Miracle-dz/UniFAR-FTUs huggingface dataset:
Fas- and interferon gamma-induced apoptosis in Chang conjunctival cells: further investigations.

PURPOSE Previously interferon (IFN)gamma-induced apoptosis and expression of inflammation-related proteins in a human conjunctival cell line were demonstrated. The aim of this study was to further investigate the mechanisms of IFNgamma-, Fas-, and cycloheximide (CHX)-induced programmed cell death, with special attention to the role of transcriptional factors NF-kappaB and STAT1. METHODS In a human conjunctival cell line (Chang conjunctival cells) apoptosis was induced with 500 ng/ml anti-Fas antibody (anti-Fas ab) alone (24 or 48 hours) or, as previously reported, with 300 U/ml of human recombinant IFNgamma alone (48 hours). To study the role of IFNgamma on Fas-induced apoptosis, cells were treated first with IFNgamma at 30 U/ml during 24 hours (nontoxic dose), and then anti-Fas ab was applied for 24 hours. Moreover, to study the influence of CHX on Fas- and IFNgamma-induced apoptosis, cells were treated for 24 hours with 300 U/ml IFNgamma together with a nontoxic concentration (1 microg/ml) of CHX, or with 500 ng/ml anti-Fas ab together with 1 microg/ml CHX (24 hours). After treatment, cell viability (neutral red assay), mitochondrial membrane potential (rhodamine 123 assay), chromatin condensation (Hoechst 33342 assay), and the index Hoechst/neutral red were studied by cold light microplate cytometry. The apoptotic process was sought for by contrast phase microscopy and DAPI staining and was confirmed by immunoblotting of PARP. Activation of caspase-3 (CPP32) and caspase-8 were investigated by Western blot analysis. NF-kappaB and STAT DNA-binding activities were studied by electrophoretic mobility shift assays (EMSA). RESULTS After 24 and 48 hours of treatment with anti-Fas ab alone, 15% to 20% and 30%, respectively, of apoptotic cells were observed. When anti-Fas sera were applied after IFNgamma pretreatment or together with CHX, 50% to 80% of cells demonstrated morphologic characteristics of programmed cell death. Apoptosis was confirmed by a cleavage of PARP and CPP32, by caspase-8 activation, and by an index Hoechst/neutral red greater than one. All these modifications were preceded by a decrease in mitochondrial membrane potential. EMSA revealed that NF-kappaB was activated after IFNgamma and anti-Fas ab treatments and inhibited after CHX treatment. STAT1 was strongly activated after IFNgamma treatment and only in a minor degree after anti-Fas ab treatment. STAT1-binding activity persisted after CHX treatment. CONCLUSIONS The relative resistance of Chang cells toward Fas-induced apoptosis could be related to the activation of NF-kappaB. IFNgamma-induced programmed cell death preferentially involves the activation of STAT1 that counterbalances NF-kappaB antiapoptotic effects. In fact, Fas-induced apoptosis was potentiated by IFNgamma or CHX treatments. These results suggest that NF-kappaB activation could maintain cell viability as well as participate in IFNgamma-induced inflammatory modifications, whereas STAT1 activation could provide, in this model, a proapoptotic signal.
TELEPHONE TREATMENT OF OBSESSIVE-COMPULSIVE DISORDER

Whilst there is substantial evidence of the efficacy of exposure and response prevention in Obsessive-Compulsive Disorder (OCD), little research has focused on delivering treatment in a more cost-effective way. This study investigated the use of brief treatment of a single 45-minute face-to-face treatment session, followed by eight weekly 15-minute telephone therapy sessions, and a final face-to-face session of 30 minutes. Of the four patients included in this small pilot study, of whom all completed treatment, three clients improved and one client made slight improvement. Given these promising results, further investigation of exposure and response prevention delivered in this way is warranted.
Conflict of cultures - the beginning of a new awareness

It is widely accepted that knowledge is socially constructed (Bruffee 1986; Bazerman 1988, 1990, 1998) and that this leads to variation between the rhetorical choices and academic writing conventions adopted in different disciplinary discourses (Hyland 2000). This paper considers responses to a language awareness raising course on reading academic English, which was held in a college of agriculture in Portugal.
Cross‐modal decoupling in temporal attention

Prior studies have repeatedly reported behavioural benefits to events occurring at attended, compared to unattended, points in time. It has been suggested that, as for spatial orienting, temporal orienting of attention spreads across sensory modalities in a synergistic fashion. However, the consequences of cross‐modal temporal orienting of attention remain poorly understood. One challenge is that the passage of time leads to an increase in event predictability throughout a trial, thus making it difficult to interpret possible effects (or lack thereof). Here we used a design that avoids complete temporal predictability to investigate whether attending to a sensory modality (vision or touch) at a point in time confers beneficial access to events in the other, non‐attended, sensory modality (touch or vision, respectively). In contrast to previous studies and to what happens with spatial attention, we found that events in one (unattended) modality do not automatically benefit from happening at the time point when another modality is expected. Instead, it seems that attention can be deployed in time with relative independence for different sensory modalities. Based on these findings, we argue that temporal orienting of attention can be cross‐modally decoupled in order to flexibly react according to the environmental demands, and that the efficiency of this selective decoupling unfolds in time.
Recovery of apparent diffusion coefficient after ischemia-induced spreading depression relates to cerebral perfusion gradient.

BACKGROUND AND PURPOSE Transient decreases of the apparent diffusion coefficient (ADC) of water as measured by fast diffusion-weighted imaging (DWI) in the ischemic border zone are thought to reflect cellular swelling associated with spreading depression. DWI and dynamic contrast-enhanced MRI were applied to study the characteristics of spreading depression and the correlation between ADC recovery time and tissue perfusion in focal ischemia. METHODS Serial DWI was performed during remote middle cerebral artery occlusion in rats (n = 5) with an echo-planar imaging technique. ADC maps were calculated and ADC values displayed as a function of time in user-defined regions of interest with a time resolution of 12 to 16 seconds. Dynamic contrast-enhanced MRI was performed for qualitative correlation of ADC changes with tissue perfusion. RESULTS Recovery time of transient ADC decreases correlated with the degree of the perfusion deficit (r = .81, P < .001). Slowly recovering ADC declines were found close to the ischemic core and correlated with severe perfusion deficit, while short-lasting ADC declines were typically found in moderately malperfused or normal tissue. Transient ADC decreases originated in the subcortical and cortical ischemic border zones and propagated along the cortex with a velocity of 2.9 +/- 0.9 mm/min. CONCLUSIONS The variation in the recovery time of transient ADC decreases in the ischemic periphery reflects the gradient of the tissue perfusion. Severely delayed recovery time after spreading depression is thought to represent the ischemic penumbra.
Identification of a human monoclonal Fab with neutralizing activity against H3N2 influenza A strain from a newly constructed human Fab library

A combinatorial Fab library was constructed in pComb3H phagemid vectors, using RNA from peripheral blood lymphocytes of a healthy volunteer who had recovered from an influenza A virus infection. The library contained approximately 1.3 × 108E. coli transformants. Bio‐panning was carried out against an influenza vaccine containing components of influenza A/New Caledonia/20/99 (H1N1), A/Panama/2007/99 (H3N2), and B/Shandong/7/97 for the enrichment of phages displaying human Fab specific to the viral proteins. E. coli transformed with IF1A11, 1 of 94 randomly selected clones, displayed a human Fab antibody molecule (FabIF1A11) with efficient neutralizing activity against H3N2 influenza A virus strains. The purified FabIF1A11 demonstrated neutralizing activity against A/Okayama/6/01 (H3N2) and A/Kitakyushu/159/93 (H3N2) with 50% plaque reduction neutralization titers of 0.11 μg/ml (2.2 nM) and 1.4 μg/ml (28 nM) respectively. However, FabIF1A11 did not show neutralizing activity against the influenza A virus strain A/USSR/77 (H1N1) or the influenza B virus strain B/Kanagawa/73, even at a concentration of 20 μg/ml (400 nM). The Kd of FabIF1A11 was calculated as 3.6 × 10−9 M. FabIF1A11 was estimated to recognize a conformational epitope on the hemagglutinin of A/Okayama/6/01 (H3N2). The human monoclonal Fab product FabIF1A11 may have potential as a therapeutic or short‐term prophylactic molecule for humans with influenza A H3N2 infection.
Cap-dependent RNA splicing in a HeLa nuclear extract.

We have studied the involvement of the 5' cap structure in the splicing of precursor mRNAs in a HeLa nuclear extract. We show that precursor mRNAs are spliced efficiently only when they possess a cap structure and that preincubation of a HeLa nuclear extract rendered the splicing reaction highly sensitive to inhibition by cap analogues. This sensitization was dependent on exogenous Mg2+ but not exogenous ATP or GTP. These results demonstrate that splicing in a nuclear extract is highly dependent on the cap structure, as was demonstrated for the splicing process in a HeLa whole-cell extract [Konarska, M. M. , Padget, R. A. & Sharp, P. A. (1984) Cell 38, 731-736], and thus support the contention that cap recognition is an important feature of eukaryotic mRNA biogenesis.
Business Network-Based Value Creation in Electronic Commerce

Information technologies (IT) have affected economic activities within and beyond the boundaries of the firm, changing the face of e-commerce. This article explores the circumstances under which value is created in business networks made possible by IT. Business networks combine the capabilities of multiple firms to produce and deliver products and services that none of them could more economically produce on its own and for which there is demand in the market. We call this business network-based value creation. We apply economic theory to explain the conditions under which business networks will exist and are able to sustain their value-producing activities. Informedness has the potential to increase the complexity of consumer demand. Addressing this demand requires flexible production and delivery of products and services, and can be achieved by value-adding business networks supported by IT, standardized technology, and business process solutions. We also examine the benefits associated with business network-based value creation and fair value-sharing to support the sustainability of business networks. We develop a set of propositions and draw upon multiple case examples from the travel and hospitality industry to validate our theoretical perspectives on business network-based value creation. The results demonstrate that this industry is going through a digital transformation that makes it possible for many firms to engage in highly effective and innovative network-based value creation.
Separating features using colored snippet graphs

In feature-oriented software product lines, two complementary approaches to implement features exist: compositional and annotative ones. To combine the respective advantages, a unified representation of the two on the file-system level is desirable, yet unavailable. We pick up this demand and propose a novel concept for separating features using colored snippet graphs instead of directories and files. In particular, our proposed ideas are based on snippets, ASTs and coloring, and enable the implementation of product lines with both compositional and annotative views on real documents. As a basis for future tools, we provide a conceptual overview of our approach, discuss our ideas for leveraging snippets, and demonstrate applicability using an example scenario.
All-Trans-Retinoic Acid Improves Cholestasis in α-Naphthylisothiocyanate–Treated Rats and Mdr2−/− Mice

Chronic cholestasis results in liver injury and eventually liver failure. Although ursodeoxycholic acid (UDCA) showed limited benefits in primary biliary cirrhosis, there is an urgent need to develop alternative therapy for chronic cholestatic disorders. Previous studies from our laboratory demonstrated that all-trans-retinoic acid (atRA) is a potent suppressor of CYP7A1, the rate-limiting enzyme in bile acid synthesis. atRA also repressed the expression of tumor growth factor-β and collagen 1A1 in activated primary human stellate cells and LX2 cells. When administered together with UDCA to bile duct–ligated rats, this combined therapy significantly reduced the bile acid pool size and improved liver conditions. To further examine whether atRA alone or in combination with UDCA has greater beneficial effects than UDCA treatment alone, we assessed this treatment in two additional chronic cholestatic rodent models: α-naphthylisothiocyanate (ANIT)–treated rats and the Mdr2−/− (Abcb4−/−) knockout mouse. atRA alone significantly reduced bile duct proliferation, inflammation, and hydroxyproline levels in ANIT-treated rats, whereas the combination of atRA and UDCA significantly reduced plasma bile salt level compared with UDCA treatment. atRA alone or in combination with UDCA significantly reduced plasma levels of alkaline phosphatase and bile salts in 12-week-old Mdr2−/− mice. Reduced bile duct proliferation and inflammation were also observed in the livers of these mice. Together, atRA alone or in combination with UDCA significantly reduced the severity of liver injury in these two animal models, further supporting the combination treatment of atRA and UDCA as a potential new therapy for patients with chronic cholestatic liver disease who have not responded fully to UDCA.
Sapphire: copying GC without stopping the world

Many concurrent garbage collection (GC) algorithms have been devised, but few have been implemented and evaluated, particularly for the Java programming language. Sapphire is an algorithm we have devised for concurrent copying GC. Sapphire stresses minimizing the amount of time any given application thread may need to block to support the collector. In particular, Sapphire is intended to work well in the presence of a large number of application threads, on small- to medium-scale shared memory multiprocessors. A specific problem that Sapphire addresses is not stopping all threads while thread stacks are adjusted to account for copied objects (in GC parlance, the “flip” to the new copies). Sapphire extends previous algorithms, and is most closely related to replicating copying collection, a GC technique in which application threads observe and update primarily the old copies of objects [13]. The key innovations of Sapphire are: (1) the ability to “flip” one thread at a time (changing the thread's view from the old copies of objects to the new copies), as opposed to needing to stop all threads and flip them at the same time; and (2) avoiding a read barrier.
An Institutional Perspective on Performance Measurement and Management in the 'New Public Sector'

In what has become known as "The New Public Sector", in the 1990s many services in advanced economics such as those of the UK and Scandinavia have come under pressure to become more efficient and effective so as to reduce their demands on taxpayers while maintaining the volume and quality of services supplied to the public. To achieve this they have been subjected to the introduction of various "private sector" management techniques and the frequent adoption of some form of neo-market system in which the purchasers and providers of public services have been split and frequently required to contract with each other. In this paper, we explore the implications of institutional theory for the successful public sector. In particular, broadening the two-party, funders and professional service providers framework of traditional institutional theory to include purchasers of public services allows us to analyse the likely impact of purchaser: provider splits on multi-dimensional performance measurement systems in the public sector. We show that the differing nature of the interrelationships between these three key stakeholders will influence the extent to which performance measurement in the focal service-provider organisations will be balanced and integrated. We also discuss the influence of these core concepts on the possibilities of achieving some balance between the stakeholders interests examined in the overall control of provider organizations. Five research propositions are advanced, three relating to the relationships between the focal organizations and funders and professional service providers, respectively, and two concerning the focal organization? s links with purchasers. Future empirical research in this are should take the form of longitudinal case studies to track differing paths of development and their effects through time.
HIV-1 Replication Is Differentially Regulated by Distinct Clinical Strains of Mycobacterium tuberculosis

Background Tuberculosis (TB) is the largest cause of death in human immunodeficiency virus type 1 (HIV-1) infection, having claimed an estimated one third to one half of the 30 million AIDS deaths that have occurred worldwide. Different strains of Mycobacterium tuberculosis (MTb), the causative agent of TB, are known to modify the host immune response in a strain-specific manner. However, a MTb strain-specific impact upon the regulation of HIV-1 replication has not previously been established. Methology/Principal Findings We isolated normal human peripheral blood mononuclear cells (PBMC) and co-infected them with HIV-1 and with either the well characterized CDC1551 or HN878 MTb clinical isolate. We show that HIV-1 co-infection with the CDC1551 MTb strain results in higher levels of virus replication relative to co-infection with the HN878 MTb strain ex vivo. Furthermore, we show that the distinct pattern of CDC1551 or HN878 induced HIV-1 replication is associated with significantly increased levels of TNF and IL-6, and of the transcription and nuclear translocation of the p65 subunit of the transcription factor NF-κB, by CDC1551 relative to HN878. Conclusions/Significance These results provide a precedent for TB strain-specific effects upon HIV-1 replication and thus for TB strain-specific pathogenesis in the outcome of HIV-1/TB co-infection. MTb strain-specific factors and mechanisms involved in the regulation of HIV-1 during co-infection will be of importance in understanding the basic pathogenesis of HIV-1/TB co-infection.
Genomewide Multiple-Loci Mapping in Experimental Crosses by Iterative Adaptive Penalized Regression

Genomewide multiple-loci mapping can be viewed as a challenging variable selection problem where the major objective is to select genetic markers related to a trait of interest. It is challenging because the number of genetic markers is large (often much larger than the sample size) and there is often strong linkage or linkage disequilibrium between markers. In this article, we developed two methods for genomewide multiple loci mapping: the Bayesian adaptive Lasso and the iterative adaptive Lasso. Compared with eight existing methods, the proposed methods have improved variable selection performance in both simulation and real data studies. The advantages of our methods come from the assignment of adaptive weights to different genetic makers and the iterative updating of these adaptive weights. The iterative adaptive Lasso is also computationally much more efficient than the commonly used marginal regression and stepwise regression methods. Although our methods are motivated by multiple-loci mapping, they are general enough to be applied to other variable selection problems.
SQL Anywhere: A Holistic Approach to Database Self-management

In this paper we present an overview of the self-management features of SQL anywhere, a full-function relational database system designed for frontline business environments with minimal administration. SQL Anywhere can serve as a high-performance workgroup server, an embedded database that is installed along with an application, or as a mobile database installed on a handheld device that provides full database services, including two-way synchronization, to applications when the device is disconnected from the corporate intranet. We illustrate how the various self-management features work in concert to provide a robust data management solution in zero-administration environments.
Examination of the Messages Preschool Teachers Use against Undesirable Behaviors of Children.

The purpose of this study was to examine, through in-class observations, the messages preschool teachers use against children's undesirable behaviors, in order to warn the children and remove negative behaviors. The study group consisted of six preschool teachers. The messages used by preschool teachers against undesirable behaviors of children were observed with the "unstructured and nonparticipant observation" technique. Notes were taken, and voice recorder was used for recording data. In the analysis of the data obtained in the study, content analysis from qualitative research methods was used. According to the findings of the study, it was determined that in the face of the undesirable behaviors of children preschool teachers most commonly use solution messages from you-messages and least commonly use I-messages. Key words: Preschool teacher, undesirable behaviors, messages used by teachers
Visualizing Social Structure: Bridging The Gap Between Contemporary Social Theory And Social Network Analysis

Social network analysis (SNA) has much to offer sociology. However, there is little consideration of SNA in contemporary sociological theory (CST) although SNA developed in parallel with the consolidation of CST in the 1960s and 1970s. SNA championed its distinctiveness as 'structural analysis'. Nearly all current CST presents 'structure versus agency' as the crucial binary to differentiate mainstream theory, positivistic ontology and quantitative methodologies, on the one hand, from interactionist theories, qualitative methodologies and constructivist ontologies on the other. I argue that using structure as a simple binary means that the CST's notions of structure have become highly ambiguous and ill defined. Thus, for CST, 'structural analysis' has no purchase to describe the distinctiveness of SNA. I draw attention to the recent work of Lopez and Lopez and Scott that reclaims varied concepts of structure from previous literatures, including a concept of 'relational structure' that embraces SNA. The paper concludes that the work of Lopez is an important example of the 'visual' method of social theory practice advocated by Woodiwiss. Once we work with 'social-theoretical picturing' of structure, there is real potential to connect CST to the resources available in the visual, statistical and mathematical modeling developed by SNA.