Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparative mycobactericidal efficacy of chemical disinfectants in suspension and carrier tests

The efficacy of nine disinfectants on Mycobacterium smegmatis was tested in the presence of sputum, using quantitative suspension and carrier tests. Glutaraldehyde, povidone iodine, and chlorhexidine gluconate produced at least a 6-log10 reduction in CFU in all tests. Four disinfectants (sodium dichloroisocyanurate, phenol, ethanol, and sodium hypochlorite) were not as effective in the carrier tests as in the suspension tests; this difference ranged from a 1- to a 5-log10 reduction in CFU. The efficacy of ethanol and sodium hypochlorite was further reduced (3- and 1-log10 reductions in CFU, respectively) in the presence of sputum. The quaternary ammonium compound and iodophor were ineffective in all tests. The findings of this study demonstrate the need for a quantitative carrier test such as the one presented here.
Analysis on data collection using mobile robot in wireless sensor networks

Recently, owing to the improvement of mass construction, the charge of sensor nodes has reduced considerably, and resource-rich sensor nodes can be prepared with high-technology procedures, for instance GPS. Nevertheless, the capability of the battery for all sensor nodes is measured inadequate in terms of energy. When energy diminution of sensor node fallout in a dead mode that is not capable to correspond with other nodes, this generates a bottleneck in a WSN. The data collection algorithm efficiently retrieved the sensed data from the dead node. Collecting sensed data using this approach will consume more time. Various techniques to approach the process of collecting sensed data using mobile robot in wireless sensor networks. In this paper, observed the latest sensed data collection methods and supply a summary in the applying automatic mobile robot with wireless sensor networks and also associated investigation work with that place. In addition side by side evaluations usually are done between various techniques to explain the advantages along with limitations. In this paper, experimental analysis has been conducted along with various factors to demonstrate the development as well as deployment of the sensed information collecting procedure inside wireless sensor networks.
Asymmetric Information in Simple Bargaining Games: An Experimental Study

Abstract Bilateral bargaining situations are often characterized by informational asymmetries concerning the size of what is at stake: in some cases, the proposer is better informed, in others, it is the responder. We analyze the effects of both types of asymmetric information on proposer behavior in two different situations which allow for a variation of responder veto power: the ultimatum and the dictator game. We find that the extent to which proposers demand less in the ultimatum as compared to the dictator game is (marginally) smaller when the proposer is in the superior information position. Further we find informed proposers to exploit their informational advantage by offering an amount that does not reveal the true size of the pie, with proposers in the ultimatum game exhibiting this behavioral pattern to a larger extent than those in the dictator game. Uninformed proposers risk imposed rejection when they ask for more than potentially is at stake, and ask for a risk premium in dictator games. We concentrate on proposers, but also explore responder behavior: We find uninformed responders to enable proposers’ hiding behavior, and we find proposer intentionality not to play an important role for informed responders when they decide whether to accept or reject an offer by an (uninformed) proposer.
An Alternating Direction Implicit Backward Differentiation Orthogonal Spline Collocation Method for Linear Variable Coefficient Parabolic Equations

We present a new fully discrete method to solve a linear variable coefficient parabolic equation on a rectangular region. The scheme is an alternating direction implicit method which uses the third order backward differentiation formula for the time discretization and piecewise Hermite bicubic orthogonal spline collocation for the spatial discretization. The $L^2$ norm stability and convergence analysis is carried out for the heat equation. The stability analysis reveals that the scheme is stable with respect to the right-hand sides but it is unstable with respect to the initial conditions. We explain, using a damping property of the scheme and round-off error analysis, why this instability is harmless. We also show how a different choice of initial values leads to optimal convergence orders. Numerical results demonstrate the third order accuracy in time and optimal order accuracy in space using maximum and Sobolev norms, respectively.
Notch activation induces neurite remodeling and functional modifications in SH‐SY5Y neuronal cells

Notch proteins are definitely recognized as key regulators of the neuronal fate during embryo development, but their function in the adult brain is still largely unknown. We have previously demonstrated that Notch pathway stimulation increases microtubules stability followed by the remodeling of neuronal morphology with neurite varicosities loss, thicker neuritis, and enlarged growth cones. Here we show that the neurite remodeling is a dynamic event, dependent on transcription and translation, and with functional implications. Exposure of differentiated human SH‐SY5Y neuroblastoma cells to the Notch ligand Jagged1 induces varicosities loss all along the neurites, accompanied by the redistribution of presynaptic vesicles and the decrease in neurotransmitters release. As evaluated by time lapse digital imaging, dynamic changes in neurite morphology were rapidly reversible and dependent on the activation of the Notch signaling pathway. In fact, it was prevented by the inhibition of the proteolytic γ‐secretase enzyme or the transcription machinery, and was mimicked by the transfection of the intracellular domain of Notch. One hour after treatment with Jagged1, several genes were downregulated. Many of these genes encode proteins that are known to be involved in protein synthesis. These data suggest that in adult neurons, Notch pathway activates a transcriptional program that regulates the equilibrium between varicosities formation and varicosities loss in the neuronal presynaptic compartment involving the expression and redistribution of both structural and functional proteins. © 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2009
An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates.

AIM The bactericidal effect of four antimicrobial agents was investigated against single-species biofilms derived from a range of root canal isolates. METHODOLOGY Single-species biofilms of Prevotella intermedia, Peptostreptococcus micros, Streptococcus intermedius, Fusobacterium nucleatum and Enterococcus faecalis were generated on membrane filter discs and subjected to 15 min or 1 h incubation with 5 p.p.m. colloidal silver, 2.25% sodium hypochlorite (NaOCl), 0.2% chlorhexidine, 10% iodine or phosphate buffered saline (PBS) as a control. The antimicrobial activity of the agents was neutralized and the bacterial cells were harvested from the discs by vortexing, serially diluted in reduced transport fluid, plated on fastidious anaerobe agar containing 5% horse blood, incubated anaerobically and colony-forming units calculated. RESULTS Iodine and NaOCl were more effective than chlorhexidine except against P. micros and P. intermedia where they were all 100% effective. Iodine and NaOCl elicited a 100% kill after 1 h incubation for all strains used. However, after 15 min, they showed differing bactericidal effects depending on the strain. None of the agents were effective against F. nucleatum after 15 min but NaOCl, iodine and chlorhexidine were all effective after 1 h. Colloidal silver was generally ineffective. CONCLUSIONS The effectiveness of a particular agent was dependent on the nature of the organism in the biofilm and on the contact time. NaOCl was generally the most effective agent tested, followed by iodine. However the clinical efficacy of these agents must be considered in light of the complex root canal anatomy and polymicrobial nature of root canal infections.
Comparative evaluation of on-line missing data regression techniques in intrapartum FHR measurements

Cardiotocography is a measurement technique used for fetal health status assessment in antepartum and intrapartum monitoring. A typical cardiotocogram recording consists of two simultaneously acquired signals, namely the fetal hearth rate (FHR) and the uterine activity (UA). Unfortunately, the FHR recordings suffer from frequent invalid or missing samples, due to artifacts or sensors misfunctions. In literature, this problem is typically solved by simplistic linear interpolations or sophisticated algorithms, whose computational complexity prevents from an on-line implementation. In this paper, we propose five regression techniques which rely exclusively on a reduced set of past samples, i.e. compliant with on-line implementation. We characterize their performances in terms of regression error, as well as specific clinical indices which account for the capability not to distort the original information content of the acquired signal.
Myofascial pain: an open study on the pharmacotherapeutic response to stepped treatment with tricyclic antidepressants and gabapentin.

AIMS To evaluate, in an open trial, the pharmacotherapeutic efficacy of tricyclic antidepressant (TCA) drugs and gabapentin in patients with persistent myofascial pain and to identify patient and pain characteristics that may predict treatment outcome. METHODS A stepped pharmacotherapeutic protocol was employed. All 42 patients having persistent facial pain with tenderness of regional muscles were first prescribed amitriptyline, but those with side effects were subsequently transferred to nortriptyline. In patients where no response to TCAs was observed, gabapentin was initiated. Outcome was assessed by employing prospective diaries recording pain intensity measured with an 11-point (0-10) verbal pain scale (VPS). Individual characteristics in these patients and their influence on drug response and outcome were analyzed; specifically, patients treated with TCAs were compared with those subsequently treated with gabapentin. Chi-square and t tests were used to analyze the data. RESULTS A total of 23 patients responded to TCAs and continued on this regimen, while 19 were resistant to TCAs and were subsequently treated with gabapentin. Their mean (± SD) VPS score at baseline was 6.5 ± 1.9 on an 11-point scale. In TCA-treated patients, 43% showed ≥ 50% reduction in pain intensity. This was achieved with a mean amitriptyline dose of 16 ± 1.1 mg/d and a mean nortriptyline dose of 25 ± 2.1 mg/d. Patients who did not respond to TCAs were characterized by a significantly higher age, more comorbid medical illness, and evidence of more regional pain spread (P < .05). In spite of not responding to TCAs, 36.8% of this group showed ≥ 50% reduction in pain intensity following gabapentin therapy at a mean daily dose of 973.7 ± 68.8 mg. Overall, a stepped approach employing TCAs and gabapentin resulted in 54.8% of all treated patients reporting improvements of ≥ 50% in VPS scores. CONCLUSION This study has demonstrated the good pharmacotherapeutic response of persistent myofascial pain, even in more severe cases. Not being a randomized controlled trial, the results may be biased and should be interpreted with caution. Patients who do not respond to TCAs may be a distinct subgroup and this needs further investigation. The results also suggest that gabapentin, at a lower dose than previously reported, is a good alternative in TCA-resistant patients.
GSTP1 CpG island hypermethylation as a molecular biomarker for prostate cancer

Somatic hypermethylation of CpG island sequences at GSTP1, the gene encoding the π‐class glutathione S‐transferase, appears to be characteristic of human prostatic carcinogenesis. To consider the potential utility of this epigenetic alteration as a biomarker for prostate cancer, we present here a comprehensive review of the literature describing somatic GSTP1 changes in DNA from prostate cells and tissues. GSTP1 CpG island hypermethylation has been detected in prostate cancer DNA using a variety of assay techniques, including (i) Southern blot analysis (SB), after treatment with 5‐mC‐sensitive restriction endonucleases, (ii) the polymerase chain reaction, following treatment with 5‐mC‐sensitive restriction endonucleases (RE‐PCR), (iii) bisulfite genomic sequencing (BGS), and (iv) bisulfite modification followed by the polymerase chain reaction, using primers selective for target sequences containing 5‐mC (MSP). In the majority of the case series so far reported, GSTP1 CpG island hypermethylation was present in DNA from at least 90% of prostate cancer cases. When analyses have been carefully conducted, GSTP1 CpG island hypermethylation has not been found in DNA from normal prostate tissues, or from benign prostatic hyperplasia (BPH) tissues, though GSTP1 CpG island hypermethylation changes have been detected in DNA from candidate prostate cancer precursor lesions proliferative inflammatory atrophy (PIA) and prostatic intraepithelial neoplasia (PIN). Using PCR methods, GSTP1 CpG island hypermethylation has also been detected in urine, ejaculate, and plasma from men with prostate cancer. GSTP1 CpG island hypermethylation, a somatic epigenetic alteration, appears poised to serve as a molecular biomarker useful for prostate cancer screening, detection, and diagnosis. © 2003 Wiley‐Liss, Inc.
Uni-direction Orienting and Size Effect Automatic Alignment Using Capillary Based in Fluidic Self-Assembly Process

Advances of miniaturization and microfluidics lead to novel technologies in assembling micro-scale electronic components in thousands and in parallel. Present study proposes a fluidic self-assembly (FSA) approach for fast, economic, and precise handling of micro-scale parts in asymmetric shapes. The micro parts, fabricated from silicon-oxide wafers and ranged in size from 350 x 350 x 170 mum to 1000 x 1000 x 440 mum , are aligned and filled to designated sites in the substrate under water using a pipette. The adhesion force of square-patterned micropart immobilized at the bigger binding sites was estimated at 117 +15 mu N, resulting in highter assembly yield of up to 73% for these sample. Furthermore, the novel designs of two dimensional tear-drop/rocking-chair like shapes are employed to improve the recovery angle and reduce the energy barrier for micropart alignment in uni-direction. Filling ratio of 90% is achieved with uni-direction if five hundred parts are filled to all of 64 binding sites with the agitation by an orbital shaker.
Neural Network–Based Classification of Anesthesia/Awareness Using Granger Causality Features

This article investigates the signal processing part of a future system for monitoring awareness during surgery. The system uses features from the patients’ electrical brain activity (EEG) to discriminate between “anesthesia” and “awareness. ” We investigate the use of a neural network classifier and Granger causality (GC) features for this purpose. GC captures anesthetic-induced changes in the causal relationships between pairs of signals from different brain areas. The differences in the pairwise causality estimated from the EEG activity are used as features for subsequent classification between “awake” and “anesthetized” states. EEG data from 31 subjects obtained during surgery and maintenance of anesthesia with propofol, sevoflurane, or desflurane, are classified using a neural network with one layer of hidden units. An average accuracy of 96% is obtained.
FRAND Arbitration: The Determination of Fair, Reasonable and Non-Discriminatory Rates for SEPs by Arbitral Tribunals

At the core of most disputes concerning the licensing of standard-essential patents (SEPs) lies the inability of the SEP holder and the standard implementer to agree on fair, reasonable and non-discriminatory (FRAND) license terms. As an alternative to court litigation, a growing number of academics, agency officials and private practitioners have advocated arbitration of SEP-related disputes, and there is anecdotal evidence that are increasingly relying on arbitration to settle such disputes. The purpose of this paper is to discuss based on the author’s personal experience how arbitral proceedings to set FRAND terms work in practice, as well as the various challenges faced by arbitrators, parties, and counsel involved in such proceedings.
Mitochondrial phylogeography of the Woodmouse (Apodemus sylvaticus) in the Western Palearctic region

We sequenced 965 base pairs of the mitochondrial DNA cytochrome b from 102 woodmice (Apodemus sylvaticus) collected from 40 European localities. The aims of the study were to answer the following questions. (i) Did the Mediterranean peninsulas play a role as refuge for woodmice? (ii) Is genetic variability of A. sylvaticus higher in the Mediterranean region compared with northern Europe? (iii) Are the patterns of the postglacial colonization of Europe by woodmice similar to those presently recognized for other European species? The results provide a clear picture of the impact of the Quaternary glaciations on the genetic and geographical structure of the woodmouse. Our analyses indicate a higher genetic variability of woodmice in the Mediterranean peninsulas compared to northern Europe, suggesting a role of the former as refuge regions for this small mammal. An original pattern of postglacial colonization is proposed where the Iberian and southern France refuge populations colonized almost all European regions. The Sicilian population appears to be very differentiated and highly variable. This emphasizes the importance of this island as a ‘hot spot’ for the intraspecific genetic diversity of the woodmouse. Finally, woodmice in North Africa originated from southwestern Europe, most probably as a result of a recent anthropogenic introduction.
Longitudinal monitoring of power output and heart rate profiles in elite cyclists

Abstract Power output and heart rate were monitored for 11 months in one female ([Vdot]O2max: 71.5 mL · kg−1 · min−1) and ten male ([Vdot]O2max: 66.5 ± 7.1 mL · kg−1 · min−1) cyclists using SRM power-meters to quantify power output and heart rate distributions in an attempt to assess exercise intensity and to relate training variables to performance. In total, 1802 data sets were divided into workout categories according to training goals, and power output and heart rate intensity zones were calculated. The ratio of mean power output to respiratory compensation point power output was calculated as an intensity factor for each training session and for each interval during the training sessions. Variability of power output was calculated as a coefficient of variation. There was no difference in the distribution of power output and heart rate for the total season (P = 0.15). Significant differences were observed during high-intensity workouts (P < 0.001). Performance improvements across the season were related to low-cadence strength workouts (P < 0.05). The intensity factor for intervals was related to performance (P < 0.01). The variability in power output was inversely associated with performance (P < 0.01). Better performance by cyclists was characterized by lower variability in power output and higher exercise intensities during intervals.
The influence of experience and deliberate learning on SME export performance

Purpose The purpose of this paper is to determine the relative importance of deliberate learning, learning from experience and relevant learning co-variates in pursuing market learning, and to assess the impact of market learning on export performance in smaller firms. Design/methodology/approach A theoretical model was initially developed and subsequently tested using survey data. The standard two step approach of first testing the measurement model and then estimating the structural model was adopted. Findings The results provide concrete evidence that among SMEs, deliberate learning has a greater impact on export market learning as compared to experience accumulation, and that market learning has a significant effect on export performance. The results also show that absorptive capacity and commitment to learning are significant co-variates of market learning. Originality/value This paper focuses on the role of deliberate learning vis-a-vis learning by experience in achieving foreign market learning and export performance in smaller firms. It addresses a major limitation of organisational learning studies which tend to focus mainly on experiential learning and organisational learning in large organisations.
Cutting Edge: Vasoactive Intestinal Peptide (VIP) Induces Differentiation of Th17 Cells with a Distinctive Cytokine Profile1

Immune cellular effects of vasoactive intestinal peptide (VIP) are transduced by VIP G protein-coupled receptors type 1 (VPAC1) and type 2 (VPAC2). We now show that VIP with TGFβ stimulates the transformation of CD4 T cells to a distinctive type of Th17 cell that generates IL-17 but not IL-6 or IL-21. VIP induction of Th17 cells was higher in VPAC2 knockout mice than wild-type mice, suggesting that VPAC1 is the principal transducer. Compared with Th17 cells elicited by IL-6, those evoked by VIP were similar in the secretion of IL-17 and IL-22, but lacked IL-21 secretion. Suppression of VIP induction of Th17 cells by protein kinase A inhibitors and enhancement by pharmacologically increased cAMP supports a role for this signal. The ability of VIP-VPAC1 axis signals to evoke development of a novel type of Th17 cells demonstrates the unique specificity of neuroregulatory mechanisms in the immunological environment.
Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis

Background Serologic diagnosis is one of the most widely used diagnostic methods for Q fever, but the window period in antibody response of 2 to 3 weeks after symptom onset results in significant diagnostic delay. We investigated the diagnostic utility of Q fever PCR from formalin-fixed liver tissues in Q fever patients with acute hepatitis. Methods We reviewed the clinical and laboratory data in patients with Q fever hepatitis who underwent liver biopsy during a 17-year period, and whose biopsied tissues were available. We also selected patients who revealed granuloma in liver biopsy and with no Q fever diagnosis within the last 3 years as control. Acute Q fever hepatitis was diagnosed if two or more of the following clinical, serologic, or histopathologic criteria were met: (1) an infectious hepatitis-like clinical feature such as fever (≥ 38°C) with elevated hepatic transaminase levels; (2) exhibition of a phase II immunoglobulin G (IgG) antibodies titer by IFA of ≥ 1:128 in single determination, or a four-fold or greater rise between two separate samples obtained two or more weeks apart; (3) histologic finding of biopsy tissue showing characteristic fibrin ring granuloma. Results A total of 11 patients with acute Q fever hepatitis were selected and analyzed. Of the 11 patients, 3 (27%) had exposure to zoonotic risk factors and 7 (63%) met the serologic criteria. Granulomas with either circumferential or radiating fibrin deposition were observed in 10 cases on liver biopsy and in 1 case on bone marrow biopsy. 8 (73%) revealed positive Coxiella burnetii PCR from their formalin-fixed liver tissues. In contrast, none of 10 patients with alternative diagnosis who had hepatic granuloma revealed positive C. burnetii PCR from their formalin-fixed liver tissues. Conclusions Q fever PCR from formalin-fixed liver tissues appears to be a useful adjunct for diagnosing Q fever hepatitis.