Miracle-dz/UniFAR-FTUs huggingface dataset:
Rapid Diagnosis of Human Herpesvirus 6 Infection by a Novel DNA Amplification Method, Loop-Mediated Isothermal Amplification

ABSTRACT A novel nucleic acid amplification method, termed loop-mediated isothermal amplification (LAMP), which amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions, may be a valuable tool for the rapid detection of infectious agents. LAMP was developed for human herpesvirus 6 (HHV-6), and its reliability was evaluated in this study. Although LAMP products were detected in HHV-6 B and HHV-6 A DNA, they were not detected in HHV-7 and human cytomegalovirus DNA. The sensitivity of the original HHV-6 LAMP protocol was 50 copies/tube. In order to increase the method's sensitivity, HHV-6 LAMP was modified by increasing the primer concentration. As a result of the modification, sensitivity increased to 25 copies/tube. After these initial validation studies, 13 patients with fever were tested for HHV-6 by viral isolation, serological analysis, and HHV-6 LAMP. In three of the eight patients with primary HHV-6 infection, HHV-6 DNA was detected in whole blood by the original HHV-6 LAMP protocol in not only the acute phase but also the convalescent phase. HHV-6 DNA was detected by modified HHV-6 LAMP in all eight plasma samples collected in the acute phase; however, no HHV-6 DNA was detected in plasma samples collected in the convalescent phase. Although HHV-6 DNA was detected in both the acute and convalescent phases of whole-blood samples in patients with past HHV-6 infection, it was not detected in plasma samples that did not contain latent viral DNA. Thus, detection of HHV-6 DNA in plasma by using this modified HHV-6 LAMP protocol is appropriate for diagnosis of active HHV-6 infection.
Power System Economic Dispatch Considering Steady-State Secure Region for Wind Power

In this paper, an economic dispatch model considering a flexible generation redispatch is proposed for managing the wind power variability in electric power systems. The model considers the base-case operation cost as well as the steady-state secure region for the variable wind energy. The generation schedule in the secure region provides grid operators with the boundary for absorbing wind power. The proposed model is formulated as a generalized semi-infinite programming problem and the corresponding solution is presented. The impact of flexible thermal resources and transmission capacity on the calculation of the wind power secure region is also discussed. The proposed solution method is analyzed using the modified IEEE-RTS 1979.
Liver Parenchyma Regeneration in Connection with Extended Surgical Procedure - Experiment on Large Animal

Liver surgery underwent enormous evolution after development and introduction of new technical skills in surgical praxis. Nevertheless many patients with primary or secondary liver malignancies are not indicated to radical surgical therapy that could reach complete remission of malignant disease because the frontiers of liver surgery are limited today by the functional reserves of remnant parenchyma. The main argument to non surgical treatment is increased risk of acute liver failure after extended liver resection, where retained liver parenchyma is to small to sustain the liver functions (Abdalla, 2001). Portal vein embolization (PVE) can multiply the future liver remnant volume (FLRV) in spite of affection of only one of liver lobes by malignant diseases (Makuuchi, 1984, Makuuchi, 1990, Harada, 1997). This procedure was performed firstly in 1984 by Makuuchi (Abdalla, 2001, Makuuchi, 1984, Makuuchi, 1990). PVE of portal branch of with malignancy afflicted liver lobe initiates compensatory hypertrophy of contralateral non-occluded lobe. The occluded lobe underlies atrophy. The compensatory hypertrophy is supposed to be stimulated by increased flow of portal blood, that contains hepatotrophic substances (Kusaka, 2004, Azoulay, 2000). Liver resection after PVE is performed only in 63-96% of patients (Kokudo, 200, Stefano, 2005, Lagasse, 2000). The main reason for this resolution is unsuccessful hypertrophy of FLRV or progression of malignancy. Liver resection after PVE is performed only in 63-96% of patients (Azoulay, 200, Kokudo, 2001, Stefano, 2005). The main reason for this resolution are unsuccessful hypertrophy of FLRV or progression of malignancy.
Extraction and characterization of pectin from dragon fruit (Hylocereus polyrhizus) using various extraction conditions. (Pengekstrakan dan pencirian pektin daripada buah naga (hylocereus polyrhizus)menggunakan pelbagai keadaan pengekstrakan)

The extraction of pectin from dragon fruit (Hylocereus polyrhizus) peels under three different extraction conditions was identified as an alternative source of commercial pectin. In this work, dried alcohol-insoluble residues ( AIR) of dragon fruit peels were treated separately with 0.25% ammonium oxalate/oxalic acid at a pH of 4.6 at 85 o C; 0.03 M HCl at a pH of 1.5 at 85 o C; and de-ionized water at 75 o C. The pectin obtained from these methods was compared in terms of yield, physicochemical properties and chemical structure. Fourier Transform Infrared Spectroscopy (FTIR) was used in the identification of dragon fruit pectins. The results showed that the pectin yield (14.96-20.14% based on dry weight), moisture content (11.13-11.33%), ash content (6.88-11.55%), equivalent weight (475.64-713.99), methoxyl content (2.98-4.34%), anhydrouronic acid (45.25-52.45%) and the degree of esterification (31.05-46.96%) varied significantly (p < 0.05) with the various extraction conditions used. Pectin extracted with ammonium oxalate gave the highest yield of pectin, with high purity and low ash content. Based on the value of methoxyl content and the degree of esterification, dragon fruit pectin can be categorized as low-methoxyl pectin.
Coupled-Line Sensor With Marchand Balun as RF System for Dielectric Sample Detection

A novel nondestructive method for effective permittivity measurement and detection of sample's permittivity change in microwave frequency range has been proposed, utilizing a Marchand balun and a coupled-line sensor. The out-of-phase excited coupled-line section is used to obtain high sensitivity on the covering sample, which can be as narrow as the spacing between coupled strips. Formulas describing balanced to unbalanced impedance transformation of a Marchand balun have been provided, hence the proposed method requires only one-port measurement to determine effective permittivity in a wide frequency range. The proposed setup has been theoretically and experimentally investigated. The obtained measurement results proved the usefulness of the proposed approach for dielectric sample detection.
The Statistical Analysis of Circadian Phase and Amplitude in Constant-Routine Core-Temperature Data

Accurate estimation of the phases and amplitude of the endogenous circadian pacemaker from constant-routine core-temperature series is crucial for making inferences about the properties of the human biological clock from data collected under this protocol. This paper presents a set of statistical methods based on a harmonic-regression-plus-correlated-noise model for estimating the phases and the amplitude of the endogenous circadian pacemaker from constant-routine core-temperature data. The methods include a Bayesian Monte Carlo procedure for computing the uncertainty in these circadian functions. We illustrate the techniques with a detailed study of a single subject's core-temperature series and describe their relationship to other statistical methods for circadian data analysis. In our laboratory, these methods have been successfully used to analyze more than 300 constant routines and provide a highly reliable means of extracting phase and amplitude information from core-temperature data.
In vivo quantitative microimaging of rat spinal cord at 7T

In vivo T2, ADC, and MT properties of the GM and WM of the rat spinal cord were measured at 7T in the cervical region. The GM T2, T2GM = 43.2 ± 1.0 msec is significantly reduced compared to the WM T2, T2WM = 57.0 ± 1.6 msec. Diffusion is anisotropic for both GM and WM, with a larger ADC value along the cord axis (ADCGM// = 1.05 ± 0.09 10−9 m2sec−1 and ADCWM// = 1.85 ± 0.18 10−9 m2sec−1) than perpendicular to this plane (ADCGM⊥ ∼ 0.50 * 10−9 m2sec−1 and ADCWM⊥ ∼ 0.18 * 10−9 m2sec−1). The MT properties do not significantly differ between the WM and the GM, but allow one to distinguish the thin CSF layer from the WM. DWI with the sensitizing gradient perpendicular to the cord axis leads to the best contrast between GM and WM in the cervical region. Magn Reson Med 44:893–898, 2000. © 2000 Wiley‐Liss, Inc.
Unanswered questions for management of acute coronary syndrome: risk stratification of patients with minimal disease or normal findings on coronary angiography.

BACKGROUND The prognostic implication of chest pain associated with normal or near-normal findings on angiography is still unknown. We explored outcomes and methods of risk stratification in patients with nonobstructive coronary artery disease in the setting of non-ST-segment elevation acute coronary syndromes. METHODS Data were pooled from 3 Thrombolysis in Myocardial Infarction (TIMI) trials (TIMI 11B, TIMI 16, and TIMI 22). Angiographic data were available on 7656 patients with non-ST-segment elevation acute coronary syndromes. The primary end point of this analysis was the composite of the rates of death, myocardial infarction, unstable angina requiring rehospitalization, revascularization, and stroke at 1-year follow-up. Outcomes were evaluated by mean of the TIMI risk score for developing at least 1 component of the primary end point. RESULTS Angiographic findings showed that 710 (9.1%) of 7656 patients had nonobstructive coronary artery disease; 48.7% of these had normal coronary arteries (0% stenosis), and 51.3% had mild coronary artery disease (>0% to <50% stenosis). A primary end-point event occurred in 101 patients (12.1%). It is noteworthy that a 2% event rate of deaths and myocardial infarctions had occurred in these patients at the 1-year follow-up. Event rates of death and myocardial infarction increased significantly as the TIMI risk score increased from 0.6% for a score of 1 to 4.0% for a score greater than 4. CONCLUSIONS Patients with non-ST-segment elevation acute coronary syndromes with nonobstructive coronary artery disease detected by angiography have a substantial risk of subsequent coronary events within 1 year. The risk is not univariately high, and the TIMI risk score helps to reveal patients at high risk.
A Wireless Location System in LTE Networks

Personal location technologies are becoming important with the rapid development of Mobile Internet services. In traditional cellular networks, the key problems of user location technologies are high-precision synchronization among different base stations, inflexible processing resources, and low accuracy positioning, especially for indoor environment. In this paper, a new LTE location system in Centralized Radio Access Network (C-RAN) is proposed, which makes channel and location measurement more available, allocation of baseband processing resources more flexible, and location service capability opening. The location system contains more than two antenna clusters, and each of them gets time-difference-of-arrival (TDOA) of sounding reference signals (SRSs) from different antennas. Then, based on data provided by location measurement units (LMUs), the location information server calculates TDOAs and derives the users’ position. Furthermore, a new location algorithm is raised which can achieve distributed antennas collaboration and centralized location computing. And an improved optimized algorithm with the best TDOA selection is proposed. Finally, simulations are given out to verify the efficiency of the proposed algorithm in this LTE location system.
A novel index of photoplethysmography by using instantaneous pulse rate variability during non-stationary condition

Heart rate variability (HRV) and pulse rate variability (PRV) have been widely used as an automatic nervous system (ANS) activities observation. Based on the timescale limitation of heart beat (for HRV) and pulse waveform (for PRV), one method of instantaneous pulse rate variability (iPRV) with photoplethysmography (PPG) had been proposed as a HRV and PRV surrogate during non-stationary condition in frequency domain. In this paper, the examination in time-domain PPG during passive leg raise (PLR) trial was studied. The 24 subjects pre-experiment resulted that iPRV and HRV are positive correlation (r-value = 0.680±0.099 in baseline; r-value = 0.688±0.096 in PLR). Furthermore, the iPRV containing much complex intrinsic components on higher frequency bands provides more intrinsic cardiovascular information for further assessment. This proposed examination in time-domain PPG is the possible leading way to more useful indicator for healthcare application.
Pulmonary hypertension as a risk factor for death in patients with sickle cell disease.

1. Caspi D, Lubart E, Graff E, Habot B, Yaron M, Segal R. The effect of mini-dose aspirin on renal function and uric acid handling in elderly patients. Arthritis Rheum 2000;43:103-8. 2. Wallace SL, Robinson H, Masi AT, Decker JL, McCarty DJ, Yu TF. Preliminary criteria for the classification of the acute arthritis of primary gout. Arthritis Rheum 1977;20:895-900. 3. Roubenoff R, Klag MJ, Mead LA, Liang KY, Seidler AJ, Hochberg MC. Incidence and risk factors for gout in white men. JAMA 1991; 266:3004-7. 4. Shadick NA, Kim R, Weiss S, Liang MH, Sparrow D, Hu H. Effect of low level lead exposure on hyperuricemia and gout among middle aged and elderly men: the Normative Aging Study. J Rheumatol 2000;27:1708-12.
Preoperative β‐cell function in patients with type 2 diabetes is important for the outcome of Roux‐en‐Y gastric bypass surgery

Roux‐en‐Y gastric bypass surgery leads to remission of type 2 diabetes in the majority of patients suffering from the disease. The gut hormone glucagon‐like peptide‐1 is believed to be of major importance for the remission process. The present project demonstrates a marked difference in the chance of remission of type 2 diabetes in patients with low or high preoperative β‐cell function in spite of a similar post‐surgery increase in postprandial glucagon‐like peptide‐1 release. Furthermore, post‐surgery intravenous glucose administration, which does not stimulate release of glucagon‐like peptide‐1, leads to increased insulin secretion in the patients with the best preoperative β‐cell function. Together the present findings indicate that patients with type 2 diabetes with high preoperative β‐cell function experience a glucagon‐like peptide‐1‐independent increase in β‐cell function after gastric bypass surgery.
Isolation of IgE from reaginic rat serum.

IgE from the serum of rats immunized with Nippostrongylus brasiliensis was substantially purified. As measured by passive cutaneous anaphylaxis (PCA) activity an overall yield of 45% was reproducibly achieved by a combination of ammonium sulfate precipitation, gel and ion-exchange chromatography, and immunoadsorbents. The iodinated final product was 80% precipitable by specific antisera to IgE, and in radio-immunoelectrophoresis with antiserum to light chains gave only a single line corresponding to the line given by antisera to IgE. No lines could be detected with an antiserum to normal serum proteins devoid of antibodies to light chains. About 80% of the IgE related counts were shown to bind specifically to neoplastic rat basophils. On the basis of these results it was calculated that 18 ng of rat IgE are adequate to give a detectable PCA response.
State estimation of passifiable lurie systems via limited-capacity communication channel

State estimation problems for a class of nonlinear oscillatory systems under information constraints imposed by limited capacity of the communication channel are analyzed. A binary time-varying coder-decoder scheme is described and a theoretical analysis for state estimation for nonlinear plants represented in Lurie form (linear part plus nonlinearity depending only on measurable outputs) based on the Passification Theorem is provided. It is shown that the estimation error exponentially tends to zero for sufficiently high transmission rate (channel capacity). The results are illustrated by example of state estimation of chaotic Chua system via a communication channel with limited capacity.
Tubular fluid concentrations and kidney contents of angiotensins I and II in anesthetized rats.

Previous micropuncture studies have reported nanomolar concentrations of angiotensin II in proximal tubular fluid and have indicated that angiotensin II or a precursor may be secreted into the tubular lumen. Further experiments were performed to determine if proximal tubular fluid angiotensin I concentrations are also greater than plasma and kidney levels and to estimate the degree of intrarenal compartmentalization of the angiotensin peptides. Free-flow proximal tubular fluid samples were collected in micropipets and were pooled for each animal. At the end of each experiment, a blood sample was collected and the micropunctured left kidney was harvested and homogenized in methanol. The angiotensin I concentration in proximal tubular fluid samples averaged 6.1 +/- 1.2 pmol/mL, whereas the angiotensin II concentration averaged 8.1 +/- 1.6 pmol/mL (N = 13). HPLC analysis of a separate sample pooled from collections in five rats indicated that the immunoreactive angiotensin I and angiotensin II primarily represented authentic angiotensin I and II. Plasma concentrations of angiotensin I and angiotensin II averaged 0.39 +/- 0.09 and 0.15 +/- 0.03 pmol/mL, respectively. The kidney contents of angiotensin I and angiotensin II were 1.28 +/- 0.24 and 0.97 +/- 0.17 pmol/g of kidney, respectively. These findings indicate that proximal tubular fluid contains nanomolar concentrations of angiotensin I as well as angiotensin II. These high tubular fluid concentrations, which greatly exceed the plasma and kidney levels, likely reflect net secretion of the angiotensin peptides by proximal tubule cells. (ABSTRACT TRUNCATED AT 250 WORDS)
ulti-parameter microcantilever sensor for comprehensive characterization of ewtonian fluids urali

We have developed a microcantilever-based sensor for the characterization of Newtonian fluids. The vibrating cantilever’s peakfrequency, eigenfrequency and damping due to the surrounding liquid were simultaneously recorded. Various aqueous solutions of glycerol and ethylene glycol with defined densities and viscosities were injected and the cantilever response was tracked in real time. Our experiments demonstrate that only a sensor measuring several parameters simultaneously can discriminate between viscosity and density effects. The sensor is found to be sensitive to liquid properties with a resolution of anomechanical sensor heology icrocantilever esonance amping 1.5% in viscosity and 0.06% in density. The results indicate that the effect of viscosity on the eigenfrequency cannot be neglected even at higher vibrational modes. © 2008 Elsevier B.V. All rights reserved. t q s i p t d e t r a i iscosity ensity ewtonian fluid
Monitoring of the Human Body Signal through the Internet of Things (IoT) Based LoRa Wireless Network System

Internet of Things (IoT) based healthcare system is now at the top peak because of its potentialities among all other IoT applications. Supporting sensors integrated with IoT healthcare can effectively analyze and gather the patients’ physical health data that has made the IoT based healthcare ubiquitously acceptable. A set of challenges including the continuous presence of the healthcare professionals and staff as well as the proper amenities in remote areas during emergency situations need to be addressed for developing a flexible IoT based healthcare system. Besides that, the human entered data are not as reliable as automated generated data. The development of the IoT based health monitoring system allows a personalized treatment in certain circumstances that helps to reduce the healthcare cost and wastage with a continuous improving outcome. We present an IoT based health monitoring system using the MySignals development shield with (Low power long range) LoRa wireless network system. Electrocardiogram (ECG) sensor, body temperature sensor, pulse rate, and oxygen saturation sensor have been used with MySignals and LoRa. Evaluating the performances and effectiveness of the sensors and wireless platform devices are also analyzed in this paper by applying physiological data analysis methodology and statistical analysis. MySignals enables the stated sensors to gather physical data. The aim is to transmit the gathered data from MySignals to a personal computer by implementing a wireless system with LoRa. The results show that MySignals is successfully interfaced with the ECG, temperature, oxygen saturation, and pulse rate sensors. The communication with the hyper-terminal program using LoRa has been implemented and an IoT based healthcare system is being developed in MySignals platform with the expected results getting from the sensors.
An outbreak of chickenpox at a child care centre in Western Australia. Costs to the community and implications for vaccination policy

Objective: Between May and June 2002 an outbreak of chickenpox (CP) occurred at a child care centre in Perth, Western Australia. An epidemiological study was undertaken in order to determine the characteristics of the outbreak, assess vaccine effectiveness, and to define the direct and indirect costs associated with CP infections in young children.
Thermal management of high performance microprocessors in burn-in environment

In deep sub-micron CMOS technologies, increased standby current in high performance processors results in increased junction temperature. This elevated temperature has a positive feedback on the standby current. If the temperature is not controlled, it may lead to thermal runaway. In this paper we investigate the thermal management of high performance chips in the burn-in environment.
Revisiting the management of fatigue in multiple sclerosis in the context of rehabilitation: a narrative review of current evidence

Fatigue is one of the most frequently reported symptoms of multiple sclerosis, interfering with functioning and resulting in activity limitations and participation restrictions. Therefore, it is important to manage fatigue in a rehabilitation context considering not only disability associated with impaired functioning but also all the problems relevant to environmental and personal factors as defined in the International Classification of Functioning, Disability and Health (ICF). In addition to pharmacological treatment, there are many rehabilitation interventions with evidence of effectiveness in reducing fatigue and thereby facilitating improvement, particularly in body functions and structures and activities and participation components of the ICF. However, more research is required to provide evidence for effective treatment strategies in overcoming fatigue and related problems, especially in the components of contextual factors of the ICF.