Miracle-dz/UniFAR-FTUs huggingface dataset:
Weight gain and metabolic risks associated with antipsychotic medications in children and adolescents.

BACKGROUND Antipsychotic-related weight gain and metabolic adverse effects have become a major focus, especially in youth. METHODS Review of randomized, cohort, and pharmacoepidemiologic studies of antipsychotic-related weight gain and metabolic adverse effects and of interventions for their reduction in youth. RESULTS Across 34 published head-to-head and placebo-controlled studies in youth with psychotic and bipolar disorders, weight gain ranged from 3.8 to 16.2 kg with olanzapine (n=353), 0.9-9.5 kg with clozapine (n=97), 1.9-7.2 kg with risperidone (n=571), 2.3-6.1 kg with quetiapine (n=133), and 0-4.4 kg with aripiprazole (n=451). In 24 placebo-controlled trials, the numbers-needed-to-harm for weight gain ≥7% in youth with bipolar disorder and schizophrenia were 39 (confidence interval [CI]: -1 to +6, not significant) for aripiprazole, 36 (CI: -1 to +7, not significant) for ziprasidone, 9 (CI: 7-14) for quetiapine, 6 (CI: 5-8) for risperidone, and 3 (CI: 3-4) for olanzapine. Data in youth with autism and disruptive behavior disorders, available only for some antipsychotics, suggest greater weight gain, possibly due to less prior antipsychotic exposure. Three-month results from a large cohort study in antipsychotic-naïve youth indicated that metabolic effects differ among second-generation antipsychotics, despite significant weight gain with all studied agents, suggesting additional, weight-independent effects. Further, pharmacoepidemiologic work indicates that antipsychotic polypharmacy increases the risk for obesity (odds ratio [OR]: 2.28 [CI: 1.49-3.65]) or any cardiovascular, cerebrovascular, or hypertensive adverse event (OR: 1.72 [CI: 1.10-2.69]). However, despite marked weight gain and its greater impact on youth, monitoring rates are low and studies of pharmacologic and behavioral interventions are extremely limited. CONCLUSIONS More research is needed to develop strategies to minimize antipsychotic-related weight gain and metabolic effects in youth and to discover treatments with lower risk potential.
Divorce in sub‐Saharan Africa: Are Unions Becoming Less Stable?

Divorce is one of the main drivers of family instability in sub-Saharan Africa. Using data from 101 Demographic and Health Surveys and novel estimation techniques, we 1) provide the first systematic estimates of divorce across 33 countries; 2) assess trends in divorce in 20 countries; and 3) investigate the key country-level correlates of divorce both across and within countries. Despite considerable geographic variation, our estimates show that divorce is common in most countries. Contrary to expectations, however, we find no evidence that divorce is increasing. Instead, divorce has been either stable or declining in recent decades. We show that socioeconomic factors associated with industrialization have countervailing effects on divorce. Urbanization and female employment are associated with higher levels of divorce, while age at first marriage and female education correspond to lower rates. These findings have implications for current and future family dynamics in sub-Saharan Africa.
Procaine Hydrochloride Fails to Relieve Pain in Patients with Acute Pancreatitis

Background: Several analgesics are in use for pain control in patients with acute pancreatitis. Procaine hydrochloride (procaine) has a long tradition and is recommended by the German Society of Gastroenterology and Metabolic Diseases for pain treatment in patients with acute pancreatitis. There is no controlled trial showing that procaine could be effective for pain treatment. Methods: In an open, randomized, controlled trial, 107 patients (76 male, 31 female; mean age 45 ± 12 years) were included and randomized either to receive procaine (n = 55) or pentazocine (n = 52) for pain relief. Procaine 2 g/ 24 h was administered by continuous intravenous infusion, pentazocine 30 mg was administered every 6 h as a bolus intravenous injection. Pentazocine was additionally administered on demand whenever required in patients of both treatment groups and its total consumption was recorded. Pain scores were assessed twice daily on a visual analogue scale. Results: Patients receiving procaine were significantly more likely to request additional analgesics compared to patients treated with pentazocine alone, 98 vs. 44%, respectively (p < 0.001). Procaine did not reduce the amount of pentazocine required for pain control. The amount of pentazocine given in both groups was not statistically significantly different. Recorded pain scores were significantly lower (p < 0.001) in patients in the pentazocine group during the first 3 days of analgesic treatment. From day 4 on there was no significant difference in pain scores among the two groups. Conclusion: Thus, intravenous procaine treatment is not effective for pain control in patients with acute pancreatitis.
Improved background subtraction techniques for security in video applications

This paper attempts to find moving objects by subtracting the background images from static single camera video sequences in security systems. It aims to improve the background subtraction techniques for indoor video surveillance applications. For dynamic video sequences, the object focus and object tracking are very difficult due to changing of various parameters such as camera noise, illumination changes, motion changes, and geometry of background changes. The background subtraction algorithm provides the solution to detect moving objects in the static scene. The recursive and non-recursive algorithms are discussed in an improved way. The novel automatic threshold updating (ATU) algorithm is also developed and tested for various indoor video sequences which gives better efficiency. The statistical and temporal differencing methods are also presented. Finally, our novel approach is compared with the existing methods.
Functional Roles of Loops 3 and 4 in the Cyclic Nucleotide Binding Domain of Cyclic AMP Receptor Protein from Escherichia coli *

Cyclic AMP is a ubiquitous secondary message that regulates a large variety of functions. The protein structural motif that binds cAMP is highly conserved with the exception of loops 3 and 4, whose structure and length are variable. The cAMP receptor protein of Escherichia coli, CRP, was employed as a model system to elucidate the functional roles of these loops. Based on the sequence differences between CRP and cyclic nucleotide gated channel, three mutants of CRP were constructed: deletion (residues 54–56 in loop 3 were deleted), insertion (loop 4 was lengthened by 5 residues between Glu-78 and Gly-79) and double mutants. The effects of these mutations on the structure and function of CRP were monitored. Results show that the deletion and insertion mutations do not significantly change the secondary structure of CRP, although the tertiary and quaternary structures are perturbed. The functional data indicate that loop 3 modulates the binding affinities of cAMP and DNA. Although the lengthened loop 4 may have some fine-tuning functions, the specific function of the original loop 4 of CRP remains uncertain. The function consequences of mutation in loop 3 of CRP are similar to that of site A and site B in the regulatory subunits of cyclic AMP-dependent protein kinases. Thus, the roles played by loop 3 in CRP may represent a more common mechanism employed by cyclic nucleotide binding domain in modulating ligand binding affinity and intramolecular communication.
Genetic risk score to predict biochemical recurrence after radical prostatectomy in prostate cancer: prospective cohort study

Purpose To investigate the genetic risk score (GRS) from a large-scale exome-wide association study as a tool of prediction for biochemical recurrence (BCR) after radical prostatectomy (RP) in prostate cancer (PCa). Results The 16 SNPs were selected as significant predictors of BCR. The GRS in men experiencing BCR was -1.21, significantly higher than in non-BCR patients (–2.43) (p < 0.001). The 10-year BCR-free survival rate was 46.3% vs. 81.8% in the high-versus low GRS group, respectively (p < 0.001). The GRS was a significant factor after adjusting for other variables in Cox proportional hazard models (HR:1.630, p < 0.001). The predictive ability of the multivariate model without GRS was 84.4%, increased significantly to 88.0% when GRS was included (p = 0.0026). Materials and Methods Total 912 PCa patients were enrolled who had received RP and genotype analysis using Exome chip (HumanExome BeadChip). Genetic results were obtained by the methods of logistic regression analysis which measured the odds ratio (OR) to BCR. The GRS was calculated by the sum of each weighted-risk allele count multiplied by the natural logarithm of the respective ORs. Survival analyses were performed using the GRS. We compared the accuracy of separate multivariate models incorporating clinicopathological factors that either included or excluded the GRS. Conclusions GRS had additional predictive gain of BCR after RP in PCa. The addition of personally calculated GRS significantly increased the BCR prediction rate. After validation of these results, GRS of BCR could be potential biomarker to predict clinical outcomes.
Preservation and evaluation of spiced chayote juice using hurdle technology

Abstract Chayote (Sechium edule) is a perennial climbing plant, belonging to the Cucurbitaceae family and is neglected or underutilized as a food or as a raw material in the food industry because of its bland taste. This bland taste can be overcome by blending with a suitable substrate such as sugarcane juice along with natural flavouring. However, this blending represents a preservation problem for the spiced juice, since both chayote juice and sugarcane juice are prone to microbial attack. Therefore, an attempt was made in the present study to preserve the spiced chayote juice by employing different hurdles i.e. pasteurization temperature (70 °C and 80 °C) and pasteurization time (10 and 20 min) followed by the addition of different concentrations of potassium metabisulphite (KMS) (200, 300 and 400 ppm). The samples so prepared were filled into glass bottles and stored under refrigeration for forty-five days. As the storage period proceeded there were significant decreases in pH, total protein content, total phenolic compounds, antioxidant activity, reducing power, metal chelation, tannin content, ascorbic acid content and overall acceptability, whereas there were significant increases in TSS (total soluble solids), titratable acidity, reducing sugars, total sugar, TPC (total plate count) and MIC (minimum inhibitory concentration). A cluster analysis of the data revealed that the pasteurization temperature exerted the most significant effect on the physicochemical and sensory characteristics of the spiced chayote juice as compared to the pasteurization time and KMS concentration, which failed to fall into the separate and respective clusters. In a nutshell, it was concluded that spiced chayote juice can be successfully preserved using hurdle technology (pasteurizing at 70 °C for 10 min followed by the addition of 400ppm KMS).
Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Invasion of surrounding normal tissues is generally considered to be a key hallmark of malignant (as opposed to benign) tumors. For some cancers in particular (e.g., brain tumors such as glioblastoma multiforme and squamous cell carcinoma of the head and neck – SCCHN) it is a cause of severe morbidity and can be life-threatening even in the absence of distant metastases. In addition, cancers which have relapsed following treatment unfortunately often present with a more aggressive phenotype. Therefore, there is an opportunity to target the process of invasion to provide novel therapies that could be complementary to standard anti-proliferative agents. Until now, this strategy has been hampered by the lack of robust, reproducible assays suitable for a detailed analysis of invasion and for drug screening. Here we provide a simple micro-plate method (based on uniform, self-assembling 3D tumor spheroids) which has great potential for such studies. We exemplify the assay platform using a human glioblastoma cell line and also an SCCHN model where the development of resistance against targeted epidermal growth factor receptor (EGFR) inhibitors is associated with enhanced matrix-invasive potential. We also provide two alternative methods of semi-automated quantification: one using an imaging cytometer and a second which simply requires standard microscopy and image capture with digital image analysis.
2G12-Expressing B Cell Lines May Aid in HIV Carbohydrate Vaccine Design Strategies

ABSTRACT The highly conserved cluster of high-mannose glycans on the HIV-1 envelope glycoprotein, gp120, has been highlighted as a target for neutralizing antibodies. 2G12, the first HIV-1 antiglycan neutralizing antibody described, binds with an unusual domain-exchanged structure that creates a high-affinity multivalent binding surface. It is an interesting challenge for rational vaccine design to generate immunogens capable of eliciting domain-exchanged 2G12-like responses. We recently showed that di-mannose recognition by the variable domains of 2G12 is independent of domain exchange but that exchange is critical for virus neutralization. Carbohydrate-based immunogens aimed at inducing 2G12-like antibodies may need to drive both di-mannose recognition and domain exchange through interactions with B cell receptors. Here we assessed the ability of such immunogens to activate mouse B cell lines displaying domain-exchanged wild-type 2G12 (2G12 WT), a non-domain-exchanged Y-shaped variant (2G12 I19R), and germ line 2G12 (2G12 gl). We show that several immunogens, including heat-killed yeast and bacteria, can activate both 2G12 WT and 2G12 I19R B cells. However, only discrete clusters of high-mannose glycans, as on recombinant forms of the HIV-1 envelope trimer and oligodendrons, activate 2G12 WT B cells. Furthermore, no immunogen tested activated 2G12 gl cells. Our results support the hypothesis that in order to drive domain exchange of an antimannose antibody response, a boost with an immunogen displaying discrete clusters of high-mannose glycans not recognized by conventional Y-shaped antibodies will be required. Additionally, a molecule capable of activating 2G12 gl cells might also be required. The results highlight broadly neutralizing antibody-expressing mouse B cells as potentially useful tools for carbohydrate immunogen screening.
FICUS: Fast Incremental Consistent Update in SDN based on relation graph

In Software Defined Networking (SDN), the configuration inconsistency during updates is one main source of network instability. An efficient updating scheme with configuration consistency is required. In this paper, we propose the scheme of Fast Incremental Consistent Update for SDN (FICUS) based on the relation graph (RG). In our scheme, we analyse the relation between update operations, construct the relation graph and find a proper order of these update operations to avoid inconsistency. To solve the problem, we define two types of relations: the path dependency relation and the path rejection relation. We evaluate our scheme and algorithms by comprehensive experiments. The results show that our scheme needs only 10%-40% of the rules compared with the two-phase update scheme and speeds up the update process by 40% in average.
Oil spill trajectory prediction with high-resolution ocean currents

ABSTRACT Simulated ocean currents from ocean circulation models along with the surface winds are generally used to force an oil spill trajectory model. Ocean circulation is a key factor in determining the drift of the spilled marine pollutants. The simulations of the drift pattern of spilled oil, when forced by the ocean currents from two ocean models, are presented here. Merchant Vessel (MV) Rak sunk at 72.4865°E, 18.7715°N, on 4 August 2011. As per the information from Indian Coast Guard, approximately 122.5 tons of fuel oil was spilled from the vessel from 5 August 2011 to 12 August 2011. An oil spill trajectory model, General NOAA Operational Modeling Environment (GNOME), was used to simulate the oil drift pattern from MV Rak from 1000 hours of 5 August 2011 to 1300 hours of 12 August 2011. GNOME was forced with winds from European Centre for Medium Range Weather Forecast (ECMWF) and ocean currents from Indian Ocean Forecasting System (INDOFOS) and High-resolution Operational Ocean Forecasting and reanalysis System (HOOFS). We found that the oil drift pattern obtained when forced with ocean currents from HOOFS was in better agreement with the actual track, compared to the one obtained while using INDOFOS currents.
Cerebral water and electrolytes. An experimental model of inappropriate secretion of antidiuretic hormone.

Administration of fluid and vasopressin injection to rats induced within 24 to 48 hours a condition which can be considered as an experimental model of the syndrome of inappropriate secretion of antidiuretic hormone (ADH). Concurrently with hyponatremia and hypoosmolarity in serum, the changes in muscle consisted of a fall in sodium content and a decrease in percentage dry weight equivalent to 12% swelling. There was no loss of potassium from muscle. In contrast, in brain the decrease in sodium content was smaller, the tissue percentage dry weight decreased only slightly, indicating minimal swelling, and there was a significant net loss of potassium. It is suggested that neurological dysfunction associated with the syndrome of inappropriate secretion of ADH is unlikely to be due to cerebral edema but may be related to the decreased potassium content of brain tissue.
Ki-67/MIB-1 as a Prognostic Marker in Cervical Cancer - a Systematic Review with Meta-Analysis.

BACKGROUND In cervical cancer patients it has been reported that there in a significant Ki-67/MIB-1 expression is correlated with survival in cervical cancer patients. However, the prognostic value is still not well understood. MATERIALS AND METHODS In the present meta-analysis the prognostic value of Ki-67/MIB-1 with regard to overall survival (OS) and disease-free survival (DFS) in cervical cancer was investigated. The databases of PubMed, ISI Web of Science, Cochrane Central Register of Controlled Trials, EMBASE, Science Direct and Wiley Online Library were used to identify appropriate literature. RESULTS In order to explore the relationship between Ki-67/ MIB-1 and cervical cancer, we have included 13 studies covering 894 patients in the current meta-analysis. The effect of Ki-67/MIB-1 on OS for pooled random effects HR estimate was 1.63 (95%confidence interval (CI) 1.09- 2.45; P<0.05). The pooled HR for DFS was 1.26 (95%CI 0.58-2.73; P>0.05) and the subgroup analysis indicated Ki-67/MIB1 was associated with DFS (HR=3.67, 95%CI 2.65-5.09) in Asians. CONCLUSIONS According to this meta-analysis, Ki-67/MIB-1 has prognostic value for OS in patients suffering from cervical cancer. For better evaluation of the prognostic role of Ki-67/MIB-1 on DFS, studies with larger numbers of patients are needed to validate present findings in the future.
Fall risk associated with inpatient medications.

PURPOSE The association between fall risk and inpatient medications was evaluated. METHODS A retrospective, case-control study was performed to compare the medication use of patients sustaining at least one fall during hospitalization (case group) with a control group of patients who did not fall. Data were collected from medical records and generated reports. A fall was defined by the hospital as an event in which the patient comes to rest on the floor from a lying, standing, or sitting position. Adult patients (≥18 years of age) admitted between January 1 and December 31, 2006, experiencing a fall at least 48 hours after hospital admission were included in the case group. Each case was matched with one control by age (within five years), sex, admission date (within 30 days), patient care unit, and length of stay. Medications administered within 48 hours before the fall for the case group or designated fall date and time for the control group were documented. RESULTS Of the 414 documented fall events, 209 patients met the inclusion criteria. Of those patients, 96 matched control patients on all criteria. Significantly more case patients received a greater number of central nervous system (CNS) agents compared with matched control patients (p = 0.017). There was no statistically significant difference in the number of medications from all other drug classes or the total number of medications received by the groups. CONCLUSION In a sample of hospitalized patients, CNS agents were significantly associated with falls.
Globalisation and the death of distance in social preferences ad inequity aversion: mpirical evidence from a pilot study on fair trade consumers

We analyze behaviour and motivations of a sample of about one thousand consumers purchasing “fair trade (FT) goods”, i. e. food and artisan goods which include socially responsible (SR) characteristics and (generally) a price premium for primary product producers with respect to equivalent non SR products. By estimating a simultaneous two-equation treatment regression model we find that FT products have less then unit income elasticity and their demand is negatively (positively) correlated with geographical distance from the nearest shop (age and awareness of SR criteria). Awareness of SR criteria depends in turn from a series of conditioning factors (consumption habits, membership of volunteer associations) which indirectly (via increased awareness) significantly affect consumption. We also measure consumers’ willingness to pay an extra amount just for the SR features of FT products (a direct measure of consumers social preferences when controlled for factors measuring specific pros and cons of FT products) and find that it is positively correlated with awareness of SR criteria.
Pyriform cell differentiation in Podarcis sicula is accompanied by the appearance of surface glycoproteins bearing α‐galNAc terminated chains

The present histochemical and cytochemical study using a lectin panel (WGA, GSI‐A4, GSI‐B4, PSA UEA‐I, PNA, LCA, Con‐A, DBA, MPA, BPA) has demonstrated that, in Podarcis sicula, the differentiation of small follicle cells into pyriform cells by means of intermediate cells is accompanied by the appearance of glycoproteins bearing α‐GalNAc terminated O‐linked side chains on the cell surface. The distribution of DBA‐ and MPA‐binding sites over the follicular epithelium changed during the different stages of oocyte growth. DBA‐ and MPA‐binding sites ffirst appeared at the beginning of folliculogenesis within the zona pellucida (ZP) and on the surface of small cells, i.e., the stem cells of pyriform cells. Afterward, labeling was evident on the cell surfaces of intermediate cells and, later on, also of pyriform cells. On the other hand, no labeling was detected on the small cells located under the basal lamina, which, reportedly, do not differentiate into pyriform cells (Filosa et al. J. Embryol. Exp. Morphol., 1979 ; 15:297‐316). Once pyriform cells were differentiated, the distribution of DBA‐ and MPA‐binding sites over the follicular epithelium remained unchanged until intermediate and pyriform cells underwent apoptosis (Motta et al. J. Exp. Zool., 1996 ; 276:233‐241) and the follicular epithelium transformed into a monolayer composed of small follicle cells only (Filosa Mon. Zool. Ital., 1973 ; 7:151–165). During this stage of oocyte growth, DBA and MPA labeling gradually decreased to completely disappear in the follicular epithelium of vitellogenic follicles. It is noteworthy that the observed changes in the distribution of DBA‐ and MPA‐binding sites represent the ffirst evidence recognized by lectins of a gradual modification of surface glycoprotein distribution over the follicular epithelium in the ovarian follicles of nonmammalian vertebrates so far studied. Finally, the zona pellucida (ZP), characterized by the presence of GalNAc, GluNAc, Man, and Gal, was demonstrated to be first synthetized by the oocyte and later on by the follicle cells. Anat Rec 263:1–9, 2001. © 2001 Wiley‐Liss, Inc.