Miracle-dz/UniFAR-FTUs huggingface dataset:
Central melatonin binding sites in rainbow trout (Onchorhynchus mykiss).

A combination of in vitro autoradiography and membrane homogenate receptor assays has been used to localize and characterized 2-[125I]iodomelatonin binding sites in the brain of the rainbow trout (Onchorhynchus mykiss). Specific 2-[125I]iodomelatonin binding, defined as that displaced by 1 microM melatonin, increased linearly with increasing protein concentration in membrane homogenates of whole trout brain. Specific binding was both time and temperature dependent and reversible in the presence of 1 microM melatonin. Binding was saturable at between 100-150 pM 2-[125I]iodomelatonin and Scatchard analysis of saturation isotherms revealed a dissociation constant (Kd) of 15.00 +/- 0.95 pM and a maximum receptor number (Bmax) of 42.35 +/- 2.70 fm/mg protein (n = 16). Addition of 10(-4) M GTP gamma S (an analogue of guanosine triphosphate) to saturation isotherms apparently reduced the Bmax by 75% on average with no apparent change in the affinity of the binding. Scatchard analysis of saturation isotherms generated from whole brain membrane homogenates of trout kept on long days (15 hr light:9 hr dark) and killed either during the midlight or middark phase showed no significant differences in either the Kd or the Bmax of 2-[125I]iodomelatonin binding, although a robust rhythm in melatonin concentration was confirmed in these fish. Displacement of 2-[125I]iodomelatonin binding with increasing concentrations of competing ligands gave an order of potency of 2-iodomelatonin > melatonin >> 5-HT. Localization of specific central 2-[125I]iodomelatonin binding in the rainbow trout showed high levels of binding associated with neuronal areas involved in the processing of visual signals, particularly the optic tectum and nucleus rotundus. (ABSTRACT TRUNCATED AT 250 WORDS)
Behavioral Health Care for Adolescents with Poorly Controlled Diabetes via Skype: Does Working Alliance Remain Intact?

Background: Increasingly various technologies are being tested to deliver behavioral health care. Delivering services via videoconferencing shows promise. Given that the patient-provider relationship is a strong predictor of patient adherence to medical regimens, addressing relationship quality when services are not delivered face-to-face is critical. To that end, we compared the therapeutic alliance when behavioral health care was delivered to youth with poorly controlled type 1 diabetes mellitus (T1DM) and their caregivers in-clinic with the same services delivered via Internet-based videoconferencing (i.e., Skype™). Methods: Seventy-one adolescents with poorly controlled T1DM (hemoglobin A1c ≤9%) and one of their caregivers received up to 10 sessions of a family-based behavioral health intervention previously shown to improve adherence to diabetes regimens and family functioning; 32 were randomized to the Skype condition. Youth and caregivers completed the working alliance inventory (WAI), a 36-item measure of therapeutic alliance, at the end of treatment. Additionally, the number of behavioral health sessions completed was tracked. Results: No significant differences in WAI scores were found for those receiving behavioral health care via Skype versus in-clinic. Youth WAI goal and total scores were significantly associated with the number of sessions completed for those in the clinic group. Conclusion: Behavioral health can be delivered to youth with T1DM via Internet-based videoconferencing without significantly impacting the therapeutic relationship. Thus, for those adolescents with T1DM who require specialized behavioral health care that targets T1DM management, Internet-based teleconferencing represents a viable alternative to clinic-based care.
Course and predictors of pain and physical functioning in patients with hip osteoarthritis: Systematic review and meta-analysis.

OBJECTIVE To systematically summarize the literature on: (i) the course of pain and physical functioning; and (ii) predictors of deterioration of pain and physical functioning in patients with osteoarthritis of the hip. METHODS A literature search was conducted in PubMed, CINAHL, Embase, PsychINFO and SPORTDiscus up to July 2015. Meta-analyses and qualitative data syntheses were performed. RESULTS Eleven of the 15 included studies were of high quality. With regard to the course of pain and physical functioning, high heterogeneity was found across studies (I² > 71%) and within study populations (reflected by large standard deviations (SDs) of change scores). Therefore, the course of pain and physical functioning was interpreted to be indistinct. Clinical characteristics (higher comorbidity count and presence of knee osteoarthritis), health behaviour factors (no supervised exercise and physical inactivity) and socio-demographics (lower education) were found to predict deterioration of pain (weak evidence). Higher comorbidity count and lower vitality were found to predict deterioration of physical functioning (strong evidence). For several other predictive factors weak evidence was found (e.g. bilateral hip pain, increase in hip pain (change), bilateral knee pain, presence of knee osteoarthritis). CONCLUSION Because of high heterogeneity across studies and within study populations, no conclusions can be drawn with regard to the course of pain and physical functioning. Several clinical characteristics, health behaviours and psychosocial factors prognosticate deterioration of pain and physical functioning. These findings may guide future research aimed at the identification of subgroups of patients with hip osteoarthritis.
Efficacy of short-term dynamic psychotherapy : past, present, and future.

The author outlines the history of brief dynamic psychotherapy, describes some of its characteristics, and addresses methodological requirements for assessing the efficacy of psychotherapy. Review of two major meta-analyses suggests that manual-based brief dynamic psychotherapy by trained therapists is likely to be as effective as other forms of psychotherapy and more effective than no treatment. More studies are needed that 1) compare brief dynamic psychotherapy with other forms of treatment for specific psychiatric disorders; 2) use theory-specific measures of outcome in addition to measures of symptoms; and 3) compare brief dynamic psychotherapy with long-term psychotherapy.
Optimal scheduling of load tap changer and switched shunt capacitors in smart grid with electric vehicles and charging stations

Random charging of plug-in electric vehicles (PEVs) particularly during the peak load hours could impairment the performance of future smart grids. This paper presents genetic algorithms (GAs) for optimal scheduling of LTC and switched shunt capacitors (SSCs) to improve the performance of smart grid with PEV charging at consumer premises in residential feeders and PEV charging stations (PEV-CSs) in distribution networks. The forecasted daily load curves associated with PEV-CSs and residential feeders populated with PEVs are first generated and then incorporated in the GA-based optimal LTC and SSC scheduling solution. Simulation results without and with optimal scheduling are presented for a 449 node smart grid system with 5 PEV-CSs considering random and coordinated charging of 264 PEVs in 22 low voltage residential networks.
The effects of storage condition on starch metabolism and regeneration potentials of twin-scales and inflorescence stem explants of Narcissus tazetta

SummaryThe natural propagation rate of Narcissus is very slow. In vitro micropropagation of Narcissus is more efficient than conventional propagation for rapidly building up aseptic stocks of varieties, especially for the establishment of new cvs. and the production of pathogen-free stock material. In the present study, Narcissus tazetta cv. ‘Ziva’ bulbs were used as the source of mother plants. The bulbs were kept at 30°C in a dark chamber until the start of the experiments. Prior to explant preparation, the bulbs were subjected to a cold treatment at 15°C in the dark for 6 wk to break dormancy. Twin-scales and inflorescence stem discs were isolated from aseptic bulb parts and were used as the initial explants. The polar orientation affected the regeneration of the inflorescence stem. Storage duration at 30°C followed by cold treatment were found to affect starch levels, adenosine diphosphate glucose pyrophosphorylase (AGPase) activities, and regeneration potentials. Starch levels were reduced significantly during a 10 mo. storage period at 30°C in both twin-scales and inflorescence stem disc explants. Regeneration was followed by an efficient acclimatization system with 98–99% survival. More than 500 highly uniform young bulbs were propagated from one mother bulb in a 12 mo. period.
Modular modeling using Petri nets

In this paper, we introduce a class of Petri nets with input and output transitions called extended controllable-output (ECO) nets. These nets are live and reversible. By appropriate control, it is possible to realize the output transitions in any given ratio while keeping the marking bounded. These properties are highly needed from a manufacturing point of view. Furthermore, we show that the integration of such Petri nets preserves these properties under some fairly weak conditions as the integrated model is also an ECO net.
Evidence for Long Range Allosteric Interactions between the Extracellular and Cytoplasmic Parts of Bacteriorhodopsin from the Mutant R82A and Its Second Site Revertant R82A/G231C*

Evidence is presented for long range interactions between the extracellular and cytoplasmic parts of the heptahelical membrane protein bacteriorhodopsin in the mutant R82A and its second site revertant R82A/G231C. (i) In the double mutants R82A/G72C and R82A/A160C, with the cysteine mutation on the extracellular or cytoplasmic surface, respectively, the photocycle is the same as in the single mutant R82A with an accelerated deprotonation of the Schiff base and a reversed order of proton release and uptake. Proton release and uptake kinetics were measured directly at either surface by using the unique cysteine residue as attachment site for the pH indicator fluorescein. Whereas in wild type proton uptake on the cytoplasmic surface occurs during the M-decay (τ ∼ 8 ms), in R82A it occurs already during the first phase of the M-rise (τ < 1 μs). (ii) The introduction of a second mutation at the cytoplasmic surface in position 231 (helix G) restores wild type ground state absorption properties, kinetics of photocycle and of proton release, and uptake in the mutant R82A/G231C. In addition, kinetic H/D isotope effects provide evidence that the proton release mechanism in R82A/G231C and in wild type is similar. These results suggest the existence of long range interactions between the cytoplasmic and extracellular surface domains of bacteriorhodopsin mediated by salt bridges and hydrogen-bonded networks between helices C (Arg-82) and G (Asp-212 and Gly-231). Such long range interactions are expected to be of functional significance for activation and signal transduction in heptahelical G-protein-coupled receptors.
On the status of Buyang presyllables: A Response to Professor Ho Dah-An

INTRODUCTION In his review of Baxter and Sagart (2014), Ho (2016:210-7) objects to the use of the Buyang word of Chinese origin ma l 0 ɔk11 'deer' as evidence for reconstructing a presyllable in the word 鹿 luwk 'deer' in Chinese. He argues instead that this word, while of Chinese origin, was borrowed through the mediation of Zhuang, and offers two competing hypotheses to explain the first syllable ma0-. First, he proposes that this ma0could be a Buyang prefix added after borrowing took place. Second, he suggests that Buyang ma l 0 ɔk11 'deer' was borrowed as a whole from Zhuang maxloeg 'deer', itself borrowed from Chinese 马鹿 mǎlù 'red deer'. In this paper, I evaluate Prof. Ho's claims from the point of view of Kra-Dai historical phonology. First, I show that his assertion that Buyang ma l 0 ɔk11 'deer' is a Zhuang loanword is due to a misunderstanding of the cited source. Second, I present evidence that at least some Buyang presyllables are archaic features that should be reconstructed back to proto-Kra-Dai. Third, I discuss the reconstruction of Chinese 鹿 luwk 'deer' in the light of the first two sections.
A 21-dBm $I/Q$ Digital Transmitter Using Stacked Output Stage in 28-nm Bulk CMOS Technology

This paper proposes the use of a high-power stacked output stage for a current-based in-phase/quadrature (<inline-formula> <tex-math notation="LaTeX">$I/Q$ </tex-math></inline-formula>) direct digital to RF modulator (DDRM) in bulk CMOS. The main nonlinearities associated with implementing the stacked transistor on top of the <inline-formula> <tex-math notation="LaTeX">$I/Q$ </tex-math></inline-formula> DDRM are easily compensated by a simple 2-D digital predistortion. A prototype implemented in 28-nm bulk CMOS achieves a saturated output power (<inline-formula> <tex-math notation="LaTeX">$P_{\textrm {SAT}}$ </tex-math></inline-formula>) of 25 dBm and a peak output power (<inline-formula> <tex-math notation="LaTeX">$P_{\textrm {out}}$ </tex-math></inline-formula>) of 21 dBm at 1-GHz carrier frequency (<inline-formula> <tex-math notation="LaTeX">$f_{c}$ </tex-math></inline-formula>). Their corresponding efficiencies are 45% power added efficiency and 33% system efficiency (<inline-formula> <tex-math notation="LaTeX">$\eta _{\mathrm{ sys}}$ </tex-math></inline-formula>), respectively. In addition, it achieves 11.5% <inline-formula> <tex-math notation="LaTeX">$\eta _{\mathrm{ sys}}$ </tex-math></inline-formula> with a −30.5-dB error vector magnitude when transmitting a 40-MHz 64 quadrature amplitude modulation wireless local area network (WLAN) signal. The WLAN signal is transmitted at 12-dBm average <inline-formula> <tex-math notation="LaTeX">$P_{\textrm {out}}$ </tex-math></inline-formula>, and at 1-GHz <inline-formula> <tex-math notation="LaTeX">$f_{c}$ </tex-math></inline-formula> with 8.73-dB peak to average power ratio (peak <inline-formula> <tex-math notation="LaTeX">$P_{\textrm {out}}$ </tex-math></inline-formula> of 20.73 dBm).
Adaptive and maladaptive functioning in Kleefstra syndrome compared to other rare genetic disorders with intellectual disabilities

Detailed neurobehavioural profiles are of major value for specific clinical management, but have remained underexposed in the population with intellectual disabilities (ID). This was traditionally classified based on IQ level only. Rapid advances in genetics enable etiology based stratification in the majority of patients, which reduces clinical heterogeneity. This paper illustrates that specific profiles can be obtained for rare syndromes with ID. Our main aim was to study (mal)adaptive functioning in Kleefstra Syndrome (KS) by comparing and contrasting our findings to three other subgroups: Koolen‐de Vries Syndrome, GATAD2B‐related syndrome, and a mixed control group of individuals with ID. In total, we studied 58 individuals (28 males, 30 females) with ID; 24 were diagnosed with KS, 13 with Koolen‐de Vries Syndrome, 6 with the GATAD2B‐related syndrome, and 15 individuals with undefined neurodevelopmental disorders. All individuals were examined with a Vineland Adaptive Behavior Scale, mini PAS‐ADD interview, and an Autism Diagnostic Observation Schedule to obtain measures of adaptive and maladaptive functioning. Each of the three distinctive genetic disorders showed its own specific profile of adaptive and maladaptive functioning, while being contrasted mutually. However, when data of the subgroups altogether are contrasted to the data of KS, such differences could not be demonstrated. Based on our findings, specific management recommendations were discussed for each of the three syndromes. It is strongly suggested to consider the genetic origin in individuals with congenital neurodevelopmental disorders for individual based psychiatric and behavioral management.
Aslib Journal of Information Management An exploratory study of health scientists ’ data reuse behaviors : examining attitudinal , social , and resource factors

Purpose: The main purpose of this exploratory study is to examine how health scientists’ attitudinal, social, and resource factors affect their data reuse behaviors. Design/methodology/approach: A survey method was utilized to investigate to what extent attitudinal, social, and resource factors influence health scientists’ data reuse behaviors. The health scientists’ data reuse research model was validated by using Partial Least Squares (PLS) based Structural Equation Modeling (SEM) technique with a total of 161 health scientists in U.S. Findings: Analysis of the results showed that health scientists’ data reuse intentions are driven by attitude toward data reuse, community norms of data reuse, disciplinary research climate, and organizational support factors. This research also found that both the perceived usefulness of data reuse and the perceived concern involved in data reuse have a significant influence on health scientists’ attitude toward data reuse. Research limitations/implications: This research evaluated its newly proposed research model, based on the theory of planned behavior, using a sample from the Community of Science Scholars database. This research showed an overall picture of how attitudinal, social, and resource factors influence health scientists’ data reuse behaviors. This research is limited by the study’s sample size and low response rate, and is therefore considered as an exploratory study rather than a confirmatory study. Practical implications: This research suggested that diverse strategies need to be utilized to promote health scientists’ data reuse behaviors in the contexts of health science research communities, academic institutions, and libraries. Originality/value: This is one of the initial studies in scientific data reuse which provides a holistic view of health scientists’ data sharing behaviors. The findings of this study provide the groundwork for strategies to facilitate data reuse practice in the health sciences.
Patterns of cytokine expression in AIDS‐related non‐Hodgkin's lymphoma

The pathogenesis of AIDS‐related non‐Hodgkin's lymphomas (AIDS‐NHL) involves accumulation of genetic lesions, stimulation and selection by antigen, as well as infection by viruses. Deregulation of cytokine loops has also been proposed to contribute to AIDS‐NHL development, although data are available only for a limited number of cytokines. In this study we have utilized a panel of AIDS‐NHL cell lines to investigate in detail the pattern of tumour expression and production of a wide spectrum of cytokines. The cytokines investigated included interleukin (IL)‐1α, IL‐1β, IL‐2, IL‐3, IL‐4, IL‐5, IL‐6, IL‐7, IL‐8, IL‐10, IL‐13, TNFα, TNFβ, IFNγ, TGFβ2, G‐CSF, GM‐CSF and SCF. The AIDS‐NHL cell lines utilized were representative of both AIDS‐related Burkitt lymphoma (AIDS‐BL) and AIDS‐related body cavity‐based lymphoma (AIDS‐BCBL). Overall, AIDS‐NHL were found to produce IL‐6, IL‐10 and TNFβ, although with different patterns depending upon the biological features of the tumour. Production of high levels of IL10 preferentially associated with Epstein‐Barr virus (EBV) positive AIDS‐BL and AIDS‐BCBL, although lower levels of the cytokine were also detectable among EBV‐negative AIDS‐BL. Production of IL‐6 was restricted to EBV‐positive AIDS‐BL and AIDS‐BCBL, whereas it was absent among EBV‐negative AIDS‐BL. Production of TNFβ clustered with AIDS‐BL, whereas this was absent among AIDS‐BCBL. These results define that the pattern of cytokine expression of AIDS‐NHL depends upon the biological features of the tumour and may have implications for the pathogenesis of these disorders.
Allergens from mites: implications of cross‐reactivity between invertebrate antigens

Group 1 and 2 allergens are probably the most important mite allergens. Some mite allergens, such as tropomyosin, are widely cross‐reactive. The prevalence of sensitization to invertebrates cannot be based solely on skin test or serologic data with the allergen extracts currently available. Purified (recombinant) allergens, selected for low cross‐reactivity, are urgently needed.
Abnormal synaptic Ca2+ homeostasis and morphology in cortical neurons of familial hemiplegic migraine type 1 mutant mice

Migraine is among the most common and debilitating neurological conditions. Familial hemiplegic migraine type 1 (FHM1), a monogenic migraine subtype, is caused by gain‐of‐function of voltage‐gated CaV2.1 calcium channels. FHM1 mice carry human pathogenic mutations in the α1A subunit of CaV2.1 channels and are highly susceptible to cortical spreading depression (CSD), the electrophysiologic event underlying migraine aura. To date, however, the mechanism underlying increased CSD/migraine susceptibility remains unclear.
Multiple Face Recognition Using Local Features and Swarm Intelligence

Face recognition plays an important role in security applications, but in real-world conditions face images are typically subject to issues that compromise recognition performance, such as geometric transformations, occlusions and changes in illumination. Most face detection and recognition works to date deal with single face images using global features and supervised learning. Differently from that context, here we propose a multiple face recognition approach based on local features which does not rely on supervised learning. In order to deal with multiple face images under varying conditions, the extraction of invariant and discriminative local features is achieved by using the SURF (Speeded-Up Robust Features) approach, and the search for regions from which optimal features can be extracted is done by an improved ABC (Artificial Bee Colony) algorithm. Thresholds and parameters for SURF and improved ABC algorithms are determined experimentally. The approach was extensively assessed on 99 different still images – more than 400 trials were conducted using 20 target face images and still images under different acquisition conditions. Results show that our approach is promising for real-world face recognition applications concerning different acquisition conditions and transformations. key words: local features, iterative search, face recognition
Toward Industrially Usable Agent Technology for Smart Grid Automation

This work is aimed at facilitating the industrial adoption of agent technology. This paper proposes the hybrid agent architecture specific to the power system automation domain. The architecture builds on the logical-node concept of IEC 61850 and comprises a deliberative and a reactive layer, combining the advantages of both. By relying on the underlined industrial standards IEC 61850 and IEC 61499, the architecture ensures practical applicability and captures domain-specific concepts in the agent-based system design. The developed agent-based system was validated in the cosimulation framework. The architecture provides for rapid system development, reducing the software development life cycle. The benefits are in the traceability of the software requirements, reuse of software components, ease of redesign, and direct deployment of the system model.