Miracle-dz/UniFAR-FTUs huggingface dataset:
Lysosomal and Proteasomal Degradation Play Distinct Roles in the Life Cycle of Cx43 in Gap Junctional Intercellular Communication-deficient and -competent Breast Tumor Cells*

The present study was designed to determine the specific roles played by lysosomes and proteasomes in the degradation of Cx43 in both gap junctional intercellular communication-deficient MDA-MB-231 and -competent BICR-M1Rk cells. In MDA-MB-231 cells, immunolocalization and brefeldin A protein transport blocking studies revealed that there was a propensity for newly synthesized Cx43 to be transported to lysosomes. On the other hand, light and electron microscopic analysis of BICR-M1Rk cells showed that Cx43 gap junctions were prevalent with a subpopulation of intracellular Cx43 localized to lysosomes. In both cell types, Western blots revealed a notable increase in total cellular Cx43 in response to lysosome inhibitors. Interestingly, lactacystin inhibition of proteosomal degradation in MDA-MB-231 cells resulted in a marked increase in phosphorylated Cx43 at the expense of non-phosphorylated Cx43, and this change corresponded with an increase in “oversized” gap junction plaques. In BICR-M1Rk cells, lactacystin treatment partially prevented the BFA-induced loss of gap junctions. Together, our data suggests that lysosomes play a key role in not only degrading internalized gap junction in BICR-M1Rk cells but also in degrading Cx43 delivered from early secretory compartments to lysosomes in MDA-MB-231 cells. Overall proteasomal degradation regulates the stability of phosphorylated Cx43 and appears to promote the internalization of Cx43 from the cell surface.
Simultaneous assessment of flow and BOLD signals in resting‐state functional connectivity maps

We have recently demonstrated using functional magnetic resonance imaging the presence of synchronous low‐frequency fluctuations of signal intensities from the resting human brain that have a high degree of temporal correlation (p<0.0001) both within and across the sensorimotor cortex. A statistically significant overlap between the resting‐state functional connectivity map and the task‐activation map due to bilateral finger tapping was obtained. Similar results have been obtained in the auditory and visual cortex. Because the pulse sequence used for collecting data was sensitive to blood flow and blood oxygenation, these low‐frequency fluctuations of signal intensity may have arisen from variations of both. The objective of this study was simultaneously to determine the contribution of the blood oxygenation level signal and the flow signal to physiological fluctuations in the resting brain using the flow‐sensitive alternating inversion recovery pulse sequence. In all subjects, the functional connectivity maps obtained from BOLD had a greater coincidence with task‐activation maps than the corresponding functional connectivity maps obtained from blood‐flow signals at the same level of statistical significance. Results of this study suggest that while variations in blood flow might contribute to functional connectivity maps, BOLD signals play a dominant role in the mechanism that gives rise to functional connectivity in the resting human brain. © 1997 John Wiley & Sons, Ltd.
Post myocardial infarct detection with support vector machine and ECG intervals ratios JTp/JT, Tpe/JTp and Tpe/JT

Myocardial infarct cause myocardial tissue disease increasing the probability to have arrhythmias such as ventricular fibrillation, these cardiac problems are cause of death. This paper implement a support vector machine classifier trained with the JTp/JT, Tpe/JTp and Tpe/JT intervals ratios extracted from ECG signals of healthy patients and patients with post myocardial infarct diagnosis. The accuracy for the system trained with the time ratios above mentioned is around 90% to 100% for both classes, others classifiers implemented 2 to 5 times the amount of input data with other methods and obtaining 80% of accuracy.
Propagation aware statistical modeling of MIMO terminal antennas

We address the statistical modeling of multiple antennas terminals in realistic use conditions. An analysis is carried out of the generation of MIMO channel matrices in the Kronecker approximation, showing that it is valid for a set of multiple antenna terminals only if the generation is carried out independently per each terminal, rather than by using correlation and power shaping matrices computed over the whole channel and terminal set. This is verified both from full simulations and from semi-experimental simulations using measured multiple antenna terminals mockups under strong influence of head and hand.
A review on implementation of bimodal newborn authentication using raspberry Pi

In developing countries the newborn swapping and abduction in hospitals are challenging issue and occurs all over the world. Traditional methods have their own drawbacks. Hence, a newborn personal authentication system is proposed for this issue based on bimodal biometric system wherein footprint of newborn and finger print of their mother is used for recognition. This concept is further enhanced by developing a prototype to be implemented on a raspberry Pi (a single board computer). In this paper, SIFT feature extraction, RANSAC algorithm for matching of fingerprint and footprint biometrics using OpenCV on Raspberry pi is implemented. The raspberry Pi is ARM1176JZFS application processor, System on chip (SoC) denoted as Broadcom BCM2835. It enhances performance, consumes less power, and reduces overall system cost and size. The raspberry Pi is been controlled by a modified version of Debian Linux optimized for ARM architecture. The image recognition is performed using open source OpenCV-2.4. 9 in Linux platform using CMake, g++, makefile. Thereby the proposed system improves the security system in hospitals / birth centers and provides a low cost solution to the newborn swapping rather than the expensive DNA procedures. In this paper, discusses about the research works carried on hardware as a biometric module to enhance the performance of a standalone device.
Age‐specific influence of wheezing phenotypes on pre‐adolescent and adolescent health‐related quality of life

Asthma is associated with diminished health‐related quality of life (HRQoL). Particularly in adolescence, asthma may be under‐diagnosed and undertreated or poorly managed. Therefore, we aimed to determine the association between childhood wheezing phenotypes rather than asthma and adolescent HRQoL in children aged 10–17 yr.
Role of anemia in survival of patients with elderly aggressive non-Hodgkin's lymphoma after chemotherapy

Baseline anemia is a relevant prognostic factor in the overall population of non-Hodgkin's lymphoma (NHL) patients, and studies focusing on elderly NHL are awaited. We conducted a pooled analysis of a cohort of comparable patients enrolled (1993 – 2001) in three multicenter clinical trials on use of a MACOP-B-like regimen (VNCOP-B) for front-line treatment of elderly aggressive NHL. Models of Cox's proportional hazards regression analysis of prognostic value of pre-/post-treatment hemoglobin values in terms of 5-year overall survival included age, sex, initial tumor staging and response to treatment. Of the 168 patients screened, 16 were excluded due to missing data or lack of 5-year follow-up. In addition to achievement of complete/partial remission (adjusted relative risk [RR], 0.215; p = 0.0001) and advanced stage (II-IV vs. I – II; adjusted RR, 1.55; p = 0.0023), post-treatment hemoglobin values were an independent predictor of survival (adjusted RR per 1-g/dL increment, 0.76; p = 0.0041). In the present analysis, pretreatment hemoglobin values were associated with only marginal risk reduction (adjusted RR per 1-g/dL increment, 0.985; p = 0.049). Post-treatment hemoglobin values appear to provide a strong independent predictor of 5-year survival in elderly aggressive NHL, supporting the potential role of anemia correction in this group of patients.
Cytokines and chemokines in the vitreous fluid of eyes with uveal melanoma.

PURPOSE In uveal melanoma, both tumor size and an inflammatory phenotype have been shown to correlate with a poor clinical prognosis. The purpose of this study was to identify whether inflammatory cytokines were present in the vitreous of eyes with uveal melanoma and to determine whether the vitreal concentration of cytokines correlated with prognostic variables such as tumor dimensions and immune cell infiltrate. METHODS Vitreous was acquired from patients with uveal melanoma (n = 33) and from control eyes with no known ocular conditions (n = 9), and analyzed using a 27-plex cytokine bead array. Immunofluorescence testing was performed to determine the presence of macrophages, CD4(+), CD8(+), and Foxp3(+) regulatory T cells (T(regs)). RESULTS Compared with control eyes, eyes with uveal melanoma demonstrated higher vitreal concentrations of many cytokines and chemokines, including IL-6, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, TNF-α, and RANTES. IL-1ra was decreased in the vitreous of tumor-bearing eyes compared with controls. Tumor prominence positively correlated with several cytokines, including IL-6, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, TNF-α, RANTES, GCSF, IFN-γ, and VEGF. IL-6 and IP-10 were found to positively correlate with increasing regulatory T-cell infiltrate and IL-6 alone positively correlated with macrophage infiltration. CONCLUSIONS Eyes with uveal melanoma contain higher vitreal concentrations of several inflammatory cytokines and chemokines that correlate predominantly with increasing tumor size; elevations in certain cytokines and chemokines also correlate with the presence of immune cell infiltrate.
Inhibition of NF-kappaB signaling by fasudil as a potential therapeutic strategy for rheumatoid arthritis.

OBJECTIVE Rheumatoid arthritis (RA) is the most common systemic autoimmune disease and is characterized mainly by symmetric polyarticular joint disorders. The pathologic processes are mediated by a number of cytokines, chemokines, cell adhesion molecules, and matrix metalloproteinases. The expression of most of these molecules is controlled at the transcriptional level. In addition, activation of NF-kappaB is involved in RA pathogenesis. This study was performed to explore the role of a novel serine/threonine kinase inhibitor, fasudil, in the control of the NF-kappaB activation pathway and to investigate the therapeutic effects of fasudil on arthritis development in a rat model of RA. METHODS Fibroblast-like synoviocytes (FLS) from RA patients and human endothelial cells (ECs) were established and maintained. To study the role of fasudil on cytokine expression, various cytokines expressed in the RA FLS and human ECs were measured by enzyme-linked immunosorbent assay following stimulation of the cells with interleukin-1beta (IL-1beta) in the presence of various concentrations of fasudil. The role of fasudil on NF-kappaB activation was studied using a reporter gene assay, Western blotting of IkappaBalpha, immunofluorescence analysis of the p65 subunit of NF-kappaB, and electrophoretic mobility shift assay. The in vivo effects of fasudil on arthritis were studied in a rat adjuvant-induced arthritis (AIA) model. RESULTS Fasudil inhibited cytokine expression in RA FLS and human ECs and also inhibited the activation of ECs, in a dose-dependent manner. Fasudil inhibited IL-1beta-induced activation of NF-kappaB independent of the inhibition of IkappaBalpha degradation and nuclear translocation of NF-kappaB, and inhibited IL-1beta-induced DNA binding of NF-kappaB. Finally, in vivo, fasudil ameliorated arthritis in rats with AIA, without any adverse effects. CONCLUSION Serine/threonine kinase inhibitor fasudil inhibits the development of arthritis in a rat model of RA, and also inhibits the NF-kappaB signaling required for binding of NF-kappaB to specific DNA sequences through, for example, the phosphorylation of p65, suggesting that a specific target of fasudil might be a novel NF-kappaB kinase. Thus, fasudil serves as a novel strategy for the treatment of RA.
Gear Tooth Surface Damage Fault Detection Using Induction Machine Stator Current Space Vector Analysis

A noninvasive technique for the diagnosis of gear tooth surface damage faults based upon the stator current space vector analysis is presented. The torque oscillation profile produced by the gear tooth surface damage fault in the mechanical torque experimented by the driven electrical machine is primarily investigated. This profile consists of a mechanical impact generated by the fault followed by a damped oscillation that can be identified through the mechanical system torsional natural frequency and damping factor. Through theoretical developments, it is shown that the periodic behavior of this particular shape produces fault-related frequencies in the stator current and harmonics integer multiple of the rotation frequency in the stator current space vector instantaneous frequency. The fault signature related to the gear tooth surface damage fault is predicted through the numerical simulation. The simulation results are validated through experimental tests, illustrating a possible noninvasive gear tooth surface damage fault detection with a fault sensitivity comparable to invasive methods. A dedicated experimental setup, which is based on a 250-W squirrel-cage three-phase induction machine that is shaft connected to a single-stage gear, has been used for this purpose.
Exploring the therapeutic value of hope in palliative nursing

Abstract Hope is a multi-dimensional concept that is integral to a dying person's needs. It is an essential resource that assists individuals with a life-threatening illness to cope during times of intense physical and psychological distress. The objective of this article is to explore and analyze the therapeutic value of hope. The phenomenon of hope will be explored through the analysis and application of Dufault and Martocchio's Multidimensional Model of Hope (MMH) to a clinical scenario. Factors determining hope in cancer patients as well as interventions that can foster hope in dying patients will be identified. Discussion includes examination of literature gaps, relevance to nursing practice, and practical strategies to engender hope and thereby enhance quality of life (QOL) in advanced cancer patients.
Daily ambulation activity and task performance in community-dwelling older adults aged 63-71 years with preclinical disability.

BACKGROUND The purpose of this study was to examine differences in daily ambulation activity and task modification between community-dwelling older adults above and below an empirically derived physical threshold that has been linked to independence. METHODS 20 community-dwelling older adults (72.8 +/- 6 years) were categorized into groups based on functional performance using the Continuous scale Physical Functional Performance Test total score (Cs-PFP). Individuals with Cs-PFP > or =57 were assigned to the high functioning group (HIGH; n=10) with all others assigned to the lower functioning group (LOW; Cs-PFP<57; n=10). Dependent variables included steps/day, number of tasks reported with difficulty, and number of tasks reported with modification. RESULTS HIGH took significantly more steps/day (HIGH: 9503 +/- 4623; LOW: 5048 +/- 2917, p=.019) compared to LOW. Groups reported having difficulty with a similar number of tasks (HIGH: 0.4 +/- 1; LOW: 1.0 +/- 1, p=.092) but LOW reported modifying a significantly larger number of tasks (HIGH: 0.3 +/- 1; LOW 1.4 +/- 1, p=.049). CONCLUSIONS Older adults with preclinical disability have reduced daily ambulatory activity compared to older adults with high function despite a similar independent living status. Individuals compensate for reduced physical reserves by modifying the method of performing a task. Identifying early declines in physical ability through task modification and daily ambulation will provide the opportunity for timely intervention to older adults desiring to remain independent within a community-dwelling environment.
Radiology of the retropharyngeal space.

The retropharyngeal space extends from the skull base to the T4 vertebral level and contains the retropharyngeal nodes superiorly and fatty tissue elsewhere. This space is important as it is a potential route for the spread of infection and malignancy. This pictorial essay outlines the anatomy of the retropharyngeal space and illustrates the various disease processes that may be seen in region.Chong, V. F. H. , Fan, Y. F. (2000). Clinical Radiology55, 740-748.
Single-Fiber Recordings of Nociceptive Fibers in Patients With HSAN Type V With Congenital Insensitivity to Pain

Objectives:Nerve growth factor (NGF) is a protein important for growth and survival, but also for modulation of sensitivity of nociceptors and sympathetic neurons. The purpose of the present study was to investigate the effects of reduced NGF signaling in patients with hereditary sensory and autonomic neuropathies type V, congenital insensitivity to pain, caused by a mutation of the NGF&bgr; gene, including a characterization of single nociceptive fibers using microneurography (MNG). Materials and Methods:One homozygote and 2 heterozygote patients with this mutation were examined with electromyography/neurography, thermal testing, quantitative sudomotor axon reflex test, and electrically induced axon reflex erythema in addition to MNG. Results:Low quantitative sudomotor axon reflex test measurements of 0.02 (left foot) and 0.03 (right foot) &mgr;L/cm2 and elevated thermal thresholds for warmth and cold detection testing showed clear impairment of small nerve fibers, both sudomotor efferent and somatic afferent fibers, in the patient homozygote for the mutation. MNG from one of the heterozygote patients revealed changes in the small nociceptive fibers in skin, including abnormally low conduction velocity, spontaneous activity in A-&dgr; fibers and C-nociceptors and abnormal or lacking response to heat. Discussion:The findings of grossly intact pain thresholds compared with anamnestic insensitivity of pain in deep somatic tissue such as bone suggest a gradient of impairment dependent on different NGF availability in various tissues. Even though these patients in some aspects report insensitivity to pain, they also report chronic spontaneous pain as their main symptom, strikingly highlighting differential mechanisms of insensitivity to evoked pain versus spontaneous pain.
Drosophila Pumilio Protein Contains Multiple Autonomous Repression Domains That Regulate mRNAs Independently of Nanos and Brain Tumor

ABSTRACT Drosophila melanogaster Pumilio is an RNA-binding protein that potently represses specific mRNAs. In developing embryos, Pumilio regulates a key morphogen, Hunchback, in collaboration with the cofactor Nanos. To investigate repression by Pumilio and Nanos, we created cell-based assays and found that Pumilio inhibits translation and enhances mRNA decay independent of Nanos. Nanos robustly stimulates repression through interactions with the Pumilio RNA-binding domain. We programmed Pumilio to recognize a new binding site, which garners repression of new target mRNAs. We show that cofactors Brain Tumor and eIF4E Homologous Protein are not obligatory for Pumilio and Nanos activity. The conserved RNA-binding domain of Pumilio was thought to be sufficient for its function. Instead, we demonstrate that three unique domains in the N terminus of Pumilio possess the major repressive activity and can function autonomously. The N termini of insect and vertebrate Pumilio and Fem-3 binding factors (PUFs) are related, and we show that corresponding regions of human PUM1 and PUM2 have repressive activity. Other PUF proteins lack these repression domains. Our findings suggest that PUF proteins have evolved new regulatory functions through protein sequences appended to their conserved PUF repeat RNA-binding domains.
orrelation of Sturtian diamictite successions in southern Australia and orthwestern Tasmania by Re – Os black shale geochronology and the mbiguity of “ Sturtian ”-type diamictite – cap carbonate pairs as hronostratigraphic marker horizons

Recent geochronological studies have raised concerns regarding the accuracy of regional and global correlations of Neoproterozoic sedimentary successions using relative dating methods such as carbon isotope chemostratigraphy and glaciogenic diamictite–cap carbonate lithological and geochemical characteristics. Precise and accurate radio-isotopic age constraints for Neoproterozoic sedimentary rocks thus remain a high priority for establishing a reliable Neoproterozoic chronostratigraphy. Here, we present a new Re–Os age of 640.7 ± 4.7 Ma from black shales of the upper Black River Dolomite (Togari Group, northwestern Tasmania) that represents a minimum age for deposition of the immediately underlying, diamictite-bearing Julius River Member. The upper Black River Dolomite Re–Os age is statistically identi-
Cladispa Baly : revision , biology and reassignment of the genus to the tribe Spilophorini ( Coleoptera : Chrysomelidae : Cassidinae

The genus, Cladispa Baly 1858, is transferred from the tribe Imatidiini (=Cephaloleiini Chapuis, 1875) to Spilophorini Chapuis, 1875 based on the review of type material, newly collected specimens and molecular phylogenetic analysis. The type species, C. quadrimaculata Baly, 1858, is redescribed, and two new species, C. amboroensis sp.n. from Bolivia (Santa Cruz Department) and C. ecuadorica sp.n. from Ecuador (Pastaza Province), are described and figured. The morphology of C. amboroensis sp.n. immature stages is broadly consistent with other Spilophorini. Field observations document that both C. quadrimaculata and C. amboroensis sp.n. are trophic specialists on Orchideaceae. Keys to Cladispa species and Spilophorini genera are provided. Trophic associations of other Cassidinae and Orchideaceae are discussed. This published work has been registered in ZooBank, http://zoobank.org/urn:lsid: zoobank.org:pub:42A1ECF3-2030-4938-8F3D-FE7EC36F303A
Ratings of Perceived Exertion are Not Influenced by Exercise Stage of Change in Physically Active College Students

Comparisons of ratings of perceived exertion based on fitness have been equivocal but have not accounted for recent exercise history. The purpose of this study was to test mean differences in ratings of perceived exertion among individuals of differing exercise histories as measured by exercise stage of change. Participants completed questionnaires to measure exercise stage of change and physical activity [preparation stage (n = 32), action stage (n = 31), and maintenance stage (n = 41)]. Ratings of perceived exertion were measured during a graded maximal exercise test. Individual regression equations were computed to estimate the ratings for 50%, 60%, 70%, and 80% of VO2 max. There were no significant differences in the ratings across the preparation, action, and maintenance stages. Therefore, the hypothesis that recent exercise history would influence ratings of perceived exertion during an aerobic fitness test was rejected.