Miracle-dz/UniFAR-FTUs huggingface dataset:
Liquid chromatographic/mass spectrometric detection of the 3-(3-hydroxyalkanoyloxy) alkanoic acid precursors of rhamnolipids in Pseudomonas aeruginosa cultures.

A series of pseudomolecular and fragment ions attributed to 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs) were detected by liquid chromatography/mass spectrometry among the rhamnolipids observed in a Pseudomonas aeruginosa strain 57RP supernatant. The fragmentation mechanism leading to the formation of the fragment ions was determined by a deuterium exchange experiment and by using a standard HAA mixture obtained from the mild acidic hydrolysis of rhamnolipids of known composition. The structure and the response factor of these free HAAs were determined. The HAAs relative composition differs between free HAAs and those present in rhamnolipids, the former being enriched in lower molecular mass congeners and depleted in the heavier ones. Within an isomeric pair, the isomer with the shortest 3-hydroxyalkaloyl residue at the hydroxyl end was more abundant than the one with the heavier 3-hydroxyalkaloyl acid at this position, and the ratios of their relative abundances were similar for free HAAs and those in rhamnolipids. Experiments with deuterium-labeled rhamnolipids demonstrated that free HAAs are part of a pool used for rhamnolipid biosynthesis and are not rhamnolipid degradation products.
Multilabel Classification Rule with Performance Constraints

A formulation for multilabel and performance constraints classification problems is presented within the framework of statistical decision theory. The definition of the problem takes into account three concerns. The first is the cost function which defines the criterion to minimize; the second is the decision options which are defined by the admissible assignment classes or subsets of classes and the third one is the constraints of performance. Assuming that the conditional probability density functions are known, the classification rule that is solution of the stated problem is expounded. Two examples are provided to illustrate the formulation and the decision rule obtained
Onset of dyspraxia in aging persons with Down syndrome: Longitudinal studies

The slow and insidious development of progressive dyspraxia is recognised as an early symptom of Alzheimer disease (AD) among aging persons from the general population. However, very little is known about the age of onset and development of these AD-associated movement-related disorders among aging persons with Down syndrome (DS). This report provides a brief description of the development and standardisation of a 62-item dyspraxia scale adapted for adults with Down syndrome. It also provides a progress report utilising the scale in a 3.5-year longitudinal study designed to test the hypothesis that older individuals with DS would provide evidence for the onset and progression of dyspraxia when compared with younger adults with DS. Persons with Down syndrome who were 40 years of age and older at the start showed statistically significant deterioration on some of the dyspraxia measures suggestive of “preclinical” signs of dementia of the Alzheimer type (DAT). An old group, with normal dyspraxia scores at a .
Is ''pop-up'' messaging in online slot machine gambling effective as a responsible gambling strategy?

Certain gambling operators now provide social responsibility tools to help players gamble more responsibly. One such innovation is the use of pop-up messages that aim to give feedback to the players about the time and money they have thus far spent gambling. Most studies of this innovation have been conducted in laboratory settings, and although controlled studies are indeed more reliable than real-world studies, the non-ecological validity of laboratory studies is still an issue. This study investigated the effects of a slot machine pop-up message in a real gambling environment by comparing the behavioural tracking data of two representative random samples of 400,000 gambling sessions before and after the pop-up message was introduced. The study comprised approximately 200,000 gamblers. The results indicated that, following the viewing of a pop-up message after 1000 consecutive gambles on an online slot machine game, nine times more gamblers ceased their gambling session than did those gamblers who had not viewed the message. The data suggest that pop-up messages can influence a small number of gamblers to cease their playing session, and that pop-ups appear to be another potentially helpful social responsibility tool in reducing excessive play within session.
Advanced Rotor Fault Diagnosis for Medium-Voltage Induction Motors Via Continuous Transforms

A number of field case studies for rotor fault diagnosis on medium-voltage induction motors operating in a petrochemical plant are presented in this paper. The methodology employed is based on analyzing the induction motor startup current with advanced signal processing tools (continuous transforms) that enable a capture of a “complete picture” of the rotor condition. Indeed, unlike the classical tools that often rely on the detection of few fault frequencies, these new tools allow extraction of the evolution of a wide range of fault components during the startup transient and steady-state evolutions, which enables improved reliability. This is crucial in medium-high-voltage motors, where a false diagnosis may result in significant expense due to inspection, repair, or forced outage. An additional contribution of the study is its immunity to external voltage supply disturbances, which introduce components that are not related to the failure and which are difficult to detect with classical tools. The results of this study prove how the advanced continuous tools enable an improved visualization of the fault components, distinguishing them from the other components that are not linked to the failure.
Block of Kcnk3 by Protons

KCNK subunits have two pore-forming P domains and four predicted transmembrane segments. To assess the number of subunits in each pore, we studied external proton block of Kcnk3, a subunit prominent in rodent heart and brain. Consistent with a pore-blocking mechanism, inhibition was dependent on voltage, potassium concentration, and a histidine in the first P domain (P1H). Thus, at pH 6.8 with 20 mm potassium half the current passed by P1H channels was blocked (apparently via two sites ∼10% into the electrical field) whereas channels with an asparagine substitution (P1N) were fully active. Furthermore, pore blockade by barium was sensitive to pH in P1H but not P1N channels. Although linking two Kcnk3 subunits in tandem to produce P1H-P1H and P1N-P1N channels bearing four P domains did not alter these attributes, the mixed tandems P1H-P1N and P1N-P1H were half-blocked at pH ∼6.4, apparently via a single site. This implicates a dimeric structure for Kcnk3 channels with two (and only two) P1 domains in each pore and argues that P2 domains also contribute to pore formation.
Role of Impedance Audiometry in Evaluation of Eustachian Tube Function and Its Correlation with Tympanoplasty Surgery Outcome: Our Experience

Background: Normal Eustachian tube function is one of the important factor required for successful tympanoplasty outcome. A clinically useful test for assessment of Eustachian tube function is still lacking. Aims And Objectives: To evaluate Eustachian tube function by impedance audiometry and compare the outcome of surgery with respect to graft take up in normal and abnormal Eustachian tube function. Study design Prospective study. Materials and Methods: Study includes 60 patients diagnosed as chronic suppurative otitis media, mucosal type in quiescent or inactive stage. All patients were evaluated with detailed history, ENT examination and radiological investigations. Eustachian tube function was assessed with impedance audiometry. Preoperative Eustachian tube function results were compared with postoperative surgical outcome and results were analyzed. Results: On impedance audiometry 65% patients had normal Eustachian tube function and 35% had impaired function. Overall graft take up rate was 91.66% and failure rate was 8.34%. Tympanoplasty was successful in 94.87% patients with normal Eustachian tube function and in 85.71% with impaired function and difference was not statistically significant (P >0.05). Hearing improvement in terms of air-bone gap closure (AB gap < 20db) was more in patients with normal Eustachian tube function (61.66%). Conclusion: Impedance audiometry is a simple and easy method to assess Eustachian tube function. It not merely assess the anatomical patency but also the physiological function of Eustachian tube. Outcome of Tympanoplasty would be a success in normal Eustachian tube function but impaired tubal function should not be the ground for refusal of surgery.
Generation of scalable summaries based on iterative GoP ranking

Video skims and image storyboards are two widely used abstractions for representing the essence of a video sequence, crucial for effective browsing and retrieval applications. In this paper we propose a flexible approach to find a reasonable balance between the semantic coverage and naturalness of the generated summaries, targeting a wide range of summarization ratios. The result of the algorithm is a scalable representation of the information required for summarization (a ranked list of GoPs) with a number of advantages in terms of efficient generation and potential applications.
Design and Implementation of Embedded TCM System Based on Android Platform

TCM (Traditional Chinese Medicine) prescriptions are too complicated and numerous for most learners to remember, so the dialectic classification and the choice of TCM prescriptions are usually out of joint in clinic and teaching nowadays. The embedded system, described in this paper, is designed as a TCM prescription information system based on Android platform, in which the emphasis is placed on the standardization of TCM information and the promotion of the TCM traditional spreading approach. And it is implemented with the techniques regarding the Android platform and embedded database, to provide functions such as retrieval of recipes, herbs and syndromes, assisted prescribing and TCM information synchronization in Android device. Moreover, the system has established the integrated relationship of TCM entities relying on our TCM research to overcome the above obstacle in TCM development.
Ultralow-Leakage AlGaN/GaN High Electron Mobility Transistors on Si With Non-Alloyed Regrown Ohmic Contacts

Without employing gate dielectrics, AlGaN/GaN high-electron mobility transistors (HEMTs) on Si with non-alloyed regrown ohmic contacts exhibit record-low leakage currents ~10-12 A/mm, high ON/OFF current ratios 1011. Compared with HEMTs with conventional alloyed ohmic contacts, HEMTs with non-alloyed contacts show a reduction of 106 in leakage current, a steeper subthreshold slope, and 50% improvement in breakdown voltage. These observations indicate that avoiding high-temperature alloyed ohmic processes can lead to improved device performance.
Genetic diversity in Arabidopsis thaliana L. Heynh. investigated by cleaved amplified polymorphic sequence (CAPS) and inter‐simple sequence repeat (ISSR) markers

In this study, we investigated genetic diversity among 37 accessions in Arabidopsis thaliana from Eurasia, North Africa and North America using morphological traits and two polymerase chain reaction (PCR)‐based marker systems: cleaved amplified polymorphic sequences (CAPS) and inter‐simple sequence repeats (ISSR). Cluster analysis based on genetic similarities calculated from CAPS data grouped the accessions roughly according to their geographical origin: one large group contained accessions from Western, Northern and Southern Europe as well as North Africa, a second group consisted of Eastern European and Asian continental accessions. North American accessions were interspersed into these groups. Contrary to the CAPS analysis, the dendrogram obtained from the ISSR data did not reflect the geographical origin of the accessions, and the calculated genetic distances did not match the CAPS results. This could be attributable to an uneven genomic distribution of ISSR markers as substantiated by a database search for ISSR binding sites in A. thaliana genomic DNA sequence files, or to the ISSR’s different mode of evolution. We recommend CAPS markers for diversity analysis in A. thaliana because a careful selection of markers can ascertain an even representation of the entire genome.
The Role of Religious Coping in Adjustment to Prostate Cancer

This study explored the role of religious coping in men's long-term adjustment to prostate cancer. Thirty-four men with prostate cancer completed questionnaires on demographic and illness factors, religious and general coping, and physical, social, and emotional functioning. Results showed that religious coping was related to poorer role, social, and emotional functioning for these cancer survivors. In contrast, religious coping was related to positive aspects of cognitive appraisal and to both active and avoidance forms of general coping. Notably, religious coping may be used to help cancer survivers “block out” their everyday experience of the prostate cancer and its related complications. Such results suggest that religious coping functions in a complex manner within the context of long-term prostate cancer survival.
Riikka Susitaival and Samuli Aalto . Analyzing the file availability and download time in a P 2 P file sharing system

In this paper we study the performance of P2P file sharing systems. We are especially interested in how the division of the file into chunks as well as the used chunk selection policy influence on two performance metrics, named the availability and mean download time of the file. We propose a detailed Markov chain model that allows estimating the mean lifetime of the system and comparing different chunk selection policies exactly. In addition, we use simulations to evaluate more complex systems. Results suggest that splitting the file from one chunk into two chunks improves the performance of the system significantly. Meanwhile, the superiority of a chunk selection policy depends on many factors, such as the arrival rate of the new downloaders and service time distribution.
Specific and global coagulation assays in the diagnosis of discrepant mild hemophilia A

The activity of the factor VIII coagulation protein can be measured by three methods: a one or two-stage clotting assay and a chromogenic assay. The factor VIII activity of most individuals with mild hemophilia A is the same regardless of which method is employed. However, approximately 30% of patients show marked discrepancies in factor VIII activity measured with the different methods. The objective of this study was to investigate the incidence of assay discrepancy in our center, assess the impact of alternative reagents on factor VIII activity assays and determine the usefulness of global assays of hemostasis in mild hemophilia A. Factor VIII activity was measured in 84 individuals with mild hemophilia A using different reagents. Assay discrepancy was defined as a two-fold or greater difference between the results of the one-stage and two-stage clotting assays. Rotational thromboelastometry and calibrated automated thrombography were performed. Assay discrepancy was observed in 31% of individuals; 12% with lower activity in the two-stage assay and 19% with lower activity in the one-stage assay. The phenotype could not always be predicted from the individual’s genotype. Chromogenic assays were shown to be a suitable alternative to the two-stage clotting assay. Thromboelastometry was found to have poor sensitivity in hemophilia. Calibrated automated thrombography supported the results obtained by the two-stage and chromogenic assays. The current international guidelines do not define the type of assay to be used in the diagnosis of mild hemophilia A and some patients could be misclassified as normal. In our study, 4% of patients would not have been diagnosed on the basis of the one-stage factor VIII assay. Laboratories should use both one stage and chromogenic (or two-stage) assays in the diagnosis of patients with possible hemophilia A.
Effects of continuous estrogen and estrogen-progestin replacement regimens on cardiovascular risk markers in postmenopausal women.

OBJECTIVE To evaluate the influence of 2 continuous combined estrogen-progestin replacement products, compared with unopposed estrogen and placebo, on cardiovascular risk markers in postmenopausal women in a randomized, double-blind, placebo-controlled trial. METHODS Two hundred seventy healthy postmenopausal women were randomly assigned to 1 of 4 treatment groups: placebo, unopposed 17-beta estradiol (1 mg), 1 mg of 17-beta estradiol with 0.25 mg of norethindrone acetate, or 1 mg of 17-beta estradiol with 0.5 mg of norethindrone acetate. The primary outcome variable was change from baseline in low-density lipoprotein cholesterol concentration. Additional outcome variables included changes in other serum lipid levels, hemostatic variables, and indicators of carbohydrate metabolism. RESULTS The low-density lipoprotein cholesterol level was reduced to a similar degree in all groups receiving active treatment (10%-14% from baseline; P =.001 for 17-beta estradiol with 0.5 mg of norethindrone acetate, P =.004 for 17-beta estradiol with 0.25 mg of norethindrone acetate, and P =. 001 for 1 mg of 17-beta estradiol vs placebo). Compared with unopposed 17-beta estradiol, 17-beta estradiol with 0.5 mg of norethindrone acetate enhanced the reductions in total cholesterol and apolipoprotein B levels (P<.01 vs 17-beta estradiol). 17-beta Estradiol plus norethindrone blunted or reversed the increases in levels of high-density lipoprotein cholesterol, apolipoprotein A-I, and triglycerides produced by 17-beta estradiol alone. Effects of 17-beta estradiol plus norethindrone on hemostatic variables were similar to those of 17-beta estradiol except for factor VII activity, which was significantly reduced with 17-beta estradiol combined with 0.25 mg (P<.01) and 0.5 mg (P<.05) of norethindrone acetate. 17-beta Estradiol plus norethindrone appeared to blunt reductions in C-peptide and insulin levels produced by unopposed 17-beta estradiol but did not elevate these values compared with placebo. CONCLUSIONS 17-beta Estradiol plus norethindrone produced favorable changes in most cardiovascular risk markers evaluated and has a profile distinct from that of unopposed 17-beta estradiol. The impact of these differences on cardiovascular events warrants investigation. Arch Intern Med. 2000;160:3315-3325.
Emotional Schemas, Psychological Flexibility, and Anxiety: The Role of Flexible Response Patterns to Anxious Arousal

Recently, a number of cognitive behavioral therapy (CBT) process accounts of anxiety disorders have emerged that go beyond an emphasis on cognitive reappraisal or habituation (Barlow, Allen, & Choate, 2004; Hayes, Strosahl, & Wilson, 2011; Leahy, Tirch, & Napolitano, 2011; Mennin, Turk, Heimberg, & Carmin, 2005). Emotional Schema Theory (Leahy, 2002; Leahy, Tirch, & Napolitano, 2011) which is the conceptual framework supporting Emotional Schema Therapy (EST) and the Psychological Flexibility Model, which is the theoretical basis of Acceptance and Commitment Therapy (ACT; Hayes, Strosahl; & Wilson, 2011), are two such models. The current, cross-sectional study explores the relationship of emotional schemas, psychological flexibility, and mindfulness in persons with elevated anxiety as measured by the BAI (Beck & Steer, 1993) and the MCMI-III (Millon, 1997). Emotional schemas were assessed using the Leahy Emotional Schema Scale (LESS; Leahy, 2002). Psychological flexibility was measured by the Acceptance an.
On-line junction temperature measurement of IGBTs based on temperature sensitive electrical parameters

This paper deals with a method to derive the junction temperature of an IGBT while the device is in operation. In order to achieve this the gate-emitter voltage, the collector current and the collector-emitter voltage are digitized on the driver board. Due to the fact that material parameters vary with temperature, the waveforms of the switching transients vary with temperature, too. Thus, there is a correlation between the temperature and the switching waveforms. Evaluating temperature sensitive electrical parameters (TSEP), the working temperature of the device can be estimated.
Retroviral Insertions in Evi12, a Novel Common Virus Integration Site Upstream of Tra1/Grp94, Frequently Coincide with Insertions in the Gene Encoding the Peripheral Cannabinoid Receptor Cnr2

ABSTRACT The common virus integration site (VIS) Evi11 was recently identified within the gene encoding the hematopoietic G-protein-coupled peripheral cannabinoid receptor Cnr2(also referred to as Cb2). Here we show thatCnr2 is a frequent target (12%) for insertion of Cas-Br-M murine leukemia virus (MuLV) in primary tumors in NIH/Swiss mice. Multiple provirus insertions in Evi11 were cloned and shown to be located within the 3′ untranslated region of the candidate proto-oncogene Cnr2. These results suggest that proviral insertion in the Cnr2 gene is an important step in Cas-Br-M MuLV-induced leukemogenesis in NIH/Swiss mice. To isolateEvi11/Cnr2 collaborating proto-oncogenes, we searched for novel common VISs in the Cas-Br-M MuLV-induced primary tumors and identified a novel frequent common VIS, Evi12 (14%). Interestingly, 54% of the Evi11/Cnr2-rearranged primary tumors contained insertions in Evi12 as well, which suggests cooperative action of the target genes in these two common VISs in leukemogenesis. By interspecific backcross analysis it was shown that Evi12 resides on mouse chromosome 10 in a region that shares homology with human chromosomes 12q and 19p. Sequence analysis demonstrated that Evi12 is located upstream of the gene encoding the molecular chaperone Tra1/Grp94, which was previously mapped to mouse chromosome 10 and human chromosome 12q22–24. Thus, Tra1/Grp94 is a candidate target gene for retroviral activation or inactivation in Evi12. However, Northern and Western blot analyses did not provide evidence that proviral insertion had altered the expression ofTra1/Grp94. Additional studies are required to determine whether Tra1/Grp94 or another candidate proto-oncogene inEvi12 is involved in leukemogenesis.
Solving problems in genetics

A teaching unit on genetics and human inheritance using problem‐solving methodology was undertaken with fourth‐level Spanish Secondary Education students (15 year olds). The goal was to study certain aspects of the students’ learning process (concepts, procedures and attitude) when using this methodology in the school environment. The change experienced by students in the process of problem‐solving is discussed: the analysis of the problem, the formulation of hypothesis, the design of a solution, the putting into practice of that strategy and the analysis of results. As genetics is one of the few biology topics best addressed through problem‐solving, and problem‐solving traditionally has been treated in Spain as that of closed problems, it is important that we have shown that working with genuine problematic situations allows students to successfully solve closed problems without prior training.