Miracle-dz/UniFAR-FTUs huggingface dataset:
History and Diversity: Explorations at the Intersection of Ecology and Evolution

Phylogenetic analysis provides an important tool for assessing the influence of historical and evolutionary processes on the structure of contemporary ecological systems. Patterns of diversity, for example, represent the regional buildup of species through immigration and diversification, their loss through extinction, and the sorting of species ecologically within the region. Colonization‐extinction dynamics on islands can be inferred from lineage accumulation through time. Lineage branching within clades can be used to estimate rates of speciation and extinction. However, simulations of these processes show potential ambiguities in the interpretation of data. Clade size is unrelated to age in many studies, suggesting that speciation and extinction might be in long‐term equilibrium and raising questions about unobserved past diversity. Among passerine birds and other groups, the size of similar‐aged clades is positively related to the size of the region within which they have diversified, and it is greater in tropical than in temperate regions. There is no consensus on the causes of these patterns. Finally, the ecological interactions between populations within regions brings the timescale of species sorting and species production close to each other and emphasizes the important interaction of ecological and evolutionary processes in shaping ecological systems.
Farm Mechanization and Rural Migration in the Great Depression

We study sectoral labor realloaction in the U.S. during the Great Depression by examining transitions between the farm and nonfarm sectors as well as movement within the farm sector. Towns and cities that are hit harder by the downturn see higher levels of out-migration to farms, suggesting that the widespread movement to farms serves as a source of migratory insurance. We also show that the more mechanized farming areas are far less able to provide this insurance function. In fact, while the subsistence agricultural sector gains large numbers of people during the crisis, the mechanized agricultural sector sheds workers. Instead of being released into more productive occupations, many of the workers leaving these mechanized areas are themselves moving into low-productivity or subsistence farming. This evidence suggests that economic downturns can interrupt the process of structural transformation and that the job losses associated with structural change may exacerbate the employment problem during economic downturns.
The 70% Majority: Enduring Consumer Beliefs about Advertising

Six decades of survey data consistently indicate that about 70% of consumers think that advertising is often untruthful, it seeks to persuade people to buy things they do not want, it should be more strictly regulated, and it nonetheless provides valuable information. Consumers also tend to find that advertising's benefits outweigh its deficits. These beliefs have remained remarkably stable despite large fluctuations in the scope and vigor of advertising regulation.
Identifying dynamical instabilities in supply networks using generalized modeling

Abstract Supply networks need to exhibit stability in order to remain functional. Here, we apply a generalized modeling (GM) approach, which has a strong pedigree in the analysis of dynamical systems, to study the stability of real-world supply networks. It goes beyond purely structural network analysis approaches by incorporating material flows, which are defining characteristics of supply networks. The analysis focuses on the network of interactions between material flows, providing new conceptualizations to capture key aspects of production and inventory policies. We provide stability analyses of two contrasting real-world networks? that of an industrial engine manufacturer and an industry-level network in the luxury goods sector. We highlight the criticality of links with suppliers that involve the dispatch, processing, and return of parts or sub-assemblies, cyclic motifs that involve separate paths from a common supplier to a common firm downstream, and competing demands of different end products at specific nodes. Based on a critical discussion of our findings in the context of the supply chain management literature, we generate five propositions to advance knowledge and understanding of supply network stability. We discuss the implications of the propositions for the effective management, control, and development of supply networks. The GM approach enables fast screening to identify hidden vulnerabilities in extensive supply networks.
Evaluation of Lactobacillus rhamnosus strain GG for the prevention of atopic dermatitis in dogs.

OBJECTIVE To evaluate the efficacy of the probiotic Lactobacillus rhamnosus strain GG for the alleviation or prevention of clinical signs of atopic dermatitis (AD) in genetically predisposed dogs. ANIMALS 2 adult Beagles with severe AD and 16 puppies. PROCEDURES The 2 adult Beagles were bred twice, with a year between breedings. Lactobacillus rhamnosus GG was administered to the bitch during the second pregnancy and to the puppies of the second litter from 3 weeks to 6 months of age. Both litters were epicutaneously sensitized to Dermatophagoides farinae. Blood samples were collected from puppies every 6 weeks to measure serum titers of allergen-specific IgE. At 6 months of age, all puppies underwent intradermal allergen testing and environmental challenge with D farinae. Clinical signs were scored. RESULTS In the first litter, at 6 months of age, 7 of 7 puppies were strongly seropositive for IgE against D farinae, 6 had a positive reaction to intradermal testing, and 7 developed severe clinical signs of AD after the environmental challenge. In the second litter, 7 of 9 puppies were seropositive, 3 had a positive reaction to intradermal testing, and 6 developed dermatitis and pruritus after the challenge. The second litter had a significantly lower serum titer of allergen-specific IgE and milder reaction to intradermal testing, compared with the first litter. Clinical scores did not differ between litters. CONCLUSIONS AND CLINICAL RELEVANCE Administration of L rhamnosus GG to puppies appeared to reduce immunologic indicators of AD, although no significant decrease in clinical signs was detected.
Prostaglandin E2 Regulates Cell Migration via the Intracellular Activation of the Epidermal Growth Factor Receptor*

Over the past decade cyclooxygenase-2-derived prostaglandins have been implicated in the development and progression of many types of cancer. Recently our laboratory has shown that treatment with prostaglandin E2 (PGE2) induces increased proliferation, migration, and invasiveness of colorectal carcinoma cells (Sheng, H., Shao, J., Washington, M. K., and DuBois, R. N. (2001) J. Biol. Chem. 276, 18075–18081). The stimulatory effects of PGE2 were dependent upon the activation of the phosphatidylinositol 3-kinase/Akt pathway. However, the exact signaling cascade responsible for phosphatidylinositol 3-kinase/Akt activation by PGE2 remains poorly defined. In the present study, we demonstrate that the PGE2-induced migration and invasion occurs via rapid transactivation and phosphorylation of the epidermal growth factor receptor (EGFR). Within minutes following treatment, PGE2 induces the activation of Akt. This effect was completely abolished by EGFR-specific tyrosine kinase inhibitors providing evidence for the role of the EGFR in this response. The rapid transactivation of the EGFR occurs via an intracellular Src-mediated event but not through the release of an extracellular epidermal growth factor-like ligand. EGFR transactivation was also observed in vivo by the direct comparison of normal and malignant human colorectal samples. These results suggest that in developing colonic carcinomas, the early effects of cyclooxygenase-2-derived PGE2 are in part mediated by the EGFR, and this transactivation is responsible for subsequent down-stream effects including the stimulation of cell migration and invasion.
Mixed-Handedness in Patients with Functional Psychosis

Background An excess of non-right-handedness has been shown among patients with schizophrenia. However it is not clear whether this finding can be accounted for by an increase in left-handedness, mixed-handedness or both. It is not known whether atypical patterns of hand preferences occur in other functional psychotic illnesses. Method The Annett hand preference questionnaire was administered to patients with schizophrenia (n=120); affective psychosis (n=55); schizoaffective psychosis (n=41), and control subjects (n=86). Handedness was classified into three categories: right, mixed and left-handedness. Results The hand preference patterns of patients with functional psychotic illnesses were not significantly different from controls. Patients with schizophrenia showed a non-significant excess of mixed-handedness compared with controls. Patients with schizophrenia and affective psychosis showed a non-significant decrease in left-handedness compared with controls. Conclusions Although our results showed a trend in the hypothesised direction, we failed to demonstrate that patients with psychotic illness differed from controls on self-reported hand preference patterns.
Ambulatory monitoring of physical activity and symptoms in fibromyalgia and chronic fatigue syndrome.

OBJECTIVE Fibromyalgia (FM) and chronic fatigue syndrome (CFS) are associated with substantial physical disability. Determinants of self-reported physical disability are poorly understood. This investigation uses objective ambulatory activity monitoring to compare patients with FM and/or CFS with controls, and examines associations of ambulatory activity levels with both physical function and symptoms during activities of daily life. METHODS Patients with FM and/or CFS (n = 38, mean +/- SD age 41.5 +/- 8.2 years, 74% women) completed a 5-day program of ambulatory monitoring of physical activity and symptoms (pain, fatigue, and distress) and results were compared with those in age-matched controls (n = 27, mean +/- SD age 38.0 +/- 8.6 years, 44% women). Activity levels were assessed continuously, ambulatory symptoms were determined using electronically time-stamped recordings at 5 time points during each day, and physical function was measured with the 36-item Short Form health survey at the end of the 5-day monitoring period. RESULTS Patients had significantly lower peak activity levels than controls (mean +/- SEM 8,654 +/- 527 versus 12,913 +/- 1,462 units; P = 0.003) and spent less time in high-level activities when compared with controls (P = 0.001). In contrast, patients had similar average activity levels as those of controls (mean +/- SEM 1,525 +/- 63 versus 1,602 +/- 89; P = 0.47). Among patients, low activity levels were associated with worse self-reported physical function over the preceding month. Activity levels were inversely related to concurrent ambulatory pain (P = 0.031) and fatigue (P < 0.001). Pain and fatigue were associated with reduced subsequent ambulatory activity levels, whereas activity levels were not predictive of subsequent symptoms. CONCLUSION Patients with FM and/or CFS engaged in less high-intensity physical activities than that recorded for sedentary control subjects. This reduced peak activity was correlated with measures of poor physical function. The observed associations may be relevant to the design of behavioral activation programs, because activity levels appear to be contingent on, rather than predictive of, symptoms.
Genetic characterization of Bactrocera fruit flies (Diptera: Tephritidae) from Northeastern India based on DNA barcodes

Abstract The Northeastern region of India, one of the mega biodiversity hot spots has enormous potential for the production of fruits and vegetables. Fruit flies of the genus Bactrocera Macquart are important pests of fruits and vegetables, and one of the limiting factors in successful production of these commodities. The relationship among some of the species is unclear due to their high molecular and morphological similarities. Moreover, due to the significant morphological resemblance between fruit fly species, reliable identification is very difficult task. We genetically characterized 10 fruit fly species of the genus Bactrocera by using standard DNA barcoding region of COI gene. The characterization and identification of eight species were straight forward. This study was unable to establish the molecular identity of Bactrocera sp. 2. Within the 547 bp region of partial COI gene, there were 157 variable sites of which 110 sites were parsimony informative, 153 were synonymous substitutions and 4 were non-synonymous substitutions. The estimate of genetic divergence among the ten species was in the range of 0–21.9% and the pairwise genetic distance of Bactrocera. (Bactrocera) dorsalis (Hendel) with B. (B.) carambolae was only 0.7%. Phylogenetic analysis formed separate clades for fruit and vegetable infesting fruit flies. B. (B.) aethriobasis Hardy, B. (B.) thailandica and B. (B.) tuberculata (Bezzi) have been reported for the first time from the Northeastern India. The information generated from this study would certainly have implications for pest management, taxonomy, quarantine and trade.
Anatomic and Disease Specificity of NADH CoQ1 Reductase (Complex I) Deficiency in Parkinson's Disease

Abstract: 1‐Methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) is thought to produce parkinsonism in humans and other primates through its inhibition of complex I. The recent discovery of mitochondrial complex I deficiency in the substantia nigra of patients with Parkinson's disease has provided a remarkable link between the idiopathic disease and the action of the neurotoxin MPTP. This article shows that complex I deficiency in Parkinson's disease is anatomically specific for the substantia nigra, and is not present in another neurodegenerative disorder involving the substantia nigra. Evidence is also provided to show that there is no correlation between l‐3,4‐dihydroxyphenylalanine therapy and complex I deficiency. These results suggest that complex I deficiency may be the underlying cause of dopaminergic cell death in Parkinson's disease.
Biochemical and molecular studies using human autopsy brain tissue

The use of human brain tissue obtained at autopsy for neurochemical, pharmacological and physiological analyses is reviewed. RNA and protein samples have been found suitable for expression profiling by techniques that include RT‐PCR, cDNA microarrays, western blotting, immunohistochemistry and proteomics. The rapid development of molecular biological techniques has increased the impetus for this work to be applied to studies of brain disease. It has been shown that most nucleic acids and proteins are reasonably stable post‐mortem. However, their abundance and integrity can exhibit marked intra‐ and intercase variability, making comparisons between case‐groups difficult. Variability can reveal important functional and biochemical information. The correct interpretation of neurochemical data must take into account such factors as age, gender, ethnicity, medicative history, immediate ante‐mortem status, agonal state and post‐mortem and post‐autopsy intervals. Here we consider issues associated with the sampling of DNA, RNA and proteins using human autopsy brain tissue in relation to various ante‐ and post‐mortem factors. We conclude that valid and practical measures of a variety of parameters may be made in human brain tissue, provided that specific factors are controlled.
Sex difference in risk of recurrent venous thrombosis and the risk profile for a second event

Summary Background: The risk of recurrent venous thrombosis is higher in men than in women, and this is so far unexplained. We set out to determine the influence of age, time between first and second event, type of first event, oral contraception, pregnancy and surgery. Methods: We performed a prospective follow‐up study of 474 patients with a first objective diagnosis of deep vein thrombosis, aged 18–70 years (Leiden Thrombophilia Study cohort). Results: During 3477 person‐years of follow‐up, 90 recurrences occurred. The overall incidence rates of recurrence (IRs) were 40.9 per 1000 person‐years in men and 15.8 per 1000 person‐years in women. Men with an unprovoked first event had the highest risk of recurrence, with almost one‐third experiencing a second unprovoked event within 8 years (IR 41.2 per 1000 person‐years). This risk was three‐fold lower in women [IR 14.2 per 1000 person‐years; hazard ratio 2.8 (95% confidence interval 1.4–5.7)]. Age at diagnosis had little effect on recurrence rate, and nor had time elapsed since the first event. In women, almost half of the recurrences were provoked and were mainly related to oral contraceptive use or pregnancy. Conclusions: The higher recurrence rate in men than in women is not the result of differences in the environmental or transient risk factors that we studied. The risk profile for a second thrombotic event is clearly different from that of a first.
The Dimensionality of Coping Among Chinese Health Care Workers

Abstract The transactional model defines coping as a process that changes on the basis of the context of an environmental encounter. An instrument used to investigate coping in diverse person-environment interactions is the Ways of Coping Questionnaire (WOC; S. Folkman & R. S. Lazarus, 1988). Although evidence exists to support the basic underlying structure of the WOC in Western societies, no research has been conducted on the instrument's dimensionality in non-Western societies. The authors identified 14 factors for the WOC administered to a sample of health care workers in Beijing, China. The 14 factors identified in the present study were similar to the 8 factors identified in the original validation study (S. Folkman, R. S. Lazarus, C. Dunkel-Schetter, A. DeLongis, & R. J. Gruen, 1986), but they were more content specific.
PUBS: Pulsatility‐based segmentation of lumens conducting non‐steady flow

Dynamic velocity‐encoded phase‐contrast MRI (PC‐MRI) techniques are being used increasingly to quantify pulsatile flows for a variety of clinical applications. Studies suggest that the reliability of flow quantitation with PC‐MRI appears to be dominated by the consistency in the delineation of the lumen boundary. An automated method that utilizes both spatial and temporal information has been developed for improved accuracy and reproducibility. The method's accuracy was evaluated using a flow phantom with 8‐ and 5‐mm‐diameter lumens at two different flow rates. The reproducibility of the method was further evaluated with arterial, venous, and cerebrospinal fluid flow data from human subjects. The results were compared with measurements obtained manually by observers of different skill levels. Measurement values obtained manually were consistently smaller than those obtained with the pulsatility‐based segmentation (PUBS) method. For the 8‐mm lumen, significant improvements in measurement accuracy were obtained. Average lumen area measurement errors of about 18% for the high and low flows, obtained manually by a skilled observer, were reduced to 2.9% and 4.8%, respectively. For the 5‐mm lumen, the skilled observer underestimated the lumen area by 13%, while the PUBS method overestimated the lumen area by 28%. Overestimated lumen area measurements for the smaller lumen are attributed to the partial‐volume effect. There was significantly less measurement variability with the PUBS method. An average fourfold reduction in interobserver measurement variability was obtained with the new method. Magn Reson Med 49:934–944, 2003. © 2003 Wiley‐Liss, Inc.
Triaging dysphagia: nurse screening for dysphagia in an acute hospital.

AIMS AND OBJECTIVES To (1) develop a dysphagia screening tool to triage all patients at risk of aspiration/dysphagia on admission to acute hospital wards, (2) evaluate tool reliability, (3) evaluate nursing compliance and (4) develop a robust dysphagia training programme. BACKGROUND Failure to diagnose dysphagia has significant medical and economic costs. Dysphagia screening reduces pneumonia threefold. Most nurse-screening tools have focused on stroke. However, many other conditions are associated with dysphagia. A multidisciplinary team developed a nurse-administered, evidence-based swallow screening tool for generic acute hospital use. DESIGN Prospective, quasi-experimental. METHODS Nurses were assessed for knowledge pre- and post-training. All patients were nurse-screened for dysphagia on admission. All patients were reviewed by speech pathologists to determine screening accuracy. Results were not blinded. The one page tool encompassed (1) diagnostic categories, (2) patient/carer interview, (3) dysphagia indicators and (4) if applicable, water swallow test. RESULTS Thirty-eight nurses participated in a seven-week study; 442 patients were screened on two general medical wards. Three speech pathologists counter-assessed each patient by clinical examination or chart review. Sensitivity was 95%; specificity was 97%. Positive predictive value was 92%; negative predictive value was 98%. 3.4% of clinical screening decisions were incorrect. Compliance rate was 85%. CONCLUSIONS Caution is advised in interpretation of the results due to lack of blinding. Initial results suggest that the dysphagia screening tool is a quick and robust tool for triaging individuals with dysphagia. Training is critical to successful screening. RELEVANCE TO CLINICAL PRACTICE Twenty-five to 30% of acute hospitalised individuals have dysphagia. All adult acute patients are screened for dysphagia using the Royal Brisbane and Women's Hospital dysphagia screening tool. Patients are triaged into categories of 'those requiring additional specialist intervention' and 'those who can proceed directly to regular diets and liquids'. Improved quality of care and cost savings is likely.
High efficient anti-cancer drug delivery systems using tea polyphenols reduced and functionalized graphene oxide

Targeted drug delivery is urgently needed for cancer therapy, and green synthesis is important for the biomedical use of drug delivery systems in the human body. In this work, we report two targeted delivery systems for anticancer drugs based on tea polyphenol functionalized and reduced graphene oxide (TPGs). The obtained TPGs demonstrated an efficient doxorubicin loading capacity as high as 3.430 × 106 mg g−1 and 3.932 × 104 mg g−1, and exhibited pH-triggered release. Furthermore, the kinetic models, adsorption isotherms, and possible loading mechanisms were investigated in details. Compared to TPG1 and free doxorubicin, TPG2 is biocompatible to normal cells even at high concentrations and promotes tumor cells death by delivering the doxorubicin mainly to the nuclei. These results were confirmed using cell viability tests and confocal laser microscopy. Moreover, apoptosis tests showed that the mechanism of cancer cell death induced by TPG1 and TPG2 might follow the similar mechanisms. Taken together, these results demonstrate that TPGs provide a multifunctional drug delivery system with a greater loading capacity and pH-sensitive drug release for enhanced cancer therapy. The high drug payload capability and enhanced antitumor efficacy demonstrate that we developed systems are promising for various biomedical applications and cancer therapy.