Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of key genes associated with Schmid-type metaphyseal chondrodysplasia based on microarray data

This study aimed to gain a better understanding of the molecular circuitry of Schmid-type metaphyseal chondrodysplasia (SMCD), and to identify more potential genes associated with the pathogenesis of SMCD. Microarray data from GSE72261 were downloaded from the NCBI GEO database, including collagen X p.Asn617Lys knock-in mutation (ColXN617K), ablated XBP1 activity (Xbp1CartΔEx2), compound mutant (C/X), and wild-type (WT) specimens. Differentially expressed genes (DEGs) were screened in Xbp1 vs. WT, Col vs. WT and CX vs. WT, respectively. Pathway enrichment analysis of these DEGs was performed. Transcription factors (TFs) of the overlapping DEGs were identified. Weighted correlation network analysis (WGCNA) was performed to find modules of DEGs with high correlations, followed by gene function analysis and a protein-protein interaction network construction. In total, 481, 1,530 and 1,214 DEGs were identified in Xbp1 vs. WT, Col vs. WT and CX vs. WT, respectively. These DEGs were enriched in different pathways, such as extracellular matrix (ECM)-receptor interaction and metabolism-related pathways. A total of 7 TFs were found to regulate 19 common upregulated genes, and 4 TFs were identified to regulate 21 common downregulated genes. Two significant gene co-expression modules were enriched and DEGs in the 2 modules were mainly enriched in different biological processes, such as ribosome biogenesis. Moreover, Kras (downregulated), Col5a1 (upregulated) and Furin (upregulated) were both identified in the regulatory networks and protein-protein interaction (PPI) network. On the whole, our findings indicate that the Kras, Col5a1 and Furin genes may play essential roles in the molecular mechanisms of SMCD, which warrants further investigation.
Hybrid control approach of CLL resonant converter for EV battery chargers

In this study, a CLL resonant converter with different control strategies; frequency, dc-link, and phase shift control are compared and a hybrid control is proposed to increase the efficiency of the system for Electric Vehicle (EV) battery charger applications. The comparison analysis is examined considering the battery charging control algorithms constant current (CC) and constant voltage (CV) with modeling the battery as a variable load. In order to improve charging efficiency, the system should operate in a close to resonant frequency, achieving ZVS and reducing circulating current. The presented control is achieved by staying around the resonant frequency in all charging conditions. The designed dc/dc resonant converter, fed by a power-factor-corrector (PFC) with 80-120 V output, provides 120 V / 10 A at 1.2 kW maximum power. The application results show that the proposed hybrid control improves overall efficiency 2 %.
A novel ZVZCS phase-shifted full-bridge converter with secondary-side energy storage inductor used for electric vehicles

This paper presents a novel ZVZCS phase-shift full-bridge (PSFB) DC-DC converter with secondary-side energy storage inductor, which can be utilized in high voltage application such as electric vehicle. By employing an energy storage inductor and an output capacitive filter at the secondary side, there is little reverse recovery loss in output-diodes due to relatively slow downslope of the triangular current. Meanwhile, the voltage spike across the output diode can be also eliminated and clamped by the output capacitor directly. Moreover, the proposed converter introduces a passive auxiliary circuit to satisfy the ZVS operation of the converter from no-load to full-load conditions resulting in superior efficiency in very wide load variations. Finally, the operational principle and analysis of the proposed converter are presented in detail. Experimental results provided from a 1kW prototype verify the feasibility and superior performance of the proposed converter.
Data Sharing in Psychology

Routine data sharing, defined here as the publication of the primary data and any supporting materials required to interpret the data acquired as part of a research study, is still in its infancy in psychology, as in many domains. Nevertheless, with increased scrutiny on reproducibility and more funder mandates requiring sharing of data, the issues surrounding data sharing are moving beyond whether data sharing is a benefit or a bane to science, to what data should be shared and how. Here, we present an overview of these issues, specifically focusing on the sharing of so-called “long tail” data, that is, data generated by individual laboratories as part of largely hypothesis-driven research. We draw on experiences in other domains to discuss attitudes toward data sharing, cost-benefits, best practices and infrastructure. We argue that the publishing of data sets is an integral component of 21st-century scholarship. Moreover, although not all issues around how and what to share have been resolved, a consensus on principles and best practices for effective data sharing and the infrastructure for sharing many types of data are largely in place.
Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis

Psychrotroph microorganisms have developed cellular mechanisms to cope with cold stress. Cell envelopes are key components for bacterial survival. Outer membrane is a constituent of Gram negative bacterial envelopes, consisting of several components, such as lipopolysaccharides (LPS). In this work we investigated the relevance of envelope characteristics for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis by analyzing a mini Tn5 wapH mutant strain, encoding a core LPS glycosyltransferase. Our results showed that wapH strain is impaired to grow under low temperature but not for cold survival. The mutation in wapH, provoked a strong aggregative phenotype and modifications of envelope nanomechanical properties such as lower flexibility and higher turgor pressure, cell permeability and surface area to volume ratio (S/V). Changes in these characteristics were also observed in the wild type strain grown at different temperatures, showing higher cell flexibility but lower turgor pressure under cold conditions. Cold shock experiments indicated that an acclimation period in the wild type is necessary for cell flexibility and S/V ratio adjustments. Alteration in cell-cell interaction capabilities was observed in wapH strain. Mixed cells of wild type and wapH strains, as well as those of the wild type strain grown at different temperatures, showed a mosaic pattern of aggregation. These results indicate that wapH mutation provoked marked envelope alterations showing that LPS core conservation appears as a novel essential feature for active growth under cold conditions.
Development of sintering process hard metals to wear resistant efficiency on sustainable material management

This study aims to development of production process of hard metal by tungsten carbide powder with the particle sized of 0.1 0.35 μm. This carbide powder were made of recycling cemented carbide tool scraps, so it is mixed with Ta = 2.82 % , Mo = 0.45 % , Nb = 1.22 % , Ti = 4.56 % , Zr = 0.37 % The shape of the carbide powder are roughly round due to ball milling for 300 hours. It was mixing cobalt 10 % and Paraffin wax 2 %, which is found the work piece that running through the process of sintering with a temperature of 1,400 °c and 1,430 °c , have the hardness of 1,368.5 and 1,346.3 HV, respectively. In one hour of holding time process, the work piece with 1,430 °c of the sintering causes less wear. After the test of turning operation, it can be concluded that the tungsten carbide cutting tool provides the equivalent quality as a standard cutting tool.
[CONSORT for reporting randomized controlled trials in journal and conference abstracts: explanation and elaboration].

BACKGROUND Clear, transparent, and sufficiently detailed abstracts of conferences and journal articles related to randomized controlled trials (RCTs) are important, because readers often base their assessment of a trial solely on information in the abstract. Here, we extend the CONSORT (Consolidated Standards of Reporting Trials) Statement to develop a minimum list of essential items, which authors should consider when reporting the results of a RCT in any journal or conference abstract. METHODS AND FINDINGS We generated a list of items from existing quality assessment tools and empirical evidence. A three-round, modified-Delphi process was used to select items. In all, 109 participants were invited to participate in an electronic survey; the response rate was 61%. Survey results were presented at a meeting of the CONSORT Group in Montebello, Canada, January 2007, involving 26 participants, including clinical trialists, statisticians, epidemiologists, and biomedical editors. Checklist items were discussed for eligibility into the final checklist. The checklist was then revised to ensure that it reflected discussions held during and subsequent to the meeting. CONSORT for Abstracts recommends that abstracts relating to RCTs have a structured format. Items should include details of trial objectives; trial design (e.g., method of allocation, blinding/masking); trial participants (i.e., description, numbers randomized, and number analyzed); interventions intended for each randomized group and their impact on primary efficacy outcomes and harms; trial conclusions; trial registration name and number; and source of funding. We recommend the checklist be used in conjunction with this explanatory document, which includes examples of good reporting, rationale, and evidence, when available, for the inclusion of each item. CONCLUSIONS CONSORT for Abstracts aims to improve reporting of abstracts of RCTs published in journal articles and conference proceedings. It will help authors of abstracts of these trials provide the detail and clarity needed by readers wishing to assess a trial's validity and the applicability of its results.
Clinical and histopathological characteristics in patients with scarring folliculitis type of acne inversa

ABSTRACT Objective: This study was designed to study the clinical and histopathological characteristics of patients with the scarring folliculitis type acne inversa in Chinese population. Methods: A total of 21 patients with acne inversa and 6 controls without known dermatological disease were recruited from outpatient department of dermatology and orthopedic surgery. Two-millimeter punch biopsies were taken from 8 patients with acne inversa and 6 controls, fixed in formalin, embedded in paraffin and stained with haematoxylin and eosin prior to histopathological analysis. Results: There were 12 patients (57.14%) belonging to the scarring folliculitis type presented with double comedones, papules, nodules, depressed scars, and were mainly Hurley stage I (66.67%). Many of the scarring folliculitis type were smokers (58.33%), some had a history of occupational exposure (41.67%) and some were overweight (50%), the mean BMI of which is 25.18±3.16 kg/m2. Histopathological changes such as perifollicular inflammation can be observed in scarring folliculitis type of acne inversa and controls as well. However, epidermal hyperplasia, follicular hyperplasia, sebaceous gland disappearance, destruction of hair follicle and sebaceous gland, collagen hyperplasia, perivascular inflammation, granulomatous inflammation, Micro thrombus were only seen in scarring folliculitis type. The mean surface area in patients (8073.36±15798.43 μm2) was smaller than that in controls (302059.08±502813.78 μm2), with statistically significant difference. (P = 0.024). Conclusion: The scarring folliculitis type in acne inversa in Chinese population could be characterized by depressed scars, double-ended comedones, epidermal cysts and had high proportion of smokers, or occupational exposure with lower Hurley stage, as well as diminished sebaceous gland. Further studies are needed to clarify the relations between the clinical subtypes of acne inversa and their corresponding genetypes.
Surfactant Protein D in Respiratory and Non-Respiratory Diseases

Surfactant protein D (SP-D) is a multimeric collectin that is involved in innate immune defense and expressed in pulmonary, as well as non-pulmonary, epithelia. SP-D exerts antimicrobial effects and dampens inflammation through direct microbial interactions and modulation of host cell responses via a series of cellular receptors. However, low protein concentrations, genetic variation, biochemical modification, and proteolytic breakdown can induce decomposition of multimeric SP-D into low-molecular weight forms, which may induce pro-inflammatory SP-D signaling. Multimeric SP-D can decompose into trimeric SP-D, and this process, and total SP-D levels, are partly determined by variation within the SP-D gene, SFTPD. SP-D has been implicated in the development of respiratory diseases including respiratory distress syndrome, bronchopulmonary dysplasia, allergic asthma, and chronic obstructive pulmonary disease. Disease-induced breakdown or modifications of SP-D facilitate its systemic leakage from the lung, and circulatory SP-D is a promising biomarker for lung injury. Moreover, studies in preclinical animal models have demonstrated that local pulmonary treatment with recombinant SP-D is beneficial in these diseases. In recent years, SP-D has been shown to exert antimicrobial and anti-inflammatory effects in various non-pulmonary organs and to have effects on lipid metabolism and pro-inflammatory effects in vessel walls, which enhance the risk of atherosclerosis. A common SFTPD polymorphism is associated with atherosclerosis and diabetes, and SP-D has been associated with metabolic disorders because of its effects in the endothelium and adipocytes and its obesity-dampening properties. This review summarizes and discusses the reported genetic associations of SP-D with disease and the clinical utility of circulating SP-D for respiratory disease prognosis. Moreover, basic research on the mechanistic links between SP-D and respiratory, cardiovascular, and metabolic diseases is summarized. Perspectives on the development of SP-D therapy are addressed.
Aging Adventure Athletes Assess Achievements and Alter Aspirations to Maintain Self-Esteem

Achievements and capabilities influence the self-esteem of skilled adventure athletes. Self-esteem affects individual mental health. Aging commonly reduces adventure capabilities. To avoid loss in self-esteem, aging adventure athletes are forced to adjust their aspirations. Here, I examine this process using participant observation, ethnographic and autoethnographic approaches. The qualitative data for this analysis are derived from 60 years’ experience in outdoor adventure activities, and ∼30,000 person-hours of participant observation. I argue that individuals assess their own capabilities against a set of specific feats. For some activities, successful completion of a specific feat is known as nailing it. The selection of these feats depends on factors such as activity and geographic location, as well as individual experience and peer comparisons. I examine the detailed process using a single feat repeated over a period of decades, the bubble-line kayak run through Lava Falls on the Grand Canyon of the Colorado River. I compare other examples of nail or fail to construct a general framework for self-esteem in aging adventure athletes, with both physical and psychological feedback loops. I also identify two key thresholds, as aging adventure athletes recognize their declining skills. These may apply to aging more broadly, beyond outdoor adventure.
Advanced laboratory for testing plasma thrusters and Hall thruster measurement campaign

Abstract Plasma engines are used for space propulsion as an alternative to chemical thrusters. Due to the high exhaust velocity of the propellant, they are more efficient for long-distance interplanetary space missions than their conventional counterparts. An advanced laboratory of plasma space propulsion (PlaNS) at the Institute of Plasma Physics and Laser Microfusion (IPPLM) specializes in designing and testing various electric propulsion devices. Inside of a special vacuum chamber with three performance pumps, an environment similar to the one that prevails in space is created. An innovative Micro Pulsed Plasma Thruster (LμPPT) with liquid propellant was built at the laboratory. Now it is used to test the second prototype of Hall effect thruster (HET) operating on krypton propellant. Meantime, an improved prototype of krypton Hall thruster is constructed.
An Update on in Vivo Imaging of Extracellular Vesicles as Drug Delivery Vehicles

Extracellular vesicles (EVs) are currently being considered as promising drug delivery vehicles. EVs are naturally occurring vesicles that exhibit many characteristics favorable to serve as drug delivery vehicles. In addition, EVs have inherent properties for treatment of cancers and other diseases. For research and clinical translation of use of EVs as drug delivery vehicles, in vivo tracking of EVs is essential. The latest molecular imaging techniques enable the tracking of EVs in living animals. However, each molecular imaging technique has its certain advantages and limitations for the in vivo imaging of EVs; therefore, understanding the molecular imaging techniques is essential to select the most appropriate imaging technology to achieve the desired imaging goal. In this review, we summarize the characteristics of EVs as drug delivery vehicles and the molecular imaging techniques used in visualizing and monitoring EVs in in vivo environments. Furthermore, we provide a perceptual vision of EVs as drug delivery vehicles and in vivo monitoring of EVs using molecular imaging technologies.
A quantitative analysis of quorum system availability in data centers

Large-scale distributed storage systems often replicate data across servers and even geographically-distributed data centers for high availability, while existing theories like CAP and PACELC show that there is a tradeoff between availability and consistency. However, current practice is mainly experience-based and lacks quantitative analysis for identifying a good tradeoff between the two. In this work, we are concerned with providing a quantitative analysis on availability for widely-used quorum systems in data centers. First, a probabilistic model is presented to quantify availability for typical data center networks: 2-tier basic tree, 3-tier basic tree, fat tree and folded clos network. Second, we build the availability-consistency table and propose a set of rules to quantitatively make tradeoff between availability and consistency. Finally, with Monte Carlo based simulations, we validate our presented quantitative results and show that our approach to make tradeoff between availability and consistency is effective.
Protective features of resveratrol on human spermatozoa cryopreservation may be mediated through 5’ AMP‐activated protein kinase activation

Biochemical and physical modifications during the freeze–thaw process adversely influence the restoration of energy‐dependent sperm functions required for fertilization. Resveratrol, a phytoalexin, has been introduced to activate 5′ AMP‐activated protein kinase which is a cell energy sensor and a cell metabolism regulator. The cryoprotection of resveratrol on sperm cryoinjury via activation of AMP‐activated protein kinase also remains to be elucidated. Our aim, thus, was to investigate: (i) the presence and intracellular localization of AMP‐activated protein kinase protein; (ii) whether resveratrol may exert a protective effect on certain functional properties of fresh and post–thaw human spermatozoa through modulation of AMP‐activated protein kinase. Spermatozoa from normozoospermic men were incubated with or without different concentrations of Compound C as an AMP‐activated protein kinase inhibitor or resveratrol as an AMP‐activated protein kinase activator for different lengths of time and were then cryopreserved. AMP‐activated protein kinase is expressed essentially in the entire flagellum and the post‐equatorial region. Viability of fresh spermatozoa was not significantly affected by the presence of Compound C or resveratrol. However, although Compound C caused a potent inhibition of spermatozoa motility parameters, resveratrol did not induce negative effect, except a significant reduction in motility at 25 μm for 1 h. Furthermore, resveratrol significantly increased AMP‐activated protein kinase phosphorylation and mitochondrial membrane potential and decreased reactive oxygen species and apoptosis‐like changes in frozen–thawed spermatozoa. Nevertheless, it was not able to compensate decreased sperm viability and motility parameters following cryopreservation. In contrast, Compound C showed opposite effects to resveratrol on AMP‐activated protein kinase phosphorylation, reactive oxygen species, apoptosis‐like changes, mitochondrial membrane potential, and motility parameters. These findings, although preliminary, suggest that resveratrol‐induced improvement of cryopreserved sperm functions may be mediated through activation of AMP‐activated protein kinase, indicating the importance of AMP‐activated protein kinase activity for human spermatozoa functions. Further investigations are required to elucidate the mechanism by which resveratrol ameliorates oxidative stress‐mediated damages in an AMP‐activated protein kinase‐dependent mechanism.
Effects of Covering Surgical Wounds with Polyglycolic Acid Sheets for Posttonsillectomy Pain

Postoperative pain is a remaining issue in tonsillectomy. Polyglycolic acid (PGA) is a biocompatible material used for absorbent suture reinforcement, and its sheet has been applied for covering defects after resection of oral carcinoma. The aim of this study is to examine whether the attachment of a PGA sheet to surgical wounds would reduce posttonsillectomy pain. In this prospective single-blind study, 17 consecutive adult patients were recruited who needed to undergo tonsillectomy, mainly due to habitual tonsillitis. Following bilateral tonsillectomies, a PGA sheet was attached with fibrin glue to only 1 side, without notification to patients of which side. Postoperative pain of each side was separately evaluated with a visual analog scale at 4 time points: before each meal and before sleep. Postoperative pain of both the PGA sheet–attached and nonattached sides was most severe before breakfast among 4 time points. Postoperative pain measured before breakfast was significantly more severe in the PGA sheet–attached side than the nonattached side. As such, this study provided solid data on the negative effects of PGA sheeting on posttonsillectomy pain.