Miracle-dz/UniFAR-FTUs huggingface dataset:
Leukemia Inhibitory Factor Signaling Is Required for Lung Protection during Pneumonia

Lung infections represent a tremendous disease burden and a leading cause of acute lung injury. STAT3 signaling is essential for controlling lung injury during pneumonia. We previously identified LIF as a prominent STAT3-activating cytokine expressed in the airspaces of pneumonic lungs, but its physiological significance in this setting has never been explored. To do so, Escherichia coli was intratracheally instilled into C57BL/6 mice in the presence of neutralizing anti-LIF IgG or control IgG. Anti-LIF completely eliminated lung LIF detection and markedly exacerbated lung injury compared with control mice as evidenced by airspace albumin content, lung liquid accumulation, and histological analysis. Although lung bacteriology was equivalent between groups, bacteremia was more prevalent with anti-LIF treatment, suggestive of compromised barrier function rather than impaired antibacterial defense as the cause of dissemination. Inflammatory cytokine expression was also exaggerated in anti-LIF–treated lungs, albeit after injury had ensued. Interestingly, alveolar neutrophil recruitment was modestly but significantly reduced compared with control mice despite elevated cytokine levels, indicating that inflammatory injury was not a consequence of excessive neutrophilic alveolitis. Lastly, the lung transcriptome was dramatically remodeled during pneumonia, but far more so following LIF neutralization, with gene changes implicating cell death and epithelial homeostasis among other processes relevant to tissue injury. From these findings, we conclude that endogenous LIF facilitates tissue protection during pneumonia. The LIF–STAT3 axis is identified in this study as a critical determinant of lung injury with clinical implications for pneumonia patients.
The Anterior Temporal Approach for Microsurgical Thromboembolectomy of an Acute Proximal Posterior Cerebral Artery Occlusion

BACKGROUND: In a short window of time, intravenous and intra-arterial thrombolysis is the first treatment option for patients with an acute ischemic stroke caused by the occlusion of one of the major brain vessels. Endovascular treatment techniques provide additional treatment options. In selected cases, high revascularization rates following microsurgical thromboembolectomy in the anterior circulation were reported. A technical note on successful thromboembolectomy of the proximal posterior cerebral artery has not yet been published. OBJECTIVE: To describe the technique of microsurgical thromboembolectomy of an acute proximal posterior cerebral artery occlusion and the brainstem perforators via the anterior temporal approach. METHODS: The authors present a technical report of a successful thromboembolectomy in the proximal posterior cerebral artery. The 64-year-old male patient had an acute partial P1 thromboembolic occlusion, with contraindications for intravenous recombinant tissue plasminogen activator. The patient underwent an urgent microsurgical thromboembolectomy after a frontotemporal craniotomy. RESULTS: The postoperative computerized tomography angiography showed complete recanalization of the P1 segment and its perforators, which were previously occluded. The early outcome after 1 month and 1 year follow-ups showed improvement from modified Rankin scale 4 to modified Rankin scale 1. CONCLUSION: Microsurgical thromboembolectomy can be an effective treatment option for proximal occlusion of the posterior cerebral artery in selected cases and experienced hands. Compared with endovascular treatment, direct visual control of brainstem perforators is possible. ABBREVIATIONS: ICG, indocyanine green mRS, modified Rankin scale P1, precommunicating segment of posterior cerebral artery PCA, posterior cerebral artery r-tPA, recombinant tissue plasminogen activator
CYCLIC RED BLOOD CELL DESTRUCTION IN THALASSEMIA

A retrospective analysis of time series of hemoglobin (Hb) destruction of 24 children (11 males and 13 females) with thalassemia from the age of 6 to 12 years showed that the Hb destruction rate typically oscillated with an average period of 50 days. A possible relation between the periodism and the severity of the disease is also suggested. (Chronobiology International, 18(4), 729–736, 2001)
Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Caspofungin Susceptibility Testing of Candida and Aspergillus Species

ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus. Complete essential agreement was observed with the CLSI reference method, with categorical agreement for 94.1% of the Candida isolates tested. Thus, MALDI-TOF MS is a reliable and accurate method to detect fungal isolates with reduced caspofungin susceptibility.
Identification and Estimation of Online Price Competition with an Unknown Number of Firms

This paper considers identification and estimation of a general model for online price competition. We show that when the number of competing firms is unknown the underlying parameters of the model can still be identified and estimated employing recently developed results on measurement errors. We illustrate our methodology using UK data for personal digital assistants and employ the estimates to simulate competitive effects. Our results reveal that heightened competition has differential effects on the prices paid by different consumer segments.
Design of square generator with small look-up table

A table of squares for n-bit numbers can be used for LUT-based multiplication for n-bit numbers. This squares-based multiplication using look-up table simplifies the process as a simple addition/subtraction and table look-up, and, thus, speed-ups the operating speed. This study presents a low-cost, yet efficient square generator using a recursive scheme.
Health information literacy in everyday life: A study of Finns aged 65–79 years

This article examines the health information literacy of elderly Finns. The results are based on a survey conducted in January 2011. The questionnaire was distributed to 1000 persons that were randomly drawn from the Finnish Population Register. The respondents were aged 65–79 years (mean age 70 years) and lived in the Turku region in Finland. A total of 281 questionnaires (28%) were returned. χ2 analyses were used to find possible relationships between demographic factors, as well as interest, seeking activity, current self-rated health and different dimensions of health information literacy, including needs, seeking and use of health-related information. Significant relationships were found between education level, interest in health information, seeking activity, self-rated current health and dimensions of health information literacy. Some categories of elderly people are more vulnerable regarding obtaining and use of health information: those with lower levels of education, those with poor health, and those who are not interested in and active at seeking information. For people who are found in any of these categories, it is important that available health-related information is understandable and can be accessed without too much effort—something that information providers should take into account.
Production of 2nd generation bioethanol from lucerne - optimization of hydrothermal pretreatment.

Lucerne (Medicago sativa) has many qualities associated with sustainable agriculture such as nitrogen fixation and high biomass yield. Therefore, there is interest in whether lucerne is a suitable biomass substrate for bioethanol production, and if hydrothermal pretreatment (HTT) of lucerne improves enzymatic convertibility, providing sufficient enzymatic conversion of carbohydrate to simple sugars for ethanol production. The HTT process was optimised for lucerne hay, and the pretreated biomass was assessed by carbohydrate analysis, inhibitor characterisation of liquid phases, and by simultaneous saccharification and fermentation (SSF) of the whole slurry with Cellubrix enzymes and Saccharomyces cerevisiae yeast. The optimal HTT conditions were 205°C for 5 minutes, resulting in pentose recovery of 81%, and an enzymatic convertibility of glucan to monomeric glucose of 74%, facilitating a conversion of 6.2% w/w of untreated material into bioethanol in SSF, which is equivalent to 1,100 litre ethanol per hectare per year.
Automatic Video Captioning using Deep Neural Network

Video understanding has become increasingly important as surveillance, social, and informational videos weave themselves into our everyday lives. Video captioning offers a simple way to summarize, index, and search the data. Most video captioning models utilize a video encoder and captioning decoder framework. Hierarchical encoders can abstractly capture clip level temporal features to represent a video, but the clips are at fixed time steps. This thesis research introduces two models: a hierarchical model with steered captioning, and a Multi-stream Hierarchical Boundary model. The steered captioning model is the first attention model to smartly guide an attention model to appropriate locations in a video by using visual attributes. The Multi-stream Hierarchical Boundary model combines a fixed hierarchy recurrent architecture with a soft hierarchy layer by using intrinsic feature boundary cuts within a video to define clips. This thesis also introduces a novel parametric Gaussian attention which removes the restriction of soft attention techniques which require fixed length video streams. By carefully incorporating Gaussian attention in designated layers, the proposed models demonstrate state-of-the-art video captioning results on recent datasets.
An Efficient Parallel Coding Scheme in Erasure-Coded Storage Systems

Erasure codes have been considered as one of the most promising techniques for data reliability enhancement and storage efficiency in modern distributed storage systems. However, erasure codes often suffer from a time-consuming coding process which makes them nearly impractical. The opportunity to solve this problem probably rely on the parallelization of erasure-code-based application on the modern multi/many-core processors to fully take advantage of the adequate hardware resources on those platforms. However, the complicated data allocation and limited I/O throughput pose a great challenge on the parallelization. To address this challenge, we propose a general multi-threaded parallel coding approach in this work. The approach consists of a general multi-threaded parallel coding model named as MTPerasure, and two detailed parallel coding algorithms, named as sdaParallel and ddaParallel, respectively, adapting to different I/O circumstances. MTPerasure is a general parallel coding model focusing on the high level data allocation, and it is applicable for all erasure codes and can be implemented without any modifications of the low level coding algorithms. The sdaParallel divides the data into several parts and the data parts are allocated to different threads statically in order to eliminate synchronization latency among multiple threads, which improves the parallel coding performance under the dummy I/O mode. The ddaParallel employs two threads to execute the I/O reading and writing on the basis of small pieces independently, which increases the I/O throughput. Furthermore, the data pieces are assigned to the coding thread dynamically. A special thread scheduling algorithm is also proposed to reduce thread migration latency. To evaluate our proposal, we parallelize the popular open source library jerasure based on our approach. And a detailed performance comparison with the original sequential coding program indicates that the proposed parallel approach outperforms the original sequential program by an extraordinary speedups from 1.4x up to 7x, and achieves better utilization of the computation and I/O resources. key words: erasure codes, storage, parallelism, data allocation, multiple threads, thread affinity, performance evaluation
Construction and Phenotypic Evaluation of aVibrio vulnificus vvpE Mutant for Elastolytic Protease

ABSTRACT Vibrio vulnificus is an opportunistic gram-negative pathogen that commonly contaminates oysters. Predisposed individuals who consume raw oysters can die within days from sepsis, and even otherwise healthy people are susceptible to serious wound infection after contact with contaminated seafood or seawater. Numerous secreted and cell-associated virulence factors have been proposed to account for the fulminating and destructive nature of V. vulnificusinfections. Among the putative virulence factors is an elastolytic metalloprotease. We cloned and sequenced the vvpE gene encoding an elastase of V. vulnificus ATCC 29307. The functions of the elastase were assessed by constructingvvpE insertional knockout mutants and evaluating phenotypic changes in vitro and in mice. Although other types of protease activity were still observed in vvpE mutants, elastase activity was completely absent in the mutants and was restored by reintroducing the recombinant vvpE gene. In contrast to previous characterization of elastase as a potential virulence factor, which was demonstrated by injecting the purified protein into animals, inactivation of the V. vulnificus vvpE gene did not affect the ability of the bacteria to infect mice and cause damage, either locally in subcutaneous tissues or systemically in the liver, in both iron-treated and normal mice. Furthermore, a vvpE mutant was not affected with regard to cytolytic activity toward INT407 epithelial cells or detachment of INT407 cells from culture dishes in vitro. Therefore, it appears that elastase is less important in the pathogenesis of V. vulnificus than would have been predicted by examining the effects of administering purified proteins to animals. However, V. vulnificus utilizes a variety of virulence factors; hence, the effects of inactivation of elastase alone could be masked by other compensatory virulence factors.
An instrument for measuring cancer patients' preferences for support groups.

The purpose of this study was to develop a valid and reliable instrument to assess cancer patients' preferences for all types of social support and organizational features of cancer support groups. The content of the instrument was the result of a detailed analysis of four resources: (1) literature relating to cancer support group interventions, (2) program materials from existing groups, (3) interviews with individuals who developed or directed groups, and (4) interviews with patients who have participated in cancer support groups. A jury of six experts was used to establish content validity of the instrument. The reliability of the instrument was examined by measuring a sample of 258 cancer patients. The reliability coefficients of the instrument were all above . 80, except for two types of social support (instrumental and informational-educational), which were . 72 and . 78, respectively. It was concluded that the instrument produces valid and reliable measurements of cancer patients' preferences for cancer support groups.
A Qualitative Methodology for the Design of Visual Analytic Tools for Emergency Operation Centers

In this article, we present a qualitative methodology for design research in the domain of visual analytics for emergency management. This specific methodology is characterized by using non-participant observation and interviews as methods and by being theoretically informed by the multidisciplinary framework of visual analytics. The methodology collects data about: (1) workflow, (2) informational environment, and (3) information practices and flows. Our main argument is that this methodology addresses a current gap in visual analytics, which lacks user-centered methodological approaches for design and evaluation research.
Analgesic use and pain coping among patients with haemophilia

Summary. Pain is an important consequence of bleeding episodes in haemophilia. Previous research has included pain frequency and severity among measures of illness‐related disability and quality of life in haemophilia, but little systematic evidence exists about analgesic use and pain coping in haemophilia. This paper reports cross‐sectional findings from a national survey of patients with severe haemophilia type A and type B. Respondents provided information about pain frequency, pain coping strategies (using the haemophilia‐adapted CSQ), use of analgesics and other drugs, and described any concerns they had about their drug use. Participants registered with comprehensive care haemophilia centres did not differ from the rest of the sample on any of the factors recorded. Over one‐third of participants expressed concerns about their drug use, the most frequent of which were about dependence on prescribed analgesics. Pain frequency was the most important predictor of analgesic use, but pain coping, and specifically negative thoughts about pain, was associated with concerns about drug use independently of other factors, including analgesic use and pain frequency. Further research will be needed to evaluate possible interventions to promote more effective pain coping in haemophilia, and to examine the possible effects of pain coping on illness outcomes beyond analgesic use, such as well‐being and quality of life.
Molecular Detection of Epiphytic Acaryochloris spp. on Marine Macroalgae

ABSTRACT A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.
Bacterial diversity and active biomass in full-scale granular activated carbon filters operated at low water temperatures

Granular activated carbon (GAC) filtration enhances the removal of natural organic matter and micropollutants in drinking water treatment. Microbial communities in GAC filters contribute to the removal of the biodegradable part of organic matter, and thus help to control microbial regrowth in the distribution system. Our objectives were to investigate bacterial community dynamics, identify the major bacterial groups, and determine the concentration of active bacterial biomass in full-scale GAC filters treating cold (3.7–9.5°C), physicochemically pretreated, and ozonated lake water. Three sampling rounds were conducted to study six GAC filters of different operation times and flow modes in winter, spring, and summer. Total organic carbon results indicated that both the first-step and second-step filters contributed to the removal of organic matter. Length heterogeneity analysis of amplified 16S rRNA genes illustrated that bacterial communities were diverse and considerably stable over time. α-Proteobacteria, β-Proteobacteria, and Nitrospira dominated in all of the GAC filters, although the relative proportion of dominant phylogenetic groups in individual filters differed. The active bacterial biomass accumulation, measured as adenosine triphosphate, was limited due to low temperature, low flux of nutrients, and frequent backwashing. The concentration of active bacterial biomass was not affected by the moderate seasonal temperature variation. In summary, the results provided an insight into the biological component of GAC filtration in cold water temperatures and the operational parameters affecting it. GRAPHICAL ABSTRACT
Gravitational amplitudes in black hole evaporation: the effect of non-commutative geometry

Recent work in the literature has studied the quantum-mechanical decay of a Schwarzschild-like black hole, formed by gravitational collapse, into almost-flat spacetime and weak radiation at a very late time. The relevant quantum amplitudes have been evaluated for bosonic and fermionic fields, showing that no information is lost in collapse to a black hole. On the other hand, recent developments in non-commutative geometry have shown that, in general relativity, the effects of non-commutativity can be taken into account by keeping the standard form of the Einstein tensor on the left-hand side of the field equations and introducing a modified energy–momentum tensor as a source on the right-hand side. The present paper, relying on the recently obtained non-commutativity effect on a static, spherically symmetric metric, considers from a new perspective the quantum amplitudes in black hole evaporation. The general relativity analysis of spin-2 amplitudes is shown to be modified by a multiplicative factor F depending on a constant non-commutativity parameter and on the upper limit R of the radial coordinate. Limiting forms of F are derived which are compatible with the adiabatic approximation here exploited. Approximate formulae for the particle emission rate are also obtained within this framework.
Advances in Brief Enhanced Efficacy of Transcriptionally Targeted Suicide Gene / Prodrug Therapy for Thyroid Carcinoma with the Cre-lox P System

Our recent study demonstrates the feasibility of the thyroglobulin (TG) promoter in transcriptionally targeted gene therapy for thyroid carcinomas expressing TG, albeit less effectively than the constitutive viral promoter. The present study was, therefore, designed to enhance the activity of the TG promoter with the CreloxP system. Our data demonstrate that the in vitro cytotoxic effect of herpes simplex virus thymidine kinase/ ganciclovir obtained with the TG promoter and the Cre-loxP system is ;5–10-fold higher than that with the TG promoter alone. Enhanced tumor growth inhibition was also observed in in vivo tumor models. These data indicate the usefulness of the CreloxP system to enhance the activity of a tissue (or tumor)-specific promoter in transcriptionally targeted cancer gene therapy.
MinCD-independent inhibition of cell division by a protein that fuses MalE to the topological specificity factor MinE

We report that MinE, the topological specificity factor of cell division in Escherichia coli, inhibits septation when fused to the C terminus of the maltose-binding protein MalE. This contrasts with overexpression of MinE alone, which affects growth but has no effect on division. Inhibition by MalE-MinE was minCD independent and depended on MinE segments involved in dimerization and prevention of MinCD division inhibition. The SOS and the heat shock responses were not involved, suggesting that the inhibition comes from a direct interaction of MalE-MinE with the septation apparatus. MalE-MinE lethality was suppressed by overexpression of ftsZ, as well as by overexpression of ftsN, a suppressor of temperature-sensitive mutations in genes ftsQ, ftsA, and ftsI. We also report that high-level synthesis of MalE disturbs nucleoid partitioning.
Preparing secondary computer science teachers through an iterative development process

An enormous challenge to computing education in secondary schools worldwide is the lack of secondary computer science teachers. The Institute for Computing Education (ICE) has been offering teacher professional development in an attempt to increase the quantity and quality of secondary computing teachers in Georgia in the United States of America since 2004. We have developed our teacher professional development over the last ten years with an iterative cycle of design, development, delivery, and evaluation.