Miracle-dz/UniFAR-FTUs huggingface dataset:
In silico study of fragile histidine triad interaction domains with MDM2 and p53

Background: Fragile histidine triad (FHIT) is considered as a member of the histidine triad (HIT) nucleotide-binding protein superfamily regarded as a putative tumor suppressor executing crucial role in inhibiting p53 degradation by MDM2. Accumulating evidences indicate FHIT interaction with p53 or MDM2; however, there is no certain study deciphering functional domains of FHIT involving in the interaction with MDM2 and/or p53. In this regard, such evident interaction can spring in mind determining important domains of FHIT binding to MDM2 with regard to p53. Materials and Methods: Since there were not any previous studies appraising complete three-dimensional structures of target molecules, molecular modeling was carried out to construct three-dimensional models of full FHIT, MDM2, P53 and also FHIT segments. Truncated structures of FHIT were created to reveal critical regions engaging in FHIT interaction. Results: Given the shape and shape/electrostatic total energy, FHIT structures (β1-5), (β3-7, α1), and (β5-7, α1) appeared to be better candidates than other structures in interaction with full MDM2. Furthermore, FHIT structures (β6-7), (β6-7, α1), (β4-7, α1) were considered to be better than other structures in interaction with p53. FHIT truncates that interact with MDM2 presented lower energy levels than FHIT truncates interacting with p53. Conclusion: These findings are beneficial to understand the mechanism of the FHIT-MDM2-p53 complex activation for designing inhibitory compounds.
Biases in Understanding Attention Deficit Hyperactivity Disorder and Autism Spectrum Disorder in Japan

Recent research has shown high rates of comorbidity between attention deficit hyperactivity disorder (ADHD) and autism spectrum disorder (ASD) and difficulties regarding differential diagnosis. Unlike those in Western countries, the Japanese ADHD prevalence rate is lower relative to that of ASD. This inconsistency could have occurred because of cultural diversities among professionals such as physicians. However, little is known about attitudes toward ADHD and ASD in non-Western cultural contexts. We conducted two experiments to identify biases in ASD and ADHD assessment. In Study 1, we examined attitudes toward these disorders in medical doctors and mental health professionals, using a web-based questionnaire. In Study 2, medical doctors and clinical psychologists assessed four fictional cases based on criteria for ADHD, ASD, oppositional defiant disorder, and disinhibited social engagement disorder (DSED). Diagnosis of ASD was considered more difficult relative to that of ADHD. Most participants assessed the fictional DSED case as ASD, rather than DSED or ADHD. The results provide evidence that Japanese professionals are more likely to attribute children’s behavioral problems to ASD, relative to other disorders. Therefore, Japanese therapists could be more sensitive to and likely to diagnose ASD, relative to therapists in other countries. These findings suggest that cultural biases could influence clinicians’ diagnosis of ADHD and ASD.
Management of dental patients receiving antiplatelet therapy or chronic oral anticoagulation: A review of the latest evidence

Abstract The perioperative management of patients treated with antithrombotic medications who undergo surgical procedures represents a common clinical problem. Dental interventions are usually associated with a low risk of bleeding; however, the dental implications of new antithrombotic agents are not yet fully understood. The present review is based on the latest evidence and recommendations published on the periprocedural management of dental patients treated with single or dual antiplatelet therapy, vitamin K antagonists, or direct oral anticoagulants for a variety of indications.
Proximal Aortic Surgery: Upper "J" or Conventional Sternotomy?

BACKGROUND While minimally invasive procedures are being used in cardiac surgery, experience with minimally invasive proximal aortic surgery has been limited to certain centers. METHODS Between January 2010 and March 2015, 54 patients with an upper "J" hemi-sternotomy and 75 patients with a conventional sternotomy due to proximal aortic pathology were included in this study. Forty-five patients from the "J" hemi-sternotomy group were matched with 45 patients from the conventional sternotomy group with respect to age, sex, ejection fraction, diabetes, hypertension, smoking history and operative type. Perioperative variables were in-hospital mortality, surgery for revision, amount of blood loss, requirement for blood transfusion, cardiopulmonary bypass (CPB), aortic cross-clamp and unilateral cerebral protection times, duration of ventilation, and length of intensive care unit (ICU) and total hospital stay. RESULTS Patients were between 21-76 years with a mean age of 58.14 ± 11.06 years; 73.3% (n = 66) were male and 26.7% (n = 24) were female. Of all the cases included, 36.7% (n = 33) had isolated ascending aortic replacement, 41.1% (n = 37) had concomitant aortic valve replacement and ascending aortic replacement, and 22.2% (n = 20) had a Bentall procedure. Statistically, the amount of bleeding (P = .026), length of ventilation (P = .001), ICU (P = .001) and total hospital stay (P = .004) in the "J" hemi-sternotomy group were all found to be significantly lower than those in the conventional group. CONCLUSIONS Minimally invasive techniques like an upper "J" hemi-sternotomy can be safely performed without prolonging the aortic clamp time, and with less blood loss, less ventilatory support, and shorter ICU and total hospital stays when compared to conventional methods.
Detection of carrier-envelope offset frequency in an Er-doped fiber femtosecond laser

It is the inevitable trend of the development for the optical frequency comb based on the Ti: sapphire femtosecond laser to be replaced by that based on the Er: fiber femtosecond laser. The detection of the carrier-envelope offset f 0 in the femtosecond laser is a key to the realization of an optical frequency comb. In this paper, the structure of the optical part of an Er: fiber optical frequency comb is introduced. The optical pulses from an Er: fiber femtosecond laser with a repetition of 230 MHz are first amplified with a two stage amplifiers, spectrally broadened in a highly nonlinear fiber, and then pass through a single-beam f—2f self-referencing interferometer. By optimizing the optical parameters, the f 0 with a signal-to-noise ratio of 30 dB is obtained in the Er: fiber femtosecond laser, which is favorable for the realization of an Er: fiber frequency comb.
Adaptable Hybrid Filter Bank Analog-to-Digital Converters for Simplifying Wideband Receivers

The hybrid filter bank (HFB) has been considered as a promising solution for high-speed, high-resolution analog-to-digital conversion. In this letter, we propose an adaptable HFB architecture of which the conversion band can be adapted according to the frequency band of interest, e.g., the entire working band of a wideband receiver or a subband with an arbitrary spectrum position. Furthermore, we propose a strategy based on the adaptable HFB for a wideband receiver to efficiently fulfill different tasks. For spectrum sensing, the entire wideband conversion HFB is used. While for subband signal receiving, the subband conversion HFB is utilized. Examples show that compared with the existing HFB studies in which the wanted subband signal is extracted from the digitalized entire working band, the proposed strategy can significantly simplify the receiving process for subband signals, because the subband conversion HFB has a filtering effect on the input spectrum.
Passive Downhole Pressure Sensor Based on Surface Acoustic Wave Technology

A passive surface acoustic wave (SAW) pressure sensor was developed for real-time pressure monitoring in downhole application. The passive pressure sensor consists of a SAW resonator, which is attached to a circular metal diaphragm used as a pressure transducer. While the membrane deflects as a function of pressure applied, the frequency response changes due to the variation of the SAW propagation parameters. The sensitivity and linearity of the SAW pressure sensor were measured to be 8.3 kHz/bar and 0.999, respectively. The experimental results were validated with a hybrid analytical–numerical analysis. The good results combined with the robust design and packaging for harsh environment demonstrated it to be a promising sensor for industrial applications.
Modified Ehlers' index for improved detection of heart rate asymmetry in Poincaré plot

Heart rate asymmetry (HRA) is a quantifiable and visible phenomena in é plot which is defined with respect to line of identity (RR(i)=RR(i+1)). In general, third moment measure (skewness) is most appropriate for quantifying asymmetry of any time series signal. Existing Elhers' index (EI) is the third moment measure but it is defined based on the actual time series signal. The aim of this study is to redefine EI as EIR in view of stated HRA definition. The new index calculated as a third moment along the minor axis of the plot from the derived signal along this axis. To show the benefit of EIR over EI, we have calculated both index values of 54 Normal Sinus Rhythm (NSR) and 272 Arrhythmia subjects taken from Physionet NSR and Arrhythmia databases. The results showed that EIR (ROC area =0.80) discriminates NSR from Arrhythmia subjects better than EI (ROC area=0.59). This study could be useful for quantifying HRA in é plot and classifying different pathology conditions.
A state alteration and inspection-based interactive debugger

Despite significant advances in automated debugging, programmers still rely on traditional interactive debuggers (e.g., GDB) to find and fix bugs. While popular, these debuggers have major deficiencies: they do not guide the programmer in narrowing the source of error, and they only support a limited and low-level set of state-altering commands, hence semantic state alteration requires recompilation and reexecution. To address these shortcomings, we present an interactive debugger that combines capabilities that reduce debugging effort and increase debugging speed. The capabilities that yield these benefits include: state alteration commands for dynamically switching the directions of conditional branches and suppressing the execution of statements; state inspection commands including navigating and pruning dynamic slices; and state rollback and checkpointing commands to allow reexecution of the program from an earlier checkpoint. Our prototype is built on top of GDB using the Pin infrastructure; we also provide a GUI based on KDbg. Our experience shows that our debugger handles many kinds of real bugs effectively and efficiently.
Low serum levels of High-Density Lipoprotein cholesterol (HDL-c) as an indicator for the development of severe postpartum depressive symptoms

Postpartum depression (PPD) is a psychiatric complication of childbirth affecting 10–20% of new mothers and has negative impact on both mother and infant. Serum lipid levels have been related to depressive disorders, but very limited literatures are available regarding the lipid levels in women with postpartum depression. The present study is aimed to examine the association of serum lipids with the development of postpartum depressive symptoms. This is a cross sectional study conducted at a tertiary care hospital in South India. Women who came for postpartum check-up at 6th week post-delivery were screened for PPD (September 2014-October 2015). Women with depressive symptoms were assessed using EPDS (Edinburgh Postnatal Depression Scale). The study involved 186 cases and 250 controls matched for age and BMI. Serum levels of lipid parameters were estimated through spectrophotometry and the atherogenic indices were calculated in all the subjects. Low serum levels of Total Cholesterol (TC) and High Density Lipoprotein cholesterol (HDL-c) were significantly low in PPD women with severe depressive symptoms. The study recorded a significant negative correlation between HDL-c and the EPDS score in PPD women (r = -0.140, p = 0.05). Interestingly, the study also observed a significant negative correlation between Body Mass Index (BMI) and EPDS scores in case group (r = -0.146, p = 0.047), whereas a positive correlation between the same in controls (r = 0.187, p = 0.004). Our study demonstrated that low levels of serum HDL-c is correlated with the development of severe depressive symptoms in postpartum women. Study highlights the role of lipids in the development of postpartum depressive symptoms.
Neuroimages : “ Filling out ” in Cavernous Hemangioma of the Cavernous Sinus

Cavernous sinus hemangiomas, which histologically correspond to cavernous (hem)angiomas (cavernomas), are rare benign mesenchymal (extra-axial) neoplasms of the cavernous sinus (female > male, mean age ca. 40–50 years, 3% of suprasellar tumors; [1–4]). Clinical presentation is variable and depends on the size of the tumor. Headache and retrobulbar pain are frequent. Less common symptoms are cranial neuropathies due to intracavernous nerve compression, loss of visual acuity caused by compression of the optic nerve and dizziness, probably related to blood flow impairment within the cavernous sinus and/or carotid artery [1, 3, 4]. Computed tomography (CT) usually reveals erosion and bone remodeling [5], indicating slow progression. This is in contrast to calvarial hemangiomas, which generally display (trabecular) calcifications [6]. The magnetic resonance imaging (MRI) typically shows T1w isointense and T2w hyperintense, strongly contrast-enhancing extraaxial masses [2, 5]. The pivotal sign of cavernous sinus hemangiomas is the edge to center enhancement (“filling in”) in dynamic contrast-enhanced MRI [2, 7]. Due to the strong contrast enhancement the differential diagnoses of cavernous sinus hemangiomas are meningiomas and schwannomas [1, 4].
EARLY ORGAN-SPECIFIC ENDOTHELIAL ACTIVATION DURING HEMORRHAGIC SHOCK AND RESUSCITATION

Multiple organ dysfunction syndrome (MODS) is a complication of hemorrhagic shock (HS) and related to high morbidity and mortality. Interaction of activated neutrophils and endothelial cells is considered to play a prominent role in the pathophysiology of MODS. Insight in the nature and molecular basis of endothelial cell activation during HS can assist in identifying new rational targets for early therapeutic intervention. In this study, we examined the kinetics and organ specificity of endothelial cell activation in a mouse model of HS. Anesthetized male mice were subjected to controlled hemorrhage to a MAP of 30 mmHg. Mice were killed after 15, 30, 60, or 90 min of HS. After 90 min of hemorrhagic shock, a group of mice was resuscitated with 6% hydroxyethyl starch 130/0.4. Untreated mice and sham shock mice that underwent instrumentation and 90 min of anesthesia without shock served as controls. Gene expression levels of inflammatory endothelial cell activation (P-selectin, E-selectin, vascular cell adhesion molecule 1, and intercellular adhesion molecule 1) and hypoxia-responsive genes (vascular endothelial growth factor and hypoxia-inducible factor 1&agr;) were quantified in kidney, liver, lung, brain, and heart tissue by quantitative reverse-transcription-polymerase chain reaction. Furthermore, we examined a selection of these genes with regard to protein expression and localization using immunohistochemical analysis. Induction of inflammatory genes occurred early during HS and already before resuscitation. Expression of adhesion molecules was significantly induced in all organs, albeit to a different extent depending on the organ. Endothelial genes CD31 and VE-cadherin, which function in endothelial cell homeostasis and integrity, were not affected during the shock phase except for VE-cadherin in the liver, which showed increased mRNA levels. The rapid inflammatory activation was not paralleled by induction of hypoxia-responsive genes. This study demonstrated the occurrence of early and organ-specific endothelial cell activation during hemorrhagic shock, as presented by induced expression of inflammatory genes. This implies that early therapeutic intervention at the microvascular level may be a rational strategy to attenuate MODS.
A componential model for mental addition.

A componential model capable of representing simple and complex forms of mental addition was proposed and then tested by using chronometric techniques. A sample of 23 undergraduate students responded to 800 addition problems in a true-false reaction time paradigm. The 800 problems comprised 200 problems of each of four types: two single-digit addends, one single- and one double-digit addend, two double-digit addends, and three single-digit addends. The results revealed that the columnwise product of addends, a structural variable consistent with a memory network retrieval process, was the best predictor of mental addition for each of the four types of problem. Importantly, the componential model allowed estimation of effects of several other structural variables, e.g., carrying to the next column and speed of encoding of digits. High levels of explained variance verified the power of the model to represent the reaction time data, and the stability of estimates across types of problem implied consistent component use by subjects. Implications for research on mental addition are discussed.
Detection of Clostridium difficile antigen and toxin in stool specimens: Comparison of the C. difficile quik chek complete enzyme immunoassay and GeneXpert C. difficile polymerase chain reaction assay

Background/Aims: Accurate and rapid laboratory diagnosis of Clostridium difficile infections (CDI) remains a significant challenge. A two-step algorithm for detection of toxigenic C. difficile in stool based on initial screening for glutamate dehydrogenase assay followed by confirmation by toxin A+B detection using an enzyme immunoassay (EIA) or molecular assay has been proposed. We aimed to evaluate the C. difficile Quik Chek Complete® (QCC-EIA) versus the GeneXpert® C. difficile polymerase chain reaction (PCR) assay in this two-step algorithm. Materials and Methods: Two hundred and ten liquid stool samples obtained between June 2014 and June 2015 from patients suspected of CDI were tested by the QCC-EIA and GeneXpert PCR assay. The GeneXpert assay was used as the reference standard to calculate the QCC-EIA sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). Results: Of the 210 stool samples tested, 43 (20.5%) were positive by QCC-EIA, while 31 (14.8%) were positive by GeneXpert assay. The sensitivity and specificity of the QCC-EIA were found to be 100 and 93%, respectively; the PPV and NPV were 72 and 100%, respectively. The binary toxin was detected in 12 (38.7%) and tcdC gene deletion in 3 (9.6%). Conclusions: The low specificity of QCC-EIA makes it less reliable as a confirmatory test for CDI diagnosis. This test may be used as a screening test in a two-step algorithm when combined with a molecular assay or another confirmatory test.
Dietary capsaicin and its anti-obesity potency: from mechanism to clinical implications

Obesity is a growing public health problem, which has now been considered as a pandemic non-communicable disease. However, the efficacy of several approaches for weight loss is limited and variable. Thus, alternative anti-obesity treatments are urgently warranted, which should be effective, safe, and widely available. Active compounds isolated from herbs are similar with the practice of Traditional Chinese Medicine, which has a holistic approach that can target to several organs and tissues in the whole body. Capsaicin, a major active compound from chili peppers, has been clearly demonstrated for its numerous beneficial roles in health. In this review, we will focus on the less highlighted aspect, in particular how dietary chili peppers and capsaicin consumption reduce body weight and its potential mechanisms of its anti-obesity effects. With the widespread pandemic of overweight and obesity, the development of more strategies for the treatment of obesity is urgent. Therefore, a better understanding of the role and mechanism of dietary capsaicin consumption and metabolic health can provide critical implications for the early prevention and treatment of obesity.
ANTIMICROBIAL POTENTIALOF HEMOLYMPH AND HEPATOPANCREAS OFPORTUNUSSEGNISCRABS

Objective: The potential of marine crabs as a source of bioactive products is largely unexplored. Methods: Portunussegnis crabs were collected from the northern coast of Persian Gulf. Antimicrobial activities of hepatopancreas,hemolymph and proteinsof hemplymphfrom P. segnis crabs against a range of 11 different bacterial and 6 fungal pathogen strains were examined. Two positive controls Amoxicillin and Penicillin were also used. Results: The maximum(16 mm) and minimum (7.2 mm) activity was recorded frommale hemolymph and male hepatopancreas against Entrobacter sp. and Serratiamarcesences respectively. Incomparison with bacterial pathogens, fungal pathogens showed more resistance to the extracts. Conclusion: Results show that hemolymph and hepatopancreas of brachyuran crabs may have Antimicrobial activity.
Pharmacokinetics of Diclofenac and Hydroxypropyl‐β‐Cyclodextrin (HPβCD) Following Administration of Injectable HPβCD‐Diclofenac in Subjects With Mild to Moderate Renal Insufficiency or Mild Hepatic Impairment

Given their established analgesic properties, nonsteroidal anti‐inflammatory drugs (NSAIDs) represent an important postoperative pain management option. This study investigated: (1) the effects of mild or moderate renal insufficiency and mild hepatic impairment on the pharmacokinetics (PK) of diclofenac and hydroxypropyl‐β‐cyclodextrin (HPβCD) following administration of the injectable NSAID HPβCD‐diclofenac; and (2) the PK of HPβCD following administration of HPβCD‐diclofenac and intravenous itraconazole formulated with HPβCD in healthy adults. Diclofenac clearance (CL) and volume of distribution (Vz) tended to increase with decreasing renal function (moderate insufficiency versus mild insufficiency or healthy controls). Regression analysis demonstrated a significant relationship between Vz (but not CL or elimination half‐life, t½) and renal function. HPβCD CL was significantly decreased in subjects with renal insufficiency, with a corresponding increase in t½. There were no significant differences in diclofenac or HPβCD PK in subjects with mild hepatic impairment versus healthy subjects. Exposure to HPβCD in healthy subjects following HPβCD‐diclofenac administration was ∼12% of that with intravenous itraconazole, after adjusting for dosing schedule and predicted accumulation (<5% without adjustment). With respect to PK properties, these results suggest that HPβCD‐diclofenac might be administered to patients with mild or moderate renal insufficiency or mild hepatic impairment without dose adjustment (NCT00805090).