Miracle-dz/UniFAR-FTUs huggingface dataset:
Manipulating Each MreB of Bdellovibrio bacteriovorus Gives Diverse Morphological and Predatory Phenotypes

ABSTRACT We studied the two mreB genes, encoding actinlike cytoskeletal elements, in the predatory bacterium Bdellovibrio bacteriovorus. This bacterium enters and replicates within other Gram-negative bacteria by attack-phase Bdellovibrio squeezing through prey outer membrane, residing and growing filamentously in the prey periplasm forming an infective “bdelloplast,” and septating after 4 h, once the prey contents are consumed. This lifestyle brings challenges to the Bdellovibrio cytoskeleton. Both mreB genes were essential for viable predatory growth, but C-terminal green fluorescent protein tagging each separately with monomeric teal-fluorescent protein (mTFP) gave two strains with phenotypic changes at different stages in predatory growth and development. MreB1-mTFP cells arrested growth early in bdelloplast formation, despite successful degradation of prey nucleoid. A large population of stalled bdelloplasts formed in predatory cultures and predation proceeded very slowly. A small proportion of bdelloplasts lysed after several days, liberating MreB1-mTFP attack-phase cells of wild-type morphology; this process was aided by subinhibitory concentrations of an MreB-specific inhibitor, A22. MreB2-mTFP, in contrast, was predatory at an almost wild-type rate but yielded attack-phase cells with diverse morphologies, including spherical, elongated, and branched, the first time such phenotypes have been described. Wild-type predatory rates were seen for all but spherical morphotypes, and septation of elongated morphotypes was achieved by the addition of A22.
Insights into Hemoglobin Assembly through in Vivo Mutagenesis of α-Hemoglobin Stabilizing Protein*

Background: The α-hemoglobin-stabilizing protein (AHSP) facilitates hemoglobin assembly. Results: AHSP mutations that enhance binding affinity for α-globin or slow its rate of autooxidation in vitro do not affect normal or stressed erythropoiesis in mice. Conclusion: AHSP exhibits robust molecular chaperone activity in vivo even when its biochemical interactions with reduced α-globin are perturbed. Significance: Our findings support new models for AHSP activities in vivo. α-Hemoglobin stabilizing protein (AHSP) is believed to facilitate adult Hemoglobin A assembly and protect against toxic free α-globin subunits. Recombinant AHSP binds multiple forms of free α-globin to stabilize their structures and inhibit precipitation. However, AHSP also stimulates autooxidation of αO2 subunit and its rapid conversion to a partially unfolded bishistidyl hemichrome structure. To investigate these biochemical properties, we altered the evolutionarily conserved AHSP proline 30 in recombinantly expressed proteins and introduced identical mutations into the endogenous murine Ahsp gene. In vitro, the P30W AHSP variant bound oxygenated α chains with 30-fold increased affinity. Both P30W and P30A mutant proteins also caused decreased rates of αO2 autooxidation as compared with wild-type AHSP. Despite these abnormalities, mice harboring P30A or P30W Ahsp mutations exhibited no detectable defects in erythropoiesis at steady state or during induced stresses. Further biochemical studies revealed that the AHSP P30A and P30W substitutions had minimal effects on AHSP interactions with ferric α subunits. Together, our findings indicate that the ability of AHSP to stabilize nascent α chain folding intermediates prior to hemin reduction and incorporation into adult Hemoglobin A is physiologically more important than AHSP interactions with ferrous αO2 subunits.
Associations of effort-reward imbalance at work and reported symptoms in different groups of male and female public transport workers

This study tests associations between stressful working conditions in terms of effort-reward imbalance and reported physical symptoms in public transport workers. The authors hypothesize that bus and subway drivers suffer from relatively highest level of ill health as compared to repair service workers and administrative personnel, and that a substantial part of this association is due to the imbalance experienced between high effort and low reward. 1,337 employees participated in this study. Measures of effort-reward imbalance were constructed from a large battery of standardized Likert-scaled items, and factor-based symptom scales were computed. In conclusion, stressful working conditions as defined by effort-reward imbalance are associated with high rates of reported symptoms in public transport workers, especially so in professional drivers.
Reconceptualizing the gendered body: learning and constructing masculinities and femininities in school

How children learn to construct and enact masculinities and femininities is clearly an issue for education and one that has been explored in a wide variety of ways. In recent years, however, our conceptions of gender have once again become problematic, particularly given a gradual slippage regarding the sex/gender distinction and the increasing use of ‘gender’ to refer to matters of biology as well as those pertaining to the social. We now need to rethink how we understand what it is to be male and female, masculine and feminine, and whether the sex/gender distinction and related dualisms are useful to our conceptualization of gender. One way to do this is to focus on the construction of gender in the social systems of which children are a part, including the schooling system. In this paper I consider the legacy of Cartesian dualism both for our understanding of sex and gender and for the schooling system, exploring the interconnections between the two. I examine how the Cartesian legacy underpins the disciplinary and curriculum structures of schools and explore the implications for the ways in which we, as researchers and teachers, view and treat children in schools. Finally, I argue that researchers working in gender and education need to take much more account of the specificities of children’s bodies.
Multi-sensor Appearance-Based Place Recognition

This paper describes a multi-sensor appearance-based place recognition system suitable for robotic mapping. Unlike systems that extract features from visual imagery only, here we apply the well known Bag-of-Words approach to features extracted from both visual and range sensors. By applying this technique to both sensor streams simultaneously we can overcome the deficiencies of each individual sensor. We show that LIDAR-based place recognition using a generative model learnt from Variable Dimensional Local Shape Descriptors can be used to perform place recognition regardless of lighting conditions or large changes in orientation, including traversing loops backward. Likewise, we are still able to exploit the feature rich place recognition that visual systems provide. Using a pose verification system we are able to effectively discard false positive loop detections. We present experimental results that highlight the strength of our approach and investigate alternative techniques for combining the results from the individual sensor streams. The multi-sensor approach enables the two sensors to complement each other well in large urban and rural environments under variable lighting conditions.
The Epistemology of Cultural Competence

Cultural competence is a high priority in social work, but it is not conceptualized in a way that can effectively guide practice. The author proposes an organization of cultural competence strategies into epistemologically defined paradigms for multicultural practice. The paradigms discussed are based in postpositivism, constructivism, critical theory, and postmodernism. These paradigms are presented as potential tools for developing a better understanding of what constitutes effective multicultural practice and what contributes to cultural impasses. The author suggests that cultural competence should be defined by the capacity to work across multiple paradigms to find ways to engage with clients.
Is Upregulation of BCL2 a Determinant of Tumor Development Driven by Inactivation of CDH1/E-Cadherin?

Inactivation of CDH1, encoding E-cadherin, promotes cancer initiation and progression. According to a newly proposed molecular mechanism, loss of E-cadherin triggers an upregulation of the anti-apoptotic oncoprotein BCL2. Conversely, reconstitution of E-cadherin counteracts overexpression of BCL2. This reciprocal regulation is thought to be critical for early tumor development. We determined the relevance of this new concept in human infiltrating lobular breast cancer (ILBC), the prime tumor entity associated with CDH1 inactivation. BCL2 expression was examined in human ILBC cell lines (IPH-926, MDA-MB-134, SUM-44) harboring deleterious CDH1 mutations. To test for an intact regulatory axis between E-cadherin and BCL2, wild-type E-cadherin was reconstituted in ILBC cells by ectopic expression. Moreover, BCL2 and E-cadherin were evaluated in primary invasive breast cancers and in synchronous lobular carcinomas in situ (LCIS). MDA-MB-134 and IPH-926 showed little or no BCL2 expression, while SUM-44 ILBC cells were BCL2-positive. Reconstitution of E-cadherin failed to impact on BCL2 expression in all cell lines tested. Primary ILBCs were almost uniformly E-cadherin-negative (97%) and were frequently BCL2-negative (46%). When compared with an appropriate control group, ILBCs showed a trend towards an increased frequency of BCL2-negative cases (P = 0.064). In terminal duct-lobular units affected by LCIS, the E-cadherin-negative neoplastic component showed a similar or a reduced BCL2-immunoreactivity, when compared with the adjacent epithelium. In conclusion, upregulation of BCL2 is not involved in lobular breast carcinogenesis and is unlikely to represent an important determinant of tumor development driven by CDH1 inactivation.
Cubing Algorithms for XML Data

The problem of effectively and efficiently building OLAP data cubes over XML data sources is prominent in next-generation Enterprise Information Systems. The latter is the goal of so-called cubing algorithms, which have attracted a great deal of attention from the Database and Data Warehousing research community. On the basis of this breaking evidence, in this paper we provide a comprehensive survey on cubing algorithms for XML data, along with a critical analysis of these algorithms, and discussion on open issues and future research trends in XML-OLAP research.
Beyond an Assumed Mother–Child Symbiosis in Nutritional Guidelines: The Everyday Reasoning Behind Complementary Feeding Decisions

Researchers question the implications of the way in which “motherhood” is constructed in public health discourse. Current nutritional guidelines for Danish parents of young children are part of this discourse. They are shaped by an assumed symbiotic relationship between the nutritional needs of the child and the interest and focus of the mother. The aim of this qualitative study was to explore mothers' concerns and feeding practices in the context of everyday life. A total of 45 mothers with children either seven months old or 13 months old participated. The results showed that the need to find practical solutions for the whole family in a busy everyday life, to socialise the child into the family and society at large, and to create personal relief from the strain small children put on time and energy all served as socially acceptable reasons for knowingly departing from nutritional recommendations. These findings suggest that information about parental concerns is important both if we are to understand the successes and failures of existing health initiatives on child-feeding and if we are to plan effective interventions in the future. The study suggests that current Danish guidelines for infant feeding do not accord well with the ordinary daily concerns and practices of mothers. Future initiatives should be based on a more dynamic and socially contextualised perspective on motherhood.
Change in Covert Social Movement Networks: The ‘Inner Circle’ of the Provisional Irish Republican Army

In this paper, we contribute to both the growing body of literature on social movement networks and the growing body of literature on change in networks by exploring patterns and mechanisms of change within the network of the ‘inner circle’ of the Provisional Irish Republican Army. Specifically, we focus upon the period between 1969 (when it formed) and 1988 (the last point for which we have been able to gather good data). Our primary aims are substantive. We want to know how this network changed over time. In addition, however, our analysis identifies changes which other analysts might look for in their networks and offers methodological suggestions for those who, like us, find that their networks do not meet the assumptions of mainstream approaches to modelling network dynamics. There is a further dimension to the paper, however. We are studying a covert social movement network. This is a special type of movement network whose organisation and dynamics are predicted to vary from other movement networks. Some have suggested that they are inclined to be relatively static because the need for trust within them is so great and the risk to whatever they hold secret so considerable when new ties are formed that their members tend only to recruit within the pool of their pre-existing ties and actively seek to minimise recruitment and the formation of new ties. One of the aims of our paper was to determine whether and to what extent this is so.
Effect of Tactile Feedback on Movement Speed and Precision During Work-Related Tasks Using a Computer Mouse

Effects of tactile feedback on movement accuracy and speed were studied. Younger and older participants performed three tasks (1, select and drag word; 2, menu navigation; 3, select and drag cell) using commercial software and a mouse with or without tactile feedback. Task time and error number were recorded. Tasks were divided according to presence or absence of tactile feedback, and participants were divided into subgroups (high, average, low) based on Task 1 performance. Overall, older participants took longer (p < .0001) and made more errors (p < .001) than younger participants. There was an effect of feedback by task in younger participants for all six outcomes (p < 0.02). At the task level, with feedback, younger participants reduced performance time (13%) and errors (24%) on Task 1. Low-and average-performance younger participants benefited most from feedback for Task 1. Older low-performance participants also benefited from feedback for Task 1. For Task 3, older participants tended to take more time and make more errors with feedback. Tactile feedback may enhance performance when feedback is event related. Older people may not integrate sensation as well as younger individuals to enhance performance. Potential applications of this research include the development of tactile feedback interfaces to facilitate computer use.
Girls’ Groups as a Component of Anti‐sexist Practice—one primary school's experience

ABSTRACT The gender project I have been involved in was a practical response to theoretical concerns regarding the impact of the hidden curriculum and sex stereotyping in primary schools. The aim of the project was primarily to improve the level of achievement of the girls. Equally important was a desire to broaden curriculum experience into non‐stereotypical areas for both boys and girls. Unlike similar projects, Preston School's single sex groups were monitored throughout their duration. Consequently there are substantially more data on the attitudes of teachers, girls and boys towards gender than are normally available for such projects. This article constitutes an attempt by one of the teachers involved to evaluate the efficacy of Preston School's initiative within a theoretical framework that explores the wider issues of gendered subjectivity, male sexism and female self‐depreciation.
Vergence control learning through real V1 disparity tuning curves

A neural network architecture able to autonomously learn effective disparity-vergence responses and drive the vergence eye movements of a simulated binocular active vision system is proposed. The proposed approach, instead of exploiting purposely designed resources, relies on the direct use of a set of real disparity tuning curves, measured in the primary visual cortex of two macaque monkeys and courteously made available by (Prince et al., 2002), that provides a distributed representation of binocular disparity. The network evolves following a differential Hebbian rule that exploits the overall population activity to measure the state of the system, i.e. the deviation from the desired vergence position, so as its modification as a consequence of the action performed. Accordingly, the signal provides an effective intrinsic reward to develop a stable and accurate vergence behaviour. Emerging from a direct interaction of the sensing system with the environment, the resulting control provides a precise and accurate control for small disparities, as well as a raw control on a broader working range when large disparities are experienced. The developed control converges to a stable state that intrinsically and continuously adapts to the characteristics of the ongoing stimulation. The results proved how actually naturally distributed resources allows for robust and flexible learning capabilities in changeable situations.
STUDY OF SINUSOIDAL AND SPACE VECTOR PULSE WIDTH MODULATION TECHNIQUES FOR A CASCADED THREE-LEVEL INVERTER

This paper compares and evaluates the performance oPulse Width Modulation (SPWM) and Spac e Vector Pulse Width Modulation (SVPWM) techniques for a three-level inv erter by cascading two two-level inverters. In this topology, four power semiconductor switches are used per phase and a tot al of twelve switches are required. The simulation study shows that SVPWM is superior to SPWM in the aspects of better DC-bus ut ilization and offering better spectral performance. Index Terms: space vector modulation, multi-level inverters, si ne-triangle modulation, and cascaded inverter
Uncoupling Scavenger Receptor A-Mediated Phagocytosis of Bacteria from Endotoxic Shock Resistance

ABSTRACT Unresolved infection by gram-negative bacteria can result in the potentially lethal condition known as endotoxic shock, whereby uncontrolled inflammation can lead to multiple organ failure and death of the infected host. Previous results have demonstrated that animals deficient in class A scavenger receptor (SRA), a trafficking receptor for bacteria and bacterium-derived molecules, are more susceptible to endotoxic shock. This has been proposed to be a result of impaired SRA-dependent phagocytic clearance of bacteria resulting in stronger proinflammatory stimuli. In this report, we test the hypothesis that there is an obligate reciprocal relationship between SRA-mediated phagocytosis of bacteria and susceptibility to endotoxic shock. Here, we demonstrate that both SRA-dependent and -independent gram-negative bacterial strains elicit SRA-dependent increased cytokine production in vitro and in vivo and increased susceptibility to endotoxic shock in SRA-deficient mice. This is the first evidence showing that SRA-mediated clearance of LPS is functionally distinct from the role of SRA in bacterial phagocytosis and is a formal demonstration that the SRA-dependent cytokine responses and the resultant endotoxic shock are not coupled to SRA-mediated clearance of bacteria.
Functional and prognostic implications of left ventricular contractile reserve in patients with asymptomatic severe mitral regurgitation

Objective: To evaluate contractile reserve (CR) determined by exercise echocardiography in predicting clinical outcome and left ventricular (LV) function in asymptomatic severe mitral regurgitation (MR). Design: Cohort study. Setting: Regional cardiac centre. Patients and outcome measures: LV volumes and ejection fraction (EF) were measured at rest and after stress in 71 patients with isolated MR. During follow up (mean (SD) 3 (1) years), EF and functional capacity were serially assessed and cardiac events (cardiac death, heart failure, and new atrial fibrillation) were documented. Results: CR was present in 45 patients (CR+) and absent in 26 patients (CR−). Age, resting LV dimensions, EF, and MR severity were similar in both groups. Mitral surgery was performed in 19 of 45 (42%) CR+ patients and 22 of 26 (85%) CR− patients. In patients undergoing surgery, CR was an independent predictor of follow up EF (p = 0.006) and postoperative LV dysfunction (EF < 50%) persisted in five patients, all in the CR− group. Event-free survival was lower in surgically treated patients without CR (p = 0.03). In medically treated patients, follow up EF was preserved in those with intact CR but progressively deteriorated in patients without CR, in whom functional capacity also deteriorated. Conclusions: Evaluation of CR by exercise echocardiography may be useful for risk stratification and may help to optimise the timing of surgery in asymptomatic severe MR.
Community Rates of IgG4 Antibodies to Ascaris Haemoglobin Reflect Changes in Community Egg Loads Following Mass Drug Administration

Background Conventional diagnostic methods for human ascariasis are based on the detection of Ascaris lumbricoides eggs in stool samples. However, studies of ascariasis in pigs have shown that the prevalence and the number of eggs detected in the stool do not correlate well with exposure of the herd to the parasite. On the other hand, an ELISA test measuring antibodies to Ascaris suum haemoglobin (AsHb) has been shown to be useful for estimating transmission intensity on pig farms. In this study, we further characterized the AsHb antigen and screened samples from a population-based study conducted in an area that is endemic for Ascaris lumbricoides in Indonesia to assess changes in AsHb antibody rates and levels in humans following mass drug administration (MDA). Methodology/Principal findings We developed and evaluated an ELISA to detect human IgG4 antibodies to AsHb. We tested 1066 plasma samples collected at different times from 599 subjects who lived in a village in rural Indonesia that was highly endemic for ascariasis. The community received 6 rounds of MDA for lymphatic filariasis with albendazole plus diethylcarbamazine between 2002 and 2007. While the AsHb antibody assay was not sensitive for detecting all individuals with Ascaris eggs in their stools, the percentage of seropositive individuals decreased rapidly following MDA. Reductions in antibody rates reflected decreased mean egg output per person both at the community level and in different age groups. Two years after the last round of MDA the community egg output and antibody prevalence rate were reduced by 81.6% and 78.9% respectively compared to baseline levels. Conclusion/Significance IgG4 antibody levels to AsHb appear to reflect recent exposure to Ascaris. The antibody prevalence rate may be a useful indicator for Ascaris transmission intensity in communities that can be used to assess the impact of control measures on the force of transmission.
Finite case series or infinite single-case studies? Comments on “Case series investigations in cognitive neuropsychology” by Schwartz and Dell (2010)

In this commentary, though acknowledging that a case-series approach in neuropsychology is not always possible, we set out a series of considerations that in our view make this approach generally superior to single-case study. We argue that case-series designs are crucial for theory-testing, assessment of computational models, evaluation of inter-patient variation (including selection criteria, patient homogeneity/heterogeneity, premorbid individual differences, etc.) and to establish solid foundations for the interpretation of behavioural dissociations and associations. We conclude by suggesting that, alongside other neuroscience techniques, case-series cognitive neuropsychology provides a crucial contribution to the future of clinical and cognitive neuroscience.