Miracle-dz/UniFAR-FTUs huggingface dataset:
SAP155 Binds to ceramide‐responsive RNA cis‐element 1 and regulates the alternative 5′ splice site selection of Bcl‐x pre‐mRNA

Two splice variants are derived from the BCL‐x gene, proapoptotic Bcl‐x(s) and antiapoptotic Bcl‐x(L), via alternative 5′ splice site selection. In previous studies, our laboratory identified an RNA cis‐element within exon 2 of Bcl‐x pre‐mRNA that is a ceramide responsive termed CRCE 1. In this study, mass spectrometric analysis identified the splicing factor SAP155, as an RNA trans‐acting factor binding to the purine‐rich CRCE 1. The interaction of SAP155 with CRCE 1 was confirmed by the addition of an anti‐SAP155 antibody (Ab) to EMSA decreasing the mobility of a protein:CRCE 1 complex (SuperShift). Furthermore, the down‐regulation of SAP155 in A549 cells by RNA interference (RNAi) technology resulted in the loss of a 155 kDa protein complexed with CRCE 1. Moreover, this down‐regulation of SAP155 induced an increase in the Bcl‐x(s) with a concomitant decrease in the Bcl‐x(L) splice variants and immunoreactive protein levels, thereby decreasing the Bcl‐x(L)/Bcl‐x(s) ratio. Specific down‐regulation of SAP155 also inhibited the ability of exogenous ceramide treatment to further induce the activation of the Bcl‐x(s) 5′ splice site. Additionally, the specific down‐regulation of SAP155 sensitized cells to undergo apoptosis in response to daunorubicin in a manner similar to ceramide. Therefore, we have identified SAP155 as an RNA trans‐acting factor that binds to CRCE 1, functions to regulate the alternative 5′ splice site selection of Bcl‐x pre‐mRNA, and is required for ceramide to induce the activation of the Bcl‐x(s) 5′ splice site. Furthermore, we have demonstrated that activation of the Bcl‐x(s) 5′ splice site can increase the effectiveness of chemotherapeutic drug treatment, thus establishing a role for the alternative splicing mechanism of Bcl‐x in chemotherapeutic sensitivity. —Massiello, A. , Roesser, J. R. , Chalfant, C. E. SAP155 binds to ceramide‐responsive RNA cis‐element 1 and regulates the alternative 5′ splice site selection of Bcl‐x pre‐mRNA. FASEB J. 20, E921‐E929 (2006)
Bis{tris[3-(2-pyridyl)-1H-pyrazole]zinc(II)} dodecamolybdosilicate hexahydrate

The asymmetric unit of the title compound, [Zn(C8H7N3)3]2[SiMo12O40]·6H2O, consists of a complex [Zn(C8H7N3)3]2+ cation, half of a Keggin-type [SiMo12O40]4− heteropolyanion and three uncoordinated water molecules. The Zn2+ cation is surrounded in a distorted octahedral coordination by six N atoms from three chelating 3-(2-pyridyl)pyrazole ligands. In the heteropolyanion, two O atoms of the central SiO4 group ( symmetry) are equally disordered about an inversion centre. N—H⋯O hydrogen bonding between the cations, anions and the uncoordinated water molecules leads to a consolidation of the structure.
Computer Vision and Image Understanding Histogram of 3d Facets: a Depth Descriptor for Human Action and Hand Gesture Recognition

The recent successful commercialization of depth sensors has made it possible to effectively capture depth images in real time, and thus creates a new modality for many computer vision tasks including hand gesture recognition and activity analysis. Most existing depth descriptors simply encode depth information as intensities while ignoring the richer 3D shape information. In this paper, we propose a novel and effective de-scriptor, the Histogram of 3D Facets (H3DF), to explicitly encode the 3D shape information from depth maps. A 3D Facet associated with a 3D cloud point characterizes the 3D local support surface. By robust coding and circular pooling 3D Facets from a depth map, the proposed H3DF descriptor can effectively represent both 3D shapes and structures of various depth maps. To address the recognition problems of dynamic actions and gestures, we further extend the proposed H3DF by combining it with an N-gram model and dynamic programming. The proposed descriptor is extensively evaluated on two public 3D static hand gesture datasets, one dynamic hand gesture dataset, and one popular 3D action recognition dataset. The recognition results outperform or are comparable with state-of-the-art performances.
Initial degradation of deoxyribonucleic acid after injection in mammals.

The fate of DNA[2][1] in the blood of rabbits and mice, after i.v. and i.p. injection, has been followed by viscosimetry and chemical determination of acid-precipitable DNA. It has been shown that the initial degradation of DNA is due to neutral DNase activity, which sets up a very effective biochemical barrier against exogenous DNA. The change in molecular weight of DNA administered in vivo has been calculated and correlated with the time after which physical properties of DNA are not consistent with biologic activity. This time can be expected to vary from a few sec to about 10 min in animal species with low plasma DNase level, such as man. It can be increased by using DNase-inhibitors like methyl green. Although it is unlikely that a large amount of highly polymerized DNA could reach cells without previous depolymerization after i.v. administration, our results are compatible with the expression of any infecting or transforming properties of this material. [1]: #fn-2
Pertuzumab monotherapy after trastuzumab-based treatment and subsequent reintroduction of trastuzumab: activity and tolerability in patients with advanced human epidermal growth factor receptor 2-positive breast cancer.

PURPOSE The combination of pertuzumab and trastuzumab resulted in a clinical benefit rate (CBR) of 50% in patients with human epidermal growth factor receptor 2 (HER2) -positive breast cancer whose disease progressed during prior trastuzumab-based therapy. To define whether this previously observed encouraging activity was a result of the combination of pertuzumab and trastuzumab or of pertuzumab alone, we recruited a third cohort of patients who received pertuzumab without trastuzumab. We then investigated the impact of reintroducing trastuzumab to patients whose disease progressed on pertuzumab monotherapy. PATIENTS AND METHODS Twenty-nine patients with HER2-positive breast cancer whose disease progressed during prior trastuzumab-based therapy received pertuzumab (840 mg loading dose, then 420 mg every 3 weeks) until progressive disease or unacceptable toxicity. Seventeen patients with disease progression continued to receive pertuzumab (at the same dose), with the addition of trastuzumab (4 mg/kg loading dose and then 2 mg/kg weekly or 8 mg/kg loading dose and then 6 mg/kg every 3 weeks). RESULTS All 29 patients enrolled for pertuzumab monotherapy experienced disease progression. The objective response rate (ORR) and CBR were 3.4% and 10.3%, respectively, during pertuzumab monotherapy. With the addition of trastuzumab, the ORR and CBR were 17.6% and 41.2%, respectively. Progression-free survival was longer with combination therapy than pertuzumab monotherapy (17.4 v 7.1 weeks, respectively). Treatment was well tolerated with minimal cardiac dysfunction. CONCLUSION Although pertuzumab has some activity in patients with HER2-positive breast cancer that progressed during therapy with trastuzumab, the combination of pertuzumab and trastuzumab seems to be more active than monotherapy.
Cost-effectiveness Analysis of Follow-up Strategies for Thunderclap Headache Patients With Negative Noncontrast CT.

OBJECTIVES Accurate diagnosis of acute subarachnoid hemorrhage (SAH) is critical in thunderclap headache patients due to high morbidity and mortality associated with missed aneurysmal bleeds. The objective of this study was to determine the utility of computed tomography angiography (CTA) in managing patients with acute, severe headaches and negative noncontrast CT and assess the cost-effectiveness of three different screening strategies-no follow up, CTA, and lumbar puncture (LP). METHODS A modeling-based economic evaluation was performed with a time horizon of 1 year for thunderclap headache patients in the emergency department with negative noncontrast CT for SAH. Sensitivity analyses were performed to determine the effect of sensitivity of CT and the prevalence of SAH on cost-effectiveness. RESULTS Lumbar puncture follow-up has the lowest cost and the highest utility in the mathematical model. The Monte Carlo simulation shows noncontrast CT with LP follow-up to be the most cost-effective strategy in 85.3% of all cases even at a $1 million/quality-adjusted life-years willingness-to-pay. Sensitivity analyses demonstrate that LP follow-up should be performed, except for when CT sensitivity exceeds 99.2% and the SAH prevalence is below 3.2%, where no follow-up may be considered. CONCLUSIONS Although CTA is frequently used for evaluation of thunderclap headache patients, its utility is not clearly defined. LP follow-up is shown to be the most cost-effective strategy for evaluation of thunderclap headache patients in most clinical settings.
Alcoholic subtypes: are two sufficient?

AIMS Guided by the literature on alcohol subtypes, cluster analytical solutions ranging from two to five were evaluated for a large (n = 802), ethnically diverse sample of alcoholic in-patients. DESIGN Personal interview data were collected from in-patients regarding their substance abuse and psychiatric disorder status, risk factors for psychopathology and health outcomes. SETTING The data were collected at five alcohol in-patient treatment settings in New York; three settings were in New York City, one in Rochester and one in Buffalo. PARTICIPANTS The sample included 802 participants (481 males and 321 females) with racial/ethnic group diversity (418 blacks, 180 whites, 187 Hispanics, 17 of other ethnic origin). MEASUREMENTS Subjects were assessed with standardized measures of substance abuse and psychiatric disorders, family history of alcoholism, psychopathy, temperament, alcohol expectancies and clinical health variables. FINDINGS Based on internal and external criteria and compatibility with the existing literature, four subtypes were identified: mild course, polydrug, negative affect and chronic/antisocial. On external criteria, the polydrug subtype had the highest rate of family criminality, high-risk sexual behavior and intravenous drug use; the negative affect subtype had the highest rate of childhood sexual abuse, attempted suicide and childhood homelessness; the chronic/antisocial personality (ASP) subtype had the most severe pattern of drinking and antisocial behavior. CONCLUSIONS Findings are discussed with regard to the etiological and clinical utility of the four-subtype formulation, and consistency with recent studies in molecular genetics and pharmacology.
Organized hematoma of the maxillary sinus: CT findings.

OBJECTIVE Organized hematoma of the maxillary sinus is rare. It occurs by organization of the hematoma in an unaerated maxillary sinus. The purpose of this study was to evaluate the CT features of this lesion. CONCLUSIONS Organized hematoma should be included in the differential diagnosis when patients have recurrent epistaxis and an expansile mass in the maxillary sinus.
A proposal for computation of semantic similarities of Chinese automation discipline words

In dealing with the Semantic similarity of Chinese words, using Hownet would be a preferable popular method. But HowNet has its own shortcomings. First, most of the concepts in HowNet concern in explaining common terms, lacking of specialized vocabulary. Second, most of the concepts in HowNet are independent concepts and lack of compound concepts. In response to the above problems, we combine HowNet with Conceptual Knowledge Tree Knowledge Representation System. Based on this, we have analyzed the structures of concepts, distributed different weights to relevant positions, determined their meanings and calculated Chinese semantic similarity of automation discipline. The results are satisfactory basically.
Ontology learning and population from text - algorithms, evaluation and applications

In the last decade, ontologies have received much attention within computer science and related disciplines, most often as the semantic web. Ontology Learning and Population from Text: Algorithms, Evaluation and Applications discusses ontologies for the semantic web, as well as knowledge management, information retrieval, text clustering and classification, as well as natural language processing. Ontology Learning and Population from Text: Algorithms, Evaluation and Applications is structured for research scientists and practitioners in industry. This book is also suitable for graduate-level students in computer science.
Robust geocast routing protocols for safety and comfort applications in VANETs

Routing in cooperative vehicular networks is a challenging task because of high mobility of vehicles and difficulty of localization. In this paper, we study the geocast routing problem in Vehicular Ad-hoc NETworks VANETs, which aims at delivering data to a specific group of mobile vehicles identified by their geographical location. Although many geocast routing protocols have been proposed, only partial inherent constraints of VANETs such as mobility, internal network fragmentation problem, external network fragmentation problem, and overload are taken into account. Therefore, we propose two novel and robust geocast routing protocols: the first one, called Robust Geocast Routing Protocol for Safety Applications RGRP-SA, is dedicated to road safety applications, while the second, namely, Robust Geocast Routing Protocol for Comfort Applications RGRP-CA, is designed for comfort applications. Simulations conducted in NS-2 demonstrate that our safety-oriented RGRP-SA protocol outperforms Inter-Vehicle Geocast protocol and Mobicast Routing Protocol in VANETs by sending up to 25% more packets, cutting the end-to-end delay in half, and solving the internal network fragmentation problem. Besides, it is also shown that our comfort-oriented RGRP-CA protocol serves well comfort applications with only light overhead by solving internal and external network fragmentation problems and providing more reliable data delivery with a 25% higher packet delivery ratio and higher network throughput utilization in comparison with Mobicast with Carry-and-Forward protocol. Copyright © 2015 John Wiley & Sons, Ltd.
Mitigating Snell's-Law reflection and transmission with metasurfaces of ordinary dielectrics

Obtaining anomalous reflection and transmission (namely propagation along directions different from those predicted by Snell's law) is investigated for plane waves impinging on a planar periodic metasurface composed of two dielectric layers. The associated boundary-value problem is treated by a rigorous entire-domain integral equation methodology. Optimizations with respect to the configuration's parameters are performed which reveal that it is possible to obtain significantly enhanced anomalous transmission and reflection depending on the color of the incident light. The optimal parameter values correspond to metasurfaces which can be easily realizable by low-loss dielectric materials.
Current control of IPMSM servo system in field-weakening region via DOB-based model predictive control

Current control of IPMSM servo system is investigated in this paper. Under the field-weakening control framework, nonlinear couplings of the voltage equations are very strong. The current control performance may not be well until such nonlinear couplings are well dealed. In this case, a DOB-based model predictive control is proposed to improve the current control performance under field-weakening control framework. The proposed control method consists of a forecasted model based on disturbance observer, feedback correction for model mismatch rejection, and receding optimization for an optimized control input. Numerical simulations demonstrate that proposed method achieves nonlinear coupling attenuation ability and well promising field-weakening control performance.
Novel Right-Handed Metamaterial Based on the Concept of “Autonomous Control System of Living Cells” and Its Absorber Applications

A novel right-handed metamaterial similar to an “autonomous control system of a living cell” and capable of responding to electromagnetic waves is proposed with its fundamental configurations. Using microwave absorbers as an example, the possibility of equivalently controlling their material constants are investigated both theoretically and empirically. These absorbers are composed of unit cells based on the concept of an autonomous controllable metamaterial (ACMM). The theoretical approaches used to obtain ACMM-based absorber design data are revealed. In order to improve the absorber matching characteristics, the principle of obtaining the broadband matching characteristics with an absorption band of 1.3 GHz at 4.25 GHz is clarified. In addition, the methods for improving the matching degradation of the oblique incidence of a TM wave are investigated. For the TM wave oblique incidence, a new ACMM-based absorber configuration comprising unit cells with inductive fins is proposed. At an incident angle of 45°, a matching characteristic of -30 dB is achieved in the case of the TM wave. These ACMMs are controlled by using only two bias feeder wirings for PIN diodes.
Risk assessment in patients with ST elevation myocardial infarction treated with primary percutaneous coronary angioplasty. Does reduced duration of hospital stay affect the prognosis?

Marcin Dębiński, MD, PhD, specialist in internal medicine and cardiology. Interventional cardiologist in the American Heart of Poland 3rd Department of Invasive Cardiology, Angiology, and Electrophysiology in Dabrowa Gornicza. Co-inventor of the animal myocardial infarction model, on the basis of which in 2006 he defended his doctoral thesis on reperfusion injury. Main areas of interest: invasive treatment of myocardial infarction, multivessel and left main coronary artery disease, and aortic stenosis. Since 2012 a member of the Transcatheter Aortic Valve Implantation (TAVI) programme. Author and co-author of publications in peer-reviewed journals.
The World of the Golden Ass

Those who study and teach the history of the Ancient World suffer from a great disadvantage, which we find difficult to admit even to ourselves: in a perfectly literal sense we do not know what we are talking about. Of course we can dispose of a vast range of accumulated knowledge about what we are talking about. We can compile lists of officeholders in the Roman Empire, without our evidence revealing how government worked or even whether it made any impact at all on the ordinary person; we can discuss the statuses of cities and look at the archaeological remains of some of them (or rather some parts of some of them) without having any notion of their social and economic functions, or of whether it made any real difference whether an inhabitant of the Roman provinces lived in a small city or a large village.
Calcium Signaling and Meiotic Exit at Fertilization in Xenopus Egg

Calcium is a universal messenger that mediates egg activation at fertilization in all sexually reproducing species studied. However, signaling pathways leading to calcium generation and the mechanisms of calcium-induced exit from meiotic arrest vary substantially among species. Here, we review the pathways of calcium signaling and the mechanisms of meiotic exit at fertilization in the eggs of the established developmental model, African clawed frog, Xenopus laevis. We also discuss calcium involvement in the early fertilization-induced events in Xenopus egg, such as membrane depolarization, the increase in intracellular pH, cortical granule exocytosis, cortical contraction, contraction wave, cortical rotation, reformation of the nuclear envelope, sperm chromatin decondensation and sister chromatid segregation.
Decellularized Scaffolds: Concepts, Methodologies, and Applications in Cardiac Tissue Engineering and Whole-Organ Regeneration

Tissue engineering research, which aims to develop tissue/organ substitutes for treating pathological disorders and organ failures, has made many breakthroughs during the past three decades. The fi eld still faces challenges such as identifying and optimizing scaffolds that must be biodegradable, non-immunogenic, and able to provide structural, mechanical, biological supports/cues for cell adhesion, proliferation, and differentiation. Recent accomplishments in tissue decellularization provide acellular tissue-derived scaffolds that retain the nature-designed structure from the whole organ level, to the microstructural scale, down to the nanoscale. The preservation of structurally organized entities such as collagen, elastin, glycosaminoglycans, and fi bronectin enables a natural template that accommodates many tissue
New Mos 1 mariner transposons suitable for the recovery of gene fusions in vivo and in vitro

The Drosophila Mos1 element can be mobilized in species ranging from prokaryotes to protozoans and vertebrates, and the purified transposase can be used for in vitro transposition assays. In this report we developed a ‘mini-Mos1’ element and describe a number of useful derivatives suitable for transposon mutagenesis in vivo or in vitro. Several of these allow the creation and/or selection of tripartite protein fusions to a green fluorescent protein–phleomycin resistance (GFP-PHLEO) reporter/selectable marker. Such X-GFP-PHLEO-X fusions have the advantage of retaining 5 0 and 3 0 regulatory information and Nand C-terminal protein targeting domains. A Mos1 derivative suitable for use in transposon-insertion mediated linker insertion (TIMLI) mutagenesis is described, and transposons bearing selectable markers suitable for use in the protozoan parasite Leishmania were made and tested. A novel ‘negative selection’ approach was developed which permits in vitro assays of transposons lacking bacterial selectable markers. Application of this assay to several Mos1 elements developed for use in insects suggests that the large mariner pM[cn] element used previously in vivo is poorly active in vitro, while the Mos1-Act-EGFP transposon is highly active. q 2001 Elsevier Science B.V. All rights reserved.
Object Grasping with the NAO

With autonomous robots becomingmore andmore common, the interest in applications of mobile robotics increases. Many applications of robotics include the grasping and manipulation of objects. As many robotic manipulators have several degrees of freedom, controlling these manipulators is not a trivial task. The actuator needs to be guided along a proper trajectory towards the object to grasp, avoiding collisions with other objects and the surface supporting the object. In this project, the problem of learning a proper trajectory towards an object to grasp, located in front of a humanoid robot, the Aldebaran NAO, is solved by using machine learning. Three algorithms were evaluated. Learning from demonstration using a neural network trained on a training set of recorded demonstrations was not capable of learning this task. Using Nearest Neighbor on the same training set yielded much better results in simulation but had more problems picking up objects on the real robot. A form of Reinforcement Learning (RL) tailored to continuous state and action spaces, the Continuous Actor Critic Learning Automaton (CACLA), proved to be an effective way to learn to solve the problem by exploring the action space to obtain a good trajectory in a reasonable amount of time. This algorithm also proved to be robust against the additional complexity of operating on the real robot after being trained in simulation, bridging the reality gap.