Miracle-dz/UniFAR-FTUs huggingface dataset:
Probing transient copper chaperone-Wilson disease protein interactions at the single-molecule level with nanovesicle trapping.

Transient metallochaperone−target protein interactions are essential for intracellular metal trafficking but challenging to study at both the ensemble and the single-molecule level. Here we report using nanovesicle trapping to enable single-molecule fluorescence resonance energy transfer (smFRET) studies of transient interactions between the copper chaperone Hah1 and the fourth metal-binding domain of its target protein, the Wilson disease protein (WDP). We were able to monitor their interactions in real time one event at a time, capture distinct protein interaction intermediates, resolve intermediate interconversion dynamics, and quantify both the interaction kinetics and thermodynamics in the absence of copper. The study exemplifies the ability of nanovesicle trapping in combination with smFRET for studying weak protein interactions and provides insight into how Hah1 and WDP may collaborate to mediate copper transfer inside cells.
PERFIL EPIDEMIOLÓGICO DE OCORRÊNCIAS NO TRÂNSITO NO BRASIL - REVISÃO INTEGRATIVA

Revisao integrativa da literatura, realizada por publicacoes cientificas publicadas no ano de 2009 a 2013, que teve como objetivo analisar o perfil epidemiologico das vitimas acometidas por acidentes de trânsito nas cidades do Brasil. A busca na literatura ocorreu nos meses de outubro e novembro de 2013. As bases de dados consultadas foram Scientific Electronic Library On-line (SciELO) e Literatura Latino-Americana e de Caribe em Ciencias da Saude (LILACS). Os descritores utilizados foram selecionados de acordo com as terminologias consultadas nos Descritores em Ciencias da Saude (DECS-BIREME), foram eles: “acidentes de trânsito” e “perfil epidemiologico”. A busca considerou as publicacoes dos ultimos cinco anos (2009 a 2013), onde resultou no total de 59 estudos. Foram aplicados os criterios de inclusao e exclusao, onde resultaram apenas 9 estudos completos. A partir da analise dos artigos selecionados foi possivel verificar o crescente aumento do numero de acidentes de trânsito no Brasil. Adultos jovens, do genero masculino, foram os que mais se envolveram em acidentes de trânsito. O veiculo mais citado nos estudos foram as motocicletas, sendo as causas mais relacionadas: uso do alcool, alta velocidade, inexperiencia por parte de alguns, e o nao uso de equipamentos de protecao individual (EPI) necessarios para a utilizacao deste veiculo, originando uma contribuicao direta para o aumento da morbimortalidade da populacao, sendo indispensavel a atuacao de autoridades e profissionais de saude para que haja um envolvimento da populacao nas acoes preventivas.
Disturbance in the self-image at adolescence.

The purpose of this cross-sectional study was to investigate whether adolescence is a period of disturbance for the child's self-image and, if so, at what age of adolescence and under what social conditions the disturbance is greatest. Several dimensions of the self-image were measured among 1,917 urban school children in grades three through twelve. Compared to children in the eight to eleven age group, the early adolescents, particularly those between twelve and thirteen, were shown to exhibit heightened self-consciousness, greater instability of the self-image, slightly lower self-esteem, and a less favorable view of the opinions held of them by significant others. Evidence is presented suggesting that the child's environment may have a stronger effect than his age in producing such changes. Children who had entered junior high school appeared more disturbed along these lines than their age-peers still in elementary school.
Scalable Rekeying Algorithm in IEEE 802.16e

IEEE 802.16e standard provides wide coverage and high bandwidth for subscribers in a metropolitan area network. It introduces Multicast and Broadcast Rekeying Algorithm (MBRA) which is a multicasting scheme to communicate with many users concurrently. Although ELAPSE (for Efficient sub-Linear rekeying Algorithm with Perfect SEcrecy) improves on the deficiencies of MBRA, the algorithm poorly responds to scalability issue. This paper proposes a Scalable Rekeying Algorithm (SRA) based on a complete binary tree structure. SRA is introduced with linear linked list structure in order to make the system more scalable. Evaluation analysis shows that SRA manages to improve the scalability issue in MBRA for Mobile WiMAX.
Potassium channel inhibition reduces cell proliferation in the GH3 pituitary cell line

Potassium (K+) conductances are known to be involved in cell proliferation of a number of nonexcitable cell types. The nature of the mechanism by which K+ channel inhibition reduces cell proliferation has remained elusive despite intensive search. We investigated whether such a phenomenon could be demonstrated in excitable cells, using the GH3 pituitary cell line as a cell model. Our aims were: (1) to study the effect of K+ channel inhibition on the proliferation of GH3 cells; and (2) to investigate the putative intracellular signals involved in this inhibition. Tetraethylammonium chloride (TEA), a blocker of the calcium (Ca2+)‐dependent K+ conductances of GH3, was found to reversibly inhibit cell proliferation, as measured by 3H‐thymidine incorporation. Cell cycle block specifically occurred at the G1/S phase of the cell cycle. This inhibition of proliferation was observed for 1–4 mM TEA, which suppressed most of the Ca2+‐activated K+ current and part of the inward rectifying K+ current, as shown by electrophysiological experiments. Increasing extracellular K+ concentrations with KCl also inhibited cell proliferation in a dose‐dependent manner. Both TEA and KCl depolarized the cells and increased intracellular Ca2+ levels ([Ca2+]i), showing that, in this type of excitable cell, inhibition of cell proliferation can be associated with elevated Ca2+ levels. Ca2+ and membrane resting potential (MRP) were considered as possible messengers of this inhibition. Our results suggest that cell cycle arrest of GH3 cells by K+ channel block probably involves an additional pathway, distinct from those of Ca2+ and MRP. J. Cell. Physiol. 177:402–410, 1998. © 1998 Wiley‐Liss, Inc.
Design for Delay Measurement Aimed at Detecting Small Delay Defects on Global Routing Resources in FPGA

SUMMARY Small delay defects can cause serious issues such as very short lifetime in the recent VLSI devices. Delay measurement is useful to detect small delay defects in manufacturing testing. This paper presents a design for delay measurement to detect small delay defects on global routing resources, such as double, hex and long lines, in a Xilinx Virtex 4 based FPGA. This paper also shows a measurement method using the proposed design. The proposed measurement method is based on an existing one for SoC using delay value measurement circuit (DVMC). The proposed measurement modiﬁes the construction of conﬁgurable logic blocks (CLBs) and utilizes an on-chip DVMC newly added. The number of conﬁgurations required by the proposed measurement is 60, which is comparable to that required by stuck-at fault testing for global routing resources in FPGAs. The area overhead is low for general FPGAs, in which the area of routing resources is much larger than that of the other elements such as CLBs. The area of every modiﬁed CLB is 7% larger than an original CLB, and the area of the on-chip DVMC is 22% as large as that of an original CLB. For recent FPGAs, we can estimate that the area overhead is approximately 2% or less of the FPGAs.
Proposal of a new ultra low leakage 10T sub threshold SRAM bitcell

The tendency for low energy consumption in systems-on-chip results in a need for memories operating in the near- and sub-threshold regions. This paper gives a comparative study of Static Random Access Memory (SRAM) bitcells working under Ultra-Low Voltage in 32nm CMOS. A new 10T SRAM bitcell is then proposed and features low leakage current. It is capable of operation under ULV (~300mV) and allows bit-interleaving technique that is critical to cope with multiple bit soft-errors for reducing dynamic and static power consumption compared to state-of-the-art bitcells.
Systematic Applications of Metabolomics in Metabolic Engineering

The goals of metabolic engineering are well-served by the biological information provided by metabolomics: information on how the cell is currently using its biochemical resources is perhaps one of the best ways to inform strategies to engineer a cell to produce a target compound. Using the analysis of extracellular or intracellular levels of the target compound (or a few closely related molecules) to drive metabolic engineering is quite common. However, there is surprisingly little systematic use of metabolomics datasets, which simultaneously measure hundreds of metabolites rather than just a few, for that same purpose. Here, we review the most common systematic approaches to integrating metabolite data with metabolic engineering, with emphasis on existing efforts to use whole-metabolome datasets. We then review some of the most common approaches for computational modeling of cell-wide metabolism, including constraint-based models, and discuss current computational approaches that explicitly use metabolomics data. We conclude with discussion of the broader potential of computational approaches that systematically use metabolomics data to drive metabolic engineering.
Latent infections in spinal ganglia with thymidine kinase-deficient herpes simplex virus

A herpes simplex virus type 1 variant [C239(TK-)] harboring a deletion in the thymidine kinase (TK) gene was assessed for capacity to establish latent infections. Outbred Swiss Webster mice were inoculated on both hind footpads, and numbers of neurons expressing latency-associated transcript and amounts of viral DNA in latently infected lumbosacral spinal ganglia were scored. C239(TK-) established levels of latent infection that were only slightly lower than those found for either a TK rescued variant of this agent or the parent wild-type KOS. However, in contrast to the TK+ viruses, C239(TK-) could not be reactivated when spinal ganglia were cultured in vitro. The results presented show that expression of the viral TK gene plays no major role in establishment of the latent state but that it functions during reactivation of latent virus from explanted ganglia maintained in vitro.
Reversal of the sleep/wake cycle disorder of sleeping sickness after trypanosomicide treatment

To determine whether the circadian disruption of the sleep/wake cycle observed in sleeping sickness, human African trypanosomiasis (HAT), can be reversed after trypanosomicide treatment, 10 Congolese patients infected by Trypanosoma brucei gambiense underwent 24‐h polysomnographic recordings before treatment with melarsoprol and after each of three weekly treatment sessions. Polysomnography consisted of a continuous recording of the electroencephalogram, electromyogram and electro‐oculogram on a Minidix Alvar polygraph. Sleep traces were analysed in 20‐sec epochs for wakefulness, REM sleep, and NREM sleep [stages 1, 2, 3, 4; stages 3 and 4 representing slow‐wave sleep (SWS)]. As previously described (Buguet et al. 1993), the 24‐h distribution of the sleep/wake cycle was disturbed proportionally to the severity of the illness. The overall amounts of each sleep/wake stage did not change after treatment. However, the patterns of occurrence of sleep episodes, REM sleep and SWS phases were determinant in the evaluation of treatment efficacy. The trypanosomicide action of melarsoprol led to a reduction in the number of sleep episodes, except in one patient whose health condition worsened during the third treatment session: sleep onset REM sleep phases (SOREMPs) decreased and the number of SWS episodes during a sleep episode increased. We conclude that in HAT, the reversibility of the sleep/wake cycle alteration and that of sleep structure constitute the basis for an evaluation of the healing process.
The Collapse of the Assyrian Empire and the Continuity of Ceramic Culture: The Case of the Red House at Tall Sheikh Hamad

The complete ground plan (5400 m 2 ) of the so-called Red House has been excavated at Tall Sheikh Hamad/Dur-Katlimmu on the Habur River in Northern Mesopotamia. Cuneiform tablets can date the period in which the Red House was in use to the reign of the Neo-Babylonian king Nebuchadnezzar II after the fall of the Neo-Assyrian Empire. Large quantities of pottery were found on the floors covered by the destruction debris. Pottery from go rooms has provided insight into a wide range of forms and their functions, which occurred simultaneously. The analysis of the stratigraphic sequence covering a time span from the late Neo-Assyrian period (seventh century BC) to the turn of the sixth to the fifth century BC clearly demonstrates that there is continuity in both wares and forms. This result calls for a re-evaluation of Iron Age chronology based on pottery periodisation, especially the differentiation of Neo-Assyrian and 'Post-Assyrian' pottery.
Effect of LASIK and contact lens corneal refractive therapy on higher order aberrations and contrast sensitivity function.

PURPOSE Corneal and total higher order eye aberrations and their effects on visual function were compared in two groups subjected to different emmetropization techniques-refractive surgery (LASIK) and corneal refractive therapy (CRT). METHODS Twenty eyes of 11 patients and 22 eyes of 13 patients were examined in the CRT and LASIK groups, respectively. Mesopic contrast sensitivity function (CSF) was studied monocularly and binocularly in the two groups of patients undergoing CRT and LASIK who had approximately the same mean refractive error. Corneal asphericity and corneal and total aberrations were calculated, and the differences were analyzed between groups. Visual acuity, corneal and total ocular aberrations, corneal asphericity, and mesopic CSF were determined before and 3 months after treatment. RESULTS In both groups, corneal and total higher order aberrations increased after treatment, and the change in some Zernike coefficients was significant. Mesopic CSF diminished after treatment under monocular as well as binocular conditions, with the greatest decline being found in the CRT group under binocular conditions. Corneal asphericity also increased significantly in both groups, which was more accentuated in the CRT group. CONCLUSIONS Both emmetropization techniques increased higher order ocular aberrations, implying a decline in the mesopic CSF. The increase in aberrations and the consequent deterioration in visual function was more marked in the CRT group.
Coupled Line Filters Using Via-Less End-Connected Broadside Coupled Asymmetric Coplanar Striplines

End-connected broadside coupled asymmetric coplanar stripline (ACPS) is proposed and characterized through conformal mapping for application in via-less coupled line filters. The exact similar derived parameters to the conventional ACPS indicate feasibility of using end-connected broadside coupled ACPS in ACPS applications. The end connection can be implemented using copper foil to eliminate the need for vias. Three coupled line filters for different bandwidths of 50%, 75%, and 100% with 1.7–1.85-dB insertion loss are fabricated and measured to demonstrate feasibility. To further improve the selectivity of the filter, a transmission zero is introduced below the passband by adding an inductor.
Ninja: Towards Transparent Tracing and Debugging on ARM

Existing malware analysis platforms leave detectable ﬁn-gerprints like uncommon string properties in QEMU, signatures in Android Java virtual machine, and artifacts in Linux kernel proﬁles. Since these ﬁngerprints provide the malware a chance to split its behavior depending on whether the analysis system is present or not, existing analysis systems are not sufﬁcient to analyze the sophisticated malware. In this paper, we pro-pose N INJA , a transparent malware analysis framework on ARM platform with low artifacts. N INJA leverages a hardware-assisted isolated execution environment Trust-Zone to transparently trace and debug a target application with the help of Performance Monitor Unit and Embedded Trace Macrocell. N INJA does not modify system software and is OS-agnostic on ARM platform. We implement a prototype of N INJA (i.e., tracing and debugging subsystems), and the experiment results show that N INJA is efﬁcient and transparent for malware analysis.
Acetonitriletrichloridobis(cyclohexyldiphenylphosphane)rhodium(III) acetonitrile disolvate

In the title compound, [RhCl3(CH3CN)(C18H21P)2]·2CH3CN, the complex molecule lies on a twofold rotation axis that passes through the RhIII atom, one Cl atom, and the C and N atoms of the coordinated acetonitrile molecule. The RhIII atom is coordinated by two P atoms in trans positions, three Cl atoms and an acetonitrile molecule in a distorted octahedral geometry. Intramolecular C—H⋯Cl interactions are observed. The uncoordinated acetonitrile molecule is disordered over two sites with occupancies of 0.588 (4) and 0.412 (4).
Treatment of complex aortic aneurysms with fenestrated endografts and chimney stent repair: Systematic review and meta-analysis

Objective We reviewed data pertaining to fenestrated endograft technique and chimney stent repair of complex aortic aneurysm for comparative analysis of the outcomes. Methods A comprehensive search of relevant databases was conducted to identify articles in English, related to the treatment of complex aortic aneurysm with fenestrated endovascular aneurysm repair and chimney stent repair, published until January 2015. Results A total of 42 relevant studies and 2264 patients with aortic aneurysm undergoing fenestrated endovascular aneurysm repair and chimney stent repair were included in our review. A total of 4413 vessels were involved in these processes. The cumulative 30-day mortality was 2.4% and 3.2% (p = 0.459). The follow-up aneurysm-related mortality was 1.4% and 3.2% (p = 0.018), and target organ dysfunction was 5.0% and 4.0% in fenestrated endovascular aneurysm repair and chimney stent repair, respectively (p = 0.27). A total of 156 vessels showed restenosis or occlusion after primary intervention (3.6% and 3.4% in fenestrated endovascular aneurysm repair and chimney stent repair, respectively, p = 0.792). The cumulative type I endoleak was 2.0% (38/1884) after fenestrated endovascular aneurysm repair compared with 3.4% (13/380) after chimney stent repair (p = 0.092), and the type II endoleak was 5.4% (102/1884) and 5.3% (20/380), respectively (p = 0.905). Approximately, 1.1% and 1.6% increase in aneurysm was observed following fenestrated endovascular aneurysm repair and chimney stent repair, respectively (p = 0.437). The re-intervention frequency was 205 and 19 cases after fenestrated endovascular aneurysm repair and chimney stent repair, respectively (11.7%, 5.6%, p = 0.001). Conclusions Fenestrated endovascular aneurysm repair and chimney stent repair are safe and effective in treating patients with complex aortic aneurysm. A higher aneurysm-related mortality was observed in chimney stent repair while fenestrated endovascular aneurysm repair was associated with a higher re-intervention rate.
Does Thyroid Volume and Nodule Formation Increase in Patients with Euthyroid Metabolic Syndrome

Objective: Metabolic syndrome and its related components are associated with functional and morphological alterations of the thyroid gland. The aim of our study is to examine thyroid volume and nodule prevalence in a case– control study of patients with metabolic syndrome. Design: 114 newly diagnosed euthyroid patients with Met S were randomly matched for age and gender with 123 subjects without Met S. Serum TSH, fT3, fT4, insulin resistance which was estimated by the HOMA-IR, as well as other Met S parameters were evaluated in both group. Thyroid ultrasonography was performed in all subjects. All subjects with thyroid nodules >1 cm were offered to undergo thyroid fine needle aspiration biopsy. Results: The study include a total of 237 patients. Among the 114 patients with Met S in the study, 18 were male and 96 were female. There were 123 control subjects, of whom 18 were male and 105 were female. Serum concentrations of FT3, FT4 and TSH were not significantly different between Met S and control group. Mean thyroid volume was significantly higher in Met S patients than in controls (p=0,008). Percentage of patients with thyroid nodules were similar. Thyroid volume was significantly negative correlated with TSH (r=0, 14, p=0, 02), and positive correlated with HOMA-IR (r=0,035, p=0,001). Four thyroid cancer cases were diagnosed in 47 Met S patients (8,5 %) with thyroid nodules undergoing FNAB and two thyroid cancer cases were diagnosed in control subjects (n=42, 4,76 % ) There was no statistically significant difference between two groups (p>0, 05). Conclusions: According to our results, thyroid volume was found to be significantly higher in the metabolic syndrome group than the control group. The incidence of thyroid nodules and thyroid cancer was similar in both group (p>0,05).
Fluoroscopically guided caudal epidural steroid injections in degenerative lumbar spine stenosis.

BACKGROUND Caudal epidural steroid injections are commonly utilized to help reduce radicular pain in lumbar spinal stenosis. There have been studies done to evaluate the effectiveness of this procedure non-fluoroscopically guided. Search revealed no prospective studies evaluating the effectiveness of fluoroscopically guided caudal epidural injections on patients with bilateral radicular pain from degenerative lumbar spinal stenosis. OBJECTIVE To evaluate the therapeutic benefit of fluoroscopically guided caudal epidural steroid injections in the treatment of bilateral radicular pain from symptomatic Degenerative Lumbar Spinal Stenosis (DLSS). DESIGN This prospective cohort study was performed on 34 patients with bilateral radicular pain from lumbar spinal stenosis who received fluoroscopically guided caudal epidural injections at a multidisciplinary spine center as they did not improve with conservative care. The patients' degenerative lumbar spinal stenosis was confirmed by magnetic resonance imaging and classified as mild, moderate, or severe. The patients were evaluated by an independent observer and completed questionnaires, prior to initial injection, at 6 weeks, 6 months and 12 months after the injections. OUTCOME MEASURES Visual analog scale, patient satisfaction scale, standing/walking tolerance scale and Oswestry low back pain disability questionnaire. RESULTS A total of 34 patients met our inclusion criteria and were followed at 6 weeks, 6 months, and 12 months. Sixty-five percent of patients at 6 weeks, 62% at 6 months, and 54% at 12 months had a successful outcome, reporting at least a >50% reduction between pre-injection and post injection visual analog pain scores. Fifty nine percent of patients had an improved walking tolerance at 6 weeks (p <0.0001), 56% at 6 months (p <0.0001), and 51% at 12 months (p=0.0005). Fifty percent of patients had an improved standing tolerance at 6 weeks (p= 0.0002), 54% at 6 months (p < 0.0001), and 51% at 12 months (p=0.0005). The patient satisfaction scale revealed 64% of patients felt completely or somewhat better at 6 weeks, 59% at 6 months and 52% at 12 months. Owestry low back pain disability questionnaire scores showed statistically significant improvement from initial scores to 6 weeks (p < 0.0001), initial to 6 months (p= 0.0095), and initial to 12 months (p=0.00015). The outcome was statistically significant even in severe stenotic patients when comparing initial mean scores to 12 month mean scores in standing tolerance (p =0.2956), walking tolerance (p=0.0250), and VAS (p= 0.0199). CONCLUSION Fluoroscopically guided caudal epidural steroid injections may help reduce bilateral radicular pain and improve standing and walking tolerance in patients with DLSS.