Miracle-dz/UniFAR-FTUs huggingface dataset:
Role of heat shock protein 60 (HSP60) on paraquat intoxication

The possibility of establishing a new method of treatment against pulmonary fibrosis caused by acute paraquat intoxication, which takes into consideration the role of heat shock protein 60 (HSP60), was investigated in paraquat‐exposed rat lung mitochondria. In polyacrylamide electrophoresis, mitochondrial protein bands appeared, especially in the range of molecular weight 60 kDa and higher, whereas protein bands disappeared in the 20–40 kDa range on the 4th day after paraquat exposure. The protein profile was normalized on the 7th day and no remarkable changes were seen thereafter up to the 56th day. The changes seen during the observation period were thought to reflect the course of paraquat‐induced dysfunction and subsequent repair. The malondialdehyde concentration in mitochondria decreased until the 7th day but subsequently increased and recovered to normal levels by the 56th day. The relative density of HSP60 increased until the 7th day but subsequently decreased and recovered to normal levels by the 56th day. These two parameters therefore showed symmetrical changes. The change in the malondialdehyde concentration was thought to reflect the course of activation of the antioxidation function in mitochondria and the progression of repair. The change in the relative density of HSP60 was thought to have increased to repair the proteins affected by the paraquat radical and to have normalized with the progression of healing. These results suggest that HSP60 may play an important role in preventing the progression of pulmonary fibrosis induced by paraquat. Copyright © 2001 John Wiley & Sons, Ltd.
Development and characterization of microsatellite markers for parentage analyses of the coral reef damselfish ( Pomacentrus amboinensis : Pomacentridae ) Microsatellite makers for coral reef damselfish

Five new polymorphic microsatellite loci were developed for the coral reef damselfish Pomacentrus amboinensis. Twenty-four individuals from two Great Barrier Reef populations were genotyped at the five loci, with numbers of alleles per locus ranging from 6–23 and observed heterozygosity between 0.42–0.92. In addition, the cross-species testing of six primers developed for Stegastes partitus revealed one primer (SpGATA40) that was also polymorphic for P. amboinensis. Due to high levels of polymorphism (‡14 alleles) in at least four of the six loci and a high proportion of tetranucleotide repeats, these microsatellite markers should be useful for parentage assignment as well as other investigations of individual relatedness.
EARLY MALADAPTIVE SCHEMAS , ATTACHMENT , NEGATIVE AFFECT AND RELATIONSHIP SATISFACTION 2007 / 08

Nicole McDermott EARLY MALADAPTIVE SCHEMAS, ATTACHMENT, NEGATIVE AFFECT AND RELATIONSHIP SATISFACTION 2007/08 Jim A. Haugh, Ph.D. Master of Arts in Mental Health Counseling and Applied Psychology This study examines early maladaptive schemas (EMSs), attachment styles, depression and anxiety, and relationship satisfaction. A sample of 47 (61.7% females, 38.3% males) college students who were in a current relationship completed standardized measures for each study variable. As predicted, depression and EMSs were related to decreased relationship satisfaction. Unexpectedly, attachment styles were under-represented and therefore could not be examined in the study. Contrary to hypotheses, anxiety and relationship satisfaction were not significantly related to one another. ACKNOWLEDGEMENTS Thank you to my thesis advisor, Dr. Haugh, and fellow student colleagues for working with me and helping me through this process. Thanks also to my family and friends who provided me with support and encouragement.
Large Amplitude Elastic Motions in Proteins from a Single-Parameter, Atomic Analysis.

Normal mode analysis (NMA) is a leading method for studying long-time dynamics and elasticity of biomolecules. The method proceeds from complex semiempirical potentials characterizing the covalent and noncovalent interactions between atoms. It is widely accepted that such detailed potentials are essential to the success of NMA’s. We show that a single-parameter potential is sufficient to reproduce the slow dynamics in good detail. Costly and inaccurate energy minimizations are eliminated, permitting direct analysis of crystal coordinates. The technique can be used for new applications, such as mapping of one crystal form to another by means of slow modes, and studying anomalous dynamics of large proteins and complexes. [S0031-9007(96)01063-0] PACS numbers: 87.15. By, 87.15. He Thermal equilibrium fluctuations of the x-ray crystal coordinates of proteins provide a basis for understanding the complex dynamics and elasticity of biological macromolecules [1]. Analysis of the normal modes of globular proteins shows an interesting anomaly. The density of the slow vibrational modes is proportional to their frequency, gsv d, v, rather than gsv d, v 2 as predicted by Debye’s theory [2]. Yet, the atoms in globular proteins are packed as tightly as in solids. We show that a single-parameter potential reproduces the slow elastic modes of proteins obtained with vastly more complex empirical potentials. The simplicity of the potential permits greater insight and understanding of the mechanisms that underlie the slow, anomalous motions in biological macromolecules such as proteins. To date, normal modes of globular proteins have been used to reproduce crystallographic temperature factors [3] and diffuse scatter [4]. Normal mode analyses (NMA’s) shed light on shear and hinge motions necessary for catalytic reactions, and have been used with some success to map one crystal form of a protein into another [5]. Finally, NMA’s yield macroscopic elastic moduli of large protein assemblies, based on their microscopic structure [6]. NMA studies of macromolecules are handicapped, however, by the complex phenomenological potentials used to model the covalent and nonbonded interactions between atom pairs. The necessary initial energy minimization requires a great deal of computer time and memory, and is virtually impossible for even moderately large proteins (with typically thousands of degrees of freedom) with a reasonable degree of accuracy. This inevitably leads to unstable modes which must be eliminated through elaborate methods, and which cast doubts on the validity of the analysis. Moreover, partly because the minimization is carried out in vacuo, the final configuration disagrees with the known crystallographic structure, complicating the interpretation of the results of NMA. A typical example of a semiempirical potential used in molecular dynamics studies and NMA’s has the form [7] Ep › 1 X bonds Kbsb 2 b0d 2 1 1 X angles Kus u2u 0 d 2
Small Heat Shock Protein Hsp 17 . 8 Functions as an AKR 2 A Cofactor in the Targeting of Chloroplast Outer Membrane Proteins in Arabidopsis 1 [ W ] [ OA ]

Plastid proteins that are encoded by the nuclear genome and synthesized in the cytosol undergo posttranslational targeting to plastids. Ankyrin repeat protein 2A (AKR2A) and AKR2B were recently shown to be involved in the targeting of proteins to the plastid outer envelope. However, it remains unknown whether other factors are involved in this process. In this study, we investigated a factor involved in AKR2A-mediated protein targeting to chloroplasts in Arabidopsis (Arabidopsis thaliana). Hsp17.8, a member of the class I (CI) cytosolic small heat shock proteins (sHsps), was identified in interactions with AKR2A. The interaction between Hsp17.8 and AKR2A was further confirmed by coimmunoprecipitation experiments. The carboxylterminal ankyrin repeat domain of AKR2A was responsible for AKR2A binding to Hsp17.8. Other CI cytosolic sHsps also interact with AKR2A to varying degrees. Additionally, Hsp17.8 binds to chloroplasts in vitro and enhances AKR2A binding to chloroplasts. HSP17.8 was expressed under normal growth conditions, and its expression increased after heat shock. Hsp17.8 exists as a dimer under normal physiological conditions, and it is converted to high oligomeric complexes, ranging from 240 kD to greater than 480 kD, after heat shock. High levels of Hsp17.8 together with AKR2A resulted in increased plastid targeting of Outer Envelope Protein7 (OEP7), a plastid outer envelope protein expressed as a green fluorescent protein fusion protein. In contrast, artificial microRNA suppression of HSP17.8 and closely related CI cytosolic sHSPs in protoplasts resulted in a reduction of OEP7:green fluorescent protein targeting to plastids. Based on these data, we propose that Hsp17.8 functions as an AKR2A cofactor in targeting membrane proteins to plastid outer membranes under normal physiological conditions.
Continuous hoops for transverse reinforcement of reinforced concrete columns *

A re-bar fabrication method using continuously wound rectangular or polygonal ties (or continuous hoops) was developed to enhance constructability and economy of reinforced concrete columns. In the present study, cyclic tests were performed to investigate the structural performance of the columns with continuous hoops. The test parameters were the shape, spacing, and bar-diameter of the continuous hoops, and the tolerance between the longitudinal bars and the tie hoops. The load-carrying capacity, deformation capacity, and failure mode of the specimens were directly compared with those of the columns with conventional ties. On the basis of the test results, the effect of the continuous ties was evaluated. Further, the spacing requirement of transverse reinforcement was studied to restrain the post-yield buckling of longitudinal bars in the plastic hinge.
Oxytocin differently regulates pressor responses to stress in WKY and SHR rats: the role of central oxytocin and V1a receptors

Abstract The role of central oxytocin in the regulation of cardiovascular parameters under resting conditions and during acute stress was investigated in male normotensive Wistar-Kyoto (WKY; n = 40) and spontaneously hypertensive rats (SHR; n = 28). In Experiment 1, mean arterial blood pressure (MABP) and heart rate (HR) were recorded in WKY and SHR rats at rest and after an air-jet stressor during intracerebroventricular (ICV) infusions of vehicle, oxytocin or oxytocin receptor (OTR) antagonist. In Experiment 2, the effects of vehicle, oxytocin and OTR antagonist were determined in WKY rats after prior administration of a V1a vasopressin receptor (V1aR) antagonist. Resting MABP and HR were not affected by any of the ICV infusions either in WKY or in SHR rats. In control experiments (vehicle), the pressor response to stress was significantly higher in SHR. Oxytocin enhanced the pressor response to stress in the WKY rats but reduced it in SHR. During V1aR blockade, oxytocin infusion entirely abolished the pressor response to stress in WKY rats. Combined blockade of V1aR and OTR elicited a significantly greater MABP response to stress than infusion of V1a antagonist and vehicle. This study reveals significant differences in the regulation of blood pressure in WKY and SHR rats during alarming stress. Specifically, the augmentation of the pressor response to stress by exogenous oxytocin in WKY rats is caused by its interaction with V1aR, and endogenous oxytocin regulates the magnitude of the pressor response to stress in WKY rats by simultaneous interaction with OTR and V1aR.
Comment on : In Sample Inference and Forecasting in Misspeci fi ed Factor Models

Data mining in economics has become an important topic. A key reason for this is that socalled “big data” dominates the data rich environments in which empirical economists operate. This holds true in fields ranging from macroeconomics, where hundreds of time series are often jointly modelled, to finance, where interest lies in analyzing high frequency data. Of course, big data environments have long been available in various other areas in economics. For example, there are many data rich surveys available to practitioners, such as the PSID, which is a large longitudinal panel study begun in 1968. With so much data now available, attention has turned in recent years to the refinement of old (but relevant) empirical tools and the development of new ones. One of the oldest empirical tools is factor analysis. As is widely known, in 1904 Charles Spearman posited that there was a hidden or latent structure underlying human intelligence. In his model, he assumed that there was one latent common factor, called general intelligence (say ). He argued that particular mental abilities (features) for person , say  could be predicted using the model  =  +  Assuming that the common factor is zero mean and that () = 0, for any  implies that the correlation between feature · and  is simply  Thus, features · and · have correlation equal to the product of their factor loadings (say  = ). This in turn implies that for any four features,    and 
Improving dictionary accessibility by maximizing use of available knowledge

La recherche de mots inconnus dans un dictionnaire est particulierement difficile en japonais car elle demande de connaitre la prononciation correcte de ces mots. Nous proposons un systeme qui supplee a une connaissance partielle de la prononciation des mots en permettant a des apprenants du japonais de rechercher des mots selon une estimation peut-etre incorrecte de leur prononciation. Cela constitue un progres notable par rapport aux systemes existants qui ne proposent aucun traitement en cas de prononciation incorrecte. A cette fin nous calculons d'abord les prononciations possibles de chaque kanji (sinogramme), et les differents types d'alternance phonologique et d'erreur de prononciation qui peuvent se produire et nous leur associons une probabilite. Partant de ces probabilites et de frequences calculees en corpus, nous calculons une mesure de plausibilite pour chaque prononciation generee pour une entree de dictionnaire donnee, selon le modele bayesien naif. Une hypothese de prononciation etant entree par l'utilisateur, le systeme propose un choix d'entrees candidates trouvees dans le dictionnaire. Ce systeme est heberge sur un site web et libre d'acces. Dans une evaluation effectuee sur les donnees du Japanese Proficiency Test (test de competence linguistique d'apprenants du japonais) et sur des erreurs relevees en lecture naturelle, le systeme a reduit de facon significative le nombre de recherches infructueuses dans le dictionnaire dues a des prononciations incorrectes.
Case-control study of apoE gene polymorphism in young CHD patients and controls in the Serbian population

Apolipoprotein E displays polymorphism with three common alleles, e2, e3, and e4. The aim of this research was to determine apoE gene polymorphism in a group of healthy patients and a group of patients with CHD, and to reveal the relation between anthropometric and biochemical parameters and the apoE genotype. In CHD group significantly higher values of blood pressure, waist circumference, BMI and fat %, triglycerides, insulin (HOMA IR) and CRP were found. A statistically significant higher presence of the e3e4 genotype and e4 allele was detected in the CHD group. Statistically significant differences between waist circumference, BMI, insulin and HOMA IR were found between subjects with e3e3 and e3e4 genotypes.
Differentiation , Carry-over , and the Distributed Characteristic in Bilinguals : Structural " Mixing " of the Two Languages ? 1

What level of interaction is there between a bilingual's two linguistic systems? Previous research is mixed on this question. Some researchers have posited an influence of one language on the other (Swain & Wesche 1975); some have suggested that there is influence in some areas, but not in others (Meisel 1986, Paradis 2000); and some have posited that bilinguals' grammars lie "in between" monolinguals' of either language (Hernandez, Bates, & Avila 1994, Kilborn & Ito 1989, McDonald 1987). Perhaps the mixed results on this question can be attributed in part to differing definitions of a bilingual. There are considerable differences across studies in the age at which the participants began acquiring the two languages in question. Many studies could probably more properly be seen as addressing issues of second-language acquisition instead of bilingualism per se. Another difference across studies in the literature concerns the type of exposure the participants have had to their two languages. Has there been equal (frequency of) exposure to the two languages? At all ages? Has one language been learned in an educational setting only? In addition, there is variation in the ages of subjects examined across studies. The present study aims to examine the question of interaction between the two syntactic systems in bilinguals. The data come from two populations. The two populations share the following characteristics: 1. Both populations exist in community contexts in which both languages are alive and spoken extensively on a daily basis in the community. The first bilingual group live in Miami-Dade, in South Florida, where Spanish and English are spoken by a large proportion of the population. The second group live in the Gwynedd/Anglesey counties in North Wales, where Welsh and English are spoken by a substantial proportion of the population (see, e.g., Deuchar this volume). 2. The second characteristic of the groups to be studied here is that a large proportion of the bilingual population have lived in the area since birth. The studies reported here on bilingual children's use of their languages have been restricted to children born in the given communities. Thus, for example, although there is a constant influx of new immigrants into Miami-Dade, the study to be reported excluded children born outside the community. The North Wales community is a fairly stable bilingual community, and its bilingualism is not due to recent immigration into the community. 3. Finally, both studies to be reported here controlled for exposure to the languages in question. In each case, children were assigned to distinct groups on the basis of the language(s) spoken in the home and on the basis of the language(s) used as a medium of education in the school. The data from Miami-Dade come from a larger project (Oller & Eilers 2002b) on the acquisition of language and literacy in the Miami-Dade
Genetic influences on osteoarthritis in women: a twin study

Abstract Objectives: To assess the relative contribution of genetic and environmental factors to common forms of osteoarthritis of the hands and knees. Design: Classic twin study with unselected twins who were screened radiologically for osteoarthritis. Subjects: 130 identical and 120 non-identical female twins aged 48-70 recruited from a London based twin register and through a national media campaign. Main outcome measures: Similarity in identical compared with non-identical twin pairs for radiographic changes at the interphalangeal and first carpometacarpal joints of the hands and the tibiofemoral joint and patellofemoral joint of the knee expressed as intraclass correlations. Results: The intraclass correlations of radiographic osteophytes and narrowing at most sites and the presence of Heberden's nodes and knee pain were higher in the identical pairs. The intraclass correlation of the total radiographic osteoarthritis score in identical pairs (rMZ) was 0.64 (SE 0.05) compared with 0.38 (0.08) in non-identical pairs. The proportion of genetic variance of total osteoarthritis score (osteophytes and narrowing) with modelling techniques was estimated at 0.54 (95% confidence interval 0.43 to 0.65) and ranged from 0.39 to 0.65 for different sites and features (P<0.001) after adjustment for age and weight. Conclusions: These results demonstrate for the first time a clear genetic effect for radiographic osteoarthritis of the hand and knee in women, with a genetic influence ranging from 39-65%, independent of known environmental or demographic confounders. The results of this study should lead to further work on isolating the gene or genes involved in the pathogenesis of this common disabling disease. Key messages Key messages Although environmental factors have traditionally been thought to be the main influences, there are few data to support this Between 39% and 65% of osteoarthritis in the general population can be attributed to genetic factors Identification of the genes concerned could have a large impact on the disease in terms of prevention and new therapeutic approaches
Sonodynamic and Photodynamic Therapy in Advanced Breast Carcinoma: A Report of 3 Cases

Photodynamic therapy (PDT) is an established therapeutic method, first approved by the FDA for certain kinds of cancer in 1998. There are also increasing data to show that a related procedure, sonodynamic therapy (SDT), is a promising new modality for cancer treatment. Here, the authors report clinical results in 3 advanced refractory breast cancer patients who were treated using a combination of sonodynamic and photodynamic therapy (SPDT), along with conventional therapies. All 3 patients had pathologically proven metastatic breast carcinoma. These widely disseminated carcinomas had ultimately failed to respond to conventional therapy. A new sensitizing agent, Sonoflora 1™ (SF1) was administered sublingually; then, after a 24-hour delay, patients were treated with a combination of light and ultrasound. All patients had significant partial or complete responses. SPDT is a promising new therapeutic combination for the treatment of breast cancer.
Use of antidepressant medication dUring cognitive and depressive treatment in Brazilian elderly patients : a 180 day program

Objective: there is an association between the severity of depression and functional impairment in the elderly, such that depression may deteriorate individuals’ ability to perform daily activities, such as eating, dressing and personal care. the use of antidepressants may indirectly improve cognition, whereas some suggested that cognitive symptoms did not benefit from antidepressant treatment. Investigate the influence of the long-term use of antidepressants for an average period of 10 months on the cognitive and functional performances and psychiatric symptoms of a determined sample of elderly patients with depressive symptoms. Method: twenty non-dementia patients with complaints of depressive symptoms, aged between 60 and 90 years, were selected. Half of the sample received antidepressant treatment for 180 days or more. clinical evaluations were performed using a cognitive scale, two functional scales and two psychiatric scales. a longitudinal analysis was performed comparing the results of the first and second evaluations (after prolonged use of an antidepressant for 180 days or more). these two were used to qualify and quantify the presence of depressive indicators and neuropsychiatric
The Railway Station as Shopping Destination

Well-exploited business potential at a station can add considerable financial support to the capital or running expenses of a railway system. Furthermore, stations offering multiple functions can improve convenience for railway network users and enhance the overall impression of station use, thereby attracting more users. In this study, the current state of retail premises operated as adjuncts to the Japanese railway network is investigated as a mature model. Our research estimates the scale of the potential business opportunity, quantifies the effect of retail facilities on users’ impressions of stations, clarifies the shopping behavior of railway users, and offers some fundamental insight into the management and operation of these adjunct functions.