Miracle-dz/UniFAR-FTUs huggingface dataset:
Involvement of the drosophila taurine/aspartate transporter dEAAT2 in selective olfactory and gustatory perceptions

Excitatory amino acid transporters (EAATs) are membrane proteins involved in the uptake of neurotransmitter amino acids in the nervous system. The Drosophila dEAAT2 gene was previously described to encode a taurine/aspartate transporter. To analyze further the expression pattern and physiological function of this protein, we generated transgenic flies containing either the dEAAT2 promoter region fused to GAL4 (dEAAT2‐GAL4) or a transgene allowing expression of a dEAAT2::GFP fusion protein (UAS‐dEAAT2::GFP). We observed that dEAAT2‐GAL4 expresses green fluorescent protein (GFP) in neurons in central and peripheral structures of third‐instar larvae and adult flies. Labeled neurons were found in olfactory and gustatory pathways, in which dEAAT2::GFP was detected from the dendrites of the sensory neurons up to the first‐ and second‐order centers. dEAAT2‐GAL4 is also expressed in mechanosensory neurons. We found that a viable piggyBac insertion strain disrupts dEAAT2 expression. This mutant appears morphologically normal and presents no locomotor or phototaxis impairments; however, its brain taurine level is significantly reduced compared with that of wild‐type flies. The dEAAT2 mutant showed decreased avoidance behavior in the presence of high concentration of propionic acid compared with wild‐type flies, but no modification of the avoidance response to benzaldehyde. In gustatory tests, both mutant and control flies were normally attracted to sucrose; however, the dEAAT2 mutant presented a higher salt sensitivity, being repulsed by low and high salt concentrations. Therefore, we conclude that dEAAT2 does function as a taurine transporter in vivo and that this protein is physiologically required for the sensory perception of specific environmental molecules. J. Comp. Neurol. 519:2734–2757, 2011. © 2011 Wiley‐Liss, Inc.
Simulation on the overvoltage of 500 kV fault current limiter based on fault current capture technology

With the development of 500 kV power system, the fault current capacity has been increased rapidly. A 500 kV fault current limiter model was founded based on fault current capture technology and vacuum switch technology. The fault current limiter had two operation modes. One was named FFCL (Forced zero-crossing Fault Current Limiter) in which the current was directly converted to the limiting reactor with a forced current zero-crossing, and the other was named IFCL (Inherent zero-crossing Fault Current Limiter) in which the current was converted to the limiting reactor with a inherent current zero-crossing. The operating overvoltage was simulated under different arrangement of RC absorber and arrester in FFCL and IFCL. The simulation result showed that the operating overvoltage was not remarkable in IFCL and extreme huge in FFCL. Finally, an optimum structure was put forward for the 500 kV fault current limiter.
Echocardiographic abnormalities with acute cardiac allograft rejection in children: correlation with endomyocardial biopsy.

In patients who have undergone successful orthotopic heart transplantation, echocardiography has the potential to be a noninvasive method for rejection surveillance that would reduce the frequency and guide the timing of endomyocardial biopsies, as well as allow for more frequent monitoring, with less cost and risk to the patient. To determine the applicability of echocardiography to detect moderate to severe rejection in children, 26 two-dimensional-guided M-mode echocardiograms with Doppler/color flow mapping were performed within 24 hours of endomyocardial biopsy. M-mode echocardiograms of the left ventricle were digitized and analyzed with a computer-assisted measurement format for left ventricular size, mass, and wall motion in systole and diastole. These echocardiographic parameters were clustered and analyzed by a unique echocardiographic scoring algorithm blinded to the biopsy interpretation. In eight cases in which findings of biopsies were consistent with moderate to severe rejection, left ventricular mass was increased and indexes of systolic and diastolic function were depressed compared with the remainder of the cases (n = 18), in which findings of biopsies included either no evidence or mild evidence of rejection. The echocardiographic score of the group with moderate to severe rejection was significantly greater than the score of the group that was normal or had mild rejection (5.4 +/- 0.7 vs 2.2 +/- 0.3; p < 0.001). With rejection prospectively defined as an echocardiographic score of greater than or equal to score 4, echocardiography achieved 88% sensitivity and 83% specificity in detecting moderate to severe rejection. (ABSTRACT TRUNCATED AT 250 WORDS)
Tunable room temperature solid state THz source based on Smith-Purcell radiation

This paper presents the theory and design of a novel, low-power, compact, room-temperature, tunable, solid state THz source of Smith-Purcell radiation. The design comprises a Transferred Electron Device and a metal transmission grating separated by a high-K dielectric. When a sufficiently high electric field is applied across the cathode and anode of the device, space-charge regions (also known as dipole or Gunn domains) form within the device and propagate across it. The moving dipole domains couple to the grating electromagnetic modes thereby mimicking the bunching of electrons seen in free-electron lasers. The result is the generation of coherent Smith-Purcell radiation that is tunable via adjusting the voltage applied across the device. The design is scalable from RF, through the THz, into the long-wave infrared via grating geometry and device material changes. Time-domain full-wave electromagnetic simulations were performed to design such a device for use in the THz region. The simulation results predict coherent emission at 1.0 THz.
TinyNET: a tiny network framework for TinyOS

In this paper we present TinyNET, a modular framework that allows development and quick integration of protocols and applications for Wireless Sensor Networks in TinyOS. As a sample experience of software development using TinyNET, we employ an environmental monitoring application. We organize our network testbed in a converge-casting topology, where the sink gathers the data collected by all sensors. Routing toward the sink is achieved based on a hop-count based algorithm. Our framework also integrates support for the 6LowPAN standard, which becomes therefore integrated with other network components and allows, e.g., to query single sensors within the network using ping messages. Thanks to TinyNET, these messages will make transparent use of the underlying network protocols. The main advantages yielded by TinyNET are found in the modularity of network components, in specific code that transparently manages the interfaces among different modules and translates standard TinyOS interfaces into new TinyNET ones, and in the readily available infrastructure to multiplex different applications over the same network stack. This allows a global vision of the system as well as the chance to focus on the design of separate components.
Extraction et exploitation d'annotations sémantiques contextuelles à partir de texte

Cette these rentre dans le cadre du projet europeen SevenPro (Environnement d’ingenierie virtuel semantique pour la conception des produits) dont le but est d'ameliorer le processus d'ingenierie de production dans les entreprises de fabrication, au moyen de l’acquisition, de la formalisation et de l'exploitation des connaissances. Nous proposons une approche methodologique et logicielle pour generer des annotations semantiques contextuelles a partir de texte. Notre approche est basee sur des ontologies et sur les technologies du Web semantique. Dans une premiere partie, nous proposons une modelisation de la notion de « contexte » pour le texte. Cette modelisation peut etre percue comme une projection des differents aspects du «contexte» abordes par ses definitions dans la litterature. Nous proposons egalement une modelisation des annotations semantiques contextuelles, avec la definition des differents types de relations contextuelles pouvant exister dans le texte. Ensuite, nous proposons une methodologie generique pour la generation d’annotations semantiques contextuelles basees sur une ontologie du domaine qui exploite au mieux les connaissances contenues dans les textes. L’originalite de la methodologie est qu’elle utilise des techniques de traitement automatique de la langue ainsi que des grammaires d’extraction (engendrees automatiquement) de relations de domaine, de concepts et de valeurs de propriete afin de produire des annotations semantiques reliees avec des relations contextuelles. De plus, nous prenons en compte le contexte d’apparition des annotations semantiques pendant leur generation. Un systeme supportant cette methodologie a ete implemente et evalue.
RPCA-Based Noise Suppression in MEG Measurement for Improving Bio-Electromagnetic Source Estimation

Magnetoencephalography (MEG) is a promising technology, which could be used in a variety of biomedical applications. However, MEG electromagnetic measurement is usually degraded by noise. Noise suppression in MEG measurement is particularly challenging because it is difficult to remove the noise and preserve the information components in the MEG data. In this study, a novel noise suppression method, based on robust principal component analysis (RPCA) technique, is presented and applied to the estimation of bio-electromagnetic field in source space for the first time. The proposed method gives a constrained optimization of MEG electromagnetic domain transformations such that the matrix of transformed MEG measurement can be decomposed as the sum of a sparse matrix of noise and a low-rank matrix of denoised data. Applying the proposed method to a number of simulations showed significant improvement of the result accuracy.
Inhibitory effect of Chinese green tea on cigarette smoke-induced up-regulation of airway neutrophil elastase and matrix metalloproteinase-12 via antioxidant activity

Abstract Our recent study has indicated that Chinese green tea (Lung Chen), in which epigallocatechin-3-gallate (EGCG) accounts for 60% of catechins, protected cigarette smoke-induced lung injury. We now hypothesized that Lung Chen tea may also have potential effect on lung oxidative stress and proteases/anti-proteases in a smoking rat model. Sprague–Dawley rats were exposed to either sham air (SA) or 4% cigarette smoke (CS) plus 2% Lung Chen tea or water by oral gavage. Serine proteases, matrix metalloproteinases (MMPs) and their respective endogenous inhibitors were determined in bronchoalveolar lavage (BAL) and lung tissues by gelatin/casein zymography and biochemical assays. Green tea consumption significantly decreased CS-induced elevation of lung lipid peroxidation marker, malondialdehyde (MDA), and CS-induced up-regulation of neutrophil elastase (NE) concentration and activity along with that of α1-antitrypsin (α1-AT) and secretory leukoproteinase inhibitor (SLPI) in BAL and lung. In parallel, significant elevation of MMP-12 activity was found in BAL and lung of the CS-exposed group, which returned to the levels of SA-exposed group after green tea consumption but not CS-induced reduction of tissue inhibitor of metalloproteinase (TIMP)-1 activity, which was not reversed by green tea consumption. Taken together, our data supported the presence of local oxidative stress and protease/anti-protease imbalance in the airways after CS exposure, which might be alleviated by green tea consumption through its biological antioxidant activity.
Suramin analogues as subtype-selective G protein inhibitors.

G protein alpha subunits expose specific binding sites that allow for the sequential, conformation-dependent binding of protein reaction partners, e.g., G protein beta gamma dimers, receptors, and effectors. These domains represent potential sites for binding of low-molecular-weight inhibitors. We tested the following suramin analogues as G protein antagonists: 8-(3-nitrobenzamido)-1,3,5-naphtalenetrisulfonic acid (NF007), 8-(3-(3-nitrobenzamido)benzamido)-1,3,5-naphtalenetrisulfonic++ + acid NF018), 8,8'-(carbonylbis(imino-3,1-phenylene))bis-(1,3,5-naphtalenetri sulfonic acid) (NF023), 8,8'-(carbonylbis(imino-3,1-phenylene)carbonylimino-(3,1-phe nylene))bis-(1,3, 5-naphtalenetrisulfonic acid) (NF037), and suramin. The compounds suppressed [35S]GTPgammaS binding to purified, recombinant G protein alpha subunits, an effect that is due to inhibition of GDP release. Suramin is selective for recombinant Gsalpha-s (EC50 values o f approximately 240 nM; rank order of potency, suramin > NF037 > NF023 > NF018 > NF007), whereas NF023 is selective for recombinant Gi alpha-1 and recombinant Go alpha (EC50 value of approximately 300 nM; rank order of potency, NF023 > / = NF037 > suramin >0 NF018 > NF007). Selectivity was also demonstrated on a cellular level. In rat sympathetic neurons, alpha-2-adrenergic and muscarinic receptor-dependent inhibition of the voltage-sensitive calcium current is mediated by Gi/Go, whereas inhibition by vasoactive intestinal peptide (VIP) is mediated by Gs. Calcium current inhibition by alpha2-adrenergic and muscarinic receptors was greatly reduced when 100 microM NF023 was applied intracellularly, whereas the response to VIP was unaffected; in contrast, the response to VIP was blunted only with 100 microM suramin in the recording pipette. The suramin analogues do not interfere with the interaction between alpha subunits and G protein beta gamma dimer but compete with binding of the effector. The addition of purified adenylyl cyclase reverses the inhibitory effect of suramin on the rate of [35S]GTPgammaS binding to recombinant Gsalpha-s, indicating direct competition for a common site; similarly, immunoprecipitation by an antibody directed against an epitope of the effector binding site is inhibited by suramin. Our results show that it is possible to design G protein inhibitors that target the effector binding site on the alpha subunits.
Régression sur variable fonctionnelle : estimation, tests de structure et applications

Au cours des dernieres annees, la branche de la statistique consacree a l'etude de variables fonctionnelles a connu un reel essor tant en terme de developpements theoriques que de diversification des domaines d'application. Nous nous interessons plus particulierement dans ce memoire a des modeles de regression dans lesquels la variable reponse est reelle tandis que la variable explicative est fonctionnelle, c'est a dire a valeurs dans un espace de dimension infinie. Les resultats que nous enoncons sont lies aux proprietes asymptotiques de l'estimateur a noyau generalise au cas d'une variable explicative fonctionnelle. Nous supposons pour commencer que l'echantillon que nous etudions est constitue de variables α-melangeantes et que le modele de regression est de nature nonparametrique. Nous etablissons la normalite asymptotique de notre estimateur et donnons l'expression explicite des termes asymptotiquement dominants du biais et de la variance. Une consequence directe de ce resultat est la construction d'intervalles de confiance asymptotiques ponctuels dont nous etudions les proprietes aux travers de simulations et que nous appliquons sur des donnees liees a l'etude du courant marin El Nino. On etablit egalement a partir du resultat de normalite asymptotique et d'un resultat d'uniforme integrabilite l'expression explicite des termes asymptotiquement dominants des moments centres et des erreurs Lp de notre estimateur. Nous considerons ensuite le probleme des tests de structure en regression sur variable fonctionnelle et supposons maintenant que l'echantillon est compose de variables independantes. Nous construisons une statistique de test basee sur la comparaison de l'estimateur a noyau et d'un estimateur plus particulier dependant de l'hypothese nulle a tester. Nous obtenons la normalite asymptotique de notre statistique de test sous l'hypothese nulle ainsi que sa divergence sous l'alternative. Les conditions generales sous lesquelles notre resultat est etabli permettent l'utilisation de notre statistique pour construire des tests de structure innovants permettant de tester si l'operateur de regression est de forme lineaire, a indice simple, . Differentes procedures de reechantillonnage sont proposees et comparees au travers de diverses simulations. Nos methodes sont enfin appliquees dans le cadre de tests de non effet a deux jeux de donnees spectrometriques.
Design and Implementation of Municipal Services for Human Welfare By Using Smart Phones

In day to day life municipal services are provided by visiting municipal offices physically or through visiting their web sites. Various facilities such as e-banking, e-commerce, -e-booking etc. are provided by using mobile application. Use of mobile applications for distinct purpose is growing rapidly. People prefer to use mobile applications to carry out various daily activities. Hence there is a need to develop a mobile application which will help to provide municipal corporation services. The previous system used to serve citizens was based on website which was not so convenient. In this paper we have developed a new system i.e. Mobile Applications which is more convenient and efficient than the previous system. This paper presents new and efficient method to provide various municipal services through mobile application that are water ,drainage ,cleanliness ,street lightening which turns to be efficient in terms of time, cost and paper work.
Methionine Sulfoximine Treatment and Carbon Starvation Elicit Snf1-independent Phosphorylation of the Transcription Activator Gln3 in Saccharomyces cerevisiae*

Tor proteins are global regulators situated at the top of a signal transduction pathway conserved from yeast to humans. Specific inhibition of the two Saccharomyces cerevisiae Tor proteins by rapamycin alters many cellular processes and the expression of hundreds of genes. Among the regulated genes are those whose expression is activated by the GATA family transcription activator, Gln3. The extent of Gln3 phosphorylation has been thought to determine its intracellular localization, with phosphorylated and dephosphorylated forms accumulating in the cytoplasm and nucleus, respectively. Data presented here demonstrate that rapamycin and the glutamine synthetase inhibitor, methionine sulfoximine (MSX), although eliciting the same outcomes with respect to Gln3-Myc13 nuclear accumulation and nitrogen catabolite repression-sensitive transcription, generate diametrically opposite effects on Gln3-Myc13 phosphorylation. MSX increases Gln3-Myc13 phosphorylation and rapamycin decreases it. Gln3-Myc13 phosphorylation levels are regulated by at least three mechanisms as follows: (i) depends on Snf1 kinase as observed during carbon starvation, (ii) is Snf1-independent as observed during both carbon starvation and MSX treatment, and (iii) is rapamycin-induced dephosphorylation. MSX and rapamycin act additively on Gln3-Myc13 phosphorylation, but MSX clearly predominates. These results suggest that MSX- and rapamycin-inhibited proteins are more likely to function in separate regulatory pathways than they are to function tandemly in a single pathway as thought previously. Furthermore, as we and others have detected thus far, Gln3 phosphorylation/dephosphorylation is not a demonstrably required step in achieving Gln3 nuclear localization and nitrogen catabolite repression-sensitive transcription in response to MSX or rapamycin treatment.
Implementasi Manajemen Kurikulum Dalam Meningkatkan Mutu Pendidikan

The aim of this research is to know (1) the curriculum management model in Vocational High School of Migas Cepu. (2) Quality education development in Vocational High School of Migas Cepu. (3) The supporting and a prohibitive factors in implementation of curriculum management and quality development in Vocational High School of Migas Cepu. (4) The curriculum management role in quality education development. This research is a field research by descriptive qualitative method. In collecting data, writer apply the field observation, deep interview to the related interviewees and another related resource. The research outcome is (1) the Vocational High School of Migas Cepu is a recommended school by government to apply the current curriculum of 2013, therefore the Vocational High School of Migas Cepu is combining the curriculum of KTSP and curriculum of 2013. (2) Planning, developing, applying, and evaluating curriculum held comprehensively according to curriculum’s demand. (3) The Vocational High School of Migas Cepu, pay their attention to developing quality of education, this is evidenced by the outcome target presence in national test that reach in 100% in last four years. The curriculum management control a 90% of school quality development, this is evidenced by applying many innovations and changing of learning model in school.
Rescue of Fertility in Homozygous Mice for the Urokinase Plasminogen Activator Transgene by the Transplantation of Mouse Hepatocytes

Development of the urokinase plasminogen activator/SCID (uPA/SCID) transgenic mouse model has opened new perspectives for the study of different biological mechanisms such as liver regeneration, stem cell differentiation, and human hepatic pathogens. We observed that homozygous uPA/SCID mice (uPA+/+/SCID) had a small offspring, indicating a fertility defect. The goal of this study was thus to rescue the fertility of homozygous uPA mice. A deregulation of ovarian function with an absence of corpus luteum was observed in female uPA+/+/SCID mice. In male uPA+/+/SCID mice, a decrease of the weight of the testes, epididymis, seminal vesicle, and prostate was measured. This was associated with an absence of seminal and prostatic secretions and a reduction in testicular sperm production. We hypothesized that the infertility of mice was the consequence of uPA-induced liver injury. Thus, in order to rescue liver function, hepatocytes from mice negative for the uPA transgene were transplanted into uPA+/+/SCID mice. Thirty days after cell transplantation, the livers of transplanted uPA+/+/SCID mice were totally repopulated and presented a normal morphology. Furthermore, transplantation restored normal body weight, life span, and reproductive organ function. In conclusion, we demonstrated that the transplantation of uPA+/+/SCID mice with healthy hepatocytes was sufficient to rescue the reproductive capacity of female and male uPA homozygous animals, highlighting the importance of normal liver function to reproductive capability.
CUSTOMER’S PERCEPTION ON SERVICE QUALITY IN BANKS. - A CASE OF BHUTAN

The purpose of this paper is to evaluate the service quality in retail banking in the least developed countries like Bhutan . This is an analytical study based mainly on the primary data collected through structured questionnaire. The questionnaire has been personally administered on a sample size of 262, chosen from four banks in Bhutan in Central district of Thimphu (Thimphu City) and southern District of Chukha (Pheounsoling). The result indicates that customers' perception has reached on highest in the Prompt and accuracy in transactions of the bank and lowest in the service of modern equipment and decor. Due to the increasing competition in retail banking, customer service is a crucial part of Banks integrated strategy and bank must give their due attention to the customers' perception about service quality. The researcher is studying the relationship between service quality and customer satisfaction.