Miracle-dz/UniFAR-FTUs huggingface dataset:
Neuroendocrine dysfunction in patients recovering from subarachnoid hemorrhage

OBJECTIVE: Subarachnoid hemorrhage (SAH) is a recently identified risk factor for hypopituitarism, particularly growth hormone (GH) and corticotrophins deficiencies. The aim of our study was to identify possible predictor(s) for neuroendocrine dysfunction in SAH survivors. DESIGN: Pituitary function was evaluated in 93 patients (30 males, 63 females), aged 48.0±1.1 years (mean±SE), and with a Glasgow Outcome Scale score of 4.6±0.6 (mean±SE) more than one year following SAH. In the acute phase, SAH was complicated by vasospasm (VS) in 18 and by hydrocephalus (HDC) in 9 patients. Baseline serum values of Insulin Growth Factor 1 (IGF-I), cortisol, Thyroxine (T4), Thyroid Stimulating Hormone (TSH), Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), testosterone (in males), estradiol (in females) and prolactin were determined. RESULTS: According to the results of baseline hormonal evaluation, 47 patients (50.5%) had no hormonal abnormalities. Seven patients (7.5%) had multiple pituitary hormone deficiencies: Four patients (4.3%) had two (GH and cortisol), one patient had three (gonadal, adrenal and GH) and two patients had deficiency of all pituitary axes. Thirty-nine patients (42%) had one abnormal axis (13 adrenal, 2 thyroid, 4 gonadal and 20 GH). None of the subjects was treated with desmopressin or exhibited symptomatic polyuria. The VS and HDC during the acute phase of SAH were related to abnormal pituitary status (VS with low IGF-I levels and HDC with low cortisol levels). CONCLUSION: Through a screening procedure, neuroendocrine dysfunction was identified in a substantial number of asymptomatic patients with previous SAH. Cerebral VS and HDC at the time of SAH emerged as risk factors possibly predicting development of pituitary dysfunction. Low basal levels of IGF 1 and cortisol may help in selecting patients requiring further evaluation of pituitary function.
Symmetry-Factored Statistical Modelling of Craniofacial Shape

We present a new method for symmetry-factored statistical modelling of 3D shape. Our method comprises three novel components. First, a means to symmetrise a 3D mesh, regularised using the Laplace-Beltrami operator. Second, a symmetry-aware variant of Generalized Procrustes Analysis (GPA). Third, a means to compute a linear statistical shape model in which symmetry and asymmetric shape variation are modelled separately. We focus on human head data and build the first 3D morphable model of craniofacial asymmetry. The qualitative and quantitative evaluation demonstrates that the proposed model outperforms a linear model that does not decompose symmetric and asymmetric variation. It also validates that symmetry-aware GPA can improve the data generalisation and reconstruction ability of the standard PCA model. We will make our model and the implementation of our method publicly available1.
Quantifying Biochemical Alterations in Brown and Subcutaneous White Adipose Tissues of Mice Using Fourier Transform Infrared Widefield Imaging

Stimulating increased thermogenic activity in adipose tissue is an important biological target for obesity treatment, and label-free imaging techniques with the potential to quantify stimulation-associated biochemical changes to the adipose tissue are highly sought after. In this study, we used spatially resolved Fourier transform infrared (FTIR) imaging to quantify biochemical changes caused by cold exposure in the brown and subcutaneous white adipose tissues (BAT and s-WAT) of 6 week-old C57BL6 mice exposed to 30°C (N = 5), 24°C (N = 5), and 10°C (N = 5) conditions for 10 days. Fat exposed to colder temperatures demonstrated greater thermogenic activity as indicated by increased messenger RNA expression levels of a panel of thermogenic marker genes including uncoupling protein 1 (UCP-1) and Dio2. Protein to lipid ratio, calculated from the ratio of the integrated area from 1,600 to 1,700 cm−1 (amide I) to the integrated area from 2,830 to 2,980 cm−1 (saturated lipids), was elevated in 10°C BAT and s-WAT compared to 24°C (p = 0.004 and p < 0.0001) and 30°C (p = 0.0033 and p < 0.0001). Greater protein to lipid ratio was associated with greater UCP-1 expression level in the BAT (p = 0.021) and s-WAT (p = 0.032) and greater Dio2 expression in s-WAT (p = 0.033). The degree of unsaturation, calculated from the ratio of the integrated area from 2,992 to 3,020 cm−1 (unsaturated lipids) to the integrated area from 2,830 to 2,980 cm−1 (saturated lipids), showed stepwise decreases going from colder-exposed to warmer-exposed BAT. Complementary 1H NMR measurements confirmed the findings from this ratio in BAT. Principal component analysis applied to FTIR spectra revealed pronounced differences in overall spectral characteristics between 30, 24, and 10°C BAT and s-WAT. Spatially resolved FTIR imaging is a promising technique to quantify cold-induced biochemical changes in BAT and s-WAT in a label-free manner.
Interplay of diffusion and phase transformations in microstructural evolution of the interaction zone between U–9 wt.% Mo and Zr–1 wt.% Nb alloys

Metallurgical interaction between U–9 wt. % Mo metallic fuel alloy and Zr–1 wt. % Nb clad material has been assessed. Interdiffusion of constituent elements across their interface, together with the phase reactions occurring at high temperature and during subsequent cooling, resulted in development of a layered interaction zone where coexistence of a bcc solid solution phase with varying compositions, along with α-U, α-Zr and Mo2Zr phases could be noticed.
Contaminación atmosférica en Dock Sud, pcia. de Buenos Aires, Argentina

Resumen. El Polo Petroquímico de Dock Sud es considerado un ícono de la contaminación en el Riachuelo. Desde finales del año 2010, se lleva adelante un monitoreo de la calidad de aire. El objetivo de este trabajo es caracterizar el comportamiento temporal de los contaminantes atmosféricos (NO, NO2, NOx, O3, CO, COVs, SO2, PM10 y PM2.5) medidos en el área de Dock Sud, Pcia. de Buenos Aires. Para ello se utilizó información de la estación de monitoreo continuo y automático EMC I Dock Sud, provista por ACUMAR, del período comprendido entre enero de 2011 y febrero de 2017. Se elaboró un análisis temporal de los contaminantes, que incluyó el estudio de las concentraciones en función de distintas variables ambientales y su comparación con normativas vigentes. PM 10, PM2.5, O3 y SO2 sobrepasaron los niveles guía de OMS en múltiples oportunidades al cabo de un año.
SHARPIN regulates collagen architecture and ductal outgrowth in the developing mouse mammary gland

SHARPIN is a widely expressed multifunctional protein implicated in cancer, inflammation, linear ubiquitination and integrin activity inhibition; however, its contribution to epithelial homeostasis remains poorly understood. Here, we examined the role of SHARPIN in mammary gland development, a process strongly regulated by epithelial–stromal interactions. Mice lacking SHARPIN expression in all cells (Sharpincpdm), and mice with a stromal (S100a4‐Cre) deletion of Sharpin, have reduced mammary ductal outgrowth during puberty. In contrast, Sharpincpdm mammary epithelial cells transplanted in vivo into wild‐type stroma, fully repopulate the mammary gland fat pad, undergo unperturbed ductal outgrowth and terminal differentiation. Thus, SHARPIN is required in mammary gland stroma during development. Accordingly, stroma adjacent to invading mammary ducts of Sharpincpdm mice displayed reduced collagen arrangement and extracellular matrix (ECM) stiffness. Moreover, Sharpincpdm mammary gland stromal fibroblasts demonstrated defects in collagen fibre assembly, collagen contraction and degradation in vitro. Together, these data imply that SHARPIN regulates the normal invasive mammary gland branching morphogenesis in an epithelial cell extrinsic manner by controlling the organisation of the stromal ECM.
Social Impact of Recharging Activity in Long-Term HRI and Verbal Strategies to Manage User Expectations During Recharge

Social robots perform tasks to help humans in their daily activities. However, if they fail to fulfill expectations this may affect their acceptance. This work investigates the service degradation caused by recharging, during which the robot is socially inactive. We describe two studies conducted in an ecologically valid office environment. In the first long-term study (3 weeks), we investigated the service degradation caused by the recharging behavior of a social robot. In the second study, we explored the social strategies used to manage users’ expectations during recharge. Our findings suggest that the use of verbal strategies (transparency, apology, and politeness) can make robots more acceptable to users during recharge.
Access to care for children with emotional/behavioral difficulties

Emotional/behavioral difficulties (EBDs) are increasingly diagnosed in children, constituting some of the most common chronic childhood conditions. Left untreated, EBDs pose long-term individual and population-level consequences. There is a growing evidence of disparities in EBD prevalence by various demographic characteristics. This article builds on this research by examining disparities in access to medical care for children with EBD. From 2008 to 2011, using data from the US National Health Interview Survey (N = 31,631) on sample children aged 4–17, we investigate (1) whether having EBD affects access to care (modeled as delayed care due to cost and difficulty making an appointment) and (2) the role demographic characteristics, health insurance coverage, and frequency of service use play in access to care for children with EBD. Results indicate that children with EBD experience issues in accessing care at more than twice the rate of children without EBD, even though they are less likely to be uninsured than their counterparts without EBD. In multivariable models, children with EBD are still more likely to experience delayed care due to cost and difficulty making a timely appointment, even after adjusting for frequency of health service use, insurance coverage, and demographic characteristics.
Anaplastic lymphoma kinase (ALK) gene rearrangements in radiation‐related human papillary thyroid carcinoma after the Chernobyl accident

Childhood radiation exposure has been associated with increased papillary thyroid carcinoma (PTC) risk. The role of anaplastic lymphoma kinase (ALK) gene rearrangements in radiation‐related PTC remains unclear, but STRN‐ALK fusions have recently been detected in PTCs from radiation exposed persons after Chernobyl using targeted next‐generation sequencing and RNA‐seq. We investigated ALK and RET gene rearrangements as well as known driver point mutations in PTC tumours from 77 radiation‐exposed patients (mean age at surgery 22.4 years) and PTC tumours from 19 non‐exposed individuals after the Chernobyl accident. ALK rearrangements were detected by fluorescence in situ hybridisation (FISH) and confirmed with immunohistochemistry (IHC); point mutations in the BRAF and RAS genes were detected by DNA pyrosequencing. Among the 77 tumours from exposed persons, we identified 7 ALK rearrangements and none in the unexposed group. When combining ALK and RET rearrangements, we found 24 in the exposed (31.2%) compared to two (10.5%) in the unexposed group. Odds ratios increased significantly in a dose‐dependent manner up to 6.2 (95%CI: 1.1, 34.7; p = 0.039) at Iodine‐131 thyroid doses >500 mGy. In total, 27 cases carried point mutations of BRAF or RAS genes, yet logistic regression analysis failed to identify significant dose association. To our knowledge we are the first to describe ALK rearrangements in post‐Chernobyl PTC samples using routine methods such as FISH and IHC. Our findings further support the hypothesis that gene rearrangements, but not oncogenic driver mutations, are associated with ionising radiation‐related tumour risk. IHC may represent an effective method for ALK‐screening in PTCs with known radiation aetiology, which is of clinical value since oncogenic ALK activation might represent a valuable target for small molecule inhibitors.
Lysobacter PilR, the Regulator of Type IV Pilus Synthesis, Controls Antifungal Antibiotic Production via a Cyclic di-GMP Pathway

ABSTRACT Lysobacter enzymogenes is a ubiquitous soil gammaproteobacterium that produces a broad-spectrum antifungal antibiotic, known as heat-stable antifungal factor (HSAF). To increase HSAF production for use against fungal crop diseases, it is important to understand how HSAF synthesis is regulated. To gain insights into transcriptional regulation of the HSAF synthesis gene cluster, we generated a library with deletion mutations in the genes predicted to encode response regulators of the two-component signaling systems in L. enzymogenes strain OH11. By quantifying HSAF production levels in the 45 constructed mutants, we identified two strains that produced significantly smaller amounts of HSAF. One of the mutations affected a gene encoding a conserved bacterial response regulator, PilR, which is commonly associated with type IV pilus synthesis. We determined that L. enzymogenes PilR regulates pilus synthesis and twitching motility via a traditional pathway, by binding to the pilA promoter and upregulating pilA expression. Regulation of HSAF production by PilR was found to be independent of pilus formation. We discovered that the pilR mutant contained significantly higher intracellular levels of the second messenger cyclic di-GMP (c-di-GMP) and that this was the inhibitory signal for HSAF production. Therefore, the type IV pilus regulator PilR in L. enzymogenes activates twitching motility while downregulating antibiotic HSAF production by increasing intracellular c-di-GMP levels. This study identifies a new role of a common pilus regulator in proteobacteria and provides guidance for increasing antifungal antibiotic production in L. enzymogenes. IMPORTANCE PilR is a widespread response regulator of the two-component system known for regulating type IV pilus synthesis in proteobacteria. Here we report that, in the soil bacterium Lysobacter enzymogenes, PilR regulates pilus synthesis and twitching motility, as expected. Unexpectedly, PilR was also found to control intracellular levels of the second messenger c-di-GMP, which in turn inhibits production of the antifungal antibiotic HSAF. The coordinated production of type IV pili and antifungal antibiotics has not been observed previously.
Physical and Magnetic Properties of Manganese Ferrite Nanoparticles

In this paper, manganese ferrite (MnFe2O4) nanoparticles were synthesized using chemical bath deposition (CBD) method and characterized by XRD and TEM to determine different properties of nanoparticles. The results obtained showed the formation of manganese ferrite nanoparticles with an average particle size are in good agreement with previous reported experimental results and displayed good magnetic properties. Magnetic properties was determined using Vibrating Sample Magnetometer(VSM). Due to simplicity and low cost of this process, it could be used for synthesis of ferrites nanoparticles. These materials may be used in drug delivery systems, electronic devices and water remediation.
Notice of RetractionEarnings Quality and the Agency Costs of Controlling Shareholder

High quality accounting information can help mitigate the information asymmetry of the Principal-Agent problem. So, it has a great effect on the corporate governance. Using instrumental variables and panel data model, this paper analyses how the earnings quality, measured by accrual quality, affects the agency costs of controlling shareholder empirically. It reveals that earnings quality affects the agency costs of controlling shareholder significantly; companies with higher earnings quality have lower agency costs of controlling shareholder, and the agency costs decreases with the improvement of earnings quality. The results provide new evidence on the corporate governance effect of accounting information.
Arctigenin Inhibits Liver Cancer Tumorigenesis by Inhibiting Gankyrin Expression via C/EBPα and PPARα

Burdock (Arctium lappa) is a popular vegetable in China and Japan that is consumed for its general health benefits. The principal active component of burdock is arctigenin, which shows a range of bioactivities in vivo and in vitro. Here, we investigated the potential anti-tumor effects of arctigenin using two human hepatocellular carcinoma (HCC) cell lines, HepG2 and Hep3B, and sought to elucidate its potential mechanisms of action. Our results showed that arctigenin treatment inhibited cell growth in both HepG2 and Hep3B cell lines (IC50 of 4.74 nM for HepG2 cells, and of 59.27 nM for Hep3B cells). In addition, migration, invasion, and colony formation by HepG2 cells were significantly inhibited by arctigenin. By contrast, treatment of Hep3B cells with arctigenin did not alter these parameters. Arctigenin also significantly reduced the levels of gankyrin mRNA and protein in HepG2 cells, but not in Hep3B cells. A luciferase assay indicated that arctigenin targeted the -450 to -400 region of the gankyrin promoter. This region is also the potential binding site for both C/EBPα and PPARα, as predicted and confirmed by an online software analysis and ChIP assay. Additionally, a co-immunoprecipitation (Co-IP) assay showed that binding between C/EBPα and PPARα was increased in the presence of arctigenin. However, arctigenin did not increase the expression of C/EBPα or PPARα protein. A binding screening assay and liquid chromatography–mass spectrometry (LC–MS) were performed to identify the mechanisms by which arctigenin regulates gankyrin expression. The results suggested that arctigenin could directly increase C/EBPα binding to the gankyrin promoter (-432 to -422 region), but did not affect PPARα binding. Expression of gankyrin, C/EBPα, and PPARα were analyzed in tumor tissues of patients using real-time PCR. Both C/EBPα and PPARα showed negative correlations with gankyrin. In tumor-bearing mice, arctigenin had a significant inhibitory effect on HCC growth. In conclusion, our results suggested that arctigenin could inhibit liver cancer growth by directly recruiting C/EBPα to the gankyrin promoter. PPARα subsequently bound to C/EBPα, and both had a negative regulatory effect on gankyrin expression. This study has identified a new mechanism of action of arctigenin against liver cancer growth.
A Handbook of Anatomy for Art Students

which we cannot agree, as, even assuming such a view to be correct, the extreme beauty of the illustrations, and the clearness and lucidity of the diction, entirely dissipate any erroneous notions of undue length. But such a view is not correct; the book is not one whit too long; it clearly demonstrates the outlines and curves of the body and their causes, thus enabling a student of art to take an intelligent interest in the why and the wherefore of his lights and shades, of his outlines and curves. But over and beyond this a second reading of this work in its latest edition has still further strengthened our opinion that it is essentially a work to be studied by anatomists themselves. A knowledge of its contents will enable the teacher to clothe his dry-as-dust and eternal " origins "
A review of graphene oxide, graphene buckypaper, and polymer/graphene composites: Properties and fabrication techniques

In this critical review, the preparation methods, unique properties, and graphene oxide structure as well as graphene have been interpreted. Various aspects for the graphene oxide reactivity were also discussed. Graphene oxide, usually obtained by exfoliating graphite, provides a way towards flexible, low-cost, bulk production paper-like materials known as buckypapers. Recently, graphene oxide and graphene buckypaper have been used as fillers for preparing composites. The buckypapers are freestanding thin porous network of carbon fillers bound by van der Waals interactions. Several techniques have been used to fabricate graphene oxide and graphene-based paper-like materials such as vacuum filtration method; spin coating technique, resin infiltration technique, evaporation-induced, and self-assembly technique. Among these, the most suitable technique is Langmuir-Blodgett assembly, where the graphene oxide paper formed is usually highly uniform. Free standing polymer/graphene oxide and polymer/graphene paper materials show remarkable mechanical stiffness and excellent flexibility combined with outstanding electrical conductivity. Incorporating polymer into graphene oxide and graphene buckypaper may modify the polymer and filler properties producing high performance materials. The buckypaper made from functionalized filler may form networks that facilitate penetrating matrix chains and result in composites with improved mechanical performance. Due to their outstanding physical, thermal, and electrical properties, these papers have promising applications such as; body armour, aerospace structure improvement, armoured vehicles, flexible energy storage devices, sensors, memory devices, transparent conductors, and photovoltaics.
Optimization of a Power Splitting Protocol for Two-Way Multiple Energy Harvesting Relay System

Energy harvesting (EH) combined with cooperative communications constitutes a promising solution for future wireless technologies. It enables additional efficiency and increased lifetime to wireless networks. This paper investigates a multiple-relay selection scheme for an EH-based two-way relaying (TWR) system. All relays are considered EH nodes that harvest energy from renewable energy and radio frequency (RF) sources. Some of them are selected to forward data to the destinations. The power splitting (PS) protocol, by which the EH node splits the input RF signal into two components for EH and information transmission, is adopted at the relay nodes. The objective is to jointly optimize: 1) the set of selected relays; 2) their PS ratios; and 3) their transmit power levels in order to maximize data rate-based utilities over multiple coherent time slots. A joint-optimization solution based on geometric programming (GP) and binary particle swarm optimization is proposed to solve non-convex problems for two utility functions reflecting the level of fairness in the TWR transmission. Numerical results illustrate the system’s behavior versus various parameters and show that the performance of the proposed scheme is very close to that of the optimal branch-and-bound method and that GP outperforms the dual problem-based method.
3D Jet Writing: Functional Microtissues Based on Tessellated Scaffold Architectures

The advent of adaptive manufacturing techniques supports the vision of cell‐instructive materials that mimic biological tissues. 3D jet writing, a modified electrospinning process reported herein, yields 3D structures with unprecedented precision and resolution offering customizable pore geometries and scalability to over tens of centimeters. These scaffolds support the 3D expansion and differentiation of human mesenchymal stem cells in vitro. Implantation of these constructs leads to the healing of critical bone defects in vivo without exogenous growth factors. When applied as a metastatic target site in mice, circulating cancer cells home in to the osteogenic environment simulated on 3D jet writing scaffolds, despite implantation in an anatomically abnormal site. Through 3D jet writing, the formation of tessellated microtissues is demonstrated, which serve as a versatile 3D cell culture platform in a range of biomedical applications including regenerative medicine, cancer biology, and stem cell biotechnology.