Microcircuits neuroscience to understand pathophysiology

The histological scale is the preferred level for a large number of anatomical, pathological, pharmacological, immunochemical, and molecular studies. However, neurophysiological studies are mostly performed at the cellular/molecular or systems levels. The former describes the biology of the nerve cells and their synapses considering the genetic and signaling paths that underlie neural function while the latter involves the localization of cerebral processes correlating neural activity with sensation, motor coding, emotions and cognition. All levels are necessary to build a complete picture of any organ more so in the case of the brain. But there is no easy way to connect the cellular/molecular and systems levels without intermediate stances [1]. Even though, with the use of high technological tools, more recent work claims to do so: from molecules to behavior. The result has been a large amount of data: several different molecules or cellular processes are being proposed as potential causes of the same behavior, disease or circuit malfunction [1]. One example is Parkinson’s disease (PD), where it is known that animal models with dopamine deprivation end up with several synaptic and neuronal dysfunctions in different brain nuclei and circuits, and although all of them claim to be correlated with the disease, none of them can be said to be the single most important disorder that causes it, except for dopamine deprivation itself. It is hard to connect all these disorders in a single unifying theory that explains PD signs and symptoms. In the same way it is common place to correlate synaptic plasticity of a particular connection with learning and memory of a whole animal. Since Sherrington, Brown, Hebb, Mouncastle and others, it has been posited that mammalian neurons do not work alone but do so in modular arrangements or microcircuits that iterate in the brain with certain variance. In trying to begin fill the gap between cellular and systems levels we thought that the histological scale deserves to be explored physiologically to try to understand how a neuronal microcircuit conformed by several neurons and synapses works. Methods to visualize and record the activity of dozens of cells simultaneously are available and are improving. Here, we used calcium imaging. What has been lacking is a way to describe and quantify the activity of a microcircuit in the same way that, at the cellular level, neurophysiologists characterize single neurons or synapses with measures such as input resistance, spike rate, time constant, quantal content, short-term dynamics, etc. In Pérez-Ortega et al. (2016) [2] we propose that measures taken from Graph Theory also called Network Theory can be used to describe and quantify the activity of a neural microcircuit, then a graphical visualization and quantitative description was devised by choosing a small set of parameters [2]: under control conditions neuronal ensembles consisted in clusters of neighboring cells connected with other clusters at distances that could not be explained by monosynaptic connections but needed the action of long axon neurons or interneurons. These “hub” Editorial: Neuroscience