Miracle-dz/UniFAR-FTUs huggingface dataset:
Gas injection may have triggered earthquakes in the Cogdell oil field, Texas

Significance Between 2006 and 2011 a series of earthquakes occurred in the Cogdell oil field near Snyder, TX. A previous series of earthquakes occurring 1975–1982 was attributed to the injection of water into wells to enhance oil production. We evaluated injection and extraction of oil, water, and gas in the Cogdell field. Water injection cannot explain the 2006–2011 earthquakes. However, since 2004 significant volumes of gas including CO2 have been injected into Cogdell wells. If this triggered the 2006–2011 seismicity, this represents an instance where gas injection has triggered earthquakes having magnitudes 3 and larger. Understanding when gas injection triggers earthquakes will help evaluate risks associated with large-scale carbon capture and storage as a strategy for managing climate change. Between 1957 and 1982, water flooding was conducted to improve petroleum production in the Cogdell oil field north of Snyder, TX, and a contemporary analysis concluded this induced earthquakes that occurred between 1975 and 1982. The National Earthquake Information Center detected no further activity between 1983 and 2005, but between 2006 and 2011 reported 18 earthquakes having magnitudes 3 and greater. To investigate these earthquakes, we analyzed data recorded by six temporary seismograph stations deployed by the USArray program, and identified 93 well-recorded earthquakes occurring between March 2009 and December 2010. Relocation with a double-difference method shows that most earthquakes occurred within several northeast–southwest-trending linear clusters, with trends corresponding to nodal planes of regional focal mechanisms, possibly indicating the presence of previously unidentified faults. We have evaluated data concerning injection and extraction of oil, water, and gas in the Cogdell field. Water injection cannot explain the 2006–2011 earthquakes, especially as net volumes (injection minus extraction) are significantly less than in the 1957–1982 period. However, since 2004 significant volumes of gases including supercritical CO2 have been injected into the Cogdell field. The timing of gas injection suggests it may have contributed to triggering the recent seismic activity. If so, this represents an instance where gas injection has triggered earthquakes having magnitudes 3 and larger. Further modeling studies may help evaluate recent assertions suggesting significant risks accompany large-scale carbon capture and storage as a strategy for managing climate change.
Gene expression profiling in uveal melanoma: two regions on 3p related to prognosis.

PURPOSE Although studies on uveal melanoma (UM) revealed prognostic significance of chromosomal aberrations, they resulted in classification errors in survival prediction. A robust prognostic classifier with strong predictive value and further insight in genes responsible for poor prognosis were obtained by performing a gene-expression profile in tumors of UM patients for which extensive clinical, histopathologic, cytogenetic, and follow-up data were available. Furthermore, the UM microarray expression data were compared with cytogenetic data. METHODS Gene-expression profiles of 46 UMs were obtained with microchip assays. Data were analyzed with cluster-analysis and predictive analysis of microarrays (PAM) software and validated with real-time PCR. The prognostic significance of UMs with specific molecular signatures was determined. Furthermore, LAP analysis resulted in the identification of differentially expressed chromosomal regions. RESULTS The primary UMs were classified in two distinct molecular classes with a strong prognostic value (P < 0.001; hazard ratio 7.7). Classifier gene sets for microarray class and disease-free survival were validated with real-time PCR, and the predictive value of the UM class marker set was validated with gene-expression profiles of tumors provided by other institutions, showing a sensitivity of 0.93 and specificity of 1.00 for class II tumors. A locally adaptive statistical procedure identified two regions on the short arm of chromosome 3 with decreased gene-expression in tumors with shorter disease-free survival. CONCLUSIONS Microarray classification outperforms known prognostic indicators for UM, such as clinical, histopathologic, and cytogenetic parameters. In addition, the identified regions with lower expressed genes on 3p could harbor genes that are responsible for the poor prognosis of patients with UM.
Reiterative use of the notch signal during zebrafish intrahepatic biliary development

The Notch signaling pathway regulates specification of zebrafish liver progenitor cells towards a biliary cell fate. Here, using staged administration of a pharmacological inhibitor of Notch receptor processing, we show that activation of the Notch pathway is also important for growth and expansion of the intrahepatic biliary network in zebrafish larvae. Biliary expansion is accompanied by extensive cell proliferation and active remodeling of the nascent ductal network, as revealed by time lapse imaging of living zebrafish larvae that express a Notch responsive fluorescent reporter transgene. Together, these data support a model in which the Notch signal functions reiteratively during biliary development; first to specific biliary cells and then to direct remodeling of the nascent biliary network. As the Notch pathway plays a comparable role during mammalian biliary development, including humans, these studies also indicate broad conservation of the molecular mechanisms directing biliary development in vertebrates. Developmental Dynamics 239:855–864, 2010. © 2010 Wiley‐Liss, Inc.
Variant treatment for gastric varices with polidocanol foam using balloon‐occluded retrograde transvenous obliteration: A pilot study

Treatment of gastric variceal rupture remains difficult with current options including transjugular intrahepatic portosystemic shunt and endoscopic therapies having significant side effects or reduced efficacy. We report five cases of gastric varices that were successfully treated with balloon‐occluded retrograde transvenous obliteration of varices (BRTO) using Polidocanol foam as an alternative sclerosant to ethanolamine oleate.
The role of implementation planning in increasing physical activity identification.

OBJECTIVE To shed light on the process of identification with physical activity. METHODS The study involved a prospective design and a sample of 111 university students. RESULTS Results revealed that (1) identifying with physical activity increased the likelihood that students formed implementation plans that, in turn, resulted in greater goal progress, and (2) planning for and progressing on a physical activity goal was associated to increased physical activity identification over time. CONCLUSION Implementation planning should be included in interventions aimed at promoting positive changes in physical activity identification and progress on physical activity goals.
Prefracture syndrome of the hip fracture: a case control study.

BACKGROUND Hip fractures result from both bone fragility and trauma, more often a sideways fall. Spontaneous hip fractures have been described; in such cases, patients reported pain ("prefracture" syndrome) in the hip region for weeks before the fracture. OBJECTIVES To identify the proportion of patients who had a pain in the hip region before a hip fracture, to compare this proportion to the one observed in controls and to describe the characteristics of this pain. PATIENTS AND METHODS For a period of 6 months, each subject (>65 years) treated for hip fracture was prospectively recruited in an orthopaedic surgery department. Exclusion criteria were: alterations of cognitive functions (defined by a mini mental state <20), refusal, and fractures related to bone metastasis or multiple myeloma. Subjects were compared to sex-matched controls consulting in an acute care geriatrics unit. They were asked about the occurrence of pain in hip region before the fracture and its characteristics. RESULTS Thirty-eight patients (31 women, 7 men, mean age 83.1 [+/-7.6]) were included and were compared to 38 sex-matched controls (31 women, 7 men, mean age 82.7 [+/-6.9]). Among the 38 patients with hip fracture, 10 (26.3%) reported a pain in the hip region, compared with 2 (5.3%) in the control group (p=0.01). CONCLUSION A better recognition of "prefracture" pain in the elderly may allow adequate management and treatment of patients, in order to avoid a proportion of hip fractures.
Loop Electrosurgical Excision Procedure and the Risk for Preterm Delivery

OBJECTIVE: To estimate whether the severity of cervical intraepithelial neoplasia (CIN) and the loop electrosurgical excision procedure (LEEP) increase the risk for preterm delivery, and to evaluate the role of repeat LEEP and time interval since LEEP. METHODS: This was a retrospective register-based study from Finland from 1997 to 2009. We linked Hospital Discharge Register and Finnish Medical Birth Register data. Case group women consisted of 20,011 women who underwent LEEP during the study period and their subsequent singleton deliveries in 1998–2009. Control population included women from the Medical Birth Register with no LEEP (n=430,975). The main outcome measure was preterm delivery before 37 weeks of gestation. RESULTS: The risk for preterm delivery increased after LEEP. Women with previous LEEP had 547 (7.2%) preterm deliveries, whereas the control population had 30,151 (4.6%) preterm deliveries (odds ratio [OR] 1.61, confidence interval [CI] 1.47–1.75, number needed to harm 38.5). The overall preterm delivery rate in the study period was 4.6% for singleton deliveries. Repeat LEEP was associated with an almost threefold risk for preterm delivery (OR 2.80, CI 2.28–3.44). The severity of CIN did not increase the risk for preterm delivery. However, with LEEP for carcinoma in situ or microinvasive cancer, the risk for preterm delivery was higher (OR 2.55, CI 1.68–3.87). The increased risk also was associated with non-CIN lesions (OR 2.04, CI 1.46–2.87). Similarly, the risk was increased after diagnostic LEEP (OR 1.39, 95% CI 1.16–1.67). Time interval since LEEP was not associated with preterm delivery. Adjusting for maternal age, parity, socioeconomic or marital status, urbanism, and previous preterm deliveries did not change the results. CONCLUSION: The risk for preterm delivery was increased after LEEP regardless of the histopathologic diagnosis. The risk was highest after repeat LEEP, which should be avoided, especially among women of reproductive age. LEVEL OF EVIDENCE: II
Elucidation of Substrate Specificity in the Cobalamin (Vitamin B12) Biosynthetic Methyltransferases

Ring contraction during cobalamin (vitamin B12) biosynthesis requires a seemingly futile methylation of the C20 position of the tetrapyrrole framework. Along the anaerobic route, this reaction is catalyzed by CbiL, which transfers a methyl group from S-adenosyl-l-methionine to cobalt factor II to generate cobalt factor III. CbiL belongs to the class III methyltransferases and displays similarity to other cobalamin biosynthetic methyltransferases that are responsible for the regiospecific methylation of a number of positions on the tetrapyrrole molecular canvas. In an attempt to understand how CbiL selectively methylates the C20 position, a detailed structure function analysis of the enzyme has been undertaken. In this paper, we demonstrate that the enzyme methylates the C20 position, that its preferred substrate is cobalt factor II, and that the metal ion does not undergo any oxidation change during the course of the reaction. The enzyme was crystallized, and its structure was determined by x-ray crystallography, revealing that the 26-kDa protein has a similar overall topology to other class III enzymes. This helped in the identification of some key amino acid residues (Asp104, Lys176, and Tyr220). Analysis of mutant variants of these groups has allowed us to suggest potential roles that these side chains may play in substrate binding and catalysis. EPR analysis of binary and ternary complexes indicate that the protein donates a fifth ligand to the cobalt ion via a gated mechanism to prevent transfer of the methyl group to water. The chemical logic underpinning the methylation is discussed.
Constitutive Mutations of the OccR Regulatory Protein Affect DNA Bending in Response to Metabolites Released from Plant Tumors*

OccR is a LysR-type transcriptional regulator ofAgrobacterium tumefaciens that positively regulates the octopine catabolism operon of the Ti plasmid and is also an autorepressor. Positive control of the occ genes occurs in response to octopine, a nutrient released from crown gall tumors. OccR binds to a site upstream of the occQ promoter in the presence and absence of octopine. Octopine causes prebound OccR to undergo a conformational change at the DNA binding site that causes changes in footprint length and DNA bending. To determine the roles of these conformational changes in transcriptional activation, we isolated 22 OccR mutants that were able to activate the occQpromoter in the absence of octopine. Thirteen of these mutants contained single amino acid substitutions, and nine contained two base pair changes resulting in two amino acid substitutions, which in most cases acted synergistically. These mutations spanned the entire length of the protein. Most of these mutant proteins in the absence of octopine displayed DNA binding and bending properties characteristic of transcriptionally active OccR-octopine complexes.
High-dose immunoembolization: survival benefit in patients with hepatic metastases from uveal melanoma.

PURPOSE To retrospectively evaluate prognostic factors for survival in patients with uveal melanoma who received chemoembolization (CE) with 1,3-bis (2-chloroethyl)-1-nitrosourea or immunoembolization (IE) with granulocyte-macrophage colony-stimulating factor (GM-CSF) for hepatic metastases. MATERIALS AND METHODS Fifty-three consecutive patients with uveal melanoma were treated by using CE or IE in clinical trials approved by the Institutional Review Board. Prognostic factors associated with overall survival (OS) and progression-free survival (PFS) in the liver and extrahepatic (systemic) organs were retrospectively evaluated. Covariates of age, sex, preexisting extrahepatic metastases, liver enzyme levels, tumor volume, radiologic response in hepatic metastases, and treatment type were analyzed. RESULTS Compared with CE, high-dose (>or=1500 microg of GM-CSF) IE resulted in significantly better OS (20.4 vs 9.8 months, P = .005) and systemic PFS (12.4 vs 4.8 months, P = .001) at univariate analysis. Overall, women outlived men (14.4 vs 9.8 months, P = .01). Patients who achieved regression of hepatic metastases after embolization lived much longer than did those who did not achieve regression (27.2 vs 9.9 months, P < .001). At multivariate analysis, prolonged OS was confirmed for women, patients who underwent high-dose IE, younger patients (age < 60 years), and patients with regression of hepatic metastases. Independent predictors of longer systemic PFS included high-dose IE, younger age, and regression of hepatic metastases. No covariate predicted liver PFS except for hepatic response. CONCLUSION Treatment with high-dose IE prolonged survival of patients with uveal melanoma who received embolization of hepatic metastases and possibly delayed progression of extrahepatic metastases.
Structural and Functional Analysis of the Surface Protein of Human Coronavirus OC43

Abstract The two surface glycoproteins S and HE of human coronavirus OC43 (HCV-OC43) were isolated from the viral membrane and purified. Only the S protein was able to agglutinate chicken erythrocytes, indicating that this viral protein is the major hemagglutinin of HCV-OC43. The receptor determinant recognized by this virus on the surface of erythrocytes is N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) which is also used by bovine coronavirus for attachment to cells. By analyzing erythrocytes containing different amounts of Neu5,9Ac2 in either of two linkage types, it was found that there are subtle differences in the affinity of both viruses for 9-O-acetylated sialic acid. Bovine coronavirus was more efficient in recognizing low amounts of Neu5,9Ac2 β2,3 linked to galactose, whereas HCV-OC43 was superior with respect to the β2,6 linkage. The gene coding for the S protein of HCV-OC43 was cloned and sequenced. A large open reading frame predicts a polypeptide of 150 kDa in the unglycosylated form. A protein of about 190 kDa is expected if the 20 potential glycosylation sites are used for attachment of N-linked oligosaccharide side chains. These predictions were confirmed by in vitro transcription and translation of the gene in the presence or absence of canine pancreatic microsomal membranes. A high degree of sequence homology was found between the S proteins of HCV-OC43 and bovine coronavirus. Structural and functional analyses of more strains should help to identify the location of the sialic acid-binding site.
PARENTAL MORBIDITY, CHILD WORK, AND HEALTH INSURANCE IN RWANDA

Abstract Measuring direct and indirect effects of extending health insurance coverage in developing countries is a key issue for health system development and for attaining universal health coverage. This paper investigates the role played by health insurance in the relationship between parental morbidity and child work decisions. We use a propensity score matching technique combined with hurdle models, using data from Rwanda. The results show that parental health shocks have a substantial influence on child work when households do not have health insurance. Depending on the gender of the sick parent, there is a substitution effect not only between the parent and the child on the labor market, but also between the time the child spends on different work activities. Altogether, results reveal that health insurance protects children against child work in the presence of parental health shocks.
Expression of hepatitis B virus X protein in HBV‐infected human livers and hepatocellular carcinomas

Transactivation of cellular genes and functional inactivation of p53 by the hepatitis B virus (HBV) X gene‐encoded protein (HBx) are proposed as alternative mechanisms for induction of hepatocellular carcinomas (HCCs) in chronic HBV infection. Using an immunohistochemical approach, we studied the expression of HBx in 39 explanted livers with HBV‐associated disease. Because the data reported previously have been inconsistent, possibly due to the application of different antibodies, we compared results with 5 polyclonal and 6 monoclonal anti‐HBx antibodies from five laboratories. Ten of the 11 antibodies reacted with recombinant HBx by Western blotting, but only 1 polyclonal and 2 monoclonal antibodies reacted specifically with HBx in tissue, and were thus suitable for immunohistochemistry. Three other polyclonal antibodies reacted with tissue components in addition to HBx. One polyclonal and 4 monoclonal antibodies did not recognize the HBx in the tissue. HBx was demonstrated in 16 of 30 (53.3%) cirrhotic livers and 10 of 18 (58.8%) HCCs by all specific antibodies. The expression of HBx, among three HBV antigens examined, was found to be preferentially maintained in HCC and the surrounding liver parenchyma, including focal or nodular preneoplastic lesions. However, the immunoreactivity was always limited to the cytoplasm of a small number of parenchymal and neoplastic cells. The role of X gene expression in HBV‐associated human hepatocarcinogenesis remains to be established.
Climatic Variables and Transmission of Malaria: A 12-Year Data Analysis in Shuchen County, China

Objective. The objective of this study was to explore the impact of climate variability on the transmission of malaria, a vector-borne disease, in a county of China and provide suggestions to similar regions for disease prevention. Methods. A time-series analysis was conducted using data on monthly climatic variables and monthly incidence of malaria in Shuchen County, China, for the period 1980–1991. Results. Spearman's correlation analysis showed that monthly mean maximum and minimum temperatures, two measures of monthly mean relative humidity, and monthly amount of precipitation were positively correlated with the monthly incidence of malaria in the county. Regression analysis suggested that monthly mean minimum temperature and total monthly rainfall, with a one-month lagged effect, were significant climatic variables in the transmission of malaria in Shuchen County. Seaonality was also significant in the regression model and there was a declining secular trend in the incidence of malaria. Conclusion. The results indicate that climatic variables should be considered as possible predictors for regions with similar geographic, climatic, and socioeconomic conditions to those of Shuchen County.
A novel approach for flexible automation production systems

Nowadays, the constantly changing market demands make modern automation systems offer more strict flexibility and efficiency requirements as energy efficiency, performance optimization and tolerance to process or controllers faults… The introduction of reconfiguration mechanisms within production automation systems allow meeting some of these requirements but, at the same time, they lead more complex design and implementation of the automation system. This paper explores the advantages of Model Driven Engineering techniques to manage such complexity. The proposed approach is based on two well spread standards in the automation field: AutomationML and IEC 61131-3 which have a Markup Language notation (AML and PLCopen XML respectively).
Monaural envelope correlation perception in listeners with normal hearing and cochlear impairment.

Monaural envelope correlation perception was investigated in listeners with normal hearing and in listeners with cochlear hearing loss. Using a three-interval forced-choice procedure, the task of the subject was to identify the one interval out of three where the noise bands had correlated envelopes. Performance was determined as a function of the spectral separation between noise bands (delta f of 250, 500, or 1000 Hz) and the number of noise bands present (two, three, or five). Although individual differences existed, the results generally indicated better performance for the listeners with normal hearing when the delta f between bands was relatively small; however, there was no significant effect of hearing loss when the frequency separation between bands was greater than 250 Hz. The listeners with normal hearing generally showed decreased performance with increasing delta f, whereas the performance of many of the listeners with hearing impairment usually did not change appreciably with variation in delta f. Both groups of listeners showed improved performance with increasing number of noise bands present for the 500-Hz delta f. Only the listeners with hearing impairment showed significantly improved performance with increasing band number for the 250-Hz delta f; neither group showed improved performance with increasing band number for the 1000-Hz delta f. With five bands present, the performance of the listeners with hearing impairment did not differ significantly from that of the listeners with normal hearing, even for the 250-Hz delta f. (ABSTRACT TRUNCATED AT 250 WORDS)