Miracle-dz/UniFAR-FTUs huggingface dataset:
Re‐programming of translation following cell stress allows IRES‐mediated translation to predominate

There is now an overwhelming body of evidence to suggest that internal ribosome entry is required to maintain the expression of specific proteins during patho‐physiological situations when cap‐dependent translation is compromised, for example, following heat shock or during mitosis, hypoxia, differentiation and apoptosis. Translational profiling has been used by several groups to assess the extent to which alternative mechanisms of translation initiation selectively recruit mRNAs to polysomes during cell stress. The data from these studies have shown that under each condition 3–5% of coding mRNAs remain associated with the polysomes. Importantly, the genes identified in each of these studies do not show a significant amount of overlap, suggesting that 10–15% of all mRNAs have the capability for their initiation to occur via alternative mechanism(s).
R&D, export and investment decision: evidence from European firms

ABSTRACT This article provides an empirical investigation of the mechanism through which R&D influences export and tangible investment decisions. The analysis is based on a large representative and cross-country comparative sample of manufacturing firms across seven European countries. The novelty of this work lies in the three aspects. First, we expand the results on the R&D–export and R&D–investment relationships to a wide sample of cross-European firms. This differentiates from previous works based on single-country samples. Second, to the best of our knowledge, this study is the first in years which assess empirically the relationship between R&D and tangible investment at the micro level. Third, we control for endogeneity of R&D and simultaneity in firms’ decision whether to export and carry out tangible investment. The results of the analysis suggest that R&D positively affects export propensity and tangible investment. The results also reveal that neglecting endogeneity and simultaneity issues leads to underestimate the effect of R&D to both export and investment propensities.
Comparative structure analysis of tyrosine and valine residues in unprocessed silk fibroin (silk I) and in the processed silk fiber (silk II) from Bombyx mori using solid-state (13)C,(15)N, and (2)H NMR.

The solid-state (13)C CP-MAS NMR spectra of biosynthetically labeled [(13)C(alpha)]Tyr, [(13)C(beta)]Tyr, and [(13)C(alpha)]Val silk fibroin samples of Bombyx mori, in silk I (the solid-state structure before spinning) and silk II (the solid-state structure after spinning) forms, have been examined to gain insight into the conformational preferences of the semicrystalline regions. To establish the relationship between the primary structure of B. mori silk fibroin and the "local" structure, the conformation-dependent (13)C chemical shift contour plots for Tyr C(alpha), Tyr C(beta), and Val C(alpha) carbons were generated from the atomic coordinates of high-resolution crystal structures of 40 proteins and their characteristic (13)C isotropic NMR chemical shifts. From comparison of the observed Tyr C(alpha) and Tyr C(beta) chemical shifts with those predicted by the contour plots, there is strong evidence in favor of an antiparallel beta-sheet structure of the Tyr residues in the silk fibroin fibers. On the other hand, Tyr residues take a random coil conformation in the fibroin film with a silk I form. The Val residues are likely to assume a structure similar to those of Tyr residues in silk fiber and film. Solid-state (2)H NMR measurements of [3,3-(2)H(2)]Tyr-labeled B. mori silk fibroin indicate that the local mobility of the backbone and the C(alpha)-C(beta) bond is essentially "static" in both silk I and silk II forms. The orientation-dependent (i.e., parallel and perpendicular to the magnetic field) solid-state (15)N NMR spectra of biosynthetically labeled [(15)N]Tyr and [(15)N]Val silk fibers reveal the presence of highly oriented semicrystalline regions.
Hormone replacement therapy and glucose tolerance in EPIC‐Norfolk: a population‐based study

Hormone replacement therapy (HRT) can affect glucose homeostasis in postmenopausal women but it is unclear whether long‐term use is associated with changes in glucose tolerance. The objective was to examine the relationship of glycated haemoglobin (HbA1C) concentration with HRT use in non‐diabetic postmenopausal women.
Self-modeling based diagnosis of services over programmable networks

In this paper, we propose a multi-layer self-diagnosis framework for networking services within SDN and NFV environments. The framework encompasses three main contributions: 1) the definition of multi-layered templates to identify what to supervise while taking into account the physical, logical, virtual and service layers. These templates are also finer-granular, extendable and machine-readable; 2) a self-modeling module that takes as input these templates, instantiates them and generates on-the-fly the diagnosis model that includes the physical, logical, and the virtual dependencies of networking services; 3) a service-aware root-cause analysis module that takes into account the networking services' views and their underlying network resources observations within the aforementioned layers. We also present extensive simulations to prove the fully automated, finer granularity and reduced uncertainty of the root cause of networking services failures and their underlying network resources.
Latent tuberculosis infection among undergraduate medical students in Rio de Janeiro State, Brazil.

SETTING Five medical schools in three cities with different tuberculosis (TB) incidence rates in Rio de Janeiro State, Brazil. OBJECTIVE To estimate prevalence of and associated factors for latent tuberculosis infection (LTBI) among medical students. DESIGN A cross-sectional survey was conducted among undergraduate students in pre-clinical, early and late clinical years from schools in cities with low (28/100,000), intermediate (63/100,000) and high (114/100,000) TB incidence rates. Information on socio-demographic profile, previous BCG vaccination, potential TB exposure, co-morbidity and use of respiratory protective masks was obtained. A tuberculin skin test (TST) was performed using the Mantoux technique by an experienced professional. A positive TST, defined as induration > or = 10 mm, was considered LTBI. RESULTS LTBI prevalence was 6.9% (95%CI 5.4-8.6). In multivariate analysis, male sex (adjusted odds ratio [aOR] 1.8; 95% CI 1.1-3.0), late clinical years (aOR 1.9; 95% CI 1.01-3.5), intermediate TB incidence (aOR 4.3; 95% CI 1.3-14.6) and high TB incidence in the city of medical school (aOR 5.1; 95% CI 1.6-16.8) were significantly associated with LTBI. CONCLUSIONS The higher prevalence of LTBI in late clinical years suggests that medical students are at increased risk for nosocomial Mycobacterium tuberculosis infection. The implementation of a TB control program may be necessary in medical schools, particularly in cities with higher TB incidence.
Concern for others: A management attribute of excellent companies

This paper examines concern for others as an attribute prevalent and commonly engaged in by the most successful companies in Zimbabwe. The attribute has been identified to be, among others, characteristic of these excellent companies and it is possible that the success of these companies is partly attributable to this characteristic. Although it is likely that this attribute contributes to successful performance by companies that apply it, there is a possibility that the achievement of this could be most effective if all the attributes including those that are not discussed here are implemented collectively resulting in a synergistic effect. The singular application of this attribute, concern for others , should nevertheless, be tried by business practitioners because it could offer significant improvements to enterprises of all kinds.
Exploring the possibility of mechanical energy as a multipurpose evaluation function for learning of whole body dynamic motions

To optimize dynamic motions of a humanoid is difficult and any analytical method to optimize these motions has not been established. Motion learning is an effective way to improve motions which are difficult to be optimized analytically and there are many studies on it. However, in the past studies, evaluation functions of motion were dependent on motions and made by human for each motion. To solve the problem, we propose to use mechanical energy as an evaluation function in learning of whole body dynamic motions of a humanoid. In this paper, we tried to optimize forward rolling motion and smashing roof tiles motion as examples of whole body dynamic motions using mechanical energy as an evaluation function. By verifying effectiveness of the learning result, we indicates the possibility that mechanical energy could become a multipurpose evaluation function in learning of whole body dynamic motions of a humanoid.
Cloning of a Human UDP-N-Acetyl-α-d-Galactosamine:PolypeptideN-Acetylgalactosaminyltransferase That Complements Other GalNAc-Transferases in Complete O-Glycosylation of the MUC1 Tandem Repeat*

A fourth human UDP-GalNAc:polypeptideN-acetylgalactosaminyltransferase, designated GalNAc-T4, was cloned and expressed. The genomic organization of GalNAc-T4 is distinct from GalNAc-T1, -T2, and -T3, which contain multiple coding exons, in that the coding region is contained in a single exon. GalNAc-T4 was placed at human chromosome 12q21.3-q22 by in situ hybridization and linkage analysis. GalNAc-T4 expressed in Sf9 cells or in a stably transfected Chinese hamster ovary cell line exhibited a unique acceptor substrate specificity. GalNAc-T4 transferred GalNAc to two sites in the MUC1 tandem repeat sequence (Ser in GVTSA and Thr in PDTR) using a 24-mer glycopeptide with GalNAc residues attached at sites utilized by GalNAc-T1, -T2, and -T3 (TAPPAHGVTSAPDTRPAPGSTAPPA, GalNAc attachment sites underlined). Furthermore, GalNAc-T4 showed the best kinetic properties with an O-glycosylation site in the P-selectin glycoprotein ligand-1 molecule. Northern analysis of human organs revealed a wide expression pattern. Immunohistology with a monoclonal antibody showed the expected Golgi-like localization in salivary glands. A single base polymorphism, G1516A (Val to Ile), was identified (allele frequency 34%). The function of GalNAc-T4 complements other GalNAc-transferases in O-glycosylation of MUC1 showing that glycosylation of MUC1 is a highly ordered process and changes in the repertoire or topology of GalNAc-transferases will result in altered pattern of O-glycan attachments.
Effect of torsional twist on 2nd order non-linear optical activity of anthracene and pyrene tricyanofuran derivatives.

Tricyanofuran (TCF) derivatives attached to both anthracene and pyrene moieties were synthesised and characterised by optical, electrochemical and computational techniques. Both compounds exhibited similar absorption profile as well as electrochemical behaviour, however the pyrene derivative showed 20-fold higher non-linear optical activity measured by the EFISH technique. This huge difference has been assigned to (i) a lower molar absorption and (ii) a higher torsion angle for the anthracene derivative, confirmed by both experimental X-ray diffraction and DFT calculations. Furthermore, we note that the μβ1. 907 value of -1700 × 10(-48) esu recorded for the pyrene derivative in CHCl3/pyridine is remarkable for a NLO chromophore lacking a classical push-pull structure.
Social and Distributional Effects of Public Transport Fares and Subsidy Policies: Case of Madrid, Spain

Despite the widespread implementation of urban transport subsidies in many cities, there are still only a limited evaluation of the equity of these policies and scarce quantitative assessment of their distributional incidence. This research contributes to filling this gap by developing a practical approach to evaluate the impact of fare subsidization on vertical equity. This paper implements a two-step methodology. First, two main indicators were developed to measure the social impact of the travel pass, a highly subsidized fare, to determine the effectiveness of the policy in reaching lower-income citizens. Second, by using the latest disaggregated data from a transportation survey, in Madrid, Spain, a multiple regression model revealed that travel pass usage (TPU) depended mainly on income level and accessibility to public transport. The results show that the accessibility level has a positive effect on the TPU indicator, whereas income level has a negative influence. Because income level is shown to play the most significant role in influencing public transport use, the subsidy policy associated with the travel pass in the city can be considered progressive, since it effectively targets economically disadvantaged groups. This fact suggests that subsidies for public transport in Madrid tend to favor vertical equity.
The Relationship between Animal Welfare and Economic Performance at Farm Level: A Quantitative Study of Danish Pig Producers

We propose a theoretical framework for the relationship between animal welfare and the economic performance of livestock farms. We empirically analyse this relationship based on a unique data set of randomly sampled Danish pig herds that includes information from unannounced inspections of the compliance with the animal welfare legislation. We find large variations in economic performance indicators and animal welfare indicators. The relationship between these two indicators is rather weak, but tends to be slightly positive. We conclude that management has a major influence on both economic performance and animal welfare so that good farm managers are able to obey all animal welfare regulations and, at the same time, achieve a high economic performance.
The impact of smoking status on 9.3 years incidence of cardiovascular and all-cause mortality among Iranian men

Abstract Aim: To examine the association of different smoking groups with cardiovascular disease (CVD), coronary heart diseases (CHD) and CVD attributed death and death due to all causes in a male Tehranian population. Methods: From a population-based study 3059 male individuals, aged ≥30 years, free of CVD at baseline were evaluated for a median of 9.3 years. The adjusted hazard ratios (HRs) for incident CVD/CHD, total and CVD mortality regarding their smoking status were calculated using Cox proportional regression analysis, considering never smoking as reference. Results: A total of 158 deaths, in which 78 were CVD attributable, occurred. Considering CVD and CHD events, this study identified 299 and 257 events, respectively. Being a past smoker significantly increased the risk of CVD events (HR = 2.42, CI = 1.28–0.56), however, it has no effect on CHD events, total and CVD mortality. Being a current smoker (more than 10 cigarettes a day) dramatically increased the risk of CVD/CHD events and total/CVD mortality. However, smoking less than 10 cigarettes per day only increased the risk of CVD (HR = 2.12, CI = 1.14–3.95) and its mortality (HR = 4.57, CI = 1.32–15.79). Conclusion: The findings indicate that smoking increases the risk of incident CVD/CHD, total and CVD mortality, particularly CVD mortality. These outcomes were attributable to the daily amount of cigarettes smoked. Past smokers still had higher risk for CVD events, which cessation may not reduce.
Social Networks Analysis in Discovering the Narrative Structure of Literary Fiction

In our paper we would like to make a cross-disciplinary leap and use the tools of network theory to understand and explore narrative structure in literary fiction, an approach that is still underestimated. However, the systems in fiction are sensitive to readers subjectivity and attention must to be paid to different methods of extracting networks. The project aims at investigating into different ways social interactions are read in texts by comparing networks produced by automated algorithms-natural language processing with those created by surveying more subjective human responses. Conversation networks from fiction have been already extracted by scientists, but the more general framework surrounding these interactions was missing. We propose several NLP methods for detecting interactions and test them against a range of human perceptions. In doing so, we have pointed to some limitations of using network analysis to test literary theory, e.g. interaction, which corresponds to the plot, does not form climax.
Superresolution microscopy reveals the three-dimensional organization of meiotic chromosome axes in intact Caenorhabditis elegans tissue

Significance Meiosis is the essential cell division process that generates haploid gametes from diploid precursor cells. It relies on a dramatic reorganization of chromosomes around a central axis, which establishes a platform for homologous pairing and its subsequent stabilization by the formation of a proteinaceous structure, the synaptonemal complex, and by physical linkages resulting from crossover recombination events. Despite their central role in regulating key meiotic events, little is known about the organization of the chromosome axes. Here, we use superresolution microscopy, combined with CRISPR/Cas9 genome editing, to build a three-dimensional model of the synapsed chromosome axis. Our data link axis structure to its functions in synapsis and regulation of partner choice during meiotic double-strand break repair. When cells enter meiosis, their chromosomes reorganize as linear arrays of chromatin loops anchored to a central axis. Meiotic chromosome axes form a platform for the assembly of the synaptonemal complex (SC) and play central roles in other meiotic processes, including homologous pairing, recombination, and chromosome segregation. However, little is known about the 3D organization of components within the axes, which include cohesin complexes and additional meiosis-specific proteins. Here, we investigate the molecular organization of meiotic chromosome axes in Caenorhabditis elegans through STORM (stochastic optical reconstruction microscopy) and PALM (photo-activated localization microscopy) superresolution imaging of intact germ-line tissue. By tagging one axis protein (HIM-3) with a photoconvertible fluorescent protein, we established a spatial reference for other components, which were localized using antibodies against epitope tags inserted by CRISPR/Cas9 genome editing. Using 3D averaging, we determined the position of all known components within synapsed chromosome axes to high spatial precision in three dimensions. We find that meiosis-specific HORMA domain proteins span a gap between cohesin complexes and the central region of the SC, consistent with their essential roles in SC assembly. Our data further suggest that the two different meiotic cohesin complexes are distinctly arranged within the axes: Although cohesin complexes containing the kleisin REC-8 protrude above and below the plane defined by the SC, complexes containing COH-3 or -4 kleisins form a central core, which may physically separate sister chromatids. This organization may help to explain the role of the chromosome axes in promoting interhomolog repair of meiotic double-strand breaks by inhibiting intersister repair.
The COP9 Signalosome Is Required for Light-Dependent Timeless Degradation and Drosophila Clock Resetting

The ubiquitin–proteasome system plays a major role in the rhythmic accumulation and turnover of molecular clock components. In turn, these ∼24 h molecular rhythms drive circadian rhythms of behavior and physiology. In Drosophila, the ubiquitin–proteasome system also plays a critical role in light-dependent degradation of the clock protein Timeless (TIM), a key step in the entrainment of the molecular clocks to light–dark cycles. Here, we investigated the role of the COP9 signalosome (CSN), a general regulator of protein degradation, in fly circadian rhythms. We found that null mutations in the genes encoding the CSN4 and CSN5 subunits prevent normal TIM degradation by light in the pacemaker lateral neurons (LNs) as does LN-specific expression of a dominant-negative CSN5 transgene. These defects are accompanied by strong reductions in behavioral phase shifts of adult flies lacking normal CSN5 activity in LNs. Defects in TIM degradation and resetting of behavioral phases were rescued by overexpression of Jetlag (JET), the F-box protein required for light-mediated TIM degradation. Flies lacking normal CSN activity in all clock neurons are rhythmic in constant light, a phenotype previously associated with jet mutants. Together, these data indicate that JET and the CSN lie in a common pathway leading to light-dependent TIM degradation. Surprisingly, we found that manipulations that strongly inhibit CSN activity had minimal effects on circadian rhythms in constant darkness, indicating a specific role for the CSN in light-mediated TIM degradation.
Smoking Cessation Interventions among Hispanics in the United States: A Systematic Review and Mini Meta-Analysis

Purpose. The leading causes of mortality among Hispanics living in the United States are smoking related. This study sought to systematically review smoking cessation interventions targeting healthy Hispanic adults living in the United States, to conduct a “mini” meta-analysis of randomized controlled trials, and to offer recommendations for future research. Data Sources. Studies were identified through computerized bibliographic databases (PsychINFO, PsycARTICLES, PsycFirst, MEDLINE, Science Direct, and Dissertation Abstracts Online), article reference lists, conference abstracts, and unpublished data through October 2008. Study Inclusion and Exclusion Criteria. Evaluation of a smoking cessation intervention among healthy U.S. Hispanic adults. Studies included in the meta-analysis were also required to be randomized controlled trials. Data Extraction. Twelve studies were eligible for the systematic review and five studies for the meta-analysis. Two independent raters coded each study. Data Synthesis. Interventions consisted of self-help, nicotine replacement therapy, and community-based interventions, as well as individual, group, and telephone counseling. There was evidence for the efficacy of smoking cessation interventions at the end of treatment (odds ratio, 1.54; 95% confidence interval, 1.09–2.16), which was attenuated in the longer term. Conclusions. Tobacco use among U.S. Hispanics is a growing public health concern. Smoking cessation interventions demonstrate promise among Hispanic adults living in the United States. More randomized trials evaluating tobacco interventions in this special population are warranted, with examination of the effect of cultural specificity and acculturation.