Miracle-dz/UniFAR-FTUs huggingface dataset:
Maximum intraoperative elongation of the rat sciatic nerve with tissue expander: Functional, neurophysiological, and histological assessment

The purpose of this study was to assess the maximum rapid intraoperative elongation of the rat sciatic nerve with the use of tissue expander, and its possible functional recovery. One hundred and eight rats were divided into five groups, and their right sciatic nerves were expanded with a 10‐cc, 12‐cc, 14‐cc, 16‐cc, and 18‐cc expander, respectively, for 1 h. The functional recovery of the nerve was assessed at intervals up to 3 months, using the sciatic function index (SFI), neurophysiological indices, and histology. The maximum intraoperative elongation was observed in group IV (16‐cc volume of tissue expander), at about 23.83%. SFI decreased between the first and seventh postoperative days, but gradually recovered, reaching preoperative values in all groups according to the formulas of De Medinaceli et al. (Exp. Neurol. 77:634–643, 1982) and Bain et al. (Plast. Reconstr. Surg. 83:129–136, 1989). Latency and motor conduction velocity demonstrated deterioration after expansion, which peaked after surgery. Recovery was gradually completed by the end of the experiment. The histological findings indicated minor aberrations immediately after expansion and maximal demyelination with axonal disruption on day 15. The reparative process started by day 30 and continued until day 90, when almost no histological changes were observed. In conclusion, intraoperative nerve expansion successfully elongates the rat sciatic nerve up to 23.83%. But it causes functional and morphological abnormalities, which are of moderate to severe degree, are of short duration, and are reversible. Intraoperative nerve expansion might be a valuable solution in the treatment of short nerve gaps, but its clinical application still needs to be evaluated. © 2006 Wiley‐Liss, Inc. Microsurgery, 2006
Involvement of Nitrogen-Containing Compounds in β -Lactam Biosynthesis and its Control

ABSTRACT Biosynthesis of β -lactam antibiotics by fungi and actinomycetes is markedly affected by compounds containing nitrogen. The different processes employed by the spectrum of microbes capable of making these valuable compounds are affected differently by particular compounds. Ammonium ions, except at very low concentrations, exert negative effects via nitrogen metabolite repression, sometimes involving the nitrogen regulatory gene nre. Certain amino acids are precursors or inducers, whereas others are involved in repression and, in certain cases, as inhibitors of biosynthetic enzymes and of enzymes supplying precursors. The most important amino acids from the viewpoint of regulation are lysine, methionine, glutamate and valine. Surprisingly, diamines such as diaminopropane, putrescine and cadaverine induce cephamycin production by actinomycetes. In addition to penicillins and cephalosporins made by fungi and cephamycins made by actinomycetes, other β-lactams are made by actinomycetes and unicellular bacteria. These include clavams (e.g., clavulanic acid), carbapenems (e.g., thienamycin), nocardicins and monobactams. Here also, amino acids are precursors and inhibitors, but only little is known about regulation. In the case of the simplest carbapenem made by unicellular bacteria, i.e., 1-carba-2-em-3-carboxylic acid, quorum sensors containing homoserine lactone are inducers.
A novel method for camera calibration based on micro plane grid

The ordinary digital camera calibration has been one of the active research fields in computer vision and photogrammetry. This paper proposes a novel calibration method with ease for operation and high accuracy, as well as robustness. This method uses a micro plane grid as the calibration reference object which is photographed for a couple of images in the same direction, applies a high-precision distortion model which takes the high-order nonlinear distortion into account, and restrains the correlation effects among camera parameters so as to strengthen the convergence of the algorithm by iterative calculation of distortion coefficients and interior orientation elements in steps. The experiments demonstrate stable results and reliable accuracy of calibration is achieved by this method.
Immune or inflammatory response by the host brain suppresses neuronal differentiation of transplanted ES cell–derived neural precursor cells

Embryonic stem (ES) cells are a promising donor source for transplantation therapy, but several problems must be solved before they can be clinically useful. One of these is the host immune reaction to allogeneic grafts. In this article, we examine the effect of the host immune reaction on survival and differentiation of grafted ES cell–derived neural precursor cells (NPCs). We induced NPCs from mouse ES cells by stromal cell–derived inducing activity and then transplanted them into mouse brains with or without administering the immunosuppressant cyclosporine A (CsA). Two and 8 weeks following transplantation, the accumulation of host‐derived microglia/macrophages and lymphocytes was observed around the graft. This effect was reduced by CsA treatment, although no significant difference in graft volume was observed. These data suggest that an immune response occurs in allografts of ES cell–derived NPCs. Intriguingly, however, the ratio of neurons to astrocytes in the graft was higher in immunosuppressed mice. Because inflammatory or immune cells produce various cytokines, we examined the effect of IL‐1β, IL‐6, IFN‐γ, and TNF‐α on the differentiation of NPCs in vitro. Only IL‐6 promoted glial cell fate, and this effect could be reversed by the addition of an IL‐6 neutralizing antibody. These results suggest that allogeneic ES cell–derived NPCs can cause an immune response by the host brain, but it is not strong enough to reject the graft. More important, activated microglia and lymphocytes can suppress neuronal differentiation of grafted NPCs in vivo by producing cytokines such as IL‐6. © 2008 Wiley‐Liss, Inc.
Insect Repellents, Phenoxyherbicide Exposure, and Non-Hodgkin's Lymphoma

Objective: We sought to test a hypothetical explanation of contradictory results in studies of phenoxyherbicides and NHL, that the exposure of rubber gloves recommended for use by farmers when mixing or applying pesticides simultaneously to 2,4-D (2,4-dichlorophenoxyacetic acid), DEET (N,N-diethyl-m-toluamide), and ultraviolet rays increased their permeability to 2,4-D. Methods: We conducted a case (NHL n = 513)/control (n = 1506) study among men using age; province of residence; exposure to insect repellents containing DEET, phenoxy-herbicides, or dicamba; and gloves when handling pesticides. Results: Using conditional logistic regression, the stratum with reported exposure to mecoprop, to DEET and the use of rubber gloves had higher odds ratios (3.86; 95% confidence interval = 1.57–9.49) compared with strata with other combinations. Conclusions: In conclusion, the etiologic complexity of NHL was demonstrated.
The Varicella-Zoster Virus (VZV) ORF9 Protein Interacts with the IE62 Major VZV Transactivator

ABSTRACT The varicella-zoster virus (VZV) ORF9 protein is a member of the herpesvirus UL49 gene family but shares limited identity and similarity with the UL49 prototype, herpes simplex virus type 1 VP22. ORF9 mRNA is the most abundantly expressed message during VZV infection; however, little is known concerning the functions of the ORF9 protein. We have found that the VZV major transactivator IE62 and the ORF9 protein can be coprecipitated from infected cells. Yeast two-hybrid analysis localized the region of the ORF9 protein required for interaction with IE62 to the middle third of the protein encompassing amino acids 117 to 186. Protein pull-down assays with GST-IE62 fusion proteins containing N-terminal IE62 sequences showed that amino acids 1 to 43 of the acidic transcriptional activation domain of IE62 can bind recombinant ORF9 protein. Confocal microscopy of transiently transfected cells showed that in the absence of other viral proteins, the ORF9 protein was localized in the cytoplasm while IE62 was localized in the nucleus. In VZV-infected cells, the ORF9 protein was localized to the cytoplasm whereas IE62 exhibited both nuclear and cytoplasmic localization. Cotransfection of plasmids expressing ORF9, IE62, and the viral ORF66 kinase resulted in significant colocalization of ORF9 and IE62 in the cytoplasm. Coimmunoprecipitation experiments with antitubulin antibodies indicate the presence of ORF9-IE62-tubulin complexes in infected cells. Colocalization of ORF9 and tubulin in transfected cells was visualized by confocal microscopy. These data suggest a model for ORF9 protein function involving complex formation with IE62 and possibly other tegument proteins in the cytoplasm at late times in infection.
Effect of Tinospora Cordifolia on the Antitumor Activity of Tumor-Associated Macrophages–Derived Dendritic Cells

We and others previously have reported that extract prepared from medicinal plant Tinospora cordifolia shows a wide spectrum of immunoaugmentary effects. Tinospora cordifolia was shown to upregulate antitumor activity of tumor-associated macrophages (TAM). In this article we present evidence to show that an alcoholic extract of Tinospora cordifolia (ALTC) enhances the differentiation of TAM to dendritic cells (DC) in response to granulocyte/macrophage-colony-stimulating factor, interleukin-4, and tumor necrosis factor. DC differentiated in vitro from TAM that were harvested from tumor-bearing mice after i.p. administration of ALTC (200 mg/kg body weight) 2 days posttumor transplantation shows an enhanced tumor cytotoxicity and production of tumoricidal soluble molecules like TNF, IL-1, and NO. Adoptive transfer of these TAM-derived DC to Dalton's lymphoma-bearing mice resulted in prolongation of survival of tumor-bearing mice. This is the first report regarding the differentiation and antitumor functions of TAM-derived DC obtained from tumor-bearing host administered with ALTC. The possible mechanisms involved also are discussed.
Analyzing Food-Related Life Satisfaction and other Predictors of Life Satisfaction in Central Chile

Abstract This study aimed to assess the effect of satisfaction with food-related life on life satisfaction among inhabitants of the main municipalities of central Chile. A survey was applied to a sample of 1,277 people, distributed proportionally by municipality. The questionnaire included the following scales: SWLS (Satisfaction with Life Scale), SWFL (Satisfaction with Food-related Life) and the Health-Related Quality of Life Index (HRQOL). Questions were asked regarding eating habits inside and outside the home, time available for meals at home, the assessment of five sources of happiness and the demographic characteristics of those surveyed. An ordered logit model was proposed, in which the dependent variable was satisfaction with life. Satisfaction with life was significantly related to the respondent’s socioeconomic status, self-perception of health, degree of satisfaction with food-related life, monthly food expenditure, time available for supper with the family (p < .01); gender, self-reported number of days affected by mental health problems, frequency of supper with the family, the degree of agreement with respect to family being an important source of happiness (p < .05); and family size and frequency of food consumption in fast food outlets (p < .10). Satisfaction with life in the study sample is related to aspects associated with health, family and eating, and the family interaction associated with eating may play an important role in overall satisfaction with life.
Systemic infections mimicking thrombotic thrombocytopenic purpura

The absence of specific diagnostic criteria, the urgency to begin plasma exchange treatment, and the risk for complications from plasma exchange make the initial evaluation of patients with suspected thrombotic thrombocytopenic purpura (TTP) difficult. Systemic infections may mimic the presenting clinical features of TTP. In the Oklahoma TTP‐HUS (hemolytic‐uremic syndrome) Registry, 1989–2010, 415 consecutive patients have been clinically diagnosed with their first episode of TTP; in 31 (7%) the presenting clinical features were subsequently attributed to a systemic infection. All 31 patients had diagnostic criteria for TTP; 16 (52%) had the complete “pentad” of microangiopathic hemolytic anemia, thrombocytopenia, neurologic abnormalities, renal failure, and fever. Four (16%) of 25 patients who had ADAMTS13 measurements had <10% activity; three patients had a demonstrable ADAMTS13 inhibitor. Compared with 62 patients with severe ADAMTS13 deficiency (<10%) who had no recognized alternative disorders, patients with systemic infections had more frequent fever, coma, renal failure, and the complete “pentad” of clinical features. Seventeen different infectious etiologies were documented. A systematic literature review identified 67 additional patients with a diagnosis of TTP or HUS and also a systemic infection. Among all 98 patients, infections with 41 different bacteria, viruses, and fungi were documented, suggesting that many different systemic infections may mimic the presenting clinical features of TTP. Initial plasma exchange treatment is appropriate in critically ill patients with diagnostic features of TTP, even if a systemic infection is suspected. Continuing evaluation to document a systemic infection is essential to determine the appropriateness of continued plasma exchange. Am. J. Hematol. 86:743–751, 2011. © 2011 Wiley‐Liss, Inc.
TAKING THE STANCE: ON THE IMPORTANCE OF REFLECTIVE PRACTICE PERCIEVED BY TEACHER EDUCATORS. CLARIFYING THE ROLE OF TEACHER EDUCATORS

Nowadays, the phenomenon of reflective practice has gained wide recognition in higher education institutions preparing pre-service teachers for their future careers. As research shows, the capability of reflective practice is developmental and highly depends on the attitudes and practice employed by both student teachers and their educators. Guided by these assumptions, the current paper attempts to reveal the role of teacher educators in facilitating reflective practice of pre-service teachers. The main roles of teacher educators as facilitators are disclosed by employing the method of scientific literature review.
The Threefold Protrusions of Adeno-Associated Virus Type 8 Are Involved in Cell Surface Targeting as Well as Postattachment Processing

ABSTRACT Adeno-associated virus (AAV) has attracted considerable interest as a vector for gene therapy owing its lack of pathogenicity and the wealth of available serotypes with distinct tissue tropisms. One of the most promising isolates for vector development, based on its superior gene transfer efficiency to the liver in small animals compared to AAV type 2 (AAV2), is AAV8. Comparison of the in vivo gene transduction of rAAV2 and rAAV8 in mice showed that single amino acid exchanges in the 3-fold protrusions of AAV8 in the surface loops comprised of residues 581 to 584 and 589 to 592 to the corresponding amino acids of AAV2 and vice versa had a strong influence on transduction efficiency and tissue tropism. Surprisingly, not only did conversion of AAV8 to AAV2 cap sequences increase the transduction efficiency and change tissue tropism but so did the reciprocal conversion of AAV2 to AAV8. Insertion of new peptide motifs at position 590 in AAV8 also enabled retargeting of AAV8 capsids to specific tissues, suggesting that these sequences can interact with receptors on the cell surface. However, a neutralizing monoclonal antibody that binds to amino acids 588QQNTA592 of AAV8 does not prevent cell binding and virus uptake, indicating that this region is not necessary for receptor binding but rather that the antibody interferes with an essential step of postattachment processing in which the 3-fold protrusion is also involved. This study supports a multifunctional role of the 3-fold region of AAV capsids in the infection process.
Output power leveling of wind turbine Generator for all operating regions by pitch angle control

Wind energy is not constant and windmill output is proportional to the cube of wind speed, which causes the generated power of wind turbine generators (WTGs) to fluctuate. In order to reduce fluctuation, different methods are available to control the pitch angle of blades of windmill. In a previous work, we proposed the pitch angle control using minimum variance control, and output power leveling was achieved. However, it is a controlled output power for only rated wind speed region. This paper presents a control strategy based on average wind speed and standard deviation of wind speed and pitch angle control using a generalized predictive control in all operating regions for a WTG. The simulation results by using actual detailed model for wind power system show the effectiveness of the proposed method.
Body height and weight of children in Novi Sad

Summary. Background: Height and weight are the most important measures of growth and development and indirect indicators of living conditions. The socio-economic conditions in Serbia have varied drastically. In 1990, a political and economic crisis started causing a rapid fall of standards of living. Aim: The study aimed to determine the height and weight of children aged 3–11 in the periods between 1971, 1981, 1991 and 2001 in Novi Sad, Serbia. Subjects and methods: This investigation was carried out in the same primary schools and pre-school institutions. The data included children of Serbian nationality born in Novi Sad whose parents were also born there and had the same socio-economic background. Results: Positive changes in height and weight were recorded in the decades 1971–1981 and 1981–1991, except for the height of 8-year-old children. In the period 1991–2001, an increase in height was observed only at the age of 8 in boys and until the age of 9 in girls. As for weight, an increase was recorded in 9-year-old boys, while in girls it was present at all ages except for the ages of 7 and 10. Considering the period 1971–2001, positive changes in height were recorded from the age of 6 in boys and 5 in girls. The changes in weight were positive at all ages except the age of 5 in boys and after the age of 6 in girls. Conclusion: Lower values of height and weight recorded in 2001 are probably due to the changes in living conditions or they indicate that the acceleration reached its peak.
Power Comparison of Multivariate Wilcoxon-type Tests Based on the Jurečková–Kalina’s Ranks of Distances

A multivariate two-sample testing problem is one of the most important topics in nonparametric statistics. One of the multivariate two-sample testing problems based on the Jurečková–Kalina ranks of distance is discussed in this article. Further, a multivariate Wilcoxon-type test is proposed for testing the equality of two continuous distribution functions. Simulations are used to investigate the power of this test for the two-sided alternative with various population distributions. The results show that the proposed test statistic is more suitable than various existing statistics for testing a shift in the locationt and location-scale parameters.
Quality improvement in pediatric surgery

Purpose of review The goal of this review is to provide updates on the evolution of conceptual definitions as they relate to quality in healthcare, existing measurement platforms for performance benchmarking in pediatric surgery, and available tools for quality improvement that are relevant to care of the pediatric surgical patient. Recent findings The American College of Surgeon's National Surgical Quality Improvement Program-Pediatric has continued to evolve, now providing risk-adjusted safety outcomes data to over 70 hospitals and broadening its scope of quality measurement to include resource utilization and value-based metrics. Increasing use of checklists and other team-based communication tools show potential for making surgical care safer for children, and thoughtful application of quality improvement methods such as Lean methodology, six-sigma and others are helping to improve efficiency and increase healthcare value. Finally, efforts to define minimal resource standards for pediatric surgical care holds promise to improve outcomes for neonates and other children with complex surgical needs. Summary Over the past decade, significant progress has been made in our ability to measure, benchmark and improve quality in pediatric surgery. Future efforts will need to facilitate broader participation in benchmarking programs and knowledge-sharing collaboratives, and to develop multidisciplinary, ‘disease-specific’ longitudinal care models where quality measurement extends before and beyond the ‘traditional’ 30-day perioperative period.
Early Administration of High-Dose Antithrombin in Severe Sepsis: Single Center Results from the KyberSept-Trial

BACKGROUND: The overall finding in the KyberSept trial of no treatment effect of high-dose antithrombin (AT) in severe sepsis was inconsistent for the primary outcome, 28-day mortality, possibly because of patient heterogeneity. No data have been reported on the effects of AT therapy administered early in severe sepsis when microcirculation is disturbed but irreversible organ damage has not yet developed. OBJECTIVE: We report the post hoc results of the KyberSept trial in patients with severe sepsis treated at a single center early after new onset organ failure. METHODS: All study participants from a United States tertiary care intensive care unit were analyzed. Patients had been randomized 1:1 (placebo: n = 41; AT: n = 40) to receive AT (30,000 IU IV over a period of four days) or placebo within 48 h. RESULTS: Baseline variables were well balanced between groups. Eighty percent of patients (n = 65) received study drug within 24 h after onset of severe sepsis; 94% (n = 76) received study drug within 48 h. Nine of 40 participants in the AT group (22.5%) had new organ dysfunction during the first 7 days which was not present at baseline compared with 17 of 39 subjects (43.6%) in the placebo group (P = 0.058; two participants had dysfunction of all organs at baseline and were therefore excluded). At 28 days, 16 of 40 patients (40%) treated with AT died versus 22 of 41 (54%) with placebo [absolute reduction, 14%; odds ratio (95% confidence interval), 0.58 (0.24–1.39)]. In patients receiving AT, a significantly increased bleeding incidence was observed (any bleeding, 8 of 40 (20.0%) for AT group vs 1/41 (2.4%) for placebo group; P < 0.015). CONCLUSIONS: Data from this post hoc analysis confirm an increased bleeding risk seen with AT treatment in these patients. When given early in severe sepsis, though statistically not significant, absolute risk reductions with AT of 21% and 14% for organ failure and mortality, respectively, indicate a potential for treatment benefit in selected sepsis patients. This observation may have implications for continuing sepsis trials with AT that focus on reduced patient heterogeneity.