Miracle-dz/UniFAR-FTUs huggingface dataset:
The Glucose-regulated Nuclear Localization of Hexokinase 2 in Saccharomyces cerevisiae Is Mig1-dependent*

Two major mediators of glucose repression in Saccharomyces cerevisiae are the proteins Mig1 and Hxk2. The mechanism of Hxk2-dependent glucose repression pathway is not well understood, but the Mig1-dependent part of the pathway has been elucidated in great detail. Here we report that Hxk2 has a glucose-regulated nuclear localization and that Mig1, a transcriptional repressor responsible for glucose repression of many genes, is required to sequester Hxk2 into the nucleus. Mig1 and Hxk2 interacted in vivo in a yeast two-hybrid assay and in vitro in immunoprecipitation and glutathione S-transferase pull-down experiments. We found that the Lys6-Met15 decapeptide of Hxk2, which is necessary for nuclear localization of the protein, is also essential for interaction with the Mig1 protein. Our results also show that the Hxk2-Mig1 interaction is of physiological significance because both proteins have been found interacting together in a cluster with DNA fragments containing the MIG1 site of SUC2 promoter. We conclude that Hxk2 operates by interacting with Mig1 to generate a repressor complex located in the nucleus of S. cerevisiae during growth in glucose medium.
Cloud‐based healthcare management: Identifying the privacy concerns and their moderating effect on the cloud‐based health‐care services

This article studies about cloud healthcare management, where the cloud computing services seems as one of the viable options to improve the health‐care services but simultaneously faces certain deterrents in the form of privacy concerns of the service users. This study aims to identify the privacy concerns, followed by analyzing the moderating effect of these concerns over the success parameters which favors the successful adoption of cloud‐based healthcare services. A total of 20 success parameters and nine privacy concerns of cloud‐based healthcare service users have been identified by doing a thorough literature review. The success parameters are the independent variables, privacy concerns are the moderating variables, and the factors defining success measures are the dependent variables. These three types of variables have been constituted to propose three models which identify the direct as well as the moderating effect of these privacy concerns over the critical success factors (CSFs) for the successful adoption of cloud‐based healthcare services. These have been factually tested by analysis done on data collected from 105 respondents of the related domain. Structural equation modeling has been used to determine the moderating effect of the privacy concerns over the success factors. The latent variables used in the models successfully pass discriminant validity, composite reliability, multicollinearity, indicator correlation, and factor analysis test. The observed outcome demonstrates that privacy concerns of cloud‐based healthcare service users are moderating the organizational and people‐related success factors, whereas it does not moderate the technology‐related success factors for successful adoption of cloud‐based healthcare services. The privacy concerns of such service users and the CSFs (ie, organizational‐, people‐, technology‐related success factors) directly and significantly affect the adoption rate of cloud‐based healthcare services. Thus the article produces a data‐based proof and statistically analyzed basis for the companies and healthcare organizations involved in the development and maintenance of cloud‐based healthcare services by categorically breaking down the effect of success parameters of cloud adoption along with pointing out the concerns to be addressed to convincingly satisfy the service users.
Essential and selective role of SNX12 in transport of endocytic and retrograde cargo

ABSTRACT The endosomal protein-sorting machineries play vital roles in diverse physiologically important cellular processes. Much of the core membrane-sorting apparatus is conserved in evolution, such as retromer, which is involved in the recycling of a diverse set of cargoes via the retrograde trafficking route. Here, in an RNAi-based loss-of-function study, we identified that suppression of SNX12 leads to a severe blockage in CIM6PR (also known as IGF2R) transport and alters the morphology of the endocytic compartments. We demonstrate that SNX12 is involved in the early phase of CIM6PR transport, and mediates receptor recycling upstream of the other well-established SNX components of retromer. Ultra-structural analysis revealed that SNX12 resides on tubulo-vesicular structures, despite it lacking a BAR domain. Furthermore, we illustrate that SNX12 plays a key role in intraluminal vesicle formation and in the maturation of a subpopulation of early endosomes into late endosomes, thereby regulating selective endocytic transport of cargo for degradation. This study therefore provides evidence for the existence of early endosomal subpopulations that have differential roles in the sorting of the cargoes along endocytic degradative pathways. Summary: SNX12 acts as a bridge between the endocytic and retrograde pathways. There are different early endosomal subpopulations that play a differential role in sorting of the degradative cargoes.
Bad Bets: Excessive Risk-Taking, Convex Incentives, and Performance

Managerial incentives influence risk-taking as well as effort. Theoretical research has long considered risk-taking to be a potential side effect of incentive pay, but empirical analysis of risk-taking incentives has been more limited. This paper uses exogenous variation in incentives to examine how convex incentive schemes simultaneously influence performance and risk-taking. We study these questions in the context of the hedge fund industry in which the use of convex incentives is replete, given that most managers earn a combination of a base and an incentive fee if their performance exceeds a threshold. Consistent with other results in the literature, the paper first establishes that when managers fall below the threshold, above which they earn performance fees, risk-taking increases and performance drops. On average, risk-taking increases 50% and performance falls 2.3 percentage points when a hedge fund is below the incentive threshold.Having established these baseline results, the paper proceeds to more carefully examine the link between performance and risk-taking explicitly. First, given the empirical setting, we are able to separately identify the effort and risk-taking effects of being below the incentive threshold, and show that much of performance declines are due to excessive risk-taking rather than to reduced effort. Second, we show that risk-taking behavior is non-monotonic; managers who are significantly below the threshold reduce risk-taking relative to those who are relatively close. The importance of risk-taking to performance adds to the debate about the impact of incentives on behavior. Regardless of whether incentives are given or justified by concerns of moral hazard with respect to effort or risk-taking, or concerns of adverse selection or whether risk-aversion is an important consideration, risk-taking can have significant impacts. These results suggest that risk-taking, due to convex incentives, is not only inefficient-in the sense that risk-taking choices are dominated and lead to absolute performance declines.
Sublethal Effects of Spinosad on Some Biochemical Parameters of Xanthogaleruca luteola (Müller) (Coleoptera: Chrysomelidae)

Mohammadzadeh Tamam B. , Ghadamyari M. , Sahragard A. , Karimi-Malati A. ( 2014): Sublethal effects of spinosad on some biochemical parameters of Xanthogaleruca luteola (Muller) (Coleoptera: Chrysomelidae). Plant Protect. Sci., 50: 199–205. The effect of spinosad on some biochemical characteristics of Xanthogaleruca luteola was investigated. LC 30 and LC 50 of spinosad for the third instar larvae were determined as 1.6 and 2.9 ppm, respectively. Biochemical tests were carried out on the 3 rd instar larvae treated with LC 30 and LC 50 of spinosad. The LC 30 concentration decreased carbohydrate and glycogen content and increased lipid content significantly. There was a significant decrease in carbohydrate, gly cogen, and protein contents following the exposure to LC 50 concentration. Available energy of the larvae exposed to LC 30 of spinosad increased significantly, whereas it decreased significantly at LC 50 . However, the energy consumed was increased at both concentrations significantly. Cellular energy allocation of larvae treated with LC 50 concentrations of spinosad decreased significantly. Glutathione- S-transferase and esterase activities were increased significantly at LC 50 . However, lipase activity was decreased at both concentrations.
The Effect of Rural Road Transport on Crop Commercialization: Evidence from the Ethiopian Living Standard Measurement Survey

Ethiopia has adopted commercialization of smallholder agriculture as a policy tool for agricultural development and rural transformation thereby to improve rural welfare. Towards this end, the government has given policy focus to rual road infrastracture. The objective of the study is, therefore, to estimate the effect of acess to rual road on commercialization of smallholder farmers in the country. The study used the Ethiopian Socio- Economic Survey, a nationally representative panel data prepared by Central Statistics Agency of Ethiopia and the World Bank. Descriptive statistics as well as Econometric techniques are used to analyze the effect of rural accessibility and mobility on agricultural market participation and commercialization. The descriptive statistics reveals that the commercialization index for households in villages with access to all weather roads is 19 percent against the corresponding figure of 16 percent for their counterparts. The econometrics estimation also tallied with this finding. More interestingly, mode of transport used for agricultural purposes is found to have a positive and significant effect on commercialization. The result indicates that commercialization level for farmers using modern mode of transport is 17 percentage points higher than those who did not use any. In the same manner, the result indicates that commercialization level of farmers using traditional mode of transport is 12 percentage points higher than those who did not use any. Thus, integrating remote areas with urban centers through rural transport infrastructure development that addresses both access and mobility demand of rural communities should be given priority. Keywords : commercialization, market participation, rural road access, mobility
Dissecting the Effectiveness of Firm Financial Strength in Predicting Chinese Stock Market

This paper studies whether the financial strength measure, F-score, can predict returns in the Chinese stock market and its economic explanations. The results suggest that high F-score firms can generate high expected stock returns. Additionally, the predictability of F-score is robust after controlling for common factors in Fama–French models, and other firm characteristics and risks. We find that the premium generated by F-score in Chinese stock market is stronger following higher level of investor sentiment and for firms with higher limits to arbitrage and lower investment frictions, consistent with both the behavioral mispricing and the investment-q asset pricing theories.
Recombine supplier-side search via innovation ambidexterity

Purpose The purpose of this paper is to investigate the individual effects of boundary-spanning search from suppliers (supplier-side search (SS)). It is proposed that SS contributes to innovation ambidexterity (IA) and then business performance (BP). Further, this paper includes buyer–supplier relationships (BSRs) and competitive intensity (CI) as moderators to clarify boundary conditions. Design/methodology/approach An ordinary least squares regression was employed to test hypotheses, based on 184 sets of data from Hong Kong manufacturing firms. The SPSS version of PROCESS was utilized. Findings The results show that IA partially mediates the relationship between SS and BP. Contingently, the direct effect is negatively moderated by BSRs and CI. Research limitations/implications This paper confirms the partial mediating effect of IA on the relationship between SS and BP. Additional mediators, such as organizational innovation and marketing innovation, can be examined in the future. Practical implications This paper contributes to practice by suggesting that suppliers are a rewarding single source for firms to undertake boundary-spanning search. IA plays a significant role in reinforcing the effects of SS on BP and should be implemented with sustaining efforts. BSRs and CI can be detrimental and should be responded to cautiously. Originality/value This paper highlights the individual effects of SS on BP. Furthermore, the underlying process and boundary conditions are analyzed. The exploitation-exploration framework is applied throughout the entire study.
Arabidopsis Mutants with Increased Sensitivity to Aluminum

Al-sensitive (als) mutants of Arabidopsis were isolated and characterized with the aim of defining mechanisms of Al toxicity and resistance. Most als mutants selected on the basis of root growth sensitivity to Al were recessive, and together the mutants constituted eight complementation groups. Also, in most als mutants, Al sensitivity appeared to be specific for Al relative to La (another trivalent cation), except als2, which was more sensitive to La than wild type. The tendency of roots on mutant seedlings to accumulate Al was examined by staining with morin and hematoxylin, dyes used to indicate the presence of Al. A significant increase in morin staining was observed in als5, consistent with its increased sensitivity to Al. Unexpectedly, als7 and als4 showed less morin staining, suggesting that the roots on these mutants accumulate less Al than wild type after exposure to Al-containing solutions. Roots of wild-type seedlings produce callose in response to AlCl3 concentrations that inhibit root growth. Only als5 accumulated more callose than wild type in response to low levels (25 [mu]M) of AlCl3. However, als4 and als7 did not accumulate callose at this AlCl3 concentration even though root growth was significantly inhibited. The lack of callose accumulation in als4 and als7 suggests that there is not an obligatory relationship between callose deposition and Al-induced inhibition of root growth.
Trends of body composition among adolescents according to maturation stage and body mass index

Abstract Background: Excessive increases in percentage body fat (%BF) according to sex may pose greater risks of chronic diseases. Objective: To evaluate trends of body composition according to sexual maturation stage and body mass index (BMI) of adolescents. Method: Data about weight, height, lean mass, fat mass, %BF, BMI and sexual maturation of 1345 private school students (685 girls) aged from 7 to 18 years were collected in 2001 and compared with data of 1450 students (750 girls) collected in 2010 in Campinas, Brazil. χ2-test, ANOVA and logistic regression were used for statistical analyses (p<0.05). Results: Obesity prevalence increased, and number of boys and girls with normal BMI (p=0.0183 and p=0.0041) decreased. Differences in the median values of the study variables according to time point and nutritional diagnosis were not significant, except for lean mass (p=0.0484) and height (p=0.0275) in B2 (Breast stage) and %BF (p=0.0324) in G5 (Genitalia stage). Conclusion: Body composition did not change significantly, although overweight increased.
Tomography in frequency domain using wave equation

Computed tomography technology has been widely applied in many fields such as geographical structure detection, industrial detection and clinical diagnosis. In some situations the image quality is not high due to the measurement errors and disturbances. In this paper, a topographic method is studied using an acoustic wave equation to describe the propagation of the wave. Both forward modelling and image processing are performed in frequency domain to reach higher accuracy. The images are reconstructed using not only travel time but also the signal waveform to reduce errors and noise. The method has the potential of being used in clinical diagnostic ultrasound imaging.
A Rapid, Fully Automated, Molecular-Based Assay Accurately Analyzes Sentinel Lymph Nodes for the Presence of Metastatic Breast Cancer

Objective:To develop a fully automated, rapid, molecular-based assay that accurately and objectively evaluates sentinel lymph nodes (SLN) from breast cancer patients. Summary Background Data:Intraoperative analysis for the presence of metastatic cancer in SLNs from breast cancer patients lacks sensitivity. Even with immunohistochemical staining (IHC) and time-consuming review, alarming discordance in the interpretation of SLN has been observed. Methods:A total of 43 potential markers were evaluated for the ability to accurately characterize lymph node specimens from breast cancer patients as compared with complete histologic analysis including IHC. Selected markers then underwent external validation on 90 independent SLN specimens using rapid, multiplex quantitative reverse transcription-polymerase chain reaction (QRT-PCR) assays. Finally, 18 SLNs were analyzed using a completely automated RNA isolation, reverse transcription, and quantitative PCR instrument (GeneXpert). Results:Following analysis of potential markers, promising markers were evaluated to establish relative level of expression cutoff values that maximized classification accuracy. A validation set of 90 SLNs from breast cancer patients was prospectively characterized using 4 markers individually or in combinations, and the results compared with histologic analysis. A 2-marker assay was found to be 97.8% accurate (94% sensitive, 100% specific) compared with histologic analysis. The fully automated GeneXpert instrument produced comparable and reproducible results in less than 35 minutes. Conclusions:A rapid, fully automated QRT-PCR assay definitively characterizes breast cancer SLN with accuracy equal to conventional pathology. This approach is superior to intraoperative SLN analysis and can provide standardized, objective results to assist in pathologic diagnosis.
The Relationship Between the Social Environment Within Religious Organizations and Intake of Fat Versus Fruits and Vegetables

The authors explored associations of social environment with dietary behavior among participants in the Eating for a Healthy Life study, a randomized, low-fat, high-fruit-and-vegetable dietary intervention trial in religious organizations. Data in this report are from baseline telephone surveys of 1,520 persons that assessed dietary behaviors (Fat- and Fiber-Related Diet Behavior Questionnaire) and social environment (Moos Group Environment Scale). After adjusting for demographic characteristics, higher scores on the Cohesion and Order/Organization subscales were associated with higher fruit/vegetable scores (indicating higher fruit and vegetable consumption). Higher scores on the Cohesion, Leader Support, and Order/Organization subscales were also associated with lower fat scores (indicating lower fat intake). Dietary behaviors within religious organizations may be related to positive perceptions of the social environment. These results support further exploration of the potential influence of religious organizations' social environment on health behaviors and its applicability to dietary change interventions.
The influence of social capital towards the quality of community tourism services in Lake Toba Parapat North Sumatera

The objective of the research was to analyze the influence of social capital towards the quality of community tourism service In Lake Toba Parapat North Sumatera. The method used the combination between quantitative and qualitative research. Sample was taken from the Community in the area around Lake Toba Parapat North Sumatera with sample of 150 head of the family. The sampling technique was Simple Random Sampling. Data collection techniques used documentary studies, questionnaires, interview and observations, while the data analysis used were Product Moment and Simple Linear Regression analysis. The results of the research showed that there were positive and significant influence between Social Capital and the Quality of Community Tourism Services in Lake Toba Parapat North Sumatera. This research recommend the need to enhance Social Capital such as trust, norms and network and the quality of community tourism services such as Tangibles, Reliability, Responsiveness, Assurance, and Empathy by giving communications, information and education continuously from the families, institutions formal and informal, community leaders, religious figures and all communities in Lake Toba Parapat North Sumatera.
Reprogramming of Single-Cell–Derived Mesenchymal Stem Cells Into Hair Cell-Like Cells

Hypothesis Adult mesenchymal stem cells (MSCs) can be converted into hair cell-like cells by transdetermination. Background Given the fundamental role sensory hair cells play in sound detection and the irreversibility of their loss in mammals, much research has focused on developing methods to generate new hair cells as a means of treating permanent hearing loss. Although MSCs can differentiate into multiple cell lineages, no efficient means of reprogramming them into sensory hair cells exists. Earlier work has shown that the transcription factor Atoh1 is necessary for early development of hair cells, but it is not clear whether Atoh1 can be used to convert MSCs into hair cells. Methods Clonal MSC cell lines were established and reprogrammed into hair cell-like cells by a combination of protein transfer, adenoviral based gene transfer, and co-culture with neurons. During transdetermination, inner ear molecular markers were analyzed using reverse transcriptase–polymerase chain reaction, and cell structures were examined using immunocytochemistry. Results Atoh1 overexpression in MSCs failed to convert MSCs into hair cell-like cells, suggesting that the ability of Atoh1 to induce hair cell differentiation is context dependent. Because Atoh1 overexpression successfully transforms VOT-E36 cells into hair cell-like cells, we modified the cell context of MSCs by performing a total protein transfer from VOT-E36 cells before overexpressing Atoh1. The modified MSCs were transformed into hair cell-like cells and attracted contacts from spiral ganglion neurons in a co-culture model. Conclusion We established a new procedure, consisting of VOT-E36 protein transfer, Atoh1 overexpression, and co-culture with spiral ganglion neurons, which can transform MSCs into hair cell-like cells.
Multigrid Method for the Numerical Solution of the Modified Equal Width Wave Equation

Numerical solutions of the modified equal width wave equation are obtained by using the multigrid method and finite difference method. The motion of a single solitary wave, interaction of two solitary waves and development of the Maxwellian initial condition into solitary waves are studied using the proposed method. The numerical solutions are compared with the known analytical solutions. Using error norms and conservative properties of mass, momentum and energy, accuracy and efficiency of the mentioned method will be established through comparison with other methods.