Miracle-dz/UniFAR-FTUs huggingface dataset:
Managing Projects in a Games Factory: Temporality and Practices

The online gaming world is growing voraciously and so too is the wider market for computer games which now exceeds the annual global revenues of cinema. Even so, we have only a limited scholarly understanding of how games studios produce games. Games projects require particular attention because their context is unique. We aim to elucidate the games development process from a time perspective and suggest ways of managing it. We argue that a games project is characterised by a compound of temporal rhythms that emanate from the individual, team, company, industry, nation, inter-nation and marketplace levels. Project managers need to take account of these 'inner' and 'outer' temporal rhythms since they can affect development culture and project chronology.
Triatoma infestans Apyrases Belong to the 5′-Nucleotidase Family*

Apyrases are nucleoside triphosphate-diphosphohydrolases (EC 3.6.1.5) present in a variety of organisms. The apyrase activity found in the saliva of hematophagous insects is correlated with the prevention of ADP-induced platelet aggregation of the host during blood sucking. Purification of apyrase activity from the saliva of the triatomine bug Triatoma infestans was achieved by affinity chromatography on oligo(dT)-cellulose and gel filtration chromatography. The isolated fraction includes five N-glycosylated polypeptides of 88, 82, 79, 68 and 67 kDa apparent molecular masses. The isolated apyrase mixture completely inhibited aggregation of human blood platelets. Labeling with the ATP substrate analogue 5′-p-fluorosulfonylbenzoyladenosine showed that the five species have ATP-binding characteristic of functional apyrases. Furthermore, tandem mass spectroscopy peptide sequencing showed that the five species share sequence similarities with the apyrase from Aedes aegypti and with 5′-nucleotidases from other species. The complete cDNA of the 79-kDa enzyme was cloned, and its sequence confirmed that it encodes for an apyrase belonging to the 5′-nucleotidase family. The gene multiplication leading to the unusual salivary apyrase diversity in T. infestans could represent an important mechanism amplifying the enzyme expression during the insect evolution to hematophagy, in addition to an escape from the host immune response, thus enhancing acquisition of a meal by this triatomine vector of Chagas' disease.
SERUM ANTIGEN 85 LEVELS IN ADJUNCT TESTING FOR ACTIVE MYCOBACTERIAL INFECTIONS IN ORANGUTANS

Diagnosis of active mycobacterial disease in orangutans (Pongo pygmaeus) has been impeded by high levels of non-specific intradermal skin test reactivity to mycobacterial antigens. This may be due in part to cross reactivity between antigens, tuberculin concentrations used or other species-specific factors. Antigen 85 (Ag85) complex proteins are major secretory products of actively growing mycobacteria, and measurement of serum Ag85 could provide a method for determining active mycobacterial infections that was not dependent on host immunity. Serum Ag85 was measured by dot-immunobinding assay using monoclonal anti-Ag85, purified Ag85 standard and enhanced chemiluminescence technology in coded serum samples from 14 captive orangutans from a zoo in Colorado, 15 semi-captive orangutans in Malaysia, and 19 free-ranging wild orangutans in Malaysia. Orangutans from Colorado (USA) were culture negative for Mycobacterium tuberculosis and M. avium, although all had laboratory suspicion or evidence of mycobacterial infection; median serum Ag85 was 10 μU/ml (range, <0.25–630 μU/ml). Of the semi-captive orangutans, six were skin test reactive and two were culture positive for M. avium on necropsy. Median serum Ag85 for this group was 1,880 μU/ml (0.75–7,000 μU/ml), significantly higher than that of Colorado zoo or free-ranging Malaysian orangutans. Median serum Ag85 in the latter group was 125 μU/ml (range, 0.75–2,500 μU/ml). These data suggest that suggest that additional studies using more specific reagents and more samples from animals of known status are appropriate.
Two different mutants blocked in synthesis of dolichol-phosphoryl-mannose do not add glycophospholipid anchors to membrane proteins: quantitative correction of the phenotype of a CHO cell mutant with tunicamycin

The addition of glycophospholipid (GPL) anchors to certain membrane proteins occurs in the rough endoplasmic reticulum and is essential for transport of the proteins to the plasma membrane. Limited circumstantial evidence suggests that dolichol-phosphoryl-mannose (DPM) is a donor of mannose residues of these anchors. We here report studies of a CHO cell mutant (B421) transfected to express the GPL-anchored protein, placental alkaline phosphatase (AP). Only a few transfectants were found to express GPL-anchored AP on their surface, and these clones synthesized DPM. Moreover, and most strikingly, when surface AP-negative transfectants were treated with tunicamycin to cause accumulation of DPM, these cells expressed lipid-anchored AP. Fusion of a cloned surface AP-negative transfectant of B421 with the Thy-1-class E mutant thymoma, which is also deficient in DPM synthesis, produced hybrids that synthesized DPM and expressed AP and Thy-1. Thus, two mutations can interrupt DPM synthesis, and three sets of observations point to an essential role of DPM for addition of GPL anchors.
Determinant Factors Influencing the Quality of Financial Reporting Local Government in Indonesia

The purpose of this study to determine factors influencing the quality of financial reporting local government in Indonesia. The factor studied consist of commitment apparatus, role of internal audit, and accounting information system. The sample is picked up randomly by a random sample technique. The data collected is then tested for its validity and reliability so that the data is valid to be processed. The unit of analysis in this study is 66 unit tool of regency/city Indonesian local government . Hypothesis to be tested by a statistical t test: Ho is rejected if tcount > tcritical, ? = 0.05 level. The results showed that the commitment apparatus, role of internal audit have a significant effect on the quality of accounting information systems. Furthermore it was found that the quality of accounting information system has implications for the quality of financial reporting.
Gauge Equivariant Transformer

Attention mechanism has shown great performance and efﬁciency in a lot of deep learning models, in which relative position encoding plays a crucial role. However, when introducing attention to manifolds, there is no canonical local coordinate system to parameterize neighborhoods. To address this issue, we propose an equivariant transformer to make our model agnostic to the orientation of local coordinate systems ( i.e. , gauge equivariant), which employs multi-head self-attention to jointly incorporate both position-based and content-based information. To enhance expressive ability, we adopt regular ﬁeld of cyclic groups as feature ﬁelds in intermediate layers, and propose a novel method to parallel transport the feature vectors in these ﬁelds. In addition, we project the position vector of each point onto its local coordinate system to disentangle the orientation of the coordinate system in ambient space ( i.e. , global coordinate system), achieving rotation invariance. To the best of our knowledge, we are the ﬁrst to introduce gauge equivariance to self-attention, thus name our model Gauge Equivariant Transformer (GET), which can be efﬁciently implemented on triangle meshes. Extensive experiments show that GET achieves state-of-the-art performance on two common recognition tasks.
Antisense Oligonucleotide-Based Therapy for Neuromuscular Disease

Neuromuscular disorders such as Duchenne Muscular Dystrophy and Spinal Muscular Atrophy are neurodegenerative genetic diseases characterized primarily by muscle weakness and wasting. Until recently there were no effective therapies for these conditions, but antisense oligonucleotides, a new class of synthetic single stranded molecules of nucleic acids, have demonstrated promising experimental results and are at different stages of regulatory approval. The antisense oligonucleotides can modulate the protein expression via targeting hnRNAs or mRNAs and inducing interference with splicing, mRNA degradation, or arrest of translation, finally, resulting in rescue or reduction of the target protein expression. Different classes of antisense oligonucleotides are being tested in several clinical trials, and limitations of their clinical efficacy and toxicity have been reported for some of these compounds, while more encouraging results have supported the development of others. New generation antisense oligonucleotides are also being tested in preclinical models together with specific delivery systems that could allow some of the limitations of current antisense oligonucleotides to be overcome, to improve the cell penetration, to achieve more robust target engagement, and hopefully also be associated with acceptable toxicity. This review article describes the chemical properties and molecular mechanisms of action of the antisense oligonucleotides and the therapeutic implications these compounds have in neuromuscular diseases. Current strategies and carrier systems available for the oligonucleotides delivery will be also described to provide an overview on the past, present and future of these appealing molecules.
Identification of a deoxyribonuclease I-like endonuclease in rat granulosa and luteal cell nuclei.

Apoptosis, a process recently implicated as the cellular mechanism underlying ovarian follicular atresia and luteal regression, is characterized by the internucleosomal degradation of DNA by a Ca2+/Mg(2+)-dependent endonuclease. Although hormones and growth factors have been demonstrated to modulate the DNA degradation associated with ovarian follicular apoptosis, the nature and identity of the endonuclease involved is not known. Ca2+/Mg(2+)-dependent endonuclease activity has a developmental pattern of expression in rat granulosa and luteal cell nuclei. Thus, the present study was conducted to establish the presence of an endonuclease in the nuclei of ovarian granulosa and luteal cells and to examine the biochemical properties of the enzyme relevant to apoptosis. Nuclei from diethyl-stilbestrol (DES)-, eCG-, and hCG-primed rat ovaries were isolated and exposed to Ca2+ and Mg2+ in vitro. Nuclei from rat ovaries primed with eCG and hCG, but not DES, substantially degraded their DNA in an apoptotic fashion, and this DNA degradation was Ca2+/Mg(2+)-dependent and inhibited by Zn2+. Protein extracts from the nuclei of DES-, eCG-, and hCG-treated rat ovaries were tested for endonuclease activity by a plasmid degradation assay. The extracts were found to contain endonuclease activity with the same developmental pattern and cation dependency as found in intact nuclei. These protein extracts were assessed for nuclease activity by zymography, and three nuclease activities were identified depending on the type of DNA used in the gel and the electrophoresis conditions used for protein separation. (ABSTRACT TRUNCATED AT 250 WORDS)
Does the source matter?

This article provides an insight into the expression of reportative evidentiality in Spanish scientific articles published between 1799 and 1920. Central to the discussion is the presence and specificity of sources in reportative constructions. While contemporary scientific discourse prioritizes the use of specific, reportative-quotative evidentials, this is not a constant feature of articles analyzed in this study. In order to trace this historical variation, we established a classification of reportative constructions according to the specificity of the evidence they convey and we conducted both qualitative and quantitative analyses. According to our results, different specificity patterns were prominent in different temporal stages of the period under review. We argue that this can be interpreted in light of the growth and changing practices of the scientific community.
Antecedents and outcomes of retailer reputation: Evidence from an emerging economy

Over prevous years the construct of corporate reputation has received much attention among academics and business practitioners. Whereas researchers’ interests have been mainly pointed to the implications of good corporate reputation, its antecedents have largly been understudied. Moreover, majority of previous research took part in the context of the U.S. and Western Europe, leaving service industries in emerging economies rather neglected area. Hoping to fill this void in marketing knowledge, the objective of this study is to propose and empirically examine a model of retailer reputation, by incorporating the construct into the framework of established relationships among key service evaluation constructs. Conceptual model has been examined on a sample of Serbian grocery retail customers by means of structural equation modelling (SEM). Results of the study point to significant effect of service quality and value on retailer reputation and the direct impact of reputation on customer loyalty. Limitations of the study have been discussed and directions for future research are provided.
Autologous Chondrocyte Implantation: Superior Biologic Properties of Hyaline Cartilage Repairs

Information regarding the quality of autologous chondrocyte implantation repair is needed to determine whether the current autologous chondrocyte implantation surgical technology and the subsequent biologic repair processes are capable of reliably forming durable hyaline or hyaline-like cartilage in vivo. We report and analyze the properties and qualities of autologous chondrocyte implantation repairs. We evaluated 66 autologous chondrocyte implantation repairs in 57 patients, 55 of whom had histology, indentometry, and International Cartilage Repair Society repair scoring at reoperation for mechanical symptoms or pain. International Knee Documentation Committee scores were used to address clinical outcome. Maximum stiffness, normalized stiffness, and International Cartilage Repair Society repair scoring were higher for hyaline articular cartilage repairs compared with fibrocartilage, with no difference in clinical outcome. Reoperations revealed 32 macroscopically abnormal repairs (Group B) and 23 knees with normal-looking repairs in which symptoms leading to arthroscopy were accounted for by other joint disorders (Group A). In Group A, 65% of repairs were either hyaline or hyaline-like cartilage compared with 28% in Group B. Autologous chondrocyte repairs composed of fibrocartilage showed more morphologic abnormalities and became symptomatic earlier than hyaline or hyaline-like cartilage repairs. The hyaline articular cartilage repairs had biomechanical properties comparable to surrounding cartilage and superior to those associated with fibrocartilage repairs.
Low complexity embedded compression algorithm for reduction of memory size and bandwidth requirements in the JPEG2000 encoder

In this paper, a new and low-complexity embedded compression (EC) algorithm for the JPEG2000 encoder system is proposed to efficiently reduce memory requirements. The proposed EC algorithm is used to achieve a fixed compression ratio of 50% under the near-lossless compression constraint. Through the EC technique, the memory requirement for intermediate low-frequency coefficients during multiple DWT stages can be reduced by a factor of 2 compared with direct implementation of the JPEG2000 encoder. By jointly considering the coding flow of both block-based 2D-DWT and bit-plane coders, the EC scheme combines an efficient quantization and simple entropy coder to reduce memory transaction bandwidth between DWT and bit-plane coder (BPC) to 50%. Furthermore, this EC reduces the size of code-block memory from DWT to BPC. Experiment results based on standard test image benchmarks show that our proposed EC algorithm presents only PSNR degradation of 1.05 dB on average when the target compression ratio is fixed at 50%. Our EC can be applicable to the JPEG2000 encoding system to save memory size and bandwidth requirements with minor picture quality degradation. The proposed EC algorithm is also computationally simple, reducing latency time and creating sufficient accessibility.
Defective calcium homeostasis in the cerebellum in a mouse model of Niemann–Pick A disease

We recently demonstrated that calcium homeostasis is altered in mouse models of two sphingolipid storage diseases, Gaucher and Sandhoff diseases, owing to modulation of the activities of a calcium‐release channel (the ryanodine receptor) and of the sarco/endoplasmic reticulum Ca2+‐ATPase (SERCA) respectively, by the accumulating sphingolipids. We now demonstrate that calcium homeostasis is also altered in a mouse model of Niemann–Pick A disease, the acid sphingomyelinase (A‐SMase)‐deficient mouse (ASM–/–), with reduced rates of calcium uptake via SERCA in the cerebellum of 6–7‐month‐old mice. However, the mechanism responsible for defective calcium homeostasis is completely different from that observed in the other two disease models. Thus, levels of SERCA expression are significantly reduced in the ASM–/– cerebellum by 6–7 months of age, immediately before death of the mice, as are levels of the inositol 1,4,5‐triphosphate receptor (IP3R), the major calcium‐release channel in the cerebellum. Systematic analyses of the time course of loss of SERCA and IP3R expression revealed that loss of the IP3R preceeded that of SERCA, with essentially no IP3R remaining by 4 months of age, whereas SERCA was still present even after 6 months. Expression of zebrin II (aldolase C), a protein found in about half of the Purkinje cells in the adult mouse cerebellum, was essentially unchanged during development. We discuss possible pathological mechanisms related to calcium dysfunction that may cause Purkinje cell degeneration, and as a result, the onset of neuropathology in Niemann–Pick A disease.
Open and inclusive collaboration in science

Digitalisation is fundamentally changing science and the paper lays out some of the opportunities, risks and major policy challenges associated with these changes. More specifically, the paper lays out a conceptual framework for open science. This framework incorporates access to data and information, as well as civil society engagement, in the different stages of the scientific research process. It is not meant to be prescriptive but should help different communities to decide on their own priorities within the open science space and to better visualise how these priorities link to different stage of the scientific process and to different actors. Such a framework can be useful also in considering how best to incentivise and measure different aspects of open science.
High-throughput Titration of Luciferase-expressing Recombinant Viruses

Standard plaque assays to determine infectious viral titers can be time consuming, are not amenable to a high volume of samples, and cannot be done with viruses that do not form plaques. As an alternative to plaque assays, we have developed a high-throughput titration method that allows for the simultaneous titration of a high volume of samples in a single day. This approach involves infection of the samples with a Firefly luciferase tagged virus, transfer of the infected samples onto an appropriate permissive cell line, subsequent addition of luciferin, reading of plates in order to obtain luminescence readings, and finally the conversion from luminescence to viral titers. The assessment of cytotoxicity using a metabolic viability dye can be easily incorporated in the workflow in parallel and provide valuable information in the context of a drug screen. This technique provides a reliable, high-throughput method to determine viral titers as an alternative to a standard plaque assay.
Representation of online image of tourist destination: a content analysis of Huangshan

ABSTRACT Online image of tourist destination has emerged as a crucial element in the construction of tourist destination image. The destination, along with the online tourism marketers, plays an increasingly significant role in forming online image of tourist destination. This study takes Huangshan as an example and collects photographic and textual information of Huangshan from its official online media and online tourism marketers. Built upon the semiotic theory, content analysis, and visual analysis are employed to explore the online image of Huangshan. The analysis comprises the coding manual formation, coding check, and formal encoding. The results indicate that online image of tourist destination of Huangshan is composed of three elements, that is, tourism resources, tourism facilities, and tourism services, which further comprise several secondary elements. Different information sources with different demonstration focuses of online image of tourist destination elements are different. Consistency tendency is in common among the demonstration of online image of tourist destination elements in different media forms.
A Response Surface Methodology Approach to Investigate the Effect of Sulfur Dioxide, pH, and Ethanol on DbCD and DbVPR Gene Expression and on the Volatile Phenol Production in Dekkera/Brettanomyces bruxellensis CBS2499

Dekkera/Brettanomyces bruxellensis, the main spoilage yeast in barrel-aged wine, metabolize hydroxycinnamic acids into off-flavors, namely ethylphenols. Recently, both the enzymes involved in this transformation, the cinnamate decarboxylase (DbCD) and the vinylphenol reductase (DbVPR), have been identified. To counteract microbial proliferation in wine, sulfur dioxide (SO2) is used commonly to stabilize the final product, but limiting its use is advised to preserve human health and boost sustainability in winemaking. In the present study, the influence of SO2 was investigated in relation with pH and ethanol factors on the expression of DbCD and DbVPR genes and volatile phenol production in D. bruxellensis CBS2499 strain under different model wines throughout a response surface methodology (RSM). In order to ensure an exact quantification of DbCD and DbVPR expression, an appropriate housekeeping gene was sought among DbPDC, DbALD, DbEF, DbACT, and DbTUB genes by GeNorm and Normfinder algorithms. The latter gene showed the highest expression stability and it was chosen as the reference housekeeping gene in qPCR assays. Even though SO2 could not be commented as main factor because of its statistical irrelevance on the response of DbCD gene, linear interactions with pH and ethanol concurred to define a significant effect (p < 0.05) on its expression. The DbCD gene was generally downregulated respect to a permissive growth condition (0 mg/L mol. SO2, pH 4.5 and 5% v/v ethanol); the combination of the factor levels that maximizes its expression (0.83-fold change) was calculated at 0.25 mg/L mol. SO2, pH 4.5 and 12.5% (v/v) ethanol. On the contrary, DbVPR expression was not influenced by main factors or by their interactions; however, its expression is maximized (1.80-fold change) at the same conditions calculated for DbCD gene. While no linear interaction between factors influenced the off-flavor synthesis, ethanol and pH produced a significant effect as individual factors. The obtained results can be useful to improve the SO2 management at the grape harvesting and during winemaking in order to minimize the D. /B. bruxellensis spoilage.