Miracle-dz/UniFAR-FTUs huggingface dataset:
Repeated intermittent oral amphetamine administration results in locomotor tolerance not sensitization

Background: The phenomenon of locomotor sensitization to injected amphetamine is well-characterised. The increased locomotor activity found acutely is enhanced with repeated intermittent treatment. This effect arises due to hypersensitization of the dopaminergic system and is linked to drug addiction. A clinical population exposed to chronic repeated intermittent amphetamine treatment, such as is found for attention deficit hyperactivity disorder (ADHD), may be expected to be more at risk of addiction following this treatment. However, evidence suggests the opposite may be true. This suggests the route of administration may determine the direction of effects. Aims and methods: We aimed to establish how an oral amphetamine treatment regimen, similar to that used in ADHD, impacts on locomotor activity, specifically whether tolerance or sensitization would arise. Healthy hooded Lister rats were given amphetamine (2 mg/kg, 5 mg/kg and 10 mg/kg) or a vehicle solution once daily for 4 weeks with a 5 day on, 2 day off schedule. Locomotor activity was measured on the first day of treatment to establish the acute effects and on the final day of treatment to examine the chronic effects. Results: As expected, acute doses of amphetamine increased locomotor activity, although this only reached statistical significance for the 5 mg/kg and 10 mg/kg doses. By contrast, after chronic treatment, animals administered these doses showed reduced activity indicating drug tolerance rather than sensitization had occurred. Conclusion: We suggest that the route of administration used in ADHD, which results in more stable and longer duration drug levels in the blood, results in tolerance rather than sensitization and that this effect could explain the reduced likelihood of substance addiction in those treated with psychostimulants for ADHD.
Highly available systems for database applications

As users entrust more and more of their applications to computersystems, the need for systems that are continuously operational (24hours per day) has become even greater. This paper presents asurvey and analysis of representative architectures and techniquesthat have been developed for constructing highly available systemsfor database applications. It then proposes a design of adistributed software subsystem that can serve as a unifiedframework for constructing database application systems that meetvarious requirements for high availability.
Mercury in tunas and blue marlin in the North Pacific Ocean

Models and data from the North Pacific Ocean indicate that mercury concentrations in water and biota are increasing in response to (global or hemispheric) anthropogenic mercury releases. In the present study, we provide an updated record of mercury in yellowfin tuna (Thunnus albacares) caught near Hawaii that confirms an earlier conclusion that mercury concentrations in these fish are increasing at a rate similar to that observed in waters shallower than 1000 m. We also compiled and reanalyzed data from bigeye tuna (Thunnus obesus) and blue marlin (Makaira nigricans) caught near Hawaii in the 1970s and 2000s. Increases in mercury concentrations in bigeye tuna are consistent with the trend found in yellowfin tuna, in both timing and magnitude. The data available for blue marlin do not allow for a fair comparison among years, because mercury concentrations differ between sexes for this species, and sex was identified (or reported) in only 3 of 7 studies. Also, mercury concentrations in blue marlin may be insensitive to modest changes in mercury exposure, because this species appears to have the ability to detoxify mercury. The North Pacific Ocean is a region of both relatively high rates of atmospheric mercury deposition and capture fisheries production. Other data sets that allow temporal comparisons in mercury concentrations, such as pacific cod (Gadus macrocephalus) in Alaskan waters and albacore tuna (Thunnus alalunga) off the US Pacific coast, should be explored further, to aid in understanding human health and ecological risks and to develop additional baseline knowledge for assessing changes in a region expected to respond strongly to reductions in anthropogenic mercury emissions. Environ Toxicol Chem 2017;36:1365–1374. © 2017 SETAC
Who Receives Speech/Language Services by 5 Years of Age in the United States?

PURPOSE We sought to identify factors predictive of or associated with receipt of speech/language services during early childhood. We did so by analyzing data from the Early Childhood Longitudinal Study-Birth Cohort (ECLS-B; Andreassen & Fletcher, 2005), a nationally representative data set maintained by the U.S. Department of Education. We addressed two research questions of particular importance to speech-language pathology practice and policy. First, do early vocabulary delays increase children's likelihood of receiving speech/language services? Second, are minority children systematically less likely to receive these services than otherwise similar White children? METHOD Multivariate logistic regression analyses were performed for a population-based sample of 9,600 children and families participating in the ECLS-B. RESULTS Expressive vocabulary delays by 24 months of age were strongly associated with and predictive of children's receipt of speech/language services at 24, 48, and 60 months of age (adjusted odds ratio range = 4.32-16.60). Black children were less likely to receive speech/language services than otherwise similar White children at 24, 48, and 60 months of age (adjusted odds ratio range = 0.42-0.55). Lower socioeconomic status children and those whose parental primary language was other than English were also less likely to receive services. Being born with very low birth weight also significantly increased children's receipt of services at 24, 48, and 60 months of age. CONCLUSION Expressive vocabulary delays at 24 months of age increase children's risk for later speech/language services. Increased use of culturally and linguistically sensitive practices may help racial/ethnic minority children access needed services.
Cloud-point PEG Glass Surfaces for Imaging of Immobilized Single Molecules by Total-internal-reflection Microscopy

[Abstract] This effective, robust protocol generates glass coverslips coated with biotinfunctionalized polyethylene glycol (PEG), making the glass surface resistant to non-specific absorption of biomolecules, and permitting immobilization of biomolecules for subsequent single-molecule tracking of biochemical reactions. The protocol can be completed in one day, and the coverslips can be stored for at least 1 month. We have confirmed that the PEG surfaces prepared according to the protocol are resistant to non-specific adsorption by a wide range of biomolecules (bacterial, mitochondrial, and human transcription factors, DNA, and RNA) and biological buffers.
A Proposed New Strain of Avian Picornavirus in Broiler Chicken from Brazil

ABSTRACT A new strain of avian picornavirus was identified in fecal samples from broiler chickens in a commercial farm in the municipality of Benevides, Pará, Brazil. Genomic analysis showed it to have a nucleotide identity of 78.4% with the family Picornaviridae, genus Avisivirus, and species Avisivirus A, suggesting that this is a possible new strain within this species.
rOCT2 is a basolateral potential-driven carrier, not an organic cation/proton exchanger.

The driving forces mediating tetraethylammonium (TEA) transport were systematically assessed in Xenopus oocytes and MDCK cells expressing organic cation transporter (OCT) 2 cloned from rat kidney (rOCT2). In rOCT2 cRNA-injected oocytes, uptake of [14C]TEA was saturable, with an estimated Michaelis constant ( K m) of 393 μM, and was specifically inhibited by organic cations. Furthermore, TEA uptake demonstrated two distinct components, one that was potential sensitive and one that was pH sensitive. When membrane potential was intact, TEA uptake was largely unaffected by changes in medium pH; when the oocyte membrane was depolarized (K+ in = out = 102.5 mM, plus valinomycin), decreasing external medium pH significantly reduced TEA uptake. Consistent with the potential sensitivity of uptake, electrophysiological analysis of rOCT2-injected oocytes demonstrated movement of positive charge into the oocyte upon TEA addition. To further evaluate the nature of the pH effect and assess the properties of rOCT2 in a renal epithelium, rOCT2 was introduced into MDCK cells. A stably transfected single cell clone (MDCK-rOCT2) showed mediated, potential-sensitive, pH-sensitive TEA uptake ( K m = 48 μM). TEA efflux from preloaded MDCK-rOCT2 cells was stimulated by externally applied ( trans) tetramethylammonium but was trans-inhibited by H+ (external pH 5.4). The effect of external H+ was to modulate rOCT2-mediated transport. Thus rOCT2 is a potential-driven transporter, not an organic cation/H+exchanger, consistent with a physiological role in the basolateral entry step in renal organic cation secretion.
A Short Tour of Compressive Sensing

Der Dannie-Heineman-Preis 2013 wurde Emmanuel Jean Candès, Stanford/USA, verliehen.Herr Candès hat als einer der Architektendes Compressive Sensing Prinzips die Brücke zwischen Grundlagenforschung und der vielfältigen praktischen Nutzung dieser Theorie hergestellt und hierdurch die Entwicklung der mathematischen Statistik, der angewandten Mathematik und angrenzender Gebiete in jüngster Zeit maßgeblich geprägt.
Assessment of Maximal Isometric Hand Grip Strength in School-aged Children

Abstract Background Hand grip strength (HGS) test is commonly used as an indicator of overall muscle strength in medical and sport practices. Recently, several studies have proposed that the measurement of the maximal HGS depends on dynamometer’s handle position. The aim of the present study was to identify the optimal handle position to obtain maximal HGS using the hand grip dynamometer (HGD) for school-aged children. Methods HGS was assessed with the Jamar digital HGD. Each participant performed three maximum contractions of each hand on three handle positions progressing from first to third position. Results A total of 135 healthy children aged 5–9 years participated in the study. Participants obtained significantly higher results using position 2 than using positions 1 or 3. The maximal mean (± SD) HGS achieved was 9.9 (± 3.1) kg with position 1, 10.4 (± 3.1) kg with position 2, and 9.0 (± 3.2) kg with position 3. Handle position 2 was the most comfortable position for 73% of participants. Conclusions Our results provide useful methodological information indicating that the second handle position of the Jamar digital HGD is optimal to measure maximal HGS in non-athletic healthy pediatric participants aged 5–9 years.
Implementing of a problem-based learning strategy in a Saudi medical school: requisites and challenges

Due to the current changes in healthcare realities and the continuing expansion of knowledge, there is an increasing demand for cognitive and problem-solving abilities to recognize patient and society’s needs.1,2 The health needs might be accomplished by making changes in traditional learning strategies to a more student-centered approach and utilizing flexible educational strategies as well as problem-based learning (PBL). 2 The educational goals of PBL allow students to integrate basic science knowledge into the solution of clinical problems and ultimately serve better the future patient.3,4 In this article, we explore our experience in implementation of a hybrid PBL curriculum at the University of Bisha, College of Medicine (UBCOM), Saudi Arabia. Initially, we will briefly summarize the existing state of medical education in Saudi medical schools, followed by a description of PBL curriculum structure and its implementation challenges faced at UBCOM.
Automation of Processor Verification Using Recurrent Neural Networks

When considering simulation-based verification of processors, the current trend is to generate stimuli using pseudorandom generators (PRGs), apply them to the processor inputs and monitor the achieved coverage of its functionality in order to determine verification completeness. Stimuli can have different forms, for example, they can be represented by bit vectors applied to the input ports of the processor or by programs that are loaded directly into the program memory. In this paper, we propose a new technique dynamically altering constraints for PRG via recurrent neural network, which receives a coverage feedback from the simulation of design under verification. For the demonstration purposes we used processors provided by Codasip as their coverage state space is reasonably big and differs for various kinds of processors. Nevertheless, techniques presented in this paper are widely applicable. The results of experiments show that not only the coverage closure is achieved much sooner, but we are able to isolate a small set of stimuli with high coverage that can be used for running regression tests.
Melt spinning of high-strength fiber from low-molecular-weight polypropylene

Polypropylene (PP) with high melt flow index (MFI) or low molecular weight, although desired in melt spinning for enhanced productivity, is difficult to be spun into high-strength fiber using the standard process where extensive jet stretching is applied. In this work, a processing route involving minimal jet stretch has been explored. A two-stage hot drawing procedure in the solid state was found to be suitable for producing high-strength fiber from low-molecular-weight PP with an ultrahigh MFI of 115 g/10 min. Fibers produced achieve a maximum tensile strength and Young's modulus of approximately 600 MPa and 12 GPa, respectively. The melt temperature of the fiber reached 170.8°C, approximately 5°C higher than that of the original resin. Wide-angle X-ray diffraction (WAXD) study shows that the stable α-monoclinic crystalline structure is developed during the drawing process. A well-oriented crystalline structure along the fiber axis is generated, having a crystalline orientation factor as high as 0.84. POLYM. ENG. SCI., 2015. © 2015 Society of Plastics Engineers
Lolisa: Formal Syntax and Semantics for a Subset of the Solidity Programming Language

This article presents the formal syntax and semantics for a large subset of the Solidity programming language developed for the Etheruem blockchain platform based on our resent work about developing a general, extensible, and reusable formal memory (GERM) framework and an extension of Curry-Howard isomorphism, denoted as execution-verification isomorphism (EVI). This subset is denoted as Lolisa, which, to our knowledge, is the first mechanized and validated formal syntax and semantics developed for Solidity. The formal syntax of Lolisa adopts a stronger static type system than Solidity for enhanced type safety. In addition, Lolisa not only includes nearly all the syntax components of Solidity, such as mapping, modifier, contract, and address types, but it also contains general-purpose programming language features, such as multiple return values, pointer arithmetic, struct, and field access. Therefore, the inherent compatibility of Lolisa allows Solidity programs to be directly translated into Lolisa with a line-by-line correspondence without rebuilding or abstracting, and, in addition, the inherent generality of Lolisa allows it to be extended to express other programming languages as well. To this end, we also present a preliminary scheme for extending Lolisa to other languages systematically.
Physical Principles, Use of High b-Values And Clinical Applications of Diffusion-Weighted Imaging of Ischemic Stroke

Diffusion weighted imaging has been a highly sensitive technique and a great success in neuroimaging for the early detection of water diffusion in cerebral ischemia. A diffusion weighted image provides the contrast, based on microscopic molecular motion and shows the changes in MRI signal intensity that is not possible with conventional T1- weighted and T2-weighted images. The addition of two strong diffusion gradient pulses to the normal MRI pulse sequences, generate the images being water-motion sensitized in the direction of applied gradients. This process helps to determine the diffusion weighting of the images, characterized by gradient factor b. In images that are obtained with Fast Spin Echo Imaging (FSEI), Segmented Echo Planar Imaging (SEPI), Single Shot Echo Planar Imaging (SSEPI), like quick imaging modalities, bulk motion related artifact can be minimized. Consequently imaging time will be shortened and image quality would be better and much more enhanced. Conclusively, DWI is a non-invasive technique that provides exceptional information and the utility for monitoring and predicting response to treatment.
Short-term rehospitalisation or death and determinants after admission for acute heart failure in a cohort of African patients in Port Harcourt, southern Nigeria

Summary Background Heart failure (HF) is a major health burden globally and contributes significantly to morbidity and mortality related to cardiovascular disease. The aim of this study was to determine the outcome, and factors determining these outcomes in patients admitted for acute HF and followed up for six months. Methods This was a hospital-based, prospective study. Subjects included consecutive patients with a confirmed diagnosis of acute HF admitted to the medical wards of the University of Port Harcourt Teaching Hospital (UPTH) in Nigeria over one year. All had a full physical examination and relevant investigations, including echocardiography. Subjects were followed up for six months and reassessed for outcome/ endpoint, which was rehospitalisation or death. Factors that predicted these outcomes were also determined. Results There were 160 subjects, 84 females and 76 males, age range 20 to 87 years, mean age 52.49 ± 13.89 years. Sixteen subjects (10.0%) were lost to follow up, 66 (41.3%) showed clinical improvement, 57 (35.6%) were rehospitalised, while 21 (13.1%) died. Determinants of rehospitalisation were New York Heart Association (NYHA) class, heart failure type, haemoglobin level at presentation and estimated glomerular filtration rate (eGFR). Determinants of mortality were NYHA class and haemoglobin level at presentation. Conclusion Heart failure rehospitalisation and mortality rates of 35.6 and 13.1%, respectively, were high compared to developed countries.
Effective heart disease prediction system using data mining techniques

The health care industries collect huge amounts of data that contain some hidden information, which is useful for making effective decisions. For providing appropriate results and making effective decisions on data, some advanced data mining techniques are used. In this study, an effective heart disease prediction system (EHDPS) is developed using neural network for predicting the risk level of heart disease. The system uses 15 medical parameters such as age, sex, blood pressure, cholesterol, and obesity for prediction. The EHDPS predicts the likelihood of patients getting heart disease. It enables significant knowledge, eg, relationships between medical factors related to heart disease and patterns, to be established. We have employed the multilayer perceptron neural network with backpropagation as the training algorithm. The obtained results have illustrated that the designed diagnostic system can effectively predict the risk level of heart diseases.
Applying single-molecule localisation microscopy to achieve virtual optical sectioning and study T-cell activation

Single-molecule localisation microscopy (SMLM) allows imaging of fluorescently-tagged proteins in live cells with a precision well below that of the diffraction limit. As a single-molecule technique, it has also introduced a new quantitative approach to fluorescence microscopy. In the Part A of this thesis, the design and building of three SMLM instruments, the implementation of a custom-developed image analysis package and the characterisation of the photo-physical properties of the photo-activable fluorescent protein used in this thesis (mEos), are discussed. Then, a new post-processing method for SMLM analysis is characterised: axial optical sectioning of SMLM images is demonstrated by thresholding fitted localisations using their fitted width and amplitude to reject fluorophores that emit from above or below a virtual ‘light-sheet’, a thin volume centred on the focal plane of the microscope. This method provides qualitative and quantitative improvements
Effect of drug-related cues on response inhibition through abstinence: A pilot study in male heroin abstainers

ABSTRACT Background: Chronic heroin use can cause a deficit of inhibitory function, leading to a loss of control over drug use. Exposure to drug-related cues is considered as one of the contributing factors. However, it is unclear whether there are dynamic changes on the effect of drug-related cues on response inhibition following prolonged abstinence. Objective: The present study investigated the effect of drug-related cues on response inhibition in heroin abstainers at different abstinent phases. Methods: 26 shorter-term (2–6 months) and 26 longer-term (19–24 months) male heroin abstainers performed on a modified two-choice Oddball task, which included two conditions: in the cued condition, neutral pictures served as the background of standard stimuli (yellow frame) and heroin-related pictures served as the background of deviant stimuli (blue frame), reversed in the controlled conditions. Results: Compared to longer-term abstainers, mean reaction time (RT) for drug deviants in shorter-term abstainers was significantly longer. Shorter-term abstainers also showed markedly slower response to neutral deviants relative to drug deviants, but this tendency was not observed in longer-term abstainers. Nevertheless, both groups had similar RT for standard stimuli regardless of their paired background pictures. Conclusion: Effect of drug-related cues on response inhibition remains at the early stage of abstinence; however, this effect may be reduced following a longer period of drug abstinence. Our findings highlight the importance of assessing and improving the ability of inhibiting drug-related cue reactivity during treatment.
Maternal nutrient restriction during pregnancy and lactation leads to impaired right ventricular function in young adult baboons

Maternal nutrient restriction induces intrauterine growth restriction (IUGR) and leads to heightened cardiovascular risks later in life. We report right ventricular (RV) filling and ejection abnormalities in IUGR young adult baboons using cardiac magnetic resonance imaging. Both functional and morphological indicators of poor RV function were seen, many of which were similar to effects of ageing, but also with a few key differences. We observed more pronounced RV changes compared to our previous report of the left ventricle, suggesting there is likely to be a component of isolated RV abnormality in addition to expected haemodynamic sequelae from left ventricular dysfunction. In particular, our findings raise the suspicion of pulmonary hypertension after IUGR. This study establishes that IUGR also leads to impairment of the right ventricle in addition to the left ventricle classically studied.