Miracle-dz/UniFAR-FTUs huggingface dataset:
New Mobilizable Vectors Suitable for Gene Replacement in Gram-Negative Bacteria and Their Use in Mapping of the 3′ End of theXanthomonas campestris pv. campestrisgum Operon

ABSTRACT We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3′ end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.
A cytoplasmic C-terminal fragment of syndecan-1 is generated by sequential proteolysis and antagonizes syndecan-1 dependent lung tumor cell migration

Syndecan-1 is a surface expressed heparan sulphate proteoglycan, which is upregulated by several tumor types and involved in tumor cell migration and metastasis. Syndecan-1 is shed from the cell surface and the remaining transmembrane fragment undergoes intramembrane proteolysis by γ-secretase. We here show that this generates a cytoplasmic C-terminal fragment (cCTF). In epithelial lung tumor A549 cells the endogenously produced cCTF accumulated when its proteasomal degradation was blocked with bortezomib and this accumulation was prevented by γ-secretase inhibition. Overexpression of the cCTF suppressed migration and invasion of A549 cells. This inhibitory effect was only seen when endogenous syndecan-1 was present, but not in syndecan-1 deficient cells. Further, overexpression of syndecan-1 cCTF increased the basal activation of Src kinase, focal adhesion kinase (FAK) and Rho GTPase. This was associated with increased adhesion to fibronectin and collagen G and an increased recruitment of paxillin to focal adhesions. Moreover, lung tumor formation of A549 cells in mice was reduced by overexpression of syndecan-1 cCTF. Finally, delivery of a synthetic peptide corresponding to the syndecan-1 cCTF suppressed A549 cell migration and increased basal phosphorylation of Src and FAK. Our data indicate that the syndecan-1 cCTF antagonizes syndecan-1 dependent tumor cell migration in vitro and in vivo by dysregulating proadhesive signaling pathways and suggest that the cCTF can be used as an inhibitory peptide.
Active ESD protection design against cross-power-domain ESD stresses in CMOS integrated circuits

New active ESD protection design for the interface circuits between separated power domains has been proposed and successfully verified in a 0.13-mum CMOS technology. The HBM and MM ESD robustness of the separated-power-domain interface circuits with the proposed active ESD protection design can achieve over 4 kV and 400 V, respectively.
Accelerating Time-Varying Hardware Volume Rendering Using TSP Trees and Color-Based Error Metrics

This paper describes a new hardware volume rendering algorithm for time-varying data. The algorithm uses the Time-Space Partitioning (TSP) tree data structure to identify regions within the data that have spatial or temporal coherence. By using this coherence, the rendering algorithm can improve performance when the volume data are larger than the texture memory capacity by decreasing the amount of textures required. This coherence can also allow improved speed by appropriately rendering flat-shaded polygons instead of textured polygons, and by not rendering transparent regions. To reduce the polygonization overhead caused by the use of the hierarchical data structure, we use a fast incremental polygon slicing algorithm. The paper also introduces new color-based error metrics, which more accurately identify coherent regions compared to the earlier scalar-based metrics. By showing experimental results from runs using different data sets and error metrics, we demonstrate that the new methods give substantial improvements in volume rendering performance.
Channel Estimation for PRP-OFDM in Underwater Acoustic Communication System

Orthogonal Frequency Division Multiplexing (OFDM) is a method that can combat multi-paths, fully use the frequency band, has high data rate and has a promising future in UWA communication. While longer the transmission range, worse the underwater conditions with multi-paths and Doppler offset, which needs to design the system parameters and take practical methods to improve the system. In this paper, a new PRP-OFDM (A Pseudo random Postfix OFDM) algorithm is proposed, which has a good performance and low-complexity.
MITRE TDT-2000 SEGMENTATION SYSTEM

We present t he design and d evelopment of a Hidden Markov Model for the division o f news broadcasts into story segments. Model t opology, and the textual features used, are discussed, together with the non-parametric estimation techniques that were employed for obtaining estimates for both transition and observation p robabilities. Visualization methods developed for the analysis of system performance are also presented.
The neural correlates of cue-induced craving in cocaine-dependent women.

OBJECTIVE Drug use reminders are associated with localized changes in brain activity related to intense drug wanting or craving in cocaine-dependent men. While cocaine dependence is prevalent and disabling in women, and certain clinically relevant sex differences exist, there is an absence of knowledge related to the neural correlates of cocaine craving in cocaine-dependent women. METHOD The differential neural response to imagery depicting cocaine use and neutral imagery was defined by using [15O]H2O positron emission tomography (PET) imaging in eight cocaine-dependent women. Results were compared with a matched group of eight cocaine-dependent men. RESULTS Cocaine-related imagery was associated with relative increases in cocaine craving and increases in regional cerebral blood flow in the superior temporal gyrus, dorsal anterior and posterior cingulate cortex, nucleus accumbens area, and the central sulcus. Compared with the results of an identical PET study in matched cocaine-dependent men, conditioned cocaine craving in women was associated with less activation of the amygdala, insula, orbitofrontal cortex, and ventral cingulate cortex and greater activation of the central sulcus and widely distributed frontal cortical areas. CONCLUSIONS These findings suggest the presence of sex differences in the functional anatomy of cue-induced cocaine craving associated with drug dependence. Such differences may reflect sex differences in conditioned associations to cocaine use, in affective and other corollaries of cocaine craving, or in their volitional regulation and may underlie apparent sex differences in the effects of cocaine abstinence and the expectations of treatment outcome. Some support for the need for sex-specific strategies for treatment of cocaine dependence is also furnished by the findings of this study.
TEACHING SPEAKING NARRATIVE MONOLOGUE BY USING SHRINKING STORY STRATEGY

Abstract: The purpose of this research is to find out the level of effectiveness of the use shrinking story strategy in teaching speaking narrative monologue to twelfth grade students of SMA Negeri 1 Semparuk in academic year 2013/2014. This research was a pre-experimental research with one group pre-test and post-test design. The population of this research were twelfth science grade students. The samples of this research were twelfth science one students. The technique of data collecting was measurement technique. The data were collected by applying pre-test and post-test through speaking narrative monologue performance orally and were analyzed by using speaking scoring criteria. The final computation shows that the effect size of the treatment is 1.55 (>0.8) and considered strong effect. It proves that the use of shrinking story strategy is highly-effective to teach speaking narrative monologue to twelfth science students. Key Words: Teaching, Narrative Monologue, Shrinking Story. Abstrak: penelitian ini bertujuan untuk menetahui tingkat keefektifan pengunaan strategy shrinking story dalam mengajarkan berbicara naratif dalam bentuk monolog kepada siswa kelas dua belas tahun ajaran 2013/2014. Penelitian ini adalah sebuah penelitian eksperimental dengan rancanggan satu grup pre-test dan post-test. Populasi dalam penelitian ini adalah siswa-siswa kelas dua belas ilmu alam. Sample penelitian ini adalah siswa kelas duabelas ilmu alam satu. Teknik pengumpulan data adalah teknik pengukuran. Data-data diambil dengan menerapkan pre-test dan post-test melalui penampilan berbicara naratif monolog secara lisan dan kemudian dianalisa menggunakan criteria penilaian berbicara. Perhitungan akhir menunjukkan bahwa effect size dari perlakuan adalah 1.55 (>0.8) dan dikatakan tinggi. Hal tersebut membuktikan bahwa penerapan strategy shrinking story sangat efektif untuk mengajar berbicara naratif monolog pada siswa kelas duabelas ilmu alam. Kata kunci: pengajaran, monolog naratif, shrinking story.
Management accounting change in South Africa

Most research on management accounting change relates to practices in developed countries. This paper reports on a field study of management accounting change in the South African context. It uses a contingency theory framework within four retail companies to understand the processes of their management accounting systems change and to explore the rationales for such change processes. The findings indicate considerable changes in management accounting systems within the four cases. Such changes include increased use of contemporary management accounting practices notably activity‐based cost allocation systems and the balanced scorecard approach to performance measures. The paper suggests that recent environmental changes in the South African economy arising from government reform/deregulation policy and global competition largely facilitated the management accounting change processes within the participating organisations.
Problem drug and alcohol use in a community sample of adolescents.

OBJECTIVE Epidemiological studies of illegal drug use in adolescents have examined frequency of drug use; a few have examined diagnoses or symptoms of drug abuse or dependence. This study examined problem use of illegal drugs, about which very little is known. METHOD Adolescents (879 boys and 929 girls), mean age of 15.7 years, representative of the province of Québec, Canada, were asked about problem use of alcohol and illegal drugs. RESULTS Almost one third had used illegal drugs more than 5 times. Of this group, more than 70% reported going to school high on drugs, and the majority reported playing sports while high and using drugs in the morning. In these drugs users, 94% of the boys and 85% of the girls reported at least 1 problem and two thirds of the boys and more than half of the girls reported 3 or more problems from illegal drugs. Marijuana was used by almost all subjects at the time of maximal drug use; hallucinogens were the second most commonly used drug. Alcohol was used more frequently than illegal drugs, but problem use was less common. CONCLUSIONS Problem drug use is the norm among the large minority who use illegal drugs more than a few times, and drug use is commonly incorporated into 2 major routine activities of teenagers--school and sports.
A multiple-focus graph browsing technique using heat models and force-directed layout

Describes a multiple-focus graph visualization and navigation technique using two heat models: a heat conduction model and a thermal radiation model. The proposed technique integrates force-directed layout techniques with the heat models. It provides virtual heat energy to focus nodes or a focus region. The heat influences the temperatures of link and other nodes, and then the temperatures determine the attributes, such as the sizes and lengths, of the nodes and links. A prototype browser using the proposed technique has been implemented with the Java programming language and the Java3D class library. The browser can visualize a general graph structure interactively in a 2D or 3D view. It allows us to have both multiple positive foci and negative foci, and enables us to navigate in a graph by using a heat-radiating pointer.
Proteomic analysis of phosphotyrosyl proteins in the rat brain: Effect of butorphanol dependence

Butorphanol (17‐cyclobutylmethyl‐3,14‐dihydroxymorphinan) tartrate (Stadol) is a mixed agonist‐antagonist opioid analgesic agent that is about five to seven times as potent as morphine in analgesic effects. The chronic use of butorphanol produces physical dependence in humans and animals. Phosphorylation plays a very important role in developing butorphanol dependence; however, global phosphorylation events induced by chronic butorphanol administration have not been reported. The aim of this study is to determine the alteration of tyrosine phosphorylation of brain frontal cortical proteins in butorphanol‐dependent rats using a proteomic approach. Dependence was produced by continuous intracerebroventricular (i.c.v.) infusion of butorphanol (26 nmol/μl/hr) for 72 hr via osmotic minipump in rats. Similar patterns of protein expression were detected by two‐dimensional electrophoresis (2‐DE) in brain frontal cortex of butorphanol‐dependent and saline‐treated control rats. All 65 phosphotyrosyl (p‐Tyr) protein spots detected in pH 3–10 phosphotyrosine 2‐DE of control rat brains were detected in butorphanol‐dependent rat brains. The densities of most p‐Tyr protein spots were increased in butorphanol‐dependent rat brains compared to saline‐treated control samples. Eighteen additional p‐Tyr protein spots were detected in pH 3–10 2‐DE images of butorphanol‐dependent rat brains. Immobilized pH strips with three different narrow pH ranges were examined to improve the resolution of p‐Tyr proteins in 2‐DE gels. Fifty‐three p‐Tyr protein spots were identified as known proteins involved in cell cytoskeleton, cell metabolism, and cell signaling. This proteomic approach can provide useful information for understanding the complex mechanism of butorphanol dependence in vivo. © 2004 Wiley‐Liss, Inc.
Attacks on Heartbeat-Based Security Using Remote Photoplethysmography

The time interval between consecutive heartbeats (interpulse interval, IPI) has previously been suggested for securing mobile-health solutions. This time interval is known to contain a degree of randomness, permitting the generation of a time- and person-specific identifier. It is commonly assumed that only devices trusted by a person can make physical contact with him/her, and that this physical contact allows each device to generate a similar identifier based on its own cardiac recordings. Under these conditions, the identifiers generated by different trusted devices can facilitate secure authentication. Recently, a wide range of techniques have been proposed for measuring heartbeats remotely, a prominent example of which is remote photoplethysmography (rPPG). These techniques may pose a significant threat to heartbeat-based security, as an adversary may pretend to be a trusted device by generating a similar identifier without physical contact, thus bypassing one of the core security conditions. In this paper, we assess the feasibility of such remote attacks using state-of-the-art rPPG methods. Our evaluation shows that rPPG has similar accuracy as contact PPG and, thus, forms a substantial threat to heartbeat-based-security systems that permit trusted devices to obtain their identifiers from contact PPG recordings. Conversely, rPPG cannot obtain an accurate representation of an identifier generated from electrical cardiac signals, making the latter invulnerable to state-of-the-art remote attacks.
The Bricks and Mortar of our Foundation for Faculty Development: Book-study within a self-study professional learning community

This article examines the experiences of seven teacher educators who met monthly over one academic year to engage in a collaborative self-study focused on exploring the text, Developing a Pedagogy of Teacher Education: Understanding Teaching and Learning about Teaching. Through reflective discussion focused on relating the text to our practices, we enhanced understandings of ourselves, one another, our students, and our teacher education program. Description and analysis are provided through two frameworks. First, we detail our engagement in authentic conversations about our tensions, documenting how we negotiated the same six tensions as teacher educators and in learning about teaching teachers through collaborative self-study. Next, we illustrate how exemplifying the key characteristics of effective professional learning community groups supported our collaborative exploration. We also detail how we moved increasingly beyond book-study to focus on our context-specific needs and co-construct possibilities for the future. Implications of considerations in forming a collaborative self-study group are identified.
Osteopontin expression in reactive lesions of gingiva

Reactive proliferations of the gingiva comprise lesions such as pyogenic granuloma (PG), inflammatory fibroepithelial hyperplasia (IFH), peripheral ossifying fibroma (POF), and peripheral giant cell lesion. Osteopontin (OPN) has a dual role, it promotes mineralization when it is bound to solid substrate, and on the other hand, it inhibits mineralization when it is seen in association with solution. Objectives The study aimed to evaluate the expression of osteopontin in normal gingival tissue and different types of focal reactive proliferations of gingival tissue, and its role in the development of calcification within it. Material and Methods The presence and distribution of osteopontin was assessed using immunohistochemistry in five cases of normal gingival tissue and 30 cases of focal reactive proliferations of gingiva. Results There was no expression of osteopontin in normal subjects. Few cases of pyogenic granuloma, inflammatory fibroepithelial hyperplasia, and all the cases of peripheral ossifying fibroma showed positivity for osteopontin in the inflammatory cells, stromal cells, extracellular matrix, and in the calcifications. Conclusion The expression of osteopontin in all the cases of peripheral ossifying fibroma speculates that the majority of the cases of peripheral ossifying fibroma originate from the periodontal ligament cells. The treatment modalities for peripheral ossifying fibroma should differ from other focal reactive proliferations of gingiva.
A review of health-related work outcome measures and their uses, and recommended measures.

Despite the growing recognition that work can contribute to the development of musculoskeletal disorders, there are almost no data on whether and how physicians investigate the contribution of work to patients’ health status or the influence of health status on work performance. This is particularly true of primary care, where much of the medical care for patients with work-related low back pain is provided. As more patients with musculoskeletal injuries show up in primary care settings, it will become important to document health-related work outcomes and incorporate into practice outcome tools that enable the physician to obtain a quick and accurate accounting of needed information about patients’ work. Health-related work outcomes relate to a person’s labor market status: Is a person working or not working? How well is he or she working? Did the person return to a job of pay and skill comparable to the preinjury job? Outcomes can incorporate time: How long has a person been out of work? How many hours, days, or weeks has a person been reported absent? Is the person working fullor part-time? How many hours does the person perform at full effectiveness? Finally, health-related work outcomes can capture the interplay between a person’s health status and work role performance: How difficult is it for a person with a given health status to perform work activities? Typically, health-related work outcomes have not specifically referred to unpaid work activities, such as volunteer work or household labor. The authors support the importance of capturing both paid and unpaid work outcomes, but in this article, paid work is the focus. Multiple publications in the literature contribute conceptually and methodologically to the health-related work outcomes field. These range from industrial psychology and labor economics to health services research, epidemiology, and pharmacoeconomics. In this paper, a window into health-related work outcomes research is created by considering the reasons for measuring these outcomes and briefly reviewing and illustrating several classes of measures. The advantages and limitations of each measure will be discussed, as the authors draw examples from own work. Although prior work has focused on upper extremity musculoskeletal disorders, the general principles for using health-related work outcomes are similar for researchers studying back injuries and disorders. In addition, a new work-related health outcome tool for measuring successful return to work (RTW) is discussed to illustrate a new class of measures. Hereafter, health-related work outcomes as are referred to as work outcomes.
Anti‐Vel 2, a New Antibody Showing Heterogeneity of Vel System Antibodies 1

Anti‐Vel 2 is a newly recognized antibody in the Vel system. Two anti‐Vel sera have been shown to contain anti‐Vel 2, while 4 others have anti‐Vel 1 plus anti‐Vel 2. The new phenotype Vel: 1, ‐2 is twice as common (frequency about 0.00053) as Vel: ‐1, ‐2 (frequency about 0.00025). Tests with the original Vel serum of Sussman and Milleh [8] show that the phenotype Vel: ‐1, ‐2 corresponds to the original Vel‐negative type.
Pharmacologic and Physiologic Influences Affecting Sensory Evoked Potentials: Implications for Perioperative Monitoring

EVOKED potentials (EPs) are the electrophysiologic responses of the nervous system to sensory or motor stimulation. 1,2 Stimulating the nervous system initiates the transmission of neural signals that may be recorded as EPs from various points along the stimulated pathway. Intraoperative monitoring (IOM) of EP has gained pop-ularity because EPs reﬂect the functional integrity of neural pathways in anesthetized patients undergoing surgical procedures that place nervous system structures in jeopardy. EPs monitored intraoperatively include somatosensory evoked potentials (SSEPs), brainstem auditory evoked potentials (BAEPs; also referred to as auditory brainstem responses), visual evoked potentials (VEPs)
SSH over SCTP — Optimizing a multi-channel protocol by adapting it to SCTP

Secure Shell (SSH) is a multi-channel security protocol running over the Transmission Control Protocol (TCP), which offers channels for several services over a secured connection, such as remote shells and connection forwarding. In this paper we introduce a method for using SSH over the Stream Control Transmission Protocol (SCTP), a transport protocol supporting multi-homing and multi-streaming. We examine benefits of this adaptation, which can be made available to generic applications with SSH's connection forwarding without further changes. The results also apply to other application protocols supporting a concept similar to channels.