Miracle-dz/UniFAR-FTUs huggingface dataset:
Simple, low‐cost multipurpose RF coil for MR microscopy at 9.4 T

An inductively coupled RF coil is introduced for high‐resolution MR studies at 9.4 T. The coil offers the flexibility of use as an implantable coil for local imaging inside the body or as a surface coil for imaging below complex surfaces. Successful operation of this coil at strong magnetic field requires special design considerations. In this note, implementation issues of the coil are discussed in detail and practical solutions to overcome some of these difficulties are offered. Imaging performance of the coil and its versatility are demonstrated with images acquired from rat spinal cord when the coil is implanted, and mouse spine and brain when the coil is placed on the surface. Magn Reson Med 52:937–940, 2004. © 2004 Wiley‐Liss, Inc.
Unpacking insight: How consumers are qualified by advertising agencies

By describing how consumers are qualified and mobilised in advertising agencies, this paper aims to contribute to this increasing body of literature that explores ordinary marketing and advertising practices, knowledge and devices. This is done by unpacking and analysing a particular aspect of routine advertising work, which is the production and circulation of insights about consumers in advertising agencies. We argue that producing insight involves performing a particular type of qualification of the consumer that relates to two specific processes. Firstly, we describe these practices in terms of an a extensive process of mediation that involves the deployment of progressive definitions of products and consumers that pass by different actors in the agency and through which production and consumption are connected in the very local and specific space of the advertising agency. Secondly, we argue that this process of mediation goes together with a process of ‘purification’ that involves performing a specific version of the consumer aligned with creative advertising work. Furthermore, we describe how this process involves considering some specific consumer qualities and descriptions (mostly interpretations about possible connections between goods and consumers) and leaving others asides. We identify this last operation as a particular type of cultural calculation. This argument is empirically supported by evidence collected from 40 interviews with advertising professionals and ethnographic fieldwork carried out at eight advertising agencies based in Santiago, Chile.
Bounded Game-Theoretic Semantics for Modal Mu-Calculus and Some Variants

We introduce a new game-theoretic semantics (GTS) for the modal mu-calculus. Our so-called bounded GTS replaces parity games with alternative evaluation games where only finite paths arise; infinite paths are not needed even when the considered transition system is infinite. The novel games offer alternative approaches to various constructions in the framework of the mu-calculus. For example, they have already been successfully used as a basis for an approach leading to a natural formula size game for the logic. While our main focus is introducing the new GTS, we also consider some applications to demonstrate its uses. For example, we consider a natural model transformation procedure that reduces model checking games to checking a single, fixed formula in the constructed models, and we also use the GTS to identify new alternative variants of the mu-calculus with PTime model checking.
Functional analysis of the tdcABC promoter of Escherichia coli: roles of TdcA and TdcR

The efficient expression of the tdc operon of Escherichia coli requires the products of two regulatory genes, tdcR and tdcA. We have identified the transcription site of tdcR by primer extension mapping and established the translation start site of TdcR by mutational analysis of its reading frame. In a tdcR tdcABC deletion strain, tdcR+ promoted high-level LacZ expression from a lambda tdcAB-lacZ lysogen and mutations introduced in tdcR resulted in a greater than sixfold decrease in LacZ level. In-frame deletions of tdcA also reduced LacZ expression, and chromosomal and plasmid-borne tdcA+ increased the LacZ level in tdcA mutant lysogens. Interestingly, multicopy tdcA+ plasmids introduced into tdcR mutant strains completely restored tdc expression. In separate experiments we found that mutations in the tdc promoter DNA around positions -70, -140, and -175 greatly reduced tdc expression relative to that for the wild-type promoter and the tdcP mutation around -175 prevented multicopy tdcA+ from rescuing tdcR mutants. Furthermore, competition experiments revealed that a wild-type promoter fragment encompassing the -175 region cloned into a plasmid reduced tdc expression by titrating TdcA in vivo, and this effect was reversed with excess TdcA. These results suggest that in tdcR+ cells TdcR interacts with tdcP and/or TdcA to enhance tdc transcription whereas in tdcR mutant cells a new tdcP-TdcA complex around -175 in the native promoter bypasses the requirement for TdcR. On the basis of the accumulated data summarized here and elsewhere we propose that multiple transcription factors enhance tdc operon expression by bending and looping of the promoter DNA to form an active transcription complex.
In Situ Detection of Integrin Ligands

Integrins are cell surface receptors for cell adhesion. Integrin‐mediated cell adhesion regulates various cellular processes, including cell survival, migration, proliferation, and differentiation. In vivo, ligands for integrins are immobilized within extracellular matrices, insoluble sheet‐like or fibrous supramolecular complexes that associate with or surround cells. To better understand the molecular basis of integrin‐mediated regulation of cellular behavior in vivo, it is of critical importance to collect information regarding the activities as well as spatial distributions of integrin ligands in situ. This unit describes a protocol for detecting the spatial distribution of the complement of integrin ligands in situ by overlaying soluble recombinant integrins. © 2014 by John Wiley & Sons, Inc.
Modeling neural population data

A fundamental challenge in Neuroscience is to infer the emergent properties of networks of neurons. Our current understanding of neural processing is largely based on the response properties of single cells, but techniques to simultaneously record action potentials from populations of neurons are rapidly advancing. This provides new challenges for probabilistic models to characterize networks and to understand their connectivity as well as computational function. We present an overview of statistical models to describe the activity of simultaneously recorded neurons. These methods allow us to interpret the network activity in terms of underlying circuit structure and give insight into functional connectivity.
Structural characterization of virion proteins and genomic RNA of human parainfluenza virus 3

The virion proteins and genomic RNA of human parainfluenza virus 3 have been characterized. The virion contains seven major and two minor proteins. Three proteins of 195 X 10(3) molecular weight (195K), 87K, and 67K are associated with the nucleocapsid of the virion and have been designated L, P, and NP, respectively. Three proteins can be labeled with [14C]glucosamine and have molecular weights of 69K, 60K, and 46K. We have designated these proteins as HN, F0, and F1, respectively. HN protein has interchain disulfide bonds, but does not participate in disulfide bonding to form homomultimeric forms. F1 appears to be derived from a complex, F1,2, that has an electrophoretic mobility similar to that of F0 under nonreducing conditions. A protein of 35K is associated with the envelope components of the virion and aggregates under low-salt conditions; this protein has been designated M. The genome of human parainfluenza virus 3 is a linear RNA molecule with a molecular weight of approximately 4.6 X 10(6).
Power Synchronization Control for Grid-Connected Current-Source Inverter-Based Photovoltaic Systems

Current-source converters (CSCs) have a promising potential to interface the large-scale photovoltaic (PV) generators to electric grids. In order to overcome several drawbacks associated with the conventional vector-controlled CSC-based PV systems, this paper presents for the first time, the power synchronization control (PSC) scheme for the single-stage grid-connected CSC-based PV generator. A detailed small-signal model for the complete CSC-based PV system with the PSC strategy is developed to investigate the system stability, characterize the converter interactions with the electric grid, and design the controller parameters. The PSC of the CSC-based PV system reflects a stable performance under different operating conditions and following sudden variations in the insolation level. Due to the inherent synchronizing feature of the PSC, the phase-locked loop is eliminated and, hence, the integration to the very weak grids is seamlessly achieved. Although there is no direct control on the injected ac current to the grid, the inherent self-current-limiting feature of the CSC through the modulation of the regulated dc-choke current offers an overcurrent protection. Detailed nonlinear time-domain simulations results validate the theoretical analysis and show the effectiveness of the PSC strategy.
Reduction of vasogenic edema and infarction by MK-801 in rats after temporary focal cerebral ischemia.

Blood-brain barrier permeability alteration, vasogenic brain edema, and infarction, which are more extensive after 3 hours of temporary middle cerebral artery occlusion (MCAO) and 3 hours of reperfusion than after 6 hours of permanent MCAO, develop in rats after prolonged focal cerebral ischemia. Protective effects of excitatory amino acid receptor antagonists have been previously demonstrated after temporary global ischemia and permanent focal ischemia in rats. The purpose of this study was to evaluate the effectiveness of MK-801, a noncompetitive N-methyl-D-aspartate receptor antagonist, in temporary middle cerebral artery occlusion in rats maintained at physiological levels of brain temperature. Rats were anesthetized with chloral hydrate (350 mg/kg, intraperitoneally). The MCAO of rats was occluded by cannulation with a nylon suture for 3 hours, followed by 3 hours of reperfusion accomplished by withdrawing the suture. MK-801 (1 mg/kg, intravenously) or saline (S) was injected immediately before the onset of MCAO. Water content (MK-801, n = 6; S, n = 6), Evans blue dye extravasation (MK-801, n = 6; S, n = 6), infarct volume (MK-801, n = 10; S, n = 10), histology (MK-801, n = 6; S, n = 6), and neurological deficit (MK-801, n = 15; S, n = 18) were measured at the end of 3 hours of reperfusion. Brain temperature was monitored during the experiment. The infarction area (measured by 2, 3, 5-triphenyltetrazolium chloride staining) was reduced (P < 0.001) in the MK-801-treated rats, as was the infarct volume and the severity of neuronal damage (P < 0.01). (ABSTRACT TRUNCATED AT 250 WORDS)
Current Source Modular Multilevel Converter: Detailed Analysis and STATCOM Application

Multilevel current source converters are a smart choice for the high-power applications with low-voltage and high-current requirements. This paper presents the detailed analysis of a current source modular multilevel converter (CSMMC) and its application as a STATCOM. Various properties of CSMMC are compared with the voltage-source modular multilevel converter (VSMMC). The submodule inductance selection method and a sorting-based algorithm for inductor current balancing are proposed for CSMMC. The validity of the inductance selection method and the inductor current balancing algorithm are confirmed by the simulation of a three-phase CSMMC using PSCAD/EMTDC. The carrier phase-shifted sinusoidal pulsewidth modulation is used as the switching technique. The performance of a converter is analyzed with interleaved and noninterleaved carrier signals for the upper and lower arms. Results show that CSMMCs and corresponding VSMMCs have analogous features. Hence, the development on VSMMCs can be used for reference to CSMMCs and vice-versa. Furthermore, the control designs for CSMMC-based STATCOM are presented and verified using simulations. The obtained results of the proposed STATCOM show that the steady-state and transient performance of the system are quite satisfactory. This makes the CSMMC suitable for HVDC and flexible ac transmission systems applications.
Investigation of known genetic risk factors for primary open angle glaucoma in two populations of African ancestry.

PURPOSE Multiple genes have been associated with primary open angle glaucoma (POAG) in Caucasian populations. We now examine the association of these loci in populations of African ancestry, populations at particularly high risk for POAG. METHODS We genotyped DNA samples from two populations: African American (1150 cases and 999 controls) and those from Ghana, West Africa (483 cases and 593 controls). Our analysis included 57 single nucleotide polymorphisms (SNPs) in five loci previously associated with POAG at the genome-wide level, including CDKN2B-AS1, TMCO1, CAV1/CAV2, chromosome 8q22 intergenic region, and SIX1/SIX6. We evaluated association in the full datasets, as well as subgroups with normal pressure glaucoma (NPG, maximum IOP ≤21 mm Hg) and high pressure glaucoma (HPG, IOP >21 mm Hg). RESULTS In African Americans, we identified an association of rs10120688 in the CDNK2B-AS1 region with POAG (P = 0.0020). Several other SNPs were nominally associated, but did not survive correction for multiple testing. In the subgroup analyses, significant associations were identified for rs10965245 (P = 0.0005) in the CDKN2B-AS1 region with HPG and rs11849906 in the SIX1/SIX6 region with NPG (P = 0.006). No significant association was identified with any loci in the Ghanaian samples. CONCLUSIONS POAG genetic susceptibility alleles associated in Caucasians appear to play a greatly reduced role in populations of African ancestry. Thus, the major genetic components of POAG of African origin remain to be identified. This finding underscores the critical need to pursue large-scale genome-wide association studies in this understudied, yet disproportionately affected population.
Comparison of different threshold values r for approximate entropy: application to investigate the heart rate variability between heart failure and healthy control groups

Approximate entropy (ApEn) is widely accepted as a complexity measure of the heart rate variability (HRV) signal, but selecting the criteria for the threshold value r is controversial. This paper aims to verify whether Chon's method of forecasting the rmax is an appropriate one for the HRV signal. The standard limb lead ECG signals of 120 subjects were recorded for 10 min in a supine position. The subjects were divided into two groups: the heart failure (22 females and 38 males, median age 62.4 ± 12.6) and healthy control group (33 females and 27 males, median age 51.5 ± 16.9). Three types of ApEn were calculated: the ApEn0.2 using the recommended constant r = 0.2, the ApEnchon using Chon's method and the ApEnmax using the true rmax. A Wilcoxon rank sum test showed that the ApEn0.2 (p = 0.267) and the ApEnmax (p = 0.813) had no statistical differences between the two groups, while the ApEnchon (p = 0.040) had. We generated a synthetic database to study the effect of two influential factors (the signal length N and the ratio of short- and long-term variability sd1/sd2) on the empirical formula in Chon's method (Chon et al 2009 IEEE Eng. Med. Biol. Mag. 28 18–23). The results showed that the empirical formula proposed by Chon et al is a good method for analyzing the random signal, but not an appropriate tool for analyzing nonlinear signals, such as the logistic or HRV signals.
Text-based sentential stress prediction using continuous lexical embedding for Mandarin speech synthesis

Stress is an important parameter for prosody processing in speech synthesis. However, it is not easy to stress from text analysis due to the complicated information. In this paper, we explore the novel use of the continuous lexical embedding and bidirectional long short-term memory recurrent neural network (BLSTM) model into sentential stress prediction for Mandarin speech synthesis. We look at augmenting the baseline features with word representations that are derived from text, providing continuous embedding of the lexicon in a low-dimensional space. Although learned in an unsupervised fashion, such features capture semantic and syntactic properties that make them amenable for stress prediction. We deploy various embedding models on Mandarin sentential stress prediction, showing substantial gains (relative gain gains of approximately 7.4% in F1 score).
Role of transcription pausing in the control of the pyrE attenuator in Escherichia coli

Expression of the Escherichia coli pyrE gene is regulated by transcription attenuation in the intercistronic orfE–pyrE region and modulated by the distance between the transcribing RNA polymerase and the leading ribosome as a function of the supply of UTP and GTP. In this communication we show that pyrE expression is hyper‐repressed in vivo following addition of uracil in strains carrying the nusAcs10 mutation. This phenotype, previously seen in rpsL 1204 strains whose ribosomes are pseudodependent on streptomycin and work at suboptimal elongation rate, indicates that RNA polymerase escapes from the ribosomes in the pyrE attenuator region in the nusA mutant. In vitro transcription studies revealed that the build‐up of the full‐length attenuated orfE transcript occurred more slowly in the presence of the NusA protein than in its absence. Moreover, the NusA protein enhanced several transcription pauses through the orfE gene. These effects were more pronounced when low concentrations of either UTP or GTP were used than at low concentrations of either CTP or ATP. The results indicate that the NusA protein is required for proper regulation of pyrE gene expression and is involved, together with the NTP pools, in maintaining the coupling between transcription and translation in the pyrE attenuator region by inhibiting RNA chain elongation.
The Effect of Copper on Demineralization of Dental Enamel

Previous studies have concluded that copper might inhibit enamel demineralization in vitro. Our aim was to assess the effect of copper (Cu2+), with and without amine fluoride, on human dental enamel under cariogenic challenge in situ. In a double-blind randomized four-leg crossover trial, 14 individuals wore a removable appliance containing 2 enamel slabs, 1 containing an artificial caries lesion. During each leg, the appliance was exposed twice daily to one of the test solutions: 1.25 mM CuSO4, amine fluoride (250 ppm F), copper and amine fluoride combined, or a placebo (water). A cariogenic challenge was provided in all cases by 5 daily exposures to 10% sucrose. Slabs were assessed before and after 21 days’ exposure by Knoop microhardness and transverse microradiography. Significantly less demineralization was observed with Cu2+ and fluoride in combination than with fluoride treatment alone (p < 0.05), whereas copper alone had no significant protective effect.
Ontology-based service matching in cloud computing

This paper focuses on how to maximize accuracy of Cloud service discovery and give enough flexibility to Cloud customers to discover their best suited services from a range of Cloud providers. An ontology-based Cloud service discovery approach is proposed, which works based on modeling semantically enriched Cloud services, ontology reasoning and logic matchmaking. Cloud customers have different preferences for non-functional attributes, ranking discovered services according their preference can help select the most appropriate cloud service. Experimental results show that the discovered services not only meet customer's requirements in semantics but also satisfy QoS requirements given in the terms of SLA.
Digital Commons @ East Tennessee State University Digital Commons @ East Tennessee State University

Aging is associated with an increase in need for assistance. Preparation for future care (PFC) is related to improved coping ability as well as better mental and physical health outcomes among older adults. We examined the association of optimism with components of PFC among older adults. We also explored race differences in the relationship between optimism and PFC. In Study 1, multiple regression showed that optimism was positively related to concrete planning. In Study 2, optimism was related to gathering information. An exploratory analysis combining the samples yielded a race interaction: For Whites higher optimism, but for Blacks lower optimism was associated with more planning. High optimism may be a barrier to future planning in certain social and cultural contexts.
Endothelial cell-binding antibodies in patients with systemic lupus erythematosus

Abstract The implications of endothelial cell-binding IgG antibodies (EC IgG) in systemic lupus erythematosus (SLE) was evaluated by determining level of EC IgG in sera from 112 SLE patients. The serum EC IgG level was determined by the cyto-ELISA method using human umbilical vein endothelial cells (HUVEC), human microvascular endothelial cells (HMVEC), and aortic endothelial cells (HAEC) as antigens. The levels of EC IgG were significantly higher among patients with SLE than among healthy control subjects (P < 0.001), and 68% (76/112) of SLE patients were shown to be EC IgG-positive. In patients with active lupus nephritis, the level of EC IgG was statistically and significantly elevated compared with those without lupus nephritis (P < 0.05). Negative correlations between EC IgG level and levels of CH50, C3, and lymphocyte count were revealed (P < 0.05, P < 0.005, and P < 0.05, respectively). When clinical course was evaluated, the levels of EC IgG correlated with disease activity. Definitive correlations in antibody levels between HUVEC and HMVEC, and between HUVEC and HAEC were revealed (both P < 0.0001). The results of this study revealed that the EC IgG in patients with SLE was involved in the onset of clinical manifestation, especially in patients with active lupus nephritis.
Orientation Practices for Effective Distributed Learning Coursework: Students Speak Their Minds.

As an increasing number of graduate education courses are moved either online or into hybrid formats, instructors and administrators need to consider strategies for how to transition students into these new learning environments. This exploratory, qualitative study looked at one case study course and provides practical recommendations for stronger orientation programs. Colleges and universities have increasingly moved coursework and professional development programs from traditional classroom environments to online or hybrid forums in an effort to maximize potential participation and minimize costs. This drive for online learning has been well documented in a variety of forums, and has been estimated to be worth $23 billion per year to higher education (Wei, 2001). The primary challenges to moving courses into a technologically mediated forum have typically fallen into two categories: instructor and technology. Technological limitations have been dramatically reduced in recent years as commercial providers such as WebCT and Blackboard have begun to provide commercially available products marketed specifically to higher education. The dominant problem, documented by a number of scholars and practitioners, has been trying to get faculty members to participate in offering courses online or in hybrid formats (Pajo & Wallace, 2001; Lynch & Lynch, 2003). Faculty resistance might have initially been based on technology ability or apprehension (Crooks, Yang, & Duemer, 2003), and has most recently been discussed in the form of motivation and compensation. Many institutions initially provided financial incentives to faculty to develop courses that used an online component, and increasingly, colleges and universities are expecting faculty to make the transition to mediated