Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification and Characterization of Mycobacterial Proteins Differentially Expressed under Standing and Shaking Culture Conditions, Including Rv2623 from a Novel Class of Putative ATP-Binding Proteins

ABSTRACT The environmental signals that affect gene regulation inMycobacterium tuberculosis remain largely unknown despite their importance to tuberculosis pathogenesis. Other work has shown that several promoters, including acr (also known as hspX) (α-crystallin homolog), are upregulated in shallow standing cultures compared with constantly shaking cultures. Each of these promoters is also induced to a similar extent within macrophages. The present study used two-dimensional gel electrophoresis and mass spectrometry to further characterize differences in mycobacterial protein expression during growth under standing and shaking culture conditions. Metabolic labeling ofM. bovis BCG showed that at least 45 proteins were differentially expressed under standing and shaking culture conditions. Rv2623, CysA2-CysA3, Gap, and Acr were identified from each of four spots or gel bands that were specifically increased in bacteria from standing cultures. An additional standing-induced spot contained two comigrating proteins, GlcB and KatG. The greatest induction was observed with Rv2623, a 32-kDa protein of unknown function that was strongly expressed under standing conditions and absent in shaking cultures. Analysis using PROBE, a multiple sequence alignment and database mining tool, classified M. tuberculosis Rv2623 as a member of a novel class of ATP-binding proteins that may be involved in M. tuberculosis's response to environmental signals. These studies demonstrate the power of combined proteomic and computational approaches and demonstrate that subtle differences in bacterial culture conditions may have important implications for the study of gene expression in mycobacteria.
Gaussian Process Reinforcement Learning for Fast Opportunistic Spectrum Access

Opportunistic spectrum access (OSA) is envisioned to support the spectrum demand of future-generation wireless networks. The majority of existing work assumed independent primary channels with the knowledge of network dynamics. However, the channels are usually correlated and network dynamics is unknown a-priori. This entails a great challenge on the sensing policy design for spectrum opportunity tracking, and the conventional partially observable Markov decision process (POMDP) formulation with model-based solutions are generally inapplicable. In this paper, we take a different approach, and formulate the sensing policy design as a time-series POMDP from a model-free perspective. To solve this time-series POMDP, we propose a novel Gaussian process reinforcement learning (GPRL) based solution. It achieves accurate channel selection and a fast learning rate. In essence, GP is embedded in RL as a Q-function approximator to efficiently utilize the past learning experience. A novel kernel function is first tailor designed to measure the correlation of time-series spectrum data. Then a covariance-based exploration strategy is developed to enable a proactive exploration for better policy learning. Finally, for GPRL to adapt to multichannel sensing, we propose a novel action-trimming method to reduce the computational cost. Our simulation results show that the designed sensing policy outperforms existing ones, and can obtain a near-optimal performance within a short learning phase.
Expression of symbiotic genes of Rhizobium japonicum USDA 191 in other rhizobia

A 200-megadalton plasmid was mobilized from Rhizobium japonicum USDA 191 to other Rhizobium strains either that cannot nodulate soybeans or that form Fix- nodules on certain cultivars. The symbiotic properties of the transconjugants indicate that both soybean specificity for nodulation and cultivar specificity for nitrogen fixation are plasmid encoded.
Interlaboratory and intralaboratory comparisons of indirect immunofluorescence assays for serodiagnosis of Lyme disease

A conventional indirect immunofluorescence assay (IFA) and an anticomplement indirect immunofluorescence assay (ACIF) for detecting serum antibodies to Borrelia burgdorferi in humans were evaluated during a prevalence survey in northern California. Sera obtained from 119 current or former residents of an area in which Lyme disease is endemic were split and tested by the IFA in two laboratories and the ACIF in a third. The seropositivity rate ranged from 15 to 20% with 88 to 93% agreement among laboratories. Interlaboratory agreement was statistically highly significant in each of the three pairwise comparisons and was positively associated with clinical manifestations of Lyme disease. Intralaboratory agreement ranged from 93 to 96% in two laboratories and was also statistically highly significant. Immunoblotting confirmed 100 of 101 of the nondiscrepant immunofluorescence test results and likewise was positively correlated with the degree of interlaboratory agreement. The ACIF was found to be a highly specific test (100% specificity) with a much lower cutoff titer (1:8) than the conventional IFA (determined to be 1:128 or 1:256 in two laboratories) for detecting antibodies to B. burgdorferi. It also appeared to be more sensitive (80 versus 68%) than the IFA as determined by comparative immunoblotting, though the absolute sensitivity of the ACIF for serodiagnosis of early Lyme disease has yet to be determined. Significant serologic cross-reactivity was demonstrated between B. burgdorferi, Borrelia coriaceae, and Borrelia hermsii by the IFA, which may confound spirochetal serosurveys in California where all three spirochetes are known to coexist.
Alzheimer’s Diseases Detection by Using Deep Learning Algorithms: A Mini-Review

The accurate diagnosis of Alzheimer’s disease (AD) plays an important role in patient treatment, especially at the disease’s early stages, because risk awareness allows the patients to undergo preventive measures even before the occurrence of irreversible brain damage. Although many recent studies have used computers to diagnose AD, most machine detection methods are limited by congenital observations. AD can be diagnosed-but not predicted-at its early stages, as prediction is only applicable before the disease manifests itself. Deep Learning (DL) has become a common technique for the early diagnosis of AD. Here, we briefly review some of the important literature on AD and explore how DL can help researchers diagnose the disease at its early stages.
Circadian Rhythm of Blood Pressure Challenges Office Values as the “Gold Standard” in the Diagnosis of Gestational Hypertension

Despite poor sensitivity and specificity, office blood pressure (BP) determinations are still the “gold standard” for diagnosing gestational hypertension. This prospective blind study evaluates the prognostic value of office values as compared with ambulatory monitoring in pregnancy. We analyzed 2175 BP series systematically sampled from 355 non-preeclamptic pregnant women for 48 h every 4 wks from the first hospital visit until delivery. Women were divided for comparative purposes into three groups: “detected” gestational hypertension, defined on the basis of casual clinical BP>140/90 mm Hg after 20 wks of gestation and hyperbaric index (area of BP excess above the upper limit of a time-specified tolerance interval adjusted for the circadian pattern of the reference population) consistently above the threshold for diagnosing hypertension in pregnancy; “undetected” gestational hypertension, women with office BP<140/90 mm Hg but hyperbaric index consistently above the threshold for diagnosis; and normotension, women with both office values and hyperbaric index below the respective thresholds for diagnosis. Small and insignificant differences in the 24 h mean BP between “detected” and “undetected” gestational hypertension is observed in all trimesters, in contrast with highly significant differences between these two groups and normotensive pregnancies. Normotensive women are characterized by highly significant lesser incidence by 60% in preterm delivery, 70% in intrauterine growth retardation, and 50% in delivery by cesarean section (P<0.001) compared with women with “detected” and “undetected” gestational hypertension (P>0.715). In pregnancy, the hyperbaric index is markedly superior to office BP measurements for diagnosis of what should be truly considered gestational hypertension, and for prediction of the outcome of pregnancy.
How reliable is a RAID?

Disk arrays offer greatly increased transfer bandwidth at low cost, but without additional data redundancy they can suffer from significantly degraded reliability. The authors examine the reliability of RAID (redundant arrays of inexpensive disks) systems and find that although extremely reliable disk arrays cannot be attained with data redundancy alone, RAID system reliability can be made better than conventional large disks with little extra hardware. They show that the rest of the system components, such as the host bus adapters, power supplies, and fans cannot be ignored. The authors present a scheme in which the system support components are organized into groups orthogonal to the data redundancy groups, thus guaranteeing that no single disk or component failure will permanently lose data. This approach yields disk arrays with reliability about 50% greater than conventional disk subsystems. If further reliability improvements are sought, various parts of the support hardware can be made redundant. The authors have shown how these affect the design of a 49-data-disk RAID. <<ETX>>
A double-input flyback DC/DC converter with single primary winding

The traditional isolated multiple-input converter (MIC) has a simple configuration and can be used in the system with two or more sources. However, it has some disadvantages such as complex transformer structure due to multiple primary windings, higher voltage stresses on the power switches, and only one source transferring energy to the load at any time. This paper proposes a novel family of isolated MICs, in which the number of the transformer primary windings is reduced and the voltage stresses on the power switches are lower than that in the traditional multiple-input converter. A double-input Flyback DC/DC converter is taken as an example to analyze its operation principle in detail, and the voltage conversion ratio between the input and output is also deduced. Then the power management strategy for the proposed converter to distribute the power reasonably to the sources is proposed and it achieved the priority of the new energy utilization. A 120 W prototype with two input sources was built and tested, and the experimental results are presented to verify the theoretical analysis and the effectiveness of the control strategy.
High‐Resolution Melting Analysis (HRMA)—More than just sequence variant screening

Transition of the double‐stranded DNA molecule to its two single strands, DNA denaturation or melting, has been used for many years to study DNA structure and composition. Recent technological advances have improved the potential of this technology, especially to detect variants in the DNA sequence. Sensitivity and specificity were increased significantly by the development of so‐called saturating DNA dyes and by improvements in the instrumentation to measure the melting behavior (improved temperature precision combined with increased measurements per time unit and drop in temperature). Melt analysis using these new instruments has been designated high‐resolution melting curve analysis (HRM or HRMA). Based on its ease of use, simplicity, flexibility, low cost, nondestructive nature, superb sensitivity, and specificity, HRMA is quickly becoming the tool of choice to screen patients for pathogenic variants. Here we will briefly discuss the latest developments in HRMA and review in particular other applications that have thus far received less attention, including presequence screening, single nucleotide polymorphism (SNP) typing, methylation analysis, quantification (copy number variants and mosaicism), an alternative to gel‐electrophoresis and clone characterization. Together, these diverse applications make HRMA a multipurpose technology and a standard tool that should be present in any laboratory studying nucleic acids. Hum Mutat 30:1–7, 2009. © 2009 Wiley‐Liss, Inc.
Biography in late antiquity : a quest for the holy man

The best ebooks about Biography In Late Antiquity A Quest For The Holy Man that you can get for free here by download this Biography In Late Antiquity A Quest For The Holy Man and save to your desktop. This ebooks is under topic such as biography in late antiquity a quest for the holy man biography in late antiquity a quest for the holy man biography in late antiquity a quest for the holy man biography in late antiquity a quest for the holy man narrating the holy man in late antiquity: the case of holy bishops in late antiquity project muse constantine and the captive christians of persia bibliography the university of michigan press uncertain transformation: the role of asceticism in death introduction project muse beyond compare: pagan saint and christian god in late curriculum vitae 2012 name: patricia cox miller history of the donner party a tragedy sierra elosuk the face of the caesars: physiognomy in suetoniusÃ¢Â€ÂŸ de vita against capitalism pwcgba gedichte an sie marsal notes the university of michigan press theatre three thousand years of drama ac bilio frontier of control a study in british nrcgas st log seven stabuy department of corrections nj study guides mcsas hyundai robex 16 7 mini excavator workshop repair service air force manuals for gbrfu kumari mijn dochter uit nepal ffclub vitara manual reddye eieei an established journal of the history, structure turkey manicotti recipe viszon subtle bodies icon-painting school madden 08 strategy guide ps3 mifou justice league beyond 2
Pyrimidine‐specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus– biochemical characterization and homology modeling

We report the characterization of the pyrimidine‐specific ribonucleoside hydrolase from the hyperthermophilic archaeon Sulfolobus solfataricus (SsCU‐NH). The gene SSO0505 encoding SsCU‐NH was cloned and expressed in Escherichia coli and the recombinant protein was purified to homogeneity. SsCU‐NH is a homotetramer of 140 kDa that recognizes uridine and cytidine as substrates. SsCU‐NH shares 34% sequence identity with pyrimidine‐specific nucleoside hydrolase from E. coli YeiK. The alignment of the amino acid sequences of SsCU‐NH with nucleoside hydrolases whose 3D structures have been solved indicates that the amino acid residues involved in the calcium‐ and ribose‐binding sites are preserved. SsCU‐NH is highly thermophilic with an optimum temperature of 100 °C and is characterized by extreme thermodynamic stability (Tm = 106 °C) and kinetic stability (100% residual activity after 1 h incubation at 90 °C). Limited proteolysis indicated that the only proteolytic cleavage site is localized in the C‐terminal region and that the C‐terminal peptide is necessary for the integrity of the active site. The structure of the enzyme determined by homology modeling provides insight into the proteolytic analyses as well as into mechanisms of thermal stability. This is the first nucleoside hydrolase from Archaea.
Towards a Robust WiFi-based Fall Detection with Adversarial Data Augmentation

Recent WiFi-based fall detection systems have drawn much attention due to their advantages over other sensory systems. Various implementations have achieved impressive progress in performance, thanks to machine learning and deep learning techniques. However, many of such high accuracy systems have low reliability as they fail to achieve robustness in unseen environments. To address that, this paper investigates a method of generalization through adversarial data augmentation. Our results show a slight improvement in deep learning-systems in unseen domains, though the performance is not significant.
Patients’ moral views on coercion in mental healthcare

Background: Coercion in mental healthcare has led to ethical debate on its nature and use. However, few studies have explicitly explored patients’ moral evaluations of coercion. Aim: The purpose of this study is to increase understanding of patients’ moral views and considerations regarding coercion. Research design: Semi-structured focus-group and individual interviews were conducted and data were analysed through a thematic content analysis. Participants and research context: A total of 24 adult participants with various mental health problems and experiences with coercion were interviewed in 2012–2013 in three regions of Norway. Ethical considerations: Ethical approval and permissions were obtained according to required procedures. Informed consent and confidentiality were also secured. Findings: Ethical considerations regarding coercion included seven main themes: the need for alternative perspectives and solutions, the existence of a danger or harm to oneself or others, the problem of paternalism, the problem of discrimination and stigma, the need for proportionality, the importance of the content and consequences of coercion and concerns about way that coercion is carried out in practice. Discussion: The participants’ views and considerations are in line with previous research and reflect the range of normative arguments commonly encountered in ethical and legal debates. The study accentuates the significance of institutional factors and alternative voluntary treatment opportunities, as well as the legal and ethical principles of proportionality and purposefulness, in moral evaluations of coercion. Conclusion: Broader perspectives on coercion are required to comprehend its ethical challenges and derive possible solutions to these from a patient perspective.
Study of Multimodal Interfaces and the Improvements on Teleoperation

Research in multimodal interfaces aims to provide immersive solutions and to increase overall human performance. A promising direction is to combine auditory, visual and haptic interaction between the user and the simulated environment. However, no extensive comparison exists to show how combining audiovisuohaptic interfaces would affect human perception and by extent reflected on task performance. Our paper explores this idea and presents a thorough, full-factorial comparison of how all combinations of audio, visual and haptic interfaces affect performance during manipulation. We evaluated how each combination affects the performance in a study ( $N=25$ ) consisting of manipulation tasks with various difficulties. The overall performance was assessed using both subjective measures, by assessing cognitive workload and system usability, and objective measurements, by incorporating time and spatial accuracy-based metrics. The results showed that regardless of task complexity, the combination of stereoscopic-vision with the virtual reality headset increased performance across all measurements by 40%, compared to monocular-vision from a generic display monitor. Besides, using haptic feedback improved outcomes by 10% and auditory feedback accounted for approximately 5% improvement.
Rapid response to paroxetine is associated with plasma paroxetine levels at 4 but not 8 weeks of treatment, and is independent of serotonin transporter promoter polymorphism in Japanese depressed patients

In the present study, we investigated the relationship between the serotonin transporter gene linked polymorphic regions (5‐HTTLPR) or plasma paroxetine levels and clinical response to paroxetine in depressed patients. Sixty patients who met the DSM‐IV criteria for major depressive disorder were enrolled in the study. Twenty‐two were male and 38 were female, with ages ranging from 25 to 71 (mean ± SD = 42 ± 16) years. The clinical improvement of patients was assessed using the Hamilton rating scale for depression (Ham‐D) before and every week after paroxetine administration. According to the data reported previously, patients with an at least 50% decrease in their Ham‐D score were classified as responders. The results showed that the plasma paroxetine levels at 4 weeks were significantly higher in responders (rapid responders) than in nonresponders. On the other hand, no significant associations were found between the L genotype (L/L, L/S) or S genotype (S/S) and the response rates either at 4 weeks or 8 weeks. These results suggest that patients with higher plasma levels at 4 weeks might respond rapidly to paroxetine treatment, but the final response rate at 8 weeks will be independent of the plasma paroxetine levels and the 5‐HTTLPR L/S genotype. Copyright © 2009 John Wiley & Sons, Ltd.
Oxandrolone in the Treatment of HIV-Associated Weight Loss in Men: A Randomized, Double-Blind, Placebo-Controlled Study

Objective: To evaluate the efficacy and safety of oxandrolone in promoting body weight and body cell mass (BCM) gain in HIV-associated weight loss. Methods: Randomized, double-blind, placebo-controlled trial. Two hundred sixty-two HIV-infected men with documented 10% to 20% weight loss or body mass index ≤20 kg/m2 were randomized to placebo or to 20, 40, or 80 mg of oxandrolone daily. After 12 weeks, subjects were allowed to receive open-label oxandrolone at a dose of 20 mg for another 12 weeks. Results: Body weight increased in all groups, including the group receiving placebo, during the double-blind phase (1.1 ± 2.7, 1.8 ± 3.9, 2.8 ± 3.3, and 2.3 ± 2.9 kg in placebo and 20-, 40-, and 80-mg oxandrolone groups, respectively; all P < 0.014 vs. baseline). BCM increased from baseline in all groups (0.45 ± 1.7, 0.91 ± 2.2, 1.5 ± 2.5, and 1.8 ± 1.8 kg in placebo and 20-, 40-, and 80-mg oxandrolone groups, respectively). At 12 weeks, only the gain in weight at the 40-mg dose of oxandrolone and the gain in BCM at the 40- and 80-mg doses of oxandrolone were greater than those in the placebo group, however. Oxandrolone treatment was associated with significant suppression of sex hormone-binding globulin, luteinizing hormone, follicle-stimulating hormone, and total and free testosterone levels. Treatment was generally well tolerated but accompanied by significant increases in transaminases and low-density lipoprotein as well as decreases in high-density lipoprotein. Conclusion: Oxandrolone administration is effective in promoting dose-dependent gains in body weight and BCM in HIV-infected men with weight loss.
Investigations on Ultrawideband Pentagon Shape Microstrip Slot Antenna for Wireless Communications

An ultrawideband (UWB) pentagon shape planar microstrip slot antenna is presented that can find applications in wireless communications. Combination of the pentagon shape slot, feed line and pentagon stub are used to obtain 124% (2.65-11.30 GHz) impedance bandwidth which exceeds the UWB requirement of 110% (3.10-10.60 GHz). A ground plane of 50 mm &times; 80 mm size is used which is similar to wireless cards for several portable wireless communication devices. The proposed antenna covers only the top 20 mm or 25% of the ground plane length, which leaves enough space for the RF circuitry. Three variations of the antenna design using the straight and rotated feed lines on two different substrates are considered. Effect of the conducting reflecting sheet on back of the antenna is investigated, which can provide directional radiation patterns but with reduced matching criteria. Finally, experimental verification of the fabricated antenna for its impedance bandwidth is carried out, which shows agreement with the simulated data.