Miracle-dz/UniFAR-FTUs huggingface dataset:
RhoE Is Regulated by Cyclic AMP and Promotes Fusion of Human BeWo Choriocarcinoma Cells

Fusion of placental villous cytotrophoblasts with the overlying syncytiotrophoblast is essential for the maintenance of successful pregnancy, and disturbances in this process have been implicated in pathological conditions such as pre-eclampsia and intra-uterine growth retardation. In this study we examined the role of the Rho GTPase family member RhoE in trophoblast differentiation and fusion using the BeWo choriocarcinoma cell line, a model of villous cytotrophoblast fusion. Treatment of BeWo cells with the cell permeable cyclic AMP analogue dibutyryl cyclic AMP (dbcAMP) resulted in a strong upregulation of RhoE at 24h, coinciding with the onset of fusion. Using the protein kinase A (PKA)-specific cAMP analogue N6-phenyl-cAMP, and a specific inhibitor of PKA (14–22 amide, PKI), we found that upregulation of RhoE by cAMP was mediated through activation of PKA signalling. Silencing of RhoE expression by RNA interference resulted in a significant decrease in dbcAMP-induced fusion. However, expression of differentiation markers human chorionic gonadotrophin and placental alkaline phosphatase was unaffected by RhoE silencing. Finally, we found that RhoE upregulation by dbcAMP was significantly reduced under hypoxic conditions in which cell fusion is impaired. These results show that induction of RhoE by cAMP is mediated through PKA and promotes BeWo cell fusion but has no effect on functional differentiation, supporting evidence that these two processes may be controlled by separate or diverging pathways.
Reduction of DNA damage in peripheral lymphocytes of obese patients after bariatric surgery-mediated weight loss

Obesity is associated with several detrimental health consequences, among them an increased risk for development of cancer, and an overall elevated mortality. Multiple factors like hyperinsulinemia, chronic microinflammation and oxidative stress may be involved. The comet assay has been proven to be very sensitive for detection of DNA damage and has been used to explore the relationship between overweight/obesity and DNA damage, but results are controversial. Very few investigations have been performed to correlate weight loss of obese individuals and possible reduction of DNA damage and these studies have not provided clear results. As currently, only surgical interventions (metabolic/bariatric surgery) enable substantial and sustained weight loss in the vast majority of morbidly obese patients, we analyzed whole blood samples of 56 subsequent patients prior, 6 and 12 months after bariatric surgery. No reduction of DNA damage was observed in comet assay analysis after 6 months despite efficient weight loss, but a significant reduction was observed 12 months after surgery. Concurrently, the ferric-reducing antioxidant power assay showed a significant reduction after 6 and 12 months. The level of oxidised glutathione and lipid peroxidation products were increased at 6 months but normalised at 12 months after surgery. As conclusion, a significant weight reduction in obese patients may help to diminish existing DNA damage besides improving many other health aspects in these patients.
Improving access to medicines via the Health Impact Fund in India: a stakeholder analysis

ABSTRACT Background: In India, 50–65% of the population face difficulties in accessing medicines. The Health Impact Fund (HIF) is a novel proposal whereby pharmaceutical companies would be paid based on the measured global health impact of their drugs. We conducted a key stakeholder analysis to explore access to medicines in India, acceptability of the HIF and potential barriers and facilitators at policy level. Objectives: To conduct a stakeholder analysis of the HIF in India: to determine key stakeholder views regarding access to medicines in India; to evaluate acceptability of the HIF; and to assess potential barriers and facilitators to the HIF as a policy. Methods: In New Delhi, we conducted semi-structured interviews. There was purposive recruitment of participants with snowball sampling. Transcribed data were analysed using stakeholder analysis frameworks and directed content analysis. Results: Participation rate was 29% (14/49). 14 semi-structured interviews were conducted among stakeholders in New Delhi. All participants highlighted access to medicines as a problem in India. There were mixed views about the HIF in terms of relevance and scaleability. Stakeholders felt it should focus on diseases with limited or no market and potentially incorporate direct investment in research. Conclusions: First, access to medicines is perceived to be a major problem in India by all stakeholders, but affordability is just one factor. Second, stakeholders despite considerable support for the idea of the HIF, there are major concerns about scaleability, generalisability and impact on access to medicines. Third, the HIF and other novel drug-related health policies can afford to be more radical, e.g. working outside the existing intellectual property rights regime, targeting generic as well as branded drugs, or extending to research and development. Further innovations in access to medicines must involve country-specific key stakeholders in order to increase the likelihood of their success.
New Design of T-Shaped Channel Drop Filter Based on Photonic Crystal Ring Resonator

In this paper, we propose a new design of T-shaped channel drop filter using photonic crystal ring resonator. The proposed structure is composed of two photonic crystal waveguides and a square photonic crystal ring resonator with circular inner rods configuration. Calculations of band structure and normalized transmission spectra are done by plane wave expansion (PWE) and finite difference time domain (FDTD) methods, respectively. According to the results, we note that 100% dropping efficiency with quality factor of 502 is achievable at wavelength λ=1.578487 μm. The effect of varying different parameters on the resonant wavelength and dropping efficiency of the filter is investigated. These parameters include refractive index, radius of inner and whole rods.
A Mathematical Model for Mechanically-Induced Deterioration of the Binder in Lithium-Ion Electrodes

This study is concerned with modeling detrimental deformations of the binder phase within lithium-ion batteries that occur during cell assembly and usage. A two-dimensional poroviscoelastic model for the mechanical behavior of porous electrodes is formulated and posed on a geometry corresponding to a thin rectangular electrode, with a regular square array of microscopic circular electrode particles, stuck to a rigid base formed by the current collector. Deformation is forced both by (i) electrolyte absorption driven binder swelling, and (ii) cyclic growth and shrinkage of electrode particles as the battery is charged and discharged. In order to deal with the complexity of the geometry, the governing equations are upscaled to obtain macroscopic effective-medium equations. A solution to these equations is obtained, in the asymptotic limit that the height of the rectangular electrode is much smaller than its width, which shows the macroscopic deformation is one-dimensional, and with growth confined to the ve.
Long-term effectiveness of rivastigmine patch or capsule for mild-to-severe Alzheimer’s disease: a meta-analysis

Aim: To evaluate the long-term effectiveness of rivastigmine patch or capsule on mild to severe Alzheimer’s disease (AD). Method: We performed a meta-analysis of 17 studies regarding the treatment effectiveness of rivastigmine patch or capsule on mild-to-severe AD. Results: Significant difference exists between treatment with rivastigmine patch or capsule and placebo groups (p-value < 0.001). In the Alzheimer’s Disease Assessment Scale-Cognitive subscale (ADAS-Cog) score evaluation, a negative weighted mean difference (WMD) was observed in overall and mild-moderate groups after rivastigmine treatment. And in ADAS-ADL score evaluation, a positive WMD was observed in overall groups after rivastigmine treatment. Moreover, WMD value is lower in patch administration subgroup compared to that of capsule administration subgroup. Conclusion: Rivastigmine treatment shows a positive result of improving the condition of patients with mild-to-severe AD. Patch administration shows a stronger effect on decreasing ADAS-Cog score compared to capsule administration.
A 97% high-efficiency 6μs fast-recovery-time buck-based step-up/down converter with embedded 1/2 and 3/2 charge-pumps for li-lon battery management

Lithium-ion batteries are generally used in mobile devices, but the voltage range of the battery varies from 2.7 to 4.2V. To provide a mid-3V-range output from the battery, a converter capable of step-up/down-conversion is necessary. For this purpose, non-inverting buck-boost topologies with multimode control [1-3] have been widely used. However, they have limited efficiency slightly higher than 90%, which comes from the fact that a main current path always encompasses two switches. To increase the efficiency in the buck mode where the converter operates for most of the usage time, a flying capacitor buck-boost (FCBB) was proposed in [4]. Despite its high power efficiency, it requires large-size LDMOS to endure a large voltage range up to 8V at switching node, resulting in cost inefficiency. Since all these topologies have a common controller that covers both buck and boost modes of operation, compensator design is challenging. Moreover, a non-minimum-phase system of boost operation makes it hard to achieve a fast loop response. In this paper, we propose a step-up/down DC-DC converter based on buck operation only over the whole input voltage range, which greatly simplifies the controller design and consequently gives fast response. Furthermore, it achieves high efficiency because of the reduced effective resistance on the main current path.
Structural parameters and polarization properties of TiN thin films prepared by reactive magnetron sputtering

We report the results of the investigation of morphological, structural, optical and plarimeteric properties of titanium nitride thin films deposited on silicon and glass substrates. The magnetron sputtered titanium nitride thin films were established to possess crystalline structure with the average grain size about D = 15 nm. The method of correlation matrix is was applied for the analysis of polarization properties of scattered light by the titanium nitride thin film. The obtained experimental result, can be explained by the presence of the effects of linear and circular dichroism in the material of the titanium nitride thin films under investigations.
A radiative transfer framework for rendering materials with anisotropic structure

The radiative transfer framework that underlies all current rendering of volumes is limited to scattering media whose properties are invariant to rotation. Many systems allow for "anisotropic scattering," in the sense that scattered intensity depends on the scattering angle, but the standard equation assumes that the structure of the medium is isotropic. This limitation impedes physics-based rendering of volume models of cloth, hair, skin, and other important volumetric or translucent materials that do have anisotropic structure. This paper presents an end-to-end formulation of physics-based volume rendering of anisotropic scattering structures, allowing these materials to become full participants in global illumination simulations. We begin with a generalized radiative transfer equation, derived from scattering by oriented non-spherical particles. Within this framework, we propose a new volume scattering model analogous to the well-known family of microfacet surface reflection models; we derive an anisotropic diffusion approximation, including the weak form required for finite element solution and a way to compute the diffusion matrix from the parameters of the scattering model; and we also derive a new anisotropic dipole BSSRDF for anisotropic translucent materials. We demonstrate results from Monte Carlo, finite element, and dipole simulations. All these contributions are readily implemented in existing rendering systems for volumes and translucent materials, and they all reduce to the standard practice in the isotropic case.
Full swing 20 GHz frequency divider with 1 V supply voltage in FD-SOI 28 nm technology

In this paper we present the design of a programmable frequency divider in 28 nm FD-SOI CMOS technology. It consists of the cascade of a divide-by-2 cell and divide-by-2/3 blocks. The final circuit is capable of dividing by even numbers between 128 and 254. The forward-body-bias property of the process and the differential-cascode voltage-switch-logic (DCVSL) family are used to achieve high operation speed. The proposed circuit achieves a maximum operating frequency of 20 GHz at 1 V supply voltage. And the area and the power consumption of the programmable divider are 1815 μm2 and 4.35 mW, respectively.
Biochemical studies on pesticides benomyl and propargite in rats

The effect of administration albino male rats a daily diet polluted with propargite and benomyl a concentration of 1.22,2.44 ,0.5 and 1.0 ppm respectively for 30 days, on the growth, biochemical parameters and its accumulation in organs. The tested pesticides significantly increased of body weight at all levels with different variation.Both pesticides significantly increased the levels of bilirubin uric acid, creatinine, Aspartate aminotransferease (AST) and Alanine aminotransferease (ALT) while significantly decrease the level of cholesterol and Alkaline phosphate (AP). The residues of propargite were detected in liver, kidney and spleen at low and high doses while benomyl residues were not detected in different organs except liver.
Thioflavin T as an amyloid dye: fibril quantification, optimal concentration and effect on aggregation

Formation of amyloid fibrils underlies a wide range of human disorders, including Alzheimer's and prion diseases. The amyloid fibrils can be readily detected thanks to thioflavin T (ThT), a small molecule that gives strong fluorescence upon binding to amyloids. Using the amyloid fibrils of Aβ40 and Aβ42 involved in Alzheimer's disease, and of yeast prion protein Ure2, here we study three aspects of ThT binding to amyloids: quantification of amyloid fibrils using ThT, the optimal ThT concentration for monitoring amyloid formation and the effect of ThT on aggregation kinetics. We show that ThT fluorescence correlates linearly with amyloid concentration over ThT concentrations ranging from 0.2 to 500 µM. At a given amyloid concentration, the plot of ThT fluorescence versus ThT concentration exhibits a bell-shaped curve. The maximal fluorescence signal depends mostly on the total ThT concentration, rather than amyloid to ThT ratio. For the three proteins investigated, the maximal fluorescence is observed at ThT concentrations of 20–50 µM. Aggregation kinetics experiments in the presence of different ThT concentrations show that ThT has little effect on aggregation at concentrations of 20 µM or lower. ThT at concentrations of 50 µM or more could affect the shape of the aggregation curves, but this effect is protein-dependent and not universal.
Social Basis of Vocal Interactions in Western Lowland Gorillas (Gorilla g. gorilla)

Authors have raised the possibility of identifying primitive forms of conversational rules in monkeys: temporally ruled vocal interactions, call overlap avoidance, and socially based calling partner preferences. The question as to how these abilities have evolved in the primate lineage remains open to debate, particularly because studies based on apes are scarce and controversial. We studied a captive group of western lowland gorillas and tested the influence of caller characteristics and the type of bond between calling partners on vocal behavior based on the following: age, dominance, spatial proximity, sociopositive contact, and gaze. Four calling patterns that are call type dependent were identified: vocal interaction with and (more frequently) without call overlap, isolated calling, and repeated calling. Adult calls and grunts (contact calls) were predominant during vocal interactions, and the “response” delay was most often around half a second. The frequency of grunt dyadic exchanges was found to be linked to spatial proximity, gaze exchanges, and age proximity between calling partners. The dominance rank of callers determined the rate of contribution to these exchanges. These results show that some apes use rule-governed call exchanges and that these socially guided vocal interactions are more widespread than previously believed.
Palmprint identification and verification with minimal number of features

In this paper, palmprint verification and identification with minimum number of features is proposed. The wide principal line extractors (WPLEs) on the region of interest (ROI) are applied to generate wide principal line images (WPLIs). The WPLI is segmented into 2 × 2, 4 × 4, 8 × 8 and 16 × 16 and the feature value is extracted directly from each segment. Experiments are conducted by using the extracted features. The results show that the equal error rate (EER), decidability index (DI) and correct recognition rate (CRR) of the proposed approach is better than existing methods for PolyUPalmprint Database.
Tissue Destruction in Bullous Pemphigoid Can Be Complement Independent and May Be Mitigated by C5aR2

Bullous pemphigoid (BP), the most frequent autoimmune bullous disorder, is a paradigmatic autoantibody-mediated disease associated with autoantibodies against BP180 (type XVII collagen, Col17). Several animal models have been developed that reflect important clinical and immunological features of human BP. Complement activation has been described as a prerequisite for blister formation, however, the recent finding that skin lesions can be induced by anti-Col17 F(ab′)2 fragments indicates complement-independent mechanisms to contribute to blister formation in BP. Here, C5−/− mice injected with anti-Col17 IgG showed a reduction of skin lesions by about 50% associated with significantly less skin-infiltrating neutrophils compared to wild-type mice. Reduction of skin lesions and neutrophil infiltration was seen independently of the employed anti-Col17 IgG dose. Further, C5ar1−/− mice were protected from disease development, whereas the extent of skin lesions was increased in C5ar2−/− animals. Pharmacological inhibition of C5a receptor 1 (C5aR1) by PMX53 led to reduced disease activity when applied in a prophylactic setting. In contrast, PMX-53 treatment had no effect when first skin lesions had already developed. While C5aR1 was critically involved in neutrophil migration in vitro, its role for Col17-anti-Col17 IgG immune complex-mediated release of reactive oxygen species from neutrophils was less pronounced. Our data demonstrate that complement-dependent and -independent mechanisms coexist in anti-Col17-autoantibody-mediated tissue destruction. C5aR1 and C5aR2 seem to play opposing roles in this process with C5aR1 exerting its primary effect in recruiting inflammatory cells to the skin during the early phase of the disease. Further studies are required to fully understand the role of C5aR2 in autoantibody-mediated skin inflammation.
Carotenoid supplementation and retinoic acid in immunoglobulin A regulation of the gut microbiota dysbiosis

Dysbiosis, a broad spectrum of imbalance of the gut microbiota, may progress to microbiota dysfunction. Dysbiosis is linked to some human diseases, such as inflammation-related disorders and metabolic syndromes. However, the underlying mechanisms of the pathogenesis of dysbiosis remain elusive. Recent findings suggest that the microbiome and gut immune responses, like immunoglobulin A production, play critical roles in the gut homeostasis and function, and the progression of dysbiosis. In the past two decades, much progress has been made in better understanding of production of immunoglobulin A and its association with commensal microbiota. The present minireview summarizes the recent findings in the gut microbiota dysbiosis and dysfunction of immunoglobulin A induced by the imbalance of pathogenic bacteria and commensal microbiota. We also propose the potentials of dietary carotenoids, such as β-carotene and astaxanthin, in the improvement of the gut immune system maturation and immunoglobulin A production, and the consequent promotion of the gut health. Impact statement The concept of carotenoid metabolism in the gut health has not been well established in the literature. Here, we review and discuss the roles of retinoic acid and carotenoids, including pro-vitamin A carotenoids and xanthophylls in the maturation of the gut immune system and IgA production. This is the first review article about the carotenoid supplements and the metabolites in the regulation of the gut microbiome. We hope this review would provide a new direction for the management of the gut microbiota dysbiosis by application of bioactive carotenoids and the metabolites.
A 3-D-Printed Integrated PCB-Based Electrochemical Sensor System

In this paper, we report a fully 3-D-printed, flexible, amperometric lactate sensor system with a 3-D-printed, integrated electrochemical circuit platform, a so-called potentiostat, realized via a novel 3-D printing method. First, a lactate sensor was designed in a three-electrode configuration with a layer of deposited enzyme on the conductive silver electrodes. The thin silver electrodes were printed on a flexible substrate. Second, a highly integrated potentiostat circuit prototype was designed and printed on a rigid epoxy board. Both sensor electrodes and printed circuit board (PCB) were fabricated using a novel direct ink writing (DIW) technology with highly viscous silver nanoparticle ink. The fully 3-D-printed potentiostat system along with the sensor demonstrated reliable lactate detection for concentrations in the range of 0–20 mM. The purely additive manufacturing strategy of DIW demonstrated the capability of 3-D printing double-sided circuits with high-density chip-on-board designs as well as the ability to significantly reduce the lead time of PCB prototyping. The 3-D-printed electrochemical sensor system is suitable for wearable devices for continuous monitoring of human metabolisms.
GhJAZ2 attenuates cotton resistance to biotic stresses via the inhibition of the transcriptional activity of GhbHLH171.

Plants have evolved effective mechanisms to protect themselves against multiple stresses, and employ jasmonates (JAs) as vital defence signals to defend against pathogen infection. The accumulation of JA, induced by signals from biotic and abiotic stresses, results in the degradation of Jasmonate-ZIM-domain (JAZ) proteins, followed by the de-repression of JAZ-repressed transcription factors (such as MYC2) to activate defence responses and developmental processes. Here, we characterized a JAZ family protein, GhJAZ2, from cotton (Gossypium hirsutum) which was induced by methyl jasmonate (MeJA) and inoculation of Verticillium dahliae. The overexpression of GhJAZ2 in cotton impairs the sensitivity to JA, decreases the expression level of JA-response genes (GhPDF1.2 and GhVSP) and enhances the susceptibility to V. dahliae and insect herbivory. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that GhJAZ2 may be involved in the regulation of cotton disease resistance by interaction with further disease-response proteins, such as pathogenesis-related protein GhPR10, dirigent-like protein GhD2, nucleotide-binding site leucine-rich repeat (NBS-LRR) disease-resistant protein GhR1 and a basic helix-loop-helix transcription factor GhbHLH171. Unlike MYC2, overexpression of GhbHLH171 in cotton activates the JA synthesis and signalling pathway, and improves plant tolerance to the fungus V. dahliae. Molecular and genetic evidence shows that GhJAZ2 can interact with GhbHLH171 and inhibit its transcriptional activity and, as a result, can restrain the JA-mediated defence response. This study provides new insights into the molecular mechanisms of GhJAZ2 in the regulation of the cotton defence response.