Miracle-dz/UniFAR-FTUs huggingface dataset:
Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge

Rapid polymerization of a network of short, branched actin filaments takes place at the leading edge of migrating cells, a compartment enriched in activators of actin polymerization such as the Arp2/3 complex and cofilin. Actin filaments elsewhere in the cell are long and unbranched. Results reported here show that the presence or absence of tropomyosin in these different actin-containing regions helps establish functionally distinct actin-containing compartments in the cell. Tropomyosin, an inhibitor of the Arp2/3 complex and cofilin function, was localized in relation to actin filaments, the Arp2/3 complex, and free barbed ends of actin filaments in MTLn3 cells, which rapidly extend flat lamellipodia following EGF stimulation. All tropomyosin isoforms examined using indirect immunofluorescence were relatively absent from the dynamic leading edge compartment, but did colocalize with actin structures deeper in the lamellipodium and in stress fibers. An in vitro light microscopy assay revealed that tropomyosin protects actin filaments from cofilin severing. The results suggest that tropomyosin-free actin filaments under the membrane can participate in rapid, dynamic processes that depend on interactions between the activities of the Arp2/3 complex and ADF/cofilin that tropomyosin inhibits elsewhere in the cell.
Towards the isomer-specific synthesis of higher fullerenes and buckybowls by the surface-catalyzed cyclodehydrogenation of aromatic precursors.

Keywords: cyclodehydrogenation ; fullerenes ; heterogeneous catalysis ; nanotechnology ; surface chemistry ; Chemical-Synthesis ; C-60 Adsorption ; Transition Reference EPFL-ARTICLE-172115doi:10.1002/anie.201005000View record in Web of Science Record created on 2011-12-16, modified on 2017-05-12
CREB/ATF-Dependent Repression of Cyclin A by Human T-Cell Leukemia Virus Type 1 Tax Protein

ABSTRACT Expression of the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax is correlated with cellular transformation contributing to the development of adult T-cell leukemia. Tax has been shown to modulate the activities of several cellular promoters. Existing evidence suggests that Tax need not directly bind to DNA to accomplish these effects but rather that it can act through binding to cellular factors, including members of the CREB/ATF family. Exact mechanisms of HTLV-1 transformation of cells have yet to be fully defined, but the process is likely to include both activation of cellular-growth-promoting factors and repression of cellular tumor-suppressing functions. While transcriptional activation has been well studied, transcriptional repression by Tax, reported recently from several studies, remains less well understood. Here, we show that Tax represses the TATA-less cyclin A promoter. Repression of the cyclin A promoter was seen in both ts13 adherent cells and Jurkat T lymphocytes. Two other TATA-less promoters, cyclin D3 and DNA polymerase α, were also found to be repressed by Tax. Interestingly, all three promoters share a common feature of at least one conserved upstream CREB/ATF binding site. In electrophoretic mobility shift assays, we observed that Tax altered the formation of a complex(es) at the cyclin A promoter-derived ATF site. Functionally, we correlated removal of the CREB/ATF site from the promoter with loss of repression by Tax. Furthermore, since a Tax mutant protein which binds CREB repressed the cyclin A promoter while another mutant protein which does not bind CREB did not, we propose that this Tax repression occurs through protein-protein contact with CREB/ATF.
ASYMMETRIC VALUATION OF GAINS AND LOSSES AND PREFERENCE ORDER ASSUMPTIONS

Recent findings of large differences between people's valuations of gains and losses imply that the common preference order axioms of completeness, transitivity, and dominance on which economic analyses and predictions of consumer behavior are largely based, may not be consistent with actual choices. This paper reports the results of an experimental test of the preference order assumptions using real exchanges of two goods and money. The findings show consistent differences in the valuation placed on goods and money, depending on whether the entitlements are being acquired or given up, and provide direct evidence of preference assumption violations. Copyright 1995 by Oxford University Press.
Marketing Strategy, Related Factors and Performance of Firms Across Saudi Arabia

This study conducted in Saudi Arabia, examines the degree of emphasis laid by manufacturing firms on their marketing strategy and other functional strategies. The study also investigates the relationship between marketing strategy and firm's performance. Further investigations include an examination of the differences on the emphasis on marketing strategy and the contextual factor such as its size, type of goods and market type. The study employs a survey methodology; self-administered questionnaires were distributed to a sample of respondents in the three major cities representing the three major provinces of Saudi Arabia. The study findings provide useful insights into the relative importance given by the manufacturing firms in Saudi Arabia to marketing strategy vis a vis other functional strategies. Findings of this study are intended to help firms to have a better understanding and formulate effective strategies.
The ‘Policy Mix’ for Innovation: Rethinking Innovation Policy in a Multi-Level, Multi-Actor Context

Recent years have seen the emergence, take-up and use of the term 'policy mix' by innovation policy makers and by policy analysts & scholars alike. Imported from economic policy debates, the term implies a focus on the interactions and interdependencies between different policies as they affect the extent to which intended policy outcomes are achieved. However the meaning of the term remains ambiguous. Nonetheless, we argue that the emergence of the „policy mix‟ concept into common use in the field of innovation policy studies provides us with a window of opportunity to reconsider some basic and often hidden assumptions in order to better deal with a messy and complex, multi-level, multi-actor reality. We draw on the mainstream policy studies literature and on evolutionary thinking in order to re-conceptualise the basic building blocks of innovation policy studies in order to arrive at a useful definition of ‟policy mix‟ interactions. We suggest that this reconceptualisation has profound implications for the scope and focus of innovation policy studies and for what such studies can realistically hope to achieve in terms of policy prescriptions.
Parasaccharibacter apium, gen. nov., sp. nov., Improves Honey Bee (Hymenoptera: Apidae) Resistance to Nosema

Abstract The honey bee, Apis mellifera L. , is host to a variety of microorganisms. The bacterial community that occupies the adult worker gut contains a core group of approximately seven taxa, while the hive environment contains its own distribution of bacteria that is in many ways distinct from the gut. Parasaccharibacter apium, gen. nov., sp. nov., is a hive bacterium found in food stores and in larvae, worker jelly, worker hypopharyngeal glands, and queens. Parasaccharibacter apium increases larval survival under laboratory conditions. To determine if this benefit is extended to colonies in the field, we tested if P. apium 1) survives and reproduces in supplemental pollen patty, 2) is distributed throughout the hive when added to pollen patty, 3) benefits colony health, and 4) increases the ability of bees to resist Nosema. Parasaccharibacter apium survived in supplemental diet and was readily consumed by bees. It was distributed throughout the hive under field conditions, moving from the pollen patty to hive larvae. While P. apium did not significantly increase colony brood production, food stores, or foraging rates, it did increase resistance to Nosema infection. Our data suggest that P. apium may positively impact honey bee health.
Influence Of Interpersonal Trust On Innovative Behaviour Of Service Workers: Mediating Effects Of Knowledge Sharing

This study investigated the effects of interpersonal trust among service workers (i.e., trust in co-workers and supervisors) on innovative behaviour and examined the mediating effect of knowledge sharing on these relationships. A total of 188 personal trainers from 11 fitness clubs participated in this study. The results revealed that both trust in co-workers and trust in supervisors have a positive effect on knowledge sharing. Although the effect of trust in co-workers on innovative behaviour was non-significant, trust in supervisors had a significant effect on innovative behaviour. The effect of employees’ knowledge sharing on innovative behaviour was also found to be significant. Additionally, the results indicated that knowledge sharing has a full mediating effect on the relationship between trust in co-workers and innovative behaviour and a partial mediating effect on the relationship between trust in supervisors and innovative behaviour.
Mechanistic Insight into Trimethylamine N-Oxide Recognition by the Marine Bacterium Ruegeria pomeroyi DSS-3

ABSTRACT Trimethylamine N-oxide (TMAO) is an important nitrogen source for marine bacteria. TMAO can also be metabolized by marine bacteria into volatile methylated amines, the precursors of the greenhouse gas nitrous oxide. However, it was not known how TMAO is recognized and imported by bacteria. Ruegeria pomeroyi DSS-3, a marine Roseobacter, has an ATP-binding cassette transporter, TmoXWV, specific for TMAO. TmoX is the substrate-binding protein of the TmoXWV transporter. In this study, the substrate specificity of TmoX of R. pomeroyi DSS-3 was characterized. We further determined the structure of the TmoX/TMAO complex and studied the TMAO-binding mechanism of TmoX by biochemical, structural, and mutational analyses. A Ca2+ ion chelated by an extended loop in TmoX was shown to be important for maintaining the stability of TmoX. Molecular dynamics simulations indicate that TmoX can alternate between “open” and “closed” states for binding TMAO. In the substrate-binding pocket, four tryptophan residues interact with the quaternary amine of TMAO by cation-π interactions, and Glu131 forms a hydrogen bond with the polar oxygen atom of TMAO. The π-π stacking interactions between the side chains of Phe and Trp are also essential for TMAO binding. Sequence analysis suggests that the TMAO-binding mechanism of TmoX may have universal significance in marine bacteria, especially in the marine Roseobacter clade. This study sheds light on how marine microorganisms utilize TMAO. IMPORTANCE Trimethylamine N-oxide (TMAO) is an important nitrogen source for marine bacteria. The products of TMAO metabolized by bacteria are part of the precursors of the greenhouse gas nitrous oxide. It is unclear how TMAO is recognized and imported by bacteria. TmoX is the substrate-binding protein of a TMAO-specific transporter. Here, the substrate specificity of TmoX of Ruegeria pomeroyi DSS-3 was characterized. The TMAO-binding mechanism of TmoX was studied by biochemical, structural, and mutational analyses. Moreover, our results suggest that the TMAO-binding mechanism may have universal significance in marine bacteria. This study sheds light on how marine microorganisms utilize TMAO and should lead to a better understanding of marine nitrogen cycling.
A randomized-controlled trial of cognitive–behavioral therapy for depression with integrated techniques from emotion-focused and exposure therapies

ABSTRACT Background: Emotional processing (EP) is hypothesized to be a key mechanism of change in psychotherapy that may enhance its long-term efficacy. To study the effects of fostering EP in psychotherapy for depression, this randomized-controlled clinical trial compares the efficacy and pattern of change of a cognitive–behavioral therapy that integrates emotion-focused techniques within an exposure framework (Exposure-Based Cognitive Therapy for depression; EBCT-R) to a standard cognitive–behavioral therapy (CBT). Methods: One hundred and forty-nine depressed outpatients were randomized to a maximum of 22 sessions of manualized EBCT-R (N = 77) or CBT (N = 72). Primary outcomes were self-reported and clinician-rated depressive symptoms at posttreatment and 12-month follow-up. Secondary outcomes were self-esteem, interpersonal problems, and avoidance thoughts and behaviors. Results: Depressive symptoms improved significantly over therapy in both treatments, with large within-group effect sizes for CBT (d = −1.95) and EBCT-R (d = −1.77). The pattern of depression change during treatment did not differ between treatments. Symptom relief lasted over 12 months and did not differ between EBCT-R and CBT. Conclusions: Results suggest that both treatments produced significant short- and long-term improvement in depression symptoms, but the integration of emotion-focused techniques within an exposure framework did not have added benefit. Trial registration: ClinicalTrials.gov Identifier: NCT01012856 Clinical or methodological significance of this article: This trial compares cognitive–behavioral therapy (CBT) with a similarly structured CBT that was designed to foster emotional processing by integrating emotion-focused techniques within an exposure framework. Results indicate that this form of assimilative integration did not improve outcomes at 12-month follow-up.
Institutional Ownership and Income Smoothing: Australian Evidence

This study examines the rarely investigated association between institutional ownership and income smoothing. The results support the predicted positive association between institutional ownership and the likelihood of firms smoothing earnings towards their earnings trend in general. However, this association is not systematic across all firms. The positive association is most evident among profit firms with pre-managed earnings above their earnings trend. No significant association is found for profit firms with pre-managed earnings below their earnings trend and loss firms in general. This study also finds that, in Australia, while institutional ownership has a non-linear association with income increasing earnings management (Koh, 2003), such association manifests itself within the income smoothing framework. The results of this study highlight the complexities in the association between institutional ownership and earnings management strategies, and future research can benefit by explicitly examining the trade-offs between alternative earnings management incentives and the factors that affect the relative strength of these incentive trade-offs.
Extracellular matrix and the kidney.

Introduction Histopathologists have long recognised that excessive accumulation of extracellular matrix in the kidney is a serious sign of irreversible damage. However, we have rarely attempted further analysis of such accumulations, beyond noting the presence of pink staining material in our haematoxylin and eosin sections, and a rough estimation of its severity. This is remiss of us, as fibrosis of the renal interstitium has repeatedly been reported to be the best single morphological correlate of renal function and prognosis. 2 The purpose of this review is to summarise selected recent developments in this field, and to consider how these developments may alter the ways in which histopathologists might assess the extracellular matrix. Although I will concentrate on the kidney, there are obvious parallels with the assessment of non-neoplastic disease in other organs.
Multicentre standardisation of a clinical grade procedure for the preparation of allogeneic platelet concentrates from umbilical cord blood.

BACKGROUND In addition to a largely prevalent use for bleeding prophylaxis, platelet concentrates from adult blood have also been used for many years to prepare platelet gels for the repair of topical skin ulcers. Platelet gel can be obtained by activation of fresh, cryopreserved, autologous or allogeneic platelet concentrates with calcium gluconate, thrombin and/or batroxobin. The high content of tissue regenerative factors in cord blood platelets and the widespread availability of allogeneic cord blood units generously donated for haematopoietic transplant but unsuitable for this use solely because of low haematopoietic stem cell content prompted us to develop a national programme to standardise the production of allogeneic cryopreserved cord blood platelet concentrates (CBPC) suitable for later preparation of clinical-grade cord blood platelet gel. MATERIALS AND METHODS Cord blood units collected at public banks with total nucleated cell counts <1.5×10(9), platelet count >150×10(9)/L and volume >50 mL, underwent soft centrifugation within 48 hours of collection. Platelet-rich plasma was centrifuged at high speed to obtain a CBPC with target platelet concentration of 800-1,200×10(9)/L, which was cryopreserved, without cryoprotectant, below -40 °C. RESULTS During 14 months, 13 banks produced 1,080 CBPC with mean (± standard deviation) volume of 11.4±4.4 mL and platelet concentration of 1,003±229×10(9)/L. Total platelet count per CBPC was 11.3±4.9×10(9). Platelet recovery from cord blood was 47.7±17.8%. About one-third of cord blood units donated for haematopoietic transplant could meet the requirements for preparation of CBPC. The cost of preparation was € 160.92/CBPC. About 2 hours were needed for one technician to prepare four CBPC. DISCUSSION This study yielded valuable scientific and operational information regarding the development of clinical trials using allogeneic CBPC.
Signal transmission in the catfish retina. V. Sensitivity and circuit.

1. We analyzed the light-evoked responses of retinal neurons by means of a white-noise technique. Horizontal and bipolar cells produced a modulation response that was linearly related to a modulation of the mean luminance of a large field of light. The first-order kernels were capable of reproducing the cells' modulation response with a fair degree of accuracy. The amplitude as well as the waveform of the kernels changed with the change in the mean luminance. This is a parametric change and is a form of field adaptation. As the time constant of the parametric change was much longer than that of the modulation response (memory), neurons were assumed to be at a dynamic steady state at a given mean luminance. 2. With the presence of a steady annular illumination, the first-order kernel derived from stimulation with a small spot of light became faster in peak response time and larger in amplitude. For horizontal-cell somas and bipolar cells, the surround also linearized their modulation response. This surround enhancement has been seen in all the cone-driven retinal cells except the receptor and horizontal cell axon, in which a steady surround decreased the amplitude of the spot-evoked kernel but shortened the peak response time. 3. A change in the modulation depth did not affect either the amplitude or the wave-form of the first-order kernels from the horizontal and bipolar cells. In the amacrine and ganglion cells, on the other hand, the amplitude of kernels was related inversely to the depth of modulation. These cells were more sensitive to the modulation of a small modulation depth. 4. A static nonlinearity appeared when signals were transmitted to the amacrine cells. The nonlinearity was first produced in the type-C amacrine cells by a process, which could be modeled by squaring the bipolar cell response. A gamut of more complex second-order nonlinearities found in type-N amacrine cells could be modeled by a band-pass filtering of the type-C cell response. Linear components in the bipolar cells and nonlinear components in the amacrine cells are encoded into spike trains in the ganglion cells. Thus, under our simple stimulus regimen, the ganglion cells transformed the results of the preganglionic signal processing into a spike train without much modification. 5. We propose a tentative diagram of the signal flow in the cone-driven catfish retinal neurons based on this and previous studies. (ABSTRACT TRUNCATED AT 400 WORDS)
Utopia unrealised: an evaluation of a consultancy to develop a national framework for police education and training to enhance frontline response to illicit drug problems in Australia

This dissertation presents an evaluation of a funded consultancy that was intended to bring about change in the education and training of police in Australia in response to illicit drugs. Sponsored by what was at the time known as the Commonwealth Department of Health and Aged Care, the ultimate goal of the consultancy was a national framework for police education and training to enhance frontline police response to illicit drug problems. The research used a case study design. Guba and Stufflebeam’s (1970) Context, Input, Process, and Product (CIPP) model was used to organise the presentation of a rich description of the design, development and implementation of the consultancy. Application of this framework enabled illumination of a number of issues related to social policy, change and innovation, and quality improvement processes. The study explores the role of education and training in organisational change and concludes that the potential of external consultancy activity to effect meaningful change in police education, training and practice is limited by a number of factors. Key findings of the study are that while a number of consultancy processes could have been enhanced, the primary determinants of the extent to which a change in police education and training will enhance frontline practice are contextual and conceptual factors. The study reveals that the response of frontline police to illicit drug use is influenced by multivariate factors. The findings of this study suggest that while frontline police are keen to provide solutions to a range of practice issues in response to illicit drug problems, they desire concrete strategies that are well defined and supported by management, consistent with policy and within the law. However, the complexity of police activity in response to illicit drugs; the dissonance between the conceptual frameworks of police and health agencies; and, resistance to what is perceived as externally initiated change in police practice, education and training; were found to be powerful inhibitors of an utopian attempt to enhance frontline police response to illicit drug problems. Using the metaphor of board games, the study concludes that the development of an education and training framework will be of little value in achieving enhanced frontline practice in response to illicit drug problems unless the criteria for enhanced response are made more explicit and seen to be congruent with both the conceptualisation and operationalisation of police roles and functions. Moreover, the study questions the mechanisms through which changes in policy are conceived, implemented and evaluated and highlights a need for greater congruence between evaluation frameworks and the nature of change.
Financial Crisis of 2007-2010

This paper discusses the causes and impacts of the financial crisis of 2007–2010 and examines the reforms aimed at the prevention of its recurrence. The causes to be discussed include housing and commodity bubbles, easy credit conditions, subprime lending, predatory lending, deregulation and lax regulation, incorrect risk pricing, collapse of the shadow banking system and systemic risk. The impacts to be examined include the major financial institutions, the financial wealth, the economies of the U.S. and other countries — Iceland, Hungary, Russia, Spain, Ukraine, Dubai, and Greece. The paper further discusses emergency policy responses, principles of financial reforms and various regulatory proposals. The Dodd-Frank Wall Street Reform and Consumer Protection Act of 2010 and the Basel III accord are also discussed. Concluding remarks provide a brief discussion of the latest economic problems in 2011.