Miracle-dz/UniFAR-FTUs huggingface dataset:
Differential Mechanisms of CRF1 and CRF2 Receptor Functions in the Amygdala in Pain-Related Synaptic Facilitation and Behavior

A major site of extrahypothalamic expression of corticotropin-releasing factor (CRF) and its G-protein-coupled CRF1 and CRF2 receptors is the amygdala, a key player in emotions and affective disorders. Pain-related plasticity in the laterocapsular division of the central nucleus of the amygdala (CeLC) generates emotional-affective responses and anxiety-like behavior. CRF1 receptor antagonists have anxiolytic effects. Although both CRF1 and CRF2 receptors couple positively to adenylyl cyclase, they can have opposite effects, but the underlying mechanism is unknown. This study addressed CRF1 and CRF2 receptor functions and mechanisms in the amygdala in a model of arthritic pain. Using whole-cell patch-clamp recordings of CeLC neurons, we found that a selective CRF1 receptor antagonist (NBI27914 [5-chloro-4-(N-(cyclopropyl)methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)]) amino-pyridine inhibited synaptic facilitation in brain slices from arthritic rats through a postsynaptic mechanism. Inhibition of the NMDA receptor-mediated synaptic component was occluded by a protein kinase A (PKA) inhibitor, consistent with our previous demonstration of PKA-dependent increased NMDA receptor function in arthritis pain-related plasticity. NBI27914 also decreased neuronal excitability through inhibition of highly tetraethylammonium (TEA)-sensitive ion channels that contribute to action potential repolarization and firing rate. In contrast, a CRF2 receptor antagonist (astressin-2B [cyclo(31–34) [d-Phe11,His12,CαMeLeu13,39, Nle17, Glu31, Lys34] Ac-Sauvagine(8–40)]) facilitated synaptic transmission through presynaptic inhibition of GABAergic transmission (disinhibition). NBI27914 inhibited arthritis pain-related behaviors (audible and ultrasonic vocalizations and hindlimb withdrawal reflexes). Astressin-2B had no significant behavioral effect. The data suggest that endogenous CRF1 receptor activation in the amygdala contributes to pain-related synaptic facilitation, increased excitability, and pain behavior through a postsynaptic mechanism involving activation of PKA and highly TEA-sensitive K+-currents. Presynaptic CRF2 receptor-mediated inhibition does not reach behavioral significance.
Radiation therapy induces the DNA damage response in peripheral blood

Stereotactic body radiation therapy (SBRT) is a radiotherapy modality that delivers highly conformal, ablative doses to a well-defined target. Here, using a semiquantitative multiplexed assay to analyze ATM and H2AX phosphorylation, we show that ATM kinase activity in peripheral blood mononuclear cells is induced following SBRT. This observation of a systemic ATM kinase-dependent DNA damage response in the peripheral blood is unprecedented and promotes the use of ATM serine-1981 phosphorylation as a predictive biomarker for DNA damaging modalities and ATM inhibitors.
Digital Reference in Slavic and East European Studies with an Examination of Practice at the University of Illinois and the Library of Congress

SUMMARY This article presents a survey of digital reference trends in the United States with an emphasis on services for Slavic and East European studies. It is based on the author's experience as a Slavic reference librarian at the University of Illinois (Slavic Reference Service) and the Library of Congress (European Division). Topics include the conflict between print and digital resources, coping with electronic serials, full-text databases and websites, digital communication tools such as e-mail, chat, and web forms, the proliferation of websites from Eastern Europe and the NIS, and opportunities for bibliographic instruction via the web. The article concludes with suggestions for keeping current and ideas for possible reference collaboration among Slavic and East European studies librarians.
“A Liberal Is Someone Who Has Not Been Mugged”: Criminal Victimization and Political Beliefs

An often‐repeated claim by conservative commentators attributes continuing liberal beliefs to the fact that progressives “have not been mugged. ” This claim thus portrays leftist views on public policy, including crime, as utopian, if not disingenuous—as held by people who have not had to face harsh realities. Using national‐level data from the General Social Survey that span two decades, we test this “mugging thesis. ” Controlling for an array of predictors of public opinion, we find no discernible relationship between being a crime victim and having a conservative worldview, support for conservative social policies, or punitiveness toward crime as measured by support for the death penalty and for harsher courts. These results question the validity of the “mugging thesis” and, more generally, of attempts to use slogans to undermine progressive political agendas.
Diagnostic Accuracy of Ber-EP4 for Metastatic Adenocarcinoma in Serous Effusions: A Meta-Analysis

Numerous studies have investigated the utility of Ber-EP4 in differentiating metastatic adenocarcinoma (MAC) from malignant epithelial mesothelioma (MM) and/or reactive mesothelial cells (RM) in serous effusions. However, the results remain controversial. The aim of this study is to determine the overall accuracy of Ber-EP4 in serous effusions for MAC through a meta-analysis of published studies. Publications addressing the accuracy of Ber-EP4 in the diagnosis of MAC were selected from the Pubmed, Embase and Cochrane Library. Data from selected studies were pooled to yield summary sensitivity, specificity, positive and negative likelihood ratio (LR), diagnostic odds ratio (DOR), and receiver operating characteristic (SROC) curve. Statistical analysis was performed by Meta-Disc 1.4 and STATA 12.0 softwares. 29 studies, based on 2646 patients, met the inclusion criteria and the summary estimating for Ber-EP4 in the diagnosis of MAC were: sensitivity 0.8 (95% CI: 0.78–0.82), specificity 0.94 (95% CI: 0.93–0.96), positive likelihood ratio (PLR) 12.72 (95% CI: 8.66–18.7), negative likelihood ratio (NLR) 0.18 (95% CI: 0.12–0.26) and diagnostic odds ratio 95.05 (95% CI: 57.26–157.77). The SROC curve indicated that the maximum joint sensitivity and specificity (Q-value) was 0.91; the area under the curve was 0.96. Our findings suggest that BER-EP4 may be a useful diagnostic adjunctive tool for confirming MAC in serous effusions.
Corporate and State Responses to Anti-Oil Protests in the Niger Delta

Conflicts between oil companies and village communities in the Niger Delta have lasted for several decades, but during the 1990s they escalated further and received international media coverage. Much of it focused on the anti-Shell protests by the Movement for the Survival of the Ogoni People (MOSOP) which led to Shell's withdrawal from the Ogoni area in 1993. Notwithstanding the political changes following General Abacha's death in June 1998, the conflicts are continuing. While the intensity of the Ogoni protests decreased from 1995 onwards, other ethnic and political groups across the Niger Delta began to disrupt oil activities. This article critically examines the response of the Nigerian state and the oil companies to the anti-oil protests in the Delta. The investigation focuses on three generic strategies: concessions by the state and oil companies to protesters, such as the creation of development projects; the use of public relations in dealing with the Niger Delta crisis; and the use of violence by the state and the oil companies against anti-oil protesters. The analysis suggests that the state and corporate response to the Niger Delta crisis has so far been inadequate in the sense that it fails to satisfy the demands of the local people. Judging from past experience, unless there are structural changes within Nigeria's institutional framework, which would allow for a more effective use of the country's oil wealth for the benefit of the oil-producing areas, conflicts in the Niger Delta are likely to continue.
AC breakdown characteristics of epoxy nanocomposites

Experiments were conducted to measure the ac breakdown strength of epoxy alumina nanocomposites with different filler loadings of 0.1, 1 and 5 wt%. The experiments were performed as per the ASTM D 149 standard on samples of thickness 0.5 mm, 1 mm and 3 mm in order to study the effect of thickness on the ac breakdown strength of epoxy nanocomposites. In the case of epoxy alumina nanocomposites it was observed that the ac breakdown strength was marginally lower for 0.1 wt% and 1 wt% filler loadings and then increased at 5 wt% filler loading as compared to the unfilled epoxy. The Weibull shape parameter (β) increased with the addition of nanoparticles to epoxy as well as with the increasing sample thickness for all the filler loadings considered. DSC analysis was done to study the material properties at the filler resin interface in order to understand the effect of the filler loading and thereby the influence of the interface on the ac breakdown strength of epoxy nanocomposites. It was also observed that the decrease in ac electric breakdown strength with an increase in sample thickness follows an inverse power-law dependence. In addition, the ac breakdown strength of epoxy silica nanocomposites have also been studied in order to understand the influence of the filler type on the breakdown strength.
The emergence of mathematizing as a culture of participation in the early childhood classroom

Abstract: This article investigates the practice of participation in a kindergarten classroom whose pedagogy in the learning of mathematics draws on the sociocultural perspective. The article illuminates collective negotiation processes constructed into being across classroom members. These joint negotiations demonstrate what it is to do and learn mathematics in this group. The primary data of this article consist of videotaped episodes of classroom interaction grounded in a daycare centre group comprising 11 children aged two- to four-years-old, an early childhood educator and a university researcher. The social interactions of the classroom were subjected to a qualitative micro-level analysis based on an applied sociolinguistic approach to language and discourse. The analysis illuminates social and pedagogical practices that support collective meaning making and emerging mathematics learning in the early education classroom. The results of the study suggest that young children are eager and able to participate in mathematical practices in settings that have institutionally-grounded pedagogical goals. However, individual efforts and interests in participation, while valued and encouraged by the pedagogical approach, can raise social conflicts that need to be resolved within the group. The role of the educator in guiding and supporting collective activity and supporting equal participation becomes crucial. This includes helping children learn to monitor and solve emerging participation conflicts via negotiation.
Institutionalization of risk and safety management at the local governmental level in Sweden

The starting point for this article is the need for empirical knowledge about organizational configuration for societal risk and safety management activities in a modern welfare society. In this paper, we use Sweden as an empirical frame to analyze the administrative management structure at the local governmental level. The analysis is based on statistical analysis of information from a web‐survey with administrative chief/head officials (n = 1283) with responsibilities for different municipal functions and sectors. The sample represented 25% of the Swedish municipalities (n = 290) and the response rate was approximately 60% (n = 766). The responses to two sets of questions (25 and 45 questions) are used for statistical analyses of management structures and task distribution within the municipal organizations. Principal component factor analyses with Varimax and Kaiser's Normalization was applied as a structure detection method. The results indicate a clear and uniform way to institutionalize societal risk and safety management at the local level. Furthermore, the management course of action is found to have different types of value characters. The implications that arise from the patterns identified in this study are considered to be of general relevance and topicality for research and practice in this area.
repeat variability Sequence-based estimation of minisatellite and microsatellite data

data Supplementary http://www.genome. org/cgi/content/full/gr.6554007/DC1 "Supplemental Research Data" References http://www.genome. org/cgi/content/abstract/gr.6554007v1#otherarticles Article cited in: P<P Published online October 31, 2007 in advance of the print journal. Open Access Freely available online through the Genome Research Open Access option. service Email alerting click here top right corner of the article or Receive free email alerts when new articles cite this article sign up in the box at the
Low-Dose Adenovirus Vaccine Encoding Chimeric Hepatitis B Virus Surface Antigen-Human Papillomavirus Type 16 E7 Proteins Induces Enhanced E7-Specific Antibody and Cytotoxic T-Cell Responses

ABSTRACT Induction of effective immune responses may help prevent cancer progression. Tumor-specific antigens, such as those of human papillomaviruses involved in cervical cancer, are targets with limited intrinsic immunogenicity. Here we show that immunization with low doses (106 infectious units/dose) of a recombinant human adenovirus type 5 encoding a fusion of the E7 oncoprotein of human papillomavirus type 16 to the carboxyl terminus of the surface antigen of hepatitis B virus (HBsAg) induces remarkable E7-specific humoral and cellular immune responses. The HBsAg/E7 fusion protein assembled efficiently into virus-like particles, which stimulated antibody responses against both carrier and foreign antigens, and evoked antigen-specific kill of an indicator cell population in vivo. Antibody and T-cell responses were significantly higher than those induced by a control adenovirus vector expressing wild-type E7. Such responses were not affected by preexisting immunity against either HBsAg or adenovirus. These data demonstrate that the presence of E7 on HBsAg particles does not interfere with particle secretion, as it occurs with bigger proteins fused to the C terminus of HBsAg, and results in enhancement of CD8+-mediated T-cell responses to E7. Thus, fusion to HBsAg is a convenient strategy for developing cervical cancer therapeutic vaccines, since it enhances the immunogenicity of E7 while turning it into an innocuous secreted fusion protein.
[An analysis of incidence trends and characteristics of soft tissue sarcoma in Beijing, 1999-2013].

Objective: To analyze the incidence trends and to describe the characteristics of soft tissue sarcoma (STS) among residents in Beijing from 1999 to 2013. Methods: Medical information of the cases diagnosed as STS(ICD10: C47&C49) from 1999 to 2013 in Beijing was extracted from the population-based database of Beijing Cancer Registry.Crude incidence rate, age-standardized incidence rates to Chinese population (ASRC)and the world population(ASRW) were calculated. The characteristics of the STS cases in different gender and age groups from urban and rural areas were respectively analyzed.The incidence trends and annual percentage changes (APC) during last 15 years were analyzed by using JoinPoint 3.4. 3 software. Results: A total of 2 048 cases were diagnosed as STS during the study period and the incidence rate of STS was 1.15 per 100 000 person-years. The ASRC was 0.74 per 100 000 person-years and ASRW was 0.86 per 100 000 person-years, respectively. Except for the impact of changes in the age composition, the APC of ASRW from 1999 to 2013 was 3.95%. For males, the incidence rate was increased from 0.65 per 100 000 in 1999 to 1.51 per 100 000 in 2013, and the APC was 4.27% (P<0.05); For females, the incidence rate was increased from 0.61 per 100 000 in 1999 to 0.91 per 100 000 in 2013, and the APC was 3.38% (P<0.05). In urban area, the incidence rate increased from 1.14 per 100 000 in 2000 to 1.35 per 100 000 in 2013, and the APC was 2.70% (P=0.05); In rural area, the incidence rate increased from 0.57 per 100 000 in 2000 to 0.98 per 100 000 in 2013, and the APC was 4.66% (P=0.04). 85.89%cases were pathologically diagnosed.Among them, undifferentiated pleomorphic sarcoma was the most common subtype, which accounting for 19.22%, followed by liposarcoma (19.04%), malignantnerve sheath tumor (10.18%), fibrosarcoma (10.06%)and sarcoma without definite type(7.22%). Conclusions: The incidence rate of STS is still increasing, especially in the rural area of Beijing. The most common subtypes of STS in different age-groups were different. Further study is needed to explore the high risks of STS in the population with specific characteristics.
Preserving privacy and fairness in peer-to-peer data integration

Peer-to-peer data integration - a.k. a. Peer Data Management Systems (PDMSs) - promises to extend the classical data integration approach to the Internet scale. Unfortunately, some challenges remain before realizing this promise. One of the biggest challenges is preserving the privacy of the exchanged data while passing through several intermediate peers. Another challenge is protecting the mappings used for data translation. Protecting the privacy without being unfair to any of the peers is yet a third challenge. This paper presents a novel query answering protocol in PDMSs to address these challenges. The protocol employs a technique based on noise selection and insertion to protect the query results, and a commutative encryption-based technique to protect the mappings and ensure fairness among peers. An extensive security analysis of the protocol shows that it is resilient to several possible types of attacks. We implemented the protocol within an established PDMS: the Hyperion system. We conducted an experimental study using real data from the healthcare domain. The results show that our protocol manages to achieve its privacy and fairness goals, while maintaining query processing time at the interactive level.
Dental Maturation in Children with Shunt-Treated Hydrocephalus

Objective The aim of this study was to examine the relationship between dental maturation and somatic development and to discover their possible deviations in children with shunt-treated hydrocephalus. Design Radiographs (orthopantomogram and hand-wrist radiograph) from children with shunt-treated hydrocephalus were analyzed with respect to the dental maturation and compared with values of an age- and sex-matched control group and population standards. Patients Forty-one children with shunt-treated hydrocephalus (27 boys and 14 girls) aged from 5 to 16 years (median age 11.0 years). Results The mean deviation of dental age from chronological age was significantly greater in the hydrocephalic group than in the control group. The mean value of dental age deviation was advanced in early pubertal stages but reduced in later pubertal stages. Conclusions These findings can be explained by changes in the endocrinological conditions, which are due to disturbed pituitary secretion. The present results could also partly reflect an adaptable developmental pathway in an otherwise strictly genetically determined process.
Size separation of circulatory DNA in maternal plasma permits ready detection of fetal DNA polymorphisms.

BACKGROUND Analysis of fetal DNA in maternal plasma has recently been introduced as a new method for noninvasive prenatal diagnosis, particularly for the analysis of fetal genetic traits, which are absent from the maternal genome, e.g., RHD or Y-chromosome-specific sequences. To date, the analysis of other fetal genetic traits has been more problematic because of the overwhelming presence of maternal DNA sequences in the circulation. We examined whether different biochemical properties can be discerned between fetal and maternal circulatory DNA. METHODS Plasma DNA was examined by agarose gel electrophoresis. The fractions of fetal and maternal DNA in size-fractionated fragments were assayed by real-time PCR. The determination of paternally and maternally inherited fetal genetic traits was examined by use of highly polymorphic chromosome-21-specific microsatellite markers. RESULTS Size fractionation of circulatory DNA indicated that the major portion of cell-free fetal DNA had an approximate molecular size of <0.3 kb, whereas maternally derived sequences were, on average, considerably larger than 1 kb. Analysis of size-fractionated DNA (</=0.3 kb) from maternal plasma samples facilitated the ready detection of paternally and maternally inherited microsatellite markers. CONCLUSIONS Circulatory fetal DNA can be enriched by size selection of fragment sizes less than approximately 0.3 kb. Such selection permits easier analysis of both paternally and maternally inherited DNA polymorphisms.
An Improved QIM-JPEG2000 Steganography and Its Evaluation by Steganalysis

This paper presents a modiﬁed QIM-JPEG2000 steganography which improves the previous JPEG2000 steganography using quantization index modulation (QIM). It does not increase the post-embedding ﬁle size, producing less degraded stego images. Steganalysis experiments show that the modiﬁed QIM-JPEG2000 is more secure than the previous QIM-JPEG2000 and is the most secure among major steganographic methods for JPEG2000 ever proposed.
Time coding output neurons in digital artificial neural networks

Previous research has proposed several platforms for the direct hardware implementation of neural networks. Most of these platforms employ a small number of special-purpose processors that emulate a given number of neurons. By contrast, this paper proposes a light-weight hardware model, called time coded output (TiCO) neuron, which codes its internal activation as the duration of its output pulses. Since the implementation of such a neuron requires only about 200 logical elements, standard state-of-the-art field-programmable gate arrays may host several hundreds of neurons on a single chip. Due to its output coding and its low footprint, TiCO neurons are particularly suited for the usage in embedded systems.
Parallel Algorithms for Degenerate and Weighted Sequences Derived from High Throughput Sequencing Technologies

Novel high throughput sequencing technologies have redefined the way genome sequencing is performed. They are able to produce millions of short sequences in a single experiment and with a much lower cost than previous methods. In this paper, we address the problem of efficiently mapping and classifying millions of degenerate and weighted sequences to a reference genome, based on whether they occur exactly once in the genome or not, and by taking into consideration probability scores. In particular, we design parallel algorithms for Massive Exact and Approximate Unique Pattern Matching for degenerate and weighted sequences derived from high throughput sequencing technologies.