Miracle-dz/UniFAR-FTUs huggingface dataset:
Posttraumatic Stress Disorder Symptom Clusters Predicting Substance Abuse in Adolescents

The majority of studies exploring the mental health disorders, post-traumatic stress disorder (PTSD) and substance use disorders (substance abuse (SA) and dependence), have shown high co-morbidity rates in adolescents, indicating a well-established relationship. However, only a few studies have attempted to examine the functional mechanisms believed to underlie this common form of co-morbidity, and no studies have been carried out in adolescent samples. Knowledge is thus lacking on how specific PTSD symptom clusters may account for the development of specific types of problematic substance use in adolescents. Therefore, we studied this issue in relation to alcohol abuse (AA) and drug abuse (DA) in a probability sample of Danish 15–18-year-olds (n = 1988) in the form of an online survey using self-report questionnaires following the Diagnostic and statistical manual of mental disorders, fourth edition [DSM-IV; APA. (1994). Washington, DC: Author]. After demographic and substance-related variables were cont.
Carnitine-acyltransferase system inhibition, cancer cell death, and prevention of myc-induced lymphomagenesis.

BACKGROUND The metabolic alterations of cancer cells represent an opportunity for developing selective antineoplastic treatments. We investigated the therapeutic potential of ST1326, an inhibitor of carnitine-palmitoyl transferase 1A (CPT1A), the rate-limiting enzyme for fatty acid (FA) import into mitochondria. METHODS ST1326 was tested on in vitro and in vivo models of Burkitt's lymphoma, in which c-myc, which drives cellular demand for FA metabolism, is highly overexpressed. We performed assays to evaluate the effect of ST1326 on proliferation, FA oxidation, and FA mitochondrial channeling in Raji cells. The therapeutic efficacy of ST1326 was tested by treating Eµ-myc mice (control: n = 29; treatment: n = 24 per group), an established model of c-myc-mediated lymphomagenesis. Experiments were performed on spleen-derived c-myc-overexpressing B cells to clarify the role of c-myc in conferring sensitivity to ST1326. Survival was evaluated with Kaplan-Meier analyses. All statistical tests were two-sided. RESULTS ST1326 blocked both long- and short-chain FA oxidation and showed a strong cytotoxic effect on Burkitt's lymphoma cells (on Raji cells at 72 hours: half maximal inhibitory concentration = 8.6 μM). ST1326 treatment induced massive cytoplasmic lipid accumulation, impairment of proper mitochondrial FA channeling, and reduced availability of cytosolic acetyl coenzyme A, a fundamental substrate for de novo lipogenesis. Moreover, treatment with ST1326 in Eµ-myc transgenic mice prevented tumor formation (P = .01), by selectively impairing the growth of spleen-derived primary B cells overexpressing c-myc (wild-type cells + ST1326 vs. Eµ-myc cells + ST1326: 99.75% vs. 57.5%, difference = 42.25, 95% confidence interval of difference = 14% to 70%; P = .01). CONCLUSIONS Our data indicate that it is possible to tackle c-myc-driven tumorigenesis by altering lipid metabolism and exploiting the neoplastic cell addiction to FA oxidation.
A continuous asymptotic tracking control strategy for uncertain multi-input nonlinear systems

In this paper, we present a novel continuous control mechanism that compensates for uncertainty in a class of multi-input nonlinear systems. The control strategy is based on limited assumptions on the structure of the system nonlinearities. A Lyapunov-based stability argument is employed to prove semi-global asymptotic tracking. The control mechanism has the interesting feature of "learning" the unknown system dynamics. For the sake of clarity, the proposed control design is initially presented for a first-order, single-input case. Using this result as a stepping stone, the design is then extended to higher-order, multi-input systems.
Linear auto-calibration for ground plane motion

Planar scenes would appear to be ideally suited for self-calibration because, by eliminating the problems of occlusion and parallax, high accuracy two-view relationships can be calculated without restricting motion to pure rotation. Unfortunately, the only monocular solutions so far devised involve costly nonlinear minimizations, which must be initialized with educated guesses for the calibration parameters. So far, this problem has been circumvented by using stereo or a known calibration object. In this work we show that when there is some control over the motion of the camera, a fast linear solution is available without these restrictions. For a camera undergoing a motion about a plane-normal rotation axis (typified for instance by a motion in the plane of the scene), the complex eigenvectors of a plane-induced homography are coincident with the circular points of the motion. Three such homographies provide sufficient information to solve for the image of the absolute conic (IAC), and therefore the calibration parameters. The required situation arises most commonly when the camera is viewing the ground plane, and either moving along it, or rotating about some vertical axis. We demonstrate a number of useful applications, and show the algorithm to be simple, fast, and accurate.
Depletion of Serum Response Factor by RNA Interference Mimics the Mitogenic Effects of Platelet Derived Growth Factor-BB in Vascular Smooth Muscle Cells

Promoters of many smooth muscle-specific genes (SM-genes) contain multiple CArG boxes, which represent a binding site for serum response factor (SRF). Transciptional control through these regions involves interactions with SRF and specific coactivators such as myocardin. We have previously reported that suppression of SM-gene expression by platelet derived growth factor (PDGF) is associated with redistribution of SRF, leading to lower intra-nuclear levels, and a reduction in SRF transactivation. To further assess the role of SRF depletion on VSMC phenotype, the current study used RNA interference (RNAi). Two SRF-specific sequences constructed as hairpins were stably expressed in rat VSMC. Clones expressing SRF RNAi had no detectable SRF expression by immunoblotting, and showed diminished levels of SM &agr;-actin protein and promoter activity. Unexpectedly, depletion of VSMC resulted in increased rates of proliferation and migration. Several genes whose expression is increased by PDGF stimulation, including c-Jun, were similarly induced in cells lacking SRF. Effects of SRF depletion were not attributable to altered PDGF receptor activity or alterations in activation of Akt. These data indicate that loss of SRF transactivation in VSMC, in this case through suppression via RNAi, induces biological responses similar to that seen with PDGF.
Voice characteristics conversion for HMM-based speech synthesis system

We describe an approach to voice characteristics conversion for an HMM-based text-to-speech synthesis system. Since this speech synthesis system uses phoneme HMMs as speech units, voice characteristics conversion is achieved by changing the HMM parameters appropriately. To transform the voice characteristics of synthesized speech to the target speaker, we applied the maximum a posteriori estimation and vector field smoothing (MAP/VFS) algorithm to the phoneme HMMs. Using 5 or 8 sentences as adaptation data, speech samples synthesized from a set of adapted tied triphone HMMs, which have approximately 2,000 distributions, are judged to be closer to the target speaker by 79.7% or 90.6%, respectively, in an ABX listening test.
A psychoanalytic perspective on organizational change

Focuses on organizational change in a “first wave” NHS trust hospital. Examines the experiences of a group of clinical directors at this hospital as they respond to external pressures to change, instigated by recent Government policy. Analyses qualitative data, collected via in‐depth interviews with the clinical directors, using psychoanalytic concepts and theory.
Dissemination of a Clone of Unusual Phenotype of Pandrug-Resistant Acinetobacter baumannii at a University Hospital in Taiwan

ABSTRACT From December 2002 to February 2003, 15 isolates of pandrug-resistant unidentified Acinetobacter species were recovered from seven patients treated on different wards or intensive care units. Both 16S-23S rRNA intergenic spacer PCR-restriction fragment length polymorphism profiles and sequence analysis of these isolates identified them as Acinetobacter baumannii. This pandrug-resistant A. baumannii strain with an unusual phenotype could persist in humans for long periods and was widely disseminated throughout the hospital.
Update on activities at the Universal Protein Resource (UniProt) in 2013

The mission of the Universal Protein Resource (UniProt) (http://www.uniprot.org) is to support biological research by providing a freely accessible, stable, comprehensive, fully classified, richly and accurately annotated protein sequence knowledgebase. It integrates, interprets and standardizes data from numerous resources to achieve the most comprehensive catalogue of protein sequences and functional annotation. UniProt comprises four major components, each optimized for different uses, the UniProt Archive, the UniProt Knowledgebase, the UniProt Reference Clusters and the UniProt Metagenomic and Environmental Sequence Database. UniProt is produced by the UniProt Consortium, which consists of groups from the European Bioinformatics Institute (EBI), the SIB Swiss Institute of Bioinformatics (SIB) and the Protein Information Resource (PIR). UniProt is updated and distributed every 4 weeks and can be accessed online for searches or downloads.
A SURVEY ON NEW CRYPTOGRAPHIC SYSTEM TECHNIQUES FOR DATA SHARING IN CLOUD STORAGE

S.Thivaharan Assistant Professor, Dept of Information Technology Kalaignar Karunanidhi Institute of Technology Coimbatore, India s.thivaharan@gmail.com. Abstract— Cryptography is the art and science of achieving security by encoding the message or data to make them unreadable. It is related to the aspects of network security such as privacy, reliability and accessibility of the data. A cryptosystem or cryptographic system is only the authenticated way in which user can access the encrypted data and decrypt it. The authenticated messages are shared between the authorized users. Security in cloud computing is a major concern. There are some cryptosystem techniques introduced in this security. First, Attribute Based Encryption is a public key encryption technique which is used to enable the access control over the encrypted data using qualified attributes. Second, Identity Based Encryption allows for a sender can encrypt a message to an identity without access to a public key certificate. Third, a new public key cryptosystem encrypt a message not only a public key but also under an identifier of the ciphertext classes.
Purification, properties, and immunotitration of hepatoma glutamine phosphoribosylpyrophosphate amidotransferase (amidophosphoribosyltransferase, EC 2.4.2.14).

Abstract Glutamine phosphoribosylpyrophosphate amidotransferase (amidophosphoribosyltransferase, EC 2.4.2.14) from normal rat liver and rapidly growing Morris hepatoma 3924A has been purified 1622- and 1224-fold, respectively. Acrylamide gel electrophoresis demonstrated that the purified amidotransferase from liver and hepatoma had the same mobility. Kinetic studies established that the purified enzymes from liver and hepatoma 3924A were indistinguishable on the basis of their pH optima, affinity to substrates, sensitivity to AMP inhibition, and immunological characteristics. Both enzymes have a broad pH range of activity from 6.5 to 8.5. The liver and hepatoma enzymes exhibited very similar apparent K m 9s for glutamine, 1.4 and 1.5 mm, and for phosphoribosylpyrophosphate, 0.57 and 0.64 mm, respectively. In the absence of nucleotides, both amidotransferases showed hyperbolic curves for phosphoribosylpyrophosphate. However, the addition of AMP or GMP yielded sigmoidal curves for phosphoribosylpyrophosphate. The concentration of AMP needed to inhibit 50% of the purified amidotransferase activity from normal liver and hepatoma was similar, 4.1 and 3.3 mm, respectively. The molecular weights of the amidotransferase from liver and hepatoma were estimated to be 190,000 to 215,000 and 188,000 to 205,000 by sucrose density gradient centrifugation and by Sephadex G-150 column gel filtration. Using the highly purified amidotransferase from liver and hepatoma 3924A, antibodies were produced in rabbits. Immunotitration studies showed that the increased enzyme activity observed in the hepatoma was due to an increase in the enzyme protein amount. These studies provide further evidence that in the neoplastic transformation a reprogramming of gene expression takes place, which is manifested in the emergence of increased concentrations of amidotransferase, which should provide an increased capacity for this initiating rate-limiting step in the de novo purine-biosynthetic pathway.
Myeloid Differentiation Factor-88 Plays a Crucial Role in the Pathogenesis of Coxsackievirus B3–Induced Myocarditis and Influences Type I Interferon Production

Background— Myeloid differentiation factor (MyD)-88 is a key adaptor protein that plays a major role in the innate immune pathway. How MyD88 may regulate host response in inflammatory heart disease is unknown. Methods and Results— We found that the cardiac protein level of MyD88 was significantly increased in the hearts of wild-type mice after exposure to Coxsackievirus B3 (CVB3). MyD88−/− mice showed a dramatic higher survival rate (86%) in contrast to the low survival (35%) in the MyD88+/+ mice after CVB3 infection (P<0.0001). Pathological examination showed a significant decrease of cardiac and pancreatic inflammation in the MyD88−/− mice. Viral concentrations in the hearts were significantly decreased in the MyD88−/− mice. Cardiac mRNA levels for interleukin (IL)-1&bgr;, tumor necrosis factor (TNF)-&agr;, interferon (IFN)-&ggr;, and IL-18 were significantly decreased in the MyD88−/− mice. Similarly, serum levels of T-helper 1 cytokines were significantly decreased in the MyD88−/− mice. In contrast, cardiac protein levels of the activated interferon regulatory factor (IRF)-3 and IFN-&bgr; were significantly increased in the MyD88−/− mice but not other usual upstream signals to IRF-3. The cardiac expression of coxsackie-adenoviral receptor and p56lck were also significantly decreased. Conclusions— MyD88 appears to be a key contributor to cardiac inflammation, mediating cytokine production and T-helper-1/2 cytokine balance, increasing coxsackie-adenoviral receptor and p56lck expression and viral titers after CVB3 exposure. Absence of MyD88 confers host protection possibly through novel direct activation of IRF-3 and IFN-&bgr;.
Comparative analysis of optimization techniques for optimizing the radio network parameters of next generation wireless mobile communication

One of the primary aims of Optimization is to hunt the best entity from the available set of choice without explicitly assessing them. A new design, efficient outcome and cost effective solution is the outcome of optimization. Radio network planning is the basic seed required for configuring the base station parameters to attain the desired Quality of Service. The tuning of the radio network parameters like power control parameter, radius, tilt, azimuth orientation, cost etc. is a composite task, as majority of parameters are interdependent on each other. Optimization of these radio network parameters is the solution for the effective deployment of the network. Conventional optimizing algorithms like non-linear programming, Steepest Ascent, Golden Search, Newton Raphson, Quadratic programming etc are local search techniques which may stuck at local optimum. These techniques do not work for multi-modal functions and leads to degraded outcome if input parameters are misconfigured. To overcome this condition, modern optimization techniques like Genetic Algorithm, Particle Swarm Optimization, Simulated Annealing and Taguchi's Method of Optimization have been discussed in this paper. These meta-heuristic search techniques have been compared based on the exploration of search space, performance characteristics and computational intricacy. It is shown that Taguchi's method of Optimization has a comparable performance in terms of less iterations and control outcome.
Fathers and intergenerational transmission in social context

This article takes an intergenerational lens to the study of fathers. It draws on evidence from two economic and social research council-funded intergenerational studies of fathers, one of which focused on four-generation British families and the other which included new migrant (Polish) fathers. The article suggests both patterns of change and continuity in fatherhood across the generations. It demonstrates how cultural forces and material conditions need to combine to facilitate change in fathers’ exercise of agency and how social class and the conditions of being a migrant shape fathers’ practices. It argues that in seeking to recast the public debate about parenting, it is necessary to penetrate below the discursive level of talk about parenting to examine the habitual nature of many family practices, an endeavour to which an intergenerational approach is well suited. This approach enabled us to tease out the horizontal pull of within-generation influences on fathers, the vertical pull of inheritance from older to younger generations and the material and cultural conditions of fathers’ current locations, all of which shape their practices. This analysis also alerted us to changes in conceptual language – not only from fatherhood to fathering – but also to the historical resilience of the concept of childcare as reserved largely for the role and practices of mothers.
Emotional Communicability in Music Therapy: Different Instruments for Different Emotions?

In many instances the music therapist is called upon to choose a suitable instrument to communicate or express a certain emotional tone. We have examined music therapists 'expression of basic emotions on a variety of instruments. Four therapists made improvisations on five different emotions using four different instruments to convey each emotion. Seventy-six subjects, some of whom were music therapists and some non-therapists, listened to each improvisation and decoded its emotional content. Results pointed to specific emotion-instrumental patterns. Anger was communicated best on the drum, fear on the drum and the piano, sadness with the voice and the kazoo, and happiness and calmness with the voice. Music therapists were better than non-therapists at correctly decoding the emotional content of the improvisations. A short emotional training markedly improved therapists' accuracy at decoding emotions. Implications For the practice and training of music therapists are discussed.
How Applicable is Your Evaluation Methods – Really? Analysis and Re-design of Evaluation Methods for Fun and Entertainment

This paper presents an approach to analyze and re-design evaluation methods. The domain explored in this paper is evaluation methods for evaluating fun and entertainment. However, the approach presented may be applied in other domains as well. The approach is conceptually described and two examples of processes where the approach were used in practice are further discussed. As the map of IT applications and digital media is continuously re-designed, there is a constant need of re-designing evaluation methods.
Draft Genome Sequence of Marine Streptomyces sp. Strain W007, Which Produces Angucyclinone Antibiotics with a Benz[a]anthracene Skeleton

ABSTRACT A series of angucyclinone antibiotics have been isolated from marine Streptomyces sp. strain W007 and identified. Here, a draft genome sequence of Streptomyces sp. W007 is presented. The genome contains an intact biosynthetic gene cluster for angucyclinone antibiotics, which provides insight into the combinatorial biosynthesis of angucyclinone antibiotics produced by marine streptomycetes.
Education for Enterprise

Agreement with Napoleon's suggestion that Britain was a nation of shopkeepers has not, in itself, brought about an increased consciousness in our educational system that a knowledge of the process of wealth‐creation is a pre‐requisite for the young school‐leaver. In recent years, however, it has at last become respectable to be associated with vocational education. Statements by the Department of Education & Science, the activities of the Manpower Services Commission, and curriculum innovations such as TVEI and CPVE have highlighted the potential of vocational vehicles in the work of secondary schools in particular. Tragically many curriculum changes have come about as a result of the plague of unemployment which has beset the nation; and educational thinkers and developers have been forced into a corner, there to ponder on the activities of either the young unemployed or the potentially unemployed. Sadly, the development of Education for Enterprise in Durham University Business School over the last two years was born of an unemployment disaster.
Exploring the performance of ROS2

Middleware for robotics development must meet demanding requirements in real-time distributed embedded systems. The Robot Operating System (ROS), open-source middleware, has been widely used for robotics applications. However, the ROS is not suitable for real-time embedded systems because it does not satisfy real-time requirements and only runs on a few OSs. To address this problem, ROS1 will undergo a significant upgrade to ROS2 by utilizing the Data Distribution Service (DDS). DDS is suitable for real-time distributed embedded systems due to its various transport configurations (e.g., deadline and fault-tolerance) and scalability. ROS2 must convert data for DDS and abstract DDS from its users; however, this incurs additional overhead, which is examined in this study. Transport latencies between ROS2 nodes vary depending on the use cases, data size, configurations, and DDS vendors. We conduct proof of concept for DDS approach to ROS and arrange DDS characteristic and guidelines from various evaluations. By highlighting the DDS capabilities, we explore and evaluate the potential and constraints of DDS and ROS2.
The Notch Pathway Intermediate HES-1 Silences CD4 Gene Expression

ABSTRACT We have previously identified a transcriptional silencer that is critical for proper expression of the CD4 gene during T-cell development. Here we report that the Hairy/Enhancer of Split homologue HES-1, a transcription factor in the lin12/Notch signaling pathway, binds to an important functional site in the CD4 silencer. Overexpression of HES-1 leads to the silencer site-dependent repression of CD4 promoter and enhancer function as well as the downregulation of endogenous CD4 expression in CD4+ CD8−TH cells. Interestingly, overexpression of an activated form of Notch1 (NotchIC) leads to the repression of CD4 promoter and enhancer function both in the presence and absence of the silencer. NotchIC-mediated CD4 silencer function is not affected by the deletion of the HES-1-binding site, indicating that multiple factors binding to CD4 transcriptional control elements are responsive to signaling from this pathway, including other silencer-binding factors. Taken together, these data are consistent with the hypothesis that the lin12/Notch signaling pathway is important in thymic development and provide a molecular mechanism via the control of CD4 gene expression in which the lin12/Notch pathway affects T-cell developmental fate.