Miracle-dz/UniFAR-FTUs huggingface dataset:
Quantitative analysis of wound healing

In this article, we initially review several problems associated with the design and interpretation of certain types of experiments currently used to study wound healing, drawing attention to the fact that applications for standard statistical techniques in the analysis of the experimental results are often of limited value. We then argue that, because of the special nature of wound healing data, curve fitting of empirical model equations can often provide a convenient way to summarize treatment effects with large data sets. The various ways in which this technique could be used to facilitate the interpretation of experimental wound healing results are then explored. To illustrate this approach, we then took several wound healing experiments and introduced possible models that could be used, paying particular attention to simple equations with the smallest possible number of parameters. For each equation, the way that the parameters of the model could be interpreted with regard to the biologic effects represented is given. Examples are given to show the application of each model discussed theoretically in the interpretation of some typical experimental data sets.
A vision-based serial number recognition algorithm for HSR trains by nearest neighbor chains of connected components

With the rapid development of High Speed Railway (HSR) in recent years, its research become one hot academic topic. Serial numbers of HSR trains are unique identifications, which play an important role in railway management and operation. In this paper, we present a vision-based algorithm to automatically recognize the serial numbers of HSR trains by image sensors. Firstly, according to the fixed character layout of serial numbers, the serial number regions in the image are located by a combination of connected components. Maximally Stable Extremal Region (MSER) detector is introduced to extract reliable connected components as candidate characters. Based on the pairwise geometry relation between candidates, these connected components constitute the nearest neighbor chain. The accurate location of the serial number is obtained with the nearest neighbor chain. Meanwhile, character images can be segmented simultaneously. Finally, the characters are recognized with Histogram of Gradient (HoG) features and simple similarity-based classifiers. In the experiments, our recognition performance is evaluated by the test images which are collected from real application scenes. The experimental results show the reliability and effectiveness of our serial number recognition algorithm.
MEASURING CREDIT STRUCTURE IMPACT ON ECONOMIC GROWTH IN CROATIA USING (VECM) 1990-2018

Studies on the finance-growth link use different proxy variables for financial development. Among the most used is the total credit share in the GDP. Previous empirical studies show to be sensitive to the choice of the finance proxy indicator. Total credit share in the GDP appears biased in empirical modeling. Credit structure (loans to firms and households) prove to be more robust when used in the modeling. Credit structure reveals a different impact on economic growth showing lending policy impact varies depending on the credit structure. Researchers studying the finance-growth link must account for this when investigating supply leading and demand-following theories. Policymakers should also take care of the credit structure since loans to household discourage growth in the long run and are sensitive to economic shocks. We find empirical evidence to support both supply leading and demand- following theory. Bi-directional causality between private loans to firms/households and economic growth exists using Granger causality test. Private loans to firms and households economic growth exists using Granger causality test. Private loans to firms and households have a positive impact on economic growth in Croatia.
Social Support Promoting Health in Older Women

ABSTRACT Given the aging of women and their increased risk for health problems, it is important to explicate factors that may foster health promotion. While social support has been a concept of interest in predicting health promotion in women, the concept has been inconsistently defined and poorly explicated. A qualitative study was conducted to describe the types and processes of social support for health promotion in older women. Focus group interviews with 51 women, aged 55–93, were held. Data were analyzed using open coding to categorize data with attention to consistency and variance. Data explicated aspects of social support unique to health promotion in community-dwelling older women. Broad categories identified included connectedness and collectivism. Findings validate existing conceptualizations and provide an expanded perspective of social support by demonstrating aspects of support unique to the social context of older, community-dwelling women. Findings also provide a basis for development of socially relevant interventions designed to promote health and optimize health outcomes in older women.
Trial Test of a Bulk-Type Fully HTS Synchronous Motor

The world's first bulk-type fully high temperature superconducting synchronous motor (HTS-SM) was assembled and tested in our laboratory at the University of Cambridge. The fully HTS-SM was designed with 75 Y123 HTS bulks mounted on the surface of the rotor and six air core 2G HTS racetrack coils used for stator windings. We successfully applied a light fan load test for this fully HTS-SM at its operating temperature of 77 K. The detected decay of the trapped magnetic flux densities at the centre of the HTS bulks was up to 16.5% after 5 h of synchronous rotation. Due to the high current density of the HTS material, the ac stator field for the 2G HTS winding was 49.2% stronger compared with a comparable copper winding. In the meantime, we estimated that the efficiency was about 86% potentially under stable low frequency rotation at 150 r/min. The results show that the performance of this HTS motor is acceptable for practical applications.
The 10,400- and 14,500-dalton proteins encoded by region E3 of adenovirus form a complex and function together to down-regulate the epidermal growth factor receptor

In adenovirus-infected cells, the epidermal growth factor receptor (EGF-R) is internalized from the cell surface via endosomes and is degraded, and the E3 10,400-dalton protein (10.4K protein) is required for this effect (C. R. Carlin, A. E. Tollefson, H. A. Brady, B. L. Hoffman, and W. S. M. Wold, Cell 57:135-144, 1989). We now report that both the E3 10.4K and E3 14.5K proteins are required for this down-regulation of EGF-R in adenovirus-infected cells. Down-regulation of cell surface EGF-R was demonstrated by results from several methods, namely the absence of EGF-R autophosphorylation in an immune complex kinase assay, the inability to iodinate EGF-R on the cell surface, the formation of endosomes containing EGF-R as detected by immunofluorescence, and the degradation of the metabolically [35S]Met-labeled fully processed 170K species of EGF-R. No effect on the initial synthesis of EGF-R was observed. This down-regulation was ascribed to the 10.4K and 14.5K proteins through the analysis of cells infected with rec700 (wild-type), dl748 (10.4K-, 14.5K+), or dl764 (10.4K+, 14.5K-) or coinfected with dl748 plus dl764. Further evidence that the 10.4K and 14.5K proteins function in concert was obtained by demonstrating that the 10.4K protein was coimmunoprecipitated with the 14.5K protein by using three different antisera to the 14.5K protein, strongly implying that the 10.4K and 14.5K proteins exist as a complex. Together, these results indicate that the 10.4K and 14.5K proteins function as a complex to stimulate endosome-mediated internalization and degradation of EGF-R in adenovirus-infected cells.
Control of Paip1-Eukayrotic Translation Initiation Factor 3 Interaction by Amino Acids through S6 Kinase

ABSTRACT The simultaneous interaction of poly(A)-binding protein (PABP) with eukaryotic translation initiation factor 4G (eIF4G) and the mRNA 3′ poly(A) tail promotes translation initiation. We previously showed that the interaction of PABP-interacting protein 1 (Paip1) with PABP and eukaryotic translation initiation factor 3 (eIF3; via the eIF3g subunit) further stimulates translation. Here, we demonstrate that the interaction of eIF3 with Paip1 is regulated by amino acids through the mTORC1 signaling pathway. The Paip1-eIF3 interaction is impaired by the mTORC1 inhibitors, rapamycin and PP242. We show that ribosomal protein S6 kinases 1 and 2 (S6K1/2) promote the interaction of eIF3 with Paip1. The enhancement of Paip1-eIF3 interaction by amino acids is abrogated by an S6K inhibitor or shRNA against S6K1/2. S6K1 interacts with eIF3f and, in vitro, phosphorylates eIF3. Finally, we show that S6K inhibition leads to a reduction in translation by Paip1. We propose that S6K1/2 phosphorylate eIF3 to stimulate Paip1-eIF3 interaction and consequent translation initiation. Taken together, these data demonstrate that eIF3 is a new translation target of the mTOR/S6K pathway.
Variables associated with current smoking status in chronic pain patients.

OBJECTIVES Smokers may report more pain and may be at greater risk for psychiatric comorbidity. Smoking may be a major problem in chronic pain patients (CPPs). The goal of this study was to determine if pain and psychiatric comorbidity are associated with smoking status in CPPs. DESIGN As part of a return-to-work grant study CPPs who could potentially return to work identified themselves as either current smokers (N=81) or nonsmokers (N=140). These two groups were compared on a large number of demographic, function, pain, disability, behavior, and psychiatric diagnoses variables gathered at admission into the grant study. The incidence of smoking was tested with either the student's t-test or chi-square to detect differences in continuous and categorical variables, respectively. Logistic regression was utilized to determine the predictive variables for smoking status by inputting significant independent variables (P<0.01) from the prior analyses. SETTING Pain facility. RESULTS Five variables were found to explain 38.8% of the variance for smoking status. These were education; race (Caucasian); cups of coffee per day; a diagnosis of current alcohol abuse/dependence; and personality disorder. CONCLUSIONS Smoking status in CPPs is associated with some variables that are similar for smoking in the general and psychiatric populations (education, race, alcoholism). However, a number of variables expected to be relevant (e.g., mood disorders) were not associated with smoking status in CPPs. These results may not be generalizable to all CPPs as they are derived from CPPs who are return-to-work candidates.
Market Efficiency and Volatility Persistence of Cryptocurrency during Pre- and Post-Crash Periods of Bitcoin: Evidence based on Fractional Integration

This paper investigates both market efficiency and volatility persistence in 12 cryptocurrencies during pre-crash and post-crash periods. We were motivated by the erroneous belief of some authors that driving currency, Bitcoin is inefficient. By considering robust fractional integration methods in linear and nonlinear set up, we found that markets of Bitcoin and most altcoins considered in our samples can be dubbed as efficient, and these are highly volatile particularly in the post-crash sample that we are now. These volatilities will then persist for shorter period than in the pre-crash period. Our work therefore renders important information to cryptocurrency market participants and portfolio managers.
Regional filter heparinization for continuous veno-venous hemofiltration in liver transplant recipients.

AIM To study safety, clinical and operative feasibility of continuous veno-venous hemofiltration (CVVH) with anticoagulation only of the filter in patients at risk for bleeding. METHODS This prospective, comparative, non randomised study was completed at an intensive care unit of a teaching NHS hospital. Sixteen liver transplant (LT) recipients with acute renal failure needing CVVH were treated with a regional anticoagulation protocol (heparin and protamine were administered respectively pre- and post-filter) and compared to 11 critically ill subjects who received a standard low-heparin treatment. Activated coagulation time (ACT) monitoring was used to adjust anticoagulation and heparin neutralization. RESULTS Mean circuit life was 35.8+/-13.6 hours (95% CI 28.5-43.1) in patients receiving regional anticoagulation and 34.4+/-14 hours in controls (95% CI 25.5-43.3; p=0.7). Fourty-eight circuits (47.5% of the total) in the heparin-protamine group had a life-span longer than 30 hours and other 22 (21.7%) were changed intentionally after 24 hours of use in absence of clots. None of the patients in both the studied groups had bleeding or hemodynamic complications and their azotemic control was always satisfactory. CONCLUSION In LT recipients, regional anticoagulation can achieve a circuits life-span comparable to that from systemic anticoagulation with satisfactory safety and simplicity of use.
Hilar interneuron vulnerability distinguishes aged rats with memory impairment

Hippocampal interneuron populations are reportedly vulnerable to normal aging. The relationship between interneuron network integrity and age‐related memory impairment, however, has not been tested directly. That question was addressed in the present study using a well‐characterized model in which outbred, aged, male Long‐Evans rats exhibit a spectrum of individual differences in hippocampal‐dependent memory. Selected interneuron populations in the hippocampus were visualized for stereological quantification with a panel of immunocytochemical markers, including glutamic acid decarboxylase‐67 (GAD67), somatostatin, and neuropeptide Y. The overall pattern of results was that, although the numbers of GAD67‐ and somatostatin‐positive interneurons declined with age across multiple fields of the hippocampus, alterations specifically related to the cognitive outcome of aging were observed exclusively in the hilus of the dentate gyrus. Because the total number of NeuN‐immunoreactive hilar neurons was unaffected, the decline observed with other markers likely reflects a loss of target protein rather than neuron death. In support of that interpretation, treatment with the atypical antiepileptic levetiracetam at a low dose shown previously to improve behavioral performance fully restored hilar SOM expression in aged, memory‐impaired rats. Age‐related decreases in GAD67‐ and somatostatin‐immunoreactive neuron number beyond the hilus were regionally selective and spared the CA1 field of the hippocampus entirely. Together these findings confirm the vulnerability of hippocampal interneurons to normal aging and highlight that the integrity of a specific subpopulation in the hilus is coupled with age‐related memory impairment. J. Comp. Neurol. 521:3508‐3523, 2013. © 2013 Wiley Periodicals, Inc.
Diverse and Novel Oral Bacterial Species in Blood following Dental Procedures

ABSTRACT We identified oral bacterial species in blood cultures following single-tooth extraction and tooth brushing. Sequence analysis of 16S rRNA genes identified 98 different bacterial species recovered from 151 bacteremic subjects. Of interest, 48 of the isolates represented 19 novel species of Prevotella, Fusobacterium, Streptococcus, Actinomyces, Capnocytophaga, Selenomonas, and Veillonella.
Loss of Genomic Imprinting in Mouse Embryos with Fast Rates of Preimplantation Development in Culture1

ABSTRACT Currently, the stage of embryo development has been proposed as one of many criteria for identifying healthy embryos in infertility clinics with the fastest embryos being highlighted as the healthiest. However the validity of this as an accurate criterion with respect to genomic imprinting is unknown. Given that embryo development in culture generally requires an extra day compared to in vivo development, we hypothesized that loss of imprinting correlates with slower rates of embryonic development. To evaluate this, embryos were recovered at the 2-cell stage, separated into four groups based on morphological stage at two predetermined time points, and cultured to blastocysts. We examined cell number, embryo volume, embryo sex, imprinted Snrpn and H19 methylation, imprinted Snrpn, H19, and Cdkn1c expression, and expression of genes involved in embryo metabolism—Atp1a1, Slc2a1, and Mapk14—all within the same individual embryo. Contrary to our hypothesis, we observed that faster developing embryos exhibited greater cell numbers and embryo volumes as well as greater perturbations in genomic imprinting and metabolic marker expression. Embryos with slower rates of preimplantation development were most similar to in vivo derived embryos, displaying similar cell numbers, embryo volumes, Snrpn and H19 imprinted methylation, H19 imprinted expression, and Atp1a1 and Slc2a1 expression. We conclude that faster development rates in vitro are correlated with loss of genomic imprinting and aberrant metabolic marker expression. Importantly, we identified a subset of in vitro cultured embryos that, according to the parameters evaluated, are very similar to in vivo derived embryos and thus are likely most suitable for embryo transfer.
SYLVIAN FISSURE ARTERIOVENOUS MALFORMATIONS: AN APPLICATION OF THE SUGITA CLASSIFICATION TO 28 SURGICAL PATIENTS

OBJECTIVE The Sugita classification for sylvian fissure arteriovenous malformations (AVMs) categorizes them into four types based on nidus location in the fissure: pure, lateral, medial, and deep. This classification scheme is simple and appealing, but is rarely applied. We applied the Sugita classification to a consecutive, single-surgeon experience with 28 patients to better characterize the Sugita subtypes. METHODS Twenty-eight patients with sylvian fissure AVMs were identified from a series of 314 AVM patients treated microsurgically over a 9-year period. According to the Sugita classification, six (21%) AVMs were pure, four (14%) were lateral, nine (32%) were medial, and nine (32%) were deep. Fifteen (54%) patients presented with hemorrhage. RESULTS There was no surgical mortality in this experience. Four (14%) patients had transient deterioration and one (3.6%) patient with a pure sylvian AVM was permanently worse. At late follow-up (mean duration, 20 mo), good outcomes (Modified Rankin Scale scores 0–2) were observed in 25 (89%) patients. CONCLUSION The Sugita classification does not modify the surgical approach or predict patient outcomes with sylvian fissure AVMs, but it does clarify anatomic differences between subtypes. These AVMs are a diverse group, with each subtype having unique relationships between arteries, veins, nidus, and adjacent brain that influence microsurgical technique. Despite their eloquent location and technical challenges, results with microsurgical resection are excellent, making this the preferred treatment option for low-grade AVMs.
Does Real Activities Management Influence Earnings Quality and Stock Returns in Emerging Markets? Evidence from Korea

ABSTRACT We explore the association between real earnings management and the persistence of earnings and cash flows, respectively. Further, we investigate the effect of real earnings management on the relation between current stock returns and future earnings. Using 15,826 firm-year observations listed in the Korean stock market from 2000 to 2015, we find that real earnings management is negatively associated with the persistence of earnings. We also find that real earnings management restricts the persistence of cash flows. Lastly, we find that real earnings management prevents the market from assessing firms’ future earnings reflected in the current stock prices.
Radial Sizing of Lipid Nanotubes Using Membrane Displacement Analysis

We report a novel method for the measurement of lipid nanotube radii. Membrane translocation is monitored between two nanotube-connected vesicles, during the expansion of a receiving vesicle, by observing a photobleached region of the nanotube. We elucidate nanotube radii, extracted from SPE vesicles, enabling quantification of membrane composition and lamellarity. Variances of nanotube radii were measured, showing a growth of 40–56 nm, upon increasing cholesterol content from 0 to 20%.
Examination of the rate of peptide biosynthesis in neuroendocrine cell lines using a stable isotopic label and mass spectrometry

The biosynthesis of neuroendocrine peptides is typically examined by following the rate of appearance of a radioactive amino acid into mature forms of peptides. In the present study, we labeled cell lines with l‐leucine containing 10 deuterium residues (d10‐Leu) and used mass spectrometry to measure the biosynthetic rate of γ‐lipotropin in the AtT‐20 cell line and insulin in the INS‐1 cell line. After 3 h of labeling, both peptides show detectable levels of the d‐labeled form in the cells and media. The relative levels of the d‐labeled forms are greater in the media than in the cells, consistent with previous studies that found that newly synthesized peptides are secreted at a higher rate than older peptides under basal conditions. When AtT‐20 cells were stimulated with KCl or forskolin, the ratio of d‐ to H‐labeled γ‐lipotropin in the medium decreased, suggesting that the older peptide was in a compartment that could be released upon the appropriate stimulation. Overexpression of proSAAS in AtT‐20 cells reduced the ratio of d‐ to H‐labeled γ‐lipotropin, consistent with the proposed role of proSAAS as an endogenous inhibitor of prohormone convertase‐1. Labeling with d10‐Leu was also used to test whether altering the pH of the secretory pathway with chloroquine affected the rate of peptide biosynthesis. In AtT‐20 cells, 30 µm chloroquine for 3 or 6 h significantly reduced the rate of formation of γ‐lipotropin in both cells and media. Similarly, INS‐1 cells treated with 10, 30, or 60 µm chloroquine for 6 h showed a significant decrease in the rate of formation of insulin in both cells and media. These results are consistent with the acidic pH optima for peptide processing enzymes. Stable isotopic labeling with d10‐Leu provides a sensitive method to examine the rate of peptide formation in neuroendocrine cell lines.
Climate change and voltinism in Californian insect pest species: sensitivity to location, scenario and climate model choice

Experimental studies of the impact of climatic change are hampered by their inability to consider multiple climate change scenarios and indeed often consider no more than simple climate sensitivity such as a uniform increase in temperature. Modelling efforts offer the ability to consider a much wider range of realistic climate projections and are therefore useful, in particular, for estimating the sensitivity of impact predictions to differences in geographical location, and choice of climate change scenario and climate model projections. In this study, we used well‐established degree‐day models to predict the voltinism of 13 agronomically important pests in California, USA. We ran these models using the projections from three Atmosphere–Ocean Coupled Global Circulation Models (AOCGCMs or GCMs), in conjunction with the SRES scenarios. We ran these for two locations representing northern and southern California. We did this for both the 2050s and 2090s. We used anova to partition the variation in the resulting voltinism among time period, climate change scenario, GCM and geographical location. For these 13 pest species, the choice of climate model explained an average of 42% of the total variation in voltinism, far more than did geographical location (33%), time period (17%) or scenario (1%). The remaining 7% of the variation was explained by various interactions, of which the location by GCM interaction was the strongest (5%). Regardless of these sources of uncertainty, a robust conclusion from our work is that all 13 pest species are likely to experience increases in the number of generations that they complete each year. Such increased voltinism is likely to have significant consequences for crop protection and production.