Miracle-dz/UniFAR-FTUs huggingface dataset:
Down‐regulation of Cytotoxic T Lymphocyte Generation by Two Distinct Suppressor‐Cell Systems a

Two distinct suppressor systems have been described that are capable of down-regulating in vivo generation of cytotoxic T cells directed toward haptenaltered self-antigens. One system, induced by hapten, involves three T cells that others have shown to function sequentially to suppress DTH. The initiator of this cascade is a T cell that is readily induced in spleens of mice injected intravenously with syngenic membrane-coupled hapten. This Ts, when triggered by the same syngeneic membrane-coupled hapten that induced it, elaborates a factor. The other two Ts arise in lymph nodes and spleens of mice painted epidermally with hapten. One of the two Ts in this set is readily armed by the factor of the first Ts. The factor confers its specificity and genetic restriction upon the accepting Ts. The latter, when properly triggered, makes a factor that is taken up by its companion Ts, which actually suppresses by way of a nonspecific factor. Whereas this Ts cascade is operative at the efferent limb of DTH, it mediates suppression only at the afferent phase of the CTL response. A distinctly different suppressor system is induced by minor locus (Mls) antigen. When Mlsd lymphoid cells are injected intravenously into Mlsc-possessing mice, an Lyt-1+ T-suppressor cell is generated that can be found in the spleen as well as among peritoneal exudate cells. This Ts interacts with macrophages to accomplish nonspecific suppression of the CTL response that is detectable both in vivo as well as in vitro. A Ts soluble product has been found to be effective to suppress CTL generation in vitro only when macrophages are present in culture. The macrophage that accomplishes suppression is I-A-. Although the afferent limb of the CTL response is down-regulated by this suppressor system, our in vitro culturing system is so structured as to make the helper T cell inactive. Thus, the mechanism of suppression must be oriented to the other early participants in the response, namely, precursor CTL, helper and differentiation factors, and/or the antigen-presenting cell.
Low-dose efficacy of botulinum toxin A for axillary hyperhidrosis: a randomized, side-by-side, open-label study.

OBJECTIVE To compare 2 doses of botulinum toxin A in view of dose-dependent efficacy, longevity, and safety. DESIGN Side-by-side, controlled, randomized clinical trial with extended follow-up over 2 treatment cycles. Patients were injected with 200 U of botulinum toxin A (Dysport; Ipsen Ltd, Wrexham, England) into one axilla and 100 U into the other axilla in a randomized fashion. After 48 weeks of follow-up, the patients were given a second treatment with identical doses to the respective axillae and were again followed up for 48 weeks. Gravimetric measurements of sweat production and the patients' own rating of sweating were monitored. SETTING University-based outpatient clinic. PATIENTS Forty-three subjects with primary axillary hyperhidrosis that was unresponsive to topical therapy. MAIN OUTCOME MEASURE Absolute values of sweat production. RESULTS Two weeks after treatment, the sweat production was significantly reduced compared with baseline levels. Both doses were equally effective. At week 48, the sweat production had returned to baseline levels irrespective of the dose. After the second treatment, both doses were again equally effective at any follow-up point. At the end of the follow-up period (96 weeks) for the second treatment, the sweat production was significantly lower than at the end of the first follow-up period (48 weeks). The treatment was well tolerated, and there were no lasting or severe adverse effects. CONCLUSIONS Short- and long-term results show that doses of 100 and 200 U of botulinum toxin A are equally safe and effective. However, because of cost considerations and possible adverse effects, the lower dose is preferable for treating axillary hyperhidrosis.
The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation

In this study, we have identified a unique combinatorial effect of the chemokines KC/MIP-2 and the cytokine granulocyte colony-stimulating factor (G-CSF) with respect to the rapid mobilization of neutrophils from the bone marrow in a model of acute peritonitis. At 2 hours following an intraperitoneal injection of thioglycollate, there was a 4.5-fold increase in blood neutrophil numbers, which was inhibited 84% and 72% by prior administration of blocking mAbs against either the chemokines KC/MIP-2 or G-CSF, respectively. An intraperitoneal injection of G-CSF acted remotely to stimulate neutrophil mobilization, but did not elicit recruitment into the peritoneum. Further, in vitro G-CSF was neither chemotactic nor chemokinetic for murine neutrophils, and had no priming effect on chemotaxis stimulated by chemokines. Here, we show that, in vitro and in vivo, G-CSF induces neutrophil mobilization by disrupting their SDF-1α–mediated retention in the bone marrow. Using an in situ perfusion system of the mouse femoral bone marrow to directly assess mobilization, KC and G-CSF mobilized 6.8 × 106 and 5.4 × 106 neutrophils, respectively, while the infusion of KC and G-CSF together mobilized 19.5 × 106 neutrophils, indicating that these factors act cooperatively with respect to neutrophil mobilization.
QRS complex processing system for telemetry — A comparative study based on filters

ECG records the electrical activity of the heart and is used to diagnose various cardiac disorders. One of the main targets of ECG analysis is the QRS complex that represents the excitation of the ventricles, the major pumps of the body. Therefore, accurate and reliable acquisition, processing and analysis of QRS complex is essential. Several filtering methods have been suggested for removal of noise such as baseline wandering and power line interference. Furthermore, various data compression schemes are available for ECG storage and telemetry applications such as Discrete Cosine Transform (DCT) and Discrete Wavelet Transform (DWT). While the quality of reconstructed signal and compression ratio are poor in DCT, implementation of DWT is complex. Recently a new method for QRS complex compression and reconstruction had been proposed based on the Hermite polynomials that offers high compression ratio with less approximation error. In this work, three types of filtering methods namely median, Butterworth and using wavelet decomposition were applied on the QRS complex for preprocessing. Each of these preprocessed signals were compressed using Hermite functions. Comparing the quality of reconstructed QRS complex, we found that wavelet decomposition method offered a high quality reconstructed signal but at the cost of increased complexity of implementation while the signal quality was comparable in median and Butterworth filtering methods.
Cyclosporin A ameliorates mitochondrial ultrastructural injury in the ileum during acute endotoxemia*

ObjectiveThis study was designed to determine the role, if any, of the mitochondrial permeability transition in the pathogenesis of mitochondrial injury in a representative systemic organ during the acute phase of endotoxemia. DesignA well-established, normotensive feline model was employed to determine whether pretreatment with cyclosporin A, a potent inhibitor of the mitochondrial permeability transition, reduces the severity of mitochondrial injury in the ileum during acute endotoxemia. SettingThe Ohio State University Medical Center research laboratory. SubjectsAdult, male conditioned cats. InterventionsVolume resuscitation and maintenance of acid/base balance and tissue oxygen availability were provided, as needed, to minimize the potentially confounding effects of tissue hypoxia and/or acidosis on the experimental results. Following isotonic saline vehicle (control; n = 6), lipopolysaccharide (3.0 mg/kg, intravenously; n = 10), or cyclosporin A (6 mg/kg, intravenously; n = 7) followed in 60 mins by lipopolysaccharide (3.0 mg/kg, intravenously) administration, ileal samples were obtained at 4 hrs posttreatment, and mitochondrial ultrastructure was assessed. Objective comparisons of mitochondrial ultrastructural morphology were performed by using digital image analyses. Measurements and Main ResultsAs expected, significant mitochondrial injury was apparent in the ileal tissues by 4 hrs following LPS treatment, despite maintenance of regional tissue oxygen availability. Objective evaluation of mitochondrial morphology demonstrated characteristics consistent with high-amplitude swelling. Cyclosporin A pretreatment protected against the development of these LPS-induced mitochondrial ultrastructural abnormalities, an effect not attributable to the suppression of lipopolysaccharide-induced tumor necrosis factor-&agr; production. ConclusionsThese investigations are the first to demonstrate a protective effect of cyclosporin A against mitochondrial injury in a representative systemic organ during acute endotoxemia. We propose that mitochondrial injury likely related to induction of the mitochondrial permeability transition may participate in the pathogenesis of systemic organ injury and organ failures during acute sepsis.
Biological Factors Contributing to the Response to Cognitive Training in Mild Cognitive Impairment

In mild cognitive impairment (MCI), small benefits from cognitive training were observed for memory functions but there appears to be great variability in the response to treatment. Our study aimed to improve the characterization and selection of those participants who will benefit from cognitive intervention. We evaluated the predictive value of disease-specific biological factors for the outcome after cognitive training in MCI (n = 25) and also considered motivation of the participants. We compared the results of the cognitive intervention group with two independent control groups of MCI patients (local memory clinic, n = 20; ADNI cohort, n = 302). The primary outcome measure was episodic memory as measured by verbal delayed recall of a 10-word list. Episodic memory remained stable after treatment and slightly increased 6 months after the intervention. In contrast, in MCI patients who did not receive an intervention, episodic memory significantly decreased during the same time interval. A larger left entorhinal cortex predicted more improvement in episodic memory after treatment and so did higher levels of motivation. Adding disease-specific biological factors significantly improved the prediction of training-related change compared to a model based simply on age and baseline performance. Bootstrapping with resampling (n = 1000) verified the stability of our finding. Cognitive training might be particularly helpful in individuals with a bigger left entorhinal cortex as individuals who did not benefit from intervention showed 17% less volume in this area. When extended to alternative treatment options, stratification based on disease-specific biological factors is a useful step towards individualized medicine.
A Two-Component Regulator Mediates Population-Density-Dependent Expression of the Bradyrhizobium japonicum Nodulation Genes

ABSTRACT Bradyrhizobium japonicum nod gene expression was previously shown to be population density dependent. Induction of the nod genes is highest at low culture density and repressed at high population densities. This repression involves both NolA and NodD2 and is mediated by an extracellular factor found in B. japonicum conditioned medium. NolA and NodD2 expression is maximal at high population densities. We demonstrate here that a response regulator, encoded by nwsB, is required for the full expression of the B. japonicum nodYABC operon. In addition, NwsB is also required for the population-density-dependent expression of both nolA and nodD2. Expression of nolA and nodD2 in the nwsB mutant remained at a basal level, even at high culture densities. The nwsB defect could be complemented by overexpression of a second response regulator, NodW. Consistent with the fact that NolA and NodD2 repress nod gene expression, the expression of a nodY-lacZ fusion in the nwsB mutant was unaffected by culture density. In plant assays with GUS fusions, nodules infected with the wild type showed no nodY-GUS expression. In contrast, nodY-GUS expression was not repressed in nodules infected with the nwsB mutant. Nodule competition assays between the wild type and the nwsB mutant revealed that the addition of conditioned medium resulted in a competitive advantage for the nwsB mutant.
Object recognition and localization

Aim of this project is to detect and recognize a particular object in a video and then find out corresponding timing details of that object present in the video sequence, i.e, what are frames that object is available or the different time interval this object is available in the video scene. we proposed an algorithm which will take care of the object recognition and localization in a video. Object detection and classification are the major part of the object recognition. Object detection is carried out with the help of GMM and object localization using method of object proposal calculation. also we have used Bag of word model to training data set.
Using partition designs to enhance purification process understanding

Characterization of purification processes by identifying significant input parameters and establishing predictive models is vital to developing robust processes. Current experimental design approaches restrict analysis to one process step at a time, which can severely limit the ability to identify interactions between process steps. This can be overcome by the use of partition designs which can model multiple, sequential process steps simultaneously. This paper presents the application of partition designs to a monoclonal antibody purification process. Three sequential purification steps were modeled using both traditional experimental designs and partition designs and the results compared as a proof of concept study. The partition and traditional design approaches identified the same input parameters within each process step that significantly affected the product quality output examined. The partition design also identified significant interactions between input parameters across process steps that could not be uncovered by the traditional approach. Biotechnol. Bioeng. 2010;107: 814–824. © 2010 Wiley Periodicals, Inc.
Time-Use Analytics: An Improved Way of Understanding Gendered Agriculture-Nutrition Pathways

ABSTRACT There is a resurgence of interest in time-use research driven, inter alia, by the desire to understand if development interventions, especially when targeted to women, lead to time constraints by increasing work burdens. This has become a primary concern in agriculture-nutrition research. But are time-use data useful to explore agriculture-nutrition pathways? This study develops a conceptual framework of the micro-level linkages between agriculture, gendered time use, and nutrition and analyzes how time use has been conceptualized, operationalized, and interpreted in agriculture-nutrition literature on low- and middle-income countries (LMICs). The paper argues that better metrics, but also conceptualizations and analytics of time use, are needed to understand gendered trade-offs in agriculture-nutrition pathways. In particular, the potential unintended consequences can be grasped only if the analysis of time use shifts from being descriptive to a more theoretical and analytical understanding of time constraints, their trade-offs, and resulting changes in activity.
MicroRNA-29c Is a Signature MicroRNA under High Glucose Conditions That Targets Sprouty Homolog 1, and Its in Vivo Knockdown Prevents Progression of Diabetic Nephropathy*

Although several recent publications have suggested that microRNAs contribute to the pathogenesis of diabetic nephropathy, the role of miRNAs in vivo still remains poorly understood. Using an integrated in vitro and in vivo comparative miRNA expression array, we identified miR-29c as a signature miRNA in the diabetic environment. We validated our profiling array data by examining miR-29c expression in the kidney glomeruli obtained from db/db mice in vivo and in kidney microvascular endothelial cells and podocytes treated with high glucose in vitro. Functionally, we found that miR-29c induces cell apoptosis and increases extracellular matrix protein accumulation. Indeed, forced expression of miR-29c strongly induced podocyte apoptosis. Conversely, knockdown of miR-29c prevented high glucose-induced cell apoptosis. We also identified Sprouty homolog 1 (Spry1) as a direct target of miR-29c with a nearly perfect complementarity between miR-29c and the 3′-untranslated region (UTR) of mouse Spry1. Expression of miR-29c decreased the luciferase activity of Spry1 when co-transfected with the mouse Spry1 3′-UTR reporter construct. Overexpression of miR-29c decreased the levels of Spry1 protein and promoted activation of Rho kinase. Importantly, knockdown of miR-29c by a specific antisense oligonucleotide significantly reduced albuminuria and kidney mesangial matrix accumulation in the db/db mice model in vivo. These findings identify miR-29c as a novel target in diabetic nephropathy and provide new insights into the role of miR-29c in a previously unrecognized signaling cascade involving Spry1 and Rho kinase activation.
Reasons provided by prescribers when overriding drug-drug interaction alerts.

OBJECTIVES To investigate prescribers' rationales for overriding drug-drug interaction (DDI) alerts and to determine whether these reasons were helpful to pharmacists as a part of prescription order verification. STUDY DESIGN An observational retrospective database analysis was conducted using override reasons derived from a computerized system at 6 Veterans Affairs medical centers. METHODS Data on DDI alerts (for interactions designated as "critical" and "significant") were obtained from ambulatory care pharmacy records from July 1, 2003, to June 30, 2004. Prescribers' reasons for overriding alerts were organized into 14 categories and were then rated as clinically useful or not to the pharmacist in the assessment of potential patient harm. RESULTS Of 291,890 overrides identified, 72% were for critical DDIs. Across the Veterans Affairs medical centers, only 20% of the override reasons for critical DDI alerts were rated as clinically useful for order verification. Despite a mandatory override reason for critical DDI alerts, 53% of the responses were "no reason provided. " The top response categories for critical and significant DDI alerts were "no reason provided," "patient has been taking combination," and "patient being monitored. " CONCLUSIONS When given the opportunity to provide a reason for overriding a DDI alert, prescribers rarely enter clinical justifications that are useful to order verification pharmacists. This brings into question how computerized physician order entry systems should be designed.
Food Frequency Questionnaire Is a Valid Tool for the Assessment of Dietary Habits of South Indian Pregnant Women

The food frequency questionnaire (FFQ) was validated against multiple 24-hour dietary recalls (24-HDRs) and for a few blood biomarkers in 154 pregnant women at the obstetrics and gynecology department of St John’s Medical College Hospital, Bangalore, India. Absolute nutrient intakes from the FFQ correlated positively with the average 24-HDR during pregnancy. Energy-adjusted nutrients from the FFQ in all trimesters, except proteins, carbohydrate, folate intake, and vitamin B6 in the third trimester, correlated positively with average 24-HDR. Overestimation by the FFQ compared with the 24-HDR ranged from 9% to 41%. Vitamin B12 status in the first and second trimesters positively correlated with energy-adjusted and absolute vitamin B12 intakes from the FFQ. The Bland Altman plots showed a pattern such that a trend was seen toward underreporting of intakes through the FFQ, with increasing mean intakes by the 2 methods, considering 24-HDR as the reference tool. We conclude that the FFQ is a valid tool to measure dietary intakes during pregnancy.
[Intra- and interobserver reliability of digitally photodocumented findings in wrist arthroscopy].

PURPOSE The aim of this study was to evaluate the intra- and interobserver reliability of findings in wrist arthroscopies documented by digital photography. PATIENTS AND METHODS 102 consecutive wrist arthroscopies were documented by at least 6 photographs: (1) radioscaphoidal joint with radiopalmar ligaments, (2) scapholunate ligament from radiocarpal, (3) TFCC, (4) lunotriquetral joint with ulnar recessus, (5) scapholunate, and (6) lunotriquetral joint from midcarpal. More photographs were taken of additional pathologies. Arthroscopic findings were documented in an arthroscopy form (synovitis: no/yes, articular effusion: no/yes, cartilage lesion: no/yes). Lesions of the scapholunate ligament were classified according to Geissler, scapholunate and lunotriquetral ligament lesions were graded as partial or complete, TFCC lesions were categorised according to Palmer and trampoline effects noted. Three months later the 6 standard photos and then all photos were re-evaluated by the scoping surgeon and two experienced hand surgeons. Cohen's kappa was used as a measure of agreement between findings three months postoperatively and those in the operation report. Further statistical analyses were done, e. g. , to compare intra- and interobserver reliability. RESULTS In general intraobserver reliability (mean kappa 0.44) was better than interobserver reliability (0.28, 0.30). Assessing all available photos (0.47) did not improve reliability compared to assessing only the six standard photos (0.44). On grading scapholunate ligament lesions as partial or complete the interobserver reliability was fair (0.37, 0.30). Using Geissler's classification the interobserver reliability was κ=0.34 and 0.23. There was substantial intra- (0.61) and interobserver reliability (0.63, 0.60) for cartilage lesions. While intraobserver reliability for TFCC lesions was substantial (0.61), interobserver reliability was only moderate (0.31, 0.48). CONCLUSION Even with wrist arthroscopy, the best diagnostic modality for scapholunate ligament, TFCC and carpal cartilage lesions, hand surgeons do not agree in diagnosis and pathology. Therefore, the documentation of arthroscopic findings has to be improved. Digital video documentation of wrist arthroscopy may illustrate wrist pathologies more adequately. However, this still has to be proven by further studies.