Miracle-dz/UniFAR-FTUs huggingface dataset:
Advanced characterizations of nanoparticles for drug delivery: investigating their properties through the techniques used in their evaluations

Abstract Nanomedicine has achieved a huge success in delivering a wide variety of drug molecules into the target site of the body. In this respect, the characterization of nanoformulation is very important to investigate the drug molecule together with its carrier as a nanoform during formulation, storage, and in vivo transport through the body. This review article summarizes important advanced characterization techniques of nanoformulation with respect to their theories, use of required instrumental parameters, sample preparation techniques, data interpretation, etc., to exploit them for the best possible results. This review article also sheds a glimpse to the shortcomings of these techniques together with further advancements required in future.
Learning spatial object localization from vision on a humanoid robot

We present a combined machine learning and computer vision approach for robots to localize objects. It allows our iCub humanoid to quickly learn to provide accurate 3D position estimates (in the centimetre range) of objects seen. Biologically inspired approaches, such as Artificial Neural Networks (ANN) and Genetic Programming (GP), are trained to provide these position estimates using the two cameras and the joint encoder readings. No camera calibration or explicit knowledge of the robot's kinematic model is needed. We find that ANN and GP are not just faster and have lower complexity than traditional techniques, but also learn without the need for extensive calibration procedures. In addition, the approach is localizing objects robustly, when placed in the robot's workspace at arbitrary positions, even while the robot is moving its torso, head and eyes.
Endocrinological Evaluations of a Neurofibromatosis Type 1 Cohort: Is it Necessary to Evaluate Autoimmune Thyroiditis in Neurofibromatosis Type 1?

Background: Neurofibromatosis type 1 is an autosomal dominant neurocutaneous disorder in which the coexistence of autoimmune thyroiditis and thyroid gland tumours has been reported previously. Aims: To determine the thyroid function and autoimmune thyroid diseases in neurofibromatosis type 1 patients in order to identify the possible association between neurofibromatosis type 1 and thyroid diseases. Study Design: Case-control study. Methods: The study includes 78 consecutive patients diagnosed with neurofibromatosis type 1 between June 2010 and June 2014 and 50 healthy controls. Baseline demographic data were generated from patient examination record forms, including age, sex, height, and weight, as well as levels of free triiodothyronine, free thyroxine, thyroid-stimulating hormone, anti-thyroid peroxidase and anti-thyroglobulin levels. Results: Mean age, sex, and body mass index were similar in both groups (p>0.05). The mean levels of free triiodothyronine, free thyroxine, and thyroid-stimulating hormone were not statistically different between the neurofibromatosis type 1 and control groups. Similarly, no statistically significant difference was observed between the neurofibromatosis type 1 and control groups for anti-thyroid peroxidase and anti-thyroglobulin positivity (2.5% vs 0%, p>0.05). Conclusion: Screening for autoimmune thyroid disease and thyroid function seems to be unnecessary in patients with neurofibromatosis type 1.
Energy Expenditure in Patients With Esophageal, Gastric, and Colorectal Cancer.

BACKGROUND Changes in resting energy expenditure (REE) appear to be one of the causes of nutritional depletion in cancer. Assessing REE may be an important tool for providing adequate nutritional therapy to these patients. The aims of this study were to evaluate REE of patients with gastrointestinal tract cancer and to compare it to that of healthy controls. METHODS A total of 20 patients, with esophageal (n = 3), gastric (n = 9), and colorectal (n = 8) cancers, and 20 healthy subjects were included. Indirect calorimetry (IC) was used to measure REE in both groups. The "pocket" equation (30 kcal/kg) and the Harris-Benedict equation, with correction factors of 1.3 (activity) and 1.1 (injury), were employed for assessment of the estimated total energy expenditure (TEE). Statistics included Mann-Whitney and paired t tests, Bland Altman analysis, and multivariate regression. RESULTS The REE of the patients (1,274.5 kcal [1,002.9-2,174.9]) was similar to that of the controls (1,445.5 kcal [1,114.5-1,762.6], not significant), even when corrected for the amount of metabolically active tissue. The pocket equation was effective in predicting the patients' TEE, with a 1.7% (32 kcal) difference being observed in comparison with the IC results corrected with the activity factor (not significant). CONCLUSIONS The patients with digestive tract cancers showed a similar REE to that of the controls. The current formula of 30 kcal/kg is suitable for estimating the TEE of these patients.
In Situ Scanning Probe Microscopy Nanomechanical Testing

Scanning probe microscopy (SPM) has undergone rapid advancements since its invention almost three decades ago. Applications have been extended from topographical imaging to the measurement of magnetic fields, frictional forces, electric potentials, capacitance, current flow, piezoelectric response and temperature (to name a few) of inorganic and organic materials, as well as biological entities. Here, we limit our focus to mechanical characterization by taking advantage of the unique imaging and force/displacement sensing capabilities of SPM. This article presents state-of-the-art in situ SPM nanomechanical testing methods spanning (1) probing the mechanical properties of individual one-dimensional nanostructures; (2) mapping local, nanoscale strain fields, fracture, and wear damage of nanostructured heterogeneous materials; and (3) measuring the interfacial strength of nanostructures. The article highlights several novel SPM nanomechanical testing methods, which are expected to lead to further advancements in nanoscale mechanical testing and instrumentation toward the exploration and fundamental understanding of mechanical property size effects in nanomaterials.
Insulin resistance in a large cohort of women with polycystic ovary syndrome: a comparison between euglycaemic-hyperinsulinaemic clamp and surrogate indexes

STUDY QUESTION Could surrogate indexes identify insulin resistant individuals among women with polycystic ovary syndrome (PCOS)? SUMMARY ANSWER Surrogate indexes may be able to rule in, but not rule out, insulin resistance in women with PCOS. WHAT IS KNOWN ALREADY Insulin resistance is a typical finding of women with PCOS and most clinical information on this issue is based upon surrogate indexes of insulin resistance. However, data on the performance of these indexes in PCOS women are very limited. STUDY DESIGN SIZE, DURATION A retrospective analysis of 406 women referred to our outpatient clinic for hyperandrogenism and/or menstrual dysfunction and submitted to hyperinsulinemic euglycaemic clamp between 1998 and 2015. PARTICIPANTS/MATERIALS, SETTING, METHODS In total, 375 of these women had PCOS by the Rotterdam criteria and were included in the study. Six surrogate indexes of insulin sensitivity were calculated from glucose and insulin levels, either at fasting (homeostasis model assessment (HOMA), glucose/insulin (G/I) ratio and quantitative insulin sensitivity check index (QUICKI)) or after oral glucose load (Gutt, Stumvoll0-120 and Matsuda). MAIN RESULTS AND THE ROLE OF CHANCE Overall, insulin resistance, as identified by the M-clamp value, was found in 74.9% of these women. The percentage was 59.3% in normal-weight vs 77.5% in overweight and 93.9% in obese subjects. All surrogate indexes were highly correlated with the M-clamp values. However, their ability to identify insulin resistant individuals was limited, in terms of sensitivity and especially in normal-weight subjects. ROC analysis showed similar performances of these indexes (AUC values 0.782-0.817). LIMITATIONS REASONS FOR CAUTION Potential referral bias of PCOS patients may have caused overestimation of the prevalence of insulin resistance in these women. WIDER IMPLICATIONS OF THE FINDINGS By using surrogate indexes many subjects with PCOS may be erroneously diagnosed as insulin sensitive, especially among normal-weight women. These indexes can be used to rule in, but not rule out, insulin resistance in PCOS. STUDY FUNDING/COMPETING INTEREST(S) Academic grants to P. Moghetti from the University of Verona. All authors declare no conflict of interest. TRIAL REGISTRATION NUMBER N/A.
Novel Orthopoxvirus Infection in an Alaska Resident

Summary A resident of interior Alaska, was diagnosed with an Orthopoxvirus infection. Phylogenetic analysis revealed it is a novel, previously undescribed Orthopoxvirus species. Phylogenetically, the virus is sister to recognized Old World orthopoxviruses, rather than North American Orthopoxvirus species.
Increasing Consumer Engagement by Tailoring a Public Reporting Website on the Quality of Diabetes Care: A Qualitative Study

Background The majority of health care utilization decisions in the United States are made by persons with multiple chronic conditions. Existing public reports of health system quality do not distinguish care for these persons and are often not used by the consumers they aim to reach. Objective Our goal was to determine if tailoring quality reports to persons with diabetes mellitus and co-occurring chronic conditions would increase user engagement with a website that publicly reports the quality of diabetes care. Methods We adapted an existing consumer-focused public reporting website using adult learning theory to display diabetes quality reports tailored to the user’s chronic condition profile. We conducted in-depth cognitive interviews with 20 individuals who either had diabetes and/or cared for someone with diabetes to assess the website. Interviews were audiotaped and transcribed, then analyzed using thematic content analysis. Results Three themes emerged that suggested increased engagement from tailoring the site to a user’s chronic conditions: ability to interact, relevance, and feeling empowered to act. Conclusions We conclude that tailoring can be used to improve public reporting sites for individuals with chronic conditions, ultimately allowing consumers to make more informed health care decisions.
Pressureless Bonding by Micro-Sized Silver Particle Paste for High-Temperature Electronic Packaging

Lead-free bonding in high-temperature electronic components is desirable for realizing eco-friendly technology. A pressureless process is more appropriate for electronic packaging because it enables a more automated manufacturing process and avoids any potential damage caused by application of pressure. Recently, Ag nanoparticles were used without pressure to join materials for high-temperature electronic applications. In this study, a Ag paste of micro-sized particles was proposed for electroless nickel immersion gold (ENIG) nished Cu pressureless bonding owing to its advantages of both cost effectiveness and easy manufacturing process compared to Ag nanoparticle paste. The micro-sized Ag paste was composed of both chestnut-burr-like (CBL) and spherical particles. The weight ratios of CBL to spherical particles were 10:0, 7:3, and 5:5. The bonding process was carried out at 573 K for 60 min in a nitrogen atmosphere. The experimental results showed that all of the sintered layers had an open porous structure. ENIGnished Cu joint using the Ag paste of the 5:5 weight ratio exhibited the shear strength of 18.6 MPa, which is comparable to that of a conventional Pb-5Sn joint. [doi:10.2320/matertrans.MD201513]
Rectangularization of the survival curve reconsidered: The maximum inner rectangle approach

Rectangularization of the survival curve—a key analytical framework in mortality research—relies on assumptions that have become partially obsolete in high-income countries due to mortality reductions among the oldest old. We propose refining the concept to adjust for recent and potential future mortality changes. Our framework, the ‘maximum inner rectangle approach’ (MIRA) considers two types of rectangularization. Outer rectangularization captures progress in mean lifespan relative to progress in maximum lifespan. Inner rectangularization captures progress in lifespan equality relative to progress in mean lifespan. Empirical applications show that both processes have generally increased since 1850. However, inner rectangularization has displayed country-specific patterns since the onset of sustained old-age mortality declines. Results from separating premature and old-age mortality, using the MIRA, suggest there has been a switch from reducing premature deaths to extending the premature age range; a shift potentially signalling a looming limit to the share of premature deaths.
Pharmacist participation in antimicrobial stewardship in Australian and French hospitals: a cross-sectional nationwide survey

Background Hospital pharmacists are an integral part of antimicrobial stewardship (AMS) programmes globally. Currently, little is known as to how hospital pharmacists see their role and involvement within the AMS framework. Objectives To assess the current level of involvement of Australian and French hospital pharmacists in AMS programmes and identify barriers limiting their involvement in AMS. Methods Hospital pharmacists throughout Australia and France were invited to participate in a nationwide online survey throughout March-May 2016. The survey was promoted through the national hospital pharmacists' association in Australia, while a stratified sampling method was used in France to invite pharmacists working in a variety of hospital settings. Results Invitations to participate in this survey were sent to 334 Australian pharmacists and 482 French pharmacists. Responses from 133 Australian and 126 French pharmacists were included for analysis. A total of 78.4% (203/259) of pharmacists reported the presence of an AMS programme. Pharmacists were most likely to be involved in AMS through assessing total antibiotic consumption and participating in AMS committee meetings. Barriers to participating in AMS included a lack of time and substantial non-clinical activities limiting involvement in AMS. Differences in responses were found between the two countries. Conclusions While the majority of pharmacists reported the presence of an AMS programme, multiple barriers to participation were identified by pharmacists in both countries. Further research should consider how to overcome the identified barriers to optimize the involvement of pharmacists in AMS.
Development and Application of a Novel Model System to Study “Active” and “Passive” Tumor Targeting

Macromolecular reagents can be targeted to tumors through active and passive mechanisms. “Active” targeting involves moieties, such as receptor ligands, to direct tumor cell binding, whereas “passive” targeting relies on long reagent circulating half-life, abnormal tumor vasculature, and poor lymphatic drainage for tumor entrapment. Here, we sought to study the impact of reagent circulating half-life on “active” and “passive” tumor uptake. The humanized prostate-specific membrane antigen (PSMA)-targeting antibody HuJ591 was used as the “active” targeting agent. HuJ591 was labeled with a Near Infrared (NIR) dye and its circulating half-life was modified by conjugation to high-molecular-weight Polyethylene Glycol (PEG). PEGylation did not negatively impact PSMA-binding specificity. “Active” and “passive” tumor targeting of intravenously injected antibody conjugates were then quantified by NIR fluorescent imaging of immunocompromised mice bearing bilateral isogenic PSMA-positive and PSMA-negative human tumor xenografts. Two isogenic tumor pairs were applied, PC3 ± PSMA (PC3-PIP/PC3-Flu) or LMD-MDA-MB-231 ± PSMA (LMD-PSMA/LMD). This study provided a unique model system to simultaneously observe “active” and “passive” tumor targeting within a single animal. “Passive” targeting was observed in all PSMA-negative tumors, and was not enhanced by increased HuJ591 size or extended circulating half-life. Interestingly, “active” targeting was only successful in some situations. Both PSMA-positive tumor models could be actively targeted with J591-IR800 and J591-PEG10K. However, the larger J591-PEG30K enhanced “active” targeting in the PC-3 tumor models, but inhibited “active” targeting the LMD-MDA-MB-231 tumor model. Successful “active” targeting was associated with higher PSMA expression. These results support the potential for “active” targeting to enhance overall macromolecular reagent uptake within tumors. Mol Cancer Ther; 15(10); 2541–50. ©2016 AACR.
The safety of a mixture of bupivacaine and lidocaine in children after urologic inguinal and scrotal surgery

Purpose Local anesthetic agents such as bupivacaine and lidocaine are commonly used after surgery for pain control. The aim of this prospective study was to evaluate the safety of a mixture of bupivacaine and lidocaine in children who underwent urologic inguinal and scrotal surgery. Materials and Methods Fifty-five patients who underwent pediatric urologic outpatient surgeries, were prospectively enrolled in this study. The patients were divided into three groups according to age (group I: under 2 years, group II: between 3−4 years, and group III: 5 years and above). Patients were further sub-divided into unilateral and bilateral groups. All patients were injected with a mixture of 0.5% bupivacaine and 2% lidocaine (2:1 volume ratio) at the surgical site, just before the surgery ended. Hemodynamic and electrocardiographic parameters were measured before local anesthesia, 30 minutes after administration of local anesthesia, and 60 minutes after administration. Results The patients' mean age was 40.5±39.9 months. All patients had normal hemodynamic and electrocardiographic parameters before local anesthesia, after 30 minutes, and after 60 minutes. Also, results of all intervals were within normal values, when analyzed by age and laterality. No mixture related adverse events (nausea, vomiting, pruritus, sedation, respiratory depression) or those related to electrocardiographic parameters (arrhythmias and asystole) were reported in any patients. Conclusions A mixture of bupivacaine and lidocaine can be safely used in children undergoing urologic inguinal and scrotal surgery. An appropriate dose has no clinically significant hemodynamic or cardiac changes and adverse effects.
Low Bandwidth and Low Power Video Encoding

ABSRACT In video compression, using reference frames next frames are represented with less number of bits. For this the reference frame has to be stored in eternal memory. Accessing reference frame while ME and MC, Large external memory bandwidth required this leads to increased system power dissipation and cost. By compressing the reference frame before storing to external memory can significantly reduces the memory bandwidth and power with minimal impact on quality. For this MMSQ-EC algorithm is used.
Prognosis of intervertebral disc loss from diagnosis of degenerative disc disease

Degenerative Disc Disease (DDD) is one of the most common causes of low back pain, and is a major factor in limiting the quality of life of an individual usually as they enter older stages of life, the disc degeneration reduces the shock absorption available which in turn causes pain. Disc loss is one of the central processes in the pathogenesis of DDD. In this study, we investigated whether the image texture features quantified from magnetic resonance imaging (MRI) could be appropriate markers for diagnosis of DDD and prognosis of inter-vertebral disc loss. The main objective is to use simple image based biomarkers to perform prognosis of spinal diseases using non-invasive procedures. Our results from 65 subjects proved the higher success rates of the combination marker compared to the individual markers and in the future, we will extend the study to other spine regions to allow prognosis and diagnosis of DDD for a wider region.
A Framework for Validation of Computer Models

We present a framework that enables computer model evaluation oriented toward answering the question: Does the computer model adequately represent reality? The proposed validation framework is a six-step procedure based on Bayesian and likelihood methodology. The Bayesian methodology is particularly well suited to treating the major issues associated with the validation process: quantifying multiple sources of error and uncertainty in computer models, combining multiple sources of information, and updating validation assessments as new information is acquired. Moreover, it allows inferential statements to be made about predictive error associated with model predictions in untested situations. The framework is implemented in a test bed example of resistance spot welding, to provide context for each of the six steps in the proposed validation process.
Analytic Modeling for Precise Speed Tracking of Multilink Robotic Fish

In this paper, a novel analytical-based kinematic and dynamic modeling and control approach is developed for reference speed tracking of a multijoint robotic fish. The kinematic model is improved by the introduction of input signal for controlling body undulation amplitude without distorting the model structure. Next, the dynamic model is developed in the discrete-time domain primarily because fish swimming pattern generates oscillating continuous thrust where the effect of thrust over one cycle of undulation can be calculated through averaging. Hence, the dynamics can be discretized according to the undulation rate. The novelty in developing the discrete-time dynamical model is three fold. First, the thrust gradient is derived with assistance of Lighthill's large amplitude elongated body theory. More importantly, affine-in-control dynamical model form, essential for designing a real-time model-based control algorithm, is derived by inclusion of the updated kinematic model. Second, the thrust delay is studied via pulse input response of the motion system, and incorporated in the dynamical model. Third, drag force coefficient calculation is also proposed by comparing the thrust model and empirically recorded steady-state speeds of the robotic fish. Finally, discrete-time model-based sliding-mode control (SMC) is designed for speed tracking. Experimental results verify that SMC based on the proposed dynamical model, can significantly improve speed tracking performance of a multilink robotic fish.