Miracle-dz/UniFAR-FTUs huggingface dataset:
Analysis of the Ribosomal Protein S19 Interactome*S

Ribosomal protein S19 (RPS19) is a 16-kDa protein found mainly as a component of the ribosomal 40 S subunit. Its mutations are responsible for Diamond Blackfan anemia, a congenital disease characterized by defective erythroid progenitor maturation. Dysregulation of RPS19 has therefore been implicated in this defective erythropoiesis, although the link between them is still unclear. Two not mutually exclusive hypotheses have been proposed: altered protein synthesis and loss of unknown functions not directly connected with the structural role of RPS19 in the ribosome. A role in rRNA processing has been surmised for the yeast ortholog, whereas the extracellular RPS19 dimer has a monocyte chemotactic activity. Three proteins are known to interact with RPS19: FGF2, complement component 5 receptor 1, and a nucleolar protein called RPS19-binding protein. We have used a yeast two-hybrid approach to identify a fourth protein: the serine-threonine kinase PIM1. The present study describes our use of proteomics strategies to look for proteins interacting with RPS19 to determine its functions. Proteins were isolated by affinity purification with a GST-RPS19 recombinant protein and identified using LCMS/MS analysis coupled to bioinformatics tools. We identified 159 proteins from the following Gene Ontology categories: NTPases (ATPases and GTPases; five proteins), hydrolases/helicases (19 proteins), isomerases (two proteins), kinases (three proteins), splicing factors (five proteins), structural constituents of ribosome (29 proteins), transcription factors (11 proteins), transferases (five proteins), transporters (nine proteins), DNA/RNA-binding protein species (53 proteins), other (one dehydrogenase protein, one ligase protein, one peptidase protein, one receptor protein, and one translation elongation factor), and 13 proteins of still unknown function. Proteomics results were validated by affinity purification and Western blotting. These interactions were further confirmed by co-immunoprecipitation using a monoclonal RPS19 antibody. Many interactors are nucleolar proteins and thus are expected to take part in the RPS19 interactome; however, some proteins suggest additional functional roles for RPS19.
The Shifting Sands of Counseling Psychology Programs’ Institutional Contexts

Despite strong indicators of the robustness of counseling psychology, the specialty seems somewhat vulnerable, as evidenced by the fact that several solid programs have been forced to phase out or to convert to combined-integrated programs. In fact, analyzing the trends among counseling psychology programs reveals that 30% of programs that have been accredited have been phased out. This article provides a context for understanding the accreditation history of counseling psychology programs. We then offer solutions and strategies to reduce this particular vulnerability. The proximal and distal solutions that are presented may help ensure the continued flourishing of counseling psychology across institutional, programmatic, and national levels.
Genetic and Developmental Control of Nuclear Accumulation of COP1, a Repressor of Photomorphogenesis in Arabidopsis

Using a [beta]-glucuronidase (GUS) reporter-COP1 fusion transgene, it was shown previously that Arabidopsis COP1 acts within the nucleus as a repressor of seedling photomorphogenic development and that light inactivation of COP1 was accompanied by a reduction of COP1 nuclear abundance (A.G. von Arnim, X.-W. Deng [1994] Cell 79: 1035–1045). Here we report that the GUS-COP1 fusion transgene can completely rescue the defect of cop1 mutations and thus is fully functional during seedling development. The kinetics of GUS-COP1 relocalization in a cop1 null mutant background during dark/light transitions imply that the regulation of the functional nuclear COP1 level plays a role in stably maintaining a committed seedling's developmental fate rather than in causing such a commitment. Analysis of GUS-COP1 cellular localization in mutant hypocotyls of all other pleiotropic COP/DET/FUS loci revealed that nuclear localization of GUS-COP1 was diminished under both dark and light conditions in all mutants tested, whereas nuclear localization was not affected in the less pleiotropic cop4 mutant. Using both the brassinosteroid-deficient mutant det2 and brassinosteroid treatment of wild-type seedlings, we have demonstrated that brassinosteroid does not control the hypocotyl cell elongation through regulating nuclear localization of COP1. The growth regulator cytokinin, which also dramatically reduced hypocotyl cell elongation in the absence of light, did not prevent GUS-COP1 nuclear localization in dark-grown seedlings. Our results suggest that all of the previously characterized pleiotropic COP/DET/FUS loci are required for the proper nuclear localization of the COP1 protein in the dark, whereas the less pleiotropic COP/DET loci or plant regulators tested are likely to act either downstream of COP1 or by independent pathways.
The Use of Computer-Assisted Instruction as an Instructional Tool to Teach Social Stories to Individuals Who Have Been Diagnosed on the Autism Spectrum

This article discusses the use of computer-assisted instruction (CAI) to teach Social Stories as a method of positively affecting the social understanding and behaviors of learners who have been diagnosed on the autism spectrum. As the diagnosis rate for those with ASD continues to rise along with the practice of including these learners in the general education environment, there is an increasing need to identify evidence-based practices that focus on the acquisition and remediation of social-communication skills, social skills, and social competence. A variety of strategies have been developed and implemented to remediate deficits in these areas. The use of CAI is an approach that has been proven to accommodate both the needs and the visual learning styles of these learners.
A new variance stabilizing transformation for gene expression data analysis

Abstract In this paper, we introduce a new family of power transformations, which has the generalized logarithm as one of its members, in the same manner as the usual logarithm belongs to the family of Box-Cox power transformations. Although the new family has been developed for analyzing gene expression data, it allows a wider scope of mean-variance related data to be reached. We study the analytical properties of the new family of transformations, as well as the mean-variance relationships that are stabilized by using its members. We propose a methodology based on this new family, which includes a simple strategy for selecting the family member adequate for a data set. We evaluate the finite sample behavior of different classical and robust estimators based on this strategy by Monte Carlo simulations. We analyze real genomic data by using the proposed transformation to empirically show how the new methodology allows the variance of these data to be stabilized.
Overflow of catecholamine neurotransmitters to the circulation: source, fate, and functions.

Article de synthese sur l'efflux des neurotransmetteurs catecholaminergiques chez les mammiferes. Origine des catecholamines: la noradrenaline, l'adrenaline et la dopamine dans le plasma et l'urine. Decharge de ces catecholamines dans le corps entier et dans les differents organes. Etude des determinants de la decharge de la noradrenaline des nerfs sympathiques vers la circulation. Utilisation des mesures du taux de decharge dans l'estimation du mode de decharge des nerfs
Simple coherent receivers for partial response continuous phase modulation

By using a pulse-amplitude-modulation representation of binary continuous-phase-modulation signals, the authors develops a novel optimum Viterbi sequence detector and a near-optimum Viterbi receiver with low complexity. For modulation index 0.5, where a linear receiver can be used, a minimum-mean-squared-error linear receiver filter is derived. The performance of all of these is analyzed, using the Gaussian minimum-shift-keying signal (GMSK) for illustration. It is shown that a GMSK receiver consisting of two matched filters and a four-state Viterbi algorithm performs with less than 0.24-dB degradation compared with the optimal receiver. The linear receiver is optimum for all values of E/sub b//N/sub 0/ (bit-energy-to-noise one-sided spectral density ratio). A design method for its filter is given. The filter is equivalent to a cascade of a matched filter and a Wiener filter estimator. Both upper and lower bounds for the bit-error probability are calculated. Simulation results which confirm the analysis are given. >
Encountering Pedagogy through Relational Art Practices

Two artists involved in ‘socially engaged art’ practice were invited to work with art education teacher candidates and instructors in an effort to rethink notions of teaching, learning and art. We initiated this residency, which we called ‘The Summerhill Residency’, to examine how learning encounters might create environments for meaningful exchanges between the ways in which artists and secondary art education teacher candidates learn to think about pedagogy and the nature of artistic learning. Drawing upon Bourriaud's theory of relational aesthetics, we consider, yet trouble, the relational aspects of the processes and products of the artist residency, and examine the crisis of imagination that permeated teacher candidates' experiences. Throughout the project, a/r/tography offered a rich form of living enquiry that opened up possibilities for learning within a community of enquirers.
Speaker-adaptation in a hybrid HMM-MLP recognizer

Presently the most important systems for large vocabulary, continuous speech recognition are speaker-independent. These systems deal with the inter-speaker variability through a large pool of speakers. However, this approach has several drawbacks due to its inability to cope with the individual speaker characteristics. The problem is more extreme for the cases of fast or non-native speakers. In this paper we present a technique for speaker-adaptation in the context of a hybrid HMM-MLP system for large vocabulary, speaker-independent, continuous speech recognition. This technique is implemented both in supervised and unsupervised modes. In the unsupervised case both static and incremental approaches are explored. The results show that speaker-adaptation within the hybrid HMM-MLP framework can substantially improve system performance. In the incremental unsupervised mode, the improvement is obtained without any extra demands on the speaker, i.e. without an enrolment phase.
Multidrug resistance and extended-spectrum β-lactamases genes among Escherichia coli from patients with urinary tract infections in Northwestern Libya

Introduction Multidrug resistance (MDR) and emergence of extended-spectrum β-lactamases (ESBLs) that mediate resistance to β-lactam drugs among Escherichia coli and other uropathogens have been reported worldwide. However, there is little information on the detection of ESBLs genes in E. coli from patients with urinary tract infections (UTIs) in the Arab countries using polymerase chain reaction (PCR), and in Libya such information is lacking. Methods All patients attending Zawiya Teaching Hospital in Zawiya city between November 2012 and June 2013 suspected of having UTIs and from whom midstream urine samples were taken as part of the clinical workup were included in this prospective study. Samples were examined for uropathogens by standard bacteriological procedures. VITEK-2 automated microbiology system was used to identify the isolated uropathogens and determine the susceptibility of E. coli and Klebsiella spp. isolates to antimicrobials. In addition, phenotypically ESBLs-positive E. coli isolates were tested for ESBLs genes by PCR. Results The present study enrolled 1,790 patients with UTIs. Uropathogens were found in 371 (20.7%) urine specimens examined. Mixed pathogens were detected in two specimens with 373 total pathogens isolated. E. coli and Klebsiella spp. were the predominant uropathogens at 55.8% (208/373) and 18.5% (69/373), respectively. Other pathogens were detected in 25.7% (96/373) of urine samples. Of the E. coli and Klebsiella spp. tested, 69.2 and 100% were resistant to ampicillin, 6.7 and 33.3% to ceftriaxone, and 23.1 and 17.4% to ciprofloxacin, respectively. MDR (resistance to ≥3 antimicrobial groups) was found in 69 (33.2%) of E. coli and in 29 (42%) of Klebsiella spp. isolates. ESBLs were detected phenotypically in 14 (6.7%) of E. coli and in 15 (21.7%) of Klebsiella spp. isolates. Thirteen out of the 14 phenotypically ESBL-positive E. coli were positive for ESBL genes by PCR. blaTEM gene was detected in seven isolates, blaOXA gene in 10 isolates and blaCTX-M gene in six isolates. blaSHV gene was not detected in the present study. Conclusion The isolation of MDR ESBL-producing uropathogens undoubtedly will limit the choices clinicians have to treat their patients with UTIs. Therefore, there is an urgent need for surveillance studies on antimicrobial resistance and prevalence of ESBLs among uropathogens to guide the clinical treatment of UTIs in Libya in the future.
2-Aminopyrimidinium nitrate

In the title compound, C4H6N3 +·NO3 −, the cation is coplanar with the anion (r.m.s. deviation = 0.048 Å), and links to the anion via an N—H⋯O hydrogen bond, forming an ion pair. In the crystal, adjacent ion pairs are further linked by N—H⋯O hydrogen bonds into linear chains running along the b axis.
Neisseria conserved hypothetical protein DMP12 is a DNA mimic that binds to histone-like HU protein

DNA mimic proteins are unique factors that control the DNA-binding activity of target proteins by directly occupying their DNA-binding sites. To date, only a few DNA mimic proteins have been reported and their functions analyzed. Here, we present evidence that the Neisseria conserved hypothetical protein DMP12 should be added to this list. Our gel filtration and analytical ultracentrifugation results showed that the DMP12 monomer interacts with the dimeric form of the bacterial histone-like protein HU. Subsequent structural analysis of DMP12 showed that the shape and electrostatic surface of the DMP12 monomer are similar to those of the straight portion of the bent HU-bound DNA and complementary to those of HU protein dimer. DMP12 also protects HU protein from limited digestion by trypsin and enhances the growth rate Escherichia coli. Functionally, HU proteins participate in bacterial nucleoid formation, as well as recombination, gene regulation and DNA replication. The interaction between DMP12 and HU protein might, therefore, play important roles in these DNA-related mechanisms.
Short-Term and Midterm Load Forecasting Using a Bilevel Optimization Model

During the last decade, neural networks have emerged as one of the most powerful and accurate nonlinear models for load forecasting. However, using neural networks requires users to have in-depth knowledge to determine the model structure and parameters, which limits their wide application. To overcome this weakness, this paper proposes an integrated approach which combines a self-organizing fuzzy neural network (SOFNN) learning method with a bilevel optimization method. SOFNNs can automatically determine both the model structure and parameters, while the bilevel optimization method automatically selects the best pre-training parameters to ensure that the best fuzzy neural networks be identified. Therefore, the proposed approach is able to automatically identify the best fuzzy neural network for a given forecasting task and is much easier to use in practice. The proposed approach is tested on real-load data from the Southern Power Network of Hebei Province, China, and on the EUNITE competition data. Results show the proposed approach improves existing load forecasting models.
First report of Klebsiella pneumonia carbapenemase-producing Pseudomonas aeruginosa isolated from burn patients in Iran: phenotypic and genotypic methods

Wound infection associated with carbapenem-resistant Pseudomonas aeruginosa in burn patients is a growing problem. One of the main mechanisms of resistance to carbapenem antibiotics is the ability of P. aeruginosa to produce carbapenemase enzymes. Klebsiella pneumonia carbapemenase (KPC) is an important type of carbapenemase which can hydrolyze carbapenem antibiotics. The Modified Hodge Test (MHT) and boronic acid as a KPC inhibitor are two phenotypic methods used for detection of carbapenemase. The sensitivity and specificity of these two phenotypic tests for the identification of KPC can be measured by PCR. In this study, 241 P. aeruginosa strains were isolated from wounds of hospitalized burn patients. Carbapenem-resistant P. aeruginosa isolates were determined by the disk diffusion method. KPC-producing carbapenem-resistant strains were examined using the Modified Hodge Test, followed by boronic acid. Further, strains with positive responses to MHT and boronic acid tests were analyzed with the PCR molecular method. One hundred eighty-six of 241 isolates were resistant to carbapenems and 75 were positive in the MHT. Three exhibited an at least 5-mm diameter difference when meropenem was combined with boronic acid vs meropenem alone in the boronic acid test. Two strains had a specific band with primer No.1 after gel electrophoresis. This study showed that MHT, despite excellent sensitivity, has variable specificity independent of bacterial species. Further, the use of KPC inhibitors such as boronic acid did not yield favorable sensitivity and specificity among the specimens from Iranian patients. Thus, it seems that sequencing after PCR should be considered the gold standard for the detection of KPC-producing P. aeruginosa.
Social integration in youth: Insights from a social network perspective

Research repeatedly demonstrates the detrimental consequences of social isolation, but little is known of why adolescents lack social integration. Therefore, the present study uses social network analyses to take a unique look at adolescents’ degree of integration. A total of 317 seventh through ninth graders (13.7 years, 162 girls) out of 18 classes reported friendship patterns and their respective class teachers provided information on students’ social skills. Results showed that social-skill indicators improve with higher categories of integration. Furthermore, in two-level-random-intercept models (L1: adolescents, L2: peer groups), indicators of social skills predicted the degree of integration each adolescent had on both the individual and the contextual level. Findings support the importance of adaptive peer relationships, because the degree to which adolescents are socially integrated in class is not merely related to their own but also to their friends’ social-psychological constitution.
Employee Reactions to a Reduction in Benefit-Related Compensation: A Fairness Perspective

Many organizations are shifting the financial burden of increased costs of medical benefits to employees. The effects of the changes in benefits on employee outcomes are not fully understood. The present study examined how employees reacted to a reduction in benefit-related compensation and how potential negative reactions can be minimized. Data from 229 employees showed that preference for how medical benefits should be distributed (equality versus need-based distributions) as well as past utilization of benefits were related to perceptions of distributive organizational justice. Perceptions of procedural organizational justice were predicted by employee perceptions of how well the benefit changes were communicated and by past utilization of benefits. Providing additional information to employees about changes in benefits, and providing employee voice, increased employee perceptions of informational justice.
The relation of the anger subcomponent of Type A behavior to psychological symptoms in Japanese and foreign students

The relations of the Type A behavior subcomponents to psychological symptoms were examined. Participants were 429 Japanese college students and 307 foreign students. Type A behavior was measured by the Everyday Behavior Questionnaire, developed in part from several rating scales and composed of four subcomponents: anger/impatience, speed, competitiveness/hard-driving, and eagerness/interest. The Hopkins Symptom Checklist was used to measure psychological symptoms. The results of multiple regression analyses on the Type A behavior subcomponents and psychological symptoms indicated strong positive relations between anger/impatience and psychological symptoms, in both Japanese and foreign students. It was concluded that the Type A subcomponents anger and impatience seemed to worsen the psychological health of individuals of various cultural backgrounds.
Estimating some labour market implications of skill biased technology change and imports in Hungary

This paper examines the influence of capital accumulation and imports on the relative demand for skilled and unskilled labour in Hungary between 1980 and 2002. Historical data shows that both relative employment and wages of skilled labour were favourably influenced by the changes of the past decades, when the transformation of the Hungarian economy from a centrally planned to a market led regime took place. The paper studies whether these effects were similar to those observed in the international literature in the context of developed and less developed economies. Besides facilitating international comparisons, examination of factor demand functions also aims to complement existing results from the Hungarian literature.
An in-kernel NOSQL cache for range queries using FPGA NIC

To make use of big data, various NOSQL data stores have been deployed, such as key-value stores and column-oriented stores. NOSQL data stores typically achieve a high degree of scalability, while specialized for some specific purposes; thus, Polyglot persistence that employs multiple NOSQL data stores complementally is a practical choice toward a high diversity of application demands. We assume various NOSQL data stores running on database servers are accessed by clients via a network. This paper aims to improve performance of the Polyglot persistence by introducing an FPGA-based 10GbE network interface (NIC) and In-Kernel NOSQL Cache (IKNC) implemented in the NIC device driver. IKNC stores query results as a key-value form in a host memory, and the requested data can be returned to clients immediately if the query result has been cached. In the IKNC keyvalue pair, the key is a hashed value of a given search query and the value is a query result of the search query. Existing works have focused only on key-value stores, while that for column-oriented stores that support range queries (e.g., scan operation) has not been addressed. In this paper, we also propose two cache strategies of IKNC for column-oriented stores. In our experiments, Apache HBase is running on an application layer, while our IKNC with the proposed cache strategies is implemented on an FPGA-based NIC and its device driver. A significant performance improvement is achieved by the proposed IKNC and pros and cons of the proposed two cache strategies are demonstrated.
Conflict and the Stochastic-Dominance Principle of Decision Making

One of the key principles underlying rational models of decision making is the idea that the decision maker should never choose an action that is stochastically dominated by another action. In the study reported in this article, violations of stochastic dominance frequently occurred when the payoffs produced by two actions were negatively correlated (in conflict), but no violations occurred when the payoffs were positively correlated (no conflict). This finding is contrary to models which assume that choice probability depends on the utility of each action, and the utility for an action depends solely on its own payoffs and probabilities. This article also reports, for the first time ever, the distribution of response times observed in a risky decision task. Both the violations of stochastic dominance and the response time distributions are explained in terms of a dynamic theory of decision making called multiattribute decision field theory.