Miracle-dz/UniFAR-FTUs huggingface dataset:
Genomic characterization of sex‐identification markers in Sebastes carnatus and Sebastes chrysomelas rockfishes

Fish have evolved a variety of sex‐determining (SD) systems including male heterogamy (XY), female heterogamy (ZW) and environmental SD. Little is known about SD mechanisms of Sebastes rockfishes, a highly speciose genus of importance to evolutionary and conservation biology. Here, we characterize the sex determination system in the sympatrically distributed sister species Sebastes chrysomelas and Sebastes carnatus. To identify sex‐specific genotypic markers, double digest restriction site – associated DNA sequencing (ddRAD‐seq) of genomic DNA from 40 sexed individuals of both species was performed. Loci were filtered for presence in all of the individuals of one sex, absence in the other sex and no heterozygosity. Of the 74 965 loci present in all males, 33 male‐specific loci met the criteria in at least one species and 17 in both. Conversely, no female‐specific loci were detected, together providing evidence of an XY sex determination system in both species. When aligned to a draft reference genome from Sebastes aleutianus, 26 sex‐specific loci were interspersed among 1168 loci that were identical between sexes. The nascent Y chromosome averaged 5% divergence from the X chromosome and mapped to reference Sebastes genome scaffolds totalling 6.9Mbp in length. These scaffolds aligned to a single chromosome in three model fish genomes. Read coverage differences were also detected between sex‐specific and autosomal loci. A PCR‐RFLP assay validated the bioinformatic results and correctly identified sex of five additional individuals of known sex. A sex‐determining gene in other teleosts gonadal soma‐derived factor (gsdf) was present in the model fish chromosomes that spanned our sex‐specific markers.
VALVULAR AND STRUCTURAL HEART DISEASES Original Studies Blood Loss and Transfusion Rates Associated with Transcatheter Aortic Valve Replacement: Recommendations for Patients Who Refuse Blood Transfusion

Background: Transcatheter aortic valve replacement (TAVR) is less invasive than surgical aortic valve replacement and may be preferred for patients who refuse blood transfusions. Objectives: Our study sought to define transfusion rates in TAVR, identify predictors and develop recommendations for patients who refuse transfusions. Methods: A large cohort of consecutive patients undergoing TAVR was prospectively studied. Blood loss and transfusion rates were observed in patients undergoing transfemoral (TF-TAVR) and transapical TAVR (TA-TAVR). Predictors for transfusion were investigated in a multivariate model. Results: Of 373 consecutive patients, 270 underwent TF-TAVR and 103 TA-TAVR. Transfusion rates were significantly lower in TF-TAVR than TA-TAVR (11.1 vs 41.7%; P < 0.001). In patients who did not receive transfusions, blood loss was significantly lower in TF-TAVR than TA-TAVR (23.6 6 12.2 g/l vs 28.9 6 13.7 g/l; P 5 0.004), but did not differ in transfused patients (36.9 6 12.9 g/l vs 33.4 6 21.2 g/l; P 5 0.428). Predictors for transfusions were low baseline hemoglobin, female sex, low body weight and decreased renal function. In seven Jehovah’s Witness patients, who refused transfusions, no vascular complications occurred and clinical outcome was excellent. Conclusion: In patients, who refuse transfusions, TAVR may be performed with good clinical outcomes provided a high baseline hemoglobin level, careful management of the access site and strict measurements to reduce blood loss. TF-TAVR is associated with less blood loss and a lower rate of transfusions than TA-TAVR, and may be the preferred option for patients, who refuse transfusions. VC 2014 Wiley Periodicals, Inc.
Development of a Novel Hybrid Optimization Algorithm for Minimizing Irrigation Deficiencies

One of the most important issues in the field of water resource management is the optimal utilization of dam reservoirs. In the current study, the optimal utilization of the Aydoghmoush Dam Reservoir is examined based on a hybrid of the bat algorithm (BA) and particle swarm optimization algorithm (PSOA) by increasing the convergence rate of the new hybrid algorithm (HA) without being trapped in the local optima. The main goal of the study was to reduce irrigation deficiencies downstream of this reservoir. The results showed that the HA reduced the computational time and increased the convergence rate. The average downstream irrigation demand over a 10-year period (1991–2000) was 25.12 × 106 m3, while the amount of water release based on the HA was 24.48 × 106 m3. Therefore, the HA was able to meet the irrigation demands better than some other evolutionary algorithms. Moreover, lower indices of root mean square error (RMSE) and mean absolute error (MAE) were obtained for the HA. In addition, a multicriteria decision-making model based on the vulnerability, reliability, and reversibility indices and the objective function performed better with the new HA than with the BA, PSOA, genetic algorithm (GA), and shark algorithm (SA) in terms of providing for downstream irrigation demands.
Evaluation of the Developmental Toxicity of Linalool in Rats

The developmental toxicity of linalool, a widely used fragrance ingredient, was evaluated in presumed pregnant Sprague-Dawley rats (25/group). Oral dosages of 0, 250, 500, or 1000 mg/kg/day linalool were administered by gavage on gestational days 7 to 17. The presence of spermatozoa and/or a copulatory plug in situ was designated as gestational day 0. Rats were observed for viability, clinical signs, body weights, and feed consumption. Caesarean sectioning and necropsy occurred on gestational day 21. Uteri were examined for number and distribution of implantations, live and dead fetuses, and early and late resorptions. Numbers of corpora lutea were also recorded. Fetuses were weighed and examined for gender, gross external changes, and soft tissue or skeletal alterations. There were no maternal deaths, clinical signs, or gross lesions that were considered related to linalool. During the dosage period, mean relative feed consumption was significantly reduced by 7% and mean body weight gains were reduced by 11% at 1000 mg/kg/day. During the postdosage period, feed consumption values at 1000 mg/kg/day were significantly higher than vehicle control values, which corresponded to the increase in body weight gains during this period. Caesarean section and litter parameters, as well as fetal alterations, were not affected by linalool at any of the three dosages tested. On the basis of these data, the maternal no observed adverse effect level (NOAEL) of linalool is 500 mg/kg/day, whereas the developmental NOAEL is ≥ 1000 mg/kg/day. It is concluded that linalool is not a developmental toxicant in rats at maternal doses of up to 1000 mg/kg/day.
Effect of anti‐IgE in occupational asthma caused by exposure to low molecular weight agents

The role of immunoglobulin (Ig)‐E in occupational asthma (OA) due to low molecular weight (LMW) agents is not well established compared to classical atopic asthma. In this study, we evaluate whether anti‐IgE monoclonal antibody (mAb) has an effect in a mouse model of OA, using persulfate salts.
The role of design factors in influencing training transfer among small businesswomen

The objective of this research is to investigate the effect of design factors which consist of training content, training delivery, trainer competency and opportunity to use on small businesswomen's goal setting activities. The instrument for this research is adapted and modified from the Training Transfer Model and Model for Excellence (American Society of Training and Development Competency Research). Four independent variables: training content, training delivery and trainer's competency and opportunity to use; and goal setting as dependent variable formed the framework for this research. Multiple regressions were used to investigate the relationship between design factors and goal setting. Findings from a survey of 246 small businesswomen attending training programs organized by government agencies showed that opportunity to use made the strongest contribution towards goal setting followed by training content, trainer's competency and training delivery. Awareness on the constraints or barriers in design factors can assist the primary stakeholders (organizer and trainers) and human resource personnel in developing effective training programs which are expected to create a fair situation for them to accomplish their training objectives as well as beneficial to trainees to transfer the knowledge and skills to their own businesses.
SWGDAM Interpretation Guidelines for Autosomal STR Typing by Forensic DNA Testing Laboratories

The Scientific Working Group on DNA Analysis Methods, better known by its acronym of SWGDAM, is a group of scientists representing federal, state, and local forensic DNA laboratories in the United States and Canada. During meetings, which are held twice a year, subcommittees discuss topics of interest to the forensic DNA community and often develop documents to provide direction and guidance for the community. These guidelines were presented to the SWGDAM membership and approved on January 12, 2017.
Nursing resources: a major determinant of nosocomial infection?

Purpose of review There is growing concern that changes in nurse workforce and hospital-restructuring interventions negatively impact on patient outcomes. This review focuses on the association between understaffing and health-care-associated infections. Recent findings There is a large number of studies showing that overcrowding, understaffing or a misbalance between workload and resources are important determinants of nosocomial infections and cross-transmission of microorganisms. Importantly, not only the number of staff but also the level of their training affects outcomes. The nurse workforce is ageing, mainly due to fewer individuals' engaging in a nursing career. This phenomenon, combined with cost-driven downsizing, contributes to a nursing shortage, and this tendency is not expected to revert unless important system changes are implemented. The causal pathway between understaffing and infection is complex, and factors might include lack of time to comply with infection control recommendations, job dissatisfaction, job-related burnout, absenteeism and a high staff turnover. Summary The evidence that cost-driven downsizing and changes in staffing patterns causes harm to patients cannot be ignored, and should not be considered as an inevitable outcome. More research is needed to better define the optimal patient-to-nurse ratio in various hospital settings and to estimate the economical impact of the nursing shortage. All quality-improvement interventions should carefully take into account systems and processes to be successful, as the issue of staffing is essentially a structural problem.
Automated weather event analysis with machine learning

Weather forecasting has numerous impacts in our daily life from cultivation to event planning. Previous weather forecasting models used the complicated blend of mathematical instruments which was insufficient in order to get higher classification rate. In contrast, simple analytical models are well-suited for weather forecasting tasks. In this work, we focus on the weather forecasting by means of classifying different weather events such as normal, rain, and fog by applying comprehensible C4.5 learning algorithm on weather and climate features. The C4.5 classifier classifies weather events by building the decision tree using information entropy from the set of training samples. We conducted experiments on LA weather history dataset; from evaluation results, it is revealed that C4.5 classifier classifies weather events with f-score of around 96.1%. This model also indicates that climate features such as rainfall, visibility, temperature, humidity, and wind speed are highly discriminative toward events classification.
Effect of nonspecific phospholipid transfer protein on cholesterol esterification in microsomes from Morris hepatomas.

The content of cholesterol and cholesterol ester as well as the levels of acyl coenzyme A:cholesterol acyltransferase activity were determined in the microsomes from Morris hepatomas 7777, 5123D, and 7787. The free cholesterol content, expressed per mg microsomal protein, was significantly increased only in the microsomes from Morris hepatoma 7777 [47.8 +/- 0.4 micrograms (S.D.); p less than 0.001] and hepatoma 7787 (37.6 +/- 6.2 micrograms; p less than 0.01) as compared to normal liver (28.8 +/- 2.4 micrograms). The cholesterol ester content in the microsomes of the three different tumors did not significantly differ from that of normal liver (2.1 +/- 1.2 micrograms cholesterol per mg microsomal protein). The microsomal acyl coenzyme A:cholesterol acyltransferase activity was decreased in Morris hepatoma 7777 (8.6 +/- 2.3 pmol/min/mg protein; p less than 0.01) and in hepatoma 5123D (7.5 +/- 1.7 pmol/min/mg protein; p less than 0.02), and was normal in the hepatoma 7787 (16.5 +/- 7.8 pmol/min/mg protein) as compared to rat liver (16.0 +/- 2.9 pmol/min/mg protein). In a previous study (B. J. H. M. Poorthuis and K. W. A. Wirtz, Biochim. Biophys. Acta, 710: 99-105, 1982), this acyltransferase activity was shown to be stimulated by preincubation of rat liver microsomes with cholesterol-containing vesicles and the nonspecific phospholipid transfer protein. In this paper, a similar 4-fold stimulation of activity was observed for the microsomes of the various hepatomas investigated. The possible role of the nonspecific phospholipid transfer protein in intracellular cholesterol esterification is discussed.
A Verification Guided Approach for Selective Program Transformations for Approximate Computing

In recent times, approximate computing is being looked at as a viable alternative for reducing the energy consumption of programs, while marginally compromising on the correctness of their computation. The idea behind approximate computing is to introduce approximations at various levels of the execution stack, with an attempt to realize the resource hungry computations on low resource consuming approximate hardware blocks. However approximate computing for program transformation faces a serious challenge of automatically identifying core program areas/statements where approximations can be introduced, with a quantifiable measure of the resulting program correctness compromise. Introducing approximations randomly can cause performance deterioration without much energy advantage, which is undesirable. In this paper, we introduce a verification-guided method to automatically identify program blocks which lend themselves to easy approximations, while not compromising significantly on program correctness. Our method is based on identifying regions of code which are less influential for the computation of the program outputs and therefore, can be compromised with, however still having a potential of significant resource reduction. We take the help of assertions to quantify the effect of the resulting transformations on program outputs. We show experimental results to support our proposal.
Does Writing Affect Asthma? A Randomized Trial

Objective: Nonpharmacologic treatments for asthma may act as useful adjuncts to pharmacotherapy but should be recommended to patients only after several well-controlled studies provide evidence of efficacy. Research demonstrating that written emotional expression can improve pulmonary function in patients with asthma consists of one impressive yet unreplicated study (1). Our main objective was to test and extend previous research finding that written emotional expression improves pulmonary function in patients with asthma compared with writing on neutral topics. Methods: We conducted a randomized, controlled trial of outpatient asthmatics recruited from hospitals and the community. Of the 137 adult patients with asthma who were randomized, 117 began and 114 completed the study. Patients were randomly assigned to write for 20 minutes, once per week, for 3 weeks about stressful experiences (n = 41), positive experiences (n = 37), or neutral experiences (n = 36; control group). At baseline, postintervention, and 2-month follow up, patients were assessed by spirometry. Results: The mean change from baseline to 2-month follow up in percentage of predicted forced expiratory volume in 1 second (FEV1) was 4.2% in the stress-writing group, 1.3% in the positive-writing group, and 3.0% in the control group. In forced vital capacity (FVC), there was 3.1% improvement in the stress-writing group, 3.6% in the positive-writing group, and 2.4% in the control group. These changes were not statistically or clinically significant. Conclusions: The present study reduces confidence in the ability of written emotional expression to benefit the disease status of asthma patients. FEV1 = forced expiratory volume in 1 second; FVC = forced vital capacity; VAPAHCS = Veteran Affairs Palo Alto Health Care System; PTSD = posttraumatic stress disorder; COPD = chronic obstructive pulmonary disease.
Reliability and generality of measures of acceleration, planned agility, and reactive agility.

PURPOSE The purpose of the current study was to assess the reliability of a new protocol that examines different components of agility using commercially available timing gates. METHODS Seventeen physically active males completed four trials of a new protocol, which consisted of a number of 10-m sprints. Sprints were completed in a straight line or with a change of direction after 5 m. The change of direction was either planned or reactive, with participants reacting to a visual light stimulus. RESULTS There was no systematic bias in any of the measures, although random variation was reduced in the straight acceleration and planned agility when considering only the final pair of trials, with mean coefficients of variation (CV) of 1.6% (95% CI, 1.2% to 2.4%) and 1.1% (0.8% to 1.7%), respectively. Reliability of reactive agility remained consistent throughout with mean CVs of approximately 3%. Analyses revealed a high degree of common variance between acceleration times and both planned (r2 = .93) and reactive (r2 = .83) agility, as well as between the two agility protocols (r2 = .87). CONCLUSION Both planned and reactive agility could be measured reliably. Protocol design and use of a light stimulus in the reactive test emphasize physical abilities comparable with other test measures. Therefore, inclusion of a reactive light stimulus does not appear to require any additional perceptual qualities.
Pattern of blood pressure and hypertension in adolescents in Port Harcourt, Nigeria.

BACKGROUND The rate and prevalence of hypertension in children is increasing. Childhood hypertensionif untreated can lead to hypertension in adulthood with its consequent cardiovascular and renal complications. Early detection of paediatric hypertension may lead to improvement in cardiovascular health in adults. OBJECTIVE This study aims to determine the blood pressure (BP) pattern and prevalence of hypertension in asymptomatic secondary school children and factors associated with hypertension in these adolescents. METHODS A cross-sectional study of 820 adolescents selected from 12 secondary schools in Port Harcourt was conducted. BP was measured by the auscultatory method. The average of three readings was taken as the actual blood pressure. Hypertension was defined as systolic and/or diastolic blood pressures equal to or greater than the 95th percentile for age, sex and height. Data was analysed using SPSS version 17.0. RESULTS Systolic and diastolic BP increased with age in all subjects. Male subjects had a higher systolic BP compared to females. Hypertension was seen in 26 (3.2%) subjects; 13 males and 13 females. The proportional prevalence was higher in the age group 15-17 years (3.9%); in those in social classes V (9.1%) and with family history of hypertension (3.6%), (p Ã 0.05 in all cases). It was however significantly higher in the obese subjects (p = 0.000). CONCLUSION The prevalence of hypertension in adolescents in Port Harcourt is high and is strongly associated with obesity. We recommend blood pressure measurement as part of the school health programme in secondary schools.
Meta-analysis shows that detection of circulating tumor cells indicates poor prognosis in patients with colorectal cancer.

BACKGROUND & AIMS The prognostic significance of circulating (CTCs) and disseminated tumor cells in patients with colorectal cancer (CRC) is controversial. We performed a meta-analysis of available studies to assess whether the detection of tumor cells in the blood and bone marrow (BM) of patients diagnosed with primary CRC can be used as a prognostic factor. METHODS We searched the Medline, Biosis, Science Citation Index, and Embase databases and reference lists of relevant articles (including review articles) for studies that assessed the prognostic relevance of tumor cell detection in the peripheral blood (PB), mesenteric/portal blood (MPB), or BM of patients with CRC. Meta-analyses were performed using a random effects model, with hazard ratio (HR) and 95% confidence intervals (95% CIs) as effect measures. RESULTS A total of 36 studies, including 3094 patients, were eligible for final analyses. Pooled analyses that combined all sampling sites (PB, MPB, and BM) associated the detection of tumor cells with poor recurrence-free survival (RFS) (HR = 3.24 [95% CI: 2.06-5.10], n = 26, I(2) = 77%) and overall survival (OS) (2.28 [1.55-3.38], n = 21, I(2) = 66%). Stratification by sampling site showed that detection of tumor cells in the PB compartment was a statistically significant prognostic factor (RFS: 3.06 [1.74-5.38], n = 19, I(2) = 78%; OS: 2.70 [1.74-4.20], n = 16, I(2) = 59%) but not in the MPB (RFS: 4.12 [1.01-16.83], n = 8, I(2) = 75%; OS: 4.80 [0.81-28.32], n = 5, I(2) = 82%) or in the BM (RFS: 2.17 [0.94-5.03], n = 4, I(2) = 78%; OS: 1.50 [0.52-4.32], n = 3, I(2) = 84%). CONCLUSION Detection of CTCs in the PB indicates poor prognosis in patients with primary CRC.
The Prevalence and Characteristics of Thoracic Magnetic Resonance Imaging Findings in Men

Study Design. A cross-sectional study of thoracic magnetic resonance image (MRI) findings. Objective. To examine the prevalence of different thoracic MRI findings for T6–T12 and their associations with age and one another by level. Summary of Background Data. There is a dearth of descriptive epidemiology of thoracic MRI findings in the general population. Methods. Thoracic MRIs of 524 men were assessed qualitatively and quantitatively for a variety of findings, including disc bulging, height and signal, vertebral deformities, endplate irregularities, osteophytes, and hemangiomas. Descriptive statistics, correlation coefficients and STATA's survey analysis were used. Results. In the lower thoracic spine, 5.4% to 9.5% of the discs, depending on level, were qualitatively assessed as moderately to severely narrowed. Anterior bulging was more common than posterior, which was relatively rare and mild when present. Signal was lower in the midthoracic than lower discs. At least 1 moderate or severe vertebral deformity was found in 6.1% of the subjects, suggesting fracture, and hemangiomas were identified in 2.3% of subjects. Disc signal correlated most highly with age (r = 0.31–0.42). Qualitatively assessed disc height narrowing (r = 0.29–0.46) and quantitative disc height (r = 0.11–0.29) were associated with disc signal. Upper and lower endplate irregularities were associated with one another (r = 0.17–0.32), as were bulging and osteophytes, anteriorly (r = 0.35–0.61) and posteriorly (r = 0.26–0.45). Conclusion. Degenerative MRI findings beyond a mild grade were not commonly observed in the thoracic spine among 35–70-year-old men. Posterior bulges, in particular, were rare. The highest correlation with age existed for disc signal. Different MRI findings were associated with one another, but the magnitude of association varied by level. The effects of individual judgments and disc level on prevalence rates were apparent.