Miracle-dz/UniFAR-FTUs huggingface dataset:
Emergence of Aspergillus calidoustus Infection in the Era of Posttransplantation Azole Prophylaxis

Background Universal antifungal prophylaxis with azoles is commonly used after lung transplantation. We noted an increase in isolates of Aspergillus calidoustus in our transplant population and hypothesized that increasing azole use (universal prophylaxis since 2008) may be promoting this infection. Methods Clinical and microbiologic data for A. calidoustus cases from 2008 to 2011 were extracted from chart review. For lung transplant patients, a case-control study was performed to determine risk factors, and incidence rates were calculated. Results From 2008 to 2011, we identified seven organ transplant recipients and one hematopoietic stem-cell transplant patient with positive A. calidoustus culture results in bronchoalveolar lavage at a median of 13 months after transplantation (interquartile range, 4–39 months). Chest computed tomographic scan was consistent with fungal infection in six of eight patients, and the European Organization for Research and Treatment of Cancer/Mycoses Study Group criteria classified these as “probable” invasive aspergillosis. In the case-control study, there were no differences in immunosuppression, number of respiratory samples taken, length of intensive care unit stay, or rejection rates. Of controls, 33.3% received third-generation azole prophylaxis compared with 83.3% of cases (P=0.13). However, median duration of exposure was greater in cases than in controls (3 vs. 0 months, P=0.045). Fungal minimum inhibitory concentration for voriconazole was 4 µg/mL or greater for six of eight cases. Incidence rates in lung transplants showed an increase of A. calidoustus (0/1000 vs. 11.3/1000 patient-years in 2006–2007 and 2008–2011, respectively; P=0.018), whereas Aspergillus fumigatus cases decreased (73.9/1000 vs. 49.0/1000 patient-years, P=0.0066). Conclusions Pulmonary A. calidoustus seems to be an emerging pathogen mainly in lung transplants. We suggest that third-generation azole use reduced the incidence of A. fumigatus, but the incidence of A. calidoustus, an azole-resistant fungus, was increased.
ProSy: A similarity based inline deduplication system for primary storage

Data deduplication can reduce cost and enhance throughput in backup and archiving systems. Recently, it becomes increasingly popular to apply this technique in primary storage systems, where data is actively used by enterprise business applications. However, the state-of-the-art deduplication systems for primary storages mainly provide offline solutions, which require sufficient time-window, additional space and energy. The biggest challenge for an inline deduplication solution is the acceptable performance in terms of data deduplication ratio, access latency, system throughput and management overhead. In this paper, we propose a high accuracy similarity algorithm, and based on it, construct ProSy, a real-time inline deduplication system for primary storage, which can achieve acceptable comprehensive performance without requiring file layout information. Prosy is more reliable since it uses byte-by-byte comparison instead of strong hash comparison to guarantee data integrity. The main idea behind ProSy is to minimize the size of comparison set by grouping similar file segments into the same category when performing data deduplication. For each segment of files, ProSy searches for common data only within the category which this segment belongs to. The experimental evaluation based on real world datasets shows that ProSy is practical and it achieves satisfactory performance. Comparing with the common file system, ProSy can achieve more than 60% of the max data deduplication ratio, 27% deduction on latency, about 2.7% CPU utilization, 83% write throughput and 144% read throughput.
The effect of gap formation at the repair site on the strength and excursion of intrasynovial flexor tendons. An experimental study on the early stages of tendon-healing in dogs.

BACKGROUND Elongation (gap formation) at the repair site has been associated with the formation of adhesions and a poor functional outcome after repair of flexor tendons. Our objectives were to evaluate the prevalence of gap formation in a clinically relevant canine model and to assess the effect of gap size on the range of motion of the digits and the mechanical properties of the tendons. METHODS We performed operative repairs after sharp transection of sixty-four flexor tendons in thirty-two adult dogs. Rehabilitation with passive motion was performed daily until the dogs were killed at ten, twenty-one, or forty-two days postoperatively. Eight tendons ruptured in vivo. In the fifty-six intact specimens, the change in the angles of the proximal and distal interphalangeal joints and the linear excursion of the flexor tendon were measured as a 1.5-newton force was applied to the tendon. The gap at the repair site was then measured, and the isolated tendons were tested to failure in tension. RESULTS Twenty-nine tendons had a gap of less than one millimeter, twelve had a gap of one to three millimeters, and fifteen had a gap of more than three millimeters. Neither the time after the repair nor the size of the gap was found to have a significant effect on motion parameters (p > 0.05); however, the ultimate force, repair-site rigidity, and repair-site strain at twenty newtons were significantly affected by these parameters (p < 0.05). Testing of the tendons with a gap of three millimeters or less revealed that, compared with the ten-day specimens, the forty-two-day specimens failed at a significantly (90 percent) higher force (p < 0.01) and had a significantly (320 percent) increased rigidity (p < 0.01) and a significantly (60 percent) decreased strain at twenty newtons (p < 0.05). In contrast, the tensile properties of the tendons that had a gap of more than three millimeters did not change significantly with time. CONCLUSIONS Our data indicate that, in a dog model involving sharp transection followed by repair, a gap at the repair site of more than three millimeters does not increase the prevalence of adhesions or impair the range of motion but does prevent the accrual of strength and stiffness that normally occurs with time.
Prediction of Anti-cancer Nanotherapy Efficacy by Imaging

Anticancer nanotherapeutics have shown mixed results in clinical trials, raising the questions of whether imaging should be used to i) identify patients with a higher likelihood of nanoparticle accumulation, ii) assess nanotherapeutic efficacy before traditional measures show response, and iii) guide adjuvant treatments to enhance therapeutic nanoparticle (TNP) delivery. Here we review the use of a clinically approved MRI nanoparticle (ferumoxytol, FMX) to predict TNP delivery and efficacy. It is becoming increasingly apparent that nanoparticles used for imaging, despite clearly distinct physicochemical properties, often co-localize with TNP in tumors. This evidence offers the possibility of using FMX as a generic “companion diagnostic” nanoparticle for multiple TNP formulations, thus potentially allowing many of the complex regulatory and cost challenges of other approaches to be avoided.
“Wouldn’t You Walk Away?” Foreclosures and Homeowner Understandings

Social work scholarship on neoliberalism—the dominant ideology and policies shaping access to housing, jobs, healthcare, and education—is in its infancy. This study examines the ground-level impact of the subprime mortgage crisis that triggered the Great Recession in 2008, examining how homeowners interpreted the changes to their neighborhood as they witnessed a remarkably high rate of foreclosures during the economic collapse of 2008-2010. Residents of a suburban community were unaware of the lending and banking practices that transformed their neighborhoods, though these policies arguably depreciated house values and a sense of well-being. Not knowing the culpability of predatory lenders in the crisis, some residents turned to an anti-immigrant social movement to preserve their community.
Comparing Percutaneous Physeal Epiphysiodesis and Eight-Plate Epiphysiodesis for the Treatment of Limb Length Discrepancy

Background: Either percutaneous or eight-plate epiphysiodesis have been shown to be effective growth modulation techniques for the treatment of limb length discrepancies (LLD). However, few studies compared the outcomes of both techniques with some confounding results. The aim of this study was to evaluate the outcomes of the both techniques in the treatment of the LLD. Methods: Between 2004 and 2012, medical records of all cases that underwent either eight-plate or percutaneous epiphysiodesis (PE) were reviewed. Age at surgery, sex, diagnosis, surgical site (proximal tibia/distal femur), time of follow-up, complications, and additional procedures were noted. Correction of the LLD was evaluated to calculate the rate of correction and the percentage of improvement from the long-leg standing x-rays or scanograms, collected at each visit. Results: There were 24 patients in the eight-plate (10 girls and 14 boys) and 48 patients in the PE group (28 girls and 20 boys). No statistically significant difference was found in the age, sex, preoperative LLD, or follow-up between groups. Both groups reached to an average LLD below 2 cm. The percentage of improvement was significantly higher in the PE group (P=0.031). The rate of individual femoral and tibial correction did not differ between the groups. Conclusions: Both methods are shown to be effective for LLD correction. However, PE led to greater improvement during the same follow-up time with fewer complications and less need for additional surgical procedures.
Vra4 Congenic Rats with Allelic Differences in the Class II Transactivator Gene Display Altered Susceptibility to Experimental Autoimmune Encephalomyelitis1

Presentation of Ag bound to MHC class II (MHC II) molecules to CD4+ T cells is a key event in adaptive immune responses. Genetic differences in MHC II expression in the rat CNS were recently positioned to allelic variability in the CIITA gene (Mhc2ta), located within the Vra4 locus on rat chromosome 10. In this study, we have examined reciprocal Vra4-congenic strains on the DA and PVGav1 backgrounds, respectively. After experimental nerve injury the strain-specific MHC II expression on microglia was reversed in the congenic strains. Similar findings were obtained after intraparenchymal injection of IFN-γ in the brain. Expression of MHC class II was also lower on B cells and dendritic cells from the DA.PVGav1-Vra4- congenic strain compared with DA rats after in vitro stimulation with IFN-γ. We next explored whether Vra4 may affect the outcome of experimental autoimmune disease. In experimental autoimmune encephalomyelitis induced by immunization with myelin oligodendrocyte glycoprotein, DA.PVGav1-Vra4 rats displayed a lower disease incidence and milder disease course compared with DA, whereas both PVGav1 and PVGav1.DA-Vra4 rats were completely protected. These results demonstrate that naturally occurring allelic differences in Mhc2ta have profound effects on the quantity of MHC II expression in the CNS and on immune cells and that this genetic variability also modulates susceptibility to autoimmune neuroinflammation.
Social support and arterial pressure in a central Mexican community.

&NA; Research on the relationship of social support and arterial blood pressure was conducted in a town in central Mexico. A culturally appropriate measure of social support was developed assessing perceived support from relatives, friends, neighbors, and compadres (ritual kin). The effects of perceived support from these four sources were compared to the effects of the amount of reported social contacts. For males, more support from all four sources was related to lower arterial pressure, whereas amount of social contact was related to higher diastolic blood pressure; support from compadres had the largest inverse correlation with blood pressure. For females the pattern of correlations was dependent on age: support from relatives and friends was related to higher blood pressure for younger females, whereas support from relatives was related to lower blood pressure for older females. This pattern of relationships is consistent with the place of women in the social structure of this Mexican community, and these results point to the need to conceptualize and measure social support in culturally appropriate ways.
Presence of prostate cancer metastasis correlates with lower lymph node reactivity.

BACKGROUND Several reports suggest that the dissemination of neoplastic cells and cancer progression are associated with the generation of an immunosuppressive environment. METHODS In this report, we investigated immunological effects of prostate cancer by comparing metastastic and non-metastatic pelvic lymph nodes (LNs) from 25 patients with carcinomatous involvement of LNs to the non-metastatic LNs from 26 control patients with no metastatic involvement by immunohistochemistry and histological analyses. RESULTS Our results showed a decreased abundance of CD20+ B lymphocytes (P = 0.031), CD38+ activated lymphocytes (P = 0.038), and CD68+ macrophages (P < 0.001), and less evidence of follicular hyperplasia (P = 0.014), sinus hyperplasia (P < 0.001), and fibrosis (P=0.028) in metastatic LNs comparatively to control LNs. Finally, we observed that metastatic LNs were significantly smaller than control LNs (P = 0.005). CONCLUSIONS Our results suggest that the development of prostate cancer LN metastasis is accompanied with smaller LN size and decreased LN reactivity suggesting the development of an immununosuppressive microenvironment.
RHyThM, a tool for analysis of PDOS formatted hyperthermia treatment data generated by the BSD2000/3D system

One of the systems used by hyperthermia (HT) groups for heating tumours in the pelvic region is the BSD2000 system. Previous versions of the BSD2000 operate on a PDOS machine and the majority of the currently installed BSD2000/3D systems are still running under PDOS. Availability of the PDOS formatted treatment data provided by the BSD2000/3D has some difficulties. To facilitate analysis of the PDOS formatted treatment data generated by the BSD2000/3D a programme, called RHyThM (Rotterdam Hyperthermia Thermal Modulator) has been created. The purpose of RHyThM is first to read and check the integrity and validity of the treatment data for each measurement in time and space as provided by the BSD2000/3D and secondly to register a tissue type, based on computer tomography information, for each temperature probe position. Prior to any analyses, RHyThM shows the temperature profiles enabling the user to check on probe movement and to correct for unrealistically high temperature gradients in time and space. Subsequently, this approved data set is saved in a ‘mother-file’ for future on-demand thermal dose analyses. A unique feature of RHyThM is that it also shows all radiofrequency (RF) power signals for inspection. Finally, to make a quick assessment of the quality of the applied HT-treatment, RHyThM reports several temperature indices for bladder, vagina and rectum as well as RF-power related quantities. In summary, RHyThM is considered a valuable tool as it quickly provides a quality index per treatment, which serves as input for the preparation of the next treatment. Further, it makes verified and improved primary data sets accessible for further analysis with advanced statistical programmes.
Money laundering and audit fees

We investigate the association between state-level money laundering sentences and audit fees in the US. Money laundering measures a broad category of offenses involving financial transactions using funds or monetary instruments gained through criminal activities and tax evasion. We find that firms headquartered in US states with high rates of money laundering sentences pay more audit fees. Our results suggest that auditors incorporate, as a fee premium, the higher risks involved when clients operate in those states. Our result remains robust to alternative specifications of money laundering proxies, and to the inclusion of a number of firm-level and state-level control variables. We also conduct two-stage least squares and propensity score matching analysis to mitigate the endogeneity problem that might arise from omitted variables, reverse causality, or model misspecification problems.
Novel therapies for the management of type 2 diabetes mellitus: Part 1. Pramlintide and bromocriptine‐QR (2型糖尿病治疗新药：第1部分.普兰林肽与溴隐亭‐QR)

Several classes of antidiabetic agents have been introduced into the market place over the past dozen years. As our understanding of the underlying pathophysiology of type 2 diabetes has advanced, attempts have been made to address these defects specifically. This brief review focuses on our experience with two such pharmacological approaches: (i) a synthetic amylin analog addressing amylin deficiency; and (ii) a dopaminergic agonist, focused on enhancing the lowered dopaminergic tone in patients with type 2 diabetes. Importantly, the use of these agents is not associated with hypoglycemia or weight gain.
Domains of Crm1 involved in the formation of the Crm1, RanGTP, and leucine-rich nuclear export sequences trimeric complex.

Nuclear export of proteins containing a leucine-rich nuclear export sequence (NES) is mediated by a specific NES receptor known as Crm1. This protein, which is related to the karyopherin beta family, interacts directly with NES in a RanGTP-dependent manner. To characterize the domains of Crm1 involved in formation of the trimeric Crm1-NES-RanGTP complex, N- and C-terminal deletion mutants of Crm1 were generated and their ability to bind NES and RanGTP in vitro was analyzed. Our results indicate that two regions of Crm1 are required for the formation of the trimeric Crm1-NES-RanGTP complex, the N-terminal domain of Crm1 and the central domain of the receptor, starting after residue 160 with an essential region between 566 and 720. The N-terminal domain is homologous to the RanGTP-binding domain of karyopherin beta and therefore is likely involved in the interaction with RanGTP. Consequently, the central domain likely corresponds to the NES-binding site of Crm1.
Assessment of pulpal vitality using laser speckle imaging

The pulpal chamber of each tooth contains the vasculature necessary to maintain a viable tooth. A critical need exists to develop an objective, repeatable method to assess pulpal viability. We hypothesized that the existence of blood perfusion within the pulp can be determined with analysis of laser speckle imaging (LSI) patterns generated by transillumination of the tooth.
Platelet storage solution effects on the accuracy of laboratory tests for platelet function: a multi‐laboratory study

Background and Objectives Extent of shape change (ESC) and hypotonic shock response (HSR) have been widely used to characterize the in vitro function of platelets and have been shown to correlate with in vivo viability. These assays have been routinely performed using platelet‐poor plasma (PPP) as the test sample diluent. Because of the increasing popularity of storing platelets in synthetic media, it is important to understand the effects of using these synthetic media as test diluents for ESC and HSR measurements. The objective of this study was to determine the effect of using platelet storage solutions vs. plasma for the in vitro testing of ESC and HSR.
The autoinhibitory C-terminal SH2 domain of phospholipase C–γ2 stabilizes B cell receptor signalosome assembly

The immune disorder PLAID is found to be the result of destabilization of B cell receptor complexes due to loss of an autoinhibitory domain in phospholipase C–γ2. A Positive Role for an Autoinhibitory Domain in B Cell Signaling Activation of B cells requires antigen-dependent stimulation of B cell receptors (BCRs), which become assembled into complexes that provide sustained signals. BCR stimulation causes the recruitment and activation of phospholipase C–γ2 (PLC-γ2), which results in Ca2+ mobilization. Although patients with the immune disorder PLAID have a variant PLC-γ2 that is devoid of an autoinhibitory domain, their B cells exhibit defective Ca2+ signaling and are dysregulated. Wang et al. found that B cells from PLAID patients failed to form stable BCR complexes and had defective activation of downstream effectors. Together, these data suggest that the autoinhibitory domain of PLC-γ2 is also required to stabilize clustered receptors for sustained signaling. The binding of antigen to the B cell receptor (BCR) stimulates the assembly of a signaling complex (signalosome) composed initially of the kinases Lyn, spleen tyrosine kinase (Syk), and Bruton’s tyrosine kinase (Btk), as well as the adaptor protein B cell linker (BLNK). Together, these proteins recruit and activate phospholipase C–γ2 (PLC-γ2), a critical effector that stimulates increases in intracellular Ca2+ and activates various signaling pathways downstream of the BCR. Individuals with one copy of a mutant PLCG2 gene, which encodes a variant PLC-γ2 that lacks the autoinhibitory C-terminal Src homology 2 (cSH2) domain, exhibit PLC-γ2–associated antibody deficiency and immune dysregulation (PLAID). Paradoxically, although COS-7 cells expressing the variant PLC-γ2 show enhanced basal and stimulated PLC-γ2 activity, B cells from PLAID patients show defective intracellular Ca2+ responses upon cross-linking of the BCR. We found that the cSH2 domain of PLC-γ2 played a critical role in stabilizing the early signaling complex that is stimulated by BCR cross-linking. In the presence of the variant PLC-γ2, Syk, Btk, and BLNK were only weakly phosphorylated and failed to stably associate with the BCR. Thus, BCRs could not form stable clusters, resulting in dysregulation of downstream signaling and trafficking of the BCR. Thus, the cSH2 domain functions not only to inhibit the active site of PLC-γ2 but also to directly or indirectly stabilize the early BCR signaling complex.
Cloning and expression of fumarylacetoacetate hydrolase derived from marine yeast Rhodosporidium diobovatum

Fumarylacetoacetate hydrolase (FAH) catalyzes the hydrolytic cleavage of a carbon–carbon bond in fumarylacetoacetate to yield acetoacetate and fumarate as the final step in tyrosine degradation. In this study, the FAH genomic DNA and cDNA of Rhodosporidium. diobovatum were cloned by using the methods of degenerate polymerase chain reaction and RACE method. The results showed that the gene contained six exons, and that the encoded polypeptide held a sequence of 308 amino acid residues, containing a 75 residue N‐terminal prosequence, a 216 residue proteinase domain, and a 17 residue C‐terminal domain. Then, the expression vectors pET28a‐FAH were constructed and expressed in Escherichia coli. The enzymatic activity of FAH protein was determined by monitoring the decrease in optical density at 330 nm of the substrate fumarylacetoacetate. The values for Km and Vmax of the purified enzyme was 1.4 µM and 2.3 µmol · min−1 · mg−1, respectively.