Miracle-dz/UniFAR-FTUs huggingface dataset:
Functionally significant allelic variation in myosin light chain composition in a tropical cichlid

Single fast muscle fibres in the tropical fish Oreochromis andersonii were found to contain two myosin light chains (LC1s; LC1f1* or LC1f2*). Breeding experiments confirmed that the different LC1s were of allelic origin and their inheritance patterns conformed to Mendelian expectations (1:2:1). The LC1s differed in apparent relative molecular mass by 800­900. No other differences in myosin subunits were found between the LC1 genotypes. The molar ratios of LC3:LC1(total) in the fast muscle of O. andersonii homozygous for LC1f1* or LC1f2* and heterozygous for both alleles were 2.0:1, 2.1:1 and 2.2:1, respectively, as determined by capillary electrophoresis. The maximum contraction velocity (Vmax) of single skinned muscle fibres was determined at 20 °C by the slack-test method. Vmax values (fibre lengths s-1) for fast muscle fibres from O. andersonii which were homozygous for either LC1f2* or LC1f1* were 5.3 and 3.3, respectively, compared with 3.8 when both alleles were present. Crosses between Oreochromis niloticus and O. andersonii produced F1 hybrids which were heterozygous for either LC1n/LC1f1* or LC1n/LC1f2*, where LC1n is the myosin light chain for O. niloticus. The distribution of myosin light chain genotypes in hybrid offspring was not significantly different from the expected Mendelian 1:1 ratio (47 %: 53 %). The Vmax (fibre lengths s-1) of muscle fibres containing LC1f2* from hybrid Oreochromis was 4.3 compared with 3.1 for the LC1f1* genotype. The results are consistent with a functionally significant allelic variation in myosin LC1 in fast muscle fibres from O. andersonii which is also expressed in hybrid genotypes.
Applying linguistics in making professional practice re-visible

In their introduction to the first issue of the relaunched Journal of Applied Linguistics and Professional Practice (JALPP), Sarangi and Candlin (2010) give two readings to the conjunction of ‘applied linguistics’ and ‘professional practice’ in the title, signalling two complementary agendas in applied linguistics research. The first agenda is to extend the study of language and communication to a wider range of professional contexts, i.e. to respecify the field of applied linguistics; the second is to contribute to the transformation and recontextualization of the professional practices of applied linguists in advancing knowledge and providing professional judgement. Following this lead, this special issue presents six papers which draw inspiration from Hallidayan scholarship in Systemic Functional Linguistics (SFL) to analyse, interpret and make sense of professional practices across a range of sites and from a variety of interdisciplinary perspectives, and in this introduction we set out and reflect on the appliability (Halliday 2007) of SFL as a mediational means in such research settings. In approaching this task, our first consideration was to ask what, beyond their use of a common theoretical/descriptive framework, unites the papers in this special issue? And perhaps the best answer to that is that each of the
Wave analysis of aperture field distribution for probe-fed radial line planar antennas

Probe-fed radial line planar antennas (RLPAs) have high efficiency, because the excitation probes of the radiating elements can be fed by a standing wave in the radial line with a short-circuited termination of its end. We propose a method for the wave analysis of a probe-fed RLPA. Good correspondence has been confirmed between measured and calculated values on the aperture field distribution. By observing the parameter dependence of the feeding network, a method is proposed to achieve a uniform aperture field distribution. The method is efficient for general geometries of probe-fed RLPAs.
Community-Owned Fiber Networks: Value Leaders in America

Our examination of advertised prices shows that community-owned ﬁber-to-the-home (FTTH) networks in the United States generally charge less for entry-level broadband service than do competing private providers, and don’t use initial low “teaser” rates that sharply rise months later. We also found that Comcast varies its pricing by region. Our study was constrained by the lack of standardization in Internet service offerings and a shortage of available data on broadband pricing in the United States. The U.S
Involvement of Poly(ADP-ribose) Polymerase-1 and XRCC1/DNA Ligase III in an Alternative Route for DNA Double-strand Breaks Rejoining*

The efficient repair of DNA double-strand breaks (DSBs) is critical for the maintenance of genomic integrity. In mammalian cells, the nonhomologous end-joining process that represents the predominant repair pathway relies on the DNA-dependent protein kinase (DNA-PK) and the XRCC4-DNA ligase IV complex. Nonetheless, several in vitro and in vivo results indicate that mammalian cells use more than a single end-joining mechanism. While searching for a DNA-PK-independent end-joining activity, we found that the pretreatment of DNA-PK-proficient and -deficient rodent cells with an inhibitor of the poly(ADP-ribose) polymerase-1 enzyme (PARP-1) led to increased cytotoxicity of the highly efficient DNA double-strand breaking compound calicheamicin γ1. In addition, the repair kinetics of the DSBs induced by calicheamicin γ1 was delayed both in PARP-1-proficient cells pretreated with the PARP-1 inhibitor and in PARP-1-deficient cells. In order to get new insights into the mechanism of an alternative route for DSBs repair, we have established a new synapsis and end-joining two-step assay in vitro, operating on DSBs with either nuclear protein extracts or recombinant proteins. We found an end-joining activity independent of the DNA-PK/XRCC4-ligase IV complex but that actually required a novel synapsis activity of PARP-1 and the ligation activity of the XRCC1-DNA ligase III complex, proteins otherwise involved in the base excision repair pathway. Taken together, these results strongly suggest that a PARP-1-dependent DSBs end-joining activity may exist in mammalian cells. We propose that this mechanism could act as an alternative route of DSBs repair that complements the DNA-PK/XRCC4/ligase IV-dependent nonhomologous end-joining.
Le management des systèmes PLM (product lifecycle management): Un agenda de recherche

Le product lifecycle management (PLM) est l’une de ces grandes vagues de réorientation stratégique des processus organisationnels permise par l’évolution des technologies de l’information et de la communication (telle que le customer relationship management (CRM) ou le supply-chain management (SCM)). L’objectif du PLM est la constitution d’un environnement informationnel intégré sur les produits de l’entreprise, afin de soutenir notamment la prise de décision lors de la conception et lors de la modification technique des produits. La recherche académique est aujourd’hui principalement axée sur les problématiques techniques de ces systèmes. Notre compréhension des dimensions organisationnelles et managériales de ces systèmes est encore faible. Le potentiel d’amélioration des décisions de conception permis par ces évolutions techniques et applicatives semble être encore largement méconnu. Dans cet article, nous passons en revue la littérature sur le PLM et nous proposons 14 voies de recherche sur les questions managériales de ces systèmes PLM. Nous espérons ainsi inciter certains de nos collègues à inscrire leurs travaux au sein de ces thématiques de recherche.
Self-rated health as an indicator of ideal cardiovascular health among working-aged women

Abstract Objective: The aim of this study was to assess the relationship between self-rated health (SRH) and the American Heart Association’s (AHA) concept of ideal cardiovascular health (CVH) among female municipal employees. Studies about the association are scarce, even though AHA recommends to use SRH status surveys in clinical practice and research settings of CVH. Design: Cross-sectional study conducted in 2014. Setting: SRH was assessed with a one-item question and the data of seven ideal CVH measurements (nonsmoking, body mass index <25.0 kg/m2, healthy diet, physical activity at goal, blood pressure <120/80 mmHg, cholesterol <5.18 mmol/l and glucose (HbA1c < 6.0%)) was gathered with a physical examination, laboratory tests, medical history and self-administrated questionnaires. Subjects: A total of 725 female subjects from 10 work units of the city of Pori, Finland. Main outcome measures: SRH and ideal CVH. Results: Of the study subjects, 28.8% reported ill-health (poor/fair SRH). The sum of ideal CVH metrics was positively associated with good SRH driven by favorable health behaviors (nonsmoking, normal body mass index, healthy diet and physical activity). A linear decrease in the prevalence of 0–2 ideal CVH metrics, and a linear increase in 5–7 metrics was associated with better SRH. Nonsmoking and normal weight were the most potent indicators of good SRH in multivariate analysis. Conclusions: Most of the subjects with 0–3 of the seven CVH metrics at ideal level were dissatisfied with their health. Since unhealthy lifestyle factors accumulate mainly to people feeling ill-health, the value of SRH is worth recognizing especially in primary health care.
Evaluating the capacity of a virtual r&d community of practice : The case of ALSTOM power hydro. (Comment organiser la pérennisation et le partage des connaissances dans un environnement international entre le centre de technologie et les bureaux d'études ?)

In this dissertation, we explore the potential of a forum to support collaboration and knowledge sharing among Virtual Communities of practice. We thus propose a coding scheme based on the Rainbow model and test it in order to analyze the content of two forums of R&D VcoP. We demonstrate that a forum supports asynchronous argumentative activities and thus enhances global collaboration and knowledge sharing among R&D VcoP members. We then propose an enriched model based on the work of Line Dube and tested it to characterize the R&D VcoP studied. We prove that the community configuration has a direct impact on the online dynamic of the community. We point out the main factors that play a key role in fostering online collaboration and knowledge sharing between R&D Virtual community members.
Design of a Novel Auto-Rotating UAV Platform for Underground Mine Cavity Surveying

The purpose of this paper is to investigate the potential for use of UAVs in underground mines and present a prototype design for a novel autorotating UAV platform for underground 3D data collection. The mining industry has recently shown increased interest in the use of unmanned aerial vehicles (UAVs) to assist in everyday operations [20, 11, 4]. Above ground, small UAVs are in some cases a more efficient, inexpensive, and safer alternative to manned aircrafts currently used for photography, inspection and security [6, 10]. For example, attaching camera, infrared and LiDAR payloads, UAVs can provide a low-cost method of obtaining highly accurate 3D photogrammetric data and aerial photography. UAVs are now commonly used in open pit mining operations for applications that include stockpile surveying, 3D pit modelling, facilities management, accident reporting, progress monitoring, and environmental assessment [11]. There are numerous companies offering UAV services for mining applications; e.g., UAV Geomatics, Leica Geosystems, Sky Futures, SenseFly and FlyTerra, to name just a few. Common services include stockpile and open pit volume computations, environmental assessment, aerial mapping and photogrammetry. Although the aforementioned services are those most commonly provided by UAV surveying companies, they may also add value to other daily mining operations. For instance, SenseFly offers pre and post-blast monitoring in order to identify the presence of misfires and wall damage. This data can also be used to reconcile the blast results with expected results. GEM Systems offers a UAV equipped with a magnetometer for mineral exploration surveys. Companies such as Barrick have even used UAVs for solids modelling at tailings dams and stability monitoring [11].
BIOEMULSIFIER PRODUCTION AND MOLECULAR DETECTION OF PSEUDOMONAS SPP. ISOLATED FROM HYDROCARBON CONTAMINATED SOIL

The soil is the complex mixture and harbors different kinds of contaminants. Petroleum-derived hydrocarbons are among the most persistent soil contaminants which can be degraded by microorganisms by producing biosurfactant and increase bioavailability of these pollutants. Some microorganisms can degrade these hydrocarbons and produce surface active compounds. Present study was focused on bioemulsifier producing Pseudomonas aeruginosa isolated from hydrocarbon contaminated soil with ability to utilize hydrocarbon. From 30 hydrocarbon contaminated soil samples, six strains of Pseudomonas spp. were isolated and its ability to produce stable oil water emulsion with different vegetable and petroleum oil was determined. The isolated strains of Pseudomonas sp. were identified by 16S rRNA sequencing and these were screened for ability to produce high molecular weight bioemulsifier by conventional methods by measuring reduction in surface tension, oil spreading ability and emulsification index. The study reveals that the isolates have ability to utilize as well as produce low and high molecular weight bioemulsifier when cultivated on oil containing medium.
Genomic Programming of Human Neonatal Dendritic Cells in Congenital Systemic and In Vitro Cytomegalovirus Infection Reveal Plastic and Robust Immune Pathway Biology Responses

Neonates and especially premature infants are highly susceptible to infection but still can have a remarkable resilience that is poorly understood. The view that neonates have an incomplete or deficient immune system is changing. Human neonatal studies are challenging, and elucidating host protective responses and underlying cognate pathway biology, in the context of viral infection in early life, remains to be fully explored. In both resource rich and poor settings, human cytomegalovirus (HCMV) is the most common cause of congenital infection. By using unbiased systems analyses of transcriptomic resources for HCMV neonatal infection, we find the systemic response of a preterm congenital HCMV infection, involves a focused IFN regulatory response associated with dendritic cells. Further analysis of transcriptional-programming of neonatal dendritic cells in response to HCMV infection in culture revealed an early dominant IFN-chemokine regulatory subnetworks, and at later times the plasticity of pathways implicated in cell-cycle control and lipid metabolism. Further, we identify previously unknown suppressed networks associated with infection, including a select group of GPCRs. Functional siRNA viral growth screen targeting 516-GPCRs and subsequent validation identified novel GPCR-dependent antiviral (ADORA1) and proviral (GPR146, RGS16, PTAFR, SCTR, GPR84, GPR85, NMUR2, FZ10, RDS, CCL17, and SORT1) roles. By contrast a gene family cluster of protocadherins is significantly differentially induced in neonatal cells, suggestive of possible immunomodulatory roles. Unexpectedly, programming responses of adult and neonatal dendritic cells, upon HCMV infection, demonstrated comparable quantitative and qualitative responses showing that functionally, neonatal dendritic cell are not overly compromised. However, a delay in responses of neonatal cells for IFN subnetworks in comparison with adult-derived cells are notable, suggestive of subtle plasticity differences. These findings support a set-point control mechanism rather than immaturity for explaining not only neonatal susceptibility but also resilience to infection. In summary, our findings show that neonatal HCMV infection leads to a highly plastic and functional robust programming of dendritic cells in vivo and in vitro. In comparison with adults, a minimal number of subtle quantitative and temporal differences may contribute to variability in host susceptibility and resilience, in a context dependent manner.
From foundations to frontiers: the status and perception of formulation teaching in Canadian English-speaking psychiatry residency programs

Aim of the study: Formulation is considered a key competence that should be taught during psychiatry training in residency. The scientific literature indicates shortcomings in teaching this clinical skill such as a lack of standardization and clear guidelines. The main objective of this research was to examine psychiatry residents’ perception regarding teaching of formulation in Canadian psychiatry residency programs.
The Korean Hearing in Noise Test

Korean is a language in the Altaic family, which includes Turkic, Mongolic, and Tungus-Manchu languages. The Korean language has 19 consonants and 9 different vowels with three series of stops and affricates: plain, aspirated, and glottalized (Lee et al., 2006). Although Korean is the official language of Korea and spoken by more than 72 million people including residents in China, the United States, Japan, Kazakhstan, and Uzbekistan, there are no standardized speech assessment materials for assessing functional hearing ability. The development of the Korean HINT system, which will enable inter-institutional and cross-language comparative studies, is described in this paper. Sentences were sampled, used in normal conversation without extensive memory function. Percent intelligibility of the sentences was measured at three S/N ratios of 7 dB, 4 dB, and 2 dB, with noise fixed at 65 dBA. After equalization of sentence difficulty, twenty-four 10-sentence lists were formed. Each sentence had an average of 3.2 0.6 (SD) words and 9.2 1.1 (SD) syllables. There were a total of 4814 phonemes in the entire twenty-four 10-sentence lists.
The Tuberous Sclerosis 2 Gene Product, Tuberin, Functions as a Rab5 GTPase Activating Protein (GAP) in Modulating Endocytosis*

The tuberous sclerosis complex 2 (TSC2) is a tumor suppressor gene that plays a causative role in the autosomal dominant syndrome of tuberous sclerosis. The latter is characterized by the development of hamartomas and occasional malignancies. Expression of the wild-type gene in TSC2 mutant tumor cells inhibits proliferation and tumorigenicity. This “suppressor” activity is encoded by functional domain(s) in the C terminus that contains homology to Rap1GAP. Using a yeast two-hybrid assay to identify proteins that interact with the C-terminal domain of tuberin, the product of TSC2, a cytosolic factor, rabaptin-5, was found to associate with a distinct domain lying adjacent to the TSC2 GAP homology region. Rabaptin-5 also binds the active form of GTPase Rab5. Immune complexes of native tuberin, as well as recombinant protein, possessed activity to stimulate GTP hydrolysis of Rab5. Tuberin GAP activity was specific for Rab5 and showed no cross-reactivity with Rab3a or Rab6. Cells lacking tuberin possessed minimal Rab5GAP activity and were associated with an increased uptake of horseradish peroxidase. Re-expression of tuberin in TSC2 mutant cells reduced the rate of fluid-phase endocytosis. These findings suggest that tuberin functions as a Rab5GAP in vivo to negatively regulate Rab5-GTP activity in endocytosis.
Necrolytic Migratory Erythema: First Symptom of a Glucagonoma A Case Report

Abstract Necrolytic migratory erythema (NME) is a rare skin condition consisting of migrating areas of erythema with blisters that heal with hyperpigmentation. NME is a pathognomonic feature of a glucagonoma. When confronted with a xerosis cutis one should always exclude the possibility of a NME caused by a glucagonoma as demonstrated in this case report where the rash was wrongly attributed to an acrodermatitis enteropathica due to zinc deficiency. We describe a case of a glucagonoma with its classic features, diagnosis and treatment.
Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells

We have cloned the human genomic DNA and the corresponding cDNA for the gene which complements the mutation of tsBN51, a temperature-sensitive (Ts) cell cycle mutant of BHK cells which is blocked in G1 at the nonpermissive temperature. After transfecting human DNA into TsBN51 cells and selecting for growth at 39.5 degrees C, Ts+ transformants were identified by their content of human AluI repetitive DNA sequences. Following two additional rounds of transfection, a genomic library was constructed from a tertiary Ts+ transformant and a recombinant phage containing the complementing gene isolated by screening for human AluI sequences. A genomic probe from this clone recognized a 2-kilobase mRNA in human and tertiary transformant cell lines, and this probe was used to isolate a biologically active cDNA from the Okayama-Berg cDNA expression library. Sequencing of this cDNA revealed a single open reading frame encoding a polypeptide of 395 amino acids. The deduced BN51 gene product has a high proportion of acidic and basic amino acids which are clustered in four hydrophilic domains spaced at 60- to 80-amino-acid intervals. These domains have strong sequence homology to each other. Thus, the tsBN51 protein consists of periodic repetitive clusters of acidic and basic amino acids.
Multiprocessor Interconnection Networks with Small tightness

Homogeneous multiprocessor systems are usually modelled by undirected graphs. Vertices of these graphs represent the processors, while edges denote the connection links between adjacent processors. Let G be a graph with diameter D, maximum vertex degree Δ, the largest eigenvalue λ1 and m distinct eigenvalues. The products mΔ and (D+1)λ1 are called the tightness of G of the first and second type, respectively. In recent literature it was suggested that graphs with a small tightness of the first type are good models for the multiprocessor interconnection networks. In a previous paper we studied these and some other types of tightness and some related graph invariants and demonstrated their usefulness in the analysis of multiprocessor interconnection networks. We proved that the number of connected graphs with a bounded tightness is finite. In this paper we determine explicitly graphs with tightness values not exceeding 9. There are 69 such graphs and they contain up to 10 vertices. In addition we identify graphs with minimal tightness values when the number of vertices is n = 2,…, 10.
Differential responses of epithelial Sertoli cells of the rat testis to Toll-like receptor 2 and 4 ligands: Implications for studies of testicular inflammation using bacterial lipopolysaccharides

The relative contribution of epithelial Sertoli cells in response to bacterial infection of the testis remains poorly characterised, since studies on inflammatory properties of these cells have invariably used unpurified lipopolysaccharide (LPS) preparations contaminated with bacterial lipopeptides. Consequently, isolated rat Sertoli cells were stimulated with either unextracted or phenol re-extracted LPS, and analysed for Toll-like receptor (TLR) 4, TLR2 and inflammatory cytokine gene expression by quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of TLR4 and its co-receptor protein myeloid differentiation (MD) 2 in Sertoli cells and testicular macrophages were similar, but Sertoli cells displayed low basal or LPS-induced expression of the TLR4 accessory protein, CD14. In Sertoli cells, unextracted LPS produced cytokine responses which were considerably greater in magnitude and duration compared with their response to purified LPS. Sertoli cells also responded to the synthetic lipopeptide, Pam3Cys (a TLR2 ligand) with a similar pattern of prolonged gene expression. Sertoli cells were more than 10-fold less sensitive to purified LPS than macrophages, but expressed similar levels of interleukin (IL)-1α and IL-6, and much greater levels of the immunoregulatory cytokine activin A, when maximally stimulated. These data demonstrate that Sertoli cells display differential cytokine responses to bacterial stimuli, mediated by both TLR2 and TLR4, that are distinct from those of testicular macrophages.