Miracle-dz/UniFAR-FTUs huggingface dataset:
Trag- und Verformungsverhalten von Rahmenknoten

Ziel des Forschungsvorhabens war es, die Grenzen der Rotationsfaehigkeit bei Stabtragwerken im Bereich von Rahmenknoten unter Beruecksichtigung baupraktischer Gegebenheiten abzuklaeren, um so die Anwendbarkeit des Traglastverfahrens zu pruefen. Die Grenzen der plastischen Verformbarkeit waren experimentell zu ueberpruefen. Bei frueher durchgefuehrten Versuchen wurde als Bewehrungsstahl fast ausschliesslich Betonstahl 420/500 verwendet. Der Verformungsfaehigkeit und dem Verhalten unter Gebrauchslast wurden zu wenig Aufmerksamkeit entgegengebracht. In neuen Versuchen war daher die Verformungsfaehigkeit von Rahmenecken und Rahmenknoten bei Verwendung von Betonstahl 500/550 festzustellen, und es sollte durch Messung von Rissbreiten und Rissabstaenden sowie der Verformungen (Winkelaenderungen) unter verschiedenen Laststufen beurteilt werden, ob auch ein einwandfreies Verhalten der Bauteile im Gebrauchszustand gegeben ist. Wegen zu knapper Haushaltsmittel konnten nur wenige ausgewaehlte Versuche durchgefuehrt werden, deshalb, und wegen des zunehmenden Einsatzes von hochfestem Beton, zum Beispiel bei Hochhaeusern mit tragenden Skelettkonstruktionen, bleiben noch offene Fragen, die in weiteren Versuchen geklaert werden sollten. Rahmenecken unter Einwirkung positiver Momente und Rahmenendknoten zeigten sich schon im Gebrauchslastbereich erheblich verformungsweicher als die anschliessenden Riegel beziehungsweise Stuetzen, so dass Umlagerungen der Schnittgroessen bei selbststabilisierenden Rahmentragwerken grundsaetzlich zu verbieten sind. Von einer Anwendung des Traglastverfahrens wird bei der Bemessung von Rahmenecken und Rahmenknoten in verschieblichen Rahmensystemen unbedingt abgeraten. Das Arbeitsprogramm, die Durchfuehrung der Versuche und Bewehrungsvorschlaege werden eingehend dargelegt. Forschungsprojekt, gefoerdert von der Deutschen Forschungsgemeinschaft (DFG - Ko 204/37).
A phenomenological perspective on advanced practice nurse–physician collaboration within an interdisciplinary healthcare team

Purpose: To investigate how advanced practice nurses (APNs) and physicians (MDs) within a multisite nursing home practice perceive and describe their experiences of collaboration in an interdisciplinary team. Data sources: A phenomenological analysis of in‐depth interviews of eight APNs and five MDs working in team practice across multiple nursing homes. Conclusions: This phenomenological analysis reveals that the lived experience of these professionals affirms four thematic clusters (meaning units) as essential for collaboration: approachability, interpersonal skills, listening, and verbal message skills. However, APNs and MDs diverge in their perceptions of the behaviors currently operative or required to achieve the collaboration components sought. The invariants—that without which a phenomenon cannot be—differ for APNs and MDs. Implications for practice: Although both parties reference identical terms, when the language is unpacked, different behaviors are sought by APNs and MDs to achieve collaboration. This suggests that discussions concerning collaboration between APNs and MDs should not remain at the level of generalizations wherein apparent agreement might be assumed; instead, focused exchanges must concern specific behaviors in discrete instances.
Validation of the THI-12 questionnaire for international use in assessing tinnitus: A multi-centre, prospective, observational study

Abstract Objective: To investigate and confirm the reliability and validity of the tinnitus handicap inventory12 (THI-12) in various countries and languages. Design: Prospective, observational study conducted in seven countries, using linguistically harmonized versions of the THI-12 in six languages. These were evaluated for test-retest reliability, internal consistency reliability, known-groups validity, and construct validity. Basic psychometric properties of supporting instruments were compared. Questionnaires were completed by the subjects at baseline and again after 12–30 days. Study sample: Adults with a clinical diagnosis of subjective tinnitus. Results: An exploratory factor analysis of the THI-12 items for the U.S. study population at baseline revealed a single common factor of high eigenvalue. Confirmatory factor analysis supported this in the separate countries. Test-retest reliability was moderate to high, and the conclusions were supported by a known-groups analysis; correlations with other scales expected to support construct validity were moderate. Conclusions: The THI-12 total score showed acceptable psychometric properties for all countries tested. The relationships between the THI-12 and the one-month and one-week versions of the TRS and TSS were similar and convergent. The THI-12 is thus a promising diagnostic tool for assessing treatment effects in multi-cultural and multi-lingual trials on tinnitus therapy.
Production of Humanized Antibody against Human High-affinity IgE Receptor in a Serum-free Culture of CHO Cells, and Purification of the Fab Fragments

We describe the preparation of Fab fragments of a humanized anti-human high-affinity IgE receptor (FcεRIα) antibody potentially useful for treatment of IgE-mediated allergic diseases. IgE-binding capacities of sixteen combinations of light and heavy chains of four recombinant anti-FcεRIα antibodies, chimeric CRA2, humanized CRA2, chimeric CRA4, and humanized CRA4, were compared. A combination in which both chains were of humanized CRA2 had the highest activity. Stable transfectant clones of four kinds of host cells expressing recombinant antibodies were established. CHO-K1 cells were the most productive. Serum-free media suitable for culture of the stable CHO-transfectant clones were screened. The concentration of the humanized CRA2, which the most productive clone secreted into the chosen serum-free medium, was approximately 100 μg/ml. A procedure for the purification of the antibody, papain-digestion, and purification of Fab fragments was established. The highly purified humanized Fab fragments are suitable for use to examine their in vivo activity and immunogenicity in primates.
A Comparison of Highly Instrumented and Minimally Instrumented Unicompartmental Knee Prostheses

In this study, two specific implants are compared: the Oxford prosthesis, which uses a highly instrumented technique and was placed through a traditional arthrotomy; and the Repicci prosthesis, which uses a minimally instrumented technique and was placed with a minimally invasive surgical approach. The study looked specifically at limb alignment, because achieving appropriate alignment is correlated with the best long-term implant function and achieving alignment is a function of the implant instrumentation. The rationale of more instrumentation for a surgical technique is to add precision and reproducibility; less instrumentation requires less surgical exposure and therefore is more minimally invasive. The senior author presents his long-term experience with the Oxford implant (55 implants; average followup 10.4 years) and short-term experience with the Repicci implant (164 implants; average followup 1.3 years for the first 30 implants.). With available followup, the clinical results using both implants have been favorable. The average alignment of the Oxford prosthesis was 5.6° valgus and the average alignment of the Repicci prosthesis was 4.5° valgus. Side-to-side long-term comparison is not yet available, although the literature would suggest that both implants can have satisfactory long-term results. Specific implant selection probably is not as important as precise patient selection.
Safety and efficacy of oxaliplatin and fluoropyrimidine regimens with or without bevacizumab as first-line treatment of metastatic colorectal cancer: results of the TREE Study.

PURPOSE To evaluate the safety and efficacy of three oxaliplatin and fluoropyrimidine regimens, with or without bevacizumab, as first-line treatment for metastatic colorectal cancer (CRC). PATIENTS AND METHODS Patients with histologically documented metastatic or recurrent CRC and no prior treatment for advanced disease were randomly assigned to mFOLFOX6 (bolus and infusion fluorouracil [FU] and leucovorin [LV] with oxaliplatin), bFOL (bolus FU and low-dose LV with oxaliplatin), or CapeOx (capecitabine with oxaliplatin), respectively (Three Regimens of Eloxatin Evaluation [TREE-1]). The study was later modified such that subsequent patients were randomized to the same regimens plus bevacizumab (TREE-2). RESULTS A total of 150 and 223 patients were randomly assigned in the TREE-1 and TREE-2 cohorts, respectively. Incidence of grade 3/4 treatment-related adverse events during the first 12 weeks of treatment were 59%, 36%, and 67% for mFOLFOX6, bFOL, and CapeOx, respectively, (TREE-1) and 59%, 51%, and 56% for the corresponding treatments plus bevacizumab (TREE-2; primary end point). CapeOx toxicity in TREE-1 included grade 3/4 diarrhea (31%) and dehydration (27%); capecitabine dose reduction to 1,700 mg/m(2)/d in TREE-2 resulted in improved tolerance. Overall response rates were 41%, 20%, and 27% (TREE-1) and 52%, 39%, and 46% (TREE-2); median overall survival (OS) was 19.2, 17.9, and 17.2 months (TREE-1) and 26.1, 20.4, and 24.6 months (TREE-2). For all treated patients, median OS was 18.2 months (95% CI, 14.5 to 21.6; TREE-1) and 23.7 months (95% CI, 21.3 to 26.8; TREE-2). CONCLUSION The addition of bevacizumab to oxaliplatin and fluoropyrimidine regimens is well tolerated as first-line treatment of mCRC and does not markedly change overall toxicity. CapeOx tolerability and efficacy is improved with reduced-dose capecitabine. First-line oxaliplatin and fluoropyrimidine-based therapy plus bevacizumab resulted in a median OS of approximately 2 years.
Depletion of CD4(+) T cells aggravates glomerular and interstitial injury in murine adriamycin nephropathy.

BACKGROUND CD4(+) T cells play an important role in various types of immunologic renal disease, including lupus nephritis, IgA nephropathy, and crescentic glomerulonephritis. CD4(+) T cells are also major infiltrating lymphocytes in chronic tubulointerstitial inflammation associated with nonimmunological renal diseases. We suspected that CD4(+) T cells might contribute to disease progression and loss of renal function in chronic proteinuric renal disease (CPRD). To investigate this possibility, the effect of monoclonal antibody against CD4(+) lymphocytes (anti-CD4) was studied in a murine model (adriamycin nephropathy) of CPRD. METHODS Adriamycin nephropathy was produced in male BALB/c mice by a single intravenous injection of adriamycin (11 mg/kg). Anti-CD4 was given by intraperitoneal injection following the development of proteinuria at days 5, 6, 7, 21, and 37 after adriamycin. After six weeks, renal function and histology were studied by histomorphometry, immunohistochemistry, and flow cytometry. RESULTS Flow cytometric analysis showed a marked decrease in the number of CD4(+) T cells in blood and spleen of the antibody-treated animals (N = 7, P < 0.01). Adriamycin plus CD4(+) depletion mice had significantly greater mesangial expansion, glomerular sclerosis, and interstitial expansion than the mice on adriamycin alone. Interstitial infiltration with macrophages and CD8(+) cells was significantly increased in adriamycin plus CD4(+) depletion mice. Creatinine clearance (17.5 +/- 0.54 vs. 29.2 +/- 0.89 microL/min, P < 0.001) was significantly worse in the adriamycin plus CD4(+) depletion mice than in adriamycin alone mice and correlated with histologic change in glomeruli and interstitium. CONCLUSIONS Depletion of CD4(+) T cells promotes glomerular and interstitial injury in mice with established adriamycin nephropathy. These findings suggest that CD4(+) T cells have a protective role against the progression of adriamycin nephropathy.
Evaluation of a transcondylar toggle system for stabilization of the cranial cruciate deficient stifle in small dogs and cats.

OBJECTIVE To evaluate use of a transcondylar toggle system (TCTS) for stabilization of the cranial cruciate ligament (CrCL) deficient stifle in small dogs and cats. STUDY DESIGN Prospective clinical study. ANIMALS Small dogs (<7 kg; n=14) and cats (2) with CrCL-associated lameness of <3 months duration and a tibial plateau angle <32 degrees. METHODS Affected animals had an extracapsular CrCL repair using the TCTS. Lameness score, muscle atrophy, osteoarthritis (OA) score, and range of motion (ROM) were evaluated preoperatively, and at 6 weeks and 7-10 months postoperatively. RESULTS Operative time was 75 +/- 16 minutes. Fifty-six percent required >1 bone tunnel attempts. One dog required revision at 2 weeks because of suture loosening. All stifles were stable at 6 weeks postoperatively. Fifteen animals were available for follow-up (7-10 months). Lameness improved significantly at 6 weeks (P<.0001), whereas muscle atrophy was worse at 6 weeks (P=.008) but improved at 7-10 months (P<.0001). OA scores were unchanged at 6 weeks (P=.08) but were significantly worse at 7-10 months (P<.0001). ROM remained unchanged at 6 weeks (P=1) and 7-10 months (P=.6). CONCLUSIONS The medially placed toggle provides a reliable short-term proximal anchor for the extracapsular suture with outcomes similar to other extracapsular techniques. The aiming device and drill bit are not recommended in their current form. CLINICAL RELEVANCE The TCTS appears to be a well-tolerated technique for proximal suture anchoring in extracapsular CrCL repair in small dogs and cats where instrumentation and anatomic constraints preclude other techniques.
Herpes simplex virus immediate-early promoters are responsive to virus and cell trans-acting factors

The promoters for each of the immediate-early genes from herpes simplex virus type 1 were cloned and fused to a chloramphenicol acetyltransferase cassette. These chimeric genes were used as targets in a transient expression assay to determine how the immediate-early gene products ICP4 and ICP0 and the virion-associated stimulatory protein Vmw65 affected their expression in HeLa and Vero cells. The basal level of expression from these cassettes differed significantly depending on the extent of 5'-flanking sequence and the cell line that served as host. The promoters from IE-4 and IE-0 behaved in a qualitatively similar fashion independent of the host cell. However, the promoter for ICP27 had a unique response pattern: in Vero cells it acted as an alpha gene promoter, whereas in HeLa cells its response was more like that of a beta gene promoter. The promoter sequences for ICP22 and ICP47 behaved as the IE-4 and IE-0 promoters did in HeLa cells, but their response to the effector molecules in Vero cells was unlike that of other alpha gene promoters we have studied. Evidence is also presented for a role for ICP27 in autoregulation.
Context-Dependent Modulation of Replication Activity of Saccharomyces cerevisiae Autonomously Replicating Sequences by Transcription Factors

ABSTRACT Evidence for transcription factor involvement in the initiation of DNA replication at certain replication origins in Saccharomyces cerevisiae mainly comes from an indirect assay which measures the mitotic stability of plasmids containing an autonomously replicating sequence (ARS), a selectable marker gene, and a centromere. In order to eliminate the effect of transcription factor binding to the selectable marker gene or centromere in such assays, we have adapted theDpnI assay to directly measure ARS replication activity in vivo by using ARS plasmids devoid of extraneous transcription elements. Using this assay, we found that the B3 element of ARS1, which serves as a binding site for the transcription factor Abf1p, does not stimulate ARS activity on plasmids lacking a centromere and a selectable marker gene. We also found with such plasmids that exogenous expression of the strong transcriptional activators Gal4 and Gal4-VP16 inhibited the replication activity of ARS1 when B3 was replaced by the Gal4 binding site, although these activators had previously been shown to stimulate replication activity in the stability assay. Moreover, a chromosomally inactive ARS, ARS301, which was active by itself on a plasmid, was inactivated by placing an Abf1p binding site in its vicinity. These results indicate that the sequences surrounding the ARS as well as properties of the ARS element itself determine its response to transcription factors.
Brill's companion to ancient Macedon : studies in the archaeology and history of Macedon, 650 BC-300 AD

Contributors Polyxeni Adam-Veleni, Ioannis M. Akamatis, Stella Drougou, Miltiades B. Hatzopoulos, Georgia Karamitrou-Mentessidi, Angeliki Kottaridi, Chaiido Koukouli-Chrysanthaki, Sophia Kremydi, Dimitris J. Kyrtatas, Robin Lane Fox, Louisa D. Loukopoulou, John Ma, Manuela Mari, Olga Palagia, Selene Psoma, Chryssoula Saatsoglou-Paliadeli, Stavros Paspalas, Thea Stefanidou-Tiveriou, Bettina Tsigarida
Association Analysis of Arsenic-Induced Straighthead in Rice (Oryza sativa L.) Based on the Selected Population with a Modified Model

A rice physiological disorder makes mature panicle keep erect with empty grains termed as “straighthead. ” Straighthead causes yield losses and is a serious threat to rice production worldwide. Here, a new study of association mapping was conducted to identify QTL involved in straighthead. A subset of 380 accessions was selected from the USDA rice core collection and genotyped with 72 genome-wide SSR markers. An optimal model implemented with principle components (PCs) was used in this association mapping. As a result, five markers were identified to be significantly associated with straighthead. Three of them, RM263, RM169, and RM224, were consistent with a previous study. Three markers, RM475, RM263, and RM19, had a resistant allele associated with a decrease in straighthead rating (straighthead rating ≤ 4.8). In contrast, the two other marker loci RM169 and RM224 had a few susceptible alleles associated with an increase in straighthead rating (straighthead rating ≥ 8.7). Interestingly, RM475 is close to QTL “qSH-2” and “AsS” with straighthead resistance, which was reported in two studies on linkage mapping of straighthead. This finding adds to previous work and is useful for further genetic study of straighthead.
Characterization of Growth Hormone Binding Sites in Granulosa and Theca Layers at Different Stages of Follicular Maturation and Ovulatory Cycle in the Domestic Hen

Abstract The currently available evidence points to a possible influence of growth hormone (GH) on avian folliculogenesis, which can be mediated by both hepatic- and ovarian-derived IGF-I. Therefore, the purpose of the present study was to reveal GH-binding sites in granulosa and theca layers of preovulatory follicles and to determine the binding characteristics depending on the degree of follicular maturation and the stage of the ovulatory cycle in the hen. Hens were killed 2 h (stage I), 9 h (stage II), 16 h (stage III), and 23 h (stage IV) after oviposition, and the five largest yellow follicles (from F1 to F5) were removed. GH-binding sites in granulosa and theca layers from F1 to F5 follicles were characterized using a radioreceptor assay. Equilibrium dissociation constants (Kd) and binding capacities (Bmax) were determined by Scatchard analysis of saturation curves, which revealed a single class of high-affinity GH-binding sites in both theca tissue and granulosa cells. In F1, F2, and F5 follicles, Bmax and Kd for GH-binding sites in the granulosa layer changed during the ovulatory cycle, decreasing between stages I and III, to increase again at stage IV, with alterations in Kd being less profound. No significant differences in binding capacities and affinities of GH-binding sites in the theca layer were found between various stages of the cycle. Furthermore, the concentration of GH-binding sites in the granulosa layer rose, whereas that in the theca layer fell with follicular enlargement. These findings indicate the presence of high-affinity GH-binding sites in both granulosa and theca layers of hen preovulatory follicles. Data also demonstrate that GH-binding sites in these tissues are regulated in a tissue-specific manner. Furthermore, the regulation of binding capacity of GH binding in granulosa cells by hormonal factors associated with ovulatory cycle is apparently not dependent on the state of follicular maturation.
Bifunctional sucrose phosphate synthase/phosphatase is involved in the sucrose biosynthesis by Methylobacillus flagellatus KT.

The aerobic obligate methylotroph Methylobacillus flagellatus KT was shown to synthesize sucrose in the presence of 0.5-2% NaCl in the growth medium. In the genome of this bacterium, an open reading frame (ORF) encoding a predicted 84-kD polypeptide homologous to the plant and cyanobacterial sucrose phosphate synthases (SPSs) was found. Using heterologous expression of the putative sps gene in Escherichia coli, followed by affinity chromatography, pure recombinant protein SPS-His6 was obtained. The enzyme catalyzed two reactions: conversion of fructose 6-phosphate and UDP-glucose into sucrose 6-phosphate and hydrolysis of sucrose 6-phosphate to sucrose. The bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP) was a 340 kDa homotetrameric Mg(2+) -dependent enzyme activated by fructose 1,6-phosphate2 and ATP but inhibited by glucose 6-phosphate, fructose 1-phosphate, AMP and inorganic phosphate. The amino acid sequence of the protein had a C-terminal domain homologous to SPPs. This correlated with the absence of the spp gene in the M. flagellatus chromosome. The ORFs homologous to the M. flagellatus SPS were found in the genomes of another obligate methylotroph Methylovorus glucosetrophus as well as the lithoautotrophic bacteria Acidithiobacillus ferrooxidans, Nitrosomonas europaea and Nitrosospira multiformis whose genomes lacked the spp genes. Thus, data extending the knowledge of biochemical properties of bacterial SPSs have been obtained.
A Brief Motivational Intervention for Treatment‐Refusing OCD Patients

Cognitive behavioral therapy involving exposure and response prevention (ERP) is the psychosocial treatment of choice for obsessive‐compulsive disorder (OCD). Despite this, ERP is not widely used by mental health practitioners, and so dissemination of ERP and other empirically supported treatment (ESTs) has become a priority. Even so, utilization of ESTs such as ERP remains below 50% even among therapists who self‐identify as having a cognitive behavioral orientation. Barriers to the acceptance of ERP include practical obstacles such as lack of training and the cost of treatment, but also patient variables such as treatment refusal. It has been estimated that approximately 25% of OCD patients refuse ERP. This paper describes a brief, 4‐session readiness intervention (RI) designed to decrease ERP refusal among patients with OCD. In this study, 12 patients with OCD who had refused ERP were randomized to RI or wait‐list (WL). 86% of participants in the RI condition and 20% of participants in WL condition agreed to begin ERP following the 4‐week period. ERP following RI, but not WL, was associated with a decrease in OCD symptoms comparable to that observed in OCD patients who did not refuse ERP. However, ERP following RI was associated with a high drop‐out rate (50%), a figure that exceeds that typically seen in OCD treatment studies. Techniques to reduce drop‐out as well as directions for future research are discussed.