Miracle-dz/UniFAR-FTUs huggingface dataset:
Copurification of glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase activities of Escherichia coli: characterization of the glmU gene product as a bifunctional enzyme catalyzing two subsequent steps in the pathway for UDP-N-acetylglucosamine synthesis

The glmU gene product of Escherichia coli was recently identified as the N-acetylglucosamine-1-phosphate uridyltransferase activity which catalyzes the formation of UDP-N-acetylglucosamine, an essential precursor for cell wall peptidoglycan and lipopolysaccharide biosyntheses (D. Mengin-Lecreulx and J. van Heijenoort, J. Bacteriol. 175:6150-6157, 1993). Evidence that the purified GlmU protein is in fact a bifunctional enzyme which also catalyzes acetylation of glucosamine-1-phosphate, the preceding step in the same pathway, is now provided. Kinetic parameters of both reactions were investigated, indicating in particular that the acetyltransferase activity of the enzyme is fivefold higher than its uridyltransferase activity. In contrast to the uridyltransferase activity, which is quite stable and insensitive to thiol reagents, the acetyltransferase activity was rapidly lost when the enzyme was stored in the absence of reducing thiols or acetyl coenzyme A or was treated with thiol-alkylating agents, suggesting the presence of at least one essential cysteine residue in or near the active site. The acetyltransferase activity is greatly inhibited by its reaction product N-acetylglucosamine-1-phosphate and, interestingly, also by UDP-N-acetylmuramic acid, which is one of the first precursors specific for the peptidoglycan pathway. The detection in crude cell extracts of a phosphoglucosamine mutase activity finally confirms that the route from glucosamine-6-phosphate to UDP-N-acetylglucosamine occurs via glucosamine-1-phosphate in bacteria.
Sistem informasi biaya balik nama kendaraan bermotor melalui aplikasi sms gateway

Tujuan dari penelitian ini adalah untuk meningkatkan pelayanan publik dan efektifitas kinerja di kantor SAMSAT Kota Padang dengan cara memberikan informasi biaya BBNKB kepada masyarakat yang akan melakukan transaksi balik nama. Biaya Balik Nama Kendaraan Bermotor (BBNKB) termasuk salah satu transaksi yang paling banyak dilakukan pada kantor SAMSAT Padang. Saat ini media pendukung untuk biaya balik nama kendaraan belum ada, sehingga orang wajib pajak tidak mengetahui berapa biaya yang harus mereka bayar. Untuk menyelesaikan masalah tersebut perlu dikembangkan sebuah Sistem Informasi Biaya Balik Nama Kendaraan Bermotor (BBNKB) berbasis SMS Gateway. Pengembangan software berbasis SDLC dengan metode XP (Extreme Programming). Sistem ini dikembangkan dengan bahasa pemrograman VB.Net, dan database MySQL sebagai menyimpan data serta Gammu sebagai konfigurasi SMS Gateway. Hasil dari penelitian ini adalah sebuah aplikasi yang dapat digunakan bagi wajib pajak agar mengetahui jumlah biaya balik nama kendaraan bermotor, yang dapat meningkatkan efesiensi kerja pada kantor SAMSAT kota Padang. Kata kunci: SMS Gateway, SAMSAT, Extreme Programming Abstract The purpose of this research is to improve public services and effectiveness of performance at the SAMSAT office in the city of Padang by providing information on BBNKB costs to people who will conduct transactions behind the name. The cost of transferring motor vehicle names (BBNKB) is one of the most widely carried out transactions at the SAMSAT Padang office. At present there are no supporting media for the return of vehicle names, so that taxpayers do not know how much they must pay. To solve this problem, it is necessary to develop an Motorbike Name Return Fee Information System (BBNKB) based on SMS Gateway. SDLC based software development with XP (Extreme Programming) method. This system was developed with the VB.Net programming language, and the MySQL database as a data store and Gammu as an SMS Gateway configuration. The results of this study are an application that can be used for taxpayers to find out the amount of return costs for motorized vehicles, which can improve work efficiency at the SAMSAT office in the city of Padang. Keywords: SMS Gateway, SAMSAT, Extreme Programming
A General Formula of the Capacity Region for Multiple-Access Channels with Deterministic Feedback

SUMMARY The multiple-access channel (MAC) becomes very popular in various communication systems, because multi-terminal communication systems have been widely used in practical systems, e.g., mobile phones and P2P, etc. For some MACs, it is known that feedback can enlarge the capacity region, where the capacity region is the set of rate pairs such that the error probability can be made arbitrarily small for sufﬁciently large block length. The capacity region for general MACs, which are not required to satisfy ergodicity and stationarity with perfect feedback was ﬁrst shown by Tatikonda and Mitter without the proof, where perfect feed-back means that the channel output is perfectly fed back to senders. In this paper, we generalize Tatikonda and Mitter’s result to the case of deterministic feedback, where the values of deterministic functions of past channel outputs is fed back to senders. We show that the capacity region for general MACs with deterministic feedback can be represented by the information-spectrum formula introduced by Han and Verd´u, and directed information introduced by Massey. We also investigate the compound MAC problem, the ε -coding problem, the strong converse property and the cost constraint problem for general MACs with deterministic feedback.
The Role of Opaque2 in the Control of Lysine-Degrading Activities in Developing Maize Endosperm

We have isolated a cDNA clone, designated ZLKRSDH, encoding the bifunctional enzyme lysine–ketoglutarate reductase/saccharopine dehydrogenase (LKR/SDH) from maize. The predicted polypeptide has an N-terminal LKR domain and a C-terminal SDH domain that are similar to the yeast LYS1 and LYS9 monofunctional proteins, respectively. The maize LKR/SDH protein is located in the cytoplasm of subaleurone endosperm cell layers. Transcripts and polypeptides as well as enzyme activities showed an upregulation and downregulation during endosperm development. The developmental expression of ZLKRSDH was examined in normal and opaque2 seeds. In the mutant endosperm, mRNA levels were reduced by >90%, with concomitant reductions in polypeptide levels and LKR/SDH activity. These results suggest that lysine levels in the endosperm are likely to be controlled at the transcriptional level by the Opaque2 transcription factor.
Upgrading of Symbolic and Synthetic Knowledge Bases: Evidence from the Chinese Automotive and Construction Industries

Abstract This paper deals with the question of how upgrading of the symbolic and synthetic knowledge bases takes place and, by doing so, we contribute to the upgrading literature by linking upgrading with the concept of the differentiated knowledge bases. We discern a number of upgrading mechanisms, and based on empirical evidence from the construction industry and the automotive industry in China, we show that the main upgrading mechanisms for symbolic knowledge include “learning-by-interacting in project teams” and “monitoring”, while upgrading of synthetic knowledge takes place via “technology transfer” and “learning-by-doing and-using”. “Mobility” and “on-the-job training and learning in Transnational Corporations” are the main upgrading mechanisms contributing to the development of both knowledge bases.
Effect of Lures and Colors on Capture of Lady Beetles (Coleoptera: Coccinellidae) in Tedders Pyramidal Traps

ABSTRACT Purposeful attraction and aggregation of adult Coccinellidae at target sites would be useful for sampling purposes and pest suppression. We field-tested 1) lures in yellow and black pyramidal traps and 2) pyramidal traps that had been painted one or two colors (without lures) to determine if lures or trap color affected capture of adult Coccinellidae. In only one experiment with lures did a single rate of limonene increase trap capture, whereas no other lure ever did. Yellow traps, regardless of using a lure, always captured significantly more lady beetles than black traps. When single-color red, orange, yellow, green, blue, purple, black, and white traps (without lures) were tested, yellow traps captured significantly more lady beetles. Of all species of Coccinellidae captured in these single-color traps, 95% were the exotic species Harmonia axyridis (Pallas) and Coccinella septempunctata L. H. axyridis alone dominated trap capture comprising 74.1% of all lady beetles. Two-color traps (yellow—green, yellow—orange, yellow—white, and yellow—black) never captured more than single-color yellow traps. These results demonstrate that yellow pyramidal traps can be used to purposefully attract, and when used without a collection device, possibly aggregate adult Coccinellidae at targeted field sites.
Breast preservation in stage I and II carcinoma of the breast. The University of Chicago experience.

BACKGROUND Although breast conservation has received increased acceptance, there are still unresolved issues regarding local treatment techniques, such as the extent of surgery, in relation to the final margins of excision and the use of tumor bed boost radiation. The goal of this study was to determine the local control and breast preservation with particular emphasis on the importance of the final microscopic margins in patients receiving tumor bed boost therapy. METHODS The authors analyzed 869 cases of Stage I and II breast carcinoma in 852 women who were treated with breast-conserving surgery and radiation therapy between 1984 and 1994. The median follow-up was 43 months. Final microscopic margins were negative in 762 (88%), microscopically positive in 82 (9%), and unknown in 25 (3%) of the patients. Negative margins were defined as no tumor cells at the surgical margin. The patients were treated with external beam radiation therapy to the entire breast to a median dose of 46 Gray (Gy). A boost to the tumor bed was delivered to 863 (99%) of the patients. The median tumor bed dose was 60 Gy. A multivariate analysis of factors impacting on the local control and overall survival was performed. Variables introduced into the model included size, age, lymph node status, microscopic margins, nuclear grade, histologic grade, and estrogen and progesterone receptor status. RESULTS The actuarial 5-year local control rate was 97%. The median time to local failure was 32 months (range, 14-69 months). In multivariate analysis, the only significant factor affecting local control was the status of margins. In patients receiving boost radiation to the excision site, the local control rate at 5 years was 98% and 89%, respectively, if the margins were negative or positive (P < 0.01). This resulted in 5-year actuarial breast preservation rates of 98% and 92% (P = 0.03). In the patients in whom the margins of excision were microscopically positive, the local control rate was 91% if the total dose to the tumor bed was > 60 Gy compared with 76% for a dose < or = 60 Gy (P = 0.05). The 5-year actuarial overall survival rate was 89%. Approximately 94% of the women considered their cosmetic outcome good to excellent. CONCLUSIONS By obtaining microscopically negative margins and using tumor bed boost therapy, excellent local control, breast preservation, and cosmesis can be achieved. In patients with microscopically positive margins, an acceptable local control rate can be achieved if a tumor bed boost is given.
Iron Loss Analysis of the Permanent-Magnet Synchronous Machine Based on Finite-Element Analysis Over the Electrical Vehicle Drive Cycle

Fast methods to estimate iron losses of the permanent magnet traction motor during the drive cycle of the electrical vehicle are compared. The methods use the iron loss information calculated by a finite-element analysis as a function of rotational speed both at no load and with a short-circuited stator to take into account the variable frequency and field weakening of the traction motor. The effect of iron losses on the optimal current components, providing the maximum efficiency, is studied. Several methods yield a good accuracy even based on the no-load iron loss only, but the accuracy can be improved especially in deep field weakening by including the short-circuit iron loss information in the analysis.
Changing Seasonal Predictability of Arctic Summer Sea Ice Area in a Warming Climate

We use a large ensemble set of simulations and initialized model forecasts to assess changes in the initial-value seasonal predictability of summer Arctic sea ice area from the late-twentieth to the mid-twenty-first century. Ice thickness is an important seasonal predictor of September ice area because early summer thickness anomalies affect how much melt out occurs. We find that the role of this predictor changes in a warming climate, leading to decadal changes in September ice area predictability. In January-initialized prediction experiments, initialization errors grow over time leading to forecast errors in ice thickness at the beginning of the melt season. The magnitude of this ice thickness forecast error growth for regions important to summer melt out decreases in a warming climate, contributing to enhanced predictability. On the other hand, the influence of early summer thickness anomalies on summer melt out and resulting September ice area increases as the climate warms. Given this, for the same magnitude ice thickness forecast error in early summer, a larger September ice area anomaly results in the warming climate, contributing to reduced predictability. The net result of these competing factors is that a sweet spot for predictability exists when the ice thickness forecast error growth is modest and the influence of these errors on melt out is modest. This occurs at about 2010 in our simulations. The predictability of summer ice area is lower for earlier decades, because of higher ice thickness forecast error growth, and for later decades because of a stronger influence of ice thickness forecast errors on summer melt out.
Is Self-determination Contagious? A Spatial Analysis of the Spread of Self-Determination Claims

Abstract Self-determination claims have abounded in the international system since the end of World War II. But these claims have not emerged everywhere. About half of the states in the international system face some challenge related to self-determination today. Why do some states face these demands while others do not? We argue that ethno-national self-determination is one of many identities with which individuals can find affinity. While an international norm related to self-determination has developed globally, its use as a basis for political claims has diffused regionally. Diffusion of self-determination occurs through observation of others using self-determination as a basis of organization, generating a sense of legitimacy, sensitivity to related grievance, and perceptions of tangible benefits related to self-determination identification. We test this empirically on global data on self-determination claims from 1960 to 2005 and find evidence of spatial diffusion, suggesting that self-determination is, to some extent, contagious.
Copy Move Image Forgery Detection using Pseudo Zernike Moment for Better Detection Accuracy

In modern era of information age, digitalization has revolutionized like never before. Powerful computers, advanced photo editing software packages and high resolution capturing devices have made manipulation of digital images incredibly easy. As per as image forensics concerns, one of the most actively researched area are detection of copy move forgeries. Higher computational complexity is one of the major component of existing techniques to detect such tampering. Moreover, copy move forgery is usually performed in three steps. First, copying of a region in an image then pasting the same one in the same respective image and finally doing some post-processing like rotation, scaling, shift, noise, etc. Consequently, pseudo Zernike moment is used as a features extraction method for matching image blocks and as a primary factors on which performance of detection algorithms depends.
Distribution of cerebral blood flow in the circle of Willis.

PURPOSE To prospectively determine the effect of anatomic variations in the circle of Willis on volume flow in the internal carotid arteries (ICAs) and basilar artery (BA). MATERIALS AND METHODS Institutional review board approval and informed consent were obtained. Phase-contrast magnetic resonance (MR) angiography was used to measure the volume flow in the BA and ICAs in 208 patients (182 men, 26 women; mean age, 60 years) with symptomatic atherosclerosis or risk factors for atherosclerosis. Patients with steno-occlusive disease were excluded, and flow values were normalized for age. Three-dimensional time-of-flight MR angiograms were used to assess the anatomy of the circle of Willis. Differences in volume flow between a complete circle of Willis, a circle with a missing A1 segment, and a circle with a fetal-type posterior cerebral artery were analyzed (analysis of variance and Scheffe post hoc tests). RESULTS The ICA volume flow in subjects with a complete configuration of the circle of Willis was 245 mL/min +/- 65 (standard deviation). Flow in the contralateral ICA was significantly increased (P < .01) in subjects with a missing A1 segment (303 mL/min +/- 56) compared with control subjects and compared with flow on the ipsilateral side (214 mL/min +/- 94; P < .01). In subjects with a unilateral or bilateral fetal-type posterior cerebral artery, the ICA volume flow was increased (P < .01) and the BA volume flow was decreased (P < .01) in comparison with the flow in subjects with no fetal-type circle of Willis. CONCLUSION Large asymmetries in volume flow between the right and left ICAs or decreased volume flow in the BA is not necessarily caused by vascular disease but may be caused by variations in the anatomy of the circle of Willis.
Routing algorithm for multi-FPGA based systems using multi-point physical tracks

Multi-FPGA boards suffer from large timing delays in inter-FPGA physical tracks compared to intra-FPGA track delays, as well as a limited bandwidth between FPGAs due to the limited number of I/Os per FPGA. In order to tackle this problem, an algorithm which routes multi-terminal nets in multi-point tracks is proposed in this paper to spare FPGA I/Os. Experiments are conducted using Gaisler Research Benchmarks. Firstly, each testbench will be implemented in an off-the-shelf board. The results show that the system frequency can be increased in the off-the-shelf board by the proposed routing algorithm. Secondly, an automatic design flow which generates a custom multi-FPGA board is enhanced by generating multi-point tracks in the board, and each testbench will be implemented with the proposed routing algorithm in custom boards. The results show that the system frequency is improved in the custom board with both 2- and multi-point tracks.
Bone histology of Azendohsaurus laaroussii: Implications for the evolution of thermometabolism in Archosauromorpha

Abstract This paper is aimed at constraining the phylogenetic frame of the acquisition of endothermy by Archosauromorpha. We analyzed the bone histology of Azendohsaurus laaroussii. Stylopodial and zeugopodial bones show three tissue types: (1) avascular lamellar zonal bone formed at low growth rates; (2) a scaffold of parallel-fibered bone containing either small primary osteons or simple vascular canals; and (3) fibrolamellar bone formed at high growth rates. We used quantitative histology to infer the thermometabolic regime of this taxon. We define endothermy as the presence of any mechanism of nonshivering thermogenesis that increases both body temperature and resting metabolic rate. Thus, estimating the resting metabolic rate of an extinct organism may be a good proxy to infer its thermometabolic regime (endothermy vs. ectothermy). High resting metabolic rates have been shown to be primitive for the clade Prolacerta–Archosauriformes. Therefore, we inferred the resting metabolic rates of A. laaroussii, a sister group of this clade, and of 14 extinct related taxa, using phylogenetic eigenvector maps. All the inferences obtained are included in the range of variation of resting metabolic rates measured in mammals and birds, so we can reasonably assume that all these taxa (including Azendohsaurus) were endotherms. A parsimony optimization of the presence of endothermy on a phylogenetic tree of tetrapods shows that this derived character state was acquired by the last common ancestor of the clade Azendohsaurus–Archosauriformes and that there is a reversion in Crocodylia.
Mouse Cumulus-Oocyte Complexes from In Vitro-Cultured Preantral Follicles Suggest an Anti-Luteinizing Role for the EGF Cascade in the Cumulus Cells1

The mammalian ovulatory process is a fairly complex succession of events that leads to the release of a competent oocyte. The luteinizing hormone (LH) triggers the cascade of events, which starts with the production of secondary messengers in the follicular wall and ends with the release of a fertilizable oocyte. Most of these events can be reproduced using in vitro models, which offer a wide range of possibilities for study strategies. Although it is accepted that epidermal growth factor receptor (EGFR) activation is required for transmission of the LH-initiated signal, we hypothesized that LH receptor activation might also play a role in oocyte meiotic resumption and cumulus cell response, because the current mouse preantral follicle in vitro model expresses functional LH receptor. To separate the LH-mediated response and the epidermal growth factor (EGF)-mediated response (following LH stimulus), in vitro-grown mouse ovarian follicles were stimulated for ovulation with a combination of human chorionic gonadotropin (hCG) plus galardin (inhibitor for the release of endogenous EGF-like factors) or hCG plus galardin plus EGF. Results suggest that the stimulation provided by LH (hCG) is insufficient to induce a maximum oocyte meiotic resumption and that EGFR activation is also required. Analysis of transcript levels of Egfr, Ereg, Cyp19a1, Hsd3b1, Adamts1, and Has2 in cumulus cells further indicate that the triggers for the EGFR cascade preserve the expression profile of the studied transcripts. Therefore, it is proposed that within this in vitro mouse model, EGF signaling during ovulation might protect the cumulus cells from the potential luteinizing effects of LH.