Miracle-dz/UniFAR-FTUs huggingface dataset:
THE MINIMUM VITAMIN REQUIREMENTS OF THE L AND HELA CELLS IN TISSUE CULTURE, THE PRODUCTION OF SPECIFIC VITAMIN DEFICIENCIES, AND THEIR CURE

Seven vitamins have to date proved essential for the survival and multiplication of a mouse fibroblast (strain L) and a human carcinoma cell (strain HeLa) in tissue culture: choline, folic acid, nicotinamide, pantothenic acid, pyridoxal, riboflavin, and thiamin. It was necessary to cultivate the cells for 5 to 15 days in a medium lacking the specific vitamin before the deficiency became apparent in the cessation of multiplication and the development of specific cytopathogenic effects. In their early stages these changes could be reversed by the addition of the missing vitamin, an in vitro, "cure" of a vitamin deficiency. The maximally effective concentrations were in the range 107 to 108 gm. per ml. The probability that additional vitamins not demonstrably essential under the conditions of the present experiments are nevertheless required for survival and growth is discussed in the text.
Exploring chainsaw operator occupational exposure to carbon monoxide in forestry

ABSTRACT Exposure to carbon monoxide (CO) emitted by chainsaws can negatively impact health in forestry workers. This exploratory study measures CO concentration within the breathing zone of chainsaw operators during motor-manual operations, and discusses the potential influences on CO exposure levels. A CO monitoring instrument was paired with a concurrent video recording of task activities to enable correlation of exact working operations to critical exposure levels. Multiple streams of meteorological data were also collected from sensors worn by the eight professional tree fellers/log makers. Time-weighted averages were applied to investigate levels of CO exposure during a nominal 1-hr monitoring period. The differing task demands and environment were found to influence worker exposure to CO, supporting previous research. Pending further investigation, a number of possible actions are recommended to reduce observed high exposure levels and/or emission concentration.
Ethnographic Study of Transition: On the Threshold of Adult Life.

This monograph reports on an ethnographic study of the transition from school to work of 12 individuals with mental retardation. Throughout the book, examples from the lives of these individuals are used to illustrate findings and principles. The first chapter describes the methods of data collection and analysis, which focused on the relation between critical life events and the tr.snsitiqz frcm --h2D1 tr ad,,,It life. Chanter 2 nresents holistic, person-centered data about the 12 individuals, focusing on the pathways taken, the personal support systems developed, critical life events, expectations of others, and the individuals' own wishes and values. Chapter 3 introduces five skills necessary for successful mentorship in transition, including: (1) problem solving, (2) technical career planning, (3) ability to collect and assess life history information, (4) ability to analyze an individual's adult script and social network, and (5) ability to assess the individual's mediative capacities. Chapter 4 shows how these factors play out in actual lives, in discussion and evaluation of eight life histories. Stressed throughout is the importance of individual differences in planning and implementing transition goals. (Contains 65 references.) (DB) *********************************************************************** Reproductions supplied by EDRS are the best that can be made from the original document. ***********************************************************************
A novel higher order cyclic cumulant based direction finding algorithm in multipath

We develop a novel higher order cyclic cumulant based direction finding algorithm in multipath. Firstly, three fourth-order cyclic cumulant matrices of the received signals are constructed and the generalized steering vector is estimated, then forward-backward linear prediction (FBLP) method with subarrays is used to resolve the DOAs of each group of coherent signals of interest (SOIs), which can improve resolution of closely spaced coherent signals in small data length. The simulations show the advantages of the proposed method compared with the EVESPA algorithm. Unlike EVESPA algorithm, the total number of signals (SOIs and interferences) impinging on the array can be more than 2K(K-1)/3 with K-sensor uniform linear subarray and a maximum of 2K(K-1)/3 coherent SOIs can be resolved. The capability of noise-suppression and signal-selectivity in multipath environment of mobile communications is improved.
Recommendation of Nasogastric Tube Removal in Acute Stroke Patients Based on Videofluoroscopic Swallow Study

Objective To evaluate the safety of nasogastric tube (NGT) removal and change to oral feeding with a food thickener for acute stroke patients in whom a videofluoroscopic swallow study (VFSS) confirmed thin liquid aspiration. Methods We retrospectively examined data of 199 patients with first stroke who were diagnosed with dysphagia from 2011 to 2015. Swallowing function was evaluated using VFSS. Patients included in this study were monitored for 4 weeks to identify the occurrence of aspiration pneumonia. The penetration-aspiration scale (PAS) was used to assess VFSS findings. The patients were divided into thin-liquid aspiration group (group 1, n=104) and no thin-liquid aspiration group (group 2, n=95). Results The feeding method was changed from NGT feeding to oral feeding with food thickener (group 1) and without food thickener (group 2). The PAS scores of thin and thick liquids were 6.46±0.65 and 1.92±0.73, respectively, in group 1 and 2.65±0.74 and 1.53±0.58, respectively, in group 2. Aspiration pneumonia developed in 1.9% of group 1 and 3.2% of group 2 (p=0.578), with no significant difference between the groups. Conclusion We concluded that removing the NGT and changing to oral feeding with a food thickener is a safe food modification for acute stroke patients with thin liquid aspiration. Therefore, we recommend that VFSS should be conducted promptly in acute stroke patients to avoid unnecessary prolonged NGT feeding.
The personal and the professional: Buddhist practice and systemic therapists

This dissertation consists of two articles. The first article presents a literature review of research on therapists’ personal and professional lives as practitioners of Buddhist meditation and psychotherapy in the past ten years. Nineteen articles were reviewed that met the inclusion criteria of a) between 2004 and 2014; b) exploring meditation; and c) studies related to therapist personal or professional lives. The results of the review identified four broad themes: a) presence and acceptance; b) empathy; c) countertransference; and d) self-care/compassion and gratitude. The content analysis indicated a positive association between therapist meditation practice and therapist qualities, both for personal development and enhanced clinical competence. The second article describes a qualitative research study to discover the embodied, lived experiences of practicing therapists as they move between their personal study of Buddhist meditation practices and their professional clinical work. In-depth semi-structured interview data were analyzed, revealing two superordinate themes: a) a way of seeing and being in the world; and b) the personal and the professional in the therapy room. The research findings have clinical implications in gaining an understanding of on-going personal and professional development for experienced therapists and contributing to the literature on professional competence, in particular, therapeutic presence, acceptance, empathy, compassion, and practitioner well-being. The electronic version of this dissertation is available in the open-access OhioLink ETD Center, www.ohiolink. edu/etd. Keywords: Buddhist meditation; marriage and family therapy; systemic psychotherapy; therapist development; compassionate therapeutic presence; personal; and professional BUDDHIST PRACTICE AND SYSTEMIC THERAPISTS ii
Identification of patient‐specific peptides for detection of M‐proteins and myeloma cells

We have taken advantage of the selection power of phage display technology to define specific peptide mimotopes that recognize individual M‐proteins, isolated from patients with multiple myeloma. Preferred amino acid motifs of phages binding to M‐proteins were identified in 6/9 patients investigated. Chemically synthesized peptides, corresponding to the phage‐displayed peptide inserts, were used to verify the specificity of binding in competition assays. The peptides were able to bind to the M‐proteins, as well as the myeloma cells, with high sensitivity and specificity. Employing simple immunological techniques, < 0.01 g/l of M‐protein could be quantified, suggesting a novel way for monitoring minimal residual disease in the production of guidelines for adjusting or reintroducing conventional chemotherapy. The peptide mimotopes defined by this technology may be useful as tumour‐specific targeting agents and as a tool for purging cells in autologous bone marrow transplantation.
Psychosocial adaptation of middle childhood boys with hypospadias after genital surgery.

OBJECTIVE To compare the psychosocial adaptation of boys with hypospadias after genital surgery to a community sample. METHODS Boys (6 to 10 years) with a history of hypospadias repair (n = 175) were compared with a community sample (n = 333) in a postal questionnaire survey using the Child Behavior Checklist. RESULTS Few significant differences between cases and controls emerged. Boys with hypospadias were (slightly) lower in social involvement but did not perform more poorly in school. Boys with hypospadias displayed fewer externalizing behavior problems than controls, but a significant difference in nocturnal enuresis was not detected. Level of behavior problems did not differentiate hypospadias severity subgroups, but greater surgical and hospitalization experiences were associated with increased internalizing problems. Poorer cosmetic appearance of the genitals was associated with worse school performance. CONCLUSIONS Surgically corrected hypospadias should not be considered a risk factor for poor psychosocial adaptation in childhood, but emotional problems increase with the number of hospital-related experiences.
Macrophage Receptor with Collagenous Structure (MARCO) Is Processed by either Macropinocytosis or Endocytosis-Autophagy Pathway

The Macrophage Receptor with COllagenous structure (MARCO) protein is a plasma membrane receptor for un-opsonized or environmental particles on phagocytic cells. Here, we show that MARCO was internalized either by ruffling of plasma membrane followed by macropinocytosis or by endocytosis followed by fusion with autophagosome in CHO-K1 cells stably transfected with GFP-MARCO. The macropinocytic process generated large vesicles when the plasma membrane subsided. The endocytosis/autophagosome (amphisome) generated small fluorescent puncta which were visible in the presence of glutamine, chloroquine, bafilomycin, ammonia, and other amines. The small puncta, but not the large vesicles, co-localized with LC3B and lysosomes. The LC3-II/LC3-I ratio increased in the presence of glutamine, ammonia, and chloroquine in various cells. The small puncta trafficked between the peri-nuclear region and the distal ends of cells back and forth at rates of up to 2–3 μm/sec; tubulin, but not actin, regulated the trafficking of the small puncta. Besides phagocytosis MARCO, an adhesive plasma membrane receptor, may play a role in incorporation of various extracellular materials into the cell via both macropinocytic and endocytic pathways.
Localization of the human NCAM gene to band q23 of chromosome 11: the third gene coding for a cell interaction molecule mapped to the distal portion of the long arm of chromosome 11

cDNA clones containing sequences coding for the murine neural cell adhesion molecule (N-CAM) were used in Southern hybridizations on human genomic DNA and demonstrated approximately 90% homology between human and murine NCAM genes. In situ hybridization with one of these clones was performed on human metaphase chromosomes and allowed the localization of the human NCAM gene to band q23 of chromosome 11. The genes for two other cell surface molecules believed to be involved in cell-cell interactions, Thy-1 and the delta chain of the T3-T cell receptor complex, have recently been localized to the same region of chromosome 11 in man. Moreover, this region of the human chromosome 11 appears to be syntenic to a region of murine chromosome 9 that also contains the staggerer locus: staggerer mice show abnormal neurological features which may be related to abnormalities in the conversion of the embryonic to the adult forms of the N-CAM molecule.
Adding fuzzy color information for image classification

Color is a powerful feature for image analysis but it is usually not used in image classification schemes. We propose a method to combine fuzzy color information with the result obtained from a One-Versus-All classifier (OVA) trained with Bag-of-features. This method consists in weighting the outputs of the OVA classifier based on the distances between the new image to be classified and the classes. Experimental results show that our approach improves OVA classifier performance.
Stacksplorer: call graph navigation helps increasing code maintenance efficiency

We present Stacksplorer, a new tool to support source code navigation and comprehension. Stacksplorer computes the call graph of a given piece of code, visualizes relevant parts of it, and allows developers to interactively traverse it. This augments the traditional code editor by offering an additional layer of navigation. Stacksplorer is particularly useful to understand and edit unknown source code because branches of the call graph can be explored and backtracked easily. Visualizing the callers of a method reduces the risk of introducing unintended side effects. In a quantitative study, programmers using Stacksplorer performed three of four software maintenance tasks significantly faster and with higher success rates, and Stacksplorer received a System Usability Scale rating of 85.4 from participants.
Prediction and Stability of Reading Problems in Middle Childhood

The longitudinal prediction of reading problems from fourth grade to sixth grade was investigated with a sample of 173 students. Reading problems at the end of sixth grade were defined by significantly below average performance (≤ 15th percentile) on reading factors defining word reading, fluency, and reading comprehension. Sixth grade poor reader status was predicted by fall of fourth grade passage reading fluency, spelling fluency, and the number of reading problems identified by teachers. Reading fluency and spelling fluency were significant predictors in logistic regression equation that combined to yield a screening battery with an area under the curve of . 91. These results suggest that brief assessments of reading and spelling fluency in fourth grade may be able to identify students in middle childhood who have a reading problem or who are at risk for experiencing reading problems in sixth grade.
Lossy compression of packet classifiers

Packet classification is a building block in many network services such as routing, filtering, intrusion detection, accounting, monitoring, load-balancing and policy enforcement. Compression has gained attention recently as a way to deal with the expected increase of classifiers size. Typically, compression schemes try to reduce a classifier size while keeping it semantically-equivalent to its original form. Inspired by the advantages of popular compression schemes (e.g. JPEG and MPEG), we study in this paper the applicability of lossy compression to create packet classifiers requiring less memory than optimal semantically-equivalent representations. Our objective is to find a limited-size classifier that can correctly classify a high portion of the traffic so that it can be implemented in commodity switches with classification modules of a given size. We develop optimal dynamic programming based algorithms for several versions of the problem and describe how a small amount of traffic that cannot be classified can be easily treated, especially in software-defined networks. We generalize our solutions for a wide range of classifiers with different similarity metrics. We evaluate their performance on real classifiers and traffic traces and show that in some cases we can reduce a classifier size by orders of magnitude while still classifying almost all traffic correctly.
Detecting Pathway Cross-Talks by Analyzing Conserved Functional Modules across Multiple Phenotype-Expressing Organisms

Biological systems are organized hierarchically, starting from the protein level and expanding to pathway or even higher levels. Understanding interactions at lower levels (proteins interactions) in the hierarchy will help us understand interactions at higher levels (pathway cross-talks). Identifying cross-talks that are related to the expression of a particular phenotype will be of interest to genetic engineers, because it will provide information on how different cellular subsystems could work together to express a phenotype. Current research has typically focused on identifying genotype-phenotype associations or pathway-phenotype associations. In contrast, we developed a method to identify phenotype-related pathway cross talks by obtaining conserved groups of interacting proteins (functional modules). By applying our method to two groups of hydrogen producing organisms (light fermentation and dark fermentation), we have shown that our method effectively unearths known pathway cross-talks that are important to hydrogen production.
Genetic analysis of selenocysteine biosynthesis in the archaeon Methanococcus maripaludis

In Archaea selenocysteine (Sec) is synthesized in three steps. First seryl‐tRNA synthetase acylates tRNASec with serine to generate Ser‐tRNASec. Then phosphoseryl‐tRNASec kinase (PSTK) forms Sep‐tRNASec, which is converted to Sec‐tRNASec by Sep‐tRNA:Sec‐tRNA synthase (SepSecS) in the presence of selenophosphate produced by selenophosphate synthetase (SelD). A complete in vivo analysis of the archaeal Sec biosynthesis pathway is still unavailable, and the existence of a redundant pathway or of a rescue mechanism based on the conversion of Sep‐tRNASec to Cys‐tRNASec during selenium starvation, cannot be excluded. Here we present a mutational analysis of Sec biosynthesis in Methanococcus maripaludis strain Mm900. Sec formation is abolished upon individually deleting the genes encoding SelD, PSTK or SepSecS; the resulting mutant strains could no longer grow on formate while growth with H2 + CO2 remained unaffected. However, deletion of the PSTK and SepSecS genes was not possible unless the selenium‐free [NiFe]‐hydrogenases Frc and Vhc were expressed. This required the prior deletion of either the gene encoding SelD or that of HrsM, a LysR‐type regulator suppressing transcription of the frc and vhc operons in the presence of selenium. These results show that M. maripaludis Mm900 is facultatively selenium‐dependent with a single pathway of Sec‐tRNASec formation.
Surface Plasmon Resonance Studies Resolve the Enigmatic Endotoxin Neutralizing Activity of Polymyxin B*

Polymyxin B (PMB), a cyclic cationic peptide antibiotic, despite its severe side effects continues to occupy a premiere position for treating endotoxicosis. Its mode of neutralization of endotoxin has remained elusive for the last three decades. Several synthetic peptide mimics of PMB, capable of binding endotoxin, have been made. However, the binding ability alone appears to be a deceptive indicator of endotoxin neutralizing activity as molecules with similar binding propensities could either sequester or opsonize the toxin. Hence identification of additional physical parameters which describe adequately the outcome of PMB-endotoxin interaction become imperative. Surface plasmon resonance (SPR) studies reported here show that several mimics of PMB despite exhibiting lipopolysaccharide binding affinities comparable with it but, unlike it, do not sequester the endotoxin. These studies thus provide a striking illustration of the difference in the behavior of PMB,vis a vis its mimics toward the endotoxin lamellae, and define further, in chemical terms, mechanism of the action of PMB and allow us to posit that the design of molecules as effective antidotes for sepsis should incorporate the ability to sequester endotoxin specifically.
Boosting stochastic problem solvers through online self-analysis of performance

In many combinatorial domains, simple stochastic algorithms often exhibit superior performance when compared to highly customized approaches. Many of these simple algorithms outperform more sophisticated approaches on difficult benchmark problems; and often lead to better solutions as the algorithms are taken out of the world of benchmarks and into the real-world. Simple stochastic algorithms are often robust, scalable problem solvers. This thesis explores methods for combining sets of heuristics within a single stochastic search. The ability of stochastic search to amplify heuristics is often a key factor in its success. Heuristics are not, however, infallible and in most domains no single heuristic dominates. It is therefore desirable to gain the collective power of a set of heuristics; and to design a search control framework capable of producing a hybrid algorithm from component heuristics with the ability to customize itself to a given problem instance. A primary goal is to explore what can be learned from quality distributions of iterative stochastic search in combinatorial optimization domains; and to exploit models of quality distributions to enhance the performance of stochastic problem solvers. We hypothesize that models of solution quality can lead to effective search control mechanisms, providing a general framework for combining multiple heuristics into an enhanced decision-making process. These goals lead to the development of a search control framework, called QD-BEACON, that uses online-generated statistical models of search performance to effectively combine search heuristics. A prerequisite goal is to develop a suitable stochastic sampling algorithm for combinatorial search problems. This goal leads to the development of an algorithm called VBSS that makes better use, in general, of the discriminatory power of a given search heuristic as compared to existing sampling approaches. The search frameworks of this thesis are evaluated on combinatorial optimization problems. Specifically, we show that: (1) VBSS is an effective method for amplifying heuristic performance for the weighted tardiness sequencing problem with sequence-dependent setups; (2) QD-BEACON can enhance the current best known algorithm for weighted tardiness sequencing; and (3) QD-BEACON and VBSS together provide the new best heuristic algorithm for the constrained optimization problem known as RCPSP/max.
Performance Anomaly Detection and Bottleneck Identification

In order to meet stringent performance requirements, system administrators must effectively detect undesirable performance behaviours, identify potential root causes, and take adequate corrective measures. The problem of uncovering and understanding performance anomalies and their causes (bottlenecks) in different system and application domains is well studied. In order to assess progress, research trends, and identify open challenges, we have reviewed major contributions in the area and present our findings in this survey. Our approach provides an overview of anomaly detection and bottleneck identification research as it relates to the performance of computing systems. By identifying fundamental elements of the problem, we are able to categorize existing solutions based on multiple factors such as the detection goals, nature of applications and systems, system observability, and detection methods.