Miracle-dz/UniFAR-FTUs huggingface dataset:
Efficient expression of stable recombinant human insulin-like growth factor-1 fusion with human serum albumin in Chinese hamster ovary cells

ABSTRACT Insulin-like growth factor-1 (IGF-1) plays a crucial role in cell development, differentiation, and metabolism, and has been a potential therapeutic agent for many diseases. Chinese hamster ovary (CHO) cells are widely used for production of recombinant therapeutic proteins, but the expression level of IGF-1 in CHO cells is very low (1,500 µg/L) and the half-life of IGF-1 in blood circulation is only 4.5 min according to previous studies. Therefore, IGF-1 was fused to long-circulating serum protein human serum albumin (HSA) and expressed in CHO cells. After 8-day fed-batch culture, the expression level of HSA–IGF-1 reached 100 mg/L. The fusion protein HSA–IGF-1 was purified with a recovery of 35% using a two-step chromatographic procedure. According to bioactivity assay, the purified HSA–IGF-1 could stimulate the proliferation of NIH3T3 cells in a dose-dependent fashion and promote the cell-cycle progression. Besides this, HSA–IGF-1 could bind to IGF-1 receptor on cell membrane and activate the intracellular PI3K/AKT signaling pathway. Our study suggested that HSA fusion technology carried out in CHO cells not only provided bioactivity in HSA–IGF-1 for further research but also offered a beneficial strategy to produce other similar cytokines in CHO cells.
New approach with Secant method for solving Unit Commitment problem

This paper presents an application of Secant method and Improved pre-prepared power demand (IPPD) table for solving unit commitment (UC) problem. The UC problem is divided into combinatorial sub problem in unit status variables and non-linear programming sub problem in unit output power variables. Initially, IPPD table obtains the unit status information and then the optimal solution is achieved by Secant method at each power demand for 24 hours. The logic is used to obtain the IPPD table is at any power demand, the generating units, which are having low minimum incremental cost (lambda) are operating first and then the remaining generating units are kept in off position until meet the required power demand The proposed method has been tested on a power system having 3 and 10 generating units. The extensive studies have been performed for large-scale power system by considering 20, 40, 60, 80 and 100 generating units. The simulation results of the proposed method were compared with conventional methods such as dynamic programming and lagrangian relaxation method, heuristic methods such as genetic algorithm and simulated annealing and hybrid methods such as LR-GA in terms of solution quality and computational time. The comparison of results of the proposed method with the results of previous published methods shows that the proposed approach provides more qualitative solution with less computational time.
α11β1 integrin‐mediated MMP‐13‐dependent collagen lattice contraction by fibroblasts: Evidence for integrin‐coordinated collagen proteolysis

We have previously determined that integrin α11β1 is required on mouse periodontal ligament (PDL) fibroblasts to generate the force needed for incisor eruption. As part of the phenotype of α11−/− mice, the incisor PDL (iPDL) is thickened, due to disturbed matrix remodeling. To determine the molecular mechanism behind the disturbed matrix dynamics in the PDL we crossed α11−/− mice with the Immortomouse and isolated immortalized iPDL cells. Microarray analysis of iPDL cells cultured inside a 3D collagen gel demonstrated downregulated expression of a number of genes in α11‐deficient iPDL cells, including matrix metalloproteinase‐13 (MMP‐13) and cathepsin K. α11−/− iPDL cells in vitro displayed disturbed interactions with collagen I during contraction of attached and floating collagen lattices and furthermore displayed reduced MMP‐13 protein expression levels. The MMP‐13 specific inhibitor WAY 170523 and the Cathepsin K Inhibitor II both blocked part of the α11 integrin‐mediated collagen remodeling. In summary, our data demonstrate that in iPDL fibroblasts the mechanical strain generated by α11β1 integrin regulates molecules involved in collagen matrix dynamics. The positive regulation of α11β1‐dependent matrix remodeling, involving MMP‐13 and cathepsin K, might also occur in other types of fibroblasts and be an important regulatory mechanism for coordinated extracellular and intracellular collagen turnover in tissue homeostasis. J. Cell. Physiol. © 2012 Wiley Periodicals, Inc.
Implications of Habit Formation for Optimal Monetary Policy

We study the implications for optimal monetary policy of introducing habit formation in consumption into a general equilibrium model with sticky prices. Habit formation affects the model's endogenous dynamics through its effects on both aggregate demand and households' supply of output. We show that the objective of monetary policy consistent with welfare maximization includes output stabilization, as well as inflation and output gap stabilization. We find that the variance of output increases under optimal policy, even though it acquires a higher implicit weight in the welfare function. We also find that a simple interest rate rule nearly achieves the welfare-optimal allocation, regardless of the degree of habit formation. In this rule, the optimal responses to inflation and the lagged interest rate are both declining in the size of the habit, although super-inertial policies remain optimal.
Residents and Transients: Community Reaction to Seasonal Visitors

The residents of seasonal resorts must annually accommodate to the influx of large numbers of vacationers. This study conducted in two beach communities reveals that vacationers impact many aspects of people's lives, causing them to cur tail certain activities and causing deterioration of some parts of their communities. However, residents are also economically dependent upon visitors and benefit from the services and facilities which cater to tourists. On balance, most permanent resi dents are satisfied with their communities and their development. Hence, it is appar ent that communities can reconcile the demands of residential and commercial growth without destructive conflict.
The pathophysiology of the collateral circulation (arteriogenesis)

Since the mid 1980s a new strategy is coming from bench to bedside termed angiogenesis. This process involves sprouting of capillaries and finally results in newly developed microvessels which belong to the capillary level. Importantly these newly formed capillary tubes lack vascular smooth muscle cells, they are not surrounded by mural cells and are fragile and prone to rupture. Therefore these networks remain susceptible to hypoxic regulation, fail to become remodelled and are unable to sustain proper circulation: they cannot adapt to changes in physiological demands of blood supply. Since atherosclerosis affects large conductance arteries, capillary sprouting from compromised vessels cannot provide an adequate supply of blood flow to the endangered tissue. However, the body provides a natural system of pre‐existing collateral arteries, which may bypass sites of arterial occlusion. These vessels can dramatically increase their lumen by growth so as to provide enhanced perfusion to the jeopardized ischaemic regions. This process – termed arteriogenesis – finally results in fully functional and structurally normal arteries which can ameliorate the ensuing detrimental effects of vessel obstruction in many regions of the body. Hallmarks of arteriogenesis are increased levels of shear forces (rather than ischaemia), the invasion of circulating monocytes (and their pluripotent precursors), and the substrates of arteriogenesis are pre‐existing colateral arterioles. Copyright © 2000 John Wiley & Sons, Ltd.
The Impact of U.S. Regional Business Cycles on Remittances to Latin America

The current economic slowdown in the United States and the decline in remittance growth to some Latin American countries have intensified the interest in the relationship between these variables. We investigate whether host country conditions affect remittance outflows to Latin America, focusing on the roles of regional U.S. business cycles, geographical variation in immigrant density and sectoral factors. Using quarterly data for 1995-2008, we find that remittance flows are strongly influenced by economic conditions in the specific regions of the U.S. where migrants are clustered, as well as in the sectors especially important for immigrants' employment opportunities. The results are in sharp contrast to previous research suggesting that remittance flows are relatively insensitive to fluctuations in the aggregate U.S. business cycle. Precise estimation of these linkages is also shown to matter for gauging the sensitivity of remittances to economic conditions in the home country, and hence the extent to which remittances might buffer domestic shocks as well as transmitting external ones.
A sensitivity study on modeling black carbon in snow and its radiative forcing over the Arctic and Northern China

Black carbon in snow (BCS) simulated in the Community Atmosphere Model (CAM5) is evaluated against measurements over Northern China and the Arctic, and its sensitivity to atmospheric deposition and two parameters that affect post-depositional enrichment is explored. Improvements in atmospheric BC transport and deposition significantly reduce the biases (by a factor of two) in the estimation of BCS concentration over both Northern China and the Arctic. Further sensitivity simulations using the improved CAM5 indicate that the melt-water scavenging efficiency (MSE) parameter plays an important role in regulating BC concentrations in the Arctic through the post-depositional enrichment, which not only drastically changes the amplitude but also shifts the seasonal cycle of the BCS concentration and its radiative forcing in the Arctic. The impact of the snow aging scaling factor (SAF) on BCS shows more complex latitudinal and seasonal dependence, and overall impact of SAF is much smaller than that of MSE. The improvements of BC transport and deposition in CAM5 have a stronger influence on BCS than perturbations of the two snow model parameters in Northern China.
Quality standards development in a hypercompetitive scenario

Standards play an increasingly important role in the development of the “global village”, both from the economic and the quality of life perspective. However, development of standards should always be in tune with the needs of a free economy, whose main engine is differentiation. Standardization and differentiation can be complementary, but only to the extent that a correct balance between the two is kept. The balance becomes particularly delicate when standards move from product‐related issues to organization‐related issues, as is the case of quality system standards. Starting from a brief review of ISO 9000 and its role in the past decade, then moving to Vision 2000, the paper expresses reservations about the benefits and opportunity for standards to enter the organizational improvement area. In a situation of continuous change and global competition, differentiation seems to be the name of the game, more than standardization. Instead, a kind of “standards syndrome” seems to take place, where standards are expected to legitimize managers’ choices.
Hedging long‐term commodity risk: A comment

Y. V. Veld‐Merkoulova and F. A. de Roon (2003) adopted an encompassing model to demonstrate their linear yield assumption on the term structure of futures prices gains more empirical support than the linear price assumption proposed by A. Neuberger (1999). This comment points out the test procedure adopted is inappropriate and proposes an alternative non‐nested hypothesis testing method. Using the crude oil data, we find that the linear price assumption outperforms the linear yield assumption but is inferior to a generalized version of the linear yield assumption. © 2004 Wiley Periodicals, Inc. Jrl Fut Mark 24:1093–1099, 2004
Financial reporting complexity and investor underreaction to 10-K information

We study the immediate and delayed market reaction to U.S. Securities and Exchange Commission (SEC) EDGAR 10-K filings. Unusual trading volumes and stock-price movements are documented during the days around the 10-K filing dates. The abnormal price movements are positively associated with future accounting profitability, indicating that 10-K reports contain useful information about future firm performance. In addition, investors’ reaction to 10-K information seems sluggish, as demonstrated by the stock-price drift during the 12-month period after 10-K filing. We find that investors’ underreaction tends to be stronger for firms with more complex 10-K reports.
Normative Behavioural Economics Based on Unconditional Love and Moral Virtue

An important difficulty in many models of behavioural economics is that preferences are endogenous and unstable. Therefore, preferences may not provide the most desirable yardstick to evaluate social states. The present paper proposes unconditional love as a candidate for such a yardstick. The concept of unconditional love, although lofty, is often hard to apply for practical policy recommendations. We propose an intermediary learning stage, where learning to unconditionally love is desirable, and policies that promote such learning are deemed to be good. We illustrate the use of this principle in models of endogenous altruism.
An Ant colony optimization for Weighted Traveling Salesman Problem and analysis

The traveling salesman problem (TSP) is a well-known combinatorial optimization problem. While, in classical TSP and its variants, the priority of each city is assumed as the same. The objective function neglects extra vehicle loading cost caused by practical factors when optimizing a route. However, in real-world, the priority varies from one city to another and affects the loading cost. Thus, a route without considering the effect of different priority of each city may be not reasonable enough and may lead to sub-optimal solutions. In this paper, we investigate Weighted Traveling Salesman Problem (WTSP), the cost of which concluded both distance cost and the extra loading cost when finishing a route. Considering the features of WTSP model, a Mutated Max-Min Ant System is proposed. Computational results of benchmark instances with five types of distributions are shown to prove that the M-MMAS is competitive and superior to other algorithms for most instances and that WTSP formulation is reasonable and can save more money for practical factors.
Determination of the prospective energy consumption of manufacturing technologies with methods-energy measurement (MEM)

The reduction of energy costs of manufacturing technologies is both an important and current objective for factory operators. But mostly, this idea to reduce the energy consumption is being implemented after the manufacturing technologies are installed. However, it would be more advantageous if efforts were made to save energy during the early planning process. To achieve an estimation of the energy consumption in this early planning stage, a new suitable forecast method is required. Therefore, this paper shows how the prospective energy consumption can be determined by using Methods-Energy Measurement (MEM). Based on the needs of production planners, future challenges and requirements for MEM are listed. Further-more, the concept of basic energy elements and their value determination techniques are introduced, and different possibilities are outlined and assessed. For an easier determination of the energy demands of a complex production cell or manufacturing equipment, standard equipment patterns will be designed. This template supports both planners and decision-makers to create reliable and standardized Resource Performance Indicators (RPI). Subsequently, the architecture of the MEM-calculation model is presented. This approach enables a holistic estimation of the energy consumption of manufacturing technologies and leads to higher levels of transparency. Furthermore, MEM contributes to identify and realize sustainable solutions in advance and as a result, increases the profitability of a factory.
Systematic Characterization of Tunnel FETs Using a Universal Compact Model

We propose a novel systematic procedure to individually characterize relevant physical phenomena that typify tunnel FETs (TFETs) in general. The procedure uses the Lambert $W$ function to explicitly solve the subthreshold and above-threshold transfer characteristics of TFETs, as described by a recently proposed universal TFET compact model based on Kane's tunneling formulation. The resulting explicit analytic solutions may be used to numerically calculate, and thus to reveal, the terminal voltage-dependent functionalities of the device's subthreshold current swing and effective electric field. The description of the output characteristics' gate-to-source voltage dependence and saturation is also part of the procedure. The resulting functional representations of the relevant features constitute useful tools for basic device analysis and design, as well as for formulating specific mathematical descriptive models of these features. Atomistic, quantum-mechanical simulated data of an InAs homojunction TFET are used as an example to numerically and graphically illustrate the proposed characterization procedure.
Design of a Joint Defense System for Mobile Ad Hoc Networks

A mobile ad hoc network (MANET) is vulnerable to malicious attacks although it is suitable for various environments because of its rapid establishment. In order to set up a secured MANET, we should not only adopt encryption and authentication, but also equip each node with an intrusion detection system to detect malicious attackers. Focusing on intrusion detection, we propose an intrusion detection system that integrates a finite state machine (FSM) and a support vector machine (SVM) to analyze traffic patterns of MANETs. Shown by numerical examples, such an intrusion detection system is able to amend drawbacks of single-technique systems and enhance usage/right of normal users as well as security of MANETs
Cloud Program with a Pricing Strategy for Iaas in Cloud Computing

Cloud computing is the latest technology trend in delivering computing resources as a service. In practice, cloud service providers tend to offer services that can be grouped into three categories: software as a service (Saas), platform as a service (Paas) and infrastructure as a service (Iaas). Iaas is the delivery of hardware (server, storage and network) as a service. Cloud computing moves computations and data away from desktop PCs into large data centers. Traditional programs cannot bridge the separation between the possession and ownership of program from its use. To address this issue, cloud program with a pricing strategy for Iaas is proposed for cloud computing. Experimental results demonstrate that the proposed program allows on-demand uses of cloud resources and provides pay-as-you-go services, which outperforms traditional programs in cloud computing.
MIMO Antenna Using Hybrid Electric and Magnetic Coupling for Isolation Enhancement

This paper presents a multiple-input and multiple-output antenna using a wideband decoupling structure for enhancing isolation. The decoupling structure is formed by a split ring, which consists of an inductive line and a capacitive gap to realize magnetic and electric coupling, respectively. By introducing the electric coupling in addition to the magnetic coupling, more degrees of freedom are obtained and the original coupling between antennas can be canceled out within a wide frequency band. Meanwhile, the performance of each antenna element is maintained, such as impedance bandwidth and radiation characteristics. Theoretical analysis and experimental verification are carried out. When the decoupling structure is placed between these two monopole antennas with edge-to-edge space of only 5 mm (<inline-formula> <tex-math notation="LaTeX">$0.04\lambda _{0}$ </tex-math></inline-formula> at 2.5 GHz), more than 20 dB isolation is realized over the bandwidth of 2.3–2.9 GHz (24%) with <inline-formula> <tex-math notation="LaTeX">$S_{11}$ </tex-math></inline-formula> and <inline-formula> <tex-math notation="LaTeX">$S_{22}$ </tex-math></inline-formula> less than −10 dB. Moreover, the isolation levels reach more than 30 dB over the frequency band of 2.3–2.8 GHz. Compared with the previous related works using decoupling networks and neutralization line techniques, the proposed decoupling structure can provide higher isolation of over 30 dB in a wider bandwidth.
One size does not fit all: Managing IT employees' employment arrangements

As alternative employment arrangements proliferate within the information technology (IT) function, it becomes increasingly important to understand the impact of these arrangements on IT employees. A prevalent notion in the IT literature is that these employees are homogeneous in their work values and that they prefer similar employment arrangements. Given the inefficiency of designing individual employment programs, we advocate a middle ground between the two extremes of individualized employment arrangements and “one size fits all. ” We conducted two studies. The first study developed an individual's work values profile as a psychological construct. It used a national sample of IT employees to validate a simple, heuristic procedure that was successful in classifying about 80% of the sample into three work values profiles.The second study demonstrated the use of work values profiles for understanding how employment arrangements differentially influence employee satisfaction. It applied the validated procedure to a single organization in order to demonstrate the general applicability of the procedure and to show that it provides researchers and HR professionals with better insights than the assumption that all IT employees are alike. © 2007 Wiley Periodicals, Inc.
The Incentive Effects of No Fault Automobile Insurance

Abstract This paper presents a theoretical and empirical analysis of the effects of no‐fault automobile insurance on fatal accident rates. As a mechanism for compensating the victims of automobile accidents, no‐fault insurance has several important advantages over the tort system. However, by restricting access to tort, no‐fault may weaken incentives for careful driving and lead to higher accident rates. We conduct an empirical analysis of automobile accident fatality rates in all U.S. states over the period 1968–94, controlling for the potential endogeneity of no‐fault laws. The results support the hypothesis that no‐fault is significantly associated with higher fatal accident rates than tort.
Seven Bacteriophages Isolated from the Female Urinary Microbiota

ABSTRACT Recent research has debunked the myth that urine is sterile, having uncovered bacteria within the bladders of healthy individuals. However, the identity, diversity, and putative roles of bacteriophages in the bladder are unknown. We report the draft genome sequences of seven bacteriophages isolated from microbial communities from adult female bladders.