Effect of Nutritionally Relevant Doses of Long-Chain N-3 Pufa on Lipid Status, Oxidative Stress and Inflammatory Markers in an Average Middle-Aged Serbian Population

Summary Background: This study investigated the effects of a nutritionally relevant intake of eicosapentaenoic (EPA) and docosahexaenoic (DHA) fatty acids derived from oily fish or a fish oil supplement on selected cardiovascular risk factors in average middle-aged individuals. Methods: Thirty-three participants were randomized to receive salmon (oily fish) providing 274 mg EPA + 671 mg DHA/day or a commercial fish oil supplement providing 396 mg EPA + 250 mg DHA/day in a cross-over trial over an 8-week period separated by a 6-month washout period. Blood samples were collected before and after each intervention and lipids, inflammatory and oxidative stress parameters were determined. Results: Plasma levels of EPA, DHA and total n-3 fatty acids significantly increased after both interventions. A decreasing trend in triglycerides was more pronounced with salmon than with the fish oil supplement, but the changes noticed were not significant. Although there were no relevant changes in inflammatory marker concentrations at the end of both interventions, significant negative correlations were noticed between total plasma n-3 fatty acids and soluble intercellular adhesion molecule and Creactive proteinconcenthroughout the whole intervention period (p<0.05). Among the oxidative stress parameters, intervention with salmon showed a prooxidative effect through a superoxide anion increase (p=0.025). A relevant positive correlation was also found between its concentration and total plasma n-3 fatty acids (p<0.05). Other oxidative stress markers were not significantly influenced by the dietary interventions applied. Conclusion: Following two sets of recommendations for n- 3 fatty acids intake aimed at the general public had only a moderate effect on the selected cardiovascular risk factors in average healthy middle-aged subjects over a short-term period Kratok sadržaj Uvod: U ovoj studiji praćeni su efekti preporuka za unos eikozapentaenske (EPA) i dokozoheksaenske kiseline (DHA), iz dva razlicita izvora, lososa i suplementa sa ribljim uljem, na odabrane faktore kardiovaskularnog rizika u prosečnoj populaciji srednjih godina. Metode: Trideset i tri ispitanika su po slučajnom izboru konzumirali losos koji je obezbeđivao 274 mg EPA +671 mg DHA/dan ili komercijalni suplement ribljeg ulja koji je obezbeđivao dnevno 396 mg EPA + 250 mg DHA tokom 8 nedelja. Nakon perioda od 6 meseci ispitanicima su zamenjene intervencije (ukrštena studija). Uzorci krvi su sakupljani pre i posle svake intervencije, a zatim su određivani lipidni, inflamatorni parametri kao i markeri oksidativnog stresa. Rezultati: Koncentracije EPA, DHA i ukupnih n-3 masnih kiselina u plazmi su značajno povećane posle obe intervencije. Nije bilo statistički značajnnih promena u lipidnim parametrima, iako je zabelezeno vece smanjenje nivoa triglicerida posle intervencije lososom u poredenju sa suplementom. Nisu uočene značajne promene u koncentraciji inflamatornih markera, ali je utvrđena značajna negativna korelacija između ukupnih n-3 masnih kiselina plazme i rastvorljivog intracelularnog adhezionog molekula i C-reaktivnog proteina tokom ukupnog trajanja studije (p<0,05). Od parametara oksidativnog stresa, intervencija lososom je imala umeren prooksidativni efekat usled povecanja superoksidnog anjona (p= 0,025). Između ovog parametra i ukupnih n-3 masnih kiselina nađena je statistički značajna pozitivna korelacija. Ostali marked oksidativnog stresa nisu se značajno menjali. Zaključak: Preporučene vrednosti za unos n-3 masnih kiselina pokazale su umeren efekat na parametre kardiovaskularnog rizika u prosečnoj populaciji srednjih godina u kratkom periodu trajanja studije