Miracle-dz/UniFAR-FTUs huggingface dataset:
β-Adrenoceptor-Mediated Relaxation of Carbachol-Pre-Contracted Mouse Detrusor

Aims: To study the β-adrenoceptor subtypes involved in the relaxation responses to (-)-isoprenaline in carbachol-pre-contracted (CCh) mouse detrusor muscle with intact and denuded mucosa. Methods: Isolated muscle strips from the urinary bladder of male C57BL6 mice or β2-adrenoceptor knockout mice were pre-contracted with CCh, 1 µM and relaxed with increasing concentrations of the β-adrenoceptor (β-AR) agonist (-)-isoprenaline and forskolin. For estimating the β-AR subtypes involved, subtype-selective receptor blockers were used, that is, CGP 20712A (β1-ARs), ICI 118,551 (β2-ARs), and L748,337 (β3-ARs). Results: Unlike in KCl-pre-contracted muscle, the mucosa did not affect the sensitivity of the relaxation response to (-)-isoprenaline in CCh-pre-contracted murine detrusor strips. Increasing concentrations of (-)-isoprenaline produced a biphasic concentration-relaxation response without any difference both during the presence and absence of mucosa. The relaxation fraction produced by low (-)-isoprenaline concentrations was mediated by β2-AR as evidenced by a shift of the concentration-response curve to higher concentrations with ICI 118,551, but not with CGP 20712A and L748,337, and by the absence of this fraction in β2-AR-KO mice. The relaxation response with low sensitivity to (-)-isoprenaline was not affected by any of the β-AR subtype-selective blockers and was the only response detected in detrusor strips from β2-AR-KO mice. Conclusions: In CCh-pre-contracted mouse detrusor, β2-ARs are responsible for the relaxation component with high sensitivity to (-)-isoprenaline as indicated by the conversion of a biphasic into a monophasic CRC with ICI 118,551 or by its absence in β2-AR KO mice. The mucosa does not impair relaxation under these conditions.
Single- and double-photoionization cross sections of atomic nitrogen from threshold to 31.

The relative photoionization cross section of atomic nitrogen for the production of singly and doubly charged ions has been measured from 44.3 to 275 A and from 520 to 852 A. The results have been made absolute by normalization to one-half of the molecular nitrogen cross section at short wavelengths. The smoothed atomic nitrogen cross sections sigma can be accurately represented, at short wavelengths, by the equation sigma(Mb) = 36,700 x (E exp-2.3) as a function of the photon energy E (eV), thereby allowing the cross sections to be extrapolated to the nitrogen K edge at 31 A.
Detection and removal of cloud contamination from AVHRR images

Describes the characteristics and performance of an algorithm designed to remove the effects of cloud contamination from AVHRR composite images over land. Using information in the temporal NDVI profile, the algorithm detects cloud-contaminated pixels, and optionally replaces these with interpolated values of individual AVHRR channels or channel transformations. As tested, the algorithm detects only those effects of residual clouds that result in a temporary decrease of the NDVI. It was found that the algorithm is capable of identifying clouds of varying opacity as well as cloud shadows present in the composites, and that its use appears preferable to compositing over longer periods. The algorithm can be applied without the need for ancillary information, e.g., on expected surface radiance conditions. Although developed for use in biospheric studies in Canada, it could also be applied at other latitudes where the seasonal trajectories of satellite-derived variables exhibit a single maximum or, by extension, a single minimum. >
Multi-stage reactive power programming using genetic algorithm

Reactive power programming is a multistage multi-objectives mixed integer programming problem. In this paper, a two steps procedure is proposed to solve the reactive power programming problem. First, a genetic algorithm is adopted aimed at minimizing the cost of power loss and investment at each stage. Then a dynamic programming is adopted aimed at finding the optimal policy of the whole stages. Results are presented of applying the proposed methodology to several power systems.
Memory functions, alprazolam and exposure therapy: a controlled longitudinal study of agoraphobia with panic disorder

Synopsis Benzodiazepines (BZs) produce transient anterograde amnesia when given to normal subjects. The present longitudinal study assessed whether BZs impair memory functions in a clinically anxious group. Eighty-two agoraphobics with panic disorder were randomly allocated to one of four treatment groups resulting from a combination of two drug treatments (alprazolam or placebo) and two psychological treatments (exposure or relaxation). Of these, 38 subjects were assessed on a range of objective and subjective indices of memory and mood at three time points: before treatment, after 8 weeks of treatment and again at 24 weeks when patients had been free of medication from 5–8 weeks. Alprazolam produced pronounced impairments on a word recall task. At the 24-week medication-free follow-up, alprazolam patients were still impaired on the task compared with placebo patients. Alprazolam did not impair performance on an implicit memory task and did not affect digit span. Differences between psychological treatments emerged mainly in subjective memory effects. Findings are discussed in terms of the specificity of BZ-induced amnesia and differential tolerance to the varying effects of BZs. Implications are drawn out for the patient's ability to function optimally in daily life while taking alprazolam.
BST-2 Diminishes HIV-1 Infectivity

ABSTRACT Bone marrow stromal cell antigen 2 (BST-2, also known as tetherin/CD317/HM1.24) inhibits the release of human immunodeficiency virus type 1 (HIV-1) and other enveloped viruses by tethering virus particles to the cell surface. In this study, we provide evidence not only that the yield of cell-free HIV-1 particles is significantly reduced by BST-2 but also that the infectivity of these progeny virions is severely impaired. The lowered virion infectivity is due to the accumulation of pr55 Gag precursor and the p40Gag intermediates as well as to the loss of a mature core in the majority of HIV-1 particles. These data suggest that, in addition to impeding the release of HIV-1 particles from host cells, BST-2 may also interfere with the activation of viral protease and, as a result, impairs viral Gag processing as well as maturation of HIV-1 particles.
Refolding dynamics of stretched biopolymers upon force quench

Single-molecule force spectroscopy methods can be used to generate folding trajectories of biopolymers from arbitrary regions of the folding landscape. We illustrate the complexity of the folding kinetics and generic aspects of the collapse of RNA and proteins upon force quench by using simulations of an RNA hairpin and theory based on the de Gennes model for homopolymer collapse. The folding time, τF, depends asymmetrically on δfS = f S − f m and δf Q = f m − f Q where f S (f Q) is the stretch (quench) force and f m is the transition midforce of the RNA hairpin. In accord with experiments, the relaxation kinetics of the molecular extension, R(t), occurs in three stages: A rapid initial decrease in the extension is followed by a plateau and finally, an abrupt reduction in R(t) occurs as the native state is approached. The duration of the plateau increases as λ = τ Q/τ F decreases (where τ Q is the time in which the force is reduced from f S to f Q). Variations in the mechanisms of force-quench relaxation as λ is altered are reflected in the experimentally measurable time-dependent entropy, which is computed directly from the folding trajectories. An analytical solution of the de Gennes model under tension reproduces the multistage stage kinetics in R(t). The prediction that the initial stages of collapse should also be a generic feature of polymers is validated by simulation of the kinetics of toroid (globule) formation in semiflexible (flexible) homopolymers in poor solvents upon quenching the force from a fully stretched state. Our findings give a unified explanation for multiple disparate experimental observations of protein folding.
"All of a sudden we had this dialogue...": intercultural computer clubs' contribution to sustainable integration

A sustainable integration of migrants is an important societal task, touching numerous parts of everyday life. Developed as a socio-technical concept, intercultural computer clubs following the 'come_IN' approach are apt to provide a major contribution here. The aim is twofold: via collaboration in computer-based project work in the clubs, its participants a) establish and strengthen relationships in the intercultural neighborhood they now live in, and b) acquire computer skills that may broaden and ease their access to the job market as well as help them to keep up a remote relationship with their respective home countries. A qualitative evaluation study reveals the chances and limitations of this concept.
The effects of electroshock on immune function and disease progression in juvenile spring chinook salmon (Oncorhynchus tshawytscha)

Abstract Although much is known about the effects of electroshock on fish physiology, consequences to the immune system and disease progression have not received attention. Our objectives were to determine the effects of electroshock on selected immune function in juvenile spring chinook salmon Oncorhynchus tshawytscha, the mechanism of any observed alteration, and the effects of electroshock on disease progression. We found that the ability of anterior kidney leukocytes to generate antibody-producing cells (APC) was suppressed 3 h after a pulsed-DC electroshock (300 V, 50 Hz, 8 ms pulse width) but recovered within 24 h. This response was similar in timing and magnitude to that of fish subjected to an acute handling stress. The mechanism of suppression is hypothesized to be via an elevation of plasma cortisol concentrations in response to stress. Other monitored immune functions, skin mucous lysozyme levels, and respiratory burst activity were not affected by exposure to electroshock. The progression of a.
KIDSCREEN -52: PARENT'S PERCEPTION OF THEIR CHILDREN'S QUALITY OF LIFE

The KIDSCREEN 52 is a European cross-cultural and standardized instrument that assesses ten health related quality of life (HRQoL) dimensions in children, adolescents and their parents. It is often claimed that children self reports are not totally accurate, calling for a confirmation from parents or caregivers. The present study focuses only on the Kidscreen-52, parents' version (a parallel version addressed specifically to parents perceptions of their son's HRQoL). 2255 parents of children and adolescents with a mean age of 13.2, attending the 5 th grade and 7 th grade were inquired. The fit indexes indicate good fit to the data. Specifically, the final solution of the RMSEA was lower than . 03, the upper limit of 90% confidence interval was lower than . 05, and CFI was higher than . 95. Results indicate that the current 52 items structure is invariant across gender and age group. The parent's version of the Kidscreen-52 questionnaire constitutes a valid instrument to estimate parent's perception of quality of life in their
Increased expression of lncRNA HULC indicates a poor prognosis and promotes cell metastasis in osteosarcoma.

INTRODUCTION Long non-coding RNAs (lncRNAs) are aberrantly expressed in many diseases including cancer. LncRNA HULC (highly up-regulated in liver cancer) has recently been revealed to be involved in hepatocellular carcinoma development and progression. However, the role and function of HULC in human osteosarcoma remains unknown. METHODS LncRNA HULC expression in osteosarcoma tissues and cell lines was detected by quantitative real-time PCR. Then, the association of HULC level with survival of osteosarcoma patients was performed by the Kaplan-Meier and Cox proportional regression analyses. Furthermore, the effects of HULC on tumorigenicity of osteosarcoma cells were evaluated by in vitro assays. RESULTS In the present study, we demonstrated that HULC was significantly up-regulated in osteosarcoma tissues and cell lines compared with normal controls, and over-expression of HULC was correlated with clinical stage and distant metastasis. Moreover, higher HULC expression was associated with shorter overall survival of osteosarcoma patients. Furthermore, decreased expression of HULC markedly suppressed osteosarcoma cell proliferation, migration, and invasion. CONCLUSIONS Our results indicated that HULC is a novel molecule involved in osteosarcoma progression, which may provide a new marker of poor prognosis and a potential therapeutic target for osteosarcoma intervention.
Discourses of education and constitutions of class: public discourses on education in Swedish PBS television

Drawing on post-structural perspectives and analysis of television programs on education, the article investigates the public educational discourse in Sweden. It shows how a dominant neoliberal educational discourse is articulated together with a discourse of equal education, where the two discourses influence and subvert each other so that neither becomes totally hegemonic. Taking as its point of departure the neoliberal emphasis on the individual, especially as it relates to school choice and to the significance of class for educational success, the analysis focuses on the constitution of classed positions. The study reveals constitutions of class in which race, place, gender, economy and agency are intertwined, such that the schools and the students are attributed both different statuses and different subject positions in terms of future economic trajectories. The conclusions drawn are that, in the public conversation about the organization and goal of compulsory education, it is important to be aware of the discursive and political contexts in which the discussions take place. It is also important to realize that class matters in the educational assemblage in the form of economic subjectivities constituted in a web of intersecting notions about differing preconditions and outcomes of education.
On the performance of space-time codes over spatially correlated Rayleigh fading channels

We derive the exact pairwise error probability (PEP) for space-time coding over quasi-static Rayleigh fading channels in the presence of spatial fading correlation. We show that receive correlation always degrades the PEP for all signal-to-noise ratios (SNRs). We quantify the effect of receive correlation by employing the notion of "majorization". We show that the stronger the receive correlation, the worse the PEP for all SNRs. We show that at low SNR, depending on the codes, transmit correlation can either improve or degrade the PEP performance. We show that to guarantee robust performance for arbitrary transmit correlation, the minimum eigenvalue of the codeword pair difference matrix should be maximized among all codeword pairs.
Isolation of Serpulina pilosicoli from Rectal Biopsy Specimens Showing Evidence of Intestinal Spirochetosis

ABSTRACT Histologic evidence of intestinal spirochetosis (IS) was found in 22 of 41 (53.7%) rectal biopsy specimens from homosexual men attending a sexually transmitted diseases clinic. Serpulina pilosicoli was cultured from 11 of the IS-positive biopsy specimens (50%) and from 2 specimens (10.5%) in which spirochetes were not observed. The association between seeing spirochetes in biopsy specimens and isolating S. pilosicoli was statistically significant, clearly indicating that this spirochete is the agent of IS.
Self-adapting maxflow routing algorithm for WSNs: practical issues and simulation-based assessment

Autonomous wireless sensor networks are subject to power, bandwidth, and resource limitations that can be represented as capacity constraints imposed to their equivalent flow networks. The maximum sustainable workload of a sensor net work (i.e., the maximum data flow from the sensor nodes to the collection point which is compatible with the capacity constraints) is the maxflow of the flow network. This paper presents a self-adapting maxflow routing algorithm which is able to route any sustainable workload while automatically adapting to time-varying operating conditions. The algorithm has been implemented on top of OMNeT++ [1] in order to address practical issues and to enable simulation-based assessment and design exploration Simulation results demonstrate the effectiveness and the applicability of the proposed approach
Evaluation of a model for total body protein mass based on dual-energy X-ray absorptiometry: comparison with a reference four-component model.

The aim of the present study was to evaluate a model of body composition for assessing total body protein (TBP) mass using dual-energy X-ray absorptiometry (DXA), with either measured or assumed total body water (TBW); it was intended to provide a less complex or demanding alternative technique to, for example, the four-component model (4-CM). The following measurements were obtained in healthy adults (n 46) aged 18--62 years, and children (n 30) aged 8--12 years: body weight (BWt), body volume (BV; under-water weighing), TBW ((2)H-dilution space or predicted using an assumed hydration fraction of fat-free mass (HF(ffm))), bone mineral content (BMC; DXA) and fat-free soft tissue (FFST; DXA). TBP was calculated using the 4-CM (TBP = 3.05BWt -- 0.290TBW -- 2.734BMC -- 2.74BV) and the DXA model (TBP = FFST -- 0.2302BMC -- TBW). DXA measurements were obtained using the Lunar DPX (Lunar Radiation Corporation, Madison, WI, USA) or Hologic QDR 1000/W (Hologic, Waltham, MA, USA). Precision of the DXA model for TBP with measured TBW (4.6--6.8 % mean TBP) was slightly worse than the 4-CM (4.0--5.4 %), whereas that modelled with assumed HF(ffm) was more precise (2.4--5.2 %) because it obviated imprecision associated with measuring TBW. Agreement between the 4-CM and DXA model with measured TBW was also worse (e.g. bias, 15 % of the mean; 95 % limits of agreement up to +/-39 % for adults measured on the Lunar DPX) than when a constant for HF(ffm) was assumed (3.7 % and +/-21 % respectively). Most of the variability in agreement between these various models was due to interpretation of biological factors, rather than to measurement imprecision. Therefore, the DXA model, which is less complex and demanding than the 4-CM, is of value for assessing TBP in groups of healthy subjects, but is of less value for individuals in whom there may be substantial differences from reference 4-CM estimates.
Fibroblast growth factor reversal of the corneal myofibroblast phenotype.

PURPOSE Keratocytes give rise to fibroblasts and myofibroblasts in wounded cornea. It is well established that treatment of fibroblasts with transforming growth factor (TGF) beta will induce myofibroblast differentiation. We investigated whether this differentiation could be reversed by the administration of fibroblast growth factor (FGF). METHODS Cultured corneal myofibroblasts were plated at 200 cells/mm(2), and cells were grown in DMEM/F12 containing (1) 10% FBS or (2) 10% FBS with FGF and heparin or (3) 1% FBS or (4) 1% FBS with TGF-beta. As distinguished from the fibroblast phenotype, the myofibroblast phenotype was identified by the assembly of alpha-smooth muscle (SM) actin protein into the stress fiber cytoskeleton. To further characterize growth factor regulation of the two phenotypes, the phenotypic expression of TGF-beta receptor types I and II, cadherins, and connexin 43 by immunocytochemistry, Western blot analysis, and immunoprecipitation and of alpha-SM actin mRNA in Northern blot analysis were evaluated. RESULTS Corneal myofibroblasts replated and grown in the presence of FGF-1 or FGF-2 (20 ng/ml) plus heparin (5 microg/ml) in 10% FBS medium had decreased expression of alpha-SM actin protein, TGF-beta receptors, and cadherins. Thus, FGF-heparin decreased the myofibroblast phenotype and promoted the fibroblast phenotype. Administration of either 20 ng/ml FGF or 5 microg/ml heparin alone was not effective. Addition of TGF-beta further enhanced the expression of alpha-SM actin mRNA and protein and cell surface expression of TGF-beta receptors in myofibroblast cultures. CONCLUSIONS FGF-1 or -2 and heparin promoted the fibroblast phenotype and reversed the myofibroblast phenotype. This finding supports the idea that corneal myofibroblasts and fibroblasts are alternative phenotypes rather than terminally differentiated cell types.
The learning behavior of single neuron classifiers on linearly separable or nonseparable input

Determining linear separability is an important way of understanding structures present in data. We explore the behavior of several classical descent procedures for determining linear separability and training linear classifiers in the presence of linearly nonseparable input. We compare the adaptive procedures to linear programming methods using many pairwise discrimination problems from a public database. We found that the adaptive procedures have serious implementation problems which make them less preferable than linear programming.
A Novel Hardware-Based All-Digital Phase-Locked Loop Applied to Grid-Connected Power Converters

For grid-connected power converters, the frequency and phase angle of the grid voltage, which are essential to the system operations, must be quickly and accurately obtained even if the utility voltage is distorted or unbalanced. In this paper, a novel hardware-based all-digital phase-locked loop (ADPLL) is proposed for grid interface converters to detect the frequency and phase angle based on the voltage zero crossings. The proposed ADPLL features wide track-in range and fast pull-in time, and it can easily be integrated with the digital controller for grid-connected power converters. A discrete small-signal model is presented to investigate the performance and parameter dependence of the ADPLL. As expected, the output phase error and pulse jitter are minimized by selecting a high clock frequency and proper regulator parameters. With additional voltage sensors, the ADPLL can be readily extended into applications with grid disturbances. Experimental results verify the analysis and the effectiveness of the ADPLL.
Enzyme characteristics of beta-D-galactosidase- and beta-D-glucuronidase-positive bacteria and their interference in rapid methods for detection of waterborne coliforms and Escherichia coli.

Bacteria which were beta-D-galactosidase and beta-D-glucuronidase positive or expressed only one of these enzymes were isolated from environmental water samples. The enzymatic activity of these bacteria was measured in 25-min assays by using the fluorogenic substrates 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide. The enzyme activity, enzyme induction, and enzyme temperature characteristics of target and nontarget bacteria in assays aimed at detecting coliform bacteria and Escherichia coli were investigated. The potential interference of false-positive bacteria was evaluated. Several of the beta-D-galactosidase-positive nontarget bacteria but none of the beta-D-glucuronidase-positive nontarget bacteria contained unstable enzyme at 44.5 degrees C. The activity of target bacteria was highly inducible. Nontarget bacteria were induced much less or were not induced by the inducers used. The results revealed large variations in the enzyme levels of different beta-D-galactosidase- and beta-D-glucuronidase-positive bacteria. The induced and noninduced beta-D-glucuronidase activities of Bacillus spp. and Aerococcus viridans were approximately the same as the activities of induced E. coli. Except for some isolates identified as Aeromonas spp. , all of the induced and noninduced beta-D-galactosidase-positive, noncoliform isolates exhibited at least 2 log units less mean beta-D-galactosidase activity than induced E. coli. The noncoliform bacteria must be present in correspondingly higher concentrations than those of target bacteria to interfere in the rapid assay for detection of coliform bacteria.