Miracle-dz/UniFAR-FTUs huggingface dataset:
Channel-forming activity and channel size of the RTX toxins ApxI, ApxII, and ApxIII of Actinobacillus pleuropneumoniae

The determinants of the Actinobacillus pleuropneumoniae RTX toxins ApxI, ApxII, and ApxIII were expressed in an Escherichia coli strain. The toxins were concentrated from the supernatants of cell cultures. The addition of the toxins to the aqueous-phase-bathing lipid bilayer membranes resulted in an increase in the membrane conductance when membranes made of asolectin or phosphatidylethanolamine were used. The toxins were relatively inactive in membranes made of other lipids. The membrane activity (i.e., the number of channels formed at a given Apx concentration) was different for each of the three Apx toxins. That of ApxI, which has the strongest cytotoxic activity, was highest, followed by that of ApxIII and ApxII, which is the least cytotoxic. The conductance increases of ApxIII and ApxII were smaller by factors of 10 and 50, respectively, than that of ApxI under otherwise identical conditions. Single-channel experiments demonstrated that all three Apx toxins formed ion-permeable channels of different conductances. The major open state was approximately the same for the two hemolytic toxins ApxI and ApxII (540 and 620 pS in 0.15 M KCI), whereas the single-channel conductance of the nonhemolytic ApxIII was approximately one-fifth of that of the other two toxins (95 pS). Experiments with different salts suggested that the Apx channels of A. pleuropneumoniae were exclusively cation selective because of negative charges localized at the channel mouth. Analysis of the single-channel data using the Renkin correction factor suggested that the Apx toxins formed aqueous channels with different diameters for the three toxins. Pore-forming properties of the Apx toxins were compared with those of other RTX toxins. All of these toxins have common features and form channels that are transient but have different sizes as judged from the different single-channel conductances.
Reading John Grisham’s Bleachers with Foucault: lessons for sports retirement

ABSTRACT This paper has explored how a sociological reading of John Grisham’s short work of fiction Bleachers can shed light on the complexities of the coaching and sports retirement processes. The aim of this paper was to consider the “sociological insights” that Bleachers has to offer by reading the novel in companionship with the thoughts of French post-structuralist thinker Michel Foucault , specifically his disciplinary analysis from Discipline and Punish. This paper has also observed how the figure of Coach Eddie Rake in Bleachers can be considered as trope for retired athletes’ unresolved feelings towards their former sport. In achieving these aims, this paper has considered how Bleachers, when read through a sociological lens, can act as a meaningful data source for those invested in learning more about the poorly understood phenomena of sports retirement, as well as the dangers of coaching practices that are overly reliant upon the disciplinary logic of sport.
Morphometric analysis of the choroid, Bruch's membrane, and retinal pigment epithelium in eyes with age-related macular degeneration.

PURPOSE To quantify morphologic changes in the choroid, including choriocapillaris and larger choroidal vessels, Bruch's membrane, and retinal pigment epithelium in eyes with age-related macular degeneration (AMD) and late ARM (age-related maculopathy). METHODS The authors analyzed 40 eye bank eyes with late ARM (21 with the neovascular AMD and 19 with nonneovascular AMD) and compared them with 40 age-matched eyes without signs of late ARM (AMD). The eyes were processed for light microscopy, and seven variables were measured in the macular and peripheral regions with a digital filar micrometer. RESULTS There were no statistically significant differences observed between eyes with neovascular versus nonneovalcular AMD. The single most important difference between eyes with and without AMD was the amount of basal laminar deposit (P < 0.001). Eyes with AMD displayed fewer large choroidal vessels in the submacular choroid than eyes without AMD (3.5 +/- 1.5 mm-1 and 5.7 +/- 1.6 mm-1; P < 0.001). The submacular choriocapillaris density was higher in eyes with AMD (0.62 +/- 0.06) than in eyes without AMD (0.51 +/- 0.08; P < 0.001). The diameter of the large choroidal vessels in the peripheral choroid was increased in eyes with AMD (30 +/- 8 microns) compared to eyes without AMD (21.4 +/- 6.2 microns; P < 0.001). The peripheral choriocapillaris density displayed the same pattern as in the macular region in eyes with and without AMD. CONCLUSIONS The amount of basal laminar deposit strongly correlates with the histologic presence of AMD. Eyes with AMD show differences of the density and diameter of choroidal blood vessels compared to eyes without AMD.
Comparison of multiplex reverse transcription polymerase chain reaction and conventional cytogenetics as a diagnostic strategy for acute leukemia

To clarify the usefulness of multiplex reverse transcription polymerase chain reaction (mRT‐PCR) in diagnosing acute leukemia, mRT‐PCR detecting 28 different translocations was performed on bone marrow aspirates of 156 patients with acute leukemia, and the results were compared with conventional chromosomal karyotypes. About 113 of 156 patients had acute myeloid leukemia (AML), and 36 had acute lymphoid leukemia (ALL) with patients’ ages ranging from 1 to 84 (median: 34.5). Concordance rate between karyotyping and mRT‐PCR was 50% (51% in AML and 44% in ALL). Karyotype revealed chromosomal abnormalities in 70 patients (45%) while mRT‐PCR showed some aberrations in 59 patients (38%). mRT‐PCR detected t(1;19), t(4;11), t(9;11), t(10;11), t(11;19), t(12;21), and TAL1d, which were not detected by G‐banding. In addition, 10 patients with t(15;17), one patient with t(8;21), and four patients with t(9;22) detected by mRT‐PCR revealed normal karyotypes. However, mRT‐PCR did not detect numerical abnormalities, deletions, and translocations other than the 28 translocations included in the assay as expected. In conclusion, although it cannot be a substitute of the conventional chromosome analysis, mRT‐PCR could be a complementary diagnostic strategy of acute leukemia.
Minimally invasive neuroradiologic model of preclinical transient middle cerebral artery occlusion in canines

Stroke is currently the third leading cause of death in the United States, with approximately 780,000 Americans affected by a new or recurring stroke each year. Although a variety of therapeutic approaches have shown promise in small-animal models of stroke, the vast majority of clinical trials to test the efficacy of such modalities have failed. To bridge the translational gap between laboratory and clinical research, we developed a preclinical model of acute ischemic stroke in dogs. Using a minimally invasive endovascular approach, a platinum coil was intravascularly guided through the vertebrobasilar system under C-arm fluoroscopy to occlude the M1 segment of the middle cerebral artery (MCA) for 1 h. The approach included femoral artery catheterization to access the MCA and therefore eliminated the occurrence of head trauma associated with other preclinical stroke models relying on transorbital or craniectomy approaches. After 1 h of focal MCA ischemia, the coil was retrieved to cause reperfusion, which was verified by arteriograms. At 24 h, T2-weighted coronal magnetic resonance (MR) images were acquired and processed for three-dimensional reconstruction of the brain and its vasculature. Infarction, limited to the area at risk, was noted. Two independent observers calculated the mean percentage hemispherical lesion volumes as follows: observer 1, 30.9 ± 2.1%; observer 2, 31.2 ± 4.3%. Infarct-affected changes in histology were determined by hematoxylin and eosin as well as by Fluoro-Jade staining. This work reports the successful development of a powerful preclinical model of stroke that lends itself to the study of biologic mechanisms as well as to testing experimental therapeutics.
Anion conductive imidazolium-based Parmax alkaline membrane for fuel cell applications

The anion exchange polyphenylene membrane containing imidazolium hydroxide ionic functional group was synthesized by sequential chloromethylation, substitution with 1-methylimidazole and ion exchange. The imidazolium hydroxide Parmax 1200 membrane has all carbon-carbon bonds without ether linkages, which would be chemically strong. The polyphenylene structure of Parmax provides a stiff and resistant backbone, whereas the pendant benzoyl group provides sites for chemical modifications. The resulting ionomer membrane showed ion exchange capacity (IEC) of 2.14 mmol g-1 with maximum chloromethylation yield. The imidazolium-functionalized copolymer membrane showed lower water affinity and high durability in alkaline condition. It exhibited hydroxide ion conductivity above 10-2 S cm-1 at room temperature and good chemical stability for up to seven days without significant losses of ion conductivity. The structural properties of the synthesized polymer membrane were investigated by 1H NMR spectroscopy and FT-IR. The membranes were studied by IEC, water uptake, and conductivity assessment.
Green Full-Duplex Self-Backhaul and Energy Harvesting Small Cell Networks With Massive MIMO

With the dense deployment of small cell networks, the powering and backhaul problem of small cell base stations (SBSs) has attracted great attention, and energy harvesting technology and self-backhaul technology have been proposed as promising solutions. Although some excellent works have been done on energy harvesting and self-backhaul in small cell networks, most existing works do not consider them jointly. In this paper, we aim at green small cell networks by jointly achieving self-backhaul and energy harvesting. In addition, full-duplex and massive multiple-input and multiple-output technologies are also exploited to enhance the system performance. In order to improve the energy efficiency (EE) further, a novel precoding scheme is designed to eliminate both the inter-tier and multi-user interference. Based on the proposed precoding scheme, we formulate the cell association and power allocation problem as an optimization problem to optimize the system EE performance, with the energy arrival rate and remaining battery energy in SBSs involved. The formulated optimization problem implies a sleep mechanism to control the ON/OFF of SBSs, which will further reduce the energy consumption of small cell networks. In addition, to reduce the computation complexity to solve this non-convex problem, we propose to transform the original problem into a difference of convex program, which can be efficiently solved via a constrained concave convex procedure-based algorithm. Extensive simulation results are presented to justify the effectiveness of the proposed scheme with different system configurations.
Silencing MPK 4 in Nicotiana attenuata Enhances Photosynthesis and Seed Production But Compromises Abscisic Acid-Induced Stomatal Closure and Guard Cell-Mediated Resistance to Pseudomonas syringae pv tomato DC 30001 [ W ]

Mitogen-activated protein kinases (MAPKs) play pivotal roles in development and environmental interactions in eukaryotes. Here, we studied the function of a MAPK, NaMPK4, in the wild tobacco species Nicotiana attenuata. The NaMPK4-silenced N. attenuata (irNaMPK4) attained somewhat smaller stature, delayed senescence, and greatly enhanced stomatal conductance and photosynthetic rate, especially during late developmental stages. All these changes were associated with highly increased seed production. Using leaf epidermal peels, we demonstrate that guard cell closure in irNaMPK4 was strongly impaired in response to abscisic acid and hydrogen peroxide, and consistently, irNaMPK4 plants transpired more water and wilted sooner than did wild-type plants when they were deprived of water. We show that NaMPK4 plays an important role in the guard cellmediated defense against a surface-deposited bacterial pathogen, Pseudomonas syringae pv tomato (Pst) DC3000; in contrast, when bacteria directly entered leaves by pressure infiltration, NaMPK4 was found to be less important in the resistance to apoplast-located Pst DC3000. Moreover, we show that salicylic acid was not involved in the defense against PstDC3000 in wildtype and irNaMPK4 plants once it had entered leaf tissue. Finally, we provide evidence that NaMPK4 functions differently from AtMPK4 and AtMPK11 in Arabidopsis (Arabidopsis thaliana), despite their sequence similarities, suggesting a complex functional divergence of MAPKs in different plant lineages. This work highlights the multifaceted functions of NaMPK4 in guard cells and underscores its role in mediating various ecologically important traits.
Neurobiological Mechanisms of Aggression and Stress Coping: A Comparative Study in Mouse and Rat Selection Lines

Aggression causes major health and social problems and constitutes a central problem in several psychiatric disorders. There is a close relationship between the display of aggression and stress coping strategies. In order to gain more insight into biochemical pathways associated with aggression and stress coping, we assessed behavioral and neurobiological responses in two genetically selected rodent models, namely wild house mice selectively bred for a short (SAL) and long (LAL) attack latency and Wistar rats bred for high (HAB) or low (LAB) anxiety-related behavior. Compared to their line counterparts, the SAL mice and the LAB rats display a high level of intermale aggression associated with a proactive coping style. Both the SAL mice and the LAB rats show a reduced hypothalamic-pituitary-adrenal (HPA) axis response to non-social stressors. However, when exposed to social stressors (resident-intruder, sensory contact), SAL mice show an attenuated HPA response, whereas LAB rats show an elevated HPA response. In both rodent lines, the display of aggression is associated with high neuronal activation in the central amygdala, but reduced neuronal activation in the lateral septum. Furthermore, in the lateral septum, SAL mice have a reduced vasopressinergic fiber network, and LAB rats show a decreased vasopressin release during the display of aggression. Moreover, the two lines show several indications of an increased serotonergic neurotransmission. The relevance of these findings in relation to high aggression and stress coping is discussed. In conclusion, exploring neurobiological systems in animals sharing relevant behavioral characteristics might be a useful approach to identify general mechanisms of action, which in turn can improve our understanding of specific behavioral symptoms in human psychiatric disorders.
Folksonomies - Indexing and Retrieval in Web 2.0

Collaborative information services on Web 2.0 are used by Internet users not only to produce digital information resources, but also to furnish the contents of the resources with their own keywords, so-called tags. Whilst doing so the user is not required to comply with rules, as is necessary with a library catalogue. The amount of user-generated tags in a collaborative information service is referred to as folksonomy. Folksonomies allow users to relocate their own resources and to search for other resources. This book deals with collaborative information services and folksonomies both as a method of representing knowledge and a tool for information retrieval.
The position of the arm during blood pressure measurement in sitting position

ObjectiveDetermining the influence of the position of the arm on blood pressure measurement in the sitting position. MethodsBlood pressure of 128 individuals (the majority being treated hypertensive patients) visiting the outpatient clinic was measured simultaneously on both arms with arms in two different positions. First, both arms were placed at the chair support level and blood pressure was measured three times on both arms after 10 min of rest. Subsequently, while still remaining in the same sitting position, five blood pressure measurements were made simultaneously at both arms with one arm placed on the desk and one arm placed and supported at heart level (mid-sternal). The arm placed at heart level served as the reference arm. The choice of which arm was placed at desk level and which arm was placed at heart level was randomized. ResultsBoth at desk level and at chair support level, mean (±SD) systolic and diastolic blood pressures were higher than blood pressure at heart level by 6.1/5.7±4.6/3.1 and 9.3/9.4±5.4/3.4 mmHg, respectively. The effect of the height differences between the arm positions on the blood pressure readings was smaller than predicted (0.49 mmHg/cm systolic and 0.47 mmHg/cm diastolic). No significant correlation was found between blood pressure difference in the different arm positions (desk and heart level) and age, sex, weight or baseline blood pressure. ConclusionsDifferent arm positions below heart level have significant effects on blood pressure readings. The leading guidelines about arm position during blood pressure measurement are not in accordance with the arm position used in the Framingham study, the most frequently used study for risk estimations.
The role of services in homebuyers’ attitudes: A field experiment in the French off-plan housing market

This article investigates the effects of services proposed by private developers on buyers’ psychological attitudes in the decision-making process for off-plan purchases. We propose a new typology of services based on perceived value. We conducted a field experiment with real buyers who purchased an off-plan dwelling in France, and we measured the causal effects on developer reputation of four service intensity indexes that offer ‘visualisation’, ‘customisation’, ‘value enhancement’ and ‘interaction’ services. The results confirm the homebuyer-based typology of services and highlight the mediating roles of two types of attitudes, satisfaction with and trust in the developer, to explain the influence on developer reputation. Services designed to improve the perception of the quality of the dwelling itself (visualisation and customisation benefits) are less effective, and those intended to create a favourable purchase context (value enhancement and interactional benefits) are more influential, emphasising the benevolent role expected from developers.
Mechanism, Glasgow Coma Scale, Age, and Arterial Pressure (MGAP): A new simple prehospital triage score to predict mortality in trauma patients*

Objectives:Prehospital triage of trauma patients is of paramount importance because adequate trauma center referral improves survival. We developed a simple score that is easy to calculate in the prehospital phase. Design:Multicenter prospective observational study. Setting:Prehospital physician-staffed emergency system in university and nonuniversity hospitals. Interventions:We evaluated 1360 trauma patients receiving care from a prehospital mobile intensive care unit in 22 centers in France during 2002. The association of prehospital variables with in-hospital death was tested using logistic regression, and a simple score (the Mechanism, Glasgow coma scale, Age, and Arterial Pressure [MGAP] score) was created and compared with the triage Revised Trauma Score, Revised Trauma Score, and Trauma Related Injury Severity Score. The model was validated in 1003 patients from 2003 through 2005. Measurements and Main Results:Four independent variables were identified, and each was assigned a number of points proportional to its regression coefficient to provide the MGAP score: Glasgow Coma Scale (from 3–15 points), blunt trauma (4 points), systolic arterial blood pressure (>120 mm Hg: 5 points, 60 to 120 mm Hg: 3 points), and age <60 yrs (5 points). The area under the receiver operating characteristic curve of MGAP was not significantly different from that of the triage Revised Trauma Score or Revised Trauma Score, but when sensitivity was fixed >0.95 (undertriage of 0.05), the MGAP score was more specific and accurate than triage Revised Trauma Score and Revised Trauma Score, approaching those of Trauma Related Injury Severity Score. We defined three risk groups: low (23–29 points), intermediate (18–22 points), and high risk (<18 points). In the derivation cohort, the mortality was 2.8%, 15%, and 48%, respectively. Comparable characteristics of the MGAP score were observed in the validation cohort. Conclusion:The MGAP score can accurately predict in-hospital death in trauma patients.
Sense of coherence, coping and quality of life following a critical illness.

AIM This paper reports the findings of a study exploring the relationships among patients' sense of coherence, coping ability and health-related quality of life following a critical illness. BACKGROUND Sense of coherence is a strong determinant of positive health outcomes and successful coping. Promoting sense of coherence has been used for health promotion in the general public so that better coping ability will be achieved and, thus, better quality of life. There have been limited studies focusing on these aspects in critically ill patients, particularly during the weeks following discharge from intensive care units. METHODS Based on the salutogenic theoretical framework, a descriptive, correlational research design using telephone interviews was used. Participants' outlook on life, quality of life and coping responses were measured using Chinese versions of Sense of Coherence scale, SF-36 and Coping Scale, respectively. RESULTS Eighty-eight people participated in the study. Sense of coherence was significantly correlated with quality of life (r = 0.28-0.69) and with coping ability (r = 0.58). Furthermore, coping ability was significantly correlated with six (r = 0.25-0.52) out of the eight dimensions of quality of life. It was also found that high household income and support from adult children were predictors of strong sense of coherence. CONCLUSION Patients with a strong sense of coherence are likely to take an active role in shaping their own health outcomes. The results of this study will enable nurses to design interventions in the early phase of hospitalization to enhance patients' sense of coherence and coping abilities following a critical illness so that quality of life can be promoted.
Improved targeting of the alpha(v)beta (3) integrin by multimerisation of RGD peptides.

PURPOSE The integrin alpha(v)beta(3) is expressed on sprouting endothelial cells and on various tumour cell types. Due to the restricted expression of alpha(v)beta(3) in tumours, alpha(v)beta(3) is considered a suitable receptor for tumour targeting. In this study the alpha(v)beta(3) binding characteristics of an (111)In-labelled monomeric, dimeric and tetrameric RGD analogue were compared. METHODS A monomeric (E-c(RGDfK)), dimeric (E-[c(RGDfK)](2)), and tetrameric (E{E[c(RGDfK)](2)}(2)) RGD peptide were synthesised, conjugated with DOTA and radiolabelled with (111)In. In vitro alpha(v)beta(3) binding characteristics were determined in a competitive binding assay. In vivo alpha(v)beta(3) targeting characteristics of the compounds were assessed in mice with SK-RC-52 xenografts. RESULTS The IC(50) values for DOTA-E-c(RGDfK), DOTA-E-[c(RGDfK)](2), and DOTA-E{E[c(RGDfK)](2)}(2)were 120 nM, 69.9 nM and 19.6 nM, respectively. At all time points, the tumour uptake of the dimer was significantly higher as compared to that of the monomer. At 8 h p.i. , tumour uptake of the tetramer (7.40+/-1.12%ID/g) was significantly higher than that of the monomer (2.30+/-0.34%ID/g), p<0.001, and the dimer (5.17+/-1.22%ID/g), p<0.05. At 24 h p.i. , the tumour uptake was significantly higher for the tetramer (6.82+/-1.41%ID/g) than for the dimer (4.22+/-0.96%ID/g), p<0.01, and the monomer (1.90+/-0.29%ID/g), p<0.001. CONCLUSION Multimerisation of c(RGDfK) resulted in enhanced affinity for alpha(v)beta(3) as determined in vitro. Tumour uptake of a tetrameric RGD peptide was significantly higher than that of the monomeric and dimeric analogues, presumably owing to the enhanced statistical likelihood for rebinding to alpha(v)beta(3).