Miracle-dz/UniFAR-FTUs huggingface dataset:
Endothelial inclusions and "nuclear bodies" in systemic lupus erythematosus.

In recent years many reports on special cytoplasmic inclusions in cells of involved tissues of various organs in a number of 'autoimmune' diseases have been published. Most frequently, these inclusions are demonstrated in the endothelial cells of blood vessels, and are therefore often called 'endothelial inclusions' (El), though they are occasionally found in fibroblasts, pericytes, macrophages, lymphocytes, and plasma cells. In systemic lupus erythematosus (SLE), EI have been described in the kidney (Fresco, 1968; Gyorkey, Min, Sincovics, and Gyorkey, 1969; Gyorkey and Gyorkey, 1971; Kawano, Miller, and Kimmelstiel, 1969; Hurd, Eigenbrodt, Ziff, and Strunk, 1969; Hurd, Eigenbrodt, Strunk, and Ziff, 1970; Sinkovics, Gyorkey, and Thoma, 1969; Pincus, Blacklow, Grimley, and Bellanti, 1970; Grausz, Earley, Stephens, Lee, and Hopper, 1970; Helder, Blomjous, Feltkamp-Vroom, and van Loghem, 1971), skeletal muscle (Norton, 1969), skin (Hashimoto, 1969; Helder and others 1971), and synovial tissue (Schumacher, 1970; Dreyer, Muldiyarov, Nassonova, and Alekberova, 1973). It has been assumed that El are related to the possible aetiologic factor of SLE and that their tubular structures are either of virus origin or appear as a result of virus infection. The majority of investigators believe that these tubular structures closely resemble the nucleoprotein strands of paramyxoviruses. Recently, Neumark and Farkas (1970) described so-called 'nuclear bodies' (NB) in rheumatoid (RA) synovial tissue and suggested that these inclusions were also of virus origin. These authors found NB in endothelial cells, pericytes, and lining cells of 22 patients, but failed to find them in control subjects. We studied biopsies of synovial tissue, kidney, and skin in definite SLE and found both El and NB in the same tissue samples. The NB in SLE proved to be even more characteristic of the affected synovial tissue than the El. Material and methods Five synovial biopsies, three skin biopsies, and two kidney biopsies from nine patients with definite SLE were studied with the electron microscope. The skin biopsies were taken only from uninvolved areas. A brief case history and laboratory data of each patient are summarized in the Table. The specimens were fixed for 1 to 2 hrs in ice-cold 2-5 per cent. glutaraldehyde, buffered to pH 7-4 with phosphate buffer, washed for 30 min. in phosphate buffer, post-fixed in cold Caulfield's fixative for 2 hrs, dehydrated with ethanol, and embedded in araldite. The tissue blocks were stained with 1 per cent. uranyl acetate in 70 per cent. ethanol during dehydration. Ultrathin sections were prepared on an LKB III ultramicrotome, stained with lead citrate (Reynolds, 1963), and examined in a JEM-7 electron microscope. Sections 2 to 4 pum. thick were made of all the tissue blocks embedded for electron microscopy, stained according to Richardson, Jarett, and Finke (1960), and examined in the light microscope for orientation.
Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components

ABSTRACT The Smc5/6 complex in Saccharomyces cerevisiae contains six essential non-Smc elements, Nse1-6. With the exception of Nse2 (also known as Mms21), which is an E3 small ubiquitin-like modifier (SUMO) ligase, very little is understood about the role of these components or their contribution to Smc5/6 functionality. Our characterization of Nse5 establishes a previously unidentified relationship between the Smc5/6 complex and factors of the SUMO pathway. Nse5 physically associates with the E2 conjugating enzyme, Ubc9, where contacts are stabilized by non-covalent interactions with SUMO. SUMO also mediates the interactions between Nse5 and the two PIAS family E3 SUMO ligases, Siz1 and Siz2. Cells carrying the nse5-ts1 allele or lacking either SIZ1 or SIZ2 exhibit a reduction in Smc5 sumoylation upon MMS treatment and demonstrate functional redundancy for SUMO mediated events in the presence of DNA damage. Overall, given the extensive connection between Nse5 and components of the SUMO pathway, we speculate that one function of the Smc5/6 complex might be as a scaffold center to enable sumoylation events in budding yeast. Summary: Here we characterize Nse5, a component of the Smc5/6 complex. Nse5 interacts with the PIAS E3 SUMO ligases, Siz1 and Siz2, and regulates Smc5 sumoylation.
Investigating the effect of high spring incidence of pandemic influenza A(H1N1) on early autumn incidence

SUMMARY A pandemic H1N1 infection wave in the USA occurred during spring 2009. Some hypothesized that for regions affected by the spring wave, an autumn outbreak would be less likely or delayed compared to unaffected regions because of herd immunity. We investigated this hypothesis using the Outpatient Influenza-like Illness (ILI) Network, a collaboration among the Centers for Disease Control and Prevention, health departments, and care providers. We evaluated the likelihood of high early autumn incidence given high spring incidence in core-based statistical areas (CBSAs). Using a surrogate incidence measure based on influenza-related illness ratios, we calculated the odds of high early autumn incidence given high spring incidence. CBSAs with high spring ILI ratios proved more likely than unaffected CBSAs to have high early autumn ratios, suggesting that elevated spring illness did not protect against early autumn increases. These novel methods are applicable to planning and studies involving other infectious diseases.
Combination of remote ischemic perconditioning and remote ischemic postconditioning fails to increase protection against myocardial ischemia/reperfusion injury, compared with either alone

Remote ischemic perconditioning (RIPerC) and remote ischemic postconditioning (RIPostC) have been previously demonstrated to protect the myocardium against ischemia/reperfusion (IR) injury. However, their combined effects remain to be fully elucidated. In order to investigate this, the present study used an in vivo rat model to assess whether synergistic effects are produced when RIPerC is combined with RIPostC. The rats were randomly assigned to the following groups: Sham, IR, RIPerC, RIPostC and RIPerC + RIPostC groups. The IR model was established by performing 40 min of left coronary artery occlusion, followed by 2 h of reperfusion. RIPerC and RIPostC were induced via four cycles of 5 min occlusion and 5 min reperfusion of the hindlimbs, either during or subsequent to myocardial ischemia. On measurement of infarct sizes, compared with the IR group (49.45±6.59%), the infarct sizes were significantly reduced in the RIPerC (34.36±5.87%) and RIPostC (36.04±6.16%) groups (P<0.05). However, no further reduction in infarct size was observed in the RIPerC + RIPostC group (31.43±5.43%; P>0.05), compared with the groups treated with either RIPerC or RIPostC alone. Activation of the reperfusion injury salvage kinase (RISK) Akt, extracellular signal-regulated kinase 1/2 and glycogen synthase kinase-3β, and survivor activating factor enhancement (SAFE) signal transducer and activator of transcription-3 pathways were enhanced in the RIPerC, RIPostC and the RIPerC + RIPostC groups, compared with the IR group, with no difference among the three groups. Therefore, whereas RIPerC and RIPostC were equally effective in providing protection against myocardial IR injury, the combination of RIPerC and RIPostC failed to provide further protection than treatment with either alone. The cardioprotective effects were found to be associated with increased activation of the RISK and SAFE pathways.
Proposing Enterprise Wide Information Systems Business Performance Model

Enterprise Wide Information Systems (EWIS) implementation involves the entire business and will require changes throughout the firm. Because of the scope, complexity and continuous nature of ERP, the project-based approach to managing the implementation process resulted in failure rates of between 60% and 80%. In recent years ERP systems have received much attention. The organizational relevance and risk of ERP projects make it important for organizations to focus on ways to make ERP implementation successful. Once these systems are in place, however, their performance depends on the identified macro variables viz. ‘Business Process’, ‘Decision Making’ and ‘Individual / Group working’. The questionnaire was designed and administered. The responses from 92 organizations were compiled. The relationship of these variables with EWIS performance is analyzed using inferential statistical measurements. The study helps to understand the performance of model presented. The study suggested in keeping away from the calamities and thereby giving the necessary competitive edge. Whenever some discrepancy is identified during the process of performance appraisal care has to be taken to draft necessary preventive measures. If all these measures are taken care off then the EWIS performance will definitely deliver the results. Keywords— Enterprise Systems, performance, technology
[Study on culture and in vitro osteogenesis of blood-derived human mesenchymal stem cells].

OBJECTIVE To establish a method of isolating and culturing adult human blood-derived stem cells(MSCs) and to investigate their osteogenic potential in vitro. METHODS Thirty peripheral blood collected from 30 adult volunteers (15 ml per person). Adult human MSCs derived from peripheral blood from the lymphocyte separation fluid fraction of mononuclear cells, cultured in alpha-Modified Eagle's Medium glucose containing 20% fetal bovine serum, and proliferated through a process of subculturing. The phenotype was analyzed with flow cytometry. For in vitro osteogenic differentiation, MSCs from the second passage presence of osteogenic supplements (100 nmol/L dexamethasone,10 mmol/L beta-glycerophosphate, 50 micromol/L and 10 nmol/L 1,25-2-hydroxide vitamin D3). In the fifth passage cells, the activity of alkaline phosphatase, expression level of collagen type I, osteocalcin and osteonectin were determined. And the calcium tubercle would be examined after the continual one-month culture of the fifth passage. RESULTS MSCs existed in blood of adult human. And the clone forming efficiency of blood-derived MSCs was 0.27 +/- 0.22/10(6) mononuclear The MSCs expressed CD44,CD54,CD105,and CD166, but did not CD14, CD34, CD45,and CD31. Under osteogenic supplements, the MSCs were found to be higher activity of alkaline phosphatase and higher expression of collagen type I , osteocalcin and osteonectin. And the calcium tubercle formation was examined through fluorescence labeling method. CONCLUSION The isolation and culture conditions established for adult human select a distinct population of peripheral blood-derived adherent cells. Adult human blood-derived osteogenic potential in vitro, and may be used as seed cells for bone tissue engineering.
Al 6061-TiB 2 Metal Matrix Composite Synthesized with Different Reaction Holding Times by In-Situ Method

Metal matrix composites (MMCs) have been developed to meet the requirements in the field of transportation and similar engineering applications due to their superior mechanical properties. Aluminium (Al) alloys are used as common matrix phases for the production of a variety of metal matrix composites due to their low density, good corrosion resistance, high electrical and thermal conductivities, high damping capacity and their ability to be strengthened by precipitation. Al 6061 alloys are widely used in many engineering applications in the fields of construction and transportation where superior mechanical properties such as hardness and tensile strength are essential. By incorporating TiB2 reinforcement phase in the Al 6061 alloy, Al 6061-TiB2 metal matrix composite can be manufactured using the in-situ salt –metal reaction technique. The addition of TiB2 to the metal matrix increases the strength of the matrix phase. An attempt was made to synthesize Al 6061 and TiB2 using in-situ technique where halide salts namely potassium hexaflurotitanate (K2TiF6) and potassium tetrafluroborate (KBF4) were introduced in the Al 6061 melt at 850°C with different reaction holding times in order to investigate the degree of in-situ reaction in the synthesis of Al 6061 – TiB2 metal matrix composite.
Characterisation and advanced applications of the steam oxidation of AlGaAs

The high temperature, wet oxidation of buried, high aluminium content AlGaAs layers has seen a lot of attention in recent years thanks to its ability to form good quality, deep and uniform oxide layers a unique attribute among the III-V materials. It is the unusual combination of relatively high density and good porosity of the oxide material, that allows the oxidation to reach much greater depths than that of other materials, and this is what makes the process so widely employable and versatile. This project is based on two systems of oxidisable layers, incorporated into two wafers, both of which were designed as part of the work. One wafer, referred to as Wafer A, contains 30nm 98% aluminium layers in both of the cladding regions, and the second, Wafer B, has spaced stacks of 1nm 95% aluminium layers placed directly into the active region. The oxidation depths of both wafers are mapped over a range of temperatures and times, and maximum measured rates of 2um/min and 1um/min are reported for Wafers A and B respectively. Further to this, a non-trivial dependence of the oxidation rate on dopant species is reported. It is found that although p-type material initially achieves greater oxidation rates, after a depth of approximately 8pm has been reached, the n-type material over takes. This result is contrary to the popular belief that p-type material oxidises faster, regardless of depth. The two wafers are then made into working devices. In the case of Wafer A, longitudinal oxidation of broad-area devices are used to create unpumped saturable absorber sections. These sections are responsible for various effects including the lowering of laser threshold currents by up to 30%, the narrowing of laser near-fields by up to 50%, and the production of repetitive wavelength shifting behaviour, which is investigated in great detail. Wafer B is oxidised laterally to produce current confinement directly in the active region. Threshold current reductions of around 20% are recorded. Lastly, cross-sectional electron microscope images reveal a simple and versatile method of fabricating buried microlenses from the structure of Wafer B.
A Three-Dimensional Comprehensive Model for Prediction of Macrosegregation in Electroslag Remelting Ingot

ESR is an advanced technology of ingot production which is used in critical applications such as aeronautics, power generation, medicine, and tooling.1) Figure 1 shows the schematic of the ESR process. The passage of an alternating current (AC) from the electrode to the baseplate creates a Joule heating in the highly resistive calcium fluoride-based slag, which is sufficient to melt the electrode. The interaction between the self-induced magnetic field and the AC gives rise to the Lorentz force. Metal droplets sink through the less dense slag to form a liquid metal pool in the watercooled mold. Heat loss to the mold solidifies the metal, forming an ingot with dendritic structure and maintaining a shallow liquid metal pool throughout the process.2) However, macrosegregation occurs during the ESR process due to the relative movement of the solute-rich or poor liquid and solid phases. Moreover, the segregation levels become larger as ingot diameter increases.3) In order to reduce the macrosegregation in the ESR ingots, a deeper understanding of the macroscopic solutal transport should be developed. Given the complicated phenomena involved, and the difficulty and expense of performA Three-Dimensional Comprehensive Model for Prediction of Macrosegregation in Electroslag Remelting Ingot
Properties of ITO on PES film in dependence on the coating conditions and vacuum annealing temperatures

【Transparent conducting indium tin oxide (ITO) films were deposited onto the Polyethersulfone (PES) substrate by using a magnetron sputter type negative metal ion source. In order to investigate the influence of cesium (Cs) partial pressure during deposition and annealing temperature on the optoelectrical properties of ITO/PES film the films were deposited under different Cs partial pressures and post deposition annealed under different annealing temperature from $100^{\circ}C$ to $170^{\circ}C$ for 20 min at $3\;{\times}\;10^{-1}$ Pa. Optoeleetrical properties of ITO films deposited without intentional substrate heating was influenced strongly by the Cs partial pressure and the Cs partial pressure of $1.5\;{\times}\;10^{-3}$ Pa was characterized as an optimal Cs flow condition. By increasing post-deposition vacuum annealing temperature both optical transmission in visible light region and electrical conductivity of ITO films were increased. Atomic force microscopy (AFM) micrographs showed that the surface roughness also varied with post-deposition vacuum annealing temperature. 】
Targeting of Nrf2 induces DNA damage signaling and protects colonic epithelial cells from ionizing radiation

Nuclear factor-erythroid 2–related factor 2 (Nrf2) is a key transcriptional regulator for antioxidant and anti-inflammation enzymes that binds to its endogenous inhibitor protein, Kelch-like ECH (erythroid cell-derived protein with CNC homology)-associated protein 1, in the cytoplasm under normal conditions. Various endogenous or environmental oxidative stresses, such as ionizing radiation (IR), can disrupt the Nrf2–Kelch-like ECH-associated protein 1 complex. This allows Nrf2 to translocate from the cytoplasm into the nucleus to induce transcription of heme oxygenase-1 and other cytoprotective enzymes through binding to antioxidant responsive elements. However, how Nrf2 protects cells from IR-induced damage remains unclear. Here, we report that Nrf2 activation by the synthetic triterpenoids, bardoxolone methyl (BARD) and 2-cyano-3,12-dioxooleana-1,9 (11)-dien-28-oic acid–ethyl amide, protects colonic epithelial cells against IR-induced damage, in part, by enhancing signaling of the DNA damage response. Pretreatment with BARD reduced the frequency of both G1 and S/G2 chromosome aberrations and enhanced the disappearance of repairosomes (C-terminal binding protein interacting protein, Rad51, and p53 binding protein-1 foci) after IR. BARD protected cells from IR toxicity in a Nrf2-dependent manner. The p53 binding protein-1 promoter contains three antioxidant responsive elements in which Nrf2 directly binds following BARD treatment. In addition, 2-cyano-3,12-dioxooleana-1,9 (11)-dien-28-oic acid–ethyl amide provided before exposure to a lethal dose of whole-body irradiation protected WT mice from DNA damage and acute gastrointestinal toxicity, which resulted in improved overall survival. These results demonstrate that Nrf2 activation by synthetic triterpenoids is a promising candidate target to protect the gastrointestinal tract against acute IR in vitro and in vivo.
Tight cocone: a water-tight surface reconstructor

Surface reconstruction from unorganized sample points is an important problem in computer graphics, computer aided design, medical imaging and solid modeling. Recently a few algorithms have been developed that have theoretical guarantee of computing a topologically correct and geometrically close surface under certain condition on sampling density. Unfortunately, this sampling condition is not always met in practice due to noise, non-smoothness or simply due to inadequate sampling. This leads to undesired holes and other artifacts in the output surface. Certain CAD applications such as creating a prototype from a model boundary require a water tight surface, i.e., no hole should be allowed in the surface. In this paper we describe a simple algorithm called Tight Cocone that works on an initial mesh generated by a popular surface reconstruction algorithm and fills up all holes to output a water-tight surface. In doing so, it does not introduce any extra points and produces a triangulated surface interpolating the input sample points. In support of our method we present experimental results with a number of difficult data sets.
A Method for the Identification of Logical Dependencies

Logical dependencies refer to implicit relationships established among software artifacts that make them frequently change together throughout the software development process. Research in global software development has shown that such dependencies influence the quality of software systems and the coordination needs among developers and teams in software development. Properly identifying logical dependencies is thus important. However, several challenges in this topic remain open and must be overcome, such as finding more adequate ways to group related commits and to filter out irrelevant dependencies. Driven by these factors, the goal of the candidate's PhD thesis is to conceive, implement, and evaluate an effective method for logical dependencies identification. Preliminary results have shown that the initial version of our proposed method is feasible even in large scale scenarios and that it is also able to group related commits in atomic-commit-featured version control systems.
Identification of aand 1-Cardiac Myosin Heavy Chain Isoforms as Major Autoantigens in Dilated Cardiomyopathy

Background. Immunization with cardiac myosin induces experimental autoimmune heart disease in genetically predisposed mice. These mice produce heart-specific autoantibodies, some of which are directed against the cardiac myosin isoform. Methods and Results. We have reported the presence of circulating heart-specific autoantibodies in 26% of patients with idiopathic dilated cardiomyopathy (DCM) using indirect immunofluorescence. To identify the autoantigen(s) recognized by heart-specific autoantibodies in human disease, we tested, by Western blotting, sera from 26 DCM patients, 14 of whom were cardiac antibody-positive and 12 antibody-negative, as well as sera from 12 patients with cardiac failure from ischemic or valvular heart disease and from 13 normal subjects who were cardiac antibody-negative. Crude myofibrillar proteins and myosin preparations extracted from human atrial or ventricular specimens were used as antigens. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed. The proteins were electrophoretically transferred to nitrocellulose sheets. The paper strips were incubated in sera from patients or controls at 1:100 dilution; the reaction was revealed with a peroxidase-labeled second antibody against human immunoglobulin. Twelve of the 14 DCM sera (86%) containing heart-specific antibodies reacted with both the a(atrial specific) and ,B(ventricular and slow skeletal) myosin heavy chain isoforms; none of the 13 normal sera (p=0.0001) and one of the 24 heart failure-negative control sera (4%co, p=0.0001) contained antibodies against myosin heavy chain. Conclusions. These findings indicate that aand ,B-cardiac myosin heavy chain isoforms as in the murine model of autoimmune heart disease are major autoantigens in patients with idiopathic DCM. (Circulation 1992;85:1734-1742)
Examination of a simple pulse blanking technique for RFI mitigation

Spectroscopy at L-band can be adversely impacted by radio frequency interference (RFI) due to the presence of numerous sources, especially pulsed RFI from radars operating below 1400 MHz. RFI mitigation is very important to deal with this problem. A simple strategy for reducing pulsed RFI termed “asynchronous pulse blanking” has been implemented in a digital receiver developed at The Ohio State University. This paper presents results from a simulation of the APB algorithm. Several aspects of algorithm use and performance are reported, including means for choosing the algorithm’s parameters and the robustness of the method in a realistic RFI environment. Effects of the blanking process on the final output are also examined.