Miracle-dz/UniFAR-FTUs huggingface dataset:
Polyinosinic acid: polycytidylic acid promotes T helper type 1‐specific immune responses by stimulating macrophage production of interferon‐α and interleukin‐12

The effects exerted on the development in vitro of Dermatophagoides pteronyssinus group I (Der p I)‐specific T cell lines and T cell clones by addition of polyinosinic acid: polycytidylic acid (poly I:C) in lymphocyte bulk culture were examined. Der p I‐specific T cell lines generated in presence of poly I:C exhibited reduced ability to produce interleukin (IL)‐4 and IL‐5 and developed into Der p I‐specific CD4+ T cell clones showing a T helper (Th) type 0 or Th1, instead of Th0/Th2 cytokine profile. This effect was prevented by addition to lymphocyte bulk cultures of a mixture of antibodies specific for interferon (IFN)‐α and IL‐12, whereas the addition of anti‐IFN‐α or anti‐IL‐12 antibody alone was uneffective. Poly I:C also showed the ability to stimulate the production of noticeable amounts of both IFN‐α and IL‐12 by human monocytes. Taken together, these data suggest that poly I:C is a Th1‐inducing agent whose activity is mediated by its ability to stimulate the production of IFN‐α and IL‐12 by monocytes.
Tetrahydrobiopterin augments endothelium‐dependent dilatation in sedentary but not in habitually exercising older adults

Endothelium‐dependent dilatation (EDD) is impaired with ageing in sedentary, but not in regularly exercising adults. We tested the hypotheses that differences in tetrahydrobiopterin (BH4) bioactivity are key mechanisms explaining the impairment in EDD with sedentary ageing, and the maintenance of EDD with ageing in regularly exercising adults. Brachial artery flow‐mediated dilatation (FMD), normalized for local shear stress, was measured after acute oral placebo or BH4 in young sedentary (YS) (n= 10; 22 ± 1 years, mean ±s.e.m.), older sedentary (OS) (n= 9; 62 ± 2), and older habitually aerobically trained (OT) (n= 12; 66 ± 1) healthy men. At baseline, FMD was ∼50% lower in OS versus YS (1.12 ± 0.09 versus 0.57 ± 0.09 (Δmm (dyn cm−2)) × 10−2, P < 0.001; 1 dyn = 10−5 N), but was preserved in OT (0.93 ± 0.08 (Δmm (dyn cm−2)) × 10−2). BH4 administration improved FMD by ∼45% in OS (1.00 ± 0.10 (Δmm (dyn cm−2)) × 10−2, P < 0.01 versus baseline), but did not affect FMD in YS or OT. Endothelium‐independent dilatation neither differed between groups at baseline nor changed with BH4 administration. These results suggest that BH4 bioactivity may be a key mechanism involved in the impairment of conduit artery EDD with sedentary ageing, and the EDD‐preserving effect of habitual exercise.
Somatic and Flagellar Immunofluorescence of Salmonella

Caldwell, W. J. (The Child Research Center of Michigan, Detroit, Mich.), C. S. Stulberg, and W. D. Peterson, Jr. Somatic and flagellar immunofluorescence of Salmonella. J. Bacteriol. 92:1177–1187. 1966. —Labeled globulin fractions of flagellar (H) antisera, prepared against 20 frequently occurring Salmonella serotypes belonging to five major somatic (O) groups, were characterized for O and H immunofluorescence and for O and H agglutinin titers against 32 serotypes. The feasibility of immunofluorescent identification of both somatic and flagellar antigens was enhanced by staining formaldehyde-treated organisms in suspension. Relationships between homologous, partial, and unrelated antigen-antibody systems were then analyzed, and a high degree of correlation was shown between the results obtained by the two serological procedures. Flagellar staining was highly specific, and was bright, faint, or inapparent, depending on the relationship between the antigen-antibody systems involved. Somatic staining was also specific, but somewhat more difficult to interpret, because cells in the same preparation might exhibit a mixture of bright, faint, or no fluorescent intensities. Correlation was shown between the percentage of brightly staining cells found in these preparations and the agglutination titers of the comparable antigen-antibody systems. The phenomenon of a “percentage” reaction was unexplained. Absorption studies further confirmed the specificity of reactions. The techniques developed were applied to surveillance of several mouse colonies for the presence of Salmonella. Broth cultures of fecal specimens were treated with formaldehyde and stained in suspension with “polyvalent” labeled antibody reagents. Agreement was found in 97.6% of the instances between results obtained by immunofluorescence and cultural methods. In addition, preliminary evidence indicated the feasibility of presumptive serotyping of Salmonella isolates by immunofluorescence.
Numerical Study of Sediment Erosion Analysis in Francis Turbine

Effective hydraulic turbine design prevents sediment and cavitation erosion from impacting the performance and reliability of the machine. Using computational fluid dynamics (CFD) techniques, this study investigated the performance characteristics of sediment and cavitation erosion on a hydraulic Francis turbine by ANSYS-CFX software. For the erosion rate calculation, the particle trajectory Tabakoff–Grant erosion model was used. To predict the cavitation characteristics, the study’s source term for interphase mass transfer was the Rayleigh–Plesset cavitation model. The experimental data acquired by this study were used to validate the existing evaluations of the Francis turbine. Hydraulic results revealed that the maximum difference was only 0.958% compared with the CFD data, and 0.547% compared with the experiment (Korea Institute of Machinery and Materials (KIMM)). The turbine blade region was affected by the erosion rate at the trailing edge because of their high velocity. Furthermore, in the cavitation–erosion simulation, it was observed that abrasion propagation began from the pressure side of the leading edge and continued along to the trailing edge of the runner. Additionally, as sediment flow rates grew within the area of the attached cavitation, they increased from the trailing edge at the suction side, and efficiency was reduced. Cavitation–sand erosion results then revealed a higher erosion rate than of those of the sand erosion condition.
Prevention of rare diseases: how revolutionary techniques can help vulnerable individuals—the example of serogroup B meningococcal infection

In countries with established programmes for vaccination of infants, toddlers and adolescents with meningococcal conjugate vaccines, serogroup B invasive meningococcal disease remains the major cause of septicaemia and meningitis in the paediatric and adolescent age groups. Novartis has developed a serogroup B meningococcal vaccine, 4CMenB, to meet this need. We reviewed all 4CMenB studies. The studies found 4CMenB to be highly immunogenic when administered in all schedules, with protective antibody levels (serum bactericidal antibody titres ≥4 or ≥5 with human complement, hSBA) against serogroup B strains expressing vaccine antigens in >95% of vaccinated cohorts. When antibody levels waned, all tested groups demonstrated booster responses. Although possibly an underestimation, the Meningococcal Antigen Typing System (MATS) technique predicts that global coverage of 4CMenB against all serogroup B strains is in the range 66% (Canada) to 91% (USA). The vaccine was found to be generally well tolerated, although local and systemic reactions, notably fever in infants, typical of many vaccines, were increased following concomitant administration of 4CMenB with routine vaccines. When tested, prophylactic paracetamol significantly decreased the frequency and severity of reactions in infants, with no clinically significant impact on immunogenicity of 4CMenB or concomitant routine vaccines. The vaccine is approved for use in the following age groups in the European Union (2 months+), Canada (2 months through 17 years), Australia (2 months+) and Chile (2 months+), following clinical evaluation in 4843 infants and toddlers, and 1712 adolescents and adults, in schedules including a three-dose (2, 3, 4 or 2, 4, 6 months) and a two-dose (6–11 months) infant series with a booster in the second year of life, a two-dose series in toddlers (12–23 months) and children (2–10 years) given 2 months apart (with a booster at least in the EU), and a two-dose series in adolescents (11–17 years) given 1–6 months apart. 4CMenB presents a solution to the unmet medical need of offering protection against serogroup B invasive meningococcal disease in all age groups above 2 months.
Use of Global initiative for asthma (GINA) guidelines in asthma management among paediatric residents in a Sub Saharan African country: a cross-sectional descriptive study

Introduction Clinical practice guidelines are systematically developed statements to assist practitioner and patient decisions about appropriate health care for specific clinical circumstances. Despite abundance of asthma guidelines, prevalence has continued to increase globally. There is need to assess how the contents of asthma guidelines are put to clinical use by doctors in the management of children with asthma. This study aims at evaluating the clinical practice of paediatric residents in applying GINA guidelines. Methods Cross-sectional descriptive study of paediatric residents from 23 university teaching hospitals in Nigeria using structured questionnaire. Data analyses were with Statistical Package for Social Sciences (SPSS) version 19 (Chicago IL). Chi square was used to assess for any significant associations between categorical variables. A p < 0.05 was regarded to be statistically significant. Results Sixty-six paediatric residents aged 27- 40 years were enrolled into the study (37 females and 29 males). One-third had spent more than three years in residency training. Fifty-eight residents (87.9%) were aware of the GINA guidelines while 46 (69.7%) were familiar with its contents. Only 39 (59.1%) residents adhered to the GINA guidelines. Twenty of the 35 junior residents (57.1%) compared to 26 of 31 (83.9%) senior residents were familiar with the GINA guidelines (p=0.031) while 15 of 35 junior residents (42.9%) compared to 24 of 31 senior residents (77.4%) consistently follow the GINA guidelines (p=0.006). Adherence to GINA guidelines was not influenced significantly by years of graduation or training (p>0.05). Conclusion The use of the GINA guidelines was poor among paediatric residents. Application of contents rather than just availability of asthma guidelines may partly account for increasing asthma prevalence globally.
Fabricated Optical Strip Waveguide of Nanophotonics Lithium Niobate

The fabrication of an optical waveguide by designing the device and using a positive photoresist via photo lithography (ultraviolet light) to transfer the pattern of the sample has been studied. A direct coupler method was used to investigate a different wavelength pointer laser beam coupled with a fiber-fiber test coupling coupler employing different wavelength lasers ≈5 mW to measure propagation loss and lattice mismatch. The speed of light and numerical aperture were correlated with refractive index. The result showed a good efficiency of laser transfer to fabricate the optical waveguide.
Real-Time Speech Recognition Systems

SRI is developing the hardware, software, and algorithms necessary to achieve real-time speech-recognition and spoken-language systems. As the first phase of this project, SRI is currently completing the design of a prototype that will be able to recognize from 3,000 to 9,000 words of continuous speech using bigram language models. This prototype is based on four special-purpose integrated circuits. SRI is also continuing to improve the SRI DECIPHER speech recognition system, and enabling it to be incorporated into SRI's architecture for the integration of speech recognition and natural language processing.
Physical activity estimation method by using wireless portable sensor

In this study, an effective noncontact technique for the detection of human physical activity is proposed. The technique is based on detecting the electrostatic induction current generated by the walking motion under non-contact and non-attached conditions. A theoretical model for the electrostatic induction current generated because of a change in the electric potential of the human body is proposed. By comparing the obtained electrostatic induction current with the theoretical model, it becomes obvious that this model effectively explains the behavior of the waveform of the electrostatic induction current. The normal walking motions of daily living are recorded using a portable sensor measurement located in an ordinary house. The obtained results show that detailed information regarding physical activity such as a walking cycle can be estimated using our proposed technique. Additionally, the walking signal is measured when the subject walked with the ankle and knee of the foot fastened to a splint with bandages. This suggests that the proposed technique, which is based on the detection of the walking signal, can be successfully applied to the estimation of the quality of human walking motion.
Insurance Industry Risk During the 2008 Financial Crisis

This paper investigates the impact of the 2007-2009 financial crisis on the volatility of insurance stock returns and on the correlation of the insurance stock returns with the market. In particular, we use a DCC GARCH model that allows for structural breaks in the volatility estimation in order to show the evolution of the insurance stock return volatility and their correlation with the overall market over time. Using daily stock returns on a sample of 208 insurance companies from 1990 until 2011, we find that insurance stock return volatilities increased significantly during the financial crisis but eventually reverted to pre-crisis volatility levels. The crisis also increased the correlation of insurance stocks with the market. The analysis of insurance segments reveals that the correlations with the market exhibit different patterns across segments during the financial crisis.
Gangliosides of liver tumors induced by N-2-fluorenylacetamide. I. Ganglioside alterations in liver tumorigenesis and normal development.

Hyperplastic nodules and hepatocellular carcinomas were induced in livers of rats by a low-protein diet containing 0.05% of the carcinogen N-2-fluorenylacetamide. Ganglioside amounts and composition were determined for histologically different hepatocellular carcinomas and compared with those for control livers, hyperplastic nodules, and liver tissue surrounding hepatomas and nodules as well as those for livers of fetal, newborn, 1-week-old, weanling, and adult Sprague-Dawley rats. Ganglioside sialic acid levels were elevated above those of normal adult liver in all liver tissues following the carcinogen treatment regimen. Livers of fetal and newborn rats contained nearly twice the amount of ganglioside sialic acid on a protein or DNA basis as did livers of adult rats. Analyses of individual nodules and hepatomas revealed two populations of tumors in which the levels of ganglioside sialic acid were 2.3 and 3.8 times normal. Ganglioside sialic acid content was at hepatoma levels in small nodules. Individual gangliosides were evenly distributed between products of the monosialoganglioside and disialoganglioside pathways in normal liver with a ratio of [N-acetylneuraminic acid (sialic acid)] (NAN)-galactose (Gal)-N-acetylgalactosamine (GalNAc)-(NAN)-Gal-glucose (Glc)-ceramide (Cer) (GD1a) to Gal-GalNAc-(NAN)2-Gal-Glc-Cer (GD1b) of about one. In contrast, the monosialogangliosides predominated in liver tissues following administration of the carcinogen. Increased levels of specific monosialogangliosides were present in nodules, in liver of carcinogen-treated animals prior to the appearance of tumors, and in the liver tissues surrounding nodules and hepatomas. In single hepatomas, ganglioside patterns correlated with tumorigenicity. A well-differentiated hepatoma had a normal complement of most gangliosides but was deficient in trisialogangliosides. In a poorly diferentiated but well-circumscribed hepatoma, the relative levels of all higher gangliosides were reduced. The monosialoganglioside Gal-GalNAc-(NAN)-Gal-Glc-Cer (GM1) accounted for 80% of the total ganglioside in a poorly circumscribed and poorly differentiated hepatoma. The ganglioside pattern of fetal livers most closely resembled that of a poorly differentiated hepatoma. During the first week post natum, levels of all higher monosialogangliosides and disialogangliosides declined, but the decline was most pronounced for gangliosides GM1 and GD1a. The ratio of GM1 + GD1a to GD1b + NAN-Gal-GalNAc-(NAN)2-Gal-Glc-Cer or (NAN)3-Gal-Glc-Cer (GT), used as an index of the relative predominance of the monoslaloganglioside and disialoganglioside pathways, fell from 2.7 for fetal liver to 0.4 for adult liver. Pools of precursor gangliosides increased during development, transiently for GalNAc-(NAN)-Gal-Glc-Cer and for more than 3 weeks for NAN-Gal-Glc-Cer. When hyperplastic nodules and hepatocellular carcinomas were compared, a reverse pattern was observed. The ratio of GM1 + GD1a to GD1b + GT rose steadily to values of 2.7 and 11.
Activation of the proto-oncogene p60c-src by point mutations in the SH2 domain

To investigate the importance of a conserved region spanning residues 137 to 241 in the noncatalytic domain of p60c-src (SH2 region), we used oligonucleotide-directed mutagenesis to change residues that are highly conserved in this region. Chicken embryo fibroblasts infected with a p60c-src variant containing arginine instead of tryptophan at residue 148 (W148R) appeared more rounded than cells overexpressing a normal c-src gene, and they formed colonies in soft agar. p60c-src variants containing serine instead of arginine at residue 155 (R155S) or isoleucine instead of glycine at residue 170 (G170I) also appeared transformed and were anchorage independent, but to a lesser extent than W148R. Mutation of residue 201 from histidine to leucine (H201L) had no observable effect. The in vitro kinase activity of cells infected with W148R or G170I was elevated twofold. Expression of p60W148R (or, to a lesser extent, of p60G170I) increased the number of proteins phosphorylated on tyrosine in infected cells. All of the mutants were phosphorylated in vivo on Tyr-527, instead of Tyr-416 as observed for p60v-src. Immunoprecipitated p60W148R and p60G170I were found to be associated with a phosphatidylinositol kinase activity, a factor which appears to be necessary for transformation by tyrosine-specific protein kinases. These results show that a single point mutation in the SH2 region of the cellular src gene can activate its transforming potential. This type of activation is in a new category of alterations at the amino terminus that activate but do not cause a shift in phosphorylation at the carboxy terminus.
Molecular dynamics of EF‐G during translocation

Elongation factor G (EF‐G) plays a crucial role in two stages of mRNA‐(tRNA)2 translocation. First, EF‐G•GTP enters the pre‐translocational ribosome in its intersubunit‐rotated state, with tRNAs in their hybrid (P/E and A/P) positions. Second, a conformational change in EF‐G's Domain IV induced by GTP hydrolysis disengages the mRNA‐anticodon stem‐loops of the tRNAs from the decoding center to advance relative to the small subunit when the ribosome undergoes a backward inter‐subunit rotation. These events take place as EF‐G undergoes a series of large conformational changes as visualized by cryo‐EM and X‐ray studies. The number and variety of these structures leave open many questions on how these different configurations form during the dynamic translocation process. To understand the molecular mechanism of translocation, we examined the molecular motions of EF‐G in solution by means of molecular dynamics simulations. Our results show: (1) rotations of the super‐domain formed by Domains III–V with respect to the super‐domain formed by I–II, and rotations of Domain IV with respect to Domain III; (2) flexible conformations of both 503‐ and 575‐loops; (3) large conformational variability in the bound form caused by the interaction between Domain V and the GTPase‐associated center; (4) after GTP hydrolysis, the Switch I region seems to be instrumental for effecting the conformational change at the end of Domain IV implicated in the disengagement of the codon‐anticodon helix from the decoding center. Proteins 2011; © 2011 Wiley‐Liss, Inc.
Information-based engineering design and the ABET 2000

This paper presents a new approach to teach engineering design to senior undergraduate students and a method used to assess student learning. Recognizing the rapid expansion of information technology, an effort is made to teach a software system used in industry-Pro/ENGINEER-in a design technical elective course. The process of teaching the software is integrated into the process of learning design theory, hands on manufacturing experience, communication and teamwork skills. The participating students are asked to study Engineering Criteria (EC) 2000 and to write a learning essay to evaluate their learning experience using an outcome/assessment matrix. Results obtained from the student evaluation based on Criterion 3 of EC 2000 point out the importance of teaching today's job-related skills. They also strongly indicate the need of teaching the engineering fundamentals that shape students' long-term career prospects.
Organizing medical networked information (OMNI).

The Internet has become a major source of biomedical information over the last 5 years. Several projects have recently been established to help users find respectable information sources quickly. OMNI (Organizing Medical Networked Information) is one such filtering and indexing project. OMNI has focused on the quality of information and the application to Internet resources of standard tools for organizing information such as the National Library of Medicine's Medical Subject Headings and the Dublin Core metadata format. Now two years old, the OMNI project fulfils a valuable role for the UK biomedical community, through its gateway service (http:@omni.ac.uk), its printed resource guides and its training workshop programme. OMNI is also a focus for biomedical metadata activities in the UK. The gateway continues to grow in size and further work on information quality issues and integration is planned.
A pleiotropic element in the medium-chain acyl coenzyme A dehydrogenase gene promoter mediates transcriptional regulation by multiple nuclear receptor transcription factors and defines novel receptor-DNA binding motifs

We previously identified a complex regulatory element in the medium-chain acyl coenzyme A dehydrogenase gene promoter that confers transcriptional regulation by the retinoid receptors RAR and RXR and the orphan nuclear receptor HNF-4. In this study we demonstrate a trans-repressing regulatory function for the orphan receptor COUP-TF at this same nuclear receptor response element (NRRE-1). The transcriptional regulatory properties and receptor binding sequences of each nuclear receptor response element within NRRE-1 are also characterized. NRRE-1 consists of four potential nuclear hormone receptor hexamer binding sites, arranged as [<--1-(n)s-2-->-3-->(n)4<--4], three of which are used in alternative pairwise binding by COUP-TF and HNF-4 homodimers and by RAR-RXR heterodimers, as demonstrated by mobility shift assays and methylation interference analysis. Binding and transactivation studies with mutant NRRE-1 elements confirmed the existence of distinct retinoid, COUP-TF, and HNF-4 response elements that define novel receptor binding motifs: COUP-TF homodimers bound sites 1 and 3 (two hexamer repeat sequences arranged as an everted imperfect repeat separated by 14 bp or ER14), RAR-RXR heterodimers bound sites 1 and 2 (ER8), and HNF-4 homodimers bound sites 2 and 3 (imperfect DR0). Mixing cotransfection experiments demonstrated that the nuclear receptor dimers compete at NRRE-1 to modulate constitutive and ligand-mediated transcriptional activity. These data suggest a mechanism for the transcriptional modulation of genes encoding enzymes involved in cellular metabolism.