Miracle-dz/UniFAR-FTUs huggingface dataset:
Connexin26 in adult rodent central nervous system: Demonstration at astrocytic gap junctions and colocalization with connexin30 and connexin43

The connexin family of proteins (Cx) that form intercellular gap junctions in vertebrates is well represented in the mammalian central nervous system. Among these, Cx30 and Cx43 are present in gap junctions of astrocytes. Cx32 is expressed by oligodendrocytes and is present in heterologous gap junctions between oligodendrocytes and astrocytes as well as at autologous gap junctions between successive myelin layers. Cx36 mRNA has been identified in neurons, and Cx36 protein has been localized at ultrastructurally defined interneuronal gap junctions. Cx26 is also expressed in the CNS, primarily in the leptomeningeal linings, but is also reported in astrocytes and in neurons of developing brain and spinal cord. To establish further the regional, cellular, and subcellular localization of Cx26 in neural tissue, we investigated this connexin in adult mouse brain and in rat brain and spinal cord using biochemical and immunocytochemical methods. Northern blotting, western blotting, and immunofluorescence studies indicated widespread and heterogeneous Cx26 expression in numerous subcortical areas of both species. By confocal microscopy, Cx26 was colocalized with both Cx30 and Cx43 in leptomeninges as well as along blood vessels in cortical and subcortical structures. It was also localized at the surface of oligodendrocyte cell bodies, where it was coassociated with Cx32. Freeze‐fracture replica immunogold labeling (FRIL) demonstrated Cx26 in most gap junctions between cells of the pia mater by postnatal day 4. By postnatal day 18 and thereafter, Cx26 was present at gap junctions between astrocytes and in the astrocyte side of most gap junctions between astrocytes and oligodendrocytes. In perinatal spinal cord and in five regions of adult brain and spinal cord examined by FRIL, no evidence was obtained for the presence of Cx26 in neuronal gap junctions. In addition to its established localization in leptomeningeal gap junctions, these results identify Cx26 as a third connexin (together with Cx30 and Cx43) within astrocytic gap junctions and suggest a further level of complexity to the heterotypic connexin channel combinations formed at these junctions. J. Comp. Neurol. 441:302–323, 2001. © 2001 Wiley‐Liss, Inc.
Pertussis toxin transiently affects barrier integrity, organelle organization and transmigration of monocytes in a human brain microvascular endothelial cell barrier model

Encephalopathies and neurological disorders are sometimes associated with respiratory tract infections caused by Bordetella pertussis. For these complications to occur cerebral barriers have to be compromised. Therefore, the influence of pertussis toxin (PT), a decisive virulence determinant of B. pertussis, on endothelial barrier integrity was investigated. Human brain microvascular endothelial cells cultured on Transwell filter devices were used as model for the blood brain barrier. PT, but not its B‐oligomer, induced a reduction of the transendothelial resistance and enhanced the permeability for the protein marker horseradish peroxidase. Moreover, transmigration of human monocytes was also elevated suggesting a PT‐associated enhancement of the diapedesis of blood leucocytes. Uptake and trafficking of PT was followed by electron microscopy via clathrin‐coated pits and accumulation in lysosomes and microvesicular bodies. The breach in barrier integrity was accompanied by a transient disintegration of Golgi structures. Interestingly, PT‐induced effects were only transient and restoration of barrier function was observed after 24 h. In summary, intoxication by PT causes a transient destruction of the cellular organization in human brain‐derived endothelial cells resulting in a transient disruption of barrier functions. We suggest that these findings reflect early steps in the development of neurological disorders associated with pertussis disease.
Data Migration for aWidely Distributed Storage System Using Autonomous Disks

We propose a data migration method for widely distributed autonomous disks, in which user’s frequently accessed and important data are automatically transferred to the nearest disk and/or duplicated to others by using ECA rules, according to the properties of the data. Recently, a mobile user can handle a large amount of data because mobile disks are installed on many gadgets. However, data required by a user are not always on his mobile disk. Even if they are on it, the reliability is low due to the possibility of a disk crash. The proposed system makes use of multiple sets of ECA rules on autonomous disks and chooses an appropriate rule set in response to the changes of system states, so that optimal data accesses can be performed by migrating and/or duplicating the data to other disks. This system could bring comfortable and reliable data accesses to mobile users.
An Analysis of the Relationship Between Learning Style Perceptual Preferences and Attitudes Toward Computer-Assisted Instruction

This Dissertation is brought to you for free and open access. It has been accepted for inclusion in Dissertations and Theses by an authorized administrator of PDXScholar. For more information, please contact pdxscholar@pdx.edu. Recommended Citation McFarland, Mary Brambilla, "An Analysis of the Relationship Between Learning Style Perceptual Preferences and Attitudes Toward Computer-Assisted Instruction" (1989). Dissertations and Theses. Paper 1229.
Tract-Based Spatial Statistical Analysis of Diffusion Tensor Imaging in Pediatric Patients with Mitochondrial Disease: Widespread Reduction in Fractional Anisotropy of White Matter Tracts

BACKGROUND AND PURPOSE: Often diagnosed at birth or in early childhood, mitochondrial disease presents with a variety of clinical symptoms, particularly in organs and tissues that require high energetic demand such as brain, heart, liver, and skeletal muscles. In a group of pediatric patients identified as having complex I or I/III deficits on muscle biopsy but with white matter tissue appearing qualitatively normal for age, we hypothesized that quantitative DTI analyses might unmask disturbance in microstructural integrity. MATERIALS AND METHODS: In a retrospective study, DTI and structural MR brain imaging data from 10 pediatric patients with confirmed mitochondrial disease and 10 clinical control subjects were matched for age, sex, scanning parameters, and date of examination. Paired TBSS was performed to evaluate differences in FA, MD, and the separate diffusion direction terms (λr and λa). RESULTS: In patients with mitochondrial disease, significant widespread reductions in FA values were shown in white matter tracts. Mean diffusivity values were significantly increased in patients, having a sparser distribution of affected regions compared with FA. Separate diffusion maps showed significant increase in λr and no significant changes in λa. CONCLUSIONS: Despite qualitatively normal-appearing white matter tissues, patients with complex I or I/III deficiency have widespread microstructural changes measurable with quantitative DTI.
Reductions in Platelet Force Development by Cardiopulmonary Bypass Are Associated with Hemorrhage

Quantitative assessment of platelet dysfunction after cardiopulmonary bypass (CPB) and prediction of excessive microvascular bleeding remain elusive goals.We used a sensitive instrument capable of simultaneously measuring the force generated by platelets during plasma clot retraction and global clot strength. We hypothesized that CPB would significantly reduce these two variables. Platelet-rich plasma was obtained from eight patients undergoing aortocoronary revascularization prior to induction, after 90 min of CPB, and after protamine administration. Platelet force development was measured using a standardized technique that controlled for platelet number and permitted clot formation in the presence of heparin. Despite the presence of a measurable elastic modulus, platelet force development during bypass was abolished. Peak platelet force development after CPB was significantly lower than before CPB (5255 +/- 955 dynes vs 11,600 +/- 780 dynes, P = 0.01). The percent recovery (after/before bypass) of peak platelet force development inversely correlated with tube thoracostomy drainage during the first 24 h after placement (rs = -0.71, P = 0.048). This study demonstrates that CPB has dramatic effects on platelet force development. The correlation between the percent recovery of peak platelet force development and blood loss supports the clinical relevance of this measurement. (Anesth Analg 1995;80:459-65)
Bulk of interest: performance measurement of content-centric routing

The paradigm of information-centric networking subsumes recent approaches to integrate content replication services into a future Internet layer. Current concepts foster either a dynamic mapping that directs content requests to a nearby copy, or an immediate routing on content identifiers. In this paper, we evaluate in practical experiments the performance of content routing, which we analyze with a focus on conceptual aspects. Our findings indicate that the performance of the content distribution system is threatened by a heavy management of states that arise from the strong coupling of the control to the data plane in the underlying routing infrastructure.
RELIABILITY OF THE ICD-10 INTERNATIONAL PERSONALITY DISORDER EXAMINATION (IPDE) (HINDI VERSION): A PRELIMINARY STUDY

The study was aimed at estimating the joint-rater reliability and applicability of Hindi version of ICD-10 IPDE that was obtained following a standard translation protocol. The instrument was administered to 22 non-psychotic patients by two raters. The average intraclass correlation for each item (0.89), number of criteria met per disorder (0.92) and dimensional scores (0.98) was high. Kappa for definite (0.65-0.78) and probaole personality disorder (PD) (0.78-1.00) and for presence/absence of any PD (0.78) was acceptable. Overall weighted kappa was 0.81 for definite and 0.91 for probable PD. Findings suggest that ICD-10 IPDE (Hindi version) has acceptable joint- rater reliability and applicability in the North Indian Hindi speaking population.
Acoustic Denial of Service Attacks on HDDs

Among storage components, hard disk drives (HDDs) have become the most commonly-used type of non-volatile storage due to their recent technological advances, including, enhanced energy efficacy and significantly-improved areal density. Such advances in HDDs have made them an inevitable part of numerous computing systems, including, personal computers, closed-circuit television (CCTV) systems, medical bedside monitors, and automated teller machines (ATMs). Despite the widespread use of HDDs and their critical role in real-world systems, there exist only a few research studies on the security of HDDs. In particular, prior research studies have discussed how HDDs can potentially leak critical private information through acoustic or electromagnetic emanations. Borrowing theoretical principles from acoustics and mechanics, we propose a novel denial-of-service (DoS) attack against HDDs that exploits a physical phenomenon, known as acoustic resonance. We perform a comprehensive examination of physical characteristics of several HDDs and create acoustic signals that cause significant vibrations in HDD's internal components. We demonstrate that such vibrations can negatively influence the performance of HDDs embedded in real-world systems. We show the feasibility of the proposed attack in two real-world case studies, namely, personal computers and CCTVs.
Integration of Groundwater Transport Models with Wireless Sensor Networks

Groundwater transport modeling is intended to aid in remediation processes by providing prediction of plume behavior. Wireless Sensor Networks (WSNs), an emerging technology, allows integration of data from multiple sensors to form an integrated, distributed network that monitors dynamic hydrological and environmental processes. As the wireless nodes are resource constrained, mass transport predictions may be used to increase the efficiency of the application-driven WSN protocols. A symbiotic relationship then exists between the WSN, which supplies data to calibrate the transport model in real-time, and the model, which optimizes the WSN monitoring performance. As current model calibration methods often require manual adjustment of calibration parameters, real-time calibration procedures pose significant challenges. Based on insights from a previous intermediate-scale, proof-of-concept study, we are developing the models, tools, and protocols necessary for a closed-loop simulation. The results presented here address the setup of a WSN simulator which cooperates with transport models, development of fault detection techniques into the network protocols, and a preliminary discussion of how transport models may be conceptualized in the WSN context.
Modulation of Untruthful Responses with Non-Invasive Brain Stimulation

Deceptive abilities have long been studied in relation to personality traits. More recently, studies explored the neural substrates associated with deceptive skills suggesting a critical role of the prefrontal cortex. Here we investigated whether non-invasive brain stimulation over the dorsolateral prefrontal cortex (DLPFC) could modulate generation of untruthful responses about subject’s personal life across contexts (i.e., deceiving on guilt-free questions on daily activities; generating previously memorized lies about past experience; and producing spontaneous lies about past experience), as well as across modality responses (verbal and motor responses). Results reveal that real, but not sham, transcranial direct current stimulation (tDCS) over the DLPFC can reduce response latency for untruthful over truthful answers across contexts and modality responses. Also, contexts of lies seem to incur a different hemispheric laterality. These findings add up to previous studies demonstrating that it is possible to modulate some processes involved in generation of untruthful answers by applying non-invasive brain stimulation over the DLPFC and extend these findings by showing a differential hemispheric contribution of DLPFCs according to contexts.
Identification of Genes Directly Involved in Shell Formation and Their Functions in Pearl Oyster, Pinctada fucata

Mollusk shell formation is a fascinating aspect of biomineralization research. Shell matrix proteins play crucial roles in the control of calcium carbonate crystallization during shell formation in the pearl oyster, Pinctada fucata. Characterization of biomineralization-related genes during larval development could enhance our understanding of shell formation. Genes involved in shell biomineralization were isolated by constructing three suppression subtractive hybridization (SSH) libraries that represented genes expressed at key points during larval shell formation. A total of 2,923 ESTs from these libraries were sequenced and gave 990 unigenes. Unigenes coding for secreted proteins and proteins with tandem-arranged repeat units were screened in the three SSH libraries. A set of sequences coding for genes involved in shell formation was obtained. RT-PCR and in situ hybridization assays were carried out on five genes to investigate their spatial expression in several tissues, especially the mantle tissue. They all showed a different expression pattern from known biomineralization-related genes. Inhibition of the five genes by RNA interference resulted in different defects of the nacreous layer, indicating that they all were involved in aragonite crystallization. Intriguingly, one gene (UD_Cluster94.seq.Singlet1) was restricted to the ‘aragonitic line’. The current data has yielded for the first time, to our knowledge, a suite of biomineralization-related genes active during the developmental stages of P.fucata, five of which were responsible for nacreous layer formation. This provides a useful starting point for isolating new genes involved in shell formation. The effects of genes on the formation of the ‘aragonitic line’, and other areas of the nacreous layer, suggests a different control mechanism for aragonite crystallization initiation from that of mature aragonite growth.
Different culture methods lead to differences in glycosylation of a murine IgG monoclonal antibody.

A monoclonal IgG-1 was produced by culture of a murine hybridoma (3.8.6) by three different methods, namely culture in ascites, in serum-free media and in serum-supplemented media. IgG-1 was purified to homogeneity (as judged by SDS/PAGE under reducing conditions) from each medium by ion-exchange chromatography and h.p.l.c. Protein A chromatography. Oligosaccharides were released from each IgG-1 preparation by hydrazinolysis and radiolabelled by reduction with alkaline sodium borotritide, and 'profile' analysis of the radiolabelled oligosaccharide alditols was performed by a combination of paper electrophoresis and gel-filtration chromatography. This analysis indicated clear and reproducible differences in the glycosylation patterns of the three IgG-1 preparations. Sequential exoglycosidase analysis of individual oligosaccharides derived from each IgG-1 preparation was used to define these differences. Ascites-derived material differed from serum-free-culture-derived material only with respect to the content of sialic acid. IgG-1 derived from culture in serum-containing media had an intermediate sialic acid content and a lower incidence of outer-arm galactosylation than the other two preparations. These differences in glycosylation could not be induced in any IgG-1 preparation by incubating purified IgG-1 with ascites or culture medium. It is concluded that the glycosylation pattern of a secreted monoclonal IgG is dependent on the culture method employed to obtain it.
Boltzmann sampling of unlabelled structures

Boltzmann models from statistical physics combined with methods from analytic combinatorics give rise to efficient algorithms for the random generation of unlabelled objects. The resulting algorithms generate in an unbiased manner discrete configurations that may have nontrivial symmetries, and they do so by means of real-arithmetic computations. We present a collection of construction rules for such samplers, which applies to a wide variety of combinatorial classes, including integer partitions, necklaces, unlabelled functional graphs, dictionaries, series-parallel circuits, term trees and acyclic molecules obeying a variety of constraints, and so on. Under an abstract real-arithmetic computation model, the algorithms are, for many classical structures, of linear complexity provided a small tolerance is allowed on the size of the object drawn. As opposed to many of their discrete competitors, the resulting programs routinely make it possible to generate random objects of sizes in the range 104--106.
Accurate Partial Volume Estimation of MR Brain Tissues

Accurate estimation of tissue volume has important applications in brain diagnostic and morphologic studies. In this paper, we show how partial volume estimation of brain tissues from 3D MR brain images can be performed using the recently proposed adaptive spatial FCM algorithm. The efficacy of the proposed algorithm is demonstrated experimentally using simulated MR images with known ground truths.
Making friends with your data: improving how statistics are conducted and reported.

AIM This paper highlights some of the areas where there are problems with the way that statistics are conducted and reported in psychology journals. Recommendations are given for improving these problems. SAMPLE The choice of topics is based largely on the questions that authors, reviewers, and editors have asked in recent years. The focus is on null hypothesis significance testing (NHST), choosing a statistical test, and what should be included in results sections. RESULTS There are several ways to improve how statistics are reported. These should improve both the authors' and the readers' understanding of the data. CONCLUSIONS Psychology as a discipline will improve if the way in which statistics are conducted and reported is improved. This will require effort from authors, scrutiny from reviewers, and stubbornness from editors.
Your Second Selves: Resources, Agency, and Constraints in Avatar Designs and Identity Play in a Tween Virtual World

Avatars in online games and worlds are seen as players’ key representations in interactions with others. It is surprising then that this aspect of game play has not received much attention in research, in particular what concerns playergenerated avatars. In this paper, we investigate the avatar design and identity play within a large-scale tween virtual world called Whyville.net with more than 1.5 million registered players ages 8-16. One unique feature of Whyville is the player’s ability to customize one’s avatar with various face parts and accessories, all designed and sold by other players in Whyville. Our findings report on the expressive resources available for avatar construction, individual tween players’ choices and rationales in creating their avatars, and online postings about avatar design in the community at large. With the growing interest in playergenerated content for online worlds such as Second Life, our discussion will address the role of avatars in identity play and self-representation as well as the social issues that arise within the game world.
Does a fall prevention educational programme improve knowledge and change exercise prescribing behaviour in health and exercise professionals? A study protocol for a randomised controlled trial

Introduction Falling in older age is a serious and costly problem. At least one in three older people fall annually. Although exercise is recognised as an effective fall prevention intervention, low numbers of older people engage in suitable programmes. Health and exercise professionals play a crucial role in addressing fall risk in older adults. This trial aims to evaluate the effect of participation in a fall prevention educational programme, compared with a wait-list control group, on health and exercise professionals’ knowledge about fall prevention and the effect on fall prevention exercise prescription behaviour and confidence to prescribe the exercises to older people. Methods and analysis A randomised controlled trial involving 220 consenting health and exercise professionals will be conducted. Participants will be individually randomised to an intervention group (n=110) to receive an educational workshop plus access to internet-based support resources, or a wait-list control group (n=110). The two primary outcomes, measured 3 months after randomisation, are: (1) knowledge about fall prevention and (2) self-perceived change in fall prevention exercise prescription behaviour. Secondary outcomes include: (1) participants’ confidence to prescribe fall prevention exercises; (2) the proportion of people aged 60+ years seen by trial participants in the past month who were prescribed fall prevention exercise; and (3) the proportion of fall prevention exercises prescribed by participants to older people in the past month that comply with evidence-based guidelines. Outcomes will be measured with a self-report questionnaire designed specifically for the trial. Ethics and dissemination The trial protocol was approved by the Human Research Ethics Committee, The University of Sydney, Australia. Trial results will be disseminated via peer reviewed journals, presentations at international conferences and participants’ newsletters. Trial registration number Trial protocol was registered with the Australian and New Zealand Clinical Trials Registry (Number ACTRN12614000224628) on 3 March 2014.