Miracle-dz/UniFAR-FTUs huggingface dataset:
Properties of R1162, a broad-host-range, high-copy-number plasmid

Regions of plasmid DNA encoding characteristic properties of the IncQ (P-4) group plasmid R1162 were identified by mutagenesis and in vitro cloning. Coding sequences sufficient for expression of incompatibility and efficient conjugal mobilization by plasmid R751 were found to be linked to the origin of DNA replication. In contrast, there was a region remote from the origin, and active in trans, that was required for plasmid maintenance. A derivative that was temperature sensitive for stability was isolated. The defect mapped at or near the region required for plasmid maintenance and resulted in far fewer copies of supercoiled plasmid DNA per cell under permissive conditions. A second region required for stability was also identified from the behavior of a deletion derivative of R1162, which did not, however, show an altered number of supercoiled plasmid DNA copies. Finally, a plasmid DNA mutation resulting in a substantially higher copy number was isolated. Plasmid reconstruction experiments suggested that the mutation was linked to the replicative origin.
An Empirical Investigation into Knowledge Management in Pakistani Think Tanks

Knowledge management can play a central strategic role in organisational life, potentially initiating from strategy planning to performance achievement. The continuous adjustment of organisational strategy and performance can be powerful in knowledge intensive organisations such as think tanks. Think tanks are policy research organisations, usually of small to medium size, and non-profit in nature. The Pakistani think tank ‘industry’ is at an important tipping point in terms of establishing knowledge ‘impact’ for the state and the wider society. Think tank organisations are gaining dual attention for creating awareness in society and suggesting policies to the state. The central purpose of this research is to provide an explanation of why and how knowledge management plays a role within think tanks, especially with regards to organisations linking strategy and performance. It also seeks to provide evidence from Pakistani think tanks providing suggestions for organisational improvement that may be applicable to think tanks in other developing countries. A thorough literature review from the field of knowledge management includes knowledge, knowledge management, and the strategic relationship of knowledge management, organisational strategy and organisational performance. Specifically, the research seeks to analyse knowledge management in social sciences policy research think tanks based in Islamabad, the capital of Pakistan. The participation of eight think tanks provided access for field visits to conduct interviews, observations and document collection. From the findings, it is recommended that consciousness towards knowledge management can highlight the natural and independent process to keep organisational strategy and performance improving continuously in a cyclic manner. Knowledge resources with Pakistani think tanks demand proper recognition and valuation, as they are potentially being worth more compared to material resources. Amongst the knowledge resources, cultural values and norms, multilingual skills, ‘friendly’ internal environments and leadership are found to be important. The strength of Pakistani think tanks is in their Relational Capital, which they appear to be utilising. Informal-Tacit knowledge management practices are highlighted more in the context, though gaps do appear to exist for formal practices. It is suggested that think tanks could further enhance real visibility through their Relational Capital and making use of tacit informal practices leading to friendly, multilingual and effectively leadership cultures with positive values. Think tanks have the potential to help provide solutions during turbulent times, both leading and managing knowledge – effecting a positive impact upon both state and society.
Sistem Informasi Pendaftaran Pernikahan Berbasis Web Pada Kantor Urusan Agama Kecamatan Banyumas

Abstract - The use of internet for the needs of online registration has begun to develop in Indonesia Archipelago. People can access information and register marriage more easily and quickly through the internet without having to visit KUA first. Through the website KUA officers can easily view the marriage registration data. Registration of marriage to KUA Banyumas Subdistrict still using manual registration method by way of bride to visit KUA Banyumas Sub-district then do face-to-face registration with KUA officer so that less effective and efficient time. The registration information system through the internet or online is a good approach to improving accessibility to the community. The research method used is quantitative and qualitative. The Office of Religious Affairs of Banyumas Sub-district is an Office under the Ministry of Religious Affairs that conducts marriage services for the community around Banyumas Sub-district and provides information for the ease of the community in order to access more effectively and efficiently for prospective marriage applicants. Keywords: Information System, Online Registration System, KUA District Banyumas Abstrak - Penggunaan internet untuk kebutuhan pendaftaran online sudah mulai berkembang di Nusantara Indonesia. Masyarakat bisa mengakses informasi dan melakukan pendaftaran pernikahan dengan lebih mudah dan cepat melalui internet tanpa harus berkunjung ke KUA terlebih dahulu. Melalui website petugas KUA bisa dengan mudah melihat data pendaftar pernikahan. Pendaftaran pernikahan pada KUA Kecamatan Banyumas masih menggunakan metode pendaftaran manual yaitu dengan cara calon pengantin mendatangi KUA Kecamatan Banyumas lalu melakukan pendaftaran secara tatap muka dengan petugas KUA sehingga kurang efektif dan efisien dalam manajemen waktu. Sistem informasi pendaftaran melalui internet atau online merupakan suatu pendekatan yang baik terhadap peningkatan kemudahan akses untuk masyarakat. Metode penelitian yang digunakan adalah kuantitatif dan kualitatif. Kantor Urusan Agama Kecamatan Banyumas merupakan sebuah Kantor dibawah Kementerian Agama Republik Indonesia yang secara langsung bertugas melakukan pelayanan pernikahan untuk masyarakat sekitar Kecamatan Banyumas dan memberikan informasi kepada masyarakat agar dapat mengakses dengan lebih efektif dan efisien bagi calon pendaftar pernikahan. Kata Kunci : Sistem Informasi, Sistem Pendaftaran online, KUA Kecamatan Banyumas
B S : a spatial shape-based scale-independent simulation environment for biological systems

The simulation and visualization of biological system models is becoming more and more important both in clinical use and in basic research. Since many systems are characterized by interactions involving different scales at the same time, several approaches have been defined to handle such complex systems at different spatial and temporal scale. In this context, we propose BS, a 3D particle-based spatial simulator whose novelty consists of providing a uniform and geometry-oriented multiscale modeling environment. These features make BS “scaleindependent”, able to express geometric and positional information, and able to support transformations between scales simply defined as mappings between different granularity model instances. To highlight BS peculiarities, we sketch a multiscale model of human aortic valve where shapes are used at the cell scale for describing the interaction between a single valvular interstitial cell and its surrounding matrix, at the tissue scale for modeling the valve leaflet tissue mechanical behaviour, and at the organ scale for reproducing, as a 3D structure with fluid-structure interaction, the motion of the valve, blood, and surrounding tissue.
Conditional Deletion of Focal Adhesion Kinase Leads to Defects in Ventricular Septation and Outflow Tract Alignment

ABSTRACT To examine a role for focal adhesion kinase (FAK) in cardiac morphogenesis, we generated a line of mice with a conditional deletion of FAK in nkx2-5-expressing cells (herein termed FAKnk mice). FAKnk mice died shortly after birth, likely resulting from a profound subaortic ventricular septal defect and associated malalignment of the outflow tract. Additional less penetrant phenotypes included persistent truncus arteriosus and thickened valve leaflets. Thus, conditional inactivation of FAK in nkx2-5-expressing cells leads to the most common congenital heart defect that is also a subset of abnormalities associated with tetralogy of Fallot and the DiGeorge syndrome. No significant differences in proliferation or apoptosis between control and FAKnk hearts were observed. However, decreased myocardialization was observed for the conal ridges of the proximal outflow tract in FAKnk hearts. Interestingly, chemotaxis was significantly attenuated in isolated FAK-null cardiomyocytes in comparison to genetic controls, and these effects were concomitant with reduced tyrosine phosphorylation of Crk-associated substrate (CAS). Thus, it is possible that ventricular septation and appropriate outflow tract alignment is dependent, at least in part, upon FAK-dependent CAS activation and subsequent induction of polarized myocyte movement into the conal ridges. Future studies will be necessary to determine the precise contributions of the additional nkx2-5-derived lineages to the phenotypes observed.
Path Analysis of the Related Factors to Subjective Well-Being of Primary and Middle School Principals—Based on Job Demands-Resources Model

Objective: The objective was to investigate path analysis of work-family conflict, job salary and promotion satisfaction, job burnout to subjective well-being of primary and middle school principals, and provide advices for enhancing their subjective well-being. Methods: Totally 300 primary and middle school principals in Chongqing completed the WFC, JSPSS, MBI-GS and GWB. Results: There were four significant paths: 1) work-family conflict could influence subjective well- being directly (β = -0.125, P -0.197, total effect was -0.322), job burnout partially mediated the relation between work-family conflict and subjective well-being; 3) job burnout could influence subjective well-being directly (β = -0.529, P
Hormone predictors of bone mineral density changes during the menopausal transition.

UNLABELLED OBJECTIVE AND CONTEXT: Our objective was to examine predictability of reproductive hormone concentrations for bone mineral density (BMD) loss during the menopausal transition. DESIGN We conducted a longitudinal (five annual examinations), multiple-site (n = 5) cohort study, the Study of Women's Health Across the Nation (SWAN). PARTICIPANTS Participants included, at baseline, 2311 premenopausal or early perimenopausal African-American, Caucasian, Chinese, and Japanese women. MAIN OUTCOME MEASURES We assessed annual dual-energy x-ray absorptiometry lumbar spine and total hip BMD measures, with endogenous estradiol (E2), FSH, androgens, and self-reported menstrual bleeding patterns. RESULTS Over the 4-yr period, lumbar spine BMD loss was 5.6% in natural postmenopause, 3.9% in surgical postmenopause, or 3.2% in late perimenopause. Baseline FSH concentrations, subsequent FSH levels, and their interaction predicted 4-yr BMD loss. If baseline FSH was less than 25 mIU/ml, higher follow-up FSH (>70 mIU/ml) predicted a 4-yr spine BMD loss of -0.05 g/cm(2). If baseline FSH values were more than 35-45 mIU/ml, lower follow-up FSH (i.e. 40-50 mIU/ml) predicted a -0.05 g/cm(2) 4-yr spine BMD loss. Charts show amounts of predicted BMD losses with combinations of baseline FSH values and FSH levels over time. E2 concentrations less than 35 pg/ml were associated with lower BMD, but annual E2 measures and changes did not predict BMD loss. Testosterone, free androgen index, and dehydroepiandrosterone sulfate concentrations were not significantly associated with BMD loss. CONCLUSIONS Spine and hip BMD losses during the menopause transition were most strongly related to the interaction between initial FSH levels and longitudinal FSH changes and not to E2 or androgen levels or changes.
Pengaruh Manajemen Pengetahuan Terhadap Inovasi: Kasus Industri IT di Indonesia

The purpose of this journal is to examine the effect of the relationship between knowledge management and innovation in companies based on IT in Indonesia. This study uses the SEM (Structured Equation Modeling) method using PLS software based on primary data from the questionnaire. Based on the results of the study, it was found that the influence of knowledge management on innovation was significantly positive. This can be seen from the relationship between dimensions that is quite strong, where knowledge management has a very positive impact on the dimensions of process innovation and dimensions of product innovation. The proposed framework can be used to guide the development of strategies for implementing knowledge management to enhance the competitiveness of companies through innovation.
Étude comparative de méthodes ultrasonores non linéaires appliquées à des modèles expérimentaux de scellement et/ou d'ostéointégration de prothèses

Ce travail de these s'inscrit dans la perspective a long terme d'implementer in vivo de nouvelles methodes non invasives de controle du scellement ou de l'osteointegration de protheses osseuses (implants dentaires, protheses de hanche). Bien qu'etant la plus utilisee cliniquement, la radiographie a rayons X souffre d'une faible sensibilite. Le potentiel de methodes developpees ces vingt dernieres annees et fondees sur des mesures d'elasticite, a ete demontre in vitro, mais leur efficacite in vivo est encore sujette a caution. Notre objectif a ete d'evaluer le potentiel de nouvelles methodes basees sur la mesure de la reponse elastique non lineaire, methodes qui sont apparues dans les domaines de la geophysique et du controle non destructif, et qui ont montre une plus grande sensibilite que la reponse lineaire elastique a la presence de contacts faibles au sein d'une structure rigide, tels que les fissures. Pour cela, plusieurs modeles d'osteointegration et de scellement (avec conditions aux limites ou conditions d'amortissement variees) ont donc ete etudies experimentalement a l'aide de differentes techniques d'elasticite non lineaire. Au final, cette etude experimentale a permis l'extraction des parametres non lineaires les plus prometteurs et d'evaluer leurs avantages et limites respectifs en vue de leur application in vivo.
Tissue Microarray Sampling Strategy for Prostate Cancer Biomarker Analysis

High-density tissue microarrays (TMA) are useful for profiling protein expression in a large number of samples but their use for clinical biomarker studies may be limited in heterogeneous tumors like prostate cancer. In this study, the optimization and validation of a tumor sampling strategy for a prostate cancer outcomes TMA is performed. Prostate cancer proliferation determined by Ki-67 immunohistochemistry was tested. Ten replicate measurements of proliferation using digital image analysis (CAS200, Bacus Labs, Lombard, IL, USA) were made on 10 regions of prostate cancer from a standard glass slide. Five matching tissue microarray sample cores (0.6 mm diameter) were sampled from each of the 10 regions in the parallel study. A bootstrap resampling analysis was used to statistically simulate all possible permutations of TMA sample number per region or sample. Statistical analysis compared TMA samples with Ki-67 expression in standard pathology immunohistochemistry slides. The optimal sampling for TMA cores was reached at 3 as fewer TMA samples significantly increased Ki-67 variability and a larger number did not significantly improve accuracy. To validate these results, a prostate cancer outcomes tissue microarray containing 10 replicate tumor samples from 88 cases was constructed. Similar to the initial study, 1 to 10 randomly selected cores were used to evaluate the Ki-67 expression for each case, computing the 90th percentile of the expression from all samples used in each model. Using this value, a Cox proportional hazards analysis was performed to determine predictors of time until prostate-specific antigen (PSA) recurrence after radical prostatectomy for clinically localized prostate cancer. Examination of multiple models demonstrated that 4 cores was optimal. Using a model with 4 cores, a Cox regression model demonstrated that Ki-67 expression, preoperative PSA, and surgical margin status predicted time to PSA recurrence with hazard ratios of 1.49 (95% confidence interval [CI] 1.01–2.20, p = 0.047), 2.36 (95% CI 1.15–4.85, p = 0.020), and 9.04 (95% CI 2.42–33.81, p = 0.001), respectively. Models with 3 cores to determine Ki-67 expression were also found to predict outcome. In summary, 3 cores were required to optimally represent Ki-67 expression with respect to the standard tumor slide. Three to 4 cores gave the optimal predictive value in a prostate cancer outcomes array. Sampling strategies with fewer than 3 cores may not accurately represent tumor protein expression. Conversely, more than 4 cores will not add significant information. This prostate cancer outcomes array should be useful in evaluating other putative prostate cancer biomarkers.
Murine and Chicken Chondrocytes Regulate Osteoclastogenesis by Producing RANKL in Response to BMP2

Chondrocytes express RANKL, but their role in osteoclastogenesis is not clear. We report that hypertrophic chondrocytes induce osteoclast formation through RANKL production stimulated by BMP2 and Runx2/Smad1 and thus they may regulate resorption of calcified matrix by osteoclasts at growth plates.
Designing technology for community appropriation

INTRODUCTION Computation is weaving itself into our daily fabric. All around us new devices, environments, and systems are opening themselves up for user adoption and in many cases adaptation, such as SMS messaging on cell phones. New and unexpected interactions with the immaterial have expanded the design territory to include people as designers, and Moran described design as a “negotiated social process”. [7] The design process now extends beyond the formulation of a computational artifact and onto how the user experiences an artifact. This mutual relationship has heightened the understanding of what we, as designers, should be designing for, but does not necessarily provide us with the tools and practices to design technology that is truly open for later appropriation. This seems even more challenging in the case of technology, which is specifically aimed at a whole community of users rather than an individual [5].
Intermodulation Distortions of Bang–Bang Control Class D Amplifiers

Audio Class D amplifiers (CDAs) based on bang-bang architecture are arguably the most power efficient architecture of all CDAs reported to-date due to their sheer hardware simplicity. At this juncture, the intermodulation distortion (IMD), an imperative measure that defines fidelity, remains unreported for bang-bang CDAs. In this paper, we investigate the mechanisms of and circuit parameters affecting the IMD of bang-bang CDAs. An interesting phenomenon is that the even-order IMD which is usually negligible/unreported in other CDAs and linear amplifiers may be higher than odd-order IMD therein. We derive analytical expressions for the IMD of the ideal and practical bang-bang CDAs, and the derived expressions are verified against HSPICE simulations and on the basis of measurements on a physical bang-bang CDA. These derived analytical expressions are useful as they provide valuable insights to the design of bang-bang CDAs and how parameters can be tradedoff to improve the IMD.
Immunochemical characterization of Aspergillus fumigatus antigens

Culture filtrate antigens of Aspergillus fumigatus Ag 534 were purified by preparative isoelectric focusing and affinity chromatography. One of the pooled antigen fractions from the preparative isoelectric focusing step (pool 2) was passed through a concanavalin A column and yielded two components, designated antigens IIa and IIb. Antigen IIb reacted more strongly than antigen IIa with all of the aspergilloma and allergic bronchopulmonary aspergillosis sera tested by enzyme-linked immunosorbent assay. The glycoprotein nature of antigen IIb was shown by the concanavalin A binding properties and staining reactions of the components.
KIF21A novel deletion and recurrent mutation in patients with congenital fibrosis of the extraocular muscles-1.

Kinesin family member 21A (KIF21A) mutation is the most common cause for congenital fibrosis of the extraocular muscles type 1 (CFEOM1) in populations worldwide. However, only 12 missense mutations have been reported to date. In this study, KIF21A screening was performed in two Chinese families with CFEOM1. Ophthalmological examinations were performed. The coding exons and adjacent intronic regions of KIF21A were analyzed with cycle sequencing. The novel mutation identified was further evaluated in 150 normal control individuals and available family members. Two heterozygous mutations in KIF21A, c. 3000_3002delTGA (p.Asp1001del) and c.2861G>A (p.Arg954Gln), were detected in two families. The novel deletion involves a conserved residue in the coiled-coil domain of KIF21A and is co-segregated with the disease in the examined family, yet was absent in the 300 control chromosomes. In addition, apart from typical phenotypes for CFEOM1, optic disc hypoplasia was also observed in two patients. Deletion mutation in KIF21A has not been previously reported. Our study expands the KIF21 mutation spectrum. This study adds to the current state of knowledge about KIF21A mutations and CFEOM1, which may improve future clinical practice.
Ultrastructural localization of glycogen phosphorylase predominantly in astrocytes of the gerbil brain

The localization of glycogen phosphorylase in gerbil brain was determined by immunoelectron microscopy using the pre‐embedding peroxidase technique. Electron‐dense granular reaction product of peroxidase activity was observed in astrocytes of all brain regions examined (cerebral cortex, striatum, cerebellar cortex, hippocampal formation, corpus callosum, mesencephalic trigeminal nucleus). The reaction product was distributed in a diffuse pattern throughout the cytoplasmic matrix of perikarya and processes; sometimes the nucleus of labeled astrocytes also contains immunopositive material. The light microscopically visible glycogen phosphorylase immunoreactivity associated with capillaries could be characterized as a staining of astrocytic endfeet ensheathing capillaries. Endothelial cells and pericytes were never labeled. In addition to astrocytes, ependymal cells also presented immunopositive material in their cytoplasm. On the other hand, no reaction product was observed in cells identified as oligodendroglia or microglia. Neurons (with the exception of neurons of the mesencephalic trigeminal nucleus), their processes, and their synaptic endings were free of reaction product. In the neuropil we frequently observed immunopositive glial processes adjacent to synaptic structures. This intimate spatial relationship may be interpreted as a morphological sign of a metabolic interaction. The data support the hypothesis that astroglia play a key role in glycogen metabolism and energization of the brain. © 1996 Wiley‐Liss, Inc.