Miracle-dz/UniFAR-FTUs huggingface dataset:
An investigation of methyl tert-butyl ether-induced cytotoxicity and protein profile in Chinese hamster ovary cells

Methyl tert-butyl ether (MTBE) is widely used as an oxygenating agent in gasoline to reduce harmful emissions. However, previous studies have demonstrated that MTBE is a cytotoxic substance that has harmful effects in vivo and in vitro. Although remarkable progress has been made in elucidating the mechanisms underlying the MTBE-induced reproductive toxicological effect in different cell lines, the precise mechanisms remain far from understood. The present study aimed to evaluate whether mammalian ovary cells were sensitive to MTBE exposure in vitro by assessing cell viability, lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content and antioxidant enzyme activities. In addition, the effect of MTBE exposure on differential protein expression profiles was examined by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. MTBE exposure induced significant effects on cell viability, LDH leakage, plasma membrane damage and the activity of antioxidant enzymes. In the proteomic analysis, 24 proteins were demonstrated to be significantly affected by MTBE exposure. Functional analysis indicated that these proteins were involved in catalytic activity, binding, structural molecule activity, metabolic processes, cellular processes and localization, highlighting the fact that the cytotoxic mechanisms resulting from MTBE exposure are complex and diverse. The altered expression levels of two representative proteins, heat shock protein family A (Hsp70) members 8 and 9, were further confirmed by western blot analysis. The results revealed that MTBE exposure affects protein expression in Chinese hamster ovary cells and that oxidative stress and altered protein levels constitute the mechanisms underlying MTBE-induced cytotoxicity. These findings provided novel insights into the biochemical mechanisms involved in MTBE-induced cytotoxicity in the reproductive system.
Inhibition of RNA Recruitment and Replication of an RNA Virus by Acridine Derivatives with Known Anti-Prion Activities

Background Small molecule inhibitors of RNA virus replication are potent antiviral drugs and useful to dissect selected steps in the replication process. To identify antiviral compounds against Tomato bushy stunt virus (TBSV), a model positive stranded RNA virus, we tested acridine derivatives, such as chlorpromazine (CPZ) and quinacrine (QC), which are active against prion-based diseases. Methodology/Principal Findings Here, we report that CPZ and QC compounds inhibited TBSV RNA accumulation in plants and in protoplasts. In vitro assays revealed that the inhibitory effects of these compounds were manifested at different steps of TBSV replication. QC was shown to have an effect on multiple steps, including: (i) inhibition of the selective binding of the p33 replication protein to the viral RNA template, which is required for recruitment of viral RNA for replication; (ii) reduction of minus-strand synthesis by the tombusvirus replicase; and (iii) inhibition of translation of the uncapped TBSV genomic RNA. In contrast, CPZ was shown to inhibit the in vitro assembly of the TBSV replicase, likely due to binding of CPZ to intracellular membranes, which are important for RNA virus replication. Conclusion/Significance Since we found that CPZ was also an effective inhibitor of other plant viruses, including Tobacco mosaic virus and Turnip crinkle virus, it seems likely that CPZ has a broad range of antiviral activity. Thus, these inhibitors constitute effective tools to study similarities in replication strategies of various RNA viruses.
Interpolation of graph signals using shift-invariant graph filters

New schemes to recover signals defined in the nodes of a graph are proposed. Our focus is on reconstructing bandlimited graph signals, which are signals that admit a sparse representation in a frequency domain related to the structure of the graph. The schemes are designed within the framework of linear shift-invariant graph filters and consider that the seeding signals are injected only at a subset of interpolating nodes. After several sequential applications of the graph-shift operator - which computes linear combinations of the information available at neighboring nodes - the seeding signals are diffused across the graph and the original bandlimited signal is eventually recovered. Conditions under which the recovery is feasible are given, and the corresponding schemes to recover the signal are proposed. Connections with the classical interpolation in the time domain are also discussed.
Mashhad PNU Readiness For Rmplementation Of Total Quality Management(TQM)

This study investigated the relationship of total quality management through Mashhad PNU ready to run TQM descriptive and correlational analysis was performed and the data is used. The population consists of 50 directors of Morgan questionnaire was based on a sample of 45 patients were available. A researcher-made questionnaire was Likert format to assess the validity of the diagnostic validity and reliability of Cronbachs alpha was used to measure. Data using the software package (spss. 18) pasw by descriptive statistics and statistical model T single-sample analysis. Results showed a penchant for TQM concept and variables in the PNU coordinated efforts of the management team, participate in hands-on efforts to improve quality, time to spread the philosophy of Total Quality Management, Resources and giving praise and rewards for good in the process of promoting and applying the variable Total Quality Management philosophy in words and deeds more than the average High contrast and bright plans for the future are less variable and lower than average.
Differentiating Clear Channel Assessment Using Transmit Power Variation

Clear Channel Assessment (CCA) is a core element of Wireless Sensor Network (WSN) Medium Access Control (MAC) protocols which is used on the transmitter and receiver sides. Current CCA implementations cannot determine the device type occupying the media, leaving nodes unable to differentiate between WSN traffic and interference. However, this would be valuable as MAC protocols benefit from reacting differently depending on the channel occupier. In this article, we describe a method called Power Differentiating Clear Channel Assessment (P-DCCA). Transmitters vary the output power of the radio while the packet is being sent. Receivers are able to identify signals with this characteristic, enabling a Differentiating Clear Channel Assessment (DCCA) check to reveal if the medium is currently occupied by WSN traffic or other interference. We present an implementation and thorough evaluation of Power P-DCCA. Using ContikiMAC as an example, we describe how P-DCCA can be integrated within MAC protocols. We show via large-scale testbed experiments and deployments that P-DCCA enabled networks have a significant improved performance. For example, we show that a P-DCCA enabled network can improve Packet Reception Rate (PRR) by up to a factor of 10 while reducing energy usage by more than 80% under heavy interference.
Efﬁcacy of Intravenous Iron Sucrose Administration for Correcting Preoperative Anemia in Patients Scheduled for Major Orthopedic Surgery

the use of intravenous sucrose for the treatment of iron anemia in found a mean maximum in hemoglobin (1.0 (cid:1) g/dl) start of intravenous iron that administration of intravenous iron 2–3 weeks may be optimal. We regarding the patients’ inclusion criteria, the dosage and administration schedule of iron sucrose, and the comparison of their data with those of the study by Cuenca et al. 2 in patients sustaining a pertrochanteric hip fracture.
Experimental implementation of flocking algorithms in wheeled mobile robots

In this paper we present an experimental implementation of the flocking algorithm presented by H.G. Tanner et al., (2003) for the double integrator model, in the case of wheeled mobile robots (WMR). We use the look ahead concept to feedback linearize the WMR model and obtain its double integrator form in which we apply the control signals in the form of voltages in wheel motors. A virtual leader is used to steer the flock in the desired direction. Comparisons of the experimental results with simulation verify the robustness of this algorithm to model uncertainties and communication delays.
Genotype phenotype correlation in Wilson's disease within families--a report on four south Indian families.

AIM To study the genotype phenotype correlation in Wilson's disease (WD) patients within families. METHODS We report four unrelated families from South India with nine members affected with WD. Phenotype was classified as per international consensus phenotypic classification of WD. DNA was extracted from peripheral blood and 21 exons of ATP7B gene and flanking introns were amplified by polymerase chain reaction (PCR). The PCR products were screened for mutations and the aberrant products noted on screening were sequenced. RESULTS Four separate ATP7B mutations were found in the four families. ATP7B mutations were identical amongst affected members within each family. Three families had homozygous mutations of ATP7B gene while one family had compound heterozygous mutation, of which only one mutation was identified. We noted concordance between ATP7B gene mutation and Wilson's disease phenotype amongst members within each family. The age of onset of symptoms or of detection of asymptomatic disease, baseline serum ceruloplasmin and baseline urinary copper levels were also similar in affected members of each family. Minor differences in phenotype and baseline serum ceruloplasmin level were noted in one family. CONCLUSION We report concordance between ATP7B mutation and WD phenotype within each family with > 1 member affected with WD. Homozygous ATP7B mutation was present in 3 of the 4 families studied. Our report supports allelic dominance as a determinant of WD phenotype. However, in one family with compound heterozygous mutation, there was a similar WD phenotype which suggests that there may be other factors determining the phenotype.
Extending a Lexicon Ontology for Intelligent Information Integration

One of the current research on the Semantic Web area is semantic annotation of information sources. On-line lexical ontologies can be exploited as a-priori common knowledge to provide easily understandable, machine-readable metadata. Nevertheless, the absence of terms related to specific domains causes a loss of semantics. In this paper we present WNEditor, a tool that aims at guiding the annotation designer during the creation of a domain lexicon ontology, extending the pre-existing WordNet ontology. New terms, meanings and relations between terms are virtually added and managed by preserving the WordNet's internal organization.
Fault diagnosis of rolling element bearings from current and vibration measurements

As rolling element bearings are used in many rotary machines, it is crucial to monitor their response in order to apply condition-based maintenance. One of the main tasks consists in carrying out the diagnosis process, i.e., to do fault detection, localization and identification. Thus, the replacement of rolling element bearings can be done once the diagnosis process gives relevant information that is not only valid for maintenance purposes, but also to design purposes when the causes of the faults appearing in the system are analysed. There are many ways to study the state of these components, which vary from vibration analysis until acoustic emissions, through current or thermal analysis, among others.In this work the vibrational response of rolling element bearings as well as the current of the motor that drives the motion of these components are analysed. A test rig consisting of a drive motor, a couple of gearboxes and a load motor is used. Damaged bearings have been used in the first gearbox, in such a way that their response is studied and compared to that of a healthy bearing. Some indicators are extracted from both the vibration and current signals that can be used for diagnosis purposes. Moreover, the effect of other factors such as the size of the damage or the position of the damaged zone on the vibrational and electrical response is also studied.
Development of Useful Recombinant Promoter and Its Expression Analysis in Different Plant Cells Using Confocal Laser Scanning Microscopy

Background Designing functionally efficient recombinant promoters having reduced sequence homology and enhanced promoter activity will be an important step toward successful stacking or pyramiding of genes in a plant cell for developing transgenic plants expressing desired traits(s). Also basic knowledge regarding plant cell specific expression of a transgene under control of a promoter is crucial to assess the promoter's efficacy. Methodology/Principal Findings We have constructed a set of 10 recombinant promoters incorporating different up-stream activation sequences (UAS) of Mirabilis mosaic virus sub-genomic transcript (MS8, -306 to +27) and TATA containing core domains of Figwort mosaic virus sub-genomic transcript promoter (FS3, −271 to +31). Efficacies of recombinant promoters coupled to GUS and GFP reporter genes were tested in tobacco protoplasts. Among these, a 369-bp long hybrid sub-genomic transcript promoter (MSgt-FSgt) showed the highest activity in both transient and transgenic systems. In a transient system, MSgt-FSgt was 10.31, 2.86 and 2.18 times more active compared to the CaMV35S, MS8 and FS3 promoters, respectively. In transgenic tobacco (Nicotiana tabaccum, var. Samsun NN) and Arabidopsis plants, the MSgt-FSgt hybrid promoter showed 14.22 and 7.16 times stronger activity compared to CaMV35S promoter respectively. The correlation between GUS activity and uidA-mRNA levels in transgenic tobacco plants were identified by qRT-PCR. Both CaMV35S and MSgt-FSgt promoters caused gene silencing but the degree of silencing are less in the case of the MSgt-FSgt promoter compared to CaMV35S. Quantification of GUS activity in individual plant cells driven by the MSgt-FSgt and the CaMV35S promoter were estimated using confocal laser scanning microscopy and compared. Conclusion and Significance We propose strong recombinant promoter MSgt-FSgt, developed in this study, could be very useful for high-level constitutive expression of transgenes in a wide variety of plant cells.
Mortality in Patients With Pararenal Aortic Pathologies Treated With Chimney or Fenestrated Endografts

clinically relevant embolic events during peripheral interventions is low, and most of these events can be successfully managed during the index procedure, as shown by the study of Shrikhande and colleagues and Dieter and Najundappa. However, nothing is known about the clinical consequences of initially silent distal embolic events in patients undergoing repetitive redo procedures. In particular, patients at high risk for such redo procedures are the ones who have the highest peri-interventional embolic risk: long lesions, chronic total occlusions, and instent reocclusions. For clinicians, it is a well known phenomenon that peripheral outflow is progressively compromised in stepwise fashion with every additional complex procedure. Notably, the clinical implication of this finding is still uncertain. Considering the low incidence of clinically meaningful consequences of peri-interventional embolic events, it is very unlikely that such a largescale trial evaluating the clinical benefit of any kind of embolic prevention device will be conducted in the near future. However, as mentioned in our article, there exists other meaningful endpoints that might be significantly affected by the use of protection devices, such as radiation time, amount of contrast potentially affecting renal function in patients with renal insufficiency, and, finally, treatment time, and with this catheterization lab time. We definitely agree with Wu and colleagues that a sufficiently powered, hard endpoint trial is mandatory before the general use of the ECA balloon can be recommended. Nevertheless, everything is a matter of cost: if the cost of an ECA balloon is not significantly higher than a 30cm-long standard angioplasty balloon, the ECA balloon could be at least considered currently in lesions at high risk for distal embolization.
Congestion management in Indian Power Transmission System

Background/Objectives: Study of biometric for human identification is trending nowadays. Several methods and algorithms have been suggested for the usage in biometric recognition system. â€œA biometric can be based on either a personâ€™s physical or behavioural characteristics. â€ The key approach behind the means is to uniquely recognize humans by their inherent physical characteristics. Recognition or identification through these biometric features dissipates the issues related with the traditional approaches for identifyinghumans. Humans are identified or recognized through their physical characteristics and not by any external entity that they must provide for the process. Methodology: The paper presents a short review of all the methods used for the biometric identification using lips by various people. The various papers have been read and studied. The algorithms were analyzed. Then the methods have been implemented to show the output. Results/Findings: Biometric identification such as lip prints is used to recognize every individual.Lip shape and features have been detected using various algorithms.
The analgesic efficacy of intravenous lidocaine infusion after laparoscopic fundoplication: a prospective, randomized, double-blind, placebo-controlled trial

This study aimed to determine if intravenous lidocaine infusion reduces postoperative pain intensity following laparoscopic fundoplication surgery and to also validate the safety of intravenous lidocaine at the dose tested. This was an equally randomized, double-blind, placebo-controlled, parallel-group, single center trial. Adult patients undergoing laparoscopic fundoplication were recruited. The intervention group received 1 mg/kg intravenous lidocaine bolus prior to induction of anesthesia, then an intravenous infusion at 2 mg/kg/h for 24 hours. The primary outcome was pain, measured using a numeric rating scale for 30 hours postoperatively. Secondary outcomes were nausea and vomiting, opioid requirements, adverse events, serum lidocaine concentration, and length of hospital stay. The study was terminated after an interim analysis of 24 patients showed evidence of futility. There was no difference in postoperative pain scores (lidocaine versus control, mean ± standard deviation) at rest (2.0 ± 2.7 vs 2.1 ± 2.4, P=0.286) or with movement (2.0 ± 2.6 vs 2.6 ± 2.7, P=0.487). Three adverse events occurred in the lidocaine group (25% of patients). Intravenous lidocaine did not provide clinically significant analgesia to patients undergoing laparoscopic fundoplication. The serum lidocaine concentration of patients who experienced adverse events were within the therapeutic range. This trial cannot confirm the safety of intravenous lidocaine at the dose tested.
The distribution of natural fractures and joints at depth in crystalline rock

This paper presents the results of studies of the natural fracture distribution encountered in 10 test wells drilled in three areas of the United States. Seven of the wells were drilled to depths of 200–250 m, while three were drilled to depths of about 1 km. Using an ultrasonic borehole televiewer, fracture depths, strikes, and dips were determined. Steeply dipping fractures were found throughout each of the wells, and in general, few horizontal fractures were observed. Statistically significant fracture pole concentrations were found for each well which were basically invariant with depth, although some variation of fracture orientation with depth was found in two wells. The significant fracture orientations were not found to be the same in wells only several kilometers apart in a given region. In none of the wells did the number of observable fractures decrease markedly with increasing depth. No simple relationship of fracture orientation or fracture density with major structural features such as the San Andreas fault were observed, and no simple relation between the significant fracture orientations and either past or present regional stress fields could be determined.
SobolevFusion: 3D Reconstruction of Scenes Undergoing Free Non-rigid Motion

We present a system that builds 3D models of non-rigidly moving surfaces from scratch in real time using a single RGB-D stream. Our solution is based on the variational level set method, thus it copes with arbitrary geometry, including topological changes. It warps a given truncated signed distance field (TSDF) to a target TSDF via gradient flow. Unlike previous approaches that define the gradient using an L2 inner product, our method relies on gradient flow in Sobolev space. Its favourable regularity properties allow for a more straightforward energy formulation that is faster to compute and that achieves higher geometric detail, mitigating the over-smoothing effects introduced by other regularization schemes. In addition, the coarse-to-fine evolution behaviour of the flow is able to handle larger motions, making few frames sufficient for a high-fidelity reconstruction. Last but not least, our pipeline determines voxel correspondences between partial shapes by matching signatures in a low-dimensional embedding of their Laplacian eigenfunctions, and is thus able to reliably colour the output model. A variety of quantitative and qualitative evaluations demonstrate the advantages of our technique.
VAR Management to Improve Maximum Loading In IEEE 30 Bus System Using FACTS Controllers

The maximum loading of the transmission lines depends upon various limits. These are related to the environmental conditions, length of the line and various stability related issues. The VAR plays important role in maximising the power transfer through a transmission line. The FACTS Controllers have a major part to play in the VAR Management, in order to increase the maximum loading capability. This gives an additional benefit of enhancing the voltage stability margin of the entire system as well. In this paper this aspect of VAR Management, is investigated for an IEEE 30 Bus Test System incorporating various FACTS Controllers like TCSC, STATCOM and SVC.
Phenolic Compounds from the Leaves of Castanopsis fargesii

In the course of a phytochemical and chemotaxonomical investigation of Castanopsis species (Fagaceae), three new phenolic compounds, (3R,1′S)-[1′-(6″-O-galloyl-β-d-gluco-pyranosyl)oxyethyl]-3-hydroxy-dihydrofuran-2(3H)-one (1), (2R,3S)-2-[2′-(galloyl)oxyethyl]-dihydroxybutanoic acid (2), and (3S,4S)-3-hydroxymethyl-3,4-dihydro-5,6,7-trihydroxy-4-(4′-hydroxy-3′-methoxyphenyl)-1H-[2]-benzopyran-1-one (3) were isolated from the fresh leaves of Castanopsis fargesii. In addition, a known phenolic glycoside, gentisic acid 5-O-α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranoside (4) was also isolated and identified. Their structures were elucidated by means of spectroscopic methods including one- and two-dimensional NMR techniques.
Aggregation in Aqueous Solutions of Bovine Lens y-Crystallins : Special Role of ys

RESULTS. All of the y-crystallins studied formed large aggregates (or "megamers") in aqueous solutions. However, each protein differed in the relative rates of formation of megamers. ysCrystallin formed megamers much more slowly than y,,and yIVa-crystallin. In solutions containing mixtures of y,, and ys, and y,Va and ys, ys-crystallin significantly suppressed the aggregation of y,,and yIV:i-crystallin. Megamerization seemed to be associated with thiol oxidation in these proteins.