Miracle-dz/UniFAR-FTUs huggingface dataset:
Massilia putida sp. nov., a dimethyl disulfide-producing bacterium isolated from wolfram mine tailing.

A heavy metal-resistant and dimethyl disulfide-producing bacterial strain, designated 6NM-7T, was isolated from wolfram mine tailing, Dayu County, Jiangxi Province, PR China. Strain 6NM-7T was aerobic, Gram-stain-negative and motile by means of a single polar flagellum. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain 6NM-7T was affiliated with the genus Massilia and was closely related to Massilia norwichensis LMG 28164T (98.8 % 16S rRNA gene sequence similarity), Massilia kyonggiensis KACC 17471T (98.4 %), Massilia niastensis KACC 12599T (97.8 %), Massilia tieshanensis KACC 14940T (97.3 %), Massilia haematophila KACC 13771T (97.2 %), Massilia namucuonensis CGMCC 1.11014T (97.1 %) and Massilia aerilata KACC 12505T (97.1 %). The DNA-DNA relatedness values between strain 6NM-7T and its closely related type strains were all below 70 %. The major respiratory quinone was unbiquinone 8 (Q-8) and the major cellular fatty acids consisted of C16 : 0 (33.2 %), summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH; 21.8 %), C17 : 0 cyclo (20.8 %), C18 : 1ω7c (7.4 %) and C10 : 0 3-OH (5.8 %). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of strain 6NM-7T was 66.8 ± 0.6 mol%. On the basis of the results of this polyphasic taxonomic study, strain 6NM-7T should be assigned to a novel species of the genus Massilia, for which the name Massilia putida sp. nov. is proposed. The type strain is 6NM-7T ( = DSM 27523T = KCTC 42761T).
Economic Crises and Unemployment Persistence: Analysis of Job Losses During the Finnish Recession of the 1990s

This study estimates the effect of job loss for Finnish workers who were displaced during a severe recession in the early 1990s. The setting provides a sample of workers of which over 90% experienced unemployment after losing a job due to plant closure. We use linked employer&#8211;employee data to follow these workers up to 16 years after the job loss and to construct a closely matched comparison group of non&#8208;displaced workers. We estimate a more than 58% initial drop in employment and earnings after the job loss. At the end of follow&#8208;up, women's employment and earnings recover fully while men's employment remains 1&#8211;5% lower relative to the comparison groups, and men suffer a 5&#8211;8% earnings loss. We also find large effects on long&#8208;term unemployment that diminish slowly, and long&#8208;lasting effects on poverty risk.
Sentence verification and delusions: A content-specific deficit

Background. A sentence verification task was developed to investigate semantic memory in schizophrenia. Methods. The test consisted of three types of sentence (true, unlikely and nonsense) and seven different types of content (neutral, persecutory, grandiose, political, religious, relationships and somatic) representing common delusional themes present in schizophrenic patients. Sixty-three schizophrenic patients and 66 matched control subjects were asked to make true/false judgements to 143 sentences. Results. Overall accuracy was similar across the two groups; sentences with some emotional themes and sentences of the unlikely type produced the most violations. Significant differences between the two subject groups were found specifically on nonsense sentences with persecutory and religious themes. Patients made significantly more incorrect responses (acceptance) to nonsense sentences that had an emotional content congruent with their delusional beliefs, past or present, and also on unlikely sentences (incorrect rejections) whose content was not congruent with their delusions. Further analysis of response bias in the patients showed, overall, that there were more incorrect rejections (a reflection of the large number of unlikely sentence errors) and more incorrect responses to sentences congruent with patients delusions. Furthermore, analysis of those patients currently experiencing delusions revealed more incorrect responses to sentences congruent with their delusional ideas compared with patients not currently deluded. Conclusions. These findings are indicative of cognitive bias in schizophrenia towards certain emotional themes that may underlie illogical semantic connections and delusions.
The Relationship Between Internal Auditing and External Audit Fees: Evidence from Kuwait

Although audit pricing has been one of the most studied topics in theaudit literature for more than three decades now, to date, very little research hasbeen conducted on this important issue in the Middle East Region. One importantquestion in this line of audit research has been related to whether audit fees areinfluenced by the contribution of client’s internal auditing (IA) to the externalaudit work. Much of existing research investigating this issue has been conductedin well-developed English-speaking countries, with almost no empirical evidenceprovided about this issue within the context of other parts of the world. Thepurpose of the current study is to examine this issue using data from the Kuwaitiaudit market. In particular, the current study uses a sample of audit engagementsperformed in the Kuwaiti market, to examine whether external audit fees areinfluenced by the contribution of the client’s internal audit function. The resultsshow that IA contribution in the external audit work is negatively related to theamount of external audit fees.
Human neural responses elicited to observing the actions of others

Monkey electrophysiological and human neuroimaging studies indicate the existence of specialized neural systems for the perception and execution of actions. To date, the dynamics of these neural systems in humans have not been well studied. Here, we investigated the spatial and temporal behavior of human neural responses elicited to viewing motion of the face, hand, and body. Scalp event-related potentials (ERPs) were recorded in 20 participants viewing videotaped mouth (opening, closing), hand (closing, opening), and body stepping (forward, backward) movements. ERP peak differences within the movements of each body part were compared using topographical maps of voltage, voltage difference, and Student's t-test at ERP peak latencies. Predominantly temporoparietal negative ERPs occurred to motion of all body parts within 200 ms postmovement onset. Hand closure elicited a significantly greater negativity than opening, particularly in the left hemisphere. Vertex positive ERPs within 300 ms postmovement onset were elicited to hand and body motion. A significantly greater positivity occurred for the body stepping forward relative to stepping backward. The ERP topography was consistent with observed activation foci in human neuroimaging studies. Our data indicate that the neural activity of a system dedicated to the perception of high-level motion stimuli can rapidly differentiate between movements across and within body parts.
Utility of a human FcRn transgenic mouse model in drug discovery for early assessment and prediction of human pharmacokinetics of monoclonal antibodies

ABSTRACT Therapeutic antibodies continue to develop as an emerging drug class, with a need for preclinical tools to better predict in vivo characteristics. Transgenic mice expressing human neonatal Fc receptor (hFcRn) have potential as a preclinical pharmacokinetic (PK) model to project human PK of monoclonal antibodies (mAbs). Using a panel of 27 mAbs with a broad PK range, we sought to characterize and establish utility of this preclinical animal model and provide guidance for its application in drug development of mAbs. This set of mAbs was administered to both hemizygous and homozygous hFcRn transgenic mice (Tg32) at a single intravenous dose, and PK parameters were derived. Higher hFcRn protein tissue expression was confirmed by liquid chromatography-high resolution tandem mass spectrometry in Tg32 homozygous versus hemizygous mice. Clearance (CL) was calculated using non-compartmental analysis and correlations were assessed to historical data in wild-type mouse, non-human primate (NHP), and human. Results show that mAb CL in hFcRn Tg32 homozygous mouse correlate with human (r2 = 0.83, r = 0.91, p < 0.01) better than NHP (r2 = 0.67, r = 0.82, p < 0.01) for this dataset. Applying simple allometric scaling using an empirically derived best-fit exponent of 0.93 enabled the prediction of human CL from the Tg32 homozygous mouse within 2-fold error for 100% of mAbs tested. Implementing the Tg32 homozygous mouse model in discovery and preclinical drug development to predict human CL may result in an overall decreased usage of monkeys for PK studies, enhancement of the early selection of lead molecules, and ultimately a decrease in the time for a drug candidate to reach the clinic.
Gene expression of connective tissue growth factor in adult mouse

The connective tissue growth factor (CTGF) is a well-known fibroblast mitogen and angiogenic factor that plays an important role in bone formation during embryogenesis. In the adult, CTGF is involved in wound healing as well as fibrotic and vascular disease. However, little is known about its physiological functions under non-pathological conditions in the adult organism. Here, we describe the cellular site of the CTGF mRNA expression in adult male and female mice as revealed by in situ hybridization histochemistry. Strong and persistent CTGF gene expression was particularly prominent in the mesenchyme of the cardiovascular system (aorta, auricular tissue, renal glomeruli), the mesenchyme surrounding the ovarian follicles or the testicular tubes in the gonadal tissue, and the subcapsular mesenchyme bordering densely innervated parts of whisker hair vibrissae. CTGF hybridization signals were not observed in the mesenchyme of many other organs including gut, muscle, liver or most parts of the lymphatic tissue. Strong expression was also present in the primary (early) ovarian follicles, the epithelium of the deep uterine glands and on myenteric ganglia neurons. These data suggest a selective and continuous mesenchymal function in the gonads and those tissues attracting very strong vascular supply or peripheral innervation. CTGF may also be involved in the cyclical proliferation of the uterine gland epithelium and in the early stages of follicular maturation, as well as in the neuropeptide regulation in the gut, cardiovascular and renal systems.
The Rise in Corporate Saving and Cash Holding in Advanced Economies: Aggregate and Firm Level Trends

Using cross-country national accounts and firm-level data, we document a broad-based trend in rising gross saving and net lending of non-financial corporates across major industrialized countries over the last two decades, though most pronounced in countries with persistent current account surpluses. We find that this trend holds consistently across major industries, and is concentrated among large firms, driven by rising profitability, lower financing costs, and reduced tax rates. At the same time, higher gross corporate saving have not supported a commensurate increase in fixed capital investment, but instead led to a build-up of liquid financial assets (cash). The determinants of corporate cash holding and saving are also broad-based across countries, with the growth in assets of large firms, R&D intensity, and lower effective tax rates accounting for most of the increase over the last 15 years.
Reproducibility of endurance performance on a treadmill using a preloaded time trial.

PURPOSE The purpose of this study was to establish a highly reproducible test to measure endurance performance in runners. METHODS We evaluated the reproducibility of endurance performance during a 10-km time trial performed on a treadmill after a 90-min preload run at 65% of maximal oxygen uptake VO2max). After screening and a practice test, eight endurance runners (4 men, 4 women, 33.4 +/- 10.1 yr, VO2max = 60.3 +/- 6.3 mL x kg(-1) x min(-1) in men and 51.8 +/- 2.2 mL x kg(-1) x min(-1) in women, mean +/- SD) completed two preloaded time trial tests spaced 3-4 wk apart in men and one menstrual cycle apart in women. A high-carbohydrate diet (15% protein, 10% fat, 75% carbohydrate) was provided the day before both tests. RESULTS Runners completed time trial 1 and time trial 2 in 45:41 +/- 4:45 and 45:24 +/- 5:03 min:s, respectively (43:29 +/- 5:02 and 43:12 +/- 5:14 min:s for men and 47:53 +/- 3:47 and 47:35 +/- 4:23 min:s for women, trials 1 and 2, respectively). The within-subject coefficient of variation for 10-km time was 1.00% +/- 0.25% (point estimate +/- estimated standard error) (0.54% +/- 0.19% for men and 1.26% +/- 0.45% for women). CONCLUSION These results suggest that performance measured as time to complete a 10-km time trial on a treadmill after a 90-min preload is extremely reliable and may be useful for future research assessing the effect of diet, ergogenic substances, or training methods on endurance running performance.
Artemisia iwayomogi Inhibits Immediate-Type Allergic Reaction and Inflammatory Cytokine Secretion

The immediate-type allergic reaction is involved in many allergic diseases such as asthma and allergic rhinitis. The discovery of drugs for the treatment of immediate-type allergic diseases is a very important subject in human health. In this study, we investigated the effect of Artemisia iwayomogi (AIAE) on mast cell-mediated allergic reaction and inflammatory cytokine secretion. AIAE inhibited compound 48/80-induced systemic reactions in mice. AIAE decreased the passive cutaneous anaphylaxis (PCA) reaction activated by antidinitrophenyl (anti-DNP) IgE antibody. AIAE dose-dependently reduced histamine release from rat peritoneal mast cells activated by compound 48/80 or anti-DNP IgE. Furthermore, AIAE attenuated the phorbol 12‐myristate 13-acetate plus calcium ionophore A23187-stimulated tumor necrosis factor-α and interleukin-6 secretion in human mast cells. These results provide evidence that AIAE may be beneficial in the treatment of allergic diseases.
High Incidence of Xenogenic Bone Marrow Engraftment in Pig‐to‐Baboon Intra‐Bone Bone Marrow Transplantation

Previous attempts of α‐1,3‐galactocyltransferase knockout (GalTKO) pig bone marrow (BM) transplantation (Tx) into baboons have demonstrated a loss of macro‐chimerism within 24 h in most cases. In order to achieve improved engraftment with persistence of peripheral chimerism, we have developed a new strategy of intra‐bone BM (IBBM) Tx. Six baboons received GalTKO BM cells, with one‐half of the cells transplanted into the bilateral tibiae directly and the remaining cells injected intravenously (IBBM/BM‐Tx) with a conditioning immunosuppressive regimen. In order to assess immune responses induced by the combined IBBM/BM‐Tx, three recipients received donor SLA‐matched GalTKO kidneys in the peri‐operative period of IBBM/BM‐Tx (Group 1), and the others received kidneys 2 months after IBBM/BM‐Tx (Group 2). Peripheral macro‐chimerism was continuously detectable for up to 13 days (mean 7.7 days; range 3–13) post‐IBBM/BM‐Tx and in three animals, macro‐chimerism reappeared at days 10, 14 and 21. Pig CFUs, indicating porcine progenitor cell engraftment, were detected in the host BM in four of six recipients on days 14, 15, 19 and 28. In addition, anti‐pig unresponsiveness was observed by in vitro assays. GalTKO/pCMV‐kidneys survived for extended periods (47 and 60 days). This strategy may provide a potent adjunct for inducing xenogeneic tolerance through BM‐Tx.
Exploring the Problems and Challenges of Micro, Small and Medium Enterprises in Northern Province

The purpose of this study is to identify the problems and challenges of SMEs in the postwar situation of Northern Province. Researcher conducted qualitative exploratory research; explain the phenomenon of problematic situation of SMEs in Northern Province. By applying purposive sampling technique, with primary data collection method of focus group discussion and interviews were conducted with the owners of SMEs and officers related with the SMEs in the Northern Province. Finding of the study explains, first, marketing problems which includes product problems, pricing problems, promotion problem, distribution based problems, green problem and network problems, second, internal problem which includes financial problems and employee problems third, characteristics of entrepreneurs as problems, at last external problems, namely political and legal, economic and socio economic problems. The important implication is that this study indicates clearly the problems and challenges of SMEs in the post war market. It will help the owners of SMEs the means to solve these problems, and govern officers to understand the means to support the SMEs.
Differential expression of two related amino acid transporters with differing substrate specificity in Arabidopsis thaliana.

A general amino acid permease cDNA (AAP2) was isolated from Arabidopsis by complementation of a yeast mutant defective in citrulline uptake. Direct transport measurements in yeast show that the protein mediates uptake of L-[14C]-citrulline and L-[14C]-proline. Detailed analyses of the substrate specificity by competition studies demonstrate that all proteogenic amino acids are recognized by the carrier, including those that represent the major transport forms of reduced nitrogen in many species, i.e. glutamine, glutamate and asparagine. Thus, AAP2 is less selective as compared with AAP1 and transports basic amino acids such as histidine as shown by expression in a histidine transport-deficient yeast strain. The predicted polypeptide of 53 kDa is highly hydrophobic with 12 putative membrane-spanning regions and shows significant homologies to the Arabidopsis broad specificity permease AAP1, and a limited homology to bacterial branched chain amino acid transporters, but not to any other known proteins. Alterations in the charged residues as compared with AAP1 in four regions might be involved in the difference in selectivity towards basic amino acids. Both genes are highly expressed in developing pods indicating a role in supplying the developing seeds with reduced nitrogen. AAP2 is selectively expressed in the stem and might therefore play a role in xylem-to-phloem transfer of amino acids during seed filling. Furthermore in situ hybridization shows that both genes are expressed in the vascular system of cotyledons in developing seedlings.
Interleukin-1 and Interleukin-6 Activities are Increased in the Cerebrospinal Fluid of Patients with CNS Lupus Erythematosus and Correlate with Local Late T-Cell Activation Markers

We examined cerebrospinal fluid (CSF) samples from 12 patients with SLE and active central nervous system (CNS) involvement for their levels of the following cytokines: interleukin- 1 (IL-1) by means of two different assays-the IL-1 responsive murine cell line LBRM 33-1a5 and an ELISA for IL-1 alpha; IL-2 by means of the CTLL cell line responsive to it; and interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF) both determined by a specific ELISA. We found that SLE CSF had significantly higher levels of IL-1 and IL-6 than did those obtained at surgery from eight controls without inflammatory neurologic disease. IL-2 and TNF were not detectable in any of the CSF samples. We also studied the status of activation in CSF T cells using monoclonal antibodies against early (anti-IL-2R (CD25) and antitransferrin (CD71)), late (anti-T10) and very late (anti-VLA-1) activation antigens, and found increased percentages of T10-bearing (18 ± 2 vs 3 ± 0.7%) and VLA-1-bearing T cells (12 ± 2 vs 0.7 ± 0.2%) in SLE patients as compared to controls (both P < 0.01). Levels of IL-1 and IL-6 correlated with T10 and those of IL-1 correlated also with VLA-1. Markers of early T-cell activation did not differ in SLE and control CSF. Because of these findings we analysed the effect of recombinant IL,-I, IL-6 or normal CSF on normal T cells and found that they did not induce the expression of activation markers. However, incubation in SLE CSF caused CD25, T10 and VLA-1 activation markers to become significantly expressed on cultured normal T cells. This expression of T10 and VLA-1 was partially inhibited by pre-incubation in anti-human IL-1 alpha polyvalent antibody. Our findings suggest increased in situ production of IL- and IL-6 and perhaps other factors in CNS lupus that might condition T-cell activation in the CNS compartment. These findings could have pathogenetic significance.
3D simulation of filler concentration in semiconductor processing

Due to the tighter and tighter requirement for modern electronic product, the smaller dimensional specification is expected to fit under the evolution of continuous process. On the other hand, under the pressure of time-to-market, how to provide qualified modern electronic products is more and more challenge for the associated package process. Specifically, one of the important defects is non-uniform property result from un-even filler particle distribution, temperature profile, and local gelation change during the package process. To catch the phenomena, CAE simulation is commonly applied. However, most CAE simulations assume the material property is homogeneous. This assumption will be far from the situation in reality, for example regarding to shear-induced migration and particle settling. In this study, a three-dimensional simulation model of noncolloidal filler suspension is proposed to predict the filler concentration in microchips. Firstly, the proposed model is validated using two-dimensional channel and axisymmetrical circular pipe geometry model. Results showed that the trend of filler distribution is in a good agreement. Furthermore, transfer molding process is utilized to study the inhomogeneity of fillers due to settling and shear migration. Particularly, there are three different experimental factors discussed to see the effect that induce distinct filler concentration distributions after molding. The results will provide a comprehensive understanding the driving force for filler concentration. By using the integrated analysis, filler concentration during encapsulation can be easily detected to efficiently reduce manufacturing cost and design cycle time.
Role of patriotism in explaining event attendance intention and media consumption intention: the case of Rio 2016

ABSTRACT This study investigates the role of patriotism in explaining event attendance intention and media consumption intention for mega-sporting events. With Rio 2016 as the context, this study utilizes the model of goal-directed behavior as an underlying mechanism to explain the role of patriotism and two different intentions in the context of mega-sporting events. Results confirm that patriotism does not influence one’s desire and intention to attend the event physically, but it directly influences one’s intention to watch the game through media platforms. The International Olympic Committee and media companies need to strengthen patriotism to increase viewership during the event.