Miracle-dz/UniFAR-FTUs huggingface dataset:
Resolution and identification of iron-containing antigens in membrane vesicles from Escherichia coli.

Iron-containing antigens present in membrane vesicles prepared from Escherichia coli ML 308-225 were analyzed by crossed immunoelectrophoresis following growth of the organism in the presence of 59Fe. Seven discrete antigens (or antigen complexes) are detected by autoradiography, and six contain primarily nonheme iron. Three immunoprecipitates are positively identified as NADH dehydrogenase (EC 1.6.99.3), NADPH dehydrogenase (EC 1.6.99.1), and glutamate dehydrogenase (EC 1.4.1.4) based on activity stains for these enzymes. Two other immunogens containing nonheme iron correspond to antigens no. 22 and 37 in the crossed immunoelectrophoresis reference pattern of Owen & Kaback [Owen, P., & Kaback, H. R. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 3148]. In addition, the immunoprecipitate corresponding to Braun's lipoprotein contains tightly bound iron.
Transcriptional Control of ADH Genes in the Xylose-Fermenting Yeast Pichia stipitis

ABSTRACT We studied the expression of the genes encoding group I alcohol dehydrogenases (PsADH1 and PsADH2) in the xylose-fermenting yeast Pichia stipitis CBS 6054. The cells expressed PsADH1 approximately 10 times higher under oxygen-limited conditions than under fully aerobic conditions when cultivated on xylose. Transcripts of PsADH2 were not detectable under either aeration condition. We used aPsADH1::lacZ fusion to monitorPsADH1 expression and found that expression increased as oxygen decreased. The level of PsADH1 transcript was repressed about 10-fold in cells grown in the presence of heme under oxygen-limited conditions. Concomitantly with the induction ofPsADH1, PsCYC1 expression was repressed. These results indicate that oxygen availability regulates PsADH1expression and that regulation may be mediated by heme. The regulation of PsADH2 expression was also examined in other genetic backgrounds. Disruption of PsADH1 dramatically increasedPsADH2 expression on nonfermentable carbon sources under fully aerobic conditions, indicating that the expression ofPsADH2 is subject to feedback regulation under these conditions.
Un début différent : étude de l’adaptation psychologique du couple à la parentalité et au diagnostic prénatal d’une pathologie fœtale, de la grossesse à la première année de vie de l’enfant

La transition vers la parentalite est une periode importante du developpement de la personne et de la famille. Devenir parent est un projet a long-terme qui engage la reconstruction des roles psychologiques et sociaux. Plusieurs elements sont censes influencer la facon dont les parents integrent cette nouvelle realite et s’adaptent au contexte psychosocial de la parentalite. Ainsi, il est important de comprendre les mecanismes selon lesquels les facteurs individuels et dyadiques contribuent a l'adaptation psychologique des couples ainsi que d’evaluer les conditions qui peuvent representer un risque lors de cette transition developpementale. Le diagnostic d’une pathologie fœtale pendant la grossesse est une situation singuliere dans le contexte de la parentalite, elle a jusqu’a present recu peu d’attention. Le stress inherent a cette situation tres exigeante, voire traumatogene pendant la periode perinatale, augmente le risque de difficultes emotionnelles et relationnelles et peut serieusement troubler le developpement et le fonctionnement des systemes familiaux.Dans l’objectif d’etudier les processus d’adaptation psychologique des couples a la parentalite, notamment suite a un diagnostic prenatal d’une pathologie, nous nous sommes dans un premier temps interessees a l’evolution psychologique « normale » de la grossesse et de la periode postnatale. Dans un deuxieme temps, axees sur le meme protocole de recherche, nous avons traite le parcours d’adaptation psychologique des couples ayant recu le diagnostic d’une pathologie chez le bebe des l’annonce et jusqu’a douze mois apres l’accouchement.Ce travail devrait permettre une meilleure connaissance des facteurs de risque et de protection de l’adaptation psychologique des parents et de la famille pendant la transition vers la parentalite et suite au diagnostic prenatal d’une pathologie fœtale. Au niveau clinique, cette etude permettra d’accroitre les connaissances relatives aux aspects du fonctionnement du couple auxquels les soignants doivent etre attentifs pendant la transition vers la parentalite et, en particulier, suite au diagnostic d’une pathologie fœtale afin de promouvoir l’adaptation psychologique du couple.
RET gene rearrangements (RET/PTC1 and RET/PTC3) in papillary thyroid carcinomas from an iodine‐rich country (Japan)

The frequency of RET rearrangements (RET/PTC) in papillary thyroid carcinomas varies significantly according to geographic area, with the greatest incidence reported in the Belarus region, which is iodine‐deficient and was contaminated severely after the Chernobyl reactor accident, and with the lowest incidence in iodine‐rich, nonirradiated Japan. The authors investigated the prevalence of RET/PTC in a large number of thyroid tumors from Japanese patients.
COMPARACIÓN DE LA SATISFACCIÓN LABORAL DEL DIRECTOR ESCOLAR Y LOS DOCENTES

Este articulo es producto de un proyecto de investigacion que tuvo como finalidad el comparar el grado de satisfaccion laboral del director escolar con el del docente. Se evaluaron diferentes aspectos relacionados a la satisfaccion laboral tales como: genero, edad, preparacion academica, anos de experiencia, deberes, autoridad, recursos, desarrollo profesional y ambiente institucional. El estudio se llevo a cabo en diferentes escuelas secundarias localizadas en el este de Orlando, Florida en los Estados Unidos de America. Una muestra de 293 docentes y 37 directores escolares fue seleccionada para participar en el estudio. La coleccion de datos fue mediante revision bibliografica, cuestionarios y entrevistas. Ademas, se utilizo la escala Likert para la cuantificacion de los resultados. Los hallazgos de esta investigacion reflejaron los niveles de satisfaccion e insatisfaccion existentes en directores escolares y docentes. Los aspectos de mayor importancia fueron la Autoridad, los Recursos, el Desarrollo Profesional y el Ambiente Institucional, ya que se encontro que la falta de comunicacion, el pobre liderazgo para la toma de decisiones, el salario y la falta de recursos fueron la causa primordial del descontento e insatisfaccion tanto en directores como en docentes. Como resultado del analisis realizado, una serie de recomendaciones fueron planteadas con el proposito de mejorar el desarrollo profesional y ambiente institucional, asi como unas condiciones laborales que fomenten un proceso de ensenanza-aprendizaje de calidad. Cabe recalcar, que este estudio servira para alentar a los miembros de la jerarquia educativa a encontrar medidas que fomenten un ambiente laboral que incite a docentes y directores a sentir una mayor satisfaccion con la labor que realizan, para asi lograr una educacion de excelencia. La satisfaccion del personal educativo fomentara un crecimiento en los diferentes aspectos de la educacion, ya que la satisfaccion laboral juega un papel importantisimo en el desempeno y la productividad de un individuo dentro de una organizacion.
An all atom force field for simulations of proteins and nucleic acids

We present an all atom potential energy function for the simulation of proteins and nucleic acids. This work is an extension of the CH united atom function recently presented by S.J. Weiner et al. J. Amer. Chem. Soc. , 106, 765 (1984). The parameters of our function are based on calculations on ethane, propane, n−butane, dimethyl ether, methyl ethyl ether, tetrahydrofuran, imidazole, indole, deoxyadenosine, base paired dinucleoside phosphates, adenine, guanine, uracil, cytosine, thymine, insulin, and myoglobin. We have also used these parameters to carry out the first general vibrational analysis of all five nucleic acid bases with a molecular mechanics potential approach.
Prediction‐based survivable virtual network mapping against disaster failures

Survivable virtual network mapping (SVNM) guarantees that the mapped virtual network works normally against substrate failures. Most of the existing solutions are mainly focusing on single node or single link failure. A long‐standing challenge in SVNM is to reduce the capacity loss of network when substrate failures happen. Because some regions are frequently attacked by disasters, the disaster failures should be paid attention to. In this paper, we re‐consider the existing work on the SVNM and explore the feasible solution of SVNM against disaster failures. We first design the disaster failure model with the knowledge of risk assessment. Then we formulate the problem with the mixed integer programming. Two heuristic algorithms based on the prediction mechanism are proposed. Simulations show that our algorithms increase the average acceptance ratio and reduce the risk of capacity loss in the initial mapping phase compared with previous algorithms. Copyright © 2016 John Wiley & Sons, Ltd.
Investigating the role of early childhood abuse and HPA axis genes in suicide attempters with bipolar disorder

Objective Genes and the environment both play a major role in the risk for attempted suicide, and environments harboring stressors, such as early childhood abuse, have been linked to suicidal behavior. Such environments also disrupt the hypothalamic–pituitary–adrenal (HPA) axis pathway, which has been hypothesized to play a role in suicidal behavior. We investigated whether the risk for attempted suicide was attributable in part to the interaction between childhood physical and/or sexual abuse and genetic variation in 19 genes (±5 kb) integral to the HPA axis pathway. Materials and methods Using the Genetic Association Information Network Bipolar Disorder and Translational Genomics Research Institute cohorts, we implemented PLINK’s logistic regression-based ‘interaction’ approach to search for evidence of an interaction between 235 genotyped HPA axis single-nucleotide polymorphisms and early childhood abuse. Our study included 631 bipolar disorder suicide attempters and 657 bipolar disorder nonattempters with information on abuse. Results After correction for multiple testing, no significant interaction between the 235 HPA axis single-nucleotide polymorphisms and early childhood abuse was found. In our study, the strongest interaction was found with rs2664008 in the corticotropin-releasing hormone receptor 1 (CRHR1) gene, with a nominal interaction P-value of 1.22×10−2 and an interaction odds ratio of 0.47. Conclusion Our findings suggest that further work and larger sample sizes are required to elucidate the link between early childhood abuse and the HPA axis in suicidal behavior.
The Production of English Aspirated Stops in Foreign Language Acquisition

This study examines the effect of teaching on the production of L2 English aspirated stops among Greek EFL (English as a Foreign Language) learners. Two groups of speakers were recorded, an experimental group who received the pronunciation teaching intervention and a control group who did not receive special pronunciation instruction. The data were analysed acoustically and a native-likeness criterion (Birdsong 2007, Flege et al. 1992) was used to investigate individual variability. Overall, this study reports a positive effect of pronunciation instruction on the production of aspirated stops, however the great individual variability suggests that systematic target-like production of stops constitutes a challenging goal. A tentative effect of learners’ gender is also obtained, as the female speakers produce greater VOT than the male ones after teaching.
THE INFLUENCE OF FAMILIARIZATION ON THE RELIABILITY OF FORCE VARIABLES MEASURED DURING UNLOADED AND LOADED VERTICAL JUMPS

The purpose of this study was to determine the number of familiarization sessions required to obtain an accurate measure of reliability associated with force variables recorded during unloaded and loaded (30 and 60% of 1 repetition maximum squat [1RM]) static vertical jumps (SJ). Nine physically active men attended 4 separate testing sessions over a 2-week period. Force platform recordings of peak force, peak rate of force development (pRFD), average rate of force development, takeoff velocity, average power, and peak power were obtained for each jump. During each of the 4 testing sessions, 3 jumps were performed under each of the load conditions. The average of the force variables were used in the analysis. Familiarization was assessed using the scores obtained during the 4 separate testing sessions. Reliability was assessed by calculating intraclass correlation coefficients (ICCs) and coefficient of variation (CV) associated with the force variables. No significant differences (p > 0.05) were obtained between the testing sessions for any of the force variables. With the exception of pRFD, the force variables showed reasonably good levels of test-retest reliability (ICC range: 0.75–0.99; CV range: 1.2–7.6%). High levels of reliability can be achieved in a variety of force variables without the need for familiarization sessions when performing SJ under unloaded conditions and with loads of 30 and 60% of 1RM squat with physically active men.
Measuring the number of hadronic jets.

A quantitative description of the qualitative feature of multihadron final states known as the number of jets'' is given by a sequence of infrared finite shape observables (jet discriminators) that take continuous values between 0 and 1, are stable--unlike clustering algorithms--against small variations of the input (data errors, Sudakov effects etc.), and have a form of multiparticle correlators that is natural in the context of quantum field theory and hence are better suited for a systematic study of theoretical uncertainties (logarithmic and power corrections).
Resting functional connectivity in patients with brain tumors in eloquent areas

Resection of brain tumors adjacent to eloquent areas represents a challenge in neurosurgery. If maximal resection is desired without inducing postoperative neurological deficits, a detailed knowledge of the functional topography in and around the tumor is crucial. The aim of the present work is to evaluate the value of preoperative magnetoencephalography (MEG) imaging of functional connectivity to predict the results of intraoperative electrical stimulation (IES) mapping, the clinical gold standard for neurosurgical localization of functional areas.
Data-Intensive Research Theme

• Malcolm Atkinson, School of Informatics & Director of eSI, University of Edinburgh, UK; • Martin Kersten, Centrum Wiskunde & Informatica (CWI), The Netherlands; • Alexander Szalay, School of Astronomy & Director of Institute for Data-Intensive Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and • Jano van Hemert, School of Informatics & National e-Science Centre, University of Edinburgh.
The Unintended Effects of Risk‐Refuting Information on Anxiety

Researchers in the field of risk perception have been asking why people are more worried about risk today than in years past. This article explores one possible answer to this question, associative anxiety. The affect heuristic and the mental network models suggest that anxiety triggered by information regarding a particular risk can spread to other risks of the same category. Research to date, however, has not examined how information refuting the particular risk can also be generalized across other risks. The article presents two experimental studies addressing this issue. Study 1 showed that when participants were presented with information based on a real train collision, they experienced increased anxiety not only about train collisions but also about public transportation in general. In contrast, those who were informed about the train collision case as well as the preventative measures implemented after the accident experienced decreased anxiety about train collisions but not about public transportation more generally. Study 2 measured the changes in participant anxiety about a genetically modified organism (GMO) and compared the influence of information about either the existence or nonexistence of its risk. Similar to Study 1, associative anxiety rippled through the risk category. The results also suggest that the follow‐up information refuting the GMO risk reduced the anxiety toward the hazard drastically, but did not fully alleviate the anxiety toward other hazards in the category. The implications and the limitations of these studies are also discussed.
Design a combined active and passive robotic system for laser-based therapies

Port wine stain (PWS) birthmarks are congenital vascular malformations; they usually appear at birth and tend to become darker and thicker with age's growth. Vascular-targeted photodynamic therapy (PDT) is an effective approach among the treatment of PWS. However, due to the arbitrariness of manual operation and existence of pole points for laser radiation, the PWS zone was always cured unevenly in clinics. Thus, we developed a novel robotic system, which consists of passive arm and active wrist, to assist doctors in exerting PDT treatments. Passive joints are easy to interface with doctors, safe and convenience; active joints can automate the PDT, so that facilitate doctors focusing on clinical judgements and treatments rather than heavy manual work. In this paper, the mechanism of PWS and PDT were described briefly; the robotic system including the detailed robot mechanical design and kinematic analysis was discussed based on the requirements of PDT. Finally, the robot feasibility was validated by experimental results.
P2B-12 Minimum Variance Beamforming for High Frame-Rate Ultrasound Imaging

This paper investigates the application of adaptive beamforming in medical ultrasound imaging. A minimum variance (MV) approach for near-field beamforming of broadband data is proposed. The approach is implemented in the frequency domain, and it provides a set of adapted, complex apodization weights for each frequency sub-band. As opposed to the conventional, Delay and Sum (DS) beamformer, this approach is dependent on the specific data. The performance of the proposed MV beamformer is tested on simulated synthetic aperture (SA) ultrasound data, obtained using Field II. For the simulations, a 7 MHz, 128-element, phased array transducer with lambda/2-spacing was used. Data is obtained using a single element as the transmitting aperture and all 128 elements as the receiving aperture. A full SA sequence consisting of 128 emissions was simulated by sliding the active transmitting element across the array. Data for 13 point targets and a circular cyst with a radius of 5 mm were simulated. The performance of the MV beamformer is compared to DS using boxcar weights and Hanning weights, and is quantified by the Full Width at Half Maximum (FWHM) and the peak-side-lobe level (PSL). Single emission {DS Boxcar, DS Hanning, MV} provide a PSL of {-16,-36, -49} dB and a FWHM of {0.79,1.33, 0.08} mm = {3.59lambda, 6.05lambda, 0.36lambda}. Using all 128 emissions, {DS Boxcar, DS Hanning, MV} provide a PSL of {-32, -49,-65} dB, and a FWHM of {0.63, 0.97, 0.08} mm = {2.86lambda, 4.41lambda, 0.36lambda}. The contrast of the beamformed single emission responses of the circular cyst were calculated to { -18, -37, -40} dB. The simulations have shown that the frequency sub-band MV beamformer provides a significant increase in lateral resolution compared to DS, even when using considerably fewer emissions. An increase in resolution is seen when using only one single emission. Furthermore, it is seen that an increase of the number of emissions does not alter the FWHM. Thus, the MV beam- former introduces the possibility for high frame-rate imaging with increased resolution.
Novel use of pleural ultrasound can identify malignant entrapped lung prior to effusion drainage.

BACKGROUND The presence of entrapped lung changes the appropriate management of malignant pleural effusion from pleurodesis to insertion of an indwelling pleural catheter. No methods currently exist to identify entrapped lung prior to effusion drainage. Our objectives were to develop a method to identify entrapped lung using tissue movement and deformation (strain) analysis with ultrasonography and compare it to the existing technique of pleural elastance (PEL). METHODS Prior to drainage, 81 patients with suspected malignant pleural effusion underwent thoracic ultrasound using an echocardiogram machine. Images of the atelectatic lower lobe were acquired during breath hold, allowing motion and strain related to the cardiac impulse to be analyzed using motion mode (M mode) and speckle-tracking imaging, respectively. PEL was measured during effusion drainage. The gold-standard diagnosis of entrapped lung was the consensus opinion of two interventional pulmonologists according to postdrainage imaging. Participants were randomly divided into development and validation sets. RESULTS Both total movement and strain were significantly reduced in entrapped lung. Using data from the development set, the area under the receiver-operating curves for the diagnosis of entrapped lung was 0.86 (speckle tracking), 0.79 (M mode), and 0.69 (PEL). Using respective cutoffs of 6%, 1 mm, and 19 cm H2O on the validation set, the sensitivity/specificity was 71%/85% (speckle tracking), 50%/85% (M mode), and 40%/100% (PEL). CONCLUSIONS This novel ultrasound technique can identify entrapped lung prior to effusion drainage, which could allow appropriate choice of definitive management (pleurodesis vs indwelling catheter), reducing the number of interventions required to treat malignant pleural effusion.
Inhibition of Dopamine Release by Prostaglandin EP3 Receptor via Pertussis Toxin‐Sensitive and ‐Insensitive Pathways in PC12 Cells

Abstract: Prostaglandin EP3 receptor is involved in the inhibition of neurotransmitter release from presynaptic nerve terminals in various tissues. We have examined the regulation of neurotransmitter release by the EP3 receptor using a PC12 cell line that stably expresses the EP3B receptor isolated from bovine adrenal medulla. In the cells, M&B28767, an EP3 agonist, inhibited the 50 mM KCl‐ or 10 nM bradykinin‐induced [3H]dopamine release in a concentration‐dependent manner (10 pM to 0.1 µM). This inhibition was partially reversed by pretreatment with pertussis toxin, whereas under the same condition, the agonist‐induced inhibition of forskolin‐stimulated cyclic AMP accumulation was suppressed completely. In contrast, M&B28767 did not affect the high K+‐ or bradykinin‐induced increase in intracellular Ca2+ concentration. Moreover, M&B28767 also inhibited the [3H]dopamine release induced by the Ca2+ ionophore ionomycin, and this inhibition was also partially reversed by pretreatment with pertussis toxin. These results indicate that the EP3 receptor is coupled to dual pathways, pertussis toxin‐sensitive and ‐insensitive G‐protein pathways, to regulate neurotransmitter release without changing Ca2+ influx in neuronal cells.