Miracle-dz/UniFAR-FTUs huggingface dataset:
Safety and immunogenicity of the Na-GST-1 hookworm vaccine in Brazilian and American adults

Necator americanus Glutathione-S-Transferase-1 (Na-GST-1) plays a role in the digestion of host hemoglobin by adult N. americanus hookworms. Vaccination of laboratory animals with recombinant Na-GST-1 is associated with significant protection from challenge infection. Recombinant Na-GST-1 was expressed in Pichia pastoris and adsorbed to aluminum hydroxide adjuvant (Alhydrogel) according to current Good Manufacturing Practice. Two Phase 1 trials were conducted in 142 healthy adult volunteers in the United States and Brazil, first in hookworm-naïve individuals and then in residents of a N. americanus endemic area in Brazil. Volunteers received one of three doses of recombinant Na-GST-1 (10, 30, or 100 μg) adjuvanted with Alhydrogel, adjuvanted with Alhydrogel and co-administered with an aqueous formulation of Glucopyranosyl Lipid A (GLA-AF), or the hepatitis B vaccine. Vaccinations were administered via intramuscular injection on days 0, 56, and 112. Na-GST-1/Alhydrogel was well tolerated in both hookworm-naïve and hookworm-exposed adults, with the most common adverse events being mild to moderate injection site pain and tenderness, and mild headache and nausea; no vaccine-related severe or serious adverse events were observed. Antigen-specific IgG antibodies were induced in a dose-dependent fashion, with increasing levels observed after each vaccination in both trials. The addition of GLA-AF to Na-GST-1/Alhydrogel did not result in significant increases in specific IgG responses. In both the US and Brazil studies, the predominant IgG subclass induced against Na-GST-1 was IgG1, with lesser amounts of IgG3. Vaccination of both hookworm-naïve and hookworm-exposed adults with recombinant Na-GST-1 was safe, well tolerated, and resulted in significant antigen-specific IgG responses. Based on these results, this vaccine will be advanced into clinical trials in children and eventual efficacy studies. Trial registration ClinicalTrials.gov (NCT01261130 for the Brazil trial and NCT01385189 for the US trial)
Predicting Relevance Scores for Triples from Type-Like Relations using Neural Embedding - The Cabbage Triple Scorer at WSDM Cup 2017

The WSDM Cup 2017 Triple scoring challenge is aimed at calculating and assigning relevance scores for triples from type-like relations. Such scores are a fundamental ingredient for ranking results in entity search. In this paper, we propose a method that uses neural embedding techniques to accurately calculate an entity score for a triple based on its nearest neighbor. We strive to develop a new latent semantic model with a deep structure that captures the semantic and syntactic relations between words. Our method has been ranked among the top performers with accuracy - 0.74, average score difference - 1.74, and average Kendall's Tau - 0.35.
Cranial radiation for pediatric T‐lineage acute lymphoblastic leukemia: A systematic review and meta‐analysis

There are heterogeneous approaches to cranial radiation therapy (CRT) for T‐lineage acute lymphoblastic leukemia (T‐ALL). We performed a systematic review of studies that specified a radiation strategy and reported survival for pediatric T‐ALL. Our analysis included 62 publications reporting 78 treatment groups (patient n = 5844). The average event‐free survival (EFS) was higher by 6% per 5 years (P < 0.001). Adjusting for year, EFS differed by radiation strategy. Compared to the reference group (CRT for all) which had a year‐adjusted EFS of 65% (95% confidence interval, CI: 61–69%) the adjusted EFS was significantly worse (rate difference (RD) = −9%, 95% CI: −15 to −2%) among studies that used a risk‐directed approach to CRT (P = 0.004). The adjusted EFS for the other strategies were not significantly different compared to the reference group: CRT for central nervous system positive patients only (RD = −3%, 95% CI: −14 to 7%, P = 0.49); CRT omitted for all patients (RD = 5%, 95% CI: −4 to 15%, P = 0.33). CRT may not be necessary with current chemotherapy for T‐ALL. These findings, however, are susceptible to bias and caution should be applied in drawing conclusions on the comparative effectiveness of alternative CRT strategies. Am. J. Hematol. 89:992–997, 2014. © 2014 Wiley Periodicals, Inc.
The Effect of Low Glucose Degradation Product Dialysis Solution on Epithelial-To-Mesenchymal Transition in Continuous Ambulatory Peritoneal Dialysis Patients

♦ Background Human peritoneal mesothelial cells (HPMCs) undergo a transition from an epithelial phenotype to a mesenchymal phenotype (EMT) during peritoneal dialysis (PD). That transition may be directly related to failure of peritoneal membrane function. ♦ Objective In a randomized prospective controlled study, we investigated the effect of low glucose degradation product (GDP) dialysis solution on the transition of HPMCs. ♦ Methods Among new continuous ambulatory PD patients, 60 patients completed a 12-month protocol (low-GDP solution group, n = 32; high-GDP solution group, n = 28). At the 1st, 6th, and 12th months, HPMCs drained from overnight effluent were cultured on T25. When they had nearly reached confluence, cell scores were measured blindly by the same person (score 1 = cobblestone-shaped HPMCs, score 2 = mixed, score 3 = fibroblast dominant). Cell scores and clinical indices, including peritoneal markers, were compared between the low-GDP and high-GDP groups at the 1st, 6th, and 12th months. The factors associated with EMT were analyzed with generalized estimating equations using STATA 7.0 (STATA Corp., College Station, Texas, USA). In addition, vimentin and cytokeratin 8/18 stains were used to verify EMT in cultured cells and peritoneal specimens in some patients. ♦ Results (1) The low-GDP group showed higher dialysate cancer antigen 125 levels from the 1st to 12th months (55.4 ± 24.8 vs 8.8 ± 1.7, 56.7 ± 28.1 vs 22.1 ± 11.3, and 54.2 ± 28.2 vs 24.6 ± 16.5 U/mL, at the 1st, 6th, and 12th months, respectively; all p = 0.000). (2) The low-GDP group showed lower cell scores at the 1st, 6th, and 12th months (1.22, 1.22, and 1.56 vs 1.61, 1.75, and 2.14; p < 0.05, p < 0.01, and p < 0.01, respectively). (3) At the 12th month, the number of fibroblast-dominant cultures (score 3) was significantly lower in the low-GDP group [4/32 (12.5%) patients vs 14/28 (50%), p < 0.05]. (4) Both cobblestone-shaped HPMCs and fibroblastoid cells were positively stained with cytokeratin and vimentin. (5) There were many cytokeratin- and vimentin-positive cells in the submesothelial area in the peritoneal biopsy specimens. (6) The consistent factor associated with EMT was only high-GDP solution (60 patients, n = 178, beta coefficient 0.312, p = 0.000; 46 patients, n = 137, beta coef: 0.228, p = 0.000) and not numbers of peritonitis episodes, duration of angiotensin-converting enzyme inhibitor and/or angiotensin receptor blocker medication, or diabetes. ♦ Conclusion Low-GDP solution showed beneficial effects such as rapid remesothelialization and less EMT in the peritoneum with time on PD.
(2,9-Dimethyl-1,10-phenanthroline-κ2 N,N′)bis(2-hydroxybenzoato-κO)copper(II)

The CuII atoms in the two independent molecules of the title compound, [Cu(C7H5O3)2(C14H12N2)], are each coordinated by a bidentate 2,9-dimethyl-1,10-phenanthroline (dmphen) molecule and two monodentate 2-hydroxybenzoate anions in a distorted tetrahedral geometry. The crystal packing is stabilized by intramolecular hydrogen bonding and π–π interactions between the dmphen rings of neighboring molecules, with distances between their ring planes of 3.5670 (4) and 3.5181 (9) Å.
Mode converters in overmoded circular waveguide for a 250 GHz CARM source

A mode conversion from the TE<inf>10</inf> in WR3 rectangular waveguide to the TE<inf>53</inf> in an oversized circular waveguide is required to perform the cold test of a Bragg resonator for a high power 250 GHz Cyclotron Auto-Resonance Maser (CARM) under development at the Frascati ENEA research centre. High operating frequency and oversized cylindrical cavity (exceeding any previous CARM experiment) make the design particularly challenging. An initial transition, aimed at transforming the TE<inf>10</inf> in WR3 rectangular waveguide into the TE<inf>11</inf>, in a circular waveguide, is performed using a linear taper, a well-assessed device. Then a serpentine mode converter in circular waveguide with average radius of 1.48 mm and appropriate length has been adopted for the conversion into the TE<inf>01</inf> mode. The subsequent transducers, for not-rotating (TE<inf>0m</inf>) and rotating (TE<inf>53</inf>) modes, are provided by means of rippled wall mode converters with small periodic perturbations of waveguide radius. The ripple period corresponds approximately to the beat wavelength (X<inf>b</inf> = 2π/(β<inf>m</inf> − β<inf>n</inf>)) with β<inf>m</inf> and β<inf>n</inf> the wavenumbers of the two interacting modes. For not-rotating modes an efficient conversion has been obtained by cascading single step TE<inf>01</inf> to TE<inf>02</inf>, TE<inf>02</inf> to TE<inf>03</inf> and TE<inf>03</inf> to TE<inf>04</inf> mode converters. For each of them, profiles, radius and number of periods have been optimized in order to obtain low levels of unwanted spurious modes, low reflection and sufficient bandwidth. As the modes to convert (TE<inf>04</inf> to TE<inf>53</inf>) present similar wavenumbers, the most appropriate method for the conversion consists in a three-periods rippled-wall mode converter made by a helically corrugated waveguide with an average radius of 4 mm and five azimuthal variations. The longitudinal profile of the ripples has been properly smoothed to avoid steps between the circular waveguide and the corrugated converter. In this paper the conversion chain will be described and the simulation results (S-parameters, efficiency and electric field) of each single converter will be reported. Finally some considerations about the high precision micromachining technique to be used, such as copper electroforming and Computer Numerically Controlled (CNC) milling, for the construction of these high frequency components, will be presented.
A Novel Substrate Integrated Coaxial Line (SICL) for Wide-Band Applications

A novel substrate integrated coaxial line (SICL) is presented and experimentally verified in this paper. This structure exhibits unimodal operation over a wide frequency band, is shielded and not dispersive, can be fabricated by using simple, low-cost thin-film process, and can be easily integrated with active devices. Moreover, the same technology results particularly suitable to realize passive components for wideband applications. The design of a Lange coupler with operation bandwidth of 66%, to be used in a multi-port impulse radio for ultra wide band applications, is reported
Neutralizing antibodies can initiate genome release from human enterovirus 71

Significance Enterovirus 71 (EV71) causes yearly outbreaks of hand, foot, and mouth disease in Southeast Asian countries including China and Malaysia. Some of the infected children develop encephalitis that can be fatal or result in permanent brain damage. There are no anti-EV71 therapeutic agents available. Here it is shown that an antibody that had been generated by using an immature EV71 virus as an antigen induced the release of genome from EV71 virions, rendering the virus noninfectious. The induction of genome release is a mechanism by which antibodies can neutralize viruses. Furthermore, the approach presented in the paper could be used to prepare antibodies with similar properties against related viruses that include significant human pathogens. Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid structure of these empty particles is different from that of the mature virus and is similar to “A” particles encountered when picornaviruses recognize a potential host cell before genome release. The monoclonal antibody E18, generated by this immunization, induced a conformational change when incubated at temperatures between 4 °C and 37 °C with mature virus, transforming infectious virions into A particles. The resultant loss of genome that was observed by cryo-EM and a fluorescent SYBR Green dye assay inactivated the virus, establishing the mechanism by which the virus is inactivated and demonstrating that the E18 antibody has potential as an anti-EV71 therapy. The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated. Furthermore, the present results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles.
Purification of Chloroplasts by a Density Technique.

10. MAHLER, H. R. , MACKLER, B. , and GREEN, D. E. Studies on metalloflavoproteins. III. Aldehyde oxidase: A molybdoflavoprotein. Jour. Biol. Chem. 210: 465-480. 1954. 11. LATIMER, W. H. and HILDEBRAND, J. H. Reference Book of Inorganic Chemistry, rev. ed. Pp. 1-357. Macmillan Co., New York. 1940. 12. RIcHERT, D. A. and WESTERFELD, W. V. The relationship of iron to xanthine oxidase. Jour. Biol. Chem. 209: 179-189. 1954.
Electrical Compensation of OLED Luminance Degradation

This letter presents a stable compensation scheme for active-matrix organic light-emitting-diode (AMOLED) displays based on the observed strong interdependence between the luminance degradation of organic light-emitting diodes (OLEDs) and its current drop under bias stress. This feedback-based compensation provides 30% improvement in luminance stability under 1600 h of accelerative stress. To employ this scheme in AMOLED displays, a new pixel circuit is presented that provides on-pixel electrical access to the OLED current without compromising the aperture ratio.
A novel 2.4GHz LNA with digital gain control using 0.18/spl mu/m CMOS

This paper presents a novel gain control method for 2.4 GHz LNA applications. In this design, a digital mode gain control concept was implemented. This chip can accept an appropriate control bit that come from base band to achieve power saving. With digital mode gain control to achieve low noise and high 1dB compression point simultaneously without increasing any circuit and power consumption. Depend on receiving signal strength, there are four gain modes can be selecting automatically. The compact CMOS LNA is optimized for low-power-consuming ISM band applications and is fabricated using commercial 0.18/spl mu/m CMOS process. With current re-use technology, the power consumption and linearity can be optimizing. The fully integrated 2.4GHz gain controllable LNA exhibits 15.2dB maximum gain, 4.1dB minimum gain, respectively. Also, the LNA has excellent noise performance at high gain mode; 1.55dB of noise figure is achieved in this work. The digital modes gain controllable LNA produces a 1-dB compression output power of -10 dBm. It consumes 2.5mA current from a supply voltage of 1.8V.
Bipolar II disorder is associated with thinning of prefrontal and temporal cortices involved in affect regulation

The neurobiological substrate of bipolar II disorder (BD‐II) remains largely unknown. A few previous studies have found evidence for cerebral cortical thinning in mixed samples of BD‐II and bipolar I disorder patients; however, no study of cortical thickness or surface area has been limited to BD‐II. In the present study, we compared magnetic resonance imaging (MRI)‐based indices of cortical thickness and surface area between individuals with BD‐II and healthy controls.
Protein-facilitated base flipping in DNA by cytosine-5-methyltransferase

DNA methylation, various DNA repair mechanisms, and possibly early events in the opening of DNA as required for transcription and replication are initiated by flipping of a DNA base out of the DNA double helix. The energetics and structural mechanism of base flipping in the presence of the DNA-processing enzyme, cytosine 5-methyltransferase from HhaI (M.HhaI), were obtained through molecular dynamics based upon free-energy calculations. Free-energy profiles for base flipping show that, when in the closed conformation, M.HhaI lowers the free-energy barrier to flipping by 17 kcal/mol and stabilizes the fully flipped state. Flipping is shown to occur via the major groove of the DNA. Structural analysis indicates that flipping is facilitated by destabilization of the DNA double-helical structure and substitution of DNA base-pairing and base-stacking interactions with DNA–protein interactions. The fully flipped state is stabilized by DNA–protein interactions that are enhanced upon binding of coenzyme. This study represents an atomic detail description of the mechanism by which a protein facilitates specific structural distortion in DNA.
Immunolocalization of Type I or Type II Activin Receptors in the Rat Brain

We have studied immunolocalization of activin receptors in the central nervous system using polyclonal antibodies (IgG) to type I (50–55 kDa, ActRI), type II (70–75 kDa, ActRII) or a subtype of type II known as type IIB (ActRIIB) receptors of activin. A total of 7 antisera to rat activin receptors was generated, i.e. 3 kinds of antisera to the extracellular domain (ActRI(81–89), ActRII(91–100), or ActRIIB(90–99)) and 4 antisera to the kinase domain (ActRI(323–333), ActRII(307–319), ActRII(407–420) or ActRIIB(306–319)). The region of aa 407–420 of ActRII is identical with that of ActRIIB. At first, we characterized these antibodies by Western blot analysis using ovarian proteins fractionated by preparative SDS‐PAGE. All antibodies to ActRII and ActRIIB specifically reacted with 75 kDa‐proteins which could also bind to activin‐A. Anti‐ActRII(91–100) antibody also reacted with 62 kDa‐proteins which were capable of binding with activin‐A. Although no positive reactions to anti‐ActRI(81–89) antibody were seen in ovarian proteins, a positive reaction was detected at 52 kDa only when the proteins were deglycosylated. By use of these antibodies, immunolocalization of activin receptors was examined in the rat brain. The patterns of expression of activin type I and type II receptors were different. Positive reactions to anti‐ActRII(91–100) antibody were detected in neurons of the cerebral cortex, hippocampus, medial amygdala and thalamus. In the hypothalamus, some neurons of the supraoptic nucleus were weakly stained, and widely scattered neurons of the lateral hypothalamic area were moderately stained. On the contrary, the most intense reactions to anti‐ActRI(81–89) antibody were detected in neurons of the lateral hypothalamic area. In addition, many neurons of the cerebral cortex were also stained, but neurons of the hippocampus and the amygdala were not stained. These results suggest that activin may have physiological roles not only for hypothalamic neuroendocrinological and feeding‐related systems as suggested previously but may also have functions in cortical and limbic pathways as a neuromodulator or for maintenance of neurons.
Consensus-based distributed droop control of VSGs for isolated AC micro-grids by ADMMs

High penetration of distributed interface converters (DICs) in the isolated AC micro-grid (MG) may result in poor frequency and voltage response due to low inertia of conventional DICs. In order to overcome this difficulty, the concept of the virtual synchronous generators was proposed recently. meanwhile, in order to improve the convergence rate of the conventional consensus-based droop control and resilience to noises, new implementation of consensus-based distributed droop control by Alternating Direction Multipliers Methods (AD-MMs) is proposed. Possible extensions of the proposed method to two variants, including consensus ADMM with double message exchange and unique message exchange, are also discussed. Numerical experiments of a 14-bus/6-DIC micro-grid system on real-time digital simulators are performed to validate the effectiveness of the proposed distributed droop control mechanism.
Bacteriological analysis of different foods to determine the fitness for human consumption.

A total of 230 food samples were collected over a period of 18 months from different shops, vendors and canteens of Rawalpindi/Islamabad region. The sample included cooked, uncooked, frozen and non frozen (pre-packed) food. One hundred and five samples were found contaminated with different bacterial strains, which were grown at two different temperatures i.e. 35-37°C and at room temperature. The pathogenic microorganisms isolated were identified as Coliforms group, 79 (75.23%), Escherichia coli, 49 (46.66%), Staphylococcus aureus, 11(10.47%), Clostridium perfringens, 5 (4.85%)and Salmonella spp, 1 (0.9%). Other pathogens namely, Shigella, Vibrio cholerae and Vibrio parahaemolyticus were not isolated (JPMA 35: 79, 1985).
IFN‐gamma priming of adipose‐derived stromal cells at “physiological” hypoxia

Multipotent mesenchymal stromal cells (MSCs) are considered cue regulators of tissue remodeling. Their activity is strongly governed by local milieu, where O2 level is most important. The elevation of inflammatory mediators and acute O2 lowering may additionally modulate MSC activity. In present paper the priming effects of IFN‐gamma on adipose tissue‐derived MSCs (ASCs) at tissue‐related O2 level (5%) and acute hypoxic stress (0.1% O2) were assessed as alterations of ASCs’ CFU‐F, proliferation, migration, osteo‐commitment. IFN‐gamma priming provoked ROS elevation, cell growth slowdown, attenuation of both spontaneous and induced osteodifferentiation of tissue O2‐adapted ASCs. The prominent changes in ASC cytoskeleton‐related gene transcription was detected. IFN‐gamma exposure shifted the ASC paracrine profile, suppressing the production of VEGF and IL‐8, while MCP‐1 and IL‐6 were stimulated. Conditioned medium of IFN‐gamma‐primed ASCs did not activate vessel growth in the CAM assay, but induced endothelial cell migration in “wound closure. ” Short‐term hypoxia suppressed CFU‐F number, IFN‐gamma‐induced elevation of IL‐6 and endothelial cell migration, while it abolished IFN‐gamma‐provoked VEGF inhibition. After N‐acetyl cysteine treatment ROS level was partly abolished providing additional enhancement of IL‐6 and suppression of IL‐8 and VEGF production. These findings demonstrated that paracrine activity of ASCs in part may be governed by ROS level. Thus, this study first demonstrated that IFN‐gamma priming itself and in combination with acute O2 deprivation could supply dual effects on ASC functions providing both stimulatory and hampering effects. The equilibrium of these factors is a substantial requirement for the execution of MSC remodeling functions.
The Design and Use of Steerable Filters

The authors present an efficient architecture to synthesize filters of arbitrary orientations from linear combinations of basis filters, allowing one to adaptively steer a filter to any orientation, and to determine analytically the filter output as a function of orientation. Steerable filters may be designed in quadrature pairs to allow adaptive control over phase as well as orientation. The authors show how to design and steer the filters and present examples of their use in the analysis of orientation and phase, angularly adaptive filtering, edge detection, and shape from shading. One can also build a self-similar steerable pyramid representation. The same concepts can be generalized to the design of 3-D steerable filters. >