Miracle-dz/UniFAR-FTUs huggingface dataset:
Detection of Viral Antigens in Formalin‐fixed Specimens by Enzyme Treatment

Enzyme treatment (protease or trypsin) was applied to formalin-fixed paraffin-embedded materials and virus-infected cultured cells to detect viral antigens by immunofluorescence. The viral antigens were demonstrated in several organs of autopsy or biopsy cases of which diagnoses had been established by immunofluorescence or virus isolation using frozen materials, or suspected on the basis of serology and/or histopathological findings. These included herpes simplex, varicella-zoster, cytomegalo, subacute sclerosing panencephalitis, progressive multifocal leukoencephalopathy, Japanese B encephalitis, measles, acute hemorrhagic conjunctivitis, Lassa and Korean hemorrhagic fever. Antigen could be recovered also in virus-infected cells (herpes simplex, measles, Lassa, Ebola, Marburg, Rift Valley, Congo and Korean Hemorrhagic fever) by enzyme treatment after periods of formalin fixation of four weeks and storage of three months. In herpes simplex virus-infected mouse brain, antigen was detected after fixation for three months in formalin.
Quality of Life of Iranian Vocational Students with and without Intellectual Disability

The purpose of the present study was to compare the attitudes of individuals with and without intellectual disability toward their quality of life. Associations between demographic variables such as sex, age, father's education, and father's occupation and quality of life were also assessed. 40 people with intellectual disability from special schools were matched with 40 people from Shiraz Vocational Teaching Center on sex, age, and fathers' careers and educations. There was no significant difference between the two groups on total Quality of Life scores, but differences were found in subscales. The groups with intellectual disability reported higher scores on the dimensions of life satisfaction, competency/productivity, and social belongingness/community integration. The normal group scored higher on empowerment/independence. In the intellectual disability group, the father's career was a better predictor of quality of life.
A 0.13 /spl mu/m CMOS front-end, for DCS1800/UMTS/802.11b-g with multiband positive feedback low-noise amplifier

This paper presents a fully integrated CMOS receiver front-end based on a direct conversion architecture for UMTS/802.11b-g and a low-IF architecture at 100 kHz for DCS1800. The two key building blocks are a multiband low-noise amplifier (LNA) that uses positive feedback to improve its gain and a highly linear mixer. The front-end, integrated in a 0.13 /spl mu/m CMOS process, exhibits a minimum noise figure of 5.2 dB, a programmable gain that can be varied from 13.5 to 28.5 dB, an IIP3 of more than -7.5 dBm and an IIP2 better than 50 dBm. The total current consumption is 20mA from a 1.2V supply.
Transcription of the histone H5 gene is regulated by three differentiation-specific enhancers

Histone H5, an early marker of the avian erythroid lineage, is expressed at low levels in early erythroid precursors and at higher levels in more mature cells. We show that the increase in H5 expression is due to transcriptional activation of the H5 gene following differentiation of precursor CFU(E). We have found and characterized two upstream enhancers, E1 (between -2233 and -1878 from the site of transcription initiation, +1) and E3 (between -1321 and -1163), and confirmed the presence of a downstream enhancer (C. D. Trainor, S. J. Stamler, and J. D. Engel, Nature [London] 328:827-830, 1987) E7 (between +846 and +1181) which are responsible for the increase in H5 gene transcription. The enhancers had a weak effect in nondifferentiated CFU(E) but a strong effect when the cells were induced to differentiate. Cooperation among the three enhancers, however, was not required for H5 gene activity in the differentiated cells. The enhancers contain binding sites for several ubiquitous and erythroid cell-specific nuclear proteins, including GATA-1, as demonstrated with GATA-1-specific antibodies. Although the GATA sites were required for enhancer function, the concentration of GATA-1, GATA-2, and GATA-3 decreased during cell differentiation, and overexpression of these factors had little effect on H5 transcription. Hence, the differentiation-specific effect of the enhancers is not mediated by changes in relative levels of the GATA factors. Functional analysis of the H5 promoter indicated that the requirement of several elements, including a GC box necessary for transcription enhancement, did not change during the early stages of CFU(E) differentiation. However, the UPE, a positive element in proliferating CFU(E) recognized by the transcription factor H4TF2, was dispensable in the differentiated cells. These results suggest that as the cells enter the final stages of differentiation, there is a reprogramming of the regulatory factors that control H5 transcription and that the enhancers rescue and increase the activity of the promoter.
When Scenes Fade

Every Wednesday night is Dyke Night in the small cluster of suburbs collectively referred to as Newtown, approximately 5 kms from Sydney's centre. Dyke Night's popularity is evidenced by the groups of women seen weaving their way through the congested sidewalks that link the numerous venues temporarily catering to queer patronage. Drag king performances – a subcultural phenomenon where women consciously perform masculinity before a primarily lesbian audience – became an integral part of the evening between 2002 and 2012, providing a highly visible spectacle that drew women to the series of events that came to designate Sydney's drag king scene. Set within the social life of Dyke Night, this decade-long scene provided the opportunity and justification for mid-week nights out with friends, lovers and fellow fans, as well as offering the potential to bind individuals to the shared pleasures of the performances. But what happens when this scene fades? Looking back on ethnographic research conducted in the moment of its decline, I explore the points of intersection between participants' ephemeral experiences of drag king events and the collective forms of investment that establish it as an intelligible social phenomenon. Data gathered from a series of group discussions reveals the work participants perform of looking backwards from their present position, suggesting that Sydney's drag king scene is only recognizable through anecdotal narration that generates shared meaning of the more mundane forms of sociality that took place. In offering the perspective of a contemporary social moment realized as an historical investment, this article highlights the particular temporal conditions that structure all scenes: their emergence through to their expansion or contraction and, inevitably, their fading.
Cometabolic degradation of chlorinated alkenes by alkene monooxygenase in a propylene-grown Xanthobacter strain

Propylene-grown Xanthobacter cells (strain Py2) degraded several chlorinated alkenes of environmental concern, including trichloroethylene, 1-chloroethylene (vinyl chloride), cis- and trans-1,2-dichloroethylene, 1,3-dichloropropylene, and 2,3-dichloropropylene. 1,1-Dichloroethylene was not degraded efficiently, while tetrachloroethylene was not degraded. The role of alkene monooxygenase in catalyzing chlorinated alkene degradations was established by demonstrating that glucose-grown cells which lack alkene monooxygenase and propylene-grown cells in which alkene monooxygenase was selectively inactivated by propyne were unable to degrade the compounds. C2 and C3 chlorinated alkanes were not oxidized by alkene monooxygenase, but a number of these compounds were inhibitors of propylene and ethylene oxidation, suggesting that they compete for binding to the enzyme. A number of metabolites enhanced the rate of degradation of chlorinated alkenes, including propylene oxide, propionaldehyde, and glucose. Propylene stimulated chlorinated alkene oxidation slightly when present at a low concentration but became inhibitory at higher concentrations. Toxic effects associated with chlorinated alkene oxidations were determined by measuring the propylene oxidation and propylene oxide-dependent O2 uptake rates of cells previously incubated with chlorinated alkenes. Compounds which were substrates for alkene monooxygenase exhibited various levels of toxicity, with 1,1-dichloroethylene and trichloroethylene being the most potent inactivators of propylene oxidation and 1,3- and 2,3-dichloropropylene being the most potent inactivators of propylene oxide-dependent O2 uptake. No toxic effects were seen when cells were incubated with chlorinated alkenes anaerobically, indicating that the product(s) of chlorinated alkene oxidation mediates toxicity.
An improved multilayer self-organizing background subtraction algorithm for microorganism detection in sewage

In this paper, a new image sequence model, obtained by learning in a multilayer self-organizing neural network, is proposed for moving microorganism detection in sewage treatment system. The model is able to handle diverse challenging scenarios accurately, such as dynamic background, gradual illumination variations, shadows cast and so on, which are robust against false detections for different types of micro-videos. Experimental results demonstrate its effectiveness compared with other state-of-the-art methods.
Interactions between Insulin, Body Fat, and Insulin-Like Growth Factor Axis Proteins

Background: The etiology of hormonally related cancers, such as breast and colon, has been linked to hyperinsulinemia and insulin resistance, the insulin-like growth factor (IGF) axis, and obesity. Methods: Data from 57 women (ages 30-50 years) were used to observationally examine cross-sectional and longitudinal relations between body fat (from dual-energy X-ray absorptiometry), insulin, IGF-I, and IGF-binding proteins (IGFBP-1, IGFBP-2, and IGFBP-3). Results: At baseline, participants who had greater than median body fat and insulin levels, >39% and >4.5 microunits/mL, respectively, had 2.3- to 2.6-fold lower IGFBP-1 (P < 0.004) and 1.9- to 2.0-fold lower IGFBP-2 (P < 0.004) compared with other participants; IGF-I and IGFBP-3 levels did not differ by body fat or insulin levels. Over 39 weeks, a 1 microunit/mL reduction in fasting insulin was associated with a 17% increase in IGFBP-1 (P = 0.02) and a 24% increase in IGFBP-2 (P = 0.02) compared with participants who did not reduce insulin; 2.0% loss of body fat over time did not alter IGFBP-1 or IGFBP-2 levels after adjustment for insulin. IGF-I and IGFBP-3 did not change in participants who lost body fat percentage or insulin over time. Conclusions: These observational associations are consistent with the hypothesis that elevated insulin and body fat are associated with decreased IGFBP-1 and IGFBP-2 levels cross-sectionally; they further imply that IGFBP-1 and IGFBP-2 levels may be altered through change in insulin over time. By contrast, no cross-sectional or longitudinal associations were noted between IGF-I and IGFBP-3 with insulin or body fat. (Cancer Epidemiol Biomarkers Prev 2007;16(3):593–7)
Facies classification from well logs using an inception convolutional network

The idea to use automated algorithms to determine geological facies from well logs is not new (see e.g Busch et al. (1987); Rabaute (1998)) but the recent and dramatic increase in research in the field of machine learning makes it a good time to revisit the topic. Following an exercise proposed by Dubois et al. (2007) and Hall (2016) we employ a modern type of deep convolutional network, called \textit{inception network} (Szegedy et al., 2015), to tackle the supervised classification task and we discuss the methodological limits of such problem as well as further research opportunities.
Técnicas multivariantes para el enlace de encuestas

Este trabajo aborda el problema de imputacion en dos encuestas que sobre distintos temas se han realizado en una misma poblacion, cara a enlazar la informacion que contienen, Las encuestas tienen un grupo de variables en comun y cada una serie, ademas, otro grupo de variables especificas. Se estudian tecnicas de insercion y fusion. La insercion se realiza mediante metodos de analisis factorial descriptivo mutlivariante. Consiste en proyectar las variables especificas de las dos encuestas sobre un subespacio comun de representacion, formado por los ejes obtenidos del analisis de las variables comunes.Se estudian formas de determinar si existe ese subespacio comun de representacion. Describimos dos metodos de insercion e ilustramos su funcionamiento insertando los dos ficheros que contienen los datos de la Encuesta de Presupuestos de Tiempo. EPT (realizada en la Comunidad Autonoma Vasca, en los anos 1992-1993). Por ultimo, mediante bootstrap parcial, estudiamos la estabilidad de los resultados de esta insercion. Fusionar encuestas consiste en imputar los valores de las variables especificas de una encuesta para los individuos de la otra, en funcion de los valores de las variables comunes. Es un caso particular de analisis de datos incompletos. Por ello, se estudia la aplicacion de varios metodos de analisis de datos no completos a la fusion de encuestas. Proponemos dos metodos para llevar a cabo la imputacion conjunta mediante arboles de regresion y/o clasificacion. Ambos se aplican a datos de EPT. Se realiza imputacion multiple. Se evaluan los resultados y se comparan con los logrados al repetir el mismo experimento de imputacion utilizando redes neuronales.Por utlimo, se estudia el comportamiento de los metodos de imputacion mediantes arboles (metodos no parametricos) en relacion a un metodo parametrico en diferentes situaciones.Para ello, se aplicabamos tipos de tecnicas apra compeltar
Chronic sleep restriction disrupts interendothelial junctions in the hippocampus and increases blood–brain barrier permeability

Chronic sleep loss in the rat increases blood–brain barrier permeability to Evans blue and FITC‐dextrans in almost the whole brain and sleep recovery during short periods restores normal blood–brain barrier permeability. Sleep loss increases vesicle density in hippocampal endothelial cells and decreases tight junction protein expression. However, at the ultrastructural level the effect of chronic sleep loss on interendothelial junctions is unknown. In this study we characterised the ultrastructure of interendothelial junctions in the hippocampus, the expression of tight junction proteins, and quantified blood–brain barrier permeability to fluorescein‐sodium after chronic sleep restriction. Male Wistar rats were sleep restricted using the modified multiple platform method during 10 days, with a daily schedule of 20‐h sleep deprivation plus 4‐h sleep recovery at their home‐cages. At the 10th day hippocampal samples were obtained immediately at the end of the 20‐h sleep deprivation period, and after 40 and 120 min of sleep recovery. Samples were processed for transmission electron microscopy and western blot. Chronic sleep restriction increased blood–brain barrier permeability to fluorescein‐sodium, and decreased interendothelial junction complexity by increasing the frequency of less mature end‐to‐end and simply overlap junctions, even after sleep recovery, as compared to intact controls. Chronic sleep loss also induced the formation of clefts between narrow zones of adjacent endothelial cell membranes in the hippocampus. The expression of claudin‐5 and actin decreased after chronic sleep loss as compared to intact animals. Therefore, it seems that chronic sleep loss disrupts interendothelial junctions that leads to blood–brain barrier hyperpermeability in the hippocampus.
High coupling coefficient Temperature compensated FBAR resonator for oscillator application with wide pulling range

This paper demonstrates two variations of Temperature compensated (TempCo) FBAR resonators with high Kt<sup>2</sup>. One 1.5 GHz non-symmetric stack design TempCo FBAR resonator has a Kt<sup>2</sup> of 4.28% and linear TCF of 0 ppm/°C. A second, quasi-symmetric stack design 1.5GHz TempCo FBAR resonator has Kt<sup>2</sup> as high as 5.6% and linear TCF of −6 ppm/°C. Significant Kt<sup>2</sup> improvement comes from optimal design of stack film, interposer electrode effect and novel process development of a sealant for the oxide to protect it from HF etching. This paper also discusses the trade-off between two parameters (linear TCF vs. Kt<sup>2</sup>). High Kt<sup>2</sup> TempCo FBAR resonator is ideal for FBAR oscillator application with wide frequency pulling range.
A study on design and analysis of virtual fixtures for cutting in training environments

The escalating use of share-controlled applications, virtual training environments and tele-manipulated devices has motivated the application of force-feedback assistance. In this paper, we present and compare a set of geometrically-based virtual fixtures that provide graphic and force-feedback assistance for path-following task. Two types of force fields (i.e. attractive and reactive) defined within the virtual fixtures were implemented. A preliminary study on these fixtures suggests improvements in performance when force cues are provided through these entities.
Physician characteristics and variability of erythropoiesis-stimulating agent use among Medicare patients with cancer.

6020 Background: Often drugs are approved for one indication based on data from randomized clinical trials, however, once approved, these treatments are used in a different manner than tested. We performed a population-based analysis to determine the patterns of use of erythropoiesis-stimulating agent (ESAs). METHODS We used the SEER-Medicare database to identify subjects >65 years old with breast, lung and colon cancer, diagnosed between1995-2005 who had at least 1 ESA and chemotherapy claim. The associations of patient, tumor, and physician-related factors with receipt of ESA were analyzed. Misuse (< 1 week), prolonged use (> 14 weeks) and off-label use (use while off chemotherapy) were examined. RESULTS Among 21,091 patients analyzed, 5099 (24.2%) received ESA for <1 week (misuse) and 1601 (7.6%) received ESA for >14 weeks (prolonged use). Receipt of ESA while not actively receiving chemotherapy (off-label) occurred in 2876 (13.6%) patients. In a multivariable analysis, ESA misuse was associated with M.D. degree, female physician gender, and earlier year of medical school graduation. Private practice physicians (OR=0.78; 95% CI, 0.72-0.85) and high-volume physicians (OR=0.78; 95% CI, 0.72-0.85) were less likely to use <1 week of ESA. Treatment by a high-volume oncologist (OR=1.35; 95% CI, 1.16-1.58) and by oncologists who graduated from U.S. medical schools (OR=1.35; 95% CI, 1.20-1.51) predicted prolonged duration ESA use, while female oncologists (OR=0.80;95% CI, 0.68-0.94) were less likely to prescribe prolonged ESA. Private practice physicians (OR=1.20; 95% CI 1.03-1.40) and high-volume providers (OR=1.58; 95% CI, 1.34-1.87) were more likely to prescribe > 24 weeks of ESA. CONCLUSIONS We noted widespread variability in the use of erythropoiesis-stimulating agents. Short treatment duration that provides little clinical efficacy, prolonged use, and use while off chemotherapy were common. While patient-related factors influence the patterns of erythropoiesis-stimulating agent use, physician characteristics exerted substantial influence on the way ESAs were administered.
Gastric emptying time of fluids and solids in healthy subjects determined by 13C breath tests: influence of age, sex and body mass index

Background and Aim: Disturbance of gastric emptying leads to a variety of symptoms. Furthermore, gastric motility disorders might play a role in the pathophysiology of functional dyspepsia. In previous studies 13C breath tests were validated as non‐invasive tools in the measurement of gastric emptying time. So far, reliable reference values of healthy subjects are missing and the impact of constitutional traits (age, sex, body mass index [BMI]) needs to be clarified.
Validation of the automated method VIENA: An accurate, precise, and robust measure of ventricular enlargement

Background: In many retrospective studies and large clinical trials, high‐resolution, good‐contrast 3DT1 images are unavailable, hampering detailed analysis of brain atrophy. Ventricular enlargement then provides a sensitive indirect measure of ongoing central brain atrophy. Validated automated methods are required that can reliably measure ventricular enlargement and are robust across magnetic resonance (MR) image types. Aim: To validate the automated method VIENA for measuring the percentage ventricular volume change (PVVC) between two scans. Materials and Methods: Accuracy was assessed using four image types, acquired in 15 elderly patients (five with Alzheimer's disease, five with mild cognitive impairment, and five cognitively normal elderly) and 58 patients with multiple sclerosis (MS), by comparing PVVC values from VIENA to manual outlining. Precision was assessed from data with three imaging time points per MS patient, by measuring the difference between the direct (one‐step) and indirect (two‐step) measurement of ventricular volume change between the first and last time points. The stringent concordance correlation coefficient (CCC) was used to quantify absolute agreement. Results: CCC of VIENA with manual measurement was 0.84, indicating good absolute agreement. The median absolute difference between two‐step and one‐step measurement with VIENA was 1.01%, while CCC was 0.98. Neither initial ventricular volume nor ventricular volume change affected performance of the method. Discussion: VIENA has good accuracy and good precision across four image types. VIENA therefore provides a useful fully automated method for measuring ventricular volume change in large datasets. Conclusion: VIENA is a robust, accurate, and precise method for measuring ventricular volume change. Hum Brain Mapp 35:1101–1110, 2014. © 2013 Wiley Periodicals, Inc.
System Integration of NFC Ticketing into an Existing Public Transport Infrastructure

A key application of Near Field Communication (NFC) can be found in the field of Electronic Fare Management. It can radically change existing systems of isolated applications in public transport by providing new approaches for a national or international inter operable fare management. In this paper a scenario for the integration of an electronic ticketing system into an existing public transport system based on NFC is introduced. The main focus is its realisation in accordance with the VDV Core Application. Electronic fare management systems consist of sophisticated structures and processes. Therefore, at the current stage of development only a selected subset of features which is essential for prototypical implementation is presented in this paper. First, the technology, electronic ticketing and previous field trials in this application area are introduced. Next, a set of relevant use cases is outlined and the existing system architecture is presented as basis for the description of the chosen system integration scenario. Finally the adopted and newly implemented system components and their interfaces are described in detail before concluding with faced challenges and some future prospects.