Miracle-dz/UniFAR-FTUs huggingface dataset:
In vitro treatment of lipopolysaccharide increases invasion of Pasteurella multocida serotype B:2 into bovine aortic endothelial cells

Pasteurella multocida serotype B:2 causes hemorrhagic septicemia in cattle and buffalo. The invasion mechanism of the bacterium when invading the bloodstream is unclear. This study aimed to characterize the effects of immunomodulatory molecules, namely dexamethasone and lipopolysaccharide, on the invasion efficiency of P. multocida serotype B:2 toward bovine aortic endothelial cells (BAECs) and the involvement of actin microfilaments in the invasion mechanism. The results imply that treatment of BAECs with lipopolysaccharide at 100 ng/mL for 24 h significantly increases the intracellular bacteria number per cell (p < 0.01) compared with those in untreated and dexamethasone-treated cells. The lipopolysaccharide-treated cells showed a significant decrease in F-actin expression and an increase in G-actin expression (p < 0.001), indicating actin depolymerization of BAECs. However, no significant differences were detected in the invasion efficiency and actin filament reorganization between the dexamethasone-treated and untreated cells. Transmission electron microscopy showed that P. multocida B:2 resided in a vacuolar compartment of dexamethasone-treated and untreated cells, whereas the bacteria resided in cellular membrane of lipopolysaccharide-treated cells. The results suggest that lipopolysaccharide destabilizes the actin filaments of BAECs, which could facilitate the invasion of P. multocida B:2 into BAECs.
Towards Long Term Cultivation of Drosophila Wing Imaginal Discs In Vitro

The wing imaginal disc of Drosophila melanogaster is a prominent experimental system for research on control of cell growth, proliferation and death, as well as on pattern formation and morphogenesis during organogenesis. The precise genetic methodology applicable in this system has facilitated conceptual advances of fundamental importance for developmental biology. Experimental accessibility and versatility would gain further if long term development of wing imaginal discs could be studied also in vitro. For example, culture systems would allow live imaging with maximal temporal and spatial resolution. However, as clearly demonstrated here, standard culture methods result in a rapid cell proliferation arrest within hours of cultivation of dissected wing imaginal discs. Analysis with established markers for cells in S- and M phase, as well as with RGB cell cycle tracker, a novel reporter transgene, revealed that in vitro cultivation interferes with cell cycle progression throughout interphase and not just exclusively during G1. Moreover, quantification of EGFP expression from an inducible transgene revealed rapid adverse effects of disc culture on basic cellular functions beyond cell cycle progression. Disc transplantation experiments confirmed that these detrimental consequences do not reflect fatal damage of imaginal discs during isolation, arguing clearly for a medium insufficiency. Alternative culture media were evaluated, including hemolymph, which surrounds imaginal discs during growth in situ. But isolated larval hemolymph was found to be even less adequate than current culture media, presumably as a result of conversion processes during hemolymph isolation or disc culture. The significance of prominent growth-regulating pathways during disc culture was analyzed, as well as effects of insulin and disc co-culture with larval tissues as potential sources of endocrine factors. Based on our analyses, we developed a culture protocol that prolongs cell proliferation in cultured discs.
The Impact of Modeling Overall Argumentation with Tree Kernels

Several approaches have been proposed to model either the explicit sequential structure of an argumentative text or its implicit hierarchical structure. So far, the adequacy of these models of overall argumentation remains unclear. This paper asks what type of structure is actually important to tackle downstream tasks in computational argumentation. We analyze patterns in the overall argumentation of texts from three corpora. Then, we adapt the idea of positional tree kernels in order to capture sequential and hierarchical argumentative structure together for the first time. In systematic experiments for three text classification tasks, we find strong evidence for the impact of both types of structure. Our results suggest that either of them is necessary while their combination may be beneficial.
Adult Children’s Responses to Parent “Stubbornness”

Purpose of the Study People cope with relationship tensions in different ways. One such tension that is frequently reported is how adult children respond to conflicts with their aging parents when they see their parents as insisting, resisting, or persisting in their behaviors-acting in ways commonly attributed to stubbornness. Design and Methods Middle-aged adults (N = 383) completed measures regarding their parents' stubbornness, their responses to that stubbornness, depressive symptoms, positive and negative relationship quality, and support they provide their parents. Multiple regression and multilevel models were utilized to examine whether middle-aged offspring's responses to perceived parent stubbornness were associated with the offspring's reported depressive symptoms, positive and negative relationship quality, and provision of support. Results Adult children most frequently endorsed responding to parents' "stubborn" behaviors by "just letting their requests go. " Results revealed associations of adult children's response strategies with their reports of depressive symptoms (more letting go), positive relationship quality (less letting go and more reasoning), negative relationship quality (more arguing, more rewording their request, and less reasoning), and the amount of support they provide to their parents (more arguing, more reasoning, and more waiting to address the concern another day). Implications Adult children's responses to perceived parent stubbornness are linked to individual and relationship functioning, as well as the support the children provide. Interventions to develop adaptive responses when there are differences in goals may prove useful for families.
“That’s What ‘College Experience’ is”: Exploring Cultural Narratives and Descriptive Norms College Students Construct for Legitimizing Alcohol Use

ABSTRACT Among the many health issues relevant to college students, overconsumption of alcohol remains a prevalent concern. Circumscribing the drinking habits occurring on campuses are innumerable social norms furthering popular beliefs that alcohol is an inherent part of college life. This case study examines how students encounter, interpret and express the social norms they conceive as constituting their campus culture. Through triangulating ethnographic practices with in-depth interviews, we utilize a narrative lens to explore how students construct meanings and values for alcohol’s roles in campus life. Our findings demonstrate how students validate uses of alcohol by coupling its presence with stress, social enhancement, peer responsibility and identity. Moreover, interviewees expressed there being subcultural differences between younger and older students’ discourse around alcohol use. We draw from these observations to offer suggestions for engaging students actively in campus interventions that give rise to voices excluded from commonly construed social norms.
Entropy-based metrics for the analysis of partial and total occlusion in video object tracking

Metrics measuring tracking reliability under occlusion that are based on mutual information and do not resort to ground truth data are proposed. Metrics for both the initialisation of the region to be tracked as well as for measuring the performance of the tracking algorithm are presented. The metrics variations may be interpreted as a quantitative estimate of changes in the tracking region due to occlusion, sudden movement or deformation of the tracked object. Performance metrics based on the Kullback -Leibler distance and normalised correlation were also added for comparison purposes. The proposed approach was tested on an object tracking scheme using multiple feature point correspondences. Experimental results have shown that mutual information can effectively characterise object appearance and reappearance in many computer vision applications.
Concurrent exercise on a gravity-independent device during simulated microgravity.

PURPOSE The objective of this study is to examine the effect of a high-intensity concurrent training program using a single gravity-independent device on maintaining skeletal muscle function and aerobic capacity during short-term unilateral lower limb suspension (ULLS). METHODS Nineteen subjects (10 males and 9 females; 21.0 ± 2.5 yr, 65.4 ± 12.2 kg) were separated into two groups: 1) 10-d ULLS only (n = 9) and 2) 10-d ULLS plus aerobic and resistance training (ULLS + EX, n = 10). Exercise was performed on a single gravity-independent Multi-Mode Exercise Device (M-MED) with alternating days of high-intensity interval aerobic training and maximal exertion resistance training. RESULTS Aerobic capacity increased by 7% in ULLS + EX (P < 0.05). Knee extensor and ankle plantar flexor three-repetition maximum increased in the ULLS + EX group (P < 0.05), but this change was only different from ULLS in the plantar flexors (P < 0.05). Peak torque levels decreased with ULLS but were increased for the knee extensors and attenuated for the ankle plantar flexors with ULLS + EX (P < 0.05). A shift toward type IIx myosin heavy-chain mRNA occurred with ULLS and was reversed with ULLS + EX in the vastus lateralis (P < 0.05) but not the soleus. Myostatin and atrogin increased with ULLS in both the vastus lateralis and soleus, but this change was mitigated with ULLS + EX only in the vastus lateralis (P = 0.0551 for myostatin, P < 0.05 for atrogin). Citrate synthase was decreased in the soleus during ULLS but was increased with ULLS + EX (P < 0.05). CONCLUSION These results indicate that an M-MED class countermeasure device appears to be effective at mitigating the deconditioning effects of microgravity simulated during a modified ULLS protocol.
Converging evolution leads to near maximal junction diversity through parallel mechanisms in B and T cell receptors

T and B cell receptor (TCR and BCR) complementarity determining region 3 (CDR3) genetic diversity is produced through multiple diversification and selection stages. Potential holes in the CDR3 repertoire were argued to be linked to immunodeficiencies and diseases. In contrast with BCRs, TCRs have practically no Dβ germline genetic diversity, and the question emerges as to whether they can produce a diverse CDR3 repertoire. In order to address the genetic diversity of the adaptive immune system, appropriate quantitative measures for diversity and large-scale sequencing are required. Such a diversity method should incorporate the complex diversification mechanisms of the adaptive immune response and the BCR and TCR loci structure. We combined large-scale sequencing and diversity measures to show that TCRs have a near maximal CDR3 genetic diversity. Specifically, TCR have a larger junctional and V germline diversity, which starts more 5′ in Vβ than BCRs. Selection decreases the TCR repertoire diversity, but does not affect BCR repertoire. As a result, TCR is as diverse as BCR repertoire, with a biased CDR3 length toward short TCRs and long BCRs. These differences suggest parallel converging evolutionary tracks to reach the required diversity to avoid holes in the CDR3 repertoire.
No Clear Effect of Admixture between Two European Invading Outbreaks of Diabrotica virgifera virgifera in Natura

In this study, we challenged the hypothesis that admixture may have had a positive impact in the context of the European invasion of the western corn rootworm (WCR), Diabrotica virgifera virgifera, LeConte. This beetle was introduced in Europe from the USA several times since the 1980’s. The multiple introductions of this major pest of cultivated corn led to the formation of two major outbreaks in North Western (NW) Italy and in Central and South Eastern (CSE) Europe that eventually merged into a secondary contact zone where insects from both outbreaks interbreed. We collected about 600 insects from this contact zone and genotyped them using 13 microsatellite markers. Three types of information were obtained from the collected individuals: (i) their survival under starvation; (ii) their admixed status, determined through a Bayesian method of genetic clustering and (iii) their mating probability, studied via the detection, isolation and genotyping of sperm in female spermathecae. Twenty six % and 12% of the individuals were assigned to the NW Italy or the CSE Europe parental types, respectively, and 23% and 39% to the F1 and backcross hybrid types, respectively. Globally, our results do not reveal any significant impact of the admixed status on the mating probability and on the choice of mating partners. However the admixed status had a sex- and sampling site-dependent effect on survival in adults under starvation. In addition sex had an effect on survival, with mortality hazard about 3 times larger in males than in females. The consequences of these findings for the evolution of the admixture zone of northern Italy are discussed.
Phasic‐like stimulation of the medial forebrain bundle augments striatal gene expression despite methamphetamine‐induced partial dopamine denervation

Methamphetamine‐induced partial dopamine depletions are associated with impaired basal ganglia function, including decreased preprotachykinin mRNA expression and impaired transcriptional activation of activity‐regulated, cytoskeleton‐associated (Arc) gene in striatum. Recent work implicates deficits in phasic dopamine signaling as a potential mechanism linking methamphetamine‐induced dopamine loss to impaired basal ganglia function. This study thus sought to establish a causal link between phasic dopamine transmission and altered basal ganglia function by determining whether the deficits in striatal neuron gene expression could be restored by increasing phasic dopamine release. Three weeks after pretreatment with saline or a neurotoxic regimen of methamphetamine, rats underwent phasic‐ or tonic‐like stimulation of ascending dopamine neurons. Striatal gene expression was examined using in situ hybridization histochemistry. Phasic‐like, but not tonic‐like, stimulation induced immediate‐early genes Arc and zif268 in both groups, despite the partial striatal dopamine denervation in methamphetamine‐pretreated rats, with the Arc expression occurring in presumed striatonigral efferent neurons. Phasic‐like stimulation also restored preprotachykinin mRNA expression. These results suggest that disruption of phasic dopamine signaling likely underlies methamphetamine‐induced impairments in basal ganglia function, and that restoring phasic dopamine signaling may be a viable approach to manage long‐term consequences of methamphetamine‐induced dopamine loss on basal ganglia functions.
A Qualitative Examination of Physician Gender and Parental Status in Pediatric End-of-Life Communication

ABSTRACT In this study we utilized the framework of patient-centered communication to explore the influence of physician gender and physician parental status on (1) physician-parent communication and (2) care of pediatric patients at the end of life (EOL). The findings presented here emerged from a larger qualitative study that explored physician narratives surrounding pediatric EOL communication. The current study includes 17 pediatric critical care and pediatric emergency medicine physician participants who completed narrative interviews between March and October 2012 to discuss how their backgrounds influenced their approaches to pediatric EOL communication. Between April and June of 2013, participants completed a second round of narrative interviews to discuss topics generated out of the first round of interviews. We used grounded theory to inform the design and analysis of the study. Findings indicated that physician gender is related to pediatric EOL communication and care in two primary ways: (1) the level of physician emotional distress and (2) the way physicians perceive the influence of gender on communication. Additionally, parental status emerged as an important theme as it related to EOL decision-making and communication, emotional distress, and empathy. Although physicians reported experiencing more emotional distress related to interacting with patients at the EOL after they became parents, they also felt that they were better able to show empathy to parents of their patients.
The Study of Organizational Justice, Violation of Psychological Contract and its Effect on Job Satisfaction in Paints Industry of Pakistan

Human resource is a back bone of every organization, without employee no work can be done. So employee’s satisfaction is very important in all terms. Employees will be more satisfied if they get what they expected. When employees are treated fairly overall in the organization, they feel need of reciprocal response to the organization in positive behaviors. Organizational justice and psychological contracts are important factors that influence the satisfactions of the personal in an organization. The purpose of this study is to investigate the relationship among organizational justice (Procedural, Distributional and Interactional), psychological contract and job satisfaction. This paper examines the effect of Job satisfaction on Organizational justice and explains the concept of psychological contract and presents the result of a survey of managers and officers from the paints industry of Pakistan. Data was gathered from 100 employees in the paints industry of Pakistan through the survey method by developing questionnaire. The questionnaire contained the items of all the variables used in the study, for the reason to get the correct results. The data collected was analyzed by using SPSS 17.0. The results revealed a negative relationship between psychological contract violation with both job satisfaction r = -. 130 at p = . 008 and distributional justice Value of r =-0.115 at p=0.254.
Implicit Processing of Visual Emotions Is Affected by Sound-Induced Affective States and Individual Affective Traits

The ability to recognize emotions contained in facial expressions are affected by both affective traits and states and varies widely between individuals. While affective traits are stable in time, affective states can be regulated more rapidly by environmental stimuli, such as music, that indirectly modulate the brain state. Here, we tested whether a relaxing or irritating sound environment affects implicit processing of facial expressions. Moreover, we investigated whether and how individual traits of anxiety and emotional control interact with this process. 32 healthy subjects performed an implicit emotion processing task (presented to subjects as a gender discrimination task) while the sound environment was defined either by a) a therapeutic music sequence (MusiCure), b) a noise sequence or c) silence. Individual changes in mood were sampled before and after the task by a computerized questionnaire. Additionally, emotional control and trait anxiety were assessed in a separate session by paper and pencil questionnaires. Results showed a better mood after the MusiCure condition compared with the other experimental conditions and faster responses to happy faces during MusiCure compared with angry faces during Noise. Moreover, individuals with higher trait anxiety were faster in performing the implicit emotion processing task during MusiCure compared with Silence. These findings suggest that sound-induced affective states are associated with differential responses to angry and happy emotional faces at an implicit stage of processing, and that a relaxing sound environment facilitates the implicit emotional processing in anxious individuals.
Multi-label Topic Classification of Turkish Sentences Using Cascaded Approach for Dialog Management System

In this paper, we propose a two-stage system which aims to classify utterances of customers into 10 categories including daily language and specific banking problems. Detecting the topic of customer question would enable the dialogue system to find the corresponding answer more effectively. In order to identify the topic of customer questions, we built a machine learning based model consisting of two stages which uses feature sets extracted from text and dialogue attributes. In the first stage, where utterances are categorized into two classes, namely daily language and banking domain, the binary classification problemwas studied and different learning algorithms such as Naive Bayes, C4.5 have been evaluated with different feature sets. Utterances classified as banking domain by the first classifier, are classified by second stage classifier. In this stage, automatic detection of specialty of banking-related sentences is aimed. We approached this as a multi-label classification problem. Our proposed cascaded approach has shown a quite good performance with the score of 0.558 in terms of Microaveraged F-score measure.
Knowledge-Guided Robust MRI Brain Extraction for Diverse Large-Scale Neuroimaging Studies on Humans and Non-Human Primates

Accurate and robust brain extraction is a critical step in most neuroimaging analysis pipelines. In particular, for the large-scale multi-site neuroimaging studies involving a significant number of subjects with diverse age and diagnostic groups, accurate and robust extraction of the brain automatically and consistently is highly desirable. In this paper, we introduce population-specific probability maps to guide the brain extraction of diverse subject groups, including both healthy and diseased adult human populations, both developing and aging human populations, as well as non-human primates. Specifically, the proposed method combines an atlas-based approach, for coarse skull-stripping, with a deformable-surface-based approach that is guided by local intensity information and population-specific prior information learned from a set of real brain images for more localized refinement. Comprehensive quantitative evaluations were performed on the diverse large-scale populations of ADNI dataset with over 800 subjects (55∼90 years of age, multi-site, various diagnosis groups), OASIS dataset with over 400 subjects (18∼96 years of age, wide age range, various diagnosis groups), and NIH pediatrics dataset with 150 subjects (5∼18 years of age, multi-site, wide age range as a complementary age group to the adult dataset). The results demonstrate that our method consistently yields the best overall results across almost the entire human life span, with only a single set of parameters. To demonstrate its capability to work on non-human primates, the proposed method is further evaluated using a rhesus macaque dataset with 20 subjects. Quantitative comparisons with popularly used state-of-the-art methods, including BET, Two-pass BET, BET-B, BSE, HWA, ROBEX and AFNI, demonstrate that the proposed method performs favorably with superior performance on all testing datasets, indicating its robustness and effectiveness.