Miracle-dz/UniFAR-FTUs huggingface dataset:
Maternal occupational exposure to organic solvents during early pregnancy and risks of neural tube defects and orofacial clefts

Objectives Though toxicological experiments demonstrate the teratogenicity of organic solvents in animal models, epidemiologic studies have reported inconsistent results. Using data from the population-based National Birth Defects Prevention Study, the authors examined the relation between maternal occupational exposure to aromatic solvents, chlorinated solvents and Stoddard solvent during early pregnancy and neural tube defects (NTDs) and orofacial clefts (OFCs). Methods Cases of NTDs (anencephaly, spina bifida and encephalocoele) and OFCs (cleft lip ± cleft palate and cleft palate alone) delivered between 1997 and 2002 were identified by birth defect surveillance registries in eight states; non-malformed control infants were selected using birth certificates or hospital records. Maternal solvent exposure was estimated by industrial hygienist review of self-reported occupational histories in combination with a literature-derived exposure database. ORs and 95% CIs for the association between solvent class and each birth defect group and component phenotype were estimated using multivariable logistic regression, adjusting for maternal age, race/ethnicity, education, pre-pregnancy body mass index, folic acid supplement use and smoking. Results The prevalence of exposure to any solvent among mothers of NTD cases (n=511), OFC cases (n=1163) and controls (n=2977) was 13.1%, 9.6% and 8.2%, respectively. Exposure to chlorinated solvents was associated with increased odds of NTDs (OR=1.96, CI 1.34 to 2.87), especially spina bifida (OR=2.26, CI 1.44 to 3.53). No solvent class was strongly associated with OFCs in these data. Conclusions The findings suggest that maternal occupational exposure to chlorinated solvents during early pregnancy is positively associated with the prevalence of NTDs in offspring.
Management strategies for osteochondritis dissecans of the knee in the skeletally immature athlete.

SYNOPSIS Osteochondritis dissecans (OCD) is a disorder resulting in focal breakdown of the subchondral bone, with potential disruption of the overlying articular cartilage. The femoral condyles of the knee are the most common locations for OCD, and the incidence of OCD appears to be increasing among active children. Juvenile OCD (JOCD) can be distinguished from adult OCD by the presence of open growth plates. Due to a lack of evidence on its early diagnosis, optimal treatment, and long-term course of healing, JOCD presents a unique challenge for the health care team. Approximately 50% to 67% of stable JOCD lesions heal successfully with nonoperative treatment. For unstable lesions and stable lesions that fail nonoperative treatment, a variety of surgical interventions can be utilized to stimulate bony healing and address articular cartilage lesions. It is recommended that rehabilitation of JOCD be tailored to the individual patient, based on the stage and radiographic status of the lesion and the mode of surgery employed when surgically addressed. Although there is a growing body of literature on this condition, the etiology and optimal methods for treatment, rehabilitation, and evaluating outcomes remain inconclusive due to a lack of quality evidence. LEVEL OF EVIDENCE Therapy, level 5.
Chronic HBV Infection outside Treatment Guidelines: Is Treatment Needed?

Background There are now seven antivirals approved for use in the management of chronic HBV infection in the US. Current professional guidelines recommend the use of antiviral treatment in only a distinct subset of the total HBV chronically infected population, estimated to be more than 350 million worldwide. The subset of chronically HBV-infected individuals for whom the antivirals have been demonstrated to produce desirable outcomes are those with abnormal liver enzymes and a viral load above a defined threshold, presumably identifying those at highest risk for development of cirrhosis and hepatocellular carcinoma. However, some individuals whose clinical features place them outside these guidelines, for whom treatment is not recommended, are also at significant risk for liver disease complications and liver-related death. Methods In this report, we produce new estimates of the age-specific risks of liver-related death in people outside the current treatment guidelines using published data from multiple populations. Results Our results indicate that the age-specific 10-year risks of liver-related mortality in these individuals range from 0.3–4% in the West to 0.3–20% in Asia. Conclusions The magnitude of these risks and the estimated size of the global population that falls outside of current treatment guidelines have led us to consider whether medical interventions are also needed for these individuals, either with currently approved therapeutics or yet-to-be-discovered medications targeting new mechanisms of antiviral effect. Potential targets for development of new medications are discussed.
Distinct roles of autophagy-dependent and -independent functions of FIP200 revealed by generation and analysis of a mutant knock-in mouse model

Chen et al. generated a FIP200-4A mutant knock-in mouse model and found that specifically blocking FIP200 interaction with Atg13 abolishes autophagy in vivo. Analysis of the new mouse model showed that nonautophagic functions of FIP200 are sufficient to fully support embryogenesis by maintaining a protective role in TNFα-induced apoptosis. However, FIP200-mediated canonical autophagy is required to support neonatal survival and tumor cell growth.
Oligomerization through Hemopexin and Cytoplasmic Domains Regulates the Activity and Turnover of Membrane-type 1 Matrix Metalloproteinase*

The formation of multimeric complexes by membrane-type 1 matrix metalloproteinase (MT1-MMP) may facilitate its autocatalytic inactivation or proMMP-2 activation on the cell surface. To characterize these processes, we expressed various glutathione S-transferase/MT1-MMP fusion proteins in human HT-1080 fibrosarcoma cells and SV40-transformed lung fibroblasts and analyzed their effects on MT1-MMP activity and potential homophilic interactions. We report here that MT1-MMP is expressed on the cell surface as oligomeric 200–240-kDa complexes containing both the active 60-kDa and autocatalytically processed 43-kDa species. Overexpression of a glutathione S-transferase/MT1-MMP fusion protein containing the transmembrane and cytoplasmic domains of MT1-MMP inhibited the phorbol 12-myristate 13-acetate-induced autocatalytic cleavage of endogenous MT1-MMP to the 43-kDa species, but not proMMP-2 activation. On the other hand, a similar fusion protein with the hemopexin, transmembrane, and cytoplasmic domains inhibited proMMP-2 activation in a dominant-negative fashion. These results suggest that both the autocatalytic cleavage of MT1-MMP and proMMP-2 activation may be regulated by oligomerization through the cytoplasmic and hemopexin domains. Indeed, either domain, when attached to the cell membrane by a transmembrane domain, formed stable homophilic complexes. Copurification of MT1-MMP with these fusion proteins correlated with their cell-surface co-localization. Thus, MT1-MMP oligomerization through the hemopexin, transmembrane, and cytoplasmic domains controls its catalytic activity.
Traumatic brain injury in high school athletes.

CONTEXT The potential seriousness of mild traumatic brain injury (MTBI) is increasingly recognized; however, information on the frequency of MTBI among high school athletes is limited. OBJECTIVE To identify the type, frequency, and severity of MTBI in selected high school sports activities. DESIGN Observational cohort study. SETTING AND PARTICIPANTS Two hundred forty-six certified athletic trainers recorded injury and exposure data for high school varsity athletes participating in boys' football, wrestling, baseball and field hockey, girls' volleyball and softball, boys' and girls' basketball, and boys' and girls' soccer at 235 US high schools during 1 or more of the 1995-1997 academic years. MAIN OUTCOME MEASURES Rates of reported MTBI, defined as a head-injured player who was removed from participation and evaluated by an athletic trainer or physician prior to returning to participation. National incidence figures for MTBI also were estimated. RESULTS Of 23566 reported injuries in the 10 sports during the 3-year study period, 1219 (5.5%) were MTBIs. Of the MTBIs, football accounted for 773 (63.4%) of cases; wrestling, 128 (10.5%); girls' soccer, 76 (6.2%); boys' soccer, 69 (5.7%); girls' basketball, 63 (5.2%); boys' basketball, 51 (4.2%); softball, 25 (2.1%); baseball, 15 (1.2%); field hockey, 13 (1.1%); and volleyball, 6 (0.5%). The injury rates per 100 player-seasons were 3.66 for football, 1.58 for wrestling, 1.14 for girls' soccer, 1.04 for girls' basketball, 0.92 for boys' soccer, 0.75 for boys' basketball, 0.46 for softball, 0.46 for field hockey, 0.23 for baseball, and 0.14 for volleyball. The median time lost from participation for all MTBIs was 3 days. There were 6 cases of subdural hematoma and intracranial injury reported in football. Based on these data, an estimated 62816 cases of MTBI occur annually among high school varsity athletes participating in these sports, with football accounting for about 63% of cases. CONCLUSIONS Rates of MTBI vary among sports and none of the 10 popular high school sports we studied is without the occurrence of an MTBI. Continued involvement of high school sports sponsors, researchers, medical professionals, coaches, and sports participants is essential to help minimize the risk of MTBI.
Implementation of DWM for medical images using IWT and QR code as a watermark

As we know that, securing the medical images is a very challenging task. This challenge is efficiently handled with the help digital watermarking techniques. With the help of this growing technology we are able to evaluate validation, reliability, privacy and integrity of the medical images. Many algorithms have been implemented on this technology. The DWM may be implemented using various techniques like DCT, DWT, combination of DCT and DWT, combination of DWT and SVD. Nowadays the work is extended using integer wavelet transform (IWT) also [5]. But in all these works, one of the major challenges is data embedding capacity. The more number of bits hidden distorts the image which is strictly avoided in the case of medical and military applications. To overcome this problem nowadays work is going on to implement digital watermarking technique with less data embedding capacity which may be achieved by converting the watermark in to quick response code (QR Code). The QR code consumes a less space for large information compared to any other existing formats. In this paper an approach is proposed to implement the digital watermarking technique for the medical images which comprises of the techniques: Integer wavelets transform (IWT), Bit plane method and quick response code (QR code). After the watermarking process, the watermarked image is evaluated against some of the parameters to know the efficiency of technique being employed. The experiment is carried out for two different bit planes and the results are compared to show embedding in which of the bit plane number results in more efficiency and finally the conclusion is made.
Combination Therapy of Intense Pulsed Light Therapy and Meibomian Gland Expression (IPL/MGX) Can Improve Dry Eye Symptoms and Meibomian Gland Function in Patients With Refractory Dry Eye: A Retrospective Analysis

Purpose: To assess the improvement in meibomian gland function and dry eye symptoms in patients with refractory dry eye treated with a combination therapy of intense pulsed light (IPL) and meibomian gland expression (MGX). Methods: Medical records of 81 consecutive patients with dry eye treated with serial IPL/MGX were retrospectively examined to determine the outcome. All patients had a minimum of 6 months of follow-up after the first IPL/MGX treatment. Patients typically received 1 to 4 IPL treatments spaced 4 to 6 weeks apart. Each IPL session included MGX. Thirty-five charts had complete data for inclusion in analysis. We reviewed demographics, ocular histories, Standard Patient Evaluation of Eye Dryness 2 (SPEED2) symptom survey scores, slit-lamp examinations, and meibomian gland evaluations (MGE) at baseline and at each visit before IPL/MGX treatments. Results: The paired t test showed a significant (P < 0.0001) decrease in SPEED2 with IPL/MGX therapy. Of the 35 patients, 8 (23%) had a ≥50% decrease in SPEED2, 23 (66%) had a 1% to 49% decrease in SPEED2, 1 (3%) had no change in SPEED2, and 3 (9%) had an increase in SPEED2. The Paired t test showed a significant increase in MGE in the left eye but not in the right eye (OD P = 0.163 and OS P = 0.0002). Thirteen patients (37%) had improved MGE bilaterally. Eight patients (23%) had either a decrease in MGE bilaterally or a decrease in 1 eye with no change in the other eye. Conclusions: This retrospective analysis shows that the combination of IPL and MGX can significantly improve dry eye symptoms (in 89% of patients) and meibomian gland function (in 77% of patients in at least 1 eye).
Nasal absorption of tyrosine-linked model compounds.

The objective of this research is to explore the amino acid carrier-mediated pathway for nasal uptake of tyrosine-linked model compounds. These molecules were synthesized by ether linkage between the aromatic hydroxyl group of L-tyrosine and the terminal hydroxyl group of model compounds. An enzymatically stable linkage was utilized to link the model compounds to L-tyrosine so that the loss of the compound from perfusate signals absorption only without complication from metabolism. The rat in situ nasal perfusion technique was utilized in this investigation. The analyte concentrations remaining in the nasal perfusates were quantitated by a reversed-phase high-performance liquid chromatography. All L-tyrosine-linked model compounds were absorbed across the nasal mucosa (15-50% absorbed), and the extent of nasal absorption was concentration dependent. The apparent first-order rate constants of intact conjugate loss from the nasal perfusate were calculated. The Michaelis-Menten kinetic model was applied to fit the perfusion data. The Michaelis constant, K(m), and maximum or saturation rate of compound loss from the perfusate, V(max), were calculated from Lineweaver-Burk plots [e.g., K(m) and V(max) are 0.57 mM and 0.00515 micromol/min, respectively, for one of the model compounds, O-(4-carboxyphenylmethyl)-L-tyrosine]. The absorption characteristics of all these model compounds exhibited saturable kinetics, whereas their parent compounds, possessing different octanol-water partition coefficients, showed either no absorption or transport by passive diffusion. Nasal uptake of L-tyrosine conjugates was inhibited by L-tyrosine. The results from this study indicate that nasal absorption of L-tyrosine-linked compounds is probably through an amino acid transport system. Exploitation of the amino acid transport pathway may be utilized to improve nasal absorption of poorly permeable drugs.
Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing

Knowledge of the rate and nature of spontaneous mutation is fundamental to understanding evolutionary and molecular processes. In this report, we analyze spontaneous mutations accumulated over thousands of generations by wild-type Escherichia coli and a derivative defective in mismatch repair (MMR), the primary pathway for correcting replication errors. The major conclusions are (i) the mutation rate of a wild-type E. coli strain is ∼1 × 10−3 per genome per generation; (ii) mutations in the wild-type strain have the expected mutational bias for G:C > A:T mutations, but the bias changes to A:T > G:C mutations in the absence of MMR; (iii) during replication, A:T > G:C transitions preferentially occur with A templating the lagging strand and T templating the leading strand, whereas G:C > A:T transitions preferentially occur with C templating the lagging strand and G templating the leading strand; (iv) there is a strong bias for transition mutations to occur at 5′ApC3′/3′TpG5′ sites (where bases 5′A and 3′T are mutated) and, to a lesser extent, at 5′GpC3′/3′CpG5′ sites (where bases 5′G and 3′C are mutated); (v) although the rate of small (≤4 nt) insertions and deletions is high at repeat sequences, these events occur at only 1/10th the genomic rate of base-pair substitutions. MMR activity is genetically regulated, and bacteria isolated from nature often lack MMR capacity, suggesting that modulation of MMR can be adaptive. Thus, comparing results from the wild-type and MMR-defective strains may lead to a deeper understanding of factors that determine mutation rates and spectra, how these factors may differ among organisms, and how they may be shaped by environmental conditions.
Training Methods and Association with Worker Injury on Colorado Dairies

Abstract The goals of this study were to define the current methods for training entry-level workers on Colorado dairies, to measure the relationship of training methods to the incidence of work-related injuries on those operations, and to characterize the nature and cause of injuries reported by participating workers. Seventy-two workers from 15 dairies from Weld, Morgan, Larimer and Yuma counties volunteered to be interviewed privately in the language of their choice. Training methods were classified by content (task training versus safety training), method of delivery (on-the-job verbal instruction, on-the-job demonstration, on-the-job supervision, classroom-based, and / or reading materials); and instructor (supervisor and/or co-worker). The majority (84.7%) of interviewed workers were Mexican-born, Spanish-speaking males. Most workers reported the training to be a combination of verbal instruction, demonstration and supervision. Twenty-nine of 72 (40.3%) of all participants suffered at least one work-related injury in the 12 months prior to the interview. Entry-level safety training had no protective effect (P = 0.463) on the reported incidence of injury in dairy workers in the previous year. However, workers that received task-related training were four times less likely to suffer a work-related injury (P = 0.008). Training conducted by a co-worker also had a significant protective effect against work-related injuries (P = 0.028). Dairy workers identified direct contact with livestock or machinery as the main cause for work-related injuries, and most injuries occurred while medicating sick cows and milking.
A 6–10.6 GHz CMOS PA with common-gate as an input stage for UWB transmitters

The design of a 6.0–10.6 GHz UWB CMOS power amplifier (PA) for ultra-wideband (UWB) transmitters in TSMC 0.18-µm CMOS technology is presented. The UWB PA proposed in this paper employing a common gate (CG) amplifier as the input stage for wideband matching and the current-reused technique is used to save the power consumption and to enhance gain at the higher frequency. The shunt peaking inductors were used to improve gain flatness and to increase the total bandwidth of the circuit. The post-layout simulation results show that the input return loss (S11) was less than −8 dB, output return loss (S22) was less than −10 dBm, and average gain was approximately 11 dB over the frequencies ranges of interest. The input and output 1-dB compression point is −12 dBm and 0 dBm, respectively. Moreover, an excellent phase linearity property (group delay) of ±55.8 ps across the whole band was obtained with power consumption of 18 mW. The chip area is 0.77 mm2.
Automatic Segmentation and Shape-Based Classification of Retro-Reflective Traffic Signs from Mobile LiDAR Data

Recently, many studies have demonstrated the valid contribution of mobile laser scanning to road safety improvements, thus intense efforts have been made to implement automatic data processing using laser scanning data, with special emphasis on road object recognition. This study is focused on the detection and classification of retro-reflective vertical traffic signs according to their function (danger, give way, prohibition/obligation, and indication) from mobile laser scanning data by considering geometric and radiometric information, but without relying on trajectory data. The global strategy for segmentation involves the application of an optimized intensity threshold in order to segment the points that correspond to traffic sign panels. Next, contour recognition is performed for each sign using a linear regression model based on a raster image, which is generated for each cluster of points. The shape evaluation is motivated by the correspondence between contour shape and function of the traffic. The completeness of results for detection (92.11%) and classification (83.91%) demonstrates that this implementation is promising for the automatic detection and inventory analysis of traffic signs in road mapping applications. The efficiency rates are acceptable in urban areas, but our tests indicate that the detection and classification rates are more robust in road environments.
Comparison of Standing Sagittal Spinal Alignment in Asymptomatic Adolescents and Adults

Study Design. A retrospective examination of the spine radiographs of 88 asymptomatic adolescents was performed to analyze the indices of regional and segmental sagittal spinal alignment in relation to the sagittal vertical axis as determined by the C7 plumb line. Objectives. To determine the sagittal spinal alignment in asymptomatic adolescents and to correlate the sagittal vertical axis with the overall sagittal spinal balance and other indices of sagittal spinal alignment. In addition, to compare these results with previously established data for asymptomatic adults. Summary of Background Data. Previous studies of sagittal spinal alignment have included subjects encompassing a wide range of ages. A previous study at the authors' institution established normative data for sagittal spinal alignment in asymptomatic adults. No previous study has reported on the correlation between the sagittal vertical axis and other measurements of sagittal spinal alignment in asymptomatic adolescents. Methods. Measurements obtained from the standing lateral spine radiographs of 88 asymptomatic adolescents (age range, 10‐18 years) were collected and analyzed using statistical methods. These data were compared with previously established data for asymptomatic adults. Results. There was a striking similarity in regional thoracic kyphosis and lumbar lordosis between adolescents and adults. Despite having similar regional and segmental sagittal alignments, adolescents had a significantly more negative sagittal vertical axis (mean, −5.6 cm) than adults (mean, −3.2 cm; P = 0.0001). Also, unlike that in adults, the sagittal vertical axis in adolescents was not significantly correlated with the distal segmental lumbar lordosis. The sagittal vertical axis in adolescents was significantly correlated with the level of thoracic kyphosis and the distances from the thoracic apex, lumbar apex, and T12 to the C7 plumb line. Sacral inclination, which is a determinant of hip extension and standing pelvic rotation, was correlated with the lumbar apex and the total and segmental lordosis, except at L5‐S1. Conclusions. Asymptomatic adolescents tend to stand in greater negative sagittal spinal balance than asymptomatic adults, despite similar regional and segmental alignments in the thoracic and lumbar spine. The role of hip extension, spinopelvic axis rotation, and other sagittal alignment parameters in determining the sagittal vertical axis in adolescents warrants further study.