Miracle-dz/UniFAR-FTUs huggingface dataset:
SELF-PRUNING Acts Synergistically with DIAGEOTROPICA to Guide Auxin Responses and Proper Growth Form1

The antiflorigenic signal SELF-PRUNING controls tomato growth habit by affecting auxin transport, signaling, and metabolism. The SELF PRUNING (SP) gene is a key regulator of growth habit in tomato (Solanum lycopersicum). It is an ortholog of TERMINAL FLOWER1, a phosphatidylethanolamine-binding protein with antiflorigenic activity in Arabidopsis (Arabidopsis thaliana). A spontaneous loss-of-function mutation (sp) has been bred into several industrial tomato cultivars, as it produces a suite of pleiotropic effects that are favorable for mechanical harvesting, including determinate growth habit, short plant stature, and simultaneous fruit ripening. However, the physiological basis for these phenotypic differences has not been thoroughly explained. Here, we show that the sp mutation alters polar auxin transport as well as auxin responses, such as gravitropic curvature and elongation of excised hypocotyl segments. We also demonstrate that free auxin levels and auxin-regulated gene expression patterns are altered in sp mutants. Furthermore, diageotropica, a mutation in a gene encoding a cyclophilin A protein, appears to confer epistatic effects with sp. Our results indicate that SP affects the tomato growth habit at least in part by influencing auxin transport and responsiveness. These findings suggest potential novel targets that could be manipulated for controlling plant growth habit and improving productivity.
RSRN: Rich Side-Output Residual Network for Medial Axis Detection

In this paper, we propose a Rich Side-output Residual Network (RSRN) for medial axis detection for the ICCV 2017 workshop challenge on detecting symmetry in the wild. RSRN uses the rich features of fully convolutional network by hierarchically fusing side-outputs in a deep-to-shallow manner to decrease the residual between the detection result and the ground-truth, which refines the detection result hierarchically. Experimental results show that the proposed RSRN improves the performance compared with baseline on both SKLARGE and BMAX500 datasets.
Orthographic Properties Based Telugu Text Recognition Using Hidden Markov Models

Telugu script has the glyphs for vowels, consonants and modifiers to form orthographic units called aksharas (characters). In this paper, we present the Telugu printed text recognition based on orthographic properties of the script and Hidden Markov Models (HMMs). One of the orthographic properties is that the consonant modifiers always appear spatially in the middle and lower zones. The concept of peak fringe numbers (PFNs) is used to define a first level classifier. The purpose of the first level classification is to classify the Telugu word images based on the data in lower zone for better modeling. Since conventional Telugu OCR applications facing segmentation difficulties at various levels of segmentation such as akshara and connected-component. We use HMMs for modeling Telugu akshara shapes and also apply bi-grams of aksharas at the recognition stage. Our approach aims to overcome the segmentation problems by attempting a segmentation-free method for Telugu printed text recognition. With the suitability of HMMs for modeling data with variations, our data set also includes document images with different DPIs such as 200, 250 and 300. We measure character error rate (CER) to observe the system performance. The recognition capability of the system is encouraging and the CER of our system is 15 percent.
Endogenous opioidergic dysregulation of pain in fibromyalgia: a PET and fMRI study

Abstract Endogenous opioid system dysfunction potentially contributes to chronic pain in fibromyalgia (FM), but it is unknown if this dysfunction is related to established neurobiological markers of hyperalgesia. We previously reported that µ-opioid receptor (MOR) availability was reduced in patients with FM as compared with healthy controls in several pain-processing brain regions. In the present study, we compared pain-evoked functional magnetic resonance imaging with endogenous MOR binding and clinical pain ratings in female opioid-naive patients with FM (n = 18) using whole-brain analyses and regions of interest from our previous research. Within antinociceptive brain regions, including the dorsolateral prefrontal cortex (r = 0.81, P < 0.001) and multiple regions of the anterior cingulate cortex (all r > 0.67; all P < 0.02), reduced MOR availability was associated with decreased pain-evoked neural activity. Additionally, reduced MOR availability was associated with lower brain activation in the nucleus accumbens (r = 0.47, P = 0.050). In many of these regions, pain-evoked activity and MOR binding potential were also associated with lower clinical affective pain ratings. These findings are the first to link endogenous opioid system tone to regional pain-evoked brain activity in a clinical pain population. Our data suggest that dysregulation of the endogenous opioid system in FM could lead to less excitation in antinociceptive brain regions by incoming noxious stimulation, resulting in the hyperalgesia and allodynia commonly observed in this population. We propose a conceptual model of affective pain dysregulation in FM.
Enhancement of erythritol production by Trichosporonoides oedocephalis ATCC 16958 through regulating key enzyme activity and the NADPH/NADP ratio with metal ion supplementation

ABSTRACT Erythritol, a well-known natural sweetener, is mainly produced by microbial fermentation. Various metal ions (Al3+, Cu2+, Mn2+, and Ni2+) were added to the culture medium of Trichosporonoides oedocephalis ATCC 16958 at 30 mg/L in shake flask cultures. Compared with controls, Cu2+ increased the erythritol content by 86% and decreased the glycerol by-product by 31%. After 48 hr of shake flask culture, sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that expression levels of erythrose reductase (ER) in the presence of 30 mg/L CuSO4 · 5H2O were higher than those obtained after treatment with other examined metal ions. Furthermore, after 108 hr of batch culture in a 5-L bioreactor, supplementation with 30 mg/L of CuSO4 · 5H2O increased the specific erythritol content by 27%. Further studies demonstrated that ER activity under 30 mg/L CuSO4 · 5H2O supplementation in a fermentor was overtly increased compared with the control after 60 hr, while glycerol-3-phosphate dehydrogenase activity was clearly reduced in most of the fermentation process. Furthermore, the NADPH/NADP ratio was slightly lower in T. oedocephalis cells treated with Cu2+ compared with control cells. These results provide further insights into Cu2+ effects on erythritol biosynthesis in T. oedocephalis and should improve the industrial production of erythritol by biological processes.
Nup358 binds to AGO proteins through its SUMO‐interacting motifs and promotes the association of target mRNA with miRISC

MicroRNA (miRNA)‐guided mRNA repression, mediated by the miRNA‐induced silencing complex (miRISC), is an important component of post‐transcriptional gene silencing. However, how miRISC identifies the target mRNA in vivo is not well understood. Here, we show that the nucleoporin Nup358 plays an important role in this process. Nup358 localizes to the nuclear pore complex and to the cytoplasmic annulate lamellae (AL), and these structures dynamically associate with two mRNP granules: processing bodies (P bodies) and stress granules (SGs). Nup358 depletion disrupts P bodies and concomitantly impairs the miRNA pathway. Furthermore, Nup358 interacts with AGO and GW182 proteins and promotes the association of target mRNA with miRISC. A well‐characterized SUMO‐interacting motif (SIM) in Nup358 is sufficient for Nup358 to directly bind to AGO proteins. Moreover, AGO and PIWI proteins interact with SIMs derived from other SUMO‐binding proteins. Our study indicates that Nup358–AGO interaction is important for miRNA‐mediated gene silencing and identifies SIM as a new interacting motif for the AGO family of proteins. The findings also support a model wherein the coupling of miRISC with the target mRNA could occur at AL, specialized domains within the ER, and at the nuclear envelope.
Investigation of relationship of the mitochondrial DNA 16189 T>C polymorphism with metabolic syndrome and its associated clinical parameters in Turkish patients

OBJECTIVEMitochondrial DNA (mtDNA) polymorphisms have been implicated in the pathophysiology of human diseases. Among them, a T>C nucleotide transition on the 16189 nucleotide position of mtDNA has been studied in several metabolic diseases including diabetes and obesity. In this study we aimed to investigate the association of this polymorphism among Turkish metabolic syndrome patients.DESIGNA total of 220 cases (70 MetS patients and 150 healthy control subjects) were evaluated for their mtDNA 16189 variant by PCR-RFLP technique. In addition, clinical and biochemical variables, such as cholesterol levels, body fat percentage, insulin resistance and presence of type II diabetes, were also evaluated.RESULTSOverall frequency of polymorphic C allele was determined as 0.19 without a significant association with type II diabetes and metabolic syndrome. This may be partly due to ethnical differences of populations studied and may also be related to other genetic and environmental factors. Moreover, there were no significant associations with biochemical variables among metabolic syndrome patients, except LDL and suppressed cortisol (sup-cortisol) levels. Low levels of LDL and sup-cortisol were significantly associated with the mtDNA 16189 variant, though the biochemical mechanism underlying this effect is not clear.CONCLUSIONSThis is the first study involving a Turkish population on the mtDNA 16189 T>C polymorphism. Further studies with larger cohorts will be needed to elucidate its relation with metabolic syndrome as well as lipid metabolism.
The Trouble with Security Requirements

Manifold approaches to security requirements engineering have been proposed, yet there is no consensus how to elicit, analyze, or express security needs. This perspective paper systematizes the problem space of security requirements engineering. Security needs result from the interplay of three dimensions: threats, security goals, and system design. Elementary statements can be made in each dimension, but such one-dimensional requirements remain partial and insufficient. To understand security needs, one has to analyze their interaction. Distinct analysis tasks arise for each pair of dimensions and are supported by different techniques: risk analysis, as in CORAS, between threats and security goals; security design, as exemplified by the framework of Haley et al., between goals and design; and security design analysis, such as Microsoft's threat modeling technique with data flow diagrams and STRIDE, between design and threats. All three perspectives are necessary to develop secure systems. Security requirements engineering must iterate through them, because threats determine the relevance of security goals, security design seeks ways to fulfill them, and design choices themselves influence threats and security goals.
Myrmecridium hiemale sp. nov. from snow-covered alpine soil is the first eurypsychrophile in this genus of anamorphic fungi.

Myrmecridium hiemale sp. nov. was isolated from snow-covered alpine bare soil and is described as the first eurypsychrophilic species of this genus of filamentous fungi. Colony growth temperature experiments were carried out in the range 4-37 °C. Morphological characteristics and colony appearance were in accordance with characteristics typical for Myrmecridium, but M. hiemale does not grow at temperatures of 25 °C and above. Sequence analyses of the internal transcribed spacer and LSU rRNA D1/D2 regions indicated that the strain in question represents a distinct taxon within the genus Myrmecridium (Myrmecridiaceae, Sordariomycetes, Ascomycota). The type strain of M. hiemale is CBS 141017T(=JMRC 12083T). A morphological description is provided, and a key is presented for the currently known taxa of Myrmecridium, a group of interesting fungi that are either saprobes or plant endophytes.
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Microbes provide an intriguing system to study social interaction among individuals within a population. The short generation times and relatively simple genetic modification procedures of microbes facilitate the development of the sociomicrobiology field. To assess the fitness of certain microbial species, selected strains or their genetically modified derivatives within one population, can be fluorescently labelled and tracked using microscopy adapted with appropriate fluorescence filters. Expanding colonies of diverse microbial species on agar media can be used to monitor the spatial distribution of cells producing distinctive fluorescent proteins. Here, we present a detailed protocol for the use of green- and red-fluorescent protein producing bacterial strains to follow spatial arrangement during surface colonization, including flagellum-driven community movement (swarming), exopolysaccharide- and hydrophobin-dependent growth mediated spreading (sliding), and complex colony biofilm formation. Non-domesticated isolates of the Gram-positive bacterium, Bacillus subtilis can be utilized to scrutinize certain surface spreading traits and their effect on two-dimensional distribution on the agar-solidified medium. By altering the number of cells used to initiate colony biofilms, the assortment levels can be varied on a continuous scale. Time-lapse fluorescent microscopy can be used to witness the interaction between different phenotypes and genotypes at a certain assortment level and to determine the relative success of either.
Alfalfa and Pastures: Sources of Pests or Generalist Natural Enemies?

Abstract Pierce's disease of grapevine and almond leaf scorch disease are both caused by the bacterial pathogen Xylella fastidiosa Wells et al. In the Central Valley of California, Draeculacephala minerva Ball (Hemiptera: Cicadellidae) is the most common vector of X. fastidiosa. As alfalfa fields and pastures are considered source habitats for D. minerva, it is recommended that almond orchards and vineyards should be distanced from alfalfa and pastures. Here, risk of alfalfa and pastures serving as sources of D. minerva was compared to the potential benefit of alfalfa and pastures serving as sources of generalist natural enemies belonging to the families Chrysopidae and Coccinellidae. Populations of D. minerva were greatest in pastures, whereas chrysopids were least abundant in pastures, and coccinellids were only moderately more abundant in pastures than in vineyards or almond orchards. Accordingly, risk of pastures serving as a source of D. minerva was not offset by any potential benefit of pastures serving as a source of chrysopids or coccinellids. Abundance of D. minerva in alfalfa was low, whereas abundance of chrysopids and coccinellids in alfalfa was high. Thus, well-maintained alfalfa fields were a minor source of D. minerva that may contribute chrysopids and coccinellids to surrounding habitats. Spissistilus festinus (Say) (Hemiptera: Membracidae), a recently identified vector of grapevine red blotch virus, was abundant in alfalfa fields and was observed in vineyards. Thus, a full evaluation of the risk of planting vineyards near alfalfa may require considering risk associated with movement of S. festinus.
Effect of Educational Program, Based on PRECEDE and Trans-Theoretical Models, on Preventing Decline in Regular Physical Activity and Improving it among Students

Background: Adolescents especially middle schoolgirls do not follow 60 min of regular physical activity (RPA), recommended by WHO (World Health Organization), and endure physical activity decline. Using theory-based interventional program, considering the age of decline in RPA, seems to be effective. The aim of this study was to determine the effects of educational program, based on PRECEDE and Trans-Theoretical models, on preventing decline in RPA and improving it among 7th grade girl students. Study design: Randomized controlled trial. Methods: This study was conducted on 7th grade girl students in Hamadan, west of Iran from 2015- 16. Participants were divided into intervention (N=179) and control (N=165) groups by random assignment. Physical activity questionnaire for adolescents and the model-based questionnaire were used before and after intervention. Intervention included two months education and six months follow up. The effects of intervention were determined by statistical test and analysis of covariance using SPSS version 16. Results: The higher change in the mean scores was observed in self-efficacy (0.86), counter condition (0.66) and helping relationship (0.57) in the intervention group (P≤0.001). In addition, a significant difference (P ≤ 0.01) was observed between the two groups 6 months after the intervention in all constructs of model. The mean score of doing physical activity in the intervention group increased from 2.50 to 3.17 that it was differed significantly from the control group (P≤0.001). Conclusions: School based intervention using PRECEDE and Trans-Theoretical models might prevent girl students’ RPA decline and improve their RPA.
The MainStreetXpress Core Services Node - A Versatile ATM Switch Architecture for the Full Service Network

Switching systems based on the ATM principle have outgrown the experimental stage, and are already used today in private and corporate networks, as well as in public wide area networks to provide regular service. ATM has the inherent ability to provide a common basis for transmission and switching functionality in both local and wide area networks. With the potential to support all services available today as well as those envisaged for the future, ATM holds a strong promise for network operators and end customers. To fully exploit this potential, ATM switch architectures are required which provide versatility and modularity in supporting services and protocols, independent scalability of data throughput and control performance over a wide range, and also reliability features adaptable to the respective application scenario. This paper describes in some detail how the MainStreetXpress core services node, which has evolved from the prototype described by Fischer et. al. (1991) to a mature central office ATM switch, addresses these issues and provides a future-proof architecture incorporating all of the features required in the B-ISDN era.
Implementing health and social care integration in Scotland: Renegotiating new partnerships in changing cultures of care

Health and social care integration has been a long-term goal for successive governments in Scotland, culminating in the implementation of the recent Public Bodies (Joint Working) Scotland Act 2014. This laid down the foundations for the delegation of health and social care functions and resources to newly formed Integrated Joint Boards. It put in place demands for new ways of working and partnership planning. In this article, we explore the early implementation of this Act and how health and social care professionals and the third sector have begun to renegotiate their roles. The paper draws on new empirical data collated through focus groups and interviews with over 70 professionals from across Scotland. The data are explored through the following key themes: changing cultures, structural imbalance, governance and partnership and the role of individuals or "boundary spanners" in implementing change. We also draw on evidence from other international systems of care, which have implemented integration policies, documenting what works and what does not. We argue that under the current framework much of the potential for integration is not being fulfilled and that the evidence suggests that at this early stage of roll-out, the structural and cultural policy changes that are required to enable this policy shift have not yet emerged. Rather, integration has been left to individual innovators or "boundary spanners" and these are acting as key drivers of change. Where change is occurring, this is happening despite the system. As it is currently structured, we argue that too much power is in the hands of health and despite the rhetoric of partnership working, there are real structural imbalances that need to be reconciled.
Performance on Balance Evaluation Systems Test (BESTest) Impacts Health-Related Quality of Life in Adult Spinal Deformity Patients

Study Design. Prospective single-center study. Objective. Study investigates how dynamic balance performance complements 2D static radiographic measurements and demographics in terms of understanding health-related quality of life in adult spinal deformity (ASD) patients. Summary of Background Data. Recent insights suggest that demographic variables have a stronger impact on health-related quality of life than 2D radiographic spinopelvic parameters in ASD patients. Methods. Nine healthy volunteers and 36 ASD patients following inclusion criteria were recruited. Demographics, Scoliosis Research Society Score-22r (SRS-22r), Oswestry Disability Index (ODI), Core Outcome Measures Index (COMI), 2D radiographic spinopelvic measurements, and performance on Balance Evaluation Systems Test (BESTest), and Trunk Control Measurement Scale (TCMS) were determined for each subject. Nonparametric tests, Spearman correlations, univariate, and stepwise-like linear multivariate regression analysis were performed. Results. BESTest and TCMS had significant lower values in the ASD group versus the control group (P = 0.000). In the ASD group, Cumulative Illness Rating Scale (CIRS) correlated fair to ODI, COMI (0.441 ≥ r ≥ 0.383, P < 0.021) and to SRS-22-r (r = −0.335, P = 0.046), Mini Mental State Examination correlated fair to COMI (r = −0.352, P = 0.035), “Pelvic Incidence minus Lumbar Lordosis” correlated fair to ODI (r = 0.361, P = 0.031), BESTest correlated moderate to ODI and COMI (r ⩽ −0.505; P ⩽ 0.002), TCMS correlated fair to ODI (r = −0.356; P = 0.033). CIRS and BESTest were significant predictive variables for COMI based on univariate analysis in ASD patients. Multivariate regression analysis including demographics, 2D static radiographic parameters, and dynamic balance scales identified BESTest as single independent variable (P = 0.000) to predict COMI (adjusted R2 = 0.285) in ASD patients. Conclusion. BESTest has a higher potential than demographic and 2D radiographic spinopelvic parameters to predict quality of life in ASD patients. Further research is necessary to identify the impact of ASD on quality of life. Level of Evidence: 3
The Lifecycle Growth Trend and Demand Prediction of China Copper Industry

With the rapid economic development in China, the domestic copper demand grows sustainedly and drives the continual expansion of China copper industry. The study is based on the industry lifecycle theory and supported by the lifecycle characteristics of non-ferrous metal industry of the current developed countries. Combined the experienced data of early developed industrial countries to our practical situation, the growth trend and development limitation of China copper industry lifecycle are analyzed by the growth curve model, which is significant for the accord development of China copper industry.