Miracle-dz/UniFAR-FTUs huggingface dataset:
Z-DNA's Conformer Substates Revealed by FT-IR Difference Spectroscopy of Nonoriented Left-Handed Double Helical Poly(dG-dC)

Abstract Nonoriented hydrated films of double helical poly(dG-dC) in the Z-form were studied by Fourier transform infrared (FT-IR) spectroscopy either as equilibrated slow-cooled samples between 290 and 220 K or, after quenching into the glassy state, as nonequilibrated film isothermally at 200, 220, and 240 K. IR spectral changes on isothermal relaxation at 200 and 220 K toward equilibrium, caused by interconversion of two conformer substates (CS) called Z1 and Z2, are revealed by IR difference spectra. Pronounced spectral changes on Z1-to-Z2 interconversion occur between ≈750–1250 cm−1 and these are attributed to structural changes of the phosphodiester-sugar backbone caused by changes of torsion angles, and to decreasing hydrogen-bonding of the ionic phosphate group with water molecules. These spectral changes on Z1-to-Z2 transition can be related to structural differences between ZI and ZII CS observed in single crystals. ZI/ZII CS occurs only at (dGpdC) base steps, and similar behavior is assumed for Z1/Z2. The Z1/Z2 population ratio was determined via curve resolution of marker bands for Z1 and Z2 centered at 785 and 779 cm−1. This ratio is 0.64 at 290 K, corresponding to 39% of the phosphates of the (dGpdC) base steps in Z1 and 61% in Z2, and it increases to 1.24 on cooling to 220 K. For the Z2⇔Z1 equilibrium, an enthalpy change of −4.9 ± 0.2 kJ mol(dGpdC)−1 is obtained from the temperature dependence of the equilibrium constant. Z1 interconverts into Z2 at isothermal relaxation at 200 and 220 K, whereas on slow cooling from ambient temperature, Z2 interconverts into Z1. This unexpected reversal of CS interconversion is attributed to slow restructuring of hydration shells of the CS on quenching, in the same manner reported by Pichler et al. for the BI and BII CS of B-DNA (J. Phys. Chem. B 106, 3263–3274 (2002)). IR difference curves demonstrate two time scales on isothermal relaxation of Z1→Z2 interconversion, a fast one for structural relaxation of the sugar-phosphate backbone, and a slow one for relaxation of the hydration shells. This slowing down of restructuring of CS hydration shells at ≈220–240 K could be the cause for the suppression of biological functions at low temperatures.
The Global Acetylome of the Human Pathogen Vibrio cholerae V52 Reveals Lysine Acetylation of Major Transcriptional Regulators

Protein lysine acetylation is recognized as an important reversible post translational modification in all domains of life. While its primary roles appear to reside in metabolic processes, lysine acetylation has also been implicated in regulating pathogenesis in bacteria. Several global lysine acetylome analyses have been carried out in various bacteria, but thus far there have been no reports of lysine acetylation taking place in the important human pathogen Vibrio cholerae. In this study, we analyzed the lysine acetylproteome of the human pathogen V. cholerae V52. By applying a combination of immuno-enrichment of acetylated peptides and high resolution mass spectrometry, we identified 3,402 acetylation sites on 1,240 proteins. Of the acetylated proteins, more than half were acetylated on two or more sites. As reported for other bacteria, we observed that many of the acetylated proteins were involved in metabolic and cellular processes and there was an over-representation of acetylated proteins involved in protein synthesis. Of interest, we demonstrated that many global transcription factors such as CRP, H-NS, IHF, Lrp and RpoN as well as transcription factors AphB, TcpP, and PhoB involved in direct regulation of virulence in V. cholerae were acetylated. In conclusion, this is the first global protein lysine acetylome analysis of V. cholerae and should constitute a valuable resource for in-depth studies of the impact of lysine acetylation in pathogenesis and other cellular processes.
Multi-fold Gabor filter convolution descriptor for face recognition

The standard multi-scale, multi-orientation Gabor filter ensemble (SGFE) in the face recognition task reposits 40 filters localized in 8 orientations and 5 scales, with a real and an imaginary constituent. This paper devises a simple means of filter diversification, dubbed as multi-fold Gabor filter convolution (-FGFC), where a set of pre-selected filters, e.g., single-scale Gabor filters across varying orientations, are self-cross convolved by folds to instantiate the offspring filters. To facilitate filter selection for-FGFC, this paper summarizes SGFE into the condensed Gabor filter ensemble (CGFE) of only 8 filters. In addition, an average histogram pooling operator is proposed to downsample and regulate the demodulated Gabor phase features prior to the final compression stage. The performance of a specific M-FGFC instance, i.e., the 2-FGFC descriptor, is investigated on FERET I (frontal), FERET II (nonfrontal) and AR datasets. The experimental results on FERET I substantiates that the 2-FGFC descriptor outperforms the leading state of the art face descriptors.
EVALUATION OF ANTIBACTERIAL, ANTITUMOR, ANTIOXIDANT ACTIVITIES AND PHENOLIC CONSTITUENTS OF FIELD-GROWN AND IN VITRO-GROWN LYSIMACHIA VULGARIS L

Background: Lysimachia vulgaris L. (Yellow loosestrife) is a medicinal plant in the family Myrsinaceae. It has been used in the treatment of fever, ulcer, diarrhea and wounds in folk medicine. It has also analgesic, expectorant, astringent and anti-inflammatory activities. Two different sources of the plant (field-grown and in vitro-grown) were used to evaluate the biological activities (antibacterial, antitumor and antioxidant) of L. vulgaris. In vitro-grown plant materials were collected from L. vulgaris plants that were previously regenerated in our laboratory. Materials and Methods: Plant materials were extracted with water, ethanol and acetone. For antibacterial test, disc diffusion method and 10 different pathogenic bacteria were used. Antioxidant activity was indicated by using DPPH method. The total phenol amount by using Folin-Ciocaltaeu method and the total flavonoid amount by using aluminum chloride (AlCl3) colorimetric method were determined. Results: Generally, yellow loosestrife extracts demonstrated antibacterial activity against Gram-positive bacteria (Staphylococcus aureus, S. epidermidis and Streptococcus pyogenes). Strong antitumor activity of yellow loosestrife was observed via potato disc diffusion bioassay. Nine different phenolics were also determined and compared by using High-Performance Liquid Chromatography (HPLC). Conclusion: Future investigations should be focused on fractionation of the extracts to identify active components for biological activity.
Symbol-Based Algorithms for Decoding Binary LDPC Codes with Higher-Order Modulations

Low-density parity-check (LDPC) codes are widely used in communication systems. When user messages are encoded by binary LDPC codes and transmitted with higher-order modulations, bits composing the same modulated symbol are generally correlated. Traditional soft-decision decoding algorithms for binary LDPC codes like sum-product algorithm (SPA) and min-sum algorithm (MSA) update messages only based on log-likelihood ratio (LLR) of bits, and correlations introduced by modulation are not utilized. In this paper, symbol-based SPA and MSA (S-SPA and S-MSA) which incorporate symbol-level information in the decoding procedures are proposed to provide an alternative low-cost way to better utilize available information. By narrowing the search space of symbols based on bit-level information, the complexities of the proposed algorithms are independent of modulation order. S-SPA and S-MSA achieve better error-correction performances than SPA and MSA with little additional complexities and S-MSA is friendly to hardware design of LDPC decoder.
PESQUISAS INTERNACIONAIS RECENTES EM ESTRUTURA DE CAPITAL RECENT INTERNATIONAL RESEARCH IN CAPITAL STRUCTURE

Capital Structure is still one of controversial themes in corporate finance, which allows many research activities both on the theories that have been widely discussed but also on the several interrelationships with other corporate aspects. The aim of this article was to review the most recent academic publication of researches involving capital structure of the firms. This study focuses on the last 3 years, from 2011 to 2013, and on the international scientific journals in Finance that present the highest impact factors, namely: The Review of Financial Studies, The Journal of Finance, Journal of Financial Economics e Journal of Banking & Finance. Despite of having been widely discussed, the differences between the main theories on capital structure, the trade-off theory and the pecking order theory, are still object of researches – however, maybe for being the most recent, the theory of equity market timing has been the most discussed theme in the last 3 years. Besides these topics, the researchers have dealt with many aspects of the corporate variables that are direct or indirectly associated with the capital structure decisions. This article presents a brief summary of several publications allowing a general overview on the recent researches. Pesquisas Internacionais Recentes em Estrutura de Capital Edelcio Koitiro Nisiyama, Wilson Toshiro Nakamura Revista de Administração de Roraima-RARR, Boa Vista, ISSNe 2237-8057 • Vol. 5 n. 1, jan -jun. 2015. p. 105-123 106
A mathematical model for supplier selection in quantity discount environments

Supplier selection has received extensive attention in supply chain management (SCM). In this field, owing to the difficulty of treating non-linear functions, many SCM models assume that the prices of materials, production, transportation and inventory are constant. This is far beyond the real situations and, however, is not practical. In fact, vendors usually offer quantity discounts to encourage the buyers to order more, and the producer intends to discount the unit production cost if the amount of production is large. For dealing with this difficulty, this paper examines the application of data envelopment analysis, which has demonstrated potential as a management tool to overcome the shortcomings of other techniques and to help purchasing executives improve the efficiency of their operations.
DGGE and real-time PCR analysis of lactic acid bacteria in bacterial communities of the phyllosphere of lettuce.

Food associated indigenous microbial communities exert antagonistic effects on pathogens and may routinely deliver health relevant microorganisms to the GI tract. By using molecular, culture independent methods including PCR-DGGE of 16S rDNA-coding regions and real-time PCR (RT-PCR) as well as BIOLOG metabolic fingerprinting, microbial communities on lettuce were analyzed in samples from fields, from supermarkets and soil. Amplified 16S rRNA gene sequences (57.7%) could be assigned to species previously reported as typical for the phyllosphere including Pantoea agglomerans, Pseudomonas flavescens, Moraxella spp. , and Mycobacterium spp. 71.8% of the sequences obtained represented so far undescribed taxa. Principal component analysis of BIOLOG metabolic profiles indicated a seasonal variation in the lettuce phyllosphere microbial community structure. Various lactic acid bacteria were detected including several Lactobacillus and Leuconostoc species in particular on lettuce from organic farming. By RT-PCR lactobacilli were found with a range of abundances from 1x10(4 )to 1x10(5 )copies/g lettuce. Considering the importance of salad in many diets lettuce may contribute to a constant supply with LAB.
CGRP Inhibits Neurons of the Bed Nucleus of the Stria Terminalis : Implications for the Regulation of Fear and Anxiety

The bed nucleus of the stria terminalis (BNST) is thought to generate anxiety-like states via its projections to autonomic and neuroendocrine regulatory structures of the brain. However, because most BNST cells are GABAergic, they are expected to inhibit target neurons. In contrast with this, infusion of calcitonin gene-related peptide (CGRP) into BNST was reported to potentiate anxiety while activating BNST targets. The present study aimed to shed light on this paradox. The CGRP innervation of BNST originates in the pontine parabrachial nucleus and targets its anterolateral sector (BNST-AL). Thus, we investigated the effects of CGRP on BNST-AL neurons using patch recordings in vitro in male rats. CGRP did not alter the passive properties of BNST-AL cells but increased the amplitude of IPSPs evoked by stimulation of the stria terminalis (ST). However, IPSP paired-pulse ratios were unchanged by CGRP, and there was no correlation between IPSP potentiation and variance, suggesting that CGRP acts postsynaptically. Consistent with this, CGRP hyperpolarized the GABA-A reversal of BNST-AL cells. These results indicate that CGRP increases ST-evoked GABA-A IPSPs and hyperpolarizes their reversal potential through a postsynaptic change in Cl homeostasis. Overall, our findings suggest that CGRP potentiates anxiety-like behaviors and increases neural activity in BNST targets, by inhibiting BNST-AL cells, supporting the conclusion that BNST-AL exerts anxiolytic effects.
Abundance and characteristics of Pisolithus ectomycorrhizas in New Zealand geothermal areas

Pisolithus is restricted in New Zealand to geothermal areas where it associates with Kunzea ericoides var. microflora (prostrate kanuka) and occasionally Leptospermum scoparium. Here we describe for the first time the ectomycorrhizal morphotypes of three New Zealand Pisolithus species and report the frequency and abundance of these morphotypes against other mycorrhizal fungi associated with these hosts in New Zealand geothermal areas. The three Pisolithus species form typical ectomycorrhizal associations with Kunzea ericoides var. microflora, and one also was observed forming typical ectomycorrhizal associations with Leptospermum scoparium. Although the morphotypes from the three Pisolithus species share many morphological and anatomical characteristics, they vary with regard to the abundance of rhizomorphs. The common occurrence of Pisolithus fruiting bodies at the geothermal sites was matched by frequent and abundant Pisolithus ectomycorrhizas. Pisolithus ectomycorrhizas were frequent (100% of soil cores) and abundant (between 55 and 88% of ectomycorrhizal tips) associates of prostrate kanuka in hot (50 C at 8 cm depth), highly acidic and N depleted soils. The levels of arbuscular mycorrhizal colonization of prostrate kanuka were lower than on K. ericoides and L. scoparium on cooler soils. The stressful conditions where prostrate kanuka dominates probably favor Pisolithus over the mycorrhizal fungi occurring in cooler geothermal areas. Questions about how several genetically similar Pisolithus species co-occur on prostrate kanuka in geothermal areas without mutual competitive exclusion are discussed.
Improved Light Output Power of InGaN/GaN LED by Using Ultrasonic Spray Pyrolysis Deposited MgO

This paper presents an MgO thin films deposited by an ultrasonic spray pyrolysis deposition method to serve as passivation and antireflection layers of the InGaN/GaN light-emitting diodes (LEDs). The electrical, electroluminescence (EL), and efficiency characteristics of the LEDs with 60-, 70-, and 80-nm MgO films are investigated. It is found that the LEDs with the MgO films have lower reverse currents and higher shunt resistance than that without an MgO film. However, there are no significant differences of the forward voltage at 350 mA and the series resistance between the LEDs with and without the MgO films. The significant differences between the LEDs with and without the MgO films are the light output power (LOP) and efficiency. The LED with 70-nm MgO film has the highest LOP of 556 mW (at 700 mA) and external quantum efficiency of 40.8% (at 30 mA). Further, the LED chips with the MgO films show better LOP and wall plug efficiency than those with the SiO2 and SiNx films. It is also found that the LED chips with MgO film as a passivation layer shows stable electrical and EL characteristics in the aging time test.
Shear-induced distortion of intramolecular and intermolecular correlations in liquids: Time-dependent density-functional theory.

Shear viscosity of molecular liquids is formulated and calculated with use of a time-dependent density-functional theory. Allowing for nonrigidity of each molecule, we obtain shear-induced distortion of both intramolecular and intermolecular correlations self-consistently, which makes it possible to calculate the shear viscosity. As a by-product we derive and solve a hypernetted-chain closure for equilibrium correlations in deformable molecular liquids. \textcopyright{} 1996 The American Physical Society.
Localized Harris-FAST interest point detector

Simultaneous Localization and Mapping (SLAM) requires both rotation and scale invariant features. Few algorithms have been developed with rotation and scale invariant features with few limitations. Thus, an algorithm has been proposed to address rotation and scaling invariance. Proposed algorithm Harris-FAST interest point detector is a fusion of Harris and FAST interest point detectors. The detector is optimized by localizing the interest point detector to preferred area, from which the execution time of the algorithm reduced to the order of one fourth. The results of the proposed algorithm are compared with available feature detectors for its performance. Localized-Harris-FAST algorithm has been developed with an interest point detector for use in real time applications like SLAM on a mobile robot, which have limited computational resources.
Long-term investment sensitivity to cash flow and financial crisis

ABSTRACT This study examined investment–cash flow sensitivity in unconstrained and constrained firms from 1980 to 2010 in a sample of Italian manufacturing firms. Investment sensitivity to cash flow decreased over time, and financially constrained firms showed little difference in the decline compared with unconstrained firms. Last, investment sensitivity to cash flow increased during the financial crisis of 2008.
Contributions (in 2005) of marine and fresh water products (finfish and shellfish, seafood, wild and farmed) to the French dietary intakes of vitamins D and B12, selenium, iodine and docosahexaenoic acid: impact on public health

Primary objective To assess the contributions of seafood (finfish and shellfish, wild and farmed, freshwater and marine) to the French recommended daily intakes of dietary elements that are particularly abundant in these foods. Methods and procedures We first calculated the concentrations of each of these elements by critical analysis of a large body of published data. We then determined the consumption of seafood in France (in 2005) using a modified version of the method of measuring dietary intake defined by the FAO. Main outcomes and results The percentages of the French recommended daily intakes obtained from seafood species are: 44% for vitamin D, 65% for vitamin B12, 24% for selenium, 21% for iodine and 156% for an omega-3 fatty acid, docosahexaenoic acid (DHA) (159% referring to the French AFSSA seafood data). Conclusions French people must increase their seafood consumption to counteract disorders arising due to the demonstrated low concentrations of these nutrients in their usual diets; this could help overcome a potentially major public health problem.
The Effect of Guided Imagery Techniques to Decrease Students’ Anxiety in Group Counseling Activities

Relaxation techniques such as guided imagery has been used to treat a variety of student anxiety-related problems. The purpose of this study was to describe effect influence of guided imagery techniques to decrease the level of student’s anxiety in group counseling activities. This research was quasiexperiment with one group pretest-posttest design. The samples, taken by purposive sampling, were eight junior high school students who had high anxiety level when participating in group counseling. The results analyzed by z test indicated that there was declining level of the student’s anxiety in following group counseling activities, the value of z = -2.521 (p< 0.05). This finding explains that there was effect of the use of guided imagery techniques to the level of student anxiety in following group counseling activities. Therefore, guide imagery technique can be used effectively in group counseling for Junior High School Students. Keywords—student anxiety; group counseling; guided imagery
Advantages and potential challenges of data management in e-maintenance

Purpose The purpose of this paper is twofold: first, to understand data management challenges in e-maintenance systems from a holistically viewpoint through summarizing the earlier scattered research in the field, and second, to present a conceptual approach for addressing these challenges in practice. Design/methodology/approach The study is realized as a combination of a literature review and by the means of analyzing the practices on an industry leader in manufacturing and maintenance services. Findings This research provides a general understanding over data management challenges in e-maintenance and summarizes their associated proposed solutions. In addition, this paper lists and exemplifies different types and sources of data which can be collected in e-maintenance, across different organizational levels. Analyzing the data management practices of an e-maintenance industry leader provides a conceptual approach to address identified challenges in practice. Research limitations/implications Since this paper is based on studying the practices of a single company, it might be limited to generalize the results. Future research topics can focus on each of mentioned data management challenges and also validate the applicability of presented model in other companies and industries. Practical implications Understanding the e-maintenance-related challenges helps maintenance managers and other involved stakeholders in e-maintenance systems to better solve the challenges. Originality/value The so-far literature on e-maintenance has been studied with narrow focus to data and data management in e-maintenance appears as one of the less studied topics in the literature. This research paper contributes to e-maintenance by highlighting the deficiencies of the discussion surrounding the perspectives of data management in e-maintenance by studying all common data management challenges and listing different types of data which need to be acquired in e-maintenance systems.
A Supervisory Power Management System for a Hybrid Microgrid With HESS

This paper proposes a supervisory power management system (PMS) for a grid interactive microgrid with a hybrid energy storage system. The key feature of the proposed PMS is reduced number of sensors required to implement the PMS. The PMS considers renewable power variation, grid availability, electricity pricing, and changes in local loads. It can detect the operating mode of system without measuring load currents and powers. A single-phase voltage source converter (VSC) transfers real power between dc grid and utility grid besides offering ancillary services such as harmonic mitigation, reactive power support, and unity power factor at the point of common coupling (PCC). In the proposed system, a better dc-link voltage regulation is achieved and the usage of supercapacitors reduces the current stress on the battery. The PMS also addresses extreme operating conditions such as load shedding, off-maximum power point tracking operation of photovoltaic, elimination of critical oscillation of hybrid energy storage systems power, islanded operation, and resynchronization with grid. The performance of the proposed PMS is verified by digital simulation and experimental studies.