Miracle-dz/UniFAR-FTUs huggingface dataset:
Breast Cancer Bone Metastasis Identifies BACH 1 as a Master Regulator of Transcriptional Network Analysis

10.1074/jbc.M112.392332 Access the most updated version of this article at doi: . JBC Affinity Sites Find articles, minireviews, Reflections and Classics on similar topics on the Alerts: When a correction for this article is posted • When this article is cited • to choose from all of JBC's e-mail alerts Click here Supplemental material: http://www.jbc. org/content/suppl/2012/08/08/M112.392332. DC1.html
GPR30 is overexpressed in post-puberal testicular germ cell tumors

GPR30 is a 7-transmembrane G protein-coupled estrogen receptor that functions alongside traditional estrogen receptors to regulate cellular responses to 17β-estradiol and environmental estrogens. In this study, we have evaluated by immunohistochemical analysis GPR30 expression in post-puberal testicular germ cell tumours (30 seminomas, 5 teratomas, 12 embryonal carcinomas, and 20 intratubular germ cell tumors). The GPR30 protein expression was detected at high level in all intratubular germ cell tumours, seminomas, and embryonal carcinomas, whereas in teratomas the expression was low. The immunohistochemical data were further confirmed by Western blot analysis. GPR30 protein expression has also been analyzed in GC1 and TCam-2 cell lines, respectively derived from immortalized type B murine spermatogonia and human seminoma. Our results indicate that GPR30 could be a potential therapeutic target; the design of a specific GPR30 inhibitors could be a useful molecular target to block neoplastic germ cells with a high proliferative rate for the treatment of TGCTs. See commentary: Role of GPR30 in testicular germ cell tumors: A potential new anticancer target
Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis

A Bacillus subtilis 2.7-kilobase DNA fragment containing an intracellular protease gene was cloned into Escherichia coli. The transformants produced an intracellular protease of approximately 35,000 Mr whose activity was inhibited by both phenylmethylsulfonyl fluoride and EDTA. Introduction of the fragment on a multicopy vector, pUB110, into B. subtilis caused a marked increase in the level of the intracellular protease. The nucleotide sequence of the cloned fragment showed the presence of an open reading frame for a possible proenzyme of the major intracellular serine protease (ISP-I) of B. subtilis with an NH2-terminal 17- or 20-amino-acid extension. The total amino acid sequence of the protease deduced from the nucleotide sequence showed considerable homology with that of an extracellular serine protease, subtilisin. The transcriptional initiation site of the ISP-I gene was identified by nuclease S1 mapping. No typical conserved sequence for promoters was found upstream of the open reading frame. An ISP-I-negative mutant of B. subtilis was constructed by integration of artificially deleted gene into the chromosome. The mutant sporulated normally in a nutritionally rich medium but showed decreased sporulation in a synthetic medium. The chloramphenicol resistance determinant of a plasmid integrated at the ISP-I locus was mapped by PBS1 transduction and was found to be closely linked to metC (99.5%).
Thermoreflectance imaging of sub 100 ns pulsed cooling in high-speed thermoelectric microcoolers

Miniaturized thin film thermoelectric coolers have received considerable attention as potential means to locally address hot spots in microprocessors. Given the highly dynamic workload in complex integrated circuits, the need arises for a thorough understanding of the high-speed thermal behavior of microcoolers. Although some prior work on transient Peltier cooling in pulsed operation is available, these studies mostly focus on theoretical modeling and typically deal with relatively large modules with time constants well into the millisecond range. In this paper, we present an extensive experimental characterization of 30×30 μm2 high-speed coplanar SiGe superlattice microcoolers subjected to 300 ns wide current pulses at ≈ 300 kHz repetition rate. Using thermoreflectance imaging microscopy, we obtain 2D maps of the transient surface temperature and constituent Peltier and Joule components over the 50–750 ns time range with submicron spatial and 50 ns temporal resolutions. Net cooling of 1 K–1.5 K is achie.
It's the psychology stupid: how heuristics explain software vulnerabilities and how priming can illuminate developer's blind spots

Despite the security community's emphasis on the importance of building secure software, the number of new vulnerabilities found in our systems is increasing. In addition, vulnerabilities that have been studied for years are still commonly reported in vulnerability databases. This paper investigates a new hypothesis that software vulnerabilities are blind spots in developer's heuristic-based decision-making processes. Heuristics are simple computational models to solve problems without considering all the information available. They are an adaptive response to our short working memory because they require less cognitive effort. Our hypothesis is that as software vulnerabilities represent corner cases that exercise unusual information flows, they tend to be left out from the repertoire of heuristics used by developers during their programming tasks. To validate this hypothesis we conducted a study with 47 developers using psychological manipulation. In this study each developer worked for approximately one hour on six vulnerable programming scenarios. The sessions progressed from providing no information about the possibility of vulnerabilities, to priming developers about unexpected results, and explicitly mentioning the existence of vulnerabilities in the code. The results show that (i) security is not a priority in software development environments, (ii) security is not part of developer's mindset while coding, (iii) developers assume common cases for their code, (iv) security thinking requires cognitive effort, (v) security education helps, but developers can have difficulties correlating a particular learned vulnerability or security information with their current working task, and (vi) priming or explicitly cueing about vulnerabilities on-the-spot is a powerful mechanism to make developers aware about potential vulnerabilities.
Migration Towards Cloud-Assisted Live Media Streaming

Live media streaming has become one of the most popular applications over the Internet. We have witnessed the successful deployment of commercial systems with content delivery network (CDN)- or peer-to-peer-based engines. While each being effective in certain aspects, having an all-round scalable, reliable, responsive, and cost-effective solution remains an illusive goal. Moreover, today's live streaming services have become highly globalized, with subscribers from all over the world. Such a globalization makes user behaviors and demands even more diverse and dynamic, further challenging state-of-the-art system designs. The emergence of cloud computing, however, sheds new light into this dilemma. Leveraging the elastic resource provisioning from the cloud, we present Cloud-Assisted Live Media Streaming (CALMS), a generic framework that facilitates a migration to the cloud. CALMS adaptively leases and adjusts cloud server resources in a fine granularity to accommodate temporal and spatial dynamics of demands from live streaming users. We present optimal solutions to deal with cloud servers with diverse capacities and lease prices, as well as the potential latencies in initiating and terminating leases in real-world cloud platforms. Our solution well accommodates location heterogeneity, mitigating the impact from user globalization. It also enables seamless migration for existing streaming systems, e.g., peer-to-peer, and fully explores their potentials. Simulations with data traces from both cloud service providers (Amazon EC2 and SpotCloud) and a live streaming service provider (PPTV) demonstrate that CALMS effectively mitigates the overall system deployment costs and yet provides users with satisfactory streaming latency and rate.
Optical properties of porcine skin dermis between 900 nm and 1500 nm

The weak absorption of shortwave infrared light by skin tissues between 700 and 1500 nm offers an important window for diagnosis by optical means. The strong scattering of shortwave infrared light by the skin, however, presents a challenge to the modelling of light propagation through the skin and the understanding of skin optics. We have measured the collimated and diffuse transmittance and diffuse reflectance of porcine skin dermis samples within 30 h post-mortem. Monte Carlo simulations have been performed to inversely determine the absorption coefficient, scattering coefficient and anisotropy factor of the dermis samples in the spectral range from 900 to 1500 nm. We further analyse the sensitivity of the values of the parameters to the experimental errors and inverse calculation procedures. The state of the cellular integrity of the skin samples following optical measurements was verified using transmission electron microscopy. These results were correlated to study post-mortem effects on the in vitro optical properties of porcine dermis. We concluded that for samples stored within crushed ice for up to 30 h post-mortem the wavelength dependence of optical properties of the dermis remains unchanged while the values of the parameters vary moderately due to modification of the water content of the tissue.
Robust reserve provision by commercial buildings considering energy-constrained frequency signals

It has been shown that the heating, ventilation, and air conditioning (HVAC) systems of commercial buildings can offer ancillary services to power systems due to their large thermal inertia. In this paper, we propose a new control framework for scheduling and provision of frequency reserves by commercial building aggregations. The framework builds on a hierarchical control scheme: first, the reserve capacities are allocated among the buildings, e.g., on a daily basis, using techniques from robust optimization; second, a robust model predictive controller optimizes the HVAC system consumption typically every 30 minutes; and third, a feedback controller adjusts the consumption to provide reserves in real time. The framework incorporates methods for energy-constrained frequency signals, which are currently adopted by several transmission system operators for loads or storages. The proposed framework can be used to estimate the amount of reserves that can be extracted from commercial buildings, and we demonstrate its performance in simulations considering different building types and reserve characteristics. Our results indicate that, depending on the season, aggregations of 35−150 commercial buildings with an average floor area of 15000 m2 are enough to participate in reserve markets with a minimum bid size of 5 MW and products for energy-constrained frequency signals.
Spatiotemporal patterns of the Huntingtin‐interacting protein 1‐related gene in the mouse head

Huntingtin‐interacting protein 1‐related (Hip1r) was originally identified due to its homology to Huntingtin‐interacting protein 1, which contributes to the development of Huntington's disease (HD). We studied the expression of the mouse Hip1r (mHip1r) gene in the mouse head by in situ hybridization. In early embryogenesis at embryonic day (E) 13, mHip1r expression was especially prominent in the olfactory epithelium, cerebral cortex layer 1, cortical plate, and dentate gyrus. During later development from E15 to E17, strong expression of mHip1r transcripts continued to be observed in the olfactory epithelium, cortical plate, and dentate gyrus. Furthermore, not only the subplate and subventricular zone of the cortex, but also secretory glands, such as the nasal gland and the submandibular gland, were mHip1r‐positive. Other positive tissues included the retinal ganglion cells, vomeronasal organ, trigeminal ganglion, and the developing molar tooth. In the adult mouse brain, similar expression patterns were observed in the cerebral cortex layers and other brain regions except the cerebellum. Additionally, by using an antibody against mHip1r, we confirmed these expression patterns at the protein level. Specific expression of mHip1r in the embryonic brain and secretory glands suggests a possible role for Hip1r in normal development and in the pathology of HD.
Criatividade no trabalho docente segundo professores de história: limites e possibilidades

This study investigated pedagogical work organization’s components and personal/individual’s elements which act as facilitating or inhibiting factors to the History teacher’s creativity expression. The tarticipants were 16 5th to 8th grade History teachers from 5 public and 5 private schools in Brasilia, Federal District. Data were obtained through semi-structured interviews. A content analysis was used to analyze the data. Several factors that promote and limit creativity in teaching were identified. Freedom and passionate love for the work were the most outstanding facilitators. Dificulties in the teacher-student relationship were pointed as the most frequent constraint. This study contributes to uncovering some aspects which hinder teacher´s creative expression and others which characterize a favorable environment to the creativity expression of teachers.
Development of a reconstructed human skin model for angiogenesis

We have previously shown that reconstructed human skin engineered from autologous keratinocytes, fibroblasts, and sterilized donor allodermis stimulates angiogenesis within 5–7 days when placed on well‐vascularized wound beds in nude mice. When this reconstructed skin was used clinically in more demanding wound beds, some grafts were lost, possibly due to delayed vascularization. As this reconstructed skin lacks any endothelial cells, our aim in this study was to develop an angiogenic reconstructed skin model in which to explore strategies to improve angiogenesis both in vitro and in vivo. We report that culture of small‐vessel human dermal microvascular endothelial cells (HuDMECs) was achieved using magnetic beads coated with an antibody to platelet cell adhesion molecule as a means of purifying the culture. Keratinocytes, fibroblasts, and HuDMECs could be cultured from the same skin biopsy. Initial studies culturing HuDMECs and other sources of endothelial cells with the tissue‐engineered skin showed that these cells were capable of slowly entering the dermis under standard culture conditions in vitro. In conclusion, this provides us with a model in which to explore strategies for improving angiogenesis in vitro and also establishes the culture methodologies for the production of reconstructed skin containing autologous keratinocytes, fibroblasts, and endothelial cells. (WOUND REP REG 2003;11:275–284)
Semi-Blind Maximum Likelihood Joint Channel Estimation / Data Detection for MIMO Fading Channels

The aim of this paper is to investigate receiver techniques for ML joint channel/data estimation in flat fading MIMO channels that are both i) data efficient and ii) computationally attractive. The performance of iterative least squares (LS) for channel estimation combined with sphere decoding (SD) for data detection is examined in the first part, for block fading channels, demonstrating the data efficiency provided by the semi-blind approach. In the second part, the case of continuous fading channels is addressed with the aid of recursive least squares (RLS). The observed relative robustness of the ML solution to channel variations is exploited in deriving a block QR-based RLS-SD scheme, which allows significant complexity savings with little or no performance loss
Opposites Attract, but Similarity Works: A Study of Interorganizational Similarity in Marketing Channels

ABSTRACT This study explores the construct of interorganizational similarity in business orientation between manufacturers and distributors. Drawing on Social Identity Theory, the authors develop hypotheses concerning the outcomes of similarity on the relationship. In particular, they argue that relationship effectiveness for the manufacturer is positively affected by similarity in business orientation through the mediating construct of cooperation. An empirical study of more than 200 business relationships provides support for the theoretical reasoning.
Real-time panoramic tracking for event cameras

Event cameras are a paradigm shift in camera technology. Instead of full frames, the sensor captures a sparse set of events caused by intensity changes. Since only the changes are transferred, those cameras are able to capture quick movements of objects in the scene or of the camera itself. In this work we propose a novel method to perform camera tracking of event cameras in a panoramic setting with three degrees of freedom. We propose a direct camera tracking formulation, similar to state-of-the-art in visual odometry. We show that the minimal information needed for simultaneous tracking and mapping is the spatial position of events, without using the appearance of the imaged scene point. We verify the robustness to fast camera movements and dynamic objects in the scene on a recently proposed dataset [18] and self-recorded sequences.
On the use of NAND flash memory in high-performance relational databases

High-density NAND flash storage has become relatively inexpensive due to the popularity of various consumer electronics. Recently, several manufacturers have released IDE-compatible NAND flash-based drives in sizes up to 64 GB at reasonable (sub$1000) prices. Because flash is significantly more durable than mechanical hard drives and requires considerably less energy, there is some speculation that large data centers will adopt these devices. As database workloads make up a substantial fraction of the processing done by data centers, it is interesting to ask how switching to flash-based storage will affect the performance of database systems. We evaluate this question using IDE-based flash drives from two major manufacturers. We measure their read and write performance and find that flash has excellent random read performance, acceptable sequential read performance, and quite poor write performance compared to conventional IDE disks. We then consider how standard database algorithms are affected by these performance characteristics and find that the fast random read capability dramatically improves the performance of secondary indexes and index-based join algorithms. We next investigate using logstructured filesystems to mitigate the poor write performance of flash and find an 8.2x improvement in random write performance, but at the cost of a 3.7x decrease in random read performance. Finally, we study techniques for exploiting the inherent parallelism of multiple-chip flash devices, and we find that adaptive coding strategies can yield a 2x performance improvement over static ones. We conclude that in many cases flash disk performance is still worse than on traditional drives and that current flash technology may not yet be mature enough for widespread database adoption if performance is a dominant factor. Finally, we briefly speculate how this landscape may change based on expected performance of next-generation flash memories. Thesis Supervisor: Samuel R. Madden Title: ITT Career Development Professor
Automatic categorization and summarization of documentaries

In this paper, we propose automatic categorization and summarization of documentaries using subtitles of videos. We propose two methods for video categorization. The first makes unsupervised categorization by applying natural language processing techniques on video subtitles and uses the WordNet lexical database and WordNet domains. The second has the same extraction steps but uses a learning module to categorize. Experiments with documentary videos give promising results in discovering the correct categories of videos. We also propose a video summarization method using the subtitles of videos and text summarization techniques. Significant sentences in the subtitles of a video are identified using these techniques and a video summary is then composed by finding the video parts corresponding to these summary sentences.
The Persistence of Workplace Gender Segregation in the US

Occupational gender segregation remains one of the defining elements of gender inequality in modern societies. Recent trends for the United States show that occupational segregation remains high and did not substantially decline in the decade of the 2000s for the first time since 1960. Men and women work in different occupations because of a combination of forces, including culturally defined choices by workers themselves, discrimination by employers, and differences in skill levels and qualities. Research has shown that occupational segregation is an important aspect of gender inequality in earnings and contributes to other forms of inequality as well. The prospects for reducing gender segregation in the short term appear slim, based on the weak effects of educational attainment, cultural attitudes, and state intervention in the current period.
Breast-Feeding Is Associated With a Reduced Frequency of Acute Otitis Media and High Serum Antibody Levels Against NTHi and Outer Membrane Protein Vaccine Antigen Candidate P6

Nontypeable Haemophilus influenzae (NTHi) causes acute otitis media (AOM) in infants. Breast-feeding protects against AOM and/or nasopharyngeal (NP) colonization; however, the mechanism of protection is incompletely understood. Children with AOM and healthy children were studied according to feeding status: breast-fed, breast/formula fed, or formula fed. Cumulative episodes of AOM, ELISA titers of serum IgG antibodies to whole-cell NTHi and vaccine candidate outer membrane protein P6, bactericidal titers of serum and NP colonization by NTHi were assessed. A lower incidence of AOM was found in breast- versus formula-fed children. Levels of specific serum IgG antibody to NTHi and P6 were highest in breast-fed, intermediate in breast/formula fed, and lowest in formula-fed infants. Serum IgG antibody to P6 correlated with bactericidal activity against NTHi. Among children with AOM, the prevalence of NTHi in the NP was lower in breast- versus nonbreast-fed infants. We conclude that breast-feeding shows an association with higher levels of antibodies to NTHi and P6, suggesting that breast-feeding modulates the serum immune response to NTHi and P6. Higher serum IgG might facilitate protection against AOM and NP colonization in breast-fed children.
Basic methods for fission yeast

The fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in budding yeast. Here, we present basic methods sufficient for a beginner in this system to carry out most required manipulations for genetic analysis or molecular biology. Copyright © 2006 John Wiley & Sons, Ltd.
Anterior chamber depth in angle-closure glaucoma. Clinical methods of depth determination in people with and without the disease.

The sensitivity and specificity of the Van Herick slitlamp test and the oblique illumination test for estimating the clinical depth of the anterior chamber were found to be sensitive methods of determining shallow anterior chambers. The same tests showed a high sensitivity in separating patients with and without known attacks of angle-closure glaucoma. It is suggested that the use of either of these tests seems a reliable way of spotting the shallow anterior chambers that can lead to subsequent angle-closure glaucoma attacks.