Miracle-dz/UniFAR-FTUs huggingface dataset:
PET∕CT imaging evidence of FUS-mediated (18)F-FDG uptake changes in rat brain.

PURPOSE Transcranial focused ultrasound (FUS) delivers highly focused acoustic energy to a small region of the brain in a noninvasive manner. Recent studies have revealed that FUS, which is administered either in pulsed or continuous waves, can elicit or suppress neural tissue excitability. This neuromodulatory property of FUS has been demonstrated via direct motion detection, electrophysiological recordings, functional magnetic resonance imaging (fMRI), confocal imaging, and microdialysis sampling of neurotransmitters. This study presents new evidence of local increase in glucose metabolism induced by FUS to the rat brain using FDG (18-fludeoxyglucose) positron emission tomography (PET). METHODS Sprague-Dawley rats underwent sonication to a unilateral hemispheric area of the brain prior to PET scan. The pulsed sonication (350 kHz, tone burst duration of 0.5 ms, pulse repetition frequency of 1 kHz, and duration of 300 ms) was applied in 2 s intervals for 40 min immediately after the FDG injection via tail vein. Subsequently, the PET was acquired in dynamic list-mode to image FDG activity for an hour, and reconstructed into a single volume representing standardized uptake value (SUV). The raw SUV as well as its asymmetry index (AI) were measured from five different volume-of-interests (VOIs) of the brain for both hemispheres, and compared between sonicated and unsonicated groups. RESULTS Statistically significant hemispheric changes in SUV were observed only at the center of sonication focus within the FUS group [paired t-test; t(7) = 3.57, p < 0.05]. There were no significant hemispheric differences in SUV within the control group in any of the VOIs. A statistically significant elevation in AI (t-test; t(7) = 3.40, p < 0.05) was observed at the center of sonication focus (7.9 ± 2.5%, the deviations are in standard error) among the FUS group when compared to the control group (-0.8 ± 1.2%). CONCLUSIONS Spatially distinct increases in the glucose metabolic activity in the rat brain is present only at the center of sonication focus, suggesting localized functional neuromodulation mediated by the sonication.
Lysines Close to the Rous Sarcoma Virus Late Domain Critical for Budding

ABSTRACT The release of retroviruses from the plasma membrane requires host factors that are believed to be recruited to the site of budding by the late (L) domain of the virus-encoded Gag protein. The L domain of Rous sarcoma virus (RSV) has been shown to interact with a ubiquitin (Ub) ligase, and budding of this virus is dependent on Ub. RSV is similar to other retroviruses in that it contains ∼100 molecules of Ub, but it is unique in that none of these molecules has been found to be conjugated to Gag. If transient ubiquitination of RSV Gag is required for budding, then replacement of the target lysine(s) with arginine should prevent the addition of Ub and reduce budding. Based on known sites of ubiquitination in other viruses, the important lysines would likely reside near the L domain. In RSV, there are five lysines located just upstream of the L domain in a region of the matrix (MA) protein that is dispensable for membrane binding, and replacement of these with arginine (mutant 1-5KR) reduced budding 80 to 90%. The block to budding was found to be on the plasma membrane; however, the few virions that were released had normal size, morphology, and infectivity. Budding was restored when any one of the residues was changed back to lysine or when lysines were inserted in novel positions, either within this region of MA or within the downstream p10 sequence. Moreover, the 1-5KR mutant could be rescued into particles by coexpression of budding-competent Gag molecules. These data argue that the phenotype of mutant 1-5KR is not due to a conformational defect. Consistent with the idea that efficient budding requires a specific role for lysines, human T-cell leukemia virus type 1, which does not bud well compared to RSV and lacks lysines close to its L domain, was found to be released at a higher level upon introduction of lysines near its L domain. This report strongly supports the hypothesis that ubiquitination of the RSV Gag protein (and perhaps those of other retroviruses) is needed for efficient budding.
Less is more: Increasing the scope of hardware debugging with compression

In this work we consider the slow and tedious phase of hardware debugging in FPGAs. The process of hardware debugging is normally done via Internal Logic Analyzer (ILA) circuits, which add user observability in internal FPGA signals. The user first defines the target for debugging signal and a triggering condition. Then the ILA stores traces of it in trace buffers, these traces are finally transferred to a host PC for the user to observe. The user also has to consider the limited FPGA memory resources, which result in small-sized trace buffers, an important restriction of hardware debugging. In this paper, we attempt to increase the scope of hardware tracing, i.e. the number of samples written on the trace buffers, with the use of compression. We use the LZW algorithm and find that we can increase the debugging signals number of recorded samples by 90%, i.e. have double the amount of useful data with the same memory usage. On the memory plane we conclude that, for the same number of recorded samples, our architecture uses less than half the resources compared to the standard Xilinx ILA block for signal tracing.
Object-oriented Application Object-oriented Application Frameworks

promising technology for reifying proven software designs and implementations in order to reduce the cost and improve the quality of software. A framework is a reusable, “semi-complete’’ application that can be specialized to produce custom applications [7, 10]. In contrast to earlier OO reuse techniques based on class libraries, frameworks are targeted for particular business units (such as data processing or cellular communications) and application domains (such as user interfaces or real-time avionics) [11]. Frameworks like MacApp, ET++, Interviews, ACE, Microsoft’s MFC and DCOM, JavaSoft’s RMI, and implementations of OMG’s CORBA play an increasingly important role in contemporary software development. The primary benefits of OO application frameworks stem from the modularity, reusability, extensibility, and inversion of control they provide to developers. Frameworks enhance modularity by encapsulating volatile implementation details behind stable interfaces. Framework modularity helps improve software quality by localizing the impact of design and implementation changes, which reduces the effort required Mohamed E. Fayad and Douglas C. Schmidt, Guest Editors
The GPR54 gene as a regulator of puberty.

BACKGROUND Puberty, a complex biologic process involving sexual development, accelerated linear growth, and adrenal maturation, is initiated when gonadotropin-releasing hormone begins to be secreted by the hypothalamus. We conducted studies in humans and mice to identify the genetic factors that determine the onset of puberty. METHODS We used complementary genetic approaches in humans and in mice. A consanguineous family with members who lacked pubertal development (idiopathic hypogonadotropic hypogonadism) was examined for mutations in a candidate gene, GPR54, which encodes a G protein-coupled receptor. Functional differences between wild-type and mutant GPR54 were examined in vitro. In parallel, a Gpr54-deficient mouse model was created and phenotyped. Responsiveness to exogenous gonadotropin-releasing hormone was assessed in both the humans and the mice. RESULTS Affected patients in the index pedigree were homozygous for an L148S mutation in GPR54, and an unrelated proband with idiopathic hypogonadotropic hypogonadism was determined to have two separate mutations, R331X and X399R. The in vitro transfection of COS-7 cells with mutant constructs demonstrated a significantly decreased accumulation of inositol phosphate. The patient carrying the compound heterozygous mutations (R331X and X399R) had attenuated secretion of endogenous gonadotropin-releasing hormone and a left-shifted dose-response curve for gonadotropin-releasing hormone as compared with six patients who had idiopathic hypogonadotropic hypogonadism without GPR54 mutations. The Gpr54-deficient mice had isolated hypogonadotropic hypogonadism (small testes in male mice and a delay in vaginal opening and an absence of follicular maturation in female mice), but they showed responsiveness to both exogenous gonadotropins and gonadotropin-releasing hormone and had normal levels of gonadotropin-releasing hormone in the hypothalamus. CONCLUSIONS Mutations in GPR54, a G protein-coupled receptor gene, cause autosomal recessive idiopathic hypogonadotropic hypogonadism in humans and mice, suggesting that this receptor is essential for normal gonadotropin-releasing hormone physiology and for puberty.
Long‐term effects of compost amendment of soil on functional and structural diversity and microbial activity

We studied the effects of applying different composts (urban organic waste, green waste, manure and sewage sludge), mineral fertilizer and compost plus mineral fertilizer on chemical, biological and soil microbiological parameters over a 12‐year period. The organic C and total N levels in soils were increased by all compost and compost + N treatments. Microbial biomass C was significantly (P ≤ 0.05) increased for some compost treatments. In addition, basal respiration and the metabolic quotient (qCO2) were significantly higher in all soils that had received sewage sludge compost. The Shannon diversity index (H), based on community level physiological profiling, showed a higher consumption of carbon sources in soils treated with compost and compost + N compared with the control. The utilization of different guilds of carbon sources varied amongst the treatments (compost, compost + N or mineral fertilizer). Cluster analysis of polymerase chain reaction‐denaturing gradient gel electrophoresis patterns showed two major clusters, the first containing the mineral fertilization and compost treatments, and the second, the composts + N treatments. No differences in bacterial community structure could be determined between the different types of compost. However, the results suggest that long‐term compost treatments do have effects on the soil biota. The results indicate that the effects on the qCO2 may be due to shifts in community composition. In this study, it was not possible to distinguish with certainty between the effects of different composts except for compost derived from sewage sludge.
An Update on the Important Findings from the EUROSTAR EVAR Registry

This article summarizes the important findings to emerge from the European Collaborators on Stent-Graft Techniques for Abdominal Aortic Aneurysm Repair (EUROSTAR) Registry since its inception in 1996. The Registry is an open observational study of the outcomes from endovascular aneurysm repair (EVAR). Data have been contributed, on a voluntary basis, by 135 vascular centers from 18 European countries. Patients are registered prospectively, and data analysis is on an intention-to-treat basis. Results from the first generations of endograft were characterized by excellent early results but poor durability of abdominal aortic aneurysm repair. EUROSTAR data have played a vital role in informing the technical evolution of subsequent generations of endograft and their clinical application. The latest data from EUROSTAR show significant improvements in all outcome measures, most importantly, those relating to durability. EUROSTAR has tracked and informed the evolution of EVAR. Improving results confirm that substantial progress has been made since 1996. EUROSTAR continues to contribute to this ongoing process.
Language in the military workplace—between hierarchy and politeness

Abstract Politeness is a pervasive aspect of communication and plays an important part in all our interactions (see, e.g., Brown and Levinson, Politeness: Some universals in language usage, Cambridge University Press, 1987). Communication in the military is no exception. The differences which exist are examined in this article by observing interactions in a US army battalion. The main focus is the language used in office situations based on a questionnaire filled out by members of the battalion, interviews, and several days of field study. The interpretation and analysis of these data detail how military personnel is supposed to and does deal with both superiors and subordinates in a variety of speech act situations, such as how directives and complaints are formulated, how criticism is voiced, and how advice is given to superiors, peers, and subordinates. The results show that respectful behavior, on the one hand, is prescribed by regulations and institutions and, on the other hand, it is shaped by the individual's creativity and an informal and familiar style. Thus, the military in non-combat office situations has its peculiarities in language use in such areas as rank, but also makes use of typical features of workplace talk such as the mitigation of directives.
A pairwise key pre-distribution scheme for wireless sensor networks

To achieve security in wireless sensor networks, it is important to be able to encrypt and authenticate messages sent among sensor nodes. Keys for encryption and authentication purposes must be agreed upon by communicating nodes. Due to resource constraints, achieving such key agreement in wireless sensor networks is non-trivial. Many key agreement schemes used in general networks, such as Diffie-Hellman and public-key based schemes, are not suitable for wireless sensor networks. Pre-distribution of secret keys for all pairs of nodes is not viable due to the large amount of memory used when the network size is large. To solve the key pre-distribution problem, two elegant key pre-distribution approaches have been proposed recently [11, 7]. In this paper, we propose a new key pre-distribution scheme, which substantially improves the resilience of the network compared to the existing schemes. Our scheme exhibits a nice threshold property: when the number of compromised nodes is less than the threshold, the probability that any nodes other than these compromised nodes is affected is close to zero. This desirable property lowers the initial payoff of smaller scale network breaches to an adversary, and makes it necessary for the adversary to attack a significant proportion of the network. We also present an in depth analysis of our scheme in terms of network resilience and associated overhead.
Student Evaluation of Teaching: Methodological Critique

The purpose of the present work is twofold. The first is to outline two arguments that challenge those who would advocate a continuation of the exclusive use of raw SET data in the determination of "teaching effectiveness" in the "summative" function. The second purpose is to answer this question: "In the face of such challenges, why do university administrators continue to use these data exclusively in the determination of 'teaching effectiveness'? "
Burkholderia pseudomallei Type III Secretion System Cluster 3 ATPase BsaS, a Chemotherapeutic Target for Small-Molecule ATPase Inhibitors

ABSTRACT Melioidosis is an infectious disease of high mortality for humans and other animal species; it is prevalent in tropical regions worldwide. The pathogenesis of melioidosis depends on the ability of its causative agent, the Gram-negative bacterium Burkholderia pseudomallei, to enter and survive in host cells. B. pseudomallei can escape from the phagosome into the cytosol of phagocytic cells where it replicates and acquires actin-mediated motility, avoiding killing by the autophagy-dependent process, LC3 (microtubule-associated protein light chain 3)-associated phagocytosis (LAP). The type III secretion system cluster 3 (TTSS3) facilitates bacterial escape from phagosomes, although the mechanism has not been fully elucidated. Given the recent identification of small-molecule inhibitors of the TTSS ATPase, we sought to determine the potential of the predicted TTSS3 ATPase, encoded by bsaS, as a target for chemotherapeutic treatment of infection. A B. pseudomallei bsaS deletion mutant was generated and used as a control against which to assess the effect of inhibitor treatment. Infection of RAW 264.7 cells with wild-type bacteria and subsequent treatment with the ATPase inhibitor compound 939 resulted in reduced intracellular bacterial survival, reduced escape from phagosomes, and increased colocalization with both LC3 and the lysosomal marker LAMP1 (lysosome-associated membrane protein 1). These changes were similar to those observed for infection of RAW 264.7 cells with the bsaS deletion mutant. We propose that treatment with the ATPase inhibitor compound 939 decreased intracellular bacterial survival through a reduced ability of bacteria to escape from phagosomes and increased killing via LAP. Therefore, small-molecule inhibitors of the TTSS3 ATPase have potential as therapeutic treatments against melioidosis.
Exploring Teachers' Perception of the Efficacy of ELT in Iranian Public Schools and Private Language Institutes.

This paper intended to compare teaching English in private language institutes and public schools in Iranian Education system from the viewpoint of the English teachers who practice in both systems. It also aimed at examining teachers’ preference to teach-in either place. 15 English teachers in Ilam city participated in the study and data were collected through a researcher-mad e questionnaire, including 60 Items through which respondents were asked to express their dis/agreement on 5 subscales. The results showed that better management, better fulfillment of the educational needs, and better teaching environment were among the reasons why the teachers preferred to teach in private language institutes. The study then, provides suggestions for improvement of ELT in the public system.
Self-Reported Prevalence of Chronic Diseases and Their Relation to Selected Sociodemographic Variables: A Study in INDEPTH Asian Sites, 2005

Introduction Lack of reliable population-based data, especially morbidity data, is a barrier to preventing and controlling chronic diseases in developing countries. We report the self-reported prevalences of major chronic diseases in Southeast Asia and examine their relation to selected sociodemographic variables in adults. Methods Data are from a 2005 cross-site study of 8 sites in 5 Asian countries that surveyed 18,484 people aged 25–64 years. Respondents were asked whether they had been told by a health care worker that they had any of 7 chronic health conditions: joint problems, stroke, heart disease, diabetes, pulmonary disease, hypertension, or cancer. Information about participants' sex, age, and educational level was also obtained. Results We found that 22.7% of men and 31.6% of women reported having at least 1 of the chronic health conditions of interest, and 5.1% of men and 9.2% of women reported having 2 or more chronic conditions. Multivariate regression analyses showed that women had more chronic conditions than men, the prevalence of chronic conditions increased with age, and people with the least education were more likely to have chronic conditions. Conclusion Chronic conditions are commonly reported among adults in Asian countries. Disparities in the prevalence of chronic conditions by sex and education are evident.
The Black-White achievement gap: Do state policies matter?

A longstanding issue in American education is the gap in academic achievement between majority and minority students. The goal of this study is to accumulate and evaluate evidence on the relationship between state education policies and changes in the Black-White achievement gap, while addressing some of the methodological issues that have led to differences in interpretations of earlier findings. To that end, we consider the experiences of ten states that together enroll more than forty percent of the nation's Black students. We estimate the trajectories of Black student and White student achievement on the NAEP 8th grade mathematics assessment over the period 1992 to 2000, and examine the achievement gap at three levels of aggregation: the state as a whole, groups of schools (strata) within a state defined by the SES level of the student population, and within schools within a stratum within a state. From 1992 to 2000, at every level of aggregation, mean achievement rose for both Black students and White students. However, for most states the achievement gaps were large and changed very little at every level of aggregation. The gaps are pervasive, profound and persistent. There is substantial heterogeneity among states in the types of policies they pursued, as well as the coherence and consistency of those policies during the period 1988-1998. We find that states' overall policy rankings (based on our review of the data) correlate moderately with their record in improving Black student achievement but are somewhat less useful in predicting their record with respect to reducing the achievement gaps. States' rankings on commitment to teacher quality correlate almost as well as did the overall policy ranking. Thus, state reform efforts are a blunt tool, but a tool nonetheless. Our findings are consistent with the following recommendations: states' reform efforts should be built on broad-based support and buffered as much as possible from changes in budgets and politics; states should employ the full set of policy levers at their disposal; and policies should directly support local reform efforts with proven effectiveness in addressing the experiences of students of different races attending the same schools.
Quantitative tissue sodium concentration mapping of the growth of focal cerebral tumors with sodium magnetic resonance imaging

Tissue sodium concentration (TSC), as determined by in vivo 23Na magnetic resonance imaging (MRI) and the ex vivo classical 22Na radionuclide dilution assay (RDA), has been compared in a rat model of a focal glioma. The 23Na MRI method used a three‐dimensional, twisted projection acquisition scheme at short echo time to minimize signal losses from relaxation of transverse magnetization. Calibration standards within the field of view allowed quantification of the sodium signal in terms of a TSC after correction for B1 nonuniformity and tissue water concentration. The 23Na MRI method measured focally increased TSC values in tumors that were equivalent statistically to the destructive 22Na RDA method. The noninvasive 23Na MRI method provided a quantitative means with which to monitor focal brain tumor growth. Magn Reson Med 41:351–359, 1999. © 1999 Wiley‐Liss, Inc.
On the Cryptanalysis via Approximation of Cryptographic Primitives Relying on the Planted Clique Conjecture

While the reliable use of some NP-complete problem in tandem with the assumption that P is not equal to NP has eluded cryptographers due to lack of results showing average-case hardness, one alternative which has been explored is reliance on assumptions that solving certain NP-hard optimization problems within some degree of accuracy is computationally difficult in specific instance classes. In this work, we explore one such example of this effort which attempts to provide cryptographic primitives by relying on the planted clique conjecture. More specifically, we (1) present this construction in summary, (2) propose a simple cryptanalytic method using only approximation algorithms, and (3) consider the feasibility of such cryptanalysis in the context of existing approximation algorithms for the maximum clique problem. We ultimately find that recent advances in the area of combinatoric approximation algorithms fatally hinders the prospect of any serious application of existing candidate constructions based upon the planted clique conjecture.