Miracle-dz/UniFAR-FTUs huggingface dataset:
Direct observation of impact propagation and absorption in dense colloidal monolayers

Significance Single-particle characterization of the impact response has unveiled design principles to focus and control stress propagation in macroscopic granular crystalline arrays. We demonstrate that similar principles apply to aqueous monolayers of microparticles excited by localized mechanical pulses. By inducing extreme local deformation rates and tracking the motion of each particle with velocities that reach up to few meters per second, we reveal that a regime of elastic collisions, typically forbidden due to overdamping, becomes accessible. This provides insights on the stress propagation and energy absorption of dense suspensions upon fast deformation rates. Dense colloidal suspensions can propagate and absorb large mechanical stresses, including impacts and shocks. The wave transport stems from the delicate interplay between the spatial arrangement of the structural units and solvent-mediated effects. For dynamic microscopic systems, elastic deformations of the colloids are usually disregarded due to the damping imposed by the surrounding fluid. Here, we study the propagation of localized mechanical pulses in aqueous monolayers of micron-sized particles of controlled microstructure. We generate extreme localized deformation rates by exciting a target particle via pulsed-laser ablation. In crystalline monolayers, stress propagation fronts take place, where fast-moving particles (V approximately a few meters per second) are aligned along the symmetry axes of the lattice. Conversely, more viscous solvents and disordered structures lead to faster and isotropic energy absorption. Our results demonstrate the accessibility of a regime where elastic collisions also become relevant for suspensions of microscopic particles, behaving as “billiard balls” in a liquid, in analogy with regular packings of macroscopic spheres. We furthermore quantify the scattering of an impact as a function of the local structural disorder.
Comparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma.

CONTENTS Pregnancy-associated glycoproteins (PAGs) constitute a large family of glycoproteins that are synthesized in the superficial layer of the ruminant placenta according to a spatial and temporal expression pattern. When PAGs are released in the maternal blood they can be used for pregnancy diagnosis, pregnancy follow-up and for the monitoring of the trophoblastic function. Three different radioimmunoassay systems (RIA 1, RIA 2 and RIA 3) using antisera produced against PAG I67 (RIA 1), PAG55+62 (RIA 2) and PAG55+59 (RIA 3) were used in this investigation in order to measure the PAG concentration in plasma samples withdrawn from pregnant cows and heifers during different periods following artificial insemination (AI). These systems were able to detect PAG molecules in the maternal blood as early as 21 days after AI in different concentrations (RIA 1: 0.43 +/- 0.24 ng/ml, mean +/- SD; RIA 2: 0.48 +/- 0.24 ng/ml; RIA 3: 0.64 +/- 0.37 ng/ml). On days 32 and 42 RIA 2 (4.30 +/- 1.32 ng/ml and 5.56 +/- 1.95 ng/ml) and RIA 3 (4.17 +/- 1.15 ng/ml and 5.60 +/- 1.89 ng/ml) presented significantly (p < 0.0001) higher PAG concentrations than those of RIA 1 (2.43 +/- 0.81 ng/ml and 4.01 +/- 1.48 ng/ml), respectively. After day 21, significant correlations (p < 0.0001; r >/= 0.929) were determined between the three systems. Additionally the three individual PAG profiles presented in this study showed that PAG molecules secreted in the maternal blood between 21 and 50 days after AI were better recognized by the RIA 2 and RIA 3 systems. This study clearly indicated that the ability of a RIA test to recognize PAG molecules in the maternal blood can be improved by carefully selecting the antiserum.
Energy Balancing Improvement of Modular Multilevel Converters Under Unbalanced Grid Conditions

This paper presents a feedback/feed-forward control strategy to improve the voltage balancing of modular multilevel converters (MMCs) under unbalanced grid conditions. The inclusion of a feed-forward compensation improves the disturbance rejection capability against asymmetrical faults and sudden voltage imbalances in the ac grid. The floating capacitor voltages of the MMC are controlled in two stages. The first one equally distributes the voltages in each arm, and the second one balances the energy among the six arms. This last control stage is also divided into the called horizontal and vertical balancing. Based on a detailed analysis of the power terms disturbing the MMC arm energies, the control strategy is tailored to improve the voltage transient response by adding feed-forward terms to both horizontal and vertical balancing controls. In addition, various approaches to manage the MMC under unbalanced grid conditions are compared regarding several aspects, such as capacitor voltage ripple, balancing performance, and negative-sequence current injection. Advantages and disadvantages of both the proposed and the conventional energy-based control schemes are also studied, showing that the voltage control performance can be improved by enhancing the existing MMC control systems.
Postsynaptic activation at the squid giant synapse by photolytic release of L‐glutamate from a ‘caged’ L‐glutamate.

1. Pharmacological evidence suggests L‐glutamate is a strong candidate as a transmitter at the giant synapse of the squid. Postsynaptic activation at the giant synapse cannot be effected by conventional application of putative neurotransmitters by iontophoresis or perfusion, apparently because the complex structure of the synapse prevents a sufficiently rapid change in concentration at the postsynaptic membrane. Flash photolytic release of L‐glutamate from a pharmacologically inert ‘caged’ L‐glutamate pre‐equilibrated in the stellate ganglion of Alloteuthis or Loligo was used to determine whether L‐glutamate can produce postsynaptic activation when released rapidly in the synaptic clefts. 2. The preparation, reaction mechanism and properties of the caged L‐glutamate, N‐1‐(2‐nitrophenyl)ethoxycarbonyl‐L‐glutamate, are described. The product quantum yield on photolysis was 0.65 (+/‐ 0.05). On flash photolysis glutamate release followed a single exponential time‐course in the pH range 5.5‐7.8. The rate constant was proportional to [H+] and was 93 s‐1 at pH 5.5 and 16 degrees C in artificial sea water (ionic strength, I = 0.68 M). 3. At pH 7.8 flash photolysis of caged glutamate pre‐equilibrated in the synapse caused only a slow depolarization. A second photolytic release of L‐glutamate or transsynaptic activation produced no further depolarization, suggesting desensitization and inactivation of postsynaptic mechanisms by the initial pulse of L‐glutamate. 4. Synaptic transmission in the giant synapse was normal at pH 5.5. Flash photolysis at pH 5.5 caused rapid production of L‐glutamate within the synaptic cleft and a fast postsynaptic depolarization which generated postsynaptic action potentials. (ABSTRACT TRUNCATED AT 250 WORDS)
POSTERIOR CERVICAL SPINE SURGERY FOR RADICULOPATHY

IT IS NOW common knowledge that cervical radiculopathy, frequently caused by disc herniation and/or degeneration, will often improve without surgical intervention. Only a small percentage of patients with the severity of symptoms necessitate surgical treatment. Surgery for radiculopathy is indicated for motor weakness, progressive neurological deficits, and progressive symptoms that do not improve with nonoperative treatment. Advantages and disadvantages exist for both ventral and dorsal approaches in the surgical treatment of cervical radiculopathy. Indications and results for dorsal nerve root decompression are discussed, and a review of our preferred techniques, including use of minimally invasive technology, is presented.
Cutting Edge: Requirement of MARCH-I-Mediated MHC II Ubiquitination for the Maintenance of Conventional Dendritic Cells1

MARCH-I (membrane-associated RING-CH I) has been suggested as a physiological E3 ubiquitin ligase for both MHC class II (MHC II) and B7-2. In this study, we show that MARCH-I-mediated MHC II ubiquitination is necessary for the maintenance of conventional dendritic cell (cDC) functions in the steady state. MARCH-I-deficient cDCs accumulated MHC II and B7-2 and exhibited low Ag-presenting ability for exogenous Ags and low cytokine-producing ability upon stimulation in vivo. Importantly, MHC II, but not B7-2, was required for impaired cDC function induced by loss of MARCH-I in vivo. Moreover, MHC II knockin mice whose MHC II was not ubiquitinated showed dysfunction of cDC similar to that of MARCH-I knockout mice. These results suggest that the accumulation of MHC II resulting from loss of ubiquitination caused cDC abnormality; therefore, MARCH-I may function as a housekeeper of cDC in the steady state.
Volatility of Treasury bond futures Price: evidence from Tick-by-Tick data

In order to find the causes of price volatility in bond futures market, we consider the potential impact of trading properties and construct a multivariate linear model between realized volatility and Behavioral variables included the strategies of Open, Close, Long, Short and turnover. Then through an empirical analysis of tick-by-tick data, we found that realized volatility is negative respectively related to Long, Double-close and turnover, and positive correlated to Long-Close, Shot and shot-Close. The double-turnover has weakly influence, and both sides explain differences. The volume is not significantly explaining the price volatility. Therefore, our findings will be helpful to manage the risk of bond futures transactions.
Do Football Consumers Care About Sport Governance? An Analysis of Social Media Responses to the Recent FIFA Scandal

ABSTRACT Given the fact that the governing body of world football, FIFA, has managed to survive major failures in sports governance, this paper addresses the role of football consumers as potential principals. By characterizing FIFA as a ‘political machine’ and using ‘digital trace data’ from Twitter to analyze responses to the most recent FIFA corruption scandal, this paper introduces a new methodological approach to the realm of research on sports governance. Key events of FIFA's corruption scandal trigger significant activities, but the attention is ephemeral as it decreases over time. In addition, there is little evidence that the corruption scandal has a detrimental effect on FIFA's legitimacy among users. Ultimately, online activism striving to change FIFA's governance seems to have only rudimentarily developed and is sparsely popular among individual football fans. The paper casts further doubts on the ability of football consumers to act as effective principals in football governance.
Using Multiliteracies to Engage and Empower Students with Complex Support Needs

i SIGNATURE PAGE . iii ACKNOWLEDGEMENTS . iv DEDICATION . v LIST OF TABLES . vii LIST OF FIGURES . ix Article Page I. CREATING NEW LEARNING SPACES USING MULTILITERACIES WITH STUDENTS WITH COMPLEX SUPPORT NEEDS .
Hepatitis C Virus Induces Epithelial-Mesenchymal Transition in Primary Human Hepatocytes

ABSTRACT Hepatitis C virus (HCV)-mediated liver disease progression may reflect distinct molecular mechanisms for increased hepatocyte growth and hepatic stellate cell activation. In this study, we have observed that primary human hepatocytes, when infected in vitro with cell culture-grown HCV genotype 1a or 2a, display viral RNA and protein expression. Infected hepatocytes displayed a fibroblast-like shape and an extended life span. To understand the changes at the molecular level, we examined epithelial-mesenchymal transition (EMT) markers. Increased mRNA and protein expression levels of vimentin, snail, slug, and twist and a loss of the epithelial cell marker E-cadherin were observed. Snail and twist, when examined separately, were upregulated in chronically HCV-infected liver biopsy specimens, indicating an onset of an active EMT state in the infected liver. An increased expression level of fibroblast-specific protein 1 (FSP-1) in the infected hepatocytes was also evident, indicating a type 2 EMT state. Infected hepatocytes had significantly increased levels of phosphorylated β-catenin (Ser552) as an EMT mediator, which translocated into the nucleus and activated Akt. The phosphorylation level of β-catenin at Thr41/Ser45 moieties was specifically higher in control than in HCV-infected hepatocytes, implicating an inactivation of β-catenin. Together, these results suggested that primary human hepatocytes infected with cell culture-grown HCV display EMT via the activation of the Akt/β-catenin signaling pathway. This observation may have implications for liver disease progression and therapeutic intervention strategies using inhibitory molecules.
The role of anti-Müllerian hormone assessment in assisted reproductive technology outcome

Purpose of review The purpose of this study is to summarize the role of anti-Müllerian hormone (AMH) in assisted reproductive technology (ART) treatment. Recent findings AMH is a good marker in the prediction of ovarian response to controlled ovarian hyperstimulation. In clinical practice, this means that AMH may be used for identifying poor or excessive responders. So far, studies show that AMH is not a good predictor for the occurrence of pregnancy after ART treatment. Therefore, routine screening for a poor ovarian reserve status using AMH is not to be advocated. Still, ovarian response prediction using AMH may open ways for patient-tailored stimulation protocols in order to reduce cancellations for excessive response, possibly improve pregnancy prospects and reduce costs. Summary AMH is able to predict extremes in ovarian response to controlled ovarian hyperstimulation but cannot predict pregnancy after ART treatment. Its future clinical role may be in the individualization of ART stimulation protocols.
Meniscal regeneration with copolymeric collagen scaffolds

We sought to create a regeneration template for the meniscal cartilage of the knee to induce complete men iscal regeneration, and to develop the technique for implanting the prosthetic appliance in vivo. We de signed a resorbable collagen-based scaffold and con ducted in vitro and in vivo studies. In vivo, the scaffold was implanted in the knees of immature swine and mature canines and evaluated clinically, histologically, and biochemically. Because the canine stifle joint me niscus is more clinically relevant to the human menis cus, this paper emphasizes those results. We studied 24 mixed breed dogs (14 males and 10 females) with an average weight of 25.5 kg (range, 20 to 35) that were obtained from a USDA-licensed sup plier. The dogs were deemed clinically and radiograph ically skeletally mature. None of the dogs had a preex isting knee joint abnormality. All dogs underwent an 80% subtotal resection of the medial meniscus bilater ally. A collagen template was implanted in one stifle (N = 24). The contralateral side served as a control: 12 dogs had a total resection alone and the other 12 dogs had an immediate replantation of the autologous meniscus. Results were tabulated at 3, 6, 9, and 12 months. At final evaluation, before the animals were euthanized, the results were submitted for statistical analysis as well as histologic and biochemical analyses. The results demonstrated that a copolymeric collagen-based scaf fold can be constructed that is compatible with meniscal fibrochondrocyte growth in vitro and in vivo, that does not inhibit meniscal regeneration in an immature pig, and that may induce regeneration of the meniscus in the mature dog. Although additional studies are necessary to perfect the scaffold and to evaluate the implant in the environ ment of the knee, these studies suggest that effective meniscal regeneration can be supported by an im planted collagen-based scaffold designed to support cellular ingrowth.
The extended direct endonasal transsphenoidal approach for nonadenomatous suprasellar tumors.

OBJECT The extended transsphenoidal approach, which requires a bone and dural opening through the tuberculum sellae and posterior planum sphenoidale, is increasingly used for the treatment of nonadenomatous suprasellar tumors. The authors present their experiences in using the direct endonasal approach in patients with nonadenomatous suprasellar tumors. METHODS Surgery was performed with the aid of an operating microscope and angled endoscopes were used to assess the completeness of resection. Bone and dural defects were repaired using abdominal fat, collagen sponge, titanium mesh, and, in most cases, lumbar drainage of cerebrospinal fluid (CSF). Twenty-six procedures for tumor removal were performed in 24 patients (ages 9-79 years), including two repeated operations for residual tumor. Gross-total removal could be accomplished in only 46% of patients, with near-gross-total removal or better in 74% of 23 patients (five of eight with craniopharyngiomas, six of seven with meningiomas, five of six with Rathke cleft cysts, and one of two with a dermoid or epidermoid cyst); a patient with a lymphoma only underwent biopsy. Of 13 patients with tumor-related visual loss, 85% improved postoperatively. The complications that occurred included five patients (21%) with postoperative CSF leaks, one patient (4%) with bacterial meningitis; five patients (21%) with new endocrinopathy; and two patients (8%) who needed to undergo repeated operations to downsize suprasellar fat grafts. The only permanent neurological deficit was anosmia in one patient; there were no intracranial vascular injuries. CONCLUSIONS The direct endonasal skull-base approach provides an effective minimally invasive means for resecting or debulking nonadenomatous suprasellar tumors that have traditionally been approached through a sublabial or transcranial route. Procedures in the supraglandular space can be performed effectively with excellent visualization of the optic apparatus while preserving pituitary function in most cases. The major challenge remains developing consistently effective techniques to prevent postoperative CSF leaks.
The Histone Methyltransferase SDG724 Mediates H3K36me2/3 Deposition at MADS50 and RFT1 and Promotes Flowering in Rice[W][OA]

In rice, the two florigens Hd3a and RFT1 are separated by only 11.5 kb in the genome. This study reveals that rice SDG724 specifically affects the histone H3 lysine 36 me2/3 level of RFT1 but not its close paralog Hd3a. Therefore, RFT1 and Hd3a have functionally diverged to control flowering time under long-day and short-day conditions partly via a fine-tuned epigenetic mechanism. Chromatin modifications affect flowering time in the long-day plant Arabidopsis thaliana, but the role of histone methylation in flowering time regulation of rice (Oryza sativa), a short-day plant, remains to be elucidated. We identified a late-flowering long vegetative phase1 (lvp1) mutant in rice and used map-based cloning to reveal that lvp1 affects the SET domain group protein 724 (SDG724). SDG724 functions as a histone methyltransferase in vitro and contributes to a major fraction of global histone H3 lysine 36 (H3K36) methylation in vivo. Expression analyses of flowering time genes in wild-type and lvp1 mutants revealed that Early heading date1, but not Heading date1, are misregulated in lvp1 mutants. In addition, the double mutant of lvp1 with photoperiod sensitivity5 (se5) flowered later than the se5 single mutant, indicating that lvp1 delays flowering time irrespective of photoperiod. Chromatin immunoprecipitation assays showed that lvp1 had reduced levels of H3K36me2/3 at MADS50 and RFT1. This suggests that the divergent functions of paralogs RFT1 and Hd3a, and of MADS50 and MADS51, are in part due to differential H3K36me2/3 deposition, which also correlates with higher expression levels of MADS50 and RFT1 in flowering promotion in rice.
Portraits of Three Language Activists in Indigenous Language Reclamation

In an approach inspired by portraiture and ‘history in person,’ this paper portrays three women Indigenous language activists engaged in language reclamation, highlighting the mutually constitutive nature of language and the enduring struggles of Indigenous peoples that are crucibles for forging their identities. Neri Mamani breaks down longstanding language and identity compartmentalisations in Peru by assuming a personal language policy of using Quechua and engaging in Indigenous practices in public, urban, and literate spaces. Nobuhle Hlongwa teaches a university course on language planning through isiZulu medium and is a key figure in advocating for, negotiating, and implementing multilingual language policy at her university and in South Africa. Though discouraged by the politics of language policy, Hanna Outakoski stays in the fray for the sake of Sámi language, as university teacher of Sámi, activist for Sámi at the municipal level, and researcher in a cross-national multilingual literacy assessment of Sámi youth. Though the portraits give only a glimmer of the rich and complex lives, scholarship, and commitment of the three women, they demonstrate the power of individuals in shaping language landscapes, policy, and assessment; and the implementational and ideological paths and spaces for language reclamation opened up as they do so.
Sustainability of Italian Budgetary Policies: A Time Series Analysis (1862-2013)

In this paper, we analyze the sustainability of Italian public finances using a unique database covering the period 1862-2013. This paper focuses on empirical tests for the sustainability and solvency of fiscal policies. A necessary but not sufficient condition implies that the growth rate of public debt should in the limit be smaller than the asymptotic rate of interest. In addition, the debt-to-GDP ratio must eventually stabilize at a steady-state level. The results of unit root and stationarity tests show that the variables are non-stationary at levels, but stationary in first-differences form, or I(1) . However, some breaks in the series emerge, given internal and external crises (wars, oil shocks, regime changes, institutional reforms). Therefore, the empirical analysis is conducted for the entire period, as well as two sub‐periods (1862‐1913 and 1947‐2013). Moreover, anecdotal evidence and visual inspection of the series confirm our results. Furthermore, we conduct tests on cointegration, which evidence that a long-run relationship between public expenditure and revenues is found only for the first sub-period (1862-1913). In essence, the paper’s results reveal that Italy have sustainability problems in the Republican age.