Miracle-dz/UniFAR-FTUs huggingface dataset:
Construction of a complete rabbit cornea substitute using a fibrin-agarose scaffold.

PURPOSE To construct a full-thickness biological substitute of the rabbit cornea by tissue engineering. METHODS Ten rabbit corneas were surgically excised, and the three main cell types of the cornea (epithelial, stromal, and endothelial cells) were cultured. Genetic profiling of the cultured cells was performed by RT-PCR for the genes COL8 and KRT12. To develop an organotypic rabbit cornea equivalent, we used a sequential culture technique on porous culture inserts. First, endothelial cells were seeded on the base of the inserts. Then, a stroma substitute made of cultured keratocytes entrapped in a gel of human fibrin and 0.1% agarose was developed. Finally, cultured corneal epithelial cells were grown on the surface of the scaffold. Stratification of the epithelial cell layer was promoted by using an air-liquid culture technique. Corneal substitutes were analyzed by light and electron microscopy. RESULTS All three types of corneal cells were efficiently cultured in the laboratory, expanded, and used to construct a full-thickness cornea substitute. Gene expression analyses confirmed that cultured endothelial cells expressed the COL8 gene, whereas epithelial cells expressed KRT12. Microscopic evaluation of the cornea substitutes demonstrated that epithelial cells tended to form a normal stratified layer and that stromal keratocytes proliferated rapidly in the stromal substitute. The endothelial monolayer exhibited a pattern similar to a normal corneal endothelium. CONCLUSIONS These findings suggest that development of a full-thickness rabbit cornea model is possible in the laboratory and may open new avenues for research.
Speech-Language Services in Public Schools: How Policy Ambiguity Regarding Eligibility Criteria Impacts Speech-Language Pathologists in a Litigious and Resource Constrained Environment.

Public school districts must determine which students are eligible to receive special education and related services under the Individuals with Disabilities Education Act (IDEA). This study, which involves 39 interviews with speech-language pathologists and school administrators, examines how eligibility recommendations are made for one widely provided service: speechlanguage therapy. A key finding of this study is that the policy infrastructure guiding eligibility decisions has areas of significant ambiguity leading SLPs to face uncertainty about who should be found eligible for speech-language services. This ambiguity in policy opens the door for economic and legal factors to detrimentally influence the eligibility determination process resulting in high numbers of eligible students and correspondingly large SLP caseloads. Specifically, the litigious environment in which school districts operate puts SLPs on the defensive in the eligibility determination process. Further, speech-language therapy is increasingly utilized as a safety net given the lack of other resources available to academically struggling public school students. Finally, SLPs receive little administrative support or supervision further exacerbating their vulnerability to external pressures when making eligibility decisions. While focusing specifically on the eligibility decision process for speech-language services, this paper highlights issues that are likely applicable to how eligibility decisions for special education services are made more broadly. Speech-Language Services in Public Schools: How Policy Ambiguity Regarding Eligibility Criteria Impacts Speech-Language Pathologists in a Litigious and Resource Constrained Environment What are the challenges faced by school-based teams charged with making special education eligibility decisions? Who gets services, who doesn't and how do we decide? Do the decisions result in an effective allocation of limited resources? This paper explores these critical by questions by exploring how eligibility decisions are made with respect to a service provided to over half of all students receiving any form of special education services: speech-language services. Nationally, speech-language pathologists (SLPs) provide services to approximately 1.2 million students identified as “speech-language impairment” under IDEA (Office of Special Education Programs, 2011) as well as to another approximately 2.4 million students with primary disabilities other than SLI (e.g., autism, specific learning disability, etc.) (American
Influence of alcohol consumption on oxidative stress and antioxidant status in cancer patients--case-control study from Western Nepal.

AIM The present study assess the effect of consumption of alcohol on oxidative stress and antioxidant status in patients suffering from different types of cancer. METHODS This hospital based case control study conducted in the Western part of Nepal covered a total of 93 cancer patients with or without alcohol intake and smoking habits, along with 94 age, sex and habit-matched individuals serving as controls. Plasma thiobarbituric acid reacting substances (TBARS), total antioxidant activity (TAA), vitamin C, α-tocopherol and erythrocyte reduced glutathione (GSH) were estimated and compared. RESULTS The TBARS level was found to be significantly higher (p≤0.001) in all types of cancer patients when compared to controls, being aggravated in alcoholics with a smoking habit. No statistical significance (p≥0.05) was observed in the level of vitamin C and α-tocopherol. GSH and TAA level were significantly decreased (p≤0.001) in all the groups except those who consumed both branded as well as homemade alcohol and non-alcoholics without smoking habit. CONCLUSION Alcohol, irrespective of its commercial brand, increases oxidative stress in all types of cancer patients. This is even higher when alcohol intake is combined with a smoking habit. Decreased TAA and GSH are major risk factors for cancer development.
The pattern of slow wave activity in spontaneously occurring long sleep

SUMMARY The aim of the present study was to estimate the time course of slow wave activity (SWA) in naturally occurring long sleep episodes (ad lib). Sixteen male shift workers were subjected to 24 h ambulatory polysomnography in connection with an afternoon shift. The EEG was subjected to spectral analysis (FFT) as well as to traditional sleep stage scoring. SWA (0.5‐4.5 Hz band, both nonREM and REM sleep) declined exponentially and reached an asymptote by the fifth or sixth sleep cycle. However, half the subjects showed a reduced SWA in the first cycle, with a subsequent recovery in the second cycle. The SWA reduction of the first cycle was associated with a reduced REM‐latency and it was suggested that uncontrolled external influences of the real life settings may have affected SWA in the first cycle. It was concluded that the decline of SWA across time may deviate from an exponential shape under real life conditions.
Comparison of Bio-Rad %CDT TIA and CDTect as laboratory markers of heavy alcohol use and their relationships with gamma-glutamyltransferase.

BACKGROUND Carbohydrate-deficient transferrin (CDT) is used as a serum marker for heavy drinking. We compared a new Bio-Rad %CDT TIA assay with the CDTect assay; we also compared both to gamma-glutamyltransferase (GGT) as markers of heavy drinking. METHODS Serum samples of well-defined alcoholics (n = 404) and matched (age, race, and gender) social drinkers (204) from 10 clinical centers were assayed with both CDT assays. Both assays use microcolumn separation after iron saturation, followed by enzyme immunoassay (CDTect) or turbidimetric immunoassay (Bio-Rad %CDT). In the latter, CDT is expressed as a percentage of total transferrin. RESULTS The slope and intercept [95% confidence intervals (CIs)] for linear regression of results obtained by the %CDT-TIA (as percentage) and CDTect (units/L) assays were 0.091 (0.088-0.097) and 0.70% (0.54-0.86%), respectively (S(y/x) =1.30%; r = 0.848). The areas under the ROC curves (95% CIs) for CDTect and Bio-Rad %CDT TIA were 0.89 (0.86-0.92) and 0.88 (0.85-0.91), respectively, for men (P, not significant) and 0.76 (0.72-0.80) and 0.72 (0.68-0.76) for women (P, not significant). When CDT (CDTect or Bio-Rad %CDT) was combined with GGT (either one positive), the clinical sensitivity in men was 90% for both assays, and specificities were 81% and 84%, respectively; sensitivities in women were 75% and 76%, respectively, and specificities were 87% and 91%. CONCLUSION The new Bio-Rad %CDT TIA assay compares favorably to the widely studied CDTect assay in the detection of alcohol-use disorders.
The anti-CD20 monoclonal antibody rituximab to treat acquired haemophilia A.

BACKGROUND Acquired haemophilia A (AHA) is a rare bleeding disorder caused by the development of specific autoantibodies against naturally occurring factor VIII (FVIII). Although about half of cases are idiopathic, AHA may be associated with several non-neoplastic conditions, autoimmune disorders, as well as haematological malignancies, such as chronic lymphocytic leukaemia and lymphoma. The long-term suppression of inhibitors is one of the mainstays of the treatment of AHA. Apart from standard immunosuppressive treatments, rituximab has been proven to be effective in AHA. MATERIALS AND METHODS The aim of this review is to provide a systematic description of data available in the literature on this topic. To do so, we performed a search using the indexed online database Medline/PubMed, without temporal limits, matching the words "rituximab" and "acquired h(a)emophilia". Furthermore, additional published studies were identified in the reference list of the publications found in PubMed. RESULTS The review of the literature confirms that rituximab may be a safe and useful treatment for AHA. DISCUSSION Although rituximab is not a standard therapy for AHA, it may be useful in resistant cases. However, the definitive place of this monoclonal antibody in the therapeutic strategy for AHA (first or second-line, alone or in combination with other drugs) remains to be determined more precisely and warrants further investigation.
In Vitro and Ex Vivo Permeation Studies of Etodolac from Hydrophilic Gels and Effect of Terpenes as Enhancers

Etodolac, a highly lipophilic anti-inflammatory drug, is widely used in rheumatoid arthritis usually at an oral dose of 200 mg twice daily. The commonest side effects during therapy with etodolac is generally gastrointestinal disturbances these are usually mild and reversible but in some patients are peptic ulcer and severe gastrointestinal bleeding. To eliminate these side effects and obtain high drug concentration at the application side, dermal application of etodolac seems to be an ideal route for administration. Hydrophilic gel formulations of etodolac were prepared with carboxymethylcellulose sodium. The effect of different terpenes (anethole, carvacrol, and menthol) as an enhancer on the percutaneous absorption of etodolac was also investigated. Permeation studies were carried out with unjacketed modified horizontal diffusion cells through cellulose membrane and rat skin. In vitro studies with cellulose membrane showed that all formulations presented the same drug release profile (p > 0.05). Ex vivo studies with excised rat skin revealed that etodolac was released and penetrated into rat skin quickly. Anethole, a hydrophobic terpene, enhanced the absorption of etodolac significantly (p < 0.05). This result is consistent with the fact that hydrophobic terpenes are effective on the percutaneous absorption of lipophilic drugs. Menthol and carvacrol, hydrophilic terpenes, did not enhance the absorption of etodolac. The lipophilicity of the enhancers seems an important factor in promoting penetration of etodolac through the skin. Since etodolac creates gastrointestinal disturbances, topical formulations of etodolac in gel form including 1% anethole could be an alternative.
Characterization of Lead-Free Solder and Sintered Nano-Silver Die-Attach Layers Using Thermal Impedance

Since a die-attach layer has a significant impact on the thermal performance of a power module, its quality can be characterized using thermal performance. In this paper, a measurement system for thermal impedance is developed to evaluate three die-attach materials. Thanks to its high temperature sensitivity (10 mV/°C), the gate-emitter voltage of an insulated gate bipolar transistor (IGBT) is used as the temperature-sensitive parameter. The power dissipation in the IGBT remains constant by a feedback loop, regardless of the junction temperature. Experimental results show that the sample using sintered nano-silver for the die-attach has 12.1% lower thermal impedance than the samples using SAC305 and SN100C solders. To check the degradation of the die-attachment, six samples using three die-attach materials were thermally cycled from -40 to 125°C. The experimental results show that, after 500 cycles, the thermal impedance of SAC305 samples and SN100C samples is increased by 12.9% and 13.3%, respectively, which are much higher than that of the sample using the sintered nano-silver for the die-attach (3.1%).
Body Composition in Division I Football Players

This study assessed body composition of Division I football players (n = 69) and compared the findings with previously reported data to ascertain whether the increase in player total body mass that has been observed over the past 10 years has been accompanied by an increase in body fat. Body composition was determined by hydrostatic weighing and the measurement of skinfold thicknesses. Total body mass, skin-fold thicknesses, and body fat were greater in the current players than in players in studies conducted in the early 1980s and early 1990s. Body fat varied significantly across playing position, with the defensive backs, offensive backs, and receivers being the leanest and the offensive linemen and tight ends the most fat. There was no significant relationship between body composition and playing year or scholarship status, nor were any differences observed between ethnic groups. Of important clinical relevance was the finding that the linemen (offensive, defensive) and tight ends were on average greater than 25% body fat, the borderline for obesity in this age group. Much of this fat was deposited in the abdominal region, a significant finding when one considers the high correlation between abdominal obesity and ischemic heart disease and stroke. The current findings suggest that more attention needs to be given to the nature of the increase in body mass being achieved by today's football player to minimize long-term negative health consequences, and the findings reemphasize the need identified in earlier studies of the importance of detraining programs for these athletes.
Applications of symbolic computation to power system analysis and teaching

This paper describes the history and use of symbolic computation in power systems, from the tools used for power system analysis such as power flows, to its application in the classroom to facilitate the explanation and understanding of complex models and concepts such as device modeling and simulation. An example of the use of symbolic computation to develop a basic power flow program with the help of MATLAB/sup /spl reg//, and to explain the advantages and disadvantages of using different types of Jacobians in the solution process is discussed in detail.
Assessing the Value of the (New) Turkish Lira

Observing that the Turkish Lira has appreciated against the US Dollar in the recent years in spite of the higher inflation rate in Turkey brings a question about the applicability of PPP to Turkish case. We provide Turkish Balance of Payments, an indicator of funds flow, as an explanatory factor for the changes in the YTL/USD exchange rate. Both models used, with Balance of Payments and without, reveals that PPP does not hold between Turkey and the US for the time period examined.
The Integration of Law into Global Business Solutions: The Rise, Transformation, and Potential Future of the Big Four Accountancy Networks in the Global Legal Services Market

Using a unique data set comprised of original research of both the corporate Web sites of the Big Four—PwC, Deloitte, KPMG, and EY—and their affiliated law firms, as well as archival material from the legal and accountancy press, this article documents the rise and transformation of the Big Four legal service lines since the enactment of the Sarbanes Oxley Act of 2002. Moreover, it demonstrates that there are good reasons to believe that these sophisticated players will be even more successful in penetrating the corporate legal services market in the decades to come, as that market increasingly matures in a direction that favors the integration of law into a wider category of business solutions that these globally integrated multidisciplinary practices now champion. We conclude with some preliminary observations about the implications of the reemergence of the Big Four legal networks for the legal profession.
[Cloning, sequencing and expression of the full-length gene encoding paramyosin of Schistosoma japonicum in vivo].

AIM To clone and sequence the gene encoding paramyosin of S. japonicum (Chinese strain) and to study the expression of the DNA-based vaccine encoding the full-length paramyosin of S. japonicum in vivo. METHODS Total RNA was isolated from adult S. japonicum using TRIzol reagent. The full-length cDNA encoding paramyosin of S. japonicum was amplified by RT-PCR and cloned into pGEM-T vector and sequenced by the method of dideoxy-mediated chain-termination. The cDNA encoding paramyosin of S. japonicum was subcloned into the expressive plasmid vector pCDNA/AMP(pCMV-Sjc97), and the recombinants were identified by restriction enzyme digestion and sequencing. The immunofluorescence assay was used to study the expression of Sjc97 in vivo in mice. RESULTS AND CONCLUSION The 2.6 kb cDNA encoding the full-length paramyosin of Chinese S. japonicum has been successfully cloned and sequenced for the first time. The full-length sequence of paramyosin of S. japonicum was determined. Comparison of the nucleotide sequence and the deduced amino acid sequence of Sjc97 with that of S. japonicum paramyosin (Philippine strain) (Sjp97), S. japonicum paramyosin(Japanese strain) (Sjj97), S. mansoni (Sm97), B6 and Y6 clone (the partial cDNA encoding paramyosin of Chinese strain) showed that Sjc97 differed from Sjp97 by 16/2,601 nucleotide and 3/866 amino acid substitutions (99.4% on nt-level and 99.7% on aa-level in homology); from Sjj97 by 20/2,601 nucleotide and 2/866 amino acid substitutions (99.2% on nt-level and 99.8% on aa-level in homology); and from B6 by 11/1,329 nucleotide and 1/443 amino acid substitutions (99.0% on nt-level and 99.8% on aa-level in homology); from Y6 by 13/1,329 nucleotide and 1/443 amino acid substitutions (98.9% on nt-level and 99.8% on aa-level in homology); Sjc97 differed from the Sm97 by 2,235/2,601 nucleotide and 34/866 amino acid (91.0% on nt-level and 96.0% on aa-level in homology). The plasmid expression vector encoding the full-length paramyosin of Chinese S. japonicum has been successfully constructed. The pCMV-Sjc97 vaccine could express Sjc97 protein in vivo in mice after intramuscular immunization.
Quantitation of proteoglycans as glycosaminoglycans in biological fluids using an alcian blue dot blot analysis.

A method for quantitation of intact proteoglycans as GAGs in biological fluids (blood plasma, synovial fluid) or 4 M guanidine extracts of tissues has been published previously (S. Björnsson, Anal. Biochem. 210, 282-291, 1993). The method is based on the specific interaction between sulfated polymers and the tetravalent cationic dye Alcian blue at pH 1.5 in 0.4 M guanidine-HCl and in the presence of 0.25% Triton. The absorbance assay has a measuring range of 1-20 microgram of glycosaminoglycan (GAG) which is not sensitive enough to measure the low contents of proteoglycans in blood plasma, urine, or wound fluid. A dot blot assay is now described in which the Alcian blue-GAG complexes are collected on a polyvinylidene fluoride membrane, by filtration in a dot blot apparatus, and the stain is quantitated as reflectance by scanning and densitometry. The assay requires 10 microliter of sample and has a measuring range of 10-800 ng of GAG, corresponding to a concentration of 1-80 mg/liter, suitable for proteoglycans in biological fluids. The procedures for chemistry, scanning, densitometry, and curve fitting were each evaluated separately. The error contributed by chemistry accounted for a minor portion of the imprecision. The imprecision contributed by scanning was the most important source of within-run and between-run imprecision, and was caused by inequalities of the charge-coupled device along the scanning arm. Unexpectedly, curve fitting was also a major source of total imprecision in dot blot quantitation and differed with the type of equation used. The between-run imprecision calculated as CV (SD/mean . 100) was 13.0% at 8 mg/liter. The response of the assay was identical for six different commercial preparations of GAGs (chondroitin-4-sulfate, chondroitin-6-sulfate, dermatan sulfate, keratan sulfate, heparan sulfate, and heparin) despite differences in degree of sulfation known to exist. There was no positive or negative interference by blood plasma, apart from a slight negative interference on the quantitation of heparan sulfate. Analysis of 319 paired blood plasma and urine specimens from hospitalized patients showed a variation of plasma GAGs of 0.1-17.6 and urine-GAGs of 0.0-45.6 mg/liter. There was no correlation between plasma and urine GAG concentrations.
Trends, Issues and Challenges in Internationalisation of Higher Education

In a context of economic crisis and increased anti immigration politics, international students and skilled immigration are high on the political and educational agenda in The Netherlands as well as other European countries. There is an increasing tension between short term anti-immigrant tendencies and budget cuts for research and development on the one hand, and the long term need for skilled immigration to stay competitive in the global knowledge economy on the other hand. In the past, rationales for attracting international students were either trade, aid or cooperation, global competition for highly skilled manpower has become a strong new pull factor in international student circulation, and policies are developed by The Netherlands and other European countries to stay competitive.