Miracle-dz/UniFAR-FTUs huggingface dataset:
Alternative management techniques for the difficult airway: esophageal-tracheal Combitube

Purpose of review To summarize knowledge about the esophageal-tracheal Combitube in emergency medicine and anesthesia, with special emphasis on uncommon indications. Papers published between August 2003 and July 2004 are reviewed. Recent findings Tracheal intubation in the field is difficult and success rates are dependent upon training level (90-98%). Therefore, the esophageal-tracheal Combitube has been recommended for emergency ventilation after failed tracheal intubation with success rates of about 90%, even when performed by emergency medical technicians. Combitube insertion is easy to learn, but practice is necessary to retain skills. Use of this device has also been recommended for ventilatory support during routine anesthesia. Combitube ventilation is successful in over 96% of patients, with minimal trauma. Since only a small percentage of American anesthesiologists are familiar with the Combitube (<50%), using the device in anesthesia care may improve the skills required during emergency airway management. Blood gases are in the range of those obtained during endotracheal tube ventilation and aspiration prophylaxis is at least as good as that of the laryngeal mask airway. Successful uncommon uses of the device have also been recommended, for example, Combitube ventilation in craniomaxillomandibular surgery via the submental route and insertion of the device in a burns patient with minimal mouth opening and significant tracheal stenosis after long-term ventilation. Summary The esophageal-tracheal Combitube is a useful and efficient alternative airway characterized by high success rates in emergency situations. We recommend the use of a laryngoscope for insertion and strict adherence to the manufacturer's guidelines in order to maximize success and minimize potential injury.
The Rb-CDK4/6 Signaling Pathway Is Critical in Neural Precursor Cell Cycle Regulation*

The tumor suppressor, retinoblastoma (Rb), is involved in both terminal mitosis and neuronal differentiation. We hypothesized that activation of the Rb pathway would induce cell cycle arrest in primary neural precursor cells, independent of the proposed function of cyclin-dependent kinases 4/6 (CDK4/6) to sequester the CIP/KIP CDK inhibitors (CKIs) p21 and p27 from CDK2. We expressed dominant negative adenovirus mutants of CDKs 2, 4, and 6 (dnCDK2, dnCDK4, and dnCDK6) in neural progenitor cells derived from E12.5 wild type and Rb-deficient mouse embryos. In contrast to previous studies, our results demonstrate that in addition to dnCDK2, the dnCDK4/6 mutants can induce growth arrest. Moreover, the dnCDK4/6-mediated inhibition is Rb-dependent. The dnCDK2 partially inhibited cell growth in Rb-deficient cells, suggesting that CDK2 may have additional targets. A previously proposed function of CDK4/6 is CKI sequestration, thereby preventing the resulting inhibition of CDK2, believed to be the key regulator of cell cycle. However, our immunoprecipitations revealed that the dominant negative CDK mutants could arrest cell growth despite their interaction with p21 and p27. Taken together, our results demonstrate that both CDK2 and CDK4/6 are crucial for cell cycle regulation. Furthermore, our data underscore the importance of the Rb regulatory pathway in neuronal development and cell cycle regulation, independent of CKI sequestration.
High-brightness inverted InGaN-GaN multiple-quantum-well light-emitting diodes without a transparent conductive layer

Unlike the conventional layer structure of an InGaN-GaN multiple-quantum-well light-emitting diode (LED), an LED with reversed p-type and n-type layer sequence, and an n+/p+ tunnel junction has been investigated. When operated at 20 mA, the output power of the inverted LED is almost twice that of the conventional LED. Since the structures of these two LEDs are alike when analyzed by X-ray diffraction, the improvement in the light intensity could be attributed to the elimination of the absorption/reflection by the transparent conductive layer and/or some quality improvement of p-type GaN in the inverted LED.
Sports-related workload and injury risk: simply knowing the risks will not prevent injuries: Narrative review

Training loads contribute to sports injury risk but their mitigation has rarely been considered in a sports injury prevention framework. A key concept behind monitoring training loads for injury prevention is to screen for those at increased risk of injury so that workloads can be adjusted to minimise these risks. This review describes how advances in management of workload can be applied as a preventive measure. Primary prevention involves screening for preparticipation load risk factors, such as low training loads, prior to a training period or competition. Secondary prevention involves screening for workloads that are known to precede an injury developing so that modification can be undertaken to mitigate this risk. Tertiary prevention involves rehabilitation practices that include a graded return to training programme to reduce the risk of sustaining a subsequent injury. The association of training loads with injury incidence is now established. Prevention measures such as rule changes that affect the workload of an athlete are universal whereas those that address risk factors of an asymptomatic subgroup are more selective. Prevention measures, when implemented for asymptomatic individuals exhibiting possible injury risk factors, are indicated for an athlete at risk of developing a sports injury. Seven key indicated risks and associated prevention measures are proposed.
The prevalence of frontal variant frontotemporal dementia and the frontal lobe syndrome in a population based sample of 85 year olds

Objectives: To investigate the prevalence of the frontal lobe syndrome (FLS) and the frontal variant of frontotemporal dementia (fvFTD) in a population based sample of 85 year olds. Methods: A representative sample of 85 year olds (n = 451) in Gothenburg, Sweden was examined with a neuropsychiatric examination and a key informant interview performed by an experienced psychiatrist. A subsample underwent computed tomography (CT) of the head. The Lund-Manchester research criteria were used as a basis for a symptom algorithm to identify individuals with FLS and fvFTD. These were diagnosed blindly to the diagnosis of dementia according to DSM-III-R. Results: A total of 86 individuals (19%) fulfilled the criteria for FLS, and 14 of them fulfilled criteria for fvFTD. There were no differences between men and women. Among those with FLS, 75 (87%) fulfilled DSM-III-R criteria for other types of dementia, mainly Alzheimer's disease and vascular dementia. Among the 14 fvFTD cases, only five were demented according to DSM-III-R. Moderate to severe frontal atrophy was found in 93% of those with FLS (and in all cases with fvFTD), but also in 49% of those without FLS. FLS was found in 35% of those with moderate to severe frontal atrophy, and in 3% of those without these changes. Conclusions: The prevalence of fvFTD was 3% in 85 year olds, which is higher than previously expected in this age group. Only a minority of those with fvFTD were detected by the DSM-III-R criteria for dementia. FLS was even more common, especially in those diagnosed with a dementia disorder.
MiR-142-5p reverses the resistance to gefitinib through targeting HOXD8 in lung cancer cells.

OBJECTIVE To investigate the role and potential mechanism of micro ribonucleic acid (miR)-142-5p in the acquired resistance to gefitinib in lung cancer cells. MATERIALS AND METHODS The drug resistance of PC9/G cells was detected via methyl thiazolyl tetrazolium (MTT) assay. Expression levels of miR-142-5p and HOXD8 in PC9 and PC9/G cells were detected via quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) and Western blotting. PC9/G cells were transfected with miR-142-5p mimic, while PC9 cells were transfected with miR-142-5p inhibitor. Subsequently, expression changes of HOXD8 were determined using qRT-PCR and Western blotting, cell sensitivity to gefitinib was detected through MTT assay, and the apoptosis was detected via flow cytometry. Moreover, Dual-Luciferase reporter assay was conducted to determine the relationship between HOXD8 and miR-142-5p. Finally, potential involvement of HOXD8 in miR-142-5p-regulated gefitinib sensitivity was confirmed via rescue tests. RESULTS PC9/G cells were more significantly resistant to gefitinib compared with its parental PC9 cells. MiR-142-5p was down-regulated in PC9/G cells, while that of HOXD8 was up-regulated in PC9/G cells. Transfection of miR-142-5p mimic could inhibit the expression level of HOXD8 in PC9/G cells and reverse its resistance to gefitinib. Conversely, transfection of miR-142-5p inhibitor could upregulate HOXD8 in PC9 cells and promote its resistance to gefitinib. According to the Dual-Luciferase reporter assay, miR-142-5p could suppress the expression of HOXD8 through the targeted binding to the HOXD8 3'UTR. Moreover, miR-142-5p could regulate mitochondrial apoptosis pathway by targeting HOXD8. Finally, rescue tests confirmed that miR-142-5p regulated the sensitivity of PC9 cells to gefitinib by acting on the target gene HOXD8. CONCLUSIONS Down-regulation of miR-142-5p induces the resistance of lung cancer PC9 cells to gefitinib by upregulating HOXD8.
Tentative Characterization of New Environmental Giant Viruses by MALDI-TOF Mass Spectrometry

Objective: Metagenomic studies have revealed that Acanthamoeba polyphaga Mimivirus relatives are common in the environment; however, only three Acanthamoeba-growing giant viruses have been isolated from hundreds of environmental samples. We attempted herein to isolate new Acanthamoeba-growing giant viruses from environmental samples. Methods: We inoculated 105 environmental samples by our usual procedure but with the addition of selected antibiotics to inhibit bacterial overgrowth. Results: We isolated 19 giant viruses with capsid sizes of 150 to 600 nm, including one associated with a virophage. For the first time some were isolated from saltwater and soil samples. Tentative characterization using the PolB gene sequence was possible for some of these viruses. They were closely related to each other but different from the two previous isolates of Acanthamoeba polyphaga Mimivirus. Results obtained by MALDI-TOF MS analysis of viral particles were congruent with that of PolB sequencing. Conclusion: Our data confirm that Acanthamoeba-growing giant viruses are common in the environment. Additionally, MALDI-TOF MS analysis can be used for the initial screening of new viruses to avoid redundant analysis. However, due to their genetic variability, it is likely that the genome sequences of most of these viruses will have to be determined for accurate classification.
Deficient Antibody-Dependent Cellular Cytotoxicity against Human Immunodeficiency Virus (HIV)-Expressing Target Cells in Perinatal HIV Infection

ABSTRACT Peripheral blood mononuclear cells (PBMC) of human immunodeficiency virus (HIV)-infected children, age-matched HIV-seronegative controls, and HIV-infected asymptomatic and symptomatic adults were compared for their ability to mediate antibody-dependent cellular cytotoxicity (ADCC) and natural killer (NK) cell-mediated cytotoxicity against target cells expressing HIV or herpes simplex virus (HSV) antigens. Target cells consisted of CD4 lymphocytes purified from PBMC of HIV-seronegative adults and incubated with the IIIB strain of HIV, HUT78 cells chronically infected with IIIB, and HSV-infected human fibroblasts. PBMC of asymptomatic HIV-infected adults were generally able to lyse CD4 cells expressing HIV antigens. Direct correlation was found between the magnitude of lysis and absolute CD4 cell counts in these individuals. In contrast to these results, PBMC from HIV-infected children were generally unable to lyse IIIB-expressing CD4 cells, regardless of the children’s clinical status, age, or absolute CD4 cell counts. Cells from HIV-seronegative adults and children did not directly lyse these target cells either but, in contrast to cells of HIV-seropositive children, were able to mediate cell lysis when serum from an HIV-seropositive adult was added. However, effector cells from these HIV-infected children were able to mediate both ADCC against HSV-infected fibroblasts and NK cell-mediated cytotoxicity against IIIB-infected HUT78 cells. Reduced ability of PBMC from vertically HIV-infected children to mediate ADCC against HIV antigen-expressing CD4 cells may contribute to rapid progression to AIDS.
Reduction of a band-symmetric generalized eigenvalue problem

An algorithm is described for reducing the generalized eigenvalue problem Ax = &lgr;Bx to an ordinary problem, in case A and B are symmetric band matrices with B positive definite. If n is the order of the matrix and m the bandwidth, the matrices A and B are partitioned into m-by-m blocks; and the algorithm is described in terms of these blocks. The algorithm reduces the generalized problem to an ordinary eigenvalue problem for a symmetric band matrix C whose bandwidth is the same as A and B. The algorithm is similar to those of Rutishauser and Schwartz for the reduction of symmetric matrices to band form. The calculation of C requires order n2m operation. The round-off error in the calculation of C is of the same order as the sum of the errors at each of the n/m steps of the algorithm, the latter errors being largely determined by the condition of B with respect to inversion.
Lower extremity energy absorption and biomechanics during landing, part I: sagittal-plane energy absorption analyses.

CONTEXT Eccentric muscle actions of the lower extremity absorb kinetic energy during landing. Greater total sagittal-plane energy absorption (EA) during the initial impact phase (INI) of landing has been associated with landing biomechanics considered high risk for anterior cruciate ligament (ACL) injury. We do not know whether groups with different INI EA magnitudes exhibit meaningful differences in ACL-related landing biomechanics and whether INI EA might be useful to identify ACL injury-risk potential. OBJECTIVE To compare biomechanical factors associated with noncontact ACL injury among sagittal-plane INI EA groups and to determine whether an association exists between sex and sagittal-plane INI EA group assignment to evaluate the face validity of using sagittal-plane INI EA to identify ACL injury risk. DESIGN Descriptive laboratory study. SETTING Research laboratory. PATIENTS OR OTHER PARTICIPANTS A total of 82 (41 men, 41 women; age = 21.0 ± 2.4 years, height = 1.74 ± 0.10 m, mass = 70.3 ± 16.1 kg) healthy, physically active individuals volunteered. INTERVENTION(S) We assessed landing biomechanics using an electromagnetic motion-capture system and force plate during a double-legged jump-landing task. MAIN OUTCOME MEASURE(S) Total INI EA was used to group participants into high, moderate, and low tertiles. Sagittal- and frontal-plane knee kinematics; peak vertical and posterior ground reaction forces (GRFs); anterior tibial shear force; and internal hip extension, knee extension, and knee varus moments were identified and compared across groups using 1-way analyses of variance. We used a χ (2) analysis to compare male and female representation in the high and low groups. RESULTS The high group exhibited greater knee-extension moment and posterior GRFs than both the moderate (P < .05) and low (P < .05) groups and greater anterior tibial shear force than the low group (P < .05). No other group differences were noted. Women were not represented more than men in the high group (χ(2) = 1.20, P = . 27). CONCLUSIONS Greater sagittal-plane INI EA likely indicates greater ACL loading, but it does not appear to influence frontal-plane biomechanics related to ACL injury. Women were not more likely than men to demonstrate greater INI EA, suggesting that quantification of sagittal-plane INI EA alone is not sufficient to infer ACL injury-risk potential.
Evidence for an Anaplerotic/Malonyl-CoA Pathway in Pancreatic β-Cell Nutrient Signaling

A metabolic model of fuel sensing has been proposed in which malonyl-CoA and long-chain acyl-CoA esters may act as coupling factors in nutrient-induced insulin release (Prentki M, Vischer S, Glennon MC, Regazzi R, Deeney J, Corkey BE: Malonyl-CoA and long chain acyl-CoA esters as metabolic coupling factors in nutrient-induced insulin secretion. J Biol Chem 267:5802–5810, 1992). To gain further insight into the control of malonyl-CoA content in islet tissue, we have studied the short- and long-term regulation of acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) in the β-cell. These enzymes catalyze the formation of malonyl-CoA and its usage for de novo fatty acid biogenesis. ACC mRNA, protein, and enzymatic activity are present at appreciable levels in rat pancreatic islets and clonal β-cells (HIT cells). Glucose addition to HIT cells results in a marked increase in ACC activity that precedes the initiation of insulin release. Fasting does not modify the ACC content of islets, whereas it markedly downregulates that of lipogenic tissues. This indicates differential regulation of the ACC gene in lipogenic tissues and the islets of Langerhans. FAS is very poorly expressed in islet tissue, yet ACC is abundant. This demonstrates that the primary function of malonyl-CoA in the β-cells is to regulate fatty acid oxidation, not to serve as a substrate for fatty acid biosynthesis. The anaplerotic enzyme pyruvate carboxylase, which allows the replenishment of citric acid cycle intermediates needed for malonyl-CoA production via citrate, is abundant in islet tissue. Glucose causes an elevation in β (HIT)-cell citrate that precedes secretion, and only those nutrients that can elevate citrate induce effective insulin release. The results provide new evidence in support of the model and explain why malonyl-CoA rises markedly and rapidly in islets upon glucose stimulation: 1) glucose elevates citrate, the precursor of malonyl-CoA; 2) glucose enhances ACC enzymatic activity; and 3) malonyl-CoA is not diverted to lipids. The data suggest that ACC is a key enzyme in metabolic signal transduction of the β-cell and provide evidence for the concept that an anaplerotic/malonyl-CoA pathway is implicated in insulin secretion.
Mobile Posturography: Posturographic Analysis of Daily-Life Mobility

Objective Mobility is crucial to maintain a sufficient quality of life. Posturography should be therefore focused on the investigation of daily-life activities (“mobile posturography”). Nowadays, postural control is often estimated by stance tasks on a force plate under different sensorimotor conditions. This technique applies an indirect approximation of the center of body mass and is not related to tasks required for mobility. An alternative approach would be the direct measurement of body sway during daily-life conditions close to the center of body mass. The present study was aimed at investigating normal age-dependent postural control strategies by analyzing the body sway of male and female subjects in daily-life tasks. Furthermore, the results were compared with data of age- and sex-matched vestibular disorders to determine the sensitivity of the “mobile posturography”. Study Design Prospective study. Patients The patient group included 76 subjects, and the control group comprised a total of 246 healthy volunteers. Trunk sway measures were performed using the Vertiguard-D device. With the device fixed by a belt at the hip (center of body mass), the subjects had to undergo 14 daily-life tasks under different sensorimotor conditions. Ankle sway was determined during the sensory organization test (SOT) on the BalanceMaster force plate in all patients by estimating the center of body mass from center of pressure changes within the plantar area. Results A nonlinear relationship between age and body sway was observed in majority of all the conditions. Furthermore, large sex-related differences in body sway were observed. The sensitivity of the mobile posturography was higher than determined during the SOT-force plate measurements or reported in literature before for state-of-the-art platform posturography. Conclusion The present results indicate that the method introduced here can quantify postural deficits in a broad range and in an inhomogenous sample of patients.
A Pulsed Differential-Mode Current Injection Method for Electromagnetic Pulse Field Susceptibility Assessment of Antenna Systems

In this paper, a pulsed differential-mode current injection (PDMCI) method is proposed for radiated susceptibility assessment of antenna systems exposed to the harsh electromagnetic pulse (EMP) environment. Two types of coupling devices used for the injection and monitor of the high-power microwave and the wideband EMP, respectively, is designed based on the directional coupler. The coupling devices are applicable from kilohertz to gigahertz, by employing which the waveform distortion of the injected narrowband and wideband pulses can be avoided. To achieve the equivalence with the wideband EMP radiation, two methods are presented to obtain the waveforms of the equivalent injected voltages, when the reflection at input port of the equipment under test (EUT) is negligible or not. By using the linear extrapolation approach, the proposed method can achieve the equivalence with the high-level EMP radiation experiment, which is difficult to perform in the laboratory currently. This extrapolation method is also effective even if the response of the antenna system is nonlinear. By employing typical antenna systems with different nonlinear EUTs, the feasibility and the accuracy of the PDMCI method are verified.
The truth effect in relation to neuropsychological functioning in traumatic brain injury

Objectives: Individuals with traumatic brain injury may have difficulty accepting other viewpoints, but there is not yet a clinical test to measure the readiness with which one accepts new information as true. This study examined the truth effect—the tendency to rate previously seen material as being ‘more true’ than newly presented material—to explore whether individual differences in the truth effect can be quantified and related to memory or other neuropsychological variables, including brain injury severity. Methods: Seventy-four individuals being seen for neuropsychological assessment were asked to classify 15 semantic statements as biographical, scientific or historical and were later asked to rate 10 of the previously viewed statements and 10 new statements as either true or false. Results: A truth effect was clearly present in two groups which had gained familiarity with opposite sets of statements. As well, individuals with better immediate verbal memories rated more of the previously seen statements as true. Conclusions: A verbal encoding factor appears to underlie the truth effect and may explain the difficulties that some TBI patients have in accepting newly presented information as true.