Miracle-dz/UniFAR-FTUs huggingface dataset:
Objectively assessed physical activity and weight status in Maltese 11–12 year-olds

Abstract The objectives of the study were to identify levels of physical activity and sedentary time and assess how they differ by weight status in Maltese boys and girls. Participants were 234 Maltese children aged 11–12 years, of which 187 (80%) provided complete data. Physical activity was assessed using accelerometry and weight status determined through gender-specific age-adjusted Body mass index (BMI). Self-reported mode of transport to school, TV and computer time, gaming and mobile phone use were assessed by questionnaire. Total physical activity was generally very low and significantly lower for girls than boys at all times on weekdays and on weekends till 7 pm. Overweight and obesity prevalence was 27% and 18.6%, respectively. Differences in overall physical activity were observed between non-overweight, and overweight and obese boys (p=0.003). Differences in moderate to vigorous intensity physical activity (MVPA) in boys were significant across all weight categories (p=0.001) and in girls (p=0.020) between the overweight (27.6 min), the non-overweight (26.4 min) and the obese (18.9 min). For weekdays, mean physical activity differences (p=0.013) were observed between non-overweight (515.5 cpm) and obese boys (416.4 cpm). Differences in MVPA were found (p=0.038) between non-overweight (4.4 min) and obese boys in the 6 am–3 pm period. Differences were also found between overweight (13.2 min) and obese girls (8.1 min) (p=0.024) in that period. On weekends, mean physical activity differences were found between non-overweight and overweight boys from 8 am—7 pm but not for girls. Physical activity levels in this sample were very low when compared to recommended levels, while the prevalence of overweight and obesity and sedentary time were high. Girls are significantly less active than boys throughout the week. Activity level differences and patterning across the day and week were related to weight status with obese children showing markedly less activity. These results suggest that we need to provide more opportunities for overweight and obese children to be active throughout the week, with a particular emphasis on physical activity during school hours.
Comparative analysis of colorimetric staining in skin using open‐source software

Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open‐source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation‐induced tanning response of Yucatan mini‐pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples.
On Statistical Tests for Homogeneity of Two Bivariate Zero-Inflated Poisson Populations

The problem of testing treatment difference in the occurrence of a study endpoint in a randomized parallel-group comparative clinical trial with repeated responses under the assumption that the responses follow a bivariate zero-inflated Poisson (ZIP) distribution is considered. Likelihood ratio test for homogeneity of two bivariate ZIP populations is derived. Approximate formula for sample size calculation is also obtained, which achieves a desired power for detecting a clinically meaningful difference under an alternative hypothesis. An example concerning the comparison of treatment effect in an addictive clinical trial in terms of the number of days of illicit drug use during a month is given for illustrative purposes.
Isoelectric focusing in long immobilized pH gradient gels to improve protein separation in proteomic analysis

The number of protein spots detected on two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) gels increases as the gel size increases. The largest commercially available systems resolve a few thousand spots, being only a fraction of the total proteome. We have developed an extremely long isoelectric focusing (IEF) system aimed at more complete protein profiling. The system is especially well suited to sensitive detection methods, such as radioactive detection. The major constraint preventing progress in this area has been the inability to create an even density gradient during the immobilized pH gradient (IPG) casting process. We demonstrate for the first time that this constraint can be effectively overcome, to enable greatly increased IEF separating power with all the advantages of IPG technology,
Vascular Endothelial Growth Factor Receptor Tyrosine Kinase Inhibitors Vandetanib (ZD6474) and AZD2171 in Lung Cancer

Vascular endothelial growth factor (VEGF) is a rational target for advanced non–small cell lung cancer (NSCLC), a hypothesis validated by the recent Eastern Cooperative Oncology Group E4599 trial showing that the addition of the VEGF monoclonal antibody bevacizumab to chemotherapy prolongs overall survival. Several new tyrosine kinase inhibitors targeting the VEGF pathway are currently in advanced clinical development for NSCLC and offer several possible advantages compared with monoclonal antibodies, including oral administration, more flexible dosing, a broader spectrum of target inhibition, and different toxicity profiles. Among these agents, vandetanib (ZD6474), an inhibitor of the VEGF receptor (VEGFR)-2 and epidermal growth factor receptor tyrosine kinase, has been the most extensively studied. In a randomized phase II study of patients with platinum-refractory NSCLC, including squamous histology, vandetanib prolonged progression-free survival compared with gefitinib. In another phase II trial, an improvement in progression-free survival was observed for vandetanib in combination with docetaxel compared with docetaxel alone. AZD2171 is an inhibitor of VEGFR-1, VEGFR-2, and VEGFR-3 and other tyrosine kinases that has shown clinical activity in NSCLC in combination with carboplatin and paclitaxel. Several phase III trials are under way testing these agents either as monotherapy or in combination with chemotherapy in patients with lung cancer. Early results with these agents, and others being tested, raise the possibility that there will eventually be multiple VEGF-targeted therapies available in the clinic that can potentially benefit a broader range of patients with advanced-stage NSCLC.
Inclusive Design-Theory: How to take advantage of diversity in Information Systems Design

The theme of ICIS 2013 in Milan is “Reshaping Society through Information Systems ” (http://icis2013.aisnet.org/). One aspect of reshaping society that has been recently discussed in central Europe is that of social inclusion (http://ec.europa. eu/social/main.jsp? catId=750l for instance, if design principles can be formalized in order to reshape the information systems design into a different, more-inclusive direction. We contend that IS falls short in tackling this issue. In this panel, Shirley Gregor, Fred Niederman, Eileen Trauth, and Cathy Urquhart reflect on the multiple aspects of social inclusion in the design and the resulting shape of Information Systems. This panel intends to deliver more in-depth results than merely advocating a stance for more diversity in the IS workforce. Building on the principles of Design Science, we believe that our discipline can help reshape the digital economy. As a key takeaway, the panel provides guidance on the impact of gender in IS theorizing as a demonstration example, and reflect on the trend towards Social Design in the IS research community.
The Relationship between Clinico-Biochemical Characteristics and Psychiatric Distress in Young Women with Polycystic Ovary Syndrome

The relationship between clinico-biochemical characteristics and self-reported psychological parameters in 42 women with polycystic ovary syndrome (PCOS) and 42 age-matched healthy controls was examined. The General Health Questionnaire was used (GHQ-12) to ascertain emotional distress and the Beck Depression Inventory (BDI) to determine depressive symptoms. Emotional distress, depressive symptoms, hirsutism score, body mass index (BMI), waist-to-hip ratio (WHR), luteinizing hormone/follicle-stimulating hormone ratio, serum total testosterone, dehydroepiandrosterone sulphate levels and the insulin resistance index were significantly greater in women with PCOS than in healthy women. The BDI and GHQ-12 scores of the women with PCOS were significantly higher than those of the control group (BDI, 11.69 ± 9.49 vs 5.80 ± 4.58; GHQ-12, 3.38 ± 3.38 vs 1.54 ± 1.97, respectively), and BMI and WHR were positively correlated with the BDI and GHQ-12 scores. Clinicians should be aware of the increased risk of emotional distress and depression in women with PCOS, especially those who are obese, and of the need to screen these patients for such symptoms.
A Novel Adaptive Probabilistic Nonlinear Denoising Approach for Enhancing PET Data Sinogram

We propose filtering the PET sinograms with a constraint curvature motion diffusion. The edge-stopping function is computed in terms of edge probability under the assumption of contamination by Poisson noise. We show that the Chi-square is the appropriate prior for finding the edge probability in the sinogram noise-free gradient. Since the sinogram noise is uncorrelated and follows a Poisson distribution, we then propose an adaptive probabilistic diffusivity function where the edge probability is computed at each pixel. The filter is applied on the 2D sinogram prereconstruction. The PET images are reconstructed using the Ordered Subset Expectation Maximization (OSEM). We demonstrate through simulations with images contaminated by Poisson noise that the performance of the proposed method substantially surpasses that of recently published methods, both visually and in terms of statistical measures.
Closed loop control of hysteretic magnetization

This paper presents a novel method for closed loop control of magnetization of ferrite material using concepts from nonlinear control theory. A first principle nonlinear mathematical model of magnetization has been developed that describes the temporal dynamics of the magnetization process. Using concepts from nonlinear control theory, it is shown that the developed control system is able to maintain the magnetization of the structure at a desired level in the presence of arbitrary ambient excitation. Simulation of the nonlinear controller, and experimental PID studies are presented to illustrate the proposed method of closed loop control of magnetization.
The relative effect of carotid plaque heterogeneity and echogenicity on ipsilateral cerebral infarction and symptoms of cerebrovascular disease.

OBJECTIVE To determine the relative effect of carotid plaque heterogeneity and echogenicity as measured by computer on the incidence of ipsilateral cerebral infarction and symptoms of cerebrovascular disease. MATERIALS AND METHODS 138 patients with 209 carotid plaques producing > 50% stenosis were studied. 110 plaques were from symptomatic sides. All patients had computer tomography (CT) brain scans. Images of the carotid plaques obtained by duplex scanner were transferred to a computer. Using an image analysis program plaque echogenicity, measured as the grey scale median (GSM) and heterogeneity, evaluated as the heterogeneity index (HI) was calculated. RESULTS 42% of plaques were associated with cerebral infarction. Symptomatic plaques and those associated with cerebral infarction were more echolucent and less heterogeneous than asymptomatic plaques and those not associated with cerebral infarction. Plaques with GSMs below or equal to 32 were associated with a significantly higher incidence of cerebral infarction. Plaques with GSMs below or equal to 32 were associated with a significantly higher incidence of cerebral infarction and symptoms of cerebrovascular disease than those with GSMs above this level (p < 0.01). Plaques with HIs below or equal to 20 were associated with a significantly higher incidence of symptoms of cerebrovascular disease as compared to those with HIs above this level (p < 0.01). CONCLUSIONS Computer analysis of carotid plaque morphology is an objective tool that can identify high risk plaques. The potential of such analysis in the identification of asymptomatic patients with advanced carotid stenosis at a high risk of stroke should be explored in a natural history study.
Active manipulator motion planning for planetary landform awareness

This paper presents an active motion planning approach for a robotic manipulator operating in planetary surface exploration missions. A monocular camera is employed to examine the visually salient regions in an image, from which landforms of potential interests are extracted, and two key metrics are established to evaluate the information richness of the landforms. A next-best view manipulator motion planning is proposed, in which motions of the manipulator are actively planned to reach a better viewpoint to view the target landform, the safety of such operation is guaranteed by real-time estimating the relative distance using ORriented Brief (ORB)–simultaneous localization and mapping. The proposed active motion planning method is validated by an experimental trial, results from which demonstrate that it is able to safely acquire a better viewing of a just-detected planetary landform with full autonomy.
Functions of autophagy in normal and diseased liver

Autophagy has emerged as a critical lysosomal pathway that maintains cell function and survival through the degradation of cellular components such as organelles and proteins. Investigations specifically employing the liver or hepatocytes as experimental models have contributed significantly to our current knowledge of autophagic regulation and function. The diverse cellular functions of autophagy, along with unique features of the liver and its principal cell type the hepatocyte, suggest that the liver is highly dependent on autophagy for both normal function and to prevent the development of disease states. However, instances have also been identified in which autophagy promotes pathological changes such as the development of hepatic fibrosis. Considerable evidence has accumulated that alterations in autophagy are an underlying mechanism of a number of common hepatic diseases including toxin-, drug- and ischemia/reperfusion-induced liver injury, fatty liver, viral hepatitis and hepatocellular carcinoma. This review summarizes recent advances in understanding the roles that autophagy plays in normal hepatic physiology and pathophysiology with the intent of furthering the development of autophagy-based therapies for human liver diseases.
T Cell Activation + Inhibit CD4 MHC Class II ''Dimer of Dimers'' Interface Amino Acid Substitutions in the Putative

1 Activation of T lymphocytes is dependent on multiple ligand-receptor interactions. The possibility that TCR dimerization con-tributes to T cell triggering was raised by the crystallographic analysis of MHC class II molecules. The MHC class II molecules associated as double dimers, and in such a way that two TCR (and two CD4 molecules) could bind simultaneously. Several subsequent studies have lent support to this concept, although the role of TCR cross-linking in T cell activation remains unclear. Using DRA cDNAs modiﬁed to encode two different C-terminal tags, no evidence of constitutive double dimer formation was obtained following immunoprecipitation and Western blotting from cells transiently transfected with wild-type DRB and tagged DRA constructs, together with invariant chain and HLA-DM. To determine whether MHC class II molecules contribute actively to TCR-dependent dimerization and consequent T cell activation, panels of HLA-DR1 b and H2-E k cDNAs were generated with mutations in the sequences encoding the interface regions of the MHC class II double dimer. Stable DAP.3 transfectants expressing these cDNAs were generated and characterized biochemically and functionally. Substitutions in either interface region I or III did not affect T cell activation, whereas combinations of amino acid substitutions in both regions led to substantial inhibition of proliferation or IL-2 secretion by human and murine T cells. Because the amino acid-substituted molecules were serologically indistinguishable from wild type, bound antigenic peptide with equal efﬁciency, and induced Ag-dependent CD25 expression indicating TCR recognition, the reduced ability of the mutants to induce full T cell activation is most likely the result of impaired double dimer formation. These data suggest that MHC class II molecules, due to their structural properties, actively contribute to TCR cross-linking. The Journal of Immunology, 2001, 166: 800–808. m g) cDNAs. Three days after transfection, the cells were stained with pu-riﬁed L243 at saturating levels (1 m g/1 3 10 5 cells) and a FITC-conjugated goat anti-mouse mAb and analyzed by ﬂow cytometry. The expression levels of the MHC class II molecules are presented as mean ﬂuorescence intensity (MFI). Mock transfected HeLa cells transfected with pCMU plas- mid only (25 m g) were used as negative control for the MHC class II expression. Similar results were obtained when the cells were stained with the mAbs 7-4.1, DA6 231, and CA2.
Rapid Detection of Cell-Free Mycobacterium tuberculosis DNA in Tuberculous Pleural Effusion

ABSTRACT Tuberculous pleurisy is one of the most common types of extrapulmonary tuberculosis, but its diagnosis remains difficult. In this study, we report for the first time on the detection of cell-free Mycobacterium tuberculosis DNA in pleural effusion and an evaluation of a newly developed molecular assay for the detection of cell-free Mycobacterium tuberculosis DNA. A total of 78 patients with pleural effusion, 60 patients with tuberculous pleurisy, and 18 patients with alternative diseases were included in this study. Mycobacterial culture, the Xpert MTB/RIF assay, the adenosine deaminase assay, the T-SPOT.TB assay, and the cell-free Mycobacterium tuberculosis DNA assay were performed on all the pleural effusion samples. The cell-free Mycobacterium tuberculosis DNA assay and adenosine deaminase assay showed significantly higher sensitivities of 75.0% and 68.3%, respectively, than mycobacterial culture and the Xpert MTB/RIF assay, which had sensitivities of 26.7% and 20.0%, respectively (P < 0.01). All four of these tests showed good specificities: 88.9% for the adenosine deaminase assay and 100% for the remaining three assays. The T-SPOT.TB assay with pleural effusion showed the highest sensitivity of 95.0% but the lowest specificity of 38.9%. The cell-free Mycobacterium tuberculosis DNA assay detected as few as 1.25 copies of IS6110 per ml of pleural effusion and showed good accordance of the results between repeated tests (r = 0.978, P = 2.84 × 10−10). These data suggest that the cell-free Mycobacterium tuberculosis DNA assay is a rapid and accurate molecular test which provides direct evidence of Mycobacterium tuberculosis etiology.
Investigation of ambulatory blood pressure monitoring data editing criteria.

Ambulatory blood pressure monitoring (ABPM) allows multiple measurements of BP over a 24h period but often produces a proportion of measurements which are distant from the mean and unlikely to be genuine. The software which accompanies the SpaceLabs 90207 monitor requires criteria to be set which excludes outliers (autoediting) but there is no published data evaluating at what levels these autoediting criteria should be set. This study set out to ascertain whether outlying BP measurements represent true blood pressures or machine error, the determinants of machine error and appropriate autoedit criteria. Twenty subjects, both normotensive and hypertensive, were studied by ABPM with a blinded observer recording BP using a standard mercury sphygmomanometer (SMS) connected via a T-tube during three activity phases: sitting with cuffed arm still, walking but keeping arm still during measurement and sitting but gently moving the arm during measurement. The results show that a high ABPM/SMS discrepancy is associated with arm movement. After studying different autoediting criteria which aimed to eliminate the maximum number of conflicting readings while excluding the minimum number of unconflicting readings, an autoediting threshold of between 1.75 and 2 s.d. s from the mean based separately on sleep and awake measurements is suggested.
Suppression of Sleep-Induced Growth Hormone Secretion by Anticholinergic Agent Abolishes Dawn Phenomenon

The dawn phenomenon was evaluated in eight C-peptide-negative type I (insulin-dependent) diabetic patients on two occasions by measuring glucose concentrations every 30 min from 2400 to 0800 h while the subjects were receiving an insulin infusion (0.12 mU·kg−1 · min−1). In random order at 2230 h, they orally received either a sleeping medication alone or with 5.0 mg methscopolamine bromide, an anticholinergic agent. The peak growth hormone (GH) concentrations (ng/ml ± SE) after sleep were markedly inhibited by methscopolamine (4.7 ± 2.6 vs. 23.0 ± 9.2). During the control night, the late (0400–0800 h) glucose response (area under curve but above 0400 h value) was significantly higher (P < .02) than the early (2400–0400 h) glucose response (area under curve but above 2400 h value). After methscopolamine, the early and late glucose responses were virtually identical. The anticholinergic agent did not affect glucagon levels, overnight urinary catecholamine excretion, or the diurnal cortisol concentrations. The total area under the free fatty acid (FFA) curves was significantly (P < .05) reduced by methscopolamine. We conclude that sleep-induced GH secretion may cause the dawn phenomenon by increasing FFA levels. Oral administration of methscopolamine at bedtime is a simple pharmacological approach that could test the clinical importance of the dawn phenomenon.
Thermodynamic Analysis of Water Interaction with Excipient Films

The interaction of water with excipients that can form moisture-protective coatings was examined earlier by the application of theoretical models. In this study, thermodynamic analysis of water-excipient film systems has been performed to elucidate the mechanistic details of the water-excipient interaction. Partial molal free energies, enthalpies, and entropies were computed for films of lipidic (glyceryl behenate, GB) and polymeric (polyvinyl alcohol, PVA) coating excipients using the temperature dependence of the adsorption process. The analysis of free energy changes showed that excipient films were not inert participants in the water sorption process. The isoteric heats of adsorption confirmed that water formed hydrogen bonds with the excipient films and allowed estimation of number of hydrogen bonds per water molecule. This result also provided the reason for hysteresis during drying. A comparative evaluation of the application of theoretical models and thermodynamic analysis revealed that results obtained from both approaches were not always complementary. An exponential relationship was found to exist between sorption microrate constants and water activity for the PVA films at all temperatures.