Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular cloning, nucleotide sequence, and promoter structure of the Acinetobacter calcoaceticus trpFB operon

The trpFB operon from Acinetobacter calcoaceticus encoding the phosphoribosyl anthranilate isomerase and the beta-subunit of tryptophan synthase has been cloned by complementation of a trpB mutation in A. calcoaceticus, identified by deletion analysis, and sequenced. It encodes potential polypeptides of 214 amino acids with a calculated molecular weight of 23,008 (TrpF) and 403 amino acids with a molecular weight of 44,296 (TrpB). The encoded TrpB sequence shows striking homologies to those from other bacteria, ranging from 47% amino acids identity with the Brevibacterium lactofermentum protein and 64% identity with the Caulobacter crescentus protein. The encoded TrpF sequence, on the other hand, is much less homologous to the ones from other species, ranging between 27% identity with the Bacillus subtilis enzyme and 36% identity with the C. crescentus enzyme. The homologies of both polypeptides are evenly distributed over the entire sequences. The codon usage shows the strong preference for A and T in the third positions typical for A. calcoaceticus genes. The trpFB operon appears to be unlinked to trpA. The trpFB promoter has been determined by primer extension analysis of RNA synthesized from the chromosomally and plasmid-encoded trpFB operons. The starting nucleotides are identical in both cases and define the first promoter from A. calcoaceticus. Potential regulatory features are implied by a palindromic element overlapping the -35 consensus box of the promoter.
Learning Retrieval Knowledge from Data

A challenge of future knowledge management and decision support systems is to combine the storage and effective reuse of data, systematically captured as process or system information, with user experience in dealing with problems and non-trivial situations. In CBR, situation-specific user experiences are typically captured in cases. In our approach, cases are linked within a semantic network of more general domain knowledge. In this paper we present a way to automate the construction and dynamical refinement of such a model of case-specific and general knowledge, on the basis of external process data continuously being generated. A data mining method based on a Bayesian Networks approach is used. We are also looking into how the notion of causality, being a central issue in both BNs and model-based AI, can be compared and better understood by relating it to such a combined model.
Cross-track Infrared Sounder (CrIS) satellite observations of tropospheric ammonia

Abstract. Observations of atmospheric ammonia are important in understanding and modelling the impact of ammonia on both human health and the natural environment. We present a detailed description of a robust retrieval algorithm that demonstrates the capabilities of utilizing Cross-track Infrared Sounder (CrIS) satellite observations to globally retrieval ammonia concentrations. Initial ammonia retrieval results using both simulated and real observations show that (i) CrIS is sensitive to ammonia in the boundary layer with peak vertical sensitivity typically around ~ 850–750 hPa (~ 1.5 to 2.5 km), which can dip down close to the surface (~ 900 hPa) under ideal conditions, (ii) it has a minimum detection limit of ~ 1 ppbv (peak profile value typically at the surface), and (iii) the information content can vary significantly with maximum values of ~ 1 degree-of-freedom for signal. Comparisons of the retrieval with simulated "true" profiles show a small positive retrieval bias of 6% with a standard deviation of ~ ± 20% (ranging from ± 12 to ± 30% over the vertical profile). Note that these uncertainty estimates are considered as lower bound values as no potential systematic errors are included in the simulations. The CrIS NH3 retrieval applied over the Central Valley in CA, USA, demonstrates that CrIS correlates well with the spatial variability of the boundary layer ammonia concentrations seen by the nearby Quantum Cascade-Laser (QCL) in situ surface and the Tropospheric Emission Spectrometer (TES) satellite observations as part of the DISCOVER-AQ campaign. The CrIS and TES ammonia observations show quantitatively similar retrieved boundary layer values that are often within the uncertainty of the two observations. Also demonstrated is CrIS's ability to capture the expected spatial distribution in the ammonia concentrations, from elevated values in the Central Valley from anthropogenic agriculture emissions, to much lower values in the unpolluted or clean surrounding mountainous regions. These initial results demonstrate the capabilities of the CrIS satellite to measure ammonia.
On the inverse relationship between ex-ante and ex-post moral hazard: the case of smokers

In the paper I start from the observation that smokers' use of services is less than expected and I suggest that this finding is coherent with an hypothesis: there is a psychological component, leading to increased/reduced consumption if the illness was not/was generated by ex-ante moral hazard.I illustrate the amount and significance of such psychological effect by estimating with Italian survey data, a consumption function for medical consultations and diagnostic screenings, in which smokers represent a group with high ex-ante moral hazard and low ex-post moral hazard, while former smokers have low ex-ante moral hazard and high ex-post moral hazard.The results confirm the theory, though in an asymmetric way: past smokers' consumption shows a strong and significant increase, while current smokers' reduction of use is lower and not always significant.
VDT: A data integration model for web applications

This paper proposes a three-layer model for web applications named VDT (View, Data Processor and Transceiver) and analyzes its performance. It standardizes the design of a web application when fetching data from different sources on both a browser by AJAX (Asynchronous JavaScript and XML) and an agent server. It has the benefits of general hierarchical models such as loosely coupled structure which can fit the varied data sources. Using the VDT model, we implement two web maps applications integrating data obtained from three sources. One gets the data from original sources directly by AJAX API while the other does through an agent server. They are vertical search engines of course. We compare these two applications and conclude that each has its own advantages in the response time, resource consumption and quality of results.
Reducing Audio Noise Using Spectrogram Random Textures

This paper discusses audio enhancement when a strong, additive noise is present only during a known or easily detected period of moderate length (of around one second). The signals may contain intelligible components such as speech or music, and may also contain desired, but unintelligible, background components such as rivers or waterfalls. A first estimate synthesizes the unintelligible components from the noise-free neighboring spectrogram. A second estimate recovers the intelligible components using spectral attenuation. The two estimates are combined using ideas from statistical process control. Tests with audio containing digital camera zoom motor noise, and with simulations, validate the approach
Improved tests for a random effects meta‐regression with a single covariate

The explanation of heterogeneity plays an important role in meta‐analysis. The random effects meta‐regression model allows the inclusion of trial‐specific covariates which may explain a part of the heterogeneity. We examine the commonly used tests on the parameters in the random effects meta‐regression with one covariate and propose some new test statistics based on an improved estimator of the variance of the parameter estimates. The approximation of the distribution of the newly proposed tests is based on some theoretical considerations. Moreover, the newly proposed tests can easily be extended to the case of more than one covariate. In a simulation study, we compare the tests with regard to their actual significance level and we consider the log relative risk as the parameter of interest. Our simulation study reflects the meta‐analysis of the efficacy of a vaccine for the prevention of tuberculosis originally discussed in Berkey et al. The simulation study shows that the newly proposed tests are superior to the commonly used test in holding the nominal significance level. Copyright © 2003 John Wiley & Sons, Ltd.
Female Preschoolers' Verbal and Nonverbal Empathic Responses to Emotional Situations and Facial Expressions

Several conceptualizations about the interdependency of three empathy responses during the empathic arousal process in children were contrasted, i.e., understanding, sharing, and facially expressing another's affect (cognitive, affective, and facial empathy, respectively). Videotaped episodes in which actors portrayed happiness, fear, sadness, and anger were presented to five-year-old girls in three conditions, 16 girls in each condition. In a first condition, emotions were conveyed by the actor's facial expressions, in a second condition by situational events, and in a third condition by both situational events and facial expressions. Girls' affective and cognitive empathy was assessed by asking questions about girls' own feelings and the actor's feelings, respectively. Girls' facial empathy was measured with Ekman and Friesen's Facial Action Coding System. Results corroborated an empathy conceptualization in which affective and facial empathy are mediated by cognitive empathy. An empathy conceptualization in which cognitive and affective empathy are aroused by afferent feedback of involuntary facial empathy was not supported by the results.
If Language Is a Complex Adaptive System, What Is Language Assessment?

Individuals' use of language in contexts emerges from second-to-second processes of activating and integrating traces of past experiences—an interactionist view compatible with the study of language as a complex adaptive system but quite different from the trait-based framework through which measurement specialists investigate validity, establish reliability, and ensure fairness of assessments. This article discusses assessment arguments from an interactionist perspective. We argue that the familiar concepts and methods of assessment that evolved under a trait perspective can be gainfully reconceived in terms of the finer-grained perspective of interactionism, and we illustrate how key ideas relate to familiar practices in language testing.
Performance on the Cognitive Estimation Test in Schizophrenia

The Cognitive Estimation Test (CET) is generally considered to be a measure of executive function, but there is little information with respect to its clinical utility in patients with schizophrenia. In the present investigation, we evaluated the clinical utility of the CET in 42 patients with schizophrenia relative to 42 healthy comparison subjects matched for age, gender, and parental education. Construct validity of the CET was examined though correlation with other tests of executive and nonexecutive cognitive functions. Patients with schizophrenia performed more poorly on the CET compared with the healthy comparison group, which could not be accounted for by greater level of depression in the patient sample. In the schizophrenia group, CET was correlated with measures of executive function but also general intellectual functioning, verbal learning, and auditory attention. CET performance was not associated with depression or overall severity of psychopathology in the patient sample. These findings provide support for the clinical utility of the CET in schizophrenia but indicate that both executive and nonexecutive cognitive functions contribute to performance on the measure. Thus, the integrity of other cognitive processes should be taken into consideration when interpreting the presence of a deficit in cognitive estimation in patients with schizophrenia.
Effects of transition from elementary school to junior high school on early adolescents' self-esteem and perceived competence

Early adolescents' self-esteem and perceived competence in the cognitive, social, and physical domains were examined across the transition from elementary school to junior high school. Self-esteem and perceived social and physical competence were either stable or increased for most children. Cognitive competence was unchanged. It was concluded that, in general, early adolescents' perceptions of self-esteem and competence are not adversely affected by the transition.
Efficacy of MitoTracker Green™ and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues

Chloromethyl‐X‐rosamine (CMXRos) and MitoTracker Green (MTG) have proved to be useful dyes with which to measure mitochondrial function. CMXRos is a lipophilic cationic fluorescent dye that is concentrated inside mitochondria by their negative mitochondrial membrane potential (MMP). MTG fluorescence has been used as a measure of mitochondrial mass independent of MMP. The fluorescence ratio of the two dyes is a relative measure of the MMP independent of mitochondrial mass. Because MTG was recently reported to be sensitive to MMP, we have reevaluated the effects of loss of MMP on MTG and CMXRos fluorescence, using both flow cytometry and laser scanning confocal microscopy (LSCM).
Small Cell Lung Cancer Protooncogene Transcripts in kit Preferential Expression of c-Updated Version

As an initial step to understand rapid growth of small cell lung cancer (SCLC), a complementary DNA library prepared from a SCLC cell line was screened with viral oncogene probes encoding protein-tyrosine ki nases, which are known to play an important role in regulation of cell growth. Fifteen clones hybridizing with \-fms probe were isolated, and, by partial sequence analysis, four of them were identified to be c-kit protooncogenes. Northern blot study demonstrated that most of the SCLC tumors and cell lines expressed c-kit transcripts, while non-SCLC tumors and cell lines did not. Neither amplification nor rearrangement of the ckit gene was demonstrated in SCLC cell lines by Southern blot analysis, however. Our results suggested that c-kit expression in SCLC reflects the unique biological nature of the tumor cells different from non-SCLC and further suggested that the c-kit product may participate in autocrine or paracrine stimulation of SCLC growth.
Six millisecond pulsars detected by the Fermi Large Area Telescope and the radio/gamma-ray connection of millisecond pulsars

We report on the discovery of gamma-ray pulsations from five millisecond pulsars (MSPs) using the Fermi Large Area Telescope (LAT) and timing ephemerides provided by various radio observatories. We also present confirmation of the gamma-ray pulsations from a sixth source, PSR J2051−0827. Five of these six MSPs are in binary systems: PSRs J1713+0747, J1741+1351, J1600−3053 and the two black widow binary pulsars PSRs J0610−2100 and J2051−0827. The only isolated MSP is the nearby PSR J1024−0719, which is also known to emit X-rays. We present X-ray observations in the direction of PSRs J1600−3053 and J2051−0827. While PSR J2051−0827 is firmly detected, we can only give upper limits for the X-ray flux of PSR J1600−3053. There are no dedicated X-ray observations available for the other three objects. The MSPs mentioned above, together with most of the MSPs detected by Fermi, are used to put together a sample of 30 gamma-ray MSPs. This sample is used to study the morphology and phase connection of radio and gamma-ray pulse profiles. We show that MSPs with pulsed gamma-ray emission which is phase-aligned with the radio emission present the steepest radio spectra and the largest magnetic fields at the light cylinder among all MSPs. Also, we observe a trend towards very low, or undetectable, radio linear polarization levels. These properties
Minority identification for imbalanced dataset

Minority identification is an important issue in network security and financial applications. This paper considers the direct maximum reachability distance of an object and the indirect minimum reachability distance of an object for measuring the degree of an object being minority. The data classification is performed by an optimized combination model. We empirically evaluate the proposed approach using a number of UCI data sets, and experiment results demonstrate that our method outperforms the existing methods in terms of the comparisons of ROC curves.
The psychology and economics of attitudes in The Netherlands - 18

Users may download and print one copy of any publication from the public portal for the purpose of private study or research You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal Take down policy If you believe that this document breaches copyright, please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Fairness as Social Responsibility: A Moral Self-Regulation Account of Procedural Justice Enactment

Three studies examined the relationship between moral identity and procedural justice enactment and explored the moderating role of regulatory focus in this relationship. In Study 1, an experimental scenario study, leaders with a strong moral identity were more likely to enact decisions accurately in an employee performance evaluation procedure. This effect emerged in the prevention focus condition, but was absent in the promotion focus condition. In Study 2, an organizational field survey, organizational supervisors' moral identity related to self-reported voice granting, and this effect was pronounced among those with strong (as opposed to weak) dispositional prevention focus. In Study 3, another field study, organizational supervisors' moral identity related to co-worker ratings of voice granting and this effect was again pronounced among supervisors with strong (as opposed to weak) prevention focus. Implications of these findings are discussed in terms of a moral self-regulation account of justice enactment.
The Past in Belgium: Different Memories and Controversial History in a Divided Society?

This contribution focuses on the spirit and the associated behaviours belonging to Belgian society. It deals with the past and, more specifically, with the legacy of the two world wars. In which way did Flemings and Francophones interact? What is the real nature of their relations described from the roots of the many issues and embedded consequences seen even today? Why is there a different vision of the past and how did the situation evolve with the federalization within the country?
The Yeast Nuclear Pore Complex

An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify all components of the yeast NPC (nucleoporins). This involved identifying all the proteins present in a highly enriched NPC fraction, determining which of these proteins were nucleoporins, and localizing each nucleoporin within the NPC. Using these data, we present a map of the molecular architecture of the yeast NPC and provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic transport.
Solvent Free Synthesis , Spectral Studies and Antimicrobial Activities of Substituted ( E )-1-Benzylidene-2-( 4-Methylphenyl ) Hydrazine ’

A series of nine number of substituted (E)-1-benzylidene-2-(4-methylphenyl) hydrazines has been synthesized by condensation of 4-methylphenylhydrazine with substituted benzaldehydes using Fly-ash–H2SO4 catalyst under solvent free condition. This method is experimentally simple, high yielding process with shorter reaction time. The synthesized substituted (E)-1-benzylidene-2-(4-methylphenyl) hydrazines have been assigned by physical constants, UV, IR and NMR spectral data. This UV, IR and NMR spectral data have been utilized for spectral correlation analyses. From the results of spectral correlation analysis assessment of substituent effect have been studied. The antimicrobial activities of all the substituted (E)-1-benzylidene-2-(4-methylphenyl) hydrazines have been studied using disc diffusion method.
Music Tempo Estimation Using Sub-Band Synchrony

Tempo estimation aims at estimating the pace of a musical piece measured in beats per minute. This paper presents a new tempo estimation method that utilizes coherent energy changes across multiple frequency sub-bands to identify the onsets. A new measure, called the sub-band synchrony, is proposed to detect and quantify the coherent amplitude changes across multiple sub-bands. Given a musical piece, our method first detects the onsets using the sub-band synchrony measure. The periodicity of the resulting onset curve, measured using the autocorrelation function, is used to estimate the tempo value. The performance of the sub-band synchrony based tempo estimation method is evaluated on two music databases. Experimental results indicate a reasonable improvement in performance when compared to conventional methods of tempo estimation.
The Experience of Using the Scrum Process in the Production of Learning Objects for Blended Learning

The technological resources used for pedagogical innovation in the form of distance education have increasingly been incorporated into face-to-face education. This article describes the experience of the Federal University of Lavras – Brazil – with new ways to apply technology in face-to-face undergraduate courses. This paper presents (i) the strategy for the selection of course content, which was premised on the diversification of areas of knowledge and on promoting the permanent incorporation of the resources developed in the teaching-learning process, (ii) the organization of the production process of Learning Objects based on the Scrum method, (iii) the set of best practices, inspired by the management of agile software development, as well as the contextual motivation of its use.
Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication

The 42-kD component of the S. cerevisiae spindle pole body (SPB) localizes to the electron-dense central plaque of the SPB. We have cloned the corresponding gene SPC42 (spindle pole component) and show that it is essential. Seven temperature-sensitive (ts) mutants in SPC42 were prepared by error-prone PCR. We found that a change to a proline residue in a potential coiled-coil region of Spc42p was responsible for the ts phenotype in at least three alleles, suggesting that formation of the coiled-coil is essential in normal function. The mutant cells showed a phenotype of predominantly single or bilobed SPBs often with an accumulation of unstructured electron-dense material associated with the bridge structure adjacent to the SPB. This phenotype suggests a defect in SPB duplication. This was confirmed by examining synchronized mutant cells that lose viability when SPB duplication is attempted. Spc42p is a phosphoprotein which shows some cell cycle-regulated phosphorylation. Overexpression of Spc42p causes the formation of a disc- or dome-shaped polymer composed of phosphorylated Spc42p, which is attached to the central plaque and associated with the outer nuclear membrane. Taken together, these data suggest that Spc42p forms a polymeric layer at the periphery of the SPB central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.