Miracle-dz/UniFAR-FTUs huggingface dataset:
The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo.

BACKGROUND Previous results from our laboratory have led us to propose heparan sulfate (HS) as a putative protamine acceptor during human sperm decondensation in vivo. The aim of this paper was to investigate the presence of glycosaminoglycans in the mammalian oocyte in an effort to better support this contention. METHODS Two experimental approaches are used: oocyte labeling to identify the presence of HS and analysis of sperm decondensing ability of fresh oocytes in the presence or absence of specific glycosidases. RESULTS Staining of mouse zona-intact oocytes with the fluorescent cationic dye, Rubipy, at pH 1.5 allowed for the detection of sulfate residues in the ooplasm by confocal microscopy. HS was detected in the ooplasm by immunocytochemistry. A sperm decondensation microassay using heparin and glutathione was successfully developed. The same level of sperm decondensation could be attained when heparin was replaced by mouse zona-free oocytes. Addition of heparinase to the oocyte/glutathione mixture significantly reduced sperm decondensation (P = 0.0159), while there was no effect following addition of either chondroitinase ABC or hyaluronidase. CONCLUSIONS The results presented in this paper demonstrate for the first time that HS is present in the mammalian oocyte and show that HS is necessary for fresh oocytes to express their sperm decondensing ability in vitro.
Molecular analysis of transmission of hepatitis C virus in a nurse who acquired acute hepatitis C after caring for a viremic patient with epistaxis

A 23‐year‐old nurse (HC‐IP) developed acute hepatitis C. Intrafamilial transmission of hepatitis C virus (HCV) was suspected initially because her parents were carriers of HCV of the same genotype (1b) as that of Patient HC‐IP. However, the HCV isolate from Patient HC‐IP and those from her parents shared identities of only 92.4–92.7% in the 1,087‐nucleotide (nt) sequence within the NS5B region. It was then suspected that she contracted HCV infection during medical practice. Sixteen patients with antibodies to HCV (anti‐HCV) were hospitalized 1–3 months before she became positive for anti‐HCV. Upon analysis of stored serum samples, 14 of the 16 patients were found to be positive for HCV RNA, and 9 of the 14 viremic patients had genotype 1b HCV. Although the shared identities between the HCV isolate from Patient HC‐IP and those from eight of the nine patients were merely 90.6–93.9% within the 1,087‐nt NS5B sequence, the HCV isolate from the remaining one patient (HC‐P12) was 99.7% identical to that from Patient HC‐IP. Upon analysis of the E1 and E2 junctional region including hypervariable region 1 (283 nt), there was a close relationship (99.3–100%) between clones obtained from Patients HC‐IP and HC‐P12. Although the nurse HC‐IP had a finger injury, she took care of Patient HC‐P12, a 70‐year‐old man with HCV‐related cirrhosis and recurrent epistaxis, occasionally without wearing protective gloves. This study indicates the occurrence of HCV transmission by exposure of nonintact skin to blood in health care settings. J. Med. Virol. 81:1363–1370, 2009. © 2009 Wiley‐Liss, Inc.
Comparison of updated Vitek Yeast Biochemical Card and API 20C yeast identification systems

The updated Vitek Yeast Biochemical Card (YBC) was compared with the API 20C by using 409 germ tube-negative yeasts and Geotrichum spp. that were either clinical or proficiency sample isolates. The API 20C was the reference standard. The 409 isolates represented nine genera and 21 species. Morphology agars were inoculated and interpreted for each isolate. The API 20C identified 406 isolates (99.3%), while the Vitek YBC identified 367 (89.7%). Both systems identified the majority of yeasts after 24 h of incubation--73.4% were identified by the API 20C and 77.4% were identified by the Vitek YBC. The Vitek 24-h reading had some incorrect identifications. These included 14 isolates of Candida tropicalis that were identified as Candida parapsilosis (91 to 97% reliability) and 3 isolates of Candida krusei that were called Blastoschizomyces capitatus (Geotrichum capitatum), Candida rugosa, and Candida zeylanoides. In total, the Vitek YBC misidentified 30 isolates, while the API 20C misidentified 3 isolates. In addition, results for 14 isolates with the Vitek YBC were listed under the category "no identification. " Morphology agars were required for identification with 89 isolates (21.9%) when the API 20C was used and with 50 isolates (12.6%) when the Vitek YBC was used. Apart from the price of the Vitek instrument, the API 20C costs $1.28 more per test than the Vitek YBC. Overall, the updated Vitek YBC compares favorably with the API 20C in the identification of common yeasts such as Torulopsis glabrata, C. parapsilosis, and Cryptococcus neoformans. However, problems were encountered with the Vitek system in the identification of C. tropicalis, C. krusei, Trichosporon spp. , and some Cryptococcus spp. The routine use of morphology agars with either method is recommended.
Analytical Determination of the Aerosol Organic Mass-to-Organic Carbon Ratio

Abstract Particulate matter (PM) with an aerodynamic diameter ≤2.5 μm (PM2.5) was collected daily (mid-July 1998 to the end of December 1999) over a 24-hr sampling period in a mixed light industrial-residential area in Atlanta, GA, to provide a subset of data for the Aerosol Research and Inhalation Epidemiology Study (ARIES). This study included the measurement of organic carbon (OC), elemental carbon (EC), and individual organic compounds. OC and EC average mean concentrations were 4.50 ± 0.33 and 2.08 ± 0.19 μg/m3, respectively. The ratio of organic matter mass (OM) to OC in PM2.5 aerosols in Atlanta was measured using three different approaches: (1) solvent extract residue gravimetric masses to individual OC concentrations of sequential apolar to polar solvent extracts (dichloromethane, acetone, and water); (2) mass balance of the PM2.5 measured gravimetric mass minus the mass concentrations of the inorganic/elemental constituents to the total OC concentration; and (3) polar organic compound speciation with the concentration weighted ratio to the total OC concentration. We found very good agreement between approach 1 and 2. The average OM/OC ratio calculated from the extract residue mass was 2.14 ± 0.17. The average OM/OC ratio determined by mass balance was 2.16 ± 0.43 for the whole period. The concentration weighted ratio calculated from the concentrations of polar organic compounds ranged between 1.55 and 1.72, which was likely a lower limit for the ratio because of the limited number of the polar organic compounds that can be quantified using gas chromatographic methods. We found seasonal differences with an OM/OC range of 1.77 in December 1999 to 2.39 in July 1999. These results suggest that the previously accepted value of 1.4 for the OM/OC ratio was too low even for urban locations during the winter months. Molecular-level speciation of the PM2.5-associated organic compounds showed that the concentrations of the molecular markers for wood smoke represented approximately 12–15% of the total polar organic compound concentrations during the winter months
Does the Political Party in the Government Increase Intentional Homicide in Brazil?

This paper investigates the impacts of the Workersâ€™ Party (WP) in government on the homicide rate in Brazil from panel data for the Brazilian states between 1980 and 2011 using the system of generalized method of moments. An important explanatory factor in the homicide rate is the association between socioeconomic variables and left political parties in state governments. The results provide empirical evidence that the WP's control of the government increased the homicide rate more than the other political parties. This suggests that increasing the number of years of WP occupation of the presidency leads to a more pronounced increase in the homicide rate, compared with other political parties. Although the observed trend indicates increased rates of homicide, more substantial growth was observed in almost all states under WP rule during the period 2003 to 2011, contributing to a higher overall level of violence in Brazil.
Do people adapt to climate change? Evidence from the industrialized countries

PurposeThis study aims to explore the link between mortality and climate change. The focus is in particular on individuals’ adaptation to temperature changes. The authors analyze the relationship between climatic change (measured by temperature rate) and mortality in 23 Organisation for Economic Co-operation and Development countries during 1970-2010.Design/methodology/approachThis study performs the adaptation regression model in the level form as a dynamic panel fixed effects model. The authors use a non-linear threshold estimation approach to examine the extreme temperature changes effect on the temperature–mortality relation. More specifically, the study explores whether the large increases/decreases in temperature rates affect mortality rates more than the modest changes.FindingsThis study indicates that the temperature–mortality relation is significant in early part of the sample period (before 1990) but insignificant during the second part (after 1990). After including controlling factors, as well as nation and year fixed effects, the authors provide evidence that people do adapt to the most of the temperature-related mortalities. Also, this study provides evidence of the non-linear relationship between national temperatures and mortality rates. It is observed that only after 5 per cent increase in the annual temperature, the relation between temperature and overall mortality is significant.Originality/valueMost studies cover only one specific country, hence making it difficult to generalize across countries. Therefore, the authors argue that the best estimation of the health effects of temperature change can be found by modeling the past relationships between temperature and mortality across countries for a relatively long period. To the authors’ knowledge, previous studies have not systemically tested the adaptation effect across countries.
Importance of Friendship Potential in Reducing the Negative Effects of Dissimilarity

The purpose of this study was to examine the influence of friendship potential on perceptions of dissimilarity among persons in diverse groups. The author gathered data at the beginning and end of a 15-week semester from college students enrolled in physical activity classes. Structural equation modeling indicated that friendship potential was negatively related to perceptions of deep-level dissimilarity at the end of the semester. This relation was moderated by perceptions of such dissimilarity at the beginning of the semester. The author found the perceived dissimilarity to be negatively associated with affective reactions to the class. The author discusses findings in terms of contributions and implications for diverse groups.
Shock Propagation in Cascade Systems: Analysis and Applications

In this paper, we study shock propagation in cascade systems that can be a model of outage of power systems and that of spreading a fad. While existing research works have dealt with a certain relationship between global cascade and system properties, such as degree and distribution of threshold values, and interconnection topology, this paper addresses new engineering problems of the cascade systems. Mainly, we would like to 1) identify subsystems contributing to a large cascade, 2) develop a strategy that prevents all initial failures within a designated region from cascading out of the region, and 3) develop a strategy that prevents all initial failures outside of the region from cascading into the region. In order to solve these challenging problems, we develop an invariant set analysis method for cascade models and derive some features inherent to the cascade systems. As the first feature, supposing that we know the resulting cascade region from the given initial failures in specific subsystems, we will show that the cascading of initial failures of any combination of the subsystems cannot exceed the region. The second feature is a graph theoretic distance condition, which guarantees that two cascading regions are never combined together. Based on the analytical results, we develop algorithms solving the aforementioned engineering problems, in which the complexity of the computation is reduced.
Crypto-Securities Regulation: ICOs, Token Sales and Cryptocurrencies under EU Financial Law

Cryptocurrencies, such as bitcoin and ethereum, have not only risen to public attention as novel means of payments, but also as facilitators of initial coin offerings (ICOs, also called token sales). In these entirely online-mediated offerings, entrepreneurs sell tokens registered on a blockchain in exchange for cryptocoins. Buyers receive tokens that can be understood as cryptographically-secured coupons which embody a bundle of rights and obligations. In July 2017, the SEC released an investigative report that highlighted that such tokens can be subject to the full scope of US securities regulation. It is unclear, however, to what extent EU securities regulation is applicable to ICOs and, particularly, whether issuers have to publish and register a prospectus in order to avoid criminal and civil prospectus liability in the EU. In conceptual terms, this depends on whether tokens are considered “securities” under the EU prospectus regulation regime. Against this background, this paper develops a nuanced approach that distinguishes between three archetypes of tokens: currency, investment, and utility tokens. It analyzes the differential implications of each of these types, and their hybrid forms, for EU securities regulation, and develops policy proposals for their regulation.
After the Storm

In this study, we explored the experiences of 13 individuals who had suffered an electrical injury at work and had subsequently returned to work. In this article, we report on the social, institutional, and relational elements that workers perceived to influence return to work experiences and the provision of workplace accommodations. These elements included (a) worker resources, (b) job characteristics, (c) workplace setting, (d) injury elements, (e) workers’ compensation context, and (f) supports and advocacy provided. We conclude that the availability and provision of supportive accommodations are influenced by a multiplicity of interrelated factors including the legitimacy of resulting impairments following electrical injury, institutional structures (e.g., compensation and health care systems), the social relations of work, and broader labor market and economic contexts. Those workers who were vulnerable because of factors such as employment circumstances or labor market conditions were often poorly supported when returning to work following electrical injury.
On the Effectiveness of Mode Exploration in Bayesian Model Averaging for Neural Networks

Multiple techniques for producing calibrated predictive probabilities using deep neural networks in supervised learning settings have emerged that leverage approaches to ensemble diverse solutions discovered during cyclic training or training from multiple random starting points (deep ensembles). However, only a limited amount of work has investigated the utility of exploring the local region around each diverse solution (posterior mode). Using three well-known deep architectures on the CIFAR-10 dataset, we evaluate several simple methods for exploring local regions of the weight space with respect to Brier score, accuracy, and expected calibration error. We consider both Bayesian inference techniques (variational inference and Hamiltonian Monte Carlo applied to the softmax output layer) as well as utilizing the stochastic gradient descent trajectory near optima. While adding separate modes to the ensemble uniformly improves performance, we show that the simple mode exploration methods considered here produce little to no improvement over ensembles without mode exploration.
Electronic dispersion compensation for PMD in 40-GB/s optical links

We analyzed the performance of DFE in PMD-limited 40 GB/s Optical links by using Matlab/Simulink. We also provided a description of the equalizer circuit design, and described the results of circuit simulations; it is found that a DFE consisting of a 3-tap FFE and a 2-tap FBE could compensate PMD.
How Intrinsic Motivation can Speed Up Language Emergence

Natural languages enable humans to engage in highly complex social and conversational interactions with each other. Alife approaches to the origins and emergence of language typically manage this complexity by carefully staging the learning paths that embodied artificial agents need to follow in order to bootstrap their own communication system from scratch. This paper investigates how these scaffolds introduced by the experimenter can be removed by allowing agents to autonomously set their own challenges when they are driven by intrinsic motivation and have the capacity to self-assess their own skills at achieving their communicative goals. The results suggest that intrinsic motivation not only allows agents to spontaneously develop their own learning paths, but also that they are able to make faster transitions from one learning phase to the next.
A digitally controlled DC-DC converter for SoC in 28nm CMOS

Battery operation in mobile applications needs power efficient DC-DC converters which are able to handle battery voltages up to 5.5V. Normally, these DC-DC converters are built in special technologies. For decreased footprint and chip count of the overall system, a system-on-chip solution on modern CMOS technology with core supply voltages around 1V is preferred. The presented DC-DC buck converter generates 0.9 to 1.8V at output currents up to 500mA from a battery voltage of 2.4 to 5.5V with high efficiency in a high-k 28nm metal-gate CMOS technology.
Considering Water Quality of Urban Rivers from the Perspectives of Unpleasant Odor

Surface water degradation in urban areas is a common problem in many countries, and degradation hampers ecosystem services provided by rivers, having negative impacts on aquatic organisms and human beings. Unpleasant odor arising from impaired rivers causes annoyance to people, and the study quantitatively analyzed unpleasantness of odor in Tokyo through the application of hedonic tones of odor. Unpleasantness remained or worsened while overall dissolved oxygen (DO) concentration increased downstream. The phenomenon is considered to be caused by multiple external factors, such as combined sewer outflow, hypoxia at Tokyo Bay, and effluent from water reclamation centers (WRC). Converting descriptive odor types into numeric values helped to see changes over time and enabled to depict the relation between DO concentration and the type of odor. In Tokyo, the ratio of effluent from WRC to stream flow increased over time, and it partially contributed to odor becoming more unpleasant. Malodor is no longer a major issue since the situation improved by developing an advanced treatment facility; however, human olfactory sense detects the existence of unpleasant odor in urban rivers. The study presented the complexity of analyzing odor problems in tidal urban areas because these are caused by external factors.
Pyrolytic carbon resurfacing arthroplasty for osteoarthritis of the proximal interphalangeal joint of the finger.

BACKGROUND Pyrolytic carbon resurfacing arthroplasty of the proximal interphalangeal joint has been reported to provide favorable results. The purpose of this study was to evaluate the effectiveness of pyrolytic carbon joint replacement at an average follow-up of fifty-five months. METHODS A retrospective review of thirty-one arthroplasties of the proximal interphalangeal joint performed by a single surgeon in seventeen patients with interphalangeal joint osteoarthritis was conducted. Assessment included range of motion, patient satisfaction, and pain scores. Radiographs were evaluated for alignment, implant subsidence, and implant failure. The Michigan Hand Outcomes Questionnaire was administered at the time of the final follow-up. RESULTS The average follow-up period was fifty-five months (minimum, two years). No patient was lost to follow-up. The arc of motion of the interphalangeal joint decreased from 57° preoperatively to 31° (p < 0.05) at the time of the final follow-up. The average score for pain was 3 of 10 on a visual analog scale. Satisfaction averaged 3.4 points on a 5-point Likert scale, and twelve of seventeen patients stated that they would repeat the surgery. Data from the Michigan Hand Outcomes Questionnaire were compared for the involved and noninvolved hands of the fifteen patients who received implants unilaterally. Significant deficits were observed in the total outcome score, activities of daily living, function, and satisfaction for the operatively treated hand. Pain was also greater in the operatively treated hand. Complications included implant fracture (one joint), dislocation (five joints), squeaking (eleven), loosening (fifteen), and interphalangeal joint contracture (twenty). Six joints required a reoperation (an arthrodesis in four joints, a silicone arthroplasty in one, and excision of exostosis in one). Implant migration was severe for seven proximal phalanx implants and three distal phalanx implants, and one implant breached the phalangeal cortex. CONCLUSIONS Interphalangeal joint motion decreased significantly at the final follow-up evaluation, following short-term gains in the initial postoperative period. Complications were numerous, and implant loosening with migration was a major problem. Arthroplasty of the proximal interphalangeal joint with a pyrolytic carbon implant has a high complication rate, poor outcomes, and variable patient satisfaction. On the basis of these findings, we no longer use this implant in our practice.
Post-traumatic cerebral infarction: Neuroimaging findings, etiology and outcome

Purpose: To assess the radiological characteristics of post-traumatic cerebral infarctions (PTCIs), the etiology and site of infarction, and to provide neuroimaging indicators of a poor clinical outcome. Material and Methods: A retrospective study of 16 patients with the neuroimaging-based diagnosis of PTCI was carried out. All CT, MR examinations, cerebral angiograms and medical records of the patients were reviewed. Results: Infarcts were diagnosed in the territory of the posterior cerebral artery in 9 patients, in the middle cerebral artery in 5, in the anterior cerebral artery in 3, lenticulostriate-thalamoperforating in 2, vertebrobasilar in 3, and cortical infarcts in 2 patients. Neuroimaging studies suggested focal mass effect and/or acquired intracranial herniations as the cause of infarction in 13/16 patients (81.2%). In 3/16 patients (18.8%), PTCI was due to vascular injury of which 2 were angiographically documented (carotid artery dissection). Eight of the 16 patients in this study died or were left in a persistent vegetative state. Patients with associated subdural hematoma, brain swelling/edema and traumatic subarachnoid hemorrhage (tSAH) exhibited the worst outcome. Conclusion: Gross mechanical shift of the brain and herniation across the falx and/or tentorium accounted for infarction in a majority of cases in our study. The overall death rate was 43.8% and this result suggests that PTCI is an indication of a poor clinical outcome, especially among patients with associated subdural hematoma, brain swelling/edema and tSAH.