Miracle-dz/UniFAR-FTUs huggingface dataset:
Development of Hands-on IP Network Practice System with Automatic Scoring Function

The purpose of this study is to give students hands-on practical learning for IP network skills. We have been developing the hands-on IP network practice system using virtual machines. Students can practice IP network skills freely at anytime on just one general-purpose PC. We have implemented this system by applying the virtual Linux environment User Mode Linux. In this research, we developed two new functions for this system. First, we developed the Automatic Scoring Function. This function compares student's configuration files and teacher's configuration files. Then, this function can show a result as a percentage of correct answer. In addition, this function can display the details of items that are incorrect. Using this function, students are able to find that their network configurations are correct or incorrect by themselves. Students are able to do self-service training and testing all in one place. Second, we developed the Assignment Making Support Function. Teachers are able to make assignments by using this function easily. To evaluate new functions, this system was experimented. The results indicated that this system had sufficient performance for introductory network courses for undergraduate students.
A Global-Model Naive Bayes Approach to the Hierarchical Prediction of Protein Functions

In this paper we propose a new global--model approach for hierarchical classification, where a single global classification model is built by considering all the classes in the hierarchy -- rather than building a number of local classification models as it is more usual in hierarchical classification. The method is an extension of the flat classification algorithm naive Bayes. We present the extension made to the original algorithm as well as its evaluation on eight protein function hierarchical classification datasets. The achieved results are positive and show that the proposed global model is better than using a local model approach.
Formal Vulnerability Analysis of a Security System for Remote Fieldbus Access

As fieldbus networks are becoming accessible from the Internet, security mechanisms to grant access only to authorized users and to protect data are becoming essential. This paper proposes a formally based approach to the analysis of such systems, both at the security protocols level and at the system architecture level. This multilevel analysis allows the evaluation of the effects of an attack on the overall system, due to security problems that affect the underlying security protocols. A case study on a typical fieldbus security system validates the approach.
‘Hip to be Irish’: ethnicity and Bourdieu's ‘Forms of Capital’

ABSTRACT A ‘new Irish’ American ethnicity surfaced in the 1980s – according to a number of scholars and journalists – and is comprised of what some consider to be more ‘authentic’ and ‘traditional’ Irish cultural attributes. Defining authentic and traditional Irishness is complicated, however, by the recent influx and highly visible forms of economic capital and media attention stemming from the Celtic Tiger – the surge of economic prosperity that began in Ireland in the 1990s – and the commercializing of Irish music and dance – such as Riverdance. In this paper, I propose, through my study involving Irish language enthusiasts, that this ‘new’ form of Irish ethnicity is more the result of rather than the reason for this surge in popularity. I use Pierre Bourdieu's ‘Forms of Capital’ in questioning the ‘new Irish’ American ethnicity, arguing instead that a deeply seated, less-visible reservoir of cultural and social capital form the basis for this perceived contemporary ethnicity.
Field philosophy: environmental learning and moral development in Isle Royale National Park

We conducted a qualitative analysis of 5 years of student writing data to understand learning and moral development on a field philosophy course in Isle Royale National Park. We were interested in the connection between physical experiences in the natural world and the way students care about or value nonhuman beings, natural systems, and place. Students consistently demonstrated a shift from a dualistic to a more complex understanding of ideas, relationships, and the natural world. This widening perception and moral awareness occurred in tandem with course content learning. In this paper we describe the model that emerged from our data to describe this shift from dualism to complexity – from self-awareness, to social learning, to curriculum engagement, to a wider moral community – and the factors that facilitate each step. We also provide a planning and assessment tool to help other educators begin to observe ethical development and relational growth in their own interdisciplinary environmental field classes.
Modulation of synaptic GABAA receptor function by zolpidem in substantia nigra pars reticulata

AbstractAim:The substantia nigra pars reticulata (SNr) constitutes one of the output centers of the basal ganglia, and its abnormal activity is believed to contribute to some basal ganglia motor disorders. Different lines of evidence revealed a major contribution of GABAA receptor-mediated synaptic inhibition in controlling the activity of SNr. The benzodiazepine binding site within the GABAA receptor is a modulation site of significant clinical interest. A high density of benzodiazepine binding sites has been reported in the rat SNr. In the present study, we investigate the effects of activating benzodiazepine binding sites in the SNr.Methods:Whole-cell patch-clamp recordings and motor behavior were applied.Results:Superfusion of zolpidem, a benzodiazepine binding agonist, at 100 nmol/L significantly prolonged the decay time of GABAA receptor-mediated postsynaptic currents. The prolongation on decay time induced by zolpidem was sensitive to the benzodiazepine antagonist flumazenil, confirming the specificity on the benzodiazepine site. Zolpidem at 1 μmol/L exerted a stronger prolongation on the decay time. A further experiment was performed on behaving rats. A unilateral microinjection of zolpidem into the rat SNr caused a robust contralateral rotation, which was significantly different from that of control animals receiving the vehicle injection.Conclusion:The present in vitro and in vivo findings that zolpidem significantly potentiated GABA currents and thus inhibited the activity of the SNr provide a rationale for further investigations into its potential in the treatment of basal ganglia disorders.
A Comparative Study of Lectin Affinity Based Plant N-Glycoproteome Profiling Using Tomato Fruit as a Model*

Lectin affinity chromatography (LAC) can provide a valuable front-end enrichment strategy for the study of N-glycoproteins and has been used to characterize a broad range eukaryotic N-glycoproteomes. Moreover, studies with mammalian systems have suggested that the use of multiple lectins with different affinities can be particularly effective. A multi-lectin approach has also been reported to provide a significant benefit for the analysis of plant N-glycoproteins; however, it has yet to be determined whether certain lectins, or combinations of lectins are optimal for plant N-glycoproteome profiling; or whether specific lectins show preferential association with particular N-glycosylation sites or N-glycan structures. We describe here a comparative study of three mannose-binding lectins, concanavalin A, snowdrop lectin, and lentil lectin, to profile the N-glycoproteome of mature green stage tomato (Solanum lycopersicum) fruit pericarp. Through coupling lectin affinity chromatography with a shotgun proteomics strategy, we identified 448 putative N-glycoproteins, whereas a parallel lectin affinity chromatography plus hydrophilic interaction chromatography analysis revealed 318 putative N-glycosylation sites on 230 N-glycoproteins, of which 100 overlapped with the shotgun analysis, as well as 17 N-glycan structures. The use of multiple lectins substantially increased N-glycoproteome coverage and although there were no discernible differences in the structures of N-glycans, or the charge, isoelectric point (pI) or hydrophobicity of the glycopeptides that differentially bound to each lectin, differences were observed in the amino acid frequency at the −1 and +1 subsites of the N-glycosylation sites. We also demonstrated an alternative and complementary in planta recombinant expression strategy, followed by affinity MS analysis, to identify the putative N-glycan structures of glycoproteins whose abundance is too low to be readily determined by a shotgun approach, and/or combined with deglycosylation for predicted deamidated sites, using a xyloglucan-specific endoglucanase inhibitor protein as an example.
Transcriptional and post-translational regulation of CYP1A1 by primaquine.

Regulation of the CYP1A1 gene has been shown to involve the aryl hydrocarbon receptor and the CYP1A1 gene expression is induced by AhR ligands. Primaquine is an antimalarial agent that does not exhibit the structural properties of a classical AhR ligand. We have evaluated the mechanisms by which this compound induces CYP1A1 expression using rat hepatoma H4IIE cells and V79 cells stably expressing CYP1A1. In H4IIE cells, primaquine caused a time- and dose-dependent increase of CYP1A1 mRNA and protein expression. The transcriptional activation of the CYP1A1 gene by primaquine was strictly XRE-dependent, as shown by transfection of different CYP1A1 pGL3 reporter constructs in H4IIE cells, and the involvement of the AhR was shown by activation of a Gal4-AhR hybrid protein by primaquine in transfected cells. Furthermore, primaquine caused transformation of the cytosolic AhR to a DNA-binding form, in vitro, suggesting that primaquine directly activates the receptor complex. In addition to its action at the transcriptional level, primaquine caused a dose-dependent inhibition of CYP1A1 degradation with an IC(50) of 3.3 , as seen in mammalian V79 cells. This was not due to the lysosomotropic activity of the drug since other lysosomotropic agents were ineffective. Primaquine formed a type II binding spectrum with CYP1A1 and inhibited the CYP1A1-dependent ethoxyresorufin O-deethylase activity in vitro with a K(i) of 1.3 microM, which is close to the IC(50), suggesting that the drug protects CYP1A1 from degradation by binding at the active site. It is concluded that CYP1A1 is regulated by primaquine both on the transcriptional as well as on a post-translational level.
Educational leadership effectiveness: a Rasch analysis

Purpose – The purposes of this paper are, first, to establish the psychometric properties of the ELP tool, and, second, to test, using a Rasch item response theory analysis, the hypothesized progression of challenge presented by the items included in the tool. Design/methodology/approach – Data were collected at two time points through a survey of the educational leadership practices of school principals (n=148) and their teachers (n=5,425). The survey comprised seven effectiveness scales relating to school-wide dimensions of leadership, and one scale relating to the effectiveness of individual principals’ leadership. The authors undertook validation of the hypothesized structure of the eight ELP scales using the Rasch rating scale model. Findings – The authors established constructs that underpin leadership practices that are more and less effectively performed and determined the nature of their progression from those that are relatively routine through those that are more rigorous and challenging to ena.
Cardiolipin Prevents Membrane Translocation and Permeabilization by Daptomycin*

Background: Daptomycin forms oligomeric pores in bacterial cell membranes. Cardiolipin is a membrane lipid associated with bacterial resistance to the antibiotic. Results: Cardiolipin makes liposomes impervious to daptomycin permeabilization, and it confines daptomycin to the outer membrane leaflet. Conclusion: Preventing daptomycin from reaching the inner membrane leaflet inhibits pore formation. Significance: Bacteria may become resistant to daptomycin by changing their membrane lipid composition. Daptomycin is an acidic lipopeptide antibiotic that, in the presence of calcium, forms oligomeric pores on membranes containing phosphatidylglycerol. It is clinically used against various Gram-positive bacteria such as Staphylococcus aureus and Enterococcus species. Genetic studies have indicated that an increased content of cardiolipin in the bacterial membrane may contribute to bacterial resistance against the drug. Here, we used a liposome model to demonstrate that cardiolipin directly inhibits membrane permeabilization by daptomycin. When cardiolipin is added at molar fractions of 10 or 20% to membranes containing phosphatidylglycerol, daptomycin no longer forms pores or translocates to the inner membrane leaflet. Under the same conditions, daptomycin continues to form oligomers; however, these oligomers contain only close to four subunits, which is approximately half as many as observed on membranes without cardiolipin. The collective findings lead us to propose that a daptomycin pore consists of two aligned tetramers in opposite leaflets and that cardiolipin prevents the translocation of tetramers to the inner leaflet, thereby forestalling the formation of complete, octameric pores. Our findings suggest a possible mechanism by which cardiolipin may mediate resistance to daptomycin, and they provide new insights into the action mode of this important antibiotic.
Clinical correlations of serial quantitative blood cultures determined by lysis-centrifugation in patients with persistent septicemia

The potential clinical value of colony counts determined by the lysis-centrifugation blood culture method was studied by reviewing the records of eight patients with persistent septicemia in whom colony counts were available on at least 3 days. Colony counts of the five patients who survived decreased steadily as the patients improved. One of the three patients who died had counts repeatedly below 1.0 CFU/ml while she was clinically stable and higher counts when her condition deteriorated. Two patients died despite decreasing colony counts. One was improving and died unexpectedly of an unrelated cause; the other died of candidiasis, but declining serial arabinitol/creatinine ratios suggested a partial response to therapy. In addition, septicemia related to infected intravenous catheters was documented by demonstrating large differences in colony counts determined simultaneously from two different sites in two patients and by demonstrating a precipitous drop in CFU per milliliter after removal of the infected catheter in one patient. Routine availability of colony counts appears to be an important advantage of the lysis-centrifugation method.
Measuring Presence to Enhance the Spectators' Participation on the Moving Images using EEG, IDQS and PQS

While presence is a subjective experience, it is based on a perceptive reaction to the stimulation from the image. This paper suggests the measuring and analyzing method of individual emotion and participation in terms of presence on the moving images. The measuring presence was carried out analyzing electroencephalography (EEG) signal, individual differences questionnaire survey (IDQS) and presence questionnaire survey (PQS). In the EEG test, the left hemisphere's value in the brain was high. Over 90 percent of the participants' IDQS and PQS were matched with the EEG test results. From the result, we found that the participants mostly felt pleasant, involved and joy. The results of this survey are useful to enhance spectators' presence.
Race, Space, and Being Policed

Research on race and policing has consistently documented the link between race, space, and policing with racial disparities in perceptions and experiences often explained by nature of policing within low-income, distressed communities of color. This study adds a new dimension to past research by examining the relationship between race, racial composition of place, and residents’ experiences with southern police across public and semipublic spaces within a middle-class, low-crime, tourism-driven resort area. Using grounded theory methods, this study combines survey data with in-depth group interviews and structured observations to reveal variability in participants’ experiences with southern patrols. Specifically, African American participants expressed concern for excessive police distribution, differential enforcement, and social exclusion, offering the least supportive attitudes regarding officer demeanor. These findings highlight the importance of using comprehensive measures to access residents’ experiences with police and contribute to the understanding of how various geographic contexts might produce racial disparities, highlighting the salience of race in the policing of ethnically diverse public spaces.
Coordination through mutual notification in cooperative multiagent reinforcement learning

We present a new algorithm for cooperative reinforcement learning in multiagent systems. Our main concern is the correct coordination between the members of the team: We seek to obtain an optimal solution for the team as a whole while keeping the learning as much decentralized as possible. We consider autonomous and independently learning agents that do not store any explicit information about their teammatesý behavior, as well as possibly different reward functions for each agent. Coordination between agents occurs through communication, namely the mutual notification algorithm.
Learning in Games via Reinforcement and Regularization

We investigate a class of reinforcement learning dynamics where players adjust their strategies based on their actions' cumulative payoffs over time-specifically, by playing mixed strategies that maximize their expected cumulative payoff minus a regularization term. A widely studied example is exponential reinforcement learning, a process induced by an entropic regularization term which leads mixed strategies to evolve according to the replicator dynamics. However, in contrast to the class of regularization functions used to define smooth best responses in models of stochastic fictitious play, the functions used in this paper need not be infinitely steep at the boundary of the simplex; in fact, dropping this requirement gives rise to an important dichotomy between steep and nonsteep cases. In this general framework, we extend several properties of exponential learning, including the elimination of dominated strategies, the asymptotic stability of strict Nash equilibria, and the convergence of time-averaged trajectories in zero-sum games with an interior Nash equilibrium.
Base force/torque sensing for position based Cartesian impedance control

In this paper, a position based impedance controller (i.e. admittance controller) is designed by utilizing measurements of a force/torque sensor, which is mounted at the robot's base. In contrast to conventional force/torque sensing at the end-effector, placing the sensor at the base allows to implement a compliant behavior of the robot not only with respect to forces acting on the end-effector but also with respect to forces acting on the robot's structure. The resulting control problem is first analyzed in detail for the simplified one-degree-of-freedom case in terms of stability and passivity. Then, an extension to the Cartesian admittance control of a robot manipulator is discussed. Furthermore, it is shown how the steady state properties of the underlying position controller can be taken into account in the design of the outer admittance controller. Finally, a simulation study of the Cartesian admittance controller applied to a three-degrees-of-freedom manipulator is presented.
Fluid Flow Shear Stress Stimulation on a Multiplex Microfluidic Device for Rat Bone Marrow Stromal Cell Differentiation Enhancement

Microfluidic devices provide low sample consumption, high throughput, high integration, and good environment controllability advantages. An alternative to conventional bioreactors, microfluidic devices are a simple and effective platform for stem cell investigations. In this study, we describe the design of a microfluidic device as a chemical and mechanical shear stress bioreactor to stimulate rat bone marrow stromal cells (rBMSCs) into neuronal cells. 1-methyl-3-isobutylxanthine (IBMX) was used as a chemical reagent to induce rBMSCs differentiation into neurons. Furthermore, the shear stress applied to rBMSCs was generated by laminar microflow in the microchannel. Four parallel microfluidic chambers were designed to provide a multiplex culture platform, and both the microfluidic chamber-to-chamber, as well as microfluidic device-to-device, culture stability were evaluated. Our research shows that rBMSCs were uniformly cultured in the microfluidic device and differentiated into neuronal cells with IBMX induction. A three-fold increase in the neuronal cell differentiation ratio was noted when rBMSCs were subjected to both IBMX and fluid flow shear stress stimulation. Here, we propose a microfluidic device which is capable of providing chemical and physical stimulation, and could accelerate neuronal cell differentiation from bone marrow stromal cells.
A Novel Angle Algorithm of Polarization Sensor for Navigation

Navigation technology is an essential ability for the survival and development of animal and human. Desert ants Cataglyphis are able to explore their desert habitat for hundreds of meters while foraging and return back to their nest precisely and in a straight line. For deriving direction information, these animals use the pattern of polarized light in the sky, which arises due to scattering of sunlight in the atmosphere. The polarization sensitivity mechanisms of the ants' compound eye are analyzed. A novel bionic navigation sensor is constructed. The work principle of the sensor is discussed. A novel algorithm of the direction angle calculation is presented. The merits of the new algorithm are discussed in detail. The angle output experiment of the navigation sensor is performed under different light conditions. The normalization output curve is obtained, and the angle output error is given with our algorithm. The experiment result indicates that the new angle algorithm is feasible and effective.
GPE: a new representation for VLSI floorplan problem

In this paper, we propose a new representation of VLSI floorplan and building block problem. The representation is the generalization of Polish expression. By proposing a new relational operator, the representation can efficiently reuse some area that cannot be utilized if only having vertical and horizontal operators defined in Polish expression, and is able to present non-slicing structural floorplan. The experimental results show that the representation achieves promising area utilization in commonly used MCNC benchmark circuits.
Arbitrage with fractional Brownian motion

In recent years fractional Brownian motion has been suggested to replace the classical Brownian motion as driving process in the modelling of many real world phenomena, including stock price modelling. In several papers seemingly contradictory results on the existence or absence of a riskless gain (arbitrage) in such stock models have been stated. This survey tries to clarify this issue by pointing to the importance of the chosen class of admissible trading strategies.
What About the Dads?: Child Welfare Agencies' Efforts to Identify, Locate and Involve Nonresident Fathers

Most foster children are not living with their fathers at the time they are removed from their homes. While in foster care these children may experience even less contact with their nonresident fathers. This study examined child welfare practices with respect to identifying, locating, and involving fathers of children in foster care including whether child support resources were used. Local agency caseworkers were interviewed by phone about nearly 2,000 foster children in four study states. The study found that nonresident fathers are not often involved in case planning and nearly half were never contacted by the child welfare agency.