Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of an unusual folding pattern in a catalytically active guanine quadruplex structure

In the presence of certain metal ions, DNA and RNA can form guanine quadruplex structures, which have been proposed to play a functional role in a variety of biological processes. An 18‐nucleotide DNA oligomer, PS2.M, d(GTG3TAG3CG3T2G2), was previously reported to bind hemin and the resulting complex exhibited peroxidase activity. It was proposed that PS2.M folds unimolecularly into an antiparallel quadruplex with unusual, single‐base loops and terminal guanines positioned in adjacent quartets. Here we describe structural and stability properties of PS2.M alone in different buffers and metal ions, using gel electrophoresis, circular dichroism (CD), ultraviolet (UV)‐visible spectroscopies, and one‐dimensional 1H nuclear magnetic resonance (NMR). Native gel behavior of PS2.M in the presence of either Na+ or Pb2+ suggests the formation of unimolecular structures but, in the presence of K+, both unimolecular and multistranded structures are observed. In the presence of Pb2+ ions, PS2.M forms a unimolecular quadruplex containing three guanine quartets. CD titrations reveal that binding of Pb2+ ions to PS2.M is stoichiometric, and a single lead cation suffices to fully fold PS2.M. The PS2.M–Na+ system also forms a similar unimolecular quadruplex. In the presence of K+, the PS2.M–K+ system forms mixed species. With increasing time and PS2.M concentration, the contribution of unimolecular species decreases while that of multimolecular species increases, and this behavior is independent of buffer media. These results suggest that the catalytically active form, studied in the presence of K+, may be a parallel, multistranded quadruplex rather than an antiparallel, unimolecular quadruplex. © 2006 Wiley Periodicals, Inc. Biopolymers 82:558–569, 2006
Neonatal rat hypoxia–ischemia: Effect of the anti‐oxidant mitoquinol, and S‐PBN

Background: The production of oxygen free radicals after perinatal hypoxia–ischemia is thought to play a critical role in the pathogenesis of the brain injury. Administration of anti‐oxidants may thus be neuroprotective. The aim of the present study was to investigate the effect of a mitochondria‐targeted anti‐oxidant mitoquinol (mitoQ) administered in the form of the prodrug mitoquinone, and an extracellular anti‐oxidant N‐tert‐butyl‐(2‐sulfophenyl)‐nitrone (S‐PBN; Aldrich, St Louis, MO, USA), on neuronal survival in the rat striatum after acute perinatal hypoxia–ischemia.
Schizophrenia and family rituals: measuring family rituals among schizophrenics and “normals”

Summary Family rituals consisting of ceremonial acts, traditions and patterned interactions serve as a basis of communication, collective identity, and stabilizing relationships. Therefore, the measurement of rituals in a schizophrenic family is an objectification of the family's symbolic communication and integration. Based upon this, the relationship between family rituals practice in 138 families both with a schizophrenic member (71) and without (67) was examined by the use of an originally developed instrument, the “Family Rituals Scale” (FRS). The psychometric properties of this instrument were tested and proved to be reliable. Families with a schizophrenic member were less ritualizing than the “normal” families. The correlation between FRS scores and Global Assessment Scale scores in schizophrenic patients was negative (the higher the psychosocial functioning level, the lower FRS scores), which means a higher performance of family rituals. The effect of other factors on FRS scores and the therapeutic implications are discussed.
How useful are the Adult Asperger Assessment and AQ-10 within an adult clinical population of all intellectual abilities?

Purpose – The Adult Asperger Assessment (AAA) was designed to be a screening tool to identify adults with Asperger syndrome and/or high-functioning autism. The AAA includes three questionnaires; the Autism Quotient (AQ), the Empathy Quotient (EQ) and the Relatives Questionnaire (RQ). The Autism Quotient-10 (AQ-10) was designed to be a “red flag” for healthcare professionals considering referral for ASD assessment. The purpose of this paper is to determine the usefulness of the AAA and AQ-10 as part of an adult autism diagnostic pathway that includes patients of all intellectual ability. Design/methodology/approach – Results were obtained for all patients who had received a clinical decision at Leeds Autism Diagnostic Service, which is a service that assesses patients of all intellectual ability, during 2015, n=214. Of these 132 were included in the analysis, 77 patients were excluded for not completing the AAA and four were excluded for not receiving a clinical decision. Findings – Results suggest that pa.
Standardization of the Griffith mental development scales for children aged 5～7 years in the city of Shanxi province

Objective To revise the Griffith mental development scales and establish the urban norm of Shanxi province. Methods The standardization samples consisted of 796 children aged 5 to 7 years were collected in a stratified and random way according to city rank, age, sex and paternal occupation. Each subject was tested individually and filled in a form. In the end, all the forms were analyzed applying in SPSS 7.5 For Windows. Results The total sample had better representation. The mean of GQ was about 100, and the distribution of GQ was normal. Item analysis indicated that the scale had suitable difficulty and highly discriminating function. The scale had high reliability and validity. Conclusion Griffith mental development scales have ideal reliability and validity. Apart from using the intelligent assessment in the Shanxi province, it can be used extensively through the country.
Interruption of the phosphoglucose isomerase gene results in glucose auxotrophy in Mycobacterium smegmatis

Two glycerol utilization mutants of Mycobacterium smegmatis that were unable to utilize most carbon sources except glucose were isolated. Supplementation of these media with small amounts of glucose restored growth in the mutants; these strains are therefore glucose auxotrophs. The mutant phenotype is complemented by the gene encoding phosphoglucose isomerase (pgi), and direct measurement of enzyme activities in the mutants suggests that this gene product is absent in the auxotrophic strains. Mapping of the mutant allele by Southern analysis demonstrates the presence of a 1-kb deletion extending into the coding sequence of pgi. The possible roles of phosphoglucose isomerase in mycobacterial cell wall synthesis and metabolic regulation are discussed.
CDC 2003 , To appear On quantized control and geometric optimization

This paper studies state quantization schemes for feedback stabilization of linear control systems with limited information. The focus is on designing the least destabilizing quantizer subject to a given information constraint. We explore several ways of measuring the destabilizing effect of a quantizer on the closed-loop system, including (but not limited to) the worst-case quantization error. In each case, we show how quantizer design can be naturally reduced to a version of the so-called multicenter problem from locational optimization. Algorithms for obtaining solutions to such problems, all in terms of suitable Voronoi quantizers, are discussed. In particular, an iterative solver is developed for a novel weighted multicenter problem which most accurately represents the least destabilizing quantizer design.
[The Vibrant Soundbridge System: a new kind of hearing aid for sensorineural hearing loss. 1: Function and initial clinical experiences].

INTRODUCTION Patients suffering from moderate to severe cochlear hearing impairment can not be considered for cochlear implantation on account of their relatively good residual hearing. Conventional hearing aids, on the other hand, have considerable disadvantages which clearly limit the benefit for these patients, e.g. feedback, sound distortion, unfavorable conditions for frequency transfer, occlusion, and recurrent infections of the auditory canal. In addition, many patients complain about a poor speech intelligibility in noise. VIBRANT SOUNDBRIDGE HEARING PROSTHESIS: Implantable hearing aids offer a new approach for improved auditory rehabilitation. The Vibrant Soundbridge system is based on an electromagnetic system, which is linked directly to the intact ossicular chain. Due to the high sound quality and the high frequency characteristic this system is ideally suited for the above-mentioned patient group. The usual disadvantages of conventional hearing aids can be avoided. Externally visible is merely the audio processor, which is worn in the retroauricular area and covered by hair. This processor transfers data and power via magnetic attachment transcutaneously. PATIENT POOL: During a European multicenter clinical study, 19 patients were implanted at MHH since February 1997. No significant complications occurred. RESULTS In all patients, postoperative unaided pure tone threshold was unaltered in comparison with the preoperative recordings. The use of the audio processor leads to a significant functional gain, particularly in the high frequencies. The patients report about undistorted hearing resulting in a better speech understanding even in situations with loud background noise. CONCLUSION The preliminary results show a promising new approach to the use of hearing prostheses for patients suffering from moderate to severe sensorineural hearing loss.
Biochemical identification of new proteins involved in splicing repression at the Drosophila P-element exonic splicing silencer

Splicing of the Drosophila P-element third intron (IVS3) is repressed in somatic tissues due to the function of an exonic splicing silencer (ESS) complex present on the 5′ exon RNA. Horan et al. isolated the silencer complex assembled in vitro and identified the proteins hrp36 and hrp38 and the cytoplasmic poly(A)-binding protein PABPC1 as novel functional components of the splicing silencer.
Brain Iron Quantification in Mild Traumatic Brain Injury: A Magnetic Field Correlation Study

BACKGROUND AND PURPOSE: Experimental studies have suggested a role for iron accumulation in the pathology of TBI. Magnetic field correlation MR imaging is sensitive to the presence of non-heme iron. The aims of this study are to 1) assess the presence, if any, and the extent of iron deposition in the deep gray matter and regional white matter of patients with mTBI by using MFC MR imaging; and 2) investigate the association of regional brain iron deposition with cognitive and behavioral performance of patients with mTBI. MATERIALS AND METHODS: We prospectively enrolled 28 patients with mTBI. Eighteen healthy subjects served as controls. The subjects were administered the Stroop color word test, the Verbal Fluency Task, and the Post-Concussion Symptoms Scale. The MR imaging protocol (on a 3T imager) consisted of conventional brain imaging and MFC sequences. After the calculation of parametric maps, MFC was measured by using a region of interest approach. MFC values across groups were compared by using analysis of covariance, and the relationship of MFC values and neuropsychological tests were evaluated by using Spearman correlations. RESULTS: Compared with controls, patients with mTBI demonstrated significant higher MFC values in the globus pallidus (P = .002) and in the thalamus (P = .036). In patients with mTBI, Stroop test scores were associated with the MFC value in frontal white matter (r = −0.38, P = .043). CONCLUSIONS: MFC values were significantly elevated in the thalamus and globus pallidus of patients with mTBI, suggesting increased accumulation of iron. This supports the hypothesis that deep gray matter is a site of injury in mTBI and suggests a possible role for iron accumulation in the pathophysiological events after mTBI.
Psychosocial Correlates of Hopelessness Among Men

This study examined overall hopelessness, explored differences in various variables (e.g. burnout) between men reporting no/mild hopelessness and moderate/severe hopelessness, and scrutinized factors associated with hopelessness. The study design was cross‐sectional, and the participants were 517 men randomly selected from the general population. About 12% of men reported moderate/severe hopelessness, and men with moderate/severe hopelessness were more often older, divorced/widowed, low educated, employed in blue‐collar positions, on sick leave/other/unemployment/retirement, and financially strained. They also reported more depression, posttraumatic symptoms, and burnout. Only unemployment/retirement, depression, and posttraumatic symptoms were independently associated with an increased risk for moderate/severe hopelessness in the regression analysis, with unemployment and retirement as the most important factors. In conclusion, this study seems to corroborate previous findings and may provide new insights into men's experiences and predictors of hopelessness. More research into the predictors of hopelessness in men is warranted.
Differential Expression of Carotenogenic Genes, Associated Changes on Astaxanthin Production and Photosynthesis Features Induced by JA in H. pluvialis

Haematococcus pluvialis is an organism that under certain conditions can produce astaxanthin, an economically important carotenoid. In this study, the transcriptional expression patterns of eight carotenogenic genes of H. pluvialis in response to jasmonic acid (JA) were evaluated using real-time PCR. Astaxanthin accumulation action and photosynthesis flourescence were monitored at the same time. The results showed all eight genes exhibited higher transcriptional expression significantly under JA treatments. JA25 (25 mg/L) induction had greater effect (>10-fold up-regulation) on the transcriptional expression of pds, crtR-B and lyc than on ipi-1, ipi-2, psy, bkt2, and crtO. JA50 (50 mg/L) treatment had greater impact on the transcriptional expression of ipi-1, ipi-2, psy, crtR-B and crtO than on pds, lyc and bkt2. Astaxanthin biosynthesis in the presence of JA appeared to be up-regulated mainly by psy, pds, crtR-B, lyc, bkt2 and crtO at the transcriptional level and ipi-1, ipi-2 at both transcriptional and post-transcriptional levels. Under JA induction, the photosynthetic efficiency [Y (II)] and the maximum quantum efficiency of PS II (Fv/Fm) decreased significantly, but the non-photochemical quenching of chlorophyll fluorescence (NPQ) increased drastically with the accumulation of astaxanthin.
Progressive perceptual audio rendering of complex scenes

Despite recent advances, including sound source clustering and perceptual auditory masking, high quality rendering of complex virtual scenes with thousands of sound sources remains a challenge. Two major bottlenecks appear as the scene complexity increases: the cost of clustering itself, and the cost of pre-mixing source signals within each cluster. In this paper, we first propose an improved hierarchical clustering algorithm that remains efficient for large numbers of sources and clusters while providing progressive refinement capabilities. We then present a lossy pre-mixing method based on a progressive representation of the input audio signals and the perceptual importance of each sound source. Our quality evaluation user tests indicate that the recently introduced audio saliency map is inappropriate for this task. Consequently we propose a "pinnacle", loudness-based metric, which gives the best results for a variety of target computing budgets. We also performed a perceptual pilot study which indicates that in audio-visual environments, it is better to allocate more clusters to visible sound sources. We propose a new clustering metric using this result. As a result of these three solutions, our system can provide high quality rendering of thousands of 3D-sound sources on a "gamer-style" PC.
Process of the development of T-2 toxin-induced apoptosis in the lymphoid organs of mice.

Female ICR:CD-1 mice orally treated with 10 mg/kg b.w. of T-2 toxin were killed at 1, 3, 6, 9, 12, 24 and 48 hr after treatment (HAT) and subjected to examination of the process of the development of T-2 toxin-induced apoptosis in the thymus and spleen. The early ultrastructural changes in lymphocytes characterized by shrinkage of the cell body and condensation of nuclear chromatin were detected at 3HAT in the thymus. The number of apoptotic lymphocytes observed by the in situ detection method for fragmented DNA increased drastically from 9 to 24 HAT in the thymus while it began to increase at 12 HAT in the spleen. The DNA ladder was first detected by agarose gel electrophoresis at 9 HAT and became clearer at 12 and 24 HAT in the thymus but was not clearly detected in the spleen throughout the observation period. Thus T-2 toxin-induced apoptosis developed earlier and was apparently severer in the thymus than in the spleen. Apoptotic was first detected by electron microscopy, then by the in situ detection method for fragmented DNA, and finally by DNA agarose gel electrophoresis.
Improving the Efficiency of IP Traceback at the DoS Victim

Denial of Service (DoS) attacks on Internet hosts and network components have been increasing in both frequency and enormity in the past few years causing great monetary damage to the attacked sites, as well as degradation in the browsing quality of Internet users. DoS attacks and its more damaging variant Distributed DoS (DDoS) attacks are also increasingly easy to cause with a host of tools like TFN, TFN2K and Trinoo, publicly available for everyone to download and use. The disturbing part is that the current IP protocol as is, provides little help in identifying the source of such attacks. To make things worse, it helps the attacker mask his real identity using what is known as IP Spoofing, wherein the source address of a packet is changed by the sender to a different one. Since there is no authentication of the IP source addresses at the IP layer, anyone can hide his/her identity behind such spoofed packets.
Efficacy of cryospray ablation for the treatment of chronic radiation proctitis in a pilot study

Radiation proctitis, a common condition associated with radiotherapy for the treatment of pelvic cancers, is characterized by difficult to manage rectal pain and bleeding. Cryotherapy is a novel technique, previously used in the treatment of vascular ectasias in the upper gastrointestinal tract. The aim of the present study was to determine the efficacy of cryospray application in the treatment of radiation proctitis.
Retinal Microvascular Abnormalities and Cognitive Impairment in Middle-Aged Persons: The Atherosclerosis Risk in Communities Study

Background and Purpose— Cerebral microvascular disease has been hypothesized to contribute to cognitive impairment, but few clinical data are available. Here, we examine the relation of retinal microvascular abnormalities with cognitive function in middle-aged persons free of stroke. Methods— The Atherosclerosis Risk in Communities Study is a population-based study with examinations every 3 years from 1987 through 1998. At visit 3, when participants were 51 to 70 years of age, retinal photographs were obtained and evaluated for retinal microvascular abnormalities according to standardized protocols. Cognitive function was assessed with standardized tests (Delayed Word Recall Test, Digit Symbol Subtest, and Word Fluency Test) at visits 2 and 4 and averaged for analysis. Persons with stroke or taking central nervous system–relevant medications were excluded, leaving 8734 with data for this study. Results— After education, diabetes mellitus, blood pressure, carotid intima-media thickness, and other risk factors were controlled for, retinopathy was associated with lower cognitive test scores. The adjusted odds ratios for persons with Delayed Word Recall scores 2 SD or lower than the mean were 2.60 [95% confidence interval (CI), 1.30 to 2.91] for any retinopathy, 3.00 (95% CI, 1.81 to 4.98) for microaneurysms, 3.39 (95% CI, 1.99 to 5.78) for retinal hemorrhage, and 3.07 (95% CI, 1.53 to 6.17) for soft exudates. Results were similar for the other 2 cognitive tests and in people with and without diabetes and hypertension. Conclusions— Retinopathy is independently associated with poorer cognitive function in middle-aged persons without stroke, suggesting that cerebral microvascular disease may contribute to the development of cognitive impairment.
Multi-stream long short-term memory neural network language model

Long Short-Term Memory (LSTM) neural networks are recurrent neural networks that contain memory units that can store contextual information from past inputs for arbitrary amounts of time. A typical LSTM neural network language model is trained by feeding an input sequence. i.e., a stream of words, to the input layer of the network and the output layer predicts the probability of the next word given the past inputs in the sequence. In this paper we introduce a multi-stream LSTM neural network language model where multiple asynchronous input sequences are fed to the network as parallel streams while predicting the output word sequence. For our experiments, we use a sub-word sequence in addition to a word sequence as the input streams, which allows joint training of the LSTM neural network language model using both information sources.
Transcriptome Analysis of Drosophila melanogaster Third Instar Larval Ring Glands Points to Novel Functions and Uncovers a Cytochrome p450 Required for Development

In Drosophila melanogaster larvae, the ring gland (RG) is a control center that orchestrates major developmental transitions. It is a composite organ, consisting of the prothoracic gland, the corpus allatum, and the corpora cardiaca, each of which synthesizes and secretes a different hormone. Until now, the RG’s broader developmental roles beyond endocrine secretion have not been explored. RNA sequencing and analysis of a new transcriptome resource from D. melanogaster wandering third instar larval RGs has provided a fascinating insight into the diversity of developmental signaling in this organ. We have found strong enrichment of expression of two gene pathways not previously associated with the RG: immune response and fatty acid metabolism. We have also uncovered strong expression for many uncharacterized genes. Additionally, RNA interference against RG-enriched cytochrome p450s Cyp6u1 and Cyp6g2 produced a lethal ecdysone deficiency and a juvenile hormone deficiency, respectively, flagging a critical role for these genes in hormone synthesis. This transcriptome provides a valuable new resource for investigation of roles played by the RG in governing insect development.
An Integrated Segmentation and Classification Approach Applied to Multiple Sclerosis Analysis

We present a novel multiscale approach that combines segmentation with classification to detect abnormal brain structures in medical imagery, and demonstrate its utility in detecting multiple sclerosis lesions in 3D MRI data. Our method uses segmentation to obtain a hierarchical decomposition of a multi-channel, anisotropic MRI scan. It then produces a rich set of features describing the segments in terms of intensity, shape, location, and neighborhood relations. These features are then fed into a decision tree-based classifier, trained with data labeled by experts, enabling the detection of lesions in all scales. Unlike common approaches that use voxel-by-voxel analysis, our system can utilize regional properties that are often important for characterizing abnormal brain structures. We provide experiments showing successful detections of lesions in both simulated and real MR images.