Miracle-dz/UniFAR-FTUs huggingface dataset:
ISOLATION, OPTIMIZATION AND PURIFICATION OF LIPASE FROM MYROIDES ODORATIMIMUS

Lipases (Triacylglycerol acylhydrolase) are industrially important enzymes that are mainly used in the food and pharmaceutical industry. Lipase enzymes are also used in food materials, detergent, medicine, cosmetics, leather, perfumery and other items. The enzyme was isolated from Myroides odoratimimus and optimized to different physiochemical characteristics, giving its optimum temperature 37°C, active over a wide range pH values (pH 8.2) and increase in substrate (Olive Oil) concentration that increases the activity of lipase. The enzyme lipase activity was stimulated by Ca +2 and Cu +2 , inhibited by BaCl2. All these characteristics are considered favorable for industrial processing. Lipase Activity has been measured by titrimetric, and colorimetric method, and was purified by CM-Cellulose, DEAE -Cellulose, Sephadex G-75 Chromatography, respectively; the purified enzyme was increased by gel filtration. For the protein determination (molecular weight) of our enzyme, we under went for SDS -PAGE, which showed approximately 40KDa. Zymography has been performed showing the maximum activity of the purified lipase, resulting in a clear zone of layer. Native Polyacrylamide Gel Electrophoresis (PAGE) was also performed, in which the gels were overlaid with the molten Chromogenic substrate(40°C), which was then allowed to solidify and incubated, depending on the amount of lipase, the activity was observed as yellow band over a pink background.
Efficacy and safety of pancreatic enzyme replacement therapy on exocrine pancreatic insufficiency: a meta-analysis

Background Pancreatic enzyme replacement therapy (PERT) is widely applied to patients with exocrine pancreatic insufficiency (EPI), but its effect and safety has not been quantified. Therefore we performed a meta-analysis to determine the efficacy and tolerance of PERT on patients with EPI. Materials and Methods PubMed, Medline, Cochrane library database, Evidence-based medicine/clinical trials published before December 2016 were searched by two independent reviewers to identify prospective randomized controlled trials (RCTs). Results Seven RCTs, randomizing a total of 282 patients, were filtrated and assessed qualitatively (Jadad score). PERT increased CFA (WMD: 26.56, 20.35 to 32.76, I2 = 79.6%, P < 0.001) compared with baseline, and CFA (WMD: 17.97, 12.61 to 23.34, I2 = 76.7%, P < 0.001) vs. placebo. Meanwhile, CNA, SFE, SNE and SW were significantly improved in PERT compared with baseline and placebo, with no statistical differences in adverse events. Subgroup analysis indicated that standard forms of PERT displayed more effectiveness with significantly decreased heterogeneity, and large sample size also reduced the heterogeneity to some degree. Conclusions PERT is demonstrated to be effective and tolerable in patients with EPI, especially using standard administration of PERT. Larger and higher quality studies on EPI are demanded to long-term effect of standard PERT treatment.
Effect of losartan on albuminuria, peripheral and autonomic neuropathy in normotensive microalbuminuric type 2 diabetics.

BACKGROUND Angiotensin converting enzyme (ACE) inhibitors are emerging as effective agents for preventing microvascular complications of diabetes. Losartan (angiotensin II antagonist) has an antihypertensive efficacy equivalent to ACE inhibitors, however its role in microvascular complications is not yet known. MATERIAL AND METHODS We studied the efficacy of losartan (50 mg once daily for 12 weeks) on albuminuria, peripheral and autonomic neuropathy in 25 normotensive microalbuminuric type 2 diabetics who were asymptomatic for neuropathy. RESULTS Mean age was 46.6 +/- 4.34 years with the average duration of diabetes being 8.1 +/- 1.54 years. Albuminuria improved significantly from 54 +/- 9.35 mg/L to 32.8 +/- 25 mg/L (Paired student's t-test, P=0.0005) after therapy. Autonomic neuropathy was observed in 64% while 76% had peripheral neuropathy; but there was no improvement with losartan. The duration of diabetes had a negative correlation with autonomic neuropathy. It also had a similar negative correlation with median and common peroneal nerve motor conduction velocities (Pearson's correlation coefficient, r = -0.53, P<0.01 and r = -0.56, P<0.01 respectively) implying that autonomic and peripheral neuropathy worsen as a diabetic ages. However, no correlation existed between albuminuria and autonomic or peripheral nerve function. CONCLUSION Autonomic and peripheral neuropathy are highly prevalent in normotensive microalbuminuric diabetic patients. Losartan remarkably improves albuminuria but a similar benefit in autonomic or peripheral neuropathy is not seen over 12 weeks. The future may see a defining role for losartan in microvascular complications in normotensive diabetics.
Interpretation of remotely sensed images in a context of multisensor fusion using a multispecialist architecture

A scene interpretation system is presented in a multisensor fusion context the application of which involves the interpretation of remote sensed images. The authors discuss how multisensor fusion is achieved, and derive the modeling problems for objects, scene, and strategy. The proposed multispecialist architecture takes into account the knowledge about sensors, the multiple viewing notion (shot), and the uncertainty and imprecision of models and data modeled with possibility theory. Generic models of objects are represented by concepts independent of sensors. Three kinds of specialists are present in the architecture: generic specialists (scene and conflict), semantic object specialists, and low level specialists. A blackboard structure with a centralized control is used. The interpreted scene is implemented as a matrix of pointers allowing easy conflict detection and spatial context verification. An interpretation system for SAR/SPOT sensor images is described, and an example of a scene interpretation is shown. >
Towards power consumption-delay tradeoff by workload allocation in cloud-fog computing

Fog computing, characterized by extending cloud computing to the edge of the network, has recently received considerable attention. The fog is not a substitute but a powerful complement to the cloud. It is worthy of studying the interplay and cooperation between the edge (fog) and the core (cloud). To address this issue, we study the tradeoff between power consumption and delay in a cloud-fog computing system. Specifically, we first mathematically formulate the workload allocation problem. After that, we develop an approximate solution to decompose the primal problem into three subproblems of corresponding subsystems, which can be independently solved. Finally, based on extensive simulations and numerical results, we show that by sacrificing modest computation resources to save communication bandwidth and reduce transmission latency, fog computing can significantly improve the performance of cloud computing.
Functional Characterization of Acinetobacter baumannii Lacking the RNA Chaperone Hfq

The RNA chaperone Hfq is involved in the riboregulation of diverse genes via small RNAs. Recent studies have demonstrated that Hfq contributes to the stress response and the virulence of several pathogens, and the roles of Hfq vary among bacterial species. Here, we attempted to elucidate the role of Hfq in Acinetobacter baumannii ATCC 17978. In the absence of hfq, A. baumannii exhibited retarded cell growth and was highly sensitive to environmental stress, including osmotic and oxidative pressure, pH, and temperature. Compared to the wild-type, the Hfq mutant had reduced outer membrane vesicles secretion and fimbriae production as visualized by atomic force microscopy. The absence of hfq reduced biofilm formation, airway epithelial cell adhesion and invasion, and survival in macrophage. Further, the hfq mutant induced significantly higher IL-8 levels in airway epithelial cells, which would promote bacterial clearance by the host. In addition to results similar to those reported for other bacteria, our findings demonstrate that Hfq is required in the regulation of the iron-acquisition system via downregulating the bauA and basD genes, the stress-related outer membrane proteins carO, A1S_0820, ompA, and nlpE, and the stress-related cytosolic proteins uspA and groEL. Our data indicate that Hfq plays a critical role in environmental adaptation and virulence in A. baumannii by modulating stress responses, surface architectures, and virulence factors. This study is the first to illustrate the functional role of Hfq in A. baumannii.
Human Resource Assignment and Role Representation Mechanism with the "Cascading Staff-Group Authoring" and "Relation/Situation" Model

We have previously reported the access control mechanism and audit strategy of the "patient-doctor relation and clinical situation at the point-of-care" model with multi-axial access control matrix (ACM). This mechanism overcomes the deficit of ACM in the aspect of data accessibility but does not resolve the representation of the staff's affiliate and/or plural membership in the complex real world. Care groups inside a department or inter-department clinical team plays significant clinical role but also spend great amount of time and money in the hospital. Therefore the impact of human resource assignment and cost of such stakeholders to the hospital management is huge, so that they should be accurately treated in the hospital information system. However multi-axial ACM has problems with the representation of staff groups due to static parameters such as department/license because staffs belong to a group rather temporarily and/or a medical staff may belong to plural groups. As a solution, we have designed and implemented "cascading staff-group authoring" method with "relation and situation" model and multi-axial ACM. In this mechanism, (i) a system administrator certifies "group chief certifying person" according to the request and authorization by the department director, (ii) the "group chief certifying person" certifies "group chief(s)", (iii) the "group chief" recruits its members from the medical staffs, and at the same time the "group chief" decides the profit distribution policy of this group. This will enable medical staff to access EMR according to the role he/she plays whether it is as a department staff or as a group member. This solution has worked successfully over the past few years. It provides end-users with a flexible and time-to-time staff-group authoring environment using a simple human-interfaced tool without security breach and without system administration cost. In addition, profit and cost distribution is clarified among departments and inter-departments groups.
Single-Vesicle Catecholamine Release Has Greater Quantal Content and Faster Kinetics in Chromaffin Cells from Hypertensive, as Compared with Normotensive, Rats

In a previous study performed in the intact adrenal gland (Lim et al., 2002), stimulation with acetylcholine (ACh) or high K+ concentrations (K+) produced greater catecholamine release in spontaneously hypertensive rats (SHR), as compared with normotensive animals. In this study, the time course of secretion was in the range of minutes. Hence, we do not know whether enhanced release is due to greater quantal content and/or distinct kinetics in SHRs and control animals. To get insight into the mechanism involved in such enhanced catecholamine secretory responses, we performed a single-vesicle release study in primary cultures of adrenal chromaffin cells, recorded with amperometry. Cells were stimulated with 2-s pulses of 1 mM ACh or 70 mM K+. The secretory responses to ACh or K+ pulses in SHR cells as compared with control cells had the following characteristics: 1) double number of secretory events, 2) 4-fold augmentation of total secretion, 3) cumulative secretion that saturated slowly, 4) 3-fold higher complex events with two to four superimposed spikes that may be explained by faster spike kinetics, 5) about 2- to 3-fold higher event frequency at earlier post stimulation periods, and 6) 2- to 5-fold higher quantal content of simple spikes. We conclude that SHR cells have faster and larger catecholamine release responses, explained by more vesicles ready to undergo exocytosis and greater quantal content of vesicles. This could have relevance to further understand the pathogenic mechanisms involved in the development of high blood pressure, as well as in the identification of new drug targets to treat hypertension.
Multiphase CT enterography evaluation of small-bowel vascular lesions.

OBJECTIVE By use of multiphase CT enterography (CTE), small-bowel vascular lesions associated with gastrointestinal bleeding can be classified into three categories--angioectasias, arterial lesions, and venous abnormalities--on the basis of common morphology and enhancement patterns. This article will review the unique patterns of enhancement and lesion morphology seen on multiphase CTE and how those findings enable detection and characterization of specific lesions in many cases. CONCLUSION Because of the high prevalence in nonbleeding patients and frequent multiplicity of angioectasias, determining the clinical benefit from their detection by multiphase CTE and endoscopy is problematic. Although arterial lesions are less commonly encountered clinically, their detection is critically important because of a high risk of life-threatening bleeding. Along with wireless capsule endoscopy and balloon-assisted endoscopy, multiphase CTE is a useful tool for the evaluation of patients with obscure gastrointestinal bleeding due to small-bowel vascular lesions.
Radiological survey of men exposed to asbestos in naval dockyards

Harries, P. G. , Mackenzie, F. A. F. , Sheers, G. , Kemp, J. H. , Oliver, T. P., and Wright, D. S. (1972). Brit. J. industr. Med.,29, 274-279. Radiological survey of men exposed to asbestos in naval dockyards. Asbestos related abnormalities were found in 3% of a 10% sample population in radiological surveys of the naval dockyards at Portsmouth, Chatham, and Rosyth. The prevalence of these abnormalities was related to the type of occupation and duration of exposure to asbestos. The results confirm the findings of an earlier survey at Devonport dockyard. No association between smoking, or the amount smoked, and the incidence of parenchymal or pleural disease due to asbestos was detected. Pleural abnormalities were found 10 times more frequently than parenchymal disease, and concern is felt over the uncertainty of the prognosis in men with pleural abnormalities, especially as 37 men have developed pleural mesothelioma at Devonport since 1965. More work is required to establish the true significance of pleural abnormalities caused by asbestos and to explore possible methods of treatment.
[Histological and morphological studies on the rhizomes of Curcuma].

OBJECTIVE To provide some new evidences for the identification of medicinal materials of Curcuma. METHOD Microscopic observation was made to characterize the rhizomes of Curcuma. RESULT AND CONCLUSION There were no obvious histological and morphological differences among the rhizomes of Curcuma. The distribution of oil cells and vascular bundles as well as the number and diameter of xylem vessels were considered to be the distinguishing features of their rhizomes.
A novel multistage genetic algorithm approach for solving Sudoku puzzle

Sudoku is a NP complete combinatorial number placement puzzle which has been solved using various algorithms including evolutionary algorithms. In this paper, we propose a multistage genetic algorithm (GA) for solving Sudoku. In this algorithm, the group table concept has been incorporated. This work progresses with a couple of cycles. In every cycle GA works for finding better solution. The each elements of the best solution in any particular cycle undergo through a multidirectional crosscheck validation process and finally selected subject to a probability. After each cycle, group table is updated depending on the chosen elements of the best solution in the previous cycle. This algorithm also comprises of new population generation, fitness assignment with more penalization, crossover and mutation operators etc. The results show that multistage GA is competitive with good successful rate for solving various Sudoku puzzles.
An Efficient and Provably Secure Certificateless Key Insulated Encryption with Applications to Mobile Internet

Certiﬁcateless encryption (CLE) alleviates the heavy cer-tiﬁcate management in traditional public key encryption and the key escrow problem in the ID-based encryption simultaneously. Current CLE schemes assumed that the user’s secret key is absolutely secure. Unfortunately, this assumption is too strong in case the CLE is deployed in the hostile setting and the leakage of the secret key is inevitable. In this paper, we present a new concept called a certiﬁcateless key insulated encryption scheme(CL-KIE). We argue that this is an important cryptographic primitive that can be used to achieve key-escrow free and key-exposure resilience. We also present an eﬃcient CL-KIE scheme based on bilinear pairing. After that, the security of our scheme is proved under the Bilinear Diﬃe-Hellman assumption in the random oracle model. Further, the potential applications of CL-KIE is also brieﬂy illustrated.
Mutation of the 3-Phosphoinositide-Dependent Protein Kinase 1 (PDK1) Substrate-Docking Site in the Developing Brain Causes Microcephaly with Abnormal Brain Morphogenesis Independently of Akt, Leading to Impaired Cognition and Disruptive Behaviors

ABSTRACT The phosphoinositide (PI) 3-kinase/Akt signaling pathway plays essential roles during neuronal development. 3-Phosphoinositide-dependent protein kinase 1 (PDK1) coordinates the PI 3-kinase signals by activating 23 kinases of the AGC family, including Akt. Phosphorylation of a conserved docking site in the substrate is a requisite for PDK1 to recognize, phosphorylate, and activate most of these kinases, with the exception of Akt. We exploited this differential mechanism of regulation by generating neuron-specific conditional knock-in mice expressing a mutant form of PDK1, L155E, in which the substrate-docking site binding motif, termed the PIF pocket, was disrupted. As a consequence, activation of all the PDK1 substrates tested except Akt was abolished. The mice exhibited microcephaly, altered cortical layering, and reduced circuitry, leading to cognitive deficits and exacerbated disruptive behavior combined with diminished motivation. The abnormal patterning of the adult brain arises from the reduced ability of the embryonic neurons to polarize and extend their axons, highlighting the essential roles that the PDK1 signaling beyond Akt plays in mediating the neuronal responses that regulate brain development.
Variable-length VLIW encoding for code size reduction in embedded processors

Very-long-instruction-word (VLIW) architectures are widely adopted in high-performance and low-power digital signal processors (DSP) due to their simplicity from extensive software optimizations. However, their poor code density (usually > 2× code size for a given application) and corresponding instruction accesses can overwhelm the energy savings on DSP datapaths. This paper presents variable-length VLIW (VL2IW), where the unused condition codes, operands/operand scope, and reduced range of immediate variables of TI C64x+ DSP are exploited to improve the code density. The static grouping and run-time dispersal schemes of the variable-length instructions in a VLIW packet are described. In our experiments, 21% code sizes are saved in average for MiBench kernels. The hardware overhead is only ∼5%.
Regulation of Secondary Cell Wall Development by Cortical Microtubules during Tracheary Element Differentiation in Arabidopsis Cell Suspensions1[w]

Cortical microtubules participate in the deposition of patterned secondary walls in tracheary element differentiation. In this study, we established a system to induce the differentiation of tracheary elements using a transgenic Arabidopsis (Arabidopsis thaliana) cell suspension stably expressing a green fluorescent protein-tubulin fusion protein. Approximately 30% of the cells differentiated into tracheary elements 96 h after culture in auxin-free media containing 1 μm brassinolide. With this differentiation system, we have been able to time-sequentially elucidate microtubule arrangement during secondary wall thickening. The development of secondary walls could be followed in living cells by staining with fluorescein-conjugated wheat germ agglutinin, and the three-dimensional structures of the secondary walls could be simultaneously analyzed. A single microtubule bundle first appeared beneath the narrow secondary wall and then developed into two separate bundles locating along both sides of the developing secondary wall. Microtubule inhibitors affected secondary wall thickening, suggesting that the pair of microtubule bundles adjacent to the secondary wall played a crucial role in the regulation of secondary wall development.
Immune Factors in Breast Milk and the Development of Atopic Disease

ABSTRACT Breast-feeding provides protection against infections and contains numerous factors that modulate and promote the development of the infant immune system. These factors include secretory IgA, antimicrobial proteins like CD14, cytokines, and fatty acids. Studies examining the role of breast-feeding in the development of allergic disease in infants demonstrate potentially protective as well as neutral or nonprotective effects, likely due to the heterogeneity in their study design. In this overview, we explore the potential role of immune factors in the breast milk, as well as selected probiotics, in the development of allergy.
Regulation of autoimmune diabetes by non‐islet‐specific T cells — a role for the glucocorticoid‐induced TNF receptor

Diabetogenic BDC2.5 CD4 T cells induce diabetes when injected into NOD.scid mice. However, when co‐transferred with the OVA‐specific DO11.10 CD4 T cells, BDC2.5 T cells failed to cause diabetes. This inhibition depended upon the stimulation of DO11.10 T cells only with soluble OVA, which skewed their differentiation to a Th2‐type pattern of cytokine secretion in vivo. However, in vivo neutralization of IL‐4, IL‐10 or TGF‐β using monoclonal antibodies did not prevent the inhibition whereas treatment with an antibody against the glucocorticoid‐induced TNF receptor abrogated the protection from disease. In the protected mice, the diabetogenic T cells could be isolated from their spleens and shown to transfer diabetes when injected into new NOD.scid recipients. Thus, the inhibition took place without the physical or functional elimination of the diabetogenic T cells.