Miracle-dz/UniFAR-FTUs huggingface dataset:
The reliability of MinimaxX accelerometers for measuring physical activity in Australian football.

PURPOSE To assess the reliability of triaxial accelerometers as a measure of physical activity in team sports. METHODS Eight accelerometers (MinimaxX 2.0, Catapult, Australia) were attached to a hydraulic universal testing machine (Instron 8501) and oscillated over two protocols (0.5 g and 3.0 g) to assess within- and between device reliability. A static assessment was also conducted. Secondly, 10 players were instrumented with two accelerometers during Australian football matches. The vector magnitude was calculated, expressed as Player load and assessed for reliability using typical error (TE) ± 90% confidence intervals (CI), and expressed as a coefficient of variation (CV%). The smallest worthwhile difference (SWD) in Player load was calculated to determine if the device was capable of detecting differences in physical activity. RESULTS Laboratory: Within- (Dynamic: CV 0.91 to 1.05%; Static: CV 1.01%) and between-device (Dynamic: CV 1.02 to 1.04%; Static: CV 1.10%) reliability was acceptable across each test. Field: The between-device reliability of accelerometers during Australian football matches was also acceptable (CV 1.9%). The SWD was 5.88%. CONCLUSIONS The reliability of the MinimaxX accelerometer is acceptable both within and between devices under controlled laboratory conditions, and between devices during field testing. MinimaxX accelerometers can be confidently utilized as a reliable tool to measure physical activity in team sports across multiple players and repeated bouts of activity. The noise (CV%) of Player load was lower than the signal (SWD), suggesting that accelerometers can detect changes or differences in physical activity during Australian football.
MinC Protein Shortens FtsZ Protofilaments by Preferentially Interacting with GDP-bound Subunits*

Background: The MinC protein of the site selection Min system targets FtsZ to prevent polar division ring formation. Results: At similar MinC concentrations, MinC protein shortens FtsZ protofilaments and interacts preferentially with GDP-bound FtsZ. Conclusion: MinC disrupts FtsZ protofilaments by specifically targeting FtsZ-GDP subunits. Significance: Biophysical analysis reveals how MinC disrupts FtsZ filaments in solution. The interaction of MinC with FtsZ and its effects on FtsZ polymerization were studied under close to physiological conditions by a combination of biophysical methods. The Min system is a widely conserved mechanism in bacteria that ensures the correct placement of the division machinery at midcell. MinC is the component of this system that effectively interacts with FtsZ and inhibits the formation of the Z-ring. Here we report that MinC produces a concentration-dependent reduction in the size of GTP-induced FtsZ protofilaments (FtsZ-GTP) as demonstrated by analytical ultracentrifugation, dynamic light scattering, fluorescence correlation spectroscopy, and electron microscopy. Our experiments show that, despite being shorter, FtsZ protofilaments maintain their narrow distribution in size in the presence of MinC. The protein had the same effect regardless of its addition prior to or after FtsZ polymerization. Fluorescence anisotropy measurements indicated that MinC bound to FtsZ-GDP with a moderate affinity (apparent KD ∼10 μm at 100 mm KCl and pH 7.5) very close to the MinC concentration corresponding to the midpoint of the inhibition of FtsZ assembly. Only marginal binding of MinC to FtsZ-GTP protofilaments was observed by analytical ultracentrifugation and fluorescence correlation spectroscopy. Remarkably, MinC effects on FtsZ-GTP protofilaments and binding affinity to FtsZ-GDP were strongly dependent on ionic strength, being severely reduced at 500 mm KCl compared with 100 mm KCl. Our results support a mechanism in which MinC interacts with FtsZ-GDP, resulting in smaller protofilaments of defined size and having the same effect on both preassembled and growing FtsZ protofilaments.
Screening Yield of HIV Antigen/Antibody Combination and Pooled HIV RNA Testing for Acute HIV Infection in a High-Prevalence Population.

IMPORTANCE Although acute HIV infection contributes disproportionately to onward HIV transmission, HIV testing has not routinely included screening for acute HIV infection. OBJECTIVE To evaluate the performance of an HIV antigen/antibody (Ag/Ab) combination assay to detect acute HIV infection compared with pooled HIV RNA testing. DESIGN, SETTING, AND PARTICIPANTS Multisite, prospective, within-individual comparison study conducted between September 2011 and October 2013 in 7 sexually transmitted infection clinics and 5 community-based programs in New York, California, and North Carolina. Participants were 12 years or older and seeking HIV testing, without known HIV infection. EXPOSURES All participants with a negative rapid HIV test result were screened for acute HIV infection with an HIV Ag/Ab combination assay (index test) and pooled human immunodeficiency virus 1 (HIV-1) RNA testing. HIV RNA testing was the reference standard, with positive reference standard result defined as detectable HIV-1 RNA on an individual RNA test. MAIN OUTCOMES AND MEASURES Number and proportion with acute HIV infections detected. RESULTS Among 86,836 participants with complete test results (median age, 29 years; 75.0% men; 51.8% men who have sex with men), established HIV infection was diagnosed in 1158 participants (1.33%) and acute HIV infection was diagnosed in 168 participants (0.19%). Acute HIV infection was detected in 134 participants with HIV Ag/Ab combination testing (0.15% [95% CI, 0.13%-0.18%]; sensitivity, 79.8% [95% CI, 72.9%-85.6%]; specificity, 99.9% [95% CI, 99.9%-99.9%]; positive predictive value, 59.0% [95% CI, 52.3%-65.5%]) and in 164 participants with pooled HIV RNA testing (0.19% [95% CI, 0.16%-0.22%]; sensitivity, 97.6% [95% CI, 94.0%-99.4%]; specificity, 100% [95% CI, 100%-100%]; positive predictive value, 96.5% [95% CI, 92.5%-98.7%]; sensitivity comparison, P < .001). Overall HIV Ag/Ab combination testing detected 82% of acute HIV infections detectable by pooled HIV RNA testing. Compared with rapid HIV testing alone, HIV Ag/Ab combination testing increased the relative HIV diagnostic yield (both established and acute HIV infections) by 10.4% (95% CI, 8.8%-12.2%) and pooled HIV RNA testing increased the relative HIV diagnostic yield by 12.4% (95% CI, 10.7%-14.3%). CONCLUSIONS AND RELEVANCE In a high-prevalence population, HIV screening using an HIV Ag/Ab combination assay following a negative rapid test detected 82% of acute HIV infections detectable by pooled HIV RNA testing, with a positive predictive value of 59%. Further research is needed to evaluate this strategy in lower-prevalence populations and in persons using preexposure prophylaxis for HIV prevention.
Molecular Analysis of the Subgingival Microbiota in Health and Disease

ABSTRACT This investigation provides molecular analyses of the periodontal microbiota in health and disease. Subgingival samples from 47 volunteers with healthy gingivae or clinically diagnosed chronic periodontitis were characterized by PCR-denaturing gradient gel electrophoresis (DGGE) with primers specific for the V2-V3 region of the eubacterial 16S rRNA gene. A hierarchical dendrogram was constructed from band patterns. All unique PCR amplicons (DGGE bands) were sequenced for identity. Samples were also analyzed for the presence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis by multiplex PCR. Associations of patient age, gender, and smoking status together with the presence of each unique band and putative periodontal pathogens with disease were assessed by logistic regression. Periodontal pockets were colonized by complex eubacterial communities (10 to 40 distinct DGGE bands) with substantial individual variation in the community profile. Species diversity in health and disease was determined by the Shannon-Weaver index of diversity and compared by the Mann-Whitney U test. Sequence analyses of DGGE amplicons indicated the occurrence of many nontypical oral species and eubacteria previously associated with this environment. With the exception of T. forsythensis, the putative pathogens were not detected by DGGE. Multiplex PCR, however, detected T. forsythensis, A. actinomycetemcomitans, and P. gingivalis in 9% 16%, and 29% of the patients with disease, respectively. The presence of A. actinomycetemcomitans was significantly associated with disease (P < 0.01). Statistical analyses indicated that the presence of Treponema socranskii and Pseudomonas sp. was a significant predictor of disease (P < 0.05) and that there was no significant difference (P > 0.05) in terms of eubacterial species diversity between health and disease.
New L‐Dopa Codrugs as Potential Antiparkinson Agents

This paper reports the synthesis and preliminary evaluation of new L‐dopa (LD) conjugates (1 and 2) obtained by joining LD with two different natural antioxidants, caffeic acid and carnosine, respectively. The antioxidant efficacy of compounds 1 and 2 was assessed by evaluating plasmatic activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the rat. Rat striatal concentration of LD and dopamine (DA), and central nervous effects were evaluated after oral administration of the codrugs 1 and 2. The results suggest that, though our codrugs are devoid of significant antioxidant activity, they are able to induce sustained delivery of DA in rat striatum and can improve LD and DA release in the brain.
The Role of Wheat Flour Constituents, Sugar, and Fat in Low Moisture Cereal Based Products: A Review on Sugar-Snap Cookies

Much research has been done to understand the contribution of different flour constituents to the cookie quality. Most authors agree on the role of starch in cookies, which, although it is the main flour constituent, has a relatively small influence on cookie quality. Flour proteins, which are quantitatively less important than starch, seem to have a more pronounced role in cookie baking. However, in literature, there is no consensus about their role and influence on the product quality. As for starch, there is much more agreement about the role of non-starch polysaccharides and flour lipids. Not only flour, but also other ingredients of the cookie (dough) formula, such as shortening (fat), sugar, and water are important for the quality of the end product. We here provide the different points of view in this area and speculate on the functionality and quality determining properties of flour constituents, sugar, fat, and water and their role and influence during the different stages of cookie baking and on the end quality of sugar-snap cookies.
The dilemma of the metabolic syndrome in children and adolescents: disease or distraction?

Background: The alarming increase of obesity in children occurring globally is raising concern about the implications for development, at earlier ages, of atherosclerosis and cardiovascular disease and is driving efforts to evaluate and predict risk in the young. A major component of this undertaking has been directed to adapting for children the criteria described in adults as the metabolic syndrome (MS).
Economic Assessment of Network-Constrained Transactive Energy for Managing Flexible Demand in Distribution Systems

The increasing number of distributed energy resources such as electric vehicles and heat pumps connected to power systems raises operational challenges to the network operator, for example, introducing grid congestion and voltage deviations in the distribution network level if their operations are not properly coordinated. Coordination and control of a large number of distributed energy resources requires innovative approaches. In this paper, we follow up on a recently proposed network-constrained transactive energy (NCTE) method for scheduling of electric vehicles and heat pumps within a retailer’s aggregation at distribution system level. We extend this method with: (1) a new modeling technique that allows the resulting congestion price to be directly interpreted as a locational marginal pricing in the system; (2) an explicit analysis of the benefits and costs of different actors when using the NCTE method in the system, given the high penetration of distributed energy resources. This paper firstly describes the NCTE-based distribution system that introduces a new interacting scheme for actors at the distribution system level. Then, technical modeling and economic interpretation of the NCTE-based distribution system are described. Finally, we show the benefits and costs of different actors within the NCTE-based distribution system.
Choice and Matching

We study path-independent choice rules applied to a matching context. We use a classic representation of these choice rules to introduce a powerful technique for matching theory. Using this technique, we provide a deferred acceptance algorithm for many-to-many matching markets with contracts and study its properties. Next, we obtain a compelling comparative static result: if one agent's choice expands, the remaining agents on her side of the market are made worse off, while agents on the other side of the market are made better off. Finally, we establish several results related to path-independent choice rules.
Aspects of Canadian and US Business Cycles

The Canadian and US economies, by many measures and along many dimensions, are highly integrated and, possibly as a result of this integration, have highly correlated business cycles. This paper examines various aspects of the co-movement between the two economies. One objective is to document the relationship between the two economies at an aggregate level and to examine how the relationship might have changed over time. A further objective is to explore the relationship at a more disaggregate level by examining the interaction of the two economies on a sectoral basis.
Functional Characterization of Three Aquaglyceroporins from Trypanosoma brucei in Osmoregulation and Glycerol Transport

Previous studies using bloodstream form Trypanosoma brucei have shown that glycerol transport in this parasite occurs via specific membrane proteins, namely a glycerol transporter and glycerol channels [1]. Later, we cloned, expressed and characterized the transport properties of all three aquaglyceroporins (AQP1-3) [2], which were found permeable for water, glycerol and other small uncharged solutes like dihydroxyacetone [3]. Here, we report on the cellular localization of TbAQP1 and TbAQP3 in bloodstream form trypanosomes. Indirect immunofluorescence analysis showed that TbAQP1 is exclusively localized in the flagellar membrane, whereas TbAQP3 was found in the plasma membrane.In addition, we analyzed the functions of all 3 AQPs, using an inducible inheritable double-stranded RNA interference methodology. All AQP knockdown cell lines were still able to survive hypo-osmotic stress conditions, except AQP2 knockdown parasites. Depleted TbAQP2 negatively impacted cell growth and the regulatory volume recovery, whereas AQP1 und 3 knockdown trypanosomes displayed phenotypes consistent with their localization in external membranes. A simultaneous knockdown of all 3 AQPs showed that the cells were able to substitute the missing glycerol uptake capability through a putative glycerol transporter.
Design and Implementation of Digital TV Integrated Business Support System

Integrated Business Support System of digital TV, also called Subscriber Management System(SMS), includes subscriber management, program purchase, balancing charges, engineering construction management. It also transmits commands of assignment or cancellation of authorization to CA system by interfacing with CA, which then passes these commands to scrambler, and control of acceptance of Digital TV program is accomplished. Unique CA is required over a whole province, program streams, SI, ECM, EMM are transmitted through SDH network. This paper discusses its operation mode, software architecture and network services of SMS.
Discrimination at the Intersection of Age, Race, and Gender: Evidence from a Lab-in-The-Field Experiment

We use a laboratory experiment with randomized resumes and eyetracking to explore the effects of race on employment discrimination over the lifecycle. We show race discrimination against prime-age black job applicants that diminishes into middle age before re-emerging for older applicants. Screeners mechanically process black and white resumes similarly, but spend less time on younger black resumes, suggesting they use negative heuristics or taste-based discrimination. Screeners demonstrate levels-based statistical discrimination, believing that younger black applicants have worse computer skills and more gaps in their job histories. We find no evidence that screeners believe black applicants have worse previous experience. Screeners demonstrate variance-based statistical discrimination against black applicants of all ages, suggesting that screeners perceive the stronger history signals for white applicants, with this type of discrimination disproportionately affecting older applicants. We find suggestive evidence that the signal sent by high school attended is weaker for younger black applicants compared to younger white applicants, and we find no evidence that the signal strength of the applicant’s address varies by race. Evidence from the CPS and an additional study supports the external validity of our experiment, particularly for female job applicants. Results are robust to different controls and specification choices.
Proof-of-Possession Key Semantics for JSON Web Tokens (JWTs)

This specification describes how to declare in a JSON Web Token (JWT) that the presenter of the JWT possesses a particular proof-of- possession key and how the recipient can cryptographically confirm proof of possession of the key by the presenter. Being able to prove possession of a key is also sometimes described as the presenter being a holder-of-key.
Collaborative Post-IDS Alert Analysis Based on Network Management Techniques

These days, IDS has been widely deployed and is beginning to gain acceptance as a worthwhile investment. However, at least three disadvantages, which are very high alert rate, fairly high false positives and quite limited ability for detecting distributed and collaborative attacks, hinder its further development. In order to overcome these weaknesses and resolve the conflict with current network security requirements as well, researches on post-IDS alert analysis become a focus, while more and more researchers and engineers advocate two techniques named collaboration and correlation. The aim of this paper is then to propose application of XML-based integrated network management techniques to support collaboration for the purpose of post-IDS alert analysis, and discuss corresponding issues about unified representation of information and knowledge. Validation results show that, proposed approach is quite effective in reducing the rate of false positives.
Invariant HPD credible sets and MAP estimators

MAP estimators and HPD credible sets are often criticized in the literature because of paradoxical behaviour due to a lack of equivariance under reparametrization. In this paper, we propose a new version of MAP estimators and HPD credible sets that avoid this undesirable feature. Moreover, in the special case of non-informative prior, the new MAP estimators coincide with the equivariant frequentist ML estimators. We also propose several adaptations in the case of nuisance parameters.
"Reactive filtration": use of functionalized porous polymer monoliths as scavengers in solution-phase synthesis.

[reaction: see text] Solid functionalized porous monolithic disks with reactive polymer chains grafted to their inner pore surface have been developed for scavenging excess reagents from reaction mixtures. A poly(chloromethylstyrene-co-divinylbenzene) monolith was cut into disks and activated by graft polymerizing 4-vinyl-2,2-dimethylazlactone to its pore surface. In contrast to the direct copolymerization of reactive monomers, grafting increases the accessibility of the reactive groups. Application of the reactive disks is demonstrated in the scavenging of excess amines from reaction mixtures in different solvents.
Scopolamine Impairs Acquisition and Facilitates Consolidation of Fear Conditioning: Differential Effects for Tone vs Context Conditioning

Cholinergic antagonism impacts selected learning tasks. To understand where scopolamine exerts its action, learning tasks differentially sensitive to hippocampus and amygdala lesions were used. Hippocampal lesions prevent context fear conditioning without effect on tone conditioning. These lesions also produce a time-dependent retrograde deficit in context conditioning. The amygdala is necessary for both tone and context conditioning. To examine the possibility that cholinergic antagonism mimics hippocampal damage or amygdala damage, rats were given scopolamine (1 mg/kg) either before or after fear conditioning. In the fear conditioning procedure, rats received tone-footshock or context-footshock pairings. Evidence of conditioning to the tone and the context was provided by observation of freezing. When given prior to training, scopolamine blocked fear conditioning to the tone in a dose-dependent fashion but had no effect on context conditioning. The impairment of tone conditioning did not occur with methylscopolamine, indicating the central action of the drug. Rats given scopolamine immediately following fear conditioning, tested later in a drug-free state, froze more to the tone than rats given a control injection. The effect of scopolamine on freezing to the context was not reliable. The present results suggest that scopolamine's impact on fear conditioning is mediated by some mechanism other than impaired hippocampal or amygdala functioning.
The Tetraspanin CD63 Is Required for Efficient IgE-Mediated Mast Cell Degranulation and Anaphylaxis

Mast cell (MC) activation through the high-affinity IgE receptor FcεRI leads to the release of mediators involved in immediate-type allergic reactions. Although Abs against the tetraspanins CD63 and CD81 inhibit FcεRI-induced MC degranulation, the intrinsic role of these molecules in FcεRI-induced MC activation is unknown. In MCs, CD63 is expressed at the cell surface and in lysosomes (particularly secretory lysosomes that contain allergic mediators). In this study, we investigated the role of CD63 in MC using a CD63 knockout mouse model. CD63-deficiency did not affect in vivo MC numbers and tissue distribution. Bone marrow–derived MC developed normally in the absence of CD63 protein. However, CD63-deficient bone marrow–derived MC showed a significant decrease in FcεRI-mediated degranulation, but not PMA/ionomycin-induced degranulation, as shown by β-hexosaminidase release assays. The secretion of TNF-α, which is both released from granules and synthesized de novo upon MC activation, was also decreased. IL-6 secretion and production of the lipid mediator leukotriene C4 were unaffected. There were no ultrastructural differences in granule content and morphology, late endosomal/lysosomal marker expression, FcεRI-induced global tyrosine phosphorylation, and Akt phosphorylation. Finally, local reconstitution in genetically MC-deficient Kitw/w-v mice was unaffected by the absence of CD63. However, the sites reconstituted with CD63-deficient MC developed significantly attenuated cutaneous anaphylactic reactions. These findings demonstrate that the absence of CD63 results in a significant decrease of MC degranulation, which translates into a reduction of acute allergic reactions in vivo, thus identifying CD63 as an important component of allergic inflammation.