Miracle-dz/UniFAR-FTUs huggingface dataset:
RPTP&agr; controls epithelial adherens junctions, linking E-cadherin engagement to c-Src-mediated phosphorylation of cortactin

ABSTRACT Epithelial junctions are fundamental determinants of tissue organization, subject to regulation by tyrosine phosphorylation. Homophilic binding of E-cadherin activates tyrosine kinases, such as Src, that control junctional integrity. Protein tyrosine phosphatases (PTPs) also contribute to cadherin-based adhesion and signaling, but little is known about their specific identity or functions at epithelial junctions. Here, we report that the receptor PTP RPTP&agr; (human gene name PTPRA) is recruited to epithelial adherens junctions at the time of cell–cell contact, where it is in molecular proximity to E-cadherin. RPTP&agr; is required for appropriate cadherin-dependent adhesion and for cyst architecture in three-dimensional culture. Loss of RPTP&agr; impairs adherens junction integrity, as manifested by defective E-cadherin accumulation and peri-junctional F-actin density. These effects correlate with a role for RPTP&agr; in cellular (c)-Src activation at sites of E-cadherin engagement. Mechanistically, RPTP&agr; is required for appropriate tyrosine phosphorylation of cortactin, a major Src substrate and a cytoskeletal actin organizer. Expression of a phosphomimetic cortactin mutant in RPTP&agr;-depleted cells partially rescues F-actin and E-cadherin accumulation at intercellular contacts. These findings indicate that RPTP&agr; controls cadherin-mediated signaling by linking homophilic E-cadherin engagement to cortactin tyrosine phosphorylation through c-Src.
Temporal Effects on Hashtag Reuse in Twitter: A Cognitive-Inspired Hashtag Recommendation Approach

Hashtags have become a powerful tool in social platforms such as Twitter to categorize and search for content, and to spread short messages across members of the social network. In this paper, we study temporal hashtag usage practices in Twitter with the aim of designing a cognitive-inspired hashtag recommendation algorithm we call BLLi,s. Our main idea is to incorporate the effect of time on (i) individual hashtag reuse (i.e., reusing own hashtags), and (ii) social hashtag reuse (i.e., reusing hashtags, which has been previously used by a followee) into a predictive model. For this, we turn to the Base-Level Learning (BLL) equation from the cognitive architecture ACT-R, which accounts for the time-dependent decay of item exposure in human memory. We validate BLLI,S using two crawled Twitter datasets in two evaluation scenarios. Firstly, only temporal usage patterns of past hashtag assignments are utilized and secondly, these patterns are combined with a content-based analysis of the current tweet. In both evaluation scenarios, we find not only that temporal effects play an important role for both individual and social hashtag reuse but also that our BLLI,S approach provides significantly better prediction accuracy and ranking results than current state-of-the-art hashtag recommendation methods.
Polysilsesquioxane nanoparticles for targeted platin-based cancer chemotherapy by triggered release.

Since the discovery of cisplatin by Rosenberg et al. in the 1960s, platinum anticancer drugs have played an important role in cancer chemotherapy. [1] Cisplatin is used to treat a variety of cancers such as testicular, lung, breast, and ovarian. The success of cisplatin has led to the synthesis and biological evaluation of thousands of platinum complexes; however, only two additional complexes, carboplatin and oxaliplatin, are approved for clinical use by the FDA. [2] The clinical efficacy of platinum-based anticancer drugs is limited by their poor pharmacokinetic properties. High doses of platins are needed, which often leads to severe side effects. Furthermore, many tumors display inherent or acquired resistance to platinum-based therapies. There exists a need to develop alternative strategies to effectively deliver platinum drugs to the tumor, with fewer side effects.
Down-Regulation of TRPM8 in Pulmonary Arteries of Pulmonary Hypertensive Rats

Background: Pulmonary hypertension (PH) is characterized by profound vascular remodeling and alterations in Ca2+ homeostasis in pulmonary arterial smooth muscle cells (PASMCs). Multiple transient receptor potential melastatin-related (TRPM) subtypes have been identified in vascular tissue. However, the changes in the expression and function of TRPM channels in pulmonary hypertension have not been characterized in detail. Methods: We examined the expression of TRPM channels and characterized the functions of the altered TRPM channels in two widely used rat models of chronic hypoxia (CH)- and monocrotaline (MCT)-induced PH. Results: CH-exposed and MCT-treated rats developed severe PH and right ventricular hypertrophy, with a significant decrease in TRPM8 mRNA and protein expression in pulmonary arteries (PAs). The downregulation of TRPM8 was associated with significant reduction in menthol-induced cation-influx. Time-profiles showed that TRPM8 down-regulation occurred prior to the increase of right ventricular systolic pressure (RVSP) and right ventricular mass index (RVMI) in CH-exposed rats, but these changes were delayed in MCT-treated rats. The TRPM8 agonist menthol induced vasorelaxation in phenylephrine-precontracted PAs, and the vasorelaxing effects were significantly attenuated in PAs of both PH rat models, consistent with decreased TRPM8 expression. Conclusion: Downregulation of TRPM8 may contribute to the enhanced vasoreactivity in PH.
Best N-term approximation in electronic structure calculations. II. Jastrow factors

We present a novel application of best N-term approximation theory in the framework of electronic structure calculations. The paper focusses on the description of electron correlations within a Jastrow-type ansatz for the wavefunction. As a starting point we discuss certain natural assumptions on the asymptotic behaviour of two-particle correlation functions F (2) near electron-electron and electron- nuclear cusps. Based on Nitsche's characterization of best N-term approximation spaces A α (H 1 ), we prove that F (2) ∈ A α (H 1 )f or q> 1a ndα = 1 − 1 with respect to a certain class of anisotropic wavelet tensor product bases. Computational arguments are given in favour of this specific class compared to other possible tensor product bases. Finally, we compare the approximation properties of wavelet bases with standard Gaussian-type basis sets frequently used in quantum chemistry. Mathematics Subject Classification. 41A50, 41A63, 65Z05, 81V70.
The mannose-binding lectin gene polymorphisms and systemic lupus erythematosus: two case-control studies and a meta-analysis.

OBJECTIVE Mannose-binding lectin (MBL) enhances opsonization and activates complement. Dysfunctional alleles of MBL have been associated with low plasma concentrations of MBL and increased risk of systemic lupus erythematosus (SLE), but genotyping studies have shown inconsistent results. We performed case-control studies of the MBL polymorphisms in 2 Caucasian cohorts and a meta-analysis incorporating all published results of MBL genotyping in SLE to explore whether the MBL functional variants are associated with SLE. METHODS MBL genotypes at 7 single-nucleotide polymorphisms were sequenced in 96 European American patients with SLE and 96 age-, race-, and sex-matched controls. MBL codons 52, 54, and 57 were genotyped in 285 German patients with SLE and 200 race-matched controls. Allele frequencies of all known variants were tallied for meta-analysis. RESULTS Although there was a trend toward association with MBL polymorphisms in both patient cohorts evaluated, none of them was significantly associated with SLE on its own. Seventeen comparisons from 15 studies were included in the meta-analysis. Publication bias was excluded by Egger's regression test (P = 0.14). The overall odds ratio for MBL codon 54 variant B was 1.406 (95% confidence interval 1.221-1.608; P < 0.001). Stratification by ethnicity showed significantly increased odds ratios for association of the MBL codon 54 B variant with SLE in African, Asian, and Caucasian cohorts. CONCLUSION Meta-analysis of all available studies on MBL polymorphisms and SLE shows that MBL variant alleles such as MBL exon 1 codon 54 B, promoter -550 L, and promoter -221 X are SLE risk factors. This association is robust and persists after incorporation of data from our 2 cohorts in which the association failed to reach significance.
The reciprocal influence of organizational culture and training and development programs

Purpose – The purpose of this paper is to demonstrate that training designers can, and should, account for organizational culture during training needs assessments.Design/methodology/approach – Utilizing the approach and arguments in Giddens' structuration theory, the paper conceptually applies these tenets to training and development programs within organizations.Findings – Within a typical analysis‐design‐develop‐implement‐evaluate (ADDIE) training model, relationships between organizational culture and each step of the training are conceptually available. Organizational culture shapes, influences, and redefines training programs which, in turn, shape, influence, and redefine organizational culture. Including a culture analysis within program planning will ultimately alleviate many of the problems that may arise during the implementation of a training and development program because of cultural resistance and/or clash of values between culture and training.Research limitations/implications – The argumen.
A Cooperative Method of SFM and Stereo for Motion and Depth Extraction

method is robust enough to be used in industry and society. Stereo is a useful technique for obtaining depth information from images. However, it is required that the baseline length between two cameras should be large to increase depth precision. Consequently, stereo matching suffers from ambiguities due to large baseline length. Therefore, the matching problem would become much easier if a sequence of densely sampled images along a camera path is used. "Structure from Motion (SFM)" do not suffer from the matching problem because many SFM algorithms use multiple images, especially a sequence of images taken at short time intervals. However, this method basically has one disadvantage of having scale factor ambiguity. The depth extraction method proposed in this paper integrates SFM and stereo depth extraction algorithm to determine a scale factor for SFM and to solve the matching problem of stereo even if there are ambiguities in matching and occlusion regions in the scene. Results on real images illustrate the performance of the proposed method.
A Medium Access Control Protocol with Adaptive Parent Selection Mechanism for Large-Scale Sensor Networks

In the MAC protocols based on the S-MAC scheme, usually the combination of periodic sleep/listen scheduling and four-way handshake mechanism is employed to reduce idle listening and avoid interference. However, this combination greatly degrades network capacity and results in high end-to-end latency. In this paper, we propose Adaptive IAMAC to increase channel utilization and improve communication efficiency, specifically in large-scale sensor networks with low duty cycle. Adaptive IAMAC allows multiple nodes to transmit to their common parent during a frame. Moreover, it includes the adaptive parent selection mechanism, which enables the nodes to change their parent according to the currently overheard control packets at the MAC layer. Through these techniques, Adaptive IAMAC enhances network throughput, reduces end-to-end latency, and moderates the overhead of four-way handshake mechanism. Simulation results confirm that Adaptive IAMAC provides significant improvements over S-MAC in terms of throughput, latency, and energy efficiency.
SISTEM PENJADWALAN IKLAN PADA RADIO MEGANTARA FM NGANJUK DENGAN ALGORITMA DYNAMIC PRIORITY SCHEDULLING

Dengan berkembangnya Teknologi Informasi saat ini, menimbulkan banyaknya layanan penyedia informasi. Salah satunya adalah media Radio. Salah satu aktifitas produksi dari media Radio adalah penyiaran iklan. Dalam proses penyiaran iklan, suatu acara yang telah tersusun akan disisipi iklan-iklan dan dijadwalkan untuk disiarkan. Iklan sebelum disisipkan penyiarannya pada suatu acara, terlebih dahulu harus diketahui akan disiarkan pada acara yang mana, kapan waktu siarnya, dan berapa kali iklan harus disiarkan. Dengan mengadopsi suatu metode penjadwalan pada Sistem Operasi, yaitu Dynamic Priority Schedulling dibuat suatu jadwal sebagai kartu acara, yang didalamnya berisi kapan (waktu) acara harus dipotong untuk bisa diisi iklan, berapa kali acara bisa dipotong. Metode ini mengatur suatu penjadwalan yang dalam pelaksanaannya, proses akan dijadwalkan untuk dilaksanakan sesuai dengan prioritas proses tersebut. Jadi semua iklan, akan mempunyai tingkatan prioritas dan akan dikerjakan berurutan sesuai dengan prioritasnya. Dengan tujuan mengefektifkan sumber daya yang terkait. Dengan adanya aplikasi ini diharapkan dapat membantu dalam proses pelaksanaan administrasi, terutama pada bagian pemasaran Iklan dan Produksi.
LodLive, exploring the web of data

LodLive project, http://en.lodlive. it/, provides a demonstration of the use of Linked Data standard (RDF, SPARQL) to browse RDF resources. The application aims to spread linked data principles with a simple and friendly interface and reusable techniques. In this report we present an overview of the potential of LodLive, mentioning tools and methodologies that were used to create it.
Improvements on Visual Servoing From Spherical Targets Using a Spherical Projection Model

This paper is concerned with improvements to visual feature modeling using a spherical projection model. Three spherical targets are considered: a sphere, a sphere marked with a tangent vector to a point on its surface, and a sphere marked with two points on its surface. A new minimal and decoupled set of visual features is proposed for each target using any central catadioptric camera. Using the proposed set for a sphere, a classical control law is proved to be globally asymptotically stable in the presence of modeling errors and locally asymptotically stable in the presence of calibration errors, considering that perspective and paracatadioptric cameras were used. Simulation and experimental results with perspective, paracatadioptric, and fish-eye cameras validate the proposed theoretical results.
MEDIDA NA AVALIAÇÃO PSICOLÓGICA

The present study discusses the role of measurement in psychological assessment, criticising the abuse in the use of single numbers, as well as complex statistics with inadequate. At its inception, psychological assessment did not use complex statistics. The study defends that psychologists must base their procedures on psychological models, rather than mathematical models. We state that starting in the 1980's, when validity became a unified concept, a combination of different and integrated procedures including - eventually- statistics, started being utilized. We argue that at a time when much of the psychological techniques and procedures originally developed by psychology are used by non-psychologists, special care with the use of psychological assessment tools should be taken, including the choice of evaluation techniques, their application, and in particular regarding the interpretation and use of results, which must be guided by the rules and ethical principles of psychology. It is this informed sequence which makes psychological evaluation an instrument that needs to be applied by psychologists. Many researchers, professionals, and fields use techniques and instruments originally developed by, or following the principles of psychology, but that does not in itself constitute psychological assessment.
A texture index for evaluating accounting narratives: An alternative to readability formulas

Readability formulas have been criticised as a method for scoring accounting narratives because of their focus on word‐ and sentence‐level features and not on whole‐text aspects, their lack of regard for the interests and motivation of the reader, and their inappropriateness for evaluating adult‐based and technical accounting narratives. The literature of linguistics offers theoretical and practical validation for application of a texture index which addresses these criticisms. The paper shows how the general model drawn from applied linguistics can be tailored to the specific situation of an accounting narrative – the Operating and Financial Review. Rules which provide for objectivity in replication are specified and illustrated for a sample narrative. Illustrative empirical analysis shows that there is no evidence of association with the Flesch readability score. This suggests that the texture index is potentially a powerful tool for analysis of accounting narratives and association testing.
Improvement in reliability by changing the deterministic inputs of nanoscale circuits

Scaling of CMOS technology is degrading the reliability of upcoming microelectronic devices. When the circuit design enters the nanoscale dimensions, the inputs have more influence on the circuit's reliability due to the circuit's internal noises and gate errors. In this paper, we will model the deterministic inputs probabilistically and analyze their effect on the reliability of digital circuits. The analysis is based on the Bayesian networks error modelling scheme. The simulations are based on MATLAB and show the important relationships among different deterministic inputs and their reliabilities. The results show the range of reliability values obtained by changing the deterministic input probability values.
Metodología en la investigación transcultural: perspectivas actuales

El desarrollo de una metodologia especifica para la realizacion de estudios transculturales tiene como objetivo responder a una serie de problemas a los que el in vestigador se enfrenta cuando pretende com parar g rupos culturales. El proceso de operacionalizar el constructo cultura en sus indicadores, debe realizarse atendiendo a tres etapas (van de Vijver y Leung, 1997b): la primera se refiere a la eleccion de las variables contextuales que permitan interpretar de forma adecuada las diferencias culturales; la segunda tiene que ver con el diseno del estudio en funcion del cual se realizara la medicion de las v ariables contextuales y, finalmente, la tercera etapa hace referencia a la estr ategia de analisis estadistico requerida para evaluar en que medida las varia bles contextuales explican las diferencias transcultu rales. En este sentido, la investigacion transcultural se enfrenta al control de una serie de sesgos que, si bien son comunes a toda in vestigacion, presentan caracteristicas especificas en este contexto. El ob jetivo del presente tr abajo consiste en presentar las principales fuentes de sesgo a las que se enfrenta la investigacion transcultural, asociadas basicamente a los materiales y al procedimiento utilizados para la reco gida de los datos asi como a los diferentes estilos de respuesta que presentan los sujetos en las distintas culturas. Asimismo, abordaremos las principales estr ategias de muestreo y de analisis estadistico que se utilizan en la investigacion transcultural, tomando como referencia una propuesta de clasificacion de los estudios transculturales en funcion de dimensiones que condicionan la for mulacion de hipotesis y el metodo utilizado en el estudio.
Kinetic analysis of open‐ and closed‐state inactivation transitions in human Kv4.2 A‐type potassium channels

1 We studied the gating kinetics of Kv4.2 channels, the molecular substrate of neuronal somatodendritic A‐type currents. For this purpose wild‐type and mutant channels were transiently expressed in the human embryonic kidney (HEK) 293 cell line and currents were measured in the whole‐cell patch‐clamp configuration. 2 Kv4.2 channels inactivated from pre‐open closed state(s) with a mean time constant of 959 ms at ‐50 mV. This closed‐state inactivation was not affected by a deletion of the Kv4.2 N‐terminus (Δ2‐40). 3 Kv4.2 currents at +40 mV inactivated with triple‐exponential kinetics. A fast component (τ= 11 ms) accounted for 73 %, an intermediate component (τ= 50 ms) for 23 % and a slow component (τ= 668 ms) for 4 % of the total decay. 4 Both the fast and the intermediate components of inactivation were slowed by a deletion of the Kv4.2 N‐terminus (τ= 35 and 111 ms) and accounted for 33 and 56 %, respectively, of the total decay. The slow component was moderately accelerated by the truncation (τ= 346 ms) and accounted for 11 % of the total Kv4.2 current inactivation. 5 Recovery from open‐state inactivation and recovery from closed‐state inactivation occurred with similar kinetics in a strongly voltage‐dependent manner. Neither recovery reaction was affected by the N‐terminal truncation. 6 Kv4.2 Δ2‐40 channels displayed slowed deactivation kinetics, suggesting that the N‐terminal truncation leads to a stabilization of the open state. 7 Simulations with an allosteric model of inactivation, supported by the experimental data, suggested that, in response to membrane depolarization, Kv4.2 channels accumulate in the closed‐inactivated state(s), from which they directly recover, bypassing the open state.
HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages

HIV-1 assembly and release are believed to occur at the plasma membrane in most host cells with the exception of primary macrophages, for which exclusive budding at late endosomes has been reported. Here, we applied a novel ultrastructural approach to assess HIV-1 budding in primary macrophages in an immunomarker-independent manner. Infected macrophages were fed with BSA-gold and stained with the membrane-impermeant dye ruthenium red to identify endosomes and the plasma membrane, respectively. Virus-filled vacuolar structures with a seemingly intracellular localization displayed intense staining with ruthenium red, but lacked endocytosed BSA-gold, defining them as plasma membrane. Moreover, HIV budding profiles were virtually excluded from gold-filled endosomes while frequently being detected on ruthenium red–positive membranes. The composition of cellular marker proteins incorporated into HIV-1 supported a plasma membrane–derived origin of the viral envelope. Thus, contrary to current opinion, the plasma membrane is the primary site of HIV-1 budding also in infected macrophages.
Genetic mapping of mutations in independently isolated nontoxinogenic mutants of Vibrio cholerae

Conjugal mating experiments were performed between donor strains of Vibrio cholerae carrying the vibrio sex plasmid P and recipient strains lacking the P plasmid. Donor and recipient genotypes differed with respect to toxinogenicity (tox), nutritional requirements, and antibiotic susceptibilities. Recombinants carrying selected donor and recipient markers were produced at low frequencies in conjugal matings. Mapping of tox markers was accomplished by scoring for the frequency of coinheritance of the donor tox allele with selected and unselected donor markers. Four independently isolated tox markers were analyzed. Each of these four tox markers was shown to be linked to the his-1 site in linkage group I on the genetic map of V. cholerae. In matings between a recipient strain carrying the tox-1 marker and donor strains carrying either tox-2, tox-3, or tox-4, all selected his+ recombinants remained nontoxinogenic. Matings between multiply marked strains demonstrated that the position of tox-1 with respect to other genetic loci in linkage group I is as follows: met-2--trp-1--asp-1--nal-1--his-1--tox-1. These findings demonstrate that a chromosomal determinant linked to his-1 in linkage group I on the genetic map of V. cholerae is essential for toxinogenesis and suggest that tox-1,tox-2, tox-3, and tox-4 may be alleles of a single tox gene.