Miracle-dz/UniFAR-FTUs huggingface dataset:
Theta: Rapid installation and acceptance of an XC40 KNL system

In order to provide a stepping stone from the Argonne Leadership Computing Facility's (ALCF) world class production 10 petaFLOP IBM BlueGene/Q system, Mira, to its next generation 200 petaFLOPS 3rd generation Intel Xeon Phi system, Aurora, ALCF worked with Intel and Cray to acquire an 8.6 petaFLOPS 2nd generation Intel Xeon Phi–based system named Theta. Theta was delivered, installed, integrated, and accepted on an aggressive schedule in just over 3 months. We will detail how we were able to successfully meet the aggressive deadline as well as lessons learned during the process.
Effects of cooling on meiotic spindle structure and chromosome alignment within in vitro matured porcine oocytes

Meiotic spindle structure and chromosome alignment were examined after porcine oocytes were cooled at metaphase II (M II) stage. Cumulus–oocyte complexes (COCs) collected from medium size follicles were cultured in an oocyte maturation medium at 39°C, 5% CO2 in air for 44 hr. At the end of culture, oocytes were removed from cumulus cells and cooled to 24 or 4°C for 5, 30, or 120 min in a solution with or without 1.5 M dimethyl sulfoxide (DMSO). After being cooled, oocytes were either fixed immediately for examination of the meiotic spindle and chromosome alignment or returned to maturation medium at 39°C for 2 hr for examination of spindle recovery. Most oocytes (65–71%) cooled to 24°C showed partially depolymerized spindles but 81–92% of oocytes cooled at 4°C did not have a spindle after cooling for 120 min. Quicker disassembly of spindles in the oocytes was observed at 4°C than at 24°C. Cooling also induced chromosome abnormality, which was indicated by dispersed chromosomes in the cytoplasm. Limited spindle recovery was observed in the oocytes cooled to both 4 and 24°C regardless of cooling time. The effect of cooling on the spindle organization and chromosome alignment was not influenced by the presence of DMSO. These results indicate that the meiotic spindles in porcine M II oocytes are very sensitive to a drop in the temperature. Both spindle and chromosomes were damaged during cooling, and such damage was not reversible by incubating the oocytes after they had been cooled. Mol. Reprod. Dev. 65: 212–218, 2003. © 2003 Wiley‐Liss, Inc.
Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host

Specific serological diagnosis of equine herpesvirus 4 (EHV4; equine rhinopneumonitis virus) and EHV1 (equine abortion virus) hitherto has not been possible because of extensive antigenic cross-reactivity between these two closely related but distinct viruses. Recently, we identified EHV4 glycoprotein G (gG) and characterized it as a type-specific, secreted glycoprotein (B. S. Crabb, H. S. Nagesha, and M. J. Studdert, Virology 190:143-154, 1992). This paper shows that EHV1 gG also possesses type-specific epitopes and describes the localization of strong, type-specific epitopes to the apparently corresponding and highly variable regions comprising amino acids 287 to 382 of EHV4 gG and 288 to 350 of EHV1 gG. Fusion proteins expressing these variable regions reacted strongly and type specifically with sera from four foals, three of which were colostrum-deprived, specific-pathogen-free foals, whose history with respect to exposure to EHV4 or EHV1 was well-defined. These antigens provided the basis for the development of a single-well diagnostic enzyme-linked immunosorbent assay to distinguish horses infected with EHV4, EHV1, or both. Such a type-specific test provides for the first time the opportunity to differentiate antibodies to these viruses, and it has, therefore, important implications for understanding the epidemiology of these equine pathogens. Evidence for the existence of EHV1 in Australia 10 years prior to the first confirmed case of EHV1 abortion is presented.
Analisis Spasial Kejadian Tuberkulosis di Daerah Dataran Tinggi Kabupaten Gowa

Suhu yang rendah dan kelembaban udara yang tinggi di daerah dataran tinggi merupakan keadaan yang dapat mendukung perkembangbiakan bakteri Mycobacterium tuberculosis. Penelitian ini bertujuan untuk menganalisis kejadian tuberkulosis berdasarkan kondisi fisik rumah di daerah dataran tinggi Kabupaten Gowa. Jenis penelitian ini adalah penelitian kuantitatif dengan rancangan observasional deskriptif dengan menggunakan pendekatan Sistem Informasi Geografis (SIG). Jumlah populasi adalah seluruh pasien tuberkulosis yang tercatat pada rekam medik di Puskesmas Parangloe, Tinggimoncong dan Tamaona mulai bulan Januari-Desember 2016. Jumlah sampel sebanyak 57 orang. Teknik pengambilan sampel yaitu total sampling. Analisis data spasial menggunakan Quantum GIS sedangkan analisis deskriptif menggunakan SPSS 17.0. Hasil penelitian menunjukkan bahwa distribusi kejadian tuberkulosis berdasarkan lingkungan fisik rumah yang memenuhi syarat yaitu: suhu sebanyak 43 rumah (75.4%), kelembaban sebanyak 17 rumah (29.8%), luas ventilasi sebanyak 11 rumah (19.3%), kepadatan hunian sebanyak 50 rumah (87.7%), kondisi lantai sebanyak 22 rumah (38.6%), dan kondisi dinding sebanyak 21 rumah (36.8%). Overlay kondisi lingkungan fisik rumah dan kejadian tuberkulosis tertinggi 33% sebanyak 18 rumah. Buffer jarak rumah responden dengan puskesmas, jauh sebanyak 33 rumah (57.9%) dan dekat sebanyak 24 rumah (42.1%). Gradasi ketinggian wilyah tempat tinggal penderita tuberkulosis yaitu 200 m dpl sebanyak 43 rumah (75.4%). Kesimpulan dari penelitian ini adalah faktor lingkungan fisik rumah yang memenuhi syarat adalah suhu dan kepadatan hunian sedangkan yang tidak memenuhi syarat adalah kelembaban, luas ventilasi, kondisi lantai dan kondisi dinding. Sebagian besar rumah penderita tuberkulosis tidak memenuhi syarat, jauh dari pusat pelayanan kesehatan, dan berada di ketinggian >200 m dpl. Diperlukan penelitian lebih lanjut terkait analisis spasial kejadian tuberkulosis di daerah dataran tinggi dengan melihat faktor-faktor penyebab tuberkulosis lainnya.
Adsorption from saliva to silica and hydroxyapatite surfaces and elution of salivary films by SDS and delmopinol

The adsorption of proteins from human whole saliva (HWS) onto silica and hydroxyapatite surfaces (HA) was followed by quartz crystal microbalance with dissipation (QCM-D) and ellipsometry. The influence of different surface properties and adsorption media (water and PBS) on the adsorption from saliva was studied. The viscoelastic properties of the salivary films formed on the solid surfaces were estimated by the use of the Voigt-based viscoelastic film model. Furthermore, the efficiency of SDS and delmopinol to elute the adsorbed salivary film from the surfaces was investigated at different surfactant concentrations. A biphasic kinetic regime for the adsorption from saliva on the silica and HA surfaces was observed, indicating the formation of a rigidly coupled first layer corresponding to an initial adsorption of small proteins and a more loosely bound second layer. The results further showed a higher adsorption from HWS onto the HA surfaces compared to the silica surfaces in both adsorption media (PBS and water). The adsorption in PBS led to higher adsorbed amounts on both surfaces as compared to water. SDS was found to be more efficient in removing the salivary film from both surfaces than delmopinol. The salivary film was found to be less tightly bound onto the silica surfaces since more of the salivary film could be removed with both SDS and delmopinol compared to that from the HA surface. When adsorption took place from PBS the salivary layer formed at both surfaces seemed to have a similar structure, with a high energy dissipation implying that a softer salivary layer is built up in PBS as opposed to that in water. Furthermore, the salivary layers adsorbed from water solutions onto the HA were found to be softer than those on silica.
A Flexible Framework for WideSpectrum VR Development

This paper presents results and experiences coming from 10 years of development and use of XVR, a flexible, general-purpose framework for virtual reality VR development. The resulting architecture, that comes under the form of a self-sufficient integrated development environment (IDE) organized around a dedicated scripting language and a virtual machine, is able to accommodate a wide range of applications needs, ranging from simple Web3D applications to motion-based simulators or complex cluster-based immersive visualization systems. Within the framework a common, archetypical structure is used for any application, showing how inhomogeneous needs and technologies can be effectively covered by using a single, rather simple, system organization. We also show how the framework flexibility allows for innovative development techniques such as multiple frameworks coexisting within a single, tightly integrated, VR application.
Quantitative profiling of LNCaP prostate cancer cells using isotope‐coded affinity tags and mass spectrometry

Androgens are involved in the pathogenesis of diseases including adenocarcinoma of the prostate. These hormones are important for growth, maintenance, and integrity of structure and function of the prostate. Androgen‐deprivation is currently the only effective systemic therapy for prostate cancer but the effects of androgens on the proteome are still poorly described. Here we quantitatively profile changes in the proteome of LNCaP human prostate cancer cells in response to androgen using the newly developed isotope‐coded affinity tag (ICAT) labeling and two‐dimensional liquid chromatography‐tandem mass spectroscopy (2‐D LC‐MS/MS). ICAT enables the concurrent identification and comparative quantitative analysis of proteins present in various biological samples including human cell and tissue extracts. Quantification and identification of 139 proteins in complex protein mixtures obtained from androgen‐stimulated and unstimulated LNCaP cells were achieved. Changes in levels of 77 proteins in response to androgens were detected. Some of these proteins have been previously reported to be regulated by androgens and include spermine synthase, fatty acid synthase and calreticulin precursor. A large number of proteins that have not been previously reported to be expressed in prostate cells were also quantitatively identified. Examples of these include members of the dual specificity protein phosphatase subfamily, “similar” to hypothetical protein DKFZp434B0328.1, “similar” to 14‐3‐3 protein zeta and “similar” to hypothetical protein 458, components of the actin cytoskeleton and a range of unknown/uncharacterized proteins. This catalogue of proteins provides an overview of the proteome of prostate cancer cells and the global changes that occur in response to androgens.
Uncovered Interest Parity and Monetary Policy Freedom in Countries with the Highest Degree of Financial Openness

This study reviews the uncovered interest parity hypothesis in perfectly open economies. To meet the assumption of perfect capital mobility, we only include countries and periods with the highest degree of capital account openness in the sample. Using several specifications from the restricted to the less restricted models, we estimate the relationship among exchange rate, domestic and benchmark interest rates. Regardless of the exchange rate regime, we find evidence that domestic interest rate is highly sensitive to international interest rate. Therefore, monetary policy independence would be affected.
Involvement of the NMDA receptor, NO‐cyclic GMP and nuclear factor K‐β in an animal model of repeated trauma

Post‐traumatic stress disorder (PTSD) may be associated with shrinkage of the hippocampus, with glutamate release causally related to these events. Recent animal studies strongly implicate activation of the nitric oxide (NO)‐cascade in anxiety and stress. Using an animal model of repeated trauma, the effect of stress was investigated on the hippocampal NO‐cGMP signalling pathway, specifically the release of nitrogen oxides (NOx) and its modulation by NMDA receptor‐, NO‐, cGMP‐ and nuclear factor K‐β (NFK‐β)‐selective drugs. Immediately after stress, rats received the glutamate NMDA receptor antagonist, memantine (MEM; 5 mg/kg i.p./d), the NO synthase inhibitor, 7‐nitroindazole sodium salt (7‐NINA; 20 mg/kg i.p./d), the cGMP‐specific PDE inhibitor, sildenafil (SIL; 10 mg/kg i.p./d) or the NFκ‐β antagonist, pyrollidine dithiocarbamate (PDTC; 70 mg/kg i.p./d), for 7 days. Stress significantly increased hippocampal NOx on day 7 post‐stress, which was blocked by either 7‐NINA or PDTC, while MEM was without effect. SIL, however, significantly augmented stress‐induced NOx accumulation. Increased cGMP therefore acts as a protagonist in driving stress‐related events, while both nNOS (neuronal NOS) and iNOS (inducible/immunological NOS) may represent a therapeutic target in preventing the effects of severe stress. The value of NMDA receptor antagonism, however, appears limited in this model. Copyright © 2005 John Wiley & Sons, Ltd.
Ultrastructural changes in Raillietina (Platyhelminthes: cestoda), exposed to sulfonoquinovosyldiacylglyceride (SQDG), isolated from Neem (Azadirachta indica)

Abstract Neem (Azadirachta indica), has been known to be a curative for various ailments and diseases in the traditional Indian medicinal system from times immemorial. A glycolipid sulfonoquinovosyldiacylglyceride (SQDG) isolated from the leaves of neem has been found to be a proactive antibacterial and antiviral agent in previous studies. The current communication pertains to the anthelmintic activity of SQDG in vitro against a model cestode Raillietina spp. The results of efficacy tests showed a paralysis time of 1.0 ± 0.1 and 0.7 ± 0.01 h, whereas death time of 1.6 ± 0.3 and 0.9 ± 0.02 h, following treatments with dosages of 0.5 and 1.0 mg/mL, respectively. The scanning electron microscopic studies showed significant and unique changes in the ultrastructure of the worms with prominent breakages and furrows on the surface.
Aneusomy of chromosomes 7, 8, and 17 and amplification of HER-2/neu and epidermal growth factor receptor in Gleason score 7 prostate carcinoma: a differential fluorescent in situ hybridization study of Gleason pattern 3 and 4 using tissue microarray.

Recent evidence shows that the proportion of poorly differentiated prostate carcinoma (Gleason pattern [GP] 4/5) is a surrogate factor for biochemical failure after radical prostatectomy (RP). However, little is known about specific molecular and cytogenetic changes in this aggressive component of localized prostate cancer. We constructed a tissue microarray containing areas of GP 3 and 4 from formalin-fixed radical prostatectomy specimens of 39 patients with Gleason score 7 carcinoma (>or=50% GP 4), known pathologic staging parameters (stage < T3b), and biochemical failure data (mean follow-up, 30 months; range, 5 to 74 months). Interphase fluorescent in situ hybridization (FISH) was performed on 5-microm microarray sections using pericentromeric probes to chromosomes 7, 8, and 17 and probes for the HER-2/neu and epidermal growth factor receptor (EGFR) genes. Low-level amplification of HER-2/neu was found in 26% of cases (3 to 5 signals per nucleus, corrected for chromosome 17 aneusomy). Aneusomy of chromosomes 7, 8, and 17 was identified in 21%, 15%, and 5% of cases, respectively. All aberrations occurred almost exclusively in GP 4 carcinoma (8 of 8 aneusomies 7, 2 of 2 trisomies 17, 9 of 10 HER-2/neu amplifications, and 5 of 6 aneusomies 8; P < .001). The presence of HER-2/neu amplification was associated with high tumor volume (>2.0 cm(3), P = 0.004). Among patients with negative surgical margins, gain of chromosome 7 was associated with biochemical failure after RP (P =.004, log-rank). Amplification of the EGFR gene occurred in only 1 case (3%). Significant differences in HER-2/neu amplification and gain of chromosomes 7, 8, and 17 were detected between GP 4 prostate carcinoma and GP 3. The frequency of aberrations increased with tumor volume. Chromosome 7 abnormalities may play an important role in cancer progression in margin-negative patients. EGFR amplification was rare, suggesting that this oncogene is not altered at the gene copy number level.
Ca2+ Regulation of Interactions between Endoplasmic Reticulum Chaperones*

Casade Blue (CB), a fluorescent dye, was used to investigate the dynamics of interactions between endoplasmic reticulum (ER) lumenal chaperones including calreticulin, protein disulfide isomerase (PDI), and ERp57. PDI and ERp57 were labeled with CB, and subsequently, we show that the fluorescence intensity of the CB-conjugated proteins changes upon exposure to microenvironments of a different polarity. CD analysis of the purified proteins revealed that changes in the fluorescence intensity of CB-ERp57 and CB-PDI correspond to conformational changes in the proteins. Using this technique we demonstrate that PDI interacts with calreticulin at low Ca2+ concentration (below 100 μm), whereas the protein complex dissociates at >400 μmCa2+. These are the Ca2+ concentrations reminiscent of Ca2+ levels found in empty or full ER Ca2+ stores. The N-domain of calreticulin interacts with PDI, but Ca2+ binding to the C-domain of the protein is responsible for Ca2+ sensitivity of the interaction. ERp57 also interacts with calreticulin through the N-domain of the protein. Initial interaction between these proteins is Ca2+-independent, but it is modulated by Ca2+binding to the C-domain of calreticulin. We conclude that changes in ER lumenal Ca2+ concentration may be responsible for the regulation of protein-protein interactions. Calreticulin may play a role of Ca2+ “sensor” for ER chaperones via regulation of Ca2+-dependent formation and maintenance of structural and functional complexes between different proteins involved in a variety of steps during protein synthesis, folding, and post-translational modification.
The Social Environment and Medicaid Expenditures: Factors Influencing the Level of State Medicaid Spending

including a callfor greater state-level management. The state Medicaid programs, financed jointly with federal and state funds, were created in 1965 to provide health coverage to the poor. One of the largest domestic programs, combined federal and state expenditures for Medicaid coverage of health care services are projected to exceed $66 billion during 1991.1 During fiscal year 1988, Medicaid expenditures approached $49 billion while covering the health services of 22.9 million beneficiaries.2
The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene

The product of the rpoN gene is an alternative sigma factor of RNA polymerase which is required for transcription of a number of genes in members of the family Enterobacteriaceae, including those that specify enzymes of nitrogen assimilation, amino acid uptake, and degradation of a variety of organic molecules. We have previously shown that transcription of the pilin gene of Pseudomonas aeruginosa also requires RpoN (K. S. Ishimoto and S. Lory, Proc. Natl. Acad. Sci. USA 86:1954-1957, 1989) and have undertaken a more extensive survey of genes under RpoN control. Strains of P. aeruginosa that carry an insertionally inactivated rpoN gene were constructed and shown to be nonmotile because of the inability of these mutants to synthesize flagellin. The mutation in rpoN had no effect on expression of extracellular polypeptides, outer membrane proteins, and the alginate capsule. However, the rpoN mutants were glutamine auxotrophs and were defective in glutamine synthetase, indicating defects in nitrogen assimilation. In addition, the P. aeruginosa rpoN mutants were defective in urease activity. These findings indicate that the sigma factor encoded by the rpoN gene is used by P. aeruginosa for transcription of a diverse set of genes that specify biosynthetic enzymes, degradative enzymes, and surface components. These rpoN-controlled genes include pili and flagella which are required for full virulence of the organism.
Stress reduces the incorporation of misinformation into an established memory

Memory can be distorted by misleading post-event information. These memory distortions may have serious consequences, for example in eyewitness testimony. Many situations in which memory reports are solicited, and suggestive or misleading information is presented, are highly stressful for the respondent, yet little is known about how stress affects people's susceptibility to misinformation. Here, we exposed participants to a stressor or a control manipulation before they were presented misinformation about a previous event. We report that stressed participants endorsed misinformation in a subsequent memory test less often than control participants, suggesting that stress reduces distortions of memory by misleading information.
A study on creative elements detection in application domain workflow based on educational psychology theories

Creativity is considered as a very important element of the society development. Having entered the big data era, people have been focusing on finding a pathway developing creativity for all applications. In the psychology and education domain, various approaches have been attempted, such as divergent thinking and brain storming. The psychology domain has researched on human's cognitive development and influential elements. However, existing approaches are unable to present a specific guidance for an individual to develop creativity. This research aims at apply psychology, education and creative computing research outcomes to seek for a reliable workflow to generate a feasible education scheme through detecting creative elements from the history of a target application domain. In this way, people can be clear for both negative and positive elements. The workflow also implements computing algorithms to make the data processing more accurate and scientific. The whole workflow includes three stages: searching for the history, selecting theorists' classification, and results matching and integrating. The final outcome will be an education scheme containing both positive and negative elements for the user.
Mobile Agents for Location-Aware Advertising in Mobile Wireless Environments

Advertisements surround our daily lives and are becoming increasingly ubiquitous. However, unless the advertisements are highly relevant to the consumer, she is likely not to pay full attention to it. In this paper, we propose a novel mobile agentbased system where businesses can better understand and communicate with the consumer. Meanwhile, this system also helps the end user reduce information overload by helping her filter out unnecessary information and only show information that is relevant. The proposed system is a marked departure from the traditional client-server architecture commonly found in current e-commerce systems. It provides novel ways for cellular service providers to compete, and businesses to acquire new customers. In this paper, we will address all the challenges associated with such an evolutionary system. We will also provide some sample scenarios, along with an architectural design for our proposed system.