Miracle-dz/UniFAR-FTUs huggingface dataset:
Body Sensor Networks: In the Era of Big Data and Beyond

Body sensor networks (BSN) have emerged as an active field of research to connect and operate sensors within, on or at close proximity to the human body. BSN have unique roles in health applications, particularly to support real-time decision making and therapeutic treatments. Nevertheless, challenges remain in designing BSN nodes with antennas that operate efficiently around, ingested or implanted inside the human body, as well as new methods to process the heterogeneous and growing amount of data on-node and in a distributed system for optimized performance and power consumption. As the battery operating time and sensor size are two important factors in determining the usability of BSN nodes, ultralow power transceivers, energy-aware network protocol, data compression, on-node processing, and energy-harvesting techniques are highly demanded to ultimately achieve a self-powered BSN.
Ex vivo cultures combined with vivo-morpholino induced gene knockdown provide a system to assess the role of WT1 and GATA4 during gonad differentiation

Gonad morphogenesis relies on the correct spatiotemporal expression of a number of genes that together fulfill the differentiation of the bipotential gonad into testes or ovaries. As such, the transcription factors WT1 and GATA4 are pivotal for proper gonadal development. Here we address the contributions of GATA4 and WT1 to the sex differentiation phase in testes and ovaries. We applied an ex vivo technique for cultivating gonads in hanging droplets of media that were supplemented with vivo-morpholinos to knockdown WT1 and GATA4 either alone or in combination at the same developmental stage. We show that WT1 is equally important for both, the initial establishment and the maintenance of the sex-specific gene expression signature in testes and ovaries. We further identified Foxl2 as a novel putative downstream target gene of WT1. Moreover, knockdown of WT1 reduced mRNA levels of several molecular components of the hedgehog signaling pathway in XY gonads, whereas Gata4 vivo-morpholino treatment increased transcripts of Dhh and Ptch1 in embryonic testes. The data suggest that for its proper function, WT1 relies on the correct expression of the GATA4 protein. Furthermore, GATA4 down-regulates several ovarian promoting genes in testes, such as Ctnnb1, Fst, and Bmp2, suggesting that this repression is required for maintaining the male phenotype. In conclusion, this study provides novel insights into the role of WT1 and GATA4 during the sex differentiation phase and represents an approach that can be applied to assess other proteins with as yet unknown functions during gonadal development.
Isometric Torque Generation in a Parkinsonian tremulous elbow and the effect of medication

Individuals afflicted with Parkinson's disease (PD) have impaired force or torque control. It has also been reported that force or torque production, either dynamic or isometric, may be debilitated by PD in certain muscles and joints. During isometric contractions, the reported impairments were mostly in the rate of force changes, in latency, and in Maximum Voluntary Contraction (MVC) levels. However, in tremor dominant PD patients, it is of interest to investigate the effect of medication on the overall intended torque generation, with the tremor (unwanted) component being averaged out. This study examined average (tremor removed) isometric torque generation in submaximal contractions of elbow flexion-extension. The patient in this case study had unusually strong, but dopa-responsive, rest and isometric tremors. Ridge regression models were used to relate the average generated torque to average EMG in four main elbow flexor and extensor muscles. If changes in MVC were the result of medication, a reasonable assumption would be a mere scaling in the force production model. However, the results suggest that, in both tremor-dominant and non-tremor-dominant hands, the model parameters were different for the on-medication state compared to the off-medication state. This finding may indicate that relative activation of each muscle involved in this motion is affected by anti-Parkinsonian medication.
Development of the Tinnitus Handicap Inventory.

OBJECTIVE To develop a self-report tinnitus handicap measure that is brief, easy to administer and interpret, broad in scope, and psychometrically robust. DESIGN A standardization study of a self-report tinnitus handicap measure was conducted to determine its internal consistency reliability and convergent and construct validity. SETTING Audiology clinics in tertiary care centers in two sites. PARTICIPANTS In the first investigation, 84 patients reporting tinnitus as their primary complaint or secondary to hearing loss completed the 45-item alpha version of the Tinnitus Handicap Inventory (THI). In the second investigation, 66 subjects also reporting tinnitus completed the 25-item beta version. OUTCOME MEASURES Convergent validity was assessed using another measure of perceived tinnitus handicap (Tinnitus Handicap Questionnaire). Construct validity was assessed using the Beck Depression Inventory, Modified Somatic Perception Questionnaire, symptom rating scales (annoyance, sleep disruption, depression, and concentration), and perceived tinnitus pitch and loudness judgments. RESULTS From the alpha version of the THI, we derived a 25-item beta version with the items grouped into functional, emotional, and catastrophic subscales. The total scale yielded excellent internal consistency reliability (Cronbach's alpha = .93). No significant age or gender effects were seen. Weak correlations were observed between the THI and the Beck Depression Inventory, Modified Somatic Perception Questionnaire, and pitch and loudness judgments. Significant correlations were found between the THI and the symptom rating scales. CONCLUSION The THI is a self-report measure that can be used in a busy clinical practice to quantify the impact of tinnitus on daily living.
Vanadate-resistant mutants of Saccharomyces cerevisiae show alterations in protein phosphorylation and growth control

This work describes two spontaneous vanadate-resistant mutants of Saccharomyces cerevisiae with constitutive alterations in protein phosphorylation, growth control, and sporulation. Vanadate has been shown by a number of studies to be an efficient competitor of phosphate in biochemical reactions, especially those that involve phosphoproteins as intermediates or substrates. Resistance to toxic concentrations of vanadate can arise in S. cerevisiae by both recessive and dominant spontaneous mutations in a large number of loci. Mutations in two of the recessive loci, van1-18 and van2-93, resulted in alterations in the phosphorylation of a number of proteins. The mutant van1-18 gene also showed an increase in plasma membrane ATPase activity in vitro and a lowered basal phosphatase activity under alkaline conditions. Cells containing the van2-93 mutant allele had normal levels of plasma membrane ATPase activity, but this activity was not inhibited by vanadate. Both of these mutants failed to enter stationary phase, were heat shock sensitive, showed lowered long-term viability, and sporulated on rich medium in the presence of 2% glucose. The wild-type VAN1 gene was isolated and sequenced. The open reading frame predicts a protein of 522 amino acids, with no significant homology to any genes that have been identified. Diploid cells that contained two mutant alleles of this gene demonstrated defects in spore viability. These data suggest that the VAN1 gene product is involved in regulation of the phosphorylation of a number of proteins, some of which appear to be important in cell growth control.
Evaluation and Measurement of the Internal Performance of the Civil Regime of the Moroccan Pension Fund

The civil pension scheme of the Moroccan pension fund is â€˜Bismarkianâ€™, contributory and mandatory. This pay-as-you-go system finds its foundation in social justice and intergenerational equity. The evaluation of the sustainability and viability of this scheme can not be carried out solely by the financial component, even if this criterion is essential to ensure the confidence of the affiliates. It also requires consideration of other criteria to ensure its ability to meet its commitments and achieve its objectives. This paper proposes to analyze and evaluate the internal civil regime performance of the Moroccan Pension Fund (CMR) based on the identification and measurement of indicators of three main parameters: generosity, equity, and the inter and intra generational redistribution.
Targeted genetics in Drosophila cell lines: Inserting single transgenes in vitro

ABSTRACT A long-standing problem with analyzing transgene expression in tissue-culture cells is the variation caused by random integration of different copy numbers of transfected transgenes. In mammalian cells, single transgenes can be inserted by homologous recombination but this process is inefficient in Drosophila cells. To tackle this problem, our group, and the Cherbas group, used recombination-mediated cassette exchange (RMCE) to introduce single-copy transgenes into specific locations in the Drosophila genome. In both cases, ϕC31 was used to catalyze recombination between its target sequences attP in the genome, and attB flanking the donor sequence. We generated cell lines de novo with a single attP-flanked cassette for recombination, whereas, Cherbas et al. introduced a single attP-flanked cassette into existing cell lines. In both approaches, a 2-drug selection scheme was used to select for cells with a single copy of the donor sequence inserted by RMCE and against cells with random integration of multiple copies. Here we describe the general advantages of using RMCE to introduce genes into fly cells, the different attributes of the 2 methods, and how future work could make use of other recombinases and CRISPR/Cas9 genome editing to further enable genetic manipulation of Drosophila cells in vitro.
195-nW 120-dB subthreshold CMOS OTA driving up to 200 pF and occupying only 4.4–10−3 mm2

An ultra-low-power high-performance three-stage subthreshold CMOS OTA is presented together with its detailed design methodology. The amplifier is realized in a 0.35-μm technology, is powered from a 1-V supply, exhibits 120-dB DC gain and is designed to drive a capacitive load up to 200 pF. Despite the allowed heavy load, it occupies only 4.4-10-3 mm2 and dissipates only 195 nW at DC, while providing 20-kHz unity gain bandwidth. Comparison with prior art shows an improvement in the figures of merit by a factor greater than 5.
Herpes simplex virus 1 reiterated S component sequences (c1) situated between the a sequence and alpha 4 gene are not essential for virus replication

The herpes simplex virus 1 genome consists of two components, L and S, each containing unique sequences flanked by inverted repeats. Each of the 6.5-kilobase pair inverted repeats of the S component, designated a'c' and ca, contains an approximately 700-base pair sequence (designated c1) located between the a sequence and the 3' terminus of the alpha 4 gene. Like the a sequence, c1 consists of direct repeats and unique sequences. Its function is not known. To probe for its function, we constructed a plasmid containing a viral thymidine kinase (TK) gene inserted into the c1 sequence. The construct was recombined into the genome of a TK- virus by cotransfection with intact viral DNA and selection for TK+ virus. As predicted from previous studies (Knipe et al., Proc. Natl. Acad. Sci. U.S.A. 75:3896-3900, 1978), the TK gene was found to be present in both copies of the c1 sequence in the R3104 virus. To delete the c1 sequence we constructed a plasmid containing 4 kilobase pairs of pBR322 flanked by an a sequence and by structural sequences of the alpha 4 gene. In this instance the cells were transfected with the construct and R3104 DNA; the progeny of the transfection was plated in the presence of 5-bromo-2'-deoxyuridine, and the selection was for TK- virus (R3158). The pBR322 DNA sequences replaced the c1 at both termini of the S component in R3158 DNA, but a sequence homologous to c1 was present in proximity to the 3' terminus of the alpha 4 gene. The results indicate that the c1 region has no significant role in the replication of the virus in cell culture. The advantage of inserting the pBR322 sequence is that it permits efficient cloning of large herpes simplex virus 1 DNA fragments by simple ligation of digests and transformation of appropriate Escherichia coli strains. The effortless selection of recombinants carrying inserts in both copies of the c1 restates the usefulness of this technique for selection of insertion deletion recombinants and underscores the rapid emergence of sequence identity at both ends of the reiterated regions of the S component as previously reported (Knipe et al., Proc. Natl. Acad. Sci. U.S.A. 75:3896-3900, 1978).
DPSCs from Inflamed Pulp Modulate Macrophage Function via the TNF-α/IDO Axis

Human dental pulp stem cells (DPSCs) can be isolated from inflamed pulp derived from carious teeth with symptomatic irreversible pulpitis (I-DPSCs), which possess stemness and multidifferentiation potentials similar to DPSCs from healthy pulp. Since macrophages—essential cell players of the pulpal innate immunity—can regulate pulpal inflammation and repair, the authors investigated the immunomodulatory effects of DPSCs/I-DPSCs on macrophage functions and their underlying mechanisms. Similar to DPSCs, I-DPSCs were capable of colony-forming efficiency and adipogenic and osteo/dentinogenic differentiation under in vitro induction conditions. I-DPSCs also expressed a similar phenotypic profile of mesenchymal stem cell markers, except a relatively higher level of CD146 as compared with DPSCs. Coculture of DPSCs or I-DPSCs with differentiated THP-1 cells, the human monocyte cell line, markedly suppressed tumor necrosis factor α (TNF-α) secretion in response to stimulation with lipopolysaccharides (LPS) and/or nigericin. However, unlike TNF-α, the secreted level of interleukin 1β was not affected by coculture with DPSCs or I-DPSCs. Furthermore, DPSC/I-DPSC-mediated inhibition of TNF-α secretion by macrophages was abolished by pretreatment with 1-methyl-D-tryptophan, a specific inhibitor of indoleamine-pyrrole 2,3-dioxygenase (IDO), but not by NSC-398, a specific inhibitor of COX-2, suggesting IDO as a mediator. Interestingly, IDO expression was significantly augmented in macrophages and mesenchymal stromal cells in inflamed human pulp tissues. Collectively, these findings show that I-DPSCs, similar to DPSCs, possess stem cell properties and suppress macrophage functions via the TNF-α/IDO axis, thereby providing a physiologically relevant context for their innate immunomodulatory activity in the dental pulp and their capability for pulp repair.
Cost analysis of rice milling: a case study of 7 rice mills in Malaysia

Purpose - The purpose of this paper is to examine the long-run profitability of rice milling operation in Malaysia and see how sensitive it is to changes in paddy purchases, rice recovery ratio and paddy price. Design/methodology/approach - Using a purposive sampling technique and semi-structured interviews, seven rice mills in Malaysia were selected to obtain data on operational details and business accounts. The paper provides a qualitative and descriptive account of the rice mill’s profitability by using cost curves, a simple linear regression and the Monte Carlo simulation. Findings - The rice milling operation in Malaysia is profitable in the long run, provided that there is a market for by-products. Large private mills have lower average costs, helping them obtain higher profit margins. Public mills that receive a rice miller subsidy are more protected than small private mills that operate without the rice miller subsidy and under price controls. Changes in paddy purchases, paddy price and recovery ratio affect the profitability to varying degrees. Research limitations/implications - Incomplete information provided by the interviewees. The analysis result is only a rough estimate. However, it may provide a useful insight into the Malaysian rice milling sector and its competitiveness. Originality/value - There are few economic studies of the rice milling sector in Malaysia. However, it plays an important role along the rice supply chain. There is a need for better understanding of the industry in order to obtain economic sustainability and effective policies. This paper provides the gap by providing an insight into the long-run profitability of rice milling operation in Malaysia.
Iberian Neolithic Networks: The Rise and Fall of the Cardial World

Recent approaches have described the evolutionary dynamics of the first Neolithic societies as a cycle of rise and fall. Several authors, using mainly c14 dates as a demographic proxy, identified a general pattern of a boom in population coincident with the arrival of food production economies followed by a rapid decline some centuries afterwards in multiple European regions. Concerning Iberia, we also noted that this phenomenon correlates with an initial development of archaeological entities (i.e., ‘cultures’) over large areas (e.g. the Impresso-Cardial in West Mediterranean), followed by a phase of ‘cultural fragmentation’ by the end of Early Neolithic. These results in a picture of higher cultural diversity as an effect of more limited spread of cultural artifacts. In this work we propose to apply a network approach to the analysis of material culture. In particular, we consider the spatiotemporal patterns of material culture as an emergent effect of interaction processes acting locally. As recent research has pointed out, the spatiotemporal variability of material culture is an emergent phenomena resulting of individual and group interactions whose structure resembles those of spatially-structured complex Networks. Our results suggest that the observed global patterns could be explained by the network dynamics, specially by structural (measured as the Betweenness Centrality) and geographical position of some nodes. The appearance and disappearance of nodes in specific positions correlates with the observed changes in the pattern of material culture distribution throughout the Early Neolithic (c. 7700-6700 cal BP) in East Iberia. In our view, this could be explained by the especial role played by those nodes facilitating or limiting the information flow over the entire network. Network growth and posterior fragmentation seem to be the key drivers behind these dynamics.
Surface potential modeling and reconstruction in Kelvin probe force microscopy

Kelvin probe force microscopy (KPFM) measurement has been extensively applied in metallic, semiconductor and organic electronic or photovoltaic devices, to characterize the local contact potential difference or surface potential of the samples at the nanoscale. Here, a comprehensive modeling of surface potential in KPFM is established, from the well-known single capacitance model to a precise electrodynamic model, considering the long range property of the electrostatic force in KPFM. The limitations and relations of different models are also discussed. Besides, the feedback condition of the KPFM system is reconsidered and modified, showing that the influence of the cantilever has been overestimated by about 20% in previous reports. Afterwards, the surface potential of charged Si-nanocrystals is reconstructed based on the electrodynamic model, and the calculated surface charge density is very consistent with the macroscopic capacitance–voltage (C–V) measurement. A deep understanding and correct reconstruction of surface potential is crucial to the quantitative analysis of KPFM results.
Fault detection with network communication

This article investigates the problem of fault detection for continuous time systems with network communication links. A network communication channel is assumed to be existing between the plant and the fault detection filter, and three types of incomplete measurements which typically appear in a network environment are simultaneously addressed, including measurement quantisation effect, signal transmission delay and data packet dropout. A mathematical model is presented to account for those issues in a unified form. Based on that, a full-order fault detection filter is designed such that the residual system is asymptotically stable and preserves a guaranteed performance. A sufficient condition of the existence of the fault detection filter is obtained and all the results are formulated in the form of linear matrix inequalities, which can be readily solved via standard numerical software. Finally, a simulation example is provided to illustrate the usefulness of the developed theoretical results.
A dramatic conformational rearrangement is necessary for the activation of DNR from Pseudomonas aeruginosa. Crystal structure of wild‐type DNR

The opportunistic pathogen Pseudomonas aeruginosa can grow in low oxygen, because it is capable of anaerobic respiration using nitrate as a terminal electron acceptor (denitrification). An intermediate of the denitrification pathway is nitric oxide, a compound that may become cytotoxic at high concentration. The intracellular levels of nitric oxide are tightly controlled by regulating the expression of the enzymes responsible for its synthesis and degradation (nitrite and nitric oxide reductases). In this article, we present the crystallographic structure of the wild‐type dissimilative nitrate respiration regulator (DNR), a master regulator controlling expression of the denitrification machinery and a putative target for new therapeutic strategies. Comparison with other structures among the CRP‐FNR class of regulators reveals that DNR has crystallized in a conformation that has never been observed before. In particular, the sensing domain of DNR has undergone a rotation of more than 50° with respect to the other structures. This suggests that DNR may undergo an unexpected and very large conformational rearrangement on activation. Proteins 2009. © 2009 Wiley‐Liss, Inc.
Food and Agricultural Industries: Opportunities for Improving Measurement and Reporting

We measure one component of off-farm food and agricultural industries using establishment level microdata in the federal statistical system. We focus on services for crop production, and compare measures of firm and employment dynamics in this sector during the period 1992-2012 with county-level publicly available data for the same measures. Based on differences across data sources, we establish new facts regarding the evolution of food and agricultural industries, and demonstrate the value of working with confidential microdata. In addition to the data and results we present, we highlight possibilities for collaboration across universities and federal agencies to improve reporting in other segments of food and agricultural industries.