Miracle-dz/UniFAR-FTUs huggingface dataset:
Saturation Mutagenesis of Toluene ortho-Monooxygenase of Burkholderia cepacia G4 for Enhanced 1-Naphthol Synthesis and Chloroform Degradation

ABSTRACT Directed evolution of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 previously created the hydroxylase α-subunit (TomA3) V106A variant (TOM-Green) with increased activity for both trichloroethylene degradation (twofold enhancement) and naphthalene oxidation (six-times-higher activity). In the present study, saturation mutagenesis was performed at position A106 with Escherichia coli TG1/pBS(Kan)TOMV106A to improve TOM activity for both chloroform degradation and naphthalene oxidation. Whole cells expressing the A106E variant had two times better naphthalene-to-1-naphthol activity than the wild-type cells (Vmax of 9.3 versus 4.5 nmol · min−1 · mg of protein−1 and unchanged Km), and the regiospecificity of the A106E variant was unchanged, with 98% 1-naphthol formed, as was confirmed with high-pressure liquid chromatography. The A106E variant degrades its natural substrate toluene 63% faster than wild-type TOM does (2.12 ± 0.07 versus 1.30 ± 0.06 nmol · min−1 · mg of protein−1 [mean ± standard deviation]) at 91 μM and has a substantial decrease in regiospecificity, since o-cresol (50%), m-cresol (25%), and p-cresol (25%) are formed, in contrast to the 98% o-cresol formed by wild-type TOM. The A106E variant also has an elevated expression level compared to that of wild-type TOM, as evidenced by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Another variant, the A106F variant, has 2.8-times-better chloroform degradation activity based on gas chromatography (Vmax of 2.61 versus 0.95 nmol · min−1 · mg of protein−1 and unchanged Km) and chloride release (0.034 ± 0.002 versus 0.012 ± 0.001 nmol · min−1 · mg of protein−1). The A106F variant also was expressed at levels similar to those of wild-type TOM and 62%-better toluene oxidation activity than wild-type TOM (2.11 ± 0.3 versus 1.30 ± 0.06 nmol · min−1 · mg of protein−1). A shift in regiospecificity of toluene hydroxylation was also observed for the A106F variant, with o-cresol (28%), m-cresol (18%), and p-cresol (54%) being formed. Statistical analysis was used to estimate that 292 colonies must be screened for a 99% probability that all 64 codons were sampled during saturation mutagenesis.
A Load–Pull Characterization Technique Accounting for Harmonic Tuning

A novel methodology for the characterization of the nonlinear dynamic behavior of electron devices (EDs) is presented. It is based on a complete and accurate ED characterization that is provided by large-signal low-frequency I/V measurements, performed by means of a low-cost setup, in conjunction with any model-based description of the nonlinear reactive effects related to ED capacitances. The unique feature of the proposed technique is that a fully harmonic control of waveforms at the current generator plane is achieved, and as a consequence, high-efficiency operation can be simply investigated. Different experimental data are presented on GaAs and GaN transistors, and to definitely verify the capability of the new approach, the design of a class-F GaN power amplifier is deeply investigated as a case study.
Analytical Estimation of Map Readability

Readability is a major issue with all maps. In this study, we evaluated whether we can predict map readability using analytical measures, both single measures and composites of measures. A user test was conducted regarding the perceived readability of a number of test map samples. Evaluations were then performed to determine how well single measures and composites of measures could describe the map readability. The evaluation of single measures showed that the amount of information was most important, followed by the spatial distribution of information. The measures of object complexity and graphical resolution were not useful for explaining the map readability of our test data. The evaluations of composites of measures included three methods: threshold evaluation, multiple linear regression and support vector machine. We found that the use of composites of measures was better for describing map readability than single measures, but we could not identify any major differences in the results of the three composite methods. The results of this study can be used to recommend readability measures for triggering and controlling the map generalization process of online maps.
Markers of Oxidative Damage Are Not Elevated in Otherwise Healthy Individuals With the Metabolic Syndrome

OBJECTIVE The role of oxidative damage in the pathogenesis of metabolic syndrome is poorly understood. RESEARCH DESIGN AND METHODS A detailed cross-sectional study was performed to assess the relationship between lipid oxidation products, γ-glutamyltransferase, high-sensitivity C-reactive protein (hs-CRP), and phospholipase activities with respect to the metabolic status in a cohort of otherwise healthy individuals. RESULTS A total of 179 individuals (87 men and 92 women) aged 43 ± 14 years (mean ± SD) participated in this study. There were no differences in the levels of plasma F2-isoprostanes, hydroxyeicosatetraenoic acids, cholesterol oxidation products, and phospholipase activities in individuals with features of metabolic syndrome. In multivariate analyses, serum hs-CRP was a consistent independent predictor of metabolic syndrome. CONCLUSIONS Minimal changes were observed in multiple markers of oxidative damage in a well-characterized cohort of individuals with features of metabolic syndrome.
Web Miner: A Tool for Discovery of Usage Patterns From Web Data

-As there is a huge amount of data available online, the World Wide Web is a fertile area for data mining research. In recent years a various surveys have been performed on static data of web sites to perform web usage mining. This paper deals with the Web usage mining of a website which is hosted on IIS web server. Web usage mining is the area of data mining which deals with the discovery and analysis of usage patterns from Web data, specifically web logs, in order to perform improvements in web based applications. Web usage mining consists of three phases, pre-processing, pattern discovery, and pattern analysis. After the completion of these three phases the user can find the required usage patterns and use this information for the specific needs. The research is being performed on a log file using Log Parser.
A novel Noise estimation by histogram equalization and optimal filtering for robust speech enhancement

Generally, Speech enhancement aims to improve speech quality by using various algorithms. The objective of enhancement is improvement in overall perceptual quality of degraded speech signal using audio signal processing techniques. In earlier, there are so many algorithms proposed for speech enhancement. But they are not able to enhance the speech effectively by reducing the noise components. Recently a new mathematical algorithm called Empirical mode decomposition (EMDF) method was proposed. Though this algorithm enhances the speech effectively the time taken to process this enhancement is too high. Mainly this is because of the IMF evaluations for the complete speech samples. To overcome this issue this paper proposes a Histogram based speech enhancement technique. The histogram proposed in this work estimates the noise components contaminated with the clean speech samples, an optimal filtering is proposed to filter those estimated noise samples.
Joint Data Scheduling and FEC Coding for Multihomed Wireless Video Delivery

This paper studies the problem of mobile video delivery in heterogenous wireless networks from a server to multihomed device. Most existing works only consider delivering video streaming on single path which bandwidth is limited causing ultimate video transmission rate. To solve this live video streaming transmission bottleneck problem, we propose a novel solution named Joint Data Allocation and Fountain Coding (JDAFC) method that contain below characters: (1) path selection, (2) dynamic data allocation, and (3) fountain coding. We evaluate the performance of JDAFC by simulation experiments using Exata and JVSM and compare it with some reference solutions. Experimental results represent that JDAFC outperforms the competing solutions in improving the video peak signal-to-noise ratio as well as reducing the end-to-end delay.
Exploring Facial Asymmetry Using Optical Flow

Human faces are highly but not precisely bilaterally symmetrical. We present in this letter a measurement of facial asymmetry based on the optical flow field between a face image and its bilaterally mirrored counterpart. We revisit the problem of facial asymmetry quantification and confirm some conclusions in previous research by applying the proposed asymmetry measurement on a dataset containing 4000 subjects. Moreover, the proposed measurement also contains information on facial asymmetry compensation and thus can be used to facilitate various face processing tasks such as face image beautification, 3D face reconstruction and face recognition. Experimental results show the flexibility and effectiveness of our proposal.
A Ground-Based Method of Assessing Urban Forest Structure and Ecosystem Services

To properly manage urban forests, it is essential to have data on this important resource. An efficient means to obtain this information is to randomly sample urban areas. To help assess the urban forest structure (e.g., number of trees, species composition, tree sizes, health) and several functions (e.g., air pollution removal, carbon storage and sequestration), the Urban Forest Effects (UFORE) model was developed. Data collection variables and model methods are detailed and urban forest structure results are compared among 14 United States cities with average tree density ranging between 22.5 trees/ha (9.1 trees/ac) in Casper, Wyoming, U.S. to 275.8 trees/ha (111.6 trees/ac) in Atlanta, Georgia, U.S. Advantages and disadvantages of this ground-based method of assessing urban forest structure, functions, and values are discussed.
Can Skin Allograft Occasionally Act as a Permanent Coverage in Deep Burns? A Pilot Study

BACKGROUND Skin allograft is the gold standard of wound coverage in patients with extensive burns; however, it is considered as a temporary wound coverage and rejection of the skin allograft is considered inevitable. In our study, skin allograft as a permanent coverage in deep burns is evaluated. METHODS Skin allograft survival was assessed in 38 patients from March 2009 to March 2014, retrospectively. Because of the lack of tissue specimen from the skin donors, patients with long skin allograft survival in whom the gender of donor and recipient of allograft was the same were excluded. Seven cases with skin allograft longevity and opposite gender in donor and recipient were finally enrolled. A polymerase chain reaction (PCR) test on the biopsy specimen from recipients and donors were undertaken. RESULTS PCR on the biopsy specimen from recipients confirmed those specimens belong to the donors. All patients received allograft from the opposite sex. Two (28.57%) patients received allograft from their first-degree blood relatives, and in one (14.29%) case, the allograft was harvested from an alive individual with no blood relation. The rest were harvested from multiorgan donors. In eight months of follow up, no clinical evidence of graft rejection was noted. CONCLUSION Long term persistence of skin allograft in patients is worthy of more attention. Further studies An increase in knowledge of factors influencing this longevity could realize the dream of burn surgeons to achieve a permanent coverage other than autograft for major burn patients.
A TEST OF THE MONEY MULTIPLIER MODEL FOR A SMALL OPEN ECONOMY: THE CASE OF KUWAIT / TEST DU MODELE MULTIPLICATEUR POUR UNE ECONOMIE PETITE OUVERTE: LE CAS DU KOWEIT

The objective of this paper is to test the validity of the money multiplier model for small open economies using Kuwait as a case study. A battery of econometric tests reveals the absence of a long-run relationship between broad money and the monetary base. Although the necessary condition for such a relationship is satisfied in the case narrow money, the sufficient condition is not satisfied and the relationship is structurally unstable. It is concluded that the money multiplier model cannot serve as a framework for conducting monetary policy. TEST DU MODELE MULTIPLICATEUR POUR UNE ECONOMIE PETITE OUVERTE: LE CAS DU KOWEIT Resume L'objectifde cette etude estde mettre a I'epreuve la validite du modele multiplicateur monetaire pour les economies petites ouvertes en utilisant le Koweit comme cas d'etude. Un groupe d'essais econometriques revele /'absence d'une relation a long terme entre la monnaie large et la base monetaire. Bien que la condition necessaire soit satisfaite dans le cas de la monnaie etroite, la condition suffisante n'est pas satisfaite et la relation est structurellement instable. On conclutque le modele du multiplicateur monetaire ne pourra pas servir comme cadre ou on peut conduire une politique monetaire.
A Joint Time Synchronization and Localization Design for Mobile Underwater Sensor Networks

Time synchronization and localization are basic services in a sensor network system. Although they often depend on each other, they are usually tackled independently. In this work, we investigate the time synchronization and localization problems in underwater sensor networks, where more challenges are introduced because of the unique characteristics of the water environment. These challenges include long propagation delay and transmission delay, low bandwidth, energy constraint, mobility, etc. We propose a joint solution for localization and time synchronization, in which the stratification effect of underwater medium is considered, so that the bias in the range estimates caused by assuming sound waves travel in straight lines in water environments is compensated. By combining time synchronization and localization, the accuracy of both are improved jointly. Additionally, an advanced tracking algorithm interactive multiple model (IMM) is adopted to improve the accuracy of localization in the mobile case. Furthermore, by combining both services, the number of required exchanged messages is significantly reduced, which saves on energy consumption. Simulation results show that both services are improved and benefit from this scheme.
Context-Awareness in MobiLife

The IST project MobiLife (IST-2004-511607) is approaching Context-Awareness from the perspective of mobile applications and their enabling components. It envisions a highly distributed and loosely coupled solution in order to exchange context information on different semantic context levels between applications, enabling components and raw context sources. Semantic meaning of the context information exchanged is added via distributed ontology’s attached to it. The definition of the mechanism and interfaces is described by the MobiLife Context Management Framework. Actual context data used within MobiLife applications is provided and defined by a number of specific context providers. New context providers can be easily introduced based on the generic definition of them. The paper aims to provide the overview to the MobiLife context-awareness approach and describes its usage by the means of two MobiLife applications.
Experimental Study on Sentiment Classification of Chinese Review using Machine Learning Techniques

Machine learning method in text classification has expanded from topic identification to more challenging tasks such as sentiment classification, and it is valuable to explore, compare methods applied in sentiment classification and investigate relevant influence factors. The chief aim of the present work is to compare four machine learning methods to sentiment classification of Chinese review. The corpus is made up of 16000 reviews from website. We investigate the factors which affect the performance: namely feature representation via Word-Based Unigram (WBU), Bigram (WBB) and Chinese Character-Based Bigram (CBB), Trigram (CBT); feature weighting schemes and feature dimensionality. Experimental evaluations show that performance depends on different settings. As a result, we draw a conclusion that Naive Bayes (NB) classifier obtains the best averaging performance when using WBB, CBT as features with bool weighting under different dimensionality to the task.
THE SYSTEMATIC NATURE OF SERENDIPITY IN OPPORTUNITY DISCOVERY: IMPLICATIONS FOR THE THEORY OF ENTREPRENEURSHIP (SUMMARY)

In this paper, we explore the systematic nature of opportunity discovery using a goal-setting framework. With an experiment, we demonstrate that the discovery of non-obvious solutions, their effectiveness, and the search processes associated with the discovery are affected by the nature of assigned goals. We discuss these results and offer some insights into the mechanism associated with the serendipitous discovery of opportunities.
Palestine: a theoretical model of an Investment-Constrained Economy

The sixty-year-old Israeli-Palestinian conflict has deeply influenced the evolution of the Palestinian economy. In the last two decades persisting political instability and the Israeli closure policy have been sources of protracted economic stagnation and poor capital formation. The paper describes the consequences on the Palestinian economy of two particular conditions: high transaction costs and market fragmentation. We use a simple one-sector model which describes Palestine as a demand-driven economy and Palestinian capital accumulation as linked to desired investments by Palestinian firms. Into this framework, we show that high transaction costs discourage capital formation by curtailing expected profitability. Market fragmentation further reduces domestic investments by reducing the size of the market and depressing entrepreneurs’ animal spirits. We show that in the short-run, where expectations are given, the two above facts induce low levels of capacity utilization and of capital accumulation. The situation is even more worrying in the long-run when entrepreneurs can adapt their expectations. Depressed animal spirits and low levels of capacity use feed back into each other and give rise to a low-growth trap from which Palestine can hardly escape. We also highlight the possible positive impact of the removal of high transaction cost and of market fragmentation and the ensuing benefits on the long run equilibrium values of both capital accumulation and capacity utilization. The conclusions try to set this analytical results into the historical situation of the Palestinian economy and to envisage the roles of economics and politics in order to establish a sustained process of development.
Towards an efficient and robust foot classification from pedobarographic images

This paper presents a new computational framework for automatic foot classification from digital plantar pressure images. It classifies the foot as left or right and simultaneously calculates two well-known footprint indices: the Cavanagh's arch index (AI) and the modified AI. The accuracy of the framework was evaluated using a set of plantar pressure images from two common pedobarographic devices. The results were outstanding, as all feet under analysis were correctly classified as left or right and no significant differences were observed between the footprint indices calculated using the computational solution and the traditional manual method. The robustness of the proposed framework to arbitrary foot orientations and to the acquisition device was also tested and confirmed.
The robot localization problem in two dimensions

We consider the following problem: given a simple polygon <italic>P</italic> and a star-shaped polygon <italic>V</italic>, find a point (or the set of points) in <italic>P</italic> from which the portion of <italic>P</italic> that is visible is congruent to <italic>V</italic>. The problem arises in the localization of robots using a range-finder—<italic>P</italic> is a map of a known environment, <italic>V</italic> is the portion visible from the robot's position, and the robot must use this information to determine its position in the map. We give a scheme that preprocesses <italic>P</italic> so that any subsequent query <italic>V</italic> is answered in optimal time <italic>O</italic>(<italic>m</italic> + log <italic>n</italic> + A), where <italic>m</italic> and <italic>n</italic> are the number of vertices in <italic>V</italic> and <italic>P</italic>, and A is the number of points in <italic>P</italic> that are valid answers (the output size). Our technique allows us to trade off smoothly between the query time and the preprocessing time or space. We also devise a data structure for output-sensitive determination of the visibility polygon of a query point inside a polygon <italic>P</italic>. We then consider a variant of the localization problem in which there is a maximum distance to which the robot can “see”—this is motivated by practical considerations, and we outline a similar solution for this case. We also show that a single localization query <italic>V</italic> can be answered in time <italic>O</italic>(<italic>mn</italic>) with no preprocessing.
Report Documentation Page Form Approved Study of Compression-loaded and Impact-damaged Structurally Ecient Graphite-thermoplastic Trapezoidal-corrugation Sand- Wich and Semisandwich Panel

Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden,
The Use of Orthogonal Frequency Code Division (OFCD) Multiplexing in Wireless Mesh Network (WMN)

In the present scenario, improvement in the data rate, network capacity, scalability, and the network throughput are some of the most serious issues in wireless mesh networks (WMN). Specifically, a major obstacle that hinders the widespread adoption of WMN is the severe limits on throughput and the network capacity. This chapter presents a discussion on the potential use of a combined orthogonal-frequency code-division (OFCD) multiple access scheme in a WMN. The OFCD is the combination of orthogonal frequency division multiplexing (OFDM) and the code division multiple access (CDMA). Since ODFM is one of the popular multi-access schemes that provide high data rates, combing the OFDM with the CDMA may yield a significant improvement in a WMN in terms of a comparatively high network throughput with the least error ration. However, these benefits demand for more sophisticated design of transmitter and receiver for WMN that can use OFCD as an underlying multiple access scheme. In order to demonstrate the potential use of OFCD scheme with the WMN, this chapter presents a new transmitter and receiver model along with a comprehensive discussion on the performance of WMN under the new OFCD multiple access scheme. The purpose of this analysis and experimental verification is to observe the performance of new transceiver with the OFCD scheme in WMN with respect to the overall network throughput, bit error rate (BER) performance, and network capacity. Moreover, in this chapter, we provide an analysis and comparison of different multiple access schemes such as FDMA, TDMA, CDMA, OFDM, and the new OFCD. Keywords— Wireless mesh networks, orthogonal frequency code division (OFCD), Code division multiple access (CDMA), Multiple access, Multiple access interference (MAI), Network throughput, Bit error rate (BER), Intersymbol interference (ISI).