of test points is proposed. The described method uses fault-wise table and multi- objective genetic algorithm to find the optimal set of test points. First, the fault-wise table is constructed whose entries are measurements associated with faults and test points. The problem of optimal test points selection is transformed to the selection of the columns that isolate the rows of the table. Then, four objectives are described according to practical test requirements. The multi-objective genetic algorithm is explained. Finally, the presented approach is illustrated by a practical example. The results indicate that the proposed method efficiently and accurately finds the optimal set of test points and is practical for large scale systems.
Age-Dependent Acoustic and Microelastic Properties of Red Blood Cells Determined by Vector Contrast Acoustic Microscopy

Abstract Variations of the mechanical properties of red blood cells that occur during their life span have long been an intriguing task for investigations. The research presented is based on noninvasive monitoring of red blood cells of different ages performed by scanning acoustic microscopy with magnitude and phase contrast. The characteristic signature of fixed cells from groups of three different ages fractionated according to mass density is obtained from the acoustic microscope images, with the data represented in polar graphs. The analysis of these data enables the determination of averaged values for the velocities of ultrasound propagating in the cells from the different groups ranging from (1,681 ± 16) m s−1 in the youngest to (1,986 ± 20) m s−1 in the oldest group. The determined bulk modulus varies with age from (3.04 ± 0.05) GPa to (4.34 ± 0.08) GPa. An approach to determine for an age-mixed population of red blood cells, collected from a healthy person, the age of the individual cells and the age dependence of the cell parameters including density, velocity, and attenuation of longitudinal polarized ultrasonic waves traveling in the cells is demonstrated.
KANADI and Class III HD-Zip Gene Families Regulate Embryo Patterning and Modulate Auxin Flow during Embryogenesis in Arabidopsis[W][OA]

Embryo patterning in Arabidopsis thaliana is highly affected when KANADI or Class III HD-Zip genes are compromised. Triple loss-of-function kan1 kan2 kan4 embryos exhibit striking defects in the peripheral–central axis, developing lateral leaf-like organs from the hypocotyls, whereas loss of Class III HD-Zip gene activity results in a loss of bilateral symmetry. Loss of KANADI activity in a Class III HD-Zip mutant background mitigates the defects in bilateral symmetry, implying that the two gene families act antagonistically during embryonic pattern formation. Dynamic patterns of auxin concentration and flux contribute to embryo patterning. Polar cellular distribution of PIN-FORMED1 (PIN1) mediates auxin flow throughout embryogenesis and is required for establishment of the apical–basal axis and bilateral symmetry. Defects in the pattern of PIN1 expression are evident when members of either the KANADI or Class III HD-Zip gene families are compromised. Abnormal expression patterns of PIN1 in KANADI or Class III HD-Zip multiple mutants and the phenotype of plants in which members of both gene families are mutated suggest that pattern formation along the central–peripheral axis results from interplay between auxin and the KANADI and Class III HD-Zip transcription factors, whose defined spatial and temporal expression patterns may also be influenced by auxin.
Web-based application services composition for e-business

Web service is becoming an important approach for enterprises to implement their business applications on Internet. However, most of Web services are built based on special business function, they are isolated and do not address the information communication for integrating the business processes. In this paper, we propose a new composition framework, which comprises the Web-based semantic definition for business messages, the methodology of constructing visualized integrated processes based on Business Process Execution Language (BPEL), and the strategy of vocabulary-based business rules for e-business. In addition, we present a case study that demonstrates the way of composing different kind of application services to a uniform service
Heterodisaccharide 4‐O‐(N‐acetyl‐β‐d‐glucosaminyl)‐d‐glucosamine is an effective chemotactic attractant for Vibrio bacteria that produce chitin oligosaccharide deacetylase

Aims: To investigate the attractant effect of 4‐O‐(N‐acetyl‐β‐d‐glucosaminyl)‐d‐glucosamine (GlcNAc‐GlcN) in the chemotaxis of Vibrio bacteria that produce carbohydrate esterase (CE) family 4 chitin oligosaccharide deacetylase (COD), an enzyme that catalyzes the production of GlcNAc‐GlcN from N,N′‐diacetylchitobiose (GlcNAc)2.
The EMU after the Euro crisis : lessons and possibilities : findings and proposals from the Horizon 2020 ADEMU project

33 Great Sutton Street London EC1V 0DX Tel: +44 (0)20 7183 8801 Email: cepr@cepr.org www.cepr.org This eBook provides an overview of the findings and proposals of the Horizon 2020 ADEMU research project (June 2015 – May 2018). The main aim of the project was to reassess the fiscal and monetary framework of the European Economic Monetary Union (EMU) after the euro crisis, when this framework is still in transition and under debate. The research has used, and has further developed, the tools and theories of modern economics and finance, as well as of modern legal research, with three aims:
Adaptive Distributed Precoding Scheme Based on Gradient Iteration for CoMP Systems

An adaptive distributed precoding scheme is proposed for coordinated multi-point transmission systems. The precoding vector used by coordinated base station (BS) is determined by adaptive gradient iteration according to the perturbation vector and adjustment factor. The user equipment only feeds one quantized adjustment factor back to each coordinated BS. The adjustment factor is selected based on the precoding vector of the coordinated BS in the previous frame and the perturbation vector predefined in this frame. The proposed scheme takes advantage of the spatial non-correlation and temporal correlation of the distributed MIMO channel. The design of the perturbation vector set is given. Simulation results show that the proposed scheme has a good trade-off between system performance and the system control feedback overhead.
Territorial servitization in Marshallian industrial districts: the industrial district as a place-based form of servitization

ABSTRACT This paper is the first general study of territorial servitization in Marshallian industrial districts (MIDs). It introduces the novel idea of place-based servitization as a form of territorial servitization, which is then used conceptually to reframe the traditional perspective on MIDs, manufacturing places par excellence. Drawing on comparable quantitative data from Italy and Spain for 1991, 2001 and 2011, the paper then provides generalizable empirical evidence about the stylized facts of servitization in MIDs, casting a light on the transition to services of the MID as one model of place-based servitization.