Miracle-dz/UniFAR-FTUs huggingface dataset:
A discipline-mediated model of excessively punitive parenting

This study developed and compared several mediated and direct-effects models proposed to link well-established correlates of physical child abuse to excessively punitive parenting in a population-based sample of 206 families recruited from high-risk areas of a medium-sized metropolitan area, Parents mere classified as exhibiting excessively punitive parenting based on home observations and parent reports of the use of physical punishment strategies other than spanking, We hypothesized that the levels of child coerciveness, parent irritability, and family stress would be mediated by disruptions in parent discipline in predicting excessively punitive parenting toward the child. Structural equation methodology was employed to test the hypothesized and alternative models. Results consistently supported the powerful mediational role of discipline in the hypothesized model over alternative models that posited separate, direct effects of child coerciveness, parent irritability, and parent stress on excessively punitive parenting behavior. Results are discussed in terms of the need for further examination of parenting behavior, as well as parents' perceptions of and affective reactions to parent-child interactions in the occurrence of physical abuse.
Inhibition of mTOR sensitizes breast cancer stem cells to radiation-induced repression of self-renewal through the regulation of MnSOD and Akt

The sensitization of breast cancer stem cells (BrCSCs) to the inhibitive effects of radiotherapy through adjuvant therapy which targets oncogenic pathways represents a prospective strategy for improving the effect of radiation in patients with triple-negative breast cancer (TNBC). Mammalian target of rapamycin (mTOR) activation is one of the most frequent events in human malignancies, and is critical for sustaining the self-renewing ability of cancer stem cells (CSCs); inhibition by rapamycin is an effective and promising strategy in anticancer treatments. In the present study, we found that mTOR activity was closely related to the self-renewal ability of BrCSCs, and in triple negative MDA-MB-453 and MDA-MB-468 cells, rapamycin repression of mTOR phosphorylation decreased the number of mammospheres and helped to sensitize the resistant CSCs to low-dose radiation therapy. By inhibiting mTOR and mitochondrial manganese superoxide dismutase (MnSOD), we confirmed that rapamycin functioned through the mTOR/MnSOD/reactive oxygen species (ROS) signaling pathway, and the existence of Akt governed the rapamycin-induced asymmetric division (AD) of stem cells in cases of radiation-treated breast cancer. The synergic effects of rapamycin and low-dose radiation induced the AD of stem cells, which then resulted in a decrease in the number of mammospheres, and both were mediated by MnSOD. Governed by Akt, the consequent inhibition of ROS formation and oxidative stress preserved the AD mode of stem cells, which is critical for an improved radiotherapy response in clinical treatment, as the tumor group is thus easier to eliminate with radiation therapy. We posit that an in-depth understanding of the interaction of radiation with CSCs has enormous potential and will make radiation even better and more effective.
Siberian Sturgeon Oocyte Extract Induces Epigenetic Modifications of Porcine Somatic Cells and Improves Developmental Competence of SCNT Embryos

Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is induced by exposure of introduced donor nuclei to the recipient cytoplasm of matured oocytes. However, because the efficiency of SCNT still remains low, a combination of SCNT technique with the ex-ovo method may improve the normal development of SCNT embryos. Here we hypothesized that treatment of somatic cells with extracts prepared from the germinal vesicle (GV) stage Siberian sturgeon oocytes prior to their use as nuclear donor for SCNT would improve in vitro development. A reversible permeability protocol with 4 μg/mL of digitonin for 2 min at 4°C in order to deliver Siberian sturgeon oocyte extract (SOE) to porcine fetal fibroblasts (PFFs) was carried out. As results, the intensity of H3K9ac staining in PFFs following treatment of SOE for 7 h at 18°C was significantly increased but the intensity of H3K9me3 staining in PFFs was significantly decreased as compared with the control (p<0.05). Additionally, the level of histone acetylation in SCNT embryos at the zygote stage was significantly increased when reconstructed using SOE-treated cells (p<0.05), similar to that of IVF embryos at the zygote stage. The number of apoptotic cells was significantly decreased and pluripotency markers (Nanog, Oct4 and Sox2) were highly expressed in the blastocyst stage of SCNT embryos reconstructed using SOE-treated cells as nuclear donor (p<0.05). And there was observed a better development to the blastocyst stage in the SOE-treated group (p<0.05). Our results suggested that pre-treatment of cells with SOE could improve epigenetic reprogramming and the quality of porcine SCNT embryos.
Muscle glutathione metabolism during ischemia and reperfusion in patients undergoing aorto‐bifemoral bypass surgery

Background: After an ischemia time of 1 h during aortic aneurysm surgery, muscle glutathione redox‐status is not altered, indicating that this ischemic insult is well within the scavenging capacity of muscle glutathione, the most important endogenous scavenger. In the present study, the impact of elective aorto‐bifemoral bypass surgery, involving a longer ischemia time, on muscle glutathione and its redox‐status was investigated.
The role of founder effects on the evolution of reproductive isolation

Several theories argue that large changes in allele frequencies through genetic drift after a small founding population becomes allopatrically isolated can lead to significant changes in reproductive isolation and thus trigger the origin of new species. For this reason, founder speciation has been proposed as a potent force in the generation of new species. Nonetheless, the relative importance of such ‘founder effects’ remains largely untested. In this report, I used experimental evolution to create one thousand replicates that underwent an extreme bottleneck and to study whether founder effects can lead to an increase in reproductive isolation in Drosophila yakuba. Even though the most common outcome of inbreeding is extinction, founder effects can lead to increased premating reproductive isolation in a very small proportion of cases. Changes in reproductive isolation after a founding population bottleneck are similar to changes in other phenotypic traits, in which inbreeding might displace the mean phenotypic value and substantially increase the phenotypic variance. This increase in phenotypic variance does not confer an increase in the response to selection for reproductive isolation in artificial selection experiments, indicating that the increased phenotypic variance is not caused by increases in additive genetic variance. These results also demonstrate that, similar to morphological and life‐history traits, behavioural traits can be affected by inbreeding and genetic drift.
Homeless students and resilience: Staff perspectives on individual and environmental factors

The resilience paradigm identifies key contributors to children's competence despite adversity, and offers a theoretical framework for supporting the healthy development of children and youth who are homeless. This study used a resilience framework to gather and analyze the perspectives of staff members about homeless children's characteristics, needs, and recommended interventions across different types of homeless education programs.
An FtsZ-Targeting Prodrug with Oral Antistaphylococcal Efficacy In Vivo

ABSTRACT The bacterial cell division protein FtsZ represents a novel antibiotic target that has yet to be exploited clinically. The benzamide PC190723 was among the first FtsZ-targeting compounds to exhibit in vivo efficacy in a murine infection model system. Despite its initial promise, the poor formulation properties of the compound have limited its potential for clinical development. We describe here the development of an N-Mannich base derivative of PC190723 with enhanced drug-like properties and oral in vivo efficacy. The N-Mannich base derivative (TXY436) is ∼100-fold more soluble than PC190723 in an acidic aqueous vehicle (10 mM citrate, pH 2.6) suitable for oral in vivo administration. At physiological pH (7.4), TXY436 acts as a prodrug, converting to PC190723 with a conversion half-life of 18.2 ± 1.6 min. Pharmacokinetic analysis following intravenous administration of TXY436 into mice yielded elimination half-lives of 0.26 and 0.96 h for the TXY436 prodrug and its PC190723 product, respectively. In addition, TXY436 was found to be orally bioavailable and associated with significant extravascular distribution. Using a mouse model of systemic infection with methicillin-sensitive Staphylococcus aureus or methicillin-resistant S. aureus, we show that TXY436 is efficacious in vivo upon oral administration. In contrast, the oral administration of PC190723 was not efficacious. Mammalian cytotoxicity studies of TXY436 using Vero cells revealed an absence of toxicity up to compound concentrations at least 64 times greater than those associated with antistaphylococcal activity. These collective properties make TXY436 a worthy candidate for further investigation as a clinically useful agent for the treatment of staphylococcal infections.
Bidirectional switch commutation for high frequency UPF matrix converter supplying Inductive Power Transfer system

There are abundant application for contactless power transfer in mechanical engineering, production technology, transportation and medical engineering; conventional systems are associated with the losses caused by high frequency multiple energy conversion and primary inverter. Therefore novel power electronic topologies for direct energy conversion are crucial for this application. The use of matrix converters for contactless energy transmission reduces the number of energy conversion steps, avoid voluminous and expensive electrolytic DC link capacitors and enable four quadrant operations. This paper proposes application of three phase to single phase matrix converter with sinusoidal input line current supplying a series resonant Inductive Power Transfer (IPT) device. Minimization of commutation steps regardless of the load current direction is one of the main merits of proposed topology.
Addressing disparities: The impact of a lesbian, gay, bisexual, and transgender graduate counselling course

AbstractBackground: Lesbian, gay, bisexual and transgender (LGBT) individuals experience serious mental health disparities and treatment inequities. Counsellor education has been identified as both a contributing factor to these problems as well as an ameliorating mechanism to address these inequalities. Aim: The purpose of the current study was to evaluate the impact of an LGBT-affirmative counselling course. Method: A total of 23 students enrolled in a graduate LGBT counselling course were administered the Sexual Orientation Counselor Competency Scale (SOCCS, Bidell, 2005) and the Lesbian, Gay, and Bisexual Affirmative Counseling Self-Efficacy Inventory (LGB-CSI, Dillon & Worthington, 2003) pre- and post-course. In addition, a comparison group of 23 matched counselling students was obtained from an existing data source in order to make further assessments of the course's effectiveness. Those in the comparison group were not enrolled in the LGBT course, but were administered the SOCCS at analogous time i.
A Unified Approach to Dynamic Matching and Barter Exchange

Abstract : The exchange of indivisible goods without money addresses a variety of constrained economic settings where a medium of exchange such as money is considered inappropriate. Participants are either matched directly with another participant or, in more complex domains, in barter cycles and chains with many other participants before exchanging their endowed goods. This thesis addresses the design, analysis, and real-world fielding of dynamic matching markets and barter exchanges. We present new mathematical models for static and dynamic barter exchange that more accurately reflect reality, prove theoretical statements about the characteristics and behavior of these markets, and develop provably optimal market clearing algorithms for models of these markets that can be deployed in practice. We show that taking a holistic approach to balancing efficiency and fairness can often practically circumvent negative theoretical results. We support the theoretical claims made in this thesis with extensive experiments on data from the United Network for Organ Sharing(UNOS) Kidney Paired Donation Pilot Program, a large kidney exchange clearinghouse in the US with which we have been actively involved. Specifically, we study three competing dimensions found in both matching markets and barter exchange: uncertainty over the existence of possible trades (represented as edges in a graph constructed from participants in the market), balancing efficiency and fairness, and inherent dynamism. For each individual dimension, we provide new theoretical insights as to the effect on market efficiency and match composition of clearing markets under models that explicitly consider those dimensions. We support each theoretical construct with new optimization models and techniques, and validate them on simulated and real kidney exchange data.
An Extensible Toolkit for Computational Semantics

In this paper we focus on the software for computational semantics provided by the Python-based Natural Language Toolkit (nltk). The semantics modules in nltk are inspired in large part by the approach developed in Blackburn and Bos (2005) (henceforth referred to as B&B). Since Blackburn and Bos have also provided a software suite to accompany their excellent textbook, one might ask what the justification is for the nltk offering, which is similarly slanted towards teaching computational semantics.
Netrin-1 Promotes Thalamic Axon Growth and Is Required for Proper Development of the Thalamocortical Projection

The thalamocortical axon (TCA) projection originates in dorsal thalamus, conveys sensory input to the neocortex, and has a critical role in cortical development. We show that the secreted axon guidance molecule netrin-1 acts in vitro as an attractant and growth promoter for dorsal thalamic axons and is required for the proper development of the TCA projection in vivo. As TCAs approach the hypothalamus, they turn laterally into the ventral telencephalon and extend toward the cortex through a population ofnetrin-1-expressing cells. DCC andneogenin, receptors implicated in mediating the attractant effects of netrin-1, are expressed in dorsal thalamus, whereas unc5h2 and unc5h3, netrin-1 receptors implicated in repulsion, are not. In vitro, dorsal thalamic axons show biased growth toward a source of netrin-1, which can be abolished by netrin-1-blocking antibodies. Netrin-1 also enhances overall axon outgrowth from explants of dorsal thalamus. The biased growth of dorsal thalamic axons toward the internal capsule zone of ventral telencephalic explants is attenuated, but not significantly, by netrin-1-blocking antibodies, suggesting that it releases another attractant activity for TCAs in addition to netrin-1. Analyses ofnetrin-1 −/− mice reveal that the TCA projection through the ventral telencephalon is disorganized, their pathway is abnormally restricted, and fewer dorsal thalamic axons reach cortex. These findings demonstrate that netrin-1 promotes the growth of TCAs through the ventral telencephalon and cooperates with other guidance cues to control their pathfinding from dorsal thalamus to cortex.
Predicting anchor links between heterogeneous social networks

People usually get involved in multiple social networks to enjoy new services or to fulfill their needs. Many new social networks try to attract users of other existing networks to increase the number of their users. Once a user (called source user) of a social network (called source network) joins a new social network (called target network), a new inter-network link (called anchor link) is formed between the source and target networks. In this paper, we concentrated on predicting the formation of such anchor links between heterogeneous social networks. Unlike conventional link prediction problems in which the formation of a link between two existing users within a single network is predicted, in anchor link prediction, the target user is missing and will be added to the target network once the anchor link is created. To solve this problem, we propose an effective general meta-path-based approach called Connector and Recursive Meta-Paths (CRMP). By using those two different categories of meta-paths, we model different aspects of social factors that may affect a source user to join the target network, resulting in the formation of a new anchor link. Extensive experiments on real-world heterogeneous social networks demonstrate the effectiveness of the proposed method against the recent methods.
Empowerment within the UK construction sector

The feasibility and success of empowerment are to some extent determined by factors relating to the culture and structure of the industries within which organizations are embedded. The UK construction industry is an example that has a unique socio‐technical context, some aspects of which seem well‐suited to empowerment strategies. However, despite support for empowerment, it has not yet been universally embraced by construction organizations. Outlines the aspects of the industry that provide a suitable context for implementation of empowerment strategies. Suggests that, used selectively, it could play an important part in helping construction organizations to address increasing performance demands whilst mitigating the negative effects of the fragmented project delivery process. However, there remain many barriers to individual and team‐based empowerment strategies that require empirical investigation before the industry can benefit from their implementation.
PLAYER - a European Project and a Game to Foster Entrepreneurship Education for Young People

Entrepreneurship is widely recognized as one of the basic skills to be acquired through a life-long learning. The European Union, under the guidance of the Oslo Agenda, promotes several initiatives to develop entrepreneurship culture in Europe. Education can make a significant contribution to entrepreneurship, encouraging the development of entrepreneurial attitudes and skills in young people. Serious Games are presently recognised as having an important role and potential in education and social networks emerged in the last years as the platform preferred by many, especially young people, to socialize, play games and even learn. This paper presents the PLAYER project, in which a game was developed and implemented as a Facebook application, to enable learning entrepreneurial skills progressively, by guiding users to develop a business idea in the form of a business plan.
Applying Objective Diagnostic Criteria to Students in a College Support Program for Learning Disabilities

This study examined whether a large group of postsecondary students participating in a support program for students classified as having learning disabilities (LD) met criteria for five objective diagnostic models for LD: IQ-achievement discrepancy (1.0 SD, 1.5 SD, and >2.0 SD) models, a Diagnostic and Statistical Manual of Mental Disorders (DSM-IV) model, and an academic impairment model. The participants were 336 postsecondary students enrolled in a private liberal arts college who had been previously classified as having LD prior to entering the program. Results showed that the largest proportion of students met the 1.0 SD (48.8%) and the absolute academic impairment (40.2%) criteria. However, 40.5% of the participants were not classified as having LD by any of the five models. Participants exhibited remarkable variability in their aptitude and achievement scores, with mean composite achievement scores ranging from the very low to the average ranges in reading, mathematics, and writing, depending on the achievement test used. The findings of this study and others suggest that little information about a student’s academic skills is likely to be conveyed by a diagnosis of LD, leading to great heterogeneity in skill levels within LD support programs.
Structural and Thermodynamic Basis of Epitope Binding by Neutralizing and Nonneutralizing Forms of the Anti-HIV-1 Antibody 4 E 10

The 4E10 antibody recognizes the membrane-proximal external region (MPER) of the HIV-1 Env glycoprotein gp41 transmembrane subunit, exhibiting one of the broadest neutralizing activities known to date. The neutralizing activity of 4E10 requires solvent-exposed hydrophobic residues at the apex of the complementarity-determining region (CDR) H3 loop, but the molecular basis for this requirement has not been clarified. Here, we report the cocrystal structures and the energetic parameters of binding of a peptide bearing the 4E10-epitope sequence (4E10ep) to nonneutralizing versions of the 4E10 Fab. Nonneutralizing Fabs were obtained by shortening and decreasing the hydrophobicity of the CDR-H3 loop (termed Loop) or by substituting the two tryptophan residues of the CDR-H3 apex with Asp residues (termed WDWD), which also decreases hydrophobicity but preserves the length of the loop. The analysis was complemented by the first crystal structure of the 4E10 Fab in its ligand-free state. Collectively, the data ruled out major conformational changes of CDR-H3 at any stage during the binding process (equilibrium or transition state). Although these mutations did not impact the affinity of wild-type Fab for the 4E10ep in solution, the two nonneutralizing versions of 4E10 were deficient in binding to MPER inserted in the plasma membrane (mimicking the environment faced by the antibody in vivo). The conclusions of our structure-function analysis strengthen the idea that to exert effective neutralization, the hydrophobic apex of the solvent-exposed CDR-H3 loop must recognize an antigenic structure more complex than just the linear -helical epitope and likely constrained by the viral membrane lipids.
Distinct roles for antiparallel microtubule pairing and overlap during early spindle assembly

Correlation of spindle architecture with dynamic behavior shows that pairs of antiparallel microtubules are sufficient to form a bipolar spindle, whereas interpolar microtubules maintain the speed of pole displacement during spindle assembly. The number of interpolar microtubules formed is controlled in part through γ-tubulin phosphorylation.
Robust face tracking using color

We discuss a new robust tracking technique applied to histograms of intensity-normalized color. This technique supports a video codec based on orthonormal basis coding. Orthonormal basis coding can be very efficient when the images to be coded have been normalized in size and position. However an imprecise tracking procedure can have a negative impact on the efficiency and the quality of reconstruction of this technique, since it may increase the size of the required basis space. The face tracking procedure described in this paper has certain advantages, such as greater stability, higher precision, and less jitter, over conventional tracking techniques using color histograms. In addition to those advantages, the features of the tracked object such as mean and variance are mathematically describable.