Miracle-dz/UniFAR-FTUs huggingface dataset:
What promotes and inhibits cooperation in mental health care across disciplines, services and service sectors? A qualitative study

Aims. To explore the practice of cooperation in mental health care across services and identify conditions that promote and inhibit cooperation. Methods. Focus groups with relevant service providers were conducted in four exemplary regions in Germany (rural/urban and East/West). The discussions were content analysed. Results. Seven central categories were deduced: (1) involved service providers, (2) comprehension, (3) reasons, (4) instruments for cooperation, (5) promoting and inhibiting conditions, (6) interdisciplinarity, and (7) regional and personal networks. Cooperation is practiced in networks, rather than defined relations. Who is involved in cooperation depends on patients/clients needs and may vary from case to case. Service providers do not have theoretical concepts of cooperation. Cooperation relies not only on personal contacts and knowledge between services but also on time, financial reimbursement and the social capital of the environment. In particular, cooperation with physicians was considered to be difficult by non-medical professionals. Physician's role models do not seem to include cooperation with other disciplines as a core task. Conclusions. To improve cooperation, regional and sustainable mental health networks have to be systematically implemented by providing leadership, time and reimbursement for network meetings. Interdisciplinary cooperation practice should be part of the curricula of medical students and residents in psychiatry.
Defining a strategy to introduce a software product line using existing embedded systems

Engine Control Systems (ECS) for automobiles have numerous variants for many manufactures and different markets. To improve development efficiency, exploiting ECS commonalities and predicting their variability are mandatory. The concept of software product line engineering meets the business background of ECS. However, we should carefully investigate the expected technical, economical, and organizational effects of introducing this strategy into existing products.This paper explains an approach for assessing the potential of merging existing embedded software into a product line approach. The definition of an economically useful product line approach requires two things: analyzing return on investment (ROI) expectations of a product line and understanding the effort required for building reusable assets. We did a clone analysis to provide the basis for effort estimation for merge potential assessment of existing variants. We also report on a case study with ECS. We package the lessons learned and open issues that arose during the case study.
A simple and effective method for predicting travel times on freeways

We present a method to predict the time that will be needed to traverse a given section of a freeway when the departure is at a given time in the future. The prediction is done on the basis of the current traffic situation in combination with historical data. We argue that, for our purposes, the current traffic situation of a section of a freeway is well summarized by the current status travel time. This is the travel time that would result if one were to depart immediately and no significant changes in traffic would occur. This current status travel time can be estimated from single- or double-loop detectors, video data, probe vehicles, or any other means. Our prediction method arises from the empirical observation that there exists a linear relationship between any future travel time and the current status travel time. The slope and intercept of this relationship may change subject to the time of day and the time until departure, but linearity persists. This observation leads to a prediction scheme by means of linear regression with time-varying coefficients.
Sequential Complexity as a

We propose string compressibility as a descriptor of temporal structure in audio, for the purpose of determining musical similarity. Our descriptors are based on computing track-wise compression rates of quantised audio features, using multiple temporal resolutions and quantisation granularities. To verify that our descriptors capture musically relevant information, we incorporate our descriptors into similarity rating prediction and song year prediction tasks. We base our evaluation on a dataset of 15 500 track excerpts of Western popular music, for which we obtain 7 800 web-sourced pairwise similarity ratings. To assess the agreement among similarity ratings, we perform an evaluation under controlled conditions, obtaining a rank correlation of 0.33 between intersected sets of ratings. Combined with bag-offeatures descriptors, we obtain performance gains of 31.1% and 10.9% for similarity rating prediction and song year prediction. For both tasks, analysis of selected descriptors reveals that representing features at multiple time scales benefits prediction accuracy.
The folate pool in colorectal cancers is associated with DNA hypermethylation and with a polymorphism in methylenetetrahydrofolate reductase.

PURPOSE Aberrant DNA methylation occurs in a subset of colorectal cancers and is characterized by regional areas of hypermethylation at CpG islands. The aims of this study were firstly to evaluate the levels of folate intermediates (FIs) in tumors with aberrant DNA methylation and secondly to determine whether these levels are affected by polymorphisms in key genes involved in folate metabolism. EXPERIMENTAL DESIGN The concentrations of two major intracellular FIs, 5,10-methylenetetrahydrofolate and tetrahydrofolate (FH4), were measured in 103 surgically resected colorectal cancers. DNA hypermethylation at seven different CpG islands was measured using the MethylLight assay. Genotyping for polymorphisms in the thymidylate synthase, cystathionine beta-synthase, methionine synthase, and methylenetetrahydrofolate reductase (MTHFR) genes was carried out using PCR and PCR-RFLP. RESULTS Significantly higher levels of FH4 were found in tumors from the proximal colon compared with those originating in the distal colon and rectum. Tumors with aberrant DNA methylation of CpG islands within promoter regions of the hMLH1, TIMP3, and ARF genes also contained higher levels of both 5,10-methylenetetrahydrofolate and FH4. In contrast, patients who were homozygous for the C667T polymorphism of the MTHFR gene had significantly lower concentrations of both these FIs in their tumor tissue. CONCLUSIONS The concentrations of FIs in colorectal tumors are directly related to the presence of frequent DNA hypermethylation and inversely related to the presence of a common polymorphism in the MTHFR gene. FIs could serve as biochemical markers for the risk of developing this disease, as well as for the prediction of toxicity and efficacy of fluorouracil-based treatments.
Dickkopf-3 Upregulates VEGF in Cultured Human Endothelial Cells by Activating Activin Receptor-Like Kinase 1 (ALK1) Pathway

Dkk-3 is a member of the dickkopf protein family of secreted inhibitors of the Wnt pathway, which has been shown to enhance angiogenesis. The mechanism underlying this effect is currently unknown. Here, we used cultured HUVECs to study the involvement of the TGF-β and VEGF on the angiogenic effect of Dkk-3. Addition of hrDkk-3 peptide (1 or 10 ng/ml) to HUVECs for 6 or 12 h enhanced the intracellular and extracellular VEGF protein levels, as assessed by RTPCR, immunoblotting, immunocytochemistry and ELISA. The increase in the extracellular VEGF levels was associated to the VEGFR2 activation. Pharmacological blockade of VEGFR2 abrogated Dkk-3-induced endothelial cell tubes formation, indicating that VEGF is a molecular player of the angiogenic effects of Dkk-3. Moreover, Dkk-3 enhanced Smad1/5/8 phosphorylation and recruited Smad4 to the VEGF gene promoter, suggesting that Dkk-3 activated ALK1 receptor leading to a transcriptional activation of VEGF. This mechanism was instrumental to the increased VEGF expression and endothelial cell tubes formation mediated by Dkk-3, because both effects were abolished by siRNA-mediated ALK1 knockdown. In summary, we have found that Dkk-3 activates ALK1 to stimulate VEGF production and induce angiogenesis in HUVECs.
In vitro and in vivo antimicrobial activity of combined therapy of silver nanoparticles and visible blue light against Pseudomonas aeruginosa

Silver nanoparticles (AgNPs) have been used as potential antimicrobial agents against resistant pathogens. We investigated the possible therapeutic use of AgNPs in combination with visible blue light against a multidrug resistant clinical isolate of Pseudomonas aeruginosa in vitro and in vivo. The antibacterial activity of AgNPs against P. aeruginosa (1×105 colony forming unit/mL) was investigated at its minimal inhibitory concentration (MIC) and sub-MIC, alone and in combination with blue light at 460 nm and 250 mW for 2 hours. The effect of this combined therapy on the treated bacteria was then visualized using transmission electron microscope. The therapy was also assessed in the prevention of biofilm formation by P. aeruginosa on AgNP-impregnated gelatin biopolymer discs. Further, in vivo investigations were performed to evaluate the efficacy of the combined therapy to prevent burn-wound colonization and sepsis in mice and, finally, to treat a real infected horse with antibiotic-unresponsive chronic wound. The antimicrobial activity of AgNPs and visible blue light was significantly enhanced (P<0.001) when both agents were combined compared to each agent alone when AgNPs were tested at MIC, 1/2, or 1/4 MIC. Transmission electron microscope showed significant damage to the cells that were treated with the combined therapy compared to other cells that received either the AgNPs or blue light. In addition, the combined treatment significantly (P<0.001) inhibited biofilm formation by P. aeruginosa on gelatin discs compared to each agent individually. Finally, the combined therapy effectively treated a horse suffering from a chronic wound caused by mixed infection, where signs of improvement were observed after 1 week, and the wound completely healed after 4 weeks. To our knowledge, this combinatorial therapy has not been investigated before. It was proved efficient and promising in managing infections caused by multidrug resistant bacteria and could be used as an alternative to conventional antibiotic therapy.
Complex Distal Humerus Fractures—Comparison of Polyaxial Locking and Nonlocking Screw Configurations—A Preliminary Biomechanical Study

Objective: The investigation hypothesized that in current anatomical precontoured plates, angular stability plays only a minor role for the efficacy of the osteosynthesis at the distal humerus. Methods: An AO C2.3 fracture model was simulated and osteosynthesis performed with plates positioned in parallel. System rigidity and median fatigue limit were analyzed in artificial bones and the cycles to failure in cadaver specimens. Loads were applied in anterior–posterior direction (75° flexion) and axial direction (5° flexion). Four composite bone groups were investigated as follows: (1) 2.7 mm polyaxial locking screws, (2) 3.5 mm polyaxial locking screws, (3) 3.5 mm polyaxial locking screws and a gap bridging screw, and (4) 2.7 mm nonlocking screws. Two cadaver groups were investigated with 3.5 mm diameter polyaxial locking (5) versus nonlocking screws (6). Results: There were no differences in stiffness found between the locking versus nonlocking constructs in artificial (1) versus (4) and in cadaver bones (5) versus (6). The larger screw diameter of 3.5 mm in combination with a gap bridging screw significantly increased construct stiffness by 25% (3). The median fatigue limit was significantly increased using larger screw diameters (2) and a gap bridging screw (3). In cadaver bones, the polyaxial locking screws constructs (5) resisted higher peak loads and more cycles until failure compared with nonlocking constructs (6). Conclusions: System stiffness increases with larger screw diameters and becomes significant with additional gap bridging screws in artificial bones. The use of polyaxial locking screws in anatomical adapted plates becomes more important in poor bone quality.
Crystal Structure of the Catalytic Domain of the PknB Serine/Threonine Kinase from Mycobacterium tuberculosis *

With the advent of the sequencing programs of prokaryotic genomes, many examples of the presence of serine/threonine protein kinases in these organisms have been identified. Moreover, these kinases could be classified as homologues of those belonging to the well characterized superfamily of the eukaryotic serine/threonine and tyrosine kinases. Eleven such kinases were recognized in the genome of Mycobacterium tuberculosis. Here we report the crystal structure of an active form of PknB, one of the four M. tuberculosis kinases that are conserved in the downsized genome of Mycobacterium leprae and are therefore presumed to play an important role in the processes that regulate the complex life cycle of mycobacteria. Our structure confirms again the extraordinary conservation of the protein kinase fold and constitutes a landmark that extends this conservation across the evolutionary distance between high eukaryotes and eubacteria. The structure of PknB, in complex with a nucleotide triphosphate analog, reveals an enzyme in the active state with an unprecedented arrangement of the Gly-rich loop associated with a new conformation of the nucleotide γ-phosphoryl group. It presents as well a partially disordered activation loop, suggesting an induced fit mode of binding for the so far unknown substrates of this kinase or for some modulating factor(s).
A colour display technique for NMR imaging.

A method of producing a full colour representation of an NMR image from a monochrome storage oscilloscope is described. Variations in the proton density are displayed as changes in colour hue by decoding the image data into three sets of intensity information which correspond to the three primary colour separations of the image. These are displayed consecutively on the oscilloscope and recombined photographically on to colour film.
Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall

Insect galls may be study models to test the distribution of pectins and arabinogalactan-proteins (AGPs) and their related functions during plant cell cycles. These molecules are herein histochemically and immunocitochemically investigated in the kidney-shaped gall induced by Baccharopelma dracunculifoliae (Psyllidae) on leaves of Baccharis dracunculifolia DC. (Asteraceae) on developmental basis. The homogalacturonans (HGAs) (labeled by JIM5) and the arabinans (labeled by LM6) were detected either in non-galled leaves or in young galls, and indicated stiffening of epidermal cell walls, which is an important step for cell redifferentiation. The labeling of HGAs by JIM7 changed from young to senescent stage, with an increase in the rigidity of cell walls, which is important for the acquaintance of the final gall shape and for the mechanical opening of the gall. The variation on the degree of HGAs during gall development indicated differential PMEs activity during gall development. The epitopes recognized by LM2 (AGP glycan) and LM5 (1–4-β-D-galactans) had poor alterations from non-galled leaves towards gall maturation and senescence. Moreover, the dynamics of pectin and AGPs on two comparable mature kidney-shaped galls on B. dracunculifolia and on B. reticularia revealed specific peculiarities. Our results indicate that similar gall morphotypes in cogeneric host species may present distinct cell responses in the subcelular level, and also corroborate the functions proposed in literature for HGAs.
Multi-View Facial Expression Recognition Based on Group Sparse Reduced-Rank Regression

In this paper, a novel multi-view facial expression recognition method is presented. Different from most of the facial expression methods that use one view of facial feature vectors in the expression recognition, we synthesize multi-view facial feature vectors and combine them to this goal. In the facial feature extraction, we use the grids with multi-scale sizes to partition each facial image into a set of sub regions and carry out the feature extraction in each sub region. To deal with the prediction of expressions, we propose a novel group sparse reduced-rank regression (GSRRR) model to describe the relationship between the multi-view facial feature vectors and the corresponding expression class label vectors. The group sparsity of GSRRR enables us to automatically select the optimal sub regions of a face that contribute most to the expression recognition. To solve the optimization problem of GSRRR, we propose an efficient algorithm using inexact augmented Lagrangian multiplier (ALM) approach. Finally, we conduct extensive experiments on both BU-3DFE and Multi-PIE facial expression databases to evaluate the recognition performance of the proposed method. The experimental results confirm better recognition performance of the proposed method compared with the state of the art methods.
Standard and Higher Dose of Olanzapine in Patients With Schizophrenia or Schizoaffective Disorder: A Randomized, Double-Blind, Fixed-Dose Study

Abstract The objective of this study was to assess the dose-response relationship of standard and higher doses of olanzapine in a randomized, double-blind, 8-week, fixed-dose study comparing olanzapine 10 (n = 199), 20 (n = 200), and 40 mg/d (n = 200) for patients with schizophrenia or schizoaffective disorder and suboptimal response to current treatment. Patients meeting criteria for antipsychotic treatment resistance were excluded. Dose-response relationship was assessed by linear regression analysis with log-transformed dose (independent variable) and Positive and Negative Syndrome Scale (PANSS) total score (dependent variable). There were no significant dose group differences in patients completing the study (overall, 67.8%). All dose groups showed statistically significant improvement in PANSS total scores from baseline to end point without significant dose-response relationship (P = 0.295). Post hoc analysis of response showed significant interaction between baseline PANSS and dose (P = 0.023), indicating better response at higher doses for patients with higher baseline PANSS. There was a significant dose response for mean change in weight (P = 0.003) with significant difference between the 10- and 40-mg-dose groups (P = 0.002; 1.9 [10 mg/d], 2.3 [20 mg/d], and 3.0 kg [40 mg/d]). There was a significant dose response for change in prolactin (P < 0.001) with a significant difference between each group (−10.5 [10 mg/d], −1.7 [20 mg/d], and 4.9 ng/mL [40 mg/d]; P ≤ 0.018). Over 8 weeks, non-treatment-resistant patients with schizophrenia or schizoaffective disorder responded to all 3 doses of olanzapine, without a statistically significant dose-response relationship, suggesting that for many patients with schizophrenia or schizoaffective disorder, particularly those who are mildly or moderately ill, 10 mg/d should be the initial dose of choice.
A Channel Estimation Method Base on Selection of CTIR Length in MIMO-OFDM Systems

The multiple transmit and receive antennas can be used in OFDM systems to improve communication quality and capacity.In this paper,a channel estimation method base on selection of channel time impulse response (CTIR) length for MIMO-OFDM system is investigated. Simulation results both in terms of mean square error(MSE) and symbol error rate(SER) via Monte Carlo show that Comparing with Conventional LS and MMSE algorithm, the performance of proposed MMSE estimation algorithm outperforms Conventional LS , inferiors to Conventional MMSE,but its calculation complexity is lower than Conventional MMSE,thereby,the compromise is performed between performance and complexity by selection of CIR length.
Network topology identification from spectral templates

Network topology inference is a cornerstone problem in statistical analyses of complex systems. In this context, the fresh look advocated here permeates benefits from convex optimization and graph signal processing, to identify the so-termed graph shift operator (encoding the network topology) given only the eigenvectors of the shift. These spectral templates can be obtained, for example, from principal component analysis of a set of graph signals defined on the particular network. The novel idea is to find a graph shift that while being consistent with the provided spectral information, it endows the network structure with certain desired properties such as sparsity. The focus is on developing efficient recovery algorithms along with identifiability conditions for two particular shifts, the adjacency matrix and the normalized graph Laplacian. Application domains include network topology identification from steady-state signals generated by a diffusion process, and design of a graph filter that facilitates the distributed implementation of a prescribed linear network operator. Numerical tests showcase the effectiveness of the proposed algorithms in recovering synthetic and structural brain networks.
A NHERF binding site links the βPDGFR to the cytoskeleton and regulates cell spreading and migration

The Na+/H+ exchanger regulatory factor, NHERF, is a multifunctional adapter protein involved in a wide range of physiological activities. NHERF associates with merlin and the ezrin/radixin/moesin (MERM) family of membrane-actin cytoskeletal linker proteins through its C-terminus and is capable of interacting via its PDZ1 domain to the βPDGF receptor (βPDGFR). Thus, NHERF, potentially links the βPDGFR to the actin cytoskeleton through its interaction with MERM proteins. In the present study, we have examined whether abolishing the interaction of βPDGFR with NHERF results in actin cytoskeletal rearrangements. We have stably expressed a wild-type βPDGFR, a mutant βPDGFR (L1106A) that is incapable of interacting with NHERF, as well as a kinase defective mutant receptor (K634R), in PDGFR-deficient mouse embryonic fibroblasts. Our observations indicate that cells expressing βPDGFR (L1106A) were impaired in their ability to spread and migrate on fibronectin compared with wild-type and K634R cells. L1106A mutant cells also revealed an increased number of focal adhesions, a condensed F-actin ring at the cell periphery and a decrease in total focal adhesion kinase (FAK) tyrosine phosphorylation. Further, we show that NHERF and MERM proteins could act as intermediary bridging proteins between βPDGFR and FAK. Thus, the interaction of βPDGFR with NHERF may provide an essential link between the cell membrane and the cortical actin cytoskeleton independent of receptor activity.
Study on route optimization of logistics distribution based on ant colony and genetic algorithm

Ant Colony Optimization (ACO) algorithm and genetic algorithms (GA) are two commonly used methods dealing with vehicle route optimizing. According to the characteristics of the two methods, by combining the two algorithms, a hybrid algorithm is proposed to solve the vehicle routing problem, avoiding the disadvantages of long time searching, easily falling into local optimal solution in ACO and the shortcomings of iterative redundancy, inefficiency in GA. Some experimental results prove that the hybrid optimization algorithm (HOA) is feasible and efficient in solving the problem of vehicle route optimization in logistics distribution.