Miracle-dz/UniFAR-FTUs huggingface dataset:
Intake of Omega-3 and Omega-6 Fatty Acids and Risk of Basal and Squamous Cell Carcinomas of the Skin: A Longitudinal Community-Based Study in Australian Adults

Intake of omega-3 and omega-6 fatty acids may modify the risk of basal and squamous cell carcinoma of the skin (BCC and SCC), but population-based evidence is limited and inconsistent. We examined prospectively associations between intake of omega-3 and omega-6 fatty acids estimated from food frequency questionnaires and BCC and SCC incidence among 1322 randomly selected adults in Nambour, Australia. Relative risks (RR) and 95% confidence intervals (CI) were estimated based on histologically confirmed tumors diagnosed between 1997 and 2007. Incidence of BCC was lowest in the middle third of both total omega-6 intake (RRmv.adj = 0.74, 95% CI = 0.56–0.97) and linoleic acid intake (RRmv.adj = 0.75, 95% CI = 0.57–0.99) compared with the lowest third of intake. Evidence for associations with SCC was weak, though persons with arachidonic acid intake in the middle third had a marginally increased risk of SCC (RRmv.adj = 1.42, 95% CI = 1.00–2.02). Consumption of omega-3 fatty acids was not associated with subsequent skin cancer risk. Suggestion that intake of arachidonic acid may be associated with increased SCC incidence and total omega-6 with reduced BCC from our study is still highly uncertain and may be due to chance. These data do not support an association between these fatty acids and risk of BCC or SCC.
Treating complexity: Collaborative care for multiple chronic conditions

Abstract Individuals with co-morbid chronic medical illness and psychiatric illness are a costly and complex patient population, at high risk for poor outcomes. Health-risk behaviours (e.g. smoking, poor diet, and sedentary lifestyle), side effects from psychiatric medications, and poor quality medical care all contribute to poor outcomes. Individuals with major depression die, on average, 5 to 10 years before their age-matched counterparts. For individuals with severe mental illness such as bipolar disorder or schizophrenia, life expectancy may be up to 20 years shorter. As the majority of this premature mortality is due to cardiovascular disease, there is a critical need to engage these individuals around the care of chronic medical illness.
Effect of total knee arthroplasty on disease activity in patients with established rheumatoid arthritis: 3-year follow-up results of combined medical therapy and surgical intervention

Though excellent clinical results have been reported for total knee arthroplasty (TKA) in rheumatoid arthritis (RA) patients, the medium-term effect of TKA on RA disease activity remains unknown. This analysis aimed to assess changes in disease activity after TKA in patients with established RA. We analyzed the systemic effects of TKA on RA disease activity 3 years after intervention. Routine clinical and laboratory assessments were recorded at baseline, less than less than 0.5 years after TKA, and 3 years after TKA. Of the registered RA patients, 130 TKA patients were followed for 3 years after surgery. RA disease activity was measured using the Disease Activity Score 28 (DAS28). Patients were divided into three groups by preoperative baseline DAS28: low (DAS28 ≤ 3.2, n = 8), moderate (DAS28 > 3.2 but ≤5.1, n = 68), and high (DAS28 > 5.1, n = 54) disease activity. The postoperative DAS28 (<0.5 years [DAS1] and 3 years [DAS3] after surgery) scores of each patient were compared to their baseline (DAS0) scores using the paired t-test. The mean DAS28 decreased from 4.85 (DAS0) to 4.14 (DAS1; P = 1.07E-12), and this decrease was sustained at 3 years (DAS3 = 3.97; P = 4.73E-15). Subanalysis results revealed a systemic effect of TKA on disease activity in patients with moderate or high disease activity (DAS0 = 4.33; DAS1 = 3.72 [P = 5.94E-06]; DAS3 = 3.81 [P = 7.89E-06]; and DAS0 = 5.79; DAS1 = 4.86 [P = 1.14E-08]; DAS3 = 4.37 [P = 1.03E-11], respectively). While no significant changes in medication were noted, the average dose of prednisolone tended to decrease over time. We conclude that TKA, which is known to result in good clinical outcomes for damaged knees, has a secondary systemic effect on RA disease activity. Combination therapy consisting of medical treatment and surgical intervention is thought to effectively improve the condition of RA patients who have destructive arthritis in the knee joint, with the effect lasting for at least 3 years.
Fluvoxamine pharmacokinetics in healthy elderly subjects and elderly patients with chronic heart failure.

AIMS To investigate the effects of age and chronic heart failure (CHF) on the oral disposition kinetics of fluvoxamine. METHODS A single fluvoxamine dose (50 mg) was administered orally to 10 healthy young adults, 10 healthy elderly subjects and 10 elderly patients with CHF. Fluvoxamine concentration in plasma was measured for up to 96 h. RESULTS With the exception of apparent distribution volume, ageing modified all main pharmacokinetic parameters of fluvoxamine. Thus, peak concentration was about doubled {31 +/- 19 vs. 15 +/- 9 ng ml(-1); difference [95% confidence interval (CI)] 16 (3, 29), P < 0.05}, and area under the concentration-time curve was almost three times higher [885 +/- 560 vs. 304 +/- 84 ng h ml(-1); difference (95% CI) 581 (205, 957), P < 0.05]; half-life was prolonged by 63% [21.1 +/- 6.2 vs. 12.9 +/- 6.4 h; difference (95% CI) 8.2 (2.3, 14.1), P < 0.01], and oral clearance was halved (1.12 +/- 0.77 vs. 2.25 +/- 0.66 l h(-1) kg(-1); difference (95% CI) -1.13 (-1.80, -0.46), P < 0.001]. A significant inverse correlation was consistently observed between age and oral clearance (r=-0.67; P < 0.001). The coexistence of CHF had no significant effect on any pharmacokinetic parameters in elderly subjects. CONCLUSIONS Ageing results in considerable impairment of fluvoxamine disposition, whereas CHF causes no significant modifications. Therefore, adjustment of initial dose and subsequent dose titrations may be required in elderly subjects, whereas no further dose reduction is necessary in elderly patients with CHF.
Characterization of the Type 3 Fimbrial Adhesins ofKlebsiella Strains

ABSTRACT The Klebsiella pneumoniae fimbrial adhesin, MrkD, mediates adherence to the basolateral surfaces of renal and pulmonary epithelia and to the basement membranes of tissues. Although all isolates possessing the MrkD adhesin mediate the agglutination, in vitro, of erythrocytes treated with tannic acid, the mrkDgene is not conserved within species. The ability of a plasmid-bornemrkD gene product to mediate binding to type V collagen is associated frequently with strains of K. oxytoca and rarely with strains of K. pneumoniae. In K. pneumoniae, the MrkD adhesin is located within a chromosomally borne gene cluster and mediates binding to collagen types IV and V. The plasmid-borne determinant, mrkD1P, and the chromosomally borne gene, mrkD1C, are not genetically related. Some strains of enterobacteria possess amrkD1C allele that is associated with hemagglutinating activity but does not bind to either type IV or type V collagen.
Baseline comorbidity in kidney transplant recipients: a comparison of comorbidity indices.

BACKGROUND An increasing number of patients starting renal replacement therapy are older and have complex comorbidity. In keeping with these demographics, an increased number of older patients undergo transplantation each year. To date, no study has reported baseline comorbidity characteristics of those who underwent transplantation, validated the use of comorbidity indices, or asked whether comorbidity predicts patient outcome after kidney transplantation. Our objective is to report baseline comorbidity and compare the use of different indices for recipients of kidneys from both deceased and living donors. METHODS Using data from the Canadian Organ Replacement Registry, we tested the ability of 4 comorbidity indices to predict patient survival by using a Cox regression model. Model covariates included donor source, age, race, sex, treatment period, primary renal disease cause, months on dialysis therapy, and comorbidities. RESULTS A total of 6,324 patients were included; 22% had > or =1 comorbid condition at baseline. After adjustment for age, sex, and cause of renal disease, increased comorbidity was associated strongly with reduced patient survival. Of all comorbidity indices examined, the model containing the Charlson Comorbidity Index (CCI) offered the best fit. The model containing log--CCI had an index of concordance of 74%. CONCLUSION The CCI is a suitable tool for the measurement of comorbidity in renal transplant recipients.
The complex SNP and CNV genetic architecture of the increased risk of congenital heart defects in Down syndrome

Congenital heart defect (CHD) occurs in 40% of Down syndrome (DS) cases. While carrying three copies of chromosome 21 increases the risk for CHD, trisomy 21 itself is not sufficient to cause CHD. Thus, additional genetic variation and/or environmental factors could contribute to the CHD risk. Here we report genomic variations that in concert with trisomy 21, determine the risk for CHD in DS. This case-control GWAS includes 187 DS with CHD (AVSD = 69, ASD = 53, VSD = 65) as cases, and 151 DS without CHD as controls. Chromosome 21–specific association studies revealed rs2832616 and rs1943950 as CHD risk alleles (adjusted genotypic P-values <0.05). These signals were confirmed in a replication cohort of 92 DS-CHD cases and 80 DS-without CHD (nominal P-value 0.0022). Furthermore, CNV analyses using a customized chromosome 21 aCGH of 135K probes in 55 DS-AVSD and 53 DS-without CHD revealed three CNV regions associated with AVSD risk (FDR ≤ 0.05). Two of these regions that are located within the previously identified CHD region on chromosome 21 were further confirmed in a replication study of 49 DS-AVSD and 45 DS- without CHD (FDR ≤ 0.05). One of these CNVs maps near the RIPK4 gene, and the second includes the ZBTB21 (previously ZNF295) gene, highlighting the potential role of these genes in the pathogenesis of CHD in DS. We propose that the genetic architecture of the CHD risk of DS is complex and includes trisomy 21, and SNP and CNV variations in chromosome 21. In addition, a yet-unidentified genetic variation in the rest of the genome may contribute to this complex genetic architecture.
Quantitative assessment of spasticity in human T-cell lymphotropic virus type I-associated myelopathy/tropical spastic paraparesis

People with human T-cell lymphotropic virus type I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) develop spasticity. The authors examined 34 patients with HAM/TSP in Perú using a device that measures tone in the gastroc-soleus-Achilles tendon unit and provides a quantitative spasticity assessment (QSA). Tone in the 34 patients was more than double that of women with asymptomatic HTLV-I infection. The device may help to track progression in HTLV-I infection.
Migration of imaginary roots of multiplicity three and four under small deviation of two delays in time-delay systems

This paper studies the migration pattern of characteristic imaginary roots of multiplicity three and four in time-delay systems with two delays when the delay parameters undergo small deviations. Stability analysis for such problems is often based on Puiseux series, as multiple roots are not differentiable with respect to delay parameters. However, in this paper the approach is more traditional without using Puiseux series. In the case of triple roots, we show that the stability crossing curves are smooth; when a perturbation occurs in the delay parameter space, two roots move to one half-plane and one root to the other half-plane. The case of quadruple root is more complicated as the stability crossing curve has a cusp. Thus, in the neighbourhood of the critical point, the delay parameter space is divided in an S-sector and a G-sector. When the parameters move into the G-sector, two roots move to the right half-plane, and the other two roots move to the left half-plane. When the parameters move into the S-sector, then three of the roots move to one half-plane, and the remaining root moves to the other half-plane, depending on the conditions.
An examination of different fetal specific antibodies and magnetic activated cell sorting for the enrichment of fetal erythroblasts from maternal blood

ABSTRACT The aim of the present study was to compare the rates of fetal cells obtained after separation from maternal blood by magnetic activated cell sorting (MACS) using different fetal specific antibodies, and to evaluate the potential role of this method in the prenatal diagnosis of fetal trisomies. Peripheral blood samples were obtained from 42 women carrying chromosomally normal fetuses and from 4 women with aneuploid fetuses (2 cases of 47,XX,+18 and 2 of 47,XY,+21) at 9–20 weeks of gestation. After fetal cells were enriched by MACS with three different monoclonal antibodies (GPA, CD71, CD14), fluorescence in situ hybridization (FISH) with chromosome X, and Y‐specific probes was performed to detect the rates of fetal cells in the samples sorted. FISH with chromosome 13‐, 18‐, and 21‐specific probes was carried out to compare proportions of cells with three‐signal nuclei in chromosomally normal and abnormal groups. In male infants, X‐and Y‐positive cells were detected in 80%, 73.3%, and 66.6% of samples after the separation by antibodies CD14, GPA, and CD71, respectively. The percentage of nuclei with three signals was increased in pregnancies with trisomy, ranging between 2% and 5.18%. Pregnancies with normal fetuses showed 0 to 3.7% of nuclei with three signals. The data demonstrate that fetal cell detection varies depending on the antibodies used for cell sorting. This study provides further evidence on the feasibility of screening for fetal chromosomal abnormalities by enriching maternal blood for fetal cells and using FISH.
About locoregional recurrence risk after lipofilling in breast cancer patients: our experience.

AIM Lipofilling is a part of the clinical practice for breast reshaping in patients who underwent surgery for breast cancer. A strong debate in the Literature is open about a higher risk of cancer relapse caused by growth factors produced by adipocyte stem cells after their engraftment in the recipient tissue. Nowadays there is no agreement over the use of autologous fat tissue for the correction of aesthetic defects following breast conservative surgery. METHODS We have considered 151 patients who underwent a breast conservative surgery in the period April 2004-April 2009, followed by one or more lipofilling sessions in the period June 2006-August 2012. A careful pre-operative evaluation of the tumor characteristics has been made, through imaging exams, MRI included. An intraoperative evaluation of the tumor dimensions and its distance from the surgical edges has also been made, followed by a microscopic analysis through a shaving technique. Lipofilling has been offered to all the patients on average 24 months after surgery. RESULTS We have found no cancer relapses after a mean follow up of 45 months (17-76) after lipofilling and of 69 (27-100) months after surgery. CONCLUSIONS Considering oncologic surgery, we can affirm that lipofilling is safe for the risk of cancer relapses, if performed using all the parameters included in our clinical protocol.
The recognition of facial affect in autistic and schizophrenic subjects and their first-degree relatives

Background. Autism and schizophrenia are considered to be substantially influenced by genetic factors. The endophenotype of both disorders probably also includes deficits in affect perception. The objective of this study was to examine the capacity to detect facially expressed emotion in autistic and schizophrenic subjects, their parents and siblings. Method. Thirty-five subjects with autism and 102 of their relatives, 21 schizophrenic subjects and 46 relatives from simplex (one child affected) and multiplex (more than one child affected) families, as well as an unaffected control sample consisting of 22 probands completed a 50-item computer-based test to assess the ability to recognize basic emotions. Results. The autistic subjects showed a poorer performance on the facial recognition test than did the schizophrenic and the unaffected individuals. In addition, there was a tendency for subjects from multiplex families with autistic loading to score lower on the test than individuals from simplex families with autistic loading. Schizophrenic subjects and their relatives as well as siblings and parents of autistic subjects did not differ from the sample of unaffected subjects in their ability to judge facial affect. Conclusions. Findings corroborate the assumption that emotion detection deficits are part of the endophenotype of autism. In families with autistic children, the extent of facial recognition deficits probably indexes an elevation in familial burden. It seems unlikely that problems in emotion perception form a consistent part of the endophenotype of schizophrenia or the broader phenotype in relatives of patients with psychosis or autism.
Co-Seismic Deformation Due To The Tohoku-oki Earthquake Measured By ENVISAT-ASAR Data And GPS

On March 11, 2011, 05:46 UTC, a giant earthquake of magnitude Mw=9.0 occurred off the Pacific coast of Tohoku, Honshu Island, Japan. Massive damage has been reported, mainly related to the subsequent tsunami. In this work we present first results concerning the displacement induced by the Tohoku-oki earthquake by using ASAR ENVISAT data acquired few days before and after the event. The work points out the reliability of ASAR ENVISAT data to provide interferometricbased displacement field even though the satellite is recently came into a new operative phase with degraded orbital control and SAR data were processed in an emergency framework soon after the acquisition without precise orbital records. After removing artefacts due to strong orbital errors, the interferometric deformation pattern is mainly related to the coseismic displacement and it results comparable to that provided by the GEONET GPS network which has an unique density of in situ measurements found elsewhere in the world.
G‐protein control of voltage dependence as well as gating of muscarinic metabotropic channels in guinea‐pig ileum.

1. Voltage‐dependent properties of muscarinic receptor cationic current activated by carbachol in single smooth muscle cells have been studied using patch‐clamp recording techniques. Cells were obtained by enzymic digestion from the longitudinal muscle layer of guinea‐pig small intestine. 2. The inward cationic current showed a pronounced U‐shaped current‐voltage relationship (inward current negative). The relationship of cationic conductance to voltage could be described by a Boltzman distribution which was shifted 36 mV in the negative direction on the voltage axis by increasing fractional receptor occupancy (by increasing agonist concentration from 3 to 300 microM), and in the positive direction by desensitization during prolonged application of agonist. Cationic channels opened by low and high concentrations of carbachol at the same potential do not have identical properties. 3. Release of GTP within the cell, by flash photolysis of an inert caged precursor, had the same effect on the current‐voltage relationship as increasing receptor occupancy by the agonist. Release of GDP beta S by flash photolysis had the opposite effect. 4. These various results could be explained if cationic channel opening upon receptor activation required binding of at least one alpha‐GTP subunit, but the position of the activation curve on the voltage axis depended critically on the concentration of activated G‐protein alpha‐subunits in the cell.
Motivational patterns of alcohol dependent patients: a replication.

This study attempted to replicate the results of F. W. Willoughby and J. F. Edens (1996), who identified motivational subtypes of alcohol-dependent veterans on the basis of the University of Rhode Island Change Assessment Scale (McConnaughy, Prochaska, & Velicer, 1983). Using cluster analysis, 2 subgroups were delineated that corresponded to the precontemplation (n = 63) and contemplation-action (n = 99) stages of change. Theoretically consistent differences on various dependent (i.e., nonclustering) variables were also replicated. Patients in the precontemplation cluster reported being less concerned over their drinking and less receptive to help, and they acknowledged fewer mood-enhancing benefits of alcohol use. Unlike prior findings, motivational profiles were also associated with treatment outcome, with precontemplation-stage patients being less likely to complete treatment successfully.
MLPA and cDNA analysis improves COL4A5 mutation detection in X‐linked Alport syndrome

The X‐linked form of Alport syndrome (AS) is caused by mutations in the COL4A5 gene encoding the α5 chain of type IV collagen. Most COL4A5 mutations are individual, and mutation analysis is complicated by the size of the gene and the number of exons. Larger structural rearrangements account for 10–15% of mutations. We have established a method for mutation analysis of COL4A5 based on reverse transcriptase‐polymerase chain reaction analysis of mRNA from cultured skin fibroblasts and multiplex ligation‐dependent probe amplification (MLPA) on genomic DNA. One advantage of using skin biopsies for the mRNA analysis is the possibility of immunohistochemical staining for the α5(IV) chain on skin sections to support a diagnosis of X‐linked AS. A mutation was detected in all five cases included. One patient presenting with AS and diffuse leiomyomatosis was found to have a COL4A5 deletion extending into and comprising COL4A6 exons 1, 1′, and 2. We have evaluated the MLPA assay on samples from 67 previously tested AS patients (45 males and 22 females) and 20 controls. We found that the combination of cDNA and MLPA analysis improves the mutation detection rate in COL4A5 and that MLPA should be the first step in genetic testing for X‐linked AS.
Offshore Metallic Platforms Observation Using Dual-Polarimetric TS-X/TD-X Satellite Imagery: A Case Study in the Gulf of Mexico

Satellite-based synthetic aperture radar (SAR) has been proven to be an effective tool for ship monitoring. Offshore platforms monitoring is a key topic for both safety and security of the maritime domain. However, the scientific literature oriented to the observation of offshore platforms using SAR imagery is very limited. This study is mostly focused on the analysis and understanding of the multipolarization behavior of platforms’ backscattering using dual-polarization X-band SAR imagery. This study is motivated by the fact that under low incidence angle and moderate wind conditions, copolarized channels may fail in detecting offshore platforms even when fine-resolution imagery is considered. This behavior has been observed on both medium- and high-resolution TerraSAR-X/TanDEM-X SAR imagery, despite the fact that platforms consist of large metallic structures. Hence, a simple multipolarization model is proposed to analyze the platform backscattering. Model predictions are verified on TerraSAR-X/TanDEM-X SAR imagery, showing that for acquisitions under low incidence angle, the platforms result in a reduced copolarized backscattered intensity even when fine resolution imagery is considered. Finally, several solutions to tackle this issue are proposed with concluding remark that the performance of offshore observation.
The Aging Driver Medicine, Policy, and Ethics

With the graying of America, more older persons will be driving. Physiological changes associated with normal aging and diseases that commonly affect the elderly may compromise their ability to drive safely. Although all states have regulations governing driving licensure, few offer specific guidelines regarding older persons. Accordingly, much of the responsibility for determining medical competence to drive and counseling patients in this regard is left to physicians.