Miracle-dz/UniFAR-FTUs huggingface dataset:
Tissue-specific glucose toxicity induces mitochondrial damage in a burn injury model of critical illness

Objective: In critically ill patients, preventing hyperglycemia (HG) with insulin therapy partially prevented organ dysfunction and protected mitochondria. A study in a rabbit model of critical illness indicated that lower blood glucose level, rather than higher insulinemia, is a key factor in such organ protection. In this model, we now investigated the impact of blood glucose lowering vs. hyperinsulinemia (HI) on mitochondria in relation to organ damage. We assessed whether such effects on mitochondria are mediated indirectly via organ perfusion or directly via reducing cellular glucose toxicity. Design: Prospective, randomized laboratory investigation. Setting: University laboratory. Subjects: Three-month-old male rabbits. Interventions: After induction of critical illness by burn injury, followed by fluid-resuscitation and parenteral nutrition, rabbits were allocated to four groups, each a combination of normal or elevated blood glucose levels with normal or elevated insulin levels. This required alloxan administration, immediately followed by intravenous insulin and glucose infusions titrated to the respective targets. Measurements and Main Results: In liver, the reduced damage by glucose lowering was not explained by better perfusion/oxygen delivery. Abnormal mitochondrial ultrastructure and function was present in the two hyperglycemic groups, most pronounced with concomitant HI. Affected mitochondrial respiratory chain enzyme activities were reduced to 25% to 62% of values in healthy rabbits, in the presence of up to five-fold increased tissue levels of glucose. This was accompanied by elevated levels of dicarbonyls, which may mediate direct toxicity of cellular glucose overload and accelerated glycolysis. The abnormalities were also present in myocardium, although to a lesser extent, and absent in skeletal muscle. Conclusions: In a rabbit model of critical illness, HG evokes cellular glucose overload in liver and myocardium inducing mitochondrial dysfunction, which explained the HG-induced organ damage. Maintenance of normoglycemia, but not HI, protects against such mitochondrial and organ damage.
Spanish and Chilean Standardizations of the Personality Assessment Inventory: the Influence of Sex

Abstract There is growing interest in the adaptation of psychological questionnaires in different countries, due to the need for cross-cultural research using the same tests adapted to diverse populations. This paper presents the standardization of the Personality Assessment Inventory (PAI; Morey, 1991, 2007) in Spain and Chile (both Spanish-speaking countries). The Spanish sample was made up of 940 people (461 men and 479 women), and the Chilean sample of 569 people (231 men and 338 women). Results revealed that the Chilean means were higher than those of the Spanish sample at confidence level 99.9%, although the associated effect sizes were generally small to moderate (partial eta-square between 0.008 and 0.187). Sex differences in the variables evaluated were commented on, and the importance of cross-cultural research and the influence of sex on personality and psychopathology variables were discussed.
Increasing the oral bioavailability of poorly water-soluble carbamazepine using immediate-release pellets supported on SBA-15 mesoporous silica

Background and methods: The aim of this study was to develop an immediate-release pellet formulation with improved drug dissolution and adsorption. Carbamazepine, a poorly water-soluble drug, was adsorbed into mesoporous silica (SBA-15-CBZ) via a wetness impregnation method and then processed by extrusion/spheronization into pellets. Physicochemical characterization of the preparation was carried out by scanning electron microscopy, transmission electron microscopy, nitrogen adsorption, small-angle and wide-angle x-ray diffraction, and differential scanning calorimetry. Flowability and wettability of the drug-loaded silica powder were evaluated by bulk and tapped density and by the angle of repose and contact angle, respectively. The drug-loaded silica powder was formulated into pellets to improve flowability. Results: With maximum drug loading in SBA-15 matrices determined to be 20% wt, in vitro release studies demonstrated that the carbamazepine dissolution rate was notably improved from both the SBA-15 powder and the corresponding pellets as compared with the bulk drug. Correspondingly, the oral bioavailability of SBA-15-CBZ pellets was increased considerably by 1.57-fold in dogs (P < 0.05) compared with fast-release commercial carbamazepine tablets. Conclusion: Immediate-release carbamazepine pellets prepared from drug-loaded silica provide a feasible approach for development of a rapidly acting oral formulation for this poorly water-soluble drug and with better absorption.
Effects of Shear Stress on the Number and Function of Endothelial Progenitor Cells Adhered to Specific Matrices

Purpose The aim of this study was to screen specific adherent matrix for endothelial progenitor cells (EPCs), which can be used for antibody capturing stents. Methods In this study, the adhesion of EPCs on different matrices containing three different antibodies, VEGFR-2, CD34, CD133, was observed under shear stress in a flow chamber. Nitric oxide (NO) release, cell proliferation and the retention rate of EPCs, were measured separately. Results The results demonstrated that shear stress within a certain range can promote proliferation and NO secretion of EPCs. Under the same shear stress, the EPCs showed stronger adhesion on matrix-containing CD133 antibody than on the other matrices. Conclusions CD133 antibody has the potential application for EPCs capture.
Imaging arteriovenous fistulas.

OBJECTIVE Arteriovenous fistulas (AVFs) are abnormal communications with shunting of blood from an artery to a vein. AVFs mainly involve the peripheral vascular system but can affect virtually any organ or system in the body. They may be congenital, created surgically for hemodialysis, or caused by pathologic processes. This review describes the diverse origins of AVFs and illustrates the spectrum of imaging findings at radiography, ultrasound, CT, MRI, and digital angiography for AVFs in different locations: the CNS, thorax, abdomen, musculoskeletal system, and peripheral vascular system. We also discuss syndromes associated with AVFs and describe recent interventional techniques for treating AVFs. CONCLUSION Familiarity with the spectrum of imaging findings in AVFs is essential for the accurate interpretation of images and facilitates diagnosis and therapeutic management. Radiologists can play a critical role in the diagnosis and treatment of AVFs. Digital angiography is helpful in elaborating a vascular map for endovascular treatment.
Efficient reading.

Feel lonely? What about reading books? Book is one of the greatest friends to accompany while in your lonely time. When you have no friends and activities somewhere and sometimes, reading book can be a great choice. This is not only for spending the time, it will increase the knowledge. Of course the b=benefits to take will relate to what kind of book that you are reading. And now, we will concern you to try reading efficient reading as one of the reading material to finish quickly.
Techniques for automatically correcting words in text

Research aimed at correcting words in text has focused on three progressively more difficult problems:(1) nonword error detection; (2) isolated-word error correction; and (3) context-dependent work correction. In response to the first problem, efficient pattern-matching and n-gram analysis techniques have been developed for detecting strings that do not appear in a given word list. In response to the second problem, a variety of general and application-specific spelling correction techniques have been developed. Some of them were based on detailed studies of spelling error patterns. In response to the third problem, a few experiments using natural-language-processing tools or statistical-language models have been carried out. This article surveys documented findings on spelling error patterns, provides descriptions of various nonword detection and isolated-word error correction techniques, reviews the state of the art of context-dependent word correction techniques, and discusses research issues related to all three areas of automatic error correction in text.
Cerebrospinal fluid B‐cell expansion in longitudinally extensive transverse myelitis associated with neuromyelitis optica immunoglobulin G

A first episode of central nervous system (CNS) demyelination may represent heterogeneous entities such as acute disseminated encephalomyelitis, clinically isolated syndrome, neuromyelitis optica (NMO), or multiple sclerosis. As new immune therapies become available, it is increasingly important to make an early diagnosis. Autoantibodies such as NMO immunoglobulin G (IgG) and myelin oligodendrocyte glycoprotein IgG are increasingly being employed to define subgroups of CNS demyelination or guide treatment. Similarly, cerebrospinal fluid (CSF) immunophenotyping can demonstrate B‐lymphocyte subpopulation expansion, which has been used to guide therapy in other autoimmune CNS disorders. We present a report on a 15‐year‐old male with longitudinally extensive transverse myelitis with magnetic resonance imaging findings of oedema, cavitation, and gadolinium enhancement. NMO‐IgG and aquaporin 4 IgG were positive; thus, we diagnosed a limited form of NMO. Acute CSF immunophenotyping revealed a 3.6% expansion of CD19 B‐cell populations, whereas a comparison group of five children (4 males, age range 2–15y; mean age 7y) with other neurological disorders showed only a 0.51% expansion (SD 0.25%). In view of the diagnosis of a ‘limited form of neuromyelitis optica’, we therefore elected to treat him aggressively from the outset with a prolonged steroid regimen and mycophenylate mofetil. This case demonstrates a correlation between autoantibody production and CSF B lymphocyte expansion in an individual with CNS demyelination. These approaches could be used in individuals with a first episode of CNS demyelination to help delineate immunological subgroups and guide treatment.
Resistance analysis of genotype 3 hepatitis C virus indicates subtypes inherently resistant to nonstructural protein 5A inhibitors

Hepatitis C virus (HCV) genotype (gt) 3 is highly prevalent globally, with non‐gt3a subtypes common in Southeast Asia. Resistance‐associated substitutions (RASs) have been shown to play a role in treatment failure. However, the role of RASs in gt3 is not well understood. We report the prevalence of RASs in a cohort of direct‐acting antiviral treatment‐naive, gt3‐infected patients, including those with rarer subtypes, and evaluate the effect of these RASs on direct‐acting antivirals in vitro. Baseline samples from 496 gt3 patients enrolled in the BOSON clinical trial were analyzed by next‐generation sequencing after probe‐based enrichment for HCV. Whole viral genomes were analyzed for the presence of RASs to approved direct‐acting antivirals. The resistance phenotype of RASs in combination with daclatasvir, velpatasvir, pibrentasvir, elbasvir, and sofosbuvir was measured using the S52 ΔN gt3a replicon model. The nonstructural protein 5A A30K and Y93H substitutions were the most common at 8.9% (n = 44) and 12.3% (n = 61), respectively, and showed a 10‐fold and 11‐fold increase in 50% effect concentration for daclatasvir compared to the unmodified replicon. Paired RASs (A30K + L31M and A30K + Y93H) were identified in 18 patients (9 of each pair); these combinations were shown to be highly resistant to daclatasvir, velpatasvir, elbasvir, and pibrentasvir. The A30K + L31M combination was found in all gt3b and gt3g samples. Conclusion: Our study reveals high frequencies of RASs to nonstructural protein 5A inhibitors in gt3 HCV; the paired A30K + L31M substitutions occur in all patients with gt3b and gt3g virus, and in vitro analysis suggests that these subtypes may be inherently resistant to all approved nonstructural protein 5A inhibitors for gt3 HCV. (Hepatology 2018).
The development of complex tooth shape in reptiles

Reptiles have a diverse array of tooth shapes, from simple unicuspid to complex multicuspid teeth, reflecting functional adaptation to a variety of diets and eating styles. In addition to cusps, often complex longitudinal labial and lingual enamel crests are widespread and contribute to the final shape of reptile teeth. The simplest shaped unicuspid teeth have been found in piscivorous or carnivorous ancestors of recent diapsid reptiles and they are also present in some extant carnivores such as crocodiles and snakes. However, the ancestral tooth shape for squamate reptiles is thought to be bicuspid, indicating an insectivorous diet. The development of bicuspid teeth in lizards has recently been published, indicating that the mechanisms used to create cusps and crests are very distinct from those that shape cusps in mammals. Here, we introduce the large variety of tooth shapes found in lizards and compare the morphology and development of bicuspid, tricuspid, and pentacuspid teeth, with the aim of understanding how such tooth shapes are generated. Next, we discuss whether the processes used to form such morphologies are conserved between divergent lizards and whether the underlying mechanisms share similarities with those of mammals. In particular, we will focus on the complex teeth of the chameleon, gecko, varanus, and anole lizards using SEM and histology to compare the tooth crown morphology and embryonic development.
Black Phosphorus Nanosheets as a Neuroprotective Nanomedicine for Neurodegenerative Disorder Therapy

Transition‐metal dyshomeostasis is recognized as a critical pathogenic factor at the onset and progression of neurodegenerative disorder (ND). Excess transition‐metal ions such as Cu2+ can catalyze the generation of cytotoxic reactive oxygen species and thereafter induce neuronal cell apoptosis. Exploring new chelating agents, which are not only capable of capturing excess redox‐active metal, but can also cross the blood–brain barrier (BBB), are highly desired for ND therapy. Herein, it is demonstrated that 2D black phosphorus (BP) nanosheets can capture Cu2+ efficiently and selectively to protect neuronal cells from Cu2+‐induced neurotoxicity. Moreover, both in vitro and in vivo studies show that the BBB permeability of BP nanosheets is significantly improved under near‐infrared laser irradiation due to their strong photothermal effect, which overcomes the drawback of conventional chelating agents. Furthermore, the excellent biocompatibility and stability guarantee the biosafety of BP in future clinical applications. Therefore, these features make BP nanosheets have the great potential to work as an efficient neuroprotective nanodrug for ND therapy.
Alcohol and risk of Parkinson's disease in a large, prospective cohort of men and women

Addictive behaviors, such as cigarette smoking and coffee drinking, have been associated with a reduced risk of Parkinson's disease (PD). Whether alcohol consumption is also associated with PD risk is less certain. We prospectively followed 132,403 participants in the Cancer Prevention Study II Nutrition Cohort from 1992 to 2005. Alcohol intake was assessed at baseline. Incident cases of PD (n = 605; 389 male and 216 female) were confirmed by treating physicians and medical record review. Relative risks (RRs) were estimated using proportional hazards models, adjusting for age, smoking, and other risk factors. Alcohol consumption was not significantly associated with PD risk. After adjustment for age, smoking, and other risk factors, the RR comparing men consuming 30 or more grams of alcohol per day (highest category) to nondrinker men was 1.29 (95% confidence interval [CI]: 0.90, 1.86; P trend: 0.40), and the RR comparing women consuming 15 or more grams of alcohol (highest category) per day to nondrinker women was 0.77 (95% CI: 0.41, 1.45; P trend: 0.87). Consumption of beer, wine, or liquor was also not associated with PD risk. The results of this large, prospective study do not support an association between alcohol intake and risk of PD. © 2012 Movement Disorder Society
E* Interpolated Graph Replanner

Smoothness: propagate a continuous-domain wavefront through the environment, modulate its speed according to traversability ⇒ monotonically increasing crossing-time map ⇒ smooth navigation function Local Repairs: region of influence of each location ⇒ recompute only the shadow of modified nodes Related Work:Field-D* also interpolates, but E* interpolation is user-configurable and it performs full tracking of the upwind dependency structure. Open Source:C++ implementation available on Sourceforge, with test programs and examples of integration into robotic systems
Subjective and Objective Risk Tolerance: Implications for Optimal Portfolios

The distinction between subjective and objective risk tolerance is illustrated by expected utility analyses of portfolios. Optimal portfolios were derived for one, 5, and 20 year investment horizons for 6 major financial asset categories. The important aspects of objective risk tolerance are the proportion of an investor's total wealth (including human wealth) in financial assets, and the investment horizon. Even investors with very low subjective risk tolerance levels should have aggressive portfolios if their horizons are 20 years or more.
Online computation and competitive analysis

Preface 1. Introduction to competitive analysis: the list accessing problem 2. Introduction to randomized algorithms: the list accessing problem 3. Paging: deterministic algorithms 4. Paging: randomized algorithms 5. Alternative models for paging: beyond pure competitive analysis 6. Game theoretic foundations 7. Request - answer games 8. Competitive analysis and zero-sum games 9. Metrical task systems 10. The k-server problem 11. Randomized k-server algorithms 12. Load-balancing 13. Call admission and circuit-routing 14. Search, trading and portfolio selection 15. Competitive analysis and decision making under uncertainty Appendices Bibliography Index.
Thrombospondin-induced tumor cell migration: haptotaxis and chemotaxis are mediated by different molecular domains

Thrombospondin induces the migration of human melanoma and carcinoma cells. Using a modified Boyden chamber assay, tumor cells migrated to a gradient of soluble thrombospondin (chemotaxis). Checkerboard analysis indicated that directional migration was induced 27-fold greater than stimulation of random motility. Tumor cells also migrated in a dose- dependent manner to a gradient of substratum-bound thrombospondin (haptotaxis). A series of human melanoma and carcinoma cells were compared for their relative motility stimulation by thrombospondin haptotaxis vs. chemotaxis. Some cell lines exhibited a stronger haptotactic response compared to their chemotactic response while other lines exhibited little or no migration response to thrombospondin. Human A2058 melanoma cells which exhibit a strong haptotactic and chemotactic response to thrombospondin were used to study the structural domains of thrombospondin required for the response. Monoclonal antibody C6.7, which binds to the COOH-terminal region of thrombospondin, inhibited haptotaxis in a dose-dependent optimal manner. C6.7 had no significant effect on thrombospondin chemotaxis. In contrast, monoclonal antibody A2.5, heparin, and fucoidan, which bind to the NH2-terminal heparin-binding domain of thrombospondin, inhibited thrombospondin chemotaxis but not haptotaxis. Monoclonal antibody A6.1 directed against the internal core region of thrombospondin had no significant effect on haptotaxis or chemotaxis. Synthetic peptides GRGDS (50 micrograms/ml), but not GRGES, blocked tumor cell haptotaxis on fibronectin, but had minimal effect on thrombospondin or laminin haptotaxis. The 140-kD fragment of thrombospondin lacking the heparin- binding amino-terminal region retained the property to fully mediate haptotaxis but not chemotaxis. When the COOH region of the 140-kD fragment, containing the C6.7-binding site, was cleaved off, the resulting 120-kD fragment (which retains the RGDA sequence) failed to induce haptotaxis. Separate structural domains of thrombospondin are therefore required for tumor cell haptotaxis vs. chemotaxis. This may have implications during hematogenous cancer metastases formation.
Hepatic inositol 1,4,5 trisphosphate receptor type 1 mediates fatty liver

Fatty liver is the most common type of liver disease, affecting nearly one third of the U.S. population and more than half a billion people worldwide. Abnormalities in endoplasmic reticulum (ER) calcium handling and mitochondrial function each have been implicated in abnormal lipid droplet formation. Here we show that the type 1 isoform of the inositol 1,4,5‐trisphosphate receptor (InsP3R1) specifically links ER calcium release to mitochondrial calcium signaling and lipid droplet formation in hepatocytes. Moreover, liver‐specific InsP3R1 knockout mice have impaired mitochondrial calcium signaling, decreased hepatic triglycerides, and reduced lipid droplet formation and are resistant to the development of fatty liver. Patients with nonalcoholic steatohepatitis, the most malignant form of fatty liver, have increased hepatic expression of InsP3R1, and the extent of ER–mitochondrial colocalization correlates with the degree of steatosis in human liver biopsies. Conclusion: InsP3R1 plays a central role in lipid droplet formation in hepatocytes, and the data suggest that it is involved in the development of human fatty liver disease. (Hepatology Communications 2017;1:23–35)
Engineering Flat Gain Tunable Raman-Parametric Hybrid L-Band Amplifier for Narrow Band Multi-Channel Terabits System

Abstract We present a model of a Raman-parametric hybrid amplifier for flat gain amplification of narrowband Dense Wavelength Division Multiplexed (DWDM) terabits capacity system. In the proposed configuration Raman pump has been cascaded with one – pump parametric amplifier. While Raman amplifier typically uses a long length single mode fiber as a gain medium, the Fiber Optical Parametric Amplifiers (FOPA) uses a small length of Highly Non-Linear Fibers (HNLF). This allows for maximum optimization of the gain achieved using Raman as well as parametric processes. We focus on the signal degradation due to non-linear crosstalk arising because of multi-pump configuration, in particular, due to the generated idlers within the operational bandwidth. As the channel spacing is reduced, fiber non-linearities increase significantly. The dilemma to use the fewer number of high powered pumps Vs, the use of increased number of relatively low powered pumps has been investigated for DWDM system. The optimization of the pump powers has been evaluated in terms of signal degradation, gain, gain variation and OSNR. It has been demonstrated that a maximum gain of 45.38 dB for 96×40 Gbps, 25 GHz system is attainable up to 200 km of transmission distance while maintaining OSNR>18 dB using the proposed hybrid. No gain compensation or gain optimization technique has been used. The findings of this work establish flat gain in narrowband DWDM systems is achievable through proper optimization of pump wavelengths and their powers.