Miracle-dz/UniFAR-FTUs huggingface dataset:
Reliability of different thresholds for defining muscular rest of the trapezius muscles in computer office workers

This study aimed at documenting the reliability of different thresholds used for defining the muscular rest of the trapezius muscles of 27 computer office workers, using surface electromyography (EMG) signals collected in the field. Measurement strategies for increasing the reliability of the results were also explored. Ten different thresholds to define muscular rest were compared: 1) five normalised (individualised) thresholds; 2) three absolute thresholds (in μV); 3) two absolute but individualised thresholds. The reliability was assessed using both a 15-min standardised computer task and 45 min of regular computer work. The main findings were: 1) overall, in a repeated measures study design, muscular rest variables were more reliable with the use of absolute thresholds when compared to normalised and individualised thresholds; 2) excellent reliability (index of dependability >0.75) can be reached when averaging the scores over 2 days; 3) using a standardised task instead of regular work does not necessarily lead to more reliable results.
Extending Recommendation Systems with Semantics and Context-Awareness: Pre-Filtering Algorithms

During the last decade, several recommendation systems have been proposed that help people to tackle information overload of digital content by effectively presenting content adapted to user’s tastes and needs. However, these personalization technologies are far from perfect and much research is needed to improve the quality of recommendations and, particularly, user satisfaction. In this paper we analyze and extend two relatively recent approaches for improving the effectiveness of recommendation systems: context-aware recommenders, which mainly focus on incorporating contextual information to the recommendation process; and semantically-enhanced recommenders, which focus on incorporating domain semantics. Although these approaches are compatible, how to properly combine them to maximize their strengths is still an unexplored research issue. The objective of this work is to provide the basis for this research. Concretely, we propose and evaluate an improved content-based model that exploits semantics and contextual information in an integrated way.
Exercise and Therapeutic Ultrasound Compared with Corticosteroid Injection for Chronic Lateral Epicondylitis: A Randomized Controlled Trial.

BACKGROUND Lateral epicondylitis, or tennis elbow, is a common overuse syndrome of the extensor tendons of the forearm. When the condition is chronic or not responding to initial treatment, physical therapy is initiated. The objective of this study is to compare the effectiveness of corticosteroid injections with physiotherapeutic interventions (ultrasound and exercise) for the treatment of chronic lateral epicondylitis. MATERIAL AND METHODS We performed a randomized controlled trial of 12 weeks' duration in patients with chronic lateral epicondylitis. We randomly assigned 49 subjects to an exercise group (n=25), who received ultrasound and exercise, and a control group (n=24), who were treated with local infiltration of 1mL triamcinolone acetonide (10mg/mL) and 1mL lidocaine 2%. To evaluate the subjects, three instruments were used: pain intensity, measured with a Visual Analogue Scale (VAS), functional disability, measured with the Patient-Rated Tennis Elbow Evaluation (PRTEE) questionnaire, and painfree grip strength. All subjects were evaluated before treatment and at the 6th and 12th week. RESULTS There were no significant differences between the two groups with regard to any variable at baseline (p>0.05). In the exercise group, significant improvements were demonstrated for VAS, PRTEE pain score, PRTEE function score and pain free grip strength, compared to the control group. The exercise group reported a significantly greater increase in all variables at 12 weeks than did the control group (p<0.001). CONCLUSION Our results suggest that ultrasound therapy and exercise are beneficial in the treatment of tennis elbow.
The influence of electrospinning parameters on polydioxanone scaffold properties

Conduits currently used to reconstruct the right ventricular outflow tract (RVOT) have no growth potential and require reoperations, resulting in an increased level of morbidity and mortality. This work investigates the effect of electrospinning parameters on the mechanical properties and biocompatibility of bioresorbable tubular scaffolds, as part of a programme to develop a tissue-engineered valved tube for RVOT replacement. Electrospinning was used to develop tubular scaffolds of polydioxanone, with the experimental parameters systematically varied. Three electrospinning parameters (volume of liquid, flow rate, and speed of mandrel rotation) were investigated, and their effects on the mechanical properties and cellular response of the scaffolds were analysed using uniaxial tensile tests, cell viability and cytotoxicity assays. Mechanical properties were compared to those of the native RVOT reported in the literature. Increasing the mandrel rotation speed tended to increase fibre alignment slightly, and an increase from 50 to 500 rpm led to more profound rises in the stress at failure and Young’s modulus. An increase in flow rate also increased the rigidity of the tubes. Cell viability and cytotoxicity assays showed all the tubes produced to have excellent biocompatibility. Through variation of the processing parameters, it is possible to tune mechanical properties of medical-grade polymer tubes over a wide range. Electrospinning therefore offers great promise in the development of scaffolds to match the properties of the native RVOT, paving the way to a future bioresorbable device to replace the RVOT in children.
Resonant nonlinear magneto-optical effects in atoms

In this article, we review the history, current status, physical mechanisms, experimental methods, and applications of nonlinear magneto-optical effects in atomic vapors. We begin by describing the pioneering work of Macaluso and Corbino over a century ago on linear magneto-optical effects (in which the properties of the medium do not depend on the light power) in the vicinity of atomic resonances, and contrast these effects with various nonlinear magneto-optical phenomena that have been studied both theoretically and experimentally since the late 1960s. In recent years, the field of nonlinear magneto-optics has experienced a revival of interest that has led to a number of developments, including the observation of ultra-narrow (1-Hz) magneto-optical resonances, applications in sensitive magnetometry, nonlinear magneto-optical tomography, and the possibility of a search for parity- and time-reversal-invariance violation in atoms.
Buckling behaviour and design of complex-shaped aluminium mullion sections

Thin-walled aluminium mullions are the vertical framing members of the façade systems used in buildings. This article investigates the buckling behaviour of these complex-shaped aluminium mullions. For this purpose, the aluminium mullion sections were simplified into elements of varying thickness and modelled using CUFSM finite strip analysis programme. Elastic buckling analyses were performed with and without considering the availability of glass panel restraints for both negative and positive wind actions, and the results are presented in this article. The effect of providing return flanges to enable a good connectivity between the male and female mullions was also evaluated. The lateral restraints provided by glass panels were simulated using the spring stiffness option available in CUFSM, and the analyses were performed for spring stiffness values in the range of 0–1 N/mm/mm. The applicability of the buckling analysis results to the design of aluminium mullions was then evaluated using the direct strength method. For this purpose, the section moment capacities of mullions were determined from finite element analyses and compared with the direct strength method predictions using the CUFSM buckling analysis results. This comparison showed that direct strength method–based design can be adopted for the complex-shaped aluminium mullions provided their elastic buckling capacities are available. Overall, this study has provided good understanding of the buckling behaviour of mullion sections under both positive and negative wind actions and has proposed the use of direct strength method for the design of aluminium mullion sections.
The prevalence and significance of a short QT interval in 18 825 low-risk individuals including athletes

Objectives The short QT syndrome is a cardiac channelopathy characterised by accelerated repolarisation which manifests as a short QT interval on the ECG. The definition of a short QT interval is debated, ranging from <390 to ≤320 ms, and its clinical significance in healthy young individuals is unknown. We assessed the prevalence and medium-term significance of an isolated short QT interval in a diverse young British population. Methods Between 2005 and 2013, 18 825 apparently healthy people aged 14–35 years underwent cardiovascular evaluation with history, physical examination and ECG. QT intervals were measured by cardiologists using 4 recommended guidelines (Seattle 2013, Heart Rhythm Society 2013, European Society of Cardiology 2010 and American Heart Association 2009). Results The prevalence of a short QT interval was 0.1% (26 patients, ≤320 ms), 0.2% (44 patients, ≤330 ms), 7.9% (1478 patients, <380 ms), 15.8% (2973 patients, <390 ms). Male gender and Afro-Caribbean ethnicity had the strongest association with short QT intervals. Athletes had shorter QT intervals than non-athletes but athletic status did not predict short QT intervals. Individuals with short QT intervals ≤320 ms did not report syncope or a sinister family history, and during a follow-up period of 5.3±1.2 years, there were no deaths in this group. Conclusions The prevalence of a short QT interval depends on the recommended cut-off value. Even at values ≤320 ms, there was an excellent medium-term prognosis among 14 people followed. We conclude that a definition of ≤320 ms is realistic to prevent overdiagnosis and excessive investigations.
Plaque Assay for Murine Norovirus

Murine norovirus (MNV) is the only member of the Norovirus genus that efficiently grows in tissue culture 1, 2. Cell lysis and cytopathic effect (CPE) are observed during MNV-1 infection of murine dendritic cells or macrophages 1. This property of MNV-1 can be used to quantify the number of infectious particles in a given sample by performing a plaque assay 1. The plaque assay relies on the ability of MNV-1 to lyse cells and to form holes in a confluent cell monolayer, which are called plaques 3. Multiple techniques can be used to detect viral infections in tissue culture, harvested tissue, clinical, and environmental samples, but not all measure the number of infectious particles (e.g. qRT-PCR). One way to quantify infectious viral particles is to perform a plaque assay 3, which will be described in detail below. A variation on the MNV plaque assay is the fluorescent focus assay, where MNV antigen is immunostained in cell monolayers 4. This assay can be faster, since viral antigen expression precedes plaque formation. It is also useful for titrating viruses unable to form plaques. However, the fluorescent focus assay requires additional resources beyond those of the plaque assay, such as antibodies and a microscope to count focus-forming units. Infectious MNV can also be quantified by determining the 50% Tissue Culture Infective Dose (TCID50) 3. This assay measures the amount of virus required to produce CPE in 50% of inoculated tissue culture cells by endpoint titration 5. However, its limit of detection is higher compared to a plaque assay 4. In this article, we describe a plaque assay protocol that can be used to effectively determine the number of infectious MNV particles present in biological or environmental samples 1, 4, 6. This method is based on the preparation of 10-fold serial dilutions of MNV-containing samples, which are used to inoculate a monolayer of permissive cells (RAW 264.7 murine macrophage cells). Virus is allowed to attach to the cell monolayer for a given period of time and then aspirated before covering cells with a mixture of agarose and cell culture media. The agar enables the spread of viral progeny to neighboring cells while limiting spread to distantly located cells. Consequently, infected cells are lysed and form holes in the monolayer known as plaques. Upon sufficient spread of virus, plaques become visible following staining of cells with dyes, like neutral red, methylene blue, or crystal violet. At low dilutions, each plaque originates from one infectious viral particle and its progeny, which spread to neighboring cells. Thus, counting the number of plaques allows one to calculate plaque-forming units (PFU) present in the undiluted sample 3.
Stoichiometry of Site-specific Lysine Acetylation in an Entire Proteome*♦

Background: Lysine acetylation sites have been mapped, but information on stoichiometry is lagging. Results: We developed and utilized the first direct, unbiased method for quantifying site-specific acetylation stoichiometry of a proteome without antibody enrichment. Conclusion: High stoichiometry is associated with central metabolism, transcription, and translation. Loss of deacetylase CobB affects site-specific and global acetylation stoichiometry, altering acetyl-CoA metabolism. Significance: Stoichiometry provides functional insight into protein acetylation. Acetylation of lysine ϵ-amino groups influences many cellular processes and has been mapped to thousands of sites across many organisms. Stoichiometric information of acetylation is essential to accurately interpret biological significance. Here, we developed and employed a novel method for directly quantifying stoichiometry of site-specific acetylation in the entire proteome of Escherichia coli. By coupling isotopic labeling and a novel pairing algorithm, our approach performs an in silico enrichment of acetyl peptides, circumventing the need for immunoenrichment. We investigated the function of the sole NAD+-dependent protein deacetylase, CobB, on both site-specific and global acetylation. We quantified 2206 peptides from 899 proteins and observed a wide distribution of acetyl stoichiometry, ranging from less than 1% up to 98%. Bioinformatic analysis revealed that metabolic enzymes, which either utilize or generate acetyl-CoA, and proteins involved in transcriptional and translational processes displayed the highest degree of acetylation. Loss of CobB led to increased global acetylation at low stoichiometry sites and induced site-specific changes at high stoichiometry sites, and biochemical analysis revealed altered acetyl-CoA metabolism. Thus, this study demonstrates that sirtuin deacetylase deficiency leads to both site-specific and global changes in protein acetylation stoichiometry, affecting central metabolism.
Clinical, Virologic, and Epidemiologic Characteristics of Dengue Outbreak, Dar es Salaam, Tanzania, 2014

We investigated a dengue outbreak in Dar es Salaam, Tanzania, in 2014, that was caused by dengue virus (DENV) serotype 2. DENV infection was present in 101 (20.9%) of 483 patients. Patient age and location of residence were associated with infection. Seven (4.0%) of 176 patients were co-infected with malaria and DENV.
Optimization of the product of nanocrystalline cellulose from coconut husks

Coconut husks can increase its value as agricultural wastes by utilizing these wastes to produce nanomaterials. Therefore, in this study prepared nanocrystalline cellulose (NCC) from coconut husks using sulphuric acid hydrolysis. The Box-Behnken Design (BBD) based on the response surface methodology was applied to study the effect of sulphuric acid concentration, reaction time and reaction temperature on the yield of NCc. The result showed that the yield of NCC was 44.94%, under optimum conditions of 64.6% concentration of sulphuric acid, 58.55 minutes and 44.56 °C. The X-ray diffraction (XRD) showed the crystallinity index of NCC was 68.3%.
Data analysis in qualitative research

Unquestionably, data analysis is the most complex and mysterious of all of the phases of a qualitative project, and the one that receives the least thoughtful discussion in the literature. For neophyte nurse researchers, many of the data collection strategies involved in a qualitative project may feel familiar and comfortable. After all, nurses have always based their clinical practice on learning as much as possible about the people they work with, and detecting commonalities and variations among and between them in order to provide individualised care. However, creating a database is not sufficient to conduct a qualitative study. In order to generate findings that transform raw data into new knowledge, a qualitative researcher must engage in active and demanding analytic processes throughout all phases of the research. Understanding these processes is therefore an important aspect not only of doing qualitative research, but also of reading, understanding, and interpreting it. For readers of qualitative studies, the language of analysis can be confusing. It is sometimes difficult to know what the researchers actually did during this phase and to understand how their findings evolved out of the data that were collected or constructed. Furthermore, in describing their processes, some authors use language that accentuates this sense of mystery and magic. For example, they may claim that their conceptual categories “emerged” from the data1—almost as if they left the raw data out overnight and awoke to find that the data analysis fairies had organised the data into a coherent new structure that explained everything! In this EBN notebook, I will try to help readers make sense of some of the assertions that are made about qualitative data analysis so that they can develop a critical eye for when an analytical claim is convincing and when it is not. Qualitative data come …
Parallel Search for Conjunctions with Stimuli in Apparent Motion

A series of experiments was conducted to determine whether apparent motion tends to follow the similarity rule (ie is attribute-specific) and to investigate the underlying mechanism. Stimulus duration thresholds were measured during a two-alternative forced-choice task in which observers detected either the location or the motion direction of target groups defined by the conjunction of size and orientation. Target element positions were randomly chosen within a nominally defined rectangular subregion of the display (target region). The target region was presented either statically (followed by a 250 ms duration mask) or dynamically, displaced by a small distance (18 min of arc) from frame to frame. In the motion display, the position of both target and background elements was changed randomly from frame to frame within the respective areas to abolish spatial correspondence over time. Stimulus duration thresholds were lower in the motion than in the static task, indicating that target detection in the dynamic condition does not rely on the explicit identification of target elements in each static frame. Increasing the distractor-to-target ratio was found to reduce detectability in the static, but not in the motion task. This indicates that the perceptual segregation of the target is effortless and parallel with motion but not with static displays. The pattern of results holds regardless of the task or search paradigm employed. The detectability in the motion condition can be improved by increasing the number of frames and/or by reducing the width of the target area. Furthermore, parallel search in the dynamic condition can be conducted with both short-range and long-range motion stimuli. Finally, apparent motion of conjunctions is insufficient on its own to support location decision and is disrupted by random visual noise. Overall, these findings show that (i) the mechanism underlying apparent motion is attribute-specific; (ii) the motion system mediates temporal integration of feature conjunctions before they are identified by the static system; and (iii) target detectability in these stimuli relies upon a nonattentive, cooperative, directionally selective motion mechanism that responds to high-level attributes (conjunction of size and orientation).
Additive Manufacturing Process and Their Applications for Green Technology

Growth of nature is an additive process that gives sustainable existence to the structures developed; on the other hand, traditional manufacturing techniques can be wasteful as they are subtractive. Additive manufacturing produces almost nil waste and accordingly preserves raw materials resulting in cost reduction for the procurement of the same. It will also cut down on the carbon emissions that are usually generated from industrial manufacturing. Additive printed objects are lighter as well, making them more efficient, especially when used in the automobile and aerospace industry. Further, the intrinsic characteristics and the promising merits of additive manufacturing process are expected to provide a solution to improve the sustainability of the process. This chapter comprehensively reports on various additive manufacturing processes and their sustainable applications for green technology. The state of the art, opportunities, and future, related to sustainable applications of additive manufacturing have been presented at length.
Geniposide Attenuates the Phosphorylation of Tau Protein in Cellular and Insulin‐deficient APP/PS1 Transgenic Mouse Model of Alzheimer's Disease

Our previous studies have shown that geniposide plays an essential role in glucose‐stimulated insulin secretion from pancreatic β cells and also regulates the metabolism of Aβ and its deposition in neurons. In this study, we reported that insulin deficiency induced significant increase of tau phosphorylation. Administration of geniposide for 4 weeks significantly decreased the phosphorylated level of tau and the acceleration of GSK‐3β phosphorylation in the brain of APP/PS1 transgenic mice induced by insulin deficiency. We also observed that geniposide decreased the phosphorylation of tau protein directly and increased the phosphorylation of Akt in primary cultured cortical neurons. Furthermore, geniposide enhanced the role of insulin on the phosphorylation of Akt, GSK‐3β, and tau in primary cultured cortical neurons. And these effects of geniposide in cortical neurons could be prevented by preincubation with LY294002, an inhibitor of PI3K. Taken together, our findings provide a mechanistic and perhaps a foundational link between diabetes and Alzheimer's disease and are consistent with the notion that geniposide might play an essential role on the phosphorylation of tau protein via enhancing insulin signaling and may convey a therapeutic benefit in Alzheimer's disease.