Miracle-dz/UniFAR-FTUs huggingface dataset:
The transcriptome of retinal Müller glial cells

Müller glial cells are the major type of glia in the mammalian retina. To identify the molecular machinery that defines Müller glial cell identity and function, single cell gene expression profiling was performed on Affymetrix microarrays. Identification of a cluster of genes expressed at high levels suggests a Müller glia core transcriptome, which likely underlies many of the functions of Müller glia. Expression of components of the cell cycle machinery and the Notch pathway, as well as of growth factors, chemokines, and lipoproteins might allow communication between Müller glial cells and the neurons that they support, including modulation of neuronal activity. This approach revealed a set of transcripts that were not previously characterized in (Müller) glia; validation of the expression of some of these genes was performed by in situ hybridization. Genes expressed exclusively by Müller glia were identified as novel markers. In addition, a novel BAC transgenic mouse that expresses Cre in Müller glia cells was generated. The molecular fingerprint of Müller glia provides a foundation for further studies of Müller glia development and function in normal and diseased states. J. Comp. Neurol. 509:225–238, 2008. © 2008 Wiley‐Liss, Inc.
Transferring autonomous reaching and targeting behaviors for cable-driven robots in minimally invasive surgery

Cable-driven mechanisms have been widely used as compliant actuators in surgical robots over last decades due to their superior performance of dexterous operation in confined workspace. And minimally invasive surgery (MIS) is one of the most important applications of such systems. In MIS, point to point reaching and targeting behavior is the most fundamental movement primitive, typical examples include leading the robot tool to the target lesions. Currently, the motion control of such surgical robots in MIS are realized by clinical staff with haptic devices. However, due to the totally different configurations of the robot and the haptic device, and the friction loss, viscoelasticity, hysteresis and nonstationary behaviors inherently in the cable-driven mechanisms, the teleoperation procedure is difficult and uncomfortable, and may cause severe fatigues of the surgeons. In this work, a novel motion control approach, learning from demonstration (LfD), is used to transfer autonomous reaching and targeting skills from human experts to a cable-driven surgical robot. A modulated first order dynamical systems model is used to encode human demonstrations and generate executable paths for the robot. Experiments have been performed on a seven degrees-of-freedom KUKA LBR robot and a three degrees-of-freedom tendon-driven serpentine manipulator (TSM) to validate the proposed methods.
The Effects of Ingestion of Sugarcane Juice and Commercial Sports Drinks on Cycling Performance of Athletes in Comparison to Plain Water

Purpose Sugarcane juice (ScJ) is a natural drink popular in most tropical Asian regions. However, research on its effect in enhancing sports performance is limited. The present investigation was to study the effect of sugarcane juice on exercise metabolism and sport performance of athletes in comparison to a commercially available sports drinks. Methods Fifteen male athletes (18-25 yrs) were asked to cycle until volitional exhaustion at 70% VO2 max on three different trials viz. plain water (PW), sports drink (SpD) and ScJ. In each trial 3ml/kg/BW of 6 % of carbohydrate (CHO) fluid was given at every 20 min interval of exercise and a blood sample was taken to measure the hematological parameters. During recovery 200 ml of 9% CHO fluid was given and blood sample was drawn at 5, 10, 15 min of recovery. Results Ingestion of sugarcane juice showed significant increase (P<0.05) in blood glucose levels during and after exercise compared to SpD and PW. However, no significant difference was found between PW, SpD and ScJ for total exercise time, heart rate, blood lactate and plasma volume. Conclusion ScJ may be equally effective as SpD and PW during exercise in a comfortable environment (<30°C) and a more effective rehydration drink than SpD and PW in post exercise as it enhances muscle glycogen resynthesis.
Interactive Teaching Strategies for Agent Training

Agents learning how to act in new environments can benefit from input from more experienced agents or humans. This paper studies interactive teaching strategies for identifying when a student can benefit from teacher-advice in a reinforcement learning framework. In student-teacher learning, a teacher agent can advise the student on which action to take. Prior work has considered heuristics for the teacher to choose advising opportunities. While these approaches effectively accelerate agent training, they assume that the teacher constantly monitors the student. This assumption may not be satisfied with human teachers, as people incur cognitive costs of monitoring and might not always pay attention. We propose strategies for a teacher and a student to jointly identify advising opportunities so that the teacher is not required to constantly monitor the student. Experimental results show that these approaches reduce the amount of attention required from the teacher compared to teacher-initiated strategies, while maintaining similar learning gains. The empirical evaluation also investigates the effect of the information communicated to the teacher and the quality of the student's initial policy on teaching outcomes.
Exendin-4 Suppresses Src Activation and Reactive Oxygen Species Production in Diabetic Goto-Kakizaki Rat Islets in an Epac-Dependent Manner

OBJECTIVE Reactive oxygen species (ROS) is one of most important factors in impaired metabolism secretion coupling in pancreatic β-cells. We recently reported that elevated ROS production and impaired ATP production at high glucose in diabetic Goto-Kakizaki (GK) rat islets are effectively ameliorated by Src inhibition, suggesting that Src activity is upregulated. In the present study, we investigated whether the glucagon-like peptide-1 signal regulates Src activity and ameliorates endogenous ROS production and ATP production in GK islets using exendin-4. RESEARCH DESIGN AND METHODS Isolated islets from GK and control Wistar rats were used for immunoblotting analyses and measurements of ROS production and ATP content. Src activity was examined by immunoprecipitation of islet lysates followed by immunoblotting. ROS production was measured with a fluorescent probe using dispersed islet cells. RESULTS Exendin-4 significantly decreased phosphorylation of Src Tyr416, which indicates Src activation, in GK islets under 16.7 mmol/l glucose exposure. Glucose-induced ROS production (16.7 mmol/l) in GK islet cells was significantly decreased by coexposure of exendin-4 as well as PP2, a Src inhibitor. The Src kinase–negative mutant expression in GK islets significantly decreased ROS production induced by high glucose. Exendin-4, as well as PP2, significantly increased impaired ATP elevation by high glucose in GK islets. The decrease in ROS production by exendin-4 was not affected by H-89, a PKA inhibitor, and an Epac-specific cAMP analog (8CPT-2Me-cAMP) significantly decreased Src Tyr416 phosphorylation and ROS production. CONCLUSIONS Exendin-4 decreases endogenous ROS production and increases ATP production in diabetic GK rat islets through suppression of Src activation, dependently on Epac.
Dietary phosphatidylcholine and risk of all-cause and cardiovascular-specific mortality among US women and men.

BACKGROUND The trimethylamine-containing nutrient phosphatidylcholine is the major dietary source for the gut microbiota metabolite trimethylamine-N-oxide (TMAO), which has been related to cardiovascular diseases (CVDs) and mortality. Previous research suggested that the relation of TMAO with CVD risk might be stronger in diabetic than in nondiabetic populations. However, the evidence for an association of dietary phosphatidylcholine with CVD and mortality is limited. OBJECTIVES We aimed to examine whether dietary consumption of phosphatidylcholine, which is mainly derived from eggs, red meat, and fish, is related to all-cause and CVD mortality in 2 cohorts of US women and men. In particular, we also tested if such an association was modified by diabetes status. DESIGN We followed 80,978 women from the Nurses' Health Study (1980-2012) and 39,434 men from the Health Professionals Follow-Up Study (1986-2012), who were free of cancer and CVD at baseline, for mortality. Dietary intakes and potential confounders were assessed with regularly administered questionnaires. We used Cox proportional hazards models to estimate HRs and 95% CIs. RESULTS We documented 17,829 all-cause and 4359 CVD deaths during follow-up. After multivariate adjustment for potential confounders, including demographic factors, disease status, lifestyle, and dietary intakes, higher phosphatidylcholine intakes were associated with an increased risk of all-cause and CVD mortality. HRs (95% CIs) comparing the top and bottom quintiles of phosphatidylcholine intake were 1.11 (1.06, 1.17; P-trend across quintiles < 0.0001) for all-cause mortality and 1.26 (1.15, 1.39; P-trend < 0.0001) for CVD mortality in the combined data of both cohorts. The associations of phosphatidylcholine with all-cause and CVD mortality were stronger in diabetic than in nondiabetic participants (P-interaction = 0.0002 and 0.001, respectively). CONCLUSION These data suggest that higher phosphatidylcholine consumption is associated with increased all-cause and CVD mortality in the US population, especially in patients with diabetes, independent of traditional risk factors.
Quantum-state engineering with Josephson-junction devices

Quantum-state engineering, i.e., active control over the coherent dynamics of suitable quantum-mechanical systems, has become a fascinating prospect of modern physics. With concepts developed in atomic and molecular physics and in the context of NMR, the field has been stimulated further by the perspectives of quantum computation and communication. Low-capacitance Josephson tunneling junctions offer a promising way to realize quantum bits (qubits) for quantum information processing. The article reviews the properties of these devices and the practical and fundamental obstacles to their use. Two kinds of device have been proposed, based on either charge or phase (flux) degrees of freedom. Single- and two-qubit quantum manipulations can be controlled by gate voltages in one case and by magnetic fields in the other case. Both kinds of device can be fabricated with present technology. In flux qubit devices, an important milestone, the observation of superpositions of different flux states in the system eigenstates, has been achieved. The Josephson charge qubit has even demonstrated coherent superpositions of states readable in the time domain. There are two major problems that must be solved before these devices can be used for quantum information processing. One must have a long phase coherence time, which requires that external sources of dephasing be minimized. The review discusses relevant parameters and provides estimates of the decoherence time. Another problem is in the readout of the final state of the system. This issue is illustrated with a possible realization by a single-electron transistor capacitively coupled to the Josephson device, but general properties of measuring devices are also discussed. Finally, the review describes how the basic physical manipulations on an ideal device can be combined to perform useful operations.
Structural and functional studies of ferredoxin and oxygenase components of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2

3-nitrotoluene dioxygenase (3NTDO) from Diaphorobacter sp. strain DS2 catalyses the conversion of 3-nitrotoluene (3NT) into a mixture of 3- and 4-methylcatechols with release of nitrite. We report here, X-ray crystal structures of oxygenase and ferredoxin components of 3NTDO at 2.9 Å and 2.4 Å, respectively. The residues responsible for nitrite release in 3NTDO were further probed by four single and two double mutations in the catalytic site of α-subunit of the dioxygenase. Modification of Val 350 to Phe, Ile 204 to Ala, and Asn258 to Val by site directed mutagenesis resulted in inactive enzymes revealing the importance of these residues in catalysis. Docking studies of meta nitrotoluene to the active site of 3NTDO suggested possible orientations of binding that favor the formation of 3-methylcatechol (3MC) over 4-methylcatechol energetically. The electron transfer pathway from ferredoxin subunit to the active site of the oxygenase subunit is also proposed.
Efficacy of Long-Term 4.0 g/Day Mesalazine (Pentasa) for Maintenance Therapy in Ulcerative Colitis

Background High-dose (4.0 g/day) mesalazine is typically used for induction therapy, but its efficacy as maintenance therapy remains to be determined. We conducted a multicenter retrospective study to investigate the efficacy of continuous treatment with 4.0 g/day of mesalazine. Material/Methods Japanese ulcerative colitis (UC) patients receiving acute induction therapy with 4.0 g/day mesalazine were enrolled and followed. Those who clinically improved or who achieved clinical remission were categorized into 2 sub-groups according to the median duration of treatment with 4.0 g/day of mesalazine. The clinical relapse frequency and the time to relapse were analyzed. Results We enrolled 180 patients with active UC, and then 115 patients who clinically improved or who achieved clinical remission after treatment with 4.0 g/day mesalazine were categorized into 2 sub-groups according to the median of treatment duration: a short-term treatment group (≤105 days, n=58) and a long-term treatment group (>105 days, n=57). Overall, 45 (39.1%) patients relapsed: 28 (48.3%) in the short-term treatment group and 17 (29.8%) in the long-term treatment group. This difference was statistically significant (p<0.05). The relapse-free rate in the long-term treatment group was significantly higher than that in the short-term treatment group (p<0.05). The mean time to relapse in the long-term treatment group was significantly longer than that in the short-term treatment group (425.6±243.8 days vs. 277.4±224.5 days; p<0.05). Conclusions Long-term continuous treatment with high-dose mesalazine (4.0 g/day) may be more effective than short-term treatment for maintenance of remission in UC patients.
Probe Sensor Using Nanostructured Multi-Walled Carbon Nanotube Yarn for Selective and Sensitive Detection of Dopamine

The demands for electrochemical sensor materials with high strength and durability in physiological conditions continue to grow and novel approaches are being enabled by the advent of new electromaterials and novel fabrication technologies. Herein, we demonstrate a probe-style electrochemical sensor using highly flexible and conductive multi-walled carbon nanotubes (MWNT) yarns. The MWNT yarn-based sensors can be fabricated onto micro Pt-wire with a controlled diameter varying from 100 to 300 µm, and then further modified with Nafion via a dip-coating approach. The fabricated micro-sized sensors were characterized by electron microscopy, Raman, FTIR, electrical, and electrochemical measurements. For the first time, the MWNT/Nafion yarn-based probe sensors have been assembled and assessed for high-performance dopamine sensing, showing a significant improvement in both sensitivity and selectivity in dopamine detection in presence of ascorbic acid and uric acid. It offers the potential to be further developed as implantable probe sensors.
The Effect of Performance-Based Incentive Contracts on System 1 and System 2 Processing in Affective Decision Contexts: fMRI and Behavioral Evidence

ABSTRACT: Managers may rely on emotional reactions to a setting to the detriment of economic considerations (“System 1 processing”), resulting in decisions that are costly for firms. While economic theory prescribes performance-based incentives to align goals and induce effort, psychology theory suggests that the salience of emotions is difficult to overcome without also inducing more deliberate consideration of both emotional and economic factors (“System 2 processing”). We link these perspectives by investigating whether performance-based incentives mitigate the costly influence of emotion by inducing more System 2 processing. Using functional magnetic resonance imaging and traditional experiments, we investigate managers' brain activity and choices under fixed wage and performance-based contracts. Under both, brain regions associated with System 1 processing are more active when emotion is present. Relative to fixed wage contracts, performance-based contracts induce System 2 processing in emotional co.
Characterization of a rice variety with high hydraulic conductance and identification of the chromosome region responsible using chromosome segment substitution lines.

BACKGROUND AND AIMS The rate of photosynthesis in paddy rice often decreases at noon on sunny days because of water stress, even under submerged conditions. Maintenance of higher rates of photosynthesis during the day might improve both yield and dry matter production in paddy rice. A high-yielding indica variety, 'Habataki', maintains a high rate of leaf photosynthesis during the daytime because of the higher hydraulic conductance from roots to leaves than in the standard japonica variety 'Sasanishiki'. This research was conducted to characterize the trait responsible for the higher hydraulic conductance in 'Habataki' and identified a chromosome region for the high hydraulic conductance. METHODS Hydraulic conductance to passive water transport and to osmotic water transport was determined for plants under intense transpiration and for plants without transpiration, respectively. The varietal difference in hydraulic conductance was examined with respect to root surface area and hydraulic conductivity (hydraulic conductance per root surface area, L(p)). To identify the chromosome region responsible for higher hydraulic conductance, chromosome segment substitution lines (CSSLs) derived from a cross between 'Sasanishiki' and 'Habataki' were used. KEY RESULTS The significantly higher hydraulic conductance resulted from the larger root surface area not from L(p) in 'Habataki'. A chromosome region associated with the elevated hydraulic conductance was detected between RM3916 and RM2431 on the long arm of chromosome 4. The CSSL, in which this region was substituted with the 'Habataki' chromosome segment in the 'Sasanishiki' background, had a larger root mass than 'Sasanishiki'. CONCLUSIONS The trait for increasing plant hydraulic conductance and, therefore, maintaining the higher rate of leaf photosynthesis under the conditions of intense transpiration in 'Habataki' was identified, and it was estimated that there is at least one chromosome region for the trait located on chromosome 4.
Inhibitory Control and L2 Proficiency Modulate Bilingual Language Production: Evidence from Spontaneous Monologue and Dialogue Speech

Bilingual language production requires that speakers recruit inhibitory control (IC) to optimally balance the activation of more than one linguistic system when they produce speech. Moreover, the amount of IC necessary to maintain an optimal balance is likely to vary across individuals as a function of second language (L2) proficiency and inhibitory capacity, as well as the demands of a particular communicative situation. Here, we investigate how these factors relate to bilingual language production across monologue and dialogue spontaneous speech. In these tasks, 42 English–French and French–English bilinguals produced spontaneous speech in their first language (L1) and their L2, with and without a conversational partner. Participants also completed a separate battery that assessed L2 proficiency and inhibitory capacity. The results showed that L2 vs. L1 production was generally more effortful, as was dialogue vs. monologue speech production although the clarity of what was produced was higher for dialogues vs. monologues. As well, language production effort significantly varied as a function of individual differences in L2 proficiency and inhibitory capacity. Taken together, the overall pattern of findings suggests that both increased L2 proficiency and inhibitory capacity relate to efficient language production during spontaneous monologue and dialogue speech.
Automated Audio Data Monitoring for a Social Robot in Ambient Assisted Living Environments

Human life expectancy has steadily grown over the last century, which has driven governments and institutions to increase the efforts on caring about the eldest segment of the population. Although this concern was initially addressed by building larger hospitals and retirement homes, these facilities have been rapidly overfilled and their associated maintenance costs are becoming far prohibitive. Therefore, modern trends attempt to take advantage of latest advances in technology and communications to remotely monitor those people with special needs at their own home, which boosts their life quality and has very few impact on their social lives. Nonetheless, this approach still requires a considerable amount of qualified medical personnel to track every patient at any time. The purpose of this paper is to present a social robot for assisted living that tracks patients status by automatically identifying and analyzing the acoustic events happening in a house. Specifically, we have taken benefit of the amazing capabilities of a Raspberry Pi together with a Nao robot to collect data inside a house and send it in realtime to the medical center. Conducted experiments verify the feasibility of our approach and open new research directions in this domain. Keywords— Assisted living, human robot interaction, social robots, remote therapy, audio recognition
Short-Range Millimeter-Wave Radar Perception in a Polar Environment

Autonomous vehicle operations in Antarctica challenge robotic perception. Flying ice and snow, changing illumination due to low sun angles and lack of contrast degrade stereo and laser sensing. Millimeter-wave radar offers remarkable advantages as a robotic perception modality because it is not as sensitive to the aforementioned conditions. Experiments with millimeter-wave radar in an Antarctic environment show minimal degradation of millimeter-wave sensing capabilities under blowing-snow conditions, as well as backscatter obtained from polar-terrain surfaces at grazing angles and detection of obstacles commonly found in polar areas. This paper presents issues relevant to short-range radar perception for a mobile robot in an Antarctic environment. The article describes the experiments and data-analysis procedures, and draws conclusions on the utility of millimeter-wave radar as a robotic sensor for obstacle avoidance and navigation in polar settings. Keywords–Millimeter-wave radar, millimeter-wave imaging, radar scattering, mobile robots, robot sensing.