Miracle-dz/UniFAR-FTUs huggingface dataset:
Nurses' Educational Needs for Pain Management of Post-Cardiac Surgery Patients: A Qualitative Study

Background and Research Objective:Inadequate knowledge among health care providers is a key barrier to good pain management, and nurses have a major role to provide education to patients. The purpose of this study was to identify nurses' learning needs to prepare patients for managing pain before and after discharge home from cardiac surgery. The overall aim is to develop a pain education intervention for nurses working with cardiac surgical patients. Subject and Methods:This was a focus groups study. Participants (N = 22) were asked about their perceptions of patients' education needs for pain management after cardiac surgery and approaches to help nurses meet these needs. The Pain Beliefs Scale was used to capture nurses' own misbeliefs about pain that would need clarification in a successful pain education intervention. Results:Nurses identified pain management challenges in the hospital, particularly related to patients' age, patient concerns about the use of opioids, the need to use multiple management strategies, and preparing patients to manage pain at home. Pain Beliefs Scale scores were low related to opioid dosing and adverse effects. Participants identified their most helpful educational approaches being brief in-services, hands-on learning, lunch-and-learn sessions, and designated education days. Conclusion:Participants identified the most common pain knowledge gaps for patients before and after discharge after cardiac surgery. These data will be used to develop an education intervention for nurses to help their cardiac surgery patients with more effective pain management strategies before and after discharge home.
Turn-taking: a case study of early gesture and word use in answering WHERE and WHICH questions

When young children answer questions, they do so more slowly than adults and appear to have difficulty finding the appropriate words. Because children leave gaps before they respond, it is possible that they could answer faster with gestures than with words. In this study, we compare gestural and verbal responses from one child between the ages of 1;4 and 3;5, to adult Where and Which questions, which can be answered with gestures and/or words. After extracting all adult Where and Which questions and child answers from longitudinal videotaped sessions, we examined the timing from the end of each question to the start of the response, and compared the timing for gestures and words. Child responses could take the form of a gesture or word(s); the latter could be words repeated from the adult question or new words retrieved by the child. Or responses could be complex: a gesture + word repeat, gesture + new word, or word repeat + new word. Gestures were the fastest overall, followed successively by word-repeats, then new-word responses. This ordering, with gestures ahead of words, suggests that the child knows what to answer but needs more time to retrieve any relevant words. In short, word retrieval and articulation appear to be bottlenecks in the timing of responses: both add to the planning required in answering a question.
De l'annotation automatique des génomes à l'annotation experte pour la génomique environnementale

A l'epoque du demarrage des travaux presentes dans ce document (2003) l’annotation d’un genome etait une tâche longue et fastidieuse. Avec l’apparition des nouvelles technologies de sequencage, de nombreux outils ont ete developpes pour faciliter et accelerer ce processus. Pour les meilleurs, l’annotation d’un genome automatique peut prendre moins de 3 minutes, reportant l’activite chronophage sur l’annotation manuelle. Ainsi, de nombreux genomes sont deposes dans les banques de sequences tels quels sans annotation manuelle experte. Il est donc rapidement apparu necessaire de fournir aux annotateurs la possibilite d’acceder a des bases de donnees consolidees et specifiques de leur domaine d’expertise.Nous presentons dans ce document un outil modulaire d’annotation et de visualisation, GenoBrowser, que nous avons cree dans le cadre de nos travaux de recherche dans une equipe de microbiologie. Celui-ci nous permet d’integrer simplement de nouvelles fonctionnalites liees aux donnees de Omics generees dans l’equipe. L’architecture de notre outil et la creation d’une API (Application Programming Interface) specifique nous ont permis de developper et de mettre a la disposition de la communaute scientifique deux bases de donnees (P2CS et P2TF) dediees aux reseaux de regulation chez les bacteries, ainsi que le serveur web associe pour la prediction de ces systemes pour des genomes sequences de novo.Ce travail a permis de developper, au sein d’une equipe de recherche, un ensemble d’outils d’aide a l’expertise pour la recherche en genomique environnementale. Il nous a permis de travailler sur la consolidation et la reutilisation de la quantite croissante de donnees de type Omics.
Saccharomyces cerevisiae Rbg1 Protein and Its Binding Partner Gir2 Interact on Polyribosomes with Gcn1

ABSTRACT Rbg1 is a previously uncharacterized protein of Saccharomyces cerevisiae belonging to the Obg/CgtA subfamily of GTP-binding proteins whose members are involved in ribosome function in both prokaryotes and eukaryotes. We show here that Rbg1 specifically associates with translating ribosomes. In addition, in this study proteins were identified that interact with Rbg1 by yeast two-hybrid screening and include Tma46, Ygr250c, Yap1, and Gir2. Gir2 contains a GI (Gcn2 and Impact) domain similar to that of Gcn2, an essential factor of the general amino acid control pathway required for overcoming amino acid shortage. Interestingly, we found that Gir2, like Gcn2, interacts with Gcn1 through its GI domain, and overexpression of Gir2, under conditions mimicking amino acid starvation, resulted in inhibition of growth that could be reversed by Gcn2 co-overexpression. Moreover, we found that Gir2 also cofractionated with polyribosomes, and this fractionation pattern was partially dependent on the presence of Gcn1. Based on these findings, we conclude that Rbg1 and its interacting partner Gir2 associate with ribosomes, and their possible biological roles are discussed.
Implicit and Explicit Mechanisms of Word Learning in a Narrative Context: An Event-related Potential Study

The vast majority of word meanings are learned simply by extracting them from context rather than by rote memorization or explicit instruction. Although this skill is remarkable, little is known about the brain mechanisms involved. In the present study, ERPs were recorded as participants read stories in which pseudowords were presented multiple times, embedded in consistent, meaningful contexts (referred to as meaning condition, M+) or inconsistent, meaningless contexts (M−). Word learning was then assessed implicitly using a lexical decision task and explicitly through recall and recognition tasks. Overall, during story reading, M− words elicited a larger N400 than M+ words, suggesting that participants were better able to semantically integrate M+ words than M− words throughout the story. In addition, M+ words whose meanings were subsequently correctly recognized and recalled elicited a more positive ERP in a later time window compared with M+ words whose meanings were incorrectly remembered, consistent with the idea that the late positive component is an index of encoding processes. In the lexical decision task, no behavioral or electrophysiological evidence for implicit priming was found for M+ words. In contrast, during the explicit recognition task, M+ words showed a robust N400 effect. The N400 effect was dependent upon recognition performance, such that only correctly recognized M+ words elicited an N400. This pattern of results provides evidence that the explicit representations of word meanings can develop rapidly, whereas implicit representations may require more extensive exposure or more time to emerge.
Mucosal immunization with a flagellin-adjuvanted Hgp44 vaccine enhances protective immune responses in a murine Porphyromonas gingivalis infection model

ABSTRACT Chronic periodontitis is caused by interactions between the oral polymicrobial community and host factors. Periodontal diseases are associated with dysbiotic shift in oral microbiota. Vaccination against periodontopathic bacteria could be a fundamental therapeutic to modulate polymicrobial biofilms. Because oral cavity is the site of periodontopathic bacterial colonization, mucosal vaccines should provide better protection than vaccines administered systemically. We previously reported that bacterial flagellin is an excellent mucosal adjuvant. In this study, we investigated whether mucosal immunization with a flagellin-adjuvanted polypeptide vaccine induces protective immune responses using a Porphyromonas gingivalis infection model. We used the Hgp44 domain polypeptide of Arg-gingipain A (RgpA) as a mucosal antigen. Intranasal (IN) immunization induced a significantly higher Hgp44-specific IgG titer in the serum of mice than sublingual (SL) administration. The co-administration of flagellin potentiated serum IgG responses for both the IN and SL vaccinations. On the other hand, the anti-Hgp44-specific IgA titer in the saliva was comparable between IN and SL vaccinations, suggesting SL administration as more compliant vaccination route for periodontal vaccines. The co-administration of flagellin significantly potentiated the secretory IgA response in saliva also. Furthermore, mice administered a mixture of Hgp44 and flagellin via the IN and SL routes exhibited significant reductions in alveolar bone loss induced by live P. gingivalis infections. An intranasally administered Hgp44-flagellin fusion protein induced a comparable level of Hgp44-specific antibody responses to the mixture of Hgp44 and flagellin. Overall, a flagellin-adjuvanted Hgp44 antigen would serve an important component for a multivalent mucosal vaccine against polymicrobial periodontitis.
House Price Dynamics, Optimal LTV Limits and the Liquidity Trap

This paper studies the optimal design of a macro-prudential instrument, a loan-to-value (LTV) limit, and its implications for monetary policy in a model with nominal rigidities and financial frictions. The analysis accounts for both an effective lower bound on the nominal interest rate and an upper bound on the ability of LTV limits to stimulate credit demand. The welfare-based loss function features a role for macro-prudential policy to enhance risk-sharing. Optimal LTV limits are strongly countercyclical. In a house price boom-bust episode, the active use of LTV limits alleviates debt-deleveraging dynamics and prevents the economy from falling into a liquidity trap.
Integron Carrying a Novel Metallo-β-Lactamase Gene, blaIMP-16, and a Fused Form of Aminoglycoside-Resistant Gene aac(6′)-30/aac(6′)-Ib′: Report from the SENTRY Antimicrobial Surveillance Program

ABSTRACT Since January 2002 Pseudomonas sp. strains resistant to carbapenems and ceftazidime have been routinely screened as part of the SENTRY Antimicrobial Surveillance Program for metallo-β-lactamase production, and their resistance determinants have been analyzed. Pseudomonas aeruginosa index strain 101-4704, which harbors a novel blaIMP variant, blaIMP-16, was isolated in April 2002 from a 60-year-old man in Brasília, Brazil. blaIMP-16 was found on the chromosome of the P. aeruginosa index strain, and the deduced amino acid sequence (IMP-16) showed the greatest identities to IMP-11 (90.3%) and IMP-8 (89.5%). Sequence analysis revealed that blaIMP-16 was associated with a class 1 integron, which also encoded aminoglycoside-modifying enzymes. Downstream of blaIMP-16 resided an open reading frame, which consisted of a new aminoglycoside-modifying gene, namely, aac(6′)-30, which was fused with aac(6′)-Ib′. The amino acid sequence of the aac(6′)-30 putative protein showed the most identity (52.7%) to the sequence of AAC(6′)-29b described previously. The fourth gene cassette constituted aadA1. The steady-state kinetics of IMP-16 demonstrated that the enzyme preferred cephalosporins and carbapenems to penicillins. The main functional difference observed among the kinetic values for IMP-16 compared to those for other IMPs was a lack of cefoxitin hydrolysis and a lower kcat/Km value for imipenem (0.36 μM−1 · s−1). This report further emphasizes the spread of metallo-β-lactamase genes and their close association with various aminoglycoside resistance genes.
Do friends follow each other? : FDI network effects in Central Europe = Jönnek majd a baráti vállalatok? :FDI hálózati hatások Közép-Európában

A great deal of multinationals receive a bundle of hidden or cash subsidizes upon investing in a foreign country. Policymakers often argue that a subsidy today will help locate friends of the investor later on. Using extensive data on FDI investments, we analyze such patterns. In particular, we investigate if co-location is more frequent among connected firms such as members of business groups as well as firms sharing similar background. Focusing on investments into Central and Eastern European countries we find evidence of co-location pattern of connected firms.
Nucleosome Positioning in Saccharomyces cerevisiae

SUMMARY The DNA of eukaryotic cells is spooled around large histone protein complexes, forming nucleosomes that make up the basis for a high-order packaging structure called chromatin. Compared to naked DNA, nucleosomal DNA is less accessible to regulatory proteins and regulatory processes. The exact positions of nucleosomes therefore influence several cellular processes, including gene expression, chromosome segregation, recombination, replication, and DNA repair. Here, we review recent technological advances enabling the genome-wide mapping of nucleosome positions in the model eukaryote Saccharomyces cerevisiae. We discuss the various parameters that determine nucleosome positioning in vivo, including cis factors like AT content, variable tandem repeats, and poly(dA:dT) tracts that function as chromatin barriers and trans factors such as chromatin remodeling complexes, transcription factors, histone-modifying enzymes, and RNA polymerases. In the last section, we review the biological role of chromatin in gene transcription, the evolution of gene regulation, and epigenetic phenomena.
The morphological effects on the rat mandibular condyle of section of the lateral pterygoid muscle.

The effect of section of the lateral pterygoid muscle on the morphology of the mandible was studied in 32 ten-week-old Sprague-Dawley rats of both sexes. The rats were divided into four groups: control, sham-, unilaterally- and bilaterally-operated. Some of the rats received tetracycline after operation and alizarin before sacrifice. All the operated rats with section of the lateral pterygoid muscle showed resorption of the anterior border of the mandibular condyle and growth at the posterior border, confirmed by fluorescent microscopy. Both resorption and bone formation led to deformation of mandibular condyle.
Is deterrence approach effective in combating tax evasion? A meta-analysis

The purpose of this paper is to present the results of a meta-analysis of the relationship between determinant factors and tax evasion based on deterrence approach. Using the meta-analysis method, each statistical result of empirical studies is converted into r-pearson as standardized effect size, and then synthesized into a mean effect size in order to increase power and to resolve uncertainty. Theoretically, increasing audit, tax rate and tax penalty will decrease tax evasion. However, the results show that only tax rate has a significant impact on tax evasion. Synthesizing totally 478 outcomes from articles published between 1978 and 2018, there is a robust conclusion that decreasing tax rate is an effective tool in combating tax evasion. On the other hand, audit and penalty are not significant in influencing tax evasion. In addition, the results of heterogeneity analysis suggest that national culture and income level of the country are useful in explaining the impact of audit, tax rate and tax penalty on tax evasion. These findings should be of interest to policymakers. First, instead of sacrificing more resources in conducting audit or imposing more penalty, tax authorities should consider setting the tax rate as low as possible to diminish tax evasion. Second, considering that culture and income level influence the impact of audit and penalty on tax evasion, policymakers should consider national cultural values and income level condition when designing audit techniques and setting penalty structures.
Explaining Charter School Effectiveness

Lottery estimates suggest Massachusetts' urban charter schools boost achievement well beyond that of traditional urban public schools students, while nonurban charters reduce achievement from a higher baseline. The fact that urban charters are most effective for poor nonwhites and low-baseline achievers contributes to, but does not fully explain, these differences. We therefore link school-level charter impacts to school inputs and practices. The relative efficacy of urban lottery sample charters is accounted for by these schools' embrace of the No Excuses approach to urban education. In our Massachusetts sample, Non-No-Excuses urban charters are no more effective than nonurban charters
Inhibition of IκB Kinase Attenuates the Organ Injury and Dysfunction Associated with Hemorrhagic Shock

Nuclear factor-kappa B (NF-κB) activation is widely implicated in multiple organ failure (MOF); however, a direct inhibitor of IκB kinase (IKK), which plays a pivotal role in the activation of NF-κB, has not been investigated in shock. Thus, the aim of the present work was to investigate the effects of an IKK inhibitor on the MOF associated with hemorrhagic shock (HS). Therefore, rats were subjected to HS and were resuscitated with the shed blood. Rats were treated with the inhibitor of IKK or vehicle at resuscitation. Four hours later, blood and organs were assessed for organ injury and signaling events involved in the activation of NF-κB. Additionally, survival following serum deprivation was assessed in HK-2 cells treated with the inhibitor of IKK. HS resulted in renal dysfunction, lung, liver and muscular injury, and increases in serum inflammatory cytokines. Kidney and liver tissue from HS rats revealed increases in phosphorylation of IKKαβ and IκBα, nuclear translocation of NF-κB and expression of inducible isoform of nitric oxide synthase (iNOS). IKK16 treatment upon resuscitation attenuated NF-κB activation and activated the Akt survival pathway, leading to a significant attenuation of all of the above parameters. Furthermore, IKK16 exhibited cytoprotective effects in human kidney cells. In conclusion, the inhibitor of IKK complex attenuated the MOF associated with HS. This effect may be due to the inhibition of the NF-κB pathway and activation of the survival kinase Akt. Thus, the inhibition of the IKK complex might be an effective strategy for the prevention of MOF associated with HS.
Complex Flow Image Velocimetry in Shock Instabilities with Fractal Boundaries

We use an advanced version of Correlation Particle Image Velocimetry used in surface flows SFIV [1,2] in order to analyze the complex patterns due to the basic instabilities and boundary conditions, and to relate the production and detection of vortices, advected by fast flows with cores of low pressure. These coincide with the 3D lines of strong vorticity or helicity. For example in fast flowing rivers or laboratory experiments of environmental hydraulics [3] or shocks in compressible mixing [4]. The mixing fronts interacts with a density interface producing positive or negative baroclinic structures with varying turbulent cascades[5]. LIF Images of the thickness of the mixing zone at the centre of a shock tube, allow us to perform Multi-Fractal analysis on the evolution of the interfaces [6,7]. The interactions of the pressure fronts with balloons filled with various density gas also allow a wide range of initial conditions. In the same way, using wakes of fractal grids also modify the cascade proceses[7,8]. The three-dimensional mixing zone, its thickness and topology are important experimental measurements. The three basic cases are: when the shock wave passes from a heavy gas to a light one; from a gas to another of similar densities and from a light gas to a heavy one.
Selection and Combination of Unsupervised Learning Methods for Image Retrieval

The evolution of technologies to store and share images has made imperative the need for methods to index and retrieve multimedia information based on visual content. The CBIR (Content-Based Image Retrieval) systems are the main solution in this scenario. Originally, these systems were solely based on the use of low-level visual features, but evolved through the years in order to incorporate various supervised learning techniques. More recently, unsupervised learning methods have been showing promising results for improving the effectiveness of retrieval results. However, given the development of different methods, a challenging task consists in to exploit the advantages of diverse approaches. As different methods present distinct results even for the same dataset and set of features, a promising approach is to combine these methods. In this work, a framework is proposed aiming at selecting the best combination of methods in a given scenario, using different strategies based on effectiveness and correlation measures. Regarding the experimental evaluation, six distinct unsupervised learning methods and two different datasets were used. The results as a whole are promising and also reveal good perspectives for future works.