Miracle-dz/UniFAR-FTUs huggingface dataset:
Cooperation between the INO80 Complex and Histone Chaperones Determines Adaptation of Stress Gene Transcription in the Yeast Saccharomyces cerevisiae

ABSTRACT In yeast, environmental stresses provoke sudden and dramatic increases in gene expression at stress-inducible loci. Stress gene transcription is accompanied by the transient eviction of histones from the promoter and the transcribed regions of these genes. We found that mutants defective in subunits of the INO80 complex, as well as in several histone chaperone systems, exhibit extended expression windows that can be correlated with a distinct delay in histone redeposition during adaptation. Surprisingly, Ino80 became associated with the ORFs of stress genes in a stress-specific way, suggesting a direct function in the repression during adaptation. This recruitment required elongation by RNA polymerase (Pol) II but none of the histone modifications that are usually associated with active transcription, such as H3 K4/K36 methylation. A mutant lacking the Asf1-associated H3K56 acetyltransferase Rtt109 or Asf1 itself also showed enhanced stress-induced transcript levels. Genetic data, however, suggest that Asf1 and Rtt109 function in parallel with INO80 to restore histone homeostasis, whereas Spt6 seems to have a function that overlaps that of the chromatin remodeler. Thus, chromatin remodeling by INO80 in cooperation with Spt6 determines the shape of the expression profile under acute stress conditions, possibly by an elongation-dependent mechanism.
An enhanced conceptualisation of management teams : adaptive, self-adjusting teams that interact at multiple levels of analysis

This article highlights two themes that emerged from an in-depth multiple-case study of management teams in South African corporations that deserve closer attention in future research. First, it is shown that team composition is not static and changes adaptively in response to changing task demands. Hence, team characteristics such as maturity, or team member characteristics, including skills and competencies, need to be understood and modelled as variable rather than constant. To date, this has not been the case in the management team literature. Second, the findings highlight the prevalence and importance of within-team dynamics with particular reference to subgroup interaction. It is shown that spontaneous, informal sub-group formation is a common and constructive feature of management team functioning. This calls for a reconceptualisation of management teams to acknowledge that they are not indivisible units of analysis. Yet the very essence of the notion of a team, as consistently defined in the management literature, need not be violated or invalidated. The defining attributes of teams, including a common purpose, task interdependence among members and a shared identity may remain intact. What needs to change is our theorising about management teams, to take due cognisance of their dynamic, adaptive, self-regulating functioning in business organisations.
An educational visual prototyping environment for real-time imaging

This paper presents the results of a comparison study using the Visual VIPERS interface, a graphical interface which can be applied as an educational tool for novice computer vision students. The goal of the study was to evaluate the change in usability of the interface after the addition of a monitor tool, which can be used to view intermediate image results at specific stages of an algorithm. A user study was conducted in which participants were asked to find an error in a pre-assembled algorithm. Results indicate that participants using the older version of the interface (with no monitor tool) took, on average, less time to find the error than participants who used the monitor tool. However, interview responses indicated a greater level of understanding of the algorithm from participants who used the monitor tool. Interview responses also demonstrated a clear desire from users of the old interface for the addition of a debugging tool (such as the newly introduced monitor tool). Our belief is that participants using the monitor tool performed a more thorough search of the algorithm, and thus gained a greater understanding of how the algorithm operated, while attempting to determine the source of the error.
Elevated levels and altered fatty acid composition of plasma lysophosphatidlycholine(lysoPC) in ovarian cancer patients

Lysophosphatidylcholine (lysoPC), a product of phosphatidylcholine (PC) hydrolysis via phospholipase A activity, has been proposed to activate cells from a number of lineages. Here, we demonstrate that lysoPC levels are significantly elevated (by 43% overall, relative to normal controls) in the plasma of ovarian cancer patients. This does not appear to be common to all cancers as 5 out of 6 leukemia patients tested had markedly lower (less than one‐half of normal) plasma lysoPC. In the plasma of ovarian cancer patients, the percentages of palmitoyl‐ and stearoyl‐lysoPC species were significantly higher, whereas oleoyl and particularly linoleoyl‐lysoPC were significantly lower than in control subjects. The molar ratios of lysoPC/PC and palmitoyl‐lysoPC/linoleoyl‐lysoPC were also significantly elevated in the plasma of ovarian cancer patients compared with those of control subjects. Furthermore, the calculated value of plasma (lysoPC/PC) × (palmitoyl‐lysoPC/linoleoyl‐lysoPC) was markedly higher in patients compared with controls. Int. J. Cancer, 71:31–34, 1997. © 1997 Wiley‐Liss, Inc.
Self-aligned (top and bottom) double-gate MOSFET with a 25 nm thick silicon channel

In this paper, we report a fabrication method that attains the "ideal" double-gate MOSFET device structure. The top and bottom gates are inherently self-aligned to the source/drain. The source/drain is a fanned-out source/drain structure, which provides a low parasitic resistance. Channel silicon thickness is determined by a planar film deposition process with good uniformity control in principle. N-channel double-gate MOSFET's with a 25 nm thick silicon channel were successfully demonstrated.
Experimental therapy of human prostate cancer by inhibiting MDM2 expression with novel mixed‐backbone antisense oligonucleotides: In vitro and in vivo activities and mechanisms

MDM2 oncogene is overexpressed in many human cancers including prostate cancer and MDM2 levels are associated with poor prognosis. This study was undertaken to investigate the functions of MDM2 oncogene in prostate cancer growth and the value of MDM2 as a drug target for prostate cancer therapy by inhibiting MDM2 expression.
A 0.5V, 400MHz, V/sub 00/-hopping processor with zero-V/sub TH/ FD-SOI technology

A 0.5V, 400MHz, 3.5mW, 16b RISC processor with a 0.25/spl mu/m, dual V/sub T/, fully-depleted SOI technology is presented. Zero V/sub T/ is used in logic for high speed while memories and register files adopt a higher V/sub 00/ and V/sub T/ to suppress leakage. Experimental results show that V/sub 00/-hopping is effective in leakage dominant environments.
Consensus Analysis of Second-Order Multiagent Systems with General Topology and Time Delay

This paper addresses the consensus of second-order multiagent systems with general topology and time delay based on the nearest neighbor rule. By using the Laplace transform technique, it is proved that the second-order multi-agent system in the presence of time-delay can reach consensus if the network topology contains a globally reachable node and time delay is bounded. The bound of time-delay only depends on eigenvalues of the Laplacian matrix of the system. The main contribution of this paper is that the accurate state of the consensus center and the upper bound of the communication delay to make the agents reach consensus are given. Some numerical simulations are given to illustrate the theoretical results.
Intrastate and Interstate Tourism Demand in Australia: An Empirical Analysis

Few studies have investigated intrastate and interstate tourism demand in Australia despite these tourists have different travel characteristics. Using cointegration analysis and error-correction models, this study examines economic determinants of intrastate and interstate tourism, and assesses their relative importance for both types of tourism. Two main findings discovered from this research. First, most of the economic coefficients are not consistent with economic theory. Second, the coefficients for intrastate tourism demand are higher than the coefficients for interstate tourism demand in NSW and WA. This may be useful for developing separate policies and marketing strategies for intrastate and interstate tourism.
Accelerated Production of Antigen-specific T Cells for Preclinical and Clinical Applications Using Gas-permeable Rapid Expansion Cultureware (G-Rex)

The clinical manufacture of antigen-specific cytotoxic T lymphocytes (CTLs) for adoptive immunotherapy is limited by the complexity and time required to produce large numbers with the desired function and specificity. The culture conditions required are rigorous, and in some cases only achieved in 2-cm2 wells in which cell growth is limited by gas exchange, nutrients, and waste accumulation. Bioreactors developed to overcome these issues tend to be complex, expensive, and not always conducive to CTL growth. We observed that antigen-specific CTLs undergo 7 to 10 divisions poststimulation. However, the expected CTL numbers were achieved only in the first week of culture. By recreating the culture conditions present during this first week—low frequency of antigen-specific T cells and high frequency of feeder cells—we were able to increase CTL expansion to expected levels that could be sustained for several weeks without affecting phenotype or function. However, the number of 24-well plates needed was excessive and cultures required frequent media changes, increasing complexity and manufacturing costs. Therefore, we evaluated novel gas-permeable culture devices (G-Rex) with a silicone membrane at the base allowing gas exchange to occur uninhibited by the depth of the medium above. This system effectively supports the expansion of CTL and actually increases output by up to 20-fold while decreasing the required technician time. Importantly, this amplified cell expansion is not because of more cell divisions but because of reduced cell death. This bioprocess optimization increased T-cell output while decreasing the complexity and cost of CTL manufacture, making cell therapy more accessible.
New Active Stacked NPC Multilevel Converter: Operation and Features

In this paper, the operation and the features of a new three-level converter are presented. The proposed topology was named three-level active-stacked neutral point clamped (3L-ASNPC). It is a derivative of the 3L-SNPC structure, having two additional active switches connected antiparallel with the clamp diodes. The main advantage of 3L-ASNPC converter is the reduction of the average switching frequency for all power devices. In the same time, the apparent switching frequency of the output voltage is doubled. Experimental and simulation results are shown in order to validate the proposed structure and the analysis of the switching states.
Mixed primary culture and clonal analysis provide evidence that NG2 proteoglycan‐expressing cells after spinal cord injury are glial progenitors

NG2+ cells in the adult rat spinal cord proliferate after spinal cord injury (SCI) and are postulated to differentiate into mature glia to replace some of those lost to injury. To further study these putative endogenous precursors, tissue at 3 days after SCI or from uninjured adults was dissociated, myelin partially removed and replicate cultures grown in serum‐containing or serum‐free medium with or without growth factors for up to 7 days in vitro (DIV). Cell yield after SCI was 5–6 times higher than from the normal adult. Most cells were OX42+ microglia/macrophages but there were also more than twice the normal number of NG2+ cells. Most of these coexpressed A2B5 or nestin, as would be expected for glial progenitors. Few cells initially expressed mature astrocyte (GFAP) or oligodendrocyte (CC1) markers, but more did at 7 DIV, suggesting differentiation of glial precursors in vitro. To test the hypothesis that NG2+ cells after SCI express progenitor‐like properties, we prepared free‐floating sphere and single cell cultures from purified suspension of NG2+ cells from injured spinal cord. We found that sphere cultures could be passaged in free‐floating subcultures, and upon attachment the spheres clonally derived from an acutely purified single cell differentiated into oligodendrocytes and rarely astrocytes. Taken together, these data support the hypothesis that SCI stimulates proliferation of NG2+ cells that are glial progenitor cells. Better understanding the intrinsic properties of the NG2+ cells stimulated by SCI may permit future therapeutic manipulations to improve recovery after SCI. © 2007 Wiley Periodicals, Inc. Develop Neurobiol, 2007.
A Sad State of Repair - Liability for Maintaining Leased Business Premises

Covenants for the care and maintenance of business premises receive little attention when leases are negotiated, yet these covenants often cause arguments later because of onerous or uncertain liabilities. When repair or outgoings covenants are not explicit, current laws provide little protection for either the landlord or the tenant. Generally, landlords' solicitors draft covenants to the advantage of landlords but legal disputes are endemic. This article suggests ways in which the lease covenants that govern the repair and management of business premises could be drafted to achieve more harmony.
A Parallel Algorithm to Find Overlapping Community Structure in Directed and Weighted Complex Networks

Based on the BSP (Bulk Synchronous Parallel) computing framework, this paper proposes a parallel algorithm to find overlapping community structure in directed and weighted complex networks. The algorithm is composed of four parts, job distribution, finding natural communities, passing information in cluster and merging the similar natural communities. The main idea of the algorithm is finding the local maxima of a modularity function by local, iterative search to ensure that each vertex in network at least belongs to one natural community. By iteratively merging the similar natural communities we get a series of schemes and choose the scheme which results in the maximum modularity as the best scheme. By using Hama which is a pure BSP computing framework, we conduct experiments in several real networks. The results show the algorithm can give an original scheme very fast and give the best scheme with high accuracy.
Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins.

In the budding yeast Saccharomyces cerevisiae, the G1 cyclins Cln1, Cln2 and Cln3 regulate entry into the cell cycle (Start) by activating the Cdc28 protein kinase. We find that Cln3 is a much rarer protein than Cln1 or Cln2 and has a much weaker associated histone H1 kinase activity. Unlike Cln1 and Cln2, Cln3 is not significantly cell cycle regulated, nor is it down‐regulated by mating pheromone‐induced G1 arrest. An artificial burst of CLN3 expression early in G1 phase accelerates Start and rapidly induces at least five other cyclin genes (CLN1, CLN2, HCS26, ORFD and CLB5) and the cell cycle‐specific transcription factor SWI4. In similar experiments, CLN1 is less efficient than CLN3 at activating Start. Strikingly, expression of HCS26, ORFD and CLB5 is dependent on CLN3 in a cln1 cln2 strain, possibly explaining why CLN3 is essential in the absence of CLN1 and CLN2. To explain the potent ability of Cln3 to activate Start, despite its apparently weak biochemical activity, we propose that Cln3 may be an upstream activator of the G1 cyclins which directly catalyze Start. Given the large number of known cyclins, such cyclin cascades may be a common theme in cell cycle control.
Feature Extraction Techniques in Speech Processing: A Survey

Speech processing includes the various techniques such as speech coding, speech synthesis, speech recognition and speaker recognition. In the area of digital signal processing, speech processing has versatile applications so it is still an intensive field of research. Speech processing mostly performs two fundamental operations such as Feature Extraction and Classification. The main criterion for the good speech processing system is the selection of feature extraction technique which plays an important role in the system accuracy. This paper intends to focus on the survey of various feature extraction techniques in speech processing such as Fast Fourier Transforms, Linear Predictive Coding, Mel Frequency Cepstral Coefficients, Discrete Wavelet Transforms, Wavelet Packet Transforms, Hybrid Algorithm DWPD and their applications in speech processing.
Educational Commitment: The Immigrant Advantage

Popular press accounts of the educational success of immigrants in the United States often attribute these positive outcomes to the behaviors of immigrant parents, who are described as more authoritarian and intensively focused on their children’s schoolwork than native-born parents in the United States. Although children who were born outside the United States do have more pro-school attitudes than native-born youth, less research has tested whether parental behaviors actually explain this difference. Using the Educational Longitudinal Study (ELS:2002), we test generational differences in the “educational commitment” of high school sophomores, comparing their academic interest and purpose. We find that, across both of these educational attitudes and regardless of national origin, first-generation youth are the most educationally committed, followed by second-generation youth and then those in the third or higher generation. These generational gaps are not explained by differences in parental behaviors, earlier measures of academic ability and expectations, perceptions of discrimination, or peer influences. However, interesting ethnic differences emerge and suggest that the familial and school context may be more important for understanding the educational commitment of Mexican-origin students than East and South Asian students. We find that, among the firstgeneration, living in the United States for a longer time is associated with less educational commitment as well. Finally, we explore the importance of these findings for future explorations of the “immigrant advantage” in educational attitudes and outcomes, comparing explanations that focus on the amount of contact with institutions in the U.S. to explanations that focus on the characteristics of the parents.
Cardiac KATP channel modulation by 16Hz magnetic fields — A theoretical study

Heart exposure to 16Hz magnetic fields (MFs) was shown to be cardio-protective for diseased hearts; still, the mechanism of this effect is unknown. We hypothesize that a possible one mechanism is an increased trans-membrane KATP channel open probability due to modulation of the degree of dissociation between K+ ions, having a resonance frequency of 16Hz, and the channel selectivity filter. The Fan-Makielski Markovian KATP channel model was adopted, and the MF bio-effect was manifested by modulating the open probability of the channel using the predictive MF bio-effect parameter based on Binhi's quantum mechanics model. The model was integrated in a ventricular single cell model and the MF effect on the calcium transients [Ca2+] was assessed. Periodic pacing (Cycle Length CL=1sec) was applied and a 16Hz or 32Hz MF was turned on at t=0 for 10min. MF exposure gradually decreased [Ca2+] due to KATP channel opening, more strongly at 16Hz. Additionally, a small negative diastolic shift was observed. These numerical results demonstrated similarity to published experimental data using similar 16Hz MF exposure. We conclude that 16Hz MF exposure increases the KATP channel open probability, lowering the cellular calcium load. Our model could be integrated in a tissue model to predict optimal MF parameters for future cardiac therapy devices.
On the use of variational mode decomposition for removal of baseline wander in ECG signals

Automatic detection and removal of baseline wanders is most important for accurate measurement of clinical features of local waves including, P-wave, QRS complex, T-wave and U-wave of the ECG signals. In this paper, we investigate on the use of variational mode decomposition (VMD) technique for removal of baseline wander in ECG signals. The proposed method consists of three stages: VMD based ECG signal decomposition, baseline wander extraction using frequency criterion, and base-line wander subtraction. The method is tested and evaluated using a wide variety of ECG signals including different kinds of PQRST morphologies corrupted with different patterns of baseline wanders. Performance evaluation results show that the VMD based method can adequately remove the baseline wanders without significantly distorting local components (P-wave, QRS complex, ST-segment, T-wave) of the ECG signal. The noise reduction capability of the VMD-based method is compared with the existing methods using the wavelet transform and Fourier transform. The subjective and objective quality assessment results show that the VMD based method outperforms the existing methods in terms achieving better objective quality metric values for various noisy ECG signals.
Research on virtual exhibition interaction and user experience technology

User experience is the subjective feeling of the product. In virtual environment, the user experience is the user¿s interaction with the virtual environment; generated behaviors by the expectations, perceptions, feelings and others; and the memory, attitudes, feelings after use. This paper discussed the user experience model based on the computer multimedia environment and implementation of the virtual exhibition prototype system.
Gain-Improved Broadband Circularly Polarized Antenna Array With Parasitic Patches

A broadband circularly polarized (CP) antenna array with improved gain is proposed. The antenna consists of a CP loop that provides sequential phase, a 2 × 2 patch array, and four parasitic rectangular elements. The square patches are capacitively coupled with four strips, which are connected to the loop feeding network. Moreover, four parasitic rectangular patches are introduced to enhance the gain in the lower band. The measured 10-dB impedance bandwidth is 15.9% (5.01–5.87 GHz), and the 3-dB axial-ratio bandwidth of 640 MHz (5.08–5.72 GHz) is also obtained. Besides, the proposed antenna array has a flat gain over the operating frequency and the maximum gain of 12.5 dBi is achieved at 5.3 GHz.
GaN-Based White LEDs With CIS/ZnS Quantum Dots Synthesized Using Polyetheramine as Solvent

We propose a fast and low-temperature method to synthesize CuInS2/ZnS quantum dots (CIS/ZnS QDs) using polyetheramine (D400) as solvent. It was found that the CIS/ZnS QDs synthesized at 140 °C exhibited a broad photoluminescence (PL) emission bandwidth (i.e., 135 nm) and a small PL blueshifted (i.e., 20 nm) as we coated the ZnS shells onto the CIS cores. Compared with the conventional GaN-based white light-emitting diodes with phosphor only, it was found that we could improve color rendering index and correlated color temperature by adding the CIS/ZnS QDs.