Miracle-dz/UniFAR-FTUs huggingface dataset:
Innovative multilayered millimetre-wave antennas for multi-dimensional scanning and very small footprint applications

This paper presents and overviews the state-of-the-art of substrate integrated waveguide (SIW) techniques in the design and realization of innovative, low-cost, low-profile and low-loss (L3) millimeter-wave antenna elements and arrays for various wireless applications. New classes of multilayered antenna systems are proposed to exploit the vertical dimension of planar structures to overcome certain limitations in standard two-dimensional (2-D) topologies. These structures meet the requirement for developing inexpensive, compact millimeterwave antennas antenna systems. Different structures and architectures are theoretically and experimentally studied and discussed for specific space- and ground-based applications. Practical issues such as high-density integration and high-volume manufacturability are also addressed.
Monomerization and ER Relocalization of GRASP Is a Requisite for Unconventional Secretion of CFTR

Induction of endoplasmic reticulum (ER)‐to‐Golgi blockade or ER stress induces Golgi reassembly stacking protein (GRASP)‐mediated, Golgi‐independent unconventional cell‐surface trafficking of the folding‐deficient ΔF508‐cystic fibrosis transmembrane conductance regulator (CFTR). However, molecular mechanisms underlying this process remain elusive. Here, we show that phosphorylation‐dependent dissociation of GRASP homotypic complexes and subsequent relocalization of GRASP to the ER play a critical role in the unconventional secretion of CFTR. Immunolocalization analyses of mammalian cells revealed that the Golgi protein GRASP55 was redistributed to the ER by stimuli that induce unconventional secretion of ΔF508‐CFTR, such as induction of ER‐to‐Golgi blockade by the Arf1 mutant. Notably, the same stimuli also induced phosphorylation of regions near the C‐terminus of GRASP55 and dissociation of GRASP homomultimer complexes. Furthermore, phosphorylation‐mimicking mutations of GRASP55 induced the monomerization and ER relocalization of GRASP55, and these changes were nullified by phosphorylation‐inhibiting mutations. These results provide mechanistic insights into how GRASP accesses the ER‐retained ΔF508‐CFTR and mediates the ER stress‐induced unconventional secretion pathway.
Ehrlichia canis phylogenetic analysis of the Borgo (Corsica) strain.

Direction Re´gionale du Service de Sante´ des Arme´esde Toulon, Toulon Arme´es, FranceEhrlichia canis (E. canis), a Gram-negative obliga-tory intracellular bacterium with a tropism formonocytes and macrophages, is acknowledged asthe primary cause of canine monocytic ehrlichio-sis (CME). First described in 1935 in Algeria byDonatien and Lestoquard, the worldwide diffu-sion of this agent is related to the distribution ofthe brown dog tick, Rhipicephalus sanguineus.Particularly frequent in tropical and subtropicalregions, this tick is also widely present in theMediterranean Basin. Although CME was ﬁrstreported in France in the late 1970s, no molecularcharacterisation of E. canis strains originatingfrom a dog has ever been attempted. The purposeof the present study was to characterise the 16SrRNA genotype of E. canis strains naturallyinfecting French dogs and to compare this strainwith those from other regions. The isolation of thestrain E. canis Borgo from a dog with a history ofchronic CME from Borgo (Corsica) has allowedthe study of his pathogenesis and molecularcharacterisation.Blood samples were drawn from a dog withclinical and haematological signs of ehrlichiosisand inoculated into healthy dogs. Blood asepti-cally collected from the infected dogs was inoc-ulated into MDHSP cells for culture isolation.Genomic DNA was extracted from this cellculture and the 16S rRNA gene of E. canis wasampliﬁed by reverse transcription PCR as previ-ously described and sequenced with an automaticDNA sequencer.Multiple sequence alignments with the refer-ence sequences downloaded from GenBank wereperformed with the
N-{3-[Bis(2-hydroxyethyl)aminomethyl]-5-nitrophenyl}benzamide

The title compound, C18H21N3O5, was prepared by the reaction of 3-benzamido-5-nitrobenzyl methanesulfonate with diethanolamine and is an intermediate in the synthesis of DNA minor-groove-binding polybenzamide agents capable of being conjugated to additional biologically active species. The asymmetric unit contains two independent molecules, which differ only in the orientations of the hydroxyethyl groups. In the crystal structure, intermolecular N—H⋯O and O—H⋯O hydrogen bonds link molecules into one-dimensional chains.
p53 Regulates Toll-Like Receptor 3 Expression and Function in Human Epithelial Cell Lines

ABSTRACT Toll-like receptors (TLRs) are important sensors of microbial pathogens and mediators of innate immune responses. Although the signal transduction of TLRs is well elucidated, their basal regulation is largely unexplored. Here we show that the tumor suppressor p53 positively regulates the transcription of TLR3, a receptor for viral double-stranded RNA and poly(I-C), by binding to the p53 site in the TLR3 promoter. TLR3 expression was lower in HCT116 p53−/− cells than in HCT116 p53+/+ cells. Activation of p53 by 5-fluorouracil increased the TLR3 mRNA in epithelial cell lines with wild-type p53 but not in cell lines harboring mutant p53. Knockdown of p53 by small interfering RNA decreased the TLR3 expression. TLR3 mRNA was also lower in liver and intestine of p53−/− mice than in p53+/+ mice. Furthermore, the poly(I-C)-induced phosphorylation of IκB-α, nuclear translocation of NF-κB, and phosphorylation of interferon regulatory transcription factor 3, were drastically reduced in HCT116 p53−/− cells, indicating a dysregulation of the two signaling pathways governed by TLR3. Consequently, induction of interleukin-8 and beta interferon after poly(I-C) stimulation was impaired in HCT116 p53−/− cells. These results suggest that p53 influences TLR3 expression and function and highlight a role of p53 in innate immune response in epithelial cells.
Concordance in the Interpretation of Cervical Cytology for the Early Diagnosis of Cervical Cancer

Objective: To identify the diagnostic concordance in the interpretation of cervical smears. Material and Methods: Cross-sectional study from October 2011 to January 2013. 50 samples were read by every 4 cyto-technologists who are the total staffs that read and interpret cytological specimens from a public health institution of the city of San Luis Potosi, Mexico. The cytological diagnosis was confirmed by a certified pathologist. Checklist was developed to determine the quality of the interpretation composed of three sections: adequate sample, inadequate sample and cytological diagnosis. The diagnostic report was made by Bethesda System 2001 classification. Diagnostic concordance was measured through the Kappa coefficient. To establish the differences in cytological diagnosis between each cyto-technologist, chi square test was applied. Results: The concordance is acceptable in the classification of samples as inadequate (k = 0.66). The negative diagnosis was a significant concordance between each cytotechnologist and pathologist; however, no case had very good concordance. In the epithelial abnormalities, only the cytotechnologist 2 had significant concordance with the pathologist, however, its concordance is low. In the case of glandular abnormalities, only cytotechnologist 1 had no significant concordance with the pathologist. The percentage of true negatives was 30%, true positives 20%, false negatives 50% and false positives 0 %. There are significant differences between the readings of cyto-technologist 1 and 2 (p < 0.008), between 1 to 4 (p < 0.001), between 2 and 3 (p < 0.05), and finally between 3 and 4 (p < 0.003). Conclusions: The concordance between cyto-technologists and pathologists is below the required minimum level set by national and international standards.
The American Thyroid Association/American Association of Clinical Endocrinologists guidelines for hyperthyroidism and other causes of thyrotoxicosis: a European perspective.

The new management guidelines of the American Thyroid Association (ATA) and American Association of Clinical Endocrinologists (AACE) for the management of hyperthyroidism and other causes of thyrotoxicosis are an important and informative accomplishment (1). The authors of this precisely written, evidence-based document provide 100 clearly written recommendations, summarizing the rationale for each with pertinent references. The following are among the many recent and relevant clinical issues that are extensively discussed.
An Analysis of Trafficking Receptors Shows that CD44 and P-Selectin Glycoprotein Ligand-1 Collectively Control the Migration of Activated Human T-Cells

Selectins guide the traffic of activated T-cells through the blood stream by mediating their tethering and rolling onto inflamed endothelium, in this way acting as beacons to help navigate them to sites of inflammation. Here, we present a comprehensive analysis of E-selectin ligands expressed on activated human T-cells. We identified several novel glycoproteins that function as E-selectin ligands. Specifically, we compared the role of P-selectin glycoprotein ligand-1 (PSGL-1) and CD43, known E-selectin ligands, to CD44, a ligand that has not previously been characterized as an E-selectin ligand on activated human T-cells. We showed that CD44 acts as a functional E-selectin ligand when expressed on both CD4+ and CD8+ T-cells. Moreover, the CD44 protein carries a binding epitope identifying it as hematopoietic cell E- and/or L-selectin ligand (HCELL). Furthermore, by knocking down these ligands individually or together in primary activated human T-cells, we demonstrated that CD44/HCELL, and not CD43, cooperates with PSGL-1 as a major E-selectin ligand. Additionally, we demonstrated the relevance of our findings to chronic autoimmune disease, by showing that CD44/HCELL and PSGL-1, but not CD43, from T-cells isolated from psoriasis patients, bind E-selectin.
Ouabain inhibits placental sFlt1 production by repressing HSP27‐dependent HIF‐1α pathway

Up‐regulation of placental soluble fms‐like tyrosine kinase 1 (sFlt1) contributes to the pathogenesis of preeclampsia. To evaluate novel upstream pathways that regulate placental sFlt1 production, we screened a library of natural compounds (n=502) in human placental cell lines. Here, we report 3 compounds in the cardiac glycoside family, ouabain, gitoxigenin, and digitoxin, that inhibit placental sFlt1 production at nanomolar concentrations in vitro. We further characterized ouabain and demonstrated that it inhibits sFlt1 mRNA and protein expression in human placental cytotrophoblasts and explant cultures in a dose‐ and time‐dependent manner. Ouabain down‐regulated sFlt1 production by inhibiting hypoxia‐inducible factor 1 (HIF‐1α) protein expression in the placenta. Furthermore, we found that phosphorylation of heat‐shock protein 27 (HSP27) was necessary for ouabain to inhibit HIF‐1α translation. In a rat model of pregnancy‐induced hypertension, ouabain reduced mean arterial pressure and enhanced placental HSP27 phosphorylation without any adverse effects on pups. Further studies are needed to explore the usefulness of targeting HIF‐1α/HSP27 pathway in preeclampsia. —Rana, S. , Rajakumar, A. , Geahchan, C. , Salahuddin, S. , Cerdeira, A. S. , Burke, S. D. , George, E. M. , Granger, J. P., Karumanchi, S. , A. Ouabain inhibits placental sFlt1 production by repressing HSP27‐dependent HIF‐1α pathway. FASEB J. 28, 4324–4334 (2014). www.fasebj. org
MemGator — A portable concurrent memento aggregator: Cross-platform CLI and server binaries in Go

The Memento protocol makes it easy to build a uniform lookup service to aggregate the holdings of web archives. However, there is a lack of tools to utilize this capability in archiving applications and research projects. We created MemGator, an open source, easy to use, portable, concurrent, cross-platform, and self-documented Memento aggregator CLI and server tool written in Go. MemGator implements all the basic features of a Memento aggregator (e.g., TimeMap and TimeGate) and gives the ability to customize various options including which archives are aggregated. It is being used heavily by tools and services such as Mink, WAIL, OldWeb. today, and archiving research projects and has proved to be reliable even in conditions of extreme load.
Effect of integrated Yoga module on positive and negative emotions in Home Guards in Bengaluru: A wait list randomized control trial

Background: The beneficial aspect of positive emotions on the process of learning and the harmful affect of negative emotions on coping with stress and health are well-documented through studies. The Home Guards (HGs) are working in a very stressful situation during election, managing traffic and other crowded places. It is quite essential in present day circumstances that they have to manage their emotions and cope up with different stressful situations. Objective: To study the efficacy of integrated Yoga module (IYM) on emotions (positive and negative affect [PA and NA]) of HGs. Methods: A total of 148 HGs both males and females who qualified the inclusion and exclusion criteria were randomly divided into Yoga group (YG) and control groups (CG). The YG had supervised practice sessions (by trained experts) for 1 h daily, 6 days a week for 8 weeks along with their regular routine work whereas CG performing their routine work. Positive affect negative affect scale (PANAS) was assessed before and after 8 weeks using a modified version of PANAS. Results: PA in YG had significantly increased (P < 0.05) whereas it had decreased significantly (P < 0.05) in CG. Other positive effect in YG had significantly increased (P < 0.001), whereas it had decreased significantly (P < 0.001) in CG. NA in YG had significantly decreased (P < 0.001), whereas it had significantly increased (P < 0.001) in CG. Other NA in YG had significantly decreased (P < 0.001), whereas it had significantly increased (P < 0.01) in CG. Conclusions: The results suggested that IYM can be useful for HGs to improve the PA and to decrease NA score. Moreover, IYM is cost-effective and helps HGs for coping up with emotions in stressful situations.
The next revolution in stroke care

Stroke is the second leading cause of death and disability worldwide. Initiatives to decrease the burden of stroke have largely focused on prevention and acute care strategies. Despite considerable resources and attention, the focus on prevention and acute care has not been successful in changing the clinical trajectory for the majority of stroke patients. While efforts to prevent strokes will continue to have an impact, the total burden of stroke will increase due to the aging population and decreased mortality rates. There is strong evidence for the effectiveness of rehabilitation in better managing stroke and its related disabilities. The time has come to shift the attention in stroke care and research from prevention and cure to a greater focus and investment in the rehabilitation and quality of life of stroke survivors. The rebalancing of stroke care and research initiatives requires a reinvestment in rehabilitation and community reintegration of stroke survivors.
Ensembl 2004

The Ensembl (http://www.ensembl.org/) database project provides a bioinformatics framework to organize biology around the sequences of large genomes. It is a comprehensive and integrated source of annotation of large genome sequences, available via interactive website, web services or flat files. As well as being one of the leading sources of genome annotation, Ensembl is an open source software engineering project to develop a portable system able to handle very large genomes and associated requirements. The facilities of the system range from sequence analysis to data storage and visualization and installations exist around the world both in companies and at academic sites. With a total of nine genome sequences available from Ensembl and more genomes to follow, recent developments have focused mainly on closer integration between genomes and external data.
Improving performance of intrusion detection system by applying a new machine learning strategy

The most acute problem for misuse detection method is its inability to detect new kinds of attacks. A better detection method, which uses a new learning strategy, is proposed to solve this problem. A Concept Hierarchy Generation for attack Labels (CHGL) applying relevant feature subset codes clustering, makes common machine learning algorithms learn attack profiles on high concept levels. And that will enable the system detect more attack instances. Experimental results show the advantage of this new method.
Incremental Adoption of Scrum for Successful Delivery of an IT Project in a Remote Setup

Code71 has been developing, maintaining, and supporting a complex Web-based lead management solution for its US-based small-business client- Method Marketing, L.L. C. using a remote Scrum [1] team. In this report, we describe the process that we used internally to implement Scrum practices in a remote delivery environment and share our lessons learned. In order to put our experiences into perspective, we described our incremental adoption of Scrum with the help of maturity stages. These stages are a result of after-thought, rather than a fore-thought or any existing industry standard model.
Revisiting the structural flexibility of the complex p21ras‐GTP: The catalytic conformation of the molecular switch II

The hydrolysis of GTP in p21ras triggers conformational changes that regulate the ras/ERK signaling pathway. An important active site residue is Gln61, which has been found to be mutated in 30% of human tumors. The dynamics of the active site conformation is studied by using molecular dynamics simulation of two independent structures of the GTP‐bound uncomplexed enzyme. Two distinct conformations of the enzyme are observed, in which the side‐chain residue Gln61 is in different orientations. Essential dynamics analysis is used to describe the essential motions in the transition between the two conformations. Results are compared with earlier simulations of p21ras and its complex with GTPase activating protein p21‐GAP. Proteins 2001;45:297–312. © 2001 Wiley‐Liss, Inc.
Analysis of a perforated SIW structure with a rectangular air box and its application to the design of a step-impedance microwave filter

A new technique for reducing the substrate permittivity in a Substrate Integrated Waveguide (SIW) structure is presented, consisting of removing a centered rectangular box of substrate dielectric material, which increases the equivalent waveguide impedance. This technique can be applied to the implementation of high-impedance SIW sections (perforated regions) sandwitched between low-impedance sections (non perforated sections), the first ones acting as impedance inverters in a filter design. The dispersion properties of this perforated SIW structure have been analyzed and compared to that of a homogeneous equivalent waveguide with a defined effective permittivity. Finally, following the described technique for reducing the substrate permitivitty, a filter prototype has been designed and fabricated to demonstrate the feasibility of the proposed method.
The science of climate change

P i p 2 Variations in local Antarctic atmospheric temperature, as derived from oxygen isotopc data, as weU as concentrations of atmospheric carbon dioxidc and methane From Vostok. Antarctica ice core records. The fan that cold dimates aren't mainrainedwithout a depletion of greenhouse gases, and that warm dimates aren't maintained without an excess of these greenhouse gases is evident. Notice aLo that the current level of atmospheric CO2 (370 ppm) is ~ 2 0 % larger than at anyrime during the last 400,000 yun. Similarly, current levels of aunosphcric mcthane CH4 (1750 ppb) are morc dw double the maximum value found in thc 400,000 yea^ m r d . Notice also thar the increase in CO2 from 280 ppm to 370 ppm over the last 150 years, primarily due to fossil fuel burning, is about thc same as the incmase from the depths of the last ice age (21,000 years ago) to 1750 (190 ppm to 280 ppm) (from Petit et al., 1999).
Scanning electrochemical microscopy - a new technique for the characterization and modification of surfaces

Introduction and Basic Concepts Microscopes are devices that allow one to image structures that are too small to see with the naked eye. Conventional microscopes are based upon the interaction of electromagnetic radiation, in the form of light or electron beams, with the sample. In these, resolution is determined by the wavelength of the radiation used. More recently other kinds of microscopes have been devised that form images by moving a small tip on or near a surface and sensing the changes in tip position or some other tip variable. Here the size of the tip and its distance from the substrate limit the available resolution. For example, in scanning tunneling microscopy (STM)’ the tunneling current that flows between an atomically sharp metal tip and a conductive or semiconductive substrate as the tip is scanned within about 1 nm of the surface can show the surface topography and sometimes provide data about surface electronic energy levels. While STM can image with astounding resolution, and in favorable cases atoms can be “seen”, it cannot be used with insulating substrates, and it does not yield information about the chemical nature of a surface. While other scanning microscopes, such as the atomic force and the ion-conductance microscopes,le can image insulators, they are not “chemically sensitive”. Our research group has constructed a new type of scanning-tip-type microscope in which the imaging process depends on the interaction of the substrate with a species electrogenerated at the tip. As discussed below, the response of such a device is firmly grounded on well-established electrochemical principles and it can be used to probe the conductivity of the sample (even when it is an insulator) and its chemical properties as well. In scanning electrochemical microscopy (SECM)2 (this abbreviation is also used for the device), the substrate and the tip are part of an electrochemical cell that also contains reference and auxiliary electrodes (Figure
Peeling the Lemons Problem with Risk Communication for Mobile Apps

Information asymmetry is a common challenge in information security. This information asymmetry arguably exists in app markets, where people do not understand permissions and have little information on the security of apps. It is not feasible to compare apps based on security and privacy in current app stores. Solving this lemons market problem requires the creation of signals that allow users to differentiate between otherwise indistinguishable goods: more or less secure. In the case of mobile app selection, effective signals should distinguish apps from with lower or higher quality in terms of information security and privacy. To function, such signals should be meaningful, available at or before a decisions is made, and easy to understand. We used the lock icon as a cue, due to its connection with security, and implemented a ratings scale based on We developed an extended Play Store that embedded information security signals. We recruited sixty participants to test the interaction using tablets running Jelly Bean with the cues, as well as the standard user ratings, download count, and permissions interface. The result was that participants chose apps with higher security ratings, and accepted apps with lower ratings or lessor download counts to obtain apps with higher security ratings. We conclude with comparing our results to the users’ behavior in Android Market and show how improving security in the Android ecosystem, can be an economic solution to a lemons market challenge.