Miracle-dz/UniFAR-FTUs huggingface dataset:
Abolishing Cell Wall Glycosylphosphatidylinositol-Anchored Proteins in Candida albicans Enhances Recognition by Host Dectin-1

ABSTRACT Fungi can shield surface pathogen-associated molecular patterns (PAMPs) for evading host immune attack. The most common and opportunistic human pathogen, Candida albicans, can shield β-(1 3)-glucan on the cell wall, one of the major PAMPs, to avoid host phagocyte Dectin-1 recognition. The way to interfere in the shielding process for more effective antifungal defense is not well established. In this study, we found that deletion of the C. albicans GPI7 gene, which was responsible for adding ethanolaminephosphate to the second mannose in glycosylphosphatidylinositol (GPI) biosynthesis, could block the attachment of most GPI-anchored cell wall proteins (GPI-CWPs) to the cell wall and subsequently unmask the concealed β-(1,3)-glucan. Neutrophils could kill the uncloaked gpi7 mutant more efficiently with an augmented respiratory burst. The gpi7 mutant also stimulated Dectin-1-dependent immune responses of macrophages, including activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways and secretion of specific cytokines, such as tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and IL-12p40. Furthermore, the gpi7 null mutant could induce an enhanced inflammatory response through promoting significant recruitment of neutrophils and monocytes and could stimulate stronger Th1 and Th17 cell responses to fungal infections in vivo. These in vivo phenotypes also were Dectin-1 dependent. Thus, we assume that GPI-CWPs are involved in the immune mechanism of C. albicans escaping from host recognition by Dectin-1. Our studies also indicate that the blockage of GPI anchor synthesis is a strategy to inhibit C. albicans evading host recognition.
Thioredoxin-Interacting-Protein [TXNIP] and Transglutaminase 2 [TGM2] Expression in Meningiomas of Different Grades and the Role of Their Expression in Meningioma Recurrence and Prognosis

Background: Meningiomas are common central nervous system (CNS) tumors that account for thirty percent of primary intracranial tumors. The accuracy of predicting meningioma recurrence and progression is not enough. So, there is a real need for discovering recent factors for identification of the relapse risk, progression rates, which patients will need aggressive treatment and predicting and improving patients’ survival. Thioredoxin-interacting-protein [TXNIP] is an alpha-arrestin-protein family member that is mapped on chromosome 1-q21–22 and is found to participate in cellular redox reactions regulations and control. Transglutaminase 2 (TGM2) is a transglutaminase enzyme family member that is found in many human cells, it may act as an enzyme, a structural protein and also has multiple roles in many cellular activities. Aim of our study: It was to explore the expression of TXNIP, TGM2 and Ki-67 using immunohistochemistry in different pathological grades of meningiomas, and to investigate the relevance between their expressions, clinicopathological criteria, disease recurrence and prognosis of meningioma patients. Methods: we included 50 cases of meningioma of different pathological grades; all patients were managed according to their grade by surgery alone, with radiotherapy or combined modalities. Sections from paraffin blocks prepared from samples of all patients stained by TXNIP, TGM2 and Ki-67 using immunohistochemistry. Results: high expression of TXNIP in 28 out of 50 (56%) cases of meningioma of different pathological grades and was positively correlated with meningioma lower grade, low KI labeling index (p=0.000), adequacy of resection, negatively correlated with high incidence of recurrence after surgery and it was negatively correlated with meningioma higher pathological grades (p=0.000). We detected high expression of TGM2 in 21 out of 50 (42%) cases of meningioma and it was positively correlated with meningioma higher grade (p= 0.002), high KI labeling index (p=0.000), high incidence of recurrence after surgery, progression to higher pathological grades and was negatively correlated with adequacy of resection of meningioma (p=0.000). Conclusion: There is inverse relation between both [TXNIP and TGM2 expression in meningiomas and the combination of decreased expression of TXNIP and increased expression of TGM2 could predict risk of meningioma recurrence and progression in to higher pathological grades.
Diabetes and Coronary Heart Disease in American Indians: The Strong Heart Study

Coronary heart disease (CHD) is the leading cause of death among American Indians. However, information on the prevalence of CHD and its association with known risk factors is limited. The purpose of the Strong Heart Study is to quantify CHD and its risk factors among three geographically diverse groups of American Indians. The population consists of 4,549 adults between 45 and 74 years of age in 13 Indian communities in Arizona, Oklahoma, and South and North Dakota. The phase I examination (1989–1991) revealed very high prevalence rates of diabetes that ranged from 33 to 72% in men and women in the three centers. Prevalence rates of definite myocardial infarction (MI) and definite CHD were higher in men than in women in all three centers (P < 0.0001) and in those with diabetes (P = 0.002 and P = 0.0003 in women and men, respectively). Diabetes was associated with a relatively greater increase in prevalence of MI (prevalence rate = 3.8 vs. 1.9) and CHD (prevalence rate = 4.6 vs. 1.8) in women than in men. Logistic regression analysis indicated that the prevalence of CHD among American Indians was significantly related to age, diabetes, hypertension, albuminuria, percentage of body fat, smoking, high concentrations of plasma insulin, and low concentrations of HDL cholesterol. Lower prevalence rates of CHD were found in Arizona despite higher rates of diabetes, obesity, hypertension, and albuminuria; these lower rates may be in part related to lower smoking frequency and lower concentrations of total and LDL cholesterol. These findings from the baseline Strong Heart Study examination emphasize the relative importance of diabetes and its associated variables as risk factors for CHD among American Indian populations.
Urban-rural differences in the quality of care for medicare patients with acute myocardial infarction.

BACKGROUND There are urban-rural differences in health care utilization in Kansas. This study was conducted to determine if similar differences exist in the quality of inpatient care provided for patients with acute myocardial infarction (AMI). METHODS All acute care hospitals in the state were stratified into 12 urban, 31 semirural, and 76 rural hospitals according to their location. Data from medical records of 2521 Medicare patients 65 years and older who had survived AMI and were discharged alive from hospitals during an 8-month period in 1994/1995 were abstracted. The measures of the quality of care (quality indicators [QIs]) were the use of aspirin (during hospital stay and at discharge) and the administration of beta-blockers, intravenous (IV) nitroglycerin, heparin, and reperfusion by thrombolytic therapy or primary angioplasty. RESULTS A significantly higher proportion of ideal candidates for the use of aspirin during hospital stay and at discharge, heparin, and IV nitroglycerin received these medications in urban hospitals, and a lower proportion of similar patients received these medications in rural hospitals compared with the patients in semirural hospitals (P<.001). Similar trends in each of the 6 QIs were observed for less than ideal patients (P<.05). Patient age was associated with a relatively poor quality of care in terms of the 6 QIs. Except for the administration of IV nitroglycerine to less than ideal patients, age adjustments did not change the observed urban-rural differences in the QI measures. CONCLUSION Relatively poor quality of care for patients with AMI was provided by rural hospitals where greater opportunity for improvement exists.
Exhaled nitric oxide for monitoring childhood asthma inflammation compared to sputum analysis, serum interleukins and pulmonary function

The level of fractional exhaled nitric oxide (FENO) is significantly elevated in uncontrolled asthma and decreases after anti‐inflammatory therapy. The aim of this prospective study was to analyze the behavior of FENO in the follow‐up and management of the inflammation in asthmatic pediatric patients treated with inhaled corticosteroids (ICS), compared to sputum cellularity, serum interleukins (IL), and pulmonary function. Twenty‐six clinically stable asthmatic children aged from 6 to 18 years, previously treated or not with ICS were included. Following an international consensus (GINA), the patients were submitted to standard treatment with inhaled fluticasone for 3 months according to the severity of the disease. During this period, each patient underwent three assessments at intervals of approximately 6 weeks. Each evaluation consisted of the measurement of FENO, determination of serum interleukins IL‐5, IL‐10, IL‐13, and interferon γ (INF‐γ), spirometry and cytological analysis of spontaneous or induced sputum. A significant reduction in mean FENO and IL‐5, without concomitant changes in FEV1, was observed along the study. There was no significant correlation between FeNO and FEV1 in the three assessments. A significant correlation between FeNO and IL‐5 levels was only observed in the third assessment (r = 0.499, P = 0.025). In most patients, serum IL‐10, IL‐13, and INF‐γ concentrations were undetectable throughout the study. Sputum samples were obtained spontaneously in 11 occasions and in 56 by induction with 3% hypertonic saline solution (success rate: 50.8%), with 39 (69.9%) of them adequate for analysis. Only two of the 26 patients produced adequate samples in the three consecutive evaluations, which impaired the determination of a potential association between sputum cellularity and FeNO levels throughout the study. In conclusion, among the parameters of this study, it was difficult to perform and to interpret the serial analysis of spontaneous or induced sputum. Serum interleukins, which remained at very low or undetectable levels in most patients, were not found to be useful for therapeutic monitoring, except for IL‐5 that seems to present some correlation with levels of FeNO exhaled. Monitoring of the mean FEV1 indicated no significant variations during the treatment, demonstrating that functional stability or the absence of obstruction may not reflect the adequate management of asthma. Serial measurement of FeNO seemed to best reflect the progressive anti‐inflammatory action of ICS in asthma. Pediatr Pulmonol. 2008; 43:134–141. © 2007 Wiley‐Liss, Inc.
Detection of gyrA and parC Mutations Associated with Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of Oligonucleotide Biochip Technology

ABSTRACT An oligonucleotide biochip that specifically detects point mutations in the gyrA and parC genes of Neisseria gonorrhoeae was designed and subsequently evaluated with 87 untreated clinical specimens. The susceptibilities of the N. gonorrhoeae strains were tested to determine the prevalence of ciprofloxacin-resistant strains in Anhui Province, People's Republic of China. Conventional DNA sequencing was also performed to identify mutations in gyrA and parC and to confirm the biochip data. The study demonstrates that all of the point mutations in the gyrA and parC genes of N. gonorrhoeae were easily discriminated by use of the oligonucleotide biochip. Fifteen different alteration patterns involved in the formation of ciprofloxacin resistance were identified by the biochip assay. Double mutations in both Ser91 and Asp95 of the GyrA protein were seen in all nonsensitive isolates. Double mutations in Ser91 and Asp95 of GyrA plus mutation of Glu91 or Ser87 of the ParC protein lead to significant high-level resistance to ciprofloxacin in N. gonorrhoeae isolates. The results obtained by use of the oligonucleotide biochip were identical to those obtained by use of DNA sequencing. In conclusion, the oligonucleotide biochip technology has potential utility for the rapid and reliable identification of point mutations in the drug resistance genes of N. gonorrhoeae.
Incorporation of State-Level Variable Stime-Varying Property into the HMM

In HMM-based pattern recognition, the structure of the HMM is often predetermined according to some prior knowledge. In the recognition process, we usually make our judgment based on the maximum likelihood of the HMM, without considering the time-varying property of state-level variables, which unfortunately may lead to incorrect results. In this paper, we analyze the property of state-level variables in the HMM and show it is possible to significantly enhance the performance of speech recognition systems when using the state-level variable time-varying property. We propose to make use of the distribution of the number of intersecting points (NIPs) of state-level variable trajectories in the recognition process, which achieve 2.7 percent correct improvement to a phoneme classification task on TIMIT speech corpus. We also compare the proposed method and state duration model and draw some empirical conclusions
Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions

Horizontal cells in the mouse retina are of the axon‐bearing B‐type and contribute to the gain control of photoreceptors and to the center‐surround organization of bipolar cells by providing feedback and feedforward signals to photoreceptors and bipolar cells, respectively. Horizontal cells form two independent networks, coupled by dendro‐dendritic and axo‐axonal gap junctions composed of connexin57 (Cx57). In Cx57‐deficient mice, occasionally the residual tracer coupling of horizontal cell somata was observed. Also, negative feedback from horizontal cells to photoreceptors, potentially mediated by connexin hemichannels, appeared unaffected. These results point to the expression of a second connexin in mouse horizontal cells. We investigated the expression of Cx50, which was recently identified in axonless A‐type horizontal cells of the rabbit retina. In the mouse retina, Cx50‐immunoreactive puncta were predominantly localized on large axon terminals of horizontal cells. Electron microscopy did not reveal any Cx50‐immunolabeling at the membrane of horizontal cell tips invaginating photoreceptor terminals, ruling out the involvement of Cx50 in negative feedback. Moreover, Cx50 colocalized only rarely with Cx57 on horizontal cell processes, indicating that both connexins form homotypic rather than heterotypic or heteromeric gap junctions. To check whether the expression of Cx50 is changed when Cx57 is lacking, we compared the Cx50 expression in wildtype and Cx57‐deficient mice. However, Cx50 expression was unaffected in Cx57‐deficient mice. In summary, our results indicate that horizontal cell axon terminals form two independent sets of homotypic gap junctions, a feature which might be important for light adaptation in the retina. J. Comp. Neurol. 523:2062–2081, 2015. © 2015 Wiley Periodicals, Inc.
Financial Dollarization of Households and Firms: Does it Differ?

Using a newly complied and extended database from International Financial Statistics, and applying different panel-regression techniques, this paper documents the evolution of householdsâ and firmsâ dollarization over the past decade. We assess the macroeconomic determinants of dollarization for households and firms and explore differences between high and low-income countries. We find that householdsâ and firmsâ dollarization in loans and deposits are weakly explained by the currency substitution model, except in low income countries, where inflation plays a significant role. Instead, market development variables such as financial deepening, access to external debt and FX finance as well as other market considerations are key to explain the dynamics of deposits and loans dollarization, regardless of the level of income.These factors can account for a significant fraction of the dollarization, but using a variance decomposition model, there is evidence that a non-negligible portion has yet to be explained. This suggests that there are key determinants for household and firm dollarization that are not fully captured by traditional macroeconomic explanatory variables.
An analysis of automatic balance evaluator and rehabilitation mechanism processing

The automatic Balance Evaluator and Rehabilitation (ABER) is a general term that covers all the techniques used to quantify postural control in upright stance in either static or dynamic conditions. Among them, Automatic Balance Evaluator and Rehabilitation is a noninvasive specialized clinical assessment technique used to quantify the central nervous system adaptive mechanisms (sensory, motor and central) involved in the control of posture and balance, both in normal (such as in physical education and sports training) and abnormal conditions (particularly in the diagnosis of balance disorders and in physical therapy and postural reduction). Due to the complex interactions among sensory, motor, and central processes involved in posture and balance, ABER requires different protocols in order to differentiate among the many defects and impairments which may affect the patient's posture control system. Thus, ABER challenges it by using several combinations of visual and support surface stimuli and parameters.
Patterns of disagreement in indicators of state repression

Abstract Until recently, researchers who wanted to examine the determinants of state respect for most specific negative rights (i.e., physical integrity and empowerment rights) needed to rely on data from the CIRI or the Political Terror Scale (PTS). The new Varieties of Democracy (V-Dem) dataset offers scholars a potential alternative to the individual human rights variables from CIRI. We analyze a set of key Cingranelli–Richards (CIRI) Human Rights Data Project and V-Dem negative rights indicators, finding unusual and unexpectedly large patterns of disagreement between the two sets. First, we discuss the new V-Dem dataset by comparing it to the disaggregated CIRI indicators, discussing the history of each project, and describing its empirical domain. Second, we identify a set of disaggregated human rights measures that are similar across the two datasets and discuss each project’s measurement approach. Third, we examine how these measures compare to each other empirically, showing that they diverge considerably across both time and space. These findings point to several important directions for future work, such as how conceptual approaches and measurement strategies affect rights scores. For the time being, our findings suggest that researchers should think carefully about using the measures as substitutes.
Endothelial NOS-Mediated Relaxations of Isolated Thoracic Aorta of the C57BL/6J Mouse: A Methodological Study

Endothelium-dependent relaxations were studied in the thoracic aorta (TA) of the C57BL/6J mouse, a strain used commonly in the generation of genetically altered mice, to clarify some methodological questions. First, we have tested if transcardial perfusion with heparinized Krebs solution before the preparation of the TA may improve in vitro relaxant responses. Carbachol, thrombin, and ATP induced significantly stronger relaxations in TAs prepared from perfused animals than in controls. The effect of sodium nitroprusside (SNP), however, did not change, indicating that the improvement of the endothelium-dependent relaxations after perfusion was not caused by increased reactivity of the vascular smooth muscle to NO. Second, the potential regional differences within the TA were studied. Carbachol relaxed significantly stronger distal than proximal TA segments, whereas the effects of thrombin, ATP, and SNP showed no regional heterogeneity. Third, the relaxant effect of carbachol was partially preserved in TAs of endothelial NOS deficient (eNOS−/−) animals and remained unchanged in the presence of indomethacin, indicating the involvement of an eNOS- and cyclooxygenase-independent mechanism in the mediation of the response. Thrombin and ATP were ineffective in eNOS−/− TAs. Finally, TAs prepared from mice housed in cages equipped with running wheels did not show improved reactivity, indicating that the conventional housing conditions and the consequent sedentary lifestyle of the laboratory mouse do not diminish endothelial function in the TA.
Advancing conservation planning for western chimpanzees using IUCN SSC A.P.E.S.—the case of a taxon-specific database

Even though information on global biodiversity trends becomes increasingly available, large taxonomic and spatial data gaps persist at the scale relevant to planning conservation interventions. This is because data collectors are hesitant to share data with global repositories due to workload, lack of incentives, and perceived risk of losing intellectual property rights. In contrast, due to greater conceptual and methodological proximity, taxon-specific database initiatives can provide more direct benefits to data collectors through research collaborations and shared authorship. The IUCN SSC Ape Populations, Environments and Surveys (A.P.E.S.) database was created in 2005 as a repository for data on great apes and other primate taxa. It aims to acquire field survey data and make different types of data accessible, and provide up-to-date species status information. To support the current update of the conservation action plan for western chimpanzees (Pan troglodytes verus) we compiled field surveys for this taxon from IUCN SSC A.P.E.S. , 75% of which were unpublished. We used spatial modeling to infer total population size, range-wide density distribution, population connectivity and landscape-scale metrics. We estimated a total abundance of 52 800 (95% CI 17 577–96 564) western chimpanzees, of which only 17% occurred in national parks. We also found that 10% of chimpanzees live within 25 km of four multi-national ‘development corridors’ currently planned for West Africa. These large infrastructure projects aim to promote economic integration and agriculture expansion, but are likely to cause further habitat loss and reduce population connectivity. We close by demonstrating the wealth of conservation-relevant information derivable from a taxon-specific database like IUCN SSC A.P.E.S. and propose that a network of many more such databases could be created to provide the essential information to conservation that can neither be supplied by one-off projects nor by global repositories, and thus are highly complementary to existing initiatives.
Utilization-aware dynamic GTS allocation scheme in IEEE 802.15.4

IEEE 802.15. 4 is a standard for low-rate wireless personal area network (LR-WPAN) and specifies medium access control (MAC) sub-layer and physical layer. MAC sub-layer in IEEE 802.15. 4 provides a guaranteed time slot (GTS) to guarantee quality of service for applications requiring low latency or minimum data rate. However, since the maximum number of GTSs allowed in each superframe is limited up to seven in IEEE 802.15. 4, efficient GTS allocation scheme is required to improve utilization of wireless resources and enhance quality of service. In this paper, we propose utilization-aware dynamic GTS allocation scheme for IEEE 802.15. 4 to increase throughput and decrease data transmission latency. To obtain our objective, we introduce states and priorities of devices for GTS allocation. The state is utilized to determine priority of devices. PAN coordinator dynamically allocates GTSs to devices in descending order of their priorities. Performance of the proposed scheme is evaluated by simulation on C++. From the results, we confirm that our proposed scheme has better performance than the conventional IEEE 802.15. 4 in terms of throughput and packet waiting time.