Miracle-dz/UniFAR-FTUs huggingface dataset:
Reply: Comparison of 64-Slice CT with Gated SPECT for Evaluation of Left Ventricular Function

UNLABELLED Precise and reliable assessment of left ventricular (LV) function and dimensions is prognostically important in cardiac patients. As the integration of SPECT and multislice CT into hybrid scanners will promote the combined use of both techniques in the same patient, a comparison of the 2 methods is pertinent. We aimed at comparing LV dimensions, muscle mass, and function obtained by electrocardiographically gated 64-slice CT versus gated-SPECT. METHODS Sixty patients (mean age, 64 +/- 8 y) referred for evaluation of coronary artery disease underwent 99mTc-tetrofosmin gated SPECT and 64-slice CT within 4 +/- 2 d. LV ejection fraction (LVEF), end-systolic volume (ESV), and end-diastolic volume (EDV) from CT were compared with SPECT. Additionally, LV muscle mass and quantitative regional wall motion were assessed in 20 patients with both methods. RESULTS CT was in good agreement with SPECT for quantification of LVEF (r = 0.825), EDV (r = 0.898), and ESV (r = 0.956; all P < 0.0001). LVEF was 59% +/- 13% measured by SPECT and slightly higher but not significantly different by CT (60% +/- 12%; mean difference compared with SPECT, 1.1% +/- 1.7%; P = not significant). A systematic overestimation using CT for EDV (147 +/- 60 mL vs. 113 +/- 52 mL; mean difference, 33.5 +/- 23.1 mL) and ESV (63 +/- 55 mL vs. 53 +/- 49 mL; mean difference, 9.3 +/- 15.9 mL; P < 0.0001) was found compared with SPECT. A good correlation for muscle mass was found between the 2 methods (r = 0.868; P < 0.005). However, muscle mass calculated by SPECT was significantly lower compared with CT (127 +/- 24 g vs. 148 +/- 37 g; mean difference, 23.0 +/- 12.2 g; P < 0.001). The correlation for regional wall motion between the 2 methods was moderate (r = 0.648; P < 0.0001). CONCLUSION LVEF and LV functional parameters as determined by 64-slice CT agree over a wide range of clinically relevant values with gated SPECT. However, interchangeable use of the 2 techniques should be avoided for LV volumes, muscle mass, and regional wall motion because of variances inherent to the different techniques.
Sectorized Antenna-based DoA Estimation and Localization: Advanced Algorithms and Measurements

Sectorized antennas are a promising class of antennas for enabling direction-of-arrival (DoA) estimation and successive transmitter localization. In contrast to antenna arrays, sectorized antennas do not require multiple transceiver branches and can be implemented using a single RF front-end only, thus reducing the overall size and cost of the devices. However, for good localization performance the underlying DoA estimator is of uttermost importance. In this paper, we therefore propose a novel high performance DoA estimator for sectorized antennas that does not require cooperation between the transmitter and the localizing network. The proposed DoA estimator is broadly applicable with different sectorized antenna types and signal waveforms, and has low computational complexity. Using computer simulations, we show that our algorithm approaches the respective Cramer-Rao lower bound for DoA estimation variance if the signal-to-noise ratio (SNR) is moderate to large and also outperforms the existing estimators. Moreover, we also derive analytical error models for the underlying DoA estimation principle considering both free space as well as multipath propagation scenarios. Furthermore, we also address the fusion of the individual DoA estimates into a location estimate using the Stansfield algorithm and study the corresponding localization performance in detail. Finally, we show how to implement the localization in practical systems and demonstrate the achievable performance using indoor RF measurements obtained with practical sectorized antenna units.
Semantic interpretation and the resolution of ambiguity: Dedication

Preface 1. Introduction 2. Semantic interpretation 3. The Absity semantic interpreter 4. Lexical disambiguation 5. Polaroid words 6. Structural disambiguation 7. The semantic enquiry desk 8. Conclusion 9. Speculations, partially baked ideas, and exercises for the reader References Index of names Index of subjects.
Impact of beamforming strategies and regularisation on ultrasound displacement estimation using RF-based image registration

In the context of ultrasound (US) deformation imaging it has been shown that lateral motion estimation can be improved by simultaneously combining transverse oscillation (TO) beamforming with dedicated motion estimators and regularisation techniques. This paper provides insights into the relative contributions of beamforming strategies (focused imaging [FOC], plane wave TO beamforming [PWTO], and PWTO after heterodyning [PWTO*]) and regularisation (unregularised RF tracking, combined envelope/RF tracking without regularisation, and combined envelope/RF tracking with explicit regularisation) to the performance of US displacement estimation algorithms. More specifically, a non-rigid image registration technique was used to track lateral tissue motion in an in-silico and in-vitro phantom setup for all beamforming-tracking combinations. It was found that PWTO and PWTO* tracking benefited more from regularisation than FOC tracking, and was even a necessary requirement to warrant the use of TO beamforming over traditional FOC imaging. For example, for a range of lateral displacements (0–1000 µm), in-silico errors were 56±56 µm and 118±293 µm for the unregularized FOC and PWTO* scenario respectively. After regularisation, these errors remained relatively stable for the FOC case but drastically reduced for the PWTO* scenario: 51±45 µm versus 14±12 µm respectively.
A Stochastic Catastrophe Model of Knowledge Sharing Within a Team Including Simulations

An evolutionary game model on knowledge sharing within a team is developed,in which oversight mechanism and award mechanism are introduced. The Gaussian White noise is introduced to show the disturbance in this game. The catastrophe of sharing behavior,emerging from the continuous changes of the parameters about motivation mechanisms,the natures of members and the interactions between them,is analyzed by means of stochastic catastrophe theory( SCT). The catastrophe set of control variables is found to explain and forecast the catastrophe of strategic alliances. In the evolutionary process,there are several catastrophe characteristics:bimodality that implies the sensitivity to external disturbance; sudden jumps that imply the sensitivity to the continuous changes of the parameters; hysterisis that reflects the fact that it is more reliable to maintain sharing knowledge than to administer non-sharing knowledge.
How novel methods can help discover more information about foodborne pathogens.

Considerable emphasis is being placed on quantitative risk assessment modelling as a basis for regulation of trade in food products. However, for models to be accurate, information about the behaviour of potential pathogens in foods needs to be available. The question is how to obtain this knowledge in a simple and cost effective way. One technique that has great potential is the use of reporter bacteria which have been genetically modified to express a phenotype that can be easily monitored, such as light production in luminescent organisms. Bacteria carrying these (lux) genes can easily be detected using simple luminometers or more sophisticated low light imaging equipment.By monitoring light output from these bacteria over time, it can easily be determined if the organism is growing (resulting in an increase in light emission), is dead (causing a decrease in light production) or is injured (light output remains constant). The use of imaging systems allows the response of bioluminescent bacteria to be studied directly on the food, making the technique even more useful. Applications of bioluminescence are discussed below and include use as reporters of gene expression; biocide efficacy and antibiotic susceptibility; sub-lethal injury; adhesion and biofilm formation; the microbial ecology of foods; pathogenesis; and as biosensors.
PaintBoard: prototyping interactive character behaviors by digitally painting storyboards

The creation of interactive computer-controlled characters in interactive media is a challenging and multi-faceted task requiring the skills and effort of professionals from many fields. This work addresses authoring the interactive aspect of these characters' behaviors -- how characters act automatically in response to a dynamic user-controlled character. We present PaintBoard, a system that enables users to prototype and test discrete, real-time, interactive movements in a 2D grid environment simply by digitally painting a storyboard. We designed and developed a novel authoring technique for creating behaviors (painting storyboards) and a novel algorithm based on machine-learning, that analyzes a storyboard to create a behavior that works beyond situations provided in the input storyboard. We conducted two exploratory studies that grounded the prototype design, and present the results of a proof-of-concept workshop with game developers. Finally, we performed a comparison of machine learning algorithms' performance on our storyboard data.
Effect of PEG-superoxide dismutase on the diaphragmatic response to endotoxin.

Although it is known that endotoxin can induce diaphragmatic dysfunction, the mechanism of this effect is not fully understood. However, because the effects of endotoxin on other tissues appear to be mediated in part by free radicals, the present study sought to determine if free radicals may also contribute to the diaphragmatic dysfunction induced by endotoxin administration. Studies were performed on four groups of hamsters. One group of animals received intraperitoneal injections of endotoxin on the first and second days of study (i.e., 10 and 20 mg/kg, respectively). The second group received saline rather than endotoxin, the third group received both endotoxin and a free radical scavenger, PEG-SOD (2,000 U/kg given intraperitoneally every 12 h on Days 1 and 2), and the fourth group received PEG-SOD alone. All groups were killed on the third study day (i.e., 48 h after the initial injections). Diaphragmatic contractile function was assessed in vitro using muscle strips excised from the costal diaphragms of freshly killed animals; diaphragm samples were also assayed for malondialdehyde (MDA), a commonly used index of free-radical-mediated lipid peroxidation. MDA levels were higher in diaphragms from endotoxin-treated animals than from saline-treated control animals, and the contractility of diaphragm strips from endotoxin-treated animals was reduced when compared with strips from saline-treated control animals. Administration of PEG-SOD prevented MDA formation and contractile dysfunction in endotoxin-treated animals. Diaphragm contractility and MDA levels for animals given PEG-SOD alone were similar to those for saline-treated control animals. (ABSTRACT TRUNCATED AT 250 WORDS)
Prolongation of Survival in Metastatic Melanoma After Active Specific Immunotherapy With a New Polyvalent Melanoma Vaccine

A new polyvalent melanoma cell vaccine (MCV) was administered to 136 stage. IIIA and IV (American Joint Committee on Cancer) melanoma patients. Induction of cell-mediated and humoral immune responses to common melanoma-associated antigens present on autologous melanoma cells was observed in patients receiving the new MCV. This was accompanied by increased activation of tumor-infiltrating lymphocytes. Survival correlated significantly with delayed cutaneous hypersensitivity (p = 0.0066) and antibody responses to MCV (p = 0.0117). Of 40 patients with evaluable disease, nine (23%) had regressions (three complete). From our historical database of 126 stage IIIA and 1275 stage IV melanoma patients, there were no significant changes in the natural history of metastatic melanoma during the past 20 years. Univariate and multivariate analyses demonstrated prognostic significance for site of metastases (p = 0.0001) and immunotherapy with the new MCV (p = 0.0001). Overall our new MCV increased the median and 5-year survival of stage IIIA melanoma patients with regional soft tissue metastases twofold (p = 0.00024), and stage IV patients threefold (p = 0.0001) compared with previous immunotherapy and other treatments.
Synergy Between Tissue Factor and Exogenous Factor XIa in Initiating Coagulation

Objective—Recent evidence suggests involvement of coagulation factor XIa (FXIa) in thrombotic event development. This study was conducted to explore possible synergies between tissue factor (TF) and exogenous FXIa (E-FXIa) in thrombin generation. Approach and Results—In thrombin generation assays, for increasing concentrations of E-FXIa with low, but not with high TF concentrations, peak thrombin significantly increased whereas lag time and time to peak significantly decreased. Similar dependencies of lag times and rates of thrombin generation were found in mathematical model simulations. In both in vitro and in silico experiments that included E-FXIa, thrombin bursts were seen for TF levels much lower than those required without E-FXIa. For in silico thrombin bursts initiated by the synergistic action of TF and E-FXIa, the mechanisms leading to the burst differed substantially from those for bursts initiated by high TF alone. For the synergistic case, sustained activation of platelet-bound FIX by E-FXIa, along with the feedback-enhanced activation of platelet-bound FVIIIa and FXa, was needed to elicit a thrombin burst. Furthermore, the initiation of thrombin bursts by high TF levels relied on different platelet FIX/FIXa binding sites than those involved in bursts initiated by low TF levels with E-FXIa. Conclusions—Low concentrations of TF and exogenous FXIa, each too low to elicit a burst in thrombin production alone, act synergistically when in combination to cause substantial thrombin production. The observation about FIX/FIXa binding sites may have therapeutic implications.
Anti‐neutrophil cytoplasmic antibodies directed against the bactericidal/permeability‐increasing protein (BPI) in pediatric cystic fibrosis patients do not recognize N‐terminal regions important for the anti‐microbial and lipopolysaccharide‐binding activity of BPI

This study was performed to examine the prevalence and clinical correlates of bactericidal/permeability‐increasing protein anti‐neutrophil cytoplasmic antibodies (BPI‐ANCA) in pediatric cystic fibrosis (CF) patients and to elucidate their possible role in CF pulmonary pathology. Sera of 27 CF patients were tested for ANCA by indirect immunofluorescence (IFT) and by enzyme‐linked immunosorbent assay (ELISA) for ANCA sub‐specificities. BPI‐ANCA were examined by using standard ELISA for BPI, lipopolysaccharide‐binding protein (LBP), and BPI/LBP fusion proteins to epitope map the main binding sites and look for cross‐reactivity with LBP. Pulmonary function and serum concentrations of total immunoglobulin G (IgG) were measured and infections were diagnosed. In addition, release of reactive oxygen species (ROS) by neutrophil granulocytes was measured after stimulation with monoclonal BPI‐ANCA. Using IFT, two patients showed atypical ANCA staining, six patients exhibited perinuclear ANCA staining, and no cytoplasmic ANCA staining was detected. Of 27 patients, 13 (48%) were BPI‐ANCA (IgG) positive, and three were also immunoglobulin A (IgA) BPI‐ANCA positive; one patient had ANCA against lactoferrin; and no proteinase 3 ANCA was detected in any of the patients. All BPI‐ANCA bound to the C‐terminal region of the molecule; none bound to the N‐terminus or to LBP. There was no significant correlation between clinical data and the occurence of BPI‐ANCA in this cross‐sectional study. Release of ROS from granulocytes was induced by monoclonal BPI‐ANCA. Activation of neutrophils and possible modulation of BPI‐mediated opsonophagocytosis and disposal of Gram‐negative bacteria and lipopolysaccharides by BPI‐ANCA raise the possibility that they contribute to pulmonary pathology in pediatric CF patients but intervention longitudinal studies in large groups of patients are needed to establish a causative association.
Genetic Manipulation of Neisseria gonorrhoeae

The sexually‐transmitted pathogen, Neisseria gonorrhoeae, undergoes natural transformation at high frequency. This property has led to the rapid dissemination of antibiotic resistance markers and to the panmictic structure of the gonococcal population. However, high frequency transformation also makes N. gonorrhoeae one of the easiest bacterial species to manipulate genetically in the laboratory. Techniques have been developed that result in transformation frequencies >50%, allowing the identification of mutants by screening and without selection. Constructs have been created to take advantage of this high frequency transformation, facilitating genetic mutation, complementation, and heterologous gene expression. Techniques are described for genetic manipulation of N. gonorrhoeae, as well as for growth of this fastidious organism.
COERCION AND DRUG TREATMENT FOR POSTPARTUM WOMEN

This research examined the extent to which various indicators of coercion were related to treatment retention in a gender-specific treatment program and a traditional outpatient program for pregnant and postpartum women who were mandated to enter treatment. Women who were given custody of their infant stayed in treatment longer than women who did not have custody. There was an interaction effect with women who had custody and were in the intensive day treatment program completing treatment at a much higher rate than those in the traditional program. Implications for social work professionals and policy makers are discussed.
Anomaly target detection algorithm based on JPEG images

This paper describes an anomaly target detection algorithm based on JPEG images. The algorithm deals directly with JPEG image data eliminating JPEG image decoding process. The algorithm extracts the JPEG DC parameters, and then compares two frames of adjacent DC parameters to determine whether the abnormal targets appear. Compared to the YUV image data processing methods, it costs less storage space, access time and computation, which is more suitable for embedded systems with limited resources. Finally, the simulation results of the algorithm are given.
Ultrasound‐Targeted Microbubble Destruction Enhances Gene Expression of microRNA‐21 in Swine Heart via Intracoronary Delivery

Ultrasound‐targeted microbubble destruction (UTMD) has proved to be a promising method for gene delivery. However, the feasibility and efficacy of UTMD‐mediated gene delivery to the heart of large animals remain unclear. The present study was to explore the probability of increasing the transfection of microRNA‐21 (miR‐21) in swine heart by UTMD, and to search for the most suitable transfection conditions.
Phenotyping Renal Leukocyte Subsets by Four-Color Flow Cytometry: Characterization of Chemokine Receptor Expression

To investigate mechanisms of cell-mediated injury in renal inflammatory disease it is critical to determine the surface phenotype of infiltrating renal leukocyte subsets. However, the cell-specific expression of many leukocyte receptors is difficult to characterize in vivo. Here, we report a protocol based on flow cytometry that allows simultaneous characterization of surface receptor expression on different subsets of infiltrating renal leukocytes. The described technique combines an adapted method to prepare single cell suspensions from whole kidneys with subsequent four-color flow cytometry. We recently applied this technique to determine the differential expression of murine chemokine receptors CCR2 and CCR5 on infiltrating renal leukocyte subsets. In this article, we summarize our current findings on the validity of the method as compared with immunohistology and in situ hybridization in two murine models of nonimmune (obstructive nephropathy) and immune-mediated (lupus nephritis) inflammatory renal disease. Flow cytometry analysis revealed an accumulation of CCR5-, but not CCR2-positive lymphocytes in inflamed kidneys, compared to the peripheral blood. Particularly renal CD8+ cells expressed CCR5 (79% in obstructed kidneys, 90% in lupus nephritis). In both models, infiltrating renal macrophages were positive for CCR2 and CCR5. These data corresponded to immunohistological and in situ hybridization results. They demonstrate that flow cytometric analysis of single cell suspensions prepared from inflamed kidneys is a rapid and reliable technique to characterize and quantify surface receptor expression on infiltrating renal leukocyte subsets.
The 1239G/C polymorphism in exon 5 of BACE1 gene may be associated with sporadic Alzheimer's disease in Chinese Hans

Beta‐site amyloid‐precursor protein cleaving enzyme (BACE1) is a candidate risk factor for Alzheimer's disease (AD) because of involving in generating β‐amyloid peptide, which is thought to play a central role in the pathogenesis of the disease. A single nucleotide polymorphism 1239G/C in exon 5 of BACE1 gene and a weak association between this polymorphism and AD in Caucasian APOEε4 allele carriers has been reported. To examine possible association of the polymorphism with sporadic AD, two Chinese Han cohorts including 257 patients and 242 age‐matched controls in Guangzhou and 112 patients and 113 controls in Chengdu were genotyped using PCR‐RFLP techniques. The frequency of the C allele in controls of both cohorts was 0.65, which was higher than that in Caucasian populations [0.39 by Nowotny et al. 2001: Neuroport 12:1799–1802; 0.44 by Nicolaou et al. 2001: Neurogenetics 3:203–206]. There was a significant excess of C allele among the patients in both cohorts (Guangzhou, 0.71 vs. 0.65, χ2 = 5.20, P = 0.02; Chengdu, 0.74 vs. 0.65, χ2 = 4.36, P = 0.04). The CC genotype was found to be associated with AD (Guangzhou cohort, OR = 1.56, 95% CI = 1.09–2.23; Chengdu cohort, OR = 1.74, 95% CI = 1.03–2.95; combined sample: OR = 1.61, 95% CI = 1.20–2.17). The association remained in non‐APOE ε4 allele carriers when all subjects were divided on the basis of the APOEε4 status. Our findings suggest that the 1239G/C polymorphism in exon 5 of BACE1 gene may be associated with sporadic AD in Chinese Hans. © 2003 Wiley‐Liss, Inc.
Allergic Sensitization and Disease in Mother-Child Pairs from Germany: Role of Early Childhood Environment

Background: Early childhood influences are important for the development of the allergic phenotype. In East Germany, tremendous lifestyle changes took place after 1990 and it can be hypothesized that the allergic phenotypes in mothers and their children are less similar than in West Germany. This was investigated in our study done in mothers and their 6-year-old children from East and West Germany in the year 2000. Methods: 1,393 mother-child pairs participated. A subgroup of 774 pairs gave blood for the determination of specific IgE. Regional differences in mother-child correlations and in prevalence of mother-child combinations with respect to allergic sensitization and disease were examined by logistic regression analysis. Results: The adjusted association in positive allergic sensitization between mothers and their children was not significant in East Germany (OR 1.23, 95% CI: 0.68–2.24) but highly significant in West Germany (OR 2.89, 95% CI: 1.73–4.80). The probability for the combination of ‘negative’ mother and ‘positive’ child was significantly higher in East than in West Germany. Conclusions: Mother-child transmission of atopy predisposition can even be cancelled by environmental changes.