Miracle-dz/UniFAR-FTUs huggingface dataset:
Protein A‐tagging for purification of native macromolecular complexes from Candida albicans

Protein A‐tagging has become an important tool in characterization of protein–protein interactions in many systems, allowing purification of multicomponent complexes under native conditions. Here we provide a set of vectors that allow protein A‐tagging in Candida albicans, through addition of the tag to open reading frames. These vectors were successfully used to generate stably tagged proteins that were functional, shown to be localized appropriately or assembled into complexes. These new vectors comprise a useful addition to the C. albicans molecular toolbox. Copyright © 2003 John Wiley & Sons, Ltd.
16-QAM turbo equalization based on minimum mean squared error linear equalization

The turbo equalization principle, based on Berrou's (1993) turbo principle, is a high performance receiver scheme to mitigate intersymbol interference (ISI) caused by a frequency selective channel. Several turbo equalization (TEQ) schemes, like Douillard's (1995) MAP/MLSE TEQ, Laot's (1997) TEQ scheme by means of soft ISI cancellation and turbo equalization based on MMSE linear equalization have been presented different in performance and computational complexity. One of these schemes, turbo equalization based on MMSE linear equalization achieves nearly MAP/MLSE performance, while reducing the computational complexity tremendously. We extend this receiver scheme to 16-QAM and present simulation results for different ISI channels.
Adaptive clutter cancellation in bistatic radar

The detection performance of airborne bistatic radar depends on suitable clutter mitigation techniques. Space-time adaptive processing (STAP) is a candidate for suppressing bistatic clutter. Since STAP implementation relies on secondary data for covariance estimation, the nonstationary nature of typical bistatic angle-Doppler contours over range is a point of concern. In this paper we find that bistatic STAP suffers greater performance degradation than commonly observed in monostatic STAP analyses. Additionally we briefly examine approaches to enhance bistatic STAP capability.
A New Optimization Algorithm for the Vehicle Routing Problem with Time Windows

The vehicle routing problem with time windows VRPTW is a generalization of the vehicle routing problem where the service of a customer can begin within the time window defined by the earliest and the latest times when the customer will permit the start of service. In this paper, we present the development of a new optimization algorithm for its solution. The LP relaxation of the set partitioning formulation of the VRPTW is solved by column generation. Feasible columns are added as needed by solving a shortest path problem with time windows and capacity constraints using dynamic programming. The LP solution obtained generally provides an excellent lower bound that is used in a branch-and-bound algorithm to solve the integer set partitioning formulation. Our results indicate that this algorithm proved to be successful on a variety of practical sized benchmark VRPTW test problems. The algorithm was capable of optimally solving 100-customer problems. This problem size is six times larger than any reported to date by other published research.
Containment control for networked unknown Lagrangian systems with multiple dynamic leaders under a directed graph

In this paper, we address the containment control problem for multiple Lagrangian systems with multiple dynamic leaders in the presence of unknown nonlinearities and external disturbances under a directed graph. A distributed adaptive control algorithm with an adaptive gain design using both relative position and velocity feedback is proposed based on the approximation capability of neural networks. We present a necessary and sufficient condition on the directed graph such that the containment error can be reduced as small as desired. As a byproduct, we show a necessary and sufficient condition on leaderless consensus for networked Lagrangian systems under a directed graph with unknown nonlinearities and external disturbances, in which the systems achieve consensus asymptotically. We then propose a distributed containment control algorithm without using neighbors' velocity information.
Unreported antiretroviral use by HIV-1-infected participants enrolling in a prospective research study.

The concentration of viral RNA in plasma is the primary risk factor for sexual transmission of HIV-1 [1–3], and reductions in plasma HIV-1 RNA levels due to antiretroviral therapy (ART) result in marked decreases in HIV-1 transmission risk [4,5]. Results from studies of HIV-1 transmission and disease progression may be more difficult to interpret if a substantial proportion of HIV-1 infected partners have low or undetectable viral loads on ART, and thus, ART use at study enrollment is often an exclusion factor.
On Scott and Karp trees of uncountable models

Abstract Let and be two countable relational models of the same first order language. If the models are nonisomorphic, there is a unique countable ordinal α with the property that i.e. and are L∞ω-equivalent up to quantifier-rank α but not up to α + 1. In this paper we consider models and of cardinality ω1 and construct trees which have a similar relation to and as a above. For this purpose we introduce a new ordering T ≪ T′ of trees, which may have some independent interest of its own. It turns out that the above ordinal α has two qualities which coincide in countable models but will differ in uncountable models. Respectively, two kinds of trees emerge from α. We call them Scott trees and Karp trees, respectively. The definition and existence of these trees is based on an examination of the Ehrenfeucht game of length ω1 between and . We construct two models of power ω1 with mutually noncomparable Scott trees.
A minimum-time algorithm for intercepting an object on a conveyor belt

Purpose – The purpose of this paper is to provide a minimum time algorithm to intercept an object on a conveyor belt by a robotic manipulator. The goal is that the robot is able to intercept objects on a conveyor line moving at a given speed in minimum time.Design/methodology/approach – In order to formulate the problem, the robot and object‐arrival time functions were introduced, and conclude that the optimal point occurs at the intersection of these two functions. The search algorithm for finding the intersection point between the robot and object arrival time functions are also presented to find the optimal point in real‐time.Findings – Simulation results show that the presented algorithm is well established for various initial robot positions.Practical implications – A trapezoidal velocity profile was employed which is used in many industrial robots currently in use. Thus, it is believed that robot travel time algorithm is readily implemented for any commercially available robots.Originality/value – T.
Social Networking for Sharing Cloud Resources

Cloud computing is a model for enabling convenient, ubiquitous and on-demand network access to a shared pool of configurable computing resources (e.g. storage, applications, and networks) that can be provisioned with minimal management effort. Despite all these benefits, the sharing of resources with other users is a challenge, cloud providers do not commonly facilitate users in sharing their dedicated resources with others. In developing countries it is often too expensive for people to acquire a virtual machine of their own. Users may therefore wish to manage costs and increase computational resource usage by sharing their instances with others. Sadly it is not easy to do this at present. Social networks provide a structure that allows users to interact and share resources (e.g. pictures and videos) on the basis of a trustworthy relationship (e.g. Friendship). This paper highlights a Cloud Resource Bartering model (CRB-model) for sharing user's computational resources through a social network. In our approach we have linked a social network with the computational cloud to create a social cloud (SC) so that users can share their part of the cloud with their social community. A prototype system has been deployed on a social network by using the bartering resource trading mechanism. It is anticipated that this may help users to share their dedicated resources without the need for money changing hands and different communities.
The role oF neeD For CogniTive Closure in reTrieval-inDuCeD ForgeTTing anD misinFormaTion eFFeCTs in eyewiTness memory

The present research examined the role of the need for cognitive closure (NfCC) in the phenomenon of retrieval-induced forgetting (i.e., the forgetting of non-retrieved information induced by selective retrieval) and in subsequent post-event misinformation effects (i.e., the tendency to recall misleading post-event information in preference to originally presented items). In three experiments, it was shown that NfCC augments retrieval-induced forgetting which in turn magnifies misinformation effects in eyewitness situations. Overall, the present work highlights the crucial role of motivation in retrieval-induced forgetting and discusses the implications this has for eyewitness research.
De Que Colores: A Critical Examination of Multicultural Children's Books

The purpose of this paper was to examine children's books which highlighted multicultural issues, with the understanding that what is said about multiculturalism is as important, if not more so, than the simple fact that multiculturalism is discussed. We used an adaptation of Mulvey's (1975) “gaze,” to examine whiteness in seven multicultural children's books, and found that the white privilege in these texts positions people of color as non-American commodities and objects of a white gaze.
Critical protein domains and amino acid residues for gating the KIR6.2 channel by intracellular ATP

KATP channels couple intermediary metabolism to cellular excitability. Such a property relies on the inherent ATP‐sensing mechanism known to be located in the Kir6 subunit. However, the molecular basis for the ATP sensitivity remains unclear. Here we showed evidence for protein domains and amino acid residues essential for the channel gating by intracellular ATP. Chimerical channels were constructed using protein domains of Kir6.2 and Kir1.1, expressed in HEK293 cells, and studied in inside‐out patches. The N and C termini, although important, were inadequate for channel gating by intracellular ATP. Full ATP sensitivity also required M1 and M2 helices. Cytosolic portions of the M1 and M2 sequences were crucial, in which six amino acid residues were identified, i.e., Thr76, Met77, Ala161, Iso162, Leu164, and Cys166. Site‐specific mutation of any of them reduced the ATP sensitivity. Construction of these residues together with the N/C termini produced ATP sensitivity identical to the wild‐type channels. The requirement for specific membrane helices suggests that the Kir6.2 gating by ATP is not shared by even two closest relatives in the K+ channel family, although the general gating mechanisms involving membrane helices appear to be conserved in all K+ channels. J. Cell. Physiol. 198: 73–81, 2004. © 2003 Wiley‐Liss, Inc.
Increase in peripheral CD4 bright+ CD8 dull+ T cells in Parkinson disease.

BACKGROUND Immune abnormalities are known to be involved in the pathogenesis of sporadic Parkinson disease. OBJECTIVE To examine whether abnormalities in peripheral lymphocytes exist in Parkinson disease. METHODS Immune mediators, including CD1a, CD3, CD4, CD8, CD45RO, and Fas (CD95), were examined in peripheral lymphocytes of patients by 3-color flow cytometry. RESULTS Patients with Parkinson disease displayed a significantly greater population of circulating CD3+ CD4 bright+ CD8 dull+ lymphocytes than age-matched control subjects (P =.005) and patients with cerebrovascular disease (P =.002). The increase in these cells appeared to continue for at least 17 months. These T cells also expressed CD45RO and Fas, markers for activated T cells, while CD1a, a marker for thymic T cells, was negative, suggesting that these cells are mature T cells with immune activities. CONCLUSIONS As CD4+ CD8+ T cells are known to increase after some specific viral infections, the continuous increase in CD4 bright+ CD8 dull+ T cells shown here may indicate postinfectious immune abnormalities that are possibly associated with the pathogenesis of this slowly progressive, multifactorial neurodegenerative disease.
Energy-Fluctuated Multiscale Feature Learning With Deep ConvNet for Intelligent Spindle Bearing Fault Diagnosis

Considering various health conditions under varying operational conditions, the mining sensitive feature from the measured signals is still a great challenge for intelligent fault diagnosis of spindle bearings. This paper proposed a novel energy-fluctuated multiscale feature mining approach based on wavelet packet energy (WPE) image and deep convolutional network (ConvNet) for spindle bearing fault diagnosis. Different from the vector characteristics applied in intelligent diagnosis of spindle bearings, wavelet packet transform is first combined with phase space reconstruction to rebuild a 2-D WPE image of the frequency subspaces. This special image can reconstruct the local relationship of the WP nodes and hold the energy fluctuation of the measured signal. Then, the identifiable characteristics can be further learned by a special architecture of the deep ConvNet. Other than the traditional neural network architecture, to maintain the global and local information simultaneously, deep ConvNet combines the skipping layer with the last convolutional layer as the input of the multiscale layer. The comparisons of clustering distribution and classification accuracy with six other features show that the proposed feature mining approach is quite suitable for spindle bearing fault diagnosis with multiclass classification regardless of the load fluctuation.
Personal Data Access Based on Trust Assessment in Mobile Social Networking

Trustworthy personal data access control at a semi-trusted or distrusted Cloud Service Provider (CSP) is a practical issue although cloud computing has widely developed. Many existing solutions suffer from high computation and communication costs, and are impractical to deploy in reality due to usability issue. With the rapid growth and popularity of mobile social networking, trust relationships in different contexts can be assessed based on mobile social networking activities, behaviors and experiences. Obviously, such trust cues extracted from social networking are helpful in automatically managing personal data access at the cloud with sound usability. In this paper, we propose a scheme to secure personal data access at CSP according to trust assessed in mobile social networking. Security and performance evaluations show the efficiency and effectiveness of our scheme for practical adoption.
Whose Story Is It Anyway? Teaching Social Studies and Making Use of Kuwento

In the Filipino language, kuwento means “story,” but the concept itself encapsulates more than its literal meaning. Similar to talk story events in Hawaiian communities (Au & Jordan, 1981), kuwento serves as a tool to communicate everyday experiences within groups, especially among family and community members (Eugenio, 1981). It is an abstraction of history, congealing experience into a chain of events. It is what Bakhtin (1981) would call a unique speech experience—one that is shaped and developed in continuous and constant interaction with others. Kuwento is largely influenced by other people's words and ideas that eventually become incorporated into one's own. Like story and storytelling, kuwento takes many forms and can be used in the classroom during sharing time to construct and activate newer understandings (Cazden, 1994; Michaels, 1981). In the case of paucity in classroom material, the teacher can engage students to learn through her/his own writing (Vascellaro & Genishi, 1994) and her/his own construction of oral stories in different participant structures (Phillips, 1972). As we shall see in the case of Filipino Heritage Studies, the teacher's use of reflective- and real-time stories conveys the importance of history and present-day realities both in his and his students' lives. Although kuwento is also present in other participant structures, this article focuses on the teacher's whole-class lecture during a unit on the Philippine American War.
A Case Study: Middle School Boys’ Perceptions of Singing and Participation in Choir

The purpose of this intrinsic case study was to learn about the perceptions of singing and participation in choir of the author’s eighth grade choir students. Specific areas of focus included insight on why the eighth grade boys sing and enjoy singing, perceptions of singing in a daily choir class, and perceptions of singing in an auditioned after-school choir. Primary data collection techniques included one formal group interview with the five boys, informal field notes, concert programs, difficulty level of choral music, and the author’s personal insights and observations as the boys’ choir teacher and participant observer. Four primary themes emerged: singing, other people, Choralier Men, and silliness. This study cannot be generalized to the general population of eighth grade boys or middle school choir. However, other middle school choral teachers may recognize similarities between the author’s teaching practices and their own, and benefit from consideration of these findings.
TCPRand: Randomizing TCP payload size for TCP fairness in data center networks

As many-to-one traffic patterns prevail in data center networks, TCP flows often suffer from severe unfairness in sharing bottleneck bandwidth, which is known as the TCP outcast problem. The cause of the TCP outcast problem is the bursty packet losses by a drop-tail queue that triggers TCP timeouts and leads to decreasing the congestion window. This paper proposes TCPRand, a transport layer solution to TCP outcast. The main idea of TCPRand is the randomization of TCP payload size, which breaks synchronized packet arrivals between flows from different input ports. We investigate how TCPRand reduces consecutive packet drops and demonstrate various benefits of TCPRand with extensive experiments and ns-3 simulation. Our evaluation results show that TCPRand guarantees the superior enhancement of TCP fairness with negligible overheads in all of our test cases.
Reliability and Precision of the Mouse Calyx of Held Synapse

Traditionally, the calyx of Held synapse is viewed as a highly reliable relay in the sound localization circuit of the auditory brainstem, with every presynaptic action potential triggering a postsynaptic action potential in vivo. However, this view is at odds with slice recordings that report large short-term depression (STD). To investigate the reliability and precision of this synapse, we compared slice and in vivo recordings from medial nucleus of the trapezoid body neurons of young adult mice. We show that the extracellularly recorded complex waveform can be used to estimate both presynaptic release and postsynaptic excitability. Whereas under standard slice conditions the synapse underwent large STD, both extracellular and whole-cell recordings indicated that in vivo the size of the EPSPs was independent of recent history. The estimated quantal content was typically <20 in vivo, much lower than in the resting synapse under standard slice conditions. However, due to the large quantal size and summation of EPSPs, the safety factor of this synapse was generally still sufficiently large and postsynaptic failures were observed only infrequently in vivo. When present, failures were typically due to stochastic fluctuations in EPSP size or postsynaptic spike depression. In vivo, the calyx of Held synapse thus functions as a tonic synapse. The price it pays for its low release probability is an increase in jitter and synaptic latency and occasional postsynaptic failures.
THE CONTRIBUTION OF SPACE SYNTAX TO A COMPREHENSIVE THEORY OF ENVIRONMENTAL PSYCHOLOGY

The study of the interrelationships of human mind and behavior with the physical environment may be referred to as “environmental psychology” (or “behavioral geography,” “the psychology of space and place,” etc.). In my talk, I review elements of a comprehensive theory of environmental psychology. The theoretical framework of space syntax holds promise as an important contributor to this comprehensive theory, especially when combined with the analysis of isovists. It provides a rich and diverse set of quantitative indices for characterizing places in many ways that are potentially relevant to a variety of psychological responses, including choosing routes while locomoting, orientation and disorientation, spatial knowledge acquisition, perceived spaciousness, privacy and social interaction, stress and fear, and aesthetic judgments. Space syntax also falls short in contributing to a comprehensive theory. It flirts with causal ambiguity in some cases, underplays the significance of metric spatial properties (including distance and direction), says nothing about individual and group differences in people’s responses to layouts, overlooks the relevance of the overall shape or “gestalt” of layout, largely discounts the role of the superficial appearance of the environment, and assigns no special significance to the vertical or 3D qualities of places. I illustrate these claims with examples from research by my colleagues and me, and suggest ways to improve space syntax’s contribution to a comprehensive theory of environmental psychology. Psychology and the Environment Human psychology emerges from the interaction of a brain and nervous system in a body within a social and physical world. To explain psychological acts—behaviors, thoughts, emotions—one must look not only inward at the mind but also outward at the world. That is, psychology depends in part on characteristics of the built and natural environments in which people live. The study of the role of the environment in human psychology goes by a variety of names. For the purpose of this essay, I refer to this study of mind-behavior-world by the general term “environmental psychology”; I might just as well use terms like “behavioral geography,” “cognitive geography,” or “the psychology of space and place. ” Of course, the interrelationship of
Dimensionality and construct validity of the Rosenberg self‐esteem scale within a sample of recidivistic prisoners

Purpose – This study aims to examine the construct validity and factor structure of the Rosenberg self-esteem scale (RSES) using a sample (n ¼ 312) of Polish prisoners incarcerated in Nowogard High Security Prison. Design/methodology/approach – The number of confirmatory factor analysis (CFA) models under investigation was limited to two by virtue of employing a much stricter and more rigorously sound methodological procedure in which item errors were prevented from correlating, as suggested by Brown. Findings – Confirmatory factor analyses indicated that the two-factor (positive and negative self-esteem) model provided a better fit for the RSES items than did the one-factor model. Originality/value – The results provide some initial support for the two-dimensional model that could possibly be measuring substantively separate factors within a prison sample, thus calling into question the one-factor solution of the RSES.