Miracle-dz/UniFAR-FTUs huggingface dataset:
Metabolite Profiles of Lactic Acid Bacteria in Grass Silage

ABSTRACT The metabolite production of lactic acid bacteria (LAB) on silage was investigated. The aim was to compare the production of antifungal metabolites in silage with the production in liquid cultures previously studied in our laboratory. The following metabolites were found to be present at elevated concentrations in silos inoculated with LAB strains: 3-hydroxydecanoic acid, 2-hydroxy-4-methylpentanoic acid, benzoic acid, catechol, hydrocinnamic acid, salicylic acid, 3-phenyllactic acid, 4-hydroxybenzoic acid, (trans, trans)-3,4-dihydroxycyclohexane-1-carboxylic acid, p-hydrocoumaric acid, vanillic acid, azelaic acid, hydroferulic acid, p-coumaric acid, hydrocaffeic acid, ferulic acid, and caffeic acid. Among these metabolites, the antifungal compounds 3-phenyllactic acid and 3-hydroxydecanoic acid were previously isolated in our laboratory from liquid cultures of the same LAB strains by bioassay-guided fractionation. It was concluded that other metabolites, e.g., p-hydrocoumaric acid, hydroferulic acid, and p-coumaric acid, were released from the grass by the added LAB strains. The antifungal activities of the identified metabolites in 100 mM lactic acid were investigated. The MICs against Pichia anomala, Penicillium roqueforti, and Aspergillus fumigatus were determined, and 3-hydroxydecanoic acid showed the lowest MIC (0.1 mg ml−1 for two of the three test organisms).
Path‐planning techniques for the simulation of disassembly tasks

Purpose – This paper aims to develop path‐planning techniques that support a general selective disassembly planner in a virtual reality environment.Design/methodology/approach – The paper presents an automatic selective disassembly planning and two path‐planning techniques that support it. The first one is based on single translations, while the second is based on the generation of a random search tree. The methods used have been adapted and modified from available robotic path‐planning methods for their use in disassembly path planning.Findings – The paper finds that the proposed techniques are applicable to the automatic generation of disassembly sequences.Research limitations/implications – The paper provides an automatic tool that can be integrated in simulation software for the analysis and validation of disassembly operation.Practical implications – Maintenance operations have a great impact in the security and life expectancy of any product. This is especially true for some applications such as aer.
Wireless Channel Model of UWB for Underground Tunnels in Coal Mine

In this paper, the radio propagation of ultra wide band (UWB) in coal mine tunnels is analyzed according to channel path loss. The transmitted pulse and modulated method of UWB system are selected in allusion to its propagation characteristic. We present the method of improving time-variant finite impulse responding filter model to a trapped delay line channel model with specific center frequency and multipath resolution. The Improvement for a typical UWB pulse has been done, and the statistic characteristics of the modified channel model have been analyzed. The simulation results show that the multipath delay characteristics of the improved channel model well match the measured results of coal mine tunnels.
Analysis of the Compartmentalized Metabolome – A Validation of the Non-Aqueous Fractionation Technique

With the development of high-throughput metabolic technologies, a plethora of primary and secondary compounds have been detected in the plant cell. However, there are still major gaps in our understanding of the plant metabolome. This is especially true with regards to the compartmental localization of these identified metabolites. Non-aqueous fractionation (NAF) is a powerful technique for the determination of subcellular metabolite distributions in eukaryotic cells, and it has become the method of choice to analyze the distribution of a large number of metabolites concurrently. However, the NAF technique produces a continuous gradient of metabolite distributions, not discrete assignments. Resolution of these distributions requires computational analyses based on marker molecules to resolve compartmental localizations. In this article we focus on expanding the computational analysis of data derived from NAF. Along with an experimental workflow, we describe the critical steps in NAF experiments and how computational approaches can aid in assessing the quality and robustness of the derived data. For this, we have developed and provide a new version (v1.2) of the BestFit command line tool for calculation and evaluation of subcellular metabolite distributions. Furthermore, using both simulated and experimental data we show the influence on estimated subcellular distributions by modulating important parameters, such as the number of fractions taken or which marker molecule is selected. Finally, we discuss caveats and benefits of NAF analysis in the context of the compartmentalized metabolome.
A Novel Oncolytic Herpes Simplex Virus that Synergizes with Phosphoinositide 3-kinase/Akt Pathway Inhibitors to Target Glioblastoma Stem Cells

Purpose: To develop a new oncolytic herpes simplex virus (oHSV) for glioblastoma (GBM) therapy that will be effective in glioblastoma stem cells (GSC), an important and untargeted component of GBM. One approach to enhance oHSV efficacy is by combination with other therapeutic modalities. Experimental Design: MG18L, containing a US3 deletion and an inactivating LacZ insertion in UL39, was constructed for the treatment of brain tumors. Safety was evaluated after intracerebral injection in HSV-susceptible mice. The efficacy of MG18L in human GSCs and glioma cell lines in vitro was compared with other oHSVs, alone or in combination with phosphoinositide-3-kinase (PI3K)/Akt inhibitors (LY294002, triciribine, GDC-0941, and BEZ235). Cytotoxic interactions between MG18L and PI3K/Akt inhibitors were determined using Chou–Talalay analysis. In vivo efficacy studies were conducted using a clinically relevant mouse model of GSC-derived GBM. Results: MG18L was severely neuroattenuated in mice, replicated well in GSCs, and had anti-GBM activity in vivo. PI3K/Akt inhibitors displayed significant but variable antiproliferative activities in GSCs, whereas their combination with MG18L synergized in killing GSCs and glioma cell lines, but not human astrocytes, through enhanced induction of apoptosis. Importantly, synergy was independent of inhibitor sensitivity. In vivo, the combination of MG18L and LY294002 significantly prolonged survival of mice, as compared with either agent alone, achieving 50% long-term survival in GBM-bearing mice. Conclusions: This study establishes a novel therapeutic strategy: oHSV manipulation of critical oncogenic pathways to sensitize cancer cells to molecularly targeted drugs. MG18L is a promising agent for the treatment of GBM, being especially effective when combined with PI3K/Akt pathway–targeted agents. Clin Cancer Res; 17(11); 3686–96. ©2011 AACR.
Topic Level Disambiguation for Weak Queries

Despite limited success, today’s information retrieval (IR) systems are not intelligent or reliable. IR systems return poor search results when users formulate their information needs into incomplete or ambiguous queries (i.e., weak queries). Therefore, one of the main challenges in modern IR research is to provide consistent results across all queries by improving the performance on weak queries. However, existing IR approaches such as query expan sion are not overly effective because they make little effort to analyze and exploit the meanings of the queries. Furthermore, word sense disambiguation approaches, which rely on textual context, are ineffective against weak queries that are typically short. Motivated by the demand for a robust IR system that can consistently provide highly accurate results, the proposed study implemented a novel topic detection that leveraged both the lan guage model and structural knowledge of Wikipedia and systematically evaluated the effect of query disam biguation and topic-based retrieval approaches on TREC collections. The results not only confirm the effective ness of the proposed topic detection and topic-based retrieval approaches but also demonstrate that query dis ambiguation does not improve IR as expected.
Towards a personal robotics development platform: Rationale and design of an intrinsically safe personal robot

The most critical challenge for Personal Robotics is to manage the issue of human safety and yet provide the physical capability to perform useful work. This paper describes a novel concept for a mobile, 2-armed, 25-degree-of- freedom system with backdrivable joints, low mechanical impedance, and a 5 kg payload per arm. System identification, design safety calculations and performance evaluation studies of the first prototype are included, as well as plans for a future development.
Aripiprazole in children and adolescents with Tourette's disorder: an open-label safety and tolerability study.

OBJECTIVE The aim of this study was to conduct a prospective safety and tolerability study of aripiprazole for the treatment of tics in children and adolescents with Tourette's disorder (TD). METHOD Eleven subjects (10 males) with TD (age 9-19 years, mean 13.36, standard deviation [SD] 3.33) who did not respond or were unable to tolerate previous tic medication were treated with aripiprazole in an open-label, flexible-dosing study over 10 weeks. Tic severity was rated using the Yale Global Tic Severity Scale (YGTSS) and the Clinical Global Impressions Scale for tics (CGI-Tics) at baseline and at follow-up. RESULTS The mean (+/-SD) daily dose for aripiprazole was 4.5 +/- 3.0 mg. Mean (+/-SD) YGTSS Global Severity scores reduced from 61.82 +/- 13.49 at baseline to 33.73 +/- 15.18 at end point; mean YGTSS total tic scores reduced from 28.18 +/- 7.74 at baseline to 16.73 +/- 7.54 at end point. Mean (+/-SD) CGI-Tic severity scores reduced from 4.45 +/- 0.52 (moderate-marked) at baseline to 3.18 +/- 0.60 (mild) at end point. On the CGI-Tic improvement scale, 10 (91%) subjects achieved 1 ("very much improved") or 2 ("much improved") at end point. Most common adverse effects included appetite increase and weight gain in 5 subjects, mild extrapyramidal effects in 7 subjects, and headaches and tiredness/fatigue in 7 subjects; 1 subject experienced akathisia and muscle cramps. CONCLUSION Aripiprazole appears to be a safe and tolerable treatment in children and adolescents with TD that appears to reduce tics; it should be further investigated as a treatment option in controlled trials.
(Re)configuration based on model generation

Reconfiguration is an important activity for companies selling configurable products or services which have a long life time. However, identification of a set of required changes in a legacy configuration is a hard problem, since even small changes in the requirements might imply significant modifications. In this paper we show a solution based on answer set programming, which is a logic-based knowledge representation formalism well suited for a compact description of (re)configuration problems. Its applicability is demonstrated on simple abstractions of several real-world scenarios. The evaluation of our solution on a set of benchmark instances derived from commercial (re)configuration problems shows its practical applicability.
Determination of Lamotrigine in Pharmaceutical Preparations by Adsorptive Stripping Voltammetry Using Screen Printed Electrodes

This paper describes a procedure that has been optimized for the determination of lamotrigine by Differential Pulse Adsorptive Stripping Voltammetry (DPAdSV) using carbon screen-printed electrodes (CSPE) and mercury coated carbon screen-printed electrodes. Selection of the experimental parameters was made using experimental design methodology. The detection limit found was 5.0 × 10-6 M and 2.0 × 10-6 M for the non modified and Hg modified CSPE, respectively. In terms of reproducibility, the precision of the above mentioned methods was calculated in %RSD values at 9.83% for CSPE and 2.73% for Hg-CSPE. The Hg-coated CSPEs developed in this work were successfully applied in the determination of lamotrigine in pharmaceutical preparations.
Juegos de realidad alternativa para la educación cívica. Elementos de diseño: narrativa, juego y discurso.

Los juegos de realidad alternativa, “Alternate Reality Games” (ARG), son considerados una narrativa transmedia y han sido explorados en ambientes educativos dadas sus caracteristicas ludicas, narrativas, participativas e interactivas. Sin embargo, comunmente han sido creados con historias fijas que han despertado poco interes en los alumnos. Esta investigacion exploratoria consistio en solicitar a grupos de alumnos disenar su propio ARG. Para promover la reflexion sobre cambio social su propuesta debia estar relacionada con ciudadania, politica, justicia o cualquier otra situacion social o cultural de su interes. Los estudiantes investigaron y discutieron sobre estos temas para finalmente presentar una serie de propuestas que fueron analizadas basandose en narrativa, elementos de juego y discurso. Se encontraron varias ideas creativas para promover la disposicion a la participacion ciudadana. Con el fin de facilitar el uso y creacion de estos juegos estamos desarrollando una aplicacion cuyas caracteristicas son descritas en el articulo.
Immune Reactions following Cord Blood Transplantations in Adults

Cord blood transplantation (CBT) is an attractive alternative therapy in adult patients with advanced hematological malignancies in whom matched donors are unavailable. However, the risk of complications, especially infections, post-CBT increases the mortality rates in these patients. Although the incidence of acute and chronic graft versus host disease (GVHD) post-CBT is lower than that following bone marrow transplantation and peripheral blood stem cell transplantation (SCT), the additional immunosuppressive therapy required to treat it could increase the mortality in these patients. Further, chronic GVHD following CBT is milder and responds better to treatment than that occurring after bone marrow transplants. Unlike bone marrow transplantation, the onset of GVHD is a positive prognostic indicator of overall survival in patients receiving CBT, due to the graft versus malignancy (GVM) effect. This paper focuses on the immune reactions following CBT and aims to elucidate a management strategy for acute and chronic GVHD.
Aberrant Expression of Neuropilin-1 and -2 in Human Pancreatic Cancer Cells

Purpose: Neuropilin (Np)-1 and -2 are coreceptors for vascular endothelial growth factor (VEGF). This study was designed to assess their role in pancreatic ductal adenocarcinoma (PDAC). Experimental Design: We assessed Np-1 and Np-2 expression by real-time quantitative PCR in relation to the expression of VEGF ligands and receptors in pancreatic cancer cell lines and tissues. Results: ASPC-1, CAPAN-1, and PANC-1 pancreatic cancer cells and tumor-derived, laser-captured pancreatic cancer cells exhibited higher Np-1 and Np-2 mRNA levels than VEGF receptor-1, -2, or -3 mRNA levels. Transfection of Np-1 and Np-2 cDNAs in COS-7 cells, and treatment with tunicamycin revealed that both proteins were glycosylated. Both proteins were expressed in pancreatic cancer cell lines, in the PDAC samples, and in acinar cells adjacent to the cancer cells. The normal pancreas was devoid of Np-1 immunoreactivity, whereas Np-2 immunoreactivity was present in the endocrine islets and in some acinar cells, but not in ductal cells. Conclusions: The aberrant localization of Np-1 and Np-2 in the cancer cells in PDAC suggests that in addition to exerting proangiogenic effects, these coreceptors may contribute to novel autocrine-paracrine interactions in this malignancy.
Space-eecient Approximation Algorithms for Maxcut and Coloring Semideenite Programs

The essential part of the best known approximation algorithm for graph MAXCUT is approximately solving MAXCUT's semideenite relaxation. For a graph with n nodes and m edges, previous work on solving its semidef-inite relaxation for MAXCUT requires space ~ O(n 2). Under the assumption of exact arithmetic, we show how an approximate solution can be found in space O(m+n 1:5), where O(m) comes from the input; and therefore reduce the space required by the best known approximation algorithm for graph MAXCUT. Using the above space-eecient algorithm as a subroutine, we show an approximate solution for COLORING's semideenite relaxation can be found in space O(m) + ~ O(n 1:5). This reduces not only the space required by the best known approximation algorithm for graph COLORING, but also the space required by the only known polynomial-time algorithm for nding a maximum clique in a perfect graph.
Changes in functional connectivity of human MT/V5 with visual motion input

The neural basis of human mental function is characterized by interactions between brain regions. Temporal correlations in MR signals between areas may provide one method for investigating these interactions. This approach was used to examine functional connectivity in the motion processing system of the human brain. Correlations between MT/V5 and other brain regions were examined in a resting state (without visual stimulation) and in an active state produced by viewing moving concentric circles. A network of regions consistent with the known functional anatomy of visual processing was correlated with MT/V5 during rest. When subjects were viewing motion, a more limited network was correlated with MT/V5, suggesting MT/V5 was acting in concert with a smaller network specific to the task.
Cascaded H-bridge multilevel converter topology and three-phase balance control for large scale photovoltaic systems

The increase in the power levels of photovoltaic (PV) energy conversion systems has resulted in new large-scale grid connected configurations that have reached the megawatt level. This substantial increment in the power levels imposes new challenges to the grid interfacing converter, and therefore results in new opportunities to be explored. This work introduces a new medium voltage multilevel scheme based on a three-phase cascaded H-bridge (CHB) converter and multiple PV strings. The proposed configuration enables a large increase of the total power capacity of the PV system, while the introduction of a multilevel converter helps to improve both power quality and efficiency and medium voltage operation at the grid side. The main challenge of the proposed configuration is to handle the inherent power imbalances that occur not only between the different cells of one phase of the converter but also between the three phases. Simulation results of a 7-level CHB PV system are presented to validate the proposed topology and control method.
Spatio‐temporal response properties of local field potentials in the primate superior colliculus

Local field potentials (LFPs) are becoming increasingly popular in neurophysiological studies. However, to date, most of the knowledge about LFPs has been obtained from cortical recordings. Here, we recorded single unit activity (SUA) and LFPs simultaneously from the superior colliculus (SC) of behaving rhesus monkeys. The SC is a midbrain structure that plays a central role in the visual orienting response. Previous studies have characterised the visual and visuomotor response properties of SUA in the superficial layers of the SC and the intermediate layers of the SC, respectively. We found that the signal properties of SUA were well preserved in the LFPs recorded from the SC. The SUA and LFPs had similar spatial and temporal properties, and the response properties of LFPs differed across layers, i.e. purely visual in the superficial layers of the SC but showing significant motor responses in the intermediate layers of the SC. There were also differences between SUA and LFPs. LFPs showed a significant reversal of activity following the phasic visual response, suggesting that the neighboring neurons were suppressed. The results indicate that the LFP can be used as a reliable measure of the SC activity in lieu of SUA, and open up a new way to assess sensorimotor processing within the SC.
Using digital technologies to activate and evaluate emotions to creatively communicate ideas

Given the Digital Technologies rationale of the Australian Curriculum, it is critical that both teachers and students use design thinking to be creative and innovative producers of digital solutions and knowledge. Teachers and students are increasingly being required to self-produce films and digital content across subject areas. Their productions are often used as a catalyst for discussion in the classroom or to illustrate mastery of curriculum content. Producing films and digital content that are engaging and also successfully conveying educational messages is challenging. Filmmakers engage audiences by using film techniques to activate different emotions. Producing learning content that is engaging and activates those emotions that support learning requires an understanding of the craft of the filmmaker and those academic emotions that learners experience when learning. We present an evaluation tool based on those emotions that learners experience when learning (Pekrun, Goetz, Titz, & Perry, 2002) or those emotions different film techniques can provoke in viewers. The tool or Wheel of Academic Emotions (WAE) is useful for design thinking and for evaluating whether films and other digital productions activate the emotions known to support learning. We argue the WAE is timely because it provides teachers and students a useful tool to be creative and discerning decision-makers and to evaluate their digital content critically to ensure it communicates their ideas.
Relative Extraction Methodology for class diagram generation using dependency graph

Requirement analysis is the preliminary step in software development process. The requirements stated by the clients are analyzed and an abstraction of it is created which is termed as requirements model. Unified Modeling Language (UML) models are helpful for understanding the problems, communicating with application experts and preparing documentation. The static design view of the system can be modeled using a UML class diagram. System requirements stated by the user are usually in natural language form despite a wide variety of formal languages and UML. This is an imprecise and inconsistent form which is difficult to be used by the developer for design. We present a new methodology for generating UML class diagrams or models from natural language problem statement or requirement specification. We have named our methodology as Relative Extraction Methodology which uses an intermediate graphical representation called dependency graph. This serves as a skeleton for the generation of UML class models. The natural language processing is done for the construction of dependency graph which is finally mapped to a class diagram.
Calibration Method Evaluation via Depth Reconstruction

This paper presents a fast method to evaluate the accuracy of a calibration method of a stereovision rig built with commercially available cameras. The method allows by retro projection to quantify very precisely errors in terms of depth reconstruction. The proposed method has been evaluated on the depth reconstruction of real scenes from images taken with an unmanned aerial vehicle.