Miracle-dz/UniFAR-FTUs huggingface dataset:
Neurotropin® Accelerates the Differentiation of Schwann Cells and Remyelination in a Rat Lysophosphatidylcholine-Induced Demyelination Model

Neurotropin® (NTP), a non-protein extract of inflamed rabbit skin inoculated with vaccinia virus, is clinically used for the treatment of neuropathic pain in Japan and China, although its effect on peripheral nerve regeneration remains to be elucidated. The purpose of this study was to investigate the effects of NTP on Schwann cells (SCs) in vitro and in vivo, which play an important role in peripheral nerve regeneration. In SCs, NTP upregulated protein kinase B (AKT) activity and Krox20 and downregulated extracellular signal-regulated kinase1/2 activity under both growth and differentiation conditions, enhanced the expression of myelin basic protein and protein zero under the differentiation condition. In a co-culture of dorsal root ganglion neurons and SCs, NTP accelerated myelination of SCs. To further investigate the influence of NTP on SCs in vivo, lysophosphatidylcholine was injected into the rat sciatic nerve, leading to the focal demyelination. After demyelination, NTP was administered systemically with an osmotic pump for one week. NTP improved the ratio of myelinated axons and motor, sensory, and electrophysiological function. These findings reveal novel effects of NTP on SCs differentiation in vitro and in vivo, and indicate NTP as a promising treatment option for peripheral nerve injuries and demyelinating diseases.
Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells

Significance Breast cancers often contain regions of reduced O2 availability, leading to increased activity of hypoxia-inducible factors (HIFs). Here, we demonstrate that HIFs activate transcription of the Rho family member RHOA and Rho kinase 1 (ROCK1) genes, leading to cytoskeletal changes that underlie the invasive cancer cell phenotype. ROCK1 is a kinase that regulates myosin light-chain activity, leading to actin-myosin contraction, which is the basis for cell movement. Coordinately increased levels of RhoA and ROCK1 mRNA in human breast cancers predicted patient mortality. These results demonstrate that a microenvironmental stimulus, hypoxia, can activate a critical signal transduction pathway, independent of genomic alterations, to drive cancer progression. Overexpression of Rho kinase 1 (ROCK1) and the G protein RhoA is implicated in breast cancer progression, but oncogenic mutations are rare, and the molecular mechanisms that underlie increased ROCK1 and RhoA expression have not been determined. RhoA-bound ROCK1 phosphorylates myosin light chain (MLC), which is required for actin-myosin contractility. RhoA also activates focal adhesion kinase (FAK) signaling. Together, these pathways are critical determinants of the motile and invasive phenotype of cancer cells. We report that hypoxia-inducible factors coordinately activate RhoA and ROCK1 expression and signaling in breast cancer cells, leading to cell and matrix contraction, focal adhesion formation, and motility through phosphorylation of MLC and FAK. Thus, intratumoral hypoxia acts as an oncogenic stimulus by triggering hypoxia-inducible factor → RhoA → ROCK1 → MLC → FAK signaling in breast cancer cells.
Genome-Wide Characterization of Heat-Shock Protein 70s from Chenopodium quinoa and Expression Analyses of Cqhsp70s in Response to Drought Stress

Heat-shock proteins (HSPs) are ubiquitous proteins with important roles in response to biotic and abiotic stress. The 70-kDa heat-shock genes (Hsp70s) encode a group of conserved chaperone proteins that play central roles in cellular networks of molecular chaperones and folding catalysts across all the studied organisms including bacteria, plants and animals. Several Hsp70s involved in drought tolerance have been well characterized in various plants, whereas no research on Chenopodium quinoa HSPs has been completed. Here, we analyzed the genome of C. quinoa and identified sixteen Hsp70 members in quinoa genome. Phylogenetic analysis revealed the independent origination of those Hsp70 members, with eight paralogous pairs comprising the Hsp70 family in quinoa. While the gene structure and motif analysis showed high conservation of those paralogous pairs, the synteny analysis of those paralogous pairs provided evidence for expansion coming from the polyploidy event. With several subcellular localization signals detected in CqHSP70 protein paralogous pairs, some of the paralogous proteins lost the localization information, indicating the diversity of both subcellular localizations and potential functionalities of those HSP70s. Further gene expression analyses revealed by quantitative polymerase chain reaction (qPCR) analysis illustrated the significant variations of Cqhsp70s in response to drought stress. In conclusion, the sixteen Cqhsp70s undergo lineage-specific expansions and might play important and varied roles in response to drought stress.
Regulation of the beta-1,3-glucanase system in Penicillium italicum: glucose repression of the various enzymes

The microscopic fungus Penicillium italicum when grown in a synthetic liquid medium produced at least three enzymes with beta-1,3-glucanase activity which were separated by diethylaminoethyl-Sephadex column chromatography. These were named beta-1,3-glucanases I, II, and III respective to their order of elution from the column. A tentative characterization of these three enzymes indicated that they have different modes of action; the first one is an endoglucanase, the second is an exoglucanase, and the third probably has both mechanisms of action. Glucose had a repressive effect on all three enzymes. Only small amounts of beta-1,3-glucanases II and III were present in the cells when they were actively growing in the presence of this sugar. However, when the cells were transferred to a medium low in glucose, a significant increase in the specific activity of beta-1,3-glucanase took place; this was due in part to a much more active production of beta-1,3-glucanases II and III and in part to the appearance of beta-1,3-glucanase I, which could only be detected after more than 12 h of incubation in this medium. The results are discussed in the context of possible beta-1,3-glucanase functions in the fungal cells.
The Influence of Stressor Exposure and Psychosocial Resources on the Age–Anger Relationship

Objectives: This study examined the processes linking age, stressor exposure, psychosocial coping resources, and two dimensions of anger proneness (i.e., experienced anger and expressed anger). Method: Longitudinal change regression analysis of data from a two-wave community panel study including a sample of people aged 18 to 93 (N = 1,473) is performed. Results: Age is significantly associated with declines in both experienced anger and expressed anger over the 3-year study period. These associations are substantially mediated by the lower levels of chronic stressors and discrimination-related stressors experienced among older adults. In contrast, self-esteem amplifies the association between age and expressed anger. Discussion: These findings clarify the circumstances in which age matters most for changes over time in the experience and expression of anger. They highlight how certain forms of stressor exposure and psychosocial resources are linked with anger proneness and in ways that vary by age.
Sensory-Aware Multimodal Fusion for Word Semantic Similarity Estimation

Traditional semantic models are disembodied from the human perception and action. In this work, we attempt to address this problem by grounding semantic representations of words to the acoustic and visual modalities. Specifically we estimate multimodal word representations via the fusion of auditory and visual modalities with the text modality. We employ middle and late fusion of representations with modality weights assigned to each of the unimodal representations. We also propose a fusion method that assigns different weights to each word, based on how relevant that word is for the audio and visual modalities. The proposed methods are evaluated for the task of semantic similarity computation between words. To our knowledge, this is the first work that combines text, audio and visual features for the computation of multimodal semantic word representations. Multimodal models outperform the unimodal models, indicating the importance of multimodal fusion and perceptual grounding.
Cyanine 5.5 Conjugated Nanobubbles as a Tumor Selective Contrast Agent for Dual Ultrasound-Fluorescence Imaging in a Mouse Model

Nanobubbles and microbubbles are non-invasive ultrasound imaging contrast agents that may potentially enhance diagnosis of tumors. However, to date, both nanobubbles and microbubbles display poor in vivo tumor-selectivity over non-targeted organs such as liver. We report here cyanine 5.5 conjugated nanobubbles (cy5.5-nanobubbles) of a biocompatible chitosan–vitamin C lipid system as a dual ultrasound-fluorescence contrast agent that achieved tumor-selective imaging in a mouse tumor model. Cy5.5-nanobubble suspension contained single bubble spheres and clusters of bubble spheres with the size ranging between 400–800 nm. In the in vivo mouse study, enhancement of ultrasound signals at tumor site was found to persist over 2 h while tumor-selective fluorescence emission was persistently observed over 24 h with intravenous injection of cy5.5-nanobubbles. In vitro cell study indicated that cy5.5-flurescence dye was able to accumulate in cancer cells due to the unique conjugated nanobubble structure. Further in vivo fluorescence study suggested that cy5.5-nanobubbles were mainly located at tumor site and in the bladder of mice. Subsequent analysis confirmed that accumulation of high fluorescence was present at the intact subcutaneous tumor site and in isolated tumor tissue but not in liver tissue post intravenous injection of cy5.5-nanobubbles. All these results led to the conclusion that cy5.5-nanobubbles with unique crosslinked chitosan–vitamin C lipid system have achieved tumor-selective imaging in vivo.
HeteroMF: recommendation in heterogeneous information networks using context dependent factor models

With the growing amount of information available online, recommender systems are starting to provide a viable alternative and complement to search engines, in helping users to find objects of interest. Methods based on Matrix Factorization (MF) models are the state-of-the-art in recommender systems. The input to MF is user feedback, in the form of a rating matrix. However, users can be engaged in interactions with multiple types of entities across different contexts, leading to multiple rating matrices. In other words, users can have interactions in a heterogeneous information network. Generally, in a heterogeneous network, entities from any two entity types can have interactions with a weight (rating) indicating the level of endorsement. Collective Matrix Factorization (CMF) has been proposed to address the recommendation problem in heterogeneous networks. However, a main issue with CMF is that entities share the same latent factor across different contexts. This is particularly problematic in two cases: Latent factors for entities that are cold-start in a context will be learnt mainly based on the data from other contexts where these entities are not cold-start, and therefore the factors are not properly learned for the cold-start context. Also, if a context has more data compared to another context, then the dominant context will dominate the learning process for the latent factors for entities shared in these two contexts. In this paper, we propose a context-dependent matrix factorization model, HeteroMF, that considers a general latent factor for entities of every entity type and context-dependent latent factors for every context in which the entities are involved. We learn a general latent factor for every entity and transfer matrices for every context to convert the general latent factors into a context-dependent latent factor. Experiments on two real life datasets from Epinions and Flixster demonstrate that HeteroMF substantially outperforms CMF, particularly for cold-start entities and for contexts where interactions in one contexts are dominated by other contexts.
Dual hop wireless communications over Nakagami fading

We study the performance of dual-hop wireless communications systems with non-regenerative relays over Nakagami-m fading channels. Deriving tight upper bounds for the end-to-end SNR, important performance criteria such as average end-to-end SNR and error probability are studied. Numerical and computer simulations results are presented to verify the accuracy of the proposed mathematical analysis and to compare our results with previously published ones.
Registration of 3D facial surfaces using covariance matrix pyramids

Registration of 3D facial surfaces means establishing point-to-point correspondence between two 3D facial surfaces. Difficulties typical for the registration of 3D facial surfaces are varying illumination, pose or viewpoint changes, varying facial expressions, and different appearance of individuals. In this work we propose to use a covariance matrix as descriptor for the neighborhood of a salient point in a face. It encodes the variance of the channels, such as red, green, blue, depth, etc., their correlations with each other, and spatial layout, while filtering out the influence of the disturbing effects mentioned above. A pyramidal approach is applied where first the location of a corresponding point is computed roughly and then the position is gradually refined. The method does not require any training. Particle Swarm Optimization makes the search for corresponding points more efficient. Results with a challenging dataset confirm that the approach works greatly for a variety of disturbing effects.
SAME: A students' daily activity mobility model for campus delay-tolerant networks

Mobility model plays a crucial part in wireless network simulation or mathematical analysis, especially for human mobility because real mobility network constructed by human is hard to trace, measure or apply to different scenarios. For delay-tolerant networks, mobility model also affects the frequency and duration of opportunities for data transfer between nodes, which is a key factor of network performance, so whether we can choose a model according to realistic scenes is important for the accuracy and practicability of simulation. In this paper, we propose a new mobility model of students' daily activities based on the analysis and conclusion of students' habits and customs in campus environments, which is able to produce inter-contact time, contact time distribution and other contact information. We validate the movement model by using the ONE simulator comparing with other movement models and tracing data from real-world measurement experiments. Results show that SAME is consistent with actual student behavior characteristics.
Effects of the oral Janus kinase inhibitor tofacitinib on patient-reported outcomes in patients with active rheumatoid arthritis: results of two Phase 2 randomised controlled trials.

OBJECTIVES Tofacitinib is an oral Janus kinase inhibitor for the treatment of rheumatoid arthritis (RA). Here we investigated the effects of tofacitinib on patient-reported outcomes (PRO) in patients with active RA. METHODS Two, 6-month, double-blind, placebo-controlled Phase 2b studies were performed. The combination study evaluated patients with inadequate response to methotrexate who received tofacitinib 1-15 mg twice daily (BID), 20 mg once daily or placebo, on background methotrexate. In the monotherapy study, patients with inadequate response to disease-modifying anti-rheumatic drugs received tofacitinib 1-15 mg BID, adalimumab 40 mg once every other week or placebo. PROs measured were: Patient's Assessment of Arthritis Pain (PAAP), Patient's Assessment of Disease Activity, HAQ-DI, FACIT-F and SF-36. RESULTS In the combination study (n=507), significant improvements (p<0.05) versus placebo were observed at Week 12 in PAAP (visual analogue scale) and HAQ-DI for all tofacitinib groups. In the monotherapy study (n=384), significant improvements in PAAP were observed at Week 12 for tofacitinib 5, 10 and 15 mg BID, and in HAQ-DI for tofacitinib 3, 5, 10 and 15 mg BID. Significant improvements versus placebo were seen at Week 2 in PAAP (both studies) and HAQ‑DI (monotherapy study) with tofacitinib, and were maintained throughout each study. In both studies, improvements in several domains of the SF-36 in the tofacitinib groups were observed at Weeks 12 and 24. CONCLUSIONS In patients with active RA, tofacitinib, either in combination with methotrexate or as monotherapy, demonstrated rapid and sustained improvement in pain, physical functioning and health-related quality of life.
Durability and compressive strength of blast furnace slag-based cement grout for special geotechnical applications

Special foundations, most prominently micropiles and soil anchors, are frequently used in construction today. In Spain, the grout for these special technical applications is generally prepared with portland cement, although the codes and standards in place stipulate only the minimum compressive strength required, with no mention of cement type. Those texts also establish a range of acceptable water:cement ratios. In the present study, durability and compressive strength in cement grout prepared with blast furnace slag cement at different w/c ratios are characterised and compared to the findings for a reference portland cement grout. The results show that slag grout exhibits greater durability than the portland cement material and complies with the compressive strength requirements laid down in the respective codes.
Cardio-Metabolic Effects of HIV Protease Inhibitors (Lopinavir/Ritonavir)

Although antiretroviral treatment decreases HIV-AIDS morbidity/mortality, long-term side effects may include the onset of insulin resistance and cardiovascular diseases. However, the underlying molecular mechanisms responsible for highly active antiretroviral therapy (HAART)-induced cardio-metabolic effects are poorly understood. In light of this, we hypothesized that HIV protease inhibitor (PI) treatment (Lopinavir/Ritonavir) elevates myocardial oxidative stress and concomitantly inhibits the ubiquitin proteasome system (UPS), thereby attenuating cardiac function. Lopinavir/Ritonavir was dissolved in 1% ethanol (vehicle) and injected into mini-osmotic pumps that were surgically implanted into Wistar rats for 8 weeks vs. vehicle and sham controls. We subsequently evaluated metabolic parameters, gene/protein markers and heart function (ex vivo Langendorff perfusions). PI-treated rats exhibited increased serum LDL-cholesterol, higher tissue triglycerides (heart, liver), but no evidence of insulin resistance. In parallel, there was upregulation of hepatic gene expression, i.e. acetyl-CoA carboxylase β and 3-hydroxy-3-methylglutaryl-CoA-reductase, key regulators of fatty acid oxidation and cholesterol synthesis, respectively. PI-treated hearts displayed impaired cardiac contractile function together with attenuated UPS activity. However, there was no significant remodeling of hearts exposed to PIs, i.e. lack of ultrastructural changes, fibrosis, cardiac hypertrophic response, and oxidative stress. Western blot analysis of PI-treated hearts revealed that perturbed calcium handling may contribute to the PI-mediated contractile dysfunction. Here chronic PI administration led to elevated myocardial calcineurin, nuclear factor of activated T-cells 3 (NFAT3), connexin 43, and phosphorylated phospholamban, together with decreased calmodulin expression levels. This study demonstrates that early changes triggered by PI treatment include increased serum LDL-cholesterol levels together with attenuated cardiac function. Furthermore, PI exposure inhibits the myocardial UPS and leads to elevated calcineurin and connexin 43 expression that may be associated with the future onset of cardiac contractile dysfunction.
Diet richness of invasive Indo-Pacific lionfish revealed by DNA barcoding

Indo-Pacific lionfish Pterois spp. have recently invaded marine habitats throughout the western Atlantic, Gulf of Mexico, and Caribbean Sea. Their unusual hunting behaviour suggests that they could prey on most fish species within their gape size limits. However, few prey species have been identified so far due to the challenges of identifying partly digested prey. Moreover, it is not clear how well the identifiable diet reflects the unidentified portion. To address these issues, we DNA-barcoded unidentifiable fish items from the stomachs of 130 lionfish captured on Bahamian coral reefs. We identified 37 fish prey species, nearly half of which had not previously been recorded in this region. The total richness of lionfish prey fish recorded so far may represent up to ~54% of potential prey species on the study reefs. The relative importance of prey species in the visually identifiable diet portion, which was limited to 25% of prey items, differed from that in the ‘unidentifiable’ portion, which was largely resolved here with barcoding, weakening extra polations from visual identification. The high diet resolution afforded by barcoding can increase our ability to predict the impacts of invasive predators on recipient communities.
Gold nanoparticles induce apoptosis in MCF-7 human breast cancer cells.

BACKGROUND Gold nanoparticles have recently been investigated with respect to biocompatibility according to their interactions with cells. The purpose of this study was to examine cytotoxicity and apoptosis induction by well-characterized gold nanoparticles in human breast epithelial MCF-7 cells. METHODS Apoptosis was assessed by TUNEL, cytotoxicity by MTT assay and caspase 3, 9, p53, Bax and Bcl expression by real-time PCR assays. RESULTS Gold nanoparticles at up to 200 μg/mL for 24 hours exerted concentration-dependent cytotoxicity and significant upregulation of mRNA expression of p53, bax, caspase-3 and caspase-9, whereas expression of anti- apoptotic bcl-2 was down-regulated. CONCLUSION To the best of our knowledge this is the first report showing that gold nanoparticles induce apoptosis in MCF-7 cells via p53, bax/bcl-2 and caspase pathways.
Inorganic Nanostructures Decorated Graphene

The unique properties of graphene, a single-atom-thick sheet of sp2-hybridized carbon atoms with honeycomb structure, have ensured it is the subject of significant number of papers detailing its synthesis, properties and applications. In this chapter, the recent trends in the synthesis methodologies of graphene-inorganic nanostructures are presented and their impact on various applications reviewed.
Structural Damage Analysis of Masonry Walls using Computational Homogenization

This contribution deals with the application of computational homogenization techniques for structural masonry computations, as an alternative to the formulation of complex closed-form macroscopic constitutive laws. The complexity of modeling masonry material stems from the anisotropy evolution and localization induced by mesostructural damage. This phenomenon appears with preferential damage orientations, which are intimately related to the initial periodic structure of the material. The upscaling procedure used here relies on the formulation of mesoscopic constitutive laws at the level of the individual brick and mortar materials. A mesostructural unit cell with its corresponding periodicity requirements is used to deduce the average response of the masonry material through a scale transition. At the macroscopic scale, this averaged material response is used in the frame of an enhanced continuum approach with embedded localization bands, the widths of which are directly deduced from the initial periodicity of the material. The results obtained by the framework are illustrated and discussed by means of a structural computation example, which involves a complex cracking evolution together with fully anisotropic damage development.