Miracle-dz/UniFAR-FTUs huggingface dataset:
CKI isoforms α and ε regulate Star–PAP target messages by controlling Star–PAP poly(A) polymerase activity and phosphoinositide stimulation

Star–PAP is a non-canonical, nuclear poly(A) polymerase (PAP) that is regulated by the lipid signaling molecule phosphatidylinositol 4,5 bisphosphate (PI4,5P2), and is required for the expression of a select set of mRNAs. It was previously reported that a PI4,5P2 sensitive CKI isoform, CKIα associates with and phosphorylates Star–PAP in its catalytic domain. Here, we show that the oxidative stress-induced by tBHQ treatment stimulates the CKI mediated phosphorylation of Star–PAP, which is critical for both its polyadenylation activity and stimulation by PI4,5P2. CKI activity was required for the expression and efficient 3′-end processing of its target mRNAs in vivo as well as the polyadenylation activity of Star–PAP in vitro. Specific CKI activity inhibitors (IC261 and CKI7) block in vivo Star–PAP activity, but the knockdown of CKIα did not equivalently inhibit the expression of Star–PAP targets. We show that in addition to CKIα, Star–PAP associates with another CKI isoform, CKIε in the Star–PAP complex that phosphorylates Star–PAP and complements the loss of CKIα. Knockdown of both CKI isoforms (α and ε) resulted in the loss of expression and the 3′-end processing of Star–PAP targets similar to the CKI activity inhibitors. Our results demonstrate that CKI isoforms α and ε modulate Star–PAP activity and regulates Star–PAP target messages.
Anger on the Internet: The Perceived Value of Rant-Sites

Despite evidence that anger is routinely expressed over the Internet via weblogs, social networking Web sites, and other venues, no published research has explored the way in which anger is experienced and expressed online. Consequently, we know very little about how anger is experienced in such settings. Two studies were conducted to explore how people experience and express their anger on a particular type of Web site, known as a rant-site. Study 1 surveyed rant-site visitors to better understand the perceived value of the Web sites and found that while they become relaxed immediately after posting, they also experience more anger than most and express their anger in maladaptive ways. Study 2 explored the emotional impact of reading and writing rants and found that for most participants, reading and writing rants were associated with negative shifts in mood.
Fast Switching in Magnetic Tunnel Junctions With Two Pinned Layers: Micromagnetic Modeling

Spin transfer torque random access memory is one of the promising candidates for future universal memory. The reduction of the current density required for switching and the increase of the switching speed are the most important challenges in this area. In this paper, a penta-layer structure with two pinned magnetic layers is studied by means of extensive micromagnetic calculations. By numerically investigating the dynamics of the switching process, a methodology of how to achieve fast and symmetric switching without a compensating magnetic field is presented. Our simulations also highlight the importance of the field acting perpendicular to the plane, which facilitates switching.
Differences in Access to Services in Rural Emergency Departments of Quebec and Ontario

Introduction Rural emergency departments (EDs) are important safety nets for the 20% of Canadians who live there. A serious problem in access to health care services in these regions has emerged. However, there are considerable geographic disparities in access to trauma center in Canada. The main objective of this project was to compare access to local 24/7 support services in rural EDs in Quebec and Ontario as well as distances to Levels 1 and 2 trauma centers. Materials and Methods Rural EDs were identified through the Canadian Healthcare Association's Guide to Canadian Healthcare Facilities. We selected hospitals with 24/7 ED physician coverage and hospitalization beds that were located in rural communities. There were 26 rural EDs in Quebec and 62 in Ontario meeting these criteria. Data were collected from ministries of health, local health authorities, and ED statistics. Fisher’s exact test, the t-test or Wilcoxon-Mann-Whitney test, were performed to compare rural EDs of Quebec and Ontario. Results All selected EDs of Quebec and Ontario agreed to participate in the study. The number of EDs visits was higher in Quebec than in Ontario (19 322 ± 6 275 vs 13 446 ± 8 056, p = 0.0013). There were no significant differences between Quebec and Ontario’s local population and small town population density. Quebec’s EDs have better access to advance imaging services such as CT scanner (77% vs 15%, p < .0001) and most the consultant support and ICU (92% vs 31%, p < .0001). Finally, more than 40% of rural EDs in Quebec and Ontario are more than 300 km away from Levels 1 and 2 trauma centers. Conclusions Considering that Canada has a Universal health care system, the discrepancies between Quebec and Ontario in access to support services are intriguing. A nationwide study is justified to address this issue.
Performance evaluation of reliable and unreliable opportunistic flooding in wireless sensor network

Opportunistic flooding [4] is an algorithm designed for wireless sensor network (WSN) intended to acquire significantly good performance on both flooding delay and energy consumption, considering both unreliable links and low-duty-cycle. On the other hand, end-to-end transfer schemes in WSNs can be categorized into acknowledgement based and non-acknowledgement based. Considering the reliable and unreliable implementations of opportunistic flooding algorithm, this paper investigates on the problems in the different end-to-end transmission schemes under low-duty-cycle networks to further optimize the performance of flooding in WSNs. Along with the simulation results and analysis, the characteristics and advisable environments of the reliable and unreliable opportunistic flooding have been provided.
The Fatty Acid Composition of Human Milk in Northern Nigeria

The authors previously reported that the milk of Yoruba women in southwestern Nigeria was deficient in •-linolenic acid and contained a high percentage (42%) of medium chain–length fatty acids (MCFA, C10-C14). In the present study, the authors used capillary gas-liquid chromatography to analyze the milk of Hausawomen in the northern region of Nigeria. The milk of the Hausa women contained 27% MCFA, 10.6% linoleic acid, 0.41% •-linolenic acid, 0.52% arachidonic acid, and 0.32% docosahexaenoic acid. The proportion of •-linolenic acid in the serum phospholipids of a subset of exclusively breastfed infants (n=15; mean age, 6.2 ± 0.3 months) was below the limit of detection (<0.03%). While the milk of women in northern Nigeria is adequate with regard to n-3 and n-6 fatty acids, to satisfy the requirements for •-linolenic acid, it may be necessary to supplement the infants of these women after the first 6 months of life.
Komárno: A Flagship of Symbolic Politics at the Slovak-Hungarian Border

Depuis le traite de Trianon, Komarno est, dans la politique symbolique slovaque et hongroise, beaucoup plus qu’une simple ville a la frontiere de l’ancien Etat dirigeant. Elle represente l’ensemble de la communaute hongroise en Slovaquie. Les auteures se fondent sur l’histoire orale tiree d’interviews qu’elles analysent en lien avec les politiques locales ou centrales des gouvernements slovaques et hongrois. Elles etudient plus precisement la perception qu’ont les Hongrois et les Slovaques de Komarno d’eux-memes et de leur environnement bi-ethnique. La cohabitation quotidienne au niveau local et l’instrumentalisation de la question nationale par Bratislava et Budapest sont mises en regard. Il apparait alors que l’evolution de la communaute bi-ethnique est entravee par la politisation de la question nationale, entendue comme une part de l’heritage post-communiste, comme si la frontiere physique, dont les contours ont disparu apres la chute du rideau de fer, devait etre remplacee par une frontiere symbolique linguistique et nationale.
Indoor localization based on bi-phase measurements for wireless sensor networks

Indoor localization is important for medical and industrial application as well as for wireless emergency and security systems. For such applications an accuracy within a few meters is desired. Available radio based systems within that accuracy are neither cost effective nor easy to deploy. In this work, we suggest an approach called bi-phase measurement based on phase measurements with two frequencies to determine the location of a tag. We design and build a complete indoor positioning system based on bi-phase measurements with easy to deploy and mobile wireless sensor nodes. The wireless sensor nodes form anchors and tags and communicate results to a location engine of the indoor positioning system. Our implementation comprises low-cost IEEE802.15. 4 radio chips with built-in support for phase measurements unit for both, anchor and tags. We compute the position of the tag based on distance estimation retrieved with biphase measurements. We evaluate our indoor positioning system providing first measurement results for accuracy and precision and discuss trade-off between scalability and accuracy.
Rapid Communication Identification and Characterization of Batk, a Predominantly Brain-Specific Non-Receptor Protein Tyrosine Kinase Related to Csk

A novel cDNA, brain-associated tyrosine kinase (Batk), was isolated from a rat hippocampal library and appears to encode a new member of the Csk subfamily of non-receptor protein tyrosine kinases, with 52% overall amino acid identity to rat Csk. Batk resembles kinases of the Src family in that it contains a Src homology 2 (SH2) domain and an SH3 domain, followed by a tyrosine kinase domain. Analysis of incompletely spliced Batk cDNAs suggests that the genomic structure of Batk is similar to that of Csk with identical exonhntron boundaries. Batk also shows significant homology (86% overall amino acid identity) to the recently described human megakaryocyte-spe-cific Matk. Although Batk is 41 amino acids shorter than Matk, Southern blot analysis suggests that Batk might be a rat homolog of Matk. Batk is predominantly expressed in the brain, with lower expression in the spleen and undetectable expression in other tissues. In situ hybridization and Northern blot analysis show that Batk is widely distributed throughout the adult brain, being primarily expressed in neurons, including those of the hippocampus and cortex. In contrast, embryos appear to have markedly decreased expression levels. Analysis of postnatal day 1 brain suggests that Batk may be upregulated at birth throughout the brain except in the cerebellum. In view of its homology to Csk, a negative regulator of Src family tyrosine kinases, and its generalized expression in the adult brain, we suggest that Batk may function as a brain-specific regulator of kinases involved in the development and maintenance of the nervous System.
Personality style and immediate help-seeking responses following the onset of myocardial infarction

Abstract The present set of studies investigated the relationship between personality style and behavioral and cognitive responses immediately following the onset of coronary symptoms among first-time myocardial infarction (MI) patients. A systematic replication of key findings across this study series suggested that individual differences in help-seeking behavior among acute MI patients may, in part, he attributable to personality style differences. The findings appear to have special relevance for patients at high risk for infarction in view of recent studies of delay time and mortality rates in thrombolytic intervention trials.
Molecular cloning and sequencing of salivary gland‐specific cDNAs of the blood‐sucking bug Triatoma brasiliensis (Hemiptera: Reduviidae)

Haematophagous insects produce pharmacological substances in their saliva to counteract vertebrate host haemostasis events such as coagulation, vasoconstriction and platelet aggregation. To investigate the bioactive salivary molecules of the triatomine bug Triatoma brasiliensis, we produced subtraction‐enriched cDNAs of salivary‐gland specific genes using suppression subtractive hybridization. Six full‐length differentially expressed cDNAs (Tb113, Tb125, Tb152, Tb169, Tb180 and Tb198) were selected, cloned and sequenced. Sequence similarity searches of the databases using the putative amino acid sequence of our clones gave the following results: Tb152 – Triabin, an antithrombin induced platelet aggregation factor found in salivary gland extracts of T. pallidipennis. Tb169 – Pallidipin, an anticollagen induced platelet aggregation factor also found in T. pallidipennis salivary homogenates. Tb180 – Procalin, the major allergen of T. protracta saliva. The other three salivary‐gland specific cDNAs produced no obvious homologies. Comparison of these salivary gland‐specific cDNAs of with those of other triatomines combined with functional studies using recombinant proteins will allow a better understanding of the co‐evolutionary process occurring between these insects and their vertebrate hosts, and may also lead to the discovery of novel antihaemostatic agents.
Image classification with saliency region and multi-task learning

In this paper, we propose a novel image classification method based on saliency region and multi-task learning (MTL). The proposed method has the advantages of both region-based and MTL-based methods. Specifically, we first detect the object region of an image with saliency detection technique, then extract its visual features, and then cluster them into groups, and finally employ MTL and SVM to build the classifier. Experimental results on two popular data sets (Corel and Caltech 101) have shown that our method has satisfactory classification performances and outperforms the traditional methods employing only region information or MTL.
Dairy Product Consumption and the Risk of Breast Cancer

It has been suggested in some reports that dairy product consumption may increase the risk of breast cancer. This review gives a brief overview of the etiology of breast cancer and in particular the roles of fat, bovine growth hormone, insulin-like growth factor-1 and estrogens. Evidence from animal studies and epidemiology does not support a role for fat in the etiology of breast cancer. The daily intake of insulin-like growth factor-1 and biologically active estrogens from dairy products is minute in comparison to the daily endogenous secretion of these factors in women, whereas bovine growth hormone is biologically inactive in humans. On the other hand, milk contains rumenic acid, vaccenic acid, branched chain fatty acids, butyric acid, cysteine-rich whey proteins, calcium and vitamin D; components, which have the potential to help prevent breast cancer. Evidence from more than 40 case-control studies and 12 cohort studies does not support an association between dairy product consumption and the risk of breast cancer.
QoS-aware bit scheduling in multi-user OFDM systems

Radio resource management (RRM) is widely regarded as a critical component in improving spectral efficiency of beyond third generation (3G) cellular air interfaces. Much of the published work in Orthogonal Frequency Division Multiplexing (OFDM) RRM focuses on exploiting multi-user, multi-channel and multi-application diversity. In this paper, we propose a Quality of Service (QoS)-aware bit scheduling framework to increase the flexibility and granularity of the resource allocation algorithms by adaptively matching the QoS requirements of the application bits of users to the characteristics of the OFDM subcarriers in a mixed-traffic environment. We show that with the finesse control of QoS-aware bit scheduling, it is possible to simultaneously achieve both an increase in user throughput and a reduction in user packet drop probability by accepting a within packet drop threshold increase in user latency. The performance gains obtainable are quantified in terms of user throughput, user latency, user jitter and user packet drop probability.
LARGE POST-BUCKLING DEFORMATIONS OF CYLINDRICAL SHELLS CONVEYING VISCOUS

This paper examines the post-buckling deformations of cylindrical shells conveying viscous fluid . The wall deformation is modelled using geometrically nonlinear shell theory , and lubrication theory is used to model the fluid flow . The coupled fluid – solid problem is solved using a parallelized FEM technique . It is found that the fluid – solid interaction leads to a violent collapse of the tube such that immediate opposite-wall contact occurs after the buckling if the volume flux is kept constant during buckling . If the pressure drop through the tube is kept constant during the buckling , the fluid – solid coupling slows down the collapse (compared to buckling under a dead load) . The ef fects of various parameters (upstream pressure , axial pre-stretch and the geometry of the tube) on the post-buckling behaviour are examined and the exact geometrically nonlinear shell theory is compared to Sanders’ (1963) moderate rotation theory . Finally , the implications of the results for previous models which described the wall deformation using so called ‘‘tube laws’’ are discussed .
Comparing Disproportionate Exposure to Acute and Chronic Pollution Risks: A Case Study in Houston, Texas

While environmental justice (EJ) research in the United States has focused primarily on the social distribution of chronic pollution risks, previous empirical studies have not analyzed disparities in exposure to both chronic (long‐term) and acute (short‐term) pollution in the same study area. Our article addresses this limitation though a case study that compares social inequities in exposure to chronic and acute pollution risks in the Greater Houston Metropolitan Statistical Area, Texas. The study integrates estimates of chronic cancer risk associated with ambient exposure to hazardous air pollutants from the Environmental Protection Agency's National‐Scale Air Toxics Assessment (2005), hazardous chemical accidents from the National Response Center's Emergency Response Notification System (2007–2011), and sociodemographic characteristics from the American Community Survey (2007–2011). Statistical analyses are based on descriptive comparisons, bivariate correlations, and locally derived spatial regression models that account for spatial dependence in the data. Results indicate that neighborhoods with a higher percentage of Hispanic residents, lower percentage of homeowners, and higher income inequality are facing significantly greater exposure to both chronic and acute pollution risks. The non‐Hispanic black percentage is significantly higher in neighborhoods with greater chronic cancer risk, but lower in areas exposed to acute pollution events. Households isolated by language—those highly likely to face evacuation problems during an actual chemical disaster—tend to reside in areas facing significantly greater exposure to high‐impact acute events. Our findings emphasize the growing need to examine social inequities in exposure to both chronic and acute pollution risks in future EJ research and policy.
Web 2.0 in education: a study of the explorative use of blogs with a postgraduate class

This paper outlines a study that explored educational applications of blogs with a class of postgraduate students in a Hong Kong university over a period of one semester in 2006, and considers its outcomes. Rather than using the usual learning management system to support learning in the class, the facilitator‐researcher organised a blog‐based environment where students accessed course material, posted reflections featuring artifacts created in the learning tasks, commented on each other's contributions, and in other ways participated on a regular basis throughout the semester. The study results suggest that when appropriately managed by a facilitator, blogs have the potential to effectively support teaching and learning activities. The experience from this study also suggests that in order to maximise teaching and learning opportunities, a blog system can be effectively expanded by the use of a variety of other Web 2.0 applications.
The Prevalence and Politics of HIV/AIDS in Zimbabwe: Examining the Ideological, Political and Historical Factors Behind the ‘Decline’

The reported reduction in the prevalence level of the Human Immunodeficiency Virus (HIV) in Zimbabwe has been represented as one of the most significant and rapid declines within any population since the epidemic emerged as a public health issue. This paper explains how this development has been reported, challenged and eventually owned by many of the diverse stakeholders who constitute Zimbabwe's overall AIDS response. The Zimbabwean government has claimed that the decline was brought about due to its own efforts and resources. However, while the country has received considerably less AIDS funding than its neighbours, external donations still account for the vast bulk of AIDS spending in Zimbabwe. In addition, instead of collapsing, as some predicted, the Zimbabwean state can also claim that it has presided over increased availability of antiretroviral therapy. In this paper we examine how various internal and external stakeholders deployed strategic explanations in an attempt to take credit for ‘the decline’; this is an important case study of how epidemiological data can be used to construct and contest political legitimacy.
PCR‐synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae

A PCR‐method for fast production of disruption cassettes is introduced, that allows the addition of long flanking homology regions of several hundred base pairs (LFH‐PCR) to a marker module. Such a disruption cassette was made by linking two PCR fragments produced from genomic DNA to kanMX6, a modification of dominant resistance marker making S. cerevisiae resistant to geneticin (G418). In a first step, two several hundred base pairs long DNA fragments from the 5′‐ and 3′‐region of a S. cerevisiae gene were amplified in such a way that 26 base pairs extensions homologous to the kanMX6 marker were added to one of their end. In a second step, one strand of each of these molecules then served as a long primer in a PCR using kanMX6 as template. When such a LFH‐PCR‐generated disruption cassette was used instead of a PCR‐made disruption cassette flanked by short homology regions, transformation efficiencies were increased by at least a factor of thirty. This modification will therefore also help to apply PCR‐mediated gene manipulations to strains with decreased transformability and/or unpredictable sequence deviations.