Miracle-dz/UniFAR-FTUs huggingface dataset:
Increased oxygen cost of contractility in the endotoxemic porcine left ventricle

Objective—Myocardial oxygen consumption (MVO 2 ) in the septic myocardium is comparatively high in relation to the sepsis‐induced reduction in ventricular work. Our previous studies indicate that this energetic inefficiency is due to increased energy consumption in excitation–contraction (EC) coupling, i.e. myocardial calcium handling. Design—To further confirm this observation, we assessed the oxygen cost of contractility in anesthetized pigs before and 2 h after induction of endotoxemia (1 μg/kg endotoxin infusion over 1 h, Escherichia coli toxin, n=6). Baroreceptor reflexes were blocked by hexamethonium. Contractility was increased by stepwise dopamine infusions at baseline and 2 h after induction of endotoxemia. Oxygen cost of contractility was assessed as the relationship between myocardial contractility (E es or elastance) and non‐mechanical oxygen consumption (unloaded MVO 2 ), a measure of energy consumption in EC coupling or calcium handling. Results—Non‐mechanical oxygen consumption (unloaded MVO 2 ) was higher after endotoxin infusions than at baseline (0.641±0.05 vs 0.383±0.07 J/beat/100 g, p < 0.05). The relationship between unloaded MVO 2 and E es , constructed by the dopamine response, was highly linear both at baseline and endotoxemia (r 2 =0.76–0.99). However, endotoxin increased oxygen cost of contractility by ∼45% (baseline 0.06±0.03 vs endotoxin 0.09±0.04 J ml/mmHg/beat/100 g). Conclusion—Acute endotoxemia increases oxygen cost of contractility, a measure of energy consumed in EC coupling or myocardial calcium handling.
Dietary fat attenuates the benefits of an elemental diet in active Crohn's disease: a randomized, controlled trial

Objectives Although an elemental diet has been established as the primary treatment for patients with Crohn's disease, the influence of dietary fat on the elemental diet remains unclear. We have designed the first randomized, controlled trial for elemental diets containing different fat percentages in patients with active Crohn's disease. Methods Each patient was randomized to receive one of three dose levels of fat in an elemental diet (Elental) for 4 weeks: 10 patients received low fat (3.06 g/day), 10 patients received medium fat (16.56 g/day) and eight patients received high fat (30.06 g/day). The additional fat was composed of long-chain fatty acids. All patients were evaluated using the International Organization of Inflammatory Bowel Disease rating, plus C-reactive protein level and erythrocyte sedimentation rate, which were measured at weekly intervals. Results Although the International Organization of Inflammatory Bowel Disease rating, C-reactive protein level and erythrocyte sedimentation rate in the low-fat group decreased, the values in the medium- and high-fat groups fluctuated during the study. The remission rate after 4 weeks in each group was 80%, 40% and 25% for patients in the low-, medium- and high-fat groups, respectively. Conclusions When the fat consisted of long-chain triglycerides, a high amount of this fat in the elemental diet formula decreased its therapeutic effect against active Crohn's disease.
Inhalation Tolerance Is Induced Selectively in Thoracic Lymph Nodes but Executed Pervasively at Distant Mucosal and Nonmucosal Tissues1

Under immunogenic conditions, both the site of initial Ag exposure and consequent T cell priming in specific draining lymph nodes (LNs) imprint the ensuing immune response with lasting tissue-selective tropism. With respect to immune tolerance, whether the site of tolerance induction leads to compartmentalized or, alternatively, pervasive tolerance has not been formally investigated. Using a murine model of inhalation tolerance, we investigated whether the induction of respiratory mucosal tolerance precludes the development of de novo Th2 sensitization upon subsequent exposure to the same Ag at distant mucosal (gut) and nonmucosal (cutaneous) sites. By tracking the proliferation of CFSE-labeled OVA-TCR transgenic CD4+ T cells upon OVA inhalation in vivo, we defined the site of tolerance induction to be restricted to the thoracic LNs. Expectedly, inhalation tolerance prevented de novo Th2 sensitization upon subsequent exposure to the same Ag at the same site. Importantly, although gut- and skin-draining LNs were not used during tolerance induction, de novo Ag-specific proliferation and Th2 differentiation in these LNs, as well as memory/effector Th2 responses in the gut (allergic diarrhea) and skin (late-phase cutaneous responses) were inhibited upon immunogenic challenge to the same Ag. Interestingly, this pervasive tolerogenic phenotype was not associated with the presence of suppressive activity throughout the lymphatics; indeed, potent suppressive activity was detected solely in the spleen. These data indicate that while inhalation tolerance is selectively induced in local thoracic LNs, its tolerogenic activity resides systemically and leads to pervasive immune tolerance in distant mucosal and nonmucosal sites.
Modeling and analysis of cellular networks with elastic data traffic

We devise a framework using tools from stochastic geometry and queuing theory for the study of irregular cellular networks when user traffic varies randomly in time and space. We consider a typical wireless cell with a guard zone surrounded by an interference environment comprised of a dominant node at the guard-edge plus an outer-bound Poisson field of sources. A systematic approach is presented to characterize the flow rate in the presence of elastic data traffic with closed-form expressions of the intended signal power and bounded aggregate interference statistics over Nakagami-m fading channels accordingly derived. We then formulate and solve an optimization problem for the computation of the traffic capacity defined as the maximum elastic data flow intensity for which the system remains unsaturated.
Physical and Performance Characteristics Related to Unintentional Musculoskeletal Injury in Special Forces Operators: A Prospective Analysis.

CONTEXT Seventy-seven percent of musculoskeletal injuries sustained by United States Army Special Forces Operators are preventable. Identification of predictive characteristics will promote the development of screening methods to augment injury-prevention programs. OBJECTIVE To determine physical and performance characteristics that predict musculoskeletal injuries. SETTING Clinical laboratory. PATIENTS OR OTHER PARTICIPANTS A total of 95 Operators (age = 32.7 ± 5.1 years, height = 179.8 ± 6.9 cm, mass = 89.9 ± 12.7 kg). MAIN OUTCOME MEASURE(S) Laboratory testing consisted of body composition, aerobic and anaerobic capacity, upper and lower body strength and flexibility, balance, and biomechanical evaluation. Injury data were captured for 12 months after laboratory testing. Injury frequencies, cross-tabulations, and relative risks (RRs) were calculated to evaluate the relationships between physical characteristics and injury proportions. Between-groups differences (injured versus uninjured) were assessed using appropriate t tests or Mann-Whitney U tests. RESULTS Less shoulder-retraction strength (RR = 1.741 [95% confidence interval = 1.003, 3.021]), knee-extension strength (RR = 2.029 [95% confidence interval = 1.011, 4.075]), and a smaller trunk extension : flexion ratio (RR = 0.533 [95% confidence interval = 0.341, 0.831]) were significant risk factors for injury. Group comparisons showed less trunk strength (extension: P = .036, flexion: P = .048) and smaller right vertical ground reaction forces during landing ( P = .025) in injured Operators. Knee strength, aerobic capacity, and body mass index were less in the subgroup of spine-injured versus uninjured Operators ( P values = .013-.036). CONCLUSIONS Knee-extension and shoulder-retraction strength were risk factors for musculoskeletal injury in Operators. Less trunk-flexion and -extension strength, higher body mass index, lower aerobic capacity, and increased ground reaction forces during landing were characteristics that may also contribute to musculoskeletal injury. Having 2 or more risk factors resulted in a greater injury proportion (χ2 = 13.512, P = .015); however, more research is needed. Athletic trainers working in the military or similar high-demand settings can use these data to augment screening and injury-prevention protocols.
Design and implementation of a drawbot using Matlab and Arduino Mega

Recent research on robots has been trying to develop intelligent robots that can match human behavior on high level intelligent tasks that require sensing, complex motion and intelligence. In the recent few years robot is given some artistic behavior that robot can sing, dance even robot can play games. This paper presents a control method for a robotic arm to let the robot to acquire another human artistic behavior “drawing”. In the proposed design MATLAB has been used for image processing interface and trajectory calculation. Value of co-ordinate position of a pixel of binary image is converted into joint angle applying inverse kinematics and servos are controlled with Arduino Mega. Sobel and Canny are applied for edge detection method and authors showed comparison between the output images after applying Sobel and Canny edge detection method. Image is given input through Matlab GUI, which is user interactive.
High-energy tail electrons as the mechanism for the worst-case hot-carrier stress degradation of the deep submicrometer N-MOSFET

Experimental evidence, based on sensitively modulating the concentration of the high-energy tail of the electron energy distribution, reveals an important trend in the mid-to-high gate stress voltage (V/sub g/) regime, where device degradation is seen to continuously increase with the applied V/sub g/, for a given drain stress voltage V/sub d/. The shift in the worst-case degradation point from V/sub g//spl ap/V/sub d//2 to V/sub g/=V/sub d/, depicting an uncorrelated behavior with the substrate current, is caused by the injection of the high-energy tail electrons into the gate oxide, when the oxide field near the drain region becomes increasingly favorable as V/sub g/ approaches V/sub d/. This letter offers an improved framework for understanding the worst-case hot-carrier stress degradation of deep submicrometer N-MOSFETs under low bias condition.
Particle flow characterization for a microfluidic device for cell-based assay

Fluid velocity fields and particle motions were experimentally studied for the application of microfluidics in chip-based cell assays. muPIV measurements were conducted on diverging microchannels and a microfluidic cell trapping device to characterize the respective fluid flow fields. A highspeed camera was then used to capture and characterize the motion of particles released into the corresponding flows. The distributions of the particle pathlines when undergoing divergence were shown to have negligible inertial effects. Whereas deviations of pathlines within the cell trapping device and were attributed to the design of the macro-micro interfaces in the packaging.
Gender differences in risk of renal cell carcinoma and occupational exposures to chlorinated aliphatic hydrocarbons.

BACKGROUND Organic solvents have been associated with renal cell cancer; however, the risk by gender and type of solvents is nuclear. METHODS We evaluated the risk of renal cell carcinoma among men and women exposed to all organic solvents-combined, all chlorinated aliphatic hydrocarbons (CAHC)-combined, and nine individual CAHC using a priori job exposure matrices developed by NCI in a population-based case-control study in Minnesota, U.S. We interviewed 438 renal cell cancer cases (273 men and 165 women) and 687 controls (462 men and 225 women). RESULTS Overall, 34% of male cases and 21% of female cases were exposed to organic solvents in general. The risk of renal cell carcinoma was significantly elevated among women exposed to all organic solvents combined (OR = 2.3; 95% CI = 1.3-4.2), to CAHC combined (OR = 2.1; 95% CI = 1.1-3.9), and to trichloroethylene (TCE) (OR = 2.0; 95% CI = 1.0-4.0). Among men, no significant excess risk was observed among men exposed to any of these nine individual CAHCs, all CAHCs-combined, or all organic solvents-combined. DISCUSSION These observed gender differences in risk of renal cell carcinoma in relation to exposure to organic solvents may be explained by chance based on small numbers, or by the differences in body fat content, metabolic activity, the rate of elimination of xenobiotics from the body, or by differences in the level of exposure between men and women, even though they have the same job title.
Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis

Multicellular organs are composed of distinct cell types with unique assemblages of translated mRNAs. Here, ribosome-associated mRNAs were immunopurified from specific cell populations of intact seedlings using Arabidopsis thaliana lines expressing a FLAG-epitope tagged ribosomal protein L18 (FLAG-RPL18) via developmentally regulated promoters. The profiling of mRNAs in ribosome complexes, referred to as the translatome, identified differentially expressed mRNAs in 21 cell populations defined by cell-specific expression of FLAG-RPL18. Phloem companion cells of the root and shoot had the most distinctive translatomes. When seedlings were exposed to a brief period of hypoxia, a pronounced reprioritization of mRNA enrichment in the cell-specific translatomes occurred, including a ubiquitous rise in 49 mRNAs encoding transcription factors, signaling proteins, anaerobic metabolism enzymes, and uncharacterized proteins. Translatome profiling also exposed an intricate molecular signature of transcription factor (TF) family member mRNAs that was markedly reconfigured by hypoxia at global and cell-specific levels. In addition to the demonstration of the complexity and plasticity of cell-specific populations of ribosome-associated mRNAs, this study provides an in silico dataset for recognition of differentially expressed genes at the cell-, region-, and organ-specific levels.
The influence of nonlinear fields on miniature hydrophone calibration using the planar scanning technique

Miniature polyvinylidene fluoride (PVDF) hydrophones used in determining the power and intensity output of ultrasonic fields, including those radiated from diagnostic ultrasound equipment, were calibrated under a variety of field conditions using the planar scanning technique. A diagnostic B-scan piston-type transducer was used as a source, and the output intensity (spatial-peak pulse-average, or SPPA) was varied from 2 to 30 W/cm/sup 2/ while the total power was kept constant. The higher-intensity waveforms were significantly nonlinear in the focal region of the source. When the lateral beam profiles of the source (as measured by the hydrophone to be calibrated) were determined by positive-peak-detecting the ultrasonic pulse, the calibrated pressure sensitivity of the hydrophone systematically decreased as the field became progressively more nonlinear. When the beam profiles were measured using the pulse intensity integral, no systematic dependence of the calibration on field linearity was noted. These results imply that measured values of power and intensity of ultrasound diagnostic equipment may be dependent on the methodology utilized to map the lateral beam profiles of the transducer being measured, and the extent of nonlinear effects in the field under characterization. <<ETX>>
Cryogenically Cooled Millimetre-Wave Front Ends for the Australia Telescope

Cryogenically cooled receiver systems covering three millimetre-wave observing bands have been installed on the Australia Telescope National Facility Compact Array (ATCA), situated near Narrabri in northern New South Wales, Australia. The ATCA consists of six 22-metre dish antennas capable of operating at frequencies in excess of 115 GHz. Millimetre-wave receiver systems covering the frequency ranges 16 to 26 GHz and 85 to 105 GHz have been upgraded with the installation of a receiver system covering the 30 to 50 GHz band. We will describe the final receiver system and present the results of tests on these receiver systems.
Gonadotropin-releasing hormone pulses in third ventricular cerebrospinal fluid of ovariectomized rhesus monkeys: correlation with luteinizing hormone pulses.

Morphological evidence suggests that GnRH may be released into cerebrospinal fluid (CSF) of the third ventricle. Therefore, a method of cannulating the third ventricle of monkey brains was developed for the purpose of examining GnRH secretion in primates. A stainless steel guide cannula was stereotaxically implanted into the third ventricle of 14 ovariectomized rhesus monkeys. A Silastic cannula for collecting CSF was inserted via the guide cannula into the ventral portion of the ventricle, permitting repeated CSF sampling for long time periods from the same animal. One week to 6 months after cannulation, CSF was collected continuously for periods of 5-10 h at 2 different rates (480 and 120 microliter/h) from conscious monkeys seated in chairs. Samples were divided into 15-min fractions, and the GnRH concentration in each was determined by RIA. In contrast to most previous studies, third ventricular CSF was found to contain significant concentrations of GnRH. GnRH was detected in 40 of 50 collections. Concentrations ranged from less than 8 to greater than 800 pg/ml, a range similar to that observed in hypophyseal portal blood. Furthermore, fluctuations within individual collections indicated that GnRH was released in pulses. The mean GnRH pulse frequency during the higher rate of CSF withdrawal was 0.43 +/- 0.06 pulses/h (n = 31), while the mean pulse amplitude was 91 +/- 7 pg/ml (n = 64). Neither parameter was influenced by the rate of CSF removal, as frequency was 0.52 +/- 0.08 pulses/h (n = 19) and amplitude was 94 +/- 11 pg/ml (n = 82) during the lower collection rate. However, the CSF withdrawal rate had a profound influence on LH secretion. In 12 of 17 collections at the higher rate, LH levels plummeted to undetectable concentrations during the first 2 h of CSF exfusion and remained low throughout the collection period. Pituitary responsiveness was not reduced, as a GnRH bolus (0.25 or 2.5 micrograms) after 6 h of CSF removal elicited a dose-dependent stimulation of LH secretion. In contrast, a higher incidence of normal pulsatile LH secretion (12 of 19 collections) was observed when the CSF withdrawal rate was reduced. During these 12 collections, LH and GnRH pulses occurred at regular intervals and exhibited similar pulse frequencies (mean +/- SE, 0.76 +/- 0.07 and 0.67 +/- 0.09 pulses/h for LH and GnRH, respectively). Most GnRH and LH pulses were synchronized, as 86% of all GnRH pulses (43 of 50) were accompanied by a LH pulse. (ABSTRACT TRUNCATED AT 400 WORDS)
Compact 2.4GHz-band diversity antenna for mobile terminals

A compact diversity antenna adopting 3-D structures for mobile terminals is proposed and studied. The antenna consists of two asymmetric quasi-dipole elements, which are printed on the upper-board, and two side-boards. It has a -10 dB impedance bandwidth of 250 MHz (2.37-2.62 GHz), covering the 2.4GHz WLAN (2400-2483 MHz) and across the whole operating frequency band, the mutual coupling between the two elements is below -17dB. The evaluation of the diversity performance is also conducted by calculating the envelope correlation coefficient and the mean effective gains using the measured radiation pattern data.
hSulf1 Sulfatase promotes apoptosis of hepatocellular cancer cells by decreasing heparin-binding growth factor signaling.

BACKGROUND AND AIMS The heparin-binding growth factors fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) are potent mitogens for hepatocellular carcinomas (HCCs). Heparin-binding growth factor signaling is regulated by sulfation of cell-surface heparan sulfate proteoglycans (HSPGs). We hypothesized that hSulf1, a recently described sulfatase, regulates growth signaling in HCCs. METHODS Expression of hSulf1 in human HCC tumors was determined by real-time PCR. Down-regulation of hSulf1 expression was investigated by analyzing loss of heterozygosity (LOH) at the hSulf1 locus and the effect of the DNA methylation inhibitor 5-aza-deoxycytidine on hSulf1 expression. The subcellular location of hSulf1 and sulfation state of cell-surface HSPGs were assessed by immunocytochemistry. FGF and HGF signaling was examined by phospho-specific immunoblot analysis. Cell growth was measured by trypan blue exclusion, and the MTT assay and apoptosis were quantitated by fluorescence microscopy. RESULTS hSulf1 expression was decreased in 29% of HCCs and 82% of HCC cell lines. There was LOH at the hSulf1 locus in 42% of HCCs. Treatment with 5-aza-deoxycytidine reactivated hSulf1 expression in hSulf1-negative cell lines. Low hSulf1-expressing cells showed increased sulfation of cell-surface HSPGs, enhanced FGF and HGF-mediated signaling, and increased HCC cell growth. Conversely, forced expression of hSulf1 decreased sulfation of cell-surface HSPGs and abrogated growth signaling. HCC cells with high-level hSulf1 expression were sensitive to staurosporine- or cisplatin-induced apoptosis, whereas low expressing cells were resistant. Transfection of hSulf1 into hSulf1-negative cells restored staurosporine and cisplatin sensitivity. CONCLUSIONS Down-regulation of hSulf1 contributes to hepatocarcinogenesis by enhancing heparin-binding growth factor signaling and resistance to apoptosis.
Self report on quality of life in dementia with modified COOP/WONCA charts

This study investigates whether a modified version of the COOP/WONCA charts is suitable to assess quality of life (QOL) in persons with dementia in nursing homes. A group of 112 institutionalized persons with moderate to severe dementia were approached for an interview. Twenty-two were observed not to be communicative, leaving 90. Sixty-seven persons were able to answer four out of six questions adequately (interviewable). Inter-observer reliability (n=38) was excellent (weighted kappa 0.90 to 0.97). Test-retest reliability (n=34; one week interval) ranged from poor for Daily and Social Activities and for the QOL charts, to moderate for Feelings and Pain, and satisfactory for Physical Functioning (weighted kappa 0.23 to 0.67). Interviewability was associated with severity of the dementia and communication ability. Support for convergent validity was found in medium-sized Spearman correlations between the COOP/WONCA charts and related variables. Support for discriminant validity was found in the absence of association between the Ccharts and non-related variables. The modified COOP/WONCA charts can be used to assess QOL in 60% of people with dementia in nursing homes but further modification is needed. Severe cognitive impairment and communication disabilities proved limiting factors for the use of the instrument. Although the illustrations on the charts appeared not to be helpful, the written response options in addition to verbal presentation proved useful during the administration of the charts.