Miracle-dz/UniFAR-FTUs huggingface dataset:
In Vitro Assembly of the Component Chains of Fibrinogen Requires Endoplasmic Reticulum Factors*

Human fibrinogen (340 kDa) is a dimer, with each identical half-molecule composed of three different polypeptides (Aα, 66 kDa; Bβ, 55 kDa; and γ, 48 kDa). To understand the mechanisms of chain assembly, a coupled in vitro transcription translation system capable of assembling fibrinogen chains was developed. Fibrinogen chain assembly was assayed in an expression system coupled to rabbit reticulocyte lysate in the presence or absence of dog pancreas microsomal membranes. Fibrinogen chain assembly required microsomal membranes and oxidized glutathione. Co-expression of two of the chains, Bβ and γ or Aα and γ, yielded free chains and two-chain complexes. Unlike combinations of Aα with γ and Bβ with γ, co-expression of Aα and Bβ did not form a single two-chain complex but produced a mixture of two-chain complexes. Co-expression of all three chains yielded free chains, two-chain complexes, and higher molecular weight complexes that corresponded to a half-molecule and to fully formed fibrinogen. Upon treatment of this mixture with thrombin and factor XIIIa, a γ·γ dimer, similar to that obtained from cross-linked human fibrin, was produced, indicating that properly folded fibrinogen was formed in vitro. Molecular chaperones may participate in fibrinogen assembly, since antibodies to resident proteins of the endoplasmic reticulum (BiP, Hsp90, protein disulfide isomerase, and calnexin) co-precipitated the chaperones together with nascent fibrinogen chains and complexes.
Calibration of Equivalent Finite Element Model for Packaged Printed Circuit Board by Experimental Modal Analysis

In this work, the experimental modal analysis (EMA) was performed to establish an equivalent finite element (FE) model for a standard JEDEC drop test printed circuit board (PCB) mounted with packages in a full array. Material properties of the equivalent FE model of the packaged PCB were calibrated through an optimization process with respect to natural frequencies based on EMA results obtained with a free boundary condition. The model was then applied to determine screwing tightness of the packaged PCB corresponding to a fixed boundary condition with the four corners of the PCB constrained, as defined by JEDEC for a board-level drop test.
The Effects of Koji and Histidine on the Formation of Histamine in Anchovy Sauce and the Growth Inhibition of Histamine Degrading Bacteria with Preservatives

Koji and histidine have been applied to accelerate the fermentation of fish sauce for the purpose of shortening the manufacturing period and cost. The histamine contents of anchovy sauces fermented at 15 and 25 °C for 12 months were 3.7-3.8 mg/mL. The histamine content increased up to 6 months of ripening and then decreased, where at 25 °C was higher than at 15 °C at 6 months of ripening, but similar at 12 months. Koji decreased the histamine formation at 3 months at 15 °C, but no significant difference at 12 months, whereas histidine increased at 6 months at 15 °C, but no great difference at 25 °C. Histamine degrading bacteria isolated from the anchovy was identified to Staphylococcus xylosus. Optimal pH for the growth of S. xylosus was neutral. The growth of S. xylosus was strongly inhibited as the concentration of NaCl, benzoic acid, and sorbic acid increased.
Quantitative analysis of nonequilibrium, denaturant-dependent protein folding transitions.

The free energy of folding of small, one-domain proteins is generally measured with denaturant-induced unfolding/refolding equilibria based on the two-state model of protein folding. There is however an increasing number of reports on proteins that do not attain their folding equilibrium and show, even after long-term incubation, unfolding transitions at high and refolding transitions at low denaturant concentrations. We present a theory for the quantitative description of this nonequilibrium behavior in protein folding, which is exclusively based on the two-state assumption and the exponential dependence of the rate constants of unfolding and refolding on denaturant concentration. Using a hyperstable variant of a pilin domain from E. coli type 1 pili that does not reach its folding equilibrium within several years, we demonstrate that the method provides reliable information on the free energy of folding, the solvent accessibility of the transition state of folding relative to the native and unfolded sta.
Standardizing Biochemistry Dataset for Medical Research

Harnessing clinical datasets from the repository of electronic health records for research and medical intelligence has become the norm of the 21st century. Clinical datasets present a great opportunity for medical researchers and data analysts to perform cohort selections and data linkages to support better informed clinical decision-making and evidence-based medicine. This paper utilized Logical Observation Identifiers Names and Codes (LOINC®) encoding methodology to encode the biochemistry tests in the anonymized biochemistry dataset obtained from the Health Informatics Centre (HIC) at the University of Dundee. Preliminary results indicated that the encoded dataset was flexible in supporting statistical analysis and data mining techniques. Moreover, the results indicated that the LOINC codes cover most of the biochemistry tests used in National Health Service (NHS) Tayside, Scotland.
The modified Gait Abnormality Rating Scale for recognizing the risk of recurrent falls in community-dwelling elderly adults.

BACKGROUND AND PURPOSE The purpose of this study was to determine the reliability and validity of measurements obtained with a seven-item modified version of the Gait Abnormality Rating Scale (GARS-M), an assessment of gait designed to predict risk of falling among community-dwelling, frail older persons. SUBJECTS Fifty-two community-dwelling, frail older persons, with a mean age of 74.8 years (SD = 6.75), participated. METHODS A history of falls was determined from self-report or by proxy report. The GARS-M was scored from videotapes of subjects walking at self-selected paces. Gait characteristics were recorded during a timed walk on a 6-m brown-paper walkway. RESULTS Scores obtained by three raters for 23 subjects demonstrated moderate to substantial intrarater and interrater reliability. Concurrent validity, as assessed by Spearman rank-order correlation coefficients, was demonstrated for the relationship between GARS-M scores and stride length (r = -.754) and for the relationship between GARS-M scores and walking speed (r = -.679). Mean GARS-M scores distinguished between frail older persons with and without a history of recurrent falls (mean GARS-M scores of 9.0 and 3.8, respectively). CONCLUSION AND DISCUSSION The GARS-M is a reliable and valid measure for documenting gait features associated with an increased risk of falling among community-dwelling, frail older persons and may provide a clinically useful alternative to established quantitative gait-assessment methods.
Ethylene and the regulation of senescence processes in transgenic Nicotiana sylvestris plants.

BACKGROUND AND AIMS Exposure of plants to ethylene can influence a spectrum of developmental processes including organ senescence and abscission. The aim of this study was to examine the role of the gaseous regulator in Nicotiana sylvestris plants exhibiting a silenced or constitutive ethylene response. METHODS Transgenic N. sylvestris plants were generated that either ectopically expressed the Arabidopsis mutant ethylene receptor ETR1-1 or the tomato EIN3-like (LeEIL1) gene. Highly expressing homozygous lines were selected and the time-course of development, from germination to organ senescence, was studied. KEY RESULTS Fifty percent of the homozygous Pro(35S):ETR1-1 lines examined showed a high susceptibility to collapse prior to flowering, with plant death occurring within a few days of leaf wilting. The time-course of leaf senescence in the remaining Pro(35S):ETR1-1 lines was visibly arrested compared to wild type (negative segregant) plants and this observation was reaffirmed by chlorophyll and protein analysis. Petal necrosis was also delayed in Pro(35S):ETR1-1 lines and corolla abscission did not take place. When senescence of Pro(35S):ETR1-1 plants did take place this was accompanied by leaf bleaching, but tissues remained fully turgid and showed no signs of collapse. A single Pro(35S):LeEIL1 line was found to exhibit consistently accelerated leaf and flower senescence and precocious flower bud shedding. CONCLUSIONS These observations support a role for ethylene in regulating a spectrum of developmental events associated with organ senescence and tissue necrosis. Furthermore, the transgenic lines generated during this study may provide a valuable resource for exploring how senescence processes are regulated in plants.
Laboratory Studies of Psychological Stress

This paper reviews current methods of studying psychological stress in contrived settings. Stress may be operationally defined in terms of stimuli, responses or both. On the stimulus side, types of stressors are classified on the basis of stimulus characteristics or kinds of threat involved. On the response side, various indicators of stress are discussed including measures of changes in affective state, adaptive functioning and physiological arousal.
Whole blood amino acid changes following respiratory-acquired Pasteurella tularensis infection in man.

Abstract : Significant changes in the whole blood amino acids of volunteers with respiratory-acquired Pasteurella tularensis (SCHU-S4) infection have been described. A decrease in whole blood amino acids occurred 12 to 60 hours after exposure and prior to the time that clinical symptomatology was apparent. The factors responsible for this decreased amino acid concentration were discussed but remain undefined. These changes were specifically related to infection, in that sham-exposed and similarly treated subjects did not manifest a similar response. The pattern of response was such that, in retrospect, it appeared to have predictive value for determining which subjects had been infected.
Antibiotic resistance of heterotrophic bacteria in Shira lake: natural and anthropogenic impacts

Studies were conducted to investigate the antibiotic resistance of freshwater, halophile and moderate halotolerant bacteria isolated from the nearshore part of Shira lake, which is affected by the activity of a health resort, and from the central part of the lake in the summer (June to August) of 1999. It has been shown that the allochthonous microflora, which is brought into the lake with the resort effluent in mid-summer, is the anthropogenic factor contributing to an increase in the number of freshwater bacteria that feature multiple antibiotic resistance in the central part of the lake. It has been found that resistance to ampicillin of freshwater and halophile heterotrophic bacteria is related to the increase in the biomass of blue-green and green algae in the central part of Shira lake between mid-July and the end of August. A mathematical model has been constructed to describe the dynam- ics of the antibiotic resistance of heterotrophic bacteria in the close-to-resort and the central parts of Shira lake under natural and anthropogenic impacts.
Hormonal Levels in Fox‐Fordyce disease

It is now 70 years since this condition was first described (Fox & Fordyce, 1902). Hormonal factors have been implicated in the aetiology (Shelley & Levy, 1956; Montes et al., 1961) and when a case of the disorder was seen recently the opportunity was taken to investigate the endocrine background by means of recent techniques (Mayes & Nugent, 1968; Norymberski, 1952; Horning et al., 1963; Kirschner & Lipsett, 1963; Cox, 1959).
Time constant spectra based fitting of thermal model parameters

This paper discusses the problem offitting parameters of a detailed circuit thermal model when the complete data on problem geometry or material properties are not available. The problem is illustrated based on a practical example of a test hybrid circuit. Unknown model parameter values are determined here from dynamic temperature measurement results which were processed employing the Network Identification by Deconvolution method. The thermal model is solved using the numerical finite element method and the analytical Green's function method. Then, the simulated heating curves are compared with the measured ones.
The Amud 7 skeleton--still a burial. Response to Gargett.

Commentaire d'un article ecrit par Gargett (Journal of Human evolution 1999) dans lequel l'auteur penche pour une inhumation naturelle d'un enfant dans la grotte Amud. L'auteur de ce present article, n'est pas d'accord avec Gargett et prefere,l'hypothese d'une inhumation volontaire.
Especificación formal de Protocolos Criptográficos en Cálculo de Situaciones

Resumen: Presentamos un modelo generico en Calculo de Situaciones (C.S.) para formalizar protocolos criptograficos. Se distingue de otras logicas para la modelizacion criptografica que usan los conceptos de creencia o secreto porque nuestra propuesta esta basada en el concepto de conocimiento y no es necesaria ninguna construccion adicional fuera del C.S. Como muestra, presentamos la especificacion formal del protocolo de autentificacion del ISO/IEC 9798-2 y se ilustra como demostrar diferentes propiedades de correccion sobre el.
Immersion and invariance control for lateral dynamics of autonomous vehicles, with experimental validation

Autonomous intelligent vehicles are under intensive development, especially this last decade. The autonomous navigation consists on developing three main key steps: 1. environment perception, 2. path planning and decision, and 3. vehicle control. This paper focus on the lateral control of intelligent vehicles ; it presents design and experimental validation of a vehicle lateral controller based on Immersion and Invariance (I&I) principle, to minimize the lateral displacement of the autonomous vehicle with respect to a given reference trajectory. The control input is the steering angle and the output is the lateral error displacement. The closed-loop system simulated on Matlab-Simulink has been compared to the experimental data acquired on our vehicle DYNA, a Peugeot 308, according to several driving scenarios. The experimental validation shows robustness and good performance of the proposed control approach.
DTMB-GPS software receiver for urban and indoor positioning

This paper presents realization of positioning based on DTMB (Digital Television Terrestrial Multimedia Broadcasting) and GPS (Global Positioning System) in urban or indoor environment by means of a Software Defined Radio (SDR). DTMB signal's unique feature in super-frame is analyzed and a new correlation method without generating local PN sequence is proposed. Double delta correlator and Kalman filter are used in multipath mitigation, solution smoothing and position prediction. Real scenario tests have been performed to verify the validation of the hybrid positioning system combining DTMB and GPS, showing fast and reliable performance under GPS limited conditions.
NUMERICAL CALCULUS OF RESONANT FREQUENCY CHANGE BY 3 D RECONSTRUCTION OF THERMAL DEFORMED ACCELERATOR TUBE

Thermal deformation caused by Non-uniform temperature distribution in disk-loaded waveguide affects the resonant frequency of LINAC deeply. A new approach of multi-disciplinary integration is adopted to study the influence of the thermal deformation on the resonant frequency. The kernel technology is to simulate the thermal deformation of the accelerator tube loaded RF power via thermal-structure coupling analysis in FEM software I-DEAS first. Then a reconstruction of a new 3D geometric model is done from the nodes of deformed finite element model of accelerating cavities with self customized program. Finally the new model is coupled into the Microwave Studio to recalculate the resonant frequency. The method can simulate the real resonant frequency change cased by non-uniform deformation with high precision.
RASONs: a novel antisense oligonucleotide therapeutic approach for asthma

Inhalation based approaches enable the local delivery of antisense oligonucleotides (ASONs) to the respiratory tract and thus facilitate the ability of ASONs to target and modulate the activity of discordantly expressed respiratory disease genes. Studies involving EPI-2010, a respirable antisense oligonucleotide (RASON), targeting the adenosine A1 receptor, a G-protein-coupled-receptor (GPCR) that plays an important role in the aetiology of asthma, demonstrate that ASON therapeutics can be delivered directly to the lung as an aerosol. EPI-2010 has been shown to inhibit adenosine A1 receptor expression and significantly improve allergen-induced airway obstruction and bronchial hyper-responsiveness in animal models of human asthma. Absorption, tissue distribution, metabolism and excretion (ADME) and safety studies of aerosolised EPI-2010 suggest that phosphorothioate RASONs can be delivered to target respiratory tissues in low, safe, efficacious and long-acting doses. This supports the concept that RASONs offer the potential to address a variety of respiratory targets including those for which approaches employing systemic distribution and systemic bioavailability of the therapeutic agent may be undesirable. In addition, our studies with EPI-2010 indicate that the RASON approach may represent a technology that is uniquely positioned to address the challenges of the post-genome era in respiratory drug discovery, since it enables simultaneous in vivo target validation and antisense therapeutic discovery in an accelerated timeframe.
Oncology Drug Discovery Applications Using the FMAT™ 8100 HTS System

High-throughput screening (HTS) for potential anticancer agents requires a broad portfolio of assay platforms that may include kinase enzyme assays, protein-protein binding assays, and functional cell-based apoptosis assays. The authors have explored the use of fluorometric microvolume assay technology (the FMAT™ 8100 HTS System) in three distinct homogeneous HTS assays: (1) a Src tyrosine kinase enzyme assay, (2) a Grb2-SH2 protein-peptide interaction assay, and (3) an annexin V binding apoptosis assay. Data obtained from all three assays suggest that the FMAT system should facilitate the implementation of homogeneous assays for a wide variety of molecular targeted and cell-based screens. (Journal of Biomolecular Screening 2003:81-88)
Model Checking Probabilistic Timed Automata with One or Two Clocks

Probabilistic timed automata are an extension of timed automata with discrete probability distributions.We consider model-checking algorithms for the subclasses of probabilistic timed automata which have one or two clocks. Firstly, we show that PCTL probabilistic model-checking problems (such as determining whether a set of target states can be reached with probability at least 0.99 regardless of how nondeterminism is resolved) are PTIME-complete for one clock probabilistic timed automata, and are EXPTIME-complete for probabilistic timed automata with two clocks. Secondly, we show that the model-checking problem for the probabilistic timed temporal logic PTCTL is EXPTIME-complete for one clock probabilistic timed automata. However, the corresponding model-checking problem for the subclass of PTCTL which does not permit both (1) punctual timing bounds, which require the occurrence of an event at an exact time point, and (2) comparisons with probability bounds other than 0 or 1, is PTIME-complete.
Human Visual System Based Framework For Gender Recognition

A face reveals a great deal of information to a perceiver including gender. Humans use specific information (cue) from a face to recognize gender. The focus of this paper is to find out this cue when the Human Visual System (HVS) decodes gender of a face. The result can be used by a Computer Vision community to develop HVS inspired framework for gender recognition. We carried out a Pyscho-visual experiment to find which face region is most correlated with gender. Eye movements of 15 observers were recorded using an eye tracker when they performed gender recognition task under controlled and free viewing condition. Analysis of the eye movement shows that the eye region is the most correlated with gender recognition. We also proposed a HVS inspired automatic gender recognition framework based on the Psycho-visual experiment. The proposed framework is tested on FERET database and is shown to achieve a high recognition accuracy.
Model-based testing of dynamic component systems

This dissertation devotes to the question whether the established technique of model based testing (MBT) can be applied to a special type of software component systems called dynamic component systems (DCSs). DCSs have the special characteristic that they support the change of component instance compositions during runtime of the system. In these systems, each component instance exhibits an own lifecycle. This makes it possible to update existing, or add new components to the system, while it is running. Such changes cause that functionality provided by the component instances may become restricted or unavailable at any time. This characteristic of DCSs makes the development of components difficult because required and used functionality is not available all the time. The goal of this dissertation is to develop a systematic testing approach which allows to test a component’s tolerance to dynamic availability during development time. We analyze, to what extend existing MBT approaches can be reused or adapted. In MBT, test cases are generated using formal structural and behavioral models, the so called test models, and heuristic test selection methods. To enable the application of MBT to DCSs, we first formalize the artifacts and processes of MBT. This formalization uses model-based technologies and abstracts some properties of the system under test (SUT) away. Next, we refine this formalization for reactive, event-based, potentially asynchronous communicating and timeless component systems. For the test models, we use the Unified Modeling Language (UML), in special, UML class diagrams and UML Statecharts. For test generation we use an model checking approach. The last step to allow application of MBT to DCSs is achieved by a special structure for the test modes, where the lifecycle of dynamic components is imitated. With this approach, it is possible to generate test cases which stimulate the SUT to bring component instances to desired phases of their lifecycle, and therefore reach dynamic availability in the system. For the question of the dissertation, we conclude that the application of MBT for DCSs does not need any refinement for the used modeling languages for creating
DRA-array with 75% reduction in elements number

The gain of dielectric resonator antennas (DRA) above a ground plane is enhanced by placing a dielectric disc on top of the ground plane by a half wavelength. The dielectric disc is of a quarter dielectric wavelength thicknesses. This technique is also used to enhance the array antenna gain as well as reducing the number of the array elements by 75%. The dielectric slab acts as a lens as well as a Radom. A microstrip feeding network is used with all 50 Ohms microstrip lines with the 2 D arrays that reduce the feeding network discontinuities.