Miracle-dz/UniFAR-FTUs huggingface dataset:
Collective Leadership as a Mediator of the Relationship between Team Trust and Team Performance

The present study investigated the relationship between collective leadership, team trust, and team performance longitudinally and with the inclusion of a performance feedback loop. Collective leadership was hypothesized to mediate the relationship between team trust and team performance; however, this hypothesis was not supported. Additional analyses support the conceptualization of collective leadership as an emergent state because collective leadership density increased significantly across two time-points. Further hypothesis testing revealed performance feedback to influence subsequent levels of team trust.
Long distance polymerase chain reaction of ascites lymphoma cells aids diagnosis establishment of abdominal Burkitt's lymphoma and Burkitt-like lymphoma

Burkitt's lymphoma (BL) and its variants, including Burkitt-like lymphoma (BLL), can be difficult to distinguish morphologically and immunophenotypically from diffuse large B-cell lymphoma (DLBCL). Because the treatment of BL differs markedly from that of other types of non-Hodgkin's lymphoma (NHL), exact pathological diagnosis is crucial. We report 2 cases of patients with BL initially diagnosed as DLBCL. Due to clinical features typical of BL, long distance polymerase chain reaction (PCR) for detecting the t(8;14) translocation involving the c-myc proto-oncogene was performed on ascites lymphoma cells. Re-evaluation by a reference hematopathologist, together with long distance PCR in both patients revealing the t(8;14) translocation, resulted in diagnosis modification to BL and BLL. Consequently, the patients were treated with intense high-dose chemotherapy regimens including central nervous system (CNS) prophylaxis, and complete remission was initially achieved in both patients. Both BL and BLL should be considered in the differential diagnosis of DLBCL if abdominal spread with ascites and metabolic disorders are present. Long distance PCR analysis of ascites lymphoma cells for the detection of the BL-typical c-myc rearrangement offers a convenient and rapid possibility of diagnosis verification in atypical or borderline cases of BL.
Analysis of the Chlorophyll a Fluorescence Transient of Spirodela polyrhiza by JIP-Test

Chlorophyll a fluorescence has been used as non-invasive probe for stress physiology and environmental investigation. The changes of photosynthetic apparatus in Spirodela polyrrhiza was studied by using chlorophyll a fluorescence technique and JIP-test. During L-serine-induced senescence, photosynthetic performance index (PI), reaction center (RC) decreased gradually and reached zero on the 8th day of cultivation. However, the oxygen evolving complex (OEC) remained nearly 60% and the capacity of Hill reaction remained about 43%. JIP-test also offers a platform to analyze the energy flux in fronds.
An ECT/ERT dual-modality system based on an internal electrode sensor

Electrical capacitance tomography (ECT) and electrical resistance tomography (ERT) are the two most developed ET methods nowadays. ERT is suitable to measure the two-phase flow in which conductive medium is taken as the continuous phase, while ECT is suitable to measure the two-phase flow in which non-conducting medium is taken as the continuous phase. In order to broaden the measurement range, generally combing the both ECT and ERT modalities to form an ECT/ERT dual-modality system is used. Therefore, this paper presents a novel unified sensor which can operate in both ECT and ERT modality and a series of experiments for oil-water two phase flow measurement are studied based on this dual-modality sensor with the inner electrode. The results demonstrate that the dual-modality system can operate stably, flexibly, and expand the measurement range.
Effect of Aripiprazole Augmentation of Serotonin Reuptake Inhibitors or Clomipramine in Treatment-Resistant Obsessive-Compulsive Disorder: A Double-Blind, Placebo-Controlled Study

Based on the evidence that aripiprazole added to serotonin reuptake inhibitors (SRIs) or clomipramine in treatment-resistant obsessive-compulsive disorder (OCD) has reported promising results, the present 16-week, double-blind, randomized, placebo-controlled trial had the aim to explore the efficacy of aripiprazole add-on pharmacotherapy on clinical symptoms and cognitive functioning in a sample of treatment-resistant OCD patients receiving SRIs. After clinical and neurocognitive assessments, patients were randomly allocated to receive, in a double-blind design, 15 mg/d of aripiprazole or a placebo. A final sample of 30 patients completed the study. The results obtained indicate that aripiprazole added to stable SRI treatment substantially improved obsessive-compulsive symptoms as measured by changes on the Yale-Brown Obsessive Compulsive Scale total score and subscores (obsessions, P = 0.007; compulsions, P = 0.001; total score, P < 0.0001). Regarding cognitive functions, improvement was observed in some explored areas, such as attentional resistance to interference (Stroop score, P = 0.001) and executive functioning (perseverative errors, P = 0.015). The findings provide evidence that aripiprazole augmentation of SRIs/clomipramine treatment is well tolerated and may be proposed as an effective therapeutic strategy to improve outcome in treatment-resistant OCD.
Use of serum antibody and lysozyme levels for diagnosis of leprosy and tuberculosis

Active tuberculosis (TB) and leprosy are difficult to diagnose early because there are few organisms to detect and the specific immune response does not distinguish between active and inactive disease. We developed an immunoassay for lysozyme to see whether serum lysozyme levels could be used to identify individuals with clinical leprosy or TB. The immunoassay for lysozyme proved superior to standard enzyme assays that were less sensitive and reliable. The lysozyme assay was compared with assays for antibodies to Mycobacterium tuberculosis lipoarabinomannan (LAM) and M. leprae phenolic glycolipid-1. The sera tested were from Ethiopian leprosy (paucibacillary and multibacillary) and TB patients and from healthy Ethiopian and U.S. controls. The lysozyme assay was able to detect more of the individuals with TB (sensitivity, 100% for 19 patients) or leprosy (sensitivity, 86% for 36 patients) than either antibody assay. In particular, lysozyme levels were raised in a higher proportion of the paucibacillary leprosy patients (83% of 17), for whom the antibody assays were less sensitive; the LAM IgG and the phenolic glycolipid-1 IgM levels were raised in only 62 and 44% of 16 patients, respectively. The data suggest that lysozyme measurements may be useful in the diagnosis of mycobacterial infections and other chronic infectious granulomatoses.
HDL antielastase activity prevents smooth muscle cell anoikis, a potential new antiatherogenic property

Various studies using proteomic approaches have shown that HDL can carry many proteins other than its constitutive apolipoprotein A‐I (apoA‐I). Using mass spectrometry and Western blotting, we showed the presence of α1‐antitrypsin (AAT) (SERPINA1, serpin peptidase inhibitor, clade A, an elastase inhibitor) in HDL, isolated either by ultra‐centrifugation or by selected‐affinity immunosorption using an anti‐apoA‐I column. Furthermore, we report that HDL possesses potent antielastase activity. We further showed that only HDL but not LDL is able to bind AAT. HDL‐associated AAT was able to inhibit extracellular matrix degradation, cell detachment, and apoptosis induced by elastase in human vascular smooth muscle cells (VSMCs) and in mammary artery cultured ex vivo. Degradation of fibronectin by elastase used as a marker of pericellular proteolysis was prevented by addition of HDL. Elastase present in aortic abdominal aneurysm (AAA) thrombus samples was also able to induce apoptosis of VSMCs in culture. This phenomenon was prevented by addition of HDL but not of LDL. Finally, we report that the proportion of AAT in HDL isolated from patients with an AAAis decreased relative to that from matched control subjects, suggesting a reduced capacity of HDL to inhibit elastase in these patients. In conclusion, our data provide evidence of a new potential antiatherogenic property of HDL attributable to AAT and its antielastase activity. —Ortiz‐Munoz, G. , Houard, X. , Martin‐Ventura, J.‐L. , Ishida, B. Y. , Loyau, S. , Rossignol, P., Moreno, J.‐A. , Kane, J. P., Chalkley, R. J., Burlingame, A. L. , Michel, J.‐B. , Meilhac, O. High‐density lipoprotein antielastase activity prevents smooth muscle cell anoikis, a potential new antiatherogenic property. FASEB J. 23, 3129–3139 (2009). www.fasebj. org
Phosphorylation-Dependent Degradation of p300 by Doxorubicin-Activated p38 Mitogen-Activated Protein Kinase in Cardiac Cells

ABSTRACT p300 and CBP are general transcriptional coactivators implicated in different cellular processes, including regulation of the cell cycle, differentiation, tumorigenesis, and apoptosis. Posttranslational modifications such as phosphorylation are predicted to select a specific function of p300/CBP in these processes; however, the identification of the kinases that regulate p300/CBP activity in response to individual stimuli and the physiological significance of p300 phosphorylation have not been elucidated. Here we demonstrate that the cardiotoxic anticancer agent doxorubicin (adriamycin) induces the phosphorylation of p300 in primary neonatal cardiomyocytes. Hyperphosphorylation precedes the degradation of p300 and parallels apoptosis in response to doxorubicin. Doxorubicin-activated p38 kinases α and β associate with p300 and are implicated in the phosphorylation-mediated degradation of p300, as pharmacological blockade of p38 prevents p300 degradation. p38 phosphorylates p300 in vitro at both the N and C termini of the protein, and enforced activation of p38 by the constitutively active form of its upstream kinase (MKK6EE) triggers p300 degradation. These data support the conclusion that p38 mitogen-activated protein kinase regulates p300 protein stability and function in cardiomyocytes undergoing apoptosis in response to doxorubicin.
Connexin32 deficiency exacerbates carbon tetrachloride-induced hepatocellular injury and liver fibrosis in mice

Abstract Objective: Liver fibrosis results from the perpetuation of the normal wound healing response to several types of injury. Despite the wealth of knowledge regarding the involvement of intracellular and extracellular signaling pathways in liver fibrogenesis, information about the role of intercellular communication mediated by gap junctions is scarce. Methods: In this study, liver fibrosis was chemically induced by carbon tetrachloride in mice lacking connexin32, the major liver gap junction constituent. The manifestation of liver fibrosis was evaluated based on a series of read-outs, including collagen morphometric and mRNA analysis, oxidative stress, apoptotic, proliferative and inflammatory markers. Results: More pronounced liver damage and enhanced collagen deposition were observed in connexin32 knockout mice compared to wild-type animals in experimentally triggered induced liver fibrosis. No differences between both groups were noticed in apoptotic signaling nor in inflammation markers. However, connexin32 deficient mice displayed decreased catalase activity and increased malondialdehyde levels. Conclusion: These findings could suggest that connexin32-based signaling mediates tissue resistance against liver damage by the modulation of the antioxidant capacity. In turn, this could point to a role for connexin32 signaling as a therapeutic target in the treatment of liver fibrosis.
Structural Dynamics as a Contributor to Error-prone Replication by an RNA-dependent RNA Polymerase*

Background: The physical basis for polymerase fidelity remains unclear. Results: Molecular dynamics simulations, NMR, and pre-steady-state kinetics of a mutator polymerase reveal conformational states of the active site that serve as fidelity checkpoints. Conclusion: Protein dynamics, largely concealed by x-ray crystallography, govern incorporation fidelity. Significance: Strategies have been inspired for engineering (anti)mutator polymerases and attenuated viruses. RNA viruses encoding high- or low-fidelity RNA-dependent RNA polymerases (RdRp) are attenuated. The ability to predict residues of the RdRp required for faithful incorporation of nucleotides represents an essential step in any pipeline intended to exploit perturbed fidelity as the basis for rational design of vaccine candidates. We used x-ray crystallography, molecular dynamics simulations, NMR spectroscopy, and pre-steady-state kinetics to compare a mutator (H273R) RdRp from poliovirus to the wild-type (WT) enzyme. We show that the nucleotide-binding site toggles between the nucleotide binding-occluded and nucleotide binding-competent states. The conformational dynamics between these states were enhanced by binding to primed template RNA. For the WT, the occluded conformation was favored; for H273R, the competent conformation was favored. The resonance for Met-187 in our NMR spectra reported on the ability of the enzyme to check the correctness of the bound nucleotide. Kinetic experiments were consistent with the conformational dynamics contributing to the established pre-incorporation conformational change and fidelity checkpoint. For H273R, residues comprising the active site spent more time in the catalytically competent conformation and were more positively correlated than the WT. We propose that by linking the equilibrium between the binding-occluded and binding-competent conformations of the nucleotide-binding pocket and other active-site dynamics to the correctness of the bound nucleotide, faithful nucleotide incorporation is achieved. These studies underscore the need to apply multiple biophysical and biochemical approaches to the elucidation of the physical basis for polymerase fidelity.
The early origins hypothesis with an emphasis on growth rate in the first year of life and asthma: a prospective study in Chile

Background: There is uncertainty about the impact of the programming hypothesis in terms of nutritional status at birth, rate of growth in the first year of life, length of gestation, breast feeding, and episodes of illness on asthma. An analysis was therefore carried out to test this hypothesis. Methods: Data were collected on 1232 children born between 1974 and 1978 in a semi-rural area of Chile. Measurements at birth and growth in the first year of life were obtained from a birth registry and clinical notes. Information on asthma was collected using the European Community Respiratory Health Survey questionnaire. Sensitisation to eight allergens and bronchial hyperresponsiveness (BHR) to methacholine were determined. All other information was obtained using a questionnaire. Polytomous logistic analyses were carried out to explore the association of factors at birth and during the first year of life with asthma symptoms, atopy, and BHR. Results: Weight and length gain in the first year were positively associated with wheeze (odds ratio (OR) 1.004, 95% CI 1.001 to 1.007 and OR 1.11, 95% CI 0.98 to 1.25, respectively). A higher body mass index (BMI) at birth was protective in subjects reporting both wheeze and waking with breathlessness (OR 0.54, 95% CI 0.35 to 0.84). Length rate in tertiles divided by length at birth in tertiles was related to asthma symptoms (OR 1.68, 95% CI 1.19 to 2.37). Most other assessments were not associated with asthma. Conclusion: These results show promising but inconclusive evidence that a rapid rate of growth in length, especially in newborn infants of low length, might be involved in the aetiology of asthma.
Genetic Abnormalities of the Protein C System: Shared Risk Factors in Young Adults with Migraine with Aura and with Ischemic Stroke?

Migraine, particularly migraine with aura (MA), may be a risk factor for ischemic stroke (IS). The reasons for this association are unknown. We investigated the presence of genetic abnormalities of the protein C system in 83 MA patients, 31 IS patients, and 124 healthy controls, all aged under 45 years. We found an increased frequency of activated protein C resistance due to Arg506Gln factor V mutation, and of protein S deficiency in both disorders, with figures higher than those reported in the general population and significantly different from those found in controls. These prothrombotic genetic abnormalities may be shared risk factors in IS and MA, and may play a role in increasing the risk of cerebrovascular disease in migraineurs.
Real-time human movement mapping to a virtual environment

The development of Virtual Reality (VR) into consumer products has given new perspectives to many areas. This paper presents a method to enhance the VR experience by mapping real time body movements of a person to a virtual world such that the user can do basic interactions (move, lift, throw) with objects found in the virtual environment. This technique combines a Kinect controlled full body avatar with Oculus Rift head-mounted-display through the Unity game engine. Despite few failures in Kinect skeletal tracker when in sideways poses, movements were mapped with zero latency allowing the user to interact in real time.
Efficient NFV deployment in data center networks

NFV is a new network concept that moves network functions from network appliances to virtual machines. Users can customize their own services by setting ordered traffic paths through network function virtual machines they want. Though this idea is flexible and scalable, it brings additional growth in east-west traffic. In this paper, we focus on NFV deployment in data center networks. An user has multiple VMs in a data center and wants to have some network functions for these VMs. According to the user's requirements and traffic flows, we design a heuristic NFV deployment algorithm for VNF allocation, VNF placement and traffic dispatching. We set a traffic model and evaluate our algorithm by simulation. We also give some further research directions in this paper.
Identification of Substrates of Human Protein-tyrosine Phosphatase PTPN22*

Stimulation of mature T cells activates a downstream signaling cascade involving temporally and spatially regulated phosphorylation and dephosphorylation events mediated by protein-tyrosine kinases and phosphatases, respectively. PTPN22 (Lyp), a non-receptor protein-tyrosine phosphatase, is expressed exclusively in cells of hematopoietic origin, notably in T cells where it represses signaling through the T cell receptor. We used substrate trapping coupled with mass spectrometry-based peptide identification in an unbiased approach to identify physiological substrates of PTPN22. Several potential substrates were identified in lysates from pervanadate-stimulated Jurkat cells using PTPN22-D195A/C227S, an optimized substrate trap mutant of PTPN22. These included three novel PTPN22 substrates (Vav, CD3ϵ, and valosin containing protein) and two known substrates of PEP, the mouse homolog of PTPN22 (Lck and Zap70). T cell antigen receptor (TCR) ζ was also identified as a potential substrate in Jurkat lysates by direct immunoblotting. In vitro experiments with purified recombinant proteins demonstrated that PTPN22-D195A/C227S interacted directly with activated Lck, Zap70, and TCRζ, confirming the initial substrate trap results. Native PTPN22 dephosphorylated Lck and Zap70 at their activating tyrosine residues Tyr-394 and Tyr-493, respectively, but not at the regulatory tyrosines Tyr-505 (Lck) or Tyr-319 (Zap70). Native PTPN22 also dephosphorylated TCRζ in vitro and in cells, and its substrate trap variant co-immunoprecipitated with TCRζ when both were coexpressed in 293T cells, establishing TCRζ as a direct substrate of PTPN22.
[Development of novel self-healing and antibacterial resin composite containing microcapsules filled with polymerizable healing monomer].

OBJECTIVE To develop novel self- healing and antibacterial resin composite containing microcapsules filled with polymerizable healing monomer, and to measure its properties for further clinical application. METHODS Microcapsules filled with healing monomer of triethylene glycol dimethacrylate were synthesized according to methods introduced in the previous research. Microcapsules were added into novel resin composite containing nano-antibacterial silica fillers grafted with long chain alkyl quaternary ammonium at mass fractions of 0, 2.5%, 5.0%, 7.5% or 10.0%. A commercial resin composite (Tetric N-Ceram) was used as control. Flexural test was used to measure resin composite flexural strength and elastic modulus. The single edge V-notched beam method was used to measure fracture toughness and self-healing efficiency. Scanning electron microscope (SEM) was used to examine the fractured surface of selected specimen for investigation of fracture mechanisms. RESULTS The flexural strength and elastic modulus of the resin composite were (96.4 ± 14.3) MPa and (6.2 ± 1.1) GPa respectively after incorporation of microcapsules up to 7.5%, and no significant difference was found between the experimental group and the control group [(99.1 ± 11.9) MPa and (6.1 ± 1.1) GPa] (P>0.05). The self-healing efficiency of (66.8 ± 7.0)% and (79.3 ± 9.7)% were achieved for resin composite with microcapsule mass fractions at 7.5% and 10.0%. SEM image showed that irregular films covered the fractured surface. Conclusions This novel self-healing and antibacterial resin composite containing microcapsules filled with polymerizable healing monomer exhibited a promising self- healing ability, which enabled itself well up for combating bulk fracture and secondary caries in clinical application.
The role of relationship promoter in new product development collaboration

Abstract Since new product development (NPD) collaborations with external partners have become the next generation in NPD practices, relationship promoters have become especially relevant as key contributors to the success of this type of inter-organisational collaboration. To realise successful outcomes in NPD collaborations, partners face the challenge of effectively integrating the role of relationship promoter into their existing and essential relationships of trust. It is within this context that this paper examines the role of the relationship promoter, by analysing its moderating effect on the relationship between trust and the outcomes of NPD collaboration – including new product competitiveness and partner satisfaction. We test our hypotheses against survey data from 107 innovative firms involved in NPD collaboration with the help of relationship promoters. The results show that, while a non-significant influence can be reported about the presence of a relationship promoter in terms of partner satisfaction, the relationship promotor weakens the influence of trust on new product competitiveness, making trust less important when it comes to creating a competitive new product.