Miracle-dz/UniFAR-FTUs huggingface dataset:
Immunofluorescence techniques in dermatopathology

The use of iinmunolluorescence in both diagnosis and research is well recognized in dermatology, dating from the description of the granular deposition of Ig and C3 along the dermo-epidermal junction in lesions of lupus erythematostis by Bumham et al. (1963), and the discovery of Beutner and Jordon (1964), who used an indirect immunofluorescent technique, to demonstrate the presence of antibodies to what was then considered to be the intercellular substance of stratified squamous cpithelia in the scrum of patients with pemphigus. Immunofluorescence techniques are an immunological method which may be used to demonstrate cellular antigens, either in tissue section or cell suspension. The fluorescent dyes used routinely are fluorescein atid rhodamine which can be coupled to antibodies without destroying the specificity of the latter. Non-antibody proteins are removed by washing, and the resultant antigen-antibody complex formed is visualized using the fluorescence microscope. This paper will discuss in detail the theory and practice of immunofluorescent techniques used in diagnostic and research laboratories. Historically, Coons et al. (1941) were one of the first groups to work on the use of chemically labelled antibodies as reagents for the detection of antigens in mammalian tissue. Such a method required the retention of specificity by the antibody molecule during and following necessary chemical manipulatioti, a stable chemical linkage between an antibody and its label, a label that could be detected in minute quantities, and easy separation of a labelled antibody solution from unconjugated tracer material. Coons et al. (1941) first synthesized a conjugate of P-anthryl isocyanate with anti-pneumococcus Type III rabbit scrum. This rendered Type III pneumococci fluorescent in ultraviolet light, whereas Type II organisms were unreactive. However, the resultant blue fluorescence was impractical since mammalian connective tissue normally exhibits a blue autofluorescence which is enhanced by formalin fixation. This group then attempted successfully to synthesize a fluorescein-conjugatcd antipneumococcal Type 111 antibody (Coons et al. 1942). Murine tissue which was heavily infected with pneumococcus Type III was incubated with the conjugate, resulting in an easily discernable, bright green fluorescence on ultraviolet microscopic examination. The reac-
Data system for assessing probability of failure in SME reorganization

Purpose – The purpose of this paper is to develop a data system to assess failure probability in small to medium‐sized enterprise (SME) reorganization.Design/methodology/approach – The data system is based on information from 83 reorganized Finnish SMEs. Information is divided into four types: pre‐filing non‐financial, pre‐filing financial, reorganization submission, and reorganization plan information. Partial least squares (PLS) analysis is used in data mining to factorize information for each type of information. Logistic regression analysis is applied to assess failure probability.Findings – Useful data system can be developed on the basis of pre‐filing non‐financial information to support reorganization decision. Pre‐filing financial information only marginally improves quality of information. Submission and reorganization plan information improve quality in terms of fit but do not significantly improve classification accuracy.Research limitations/implications – The sample is small and should be expa.
Sex, Sex-Roles, and Irrational Beliefs

Irrational beliefs have been related to a variety of psychological distresses, some of which are thought of more in relation to one sex than the other, i.e., depression, nonassertiveness, anger. However, Ellis did not assert that there were sex differences in irrational beliefs, and few researchers have examined the effect of sex-role socialization on irrational beliefs. The present study explored the effect of sex and sex-roles on irrational beliefs for a sample of 270 college students using the Irrational Beliefs Test and Bern's scale. While the study supports the belief that sex and sex-role are not unidimensional constructs, the data suggest that differences in sex-role socialization contribute to differences in adherence to irrational beliefs. It appears, however, that a feminine sex-role orientation is related to irrational beliefs. Although study is needed, clinicians are cautioned not to assume there are no sex or sex-role differences related to irrational thinking.
Kriz Yönetimi ve Kriz İletişimi Bağlamında Soma Maden Faciası Krizine Bakış

Kriz konusu ozellikle 21. Yuzyilda isletme yonetimi ve halkla iliskiler disiplinlerinin oncelikli calisma alanlarindan biri olmustur. Gerceklestirilen calismalarda krizlerin onceden sezilmesi, kriz aninda ve sonrasinda yapilmasi gerekenlerden yola cikilarak bircok calisma yapilmistir. Calismalar genellikle iki genel baslik altinda literaturde bir araya getirilmistir. Bu basliklar “kriz yonetimi” ve “kriz iletisimidir”. Kriz yonetimi isletmelere kriz boyunca yasanan krizin isletmeye olumsuz etkilerini nasil en aza indirilecegi konusu uzerine odaklanirken, kriz iletisimi ise daha cok kriz yonetimi esnasinda isletmelerin hedef kitlelerine mesajlarini nasil iletebilecegi konularina odaklanmistir. Bu makalede ilk olarak kriz iletisimi ve kriz yonetimi ile ilgili literaturdeki bazi modeller uzerinden bilgiler paylasilmistir. Calismanin uygulama kisminda ise 2014 Yili’nda Soma Maden Isletmeleri’ne ait maden ocaginda yasanan ve 301 iscinin hayatini kaybettigini facianin isletme yonetimi tarafindan kriz iletisimi ve kriz yonetimi ilkeleri dogrultusunda dogru ve yanlislari analiz edilmistir. Bu kriz halkla iliskiler disiplini acisindan irdelenmis ve isletmelerin kriz yonetimi ve iletisiminde dikkat etmesi gerekenlere yonelik bilgiler paylasilmistir.
Energy management unit managing energy sources for a farm

The paper presents the laboratory based model of an “Energy Management Unit” for the optimum use of the electricity produced from alternative sources like biogas plant and solar photovoltaic system at an agriculture farm. The electrical energy produced at the farm is distributed among the loads according to the availability and requirement. A diesel generator is used as a backup at the farm. If the power from photovoltaic (PV) system is sufficient, the loads are driven by electricity from PV system else the electricity from biogas drives the load. Arduino-microcontroller based circuit has been used to control the operation of the sources and loads. The sensors at the source monitor whether or not the available energy is enough to drive all the loads and sends the information to the microcontroller. The microcontroller then selects the loads that could be driven by the available power.
Multi-sensorial inputs for the identification of persons with synergetic computers

We present an approach to combine the optical motion analysis of the lips and acoustic voice analysis of defined single words for identifying the people speaking. Due to the independence of the different data sources, a higher reliability of the results in comparison with simple optical lip reading is observed. The classification of the preprocessed data is done by synergetic computers, which have attracted increasing attention as robust algorithms for solving industrial classification tasks. Special potential of synergetic computers lies in their close mathematical similarity to self-organized phenomena in nature. Therefore they present a clear perspective for hardware realizations. We propose that the combination of motion and voice analysis offers a possibility for realizing robust access control systems. <<ETX>>
Dictionary-based compressive SLAM

Obtaining a compact representation of a large-size datapoint map built by mapper robots is a critical issue for recent SLAM applications. This ”map compression” problem is explored from a novel perspective of dictionary-based map compression in the paper. The primary contribution of the paper is proposal of an incremental scheme for simultaneous mapping and map-compression applications. An incremental map compressor is presented by employing a modified RANSAC map-matching scheme as well as the compact projection technique. Experiments show promising results in terms of compression speed, compactness of data and structure, as well as an application to the compression distance.
Resource Allocation in Multicell OFDM Systems Based on Noncooperative Game

In this paper, we present a game-theoretical approach to allocate the power in multicell OFDM systems by a distributed way. A specific utility function is defined considering the users' average utility per power, i.e., power unit based utility. The resource control problem is formulated as a noncooperative game, with which the properties of the Nash equilibrium of the game is investigated. Since the equilibrium is Pareto inefficient, we propose a pricing policy to the user's transmit power by adding a penalty price. With the adoption of the price, the user's aggressive behavior is depressed and Pareto improvement is achieved. Simulation results show that the proposal outperforms the pure water-filling algorithm and the users obtain higher utility and lower transmit power by proper pricing policy
Adenovirus-mediated Overexpression of Liver 6-Phosphofructo-2-kinase/Fructose-2,6-bisphosphatase in Gluconeogenic Rat Hepatoma Cells

6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase has been postulated to be a metabolic signaling enzyme, which acts as a switch between glycolysis and gluconeogenesis in mammalian liver by regulating the level of fructose 2,6-bisphosphate. The effect of overexpressing the bifunctional enzyme was studied in FAO cells transduced with recombinant adenoviral constructs of either the wild-type enzyme or a double mutant that has no bisphosphatase activity or protein kinase phosphorylation site. With both constructs, the mRNA and protein were overexpressed by 150- and 40-fold, respectively. Addition of cAMP to cells overexpressing the wild-type enzyme increased the S0.5 for fructose 6-phosphate of the kinase by 1.5-fold but had no effect on the overexpressed double mutant. When the wild-type enzyme was overexpressed, there was a decrease in fructose 2,6-bisphosphate levels, even though 6-phosphofructo-2-kinase maximal activity increased more than 22-fold and was in excess of fructose-2,6-bisphosphatase maximal activity. The kinase:bisphosphatase maximal activity ratio was decreased, indicating that the overexpressed enzyme was phosphorylated by cAMP-dependent protein kinase. Overexpression of the double mutant resulted in a 28-fold increase in kinase maximal activity and a 3-4-fold increase in fructose 2,6-bisphosphate levels. Overexpression of this form inhibited the rate of glucose production from dihydroxyacetone by 90% and stimulated the rate of lactate plus pyruvate production by 200%. In contrast, overexpression of the wild-type enzyme enhanced glucose production and inhibited lactate plus pyruvate production. These results provide direct support for fructose 2,6-bisphosphate as a regulator of gluconeogenic/glycolytic pathway flux and suggest that regulation of bifunctional enzyme activities by covalent modification is more important than the amount of the protein.
Activity of clarithromycin compared with those of other drugs against Mycobacterium paratuberculosis and further enhancement of its extracellular and intracellular activities by ethambutol

Radiometric MICs of clarithromycin, a new macrolide drug, were determined against five mycobactin-dependent strains of Mycobacterium paratuberculosis (including two Crohn's disease clinical isolates) and compared with those of other drugs which included rifampin, ethambutol, amikacin, ofloxacin, ciprofloxacin, and sparfloxacin. Among the drugs screened, clarithromycin was the drug for which MICs were lowest against the five strains tested. As MICs were significantly below the reported Cmax levels (about 4 micrograms/ml), the intracellular activity of clarithromycin against the type strain of M. paratuberculosis maintained in cultured macrophages was screened. Clarithromycin was able to kill the initial inoculum by more than 1 log within 7 days, and this activity was further potentiated by ethambutol. Extracellular drug combination screened by using sublethal concentrations of the drugs showed that ethambutol was able to enhance clarithromycin activity in three out of four M. paratuberculosis strains instead of only one out of four strains (or none in the case of ofloxacin) when associated with other drugs. These results suggest that clarithromycin may be fruitful to treat human disease in which M. paratuberculosis may be etiologically involved.
A Research Framework for Business Sustainability using Information Exchange - A Firm Centric Perspective

The information systems research literature associated with sustainability has focused, by and large, on the ecological effects of computer manufacturing, components, toxicity of materials, energy usage in the manufacturing of computers, energy usage by deployed computers, and safe disposal of old machines. There has been scant or no attention paid to the effective use of information by firms for making the firm sustainable while also paying attention to the triple bottom line (3BL). In this paper, we identify the research gaps, which when filled can influence and affect information deployment practices in business settings, to proactively assist firms in becoming sustainable and competitive within their business environments, while meeting their 3BL goals. We recognize the need for an entity or enterprise, designated the Information Enterprise (IE) that is the repository and sole distributor of relevant information that participant firms will need for achieving sustainability without sacrificing their 3BL goals.
Tissue-engineering acellular scaffolds-The significant influence of physical and procedural decellularization factors.

The importance of decellularized medical products has significantly increased during the last years. In this paper, we evaluated the effects of selected physical and procedural decellularization (DC) factors with the aim to systematically assess their influence on DC results. 72 porcine aortic walls (AW) were divided into three groups and exposed to a DC solution for 4 h and 8 h, either continuously or in repeated cycles. The AW were rocked (90bpm), whirled (10 l/min), sonicated (120W, 45 kHz) or exposed to a combination of these treatments, followed by 10 washing cycles. Defining successful DC as removal of nuclei while keeping an intact extracellular matrix (ECM), we equalized the efficiency to the penetration depth (PD), obtained by DAPI fluorescence and H&E staining. Additionally, we performed scanning electron microscopy (SEM), Pentachrome and Picrosirius-Red staining. Results showed that significantly higher DC depths are achieved on outer compared to inner surfaces (61 ± 7%; p < 0.001). Furthermore, the PD showed a high time dependency for all samples. Compared to continuous rocking, we achieved a significant increase in the DC efficiency through cyclic treatments ( ∼ 43%), whirling ( ∼ 19%) and sonication ( ∼ 49%). The combined treatment supported these results. In all procedures, a skeletonized but intact Collagen fibrous network was obtained as confirmed by SEM analysis. In conclusion, we systematically identified essential factors to significantly enhance DC procedures. We highly recommend considering these factors in future DC protocols. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 153-162, 2018.
Magnetic screening currents and coupling losses induced in superconducting magnets for thermonuclear fusion

Les tokamaks visent a produire de l'energie par fusion thermonucleaire en chauffant un plasma d'hydrogene jusqu'a 150 millions K et en le confinant a l’aide d’un champ magnetique intense cree par des aimants transportant d’importants courants. La supraconductivite est un atout precieux ici car permettant de reduire la taille des aimants et leur consommation energetique en contrepartie d’un refroidissement cryogenique. Cependant, dans les tokamaks, des variations de champ magnetique apparaissent (ex : decharge du solenoide central) et generent des pertes par induction dans les aimants. Si leur temperature augmente trop, ils peuvent perdre leur etat supraconducteur lors d’une transition brutale appelee "quench": afin de les proteger, ils sont decharges de leur courant entrainant ainsi la perte du plasma. Nous avons concentre notre travail sur la modelisation de ces pertes car leur connaissance est cruciale pour le bon dimensionnement du refroidissement des aimants et la prediction des limites operationnelles du tokamak. Afin d'ameliorer la comprehension physique de ce phenomene complexe et de proposer des solutions simples mais realistes, facilement integrables dans des plateformes multiphysiques deja fortement sollicitees par la modelisation d'autres effets, nous avons choisi d'adopter une approche analytique. Les câbles presents dans les tokamaks ayant une architecture assez complexe (centaines de brins torsades ensemble), nous avons mene des etudes analytiques et experimentales aux differentes echelles du câble; nous comparons ensuite les resultats de notre approche a ceux d'autres modeles existants (ex : numeriques) et, lorsque cela est possible, a l'experience.
C 174 T polymorphism in the CNTF receptor gene is associated with fat-free mass in men and women

Stephen M. Roth,1,2 E. Jeffrey Metter,3 Mechele R. Lee,1 Ben F. Hurley,2 and Robert E. Ferrell1 1Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261; 2Department of Kinesiology, University of Maryland, College Park, 20742; and 3Clinical Research Branch, National Institute on Aging, Gerontology Research Center, Baltimore, Maryland 21224
Competitive priorities of manufacturing firms in the Caribbean

This paper seeks to examine the competitive priorities emphasized by manufacturing firms in the Caribbean. We study five manufacturing competitive priorities - quality, flexibility, cost, delivery and innovation. Survey data from 60 manufacturing firms in 4 Caribbean countries show that cost and quality are most strongly emphasized while innovation is the least emphasized. The findings from our study also provide support for the cumulative capabilities theory and show that Caribbean manufacturers are simultaneously emphasizing all five priorities and do not appear to be trading off one priority for another. In light of these findings, the implications for manufacturing managers and policymakers in the Caribbean and other developing economies are discussed. Keywords: Caribbean; Competitive priority; Developing countries; Manufacturing strategy; Operations management
An Analysis of Shareholder Agreements

Shareholder agreements govern the relations among shareholders in privately held firms, such as joint ventures and venture capital-backed companies. We provide an economic explanation for key clauses in such agreements—namely, put and call options, tag-along and drag-along rights, demand and piggy-back rights, and catch-up clauses. In a dynamic moral hazard setting, we show that these clauses can ensure that the contract parties make efficient ex ante investments in the firm. They do so by constraining renegotiation. In the absence of the clauses, ex ante investment would be distorted by unconstrained renegotiation aimed at (i) precluding value-destroying ex post transfers, (ii) inducing value-increasing ex post investments, or (iii) precluding hold-out on value-increasing sales to a trade buyer or the IPO market.
Novel Potent Inhibitors of Deoxycytidine Kinase Identified and Compared by Multiple Assays

Deoxycytidine kinase (dCK) phosphorylates deoxycytidine, deoxyguanosine, and deoxyadenosine and plays an important role in the salvage pathway of nucleoside metabolism. dCK is also required for the phosphorylation of several antiviral and anticancer nucleoside drugs, with resistance to these agents often being associated with a loss or decrease in dCK activity. Data also indicate a role for dCK in immune function, and dCK inhibitors may provide treatment for immune disorders. To identify novel dCK inhibitors, the authors evaluated 2 existing biochemical assays, adapted both to high-throughput screening, and identified several series of hits. They also compared the potency of the hits between purified recombinant and endogenous enzyme. Meanwhile, they also developed a novel cell-based assay that rests on the rescue of cells from dCK-dependent cytotoxic agents such as AraC. A large number of compounds were tested using the 3 assays, and a strong correlation in potency was observed between the biochemical assay using endogenous enzyme and the cell-based assay. The hits identified in these screens have proved to be good starting points for the synthesis of much more potent tool compounds to further investigate the physiological functions of dCK and potentially lead to the development of therapeutic agents.
Quantitative identification of senescent cells in aging and disease

Senescent cells are present in premalignant lesions and sites of tissue damage and accumulate in tissues with age. In vivo identification, quantification and characterization of senescent cells are challenging tasks that limit our understanding of the role of senescent cells in diseases and aging. Here, we present a new way to precisely quantify and identify senescent cells in tissues on a single‐cell basis. The method combines a senescence‐associated beta‐galactosidase assay with staining of molecular markers for cellular senescence and of cellular identity. By utilizing technology that combines flow cytometry with high‐content image analysis, we were able to quantify senescent cells in tumors, fibrotic tissues, and tissues of aged mice. Our approach also yielded the finding that senescent cells in tissues of aged mice are larger than nonsenescent cells. Thus, this method provides a basis for quantitative assessment of senescent cells and it offers proof of principle for combination of different markers of senescence. It paves the way for screening of senescent cells for identification of new senescence biomarkers, genes that bypass senescence or senolytic compounds that eliminate senescent cells, thus enabling a deeper understanding of the senescent state in vivo.
Media use and Ethnolinguistic Vitality in bilingual communities

Abstract This article addresses the relationships between media, media use and language retention. In pursuing this aim, we explore the utility of ethnolinguistic vitality (EV) as a fruitful conceptual tool. The extant research on the relationship between the media and language retention and development provides an encouragement to pursue in more detail the role of media in this process: in other words to address more explicitly the features of media operation, and their relationship to audiences, which interface with the dynamics of EV. Based on four case studies of bilingual communities, the article concludes that media can be an important vehicle in maintaining and supporting EV. The extent to which this can be reached depends mostly on objective factors, such as the institutional completeness of the media landscape.