Miracle-dz/UniFAR-FTUs huggingface dataset:
circRNA_0046366 inhibits hepatocellular steatosis by normalization of PPAR signaling

AIM To investigate micro (mi)R-34a-antagonizing circular (circ)RNA that underlies hepatocellular steatosis. METHODS The effect of circRNA on miR-34a was recognized by the miRNA response element (MRE), and validated by the dual-luciferase reporter assay. Its association with hepatocellular steatosis was investigated in HepG2-based hepatocellular steatosis induced by free fatty acids (FFAs; 2:1 oleate:palmitate) stimulation. After normalization of the steatosis-related circRNA by expression vector, analysis of miR-34a activity, peroxisome proliferator-activated receptor (PPAR)α level, and expression of downstream genes were carried out so as to reveal its impact on the miR-34a/PPARα regulatory system. Both triglyceride (TG) assessment and cytopathological manifestations uncovered the role of circRNA in miR-34a-dependent hepatosteatogenesis. RESULTS Bioinformatic and functional analysis verified circRNA_0046366 to antagonize the activity of miR-34a via MRE-based complementation. In contrast to its lowered level during FFA-induced hepatocellular steatosis, circRNA_0046366 up-regulation abolished the miR-34a-dependent inhibition of PPARα that played a critical role in metabolic signaling pathways. PPARα restoration exerted transcriptional improvement to multiple genes responsible for lipid metabolism. TG-specific lipolytic genes [carnitine palmitoyltransferase 1A (CPT1A) and solute-carrier family 27A (SLC27A)] among these showed significant increase in their expression levels. The circRNA_0046366-related rebalancing of lipid homeostasis led to dramatic reduction of TG content, and resulted in the ameliorated phenotype of hepatocellular steatosis. CONCLUSION Dysregulation of circRNA_0046366/miR-34a/PPARα signaling may be a novel epigenetic mechanism underlying hepatocellular steatosis. circRNA_0046366 serves as a potential target for the treatment of hepatic steatosis.
Analyzing AR Viewing Experience through Analytics Heat Maps for Augmented Content

Analytics is a well-known form of capturing information about the user behavior of an application. Augmented reality applications deal with specific data such as the camera pose, not being supported by popular analytics frameworks. To fill such gap, this work proposes an analytics framework solution for augmented reality applications. It supports both marker-based and markerless augmented reality scenarios, collecting data related to camera pose and time spent by the user on each position. Besides the multiplatform capture tool, the framework provides a data analysis visualization tool capable of highlighting the most visited 3D positions, users main areas of interest over the marker plane, the 3D path performed by the camera and also a recovery of the content viewed by the user based on the collected camera pose information.
On-line adaptation of semantic models for spoken language understanding

Spoken language understanding (SLU) systems extract semantic information from speech signals, which is usually mapped onto concept sequences. The distribution of concepts in dialogues are usually sparse. Therefore, general models may fail to model the concept distribution for a dialogue and semantic models can benefit from adaptation. In this paper, we present an instance-based approach for on-line adaptation of semantic models. We show that we can improve the performance of an SLU system on an utterance, by retrieving relevant instances from the training data and using them for on-line adapting the semantic models. The instance-based adaptation scheme uses two different similarity metrics edit distance and n-gram match score on three different to-kenizations; word-concept pairs, words, and concepts. We have achieved a significant improvement (6% relative) in the understanding performance by conducting rescoring experiments on the n-best lists that the SLU outputs. We have also applied a two-level adaptation scheme, where adaptation is first applied to the automatic speech recognizer (ASR) and then to the SLU.
A monolithically integrated 120-GHz InGaAs/InAlAs/InP HEMT amplifier

We report the design and gain performance for a two-stage 120-GHz monolithically integrated amplifier using 0.1-/spl mu/m gate length pseudomorphic In/sub 0.60/Ga/sub 0.40/As/In/sub 0.52/Al/sub 0.48/As/InP HEMT's. The two-stage amplifier demonstrated 10-12 dB gain measured between 119.5 to 123.5 GHz. To the best of our knowledge, this is the first demonstration of a monolithically integrated InP HEMT amplifier reported at D-band. <<ETX>>
Size and shape of receptive fields in the medial superior temporal area (MST) of the macaque

NINETY-ONE single units were recorded in area MSTd of anesthetized and paralyzed macaques. Receptive fields (RFs) were mapped quantitatively using small patches of moving random dots in 25 different positions (the two-dimensional position test, or P2D). The dimensions of the receptive fields (RFs) were estimated by fitting P2D data with a generalized Gaussian function. The half-height areas of the RFs in MSTd were found to average 1085 deg2 and were not dependent upon eccentricity, in contrast to those in MT/V5 (n = 295) which averaged 31 deg2 at the fovea but at the periphery approached the RFs of MSTd in size. The RFs of some MSTd neurons extended 30–40° into the ipsilateral hemifield. In comparison, the overlap was only 10–15° in area MT/V5.
Breast and Cervical Cancer Screening Among Rural Midwestern Latina Migrant and Seasonal Farmworkers

Background: While cancer control and prevention efforts are well documented, limited information on this topic exists for Latina farmworkers in the rural Midwest. This study sought to examine correlates of breast cancer and cervical cancer screening practices of English- and Spanish-speaking Latina farmworkers in Michigan. Methods: Survey and anthropometric data were collected from a community-based cross-sectional sample of 173 Latina agricultural laborers in Michigan. Psychosocial-cultural and socioeconomic variables were examined as predictors of mammography and Papanicolaou screening. Findings: Results showed that individual characteristics that were significantly associated with having a Papanicolaou examination in the last 12 months included having higher language-based acculturation (odds ratio = 3.81), having ever done a breast self-examination (odds ratio = 2.82), and having health insurance (odds ratio = 5.58). Conclusions: Acculturation, insurance, and performance of breast self-examination were key correlates of recent cervical cancer screening among Midwest Latina farmworkers. Findings suggest that education and targeted outreach strategies for Spanish-speaking Latina farmworker women in rural settings are urgently needed.
Mapping Tourism Stakeholders’ Weather and Climate Information-Seeking Behavior in Fiji

AbstractTourism is inherently dependent on weather and climate, and its sustainability and resilience to adverse weather and climate impacts is greatly enhanced by providing tailored climate services to tourism sector stakeholders. Climate services need to integrate standard weather forecasts, with early warning systems, seasonal forecasts, and long-term projections of climatic changes in order to meet the information needs of the sector. While a growing number of studies address the potential climate change impacts on tourism, little is known about how the tourism sector accesses, uses, and analyses the available weather and climate information.This research presents findings from an exploratory study on weather and climate information-seeking behavior of 15 private and public tourism sector stakeholders in the Republic of Fiji. The results show a variety of weather and climate information-seeking paths in use, which differ depending on levels of professional responsibility, weather and climate literacy,.
A componential analysis of the ERP elicited by novel events using a dense electrode array.

In this study, we examined the relationship between the novelty P3 and the P300 components of the brain event-related potential (ERP). Fifteen subjects responded manually to the rare stimuli embedded either in a classical auditory oddball series or in a series in which "novel" stimuli were inserted. The electroencephalogram (EEG) was recorded with a dense array of 129 electrodes. The data were analyzed by using spatial Principal Components Analysis (PCA) to identify a set of orthogonal scalp distributions, "virtual electrodes" that account for the spatial variance. The data were then expressed as ERPs measured at each of the virtual electrodes. These ERPs were analyzed using temporal PCA, yielding a set of "virtual epochs. " Most of the temporal variance of the rare events was associated with a virtual electrode with a posterior topography, that is, with a classical P300, which was active during the virtual epoch associated with the P300. The novel stimuli were found to elicit both a classical P300 and a component focused on a virtual electrode with a frontal topography. We propose that the term Novelty P3 should be restricted to this frontal component.
Development of Mother’s Lifestyle Scale during Pregnancy with an Approach to Social Determinants of Health

Background and Objective: The present study was conducted to design and measure psychometrics of mothers’ lifestyle scale during pregnancy with regards to Iranians’ cultural norms and an approach to social determinants. Method: this study, started by reviewing previous studies and exploring similar questionnaires that examine different domains of lifestyle (nutrition, exercising, self-care, smoking, using alcohol and illegal drugs, social relations, and stress control), then besides translating questions of the questionnaires, content of some questions was modified and proper statements with regards to social determinant of health and Iranian cultural, was used. Secondly, the validity of the designed instruments was determined using face, content, criterion, and construct validity. Thirdly, the reliability of the measurement instruments was examined using Cronbach’s alpha. Participants were Healthy Iranian pregnant women (37-42 week) who refer to selected hospital for delivery. Results: In the first step of the study, of the 222 questions obtained from a review of the related instruments, certain questions were omitted due to their irrelevance to social determinants of health and finally 160 questions were selected in 10 sections. After determining the face and content validity qualitatively and quantitatively and exploratory factor analysis, the number of questionnaire items was reduced to 132. Calculation of Cronbach’s alpha confirmed the high internal consistency (0.76) of the questionnaire. Conclusion: This measurement instrument was designed in the context of the Iranian culture and sounded suitable for studying the pregnant women’s lifestyle due to its appropriate validity and reliability, simplicity, and functionality in different situations.
A model to optimise the requirements of lactating dairy cows for physically effective neutral detergent fibre

This study modelled multiple physiological responses of dairy cows to physical and chemical characteristics of a diet aiming to optimise their requirements for physically effective neutral detergent fibre, expressed inclusive of particles-dry matter > 8 mm (peNDF>8). Extensive research data, comprising a wide range of feeding conditions (n = 64 studies and 257 different dietary treatments), were used to parameterise the model, while statistical modelling was used to account for the inter- and intra-experiment variation as well as to derive the model estimates. Physiological thresholds and ‘safety limits’ of peNDF>8 for maintaining different physiological variables were derived using non-linear statistical modelling. Results showed that peNDF>8 content in the diet is a key factor stimulating rumination activity, maintaining optimal ruminal pH and promoting fibre digestion. Modelling data with regard to the association of fibre digestion and time duration of ruminal pH < 5.8 and dietary peNDF>8 suggests that feeding of less than 13.7% peNDF>8 (the lower ‘safety limit’) is critical to prevent depression of fibre digestion in dairy cows. The study also indicated that the beneficial effects of peNDF>8 on ruminal pH and fibre digestion can be at the expense of the dry matter intake (DMI) level of high-producing cows when the peNDF>8 threshold of 14.9% in the diet is exceeded. In terms of the optimisation of peNDF>8 requirements, the modelling data suggest that feeding of 17–18.5% peNDF>8 can be beneficial in maintaining ruminal pH, while allowing a relatively high DMI (22.3–22.7 kg · d−1) for average high-producing dairy cows.
Zika beyond the Americas: Travelers as sentinels of Zika virus transmission. A GeoSentinel analysis, 2012 to 2016.

Background Zika virus (ZIKV) was first isolated in Africa; decades later, caused large outbreaks in the Pacific, and is considered endemic in Asia. We aim to describe ZIKV disease epidemiology outside the Americas, the importance of travelers as sentinels of disease transmission, and discrepancies in travel advisories from major international health organizations. Methods and findings This descriptive analysis using GeoSentinel Surveillance Network records involves sixty-four travel and tropical medicine clinics in 29 countries. Ill returned travelers with a confirmed or probable diagnosis of ZIKV disease acquired in Africa, Asia and the Pacific seen between 1 January 2012 and 31 December 2016 are included, and the frequencies of demographic, trip, and diagnostic characteristics described. ZIKV was acquired in Asia (18), the Pacific (10) and Africa (1). For five countries (Indonesia, Philippines, Thailand, Vietnam, Cameroon), GeoSentinel patients were sentinel markers of recent Zika activity. Additionally, the first confirmed ZIKV infection acquired in Kiribati was reported to GeoSentinel (2015), and a probable case was reported from Timor Leste (April 2016), representing the only case known to date. Review of Zika situation updates from major international health authorities for country risk classifications shows heterogeneity in ZIKV country travel advisories. Conclusions Travelers are integral to the global spread of ZIKV, serving as sentinel markers of disease activity. Although GeoSentinel data are collected by specialized clinics and do not capture all imported cases, we show that surveillance of imported infections by returned travelers augments local surveillance system data regarding ZIKV epidemiology and can assist with risk categorization by international authorities. However, travel advisories are variable due to risk uncertainties.
Perspectives on Assembling Coronavirus Spikes on Fiber Optics to Reveal Broadly Recognizing Antibodies and Generate a Universal Coronavirus Detector

In time of COVID-19 biological detection technologies are of crucial relevance. We propose here the use of state of the art optical fiber biosensors to address two aspects of the fight against SARS-CoV-2 and other pandemic human coronaviruses (HCoVs). Fiber optic biosensors functionalized with HCoV spikes could be used to discover broadly neutralizing antibodies (bnAbs) effective against known HCoVs (SARS-CoV, MERS-CoV and SARS-CoV-2) and likely future ones. In turn, identified bnAbs, once immobilized onto fiber optic biosensors, should be capable to detect HCoVs as diagnostic and environmental sensing devices. The therapeutic and preventative value of bnAbs is immense as they can be used for passive immunization and for the educated development of a universal vaccine (active immunization). Hence, HCoV bnAbs represent an extremely important resource for future preparedness against coronavirus-borne pandemics. Furthermore, the assembly of bnAb-based biosensors constitutes an innovative approach to counteract public health threats, as it bears diagnostic competence additional to environmental detection of a range of pandemic strains. This concept can be extended to different pandemic viruses, as well as bio-warfare threats that entail existing, emerging and extinct viruses (e.g., the smallpox-causing Variola virus). We report here the forefront fiber optic biosensor technology that could be implemented to achieve these aims.
Connected Gene Communities Underlie Transcriptional Changes in Cornelia de Lange Syndrome

Cornelia de Lange syndrome (CdLS) is a complex multisystem developmental disorder caused by mutations in cohesin subunits and regulators. While its precise molecular mechanisms are not well defined, they point toward a global deregulation of the transcriptional gene expression program. Cohesin is associated with the boundaries of chromosome domains and with enhancer and promoter regions connecting the three-dimensional genome organization with transcriptional regulation. Here, we show that connected gene communities, structures emerging from the interactions of noncoding regulatory elements and genes in the three-dimensional chromosomal space, provide a molecular explanation for the pathoetiology of CdLS associated with mutations in the cohesin-loading factor NIPBL and the cohesin subunit SMC1A. NIPBL and cohesin are important constituents of connected gene communities that are centrally positioned at noncoding regulatory elements. Accordingly, genes deregulated in CdLS are positioned within reach of NIPBL- and cohesin-occupied regions through promoter–promoter interactions. Our findings suggest a dynamic model where NIPBL loads cohesin to connect genes in communities, offering an explanation for the gene expression deregulation in the CdLS.
Weight and body shape changes in a treatment-naive population after 6 months of nevirapine-based generic highly active antiretroviral therapy in South India.

BACKGROUND The nutritional and body shape response after the initiation of highly active antiretroviral therapy (HAART) in resource-limited environments has not been documented. In this environment, nutritional compromise is a common complication of human immunodeficiency virus (HIV) infection. METHODS We conducted a prospective study of 190 HIV-infected patients who initiated a nevirapine-based HAART regimen. CD4+ T cell count, body weight, body mass index, anthropometry, and bioelectrical impedance data were collected prior to initiation of therapy and after 6 months of therapy. RESULTS The mean age of participants was 35 years, 85% of participants were male, and 59% received stavudine as 1 of the nucleosides in their initial HAART regimen. The members of the cohort were malnourished before the initiation of therapy and had a mean body mass index of 20.1 (calculated as weight in kilograms divided by the square of height in meters). Overall, body weight increased a mean of 2.8 kg (range, -12.5 to 22.5 kg), and CD4+ T cell counts increased by a mean of 140 cells/mm3. Patients were stratified into those who lost weight (loss of >1 kg, 22%; n=41), those whose weight remained stable (19%; n=37), and those who gained weight (gain of >1 kg, 59%; n=112). Patients in all groups retained body shape symmetry and experienced no change in waist-to-hip ratio or regional body shape by anthropometry. CONCLUSIONS The group that lost weight and the group whose weight remained stable experienced significant CD4+ T cell count increases at 6 months. Although the majority of HIV-infected patients who received nevirapine-based HAART gained weight, there were participants who lost weight despite initiating their first HAART therapy.
Phosphorylation-Dependent Ubiquitination of Cyclin E by the SCFFbw7 Ubiquitin Ligase

Cyclin E binds and activates the cyclin-dependent kinase Cdk2 and catalyzes the transition from the G1 phase to the S phase of the cell cycle. The amount of cyclin E protein present in the cell is tightly controlled by ubiquitin-mediated proteolysis. Here we identify the ubiquitin ligase responsible for cyclin E ubiquitination as SCFFbw7 and demonstrate that it is functionally conserved in yeast, flies, and mammals. Fbw7 associates specifically with phosphorylated cyclin E, and SCFFbw7catalyzes cyclin E ubiquitination in vitro. Depletion of Fbw7 leads to accumulation and stabilization of cyclin E in vivo in human andDrosophila melanogaster cells. Multiple F-box proteins contribute to cyclin E stability in yeast, suggesting an overlap in SCF E3 ligase specificity that allows combinatorial control of cyclin E degradation.
Purification, sequencing and evaluation of a divergent phytase from Penicillium oxalicum KCTC6440.

A fungal strain producing high levels of phytase was purified to homogeneity from Penicillium oxalicum KCTC6440 (PhyA). The molecular mass of the purified PhyA was 65 kDa and optimal activity occurred at 55°C. The enzyme was stable in a pH range of 4.5-6.5, with an optimum performance at pH 5.5. The Km value for the substrate sodium phytate was 0.48 mM with a Vmax of 672 U/mg. The enzyme was inhibited by Ca(2+), Cu(2+), and Zn(2+), and slightly enhanced by EDTA. The PhyA efficiently released phosphate from feedstuffs such as soybean, rich bran and corn meal. The PhyA gene was cloned in two steps of degenerate PCR and inverse PCR and found to comprise 1501 bp and encode 461 amino acid residues. The enzyme was found to have only 13 amino acids differing to the known PhyA from other Penicillium sp. , but has distinct enzyme characteristics. Computational analysis showed that PhyA possessed more positively charged residues in the active sites compared to other PhyA molecules, which may explain the broader pH spectrum.
Best Practices in the Development, Translation, and Cultural Adaptation of Patient-Reported Outcome Measures for Adults With Hearing Impairment: Lessons From the Cochlear Implant Quality of Life Instruments

This manuscript summarizes available evidence-based best practices in the development, translation, and cultural adaptation of one type of outcome measure for adults with hearing impairment, patient-reported outcome measures (PROMs). It presents the development of the Cochlear Implant Quality of Life (CIQOL) instruments and the ongoing translation and cultural adaptation of the CIQOL-35 Profile from English to French as case studies and discusses useful lessons for selecting, developing, translating, culturally adapting, and using PROMs. Relevant best practice guides are introduced, described and their steps are illustrated with examples. Future trends in hearing-related PROMs, including computerized adaptive testing, patient-reported experience measures (PREMs), economic evaluation and allocation of scarce resources, and PROMs in low-resource settings, are discussed. The manuscript concludes on the lessons that can be learned from implementation science for the successful and sustainable integration of PROMs in clinical practice.