Miracle-dz/UniFAR-FTUs huggingface dataset:
Allelic variance between GRM6 mutants, Grm6nob3 and Grm6nob4 results in differences in retinal ganglion cell visual responses

An electroretinogram (ERG) screen identified a mouse with a normal a‐wave but lacking a b‐wave, and as such it was designated no b‐wave3 (nob3). The nob3 phenotype mapped to chromosome 11 in a region containing the metabotropic glutamate receptor 6 gene (Grm6). Sequence analyses of cDNA identified a splicing error in Grm6, introducing an insertion and an early stop codon into the mRNA of affected mice (designated Grm6nob3). Immunohistochemistry of the Grm6nob3 retina showed that GRM6 was absent. The ERG and visual behaviour abnormalities of Grm6nob3 mice are similar to Grm6nob4 animals, and similar deficits were seen in compound heterozygotes (Grm6nob4/nob3), indicating that Grm6nob3 is allelic to Grm6nob4. Visual responses of Grm6nob3 retinal ganglion cells (RGCs) to light onset were abnormal. Grm6nob3 ON RGCs were rarely recorded, but when they were, had ill‐defined receptive field (RF) centres and delayed onset latencies. When Grm6nob3 OFF‐centre RGC responses were evoked by full‐field stimulation, significantly fewer converted that response to OFF/ON compared to Grm6nob4 RGCs. Grm6nob4/nob3 RGC responses verified the conclusion that the two mutants are allelic. We propose that Grm6nob3 is a new model of human autosomal recessive congenital stationary night blindness. However, an allelic difference between Grm6nob3 and Grm6nob4 creates a disparity in inner retinal processing. Because the localization of GRM6 is limited to bipolar cells in the On pathway, the observed difference between RGCs in these mutants is likely to arise from differences in their inputs.
An integrin-α4–14-3-3ζ–paxillin ternary complex mediates localised Cdc42 activity and accelerates cell migration

α4 integrins are used by leukocytes and neural crest derivatives for adhesion and migration during embryogenesis, immune responses and tumour invasion. The pro-migratory activity of α4 integrin is mediated in part through the direct binding of the cytoplasmic domain to paxillin. Here, using intermolecular FRET and biochemical analyses, we report a novel interaction of the α4 integrin cytoplasmic domain with 14-3-3ζ. This interaction depends on serine phosphorylation of α4 integrin at a site (S978) distinct from that which regulates paxillin binding (S988). Using a combination of metabolic labelling and targeted mass spectrometry by multiple reaction monitoring we demonstrate the low stoichiometry phosphorylation of S978. The interaction between α4 integrin and 14-3-3ζ is enhanced by the direct association between 14-3-3ζ and paxillin, resulting in the formation of a ternary complex that stabilises the recruitment of each component. Although pair-wise interaction between α4 integrin and paxillin is sufficient for normal Rac1 regulation, the integrity of the ternary complex is essential for focused Cdc42 activity at the lamellipodial leading edge and directed cell movement. Taken together, these data identify a key signalling nexus mediating α4 integrin-dependent migration.
Long-Term Follow-Up of Methicillin-Resistant Staphylococcus aureus Molecular Epidemiology after Emergence of Clone USA300 in San Francisco Jail Populations

ABSTRACT We performed a longitudinal analysis of 502 unique methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates originating from San Francisco jail inmates between 2000 and 2007. Strain USA300, first encountered in 2001, accounted for 82.1% (412/502) of MRSA infections. Non-USA300 MRSA strains were rarely found after 2005 (one isolate in 2006, three in 2007).
Estimating the number of HIV-infected injection drug users in Bangkok: a capture--recapture method.

OBJECTIVES The purpose of the study was to estimate the number of injection drug users infected with the human immunodeficiency virus (HIV) in Bangkok to allow planning for health services for this population. METHODS A two-sample capture-recapture method was used. The first capture listed all persons on methadone treatment for opiate addiction from April 17 through May 17, 1991, at 18 facilities in Bangkok. The second capture involved urine testing of persons held at 72 Bangkok police stations from June 3 through September 30, 1991. Persons whose urine tests were positive for opiate metabolites or methadone were included on the second list. RESULTS The first capture comprised 4064 persons and the recapture 1540 persons. There were 171 persons included on both lists, yielding an estimate of 36,600 opiate users in Bangkok. Existing data indicate that 89% of opiate users in Bangkok inject drugs and that about one third are infected with HIV, yielding an estimate of approximately 12,000 HIV-infected injection drug users in Bangkok in 1991. CONCLUSIONS During the 1990s the number of cases of acquired immunodeficiency syndrome (AIDS) and other HIV-related diseases, including tuberculosis, in the population of HIV-infected injection drug users in Bangkok will increase dramatically, placing new demands on existing health care facilities. The capture-recapture method may be useful in estimating difficult-to-count populations, including injection drug users.
System design and experimental results of polarimetric SAR interferometry

Polarimetric SAR interferometry (POLINSAR) is a very promising technique by combining polarimetric technique and interferometric technique. It can be applied to the area of remote sensing and measurement of structure over forest terrain. At present, many POLINSAR systems were developed, and the first POLINSAR prototype in China is also developed by 38th Research Institute of CETC. Lots of data are acquired by these systems, so POLINSAR techniques have been studied as hot research topics in the area of SAR remote sensing in recent years. In this paper, we introduce design of the POLINSAR prototype and some experimental results. This system can obtain 2m elevation accuracy by data processing.
Renewable energy, rents and GDP growth in MENA countries

ABSTRACT This is an empirical study on the causal relationship between economic growth and renewable energy for MENA countries in a multivariate panel framework over the period 1997–2009 using a fixed effects model with time effects and including combustible waste, employment, fossil fuel consumption, rents and political stability as additional independent variables in the model. Results show that renewable energy affects GDP growth negatively in the long run while there is a short-run bidirectional relationship between renewable energy and fossil fuel energy consumption. In the long run, GDP is affected by rents and political stability. Also, the dynamic effects model provides evidence that for Iraq, there are additional parameters that affect the renewable energy–growth relationship.
Understanding coping strategies among people living with scleroderma: a focus group study

Abstract Purpose: Systemic sclerosis or scleroderma is a chronic, rare connective tissue disease with negative physical and psychological implications. Coping strategies used by scleroderma patients have not been studied in-depth. The objective of the present study was to gain a greater understanding of the coping strategies employed by people living with scleroderma. Method: Three semi-structured focus group discussions were conducted with a total of 22 people with scleroderma. Interviews were recorded, transcribed, and analyzed using content analysis. Coping strategies discussed were analyzed through Lazarus and Folkman’s theoretical model of coping, including: (1) problem-focused, (2) emotion-focused, and (3) meaning-focused coping. Results: Participants reported using a combination of problem-focused (e.g., professional help; seeking disease-related information), emotion-focused (e.g., social support; adaptive distraction techniques), and meaning-focused coping strategies (e.g., benefit finding; goal reappraisal) to help them to cope with and manage their disease. However, many patients reported having difficulty in accessing support services. Conclusions: Scleroderma patients use similar coping strategies as patients with more common diseases, but they may not have access to the same level of support services. Accessible interventions, including self-management programs, aimed at improving problem- and emotion-focused coping are needed. Further, increased access to support groups may provide patients with opportunities to obtain social support and enhance coping.
Optimal Portfolios of Illiquid Assets

This paper investigates the investment behaviour of a large unregulated financial institution (FI) with CARA risk preferences. It shows how the FI optimizes its trading to account for market illiquidity using an extension of the Almgren-Chriss market impact model of multiple risky assets. This expected utility optimization problem over the set of adapted strategies turns out to have the same solutions as a mean-variance optimization over deterministic trading strategies. That means the optimal adapted trading strategy is both deterministic and time-consistent. It is also found to have an explicit closed form that clearly displays interesting properties. For example, the classic constant Merton portfolio strategy, a particular solution of the frictionless limit of the problem, behaves like an attractor in the space of more general solutions. The main effect of temporary market impact is to slow down the speed of convergence to this constant Merton portfolio. The effect of permanent market impact is to incentivize the FI to buy additional risky assets near the end of the period. This property, that we name the Ponzi property, is related to the creation and bursting of bubbles in the market. The proposed model can be used as a stylized dynamic model of a typical FI in the study of the asset fire sale channel relevant to understanding systemic risk and financial stability.
Weather-related effects on woodland vernal pool hydrology and hydroperiod

Woodland vernal pools occur commonly throughout northeastern North America. These pools provide preferred breeding habitat for mole salamanders (Ambystoma spp.) and wood frogs (Rana sylvatica) and support an abundant and diverse macroinvertebrate fauna. Vernal pool hydrology, and especially hydroperiod or duration of the wet phase, affects the composition and productivity of pool fauna. The hydrology of ephemeral wetlands is dominated by local weather conditions. In this paper, I report a ten-year record of the relationships between precipitation and evapotranspiration and water-level change and hydroperiod in four typical southern New England vernal pools. Long-term average precipitation is evenly distributed throughout the year in the Northeast; potential evapotranspiration peaks in the summer months and exceeds precipitation from mid-June through mid-September. This period of water deficit causes the period of maximum vernal pool drying. Vernal pool hydroperiods were shorter and pools dried earlier in those years with larger cumulative water deficits, especially when early spring ground-water resources were below long-term means and late winter snowpack was reduced or absent. Weekly water-level change in vernal pools was significantly related to precipitation and potential evapotranspiration, with precipitation having 2–5 times greater effect than evapotranspiration. Under elimate-change predictions of more episodic precipitation and increased evapotranspiration, vernal pools would dry earlier in the year and remain dry longer. These changes would adversely affect the successful reproduction of pool-breeding amphibians and isolate the remaining productive pools.
Implementation of Screening, Brief Intervention, and Referral to Treatment for Adolescents in Pediatric Primary Care: A Cluster Randomized Trial.

IMPORTANCE Early intervention for substance use is critical to improving adolescent outcomes. Studies have found promising results for Screening, Brief Intervention, and Referral to Treatment (SBIRT), but little research has examined implementation. OBJECTIVE To compare SBIRT implementation in pediatric primary care among trained pediatricians, pediatricians working in coordination with embedded behavioral health care practitioners (BHCPs), and usual care (UC). DESIGN, SETTING, AND PARTICIPANTS The study is a 2-year (November 1, 2011, through October 31, 2013), nonblinded, cluster randomized, hybrid implementation and effectiveness trial examining SBIRT implementation outcomes across 2 modalities of implementation and UC. Fifty-two pediatricians from a large general pediatrics clinic in an integrated health care system were randomized to 1 of 3 SBIRT implementation arms; patients aged 12 to 18 years were eligible. INTERVENTIONS Two modes of SBIRT implementation, (1) pediatrician only (pediatricians trained to provide SBIRT) and (2) embedded BHCP (BHCP trained to provide SBIRT), and (3) UC. MAIN OUTCOMES AND MEASURES Implementation of SBIRT (primary outcome), which included assessments, brief interventions, and referrals to specialty substance use and mental health treatment. RESULTS The final sample included 1871 eligible patients among 47 pediatricians; health care professional characteristics did not differ across study arms. Patients in the pediatrician-only (adjusted odds ratio [AOR], 10.37; 95% CI, 5.45-19.74; P < .001) and the embedded BHCP (AOR, 18.09; 95% CI, 9.69-33.77; P < .001) arms had higher odds of receiving brief interventions compared with patients in the UC arm. Patients in the embedded BHCP arm were more likely to receive brief interventions compared with those in the pediatrician-only arm (AOR, 1.74; 95% CI, 1.31-2.31; P < .001). The embedded BHCP arm had lower odds of receiving a referral compared with the pediatrician-only (AOR, 0.58; 95% CI, 0.43-0.78; P < .001) and UC (AOR, 0.65; 95% CI, 0.48-0.89; P = .006) arms; odds of referrals did not differ between the pediatrician-only and UC arms. CONCLUSIONS AND RELEVANCE The intervention arms had better screening, assessment, and brief intervention rates than the UC arm. Patients in the pediatrician-only and UC arms had higher odds of being referred to specialty treatment than those in the embedded BHCP arm, suggesting lingering barriers to having pediatricians fully address substance use in primary care. Findings also highlight age and ethnic groups less likely to receive these important services. TRIAL REGISTRATION Clinicaltrials.gov Identifier: NCT02408952.
Random Forest: A Classification and Regression Tool for Compound Classification and QSAR Modeling

A new classification and regression tool, Random Forest, is introduced and investigated for predicting a compound's quantitative or categorical biological activity based on a quantitative description of the compound's molecular structure. Random Forest is an ensemble of unpruned classification or regression trees created by using bootstrap samples of the training data and random feature selection in tree induction. Prediction is made by aggregating (majority vote or averaging) the predictions of the ensemble. We built predictive models for six cheminformatics data sets. Our analysis demonstrates that Random Forest is a powerful tool capable of delivering performance that is among the most accurate methods to date. We also present three additional features of Random Forest: built-in performance assessment, a measure of relative importance of descriptors, and a measure of compound similarity that is weighted by the relative importance of descriptors. It is the combination of relatively high prediction accuracy and its collection of desired features that makes Random Forest uniquely suited for modeling in cheminformatics.
Nitric oxide synthase inhibition attenuates cutaneous vasodilation during postmenopausal hot flash episodes

Objective: The purpose of this study was to test the hypothesis that local inhibition of nitric oxide and prostaglandin synthesis attenuates cutaneous vasodilator responses during postmenopausal hot flash episodes. Methods: Four microdialysis membranes were inserted into the forearm skin (dorsal surface) of eight postmenopausal women (mean ± SD age, 51 ± 7 y). Ringer solution (control), 10 mM ketorolac (Keto) to inhibit prostaglandin synthesis, 10 mM NG-l-arginine methyl ester (l-NAME) to inhibit nitric oxide synthase, and a combination of 10 mM Keto + 10 mM l-NAME were each infused at the separate sites. Skin blood flow at each site was indexed using laser-Doppler flowmetry. Cutaneous vascular conductance (CVC) was calculated as laser-Doppler flux/mean arterial blood pressure and was expressed as a percentage of the maximal calculated CVC (CVCmax) obtained after infusion of 50 mM sodium nitroprusside at all sites at the end of the study. Data from 13 hot flash episodes were analyzed. Results: At the control site, the mean ± SD peak increase in CVC was 15.5% ± 6% CVCmax units. This value was not different relative to the peak increase in CVC at the Keto site (13.0% ± 5% CVCmax units; P = 0.09). However, the peak increase in CVC during hot flash episodes were attenuated at the l-NAME and l-NAME + Keto sites (7.4% ± 4% and 8.7% ± 7% CVCmax units, respectively) relative to both the control and the Keto sites (P < 0.05 for both comparisons). There were no significant differences in the peak increases in sweat rate between any of the sites (P = 0.24). Conclusions: These data demonstrate that the mechanism for cutaneous vasodilation during hot flash episodes has a nitric oxide component. Increases in CVC despite the inhibition of prostaglandin synthesis suggest that prostaglandins do not contribute to cutaneous vasodilation during hot flash episodes.
The development of a questionnaire for assessing teachers’ beliefs about science and science teaching in Taiwan and Australia

This paper reports on part of a cross-national study of science classroom environments in Taiwan and Australia. It focuses on teachers' beliefs about science and science teaching and the effects these beliefs have on the learning environment in the science classroom. Better understanding of this relationship between teacher beliefs and learning environment can lead to the identification of the barriers to the introduction of constructivist teaching approaches in school science. Descriptions of the Classroom Learning Environment Survey and the Beliefs about Science and School Science Questionnaire are provided. Statistical analyses of questionnaire data for each country are also provided and contain internal consistency reliability, discriminant validity, mean scores, and standard deviations. Recommendations for further research include using existing questionnaires as interview/observation frameworks and for future research to be guided by a set of interpretive research questions.
Quality dimensions of Delone-McLean model to measure students' accounting computer satisfaction: An empirical test on accounting system information

Accounting software development has become a high-growth industry. User satisfaction is the most widely measure used to assess information system success in Delone-McLean model. This research aims to analyze any factors measuring on the success on utilization of accounting information system by using Delone & McLean information system success model on Zahir Accounting accounting information system. This accounting information system is an accounting information system used by 172 respondents namely students in department of computer accounting in the learning of Computer Accounting Application subject. Results of the research show that the quality of accounting information utilization greatly affects on the net benefit perceived by the respondents. It means that students in department of computer accounting are satisfied to this accounting information system and provide effects on the creativity, increase users' knowledge, convenience in using the system, ability for problem solving, increase students' participation and they are willing to share experiences in using the accounting information system that they use. For the value of Goodness Of Fit (GOF), it is obtained by 0,74 or 74%, it means that these research results have been well-adjusted substantially to represent real condition perceived by users.
Pdx1 regulates pancreas tubulogenesis and E-cadherin expression

Current efforts in developing treatments for diabetes focus on in vitro generation of functional β-cells for cell replacement therapies; however, these attempts have only been partly successful because factors involved in islet formation remain incompletely understood. The embryonic pancreas, which gives rise to β-cells, undergoes early epithelial rearrangements, including transient stratification of an initially monolayered epithelium, followed by microlumen formation and later resolution into branches. Within the epithelium, a multipotent progenitor cell (MPC) population is specified, giving rise to three important lineages: acinar, ductal and endocrine. Pdx1 is a transcription factor required for pancreas development and lineage specification; however, few Pdx1 targets that regulate pancreatogenesis have been identified. We find that pancreatic defects in Pdx1−/− embryos initiate at the time when the progenitor pool is specified and the epithelium should resolve into branches. Pdx1−/− microlumen diameters expand aberrantly, resulting in failure of epithelial tubulogenesis and ductal plexus formation. Pdx1−/− epithelial cell proliferation is decreased and the MPC pool is rapidly lost. We identify two conserved Pdx1 binding sites in the epithelial cadherin (E-cad, Cdh1) promoter, and show that Pdx1 directly binds and activates E-cad transcription. In addition, Pdx1 is required in vivo for maintenance of E-cad expression, actomyosin complex activity and cell shape. These findings demonstrate a novel link between regulators of epithelial architecture, specification of pancreatic cell fate and organogenesis. Summary: Mouse Pdx1 depletion in the pancreas leads to aberrant cell shape, impaired ductal tubulogenesis and loss of the epithelial multipotent progenitor cell population.
Systematic review of prostate cancer risk and association with consumption of fish and fish‐oils: analysis of 495,321 participants

Fish‐oils have a potential role in inflammation, carcinogenesis inhibition and favourable cancer outcomes. There has been increasing interest in the relationship of diet with cancer incidence and mortality, especially for eicosapantaenoic acid (EPA) and docosahexaenoic acid (DHA). This systematic‐analysis of the literature aims to review evidence for the roles of dietary‐fish and fish‐oil intake in prostate‐cancer (PC) risk, aggressiveness and mortality.
Did electricity drive Spain’s “most progressive decade”?

Following the growth accounting approach introduced by Oliner & Sichel (2000, 2002) to evaluate the impact of information and communications technologies on the U.S. economy in the 1990s, this paper analyses the impact of electricity on Spanish economic growth in the period 1958-1970. Spain was a follower country that exhibited the benefits of electricity nearly half a century after it had its biggest impact in the U.S. The results confirm that electricity played a significant role in Spain via the three channels identified in the literature for quantifying the contribution of a general purpose technology (GPT): capital deepening, the total factor productivity effect and the spillover effect. The overall impact is greater than that estimated for other follower countries in the 1920s. The main boost to growth came from improvements in productivity in developments in electric plants electricity and the production of electrical capital goods, not from electricity use. We also find a weaker positive effect of spillovers in electricity-using industries. The laggard effect of electrification in Spain, in spite of its early start, confirms that a GPT needs time to establish new institutional arrangements and complementary investments in order to display positive linkages deriving from the new technology.