Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of online group exercises for older adults on physical, psychological and social wellbeing: a randomized pilot trial

Background Intervention programs to promote physical activity in older adults, either in group or home settings, have shown equivalent health outcomes but different results when considering adherence. Group-based interventions seem to achieve higher participation in the long-term. However, there are many factors that can make of group exercises a challenging setting for older adults. A major one, due to the heterogeneity of this particular population, is the difference in the level of skills. In this paper we report on the physical, psychological and social wellbeing outcomes of a technology-based intervention that enable online group exercises in older adults with different levels of skills. Methods A total of 37 older adults between 65 and 87 years old followed a personalized exercise program based on the OTAGO program for fall prevention, for a period of eight weeks. Participants could join online group exercises using a tablet-based application. Participants were assigned either to the Control group, representing the traditional individual home-based training program, or the Social group, representing the online group exercising. Pre- and post- measurements were taken to analyze the physical, psychological and social wellbeing outcomes. Results After the eight-weeks training program there were improvements in both the Social and Control groups in terms of physical outcomes, given the high level of adherence of both groups. Considering the baseline measures, however, the results suggest that while in the Control group fitter individuals tended to adhere more to the training, this was not the case for the Social group, where the initial level had no effect on adherence. For psychological outcomes there were improvements on both groups, regardless of the application used. There was no significant difference between groups in social wellbeing outcomes, both groups seeing a decrease in loneliness despite the presence of social features in the Social group. However, online social interactions have shown to be correlated to the decrease in loneliness in the Social group. Conclusion The results indicate that technology-supported online group-exercising which conceals individual differences in physical skills is effective in motivating and enabling individuals who are less fit to train as much as fitter individuals. This not only indicates the feasibility of training together despite differences in physical skills but also suggests that online exercise might reduce the effect of skills on adherence in a social context. However, results from this pilot are limited to a small sample size and therefore are not conclusive. Longer term interventions with more participants are instead recommended to assess impacts on wellbeing and behavior change.
Data reduction by removal of lurkers in OSN

With the advent of internet, online social network is seen as playing a very important role in connecting people and a platform to share ideas. In the current scenario, given restriction on resources available for advertisement, the best place to sell one's product would definitely be these online social networks. The popularity of social network has influenced the computer researchers to ponder on the question on who are the people playing vital roles in information spread. This paper reviews the state of art work done previously in estimation of influence in Online Social Network(OSN) and proposes an innovative idea to improve the existing influence estimation algorithm in terms of search space and runtime.
Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins[C][W][OPEN]

Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis. To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum–associated degradation (ERAD). Many secretory proteins are N-glycosylated, and despite some recent progress, little is known about the mechanism that selects misfolded glycoproteins for degradation in plants. Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD. Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process. By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins. Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch. Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
Personal exposure to PM2.5 and biomarkers of DNA damage.

Ambient particulate air pollution assessed as outdoor concentrations of particulate matter < or = 2.5 microm in diameter (PM(2.5)) has been associated with an increased cancer risk. However, outdoor PM(2.5) concentrations may not be the best measure of the individual particle exposure that is a sum of many sources besides outdoor particle levels, e.g., environmental tobacco smoke and cooking. We measured personal PM(2.5) and black smoke exposure in 50 students four times over 1 year and analyzed for biomarkers of different types of DNA damages. Ambient PM(2.5) concentrations were also measured. Exposure was measured for 48 h, after which blood samples were collected and analyzed for DNA damage in lymphocytes in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG), strand breaks, endonuclease III- and fapyguanine glycosylase-sensitive sites, and polyaromatic hydrocarbon adducts. Twenty-four-h urine collections were analyzed for 8-oxodG and 1-hydroxypyrene. Personal PM(2.5) exposure was found to be a predictor of 8-oxodG in lymphocyte DNA with an 11% increase in 8-oxodG/10 microg/m(3) increase in personal PM(2.5) exposure (P = 0.007). No other associations between exposure markers and biomarkers could be distinguished. The genotype of glutathione S-transferase M1 (GSTM1), T1 (GSTT1), and P1 (GSTP1) and NADPH:quinone reductase was also determined, but there were no effects of genotype on DNA polyaromatic hydrocarbon adducts or oxidative damage. The results suggest that moderate exposure to concentrations of PM can induce oxidative DNA damage and that personal PM(2.5) exposure is more important in this aspect than is ambient PM(2.5) background concentration.
Multiple time step diffusive Langevin dynamics for proteins

We present an algorithm for simulating the long time scale dynamics of proteins and other macromolecules. Our method applies the concept of multiple time step integration to the diffusive Langevin equation, in which short time scale dynamics are replaced by friction and noise. The macromolecular force field is represented at atomic resolution. Slow motions are modeled by constrained Langevin dynamics with very large time steps, while faster degrees of freedom are kept in local thermal equilibrium. In the limit of a sufficiently large molecule, our algorithm is shown to reduce the CPU time required by two orders of magnitude. We test the algorithm on two systems, alanine dipeptide and bovine pancreatic trypsin inhibitor (BPTI), and find that it accurately calculates a variety of equilibrium and dynamical properties. In the case of BPTI, the CPU time required is reduced by nearly a factor of 60 compared to a conventional, unconstrained Langevin simulation using the same force field. Proteins 30:215–227, 1998. © 1998 Wiley‐Liss, Inc.
Production, characterization, and immunohistochemical application of monoclonal antibodies to glutaminase purified from rat brain

Monoclonal antibodies were produced against phosphate-activated glutaminase (EC 3.5.1.2) as a marker for glutamatergic neurons: The enzyme was purified 1000-fold from rat brain mitochondria with a recovery of 27%. Upon SDS-PAGE the purified enzyme showed a single band up to 1.7 micrograms after the silver staining at molecular weight 62,000. Two monoclonal antibodies (IgMs) were produced; these absorbed more than 90% of glutaminase activity in rat brain homogenate. In immunoblotting after PAGE of the homogenate, the antibodies recognized only 1 protein band at the same position as that of the purified enzyme. Thus, the antibodies are specific and sufficient markers for glutaminase. Many neuronal cells in the rat brain were labeled immunohistochemically with these antibodies, but non-neuronal elements such as glial cells and vessels were not. Intense labeling was consistently observed in putative glutamatergic neurons such as pyramidal cells of layers V and VI in the cerebral neocortex. Intense staining was also seen in possible mossy fiber endings in the granular layer of the cerebellar cortex and in neurons giving off mossy fibers such as those in the pontine nuclei, pontine tegmental reticular nucleus of Bechterew, lateral reticular nucleus of the medulla oblongata, and external cuneate nucleus.
Applications of the NTID speech recognition test (NSRT®)

Abstract Objective: This study evaluated the diagnostic capabilities of an adaptive speech recognition protocol (NSRT®) that can be self-administered in non-clinical venues by listeners using internet-based software. Design: All participants were given an audiological evaluation, including pure-tone testing, and responded to the NSRT administered in quiet and + 5 dB SNR listening conditions. The NSRT test materials are sentence-length utterances containing phonetic contrasts, primarily minimal pairs. Study sample: Subjects were 123 adults with normal hearing to moderately severe sensorineural hearing loss (mean age = 55 years, SD = 23). Results: Performance on the NSRT is strongly related to pure-tone thresholds. Linear regression analyses support the utility of the NSRT as a proxy for clinically-obtained hearing thresholds across the octave frequencies 0.5 to 8 kHz, primarily for individuals in the − 10 to 55 dB HL range. Other NSRT results are linked to analyses of phonetic errors and components of aural rehabilitation. Conclusions: Among its numerous results, the NSRT yields quantitative predictions of frequency-specific hearing thresholds, provides insight into the phonetic errors that affect speech understanding in adults who suffer from sensorineural hearing loss, primarily in the − 10 to 55 dB HL range, and has implications for the design of individualized auditory training programs.
Gender Comparisons of Anthropometric Characteristics of Young Sprint Swimmers

Zuniga, J, Housh, TJ, Mielke, M, Hendrix, CR, Camic, CL, Johnson, GO, Housh, DJ, and Schmidt, RJ. Gender comparisons of anthropometric characteristics of young sprint swimmers. J Strength Cond Res 25(1): 103-108, 2011-The purpose of this study was to compare the body composition, body build, and anthropometric characteristics of boy and girl sprint swimmers. Two groups (boys, n = 38 and girls, n = 31) of sprint swimmers (mean age ± SD = 11.03 ± 2.29 and 10.45 ± 2.29 years, respectively) volunteered for this study. The subjects were members of local swimming clubs who competed in sprint swimming events (≤200 m). Gender comparisons were made for age, body weight (BW), height (HT), fat-free weight (FFW), percent body fat (%fat), endomorphic rating, mesomorphic rating, ectomorphic rating, sum of 12 diameters, sum of 11 circumferences, biacromial diameter/biiliac diameter, and FFW/HT. The results of the independent t-tests indicated that the only mean differences between the boy and girl sprint swimmers were for % fat (boys = 9.40 ± 5.35% fat; girls = 12.73 ± 6.19% fat) and endomorphic rating (boys = 2.87 ± 0.96; girls = 4.29 ± 1.22). For the current age group of sprint swimmers the only gender differences were for measures associated with body fatness, and there were no differences for body build measures associated with musculoskeletal size, muscularity, skeletal size, total body mass, or body breadth dimensions. These findings suggest that the swimming performance for girls may be improved through training programs designed to reduce body fatness.
Crystal structure of the human alkaline sphingomyelinase provides insights into substrate recognition

Absorption of dietary sphingomyelin (SM) requires its initial degradation into ceramide, a process catalyzed by the intestinal enzyme alkaline sphingomyelinase (alk-SMase, NPP7, ENPP7). alk-SMase belongs to the nucleotide pyrophosphatase/phosphodiesterase (NPP) family, the members of which hydrolyze nucleoside phosphates, phospholipids, and other related molecules. NPP7 is the only paralog that can cleave SM, and its activity requires the presence of bile salts, a class of physiological anionic detergents. To elucidate the mechanism of substrate recognition, we determined the crystal structure of human alk-SMase in complex with phosphocholine, a reaction product. Although the overall fold and catalytic center are conserved relative to other NPPs, alk-SMase recognizes the choline moiety of its substrates via an NPP7-specific aromatic box composed of tyrosine residues. Mutational analysis and enzymatic activity assays identified features on the surface of the protein—a cationic patch and a unique hydrophobic loop—that are essential for accessing SM in bile salt micelles. These results shed new light on substrate specificity determinants within the NPP enzyme family.
Estimating the prevalence of negative attitudes towards people with disability: a comparison of direct questioning, projective questioning and randomised response

Purpose. Despite being susceptible to social desirability bias, attitudes towards people with disabilities are traditionally assessed via self-report. We investigated two methods presumably providing more valid prevalence estimates of sensitive attitudes than direct questioning (DQ). Most people projective questioning (MPPQ) attempts to reduce bias by asking interviewees to estimate the number of other people holding a sensitive attribute, rather than confirming or denying the attribute for themselves. The randomised-response technique (RRT) tries to reduce bias by assuring confidentiality through a random scrambling of the respondent's answers. Method. We assessed negative attitudes towards people with physical and mental disability via MPPQ, RRT and DQ to compare the resulting estimates. Results. The MPPQ estimates exceeded the DQ estimates. Employing a cheating-detection extension of the RRT, we determined the proportion of respondents disregarding the RRT instructions and computed an upper bound for the prevalence of negative attitudes. MPPQ estimates exceeded this upper bound and were thus shown to overestimate the prevalence. Furthermore, we found more negative attitudes towards people with mental disabilities than those with physical disabilities in all three questioning conditions. Conclusions. We recommend employing the cheating-detection variant of the RRT to gain additional insight in future studies on attitudes towards people with disabilities.
LC/MS analysis of stratum corneum lipids: ceramide profiling and discovery[S]

Ceramides (CERs) in the upper layer of the skin, the stratum corneum (SC), play a key role in the skin barrier function. In human SC, the literature currently reports 11 CER subclasses that have been identified. In this paper, a novel quick and robust LC/MS method is presented that allows the separation and analysis of all known human SC CER subclasses using only limited sample preparation. Besides all 11 known and identified subclasses, a 3D multi-mass chromatogram shows the presence of other lipid subclasses. Using LC/MS/MS with an ion trap (IT) system, a Fourier transform-ion cyclotron resonance system, and a triple quadrupole system, we were able to identify one of these lipid subclasses as a new CER subclass: the ester-linked ω-hydroxy fatty acid with a dihydrosphingosine base (CER [EOdS]). Besides the identification of a new CER subclass, this paper also describes the applicability and robustness of the developed LC/MS method by analyzing three (biological) SC samples: SC from human dermatomed skin, human SC obtained by tape stripping, and SC from full-thickness skin explants. All three biological samples showed all known CER subclasses and slight differences were observed in CER profile.
Heterogeneity of the Mitochondrial Proteome for Photosynthetic and Non-photosynthetic Arabidopsis Metabolism*S

Heterogeneity of the mitochondrial proteome in plants underlies fundamental differences in the roles of these organelles in different tissues. We quantitatively compared the mitochondrial proteome isolated from a non-photosynthetic cell culture model with more specialized mitochondria isolated from photosynthetic shoots. Differences in intact mitochondrial respiratory rates with various substrates and activities of specific enzymes provided a backdrop of the functional variation between these mitochondrial populations. Proteomics comparisons provided a deep insight into the different steady-state abundances of specific mitochondrial proteins. Combined these data showed the elevated level of the photorespiratory apparatus and its complex interplay with glycolate, cysteine, formate, and one-carbon metabolism as well as the decrease of selected parts of the tricarboxylic acid cycle, alterations in amino acid metabolism focused on 2-oxoglutarate generation, and degradation of branched chain amino acids. Comparisons with microarray analysis of these tissue types showed a positive, mild correlation between mRNA and mitochondrial protein abundance, a tighter correlation for specific biochemical pathways, but over 78% concordance in direction between changes in protein and transcript abundance in the two tissues. Overall these results indicated that the majority of the variation in the plant mitochondrial proteome occurred in the matrix, highlighted the constitutive nature of the respiratory apparatus, and showed the differences in substrate choice and/or availability during photosynthetic and non-photosynthetic metabolism.
Kinetic and Mutational Analyses of the Major Cytosolic Exopolyphosphatase from Saccharomyces cerevisiae*

Yeast exopolyphosphatase (scPPX) processively splits off the terminal phosphate group from linear polyphosphates longer than pyrophosphate. scPPX belongs to the DHH phosphoesterase superfamily and is evolutionarily close to the well characterized family II pyrophosphatase (PPase). Here, we used steady-state kinetic and binding measurements to elucidate the metal cofactor requirement for scPPX catalysis over the pH range 4.2-9.5. A single tight binding site for Mg2+ (Kd of 24 μm) was detected by equilibrium dialysis. Steady-state kinetic analysis of tripolyphosphate hydrolysis revealed a second site that binds Mg2+ in the millimolar range and modulates substrate binding. This step requires two protonated and two deprotonated enzyme groups with pKa values of 5.0–5.3 and 7.6–8.2, respectively. The catalytic step requiring two deprotonated groups (pKa of 4.6 and 5.6) is modulated by ionization of a third group (pKa of 8.7). Conservative mutations of Asp127, His148, His149 (conserved in scPPX and PPase), and Asn35 (His in PPase) reduced activity by a factor of 600–5000. N35H and D127E substitutions reduced the Mg2+ affinity of the tight binding site by 25–60-fold. Contrary to expectations, the N35H variant was unable to hydrolyze pyrophosphate, but markedly altered metal cofactor specificity, displaying higher catalytic activity with Co2+ bound to the weak binding site versus the Mg2+- or Mn2+-bound enzyme. These results provide an initial step toward understanding the dynamics of scPPX catalysis and reveal significant functional differences between structurally similar scPPX and family II PPase.
Basal Urinary Zinc/Creatinine Ratio as an Indicator of Dietary Zinc Intake in Healthy Adult Women

Objective: To study, in healthy women, the correlation between the basal urinary zinc/creatinine ratio and dietary zinc intake. Subjects: A group of 36 healthy female University students was evaluated. Mean age and body weight were, respectively, 25.6±3.3 years and 54.4±7.0 kg. Methods: Basal urine was collected; Zn was determined by AAS and Creatinine (Creat) by the Jaffe method. A nutritional survey of seven days was recorded. Mean daily dietary intake of energy (DE) and zinc (DZn) were calculated according to the INCAP and English or German Food Composition Tables, respectively. Results: Mean dietary daily intake were as follows (±SD): Energy (kcal): 1606±570; zinc (mg): 9.1±3.8; basal urine Zn/Creat ratio: 0.41±0.24. Individual values of the Zn/Creat ratio correlated with dietary Zn (r=0.481, p=0.0339); data grouped according to ranges of dietary Zn fit the following equation: Zn/Creat=0.160±0.034 DZn (mg/day); (r=0.870, p=0.00497). Conclusions: These results showed that the basal urinary Zn/Creat ratio could be a useful indicator of dietary Zn intake in healthy adult women.
The Putative Transmembrane Segment 7 of Human Organic Anion Transporter hOAT1 Dictates Transporter Substrate Binding and Stability

Human organic anion transporter hOAT1 plays a critical role in the body disposition of clinically important drugs. We examined the role of the putative transmembrane segment (TM) 7 in the function of hOAT1. Each residue within putative TM7 was replaced by alanine, and the uptake of para-aminohippurate was studied in cells expressing the mutants. We discovered four critical amino acid residues: Trp-346, Thr-349, Tyr-353, and Tyr-354. Substitution of Tyr-353 and Tyr-354 with alanine led to the loss of transport activity without affecting the surface expression of the transporter, whereas substitution of Trp-346 and Thr-349 with alanine lead to the loss of the total expression of the transporter. The effect of side chains of Tyr-353 and Tyr-354 on transporter functions were further evaluated by replacing these residues with Phe or Trp. Among all the mutants studied (Y353W, Y353F, Y354W, and Y354F), only mutant Y353F regained 30% transport activity, which was lost from replacement of Tyr-353 with alanine, suggesting that both the -OH group and the size of the side chain at positions 353 and 354 are critical for maintaining the full transport activity. To investigate the mechanisms underlying the loss of total protein expression when Trp-346 and Thr-349 were replaced with alanine, mutant-expressing cells were treated with lysosomal or proteasomal inhibitors. Our results showed that only proteasomal inhibitors resulted in the accumulation of mutant proteins, indicating that proteasome is involved in the degradation of the mutant transporters. Therefore, Trp-346 and Thr-349 are critically involved in the stability of the transporter.