Miracle-dz/UniFAR-FTUs huggingface dataset:
Platelet endothelial cell adhesion molecule‐1 modulates endothelial cell motility through the small G‐protein Rho

Platelet endothelial cell adhesion molecule‐1 (PECAM‐1), an immunoglobulin family vascular adhesion molecule, is involved in endothelial cell migration and angiogenesis (1, 2). We found that endothelial cells lacking PECAM‐1 exhibit increased single cell motility and extension formation but poor wound healing migration, reminiscent of cells in which Rho activity has been suppressed by overexpressing a GTPase‐activating protein (3). The ability of PECAM‐1 to restore wound healing migration to PECAM‐1‐deficient cells was independent of its extracellular domain or signaling via its immunoreceptor tyrosine‐based inhibitory motif. PECAM‐1‐deficient endothelial cells had a selective defect in RhoGTP loading, and inhibition of Rho activity mimicked the PECAM‐1‐deficient phenotype of increased chemokinetic single cell motility at the expense of coordinated wound healing migration. The wound healing advantage of PECAM‐1‐positive endothelial cells was not only Rho mediated but pertussis toxin inhibitable, characteristic of migration mediated by heterotrimeric G‐protein‐linked seven‐transmembrane receptor signaling such as signaling in response to the serum sphingolipid sphingosine‐1‐phosphate (S1P) (4, 5). Indeed, we found that the wound healing defect of PECAM‐1 null endothelial cells is minimized in sphingolipid‐depleted media; moreover, PECAM‐1 null endothelial cells fail to increase their migration in response to S1P. We have also found that PECAM‐1 localizes to rafts and that in its absence heterotrimeric G‐protein components are differentially recruited to rafts, providing a potential mechanism for PECAM‐1‐mediated coordination of S1P signaling. PECAM‐1 may thus support the effective S1P/RhoGTP signaling required for wound healing endothelial migration by allowing for the spatially directed, coordinated activation of Galpha signaling pathways. —Gratzinger, D. , Canosa, S. , Engelhardt, B. , Maori, J. A. Platelet endothelial cell adhesion molecule‐1 modulates endothelial cell motility through the small G‐protein Rho, FASEB J. 17, 1458–1469 (2003)
A 300 nanosecond search memory

This paper describes a 24 digit, 128 word, transistorized magnetic thin film search memory with a 300 nanosecond read cycle time. With further improvement such a memory could work with a 100 nanosecond cycle time. A search or associative memory is one in which an input word is compared simultaneously with all stored words; an output is produced on all words that differ from the input word. Variations of this memory permit search between limits by setting up a "don't care" condition for some of the stored bits; however, the emphasis in this paper is on the equality search. The writing system described is capable of doing a complete rewrite of all 128 words in 13 milliseconds; much faster writing systems are possible. Applications are in sorting, cataloging, information retrieval, translation, and searching.
Bioenergetic requirements of a Tat‐dependent substrate in the halophilic archaeon Haloarcula hispanica

Twin‐arginine translocase (Tat) is involved in the translocation of fully folded proteins in a process that is driven by the proton motive force. In most prokaryotes, the Tat system transports only a small proportion of secretory proteins, and Tat substrates are often cofactor‐containing proteins that require folding before translocation. A notable exception is found in halophilic archaea (haloarchaea), which are predicted to secrete the majority of their proteins through the Tat pathway. In this study, we have analysed the translocation of a secretory protein (AmyH) from the haloarchaeon Haloarcula hispanica. Using both in vivo and in vitro translocation assays, we demonstrate that AmyH transport is Tat‐dependent, and, surprisingly, that its secretion does not depend on the proton motive force but requires the sodium motive force instead.
Redescription of the Blind Snake Anomalepis colombia (Serpentes: Anomalepididae) Using High-Resolution X-Ray Computed Tomography

The blind snake Anomalepis colombia was described based on a single specimen, and since the collection of this specimen by Kjell von Sneidern in January 1946, there are no records of other specimens in scientific collections. This species is known only from the type locality in La Selva, Pueblo Rico, Caldas, Colombia. In this study A. colombia is redescribed based on external morphology and a detailed osteological description of the skull, prepared by high-resolution x-ray computed tomography.
VLSI implementation of a tree searched vector quantizer

The VLSI design and implementation of a tree-searched vector quantizer is presented. The number of processors needed is equal to the depth of the tree. All processors are identical, and data flow between processors is regular. No global control signals are needed. The processors have been fabricated using 2 mu m N-well process on a 7.9*9.2 mm die. Each processor chip contains 25000 transistors and has 84 pins. The processors have been thoroughly tested at a clock frequency of 20 MHz. >
Reduced response of insecticide-resistant aphids and attraction of parasitoids to aphid alarm pheromone; a potential fitness trade-off

Abstract Response to the alarm pheromone, (E)-β-farnesene, produced by many species of aphids, was assessed in laboratory bioassays using an aphid pest, Myzus persicae (Sulzer), and its primary endoparasitoid, Diaeretiella rapae (McIntosh). This was done in three separate studies, the first investigating responses of a large number of M. persicae clones carrying different combinations of metabolic (carboxylesterase) and target site (kdr) insecticide resistance mechanisms, and the other two investigating the responses of young virgin female adult parasitoids. In M. persicae, both insecticide resistance mechanisms were associated with reduced repellence suggesting that each has a pleiotropic effect on aphid behaviour. In contrast, D. rapae females were attracted to the alarm pheromone source. The implications of this apparent fitness trade-off for the evolution and dynamics of insecticide resistance, and the potential for using beneficial insects to combat resistance development are discussed.
Regulation of EPC‐1/PEDF in normal human fibroblasts is posttranscriptional

The EPC‐1 (early population doubling level cDNA‐1) gene, also known as pigment epithelium‐derived factor, encodes a protein belonging to the serine protease inhibitor (serpin) superfamily that has been reported to inhibit angiogenesis and proliferation of several cell types. We have previously reported that the EPC‐1 mRNA and the secreted EPC‐1 protein are expressed at levels more than 100‐fold higher in early passage, G0, WI‐38 cells compared to either proliferating or senescent WI‐38 fibroblasts. To examine the molecular mechanisms that regulate changes in EPC‐1 gene expression in WI‐38 cells, we isolated and characterized the human EPC‐1 gene and determined the mRNA cap site. Transcriptional assays showed no change in the transcription rates of EPC‐1 between young proliferating, quiescent, and senescent WI‐38 cells. These results suggest posttranscriptional regulation of the EPC‐1 gene. Reverse transcriptase polymerase chain reaction measurements (of hnRNA) indicate regulation at the hnRNA level. The regulation of the EPC‐1 gene at the level of hnRNA can explain the observed slow increase in the steady‐state EPC‐1 mRNA levels when cells become quiescent. The reduction of EPC‐1 mRNA levels that occurs when cells exit G0 and are induced to proliferate can be accounted for by a reduction of the EPC‐1 mRNA stability in stimulated cells as compared to quiescent cells. J. Cell. Biochem. 79:442–452, 2000. © 2000 Wiley‐Liss, Inc.
Fractional Programming for Energy Efficient Power Control in Uplink Massive MIMO Systems

Recently, massive multiple input multiple output (MIMO) is considered as a promising technology to significantly improve the spectral efficiency (SE) and energy efficiency (EE) of fifth generation (5G) networks. Effective control of the transmit power and other network resources helps to maximize energy efficiency of massive MIMO systems. In this work, an energy efficient power control algorithm is proposed for uplink massive MIMO systems with zero forcing (ZF) detection and imperfect channel state information (CSI) at a base station (BS). By using large system analysis, we first derive closed-form lower bound spectral efficiency expression. Then, by utilizing methods from fractional programming theory, an energy efficient power control algorithm is derived. Numerical results validate the effectiveness of the proposed power control algorithm and show the impacts of maximum transmitter power and minimum rate constraints on energy efficiency maximization.
Incidence of Antibiotic Resistance in Coliforms from Drinking Water and Their Identification Using the Biolog and the API Identification Systems

Abstract Antibiotic-resistant bacteria were common in samples collected from an intermittent water distribution system in Lebanon. Multiply-resistant isolates were also present and most commonly to amoxycillin, cephalexin and sulfamethoxazole/trimethoprim. The aminoglycosides (amikacin, gentamicin and kanamycin) were the most effective, with almost all tested strains showing susceptibility to these antimicrobial agents. Both the Biolog GN MicroPlates and the API 20E strips can be used for the identification of coliform bacteria isolated from potable water, but the outcome of the identification should be viewed with caution. 51% of isolates were assigned similar identities by both the Biolog MicroPlates and the API 20E strips. The similarity at the species level was lower (33%) compared to that at the genus level (67%). The identification of Escherichia coli strains, which represented 30% of all tested organisms, showed 95% similarity in the assigned genus and species using both identification schemes.
Low Reserve of Cytochrome c Oxidase Capacity in Vivo in the Respiratory Chain of a Variety of Human Cell Types*

The question of whether and to what extent thein vivo cytochrome c oxidase (COX) capacity in mammalian cells exceeds that required to support respiration is still unresolved. In the present work, to address this question, a newly developed approach for measuring the rate of COX activity, either as an isolated step or as a respiratory chain-integrated step, has been applied to a variety of human cell types, including several tumor-derived semidifferentiated cell lines, as well as specialized cells removed from the organism. KCN titration assays, carried out on intact uncoupled cells, have clearly shown that the COX capacity is in low excess (16–40%) with respect to that required to support the endogenous respiration rate. Furthermore, measurements of O2 consumption rate supported by 0.4 mmtetramethyl-p-phenylenediamine in antimycin-inhibited uncoupled intact cells have given results that are fully consistent with those obtained in the KCN titration experiments. Similarly, KCN titration assays on digitonin-permeabilized cells have revealed a COX capacity that is nearly limiting (7–22% excess) for ADP + glutamate/malate-dependent respiration. The present observations, therefore, substantiate the conclusion that the in vivo control of respiration by COX is much tighter than has been generally assumed on the basis of experiments carried out on isolated mitochondria. This conclusion has important implications for understanding the role of physiological or pathological factors in affecting the COX threshold.
Genetic variation in neuregulin1 is associated with differences in prefrontal engagement in children

The majority of psychopathology is rooted early in life and first emerges during childhood and adolescence. However, little is known about how risk genes affect brain function to increase biological vulnerability to psychopathology in childhood, because most imaging genetic studies published so far have been conducted on adult participants. We examined the impact of neuregulin1 (NRG1), a probable susceptibility gene for schizophrenia and bipolar disorder, on brain function in a sample of 102 ten‐ to twelve‐year‐old children. Each participant performed a Go/Nogo task, whereas brain responses were measured using functional magnetic resonance imaging. Statistical parametric mapping was used to estimate the impact of genetic variation in NRG1 on brain activation. Response accuracy and reaction times did not differ as a function of NRG1 genotype. However, individuals with the high‐risk variant expressed greater brain activation for both Go and Nogo stimuli in the right posterior orbital gyrus, where NRG1 genotype accounted for 11% of interindividual variance. There were no regions showing a significant interaction between NRG1 genotype and stimulus type even at trend level, suggesting that the impact of NRG1 on brain activation was not specific to either response inhibition or motor execution. These results suggest that that genetic variation in NRG1 is associated with different levels of prefrontal engagement in children as young as 10–12 years of age. Our investigation provides support to the idea that genetic factors may affect brain function to moderate vulnerability to psychopathology from childhood. Hum Brain Mapp, 2009. © 2009 Wiley‐Liss, Inc.
Coexpression and estrogen‐mediated regulation of TRPV6 and PMCA1 in the human endometrium during the menstrual cycle

Maintenance of calcium balance in the uterus is essential for many of its functions, including embryo implantation. The plasma membrane Ca2+‐pumping ATPase proteins are encoded by four genes designated PMCA1‐4, and PMCA1 is expressed in the uterus of rats during the estrous cycle. Although transient receptor potential cation channel subfamily V, member 6 (TRPV6), has been detected in the human placenta, pancreas and the prostate gland, expression patterns of uterine TRPV6 and PMCA1 and their potential roles in the human endometrium remain to be elucidated. In the present study, the expression patterns of TRPV6 and PMCA1 were examined to predict their potential roles in the human endometrium during the menstrual cycle. Human classified endometrial tissues (total n = 40) were separated into three groups according to menstrual cycle phase: menstrual, proliferative (early‐, mid‐, late), and secretory phase (early‐, mid‐, late). The expression of TRPV6 and PMCA1 mRNA and protein in the uterine endometrium during the menstrual cycle increased by 1.5‐ to 1.8‐fold at the proliferative phase (early‐, mid‐, and late‐) in comparison to the other phases. Estrogen treatment caused a significant increase in TRPV6 and PMCA1 mRNA expression. Immunohistochemical analysis of the distribution of TRPV6 and PMCA1 in the uterus revealed that both proteins are abundantly expressed in the cytoplasm of endometrial and glandular epithelial cells during menstrual phases. Taken together, these results suggest that uterine expression of TRPV6 and PMCA1 may be involved in human reproductive function. Mol. Reprod. Dev. 78:274–282, 2011. © 2011 Wiley‐Liss, Inc.
Desulfuribacillus stibiiarsenatis sp. nov., an obligately anaerobic, dissimilatory antimonate- and arsenate-reducing bacterium isolated from anoxic sediments, and emended description of the genus Desulfuribacillus.

A novel anaerobic, Gram-stain-negative, endospore-forming bacterium, designated strain MLFW-2T, was isolated from anoxic sediments collected from the drainage area of a geothermal spring near Mono Lake, CA, USA. Optimal growth was achieved at 34 °C and pH 8.25-8.50 in medium containing 0.75 % (w/v) NaCl. Catalase, but not oxidase, was produced. Strain MLFW-2T was an obligate anaerobe capable of respiring with nitrate, nitrite, DMSO, arsenate, antimonate, selenate and selenite as terminal electron acceptors. Lactate, pyruvate, formate and H2 could serve as electron donors to support growth. The isolate was incapable of fermentation. The predominant fatty acids were C16 : 0, C16 : 1ω9c, C16 : 1ω7c, C18 : 1ω9c and C18 : 1ω7c. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The only isoprenoid quinone detected was menaquinone 7 (MK-7). The DNA G+C content was 38.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence demonstrated that strain MLFW-2T was a member of the order Bacillales and was most closely related to Desulfuribacillus alkaliarsenatis AHT28T (93.9 % similarity). On the basis of phenotypic and phylogenomic evidence, strain MLFW-2T represents a novel species of the genus Desulfuribacillus, for which the name Desulfuribacillus stibiiarsenatis sp. nov. is proposed. The type strain is MLFW-2T (=DSM 28709T=JCM 30866T). An emended description of the genus Desulfuribacillus is also provided.
Efficient computational models for optical nanowires

The numerical simulation of nanowires in the optical regime (400–790 THz) requires the accurate incorporation of appropriate electromagnetic constitutive material parameters. In this paper we utilize phenomenological models based on a Lorentz-Drude damped oscillator for describing the optical properties of an arbitrary solid [1]. A computationally efficient method of including these constitutive parameters in a full-wave electromagnetic solver is presented through the use of equivalent circuit models and integral equations. These models can be represented as equivalent electric circuits which can be simulated with both frequency-domain [2] and time-domain [3] EFIE formulations. Integral equation solvers using these models are very efficient in terms of memory and time in comparison with other full-wave solvers such as the FDTD method. It is demonstrated that nanowires at near infrared and the lower part of the visible spectrum can be accurately simulated with integral equations [4].
Localization and Activation of the ARF6 GTPase during Cleavage Furrow Ingression and Cytokinesis*

The ARF6 GTPase mediates cell shape changes in interphase cells through its effects on membrane cycling and actin remodeling. In this study, we focus our attention on the dynamics of cell division and present evidence supporting a novel role for ARF6 during cleavage furrow ingression and cytokinesis. We demonstrate that endogenous ARF6 redistributes during mitosis and concentrates near the cleavage furrow during telophase. Constitutively activated ARF6 localizes to the plasma membrane at the site of cleavage furrow ingression and midbody formation, and dominant negative ARF6 remains cytoplasmic. By using a novel pull-down assay for ARF6-GTP, we find an abrupt, but transient, increase in ARF6-GTP levels as cells progress through cytokinesis. Whereas high levels of expression of a GTPase-defective ARF6 mutant induce aberrant phenotypes in cells at cytokinesis, cells expressing low levels of ARF6 mutants do not display a significant mitotic delay or cytokinesis defect, presumably due to compensatory or redundant mechanisms that allow cytokinesis to proceed when the ARF6 GTPase cycle is disrupted. Finally, actin accumulation and phospholipid metabolism at the cleavage furrow are unchanged in cells expressing ARF6 mutants, suggesting that ARF6 may be involved in membrane remodeling during cytokinesis via effector pathways that are distinct from those operative in interphase cells.
Intra‐operative management of low portal vein flow in pediatric living donor liver transplantation

For pediatric living donor liver transplantation, portal vein complications cause significant morbidity and graft failure. Routine intra‐operative Doppler ultrasound is performed after graft reperfusion to evaluate the flow of portal vein. This retrospective study reviewed 65 children who had undergone living donor liver transplantation. Seven patients were detected with suboptimal portal vein flow velocity following vascular reconstruction and abdominal closure. They underwent immediate on‐table interventions to improve the portal vein flow. Both surgical and endovascular modalities were employed, namely, graft re‐positioning, collateral shunt ligation, thrombectomy, revision of anastomosis, inferior mesenteric vein cannulation, and endovascular stenting. The ultrasonographic follow‐up assessment for all seven patients demonstrated patent portal vein and satisfactory flow. We reviewed our experience on the different modalities and proposed an approach for our future intra‐operative management to improve portal vein flow at the time of liver transplantation.
Statistical information of crosstalk on parallel bus

There are lots of studies regarding methods of coding the information sent via a parallel data bus from inside a chip. As a result of shrinking the gadgets, the traces from an off-chip bus are getting closer, and the coupling capacitance is increasing. The traces from an off-chip bus are much longer than the ones from an on-chip bus, so a new study is necessary to analyze how crosstalk affects off-chip parallel data bus. The paper will take into account the capacitive coupling, but also the inductive coupling, which has bigger values for off-chip traces, affecting even more distant traces from the aggressor net. Additionally will be analyzed the effects of inter-symbol interference (ISI) on the crosstalk that appears between the traces of the parallel data bus. The study will show that methods used in coding theory for on-chip busses can't guarantee an error free transmission.
Wenzhouxiangella sediminis sp. nov., isolated from coastal sediment.

A novel Gram-stain-negative, non-spore-forming, non-motile, facultatively anaerobic, rod-shaped strain, designated XDB06T, was isolated from coastal sediment of Xiaoshi Island, Weihai, China. Optimal growth occurred at 37 °C, pH 7.5 and with 4.0 % (w/v) NaCl. Q-8 was the sole respiratory quinone. The major cellular fatty acids in strain XDB06T were iso-C15 : 0 and iso-C16 : 0. The polar lipids of strain XDB06T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified glycolipids and four unidentified phospholipids. The genomic DNA G+C content of the strain was 65.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XDB06T clusters within the genus Wenzhouxiangella and is most closely related to Wenzhouxiangella. marina MCCC 1K00261T, with a 16S rRNA gene sequence similarity of 96.50 %. Based on data from the current polyphasic study, strain XDB06T represents a novel species of the genus Wenzhouxiangella, for which the name Wenzhouxiangella sediminis sp. nov. is proposed. The type strain is XDB06T (=KCTC 52041T=MCCC 1K02285T).