Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular characterization and the effectiveness of native entomopathogenic Beauveria bassiana strains against adults of Mediterranean fruit fly (Ceratitis capitata)

Molecular characterization of locally isolated Beauveria bassiana (Cordycipitaceae, Hypocreales) strains and their pathogenicity against Ceratitis capitata (Diptera: Tephritidae) adults are herein reported for the first time. Molecular identification based on the phylogenetic analyses of ITS-rDNA and combined data of TEF1 and Bloc, revealed that all fungal strains were conclusively assigned to B. bassiana clade. Interestingly, Maximum parsimony based-phylogeny analysis of the combined TEF1 and Bloc sequences detected additional genotypes among the sub-clades than ITS-rDNA analysis. Genetic diversity among strains was performed using random amplified polymorphic DNA (RAPD) markers. RAPD genotyping segregated all the strains into three clusters and characterized the 15 strains with different genotypes. RAPD-PCR clustering analysis showed that B. bassiana strains were relatively separated according to geographical origin. Molecular analyses using nuclear genes (TEF1 and Bloc) and RAPD markers suggest that B. bassiana is an aggregate of species, rather than a single species. Under laboratory conditions, B. bassiana strains were highly pathogenic to C. capitata adults with mortality rate in the range of 71.67–91.67%. Based on the Average of Survival Time (AST) and median lethal times (LT50) values, fungal strains were able to reduce significantly lifespan of flies compared to controls. Further experiments under field conditions are required to evaluate the real contribution of these fungal strains as promising biological control agents against C. capitata adults in Morocco.
ACC/AHA 2002 guideline update for exercise testing: summary article: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Committee to Update the 1997 Exercise Testing Guidelines).

These guidelines have been reviewed over the course of the past 5 years since their publication in the Journal of the American College of Cardiology, (Full Text—J Am Coll Cardiol 1997;30:260-315; Executive Summary—Circulation 1997;96:345-54). This update is based on the most significant advances in exercise testing that have been published during that time frame. These guidelines are available on the Web sites of both the American College of Cardiology and the American Heart Association. Deleted text is indicated by strikeout, and revised text is underlined.
Conductance Quantization at a Half-Integer Plateau in a Symmetric GaAs Quantum Wire

We present data from an induced gallium arsenide (GaAs) quantum wire that exhibits an additional conductance plateau at 0.5(2e2/h), where e is the charge of an electron and h is Planck's constant, in zero magnetic field. The plateau was most pronounced when the potential landscape was tuned to be symmetric by using low-temperature scanning-probe techniques. Source-drain energy spectroscopy and temperature response support the hypothesis that the origin of the plateau is the spontaneous spin-polarization of the transport electrons: a ferromagnetic phase. Such devices may have applications in the field of spintronics to either generate or detect a spin-polarized current without the complications associated with external magnetic fields or magnetic materials.
Experimental Observations of Construction Methods for Double Array Structures Using Linear Functions

A trie is an ordered tree data structure to store keywords. It is used in natural language processing and so on. The trie is represented by the double array. The double array can retrieve fast at time complexity of O(1). The double array using linear functions (DALF) is proposed as a compression method of the double array. DALF reduces space usage of the double array to 60%. DALF is built by using parameters, and its space usage and its construction time depend on these parameters. However, appropriate values of them are not determined. This paper observes these parameters and evaluates parameters by experiments. From experiments, appropriate parameters are found, and it turns out that DALF can be built more efficiently by keyword sets including multibyte characters.
A novel recommender algorithm using information entropy and secondary-clustering

The accuracy of final recommender results is always affected by less active evaluating information of users. We present a secondary-clustering recommender algorithm based on information entropy in this paper. By extracting characteristic words and corresponding weights, we compute the information entropy value of each webpage text browsed by users. Moreover, we obtain the content of recommender results by twice text clustering and combine the continuous random variable of uniform distribution with the approaches of the average entropy value approximation and the logarithmic function fitting, etc. The experimental results show that the new algorithm is stable during the real system operation and improves the accuracy of final recommender results comparing to the traditional algorithms.
Nucleotide sequence of HSUR 6 and HSUR 7, two small RNAs of herpesvirus saimiri.

Herpesvirus saimiri is a common virus of the squirrel monkey (Saimiri scuireus); it induces fatal lymphoma and leukemia in various other species of New World primates and transforms human and monkey T-lymphocytes to continuous growth in cell culture (1). The leftmost 5.5 kbp of genomic L-DNA have been shown to encode a set of five small RNAs, the first viral U-RNAs (HSURs) described to date (2-4). They are related to each other, but different from small RNAs of other viruses, and they are distinct from any previously characterized cellular U RNAs. We determined the nucleotide sequence of the region adjacent to that encoding HSUR 1-5 and found two additional genes for viral U RNAs, HSUR 6 and HSUR 7. The genes have characteristic, U RNA specific transcription control regions including enhancer octamer motifs, snRNA TATA boxes, and 3'-end formation signals, which are comparable to those found in cellular U RNA genes. Total RNA from the marmoset T cell line 1670, which had been transformed by herpesvirus saimiri, was analysed by primer extension experiments for expression of predicted U RNAs. The conserved 3'-end regions of the transcripts have been used to design oligonucleotides as primers specific for HSUR 6 and HSUR 7 (Figure 2). Complementary DNA molecules synthesized were found to be 81 nucleotides (nt) and 75 nt, respectively, in length (Figure 1). This is in agreement with the expected size derived from sequence analysis. Computer assisted searches did not reveal any other HSUR sequences within the genome of herpesvirus saimiri which has been completely sequenced recently.
Impact of prolonged sitting on vascular function in young girls

What is the central question of this study? Children are spending more than 60% of their waking day sedentary. The consequences of excessive sedentary behaviour are not well understood in the child, but there is growing evidence that with increasing sedentary time, cardiovascular risk in childhood also increases. What is the main finding and its importance? Our findings show that a 3 h period of uninterrupted sitting causes a profound (33%) reduction in vascular function in young girls. Importantly, we also demonstrate that breaking up sitting with regular exercise breaks can prevent this.
Localization of PcsB of Streptococcus pneumoniae and Its Differential Expression in Response to Stress

ABSTRACT PcsB of Streptococcus pneumoniae is an essential hydrolase involved in the separation of dividing cells. In this study, it was found that PcsB localizes to the plasma membrane and is released into the growth environment, yet it is detectable on the pneumococcal surface by flow cytometry analysis. High temperature and osmolarity led to upregulation of pcsB expression.
Urinary Markers of Kidney Injury and Kidney Function Decline in HIV-Infected Women

Objective:HIV-infected persons have substantially higher risk of kidney failure than persons without HIV, but serum creatinine levels are insensitive for detecting declining kidney function. We hypothesized that urine markers of kidney injury would be associated with declining kidney function among HIV-infected women. Methods:In the Women's Interagency HIV Study, we measured concentrations of albumin-to-creatinine ratio, interleukin-18 (IL-18), kidney injury marker-1 (KIM-1), and neutrophil gelatinase-associated lipocalin from stored urine among 908 HIV-infected and 289 HIV-uninfected participants. Primary analyses used cystatin C-based estimated glomerular filtration rate (CKD-EPI eGFRcys) as the outcome, measured at baseline and 2 follow-up visits over 8 years; secondary analyses used creatinine (CKD-EPI eGFRcr). Each urine biomarker was categorized into tertiles, and kidney decline was modeled with both continuous and dichotomized outcomes. Results:Compared with the lowest tertiles, the highest tertiles of albumin-to-creatinine ratio (−0.15 mL/min per 1.73 m2, P < 0.0001), IL-18 (−0.09 mL/min per 1.73 m2, P < 0.0001) and KIM-1 (−0.06 mL/min per 1.73 m2, P < 0.001) were independently associated with faster eGFRcys decline after multivariate adjustment including all 3 biomarkers among HIV-infected women. Among these biomarkers, only IL-18 was associated with each dichotomized eGFRcys outcome: ≥3% (relative risk = 1.40; 95% confidence interval: 1.04 to 1.89); ≥5% (1.88; 1.30 to 2.71); and ≥10% (2.16; 1.20 to 3.88) for the highest versus lowest tertile. In alternative models using eGFRcr, the high tertile of KIM-1 had independent associations with 5% (1.71; 1.25 to 2.33) and 10% (1.78; 1.07 to 2.96) decline, and the high IL-18 tertile with 10% decline (1.97; 1.00 to 3.87). Conclusions:Among HIV-infected women in the Women's Interagency HIV Study cohort, novel urine markers of kidney injury detect risk for subsequent declines in kidney function.
Towards an Integrated Conceptual Model for Cloud Adoption in Saudi Arabia

There are several advantages of utilising cloud computing in organisations such as cost saving and flexibility in acquiring resources. The use of cloud computing in developing countries, such as Saudi Arabia, is still in its early stages and has not been as widely adopted there as in developed countries. In fact, moving a current system to the cloud depends on many factors that may affect a Saudi Arabian organisation's decision to adopt the cloud. In order to encourage the adoption of cloud technology it is essential to understand why some enterprises are more prepared than others to move to the cloud. Hence, the aim of this research is to examine factors that might impact on a Saudi Arabian organisation's intention to adopt cloud computing. In this paper, we propose a conceptual model which integrates aspects of the Technology Organisation Environment (TOE) framework. The proposed model identifies the key factors that might influence organisations to employ cloud services. Our findings show that all the proposed factors in the cloud adoption model, except for competitive pressure and trading partner pressure, are statically significant.
Differential detergent fractionation of isolated hepatocytes: Biochemical, immunochemical and two‐dimensional gel electrophoresis characterization of cytoskeletal and noncytoskeletal compartments

Two‐dimensional (2‐D) gel electrophoresis is often used in toxicologic and metabolic studies to assess treatment‐ or stage‐specific changes in protein synthesis, degradation or posttranslational modification. When combined with cell fractionation studies the detectability of low abundance proteins is enhanced, and changes in subcellular distribution of proteins can also be monitored. Detergent fractionation is a simpler alternative to differential pelleting, which partitions cellular constituents into functionally distinct populations while preserving cytoskeletal integrity. We defined and characterized a differential detergent fractionation (DDF) protocol to enable protein dynamics in cytoskeletal and noncytoskeletal compartments of isolated hepatocytes to be monitored simultaneously. Rat hepatocytes were maintained in suspension culture and fractionated by sequential extraction with detergentcontaining buffers (digitonin/EDTA, Triton/EDTA, Tween/deoxycholate). DDF reproducibly yielded four electrophoretically distinct fractions enriched in cytosolic, membrane‐organelle, nuclear membrane and cytoskeletal‐matrix markers, respectively. Immunoblotting with over 20 different antibodies corroborated the selectivity of fractionation and was used to characterize the distribution profiles of cytoskeletal (actin, tubulins, cytokeratins, vinculin, myosin, desmoplakins, fodrin, nuclear lamins) and noncytoskeletal proteins (heat‐shock 70 proteins, glutathione‐S‐transferase, calpains, carbamoyl phosphate synthetase, etc.), as well as to identify spots in 2‐D gels. Detergent buffers were compatible with equilibrium or nonequilibrium 2‐D gel electrophoretic analysis. Extensive 2‐D maps of acidic and basic proteins in each fraction were generated along with a tabular listing of Mr and pI. Thus, DDF reproducibly partitions hepatocytic proteins into functionally distinct cytoskeletal and noncytoskeletal compartments that are readily analyzed by 2‐D gel electrophoresis. DDF is simple, applicable to use with other cell types or culture systems and is especially useful when biomaterial is limited (i.e., clinical studies).
Modeling of carbon nanotube interconnects and comparative analysis with Cu interconnects

Metallic carbon nanotube (CNT) interconnect is regarded as a competitive candidate for next generation of interconnect. An equivalent transmission line model for CNT is proposed in this paper. In our model, the fundamental contact resistance (quantum resistance) is regarded as lumped while the scattering resistance is distributed. The performance of CNT interconnect is examined and compared with the Cu interconnect. It is found that CNT interconnects do not out-perform Cu wire at local interconnect level. However, they have better performance than Cu interconnect at intermediate and global interconnect level. With the technology advancing, their advantages could become greater.
Biodiversity and Emerging Biogeography of the Neutrophilic Iron-Oxidizing Zetaproteobacteria

ABSTRACT Members of the neutrophilic iron-oxidizing candidate class Zetaproteobacteria have predominantly been found at sites of microbially mediated iron oxidation in marine environments around the Pacific Ocean. Eighty-four full-length (>1,400-bp) and 48 partial-length Zetaproteobacteria small-subunit (SSU) rRNA gene sequences from five novel clone libraries, one novel Zetaproteobacteria isolate, and the GenBank database were analyzed to assess the biodiversity of this burgeoning class of the Proteobacteria and to investigate its biogeography between three major sampling regions in the Pacific Ocean: Loihi Seamount, the Southern Mariana Trough, and the Tonga Arc. Sequences were grouped into operational taxonomic units (OTUs) on the basis of a 97% minimum similarity. Of the 28 OTUs detected, 13 were found to be endemic to one of the three main sampling regions and 2 were ubiquitous throughout the Pacific Ocean. Additionally, two deeply rooted OTUs that potentially dominate communities of iron oxidizers originating in the deep subsurface were identified. Spatial autocorrelation analysis and analysis of molecular variance (AMOVA) showed that geographic distance played a significant role in the distribution of Zetaproteobacteria biodiversity, whereas environmental parameters, such as temperature, pH, or total Fe concentration, did not have a significant effect. These results, detected using the coarse resolution of the SSU rRNA gene, indicate that the Zetaproteobacteria have a strong biogeographic signal.
[Poster] Interactive deformation of real objects

This paper presents a method for interactive deformation of a real object. Our method uses a predefined 3D model of a target object for tracking and deformation. A camera pose relative to the target object is estimated using 3D model-based tracking. Object region in camera image is obtained by projecting the 3D model onto image plane with the estimated camera pose, and a texture map is extracted from the object region and mapped to the 3D model. Then a texture-mapped model is rendered based on a mesh deformed by user via Laplacian operation. Experimental results demonstrate that our method provides user interactions with 3D real objects on real scenes, not augmented virtual contents.
Low Power Low Jitter 0 . 18 CMOS Ring VCO Design with Strategy based on EKV 3 . 0 Model

In this paper, the design of micro-power CMOS ring VCO with minimum jitter intended for a concept of frequency synthesizer in biotelemetry systems is studied. A design procedure implemented in MATLAB is described for a circuit realization with TSMC 0. 18μm CMOS technology. This conventional design methodology based on EKV3.0 model is clearly suited to the challenges of analog circuits design with reduced channel width. Measures realized with ADS confirmed methodology capability to circuit sizing respecting the specifications of application. The designed ring VCO operates at a central frequency of 433MHz in ISM band with an amplitude of oscillation equal to 500 mV. The integration area was intrinsic (without buffers and without external capacitances). The simulated phase noise is about -108 dBc/Hz at 1MHz, the value of rms jitter is 44.8 ps and the power consumption of the designed VCO is 6.37 mW @ 433 MHz. Keywords—Ring VCO; jitter; power consumption; EKV model; MATLAB
Controlled 3D Biped Stepping Animations Using the Inverted Pendulum and Impulse Constraints

This paper presents a method for generating intelligent upright biped stepping motions for real-time dynamic environments. Our approach extends the inverted pendulum (IP) model by means of an impulse-based technique to achieve rigid-leg constraints during foot support transitions. The impulse-based method in cooperation with the IP method provides a computationally fast, straightforward, and robust solution for achieving stiff-knee joints that are desired during casual stepping motions, such as standing and walking. Furthermore, we demonstrate how the impulse-based inverted pendulum (IIP) model can be extended to embody rotational information to synthesize more dynamic actions, such as when the feet leave the ground or when slipping (i. e., foot friction).
Development of a new academic digital library: a study of usage data of a core medical electronic journal collection.

OBJECTIVES The current study evaluates the results of a previously reported method for creating a core medical electronic journal collection for a new medical school library, validates the core collection created specifically to meet the needs of the new school, and identifies strategies for making cost-effective e-journal selection decisions. METHODS Usage data were extracted for four e-journal packages (Blackwell-Synergy, Cell Press, Lippincott Williams & Wilkins, and ScienceDirect). Usage was correlated with weighted point values assigned to a core list of journal titles, and each package was evaluated for relevancy and cost-effectiveness to the Florida State University College of Medicine (FSU COM) population. RESULTS The results indicated that the development of the core list was a valid method for creating a new twenty-first century, community-based medical school library. Thirty-seven journals are identified for addition to the FSU COM core list based on use by the COM, and areas of overlapping research interests between the university and the COM are identified based on use of specific journals by each population. CONCLUSIONS The collection development approach that evolved at the FSU COM library was useful during the initial stages of identifying and evaluating journal selections and in assessing the relative value of a particular journal package for the FSU COM after the school was established.
RFID For Document Management: Assessment of Scenarios Implementation: An Arab case study

Radio Frequency Identification (RFID) systems are used in a variety of applications to uniquely identify physical objects. The operation of RFID systems often involves a situation in which numerous tags are present in the interrogation zone of a single reader at the same time. Although RFID is currently a hot topic, many organizations are slow in adopting RFID to conduct more effective and efficient business processes. This study presents an analysis toward understanding the evaluation of business value and introducing RFID at a public ministry in an Arab country. This study propose a framework based of three phases for the assessment of RFID technology, hoping that a better understanding of the business value of RFID will encourage more organizations to implement it.
Monolithic NMOS digital integrated circuits in 6H-SiC

We report the first digital monolithic integrated circuits in the wide bandgap semiconductor silicon carbide (SiC). These logic gates are implemented in enhancement-mode NMOS using ion implanted MOSFET's with non-self-aligned metal gates. We have fabricated and characterized inverters, NAND and NOR gates, XNOR gates, D-latches, RS flip-flops, binary counters, and half adders. All circuits operate properly from room temperature to over 300/spl deg/C. <<ETX>>
Household transmission of Streptococcus pneumoniae, Alberta, Canada.

Proven or presumptive multidrug-resistant Streptococcus pneumoniae pneumonia was diagnosed simultaneously in three married couples in Alberta, Canada. The pair of isolates from each couple had identical antibiotic resistance profiles, serotypes, and pulsed-field gel electrophoresis patterns. One or more of these cases could have been prevented by S. pneumoniae vaccine.
Bacterial infection of wounds: fibronectin-mediated adherence group A and C streptococci to fibrin thrombi in vitro

Adherence of group A, B, and C streptococci to fibrin thrombi was studied by using a novel fluorochrome microassay carried out in microdilution plates in which fibrin thrombi had been prepared by clotting citrated human, cattle, or horse plasma. Substantial adherence was observed with various strains of group A and C streptococci, whereas little was observed with group B streptococci. Adherence of all group A and C streptococcal strains decreased by up to 40% when fibronectin was depleted from the plasmas used for preparing fibrin thrombi, and fibronectin repletion increased adherence of streptococci in a dose-dependent manner. Addition of the 210-kilodalton C-terminal fragment of fibronectin to fibronectin-depleted plasma restored the adherence of group C but not group A streptococci, whereas addition of the 29-kilodalton N-terminal fragment was without any effect for all tested streptococcal strains. Prior incubation of group A and C streptococcal strains with fibronectin markedly increased their adherence, but treatment with proteases abolished their ability to adhere to fibrin thrombi. Adherence of group B streptococci was not affected by either fibronectin depletion or proteolytic digestion. These results indicate that both fibronectin incorporated into the fibrin matrix of thrombi and soluble fibronectin can mediate adherence of group A and C streptococci to fibrin thrombi and that binding sites for fibronectin located on the bacterial surface mediate this adherence.