Miracle-dz/UniFAR-FTUs huggingface dataset:
T antigen and template requirements for SV40 DNA replication in vitro.

A cell‐free system for replication of SV40 DNA was used to assess the effect of mutations altering either the SV40 origin of DNA replication or the virus‐encoded large tumor (T) antigen. Plasmid DNAs containing various portions of the SV40 genome that surround the origin of DNA replication support efficient DNA synthesis in vitro and in vivo. Deletion of DNA sequences adjacent to the binding sites for T antigen either reduce or prevent DNA synthesis. This analysis shows that sequences that had been previously defined by studies in vivo to constitute the minimal core origin sequences are also necessary for DNA synthesis in vitro. Five mutant T antigens containing amino acid substitutions that affect SV40 replication have been purified and their in vitro properties compared with the purified wild‐type protein. One protein is completely defective in the ATPase activity of T antigen, but still binds to the origin sequences. Three altered proteins are defective in their ability to bind to origin DNA, but retain ATPase activity. Finally, one of the altered T antigens binds to origin sequences and contains ATPase activity and thus appears like wild‐type for these functions. All five proteins fail to support SV40 DNA replication in vitro. Interestingly, in mixing experiments, all five proteins efficiently compete with the wild‐type protein and reduce the amount of DNA replication. These data suggest that an additional function of T antigen other than origin binding or ATPase activity, is required for initiation of DNA replication.
A Typology of Near-Identity Relations for Coreference (NIDENT)

The task of coreference resolution requires people or systems to decide when two referring expressions refer to the 'same' entity or event. In real text, this is often a difficult decision because identity is never adequately defined, leading to contradictory treatment of cases in previous work. This paper introduces the concept of 'near-identity', a middle ground category between identity and non-identity, to handle such cases systematically. We present a typology of Near-Identity Relations (NIDENT) that includes fifteen types―grouped under four main families―that capture a wide range of ways in which (near-)coreference relations hold between discourse entities. We validate the theoretical model by annotating a small sample of real data and showing that inter-annotator agreement is high enough for stability (K=0.58, and up to K=0.65 and K=0.84 when leaving out one and two outliers, respectively). This work enables subsequent creation of the first internally consistent language resource of this type through larger annotation efforts.
Continuous Clustering of Moving Objects

This paper considers the problem of efficiently maintaining a clustering of a dynamic set of data points that move continuously in two-dimensional Euclidean space. This problem has received little attention and introduces new challenges to clustering. The paper proposes a new scheme that is capable of incrementally clustering moving objects. This proposal employs a notion of object dissimilarity that considers object movement across a period of time, and it employs clustering features that can be maintained efficiently in incremental fashion. In the proposed scheme, a quality measure for incremental clusters is used for identifying clusters that are not compact enough after certain insertions and deletions. An extensive experimental study shows that the new scheme performs significantly faster than traditional ones that frequently rebuild clusters. The study also shows that the new scheme is effective in preserving the quality of moving-object clusters.
Extracorporeal membrane oxygenation for adult respiratory distress syndrome in trauma patients: A case series and systematic literature review

BACKGROUND Venovenous extracorporeal membrane oxygenation (vv-ECMO) is an established salvage therapy for severe respiratory failure, and may provide an alternative form of treatment for trauma-induced adult respiratory distress syndrome (ARDS) when conventional treatments have failed. The need for systemic anticoagulation is a relative contraindication for patients with bleeding risks, especially in multitraumatic injury. METHODS We describe a case series of four trauma patients with ARDS who were managed with ECMO admitted to the neuro critical care unit at Addenbrooke’s Hospital, Cambridge (UK), from January 2000 to January 2016. We performed a systematic review of the available literature to investigate the safety and efficacy of vv-ECMO in posttraumatic ARDS, focusing on the use of different anticoagulation strategies and risk of bleeding on patients with multiple injuries. RESULTS Thirty-one patients were included. A heparin bolus was given in 16 cases. Eleven patients developed complications during treatment with ECMO with three cases of major bleeding. In all documented cases of bleeding a bolus and infusion of heparin was administered, aiming for an activated clotting time (ACT) target longer than 150 seconds. Two patients treated with heparin-free ECMO developed thromboembolic complications. Four patients died, and death was never directly or indirectly related to use of ECMO. CONCLUSION vv-ECMO can be lifesaving in respiratory failure. Our experience and our literature review suggest that vv-ECMO should be considered as a rescue treatment for the management of severe hypoxemic respiratory failure secondary to ARDS in trauma patients. For patients with a high risk of bleeding, the use of ECMO with no initial anticoagulation could be considered a valid option. For patients with a moderate risk of bleeding, use of a heparin infusion keeping an ACT target shorter than 150 seconds can be appropriate. LEVEL OF EVIDENCE Therapeutic study, level V.
Students direct inclusive school development in an Australian secondary school: An example of student empowerment

This paper reports on processes employed at a secondary state high school in Australia, where students directed inclusive school development. The procedures used in the study were developed from the Index for Inclusion and included a student forum; a student presentation to parents, principal and teachers and a focus group interview with members of the school community. These procedures were designed to empower students to participate in school review and planning and evaluate the procedures developed from the Index for Inclusion. Samples of interview data from the school principal, staff, parents and the students illustrate a growing understanding of what inclusive education means for members of this school community. The research extends understandings of inclusive education in schools, from a focus on students with disabilities to a much broader philosophy that influences school culture, policy and practice for the diversity of students at the school. Discussion about feeling part of a ‘family’ in the school community and the description of the procedures linked to actions for change, provide evidence of a developing inclusive school culture that will inform educators interested in inclusive school development.
‘A career in christian charity’: women's benevolence and the public sphere in a mid-nineteenth-century Canadian city

Abstract This article investigates the relationship between women's charitable work and the public sphere, focusing on the Hamilton Ladies Benevolent Society which operated in nineteenth-century Ontario. It argues that although women were barred from participation in the public sphere by patriarchal notions of ‘reason' and ‘independence,’ charitable associations offered political schooling wherein women internalized and problematized ‘publicness. ’ An investigation of the annual reports of the Hamilton Ladies Benevolent Society and Orphan Asylum reveals that charitable women used particular discursive tactics and techniques of display to make claims upon the public sphere. In their attempt to appear public – universal, rational, and in pursuit of an objective, common good – these women rejected the tropes of true womanhood and evoked Christian metaphors to justify their activities. For these women, Christianity provided the language with which they claimed universality, rationality and even citizenship
Comparison of Techniques for Capacity Increase in UMTS Data Services

In this paper, a comparison study of various UMTS data transmission techniques is performed. An analysis of the system in the presence of geographically non-uniform multiservice traffic profiles is done for three transmission techniques: frequency division duplex (FDD), time division duplex (TDD), and High Speed Downlink Packet Access (HSDPA). Several combinations were selected: FDD, TDD, FDD combined with HSDPA, FDD combined with TDD, and FDD combined with TDD and HSDPA. The TDD mode is the technique with better results, but the introduction of HSDPA is clearly beneficial for data services, as expected. The distribution of the services by the three techniques is the best scenario, namely for some traffic profiles. For example, for a threshold of 128 kbps (for HSDPA services) and 50 % of the power reserved to HSDPA, it is possible to get 1.4 GB of carried traffic.
Dyer Object Removal Using Exemplar-Based Inpainting

Two commonly used approaches to fill the gaps after objects are removed from digital graphics are: image inpainting and texture synthesis. Both approaches have its advantages and disadvantages. Image inpainting will cause blur, while texture synthesis will lead to loss of linear structures. Criminisi, etc., combined the two approaches to a new algorithm that utilized the advantages of both approaches. Higher filling priority will be given to where linear structures are naturally extended into the gap. With this algorithm, the gap will be filled with non-blur textures, while at the same time preserve and extend the linear structure of the surrounding area. In this report, I will first introduce the existing approaches to fill the gap. Then, I will explain Criminisi’s algorithm in detail. The third part is the discussion of my design and implementation details. Results will be presented and analyzed. Lastly, I will spend the last part of the report to discuss about the possible future directions.
Radiative processes for Rindler and accelerating observers and the stress-tensor detector

We consider a monopole detector interacting with a massive scalar field. The radiative processes are discussed from the accelerated frame point of view. After this, we obtain the Minkowski vacuum stress tensor measured by the accelerated observer using a non-gravitational stress tensor detector as discussed by Ford and Roman (PRD 48, 776 (1993)). Finally, we analyse radiative processes of the monopole detector travelling in a world line that is inertial in the infinite past and has a constant proper acceleration in the infinite future.
Alcohol craving as a predictor of relapse.

BACKGROUND AND OBJECTIVES Alcoholism treatment interventions, both psychosocial and pharmacologic, aim to reduce cravings to drink. Yet, the role of craving in treatment outcomes remains unclear. This study evaluated craving intensity measured with the Penn Alcohol Craving Scale (PACS) at admission and discharge from residential treatment as a predictive factor of relapse after treatment. METHODS The study cohort included 314 alcohol-dependent subjects. Associations between relapse after discharge, PACS score, and clinical variables were investigated using time-to-event analyses. The primary analysis, based on the intent-to-treat principle, presumed relapse in those declining follow-up or not responding to contact attempts. Secondary analysis utilized data from 226 subjects successfully contacted after discharge with a median follow-up time of 365 days. RESULTS The intent-to-treat analysis demonstrated that relapse was associated with higher level of craving at admission (p= .002) and discharge (p < .001). The analysis of data from patients successfully contacted after discharge led to similar results. A multivariable analysis indicated that relapse rates increased as PACS scores increased, and a higher discharge PACS score was significantly associated with relapse (p= .006) even after adjusting for covariates. CONCLUSIONS AND SCIENTIFIC SIGNIFICANCE This study demonstrates that higher PACS scores at the time of admission and discharge are associated with relapse following residential addiction treatment. These data support the role of craving in relapse and the utility of craving measurement as a clinical guide in assessing relapse risk.
GPU-based Power-Grasp And Placement Planners For Unknown Environments

A Personal Robot should be able to handle unknown objects in unknown environments. For a manipulation task the question of what to do with an object once it has been grasped, is one of the most essential ones besides the grasping task itself. Moreover, the planning time should be at least as fast as the time the robot needs for its grasping/placing motions. We propose a combination of a fast grasp and placement planner that deals with sensor-modelled objects. They share novel pose computation steps that reduce the amount of computational intensive collision tests. By means of experiments we evaluate the computation time for the pose computation. Furthermore, we present the qualitative results of experiments showing accurate grasps and object placements
Construction and Display Of Competence And (Professional) Identity In Coaching Interactions

This article focuses on the analysis of the display, negotiation, and construction of competence and (professional) identity in a relatively young field of personal and professional development: coaching. Based on a corpus of German and English coaching sessions, the related study utilizes a multidisciplinary approach, combining ethnomethodology, conversation analysis, membership categorization analysis, and discursive psychology. In the analysis of a first business coaching encounter between professional and client presented in this article, competence can be seen as part of the coach’s professional identity, as it is co-constructed on a moment-to-moment basis by both coach and client in and via their talk-in-interaction. While the membership categories of “coach” and “client” set the framework for the analysis, a more detailed description of the specific interactional activities of the two interactants is needed in order to get a grasp of the notions of competence and professional identity. For this analytical purpose, an extension of Zimmerman’s model of identity is suggested on the level of situated identities.
Stromal cell-derived factor-1 (SDF1)-dependent recruitment of bone marrow-derived renal endothelium-like cells in a mouse model of acute kidney injury

Ischemic acute kidney injury (AKI) is the most key pathological event for accelerating progression to chronic kidney disease through vascular endothelial injury or dysfunction. Thus, it is critical to elucidate the molecular mechanism of endothelial protection and regeneration. Emerging evidence indicates that bone marrow-derived cells (BMCs) contribute to tissue reconstitution in several types of organs post-injury, but little is known whether and how BMCs contribute to renal endothelial reconstitution, especially in an early-stage of AKI. Using a mouse model of ischemic AKI, we provide evidence that incorporation of BMCs in vascular components (such as endothelial and smooth muscle cells) becomes evident within four days after renal ischemia and reperfusion, associated with an increase in stromal cell-derived factor-1 (SDF1) in endothelium and that in CXCR4/SDF1-receptor in BMCs. Notably, anti-CXCR4 antibody decreased the numbers of infiltrated BMCs and BMC-derived endothelium-like cells, but not of BMC-derived smooth muscle cell-like cells. These results suggest that reconstitution of renal endothelium post-ischemia partially depends on a paracrine loop of SDF1-CXCR4 between resident endothelium and BMCs. Such a chemokine ligand-receptor system may be attributable for selecting a cellular lineage (s), required for renal vascular protection, repair and homeostasis, even in an earlier phase of AKI.
Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism.

INTRODUCTION. 426 Lysozyme and Penicillin. 426 Peptidoglycan in Gram-Positive Bacteria. 426 Peptidoglycan in Gram-Negative Bacteria. 427 Properties of Protoplasts and Spheroplasts. 427 Summation. 428 GENERAL STRucTURE OF THE BACTERLAL PEPTIDOGLYCAN NETWORK. 428 Glycan Strands. 428 Peptide Subunits . 428 Cross-linking Bridges . 429 ENZYMES THAT DEGRADE BACTERIAL PEProDGLycANs: NATURE OF THE HYDROLYZED LINKAGES . 430 Endo-N-Acetylmuramidases. 430 Endo-N-acetylglucosaminidases. 432 Streptomyces N-AcetylmuraMyl-L-Alanine Amidase. 432 Streptomyces KM Endopeptidase. 432 Streptomyces SA Endopeptidase. 433 Streptomyces ML Endopeptidase. 433 Myxobacter AL I Protease. 433 Streptomyces MR Endopeptidase and Lysostaphin Endopeptidase. 433 Peptidase Preparations with Mixed Activities. 433 Summation. 434 STRUCTURE OF SEVERAL BACTERIAL PEPTIDoGLycANs AS REVEALED BY ENZYMATIC DEGRADATIONS: GLYCAN MOIETY. 434 Staphylococcus aureus. 434 Micrococcus lysodeikticus . 436 Other Bacterial Peptidoglycans. 436 Molecular Size of the Glycan Moiety. 437 Base-Catalyzed Lactyl Elimination from N-Acetylmuramic Acid. 437 STRuCTuRE OF SEVERAL BACTERIAL PEPTIDOGLYCANS AS REVEALED BY ENZYMATIC DEGRADATIONS: PEPTIDE Mownr . 437 Peptidoglycans of Type I. 437 Characterization of the NH2-(i)-meso-DAP in the link to glutamic acid. 438 Characterization ofthe -y-carboxyl group ofglutamic acid in the link to NH2-(L)-mesoDAP. 439 Characterization of the link meso-DAP-(L)-(D)-Ala. 439 Characterization of the i-Ala-(D)-meso-DAP cross-linkages between peptide subunits. 439 Peptidoglycans of Type II with Peptide Bridges of Glycine or L-Amino Acid Residues, or Both . 440 Peptidoglycans of Type II with a D-Isoasparaginyl Bridge. 442 Peptidoglycans of Type III. 444 Peptidoglycans of Type IV. 446 Molecular Size of the Peptide Moieties. 446 PPTIDOGLYCAN AS A TAXONOMIC CHARACTER. 447 Gram-Negative Bacteria. 447 Actinomycetes. 447 Gram-Positive Eubacteria . 447 UNIFIED VIEW OF THE BACTERLAL PEPTDoGLycANs. 448 LYTIC ENZYMES AS A MEANS FOR THE STUDY OF THE LINK BETWEEN THE PEPTDOGLYCAN AND OTHER COMPONENTS IN WALLS OF GRAM-POSriTVE BACTERIA. 452 Micrococcus lysodeikticus. 453 Streptococcus pyogenes. 453
40Ar/39Ar thermochronology of high-pressure granulite nappes in the southern Brasília Belt, Brazil: Implications for Nappe Exhumation

Neoproterozoic orogenesis in the southern sector of the Southern Brasília Belt was the result of subduction and terminal collision between the Socorro–Guaxupé Arc and Paranapanema Block to the west (present coordinates), and the passive margin along the southern portion of the western side of the São Francisco Craton to the east. We report the results of a thermochronologic study of the uppermost passive margin derived high-pressure granulite facies nappes of the Andrelândia Nappe Complex to better constrain the timing of exhumation of these nappes. Based on 40Ar/39Ar biotite plateau ages, the Três Pontas–Varginha Nappe at the top of the Andrelândia Nappe Complex was the first nappe to be exhumed through ∼300 °C during the interval 591 to 567 Ma; this was followed by exhumation of the underlying Carmo da Cachoeira Nappe through ∼300 °C at circa 540 Ma. Initially, the Três Pontas–Varginha Nappe cooled slowly at a rate of <1°C Ma−1 from ∼800 °C at circa 648 Ma to ∼750 °C at circa 590 Ma, but then the rate of cooling increased dramatically to >20°C Ma−1. This increase is attributed to tectonically-driven exhumation during orogenic collapse triggered by regional extension as a far-field effect of eastward subduction of the passive margin on the eastern side of the São Francisco Craton during accretion of a magmatic arc terrane associated with the formation of the Ribeira Belt to the east. The average rate of cooling in the underlying Carmo da Cachoeira Nappe was 4 to 5 °C Ma−1. This slower rate reflects collapse of the nappe stack, which is likely to have led to faster exhumation of nappes higher in the stack. Exhumation of the Andrelândia Nappe through ∼300 °C circa 50 Ma after exhumation in the correlative Carmo da Cachoeira Nappe demonstrates that the two nappes were exhumed separately. The delayed exhumation of the Andrelândia Nappe may be due to reworking during orogenesis associated with formation of the Ribeira Belt. The Andrelândia Nappe records cooling from the bottom up; this was likely a consequence of final emplacement on the colder São Francisco Craton.
Children's Work and Schooling in the Late Nineteenth-Century Family Economy.

The concept of a family economy plays an important role in theory and research on the social impact of industrialization. Using family-level data from an 1890 survey of workingclass families in the U.S., we analyze the impact of the family economy on the school and work activities of children. Our analysis differentiates the family economy from the local economy and regionalfactors. Four hypotheses derivedfrom family economy theory predict the allocation of children's time to school and work. Our results lend strong empirical support for all hypotheses. In particular, we found that higher levels of family resources and lower levels of demand on those resources are associated with higher rates of children's participation in school and lower rates of children's participation in wage labor. These effects are stable after controls are introduced for family demographic composition, local economy, and region.
Electrical impedance model of piezoelectric based sensor

An electrical impedance model of a thin disc piezo based sensor, used for resonance frequency analysis, is proposed in this paper. The sensor consists of the piezo element attached to a metallic strip with some adhesive and so, the response of the sensor is dependent on the geometry and modes of vibration of both the piezo element and the metallic strip attached to it. The experimentally measured impedance responses of the piezo elements and the corresponding sensors are compared with the simulated responses to validate the proposed model. The study reveals the effects of the various aspects of the piezo and the effect of addition of the metallic strip on the sensor response in terms of various impedance blocks.
piStream: Physical Layer Informed Adaptive Video Streaming over LTE

Adaptive HTTP video streaming over LTE has been gaining popularity due to LTE's high capacity. Quality of adaptive streaming depends highly on the accuracy of client's estimation of end-to-end network bandwidth, which is challenging due to LTE link dynamics. In this paper, we present piStream, that allows a client to efficiently monitor the LTE basestation's PHY-layer resource allocation, and then map such information to an estimation of available bandwidth. Given the PHY-informed bandwidth estimation, piStream uses a probabilistic algorithm to balance video quality and the risk of stalling, taking into account the burstiness of LTE downlink traffic loads. We conduct a real-time implementation of piStream on a software-radio tethered to an LTE smartphone. Comparison with state-of-the-art adaptive streaming protocols demonstrates that piStream can effectively utilize the LTE bandwidth, achieving high video quality with minimal stalling rate.