Miracle-dz/UniFAR-FTUs huggingface dataset:
Kinetic characterization of the ATPase cycle of the DnaK molecular chaperone.

DnaK, the prototype Hsp70 protein of Escherichia coli, functions as a molecular chaperone in protein folding and protein disassembly reactions through cycles of polypeptide binding and release that are coupled to its intrinsic ATPase activity. To further our understanding of these processes, we sought to obtain a quantitative description of the basic ATPase cycle of DnaK. To this end, we have performed steady-state and pre-steady-state kinetics experiments and have determined rate constants corresponding to individual steps in the DnaK ATPase cycle at 25 degrees C. Hydrolysis of ATP proceeds very slowly with a rate constant (khyd approximately 0.02 min-1) at least 10-fold smaller than the rate constant for any other first-order step in the forward reaction pathway. The ATP hydrolysis step has an activation energy of 26.2 +/- 0.4 kcal/mol and is rate limiting in the steady-state under typical in vitro conditions. ATP binds with unusual strength to DnaK, with a measured KD approximately 1 nM. ADP binds considerably less tightly than ATP and dissociates from DnaK with a koff of approximately 0.4 min-1 (compared with a koff of approximately 0.008 min-1 for ATP). However, in the presence of physiologically relevant concentrations of inorganic phosphate (Pi), the release of ADP from DnaK is greatly slowed, approximately to the rate of ATP hydrolysis. Under these conditions, the ADP-bound form of DnaK, the form that binds substrate polypeptides most tightly, was found to represent a significant fraction of the DnaK population. The slowing of ADP release by exogenous Pi is due to thermodynamic coupling of the binding of the two ligands, which produces a coupling energy of approximately 1.6 kcal/mol. This result implies that product release is not strictly ordered. In the absence of exogenous inorganic phosphate, Pi product, by virtue of its higher koff, is released prior to ADP. However, at physiological concentrations of inorganic phosphate, the alternate product release pathway, whereby ADP dissociates from a ternary DnaK.ADP. Pi complex, becomes more prominent.
Treatment of human cancer with mumps virus

Based on confirmation that slightly toxic mumps virus having a strong affinity with mankind has a carcinostatic effect, it was used in cancer. As priority was put not on healing but on reconfirmation of its effectiveness against human cancer and on its mode of administration, patients mostly received only small amounts of the virus. Of 90 patients with terminal cancer of various kinds, treatment was assessed as very good in 37, and good in 42, excluding 11 patients who had been near death. Administration of mumps virus produced few side‐effects. The initial antineoplastic effect of the mumps virus therapy seemed to occur rapidly and strongly in proportion to swiftness in proliferation of cancer cells. Patients retaining physical strength often showed continuously suppressed growth of remaining tumors even after the disappearance of the initial effect, indicating a possible participation of tumor immunity in the present virus therapy.
Impact of Packetization and Scheduling on C-RAN Fronthaul Performance

Being considered as a key enabler for beyond 4G networks, Cloud-RAN (CRAN) offers advanced cooperation and coordinated processing capabilities and brings multiplexing gains. The high capacity and low latency fronthaul (FH) links requirement in the CRAN architecture can be reduced by a ? exible functional split of baseband processing between remote radio units (RRUs) and Baseband units (BBUs). Under the wide adoption of Ethernet in data centers and the core network, we consider the Radio over Ethernet (RoE) as an off- the-shelf alternative for FH link in this work. Moreover, the packetization process that packs each sample into Ethernet packets transported over the FH link will impact the CRAN performance. To this end, we investigate the impact of packetization on the proposed CRAN network and provide a packetization algorithm over the FH links. Furthermore, we also survey and analyze various packet scheduling policies applied at the aggregated RRU gateway in order to increase the multiplexing gain. Finally, the simulation results provide more in-depth insights on the potential multiplexing gains in terms of the maximum number of RRUs that can be supported over the Ethernet- based FH network.
Event-Radar: Real-time Local Event Detection System for Geo-Tagged Tweet Streams

The local event detection is to use posting messages with geotags on social networks to reveal the related ongoing events and their locations. Recent studies have demonstrated that the geo-tagged tweet stream serves as an unprecedentedly valuable source for local event detection. Nevertheless, how to effectively extract local events from large geo-tagged tweet streams in real time remains challenging. A robust and efficient cloud-based real-time local event detection software system would benefit various aspects in the real-life society, from shopping recommendation for customer service providers to disaster alarming for emergency departments. We use the preliminary research GeoBurst as a starting point, which proposed a novel method to detect local events. GeoBurst+ leverages a novel cross-modal authority measure to identify several pivots in the query window. Such pivots reveal different geo-topical activities and naturally attract related tweets to form candidate events. It further summarises the continuous stream and compares the candidates against the historical summaries to pinpoint truly interesting local events. We mainly implement a website demonstration system Event-Radar with an improved algorithm to show the real-time local events online for public interests. Better still, as the query window shifts, our method can update the event list with little time cost, thus achieving continuous monitoring of the stream.
Advances in the pulsed laser deposition of rare earth — Doped LiYF4 thin films on LiYF4 substrates

The influence of several experimental ablation/deposition parameters on the pulsed laser deposition on mono-crystalline LiYF<inf>4</inf> substrates of Nd<sup>3+</sup>:LiYF<inf>4</inf> thin films with suitable characteristics for their use as active medium in microlasers is investigated. The very first realization with the same technique of a Tm<sup>3+</sup>:LiYF<inf>4</inf> thin film on mono-crystalline LiYF<inf>4</inf> substrate is reported together with the first spectroscopic analysis of its optical properties.
Timing of changes in the entropy of the electroretinogram with Glaucoma

The photopic negative response (PhNR) component of the electroretinogram (ERG) is caused by loss of ganglion cells in the retina and can be used for the assessment of glaucoma. The purpose of this study was to use information theory techniques to explore subtle timing changes in the electroretinogram in glaucoma patients. The entropy of ERG signals were calculated within a sliding window, filtered with KZA filter to find the peaks and the timing of entropy changes determined. We found an earlier rise in entropy in glaucoma patients showing that this may have potential as new technique for glaucoma diagnosis.
Combined RF phase extraction and digitization

Phase-tracking problems are ubiquitous in communication systems, and the trend toward digital signal processing is rapidly necessitating the juxtaposition of RF phase-tracking and analog-to-digital conversion. A new technique for simultaneously performing these functions is presented. The approach offers the potential of reduced analog circuit complexity and cost over the traditional method of performing the functions separately. <<ETX>>
Effect of recombinant human activated protein C on the bactericidal activity of human monocytes and modulation of pro-inflammatory cytokines in the presence of antimicrobial agents.

OBJECTIVES To determine the effects of recombinant human activated protein C (rhAPC) on the antimicrobial activity and cytokine production of normal human monocyte-derived macrophages (MDMs) in the presence and absence of Escherichia coli infection, with and without treatment with levofloxacin or ampicillin. METHODS MDM monolayers were infected with E. coli ATCC 25922 and treated with levofloxacin or ampicillin in the presence or absence of rhAPC. Antimicrobial activity and cytokine (TNF-alpha, IL-1beta, IL-6 and IL-8) concentrations in the supernatants were measured. RESULTS When low concentrations of levofloxacin were used, a therapeutic concentration of rhAPC enhanced intracellular antibacterial activity at all time points. With ampicillin, antibacterial activity increased, was unaffected or diminished depending upon the drug concentration and assay time. Without antibiotics, rhAPC had no antibacterial effect. E. coli caused cytokine production to increase many fold. This increase was significantly greater with antibiotics (P < 0.01). Without antibiotics, rhAPC decreased production of TNF-alpha, IL-1beta and IL-6, but not IL-8. At high levofloxacin concentrations, rhAPC was associated with further increases in the concentrations of these cytokines. Cytokine concentrations at 24 h were unaffected by rhAPC in the presence of ampicillin and E. coli. CONCLUSIONS rhAPC can affect the bactericidal activity and cytokine production of human MDM in the presence of infection and antibiotic therapy. Importantly, factors such as type and concentration of antibiotics, presence of bacteria and timing must be taken into consideration when evaluating cytokine data from septic patients.
Managing corporate reputation,stakeholder relations and corporate social responsibility : a Southeast Asian perspective

The purpose of this paper is to identify the importance of and relationships between the three key concepts of corporate reputations and corporate social responsibility from a managerial perspective of corporate communication and corporate marketing. Despite the significant alignment and integration between corporate reputation, stakeholder relations and corporate social responsibility in their practice, all these have been managed as business duties (regulated), not genuinely for the substantial contribution to a larger social community and environment (self-regulated).
Time-optimal control of Takagi-Sugeno fuzzy systems

This paper presents a novel approach to synthesis of time-optimal control laws for the class of Takagi-Sugeno (TS) fuzzy systems. The idea is based on transforming the given TS system into a corresponding Piecewise Affine (PWA). The feedback strategy is derived by using the principles of Model Predictive Control (MPC). In order to cope with the state-dependent changes in process dynamics induced by fuzzy rules, we base the control design on PWA models with parametric uncertainties. The controller then utilizes on-line measurements of such uncertainties to provide the time-optimal control action.
Advanced Transceiver Algorithms for OFDM(A) Systems

With the increasing advancements in the digital technology, future wireless systems are promising to support higher data rates, higher mobile speeds, and wider coverage areas, among other features. While further technological developments allow systems to support higher computational complexity, lower power consumption, and employ larger memory units, other resources remain limited. One such resource, which is of great importance to wireless systems, is the available spectrum for radio communications. To be able to support high data rate wireless applications, there is a need for larger bandwidths in the spectrum. Since the spectrum cannot be expanded, studies have been concerned with fully utilizing the available spectrum. One approach to achieve this goal is to reuse the available spectrum through space, time, frequency, and code multiplexing techniques. Another approach is to optimize the transceiver design as to achieve the highest throughput over the used spectrum. From the physical layer perspective, there is a need for a highly flexible and efficient modulation technique to carry the communication signal. A multicarrier modulation technique known as orthogonal frequency division multiplexing (OFDM) is one example of such a technique. OFDM has been used in a number of current wireless standards such as wireless fidelity (WiFi) and worldwide interoperability for microwave access (WiMAX) standards by the Institute of Electrical and Electronics Engineers (IEEE), and has been proposed for future 4G technologies such as the long term evolution (LTE) and LTE-advanced standards by the 3rd Generation Partnership Project (3GPP), and the wireless world initiative new radio (WINNER) standard by the Information society technologies (IST). This is due to OFDM’s high spectral efficiency, resistance to narrow band interference, support for high data rates, adaptivity, and scalability.
Analytic expansion of the EEG lead field for realistic volume conductors

EEG forward calculation in realistic volume conductors using the boundary element method suffers from the fact that the solutions become inaccurate for superficial sources. Here we propose to correct an analytical approximation of the respective lead fields with series of spherical harmonics with respect to multiple expansion points. The necessary correction depends very much on the chosen analytical approximation. We constructed the latter such that the correction can be modelled adequately within the chosen basis. Simulations for a 3-shell prolate spheroid demonstrate the accurate modelling of the lead fields. Explicit comparison with analytically known solutions was done for the 3-shell spherical volume conductor showing that relative errors are mostly far below 1% even for the most superficial sources placed directly on the innermost surface.
Towards a Dutch Interoperability Framework

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Supplier selection in the Malaysian telecommunications industry

Supplier selection plays an important role in any organisation. This study investigates and examines supplier selection criteria and the impacts on buying firm performance; specifically focusing on the telecommunications industry in Malaysia. Previous studies in this area have focussed on the criteria such as quality, price, delivery, supplier relationship management and decision making tools and techniques. However, little research has been undertaken to include government policies and business ethics as elements in the supplier selection criteria. After a literature review and interviews, the development of conceptual frameworks and a number of hypotheses were put forward. To achieve an in-depth study, four major telecommunications service provider companies in Malaysia were chosen as a case study. A questionnaire was used as the main instrument in gathering data. The questionnaire is principally concerned with the understanding of supplier selection and its criteria; based upon the variables that are used for the model and hypothesis testing. The study also investigates the relationship between supplier selection and the impact of supplier selection to the company performance. This model was tested using Structural Equation Modelling (SEM) technique with the AMOS software application. Results indicated all of the criteria include quality, price, delivery, supplier relationship management, decision making tools and techniques, government policy and business ethics as valid constructs. A good model fit was also established. The findings of this research conclude that the influencing criteria to supplier selection in the telecommunications industry in Malaysia are supplier delivery performance standards and supplier relationship management even when considering the remainder of the above mentioned elements.The price factors were found to be less important due to this factor possibly leading to unhealthy competition in the market in terms of price wars among the suppliers in order to win the business. Most of the criteria are also interrelated to each other and affect the supplier selection decision. This model has added new perspectives to the study of supplier selection in the supply chain management field. As for future research it is suggested that intangible elements such as political, cultural and social influences are included in the conceptual framework; as this would offer important insight for management bodies in organisation, academia and public policy fields alike.
Ley Protective Autoimmunity in Atherosclerosis 431

This article is based on the 2015 Russell Ross Memorial Lecture in Vascular Biology presented at the American Heart Association’s Scientific Sessions Annual Conference, November 7 to 11, 2015, Orlando, FL. Atherosclerosis is an inflammatory disease of the arterial wall. This was first described by Rudolf Virchow, the founder of cellular pathology, in 1858, who viewed atheroma as the result of chronic inflammatory disease of the arterial intima. Electron microscopic evidence of monocyte association with atherosclerotic lesions was provided by Ross Gerrity. Russell Ross, who originally had proposed the response to injury model, focused on endothelial cell damage and smooth muscle proliferation, later became a key proponent of the inflammatory nature of atherosclerosis, and his work was instrumental in the widespread adoption of the inflammation hypothesis.
Influence of biotic and abiotic factors on annual aboveground biomass of an intermediate coastal marsh

Annual production of vegetation is an important indicator of various ecosystem processes in coastal marshes; many factors, both biotic and abiotic, can influence production of aboveground biomass. Using a 14-year data set, we evaluated the relative influence of 38 biotic and abiotic factors on annual aboveground biomass of an intermediate coastal marsh on the upper Gulf Coast of Texas. We used visual obstruction (VO) measurements as a surrogate variable in a prediction model to estimate available aboveground biomass in the marsh. Available biomass was greatest (3.34 kg/m2) when sampling site was flooded. Plant growth form, type of animal present, and composition of the ground cover influenced biomass of the marsh. Presence of insects was related to biomass (regression beta weight = 0.28), uniquely accounting for 7.6% of the incremental variance in biomass. The presence of moderate amounts of litter was also related to available biomass (beta weight = 0.86). Soil capping had little or no influence on aboveground biomass. Implementing standard protocols for long-term vegetation monitoring can be cost and time intensive. Our results suggest quantitative measurement of VO and qualitative observation of few variables (standing water, insects, and litter) measured annually can yield a reasonable assessment of aboveground biomass of intermediate coastal marshes.
Supply chain relationships between first and second tier suppliers in South African automotive supply chains : a focus on trust : original research

Background: The automotive industry is characterised by a network of long term collaborative agreements between component suppliers and assemblers. For collaboration to be successful trust between the supply chain partners is vital. Although many variables influence the efficiency of collaborative relationships, the human behaviour issues related to culture and trust are often much more difficult to solve. Trust is singled out in this article as a contributor to value in the supply chain relationships. Increased trust leads to more confidence resulting in better collaboration. Objectives: The purpose of the study was to explore collaborative relationships between first and second tier suppliers (component manufacturers) in automotive supply chains in South Africa and the role of trust in the relationship value. Method: This quantitative study had been conducted amongst members of the National Association for Automobile Component and Allied Manufacturers of South Africa (NAACAM), who are suppliers to the large automotive assemblers. The research utilised closed ended questions to obtain the perceptions of managers in automotive component manufacture regarding trust and relationship value with their best suppliers. Results: With a linear regression model it was found that there is a high degree of trust in the collaborative relationships between first and second tier suppliers of components in the automotive industry in South Africa. Conclusion: The automotive industry is one of the most successful industries in South Africa. They compete globally and apply best practices, particularly those in supply chain management. The industry is characterised by networks of collaborative relationships on different levels. These relationships seem to be successful as they are based on trust.
The Impact of Organizational Factors on Nurses Turnover Intention Behavior at Public Hospitals in Jordan: How Does Leadership, Career Advancement and Pay-Level Influence the Turnover Intention Behavior among Nurses

Background : Turnover intention has the possibility to have destructive effects on the life of nurses, healthcare organizations and societies in general. It is considered as one of the major problems that many organizations face, because higher costs and losses could happen because of turnover intention. All over the world, nurses shortage in increase. Organizational factors such as leadership, advancement opportunities, and pay level are among the most connected to the nurses turnover intention. Purpose : The purpose of the study is to explore the relationship between organizational factors (leadership, advancement opportunities, and pay level) and turnover intention. Design : Six hundred nurses working at public hospitals in Jordan, selected randomly, using survey method. Findings : This study revealed that there is a significant relationship between leadership and pay level on turnover intention of nurses and insignificant relationship between advancement opportunities and turnover intention among nurses. Conclusions : This study showed that organizational factors (leadership quality, pay level, and career advancement) are related to turnover intention. As such this study contributes to shedding a light on the activities of human resource management such as work conditions, stress management, and development of anti-turnover policies. This study therefore contributes to turnover intention literature in the context of the Middle East, particularly the healthcare industry is largely dependent on its nurses in enhancing healthcare services for individuals and in developing the industry.