Miracle-dz/UniFAR-FTUs huggingface dataset:
R-Ketorolac Targets Cdc42 and Rac1 and Alters Ovarian Cancer Cell Behaviors Critical for Invasion and Metastasis

Cdc42 (cell division control protein 42) and Rac1 (Ras-related C3 botulinum toxin substrate 1) are attractive therapeutic targets in ovarian cancer based on established importance in tumor cell migration, adhesion, and invasion. Despite a predicted benefit, targeting GTPases has not yet been translated to clinical practice. We previously established that Cdc42 and constitutively active Rac1b are overexpressed in primary ovarian tumor tissues. Through high-throughput screening and computational shape homology approaches, we identified R-ketorolac as a Cdc42 and Rac1 inhibitor, distinct from the anti-inflammatory, cyclooxygenase inhibitory activity of S-ketorolac. In the present study, we establish R-ketorolac as an allosteric inhibitor of Cdc42 and Rac1. Cell-based assays validate R-ketorolac activity against Cdc42 and Rac1. Studies on immortalized human ovarian adenocarcinoma cells (SKOV3ip) and primary patient-derived ovarian cancer cells show that R-ketorolac is a robust inhibitor of growth factor or serum-dependent Cdc42 and Rac1 activation with a potency and cellular efficacy similar to small-molecule inhibitors of Cdc42 (CID2950007/ML141) and Rac1 (NSC23766). Furthermore, GTPase inhibition by R-ketorolac reduces downstream p21-activated kinases (PAK1/PAK2) effector activation by >80%. Multiple assays of cell behavior using SKOV3ip and primary patient-derived ovarian cancer cells show that R-ketorolac significantly inhibits cell adhesion, migration, and invasion. In summary, we provide evidence for R-ketorolac as a direct inhibitor of Cdc42 and Rac1 that is capable of modulating downstream GTPase-dependent, physiologic responses, which are critical to tumor metastasis. Our findings demonstrate the selective inhibition of Cdc42 and Rac1 GTPases by an FDA-approved drug, racemic ketorolac, that can be used in humans. Mol Cancer Ther; 14(10); 2215–27. ©2015 AACR.
Whole mathematics competences assessment at aerospace engineering sophomore

Mathematics background of Engineering students changes drastically between freshmen and sophomore students. This brings out the way in which lab sessions are incorporated must be taken into account as well in the continuous assessment of the subject being a key issue how to incorporate the assessment of lab sessions. At the Higher Technical School of Design Engineering of the Universität Politècnica of Valencia (UPV) we started using CAS almost 20 years ago and have evolved from the initial lab sessions and their corresponding evaluation. Our learning tells us that the final approach depends on the content of the subject and how relevant are the methods used as well as the capabilities of the CAS used. In addition, the standard use of assignments to reinforce the learning process of each student has also shown to be a keystone to increase the Engineering students competences before each assessment test. Results will be presented concerning a second year Mathematics course in Aerospace Engineering where 100% students attendance was achieved at the end of the third semester, all of them taking all exams, and where we experienced a success rate above 91% which is far of being standard at Spanish Engineering studies.
Heart rate variability in adolescents: relations to physical activity, fitness, and adiposity.

PURPOSE We determined the degree to which variation in cardiac autonomic modulation was explained by race, sex, moderate-vigorous physical activity (MVPA), cardiovascular fitness (CVF), percent body fat (%BF), waist girth, subcutaneous abdominal adipose tissue (SAAT), and visceral adipose tissue (VAT). METHODS Subjects were 304 adolescents; SAAT and VAT values were available for 168 youths. Cardiac parasympathetic modulation (PM) was the root mean square of successive differences (RMSSD). Sympathetic-parasympathetic balance was the ratio of low- to high-frequency power (LFnu:HFnu). MVPA was measured with accelerometry, CVF with a treadmill, %BF with dual-energy x-ray absorptiometry (DXA), and SAAT and VAT with magnetic resonance imaging (MRI). RESULTS Root mean square of successive differences was higher, and LFnu:HFnu was lower, in blacks than in whites. The final regression model revealed positive relations with CVF and MVPA, and a %BF by race by sex interaction, such that higher %BF was associated with lower RMSSD in black females and higher RMSSD in white females. Higher RMSSD was associated with lower VAT; for SAAT, the relationship was negative for blacks and positive for whites. For LFnu:Hfnu, a negative relationship was seen with MVPA and higher waist girth was associated with a higher ratio in blacks, but not in whites. Both higher VAT and SAAT were related to higher LFnu:HFnu. CONCLUSIONS Black youths had a more favorable HRV profile than white youths. After controlling for age, race, and sex, more favorable HRV profiles were associated with more MVPA, better CVF, and less visceral and subcutaneous adiposity. The deleterious impact of higher adiposity was greater in blacks, especially females, than in whites. Enhancement of cardiac autonomic modulation may be a pathway through which physical activity, fitness, and leanness contribute to cardiovascular health early in life.
A Scan-Driven Sort Facility for a Relational Database System

In this paper the design and implementation aspects of an integrated sort package are described which was implemented by the author as a part of an experimental relational database system called System R. The sort facility developed for variable length sort keys and tuples is discussed with regard to its anticipated use in relational database systems. It is considered to be a powerful tool to implement complex relational operations like join and projection and to support the dynamic creation of access paths and the loading and reorganizing of data clustered by field values. The input loop of the sort can be driven by various scans which allow the filtering of qualified tuples according to disjunctive normal forms of simple search arguments. Various aspects of the integration with storage and transaction management, locking and logging components are described. A number of design decisions for the sort facility concerning sorting, peerage, and merging techniques are discussed. Finally, some implementation aspects for sorting variable length keys consisting of a number of distributed fields are pointed out.
The Osteogenic and Tenogenic Differentiation Potential of C3H10T1/2 (Mesenchymal Stem Cell Model) Cultured on PCL/PLA Electrospun Scaffolds in the Absence of Specific Differentiation Medium

The differentiation potential of mesenchymal stem cells (MSC) has been extensively tested on electrospun scaffolds. However, this potential is often assessed with lineage-specific medium, making it difficult to interpret the real contribution of the properties of the scaffold in the cell response. In this study, we analyzed the ability of different polycaprolactone/polylactic acid PCL/PLA electrospun scaffolds (pure or blended compositions, random or aligned fibers, various fiber diameters) to drive MSC towards bone or tendon lineages in the absence of specific differentiation medium. C3H10T1/2 cells (a mesenchymal stem cell model) were cultured on scaffolds for 96 h without differentiation factors. We performed a cross-analysis of the cell–scaffold interactions (spreading, organization, and specific gene expression) with mechanical (elasticity), morphological (porosity, fibers diameter and orientation) and surface (wettability) characterizations of the electrospun fibers. We concluded that (1) osteogenic differentiation can be initiated on pure PCL-based electrospun scaffolds without specific culture conditions; (2) fiber alignment modified cell organization in the short term and (3) PLA added to PCL with an increased fiber diameter encouraged the stem cells towards the tendon lineage without additional tenogenic factors. In summary, the differentiation potential of stem cells on adapted electrospun fibers could be achieved in factor-free medium, making possible future applications in clinically relevant situations.
Ambient Systems , Networks and Technologies ( ANT 2016 ) Pedestrian Warrants For Developing Countries By Simulation Approach

Jay walkers are exposed to higher risk while crossing the road due to lack of appropriate pedestrian warrants and standards. The pedestrian facilities and standards adopted in developed countries are not feasible in developing nation like India. Mid-block pedestrian crossing with different land uses as criteria was selected as case study inside Delhi Urban area. The PTV Vissim and PTV Viswalk, widely used micro simulation software used for modelling heterogeneous, non-lane behavior traffic and pedestrian-vehicular interaction. The inbuilt calibration parameters have been developed considering lane based and homogeneous traffic with less complex characteristics. Hence the calibration parameter for Indian traffic and pedestrian behavior was identified and modified with various combinations for the best representation in the simulation model. As a proposal suitable warrants are developed and simulated for the safe pedestrian movement. © 2016 The Authors. Published by Elsevier B.V. Peer-review under responsibility of the Conference Program Chairs.
Medication reconciliation at patient admission: a randomized controlled trial

Objective: To measure length of hospital stay (LHS) in patients receiving medication reconciliation. Secondary characteristics included analysis of number of preadmission medications, medications prescribed at admission, number of discrepancies, and pharmacists interventions done and accepted by the attending physician. Methods: A 6 month, randomized, controlled trial conducted at a public teaching hospital in southern Brazil. Patients admitted to general wards were randomized to receive usual care or medication reconciliation, performed within the first 72 hours of hospital admission. Results: The randomization process assigned 68 patients to UC and 65 to MR. LHS was 10±15 days in usual care and 9±16 days in medication reconciliation (p=0.620). The total number of discrepancies was 327 in the medication reconciliation group, comprising 52.6% of unintentional discrepancies. Physicians accepted approximately 75.0% of the interventions. Conclusion: These results highlight weakness at patient transition care levels in a public teaching hospital. LHS, the primary outcome, should be further investigated in larger studies. Medication reconciliation was well accepted by physicians and it is a useful tool to find and correct discrepancies, minimizing the risk of adverse drug events and improving patient safety.
Formal Requirements Speci(cid:2)cation for Command and Control Systems

This paper presents an approach to formal require(cid:2) ments speci(cid:3)cation of embedded systems(cid:4) The speci(cid:3)c demands of a speci(cid:3)cation for command and control systems are addressed(cid:4) The proposed method allows various views of a system(cid:5) like conventional methods(cid:4) The added value lies in the fact that the relationship between the views is speci(cid:3)ed formally(cid:5) and consistency between views can be analyzed formally(cid:4) As a case study(cid:5) we develop and analyze a formal requirements speci(cid:3)cation for a subsystem of a realistic command and control system(cid:4) Speci(cid:3)cation
Early diagnosis of low grade malignant lymphoma and chronic lymphocytic leukaemia. Verification of morphologically suspected malignancy in blood lymphocytes by flow cytometry

Abstract: According to international recommendations, the diagnosis of chronic lymphocytic leukaemia (CLL) is made on the grounds of persistent peripheral lymphocytosis or lymphocytic infiltration of the bone marrow. The appearance of morphologically atypical lymphocytes in low‐grade malignant lymphoma (NHL) or CLL is easily overlooked, and is generally not regarded as a diagnostic criterion. We report 12 cases of CLL/NHL who were detected only by morphological screening of routine peripheral blood smears (83,400 blood smears). The diagnosis of CLL/NHL was not suggested by the patients' history, physical or other laboratory findings, and had not been contemplated by the physician in charge. Monoclonality of peripheral lymphocytes was confirmed by immunophenotyping and Southern blotting. The lymphoma was classified histologically according to the International Working Formulation (bone marrow biopsy). A monoclonal lymphatic population was not found by immunophenotyping in the blood of 11 patients whose lymphocytes were morphologically classified as activated‐abnormal. However, 3 patients suspected to have NHL/CLL on morphological screening of blood smears had to be classified as benign after immunophenotyping. We conclude that morphological screening of routine blood smears is an inexpensive and easy additional screening method for CLL and low grade malignant NHL.
The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog.

WHI1‐1 is a dominant mutation that reduces cell volume by allowing cells to commit to division at abnormally small sizes, shortening the G1 phase of the cell cycle. The gene was cloned, and dosage studies indicated that the normal gene activated commitment to division in a dose‐dependent manner, and that the mutant gene had a hyperactive but qualitatively similar function. Mild over‐expression of the mutant gene eliminated G1 phase, apparently entirely relaxing the normal G1 size control, but revealing hitherto cryptic controls. Sequence analysis showed that the hyperactivity of the mutant was caused by the loss of the C‐terminal third of the wild‐type protein. This portion of the protein contained PEST regions, which may be signals for protein degradation. The WHI1 protein had sequence similarity to clam cyclin A, to sea urchin cyclin and to Schizosaccharomyces pombe cdc13, a cyclin homolog. Since cyclins are inducers of mitosis, WHI1 may be a direct regulator of commitment to division. A probable accessory function of the WHI1 activator is to assist recovery from alpha factor arrest; WHI1‐1 mutant cells could not be permanently arrested by pheromone, consistent with a hyperactivation of division.
Integrated MANs with hierarchical architecture

A description is given of metropolitan area networks (MANs) for integrated services that consist of local clusters connected through bridges to a backbone network. The services integration as well as the required flow control functions are obtained with simple procedures which operate at the media access control (MAC) level. The effectiveness of this hierarchical architecture is evaluated in the case where the local clusters and the backbone network are implemented using L-express buses. The efficiency of this architecture is compared with that of other alternatives and detailed performance results in saturation condition, obtained with simulations, are presented. <<ETX>>
Regional Innovation Systems in China: A long-term perspective based on patent data at the prefectural level

This paper focuses on the connections between long-term development and Regional Innovation Systems (RIS) in China. It aims to investigate how the evolution of RIS fits with China’s overall process of economic upgrading. The analysis relies on Chinese patent applications filed to the EPO during the period 1981 to 2009, which authors have regionalised at a prefectural level. Conceptu-ally, the investigation concerns the relative prevalence of indexes derived from inventors’ and ap-plicants’ localisation to describe local innovation activities in terms of emergence, development and reinforcement. The hypothesis ranks higher those prefectures where indigenous applicants prevail, that is, the initiative, organisation and exploitation of innovation activities are foremost local (or endogenous). Results return the possibility of grouping Chinese prefectures into six clusters. On this basis, RIS features appear to diffuse, even while regional concentration of innovation activities is still increasing. This pattern is deemed to fit the process of industrial development in China very well. As it was in the past, RIS benefit from the opportunities that a long-term development strategy provides, but face its limits as well.
Tailing of thermal inactivation curve of Aspergillus niger spores

The nonlinear thermal inactivation of Aspergillus niger spores in phosphate-citrate buffer was studied. The thermal inactivation pattern of the spore consisted of a shoulder, an accelerated decline, and a tail at various constant temperatures around 60 degrees C. The pattern fitted a thermotolerant subpopulation model. In the model, we postulated that some spores in the initial population had become thermotolerant at a certain ratio during heating. The model parameters including the rate coefficients, the time lag, and the existence ratio of thermotolerant cells were analyzed at various temperatures. The tailing was not observed at an initial concentration below 10(3) cells per ml. Cells cultured from thermotolerant cells showed an inactivation pattern similar to that of the original cells. Also, cells at the second heating showed the same thermotolerance as or were slightly more thermosensitive than the original cells. Intermittent heating was found to be effective to inactivate cells at a high concentration.
Beliefs Regarding Stimulant Medication Effects Among College Students With a History of Past or Current Usage

Objective: To examine the beliefs of ADHD college students concerning stimulant medications and to apply the theory of planned behavior toward better understanding the factors instrumental in decisions regarding stimulant use. Method: A cross-sectional, correlational design was used, and students completed a survey under controlled laboratory conditions. Participants were 193 students taking introductory psychology who self-reported receiving a diagnosis of attention deficit disorder or ADHD and a treatment history of using stimulant medications. Results: Beliefs regarding the effects of medication use are represented by four factors ((i.e., improved attention/academics, loss of authentic self, social self-enhancement, and common side effects), where the first three significantly and systematically differentiate between those currently using stimulants and those who are not. Conclusion: To understand decisions regarding stimulant use, it is important to consider how college students perceive the positive and negative effects of the medication with respect to sense of self and social relationships.
A Voluntary Serosurvey and Behavioral Risk Assessment for Human Immunodeficiency Virus Infection Among College Students

The authors conducted a voluntary serosurvey and educational campaign among 3394 undergraduate students attending the University of Maryland at College Park to determine the prevalence of and risk factors for human immunodeficiency virus type 1 (HIV-1) infection. Two students were seropositive (0.06%, 95% confidence interval 0–0.15%). Both were homosexual men with multiple sexual partners. Despite the low prevalence of infection, potential risk factors for transmission of HIV-1 were common, as assessed by a self-administered anonymous questionnaire. These included a previous sexually transmitted disease (12.6%), male homosexual intercourse (4.8% of men), heterosexual anal intercourse (25.3%), heterosexual intercourse with a person at risk (an HIV-1 infected person, a bisexual man, a parenteral drug user, a female prostitute, or a hemophiliac) (5.2%), multiple sexual partners (21% reported 10 or more lifetime partners), and intravenous drug use (1.3%). Assessment of the efficacy of our program by comparing responses on pre- and post-test questionnaires showed gains in knowledge about heterosexual transmission of HIV-1 and an increase in the reported frequency of condom use 1–2 months after participating in the survey. The authors conclude that HIV-1 infections are occurring among college students but in our study group remain confined to persons with known high-risk behavior; however, practices that may support transmission are common, and programs designed to diminish these behaviors among college students are needed.
Targeting induced local lesions IN genomes (TILLING) for plant functional genomics.

One of the most important breakthroughs in the history of genetics was the discovery that mutations can be induced ([Muller, 1930][1]; [Stadler, 1932][2]). The high frequency with which ionizing radiation and certain chemicals can cause genes to mutate made it possible to perform genetic studies
Targeting WISP1 to sensitize esophageal squamous cell carcinoma to irradiation

Radiotherapy is a primary treatment modality for esophageal squamous cell carcinoma (ESCC). However, most of patients benefited little from radiotherapy due to refractory radioresistance. We found that WISP1, a downstream target gene of Wnt/β-catenin pathway, was re-expressed in 67.3 % of ESCC patients as an oncofetal gene. Expression of WISP1 predicted prognosis of ESCC patients treated with radiotherapy. Overall survival in WISP1-positive patients was significantly poorer than in WISP1-negative patients. Serum concentration of WISP1 after radiotherapy reversely correlated with relapse-free survival. Gain and loss of function studies confirmed that WISP1 mediated radioresistance both in esophageal squamous cancer cells and in xenograft tumor models. Further studies revealed that WISP1 contributed to radioresistance primarily by repressing irradiation-induced DNA damage and activating PI3K kinase. LncRNA BOKAS was up-regulated following radiation and promoted WISP1 expression and resultant radioresistance. Furthermore, WISP1 facilitated its own expression in response to radiation, creating a positive feedback loop and increased radioresistance. Our study revealed WISP1 as a potential target to overcome radioresistance in ESCC.
Input to state stability properties of nonlinear systems and applications to bounded feedback stabilization using saturation

The concepts of stability, attractivity and asymptotic stability for systems subject to restrictions of the input values are introduced and analyzed in terms of Lyapunov functions. A comparison with the well known input-to-state stability property introduced by Sontag is provided. We use these concepts in order to derive sufficient conditions for global stabilization for triangular and feedforward systems by means of saturated bounded feedback controllers and also recover some recent results due to Teel.
Knowledge model based approach in recognition of on-line Chinese characters

A knowledge model-based OCR system is presented for the recognition of on-line connected stroke Chinese characters. In the approach, segment attributes are first extracted to characterize the segment sequence of an unknown character. Next, radical recognition based on model matching is adopted as the coarse classification to reduce the number of candidate characters before detailed matching. Finally, a deviation modeling method is proposed to recognize not only regular writing characters but also characters with stroke-order and stroke-number deviations. The effectiveness of the approach is verified by experiments on the recognition of on-line Chinese characters. >
Interactive Connectivity Establishment (ICE): A Protocol for Network Address Translator (NAT) Traversal for Offer/Answer Protocols

This document describes a protocol for Network Address Translator (NAT) traversal for multimedia session signaling protocols based on the offer/answer model, such as the Session Initiation Protocol (SIP). This protocol is called Interactive Connectivity Establishment (ICE). ICE makes use of existing protocols, such as Simple Traversal of UDP Through NAT (STUN) and Traversal Using Relay NAT (TURN). ICE makes use of STUN in peer-to-peer cooperative fashion, allowing participants to discover, create and verify mutual connectivity.