Miracle-dz/UniFAR-FTUs huggingface dataset:
Radiosensitization of MDA-MB-231 breast tumor cells by adenovirus-mediated overexpression of a fragment of the XRCC4 protein

Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to inhibit the nonhomologous end joining repair pathway by interfering with the function of the XRCC4/ligase IV complex. A PCR-generated fragment of the XRCC4 gene, encompassing the homodimerization and ligase IV–binding domains, was inserted into a plasmid vector (pFLAG-CMV-2) expressing the FLAG peptide and the cassette encoding FLAG-tagged XRCC4 fragment was cloned into an adenoviral vector. Both the plasmid and the corresponding adenovirus elicited robust expression of a truncated XRCC4 protein designed to compete in a dominant-negative fashion with full-length XRCC4 for binding to ligase IV. Binding of the XRCC4 fragment to ligase IV in vivo was confirmed by immunoprecipitation. Clonogenic survival assays showed that the adenovirus expressing the truncated XRCC4 protein sensitizes MDA-MB-231 breast tumor cells to ionizing radiation, presumably through interference with the functional activity of ligase IV, leading to inhibition of the final ligation step in end joining. These studies support the potential clinical utility of combining radiation therapy with agents that inhibit DNA double-strand break repair.
Designs over the binary field from the complete monomial group

All known direct constructions of designs over finite fields arise by subgroups of the normalizer of a Singer cycle, by lifted special linear groups, or lifted general linear groups. Other types of non-trivial automorphism groups for which designs over finite fields do exist have not been mentioned so far. In this paper we construct the first non-trivial designs over finite fields admitting the lifted complete monomial group as group of automorphisms.
Involving High School Students in Computational Physics University Research: Theory Calculations of Toluene Adsorbed on Graphene

To increase public awareness of theoretical materials physics, a small group of high school students is invited to participate actively in a current research projects at Chalmers University of Technology. The Chalmers research group explores methods for filtrating hazardous and otherwise unwanted molecules from drinking water, for example by adsorption in active carbon filters. In this project, the students use graphene as an idealized model for active carbon, and estimate the energy of adsorption of the methylbenzene toluene on graphene with the help of the atomic-scale calculational method density functional theory. In this process the students develop an insight into applied quantum physics, a topic usually not taught at this educational level, and gain some experience with a couple of state-of-the-art calculational tools in materials research.
La gestion des connaissances

la realite de l'entreprise : la connaissance est un enjeu economique majeur de demain. Creer, capitaliser et partager son capital de connaissances est une preoccupation de toute organisation performante. Mais cerner ce qu'est vraiment la gestion des connaissances dans une organisation n'est pas une chose aisee, car elle en traverse presque toutes les composantes. Elle concerne la strategie, car il s'agit vraiment d'un nouveau type de management repondant a un nouvel environnement socio-economique et a une nouvelle vision de l'organisation. Elle concerne la structure de l'organisation, car le savoir se fait et se defait a travers des reseaux complexes, connectes a l'environnement, qui peuvent remettre en cause les structurations classiques. Elle concerne de nombreux processus deja mis en place dans les organisations (heureusement, de tout temps les etres humains ont gere leurs connaissances ! ), mais qu'il s'agit de revoir dans de nouvelles perspectives, d'optimiser ou de developper. Elle concerne le personnel de l'organisation, qui est au cœur de la problematique, tant il vrai que la connaissance ne se cree, ne se partage, n'evolue qu'a travers les personnes, qui doivent se mobiliser personnellement et collectivement pour cet objectif. Elle concerne les technologies de l'information et de la communication, qui sont des vecteurs puissants pour la gestion des connaissances, si elles sont utilisees efficacement. Ce livre est une tentative pour presenter une vision quelque peu unifiee de la gestion des connaissances dans une organisation. Cette vision est centree sur la notion de capital de connaissances, en tant que ressource intangible fondamentale propre a l'organisation, qu'il s'agit de gerer au mieux, dans tous les sens du terme. C'est une vision operatoire, qui examine les processus essentiels qui permettent de valoriser, perenniser, faire evoluer ce capital et qui mettent en avant les points ou la connaissance peut apporter de la valeur ajoutee a l'organisation. Le delicat probleme de fond de la nature de la connaissance (individuelle et/ou collective) dans une organisation n'est pas aborde. Il est traite dans un ouvrage precedent , et il apparait en filigrane ici, dans la mesure ou le capital connaissances est considere comme un sous-systeme de l'organisation, ce qui est, a notre avis, l'hypothese fondatrice du concept de " gestion des connaissances ".
Human SPA-1 Gene Product Selectively Expressed in Lymphoid Tissues Is a Specific GTPase-activating Protein for Rap1 and Rap2

Mouse Spa-1 gene with a region homologous to the human rap1GAP gene is transcriptionally induced in the lymphocytes by mitogenic stimulation. Herein we have cloned a cDNA for its human counterpart. SPA-1 cDNA encodes a 130-kDa protein (p130 SPA-1 ) consisting of proline-rich regions and rap1GAP-related domain followed by a coiled-coil stretch. Baculovirally expressed p130 SPA-1 exhibited GTPase-activating protein (GAP) activity for Rap1 and Rap2, but not for Ras, Rho, Cdc42, Rac, and Ran, with comparable specific activity to the rap1GAP gene product (p85/95 rap1GAP ). In the cells, p130 SPA-1 was mostly localized at the perinuclear membranous region co-localizing with Rap1 and Rap2. Expression of SPA-1 and rap1GAP genes tended to be segregate in various tissues, lymphoid tissues expressing abundantSPA-1 transcript without rap1GAP, while those such as brain, kidney, and pancreas exhibiting rap1GAPmRNA with little SPA-1. Promyelocytic HL-60 cells, which expressed p130 SPA-1 with little p85/95 rap1GAP in uninduced state, showed progressive decline in p130 SPA-1 and conversely drastic increase in p85/95 rap1GAP as they ceased from proliferation and differentiated into macrophages by 12-O-tetradecanoylphorbol-13-acetate. These results suggested that products of SPA-1 and rap1GAPgenes, albeit comparable GAP activity for Rap1 and Rap2, functioned in the distinct contexts depending on cell types and/or states.
Autobiographical memory specificity and affect regulation: coping with a negative life event

Two decades of research have shown that depressed patients experience significant difficulties retrieving specific autobiographical memories. Importantly, reduced autobiographical memory (AM) specificity is a known vulnerability factor for depression and is predictive of a more chronic course. One of the models that has been put forward to explain the origin of this reduced specificity is the affect‐regulation model, which assumes that being less specific might help to prevent negative or painful emotions by recalling events in a less specific way. This avoidant memory style might have beneficial effects in the short run (less emotional impact of stressful events) but is detrimental in the long run. The affect‐regulation model, and more in particular the beneficial short‐term effect of reduced memory specificity, was investigated in a prospective study. Students were followed over a period of 9 weeks after they failed at their first exams at university. In line with the affect‐regulation model, memory specificity predicted the course of symptoms that were experienced as a result of failing these exams. The less specific the student, the less durable the emotional distress over this 9‐week period. The correlational nature of this study limits to some extent the conclusions that can be drawn. The results offer support for the affect‐regulation account of reduced autobiographical memory specificity. Depression and Anxiety. © 2007 Wiley‐Liss, Inc.
Inhibition of adenylyl cyclase isoforms V and VI by various Gβγ subunits

An intriguing development in the G‐protein signaling field has been the finding that not only the Gα subunit, but also Gβγ subunits, affect a number of downstream target molecules. One of the downstream targets of Gβγ is adenylyl cyclase, and it has been demonstrated that a number of isoforms of adenylyl cyclase can be either inhibited or stimulated by Gβγ subunits. Until now, adenylyl cyclase type I has been the only isoform reported to be inhibited by free Gβγ. Here we show by transient cotransfection into COS‐7 cells of either adenylyl cyclase V or VI, together with Gγ2 and various Gβ subunits, that these two adenylyl cyclase isozymes are markedly inhibited by Gβγ. In addition, we show that Gβ1 and Gβ5 subunits differ in their activity. Gβ1 transfected alone markedly inhibited adenylyl cylcase V and VI (probably by recruiting endogenous Gγ subunits). On the other hand, Gβ5 produced less inhibition of these isozymes, and its activity was enhanced by the addition of Gγ2. These results demonstrate that adenylyl cyclase types V and VI are inhibited by Gβγ dimers and that Gβ1 and Gβ5 subunits differ in their capacity to regulate these adenylyl cyclase isozymes. —Bayewitch, M. L. , Avidor‐Reiss, T. , Levy, R. , Pfeuffer, T. , Nevo, I. , Simonds, W. F. , Vogel, Z. Inhibition of adenylyl cyclase isoforms V and VI by various Gβγ subunits. FASEB J. 12, 1019–1025 (1998)
Extending Byzantine Fault Tolerance to Replicated Clients

Byzantine agreement protocols for replicated deterministic state machines guarantee that externally requested operations continue to execute correctly even if a bounded number of replicas fail in arbitrary ways. The state machines are passive, with clients responsible for any active ongoing application behavior. However, the clients are unreplicated and outside the fault-tolerance boundary. Consequently, agreement protocols for replicated state machines do not guarantee continued correct execution of long-running client applications. Building on the Castro and Liskov Byzantine Fault Tolerance protocol for unreplicated clients (CLBFT), we present a practical algorithm for Byzantine fault-tolerant execution of long-running distributed applications in which replicated deterministic clients invoke operations on replicated deterministic servers. The algorithm scales well to large replica groups, with roughly double the latency and message count when compared to CLBFT, which supports only unreplicated clients. The algorithm supports both synchronous and asynchronous clients, provides fault isolation between client and server groups with respect to both correctness and performance, and uses a novel architecture that accommodates externally requested software upgrades for long-running evolvable client applications. Type of Report: Other Department of Computer Science & Engineering Washington University in St. Louis Campus Box 1045 St. Louis, MO 63130 ph: (314) 935-6160 Extending Byzantine Fault Tolerance to Replicated Clients Ian Wehrman iwehrman@cse.wustl. edu Sajeeva L. Pallemulle sajeeva@cse.wustl. edu Computer Science and Engineering Washington University in St. Louis Kenneth J. Goldman kjg@cse.wustl. edu
Condition monitoring and diagnostics of motor and stator windings – A review

Condition-based maintenance (also called predictive maintenance) requires diagnostic tools to determine when significant insulation aging has occurred, and thus when maintenance is necessary to avoid an in-service failure. Over the years many off-line diagnostic tests and on-line monitoring systems have been proposed and implemented, especially on critical motors and generators. In the past decade, most of the research has concentrated on improving existing off-line diagnostic tests and on-line monitoring such as magnetic flux, partial discharge, temperature, endwinding vibration, etc. However, some newer tests such as polarization/depolarization current, dielectric spectroscopy and on-line leakage current monitoring have been introduced. These tests and monitoring systems are reviewed. Tests and monitoring systems for rotor winding insulation and stator winding insulation are addressed separately.
Title Efficient Temporal and Spatial Data Recovery Scheme for Stochastic and Incomplete Feedback Data of Cyber-physical Systems

Feedback loss can severely degrade the overall system performance, in addition, it can affect the control and computation of the Cyber-physical Systems (CPS). CPS hold enormous potential for a wide range of emerging applications including stochastic and time-critical traffic patterns. Stochastic data has a randomness in its nature which make a great challenge to maintain the real-time control whenever the data is lost. In this paper, we propose a data recovery scheme, called the Efficient Temporal and Spatial Data Recovery (ETSDR) scheme for stochastic incomplete feedback of CPS. In this scheme, we identify the temporal model based on the traffic patterns and consider the spatial effect of the nearest neighbor. Numerical results reveal that the proposed ETSDR outperforms both the weighted prediction (WP) and the exponentially weighted moving average (EWMA) algorithm regardless of the increment percentage of missing data in terms of the root mean square error, the mean absolute error, and the integral of absolute error. Keywords-cyber-physical system; data recovery scheme; stochastic data; temporal correlation; spatial correlation; auto regressive integrated moving average
Characterization of silicone elastomer vaginal rings containing HIV microbicide TMC120 by Raman spectroscopy

Silicone elastomer vaginal rings are currently being pursued as a controlled‐release strategy for delivering microbicidal substances for the prevention of heterosexual transmission of HIV. Although it is well established that the distribution of drugs in delivery systems influences the release characteristics, in practice the distribution is often difficult to quantify in‐situ. Therefore, the aim of this work was to determine whether Raman spectroscopy might provide a rapid, non‐contact means of measuring the concentrations of the lead candidate HIV microbicide TMC120 in a silicone elastomer reservoir‐type vaginal ring. Vaginal rings loaded with TMC120 were manufactured and sectioned before either Raman mapping an entire ring cross‐section (100 μm resolution) or running line scans at appropriate time intervals up to 30 h after manufacture. The results demonstrated that detectable amounts of TMC120, above the silicone elastomer saturation concentration, could be detected up to 1 mm into the sheath, presumably as a consequence of permeation and subsequent reprecipitation. The extent of permeation was found to be similar in rings manufactured at 25 and 80°C.
Expanding Window Fountain coded scalable video in broadcasting

The Expanding Window Fountain (EWF) codes are a new class of rateless codes, providing Unequal Error Protection (UEP). These codes are especially suitable for Scalable Video Coding (SVC) having unequally important data layers. In this paper, we show that EWF codes are usable for transmitting scalable video in mobile channels. We characterize the channel by field measurements in a DVB-H network. We tune the EWF code parameters to achieve real-time transmissions of SVC video. Moreover, we use a code symbol header for the EWF codes, which is also used in the IPDC over DVB-H standard, such that the codes can be efficiently used without the need for excessive packet header redundancy. We show that if some latency in the video is acceptable, then EWF codes can be used for real-time transmissions.
REPRODUCTIVE ISOLATION BETWEEN DIVERGENT RACES OF PEA APHIDS ON TWO HOSTS. II. SELECTION AGAINST MIGRANTS AND HYBRIDS IN THE PARENTAL ENVIRONMENTS

Abstract Sympatric races of pea aphids on alfalfa and red clover are highly ecologically specialized and significantly reproductively isolated. Much of the restriction of gene flow between the specialized populations is due to habitat choice behavior of the winged colonizers (Via 1999). Here, we document additional pre- and postmating reproductive isolation through selection against migrants and hybrids in the parental environments. First, a group of randomly chosen genotypes from each race that were experimentally migrated between hosts had very low survival and reproduction on the alternate host relative to genotypes originating from that host (natives). Such selection against cross-host migrants forms a premating barrier to gene flow because it is likely to reduce migrant frequencies before the sexual forms are induced in the fall. Our reciprocal transplant experiment also shows that natural selection acts directly on individual migrants between the crops to favor host choice behavior: genotypes from each host suffered large losses of fitness when forced to migrate to the alternate host plant relative to the fitness they would have enjoyed had they been able to choose their native host. In a companion field study, sequential sampling throughout the summer in newly colonized fields of both alfalfa and clover revealed a decrease in the frequency of host-specific marker alleles characteristic of the alternate crop. These field data further support the hypothesis that selection disfavors migrants that cross between crops. Second, when two sets of F1 hybrids between the races were reciprocally tested on alfalfa and clover, both sets had significantly lower average fitness than the specialized parent in each of the two environments. This demographic selection against hybrids in the parental environments is a source of postmating reproductive isolation between the specialized races. Finally, significant genetic variation in fitness traits was seen among F1 hybrid genotypes from both crosses between alfalfa and clover specialists. Although this variation suggests that a generalized pea aphid could evolve, such generalists are not seen in field collections of these populations. Corresponding Editor: C. Boggs
Antiseptics for treating infected wounds: Efficacy on biofilms and effect of pH

Abstract Biofilm recalcitrance is a persistent problem when managing difficult to heal and infected chronic wounds. The wound biofilm is a fundamental factor in the re-occurrence and delayed healing commonly observed in non-healing and infected chronic wounds. However, there is presently no single antimicrobial agent that is completely efficacious against both the planktonic and sessile polymicrobial communities evident in at risk or infected wounds. We will review currently available antimicrobials, with particular emphasis on silver and iodine, employed to help suppress biofilms in wounds. In addition, we will also review the effect of pH on antimicrobial efficacy. Available evidence suggests that it is best to take a multifactorial approach towards controlling biofilm in chronic, “at risk” and infected wounds. This highlights the growing importance of avoiding indiscriminate or inappropriate use of antimicrobials in the treatment of chronic wounds.
Thin Layer Chromatography of KREBS Cycle Acids

A systematic appraisal of a large number of solvent systems was carried out for the separation of lactic, pyruvic and 3-hydroxybutyric acids and all of the KREBS cycle acids, except oxalosuccinic, using silica gel G and cellulose thin layer Chromatographie plates. Four solvent systems which allowed separation of various combinations of acids are described and the limits of this method of separation are detailed.
Autonomous trajectory planning and following for industrial underwater manipulators

While autonomous motion planning and control of ground based manipulators has received a lot of interest by researchers in the past three decades, autonomous operation of industrial underwater manipulators is not that well documented. The main bottlenecks in the autonomous operation of underwater manipulators is the dependance on factory supplied master-slave controllers and absence of precise, high speed computer based controllers. Recently, a computer based communication interface was presented in [1] for the industrial Orion7P manipulator from Schilling robotics. The present paper builds up on this computer interface and presents the application of existing and proven methods of autonomous motion planning and control from ground based manipulation to the underwater manipulation domain. Approaches for autonomous collision free path planning and trajectory following are presented for applications involving industrial standard underwater manipulators. These approaches are validated by experiments conducted on a real Orion7P manipulator. This paper thus presents a complete approach for autonomous collision free trajectory planning and execution for industrial underwater manipulators.
Cross-Layer Strategy for Maximizing Equilibrium Lifetime in Wireless Sensor Networks

For wireless sensor networks (WSNs), which have crucial limitation on energy as all the nodes rely on nonrenewable batteries and are often inconvenient to be replaced or recharged, the energy efficiency that has a close relationship with system longevity becomes a significant issue. In this paper, a cross-layer strategy in terms of physical layer and network layer is proposed for maximizing equilibrium lifetime of WSNs. In the proposed strategy, the residual energy ratio (RER) of individual node is taken into account and regarded as a key factor in power control and routing selection. Utilized to identify the relay capability of an intermediate node, the RER is quantized as a specific access probability, which is highly related to the contention window of each node in the end-to-end transmission link. By way of intelligent and efficient allocation of transmission power, and reasonable routing, the proposed cross-layer tactic can achieve preferable performance with energy efficiency. Simulation results prove that the cross-layer based equilibrium power control and routing (EPCR) strategy could greatly prolong the system lifetime of WSNs.
Determination of the band gap and the split-off band in wurtzite GaAs using Raman and photoluminescence excitation spectroscopy

GaAs nanowires with 100% wurtzite structure are synthesized by the vapor-liquid-solid method in a molecular beam epitaxy system, using gold as a catalyst. We use resonant Raman spectroscopy and photoluminescence to determine the position of the crystal-field split-off band of hexagonal wurtzite GaAs. The temperature dependence of this transition enables us to extract the value at 0 K, which is 1.982 eV. Our photoluminescence excitation spectroscopy measurements are consistent with a band gap of wurtzite GaAs below 1.523 eV.