Miracle-dz/UniFAR-FTUs huggingface dataset:
Transport Network Orchestration for End-to-End Multilayer Provisioning Across Heterogeneous SDN/OpenFlow and GMPLS/PCE Control Domains

A multidomain optical transport network composed of heterogeneous optical transport technologies (e.g., flexi/fixed-grid optical circuit switching and optical packet switching) and control plane technologies (e.g., centralized OpenFlow or distributed GMPLS) does not naturally interoperate, and a network orchestration mechanism is required. A network orchestrator allows the composition of end-to-end network service provisioning across multidomain optical networks comprising different transport and control plane technologies. Software-defined networking (SDN) is a key technology to address this requirement, since the separation of control and data planes makes the SDN a suitable candidate for end-to-end provisioning service orchestration across multiple domains with heterogeneous control and transport technologies. This paper presents two different network orchestration's architectures based on the application-based network operations (ABNO) which is being defined by IETF based on standard building blocks. Then, we experimentally assesses in the international testbed of the STRAUSS project, an ABNO-based network orchestrator for end-to-end multi-layer (OPS and Flexi-grid OCS) and multidomain provisioning across heterogeneous control domains (SDN/OpenFlow and GMPLS/Stateful PCE) employing dynamic domain abstraction based on virtual node aggregation.
Phosphorylation within the amino-terminal acidic domain I of the phosphoprotein of vesicular stomatitis virus is required for transcription but not for replication

Phosphorylation by casein kinase II at three specific residues (S-60, T-62, and S-64) within the acidic domain I of the P protein of Indiana serotype vesicular stomatitis virus has been shown to be critical for in vitro transcription activity of the viral RNA polymerase (P-L) complex. To examine the role of phosphorylation of P protein in transcription as well as replication in vivo, we used a panel of mutant P proteins in which the phosphate acceptor sites in domain I were substituted with alanines or other amino acids. Analyses of the alanine-substituted mutant P proteins for the ability to support defective interfering RNA replication in vivo suggest that phosphorylation of these residues does not play a significant role in the replicative function of the P protein since these mutant P proteins supported replication at levels > or = 70% of the wild-type P-protein level. However, the transcription function of most of the mutant proteins in vivo was severely impaired (2 to 10% of the wild-type P-protein level). The level of transcription supported by the mutant P protein (P(60/62/64)) in which all phosphate acceptor sites have been mutated to alanines was at best 2 to 3% of that of the wild-type P protein. Increasing the amount of P(60/62/64) expression in transfected cells did not rescue significant levels of transcription. Substitution with other amino acids at these sites had various effects on replication and transcription. While substitution with threonine residues (P(TTT)) had no apparent effect on transcription (113% of the wild-type level) or replication (81% of the wild-type level), substitution with phenylalanine (P(FFF)) rendered the protein much less active in transcription (< 5%). Substitution with arginine residues led to significantly reduced activity in replication (6%), whereas glutamic acid substituted P protein (P(EEE)) supported replication (42%) and transcription (86%) well. In addition, the mutant P proteins that were defective in replication (P(RRR)) or transcription (P(60/62/64)) did not behave as transdominant repressors of replication or transcription when coexpressed with wild-type P protein. From these results, we conclude that phosphorylation of domain I residues plays a major role in in vivo transcription activity of the P protein, whereas in vivo replicative function of the protein does not require phosphorylation. These findings support the contention that different phosphorylated states of the P protein regulate the transcriptase and replicase functions of the polymerase protein, L.
Draft Genome Sequence of Geotrichum candidum Strain 3C

ABSTRACT We report here the draft genome sequence of Geotrichum candidum strain 3C, which is a filamentous yeast-like fungus that holds great promise for biotechnology. The genome was sequenced using Ion Torrent and 454 platforms. The estimated genome size was 41.4 Mb, and 14,579 protein-coding genes were predicted ab initio.
Building a predictable avionics platform in Ada: a case study

In an effort to promote the use of Ada in embedded system development, a realistic avionics application model whose hard real-time properties are fully predictable has been generated. This was done starting with a rate monotonic analysis of a set of typical fully synchronized periodic and aperiodic avionics tasks drawn from an idealized attack aircraft mission computer. The authors describe this avionics case study and its goals, examine the resulting design issues and trade-offs, and investigate the ability of the rate monotonic analysis model to predict its performance under a heavy load. <<ETX>>
Telomerase and mTOR in the brain: the mitochondria connection

Telomerase is an enzyme that maintains telomeres in dividing cells using a template on its inherent RNA component. Additionally, the protein part TERT (Telomerase Reverse Transcriptase) has various non-canonical functions. For example, it can localize to mitochondria under increased stress and protect cells in vitro from oxidative stress, DNA damage and apoptosis. Recently it has been demonstrated that TERT protein persists in adult neurons in the brain and data emerge suggesting that it might have a protective function in these post-mitotic cells as well. We have recently published that TERT protein accumulated in mitochondria from brain tissue of mice that have undergone short-term dietary restriction (DR) and rapamycin treatment. This localization correlated to lower levels of oxidative stress in these brain mitochondria. Since rapamycin treatment decreases mTOR signaling which is also thought to play an important role for the beneficial effects of DR, we conclude that the mTOR pathway might be involved in the TERT localization and its effects in brain mitochondria in vivo. These data are in line with previous findings from our group about increased mitochondrial localization of TERT in Alzheimer's disease (AD) brains and a protective function of TERT protein in neurons in vitro against pathological tau.
DEC1 Modulates the Circadian Phase of Clock Gene Expression

ABSTRACT DEC1 suppresses CLOCK/BMAL1-enhanced promoter activity, but its role in the circadian system of mammals remains unclear. Here we examined the effect of Dec1 overexpression or deficiency on circadian gene expression triggered with 50% serum. Overexpression of Dec1 delayed the phase of clock genes such as Dec1, Dec2, Per1, and Dbp that contain E boxes in their regulatory regions, whereas it had little effect on the circadian phase of Per2 and Cry1 carrying CACGTT E′ boxes. In contrast, Dec1 deficiency advanced the phase of the E-box-containing clock genes but not that of the E′-box-containing clock genes. Accordingly, DEC1 showed strong binding and transrepression on the E box, but not on the E′ box, in chromatin immunoprecipitation, electrophoretic mobility shift, and luciferase reporter assays. Dec1−/− mice showed behavioral rhythms with slightly but significantly longer circadian periods under conditions of constant darkness and faster reentrainment to a 6-h phase-advanced shift of a light-dark cycle. Knockdown of Dec2 with small interfering RNA advanced the phase of Dec1 and Dbp expression, and double knockdown of Dec1 and Dec2 had much stronger effects on the expression of the E-box-containing clock genes. These findings suggest that DEC1, along with DEC2, plays a role in the finer regulation and robustness of the molecular clock.
Data analysis techniques in phosphoproteomics

The interpretation of phosphoproteomics data sets is crucial for generating hypotheses that guide therapeutic solutions, yet not many techniques have been applied to this type of analysis. This paper intends to give an overview about the two main standard techniques that can be applied to the analysis of these large scale data sets. These are data‐driven or exploratory techniques based on a statistical model and topology‐driven methods that analyze the signaling network from a dynamical standpoint. While employing different paradigms, these algorithms will detect unique “fingerprints” by revealing the intricate interactions at the proteome level and will support the experimental environment for novel therapeutics for many diseases.
KSHV vFLIP binds to IKK-γ to activate IKK

When expressed in heterologous cells, the viral FLIP protein (vFLIP) of Kaposi's-sarcoma-associated herpesvirus (KSHV) has been reported both to block Fas-mediated apoptosis and to activate the NF-κB activation pathway by interaction with IκB kinase (IKK). In a yeast-two-hybrid screen, we identified IKKγ as an interacting partner of vFLIP. We expressed fragments of IKKγ in mammalian cells and bacteria, and identified the central CCR3/4 (amino acids 150-272) as the vFLIP binding region. To investigate the proteins interacting with vFLIP in a KSHV-infected primary effusion lymphoma (PEL) cell line, we immunoprecipitated vFLIP and identified four associated proteins by mass spectrometry: IKK components IKKα, β and γ, and the chaperone, Hsp90. Using gel filtration chromatography, we demonstrated that a single population of vFLIP in the cytoplasm of PEL cells co-eluted and co-precipitated with an activated IKK complex. An inhibitor of Hsp90, geldanamycin, inhibited IKK's kinase activity induced by vFLIP and killed PEL cells, suggesting that vFLIP activation of IKK contributes to PEL cell survival.
Mathematical Modelling of Identity, Identity Management and Other Related Topics

There exist disparate sets of definitions with different semantics on different topics of Identity Management which often lead to misunderstanding. A few efforts can be found compiling several related vocabularies into a single place to build up a set of definitions based on a common semantic. However, these efforts are not comprehensive and are only textual in nature. In essence, a mathematical model of identity and identity management covering all its aspects is still missing. In this paper we build up a mathematical model of different core topics covering a wide range of vocabularies related to Identity Management. At first we build up a mathematical model of Digital Identity. Then we use the model to analyse different aspects of Identity Management. Finally, we discuss three applications to illustrate the applicability of our approach. Being based on mathematical foundations, the approach can be used to build up a solid understanding on different topics of Identity Management.
Seismic Imaging of Microblocks and Weak Zones in the Crust Beneath the Southeastern Margin of the Tibetan Plateau

The studies of Earth's history and of the physical and chemical properties of the substances that make up our planet, are of great significance to our understanding both of its past and its future. The geological and other environmental processes on Earth and the composition of the planet are of vital importance in locating and harnessing its resources. This book is primarily written for research scholars, geologists, civil engineers, mining engineers, and environmentalists. Hopefully the text will be used by students, and it will continue to be of value to them throughout their subsequent professional and research careers. This does not mean to infer that the book was written solely or mainly with the student in mind. Indeed from the point of view of the researcher in Earth and Environmental Science it could be argued that this text contains more detail than he will require in his initial studies or research.
Study and Analysis of Clustering Based Scalable Recommender Systems for E-Commerce

Collaborative filtering based recommender systems help online users in choosing the right products based on the purchase history of the user and his most similar users. Scalability is one of the major issues in designing effective recommender system. In this paper, we have studied different ways of increasing scalability by applying clustering algorithms on three types collaborative filtering algorithms-user based, item based and slope one. Finally we have analyzed the relationship between scalability and accuracy for different number of clusters and neighborhoods.
Step-by-step measurements of soft tissue balancing during total knee arthroplasty for patients with varus knees.

Using a step-by-step procedure, we measured joint gap during surgery using a simple device with a torque meter in 45 osteoarthritis knee joints (43 patients) with varus deformity. The effects of specific cuts or releases of the anatomic portion on joint gaps were investigated. Each cut or release resulted in various increases in the medial gap from 1.2 to 3.8 mm on average. The final gap measurements averaged 24.1 mm medially and 27.6 mm laterally in extension, and 24.6 mm medially and 27.2 mm laterally in flexion. The results of the measurements showed that each step-by-step procedure had a tendency of gap increase. The results led us to measure soft tissue balancing in a step-by-step procedure during total knee arthroplasty.
Thrombosis and Aspirin: Clinical Aspect, Aspirin in Cardiology, Aspirin in Neurology, and Pharmacology of Aspirin

Acetyl salicylic acid or aspirin is one of the most famous drugs in the world. Aspirin has been increasingly used for prevention of cardiovascular events and is, particularly in recent decades, the most used nonsteroidal anti-inflammatory drug. Thus, it was necessary to have several contributions to precise the interest and the side effects of aspirin in cardiology, pharmacology, and neurology. A general review on aspirin pharmacology was made by Espinosa et al. (Western University of Health Sciences, Pomona, CA, USA). This review article helps to understand the role of platelets in primary hemostasis and atherothrombosis, the use of aspirin in the prevention, and treatment of cardiovascular diseases. In the review article by B. Rocca and G. Petrucci (Catholic University School of Medicine, Rome, Italy), they have detailed the phenomenon of variability in the responsiveness to low-dose aspirin and reported the explanations of this real problem in patients. The clinical use of aspirin in treatment and prevention of cardiovascular diseases was the subject of the work of Y. Dai and J. Ge (Fudan University, Shanghai, China). In this paper, the authors review the different clinical situations when aspirin is administered. The use of aspirin for prevention of thrombosis was clearly discussed by G. H. R. Rao and J. Fareed (University of Minnesota, Minneapolis, MN and Loyola University Medical Center, Maywood, IL, USA). The special work on the role of dermcidin isoform 2 by Ghosh et al. (Calcutta, India) completes the two clinical papers. The work of Losche et al. (Jena University Hospital, Jena, Germany) on the problem of the reduction of mortality in critically ill patients also demonstrates the effectiveness of antiplatelet drugs to prevent organ failure. The three experimental studies from the laboratory of Doutremepuich concern the paradoxical action of aspirin used at different dosages. These different effects can explain the thrombosis observed in clinical practice after aspirin discontinuation. The question could be also: what is the effect of a drug at ultralow dose? In conclusion, this special issue can represent a “state of art” on aspirin and thrombosis with new hypotheses of work.
Research of Aircraft Integrated Drive Generator fault diagnostic decision based on attribute reduction in rough sets

For purpose of solve the problems of failure of multi-source and complex diagnostic decision in the process of Aircraft Integrated Drive Generator fault diagnosing, from the perspective of data mining, reference the variable precision rough sets to dispose the diagnostic information system, deduce judgment theorems for judging β lower approximation consistent sets by the binary relation and dependence space which defined by a set of objects, put forward the Aircraft Integrated Drive Generator fault diagnostic decision based on β lower approximation attribute reduction algorithm, which by decision system obtained from real generator output fault signal, to demonstrate its effectiveness and universality in case of small samples.
Prevalence of Asthma and Allergic Rhinitis in Children with Celiac Disease

Background: Celiac disease is an autoimmune disorder characterized by damage to the small intestinal mucosa following the intake of glutencontaining foods in genetically predisposed individuals. Studies suggest that celiac disease is associated with asthma and allergic rhinitis. Objectives: The aim of this study was to examine the prevalence of asthma and allergic rhinitis in children with celiac disease compared to healthy controls. Methods: This study was conducted in 53 celiac children and 80 children with nonspecific abdominal pain as a control group; all participants were between 6 19 years. The children completed the ISAAC questionnaire, and spirometry was performed in each participant. Results: The prevalence rates of asthma symptoms and physician-diagnosed asthma were similar in both groups (30% and 19%, respectively, in the celiac group and 17.5% and 22.5%, respectively, in the control group) (P > 0.05). Six (11%) patients with celiac disease and seven (9%) children in the control group showed obstructive changes on pulmonary function tests. The prevalence rates of allergic rhinitis symptoms and physician-diagnosed allergic rhinitis were also similar in both groups (36% and 9%, respectively, in the celiac group, and 34% and 9%, respectively, in the control group) (P > 0.05). Conclusions: The prevalence rates of asthma and allergic rhinitis in patients with celiac disease were not significantly higher when compared to the healthy controls.
Modified Subspace Based Channel Estimation Algorithm for OFDM Systems

A modified subspace-based blind channel estimation algorithm for OFDM systems is proposed in this paper. A systematic approach is used to construct the signal matrix in the proposed algorithm. Compared with conventional subspacebased blind channel estimation algorithm, the proposed algorithm has lower computational complexity and higher probability of full row rank for the corresponding signal matrix. In addition, fewer OFDM symbols can be used to satisfy the necessary condition for achieving a full-row-rank signal matrix. Simulation results show that the proposed algorithm outperforms conventional method in mean-squared error and bit error rate under static channel. Even with a smaller number of received OFDM symbols, the proposed algorithm can perform well.
Evaluation of Counting Efficiency of Whole – body Counter using Voxel Phantoms

The calibration methods for in vivo measurement systems using Monte Carlo simulations and voxel phantoms have been demonstrated. The calibration data, such as counting efficiency or response function, calculated by the UCWBC code that was developed in the Japan Atomic Energy Research Institute were compared with those measured with an in vivo measurement system. It was found that the calibration data calculated by the UCWBC code show good agreement with measured ones. The mathematical calibration method of in vivo measurement systems using voxel phantoms was found to be useful for the improvement in accuracy of the measurement results.
Large Scale Anthropogenic Reduction of Forest Cover in Last Glacial Maximum Europe

Reconstructions of the vegetation of Europe during the Last Glacial Maximum (LGM) are an enigma. Pollen-based analyses have suggested that Europe was largely covered by steppe and tundra, and forests persisted only in small refugia. Climate-vegetation model simulations on the other hand have consistently suggested that broad areas of Europe would have been suitable for forest, even in the depths of the last glaciation. Here we reconcile models with data by demonstrating that the highly mobile groups of hunter-gatherers that inhabited Europe at the LGM could have substantially reduced forest cover through the ignition of wildfires. Similar to hunter-gatherers of the more recent past, Upper Paleolithic humans were masters of the use of fire, and preferred inhabiting semi-open landscapes to facilitate foraging, hunting and travel. Incorporating human agency into a dynamic vegetation-fire model and simulating forest cover shows that even small increases in wildfire frequency over natural background levels resulted in large changes in the forested area of Europe, in part because trees were already stressed by low atmospheric CO2 concentrations and the cold, dry, and highly variable climate. Our results suggest that the impact of humans on the glacial landscape of Europe may be one of the earliest large-scale anthropogenic modifications of the earth system.
Quality of life assessment in patients undergoing nucleoplasty-based percutaneous discectomy.

OBJECT Nucleoplasty is a minimally invasive surgical procedure for disc decompression developed to treat patients with symptomatic contained herniated discs. Nucleoplasty uses nonheat-driven radiofrequency energy to ablate and coagulate the disc nucleus via a percutaneous "discography" trajectory under fluoroscopic guidance. In this study the authors evaluated pain, functioning, and quality of life (QOL) in patients with radicular leg and back pain who underwent nucleoplasty-based percutaneous disc decompression. METHODS The study was designed as a prospective nonrandomized longitudinal cohort study in an academic medical center. Sixty-seven patients (mean age 41 years) with primarily radicular pain due to a contained disc herniation underwent nucleoplasty-based decompression in an outpatient setting. Patients completed the Medical Outcomes Study 36-Item Short Form (SF-36) Health Survey, EuroQol 5D (EQ5D), and a visual analog scale (VAS) for pain preoperatively, and at 3 and 6 months after surgery. Postoperative QOL differences were assessed using the Wilcoxon signed-rank test. A surgical probe, the Perc-DLE SpineWand, was placed percutaneously into the disc after application of a local anesthetic or induction of general anesthesia to remove part of the disc (that is, a percutaneous discectomy). Nucleoplasty-treated levels were L2-3 (one case), L3-4 (five cases), L4-5 (44 cases), and L5-S 1 (40 cases); there were 22 multiple treatment levels and 42 bilateral treatments. There were no infections or nerve root injuries associated with the procedure. Compared with preoperative QOL, there was a statistically significant improvement in QOL at 3 months as measured using the SF-36 Physical Component Summary (PCS) scale (mean score improvement 4.4 [p = 0.014]), the EQ5D (mean score improvement 0.22 [p = 0.001]), and the VAS for pain (mean score improvement 0.13 [p = 0.021). Six-month results in 36 patients continued to reflect improvement as measured using the SF-36 PCS (mean score improvement 7.6 [p = 0.002]) and the EQ5D (mean score improvement 0.27 [p = 0.001]). CONCLUSIONS Nucleoplasty-based percutaneous disc decompression in patients with symptomatic contained disc herniations is safe and improves QOL as measured by the SF-36, EQ5D, and VAS for pain, three generic QOL outcome instruments. Nucleoplasty is an effective minimally invasive surgical treatment alternative in patients with symptomatic contained disc hemiations. Further follow-up evaluation is underway to determine the durability of QOL improvement after nucleoplasty.