Miracle-dz/UniFAR-FTUs huggingface dataset:
Macroinvertebrate community response to eutrophication in an oligotrophic wetland: An in situ mesocosm experiment

Eutrophication from anthropogenic nutrient enrichment is a primary threat to the oligotrophic freshwater marshes of southern Florida. Macrophyte and periphyton response to increased phosphorus (P) has been well documented in both correlative and experimental studies, but the response of consumer communities remains poorly understood, especially in southern marl prairies. We conducted a P-loading experiment in in situ mesocosms in Taylor Slough, Everglades National Park, and examined the response of macroinvertebrate communities. Mesocosms at two sites were loaded weekly with P at four levels: control (0 g P/m2/yr), low (0.2 g P/m2/yr), intermediate (0.8 g P/m2/yr), and high (3.2 g P/m2/ yr). After ∼2 yrs of P-loading, macroinvertebrates were sampled using periphyton mat and benthic floc cores. Densities of macroinvertebrate taxa (no./g AFDM) were two to 16 times higher in periphyton mats than benthic floc. Periphyton biomass decreased with enrichment at one site, and periphyton was absent from many intermediate and all high P treatments at both sites. Total macroinvertebrate density in periphyton mats increased with intermediate P loads, driven primarily by chironomids and nematodes. Conversely, total macroinvertebrate density in benthic floc decreased with enrichment, driven primarily by loss of chironomids and ceratopogonids (Dasyhelea). This study suggests that macroinvertebrate density increases with enrichment until periphyton mats are lost, after which it decreases, and mat infauna fail to move into benthic substrates in response to mat loss. These results were noted at nutrient levels too low to yield anoxia, and we believe that the decrease of macroinvertebrate density resulted from a loss of habitat. This work illustrates the importance of periphyton mats as habitat for macroinvertebrates in the Everglades. This study also indicates that in this system, macroinvertebrate sampling should be designed to target periphyton mats or conducted with special attention to inclusion of substrates relative to their coverage.
A novel method of resistance for influenza against a channel‐blocking antiviral drug

Effective antivirals are few and far between, and as such, the appearance of resistance toward such treatments is an obvious medical concern. In this article, we analyze the mechanism by which influenza attains resistance toward amantadine, a blocker of the viral M2 H+ channel. Binding analyses of amantadine to M2 peptides from different viral strains showed that the virus has developed two alternate routes to avoid blockage of its channel: (1) a conventional route, in which the channel no longer binds the blocker and, hence, the blocker cannot exert its inhibitory function; and (2) a novel mechanism, in which binding of the blocker is retained, yet the function of the protein is unaffected. Pore diameter profiles revealed the molecular mechanism by which the virus may attain this novel type of resistance: an increase in the size of the channel. Thus, despite the drug binding the channel, it may not be able to block the pore, since the channel diameter has increased. Our findings may have broad ramifications in the design of new antivirals, and of novel blockers against malfunctioning human channels implicated in disease. Proteins 2004. © 2004 Wiley‐Liss, Inc.
The role of the state in shaping zero hours work in an atypical liberal market economy

Zero hours work typifies work where there are no guaranteed hours offered by the employer. This article examines the relationship between the state and the emergence of zero hours work in an atypical liberal market economy, Ireland. Based on interviews with informed stakeholders with a focus on four sectors – retail, health, education and accommodation/food – the article concludes that the actions of the state have created a weak regulatory environment that has facilitated the emergence of zero hours work. The findings are discussed with a theoretical frame using the concepts of accumulation and legitimation.
Small-size printed antenna with shaped circuit board for slim LTE/WWAN smartphone application

An on-board printed WWAN/LTE antenna of simple structure disposed in a small clearance of 8 × 36 mm2 in the ground plane of shaped circuit board in a slim handset is presented. The shaped circuit board has a large rectangular notch such that the battery of the handset can be embedded therein to decrease the thickness of the handset, and lead to much enhanced bandwidth of the antenna disposed thereon. This is mainly because stronger surface currents on the ground plane of the shaped circuit board can be excited, which greatly helps improve the operating bandwidth of the antenna disposed thereon. The proposed design makes a simple, small-size printed PIFA capable of providing two wide operating bands to cover the GSM850/900 and GSM1800/1900/UMTS/LTE2300/2500 bands.
Identification of inhibitory scFv antibodies targeting fibroblast activation protein utilizing phage display functional screens

Fibroblast activation protein (FAP) is a serine protease selectively expressed on tumor stromal fibroblasts in epithelial carcinomas and is important in cancer growth, adhesion, and metastases. As FAP enzymatic activity is a potent therapeutic target, we aimed to identify inhibitory antibodies. Using a competitive inhibition strategy, we used phage display techniques to identify 53 single‐chain variable fragments (scFvs) after three rounds of panning against FAP. These scFvs were expressed and characterized for binding to FAP by surface plasmon resonance and flow cytometry. Functional assessment of these antibodies yielded an inhibitory scFv antibody, named E3, which could attenuate 35% of FAP cleavage of the fluorescent substrate Ala‐Pro‐7‐amido‐4‐trifluoromethylcoumarin compared with nonfunctional scFv control. Furthermore, a mutant E3 scFv was identified by yeast affinity maturation. It had higher affinity (4‐fold) and enhanced inhibitory effect on FAP enzyme activity (3‐fold) than E3. The application of both inhibitory anti‐FAP scFvs significantly affected the formation of 3‐dimensional FAP‐positive cell matrix, as demonstrated by reducing the fibronectin fiber orientation from 41.18% (negative antibody control) to 34.06% (E3) and 36.15% (mutant E3), respectively. Thus, we have identified and affinity‐maturated the first scFv antibody capable of inhibiting FAP function. This scFv antibody has the potential to disrupt the role of FAP in tumor invasion and metastasis. —Zhang, J., Valianou, M. , Simmons, H. , Robinson, M. K. , Lee, H. ‐O. , Mullins, S. R. , Marasco, W. A. , Adams, G. P., Weiner, L. M. , Cheng, J. D. Identification of inhibitory ScFv antibodies targeting fibroblast activation protein utilizing phage display functional screens. FASEB J. 27, 581–589 (2013). www.fasebj. org
Optimized dynamic response of parallel operation of two single phase inverters based on evolutionary theory

In this work it is proposed an optimized dynamic response of parallel operation of two single-phase inverters with no control communication. The optimization aims the tuning of the slopes of P-ω and Q-V curves so that the system is stable, damped and minimum settling time. The slopes are tuned using an algorithm based on evolutionary theory. Simulation and experimental results are presented to prove the feasibility of the proposed approach.
Control of Spoken Vowel Acoustics and the Influence of Phonetic Context in Human Speech Sensorimotor Cortex

Speech production requires the precise control of vocal tract movements to generate individual speech sounds (phonemes) which, in turn, are rapidly organized into complex sequences. Multiple productions of the same phoneme can exhibit substantial variability, some of which is inherent to control of the vocal tract and its biomechanics, and some of which reflects the contextual effects of surrounding phonemes (“coarticulation”). The role of the CNS in these aspects of speech motor control is not well understood. To address these issues, we recorded multielectrode cortical activity directly from human ventral sensory-motor cortex (vSMC) during the production of consonant-vowel syllables. We analyzed the relationship between the acoustic parameters of vowels (pitch and formants) and cortical activity on a single-trial level. We found that vSMC activity robustly predicted acoustic parameters across vowel categories (up to 80% of variance), as well as different renditions of the same vowel (up to 25% of variance). Furthermore, we observed significant contextual effects on vSMC representations of produced phonemes that suggest active control of coarticulation: vSMC representations for vowels were biased toward the representations of the preceding consonant, and conversely, representations for consonants were biased toward upcoming vowels. These results reveal that vSMC activity for phonemes are not invariant and provide insight into the cortical mechanisms of coarticulation.
Corneal density with the Pentacam after photorefractive keratectomy.

PURPOSE To measure corneal density and evaluate corneal haze in unoperated eyes and in eyes after photorefractive keratectomy (PRK). METHODS Forty-nine patients (91 eyes) who underwent PRK and 48 control patients (76 unoperated eyes) were measured with the densitometry program of the Pentacam Scheimpflug imaging system (Oculus Optikgeräte GmbH) (0=no clouding, 100=tissue completely opaque). Eyes were categorized as myopic or hyperopic (myopia group and hyperopia group), with subgroups defined as unoperated (unoperated myopia group and unoperated hyperopia group), postoperative clear corneas (clear cornea myopia group and clear cornea hyperopia group), and postoperative with haze (myopia haze group and hyperopia haze group). The ANOVA option of the Statistica 8.1 program package (StatSoft Inc) was used to compare subgroups. RESULTS Maximum density of postoperative corneas with haze (myopia haze group, 46.2±16.2; hyperopia haze group, 50.2±25.8) was higher than in postoperative clear corneas (clear cornea myopia group, 29.9±8.7; clear cornea hyperopia group, 22.9±3.1), but maximum corneal density in the latter subgroups showed no difference compared with unoperated controls (unoperated myopia group, 25.1±2.4; unoperated hyperopia group, 22.8±3.2). Differences among subgroups were not found outside the 6-mm diameter ablation zone. In hazy myopic corneas, there was a strong decreasing density trend from the apex to the 3-mm radius (P<.001, R=-0.5), which continued towards the periphery at 4.5 mm (P=.019, R=-0.21). Hyperopic mild hazy corneas showed a density increase from the apex to 3 mm (P<.001, R=0.4), followed by a decrease towards the periphery (P<.01, R=-0.4). CONCLUSIONS The densitometry program of the Pentacam can provide a useful objective measure of postoperative and other mild corneal haze.
Cost-effectiveness of a primary care treatment program for depression in low-income women in Santiago, Chile.

OBJECTIVE The authors compared the incremental cost-effectiveness of a stepped-care, multicomponent program with usual care for the treatment of depressed women in primary care in Santiago, Chile. METHOD A cost-effectiveness study was conducted of a previous randomized controlled trial involving 240 eligible women with DSM-IV major depression who were selected from a consecutive sample of adult women attending primary care clinics. The patients were randomly allocated to usual care or a multicomponent stepped-care program led by a nonmedical health care worker. Depression-free days and health care costs derived from local sources were assessed after 3 and 6 months. A health service perspective was used in the economic analysis. RESULTS Complete data were determined for 80% of the randomly assigned patients. After we adjusted for initial severity, women receiving the stepped-care program had a mean of 50 additional depression-free days over 6 months relative to patients allocated to usual care. The stepped-care program was marginally more expensive than usual care (an extra 216 Chilean pesos per depression-free day). There was a 90% probability that the incremental cost of obtaining an extra depression-free day with the intervention would not exceed 300 pesos (1.04 US dollars). CONCLUSIONS The stepped-care program was significantly more effective and marginally more expensive than usual care for the treatment of depressed women in primary care. Small investments to improve depression appear to yield larger gains in poorer environments. Simple and inexpensive treatment programs tested in developing countries might provide good study models for developed countries.
Cardiac transplantation in adults with congenital heart disease

Background Due to increasing success with repair or palliation in childhood, there is a rapidly growing population of adult patients with complex congenital heart disease who may require transplantation. There remains little data on outcomes of cardiac transplantation in this group. Methods 38 orthotopic cardiac transplants were performed in 37 patients (18 men) ≥18 years of age with congenital heart disease (CHD) from 1988 to 2009 in our institution. Outcomes were reviewed using medical records and transplant databases. Results 15 patients (41%) had univentricular and 22 (59%) biventricular physiology. The biggest group was transposition of the great arteries following atrial switch in eight patients (22%). Six (16%) had no previous surgical intervention. Mean age at transplant was 33.5 years (range 19.1–59.9 years). 11 patients (30%) required additional surgical procedures at transplant. 16 (43%) died, 12 early and 4 late deaths (1.8, 2.4, 2.7 and 7 years). Survival was 70% at 30 days, 68% at 1 year, 58% at 5 years and 53% at 10 and 15 years. Outcome improved in later eras with reduction in 30-day mortality from 50% to 18% and increase in 5-year survival from 50% to 69%. Two patients developed post-transplant lymphoproliferative disease. None required long-term renal replacement therapy. One patient was re-transplanted for cardiac allograft vasculopathy. Conclusions While operative mortality following cardiac transplantation for adult congenital heart disease is higher than for other diagnostic groups, long-term survival is good and comparable to patients without CHD. Disappointing early results are improved with increasing experience.
Relationships Between Sleep Spindles and Activities of Cerebral Cortex as Determined by Simultaneous EEG and MEG Recording

Summary: The purpose of this study was to clarify the relationships between the distributions and cortical sources of two types of spindles in the magnetoencephalogram (MEG) and how cortical activating areas contribute to the distribution of spindles. Spontaneous activities during sleep stage 2 were recorded from 7 normal subjects by simultaneous EEG and MEG recordings. Two types of spindles with frequency-specific topographic differences (fast spindles and slow spindles) were defined by EEG, and, subsequently, the sources of spindles were estimated as equivalent current dipoles using MEG. Activation centered in four areas, the precentral and postcentral areas in posterior frontal cortex and parietal cortex of each hemisphere. However, these areas were not always activated simultaneously. Fast spindles were associated with more frequent activation of postcentral areas with stronger activation strengths, whereas slow spindles were associated with more frequent activation of precentral areas with stronger activation strengths. When spindles were distributed symmetrically in amplitude between the hemispheres on both EEG and MEG, the four areas were activated equally and simultaneously. When spindles exhibited asymmetric distributions with amplitude differences above 30% between hemispheres, the cortical areas were activated with variable temporal relationships. Two types of spindle oscillations observed in the MEG had a common neural basis at the cortical level, with variability in patterns of activation and activation strengths resulting in the differences in distribution observed on the EEG and MEG. The differences in cortical activation patterns and activation strengths between the two types of spindles suggest that two distinct forms of spindle bursts propagate to cortex through different underlying neuronal circuits. Defining the cortical activating areas for spindles by MEG is valuable to consider the underlying neural basis.
Urban low carbon transitions: institution-building and prospects for interventions in social practice

ABSTRACT As we witness increasing numbers and range of low carbon experiments, attention inevitably turns to how they are sustained and whether they can generate more systemic change in carbon-related consumption. This paper responds to the ‘spatial turn’ in socio-technical transitions, and the ‘practice turn’ in social theory to consider the role of intermediary organizations in potential shifts from experiments to institutional arrangements favouring transitions. Through the example of Climate Change Alliances in Victoria, Australia, the paper examines how such intermediary organizations seek to experiment and in so doing contribute towards institution building. With a focus on the interstitial spaces between local authorities, regional firms, agencies, and state governments we speculate on the prospects for systemic change given the resources, positioning and social strategies of the Alliances as intermediary institution builders.
Dominant C‐terminal deletions of FtsZ that affect its ability to localize in Caulobacter and its interaction with FtsA

The cell division protein FtsZ is composed of three regions based on sequence similarity: a highly conserved N‐terminal region of ≈320 amino acids; a variable spacer region; and a conserved C‐terminal region of eight amino acids. We show that FtsZ mutants missing different C‐terminal fragments have dominant lethal effects because they block cell division in Caulobacter crescentus by two different mechanisms. Removal of the C‐terminal conserved region, the linker, and 40 amino acids from the end of the N‐terminal conserved region (FtsZΔC281) prevents the localization or the polymerization of FtsZ. Because two‐hybrid analysis indicates that FtsZΔC281 does not interact with FtsZ, we hypothesize that FtsZΔC281 blocks cell division by competing with a factor required for FtsZ localization or that it titrates a factor required for the stability of the FtsZ ring. The removal of 24 amino acids from the C‐terminus of FtsZ (FtsZΔC485) causes a punctate pattern of FtsZ localization and affects its interaction with FtsA. This suggests that the conserved C‐terminal region of FtsZ is required for proper polymerization of FtsZ in Caulobacter and for its interaction with FtsA.
Disease detection in crops using remote sensing images

This paper describes an automated diseases detection using remote sensing images. Agriculturists are facing loss due to various crop diseases. It becomes tedious to the cultivators to monitor the crops regularly when the cultivated area is huge (in acres). The most significant part of our research is early detection the disease as soon as it starts spreading on the top layer of the leaves using remote sensing images. This approach has two phases: first phase deals with training of healthy and as well as diseased datasets i.e.) the extraction of threshold values from the image, second phase deals with monitoring of crops and identification of particular disease using canny edge detection algorithm and histogram analysis and also intimate the agriculturists with an early alert message immediately.
Key Functional Regions in the Histone Variant H2A.Z C-Terminal Docking Domain

ABSTRACT The incorporation of histone variants into nucleosomes represents one way of altering the chromatin structure to accommodate diverse functions. Histone variant H2A.Z has specific roles in gene regulation, heterochromatin boundary formation, and genomic integrity. The precise features required for H2A.Z to function and specify an identity different from canonical H2A remain to be fully explored. Analysis of the C-terminal docking domain of H2A.Z in Saccharomyces cerevisiae using epistatic miniarray profile (E-MAP) uncovered nuanced requirements of the H2A.Z C-terminal region for cell growth when additional genes were compromised. Moreover, the H2A.Z(1–114) truncation, lacking the last 20 amino acids of the protein, did not support regular H2A.Z functions, such as resistance to genotoxic stress, restriction of heterochromatin in its native context, GAL1 gene activation, and chromatin anchoring. The corresponding region of H2A could fully rescue the strong defects caused by loss of this functionally essential region in the C terminus of H2A.Z. Despite the dramatic reduction in function, the H2A.Z(1–114) truncation still bound the H2A.Z deposition complex SWR1-C, the histone chaperone Chz1, and histone H2B. These data are consistent with a model in which retaining the variant in chromatin after its deposition by SWR1-C is a crucial determinant of its function.
Temporal Changes in Precipitation and Temperature and their Implications on the Streamflow of Rosi River, Central Nepal

Nepal has experienced recent changes in two crucial climatic variables: temperature and precipitation. Therefore, climate-induced water security concerns have now become more pronounced in Nepal as changes in temperature and precipitation have already altered some hydrological processes such as the river runoff in some river systems. However, the linkage between precipitation patterns and streamflow characteristics are poorly understood, especially in small rivers. We analysed the temporal trends of temperature, precipitation, and extreme indices of wet and dry spells in the Rosi watershed in Central Nepal, and observed the temporal patterns of the streamflow of the Rosi river. We also examined the linkages between the average and extreme climate indices and streamflow. We found that the area has warmed up by an average of 0.03 °C/year, and has seen a significant decline in precipitation. The dry spell as represented by the maximum length of the dry spell (CDD) and the magnitude of dryness (AII) has become more pronounced, while the wet spell as represented by the number of heavy rainfall days (R5D) and the precipitation intensity on wet days (SDII) has diminished significantly. Our analysis shows that recent changes in precipitation patterns have affected the streamflow of the Rosi river, as manifested in the observed decline in annual and seasonal streamflows. The decrease in the availability of water in the river is likely to have severe consequences for water security in the area.
Local environmental factors characterizing Ixodes ricinus nymph abundance in grazed permanent pastures for cattle

SUMMARY Although Ixodes ricinus ticks are mainly associated with woodland, they are also present in open habitat such as pastures. The distribution of nymphal I. ricinus was monitored by drag sampling the vegetation in May–June 2003 on 61 grazed permanent pastures for cattle located in central France. After selecting explanatory variables from among a set of 155, tick abundance was modelled on the perimeter of the pasture using a negative binomial model that took into account data overdispersion. An abundant tree layer at the perimeter of the pasture associated with a high humidity before sampling greatly enhanced the average number of captured I. ricinus nymphs. The presence of apple or cherry trees around the pasture perimeter, the presence of trees or bushes at the pasture edge, woodland around the pasture and a high number of I. ricinus nymphs in the nearest woodland to the pasture were also favourable to nymph abundance in the pasture. The study highlighted that woodland vegetation associated with humidity and the presence of attractive foraging areas for tick hosts around the pasture played a key role in the abundance of I. ricinus. Finally, the results raised the question of whether and how transfer of ticks between woodland and grazed pastures occurs.