Miracle-dz/UniFAR-FTUs huggingface dataset:
Differences and Similarities between Allergic and Nonallergic Rhinitis in a Large Sample of Adult Patients with Rhinitis Symptoms

Background: Allergic rhinitis (AR) and nonallergic rhinitis (NAR) may present with different clinical and laboratory characteristics. Methods: A total of 1,511 consecutive patients, aged 18–81 years, diagnosed with rhinitis, 56% females and 44% males, underwent complete allergic evaluation including skin prick test, blood eosinophil counts, nasal eosinophil counts, peak nasal inspiratory flow (PNIF) measurement and evaluation of nasal symptoms using a visual analog scale (VAS). Results: A total of 1,107 patients (73%)had AR, whereas 404 (27%) had NAR. Patients with NAR were older and predominantly female. A higher nasal eosinophils count was associated with AR and a lack of clinical response to antihistamines. AR patients had more sneezing and nasal pruritus, whereas NAR was characterized mainly by nasal obstruction and rhinorrhea. AR patients had more severe symptoms and recurrent conjunctivitis, whereas NAR patients had slightly more frequent episodes of recurring headaches as well as olfactory dysfunction. PNIF, blood eosinophil counts and VAS of nasal symptoms were higher in patients with AR. In a final logistic regression model, 10 variables were statistically different between AR and NAR: age [OR 0.97 (95% CI 0.96–0.98)], sneezing [OR 4.09 (95% CI 2.78–6.00)], nasal pruritus [OR 3.84 (95% CI 2.60–5.67)], mild symptoms [OR 0.21 (95% CI 0.09–0.49)], intermittent/severe nasal symptoms [OR 3.66 (95% CI 2.06–6.50)], VAS [OR 1.06 (95% CI 1.04–1.08)], clinical response to antihistamines [OR 22.59 (95% CI 13.79–37.00)], conjunctivitis [OR 4.49 (95% CI 2.86–7.05)], PNIF [OR 1.01 (95% CI 1.00–1.01)] and nasal eosinophil counts [OR 1.14 (95% CI 1.10–1.18)]. Receiver operating characteristic analysis showed high predictive accuracy for a model including these variables independently of the diagnosis of AR/NAR (cutoff <0.74). Conclusions: We showed that the several clinical and laboratory parameters reported above may help to reinforce or exclude the diagnosis of AR obtained with skin prick test.
Prolonged local expression of anti‐CD4 antibody by adenovirally transduced allografts can promote long‐term graft survival

Currently, successful transplantation of allografts requires the systemic use of immunosuppressive drugs. These can cause serious morbidity due to toxicity and increased susceptibility to cancer and infections. Local production of immunosuppressive molecules limited to the graft site would reduce the need for conventional, generalized immunosuppressive therapies and thus educe fewer side effects. This is particularly salient in a disease like type 1 diabetes, which is not immediately life‐threatening yet islet allografts can effect a cure.
Design and characterization of a laterally mounted phased-array transducer breast-specific MRgHIFU device with integrated 11-channel receiver array.

PURPOSE This work presents the design and preliminary evaluation of a new laterally mounted phased-array MRI-guided high-intensity focused ultrasound (MRgHIFU) system with an integrated 11-channel phased-array radio frequency (RF) coil intended for breast cancer treatment. The design goals for the system included the ability to treat the majority of tumor locations, to increase the MR image's signal-to-noise ratio (SNR) throughout the treatment volume and to provide adequate comfort for the patient. METHODS In order to treat the majority of the breast volume, the device was designed such that the treated breast is suspended in a 17-cm diameter treatment cylinder. A laterally shooting 1-MHz, 256-element phased-array ultrasound transducer with flexible positioning is mounted outside the treatment cylinder. This configuration achieves a reduced water volume to minimize RF coil loading effects, to position the coils closer to the breast for increased signal sensitivity, and to reduce the MR image noise associated with using water as the coupling fluid. This design uses an 11-channel phased-array RF coil that is placed on the outer surface of the cylinder surrounding the breast. Mechanical positioning of the transducer and electronic steering of the focal spot enable placement of the ultrasound focus at arbitrary locations throughout the suspended breast. The treatment platform allows the patient to lie prone in a face-down position. The system was tested for comfort with 18 normal volunteers and SNR capabilities in one normal volunteer and for heating accuracy and stability in homogeneous phantom and inhomogeneous ex vivo porcine tissue. RESULTS There was a 61% increase in mean relative SNR achieved in a homogeneous phantom using the 11-channel RF coil when compared to using only a single-loop coil around the chest wall. The repeatability of the system's energy delivery in a single location was excellent, with less than 3% variability between repeated temperature measurements at the same location. The execution of a continuously sonicated, predefined 48-point, 8-min trajectory path resulted in an ablation volume of 8.17 cm(3), with one standard deviation of 0.35 cm(3) between inhomogeneous ex vivo tissue samples. Comfort testing resulted in negligible side effects for all volunteers. CONCLUSIONS The initial results suggest that this new device will potentially be suitable for MRgHIFU treatment in a wide range of breast sizes and tumor locations.
A human monoclonal antibody specific to placental alkaline phosphatase, a marker of ovarian cancer

Placental alkaline phosphatase (PLAP) is a promising ovarian cancer biomarker. Here, we describe the isolation, affinity-maturation and characterization of two fully human monoclonal antibodies (termed B10 and D9) able to bind to human PLAP with a dissociation constant (Kd) of 10 and 30 nM, respectively. The ability of B10 and D9 antibodies to recognize the native antigen was confirmed by Biacore analysis, FACS and immunofluorescence studies using ovarian cancer cell lines and freshly-frozen human tissues. A quantitative biodistribution study in nude mice revealed that the B10 antibody preferentially localizes to A431 tumors, following intravenous administration. Anti-PLAP antibodies may serve as a modular building blocks for the development of targeted therapeutic products, armed with cytotoxic drugs, radionuclides or cytokines as payloads.
Segregation of the classical transmitters norepinephrine and acetylcholine and the neuropeptide Y in sympathetic neurons: Modulation by ciliary neurotrophic factor or prolonged growth in culture

Recent evidence has demonstrated that cotransmission from mammalian neurons is not uniquely achieved by costorage and corelease of transmitters and cotransmitters from single varicosities, but also by the concurrent release of mediators segregated in separate synapses of individual neurons. An important question to be addressed is whether neurons show defined patterns of segregation or whether this is a plastic feature. We addressed this question by exploring the segregation pattern of the classical sympathetic transmitters norepinephrine (NE) and acetylcholine (ACh) and the cotransmitter neuropeptide Y (NPY) in sympathetic ganglionic neurons cocultured with cardiac myocytes. Using antibodies against NPY and the vesicular NE and ACh transporters VMAT2 and vesicular acetylcholine transporter (VAChT), we investigated the effect of ciliary neurotrophic factor (CNTF) or long (three weeks) culture periods on the segregation of VMAT2, VAChT, and NPY to separate varicosities. We found that although ganglionic neurons showed cell body coexpression of all the markers examined after three days, VMAT2 was segregated from VAChT in 43% of the VAChT‐positive varicosities. In contrast, VMAT2 was only segregated from NPY in 16.3% of the NPY‐positive varicosities. Cotransmitter segregation and VAChT expression was potentiated by both CNTF and longer times in culture. We also found two types of varicosities: one was smaller and located further from neuronal somata, and the other was larger, proximal to neuronal somata and had a higher level of segregation. These data demonstrate segregation of classical transmitters in sympathetic neurons and plasticity of neurotransmitter segregation. Finally, we discuss a possible functional correlate of segregation in sympathetic neurons. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 70: 913–928, 2010
The Postsecondary Achievement of Participants in Dual Enrollment: "An Analysis of Student Outcomes in Two States".

Discrimination: Title VI of the Civil Rights Act of 1964 states: " No person in the United States shall, on the ground of race, color, or national origin, be excluded from participation in, be denied the benefits of, or be subjected to discrimination under any program or activity receiving federal financial assistance. " Title IX of the Education Amendment of 1972 states: " No person in the United States shall, on the basis of sex, be excluded from participation in, be denied the benefits of, or be subjected to discrimination under any education program or activity receiving federal financial assistance. " Therefore , the National Dissemination Center for Career and Technical Education and the National Research Center for Career and Technical Education, like every program or activity receiving financial assistance from the U.S. Department of Education, must be operated in compliance with these laws. Now office, and the CUNY Office of Institutional Research for providing the data used in this report. Use of these data should not be considered an endorsement of the findings by either Florida or CUNY. The authors are also grateful for the financial support of the National Research Center for Career and Technical Education. Dual enrollment programs enable high school students to enroll in college courses and earn college credit. Once limited to high-achieving students, such programs are increasingly seen as a means to support the postsecondary preparation of average-achieving students. Moreover, though dual enrollment programs typically have been reserved for academically-focused students , increasing numbers of career and technical education (CTE) programs are providing such opportunities to their students. Despite the popularity and growth of dual enrollment programs, little is known about their efficacy. This report seeks to answer several questions regarding their effectiveness using rigorous quantitative methods. We examine the impact of dual enrollment participation for students in the State of Florida and in New York City. For both locations, we specifically examine postsecondary outcomes for participating CTE students; in Florida, we also examine the outcomes of dual enrollment participation for all students. We provide evidence that dual enrollment is a useful strategy for encouraging postsecondary success for all students, including those in CTE programs. Dual enrollment programs are collaborative efforts between high schools and colleges in which high school students (usually juniors and seniors) are permitted to enroll in college courses. These programs provide students with a challenging academic experience and the opportunity …
Experimental validation of the SVD based approach to compensate the positioning errors in a nonredundant spherical NF-FF transformation

An efficient near-field - far-field (NF-FF) transformation with spherical scanning for long antennas, requiring a minimum number of irregularly spaced data, is experimentally validated in this work. It is based on a nonredundant sampling representation of the voltage measured by the probe, obtained by assuming the antenna under test as enclosed in a prolate ellipsoid, a modelling particularly suitable for electrically long antennas. The singular value decomposition method is employed to recover the NF data at the points fixed by the sampling representation from the acquired nonuniformly distributed ones, which are assumed to lie on irregularly spaced parallels. Once the uniform NF data have been reconstructed, those needed by the classical spherical NF-FF transformation are efficiently evaluated by an optimal sampling interpolation formula. Some experimental results, performed at the Antenna Characterization Lab of the University of Salerno and assessing the effectiveness of such a technique for compensating known positioning errors, are shown.
Paramagnetic viral nanoparticles as potential high‐relaxivity magnetic resonance contrast agents

In order to compensate for the inherent high threshold of detectability of MR contrast agents, there has been an active interest in the development of paramagnetic nanoparticles incorporating high payloads of Gd3+ with high molecular relaxivities. Toward this end, the protein cage of Cowpea chlorotic mottle virus (CCMV), having 180 metal binding sites, is being explored. In vivo CCMV binds Ca2+ at specific metal binding sites; however, Gd3+ can also bind at these sites. Using fluorescence resonance energy transfer we have characterized the binding affinity of Gd3+ to the metal binding sites by competition experiments with Tb3+. The measured dissociation constant (Kd) for Gd3+ bound to the virus is 31 μM. The T1 and T2 relaxivities of solvent water protons in the presence of Gd3+‐bound CCMV were 202 and 376 mM−1 s−1, respectively, at 61 MHz Larmor frequency. The unusually high relaxivity values of the Gd3+–CCMV are largely a result of the nanoparticle virus size and the large number of Gd3+ ions bound to the virus. These preliminary results should encourage further investigations into the use of viral protein cages as a new platform for MR contrast agents. Magn Reson Med, 2005. Published 2005 Wiley‐Liss, Inc.
Isolation, Characterization, Molecular Gene Cloning, and Sequencing of a Novel Phytase from Bacillus subtilis

ABSTRACT The Bacillus subtilis strain VTT E-68013 was chosen for purification and characterization of its excreted phytase. Purified enzyme had maximal phytase activity at pH 7 and 55°C. Isolated enzyme required calcium for its activity and/or stability and was readily inhibited by EDTA. The enzyme proved to be highly specific since, of the substrates tested, only phytate, ADP, and ATP were hydrolyzed (100, 75, and 50% of the relative activity, respectively). The phytase gene (phyC) was cloned from the B. subtilis VTT E-68013 genomic library. The deduced amino acid sequence (383 residues) showed no homology to the sequences of other phytases nor to those of any known phosphatases. PhyC did not have the conserved RHGXRXP sequence found in the active site of known phytases, and therefore PhyC appears not to be a member of the phytase subfamily of histidine acid phosphatases but a novel enzyme having phytase activity. Due to its pH profile and optimum, it could be an interesting candidate for feed applications.
Staging of colorectal cancer using contrast-enhanced multidetector computed tomographic colonography.

INTRODUCTION Preoperative staging is essential for the optimal treatment and surgical planning of colorectal cancers. This study was aimed to evaluate the accuracy of colorectal cancer staging done using contrast-enhanced multidetector computed tomographic colonography (CEMDCTC). METHODS We recruited 25 patients with 28 proven colorectal cancers. A 16-slice multidetector computed tomography scanner was used to generate two-dimensional multiplanar reformatted sagittal, coronal and oblique coronal images, and three-dimensional virtual colonography (endoluminal) images. Axial and reformatted views were analysed, and TNM staging was done. Patients underwent surgery and conventional colonoscopy, and surgical histopathological correlation was obtained. RESULTS The diagnostic accuracies for TNM colorectal cancer staging were 92.3% for T staging, 42.3% for N staging and 96.1% for M staging using CEMDCTC. There was excellent positive correlation for T staging between CEMDCTC and both surgery (κ-value = 0.686) and histopathology (κ-value = 0.838) (p < 0.0001), and moderate positive correlation for N staging between CEMDCTC and surgery (κ-value = 0.424; p < 0.0001). The correlation between CEMDCTC and histopathology for N staging was poor (κ-value = 0.186; p < 0.05); the negative predictive value was 100% for lymph node detection. Moderate positive correlation was seen for M staging between CEMDCTC and both surgery (κ-value = 0.462) and histopathology (κ-value = 0.649). No false negatives were identified in any of the M0 cases. CONCLUSION CEMDCTC correlated well with pathologic T and M stages, but poorly with pathologic N stage. It is an extremely accurate tool for T staging, but cannot reliably distinguish between malignant lymph nodes and enlarged reactive lymph nodes.
Growth hormone treatment in children with Noonan's syndrome: four year results of a partly controlled trial

The aim of the study was to evaluate the effect of continuous and discontinuous growth hormone treatment in Noonan's syndrome (NS) on linear growth and bone maturation. Thirty‐seven children with NS aged between 5.4 and 17.5 y were treated with growth hormone (GH) in a dose of 0.15 IUkg−1 per day; 23 of these children were randomly assigned to one of 2 groups in a 3 y partly controlled prospective multicentre study. Group A (n= 8) immediately started GH treatment and after 2 y discontinued GH treatment for 1 y; group B (n= 15) served as a control group during the first year and started GH treatment after 1 y. After the 3 y study period, 17 out of the 23 children continued GH treatment. An additional 14 children (group C) were treated according to the same protocol, but without discontinuation of GH treatment. The effect of GH treatment for up to 3 y was evaluated in terms of gain in height standard deviation score H‐SDS) for calendar age and for bone age. Gain in H‐SDS over the first year was significantly higher in the GH treatment group (+0.5) than in the non‐treated group (+0.0); mean bone maturation was significantly faster in the GH treatment group (1.2 vs 0.5 y/y) Discontinuation in group A in the third study year resulted in catch‐down growth (mean Δ H‐SDS ‐0.2). Over 3 y of GH treatment, mean AH‐SDS for calendar age was not significantly different between discontinuous (A: +0.8) and continuous treatment (B: +0.8; C: +1.2). Mean gain in H‐SDS for bone age in the 3 groups (+0.2, 0.0, +0.3) was minimal after 3 y of GH treatment.
Overdetermined blind source separation using approximate joint diagonalization

Blind separation of mixtures has been achieved by approximate joint diagonalization (AJD) approaches. This paper presents an approach for overdetermined blind source separation (BSS) using AJD. The approach is based on an alternative minimization of the indirect and direct least-squares criteria to the diagonal matrices in the first phase and to the mixing matrix in the second phase, respectively. Simulation result demonstrates that the proposed algorithm is capable for achieving better separation performance in overdetermined mixtures than in determined mixtures at reduced complexity.
Pin reduction and fixation of volar fracture fragments of distal radius fractures via the flexor carpi radialis tendon.

BACKGROUND The objective of this study was to evaluate a technique for reduction and stabilization of residually displaced volar fragments in intraarticular distal radius fractures. METHODS A consecutive series of patients with AO type C3 distal radius fractures treated by one surgeon were studied. Percutaneously placed pins were placed through the flexor carpi radialis tendon to reduce and stabilize volar fracture fragments of distal radius fractures when closed reduction was unsuccessful. The goal of treatment was to achieve less than 2 mm of articular congruity. Postoperative physiotherapy was protocol-based. A validated outcome measurement was used to evaluate patients. RESULTS Of 117 patients, 10 met the inclusion criteria. In all patients, a successful reduction of the volar fragment was achieved with less than 2 mm of residual articular step-off. Reduction was maintained in 8 patients. Follow-up averaged 29 months, and there were no complications associated with the technique. All patients were satisfied with the treatment. CONCLUSION Although the final outcome of patients with this type of fracture depends on many factors, in the small series of patients described, a satisfactory reduction was possible using the describe technique. Transtendinous pinning is a new, undescribed technique that is useful in the treatment of such specific injuries.
Membrane Permeabilization by Poliovirus Proteins 2B and 2BC*

Poliovirus infection leads to drastic alterations in membrane permeability late during infection. Transient expression of each nonstructural protein of poliovirus by means of recombinant vaccinia virus encoding the T7 RNA polymerase indicates that proteins 2B and 2BC strongly enhance membrane permeability to hygromycin B in HeLa cells. Almost no effect on expression of proteins 2C, 3A, 3AB, and 3C was found. Deletions and point mutations in 2B and 2BC have identified sequences in 2B involved in membrane permeabilization. Regions located at both ends of 2B are necessary to bring about these permeability alterations. A deletion of 11 amino acids of 2BC at the junction between 2B and 2C, as well as long deletions in 2C encompassing the GTPase motifs of this protein, do not impair the capacity of 2BC to modify the permeability of the membrane. The release of compounds such as choline or uridine from preloaded cells is also augmented by 2B and 2BC expression.
PDK1 Homologs Activate the Pkc1–Mitogen-Activated Protein Kinase Pathway in Yeast

ABSTRACT PDK1 (phosphoinositide-dependent kinase 1) is a mammalian growth factor-regulated serine/threonine kinase. Using a genetic selection based on a mutant form of the yeast MAP kinase kinase Ste7, we isolated a gene, PKH2, encoding a structurally and functionally conserved yeast homolog of PDK1. Yeast cells lacking bothPKH2 and PKH1, encoding another PDK1 homolog, were nonviable, indicating that Pkh1 and Pkh2 share an essential function. A temperature-sensitive mutant, pkh1D398Gpkh2, was phenotypically similar to mutants defective in the Pkc1–mitogen-activated protein kinase (MAPK) pathway. Genetic epistasis analyses, the phosphorylation of Pkc1 by Pkh2 in vitro, and reduced Pkc1 activity in the pkh1D398G pkh2mutant indicate that Pkh functions upstream of Pkc1. The Pkh2 phosphorylation site in Pkc1 (Thr-983) is part of a conserved PDK1 target motif and essential for Pkc1 function. Thus, the yeast PDK1 homologs activate Pkc1 and the Pkc1-effector MAPK pathway.