Miracle-dz/UniFAR-FTUs huggingface dataset:
Multiplex Detection of RNA Expression in Drosophila Embryos

We present a fluorescence-based, multiplex in situ hybridization method that permits the simultaneous detection of five differently labeled antisense RNA probes and up to seven different transcripts in a single Drosophila embryo. We also show that it should be possible to increase the number of detected transcripts substantially with nascent transcript multiplex fluorescent in situ hybridization. These multiplex methods fill a current technological gap between high-resolution in situ hybridization with one or two fluorescently labeled probes and low-resolution but genome-wide microarray RNA profiling and should be of great utility in establishing gene networks.
Gait retraining as part of the treatment programme for soldiers with exercise-related leg pain: preliminary clinical experiences and retention

Background: Gait retraining as part of a treatment programme for exercise-related leg pain (ERLP) was introduced in the sports medicine department of the Royal Netherlands Army in 2013.Objectives: To describe clinical experiences and retention of gait retraining in a military setting.Methods: Sixty-one cases from the year 2015 were available for analysis of gait and gait retraining. In 2016, 32 of these patients were available for a follow-up survey, 28 of them also for the follow-up measurement of running biomechanics in running shoes.Results: Soldiers received an outpatient treatment programme that lasted on average 129 days (SD 76). On average they received 2.4 gait retraining sessions, leading to significant and lasting changes in running biomechanics; in particular, reduction in maximal force (N) and maximal pressure (N/cm2) on the heels at 317 days follow-up (average, SD 108). Most soldiers were satisfied with gait retraining. At follow-up, 27 soldiers (84%) contributed some, the majority or all reduction of symptoms to it. Seventy percent reported that they had mastered the new running technique within two months. The Single Assessment Numeric Evaluation score increased from 55% to 78% for males and from 44% to 75% for females.Discussion: This is the first study to report on gait retraining for Medial Tibial Stress Syndrome. In future, prospective studies in the military running in shoes and running in boots respectively should be investigated.Conclusion: Soldiers with exercise-related leg pain (ERLP), among them patients with Medial Tibial Stress Syndrome, respond well to a treatment programme that included gait retraining. Ten months post-gait retraining, their running biomechanics still showed these positive changes from their time of intake.
Influences of partners' views of asthma self-management and family environment on asthmatic adults' asthma quality of life.

Influences of partners' views of the adult asthmatic's self-management and family environment were explored from the perspective of a collaborative care system. Adults (N = 172, 86 couples) completed questionnaires: perceived control of asthma, perceived ability to care for self, asthma quality of life (AQOL), and an asthma opinion survey. Significant relationships between perceptions of adults with asthma (AWA) and partners were found (r ranged from.32 to.74). Multiple regression analysis of partners' perceptions on AWA AQOL yielded a 3-factor solution (R(2) = 39%). Family cohesion had a significant effect on AWA AQOL (F [1, 168] = 4.43, p <or=.04). Clinical implications are discussed.
Jointly Optimizing Activation Coefficients of Convolutive NMF Using DNN for Speech Separation

Convolutive non-negative matrix factorization (CNMF) and deep neural networks (DNN) are two efficient methods for monaural speech separation. Conventional DNN focuses on building the non-linear relationship between mixture and target speech. However, it ignores the prominent structure of the target speech. Conventional CNMF model concentrates on capturing prominent harmonic structures and temporal continuities of speech but it ignores the non-linear relationship between the mixture and target. Taking these two aspects into consideration at the same time may result in better performance. In this paper, we propose a joint optimization of DNN models with an extra CNMF layer for speech separation task. We also utilize an extra masking layer on the proposed model to constrain the speech reconstruction. Moreover, a discriminative training criterion is proposed to further enhance the performance of the separation. Experimental results show that the proposed model has significant improvement in PESQ, SAR, SIR and SDR compared with conventional methods.
What the user does not want?: query reformulation through term inclusion-exclusion

In information retrieval, keyword-based queries often fail to capture actual information need, especially when the need is very specific and particular. Using natural language, however, a user can clearly tell what she wants (positive part) and what she does not (negative parts). We propose techniques for automatic removal of negative parts and query augmentation with judicious term inclusion-exclusion from negative parts. Experiments conducted on standard datasets like TREC, ROBUST, WT10G demonstrate that the proposed techniques yield substantial performance gain, often being statistically significant.
A new time-mode on-chip oscillator-based low power temperature sensor

This paper presents an energy-efficient and high linearity temperature sensor based on simple on-chip oscillator's architectures. A self-calibrated architecture is proposed to compensate the nonlinear effects due to PVT variations. In this manner, this on-chip oscillator-based temperature sensor has superior performance over the conventional inverter-chain based types. In order to generalize the application, no highly linear temperature coefficient resistors are being utilized. The entire circuit is simple and easy to be scaled down. According to the verification in 65nm CMOS process, with one-point calibration, this temperature sensor can achieve an inaccuracy within 1°C in the temperature range from -55°C to 125°C, with a power consumption of only 0. 6μA under 1.2V supply voltages.
Computer control of carbon–nitrogen ratio in Spirulina platensis

An on‐line computer was used to control the ratio of carbon to nitrogen in algal biomass. An indirect method of growth and biomass estimation was utilized. This was based on balancing the amount of CO2 carbon in and out of the algal bioreactor. It was shown that growth conditions govern the morphology and composition of Spirulina platensis. Cells grown under light limitation were narrower, had high levels of phycocyanin pigments, and were packed full of small lipid granules. Whereas cells grown under nitrogen limitation lost their characteristic blue‐green color, had reduced levels of phycocyanin, were fatter, and were packed full of larger lipid granules.
Endocrinal and autoimmune linkage: Evidences from a controlled study of subjects with polycystic ovarian syndrome

BACKGROUND: Polycystic ovary syndrome (PCOS) is a metabolic syndrome, characterized by anovulation, hyperandrogenism, and polycystic ovary. With serological markers of autoimmunity found elevated in PCOS, there is a possible link between autoimmunity and PCOS. AIM: The study aimed to investigate the possible correlation between autoimmune markers of autoimmune thyroiditis (AIT) and PCOS. SETTING AND DESIGN: This case control study was conducted at the Department of Pathology of a tertiary care academic center during a 1-year period. MATERIALS AND METHODS: Fifty-five subjects with clinical PCOS and 51 age matched control non-PCOS subjects were recruited and subjected to clinical, biochemical, and endocrinal evaluation for AIT. All subjects underwent blood glucose and serum sampling for luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone, dehydroepi androsterone, thyroxine, thyroid stimulating hormone, anti-thyroid peroxidase, anti-thyroglobulin (Tg), and insulin. STATISTICAL ANALYSIS: Statistical analysis was performed using SPSS version 12 for Windows. The quantitative variables are described as mean ± standard deviation. To compare quantitative variables between two groups, unpaired t-test was used. The Chi-square/Fischer's exact test was used to compare qualitative variables. ANOVA was used to compare the PCOS and non-PCOS groups. P < 0.05 was considered significant. RESULTS: Significantly higher prevalence of AIT (anti-Tg antibodies) was noted in subjects with PCOS as compared to non-PCOS control subjects (P < 0.05). The PCOS subjects had higher insulin resistance index and also twice the level of LH: FSH ratio as compared to controls. CONCLUSION: Higher prevalence of AIT in PCOS subjects suggest possible role of autoimmune phenomenon in the etiopathogenesis of PCOS. More data from longitudinal follow-up studies is required to clearly establish this possible link.
Model predictive control applied to a single phase seven-level active rectifier

This paper presents an improved single phase seven-level active rectifier architecture controlled by finite control set model predictive control (FCS-MPC). The FCS-MPC is designed to enable power conversion with a unity power factor and generate seven level voltage waveform at the input. The proposed active rectifier architecture reduces harmonic contents of the rectifier input current by producing different voltage levels at the rectifier input. Owing to the architecture and multilevel operation, it reduces the EMI filter size, input current harmonic, the voltage rating on devices and switching losses that are lower than those of conventional three-level rectifier topologies. The proposed converter can also be operated as a multilevel inverter. Extensive simulation results are presented to verify the proposed converter when the load changes, the reference active and reactive power changes.
On the species status of the root-knot nematode Meloidogyne mayaguensis Rammah & Hirschmann, 1988

Abstract Holo- and paratypes of the root-knot nematodes Meloidogyne mayaguensis Rammah & Hirschmann, 1988 and Meloidogyne enterolobii Yang & Eisenback, 1983 were morphometrically and morphologically compared. All observed female, male and second-stage juvenile morphometrical and morphological characters are identical for the two studied species. Additionally, contradictions between the original species descriptions were unravelled. The present study of holo- and paratypes confirms the taxonomical status of Meloidogyne mayaguensis as a junior synonym for Meloidogyne enterolobii.
Femmes et hommes face à l'ordinateur. Histoires du développement d'une relation positive

Lorsqu'on parle de l'usage de l'ordinateur par des personnes adultes, il est classique que soient evoquees un certain nombre de disparites femmes/hommes qui mettent notamment l'accent sur une relation plutot negative des femmes a l'ordinateur. Les etudes realisees visant a proposer des moyens pour diminuer ces disparites se basent le plus souvent sur des informations recueillies aupres de professionnel-le-s ou expert-e-s ; ce qui laisse dans l'ombre une grande partie des utilisateurs/trices ordinaires. On dispose donc de peu d'informations sur ces personnes qui, sans etre expertes ou professionnelles, apprecient l'ordinateurs et l'utilisent regulierement sans hesiter. C'est a ces personnes que la presente these a choisi de s'interesser. Son objectif principal est de collecter des informations concernant ce qui fonde la relation positive a l'ordinateur, que developpent des femmes et hommes qui en font un usage regulier mais non professionnel. Dans ce but, vingt-cinq entretiens narratifs ont ete realises. Prenant appui sur une comparaison intersexe, les analyses realisees explorent l'existence d'eventuelles disparites femmes/hommes et le role potentiel que joue le genre, apprehende en tant que systeme hierarchisant de normes de sexe . Les resultats montrent que ces relations positives s'appuient, pour les femmes comme pour les hommes, sur les memes elements et que l'influence du genre depend des enjeux de pouvoirs et de la reconnaissance des situations considerees comme defiant l'imaginaire qui voit les hommes plus proches de la technologie que les femmes.
Development and distribution of immunoglobulin-containing cells in the chicken. An immunofluorescent analysis using purified antibodies to mu, gamma and light chains.

The development and distribution of immunoglobulin-containing cells were studied in chickens using purified antibodies to µ, γ and light chains labeled with fluorochromes. Cells containing µ and light chains were found in lymphoid follicles of the bursa of Fabricius of 14-day embryos. IgG-containing cells were initially observed in bursal follicles at 21 days of incubation. Embryonic infusions of plasma from newly hatched and adult chickens had no effect on the appearance of immunoglobulin-containing cells within the bursa. The developmental sequence of immunoglobulin-containing cells within the bursa was later recapitulated in the spleen, tonsilla caecalis and thymus. Rapid expansion of IgM- and IgG-containing cells began in the spleen on the 3rd and 8th days, respectively, after hatching. The ratio of IgG- to IgM-containing cells was greatest in the intestinal wall of immunologically mature birds. Development of immunoglobulin-containing cells in extra-bursal sites was retarded in chickens hatched under germ-free conditions. In contrast, neither exclusion of exogenous antigens nor antigenic stimulation in ovo affected the time of appearance or proliferation of immunoglobulin-containing cells within the bursa. The medullary regions of individual bursal follicles frequently stained for both IgM and IgG. This pattern was not observed in lymphoid follicles of other tissues. Analysis of single cell suspensions revealed a high proportion of single bursal cells containing heavy chains of both IgM and IgG, wheras spleen cells rarely contained both classes of immunoglobulins. The results suggest that a developmental switch from IgM to IgG synthesis may occur within the bursa.
Probing the relevance of NHC ligand conformations in the Ru-catalysed ring-closing metathesis reaction.

Probing the Relevance of NHC Ligand Conformations in the Ru-Catalysed Ring-Closing Metathesis Reaction Cat power : Judicious backbone substitution of N-heterocyclic carbenes (NHCs) leads to stable Ru metathesis catalysts with frozen NHC conformations. This finding not only permits the isolation of complexes that are among the most active catalysts in the ringclosing metathesis of hindered olefins (see graphic; Ts= p-toluenesulfonyl), but also provides fundamental mechanistic insights on the role of N-aryl substituent conformations on catalyst activity.
Sample/Hold, V2I and output latching techniques for an asynchronous low area ADC

The architecture of an asynchronous CMOS Analogue to Digital Converter (ADC) with a binary tree structure exhibiting ultra low die area and power consumption will be presented in this paper. It is based on integer division that is implemented by current mode circuits that operate without a clock signal. Special emphasis is given on the description of the Sample/Hold and Voltage to Current conversion method at the input of such an asynchronous ADC as well as in the selection of an appropriate clock for the latching of correct codes at the ADC output and the relevant trade offs. The designed 8-bit ADC occupies 0.06mm2 area and dissipates 32mW power while its active throughput is 150MS/s.
The Mediterranean-style diet for the prevention of cardiovascular diseases

Abstract Objectives To discuss present knowledge about Mediterranean diet and cardiovascular diseases. Design Review of existing literature. Setting and Results Epidemiological studies as well as randomised dietary trials suggest that Mediterranean diet may be important in relation to the pathogenesis (and prevention) of CHD. For instance, a striking protective effect of an ALA-rich Mediterranean diet was reported in the Lyon Diet Heart Study with a 50 to 70% reduction of the risk of recurrence after 4 years of follow-up in CHD patients. According to our current knowledge, dietary ALA should represent about 0.6 to 1% of total daily energy or about 2 g per day in patients following a Mediterranean diet, whereas the average intake in linoleic acid should not exceed 7 g per day. Supplementation with very-long-chain omega-3 fatty acids (about 1 g per day) in patients following a Mediterranean type of diet was shown to decrease the risk of cardiac death by 30% and of sudden cardiac death by 45% in the GISSI trial. Conclusions In the context of a diet rich in oleic acid, poor in saturated fats and low in omega-6 fatty acids (a dietary pattern characterising the traditional Mediterranean diet), even small doses of omega-3 fatty acids (about 1 g EPA + DHA the form of fish oil capsules or 2 g α-linolenic acid in canola oil and margarine) might be very protective. These data underline the importance of the accompanying diet in any dietary strategy using fatty acid complements.
Diagnosis and management of schistosomiasis

The authors’ studies on schistosomiasis have received financial support from various sources including: the UNICEF/UNDP/World Bank/WHO Special Program for Research and Training in Tropical Diseases; the National Health and Medical Research Council of Australia; the Wellcome Trust (UK); the Sandler Foundation (USA); the Dana Foundation (USA); and the National Institute of Allergy and Infectious Diseases.
Suicidal ideation and its correlates in prisoners: a comparative study in China.

BACKGROUND Suicide is one of the leading causes of prisoners' death, and, as a group, inmates have higher suicide rates than their community counterparts. However, the prevalence of suicidal ideation among Chinese inmates and their risks of suicide remain unknown. AIMS This study investigates the prevalence of suicidal ideation among Chinese incarcerated populations and its risk and protective factors. METHODS We randomly selected three types of prisoners (N = 690) and a sample of college students (n = 248) from four correction facilities and a police academy in China. A self-report questionnaire with standardized scales translated from the West was administered to the samples. RESULTS The prevalence of suicidal ideation among Chinese inmates is similar to that found among inmates in the West. The correlates of suicidal ideation for the Chinese inmates are also in line with what has been found in general populations. CONCLUSIONS It is suggested that Chinese inmates have the same risk factors and protective factors for suicidal ideation as the general population in China. Suicide prevention in Chinese prisons can learn from and inform suicide prevention work for the general population.
Vagus Nerve Stimulation Delivered During Motor Rehabilitation Improves Recovery in a Rat Model of Stroke

Neural plasticity is widely believed to support functional recovery following brain damage. Vagus nerve stimulation paired with different forelimb movements causes long-lasting map plasticity in rat primary motor cortex that is specific to the paired movement. We tested the hypothesis that repeatedly pairing vagus nerve stimulation with upper forelimb movements would improve recovery of motor function in a rat model of stroke. Rats were separated into 3 groups: vagus nerve stimulation during rehabilitation (rehab), vagus nerve stimulation after rehab, and rehab alone. Animals underwent 4 training stages: shaping (motor skill learning), prelesion training, postlesion training, and therapeutic training. Rats were given a unilateral ischemic lesion within motor cortex and implanted with a left vagus nerve cuff. Animals were allowed 1 week of recovery before postlesion baseline training. During the therapeutic training stage, rats received vagus nerve stimulation paired with each successful trial. All 17 trained rats demonstrated significant contralateral forelimb impairment when performing a bradykinesia assessment task. Forelimb function was recovered completely to prelesion levels when vagus nerve stimulation was delivered during rehab training. Alternatively, intensive rehab training alone (without stimulation) failed to restore function to prelesion levels. Delivering the same amount of stimulation after rehab training did not yield improvements compared with rehab alone. These results demonstrate that vagus nerve stimulation repeatedly paired with successful forelimb movements can improve recovery after motor cortex ischemia and may be a viable option for stroke rehabilitation.
Predicting CHD risk in France: a pooled analysis of the D.E.S.I.R., Three City, PRIME, and SU.VI.MAX studies

Background: We aimed to develop and validate a simple coronary heart disease (CHD) risk algorithm applicable to asymptomatic men and women in France, and to compare its accuracy with that of the last published version of the Framingham risk function for cardiovascular disease. Design: A pooled analysis of four French prospective general-population studies. Methods: The baseline and follow-up data from D.E. S.I. R. , PRIME, Three City, and SU.VI. MAX studies were used. The 10-year CHD risk was estimated by the Cox proportional hazards model with candidate variables including age, gender, body mass index, waist circumference, family history of coronary heart disease, smoking status, diabetes status, systolic blood pressure, and total and high-density lipoprotein (HDL) cholesterol. Results: The study population included 22,256 subjects (61.4% men) aged (SD) 56.0 years (8.3) without a personal history of CHD at baseline. After a mean follow-up of 8.0 years (2.3), 788 first CHD events occurred, 726 in men and 62 in women. The final model included age, gender, age × gender interaction, current smoking status, diabetes status, systolic blood pressure, total and HDL cholesterol. Using this model, the number of predicted coronary events fitted that given by the 10-year Kaplan–Meier survival estimates within each decile of estimated risk (calibration). This model had fair discrimination: Harrell C-index, 0.7831 (95% CI: 0.7704–0.7957). For comparison, the recalibrated Framingham risk function had equivalent performances compared to the French risk equation. Conclusion: Our 10-year French CHD risk equation based on traditional risk factors performed at least as well as the recalibrated Framingham cardiovascular disease risk function.